LOCUS       DACENJ010000001        51047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.001, whole genome shotgun sequence.
ACCESSION   DACENJ010000001 DACENJ010000000
VERSION     DACENJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.001"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            75..920
                     /locus_tag="IW748_000001"
     CDS             75..920
                     /locus_tag="IW748_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1102941.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1201..2421
                     /locus_tag="IW748_000002"
     CDS             1201..2421
                     /locus_tag="IW748_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1102942.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2418..5138
                     /locus_tag="IW748_000003"
     CDS             2418..5138
                     /locus_tag="IW748_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1102943.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5122..6066
                     /locus_tag="IW748_000004"
     CDS             5122..6066
                     /locus_tag="IW748_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1102944.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6135..7145)
                     /locus_tag="IW748_000005"
     CDS             complement(6135..7145)
                     /locus_tag="IW748_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1102945.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7325..7669)
                     /locus_tag="IW748_000006"
     CDS             complement(7325..7669)
                     /locus_tag="IW748_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1102946.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7662..8096)
                     /locus_tag="IW748_000007"
     CDS             complement(7662..8096)
                     /locus_tag="IW748_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1102947.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8234..8971
                     /locus_tag="IW748_000008"
     CDS             8234..8971
                     /locus_tag="IW748_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1102948.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8964..10175
                     /locus_tag="IW748_000009"
     CDS             8964..10175
                     /locus_tag="IW748_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1102949.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10382..11161
                     /locus_tag="IW748_000010"
     CDS             10382..11161
                     /locus_tag="IW748_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1102950.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11231..11893
                     /gene="trmB"
                     /locus_tag="IW748_000011"
     CDS             11231..11893
                     /gene="trmB"
                     /locus_tag="IW748_000011"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1102951.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11991..13745)
                     /gene="ade"
                     /locus_tag="IW748_000012"
     CDS             complement(11991..13745)
                     /gene="ade"
                     /locus_tag="IW748_000012"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1102952.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13877..14806
                     /gene="cpaF"
                     /locus_tag="IW748_000013"
     CDS             13877..14806
                     /gene="cpaF"
                     /locus_tag="IW748_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1102953.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14763..15812
                     /locus_tag="IW748_000014"
     CDS             14763..15812
                     /locus_tag="IW748_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1102954.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15809..16087
                     /locus_tag="IW748_000015"
     CDS             15809..16087
                     /locus_tag="IW748_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1102955.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16062..16532
                     /locus_tag="IW748_000016"
     CDS             16062..16532
                     /locus_tag="IW748_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1102956.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16549..16851
                     /locus_tag="IW748_000017"
     CDS             16549..16851
                     /locus_tag="IW748_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1102957.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16868..17239
                     /locus_tag="IW748_000018"
     CDS             16868..17239
                     /locus_tag="IW748_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1102958.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17211..17570
                     /locus_tag="IW748_000019"
     CDS             17211..17570
                     /locus_tag="IW748_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1102959.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17742..18749
                     /locus_tag="IW748_000020"
     CDS             17742..18749
                     /locus_tag="IW748_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1102960.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18774..19958
                     /locus_tag="IW748_000021"
     CDS             18774..19958
                     /locus_tag="IW748_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1102961.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20395..21978)
                     /locus_tag="IW748_000022"
     CDS             complement(20395..21978)
                     /locus_tag="IW748_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1102962.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEVTVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22556..22699)
                     /locus_tag="IW748_000023"
     CDS             complement(22556..22699)
                     /locus_tag="IW748_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1102963.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22922..23401)
                     /locus_tag="IW748_000024"
     CDS             complement(22922..23401)
                     /locus_tag="IW748_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1102964.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23474..23758)
                     /locus_tag="IW748_000025"
     CDS             complement(23474..23758)
                     /locus_tag="IW748_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1102965.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23836..24030)
                     /gene="ssrS"
                     /locus_tag="IW748_000026"
     ncRNA           complement(23836..24030)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW748_000026"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24102..25388)
                     /locus_tag="IW748_000027"
     CDS             complement(24102..25388)
                     /locus_tag="IW748_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1102966.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKSAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25526..26197
                     /locus_tag="IW748_000028"
     CDS             25526..26197
                     /locus_tag="IW748_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1102967.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26163..26651
                     /locus_tag="IW748_000029"
     CDS             26163..26651
                     /locus_tag="IW748_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1102968.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26664..27611
                     /gene="prmA"
                     /locus_tag="IW748_000030"
     CDS             26664..27611
                     /gene="prmA"
                     /locus_tag="IW748_000030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1102969.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27612..28376
                     /locus_tag="IW748_000031"
     CDS             27612..28376
                     /locus_tag="IW748_000031"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1102970.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28609..30822
                     /locus_tag="IW748_000032"
     CDS             28609..30822
                     /locus_tag="IW748_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1102971.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30833..31279
                     /locus_tag="IW748_000033"
     CDS             30833..31279
                     /locus_tag="IW748_000033"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1102972.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31450..32709
                     /locus_tag="IW748_000034"
     CDS             31450..32709
                     /locus_tag="IW748_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1102973.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32798..33745)
                     /locus_tag="IW748_000035"
     CDS             complement(32798..33745)
                     /locus_tag="IW748_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1102974.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34139..35431
                     /locus_tag="IW748_000036"
     CDS             34139..35431
                     /locus_tag="IW748_000036"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1102975.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35446..37215
                     /gene="aspS"
                     /locus_tag="IW748_000037"
     CDS             35446..37215
                     /gene="aspS"
                     /locus_tag="IW748_000037"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1102976.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37324..38199
                     /locus_tag="IW748_000038"
     CDS             37324..38199
                     /locus_tag="IW748_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1102977.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38465..40348
                     /locus_tag="IW748_000039"
     CDS             38465..40348
                     /locus_tag="IW748_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1102978.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40423..40707)
                     /locus_tag="IW748_000040"
     CDS             complement(40423..40707)
                     /locus_tag="IW748_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1102979.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40697..41431)
                     /locus_tag="IW748_000041"
     CDS             complement(40697..41431)
                     /locus_tag="IW748_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1102980.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41560..42183
                     /locus_tag="IW748_000042"
     CDS             41560..42183
                     /locus_tag="IW748_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1102981.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42375..43496
                     /locus_tag="IW748_000043"
     CDS             42375..43496
                     /locus_tag="IW748_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1102982.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43503..46631
                     /locus_tag="IW748_000044"
     CDS             43503..46631
                     /locus_tag="IW748_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1102983.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46650..49844
                     /locus_tag="IW748_000045"
     CDS             46650..49844
                     /locus_tag="IW748_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1102984.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49990..50697)
                     /locus_tag="IW748_000046"
     CDS             complement(49990..50697)
                     /locus_tag="IW748_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1102985.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16703 a         8930 c        10610 g        14804 t
ORIGIN      
        1 tataattagt atatgttttg tgagaaattt agacttacaa taagcagtga atttataatc
       61 aacgtatagg agacatggat gaaatgaaac taattggtat agatttagac ggtactcttt
      121 tgaattctga ccagagcatt agtcaaaaaa atgcaagaac aatgaaacat ttaccggaag
      181 actgttttcc attcatttgt tctggaagag aagtagaaga cattaataat attctcaaaa
      241 aatctggttt aaggatacca gcagtagggt taaatggttc tgttggattt gatggagaaa
      301 aaaagatatt tgagtcttcc ttcgataaaa atgatgtaaa aaaaattgat tctgtcctat
      361 cgaaatttcc aaccaaaata tatacaaatc tcggaagtta tgaatctcag aattataaaa
      421 ataaaatgaa aaaaattttt cacgaggtag ggagtgaatt ttctatcaaa gagctaaact
      481 atgagcttga gtatgaaaaa tctatttgtt caatccctta taataatatt gaggaaatta
      541 tagaaaagga tgctatcagc atttacaaat tttttatttt tattcctaat cgtcaattga
      601 aagctaatat atttaaccat ttgaaaaaga tgctaaatgt gtctgttact gaatcggcac
      661 ctgtaaatat tgaaatcgta cctcaaaatg tatctaaagg tgtggtgttt gatcgtttgg
      721 aatcaatcta caatttaaag aaacctttga gaatcgcgat cggagatagt ttaaatgact
      781 tatctatgtt tgaaagtgca gatattagtt ttgcaatggg aaatagtcat caagaaatca
      841 tgaaaaaagc tgattatgtc actgtttcaa atgataaaga cggagtatct caagcatttg
      901 aaagaattct taccatataa actaattaag gtagtataga acatagaatt ctaaatacgg
      961 aaaaatggag cataaattcc agtacaaata gtacttcttc agtgtcaaac gaagcgaata
     1021 taacatagat atttaggatt gtgacaaaaa tcatgtcaca atcttttttt cgaataaact
     1081 tattttttga agtcggccat ttatcgtaat ttctttgcat aatacgaaca aattttcggt
     1141 tattgatgaa aaaaaacatt gaaaatggta tgataaaaga aaaaaaggag gaaattttta
     1201 atggtgaaat ttatccatgc ggcagattta catatggatc ggtcatttga aggactgaca
     1261 actcttgata aaactgtaca ggaaaaacta ttgaagacga atctgaccgt tttatcaaat
     1321 atcattgaac aggcaataat aaataacgtt gattttgttt tacttgcagg agataatttt
     1381 catcaaaatc gtccttcatt aaaaatacag aaacattttt cagaacagat gaaacggtta
     1441 gaaaagaacc atatacctgt atttataata ttcggaaatc atgatttcta tcaaaaagag
     1501 cggtattggt tctcctttcc taaaaatgtc catttattta catccgaaac ggtagagaca
     1561 aaaaaaataa cgataaaaag tggagaaact gtctctctca gtggatttag ttatcgtcaa
     1621 ccgtggattc aaaaagacaa agtgatggaa tttccttcta gagaactcac agattaccat
     1681 attgggttgt accacgggga acctggcgtc tctcaaaaag gaaactatgc tcctttccaa
     1741 ccctcaaaga tgcaagaaaa gggatacgat tattgggctt tagggcatat ccatgtgcca
     1801 acggtcttaa atgaaaagca aacaattgtt tatccaggtg cgcctcaagg tcatactaaa
     1861 aaggaacaag cttctacatc tattctacta gttgaattaa gtaaaggaag ttgccaaata
     1921 catccgatta aagtagcaga ggtctattgg aagacaaagg aaatatcatt gaggaaagca
     1981 agaacgacaa aagaggtcat tgttcacatt cgtcagcagc tttcaaaagt agaggatgga
     2041 tttagtttaa ttgagataaa attaaaagat tatgaccatc tcaatacaga tgttttggaa
     2101 cggatacaat caggagagct attagactat ttgttagagg aattctcaga taggataaat
     2161 gatttcttta tttggcgtat ttctctcata gagaatactt tttatacgaa gaccccactt
     2221 gctgcttctg ctaaattgat ggaacagtta tttcaatact atcagacacc tcaagatttc
     2281 caacaaattt taaatgaagt atatagtcat caagaagcgg ctagaatatt gagcgaactg
     2341 ccggaatatc aagaagaaac acttgaaaaa gccaagcagc tgcttaatca agatttttta
     2401 tttgaggagg atcaggaatg aagattttaa ggatcgagat tgctgctttt ggaaaatggc
     2461 gtcaaaaaag ttttgacttt tattcaggca atcagctgat ctatggggga aacgaagcag
     2521 gaaaatcaac gatttatcaa tttattcaag caatattatt tggtttccct tcaaaaggcc
     2581 gtaaaaaaaa ggattataca ccaaaagacg gctcagccta tggcggcaaa atatggctta
     2641 aacatcctgt atatggtgaa tttgctgtcg agcgctacaa gcagcaaaat cgcggaaaat
     2701 cgaaggtgtg gctgggggat caagtgggaa gtgatgagtt actagaaaaa ctgatcgcac
     2761 ctttgacgaa agagttattc cagcaggtat ttactttcca gcaagagcag ttgatggaac
     2821 tagatcattt gagagaaaaa gagctacatg atgcgctgat ttctttaggc atcacgggca
     2881 gttctcaagt attccaaaag cggcaagaat attatcagaa agcacaacaa ttattcaaga
     2941 aaaaaggcca aaaattacta atcaatcaaa aattaaatga atggaaagag ctgcaagaaa
     3001 aaattcatca aaaacaagaa caggaagctt attttggcaa gttgatccaa caagaacagg
     3061 aatacgagtc gcaacagcag cagttattgg cacaattaaa agaggcgaac aaacaatttt
     3121 cattagccag acagcaagaa cttaattttt ctttatatga agaatggcaa tctctcaaaa
     3181 aaaacagcac tacaggtctt cctgatgagc aacttctttt agatctggag tctttctcca
     3241 aacggtatca gcaaacaaat gagcgtttgg aagaattaga agcaacactg gaaaagcata
     3301 gcgggatgga tcagcagtca gccagatatt atttttattt agagcaggag caggaactaa
     3361 aagatctcca gcaacttcaa tttacagcgg aacaattaaa cgaagaatgg caaagattac
     3421 aaattgagac tagagaaaca aattcagaat tgaagatttt ggaacaaagg tggcattggc
     3481 agaaaaatcc gttaccggaa gaatttttat atgaagaaga atggaagcat cttctggctg
     3541 aagaaagaag gattgctgat ctagaaagcc agatttctgt gcgtctccaa atagctaaag
     3601 aacagcaaga gatgattgaa gcagaagtga atgacttgga agagacctat ccagcattat
     3661 tacaaaatca gtcagagata gcaccagtct cttcatctaa accgtatatg ctgatggctg
     3721 gcggagcaat aatcattgca ggtatatttg taccaatgcc cttgaaagtg atccttttac
     3781 taattggtgc aggcatgatt ggctatttct tctacaaaag gcaaccaaaa attaaaacta
     3841 aaaatgattt ctctgagata aaaggaatgt ggcaagaaaa actaggacaa cttgatctag
     3901 ccttagctca attagctgaa gcagaagaaa aacaaaagag aactggagaa gaaaaacaga
     3961 ttcttcagca agaagtatat cgttttgcgg atcatcggca cttaggaaag atggatacgc
     4021 tcaaactcat gaaagaatat ggacaggaag tcatagatta tcagaaatta tatggcgtat
     4081 ttgagcggaa acataaaaga gaacaggaaa tacaagaaaa actacgacaa atcgaccaaa
     4141 aatttgagtt tgttaaagaa tggttaccga tacaagataa gacaatttca gaaaaaatgg
     4201 tattgctttc ccgttttata caagagatgg aacagatgaa atttgccaga agctatcagc
     4261 aaaatacttt attgaagcaa gaaatcaatc aattgaaaaa gaaacaaaaa gtatggatac
     4321 aagaatacag cgaacaattg actcaagcag gcttatctta tccttcggaa gtacctgtct
     4381 ttttacaaaa agccaaacaa gccgaacagc agatgaaaag atacaatgaa ttatcggaaa
     4441 tgcttactcc actatttcct gaagagctga caaaagagaa attatctgta agactgcgcc
     4501 aactgaaaga aaagcaatcc caactccagc aagaagaaaa agaagcaagt gaaaatcggc
     4561 aacgactcca acttcaagta gaagagttgc aaagagacgg cacattggac gaactttatc
     4621 aacaagctag ccggcaaaaa gcaaaattac aggaattact ggaggaatgg ggagcacttg
     4681 aaatcgccgg gacattctta ggtgatcttt caacagagat gagtgaaaga caattacccc
     4741 agttgttaag agcagcaggt gattatttag gtattttaac aaaaggaaat tatcagaaga
     4801 tattactgaa aaatgataca ctgactttaa cagacggata tacagaattt cccatctatg
     4861 agttatctac aggtacgaaa gatcagctga ttatggcgat gagatttgct tacctctcat
     4921 tagaagctaa ccgttcgata tgcccgatca tcattgatga tggctggctt cattatgatc
     4981 atcagcgaaa gtatcaatta gctgaacttt tagctaaatt tggagaaaag tatcaaataa
     5041 tttgtttttc ttctgataaa gaaatggtaa gctattacca tgagatgaac caaatcgtaa
     5101 aacaattaga aggagcaaat aatgaaaaaa attcgtgatt tagtaatcga tgagcagttt
     5161 gaacaatttg tcctgatcaa aagtgcagat gtaagaatcg ctaaaaacgg aaaaaaattt
     5221 attgctttta ctttccaaga tacttcaggt actatcgatg gtaaatactg ggatgcttcg
     5281 gaggaggaaa tcaataaatt tacagccggc acagtcgttt ttctaaacgg taaaagagag
     5341 gtttatcaag gaaaccctca agtaaagatt cttcatatgc gcttaacaag ggtagatgag
     5401 ccaggagagc ctagtttata catggaaaga gcccctttga agaaagaaga aatggaagaa
     5461 gaaatcaatc aattgttgtt tgagatcacc aatgcgcatt ggaatcgtat tgttcgctat
     5521 ctacttaata aatatcaaaa acaatttttt gagtttccgg cagcaaaaag aaaccatcat
     5581 gcatttgcca gtggattagc ttatcatact ttgacaatgc ttcacttagg gaaatcaatc
     5641 gccgatgaat atcctgagct gaatcgagca ttgttgtatg ctggtatcat tttgcatgat
     5701 ttaggaaagg tgattgaact ttccggtcct atgtcgacag aatatacgct tgcaggaaat
     5761 ttgatcggtc atctggtatt gattgacgaa gaaataacta aagcctgtct tgcgttgaaa
     5821 atcagcgaag ctgacgaaga tgttctcgta ttacgtcata tggttttagc acaccatggc
     5881 ttattagaat atggctcacc tgtacgtcca agaataatgg aagcagaaat attacatcaa
     5941 atcgacaata tcgatgcttc gatgcagatg atgttgactt cgattcgtca aacagaacca
     6001 ggaaaataca ccgatcggat tttcggtatg gacaaccgaa gtttctatgt accaaaagat
     6061 ttatagcaaa aatttatttt tttcattcaa aacaaaaaaa gcgagacata agtcttcgct
     6121 ttttttgttt tcttttattt tgatgaatca gttgtttctt cagttgaaga ttcttttgtt
     6181 gaatcagttg attttgtgtc agaactcttt gttgaagctg tagtttcact tgagtctttc
     6241 gtacttgatg aagttgttgt gaatgcactc aacacatttt caaaggcatc atctttgatt
     6301 tttacatttg catcttttaa ttcttcaccg attactttag ttgtgaatgc attgtcactt
     6361 agtttagttt cagtagcgat atcttttagt tgatctttgt atttatccat gtcattgcct
     6421 ttattttggt ttttcaccat tttgacaacg tagtattctg tcgcataaga agtaggattt
     6481 gttgtagtaa taacatcgga gatctcaccg tctttcaatt tgaaagctgc ttcttttact
     6541 tctgcaggaa tagttgttgt cgttgagtcg aatttaactt taccgccatc ttcttttgtc
     6601 tctgtatcag ttgatttgtc tttggctaat ttagagaaat catctccatc atccgctgat
     6661 tttttcacat cttttgcttc atcttcactt gataatttga tgatttgtgc ttctacttct
     6721 ggatggaaag atttccaagc agttttcaaa tcgtcatctg taatatctac gtgagctttc
     6781 aagccagctt caaaagctaa attgttacga atatattctt tataagtatc tttcgtatat
     6841 ccagcagctt ctaattgtga ttcaaaagta tcacctaatg atttcgcttg tttgtcgtat
     6901 tctgcatcca cttgcttatc ggttacttta tcgccatatt tgttattgaa tactttataa
     6961 atgatcatcc gttgtactaa ggattggttc gatgattcaa gttttgcttc atcatagaaa
     7021 tcagatacag tgatcgttcc gcctttcatc gttgcgatat ctttattagt atcgcctgaa
     7081 caagctgcta gagttaaaac agccaacgca ctagtagctg ccaaaataat tgattttttt
     7141 ttcattaaaa tttgcactcc tgtaattagt tattattttt atatattaac tataccatat
     7201 cccatttcct ttaaaaacgt tgtcgtggta gtttcaaaca aaattcaaaa tttcatcaca
     7261 ttcctaggaa atacgcaaac ttttctcact tatatagtta aatctgttga gaaaataatc
     7321 attattatat ttgttcagga tactctggca gatcagcttt tagtttttct acttgttcat
     7381 tgatttgtgt gatacgtggt tcggcttgga atttaaaatc ttcgatatct tttttgattg
     7441 cttcttggaa agcaggaatc acagattcag ctgtttcttt tgtttcgatc aatgcgcttt
     7501 taaaatgatt caggctgtta tttaaatcat ttgtcagagt tgttgcttct tccaattctt
     7561 cgatcagttt ctttcttgtt tcattcccgc tcctgggcgc caataacaga cctccgacag
     7621 tgccagcagc agctccaaat aacagtcctt tgaaaaagtt tttaatcatc gcaggacacc
     7681 tgctttacga ttgtttcttg aatagctttt agttcttccg atccataact atcttgatga
     7741 ttgacaaagt gaatagaaaa atcatcctct ttgctgtagc gagggactaa atggatatgt
     7801 gaatgaaaaa cagattgata ggctagttca cggttgttat tcaagatatt aagacctttc
     7861 atttctggaa aagctttttc caatgcttga gcaatcttag gcacacgcga aaaaacctct
     7921 cctgcaagtt cgggatcata ttcaaaaata tctgctacat gacgtttagg cacaactaat
     7981 gtgtgtcctt tcgttgcttg agagatgtct aaaaaagcat agaccttgtc gtcttcatat
     8041 attttatagc ttgggatttc ttgattgatg attttgcaaa aaatacagtt ttccatataa
     8101 aacatccttt ctataaagaa taaatacttc agcttaactt taccataagt taaagctatt
     8161 gtaaggaaaa aaacaaaata ccgtcatttt cttcgtggta taataaataa aaataaatgg
     8221 cggaggaaaa agaatgagtt taattatcga tcatgtcaca ggggggtacg gacatctccc
     8281 tgtgttaaaa gatatcagtt tcgatgtaca gcctggagag atagtagggt tgattggact
     8341 gaacggagca gggaaaagta cgaccatcaa gcacattatc ggtctgctgg aagcttcaaa
     8401 aggaacgata gaaatcaatc atctatcctt aaagaaaaat acggaagaat atcgcaaaca
     8461 aattgggtat attccagaga ctccttcttt atatgaagaa ttgacattga gagaacatat
     8521 cgaagtcact gcgatggcct acgatatacc tatggatata gcaatgaaaa gagcagaaaa
     8581 gttgttggag acttttcggt tatctaatcg tcttgactgg ttcccagcta atttttcaaa
     8641 gggaatgaaa cagaaagtga tgatcttatg cgcctttttg atcgagcctt ctttatatat
     8701 catcgacgaa ccatttttag gcttggatcc actagctatc aatgcattgc ttgaattgat
     8761 ggtagaaatg aaaaaagaag gagccggtat tttgatgtct acgcatattt tagctactgc
     8821 agaaaaatat tgtgatcgtt ttgtggtctt acataacgga gagatacgag cagacggttc
     8881 tcttgagatg ttgagaaaag aatttcagct gccggattct tctttggacg aaatctatat
     8941 tgctttaaca aaggagcagg cacatgagta gtttttttca aaaacgttta gcaagacatc
     9001 aaaagaaaat gatgaaatat atgcgttatg tattgaacga tcattttgtt ttagtttgtc
     9061 ttttcttgat tggcggtgtg ggattatatt attctaattg gttgaaaacc ctgcagccac
     9121 cgtttgcaat tggagggctc ataatctctg tattttggat gctctgttta ttcgtgggta
     9181 agatggctac ttttgcagag gcagcagata ttgttttttt acttcctaaa gaaaaagaaa
     9241 tgcaaaccta tttggaacga gcttttcgct attcttgcat ttttccgttg atctttttga
     9301 tattggcatg cggattggcc atgccattag tggtggttag tacggagcaa tccttttcga
     9361 tgtttttcgt ctatctgatc atattatgga gtttgaaatt cagtcatctg caaatcaaga
     9421 gagcagaatt gtttcgacta gatcaacaag ttattcgtta ttggaaatta ggctggtttt
     9481 tttcttctct gatctttttg tttctatcat tttattggct aatatgggca ggaatagcag
     9541 gtgcattagt acaggcaagt ctatattact ggtttttgtg gaagcaacaa gaaaaaggat
     9601 tggattggga gaaaatggtg catgtagaag aacataggtt gcatcagatc tatcaattta
     9661 tcaatttatt cacagatgtt cctgaaatta cggcaaagat caaaagaaga cgctatttgg
     9721 atcctatatt gactcatatt aaaagagaat caaaaaacac ttatctttac ttgtttgcaa
     9781 gaagattggc aagaggttct gattttagtg gtctttattt ccgattagta attataggtg
     9841 ggttattgat tgccggagta gtcgactttt attttagtct gattatcggt ttcttatttt
     9901 tatatttgat cggtttccaa ctgttacctt tagcgaacca gttccgctat atggttctcg
     9961 ttcagcttta tccaatccat aagaatcaga agaagcaggc gattcagtat ctactcacat
    10021 gggtattaat tatcgcgtcg gttatttttg gtttgattgc tgcagtcgta ttagatgggc
    10081 agcagcgatg ggttccgcta gcggcttatt tgattgttac tatgctcttt gttaaactat
    10141 atgtaccaac tcgtttgaag aagatgtcag actgaaaaca aagctgtgta gggtaaaaat
    10201 gagaatgaaa aatagagcgt tgacagtaag aatgctgttc gttctatttt ttttgagaaa
    10261 agctggtttt ttgaatggca attaaatgaa aaagaacgta tatgtgcttg acgtgtagtg
    10321 ttcaggcgac tagaataata agtactgcaa aacaaagagt aagaggacgg gagaatgcat
    10381 gatggagttt cagttagacc aagagtggcg gttacgtccc atcaaaggcg acacaggaaa
    10441 aacatacata ggcatgcgtg atgaggacaa agtctttatt aaacgaaaca cgactccgat
    10501 gttggctgct ctttcaaggg aaggaattac accaaaactt gtttggacaa aacgaacggg
    10561 gaatggcgat actttgacag cacaagagtg gctggaaggt cgcttgctga ctcctaatga
    10621 gattggtcaa agaaacgacg tgattgatgt tttgtaccat ttacatcatt caaattctct
    10681 taaaaccatg ctgacccgta tcggtggtcg tgtcatgacg ccggagaaga tgctgcaaaa
    10741 atatgaaaaa gaattaccgg aagaactcca gaaaaatcaa tttctgcaaa ttgtccatcg
    10801 ttttcttcgt gagcagatcc cgtcaataga taaggaaaac ttatcagtag tacacggaga
    10861 tgtaaaccat cgtaattggt tagtatgtaa aaattattta tatctagtcg attgggattc
    10921 aatcatgttt gcagaccctg cggtggatat tgcaacgatt ttaggaaatt atgtgccact
    10981 gtctagctgg aatcagtggc tgatcagtta tggcatacgc ccgactgacg aaatgcttga
    11041 aaaatttcat tggtatgcgg ccatgaattt gctccaagaa atcaccagat attgcaaacg
    11101 tgaagatcat cgacggatga acgaagaaat tttacaatta aaacgaattt tcagtggctg
    11161 aggtttggcc caaactctgc ggagatttgg ccagaccttt ttaattagag gaaaggaatg
    11221 gagataaata atgcgtgtcc gaaatcgtcc gggagcagcg gaaatgttag cggctcaccc
    11281 gaattttgta attagtgatc caacattgtg gaagggaaaa tggaatgaat tgtttgaaaa
    11341 cgatcatcca atccacattg agattggtat ggggaaaggc cagttcatca ctggaatggc
    11401 aaaagcccat cctgaaatca attatattgg agtagaaatg caggtcagtg ttgtttcgat
    11461 tgcactagac aagttgatcg aacaaccgct tcctaacctg aaacttctac atgtggatgg
    11521 atcagcgttg acagagtatt tcgctgacag cgaagtagat caaatctatc tgaatttttc
    11581 cgatccatgg ccaaaaaaac gtcatgaaaa acggcgattg acatataaga cttttcttgc
    11641 tgtagatgaa cagattcttc gtccaaatgg agaaatccat ttcaaaacag ataatcaagg
    11701 attgtttgag tattctctgg caagtttttc tcaatatggc atgatcctca aacaggtatg
    11761 gttagattta catcaaagtc aatttgaagg caatattatg acggagtatg aagaaaaatt
    11821 ttcttcaaaa ggtcaacgta tttatcgagt agaagctcaa tttcaagata agaccaaaaa
    11881 gaaaagtctg tgaaactaac tttctgcttt cataaaaaca ttaatatcca taaataaatc
    11941 ccagaaaatg aacattacag atgtaatgta ttttctggga tttatttgat ttaaaatgta
    12001 tttatttcat cggtttttcc acaaggtgga atcaattgga agcgagaaaa tgaaaataat
    12061 cctttgtcgg tgatcttcaa ttcgggtatg acagataatg ttaagaaaga aagtgtcaaa
    12121 aaaggatcaa aggttttagc tgctcctagt tgatgtgctt tagttattaa ttcattcaac
    12181 tgttcataga cgatcgaaaa aggttcctgt gtcatgattc cgcctatagg aagagaaaca
    12241 caagctattt cctgctgttt gttgacaacg gtcatcccgc cacctttttg aatcaaaaga
    12301 ttagcagcat acagcatatc ctcgtcgttt gtcccaacga caactagatt atgcgagtca
    12361 tgggcaatcg ttgtcgctat cgctccttgt ttcagctgaa atcctttcac tatgcctaat
    12421 ccaatgtttc ccgtttgatg gtgacgctcg atcaccgcaa tctttaattg gtctttttcg
    12481 attgaaggaa tgaatcgttc gccttgtcgg tccacttttt ctaccaaatc tttagtcact
    12541 agactattcg ggataatttc gatcacatgg gcttgtgaag aagaaagaag cagctctaac
    12601 gaatgtggat ttaatggttg agcgtttagt tttggcagac aacacgtata tgaatttgaa
    12661 tgatcagcaa ataaatggtc ttggagttgc ccagattgaa caactaatgt tcccttatag
    12721 aaaacttcat gaattggcag cttggtaaga tcgtctgtta agaagaaatc agcttgatac
    12781 ccaggggcaa tcgcacctag tgttttcaaa tcaaaacatt ctgctgcatt caatgttgcc
    12841 atctgaatgg cttgtaatgg atcgattcca agtgcaatag cttgacggac gatatgatcg
    12901 attgatcctt cttttactaa atcattgatc agtttatcat cggtgacaaa gaagcatctt
    12961 cttgaatttt caggtgtgat cgccggtagt agcgatgcca gatcttttgc aacggtccct
    13021 tctcgaacca ttaaatacat accaagatcc aaacgttctt tagcttcttt tgcagttgta
    13081 gcttcatgat ccgtccgaat acctgctgca ggataaacat ttaactcttc catgccgata
    13141 ccagctgcat gcccgtcaat ttttttctta tgctggtgca tcaattggat cttttctagg
    13201 atgtctgttt cattatttgc tactgcttga tagttcatga cttcagctaa gcctaaaact
    13261 tcgggttttt gtaaaaaagg ggctaatttt tttgcattta aatttgcacc attggtctca
    13321 aaaggggtta caggtacaca agaaggtagc ataaaaaaga tattcatgag tgtttgtctt
    13381 gcatcttcta tcataaactc aagacctgta ctcccagcaa tgtttcctat ttcatgggga
    13441 tctgtcacta cagtagtaac tccatgaagc aaggatactt ttgaaaattc gcttggtgat
    13501 aaaagtgagc tttcgatatg cacatggcca tcgatgaatc ctggcactag atattgccca
    13561 ttagcattat gtacgctttt tccttcgtat tcaccgattc ctacgatcat tccttcgcag
    13621 attgctacat ctttttccat gatttctttc gtaaagacgt tgacgatctt agcatgcttg
    13681 atcactaggt cagcaggctt ttttttactt gctacttcga tatatttcct taatgttgtt
    13741 tccaatggag tgcctcccaa gataaatttt tataagtata acataaattc ggtttttgcc
    13801 aatatttttt tgtaaattca ttttatttga aacaaaaaga tcaaaaagac gtatgttttt
    13861 tgtggagggg agaataatgg aaataaaacg attatcggat caaatgataa gtgaaagttt
    13921 tgccaaacat gcccaagacg tatatatatt gccagcttca gaagtatttt acatctattt
    13981 tcgctatgga aaaaaacgaa cattctggaa gaaagtctct tcggcagatg gacagcaact
    14041 gatctcaaga tttaagtatt tgggacagat ggatatcgga gaaaaaagaa aagctcagtt
    14101 aggtgcattt acttattctt tagaaaaaca ggaagttcgt ctgcggttgt cttctgtagg
    14161 agattacctt caacgagaga gtttagtgat tcggctatta tacggtactt ttgaaacacc
    14221 ccatcgttgc tttagttctc gagattttgc aactatttta cagaaaaccg tcagacgtgg
    14281 attatacctt ttcagcggac cagtcggatc aggcaagact tctctaatgt atcggttggc
    14341 tttggaagaa aaattgcaag tgattactat cgaagatcct gtagagatag aagaaccgtt
    14401 ttttctgcag ctacagatca atccgaaaat agcacagact tatgatacac ttttgagact
    14461 cgcattacgg catcgtccag acttgctaat cattggcgag ataagagatg aatcaactgc
    14521 acatgctgcg gtaagagcag cactaacagg gcaccgggtt tttgcaaccg tacatgcacg
    14581 caatcttgaa ggtacattgg tcagaatcca tgaactgaca aagaaaaaag aagagctatc
    14641 tgaatgttta gctggtgtca tttatcaaga acttttggaa gatgccaatc accatccttc
    14701 tgttttatgg agttatcaat ttatggagga agaatcagta aaaaaatcat gggaggaaag
    14761 ttatgaagaa gctcaggaaa atatttggca gtcagaacta ttctaaagga ttaacaagaa
    14821 gccagcaaaa ccagtttgca caaaccatgg gagaactgct tttatctggt tttagtctgc
    14881 aagaatccct ttatttatta ctcacgatcc gttcgtttcc acctgttatt ttggcaaata
    14941 tccaacatca gctaaaggaa gggcaagcgt ttagtgaggt actttcgcaa ataggatatg
    15001 gagcagagca actggtacag atcgagttag ctgaacgtca tgggaatctg ccgacaactt
    15061 tgttggaaat tgccaaacag tttcggcttg ttgaaggatt tcgcaatgat ttgaaaaagc
    15121 tgcttgcata tccgatgcta ttgctcacct ttttgctagg tatcttgttt agtatgcggt
    15181 tgttcgtttt gcctcagctt attggttcag gaatggtcat gcaagagcat tggggtattc
    15241 agctgataca agtctttcat tggtatgttc tactattcat cctagcggtt actctattgg
    15301 ctttactagt aaggtatcgt ctgatgaagt tgtctcctgt tgatcagagt gaatggataa
    15361 gcagacaatt attgattgga ccgatatatt ccttgtatca atcggcttat ttgtcactag
    15421 agattggaaa gctttttcat gaaggtttcg aattaaggca aatcatagtc tgcttaaagg
    15481 aaacgaaaca agaaagtctg attcagcatc ttgcctctaa gttgattcaa ggattggagg
    15541 caggtatgtc attggctgag cagttccaga attatcgttt tctatcagaa gaattcagta
    15601 aaattgtttt acagggagag gcaaagggaa gtttaggtaa agaactgatt ttttacagcg
    15661 aatggacaag aaaacagctt ttcaaacgtc ttcatcactg gatgcatttt attcagccaa
    15721 tcattttttt gggcgtagcg gctttgattt tattagttta tgccgctgtt ttattacctg
    15781 tttacggaag catagaggag gttttgccat gaaaaagaga aaaatatctt attatagcgg
    15841 ttttacattg atcgaaatgc taatcgtctt actgatcatc agtgtattag tgttgttgtt
    15901 cgtccctaat ttgtcgcgtt ataggaatca tgtagaccaa gaaagtcgtg aagcgatcat
    15961 ccagttggta gacacacaaa aagagctcta tgcgcttcaa aataatggaa gagttcctac
    16021 tgtggaggaa ttgttgaatg aaggctatat caaacgagaa catgcagaaa tctatcagcg
    16081 gccttgaagg taaggtttta gcatcaggat atacgttgtt tgaatcactc ttactgattt
    16141 tattacttac aacgatcctt tcttttccag ttctcgcatt ttctgcatgg aagaatgaat
    16201 tggcagtcta tcaatttttc ggccaatttg aaaaacgaat ctattctacg caaaaaatcg
    16261 ctattgtgaa tcagatccaa acaggctttt attggaatag tgaagagaat caaatcattt
    16321 ttcatgttcc gaatccagat aaaataaatt gggaagtatt agatattcct gatgagatta
    16381 ccttgaaacg ccatgcttct attacttttg ctgctagaac cgggaatgaa agttcactaa
    16441 aagcttatca attttattgg gaagaaaaag agcggacgat tacgtatcaa ttccagatag
    16501 ggagtggtcg ctatacaaaa agaattgaat aaaacaggat ttgttttgtt ggagtccctt
    16561 acagcgctta tgatcgcttc tgtctgtgtc tacttagtga gctttggcca gctgtttttg
    16621 cttcaggcag aaaagaaaag tggggaggaa ctgctattta ttcgaatgat ttatgaagat
    16681 atcaagacgc agcggatgta tcacaaaaga ccagagcgaa gggaagaaat gatcccatac
    16741 gatatccaaa ttgtcgataa taatggagta atcgaaaaag tgtttgtcaa tatggagaaa
    16801 gaagcgatca gtattgaaaa aacaagtaat tccagcattt acgctgattg aatgtctaat
    16861 tggactgatg atattgagca gtctattgct cacattcaat cttttgattc agcaaaccat
    16921 ccaagtaagt gaatttttgc agcgtaaaga tcaaaaagag tggctaatat ttttagcaca
    16981 attagaagaa gaactcaaga gcagtcacaa tgtctctgtg aaaaatcagc aactgcacta
    17041 cacgatcgag gataaacagt atatcatcga aagatatcag gcaatgatac gtaaaagaag
    17101 aacttccggc ggacatcaac ctatgttaac aagtatcact gagcttcaat tattggaaaa
    17161 agaacacaca atttcttttt atgtacactt tgaaaatgga gaagatggat atgccatatg
    17221 gacaaaaaac gatcagtaga aaatgttata agggctctat tttattttct gtcttgcttt
    17281 tatttctttt atgtacgttt ttattgatgg cagcattaga aagctaccgt ttgtcaatgg
    17341 atcttaatat acgaatcaaa aattattata tagcaaaaat aatgaagcag atggctttat
    17401 cggaacagtc tacgcttgcc gaaagttcgg aaggagtttt ttactacaca actggatctg
    17461 taacatatca gtgggtacaa cagtccctat tcttagaggt ggaagtctca ccgtttatct
    17521 ttcggttcac agaagaagta aaaaatgata cagatactag tacagaatga tggaatgttc
    17581 gtgaaaacgc cctcttttct gttataacac tgtttttaat aaaaaaatgt ttgccgtttc
    17641 acaaataaaa aacagtgaaa acgattgaaa ttttgtcaat tgttcgatag aattaaacag
    17701 gtgaaaaaat tgttataata tatctagaaa tgaggtgcac catgttacca gaaaaaatgg
    17761 aacaagggtt taatcaaaac ctggaagcta ttcagctttt acagaatgca ttgggaactt
    17821 cctttttaga agcctatgtc gaaaatgcag aaaatttaat agatgattat caagtacgtg
    17881 tggtggatgg agtgccgaca aaagagacaa cgcaacggat cacagctctt tatgaagagt
    17941 tgaagaaact ttctttcgag ccagaagaat ggcgcagact gtctcaatta cttttattga
    18001 agggcagcca gacagaacat ttgcaggcga accatcaact cactcctgat agtatcggtt
    18061 ttttatttgt ttttcttatt gaacaactat acacaaataa aaaagcaccg gttaagattc
    18121 tggatattgc ggcaggaatg ggcaacttgt tattaacagt tcttttaaac ctatcgaatg
    18181 cggggtacca aacagaagga attggtgtgg atattgatga tacattgttg gctgtcgcag
    18241 cttctacaag tgatttgaca caagcaaatg ttcaatactt tcatcaagac gggttgcagg
    18301 aattattaat agatcctgtc gattttgcaa tcagcgattt gccaatcggt tattatccga
    18361 atgacgaaaa agcaaaagag tttttaacaa gtaccgaaga aggccatagt tatgcacatc
    18421 atttattgct agagcaatca atgaaatatg tgaagccgga tggttttggt ttgtttttga
    18481 tgccgagtgg atttttagaa acggatcaaa gtgaagaaat caaaaaatgg tttaaagaag
    18541 aaggttatct tcaaggaatg attcaattgc cagatgaatt gttccgtaat aaacaatcgc
    18601 aaaaaagtat tttgatctta cagaaaaaag gaccacaagc acaacaagta aaagaagtct
    18661 tgcttgtgaa acttgcttca ttaaaagaac cagagaaggt gacggaattt ttcaacgagt
    18721 ttaaaaactg gaaatcatct gctctgtaat cattcaacga gaaggagagt catatgtcta
    18781 aaacaattgc aatcaatgct gggagttcaa gtttgaaatg gcaattatat caaatgccag
    18841 aagaaaccgt agttgcgaaa gggatcgtcg aacgtatcgg tttacaagat tctattttta
    18901 ccatcaagta cggcgataat caaaaattcg aacaaatcat ggatatcgaa aaccacgaag
    18961 tagcagtaaa aatgctgtta gatcagttga ttgaactgca aattttaagc tcttatgatg
    19021 aaatcaccgg agtagggcac cgtgttgttc acggaggaga aacttatggg gattcagtag
    19081 tgatcgatga gaaagtaatg gaccgcattg ctgaattagc agaatttgct cctttgcata
    19141 atcctgcaaa cttaatgggg atcaaagcgt ttaaaaagat tttacctgac attttgagtg
    19201 tggctgtatt cgatacatct ttccactcaa caatgccaaa gcaaaattat ttatatagct
    19261 taccggtgtc ttattatgaa gatttcaaag taagaaaata tggcttccat ggcacaagtc
    19321 atcgttatgt gtcggaacgt gcggcaaaat tattgaacaa accgatcgaa gaactaaaaa
    19381 tcatcacttg ccacttagga aatggtgtat cgatcacagc agtcgatgga ggtaaatcca
    19441 tggatacttc tatgggattc acaccattag ctggggtgac aatgggtaca cgttcaggtg
    19501 atatcgatcc agctgttctc ccatatttaa tggaaaaatt agagattgat attgatgaaa
    19561 tgatcaatgt tttgaacaag aaatctggtc tgctaggcct aacaggattg tctagtgata
    19621 tgcgtgatct tgaaaagaac tatgaaaaag aacatattcg tttagcttat gacattttcg
    19681 tggaccgcat ccgaaaatac atcggcggtt atgtgacaac gatgaatggt gttgatgcaa
    19741 ttgtctttac tgctggtatc ggcgagaatg acggacatgt acgtagtgaa atcatcaaag
    19801 gtatgtcttg gtttggttgt gaaatcgatc cagcgttgaa tgaattgcgt ggtgaagaag
    19861 tagatatctc tacaaaagaa tcaaaagtaa gagtattagt gatcccaaca gatgaggaac
    19921 taatgattgc ccgtgatgtg gagcgtctgc gcggctaagc caaagcattc gttaggataa
    19981 aagatgaaac gcatgcaaga gggaattctt gcatgtgttt tttttgcgga gaagaccgca
    20041 aaatggtaat ttaacttcaa tttcccttct tttttacagg atcttcttag ggaattttgc
    20101 ttggaaaagt ggtcaaaagg gaaaagactg gcattttatt tattgatttt caggaaaatc
    20161 ccaatatttt attttcattc atctttataa gggacattac atatgtaaaa agaaaaaaca
    20221 tttttagatc agcctcgtgg gctgtcgact ttattcttcg ctttatgttt agctagaaaa
    20281 ccgatccgaa acattcattg atcgaataat aaaaacttga ttttccacct gtactttgcc
    20341 tgaatcgtga aagcacaggt atgaaaatca agttttttgt tgcgaaaaaa cgtcctagtc
    20401 aatcagttgg ccgagcggct ggcggctgat ctgctcctga tcaataacga gtgcttcttg
    20461 gaagtgttgc tgcaaaaagt agcggtcatg gctgactgct aggagcgttc cttgatagtc
    20521 ttgcagcatt tcttcaattt cttctcgagc atagctgtcg aggtgattgg ttggttcatc
    20581 caaaatcagg acgttgattt tttctaaaaa gagtttcagc aacgccaaac ggattttttc
    20641 accaccggat aaatctttga tccgtttacc cacatcgctt tgataaaagc cacttttggc
    20701 taattgccgt cgagcgactt cttcgtttcc agtcaatgtg atggcgtaag ccagcaaacg
    20761 gctatcggga tcggcaaact gcagctgctg cggcaaataa ccgatttgca agctactacc
    20821 taatttgatg gtgccaccgt aaggcttttc ctcaccgagt aataatttga gtaaggttga
    20881 ttttcctgca ccattgtcgc cgatgatggc taaatgatct ccacgataga ctgcaaaact
    20941 gctgtctttg aatagtactt gatcagcgta ctgctggtgc aagttttgaa tgatgaaaac
    21001 ttctttgcca gattttccac cattagacaa gctttgcagc cgatttttcg ggggaggtgg
    21061 cggtttgacg agtgtcagtt tcgccaggcg gcgttccagt tctttggctt ttttgaagaa
    21121 ggactcattg tctccttcat gtgcccactg acgaaaacga cgaatcatga ctttcaaccg
    21181 ctggacttct ttttgctgca gttcgtaatt ttttgtcagc tcagccaagc gagcttcttt
    21241 caagactcga aaacggctgt agtttccggg atactcaatc aatgcacctt cttccagttc
    21301 ccatgttttt gtggtgactt gatcgagaaa ttgccgatca tgagaaacga ccagaaccga
    21361 tttttttgta ttctgtaaat agttttccaa ccagcgaata ctgttaagat ctaaatgatt
    21421 ggtcggttca tcgagtaaaa gtaaatcggc atcggctgct aaaattttgg ctaaagcgac
    21481 tttaactcgt tcgcctccgc taagttctgt taaccgtgct tcctgacggt gtggaatccc
    21541 taagccttgc aagatcgctg caatccggtc ttctaatacg taaccgccta gttcttcaaa
    21601 gcgatgctgc agctcaccgt aacgggccag acacttctct aagtttgtat ctggttctgt
    21661 catcaatgct tcgtagcgac tcatttgctg tttgaggtcc tgtatctcag cggcagaatg
    21721 gagcaagtag tggttgacgg tcacttcgga aactgtaagt tcttgttcga ccgtcccgat
    21781 ctttaagcct tttttgcgac tgataacacc gctgtcgaca ccttctctgc cggtcaagat
    21841 ttgtaaaaag gttgatttgc cagttccgtt gataccgact aaggcaattt tttcaccagc
    21901 ttcaatttga caggataaat cctcaaataa tggattcccg ccgattgttt ttttaataga
    21961 aattgcttgt atgatcatga tctctctcct tgagaccact caaaaaggcc atggttatcc
    22021 atggcctgtg ttatttaaac tcagattatc atgagaaaaa taccttgaga tggccattga
    22081 ttttacgtga attcaactgt tttggacaaa cttgtcccgt caacagcaac tgtaaaacca
    22141 attgcatggc taaatgcgat atggtatcgt tggtaatttt cattacttgt tattttagtc
    22201 atgcgcatca tagtattttc cctccttcgt gtagttgtct tttccagtgt acctgaaagt
    22261 gtcagaaagt tcaactagcg ctgacattta cttaatattt atcgatgtgt aattctgtca
    22321 gagagctttt tttatctgac tgagggtaat gtctcgtgga ccacatcatt gtattctgag
    22381 ctaaaccagc gttcaggcga aatcatatgg tgtttgataa aaaactttgt ctacatgact
    22441 tccttttgcc atttgcttaa taccgttgca ttaaacagct cttctctttg gaaatctcac
    22501 caacatcgcg ttcaggagta tcgttgtggt tgcaatggca agcggtagcc tttcatcaat
    22561 tcttgaatga tttaccgaat gtagcacttg ccttctcgcg gtacccgaac ccgaaataaa
    22621 attttagttt ggcgataaaa tcggtatccg ccatcgatac ttcatatttt ttcgtatcag
    22681 attcatcgga aacttccaca ttgacgaacc aataagcttt ggcatgctta ggtcgtttta
    22741 aaccacacca gggcgactaa tcgccctggt gttcactttt ttttatttta cttgaactca
    22801 ttgactgttt tccctttatt ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt
    22861 tgtttttatc acggtttttt gtgcaaaaaa acctgccgat agttggcagg tcgtctacgt
    22921 gttaagcttt ggtgtggtct ttagctggca attggttttc taaatcagtt cggacaacaa
    22981 gcacatcgca agctgcgttt ctaataacat attcagaaac agaaccaata aataaacgtt
    23041 ctacagcatt caatcctgtg gctcctaaca taatcaaatc tacttgatta tcttctggga
    23101 tttctctggc aataatttgt tttggtgagc catattctac gacagaggtt acattattca
    23161 agccagcttt cttcgcattg ctttcataat ctgccaatgt ttgctttgcc atttcagtag
    23221 cttgttctgc aagcattcca tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga
    23281 catgtgctaa tagaagttct ccgttatttc ggatagctac attgaccgcc tttttaaaag
    23341 ctaattcagc ttcatcagaa ccatctacgg caaccatgat ttttttgtat tgttgtaaca
    23401 taagcattca ctcctcaagt caatcgtatt gtactctttt attgtagaac actttaggga
    23461 aaaagagaat tatttactgc tttggttcag caattttttt tgcaaatcgg atactgaagg
    23521 tggcagaaac aataattacg atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa
    23581 aagctgttaa aatacccatt acgcctaaaa cagcatatgc agctccgtaa aattgtagga
    23641 atccttgatt tttatcattt ttgttgatca atacaaaaaa gatatcctgt ttattaaata
    23701 aataatagct aacgaataat aatactaaag tggcgataat cattaaaatc ctaatcatga
    23761 acaaatcctc caaaataagc aacaaataga aaaagcaatt tgccaagagc aaattgcttt
    23821 attctttaaa agatggtaat ctccgttaat gccgttgttt gtcctgtagt attgatcccg
    23881 cgaattcaag caggtgggtc ccatggtcgt agcttcgaac taccaaatga taaggcatgt
    23941 taccagcagt gacacaagac gggtcccaag atatcaataa aaatgttcgg tcaacacaca
    24001 aagaggacaa ttcgaacatc acagatttcc cacctctata gtagcatatg aatttaaaaa
    24061 gagcaatgag agagctcgca gaaacagaac cgttttcttg tctagttttt tggaggatgc
    24121 tttttccact cttttatacg gtaatactgt tgacccagag cctgttcata tttccctgta
    24181 tcttttggct gataatactg agcactcttc agtttatctg gtaaatattg ctgattgacc
    24241 caagcctgat caaaatgatg aggatactga taacccacgc cacggttgag tgatttcgca
    24301 cctttgtaat ggctatctct caaatgatca ggaacatctc cagctttgcc ctctcgaata
    24361 tcagcaagtg ctgcatctaa tgccatgtag gcagaattgg attttggcga caagcaaaga
    24421 tcgacgacga cgtctgctag tgggatacgt gcctcaggca atcccaattt ttcagcagct
    24481 aatacagcgt tcactgtacg agccgcagca gcgggattac ctaaacctat atcttcataa
    24541 ccgataacca tcagacgccg gcaaatactt gccagatcac ctgcctcaac taaacgggcg
    24601 agataatgaa gtgcagcatc cacatcactt ccacgaatcg atttttgaaa agccgaaata
    24661 acgtcataat gagcatctcc atttttgtca tgggtaagag cttttctttg gacacattct
    24721 tcaatgatcg acagtgtaag atgaatcctt ccttctttat ctgaaggagt cgatagtgta
    24781 gccagttcca gcccattcaa tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga
    24841 agaagagcat cttcgtctaa ctggattgct tgttgtccaa ggccgcgttc tttatccctt
    24901 aaagcatgtt cgactgccaa ctggatgtct tgttctgtta atggtttgac ttcaaagatc
    24961 tgtgtacggc tgcggatcgc tggattgatg gtaatataag gattctcagt agtcgctccg
    25021 atcaagatga ttcggccact ttctaaatga ggaagcagaa aatcctgttt tgttttatct
    25081 aatcggtgta cttcatccaa taagaggatc actgtaccac tcatttttgc ttcttccgct
    25141 acaacttgta gatccttttt tgaatctgta gcggcgttta gcatgcgaaa tgcataatta
    25201 gtagagccgg cgatcgcact tgcgatactc gtttttccag taccaggagg tccatataga
    25261 atcatggaag agagcatgcg agcatcgacc atgcggcgta tgatttttcc ttccccgact
    25321 agatgctgct gaccaacgac ttcatcgatc gttcgaggtc tcatgcgata agccaatggc
    25381 tgttgcatag gattttcctc cttcttgttt ttacgtccct taattatatc atatttagca
    25441 agatgcctag aaagaacagc cttctgttgt tttgttttca tagcttatcc aactaatatt
    25501 gtgtatgata aagtaggtga gaaaaatgac ggataaacaa aagatagatg aatttttttg
    25561 gaataaaacg acgccagaag tggcacggga tttattgggg atgtatttgg agcatgacac
    25621 accagaagga agattagctg gatatattgt agacgcggaa gcatacctag gtccagaaga
    25681 cgaggccgcc catagctatg gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa
    25741 accaggtacg atctaccttt acacgatgca tactcatcgg attttgaaca tcattactca
    25801 gccagaaggg attcctcaag gagtaatgat acgtgcaatc gaacccgccg caatgattga
    25861 tcaaatgagt aaaaatcgtg gcggaaaaac tggtccagat atcagcaacg gtccgggaaa
    25921 attagtggaa gcattagcaa ttcctcaaga actttatggt cagtctatag cagatagttc
    25981 gttacgctta gtttttgaga aaaaaaagac accaaaaaag atcaatgctt tgcctagaat
    26041 cggtatccca aacaaaggtg tatggacaga aaagccgttg cgttttgttg tgtcgggaaa
    26101 tccttatatc tctttacaaa gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga
    26161 aaatgaaaaa gaaggatcta ttaacatatt tagatgaaat aattgaaaaa caagtgacag
    26221 actatgatgt agctttagat tgggatacaa aaaatcatac gatcgagatc gttgttcgct
    26281 tgtttgcaga aaataaagca caatttgaga ttgatgatgc agaagggatc gtttcgcaag
    26341 aaccaattat cgaatttgaa gacggcgtac ttctttttaa tcctcagaaa tctgtttttg
    26401 atgaacgcaa ttatctagca gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag
    26461 tagctgattc tttagtggaa tatctaaatg aagtattagc tcaagggcaa agcgatttgc
    26521 ttgatttttt ggatgaagac gatgacgaag ctgtattcga gcttcactgg gataatcagc
    26581 aactagcaga attagtagaa gtaaaacagc aaaatgaagc agatacttat ttaccgtacc
    26641 caagttatta aggagtgaca gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg
    26701 aagcagtcga agcagttgct aatattttga tggaggcagg ggcgagtggt gtcgcgattg
    26761 aagattcttt agatgttgaa cattttcagt cggatcaata tggtgagatt ttagataaaa
    26821 atgattttac atcgctgaaa gaaggcgcac tagtcatggc ttattttccg gaaacgactt
    26881 tcttaccgga aatcttgcct tttatcaaag atcgtatcac acaattacct gagtttggtc
    26941 tagctattgg aaaaaataaa gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag
    27001 cctggaagaa atactatcat ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct
    27061 gggaaaacta ccagacagaa gatcctctag aaaaaattat cacactagat cctggtatgg
    27121 catttggcac aggcactcat cccacgactt ccttgacatt gacagcacta gaaacagtct
    27181 tgcgtggtgg tgaaacattg ctagatgtgg gaactgggtc gggcgtgcta agtattgcag
    27241 caaaatatct tggtgcaaaa gatgtctatg cctttgattt agacgaagta gccgttcgtt
    27301 cggcgaaaga aaatatggat atgaatgaaa tagccaaaga tgtccatgta tcagctaacg
    27361 atcttttgac aggaatcgag attgaaagtg atgtaatcgt tgcgaacatt ttagcagata
    27421 tcatcttatt gatgatccca gatgcttggc gtttactgaa gcaaacaggc acgctgatcg
    27481 tttcaggaat cattgaagag aagaagcaaa tggttctgga tgccatgtta gaacaagggt
    27541 ttatcgtgga tcaaattttc cagcaaaaag actggtatgc aattattttg aaaaaaccag
    27601 aggtggaata gatgcagcgg tattttttaa aagaatcata tgaatcaaaa gactattttg
    27661 ttttagaagg ggaaaactat caccacattg tacgggttat gaggatggaa cccgaggatg
    27721 aagttttttt ggcattcaat gatcaaatag ccattgttgc agaaatcacg gagattactg
    27781 aagaggctgt ttatctgaaa gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt
    27841 cgattaccat agcgagtggc tatcctaaag gggaaaagct ggattggatc gttcagaaat
    27901 ccactgaatt aggagcgcaa gcttttcttg gtttcccggc atcagcctct gtcgttaaat
    27961 gggatgcaaa aaaacgtatg aaacgacaac aacggcttga aaagattgct caagaggcag
    28021 cggaacaatc ccatcggcag atcgtgccat cagtagagtt attggaaacg aaacaagatt
    28081 tatttgagcg gctagatgat tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc
    28141 aaggcgaaaa atcaaatgca gccaaaattc ttagtgaaac aaaaccagat agtcgaatct
    28201 tagctgtctt tggaccagaa ggtggattat cccgcgatga agtcgattat tttgtagaac
    28261 acaaagggat actttgtgga ttaggaccaa gaatccttcg tacagaaaca gcacctcttt
    28321 atttattaag tgctgctagc tatcaatggg aattaatgaa tccttctttc agctaacgtt
    28381 cttcattaat aaaagagtat cgacagaaag aattgaaaaa atagtcaaca atttgtataa
    28441 tagaaacaat tatttataca atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg
    28501 aacatttccg taaaaaaacg tataactgca cggattttgg gacaattatc aaacagatat
    28561 ttgtcccgat tcccgtttta ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa
    28621 gaaattttaa cgggacccgg tgtcataaag ctcgttagtc aatacatggg tcccgaacat
    28681 gtagcatttg ttgagaaagc atgcgaatac gcgactgctg cacatgacgg acagtttaga
    28741 aaatcaggcg aaccctacat tattcatcct atccaagtcg caggtatatt agcagattta
    28801 aaaatggatc cccatacagt ggctacaggc ttcttacatg atgttgttga agatacagaa
    28861 atcactttag aagatctgag agaagaattt ggcgatgacg ttgctatgtt agtggacggc
    28921 gtaaccaaat tagggaaaat aaaatataaa tcccacgaag agcagctagc agaaaaccac
    28981 cgaaagatgc tgctagcaat ggctcaagat ttacgagtta tcatggtcaa attagctgat
    29041 agactacata acatgcgtac gttgaagcac ctgcgagaag ataagcaaag aaggatcgct
    29101 caggaaactt tagaaatcta tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa
    29161 tgggaattag aagatacagc acttcgttat ctaaatccaa aacagtatta ccgtatcgtc
    29221 cacttgatgc agacgaagag agaagaacgt gaaaaatacg taagtggtac tgttgaagat
    29281 attcgaatag cgacagaaga gctggggatt tttgcagaaa tctatggacg gccaaaacac
    29341 atttattcaa tctatcgtaa aatgaaagat cagaaaaagc agttcaacga aatttatgac
    29401 ttgctagcta ttcgagtgat cgtagattca atcaaggatt gttatgctgt attaggagca
    29461 atccacacaa aatggaaacc aatgcccggc agatttaagg attatattgc catgccaaaa
    29521 gctaatatgt accaatcttt gcatactacc gtgatcggtc cggcaggaaa tccggtagaa
    29581 attcaaatcc gaacacagga aatgcatgaa atcgctgaat tcggggttgc tgcacactgg
    29641 gcctataaag aaggaaaaaa tgaaaaagta gaaccagatg gtatgacgaa acaattaagc
    29701 tggttccatg agatactcga acttcaagac gaaagctatg atgcttctga atttatggaa
    29761 ggcgtaaaag gagatatctt tagtgataaa gtctacgtct tcacaccaaa aggagacgtt
    29821 actgagttac caaaaggatc cggaccattg gactttgcat acagtatcca tacagatatc
    29881 ggtaacaaaa ccactggtgc aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg
    29941 aaaaacggag atatcattga gatcatgact tctccaaatt catttggccc aagtcgcgac
    30001 tggttgaaat tagttgctac tagcaaagca agaaataaga tcaaacgttt cttcaaagcc
    30061 caagatcgag aagaaaatgt gatcaaaggc cacgaatccg tggtcaaatg tattacagat
    30121 ctaggattta cgcctaaaga tattttgacg aagaacaaac tgcaagaagc actcgatcgt
    30181 tttaattatc aaacagaaga tgatctctat gcagctgtag ggtatggaga agttagcccc
    30241 ttgacgatgg ccaatcgtct gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa
    30301 aagcaagaag cagaagaaat catgaatcag ccgaaaaaag aacctgacaa aatgaaagta
    30361 cgtcatgaag gtggcgttgt cattcaaggg gtagaaaact tactaattcg tatcagtcgc
    30421 tgctgtaatc cgattcctgg tgatgatatc gttggttata tcactaaagg cagagggata
    30481 tccattcatc gtcgagattg tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt
    30541 ttgattgaag tcgaatggga agatacatcg aatacacgaa aagagtatga tgcagatttg
    30601 gaaatttacg gctataatcg ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg
    30661 ctaacgaaaa atctcaacag cgttgaagca cggacgaata aagataaaat ggcgacgatc
    30721 catttgacgg ttggtatcca gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa
    30781 gcagtacctg atgtctacag tgtacgccgg acgaatggat aggaggacag atatgagagc
    30841 agttgttcaa cgggtaagta aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat
    30901 tggctacggc ctacttgttt tattaggcat tcatgaaacc gatacgcaaa aagatgtgga
    30961 ctatctggta aaaaaaattg cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa
    31021 tattagtgta gaagatgtgg gaggacaaat tttaagtatc tctcaattca cactgtttgc
    31081 ggatacgaaa aaaggaaatc gaccaagctt tgtggaagcc gcacgaccag aaacagctat
    31141 tcctttatac gaatcattca atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg
    31201 acaattcgga gcagatatgt cggtttctct cgtcaatgat ggacctgtaa cgatcattat
    31261 tgattctaaa aacaaataaa gtaatctggt tttcactagt aaaaactatg aaacacgtcg
    31321 cttaagttag actgataaac tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct
    31381 tttgtcagca gtcggaaaaa aagttaagct taaattatac aagttatttt ttataggagg
    31441 ttgctgaaga tgaaagaagt accagtagta gatatcaaaa tcacagattt aaaaaaattg
    31501 tatcaaaaag gatacaacat gttggaggag ttacgccatg aagcggacgc tcccgtcgtc
    31561 aaagcaaaga ttttcaataa agaagctatc acgatctacg gatcatcagc agcaaaagta
    31621 ttttatgatc caaggaattt caaacgaaaa ggtgctatgc caaaattagt attgaagaca
    31681 ctttttggtc aaggtggagt acagactttg gatggagctg ctcatcacca tcggaaaaat
    31741 atttttatgg atctaatgac accggaaaga atggaagatt atcatcgtat cttagataag
    31801 aatctgacac aagcacttga agcacagcat ggtcaatttg aactatttga tttaagtaag
    31861 atggttttct ttacatctat ttgcgagtgg gcaggtatca atttatcagc gatttctaaa
    31921 gatgaggtcg aaaaattagc agaatatcaa atctctatga tcagcggaac gtttacttct
    31981 ccgattgatc acataaaagg tgtcgaaaat cgcaaaaaat cagaaaaatg ggcgcaaggc
    32041 ttgattgaag aagctcgaca aaatcctgtg gctggaaaag aaaatgtggc attatatgct
    32101 tttgccaatg ccacagattt agatggtcag cttttacctc tggaagtggc agctgttgaa
    32161 ttattgaaca tcatccgtcc aacggttgct ctaactgtgt gggcggcatt gatgggacat
    32221 gcacttttca gtagaccaga tttgtatcag caattaaaaa atgacttttc gacgttacaa
    32281 gatccgttta ttcaagaaat gagacgctat tatcctttct tcccaatgct acctgcgatt
    32341 tcgctgaaag aagtagaagt agatggttat cgtataccag aaggcagttg ggttatttta
    32401 gatttatatg gaacagacca tgatgaacga acagttgaag cgccagattc ctttatgatc
    32461 aaacggtatg tagggaaagc aaaagatatt tcctataaag aagaatatga gatgattgca
    32521 caaggcgggg gaaattttcg acaaatgcat cgctgtgcag gagaatggat cactcttcat
    32581 agtctacgtg tgttttccga tcagctagta aataagtttg agttctctgt acctgaacaa
    32641 gattggacga ttccattcaa tcagtttccg acttatccga atagccgagc gctactttac
    32701 aagaattaga ttagcataaa cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc
    32761 ttttttcata ttactataaa ttgataaggt caattgatca aaatccacga aatgcttcat
    32821 caatggtttg ataatcttca ttggataaat gcaaatctaa agcttggaca ttgtcagata
    32881 cttgctctgg acgcttagct ccaggtataa ctacagacac tcgtggattc ttcatgtacc
    32941 aagctaaaac aagttgcgca ggagtggctt ggtatctttt agcaatgggg cgaagtacat
    33001 cgacagcggt taaaatttct ctgaaacgtt ctccttggaa atctggattt ctttttcgag
    33061 gatctccttc accaaattgt ttttcctcac caagttcata ttttcctgtc agcaaaccgg
    33121 aagcaagagg aaaataggga acaaaactga ttttgttttt ttcaagataa gggaataatt
    33181 ctttttcagc ctgccgatgg atcaaactgt atttatcttc tactacatcg acatatccat
    33241 ctgcattcgc ttctttcaat tgttctaacg tgaagtttga cacaccgact gctcggattt
    33301 ttcctgcttc ttttaattcg tgcaatgtag caactgattc attttttggt gtgctttcat
    33361 ccggaaagtg gatataaaaa atgtcgatgt aatcagtttg cagacgtttt agtgcatctt
    33421 ccaccgattg tttgagaaac tcaggagaat tatcaaatgt tcgcccttta tttggtacat
    33481 gagcagcttt tgtagcgatg acgacacgtg agcgatcata ttctttttcc ttcagtacct
    33541 cgccaatcag ttcttcggaa cggccgtttc cgtaagcata agcggtatcg atcaaattga
    33601 tcccgctatt caaagctgta cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa
    33661 aaagattatg tcctccaacg gcatttgctc ctaatccaag ctgttctgcg tagacttgtg
    33721 aatgtccgat tcttatttct gccatagctg attcctcctt tttgtctagt ctaaaacaaa
    33781 ataccaaaaa gctgaactaa aacgtttttg tgaggggaaa tagtcagata tctcttgact
    33841 ttgagactgg tttttagtag gttataagta atatcgaatg tcaatgaaag agcgagtaag
    33901 tttttgaaac tttacaagag aggatcatcg aggctgaaaa tgatctaaag tttcattagc
    33961 ctaaagacac tctggagatg gacgagtgaa aagccgtccc gcgtacgagc gttattcgta
    34021 agaggaaaga gaaatctttc aaaattaggt ggtaccgtgt atgaatttca ttcacccttg
    34081 ttcatgatga acaagggtga tttttttatt tacatagcta agaaagggag agaaaactat
    34141 gagtttccaa agaccaaaag gaacaaatga tattttgcca ggcgaatcag aaaaatggca
    34201 gtttgtcgaa gaaacagcac gtttattatt cagagattat cagtataatg aaatgcgtac
    34261 accgattttt gaacactatg aagtgatatc ccgaagtgta ggggatacaa cggatatcgt
    34321 ttcaaaagag atgtatgatt tttatgacaa aggggaacgc catgtgacgc ttcgaccaga
    34381 aggtacagca ccaatcgttc gttcttttgt tgagcataaa ttgttcggcc cagagtatgc
    34441 gaaaccgttc aaaacttttt atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg
    34501 acggttacgt caattccatc aaatcggagt agaagcattt ggatcagaaa accctgcaac
    34561 tgatgtggag agcatggtga tggcacttga tttcttcaaa cagttgggga tcacgcaaat
    34621 ccggctagtc atcaattcat taggtgataa agaaacgcgt caagcctatc gtcaagcatt
    34681 gatcgactat cttttacctt ttgaatcaga gctgagtgaa gactctaaac gccgccttca
    34741 tgaaaatcca ttacgcgtat tggacagtaa agacaaacga gatcaaaaat ttgtggcaga
    34801 agctccatct atcttagatt atctaagcga gcctgctcaa aaacattttg ataccgttgt
    34861 ttccatgctg gatgcattgg ctgttcctta tgaaatcgac agtaatatgg ttcgcggact
    34921 agattattac acacatacga tttttgaaat catgagtgac gcaccaaaaa tgggggcaca
    34981 agcaacgatt tgcgcaggcg gacgttatga caatctagtg gaagaactag gtggtccttc
    35041 aacacctggc ttcggatttg caatgggtat cgagcgatta ctcttgacta tggaggcaga
    35101 ggaagtagtt atccctgcat ttaatgagtt ggatgcttac gtggtcgcac tcggagacga
    35161 aacaaatatc gaagcattga aagtggtcca agcgattcga aactttggtt tttcagctga
    35221 ccgcgacttc atgaaccgta aagcaaaagc gcaatttaag acagcagata aagcgaatgc
    35281 caaattagta ttggtgatcg gtgaagatga gcttgctagt caaacgatta atgtcaaagc
    35341 aatggcaagc cgaaaagaaa aaagttttcc attagaagag atatatgaac attttgatga
    35401 agtctatgat gaaatgactt tattcgagta ggaggaaaaa aataaatggc acaaagaacg
    35461 atctattgcg gattagtttc cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg
    35521 gtacaaaaaa gacgagacct tgggggagta atctttatcg acctacgtga tcgtgaagga
    35581 atcgttcaaa tcgtctttaa tcctgaaatc aacaaagaag catgggaaat cgcggataaa
    35641 tgtcgcagtg aatacgtgat cgaagtgact ggggaagtta gaagacgcgg agaaaatgca
    35701 gtaaatccta aaatggccac tggtgaattc gaagtgattg caagtgaaat cgtgattttg
    35761 aatcaagcaa aaaccccgcc atttcaaatt gaagatgatc aagcaatcag tgacgaaata
    35821 cgtttgaaat atcgttattt ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta
    35881 agaaatgcga caacaaaagc gattcgtcat tatttagatg ataatgactt tctcgatatc
    35941 gaaacacctt acctagggaa atctacacca gaaggcgcaa gagactacct agttccttca
    36001 cgtgtccatg ctggacattt ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg
    36061 ctgatgaatg ctggattcga ccgttattat caaatcgttc gttgtttccg cgatgaagat
    36121 ttacgtggag accgtcagcc tgaatttacg caagtagata ttgaaacaac gttcttaaca
    36181 gcagaagaaa tccaaactta cacagaggga ttgattgcga aggtaatgaa agaagttcgc
    36241 ggtatcgaag tcacattgcc atttcctcgt atgacctatg atgaagcaat ggctcgctac
    36301 ggcagcgaca aaccggatac acgttttgat atggaattga tcgatctaag cgatacagtc
    36361 aaagaagtag aattcaaagt cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca
    36421 ttgaatgcta aaggcgctgc agatcgctac tcacgaaaag acatggatca gttaggtcaa
    36481 tatgtaggac aattcggagc taaggggctt gcatggctga aagtagaaga agacggattg
    36541 aaaggtccaa ttgctaaatt catgggagaa gcaacagaag caatcattaa agcaacagat
    36601 gcaaaaccag gtgacttgct gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca
    36661 ttaggtgcta tccggacacg tttaggaaaa gaattaggac taattgatga atcgaaattc
    36721 aatttcttat gggtaactga ttggccacaa tttgaatttt cagaagaaga aggtcgatat
    36781 gtctctgctc accatccatt cacgatgcca aaagaagaag atatcccgtt attggctgaa
    36841 gaccctgcaa aagtctatgc agaagcttat gatatcgtct tgaacggtta tgaactaggc
    36901 ggaggttcta tccgtattca tactcgtgag cttcaagaaa aagtactaga aactctaggc
    36961 tttacaaaag aacaaatgga agaacaattt ggtttcttgt taacagcttt agattacgga
    37021 ttccctccac atggcggtat tgcattagga ttggaccgtt ttgcaatgtt attagccgga
    37081 gaagataata ttcgtgaagt cattgccttt cctaaaaacg gaaaagcagc agatgtgatg
    37141 agtgatgcac caagtatggt ttcgccttta caattgttcg aattgaatat tgatgtaacg
    37201 aatatagaag aatagccgat aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac
    37261 aaccttgatt taattgaaaa acactttata atgaagccat aacatataaa gcggggcttt
    37321 tccatgaaaa aaacattcga aaattgggct tatcatgatt acacgaccaa gctttcggta
    37381 tctatcgttt atgcgttgat cgcatcagtc gcattgaatt tcttttatca gccaggacat
    37441 atttattcaa gtggtattac tggtctagct cagattttga cgactttgtc aaaatcactg
    37501 ataggattcg aagtaccggt atcaacaact ttgtatttac tgaatatacc tttgtttgta
    37561 ttggcgtggt taaaaatcgg gaagaagttt acaattttca cttttatcac agtcactttg
    37621 acctcccttt gtatccatat tttaccggaa acagtcctat ctaccgatcc aatcatctgt
    37681 gcgttgtttg ggggggctgt gatgggacta ggtattgggc tagcgttgag aaacggccta
    37741 tcatcgggcg gtctagatat cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt
    37801 ggttctattt cgatctactt caatgcattg atcgtttttg ttgccggaat tctatttggc
    37861 tggcaatata tgttttacag tacgttgtct attttcgtca gtggaaaagt aactgatgtc
    37921 gtttatacta aacaaaagaa gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta
    37981 gtaaatgaaa tccaaaactg tatgcgtaga gggatcacga tcatcaatga agcagaaggt
    38041 gcttataagc atgataagca gacagtattg atgacgatcg ttacacgata cgaactgcct
    38101 ctacttagag aagcaatgaa agcttctgat ccacaagctt ttgtaagtat tgcagaaaac
    38161 gtccagatac ttggtcgttt ctatgaggat ggcatttaga tcgagtttga caaaaattca
    38221 tgaaatactg ctgtagacag acgttcgttt gtctgcagtt tttttctacc atagtagaat
    38281 cagcaaagaa aaactgcttt tttatttgaa attttcttaa agttgaaaaa aagcatgttt
    38341 atttttttaa atttatggga tgatagaaaa aaatagtaga gaaaggacac gattctactt
    38401 gctattttca cttaataccc gtatattacg aatgaaaaaa gtttatgaaa agttggaata
    38461 ttgaatggaa aaacaaaaaa ggttaaaaaa cagccgctac atcactgggt ttgatgggct
    38521 ccgttcatta gctgtggtgg gtgttatctt ctatcacctt ctgccaacta gtatgaaagg
    38581 cggctatttg ggcgttccga tttttttcgt cgtatctggt tatttgatca cggatctgct
    38641 gcggcaggaa tgggaacaaa atggaaaaat caatatttgg caattttatg tcagaagaat
    38701 gaagagactc tatccggcac ttgtttttat gcttgtgaca gcttcggctt atatcacttt
    38761 gtttcagcga gggctgttaa acaatcttcg aggagttgtg gtcagcagct tactgtatgt
    38821 caacaactgg tggcaaatca ataacggttt atcttatttc gatcgatttg ctaatgagtc
    38881 accgtttacg catatctggt ctttagcagt agaaggtcaa aactatttgg tttggccgat
    38941 cttgtttgta ttgttgatgg tatttgttcg taaaagaaaa tggatctttt atactgtgtt
    39001 aggtacatcc ttcctctccg ccatcttgat gatgatcttg tatacaccag gaggcgatcc
    39061 aacccgtgtt tattacggta ccgacactcg tctgttttct atatggttgg gaagtgcgct
    39121 tgcgtttgtt tggccaagta cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat
    39181 tctgaatatc gctggaggaa tctcgctttt ggcactgatt ctttgcttct tctttttaga
    39241 tgatcattac aatttcatct attacggagg aatgctccta gtgagtctgt tatgcgtagt
    39301 gctagttgca gtgacagcac accccggagc aagtttgaat cgatggctga caaatccagt
    39361 atttacttgg attggcaaac gaagctatgg catttatctg tatcagtttc cagttatgat
    39421 tttttatgaa gcaaaggtga ctgatattgc cgatcatgtt ttgttgcata ctttagtgga
    39481 aatcacattg attctaatca tcagtgaact ttcttatcga tatgtggagc gtccgctagc
    39541 acgttttgat tatggtcgta cattccaagt gatgaaagga tggttcagca aaccgatcat
    39601 cagtaagagc aaaccatggg ttgttccagg cacattactt gtagcaattg cactcgttgg
    39661 tttcgttacg gcaccgaaaa acgctgtaac agccgatcaa aaacaactgc aggcaaaaat
    39721 cgctgaaagt aaaaaattag cagaagaaac cagaaacaat tcagaaaaca ccactagttc
    39781 ggttgaccaa gcgatccttg ataagtacga gttgtccaag gcacaaggga ataaggcaca
    39841 aaaattagaa ctaacggcat ttggtgattc agttatgttg gatgctgcaa cggatctaca
    39901 agaactttat ccaaaagtag tggtagatgg agatgtcgga cgacaattat atgccagtgt
    39961 accgtacatt gaaaaattaa aggaacaaga tttgttgaaa gacactgttc tgatcggact
    40021 aggaacgaat ggctcattct cggaagctca gtttgatagt gtgatggatg caatcggtga
    40081 tcgaaaagta tactgggtca atgtccgagt accgacgaag cgctggcaaa atgacgtgaa
    40141 tgcgatgctt gaacagatga cgaaaaaata cgacaatatg acgctgattg attggtacag
    40201 ctacagtaat gaccatagtg actggtttta tgatgatcaa gtccatccaa atcctgaagg
    40261 gatgacacat tacattcact tagtcagcga agccttgcta ggaggaagtt caagtacgaa
    40321 cagcagtgag acaaacgtga cagaatgata gctacaaaaa acttcaggca aaaacatttg
    40381 tttttgcctg aagttttttt atatttaccg tgtttttaac attcatatag attcaggaag
    40441 caaaacggtt gagtgtgtct gtaaataacg ttctttttgt tttcgggtga gttttttgaa
    40501 tgcagcttct gcttttgttg cttcgcttct tgaagaaaat acttctgcat ggatcatctt
    40561 aacaggtctt cttttttgaa gacgagtata tttagcacca gtaccattgt tatgttcctc
    40621 taatcgtcta gaaagatcgg ttgtatatcc accataaaat gtctgatctt tacagaataa
    40681 tacgtaaaaa taatgttcat ttgccataga gcatctcacg aatctctgca gtgtattctc
    40741 catccatatt gtaagtgatc aatggtggag cgactttgaa tccgtctaat ttgccttctt
    40801 tgattccttc aactaacaaa atatttgcct cttttccagg ttttgggtaa acaaattgga
    40861 tccttttagg tgcaattcga tagtcttcca tgacctgaat gatttctaag aaccgatcag
    40921 gacgatggac catagtaaag cgtccatttg tttttagtag cttacttgaa atatcgatca
    40981 cttcttctaa cgtcgtatga atctcatgac gggcgattgc caggtgttta ttagggttcg
    41041 ttttatttgt tggcagccct ttgaaataag gtggattaca gacgactaag tcacaagaat
    41101 tatgtcgaac gacagataag ctgtctttca gatcgatggt gtggacagtc atttgttcat
    41161 ttaaatggtt aagcgcgata ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga
    41221 tagcgtcgat agccgcctgg gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg
    41281 cacaaaggtc aacaatcttc ccttttttag gtacacggga aaagtgagca agcagcacag
    41341 catctaaaga aaaagaaaac actttggaac tttggataat cttgatatct tctgcgtata
    41401 attggtcgat tcgttcgtct tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc
    41461 gtctgtatta tactattttt attaaaaaga accaaggtgg cttgtcaaac ctctattttt
    41521 tatgcatact atgaccataa gaagggaaag gaagagacta tgttttatcg atttatgcgc
    41581 ggggtagttc gagtactatt agccttagtt aatggaaatg cccgctatga gaataaagaa
    41641 gttttaccgc aagatgagaa ttacatacta gttgctcccc atcgtacttg gtgggaccca
    41701 ttatatctgg cggtagctgc agcacccaag gaattcagct ttatggcaaa agaagaactg
    41761 ttcaaaaatc cagttctgcg atttatatta actcgatcca atgcttttcc agttaaaaga
    41821 gaaaatccag gaccaagcgt cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg
    41881 agtttgatca tgtttcccag tgggacaaga catgctacag aacttaaagg cggaatggca
    41941 ttgatttcca gaatgtctaa agcgaaaatc gtgccagctg tatatcaagg tccgttgaca
    42001 cttggggatc tgttcaagcg tcagagagtg acagtgcgtt tcggcgaacc aatcgactta
    42061 tcagatatca agaaaacgga taaagaaggt atggcggaga tcgaacgacg aacacaagca
    42121 gcttttgaca aattggacaa agaagtgaac cctgatttca aatatgaagt caaaaaaaaa
    42181 taaaatgata agtttatgcg tgggcggtct tttgaatcta ctgtcctgtt cagttcagac
    42241 tttttcaatg aaaacgaggg agagacgtca agtctgccct ctttttgcgt tccaatatgt
    42301 aaaatagaag acaggtgtca tgtaatggat caaagtgtta taataattag aaggacattt
    42361 tttgaggtga ttgaatgcgt tttttacata cagccgattg gcatatcgga aaaaaactac
    42421 aaggttatga cttgattgaa gaccagcgtc atgtactatc ttctatacta gatatcgcga
    42481 aagaagaaaa agtagaggca atcgtgattg ccggagactt atatgatagg agtgtcccag
    42541 ctgttgatgc cgtagaatta tacaatgaat tgatggtcga ttggaattta aaggaaaaga
    42601 tcccgatttt tgctatatca ggaaatcacg atagcagtac gagactttca gcgggtacgc
    42661 catggttcag ttattcgaac tattttttgc gaacacagct agcagaagca tttgaaccag
    42721 tcgagtttaa aaacgtacaa tttttctttc ttccttactt tgaaccaatt gcagcgagat
    42781 tatattttga agatgacaat atccgaacga tcaagcaagc ggtagccaag gtgatagata
    42841 aaatgcagga gctatttgat tcagaaaaaa aacaagtatt gattagccat ttttttgtaa
    42901 tgggaagtgt aaaatctgat tcggaaacga aaatcgaagt aggcgggttg gattcgatcc
    42961 ctggtgaact acttacaccg tttgattatg tagcactagg acatttgcat aatcaagctg
    43021 ctttgagaca ggataatgct cgctatagcg gttccccatt gaaattctct ttatctgaga
    43081 tcaaccaaaa aaaaggcgta tggatcgttg acctgacaac aggcctggag ttgaatttta
    43141 gggagatcga acccctgcat gatattgaag aaataacagc gagtttcaaa gaattgctgg
    43201 atcctgcctt ttatcagtcc gtcaatcggg agaattacat ctatatctat ctcactgata
    43261 gaacgatcat cccgaatatg atgaaccagc ttcggcaaat atatcctcgt attttgggga
    43321 ttgaacgggc aaacggtcga gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga
    43381 aagatcctca aaaattagca gaagcatttt ttagtgaaat gacggaagag cagctaactg
    43441 caaaccaaga aaaatggctc ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa
    43501 gaatgaaacc gaaaaaatta caacttaaaa attttggtcc ttttatcaat gagaccgtag
    43561 atttttcacg tttgacagaa gcaccgcttt ttttgatcag cgggaagaca ggagctggca
    43621 aaacgacgat ttttgatggg atcaccttcg cactttttgg tgaaacttcc ggaagacttc
    43681 gctctggaaa agagatgcgt tctttgtttg ctacaccaga cgaagaaaca agcgtgactt
    43741 tttcttttga acatcagcag atgagctatg aaatcgaacg aaaaccagaa caagttttag
    43801 caaaacgaaa aggaaacgga acaaaaaaac aggcagccaa agtaacgttg accatttttg
    43861 atgggaaagg aaaagaaata aaacaaatca caaaacgtac agaagcagat cagttgatca
    43921 gagaactgat gaatctcgat gcgaaacagt tttctcaaat cgttttacta ccccaaggag
    43981 aattccgtaa ttttttggtt tcatcaagca gtgataaaga gactgtttta cgtcatttat
    44041 tcggtactca atttttccaa cagttcaatg agcgattaaa agaaaaagca aaacaacagc
    44101 aacaaaaatt ggatcattta gagcaagagc tgcatcttct gcaaaaaagg tttgttttag
    44161 tcaaagaaga ggaaccagca gcagggtttg aagccgtttt agcccaatgg acaaaccatc
    44221 aggcaatact cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa cttcaggaaa
    44281 aacaaaagca actagaaaat tcctactatc gttttgaaag cttgaaaaaa agttatgaag
    44341 aaaaaacgat attaataaac aagcaaaagg aattagctgc tcaaaaagac gaaatagagg
    44401 aaaagaaacg atggattggg tattatgaat ttacgcaacg attagtcgaa ccaatccagc
    44461 gcctacaaga aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa
    44521 agaaactagc gatgactacg cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg
    44581 tacgccgaca aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg
    44641 tgccaattgt cgaatcctta cgtagtaaaa aacaagacgc agaacaatta acaatttcat
    44701 tggataaaga agaattgaat gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa
    44761 gaactcgaat tcttcaagaa aatttactgg atcaggaaaa actgcaagaa gatcgtcttg
    44821 cttttggaca gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa
    44881 aagacactgg gtcgctcgca cagataagag aagaactcaa aaaagcaact gaagaaataa
    44941 caaaaaatag tcagctattg aaagaacaga cagaacaagc agaacagctg aaaagccaat
    45001 gggcaaagca gcaaattgct cgtcttcagc tttttttgcg tccaggggag ccatgtcttg
    45061 tttgtggatc aacggaacac cccatacaac aattaaaaca cgaacagcca aataaggaag
    45121 atatcttaaa agtagaacaa caactggcag aagctgagtc tgctgtagaa agaactcagc
    45181 aaatcattgc caaaagtgaa gcacatgaaa ataatcttca acaacaatta tcagatttag
    45241 aacaaaaaat catggcaacg aatatacaat tggctgaaca agaagaacac ttgcaacagg
    45301 ctttgaaaaa ggcatttcct gagataacag aacaaaaaac aaaaacaact cttgtagtaa
    45361 tcgaacaatc tttagataga cgaaaaagca agatggccaa tgcaagaata gagctggaag
    45421 aaataagaca acaagcagaa aagctcgaac aacaattagc tgtctacaaa cagcagcaga
    45481 cagaaaaaaa taatcagctc cagcaactgc aaggagaaat caaaagttta gaacaacaat
    45541 tgcaagatac ggatagtaac tgtttagaag aggagatacg acgacttgaa aaacagttag
    45601 aagaagactc gaatcaacta gctgttgagc agaaagcagg agtgcaattg ttgcaagatc
    45661 ttcatacgct tcggacacag cttgatcttc aacatgaaca aatagaaaaa atagatgaaa
    45721 aaatagaaaa ggaacaagaa agaatagcta aaaagttaga aagccagtcg tatttttcaa
    45781 caattgaaga aatctgtgca tttgcacaac agcaagcaca atataaagaa aaccaacgta
    45841 cagttacttc ttatcatgaa gagcaattag tgacagctga ccggctgcgt caattagaaa
    45901 atatccagct aactgagaca ttcccagata cagaagagat cagccaacag ttagaagaaa
    45961 taagacgtat gatcgaaata aagcaagaaa agatatacgg tttacaagaa caaaaaatta
    46021 gtaatcaaaa aatctacgat gaatgtcttt cgatttatca cacaagccag acgaaattag
    46081 atgagcttac gcaattacag cagctttcgc aaacattcaa cggagaaaat cctaagaaga
    46141 ctagtttgga acggtatgtg cttcaggttt atcttcaaga agtgttacaa gtagcaaatg
    46201 atcatctcca acgtttaacg aaaaatcgtt atcaatttga gctagcggat accattggca
    46261 gttatcgagg gaaaaccgga ttagaaatca atatctatga tgatcaggca ggtatgtctc
    46321 gaagtgcgca taccctgtct ggcggtgaga gttttattgc agcactttct ttagcattag
    46381 ctcttgccga tgtgatccag acacaagctg gtggtgtagc aatcgatgca ttgtttattg
    46441 atgaaggatt tgggtcatta gatgaagaag ctttggaaat ggcaatggaa gctttagaaa
    46501 cgatcgaaag tgaaggaaga atgatcggca tcattagtca tgtaagagaa ttgaaagaac
    46561 gagtattaca acaaataaga attgatacag aaggtagcgg acaaagcaaa atacgctatc
    46621 aacttggata aaaagggaga ttggtaacta tgaaatggag tattccagaa aaagtcattg
    46681 cacgtggacg tcaatacttg caagaaggcc gagtgttatc ggttgtccct gaccaagaag
    46741 aaaacgtgtg gcatgcagaa gtcttgggca gtgaattgta tatggttgat ttggatggta
    46801 cagcaaaaga gatagattac tgccaatgtc cctattggga ggaacataaa tattgtaagc
    46861 atacagttgc tgtggaactt tatttacggt cgaaaaatct atctcgtatc atgaaaaaag
    46921 accagccaat gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt
    46981 tttctcgtct aaaaacacaa aacaatcctc aagcggtgca acctttgatt attgaatacc
    47041 aagtagaaac gattgagact aatcagtatc acccagagct ttctattttg gcactttact
    47101 tgcgtatcgg ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt
    47161 ttttacaagc ttacacagaa aaagaaactt acactgttaa taaacagtac cagtttcgtc
    47221 tagataaacg ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt gcaggaagtg
    47281 ctcagacgca tcaattatta ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa
    47341 gatatctgct tctcccagta gaacaagccc gcttcctgtt agagcagatg aatcagacta
    47401 cacgtttgca tctgcagata aatgaacaaa acgatcaagg aatacaattt tccgatcaaa
    47461 acaaaccttt atcattcaaa gtggaaaaat ttggagaaga ttatttgctg caagcattga
    47521 atgattttga ttttttcttt atgcattatc agtggggagt tatccaaggg acattttatt
    47581 ctctgacgaa atatcaacaa acggtttatc aaacttggaa acaattgatt cgccgattgg
    47641 agaaaccaga agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa gagattatcc
    47701 ctctgctgtc agagatcggc gacgtagcta ttgcccctgc agtagaagag gaagtagcag
    47761 atatccccgt cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag
    47821 tcgatttcgt ctatggcgaa gtcatctatt cgactgaccc gaagcatgag gtctcgacag
    47881 aacaggcttt tcaggtgtta cgtgacacag tagaagagaa acgagtactt gatctattca
    47941 gaatgtatcg ctaccagaaa aatgaaactg gttatgaacg tccattgcca agcggggaag
    48001 aactatatgc ctttttccgc acagaactat ctgtcttccg tcagtttggt gaagtcagac
    48061 ttggaagaaa attgcgagag ttgtatttag atgcgcataa acatcaaccg aaaatcgaag
    48121 taaccgagac cggttcatgg ctcgatattc gattcgatgt gacagggata gaggagcaag
    48181 aaatcgatca tgtcctgcaa agcttgctga gaaatgatgc tttttataca ttagaaaatg
    48241 gtcaggttct ttcttttgat tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat
    48301 tacgggaatc gatacgtaca gaagaaggaa cgatccatgt acctaaaaat caaggattga
    48361 tcattcaaaa tcaattggaa aaaagcaatg ctacttttag tgaatcgttc caaacgatgg
    48421 tacaagatct cattcatcca gaaagatacc aggcacagct tcccaaagga ttgaatgcaa
    48481 cgatgagaga ttatcaaaag caaggttttc gatggttgaa gatgttaggt cattatcagt
    48541 ttggcggtat tttagccgat gaaatgggac ttgggaaaac actacagaca attgcctttt
    48601 tactttcgga aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg cctgcgagct
    48661 taatttacaa ctggcaggca gaggtgagaa aattcgcccc ttctttaagt atccaagtaa
    48721 tcaatgggaa taaaaaagaa cgtgaagaat tactggctaa agacacagat atccgagtga
    48781 cttcttatgc cagcttaaga caggatctag ctaattatca gtcgcaaaaa attgattatt
    48841 tgattcttga tgaggcacaa atggtcaaaa atagcagtac gaaaactgca caagcattga
    48901 gagaattagc tgtacctcag cggtttgcac tgagtgggac accaatcgag aataatttag
    48961 aagaattgtg gtcattgttt gcaacgatta tgccaggatt tttcccgact aaaacaaaat
    49021 tcagggaact tgccgcagaa gaaattgcgc aaatgattcg accgtttatt ttacggcgag
    49081 ataaacaaac tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta tattctgcac
    49141 ttacagaaga acaaaaaact gtttatttag cttacttgcg tcaaatgcgt gaagaaatca
    49201 catcaatgga ttctgaagca ttcaagaaaa accggatcgg gatacttgct ggtctgacaa
    49261 gactgcgaca aatttgctgc gatcctcgtc tgtttattga agattaccaa ggcggttccg
    49321 gcaagctcga acaagtaaaa gatctactgg tagctgccaa agagaacaaa cgaagagttt
    49381 tgctgttttc tcaatttaca gggatgctga ctatcttgca agaagagtta gccgagctag
    49441 ggatctcgac attttattta agaggcagta cgaaaccaca agatcggtta agtatggtag
    49501 acgcatttaa tgctggcgaa aaagatgttt ttctgatttc cttaaaggct gggggaactg
    49561 gattgaatct cacgggagca gatacggtca tcttgtatga cctttggtgg aatcctgcca
    49621 ttgaagaaca agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt
    49681 ggcgtatgat ctctgaagga acgattgagg aacgcatgga ttcactccag caagaaaaac
    49741 gcgaactgtt ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa ctgactgaag
    49801 aagacatccg catgatttta agtgtaggag aaatcgagga gtaagagact cgaaaaagcg
    49861 ttcagtaatg ggtaagaaag cctttctaaa gaagcgattg tttatttgat ttaaaaaata
    49921 gcaaggaggc tgggattcca ttttatccca gcttccttgc tatcattttt ttatttctat
    49981 ttttaaaggt tatacaaaga ttgtcttcat tgtctctaac agctgatcaa aagaagaaat
    50041 ctcgatatcg caaattggag tctgttcaat tttgcgttct cgatggttga accaaagcgc
    50101 ttgccagtta gcactttttg cacctagaac atcattgtcg taattatcgc caacgtataa
    50161 ggtctcttct ggaagcatat gaaattcttt ctcagctagc tgaaaaatct ctttttctgg
    50221 tttttggaaa cctgttgctt gagaaatgag catattgcgg gaaggaatcc aattgttcaa
    50281 ttgaagttgc ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga taattcctaa
    50341 tgggacattc ttttccttca agaaatcgag ggtttttttg acctcctcat gcaagcagat
    50401 attatcaagt tcttcctcgt aaattttttg aaataagagg ccatcttctt ccgtaatatg
    50461 agggtagtct aaatctttta gtgattggct gatccgatgt gctcgcatat attctagtgt
    50521 ccaatctcct gccattactt ttgggaagtt ttcatcacta tgatggcgga accggatata
    50581 tagtggatgc atatctgcat ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa
    50641 tggctgctgc tgatcataca tcgtatcatc aacatcaaac acaactgatt taatcaaaat
    50701 tttacacgcc tttcggtttt cattgatttt ttcacaaatc cttgactatt gtaacagaaa
    50761 aaattgtcta aaaaagctga taaataaagg tttttcttac tatatttcta tttccttgac
    50821 agaataaggt ttttatattt cagttttttt cactaaaaat cgtgaaacac gtcaataagt
    50881 ttgatgaaca aattaatcat tatggtagaa gtcgattgat ttattttaat gcaatctctg
    50941 tggaattttc gaaagattta tttttttaat acagtctcaa tataactatt caaacataaa
    51001 ataacgttat ttgatttatt tgtttatagt aaattttatt tagtaat
//
LOCUS       DACENJ010000002        38963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.002, whole genome shotgun sequence.
ACCESSION   DACENJ010000002 DACENJ010000000
VERSION     DACENJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.002"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..437)
                     /locus_tag="IW748_000047"
     CDS             complement(<1..437)
                     /locus_tag="IW748_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1102986.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVT"
     gene            608..976
                     /locus_tag="IW748_000048"
     CDS             608..976
                     /locus_tag="IW748_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1102987.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1093..1578
                     /gene="mraZ"
                     /locus_tag="IW748_000049"
     CDS             1093..1578
                     /gene="mraZ"
                     /locus_tag="IW748_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1102988.1"
                     /translation="MGDCGRLILVSGGMGGLSMLMGEFQHNIDAKGRLIVPSKLREEL
                     GEKFVLTRGLDGCLFGYPMSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQ
                     GRINIPSTLRNYAALTKGCVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFG
                     L"
     gene            1599..2558
                     /gene="rsmH"
                     /locus_tag="IW748_000050"
     CDS             1599..2558
                     /gene="rsmH"
                     /locus_tag="IW748_000050"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1102989.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            2563..2964
                     /gene="ftsL"
                     /locus_tag="IW748_000051"
     CDS             2563..2964
                     /gene="ftsL"
                     /locus_tag="IW748_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1102990.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2965..5157
                     /locus_tag="IW748_000052"
     CDS             2965..5157
                     /locus_tag="IW748_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1102991.1"
                     /translation="MSLKNKIRRFMEKKNLNPINNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5183..6145
                     /locus_tag="IW748_000053"
     CDS             5183..6145
                     /locus_tag="IW748_000053"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1102992.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6159..7520
                     /locus_tag="IW748_000054"
     CDS             6159..7520
                     /locus_tag="IW748_000054"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1102993.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            7546..8634
                     /gene="murG"
                     /locus_tag="IW748_000055"
     CDS             7546..8634
                     /gene="murG"
                     /locus_tag="IW748_000055"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1102994.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8649..9869
                     /locus_tag="IW748_000056"
     CDS             8649..9869
                     /locus_tag="IW748_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1102995.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10025..11350
                     /gene="ftsA"
                     /locus_tag="IW748_000057"
     CDS             10025..11350
                     /gene="ftsA"
                     /locus_tag="IW748_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1102996.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            11373..12614
                     /gene="ftsZ"
                     /locus_tag="IW748_000058"
     CDS             11373..12614
                     /gene="ftsZ"
                     /locus_tag="IW748_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1102997.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEIGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12631..13308
                     /locus_tag="IW748_000059"
     CDS             12631..13308
                     /locus_tag="IW748_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1102998.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            13322..13921
                     /locus_tag="IW748_000060"
     CDS             13322..13921
                     /locus_tag="IW748_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1102999.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13936..14223
                     /locus_tag="IW748_000061"
     CDS             13936..14223
                     /locus_tag="IW748_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1103000.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14272..15054
                     /locus_tag="IW748_000062"
     CDS             14272..15054
                     /locus_tag="IW748_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1103001.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15075..15782
                     /locus_tag="IW748_000063"
     CDS             15075..15782
                     /locus_tag="IW748_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1103002.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16044..18842
                     /gene="ileS"
                     /locus_tag="IW748_000064"
     CDS             16044..18842
                     /gene="ileS"
                     /locus_tag="IW748_000064"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1103003.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18891..20417)
                     /locus_tag="IW748_000065"
     CDS             complement(18891..20417)
                     /locus_tag="IW748_000065"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1103004.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(20432..21079)
                     /locus_tag="IW748_000066"
     CDS             complement(20432..21079)
                     /locus_tag="IW748_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1103005.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(21263..21592)
                     /locus_tag="IW748_000067"
     CDS             complement(21263..21592)
                     /locus_tag="IW748_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1103006.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            21769..22497
                     /gene="sfsA"
                     /locus_tag="IW748_000068"
     CDS             21769..22497
                     /gene="sfsA"
                     /locus_tag="IW748_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1103007.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            22513..23526
                     /locus_tag="IW748_000069"
     CDS             22513..23526
                     /locus_tag="IW748_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1103008.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            23526..24803
                     /locus_tag="IW748_000070"
     CDS             23526..24803
                     /locus_tag="IW748_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1103009.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            24866..27568
                     /locus_tag="IW748_000071"
     CDS             24866..27568
                     /locus_tag="IW748_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1103010.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            27720..28037
                     /locus_tag="IW748_000072"
     CDS             27720..28037
                     /locus_tag="IW748_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1103011.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28067..28387
                     /locus_tag="IW748_000073"
     CDS             28067..28387
                     /locus_tag="IW748_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1103012.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            28495..29955
                     /locus_tag="IW748_000074"
     CDS             28495..29955
                     /locus_tag="IW748_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1103013.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30023..30244
                     /locus_tag="IW748_000075"
     CDS             30023..30244
                     /locus_tag="IW748_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103014.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            30275..30457
                     /locus_tag="IW748_000076"
     CDS             30275..30457
                     /locus_tag="IW748_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1103015.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            30457..30870
                     /locus_tag="IW748_000077"
     CDS             30457..30870
                     /locus_tag="IW748_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1103016.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            30993..32174
                     /locus_tag="IW748_000078"
     CDS             30993..32174
                     /locus_tag="IW748_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1103017.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            32563..32636
                     /locus_tag="IW748_000079"
     tRNA            32563..32636
                     /locus_tag="IW748_000079"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32597..32599,aa:Arg,seq:cct)
     gene            complement(32705..33844)
                     /locus_tag="IW748_000080"
     CDS             complement(32705..33844)
                     /locus_tag="IW748_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1103018.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(34143..34778)
                     /locus_tag="IW748_000081"
     CDS             complement(34143..34778)
                     /locus_tag="IW748_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103019.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(34891..35526)
                     /locus_tag="IW748_000082"
     CDS             complement(34891..35526)
                     /locus_tag="IW748_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1103020.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(35560..36021)
                     /locus_tag="IW748_000083"
     CDS             complement(35560..36021)
                     /locus_tag="IW748_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1103021.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(36151..36582)
                     /locus_tag="IW748_000084"
     CDS             complement(36151..36582)
                     /locus_tag="IW748_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1103022.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(36600..36920)
                     /locus_tag="IW748_000085"
     CDS             complement(36600..36920)
                     /locus_tag="IW748_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1103023.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            37219..37995
                     /locus_tag="IW748_000086"
     CDS             37219..37995
                     /locus_tag="IW748_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1103024.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            38010..38213
                     /locus_tag="IW748_000087"
     CDS             38010..38213
                     /locus_tag="IW748_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1103025.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            38229..38567
                     /locus_tag="IW748_000088"
     CDS             38229..38567
                     /locus_tag="IW748_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1103026.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            38554..38733
                     /locus_tag="IW748_000089"
     CDS             38554..38733
                     /locus_tag="IW748_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103027.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
BASE COUNT        12946 a         6597 c         7950 g        11470 t
ORIGIN      
        1 gtgacctgcc aaatgaattg aaagaagata ttgagattag gcgaaatatc actttttgga
       61 aaggtctggc taaagacttt ctttaaaaac agtggttcat gattcgtaat tgtgataagc
      121 ttctttttgg gcgtcaaaat aatagagagc gggtatgtat ccacttgaaa ataaccgcta
      181 ggacttgttt tagcaaaagg atattgtaac aataataacg ctggctttgt caactttgat
      241 tgattgaatt cttctgtacg ggaattttct gcatcgtcca atatggaagt gatgtaatct
      301 gacggaattt caaattgatg tgtcagctga tgaatctctt cttgattagg acgttctaca
      361 caataccaga tcgtttggtc agtttccttt ccggtattac gcagtttctc attatctaat
      421 gtaaaatatt tgatcatgat tttcctcctg ttgcctgttt cttttattat aacggatagg
      481 gaattggaat acatggaatg tgtttgtaag atatagaata taactgcata aatttaaata
      541 aaatgaaaca gttgtgtaaa gtatatacag tatacttgaa gcaacgataa aaaaggagtt
      601 tacgttcatg ggcacattca caccacttat cttgttttgg tatatttacc cggtaatcgt
      661 tttgtttggt tgccaatttc ttgtaaaatt attttcgtta aatcgtcgtt ttaaaataaa
      721 agcccctgat ttggcgattc cttttttgtg gggcggtatg catgcattgt ctagaaatac
      781 agggacaatt tctatcttac cgtttttctt tatttctgtt ttgctaatgg gaatttgtat
      841 tgctgttttc caagcatatt attatgaaga aattatttat ccgcgttatt tcaagatgac
      901 ttggcgtttg gtttttctgt tgacaatcgt cttgtacgct gtcttgatca ttgtgaacat
      961 actttcttac ctatgaaaaa aatagaaaag ctgtaaaaag tcttttaaaa ggctttttgc
     1021 agcttgattt ttaaaaaaat gtttatgaat aaaaaaagtt gttcccggat ttcccagaaa
     1081 aggtggtaga aagtggggga ttgtggtaga ctaattttag ttagtggagg aatggggggg
     1141 ctttcaatgt tgatgggtga attccaacat aatattgatg caaaaggacg tttaatcgtg
     1201 ccctcaaaac ttcgtgaaga acttggcgaa aagtttgttc tgacacgcgg attggatggc
     1261 tgtcttttcg gctatccgat gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg
     1321 ccgttggcta aaaaagacgc acgtactttt gtgcgattct tctattcggc tgccactgaa
     1381 tgcgaacttg ataaacaagg acgaatcaat atacccagca cgttaagaaa ttatgcagct
     1441 ttgacaaaag gttgcgtagt catcggtgta tctaaccgaa ttgaaatttg ggatgagaca
     1501 cgatggcaag atttttctgc agcagcagaa gagaactttg acgaaattgc tgaaagtatg
     1561 attgatttcg gactataaga ataggaagag gctgactaat gactgaaaca tttcaacact
     1621 atacagtcat gttgaaagaa acggtagatg gattgaacat caaaccggat ggcgtttatg
     1681 tagactgtac actcggcggt gccggacata gtgaatattt attaagccaa ctaagttcta
     1741 aagggcatct gtatgcgttt gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt
     1801 tagcttctta tgtcgaacaa ggtatggtca catttatcaa agcgaacttc agagaattag
     1861 aagaacgctt gagtgagcat gtagataagg tagacggcat tttgtatgat ctaggggttt
     1921 cctctcctca gctagatgaa gcagaacgtg gatttagtta tcatcaagat gcaccgttgg
     1981 atatgcgcat ggatcagtca gctcctttat ccgcttatca cgtaattaat gaatattcct
     2041 accatgaatt agtaaagatt ttcttccgct atggcgagga gaaattttcc aaacaaattg
     2101 ctcgtgaaat cgaacgagta cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg
     2161 aaatcattaa acaagtcatc ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa
     2221 gaatttttca agctgtgcgg attgcagtaa atgacgaact cggtgccgaa gaggcctctt
     2281 tagaacaggc aatccggtta ttgaaagtca atggaagaat cagcgtaatc acattccatt
     2341 cattggaaga cagaattgtc aaaagtatgt ttaaagaata tagtacggta caagatctac
     2401 caccgggact tcctgtcata cctgacgaat tccaaccaga actgaaagtc attactcgta
     2461 aaccaatcct tccttcagag gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt
     2521 tacgtattgc agaaaaaata agaatgaaag aggagtagaa tgatggcaga attaaagaaa
     2581 atgcaagatt acgaatatga tttacctgaa ttggtggatc agccggaaca acaaatgcct
     2641 gattcttctt cgaatcaaga aatcattctt ccacaatcac cggcaaaacg attaaagcag
     2701 atttctgctt tggaaaaaat tgctgtggca agcgtggcga ttgctattgt tgctttatgt
     2761 gttttaacag ttatgctgcg cacaaatatc agcggagctg aaaaagaaat cacgtcaata
     2821 caaacagaaa tcaatgataa aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa
     2881 ctttctcgta ctgaacggat taaacaaatc gcagaagaaa aaggattgtc tattaatgac
     2941 gacaatttaa ggaaagtgaa gtaaatgagt cttaaaaata aaattcggcg gtttatggaa
     3001 aagaaaaatc taaatccgat aaacaatcgc aaaaaagtag gtatcatttt atttgctacg
     3061 agtattggat tgttcttttt atttgccgtc cgtttctcgt atatcgttat tggaggtcat
     3121 gttgctggta cctcattagc tgaaaaaaca aaacagttgt accaaggaag tgaagtagta
     3181 aaagcaaaaa gaggaacgat ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc
     3241 tcttattcaa ttaaagcgat actctcaaaa acatatacct ctggggataa aaaactgtat
     3301 gttgaagaaa agaattttga taaaatagca gaaatcctgc ataagaatct ttctatagac
     3361 aaaaaagatg cactgaatat tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa
     3421 tttggtagct atgggaaaaa tatctcccaa gaaaccaagc aaaacatcga agcggacatg
     3481 aaaaaagaag gtgtcgctgg tctttatttt gtcgaccatc aggctcggat gtatccaaat
     3541 ggcgtctttt cttctcattt tattggatac gcagttccag acaaagatga gaatggctta
     3601 gttggtaaac taggtttgga gtctgcttac aatgatatct tgagcggtaa agatggtaag
     3661 attatttatc aaaaagataa ttttcaaaat cctctgccag gtacagtggc agaagaagaa
     3721 aaagctgtcg acggacaaga tatctacacg actttagaca gcagactaca aagttattta
     3781 gaaacactga tggatcaagt aaatgaagaa tatcagccag aagaattgac agcagtcctg
     3841 atgaaagcaa aaaccggaga aatcttggca atgggacaac gacctacttt caatcctgaa
     3901 acaatggaag gattgactgg aaaagatgct atctggcgta atttcttggt acaagatagt
     3961 tatgaaccag gatcaaccat gaaagttttc actactgcag ctgctatcga agaaggagaa
     4021 ttcaacgaga atgaaacctt ccaatctgga aaaatccaag tagaagatgc tacgatcaat
     4081 gaccatgact ttggtgaaaa aggtgtgctg accatgcgtc aagctctttc atggtcaagt
     4141 aacgtcggga tggtcatact ggaacagcga ttaggcggac gatggtataa ctatttacaa
     4201 aaactgggat ttggacaaag tactcattct ggattagatg atgaagtaaa cggtgcttta
     4261 cctacattaa atattgtcga ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg
     4321 aatttccaaa tgatgaaagc tttcacttct attgcgaata acggtacaat gattcaacct
     4381 cgatatatca gcaaagtggt ggatccccaa actggagaag agcgaacaac tcaaacagaa
     4441 gttctagggc aacctttttc aaaagaaaca acagaaaaag tccgtgaata tatgagagat
     4501 gttgtcgaaa gcgaaaacta cggtagtgct tatggtgtat atagtgtacc tggatacaat
     4561 gtatcggcaa aaaccggaac agcccaaatc gcttctgata cagggggata tcaaacagga
     4621 gatacagcct acctgtattc gattgtggag atggttcctt ctgaagatcc agactatgtc
     4681 ttgtatctaa caatgaaaca ccctaagaca tatgatcgaa tggcattggc taaaatcgca
     4741 aatccattga tgaaacgggc aatggatttt aaagaaactg aagaagattc agatacagaa
     4801 acaaaaacag aaaaagtatc cgtagccgat tatcgtaatc ttgaagcgga cgttgcagca
     4861 gctgatgctc aaaaaagtgg actgcaacct gtagtgatcg gtaatggtaa aaaagtacaa
     4921 aaacaatcta ctgccaacgg tgatcagttg atctctggag aaaaactgat cctatatact
     4981 ggtggtgata agctgatgcc tgacgtaaca ggatggtcaa aagcagatat catgaagctg
     5041 gggaaaatcc tcggtattga agttagtttt gatggagatg gttattgtgt caaacaagaa
     5101 cttgctccat atgaaaaaat cactgaagac aaacttagtt ttactttaga agaataagaa
     5161 gagaaatagg agagaaaaga gcatggaatg gacacaagca ttgattccca ttgtcagcag
     5221 ttgtgcaatg acaattgccg caatgccgct atttattggt tattttcaaa tgaaaaagca
     5281 aggtcaggcg attcgtgagg agggaccaaa gtggcacaat agcaaagccg gaactcctac
     5341 aatgggcggc ttagtgtttc tgatcggctc tattttgaca ggaatttggg ttggagcttg
     5401 gcaaaaacag ctaactccta ctcttttcat cttgcttttt gtattagcgt tatacggagt
     5461 aatcggcttt cttgatgatt ttataaaaat attcaagaaa agaaacatgg gactgaactc
     5521 gaagcagaaa ttgattggac agattctagg gggaatcatc ttttatcttg tttaccgttc
     5581 tgaaggttat ccaggtgtat tgaatttctt cggactagat ctacctttag gaatcattta
     5641 tggtattttc gctctcttct ggcttgttgg attttcaaat gcagtaaatc tgacagatgg
     5701 catcgatgga ttagttgctg gcttaggaag tatctctttt gcagcttacg gtctaattgc
     5761 ttggcatcag catcagtatg atgtattaat catttgctta agtgttttag gaggtttact
     5821 gggtttcttc gtctacaaca gaaaacctgc caagattttt atgggagatg tcggttcatt
     5881 agcgttgggc ggacttttag cggcaatttc tataatgcta aaccaagaat ggacattgct
     5941 tttgattggt ttgatctatg ttatggaaac agcaagtgtg atgcttcaag ttacttcctt
     6001 taagcttaca ggaaaacgaa tttttaaaat gtctccaatt catcatcact ttgaaatgtg
     6061 cggatggtcg gagtggaaga ttgatacagt tttttggttg atcagcattg tgacttcgtt
     6121 gatcacatta tggatcgttt ggtaggagat gacgggaaat gaaaaaaata tcaacatatg
     6181 aaaataagaa agtgcttgtc ttaggactcg caaaaagcgg agtaagtgcg gccaaattat
     6241 tacatgaatt gggcgcactt gtcaccgtta atgatggaaa accttttgac gaaaatccgg
     6301 aagcacaaga gctgctttct ttagggataa aagtaataac aggtagtcat ccaatcgaat
     6361 tgttggatga agaattttca ttgatggtca aaaatccagg gattccctac agtcatcctt
     6421 tagtagcaaa agcccaagaa atgggtattc cggtaattac ggaagttgag ctagcttacg
     6481 aagtagcgga atgcccaatt attgggatta caggaacgaa cggaaaaaca acgacaacaa
     6541 cgatgacagg actactactg aatgcaggag cagatcaagg aattgcacgt ttagcgggta
     6601 atattgggta tccagcaagt ggtgtagcgc aagaagcaaa atcagaagat aaaattgtga
     6661 tggaattgtc tagcttccaa ctcatgggaa tcacagattt ccgccctcat atagcggtga
     6721 ttaccaatat ctatgaagca catattgatt atcatggtac aagaaaagaa tatgtcaaag
     6781 ctaaatggaa ccttcaaaaa aacatgacag aaaaagatta tctgatcctt aactggaatc
     6841 agtcagaact acaagaatta gcgcaaagaa caaaagcacg cgtactgcct ttctcgacaa
     6901 aagaagtgtt ggaagacggt gtgtatgctg acgattactc aatatactat aaaaaagaaa
     6961 aaataatgga aatatctgaa cttggcgtac caggtaagca taatgtagaa aatgcactag
     7021 ctgctatttc tgtagcaaaa ttgtatggta tatcaaatga agctatacga gaaacgttgc
     7081 attttttcca tggcgtacca catcgtacgc aatatgtagg agaaatccaa ggcagaaaat
     7141 tttataatga ttcaaaagca accaatattt tagcgactaa aatggctcta agtggatttg
     7201 aaacgtctaa ggtcgtgtta ttggctggcg gattagacag aggaaataca tttgatgaat
     7261 taattccttc attaaaagga atcaaagcaa tggtcgtatt tggccaaaca aaagaaaagt
     7321 tgatggatgc tggaaaaaaa gcaggcatcg agacaatagt aacagccgat tctgtagaac
     7381 aagcagttcc tttagcatta gaaaacagta cagatggtga tgtagtttta ttatcaccag
     7441 cgaatgccag ctgggatcaa tacccaaatt ttgaaactag aggaaacaga ttcatggaag
     7501 ctgtgaaccg attaaaatag aaaaagtaga agcgagcgat aaaaaatgaa aattttggtt
     7561 acaggcggag gaacaggggg acatatttat ccagctcttg cctttgtcaa ttatgtaaag
     7621 actcaagaac cgaatgctga attcatgtat gtgggagcaa aacgaggtct agaaaacaaa
     7681 atcgtccctg atacaggaat gcctttccat accttggaga ttcaaggatt taaacgaaaa
     7741 atttccatgc ataacatcaa gacggtccga ttgtttctta agagtatccg tcaagcaaaa
     7801 aaaatcctca gtgattttca accagatatc gttatcggta ctggaggata tgtttccgga
     7861 gcggtagtgt acgctgcagc caaaatgggt attccaacag ttatccatga acaaaacagt
     7921 gtaccaggtg ttaccaataa atttttaagc cgatatgtgg atcggatcgc tctttcattt
     7981 aaagatgcgg cttccttttt tcctgaaaac aaagcagttc ttgtaggtaa tcctcgagca
     8041 caagaagtag cagatactaa aaaatcagat attttgaaaa catttgattt ggatccagaa
     8101 aagaaaacag tcttgatttt tggcggtagt caaggagcac ttaaaatcaa tcaagcagta
     8161 tcagtttttt taagtgaata tattccttct tcttaccaag ttttgtatgc ttcaggagat
     8221 agatattatg aagagatcct aaataaaata aatcctgaaa tacaaaatat tagtgtccag
     8281 ccttatatca aaaatatggc agaggtcatg gcttccagtg atctgctggt ggggcgtgct
     8341 ggtgcaactt cgatcgcaga attcacagct ctggggttac cggcagtgtt ggttcccagc
     8401 ccatatgtta cgaatgatca tcagactaaa aatgcaatga gtcttgttca cgcaggagca
     8461 gcaaaaatga ttgcagataa tgaactaaca ggtgaatcac taagtcagac agtcaacgaa
     8521 atcatgggtg atgaagaatt gcaaaagcaa atgtgccgag cttctaaaga acaaggaatc
     8581 cctgatgctt caaaacgtct ttatgactta gtaaaacaaa taatcaaaaa ataaaggcgg
     8641 tggtcttagt gagtaagaaa aaagaaccaa aaaaattgtc tcatccgtct tctgatgaaa
     8701 acaatttgac tccttggcaa aaggctaacc gaaaatatct ggcagaacaa gagatgaaaa
     8761 accaagagcc aaaaaaagaa gacataacat tagatacacc cctttcagat tctgaagaag
     8821 aaaaaacggt tcaagaggaa cagcaagaag aaagccagtc cgagaaaaaa atcgaatttt
     8881 ttgaagagat caagcaaaag aaaaacggtg gaccatataa cggttctttt ttgaaccggc
     8941 tgcccaattt aaaaagtcaa cggcacaaag tactatttcg tcggttagct ttgatcatca
     9001 ctgtattggg tataccgctg atttttttga tctattatgt ttctccttat agcaaattac
     9061 aagcagttgc agtatcaggc aataagacag tcaacagcca agaaatcatc tctgatacaa
     9121 aattgtcatt aggagaaaat gtttggggac agtattttca tcgatctact tatatcgatc
     9181 gccttaaaaa agcacagcca agaatcgaaa cagcaaatat acatttcaaa gggatgaatg
     9241 aattcgattt ggatgtaaca gaatataaag agattgcttt gatcgcaaaa aacaatcaat
     9301 actacccagt gattgaaaat ggaacagtat tagatgaaaa agtagcaaat cctacaaaaa
     9361 atctccctat cttggaagag ttcaaagact ctgcaaaaat caaagaatta acaaagcaat
     9421 acaatcagtt atcatcagaa ttacaaaaag caatttctga aatcaagtac acgccaagag
     9481 caagcaataa aaatctgatc caattgaata tgaatgacgg caatcaagtt attgtaaata
     9541 tcaataatct tgcgaaccaa atgaaatatt attcgcaagt agccaaagat atggatgaaa
     9601 aaggtgtgat cgacatggaa gttggtatct tctcgcatcc ttacggtagc actcaagaaa
     9661 cggaagaatc gggccaatcg gaagaaacaa ctgaaagtga agaaaatgcg gatcaaacaa
     9721 acgaatcaag tgaagaggca gaaacgcaaa aagcacaaac acctggatcg tctagcacaa
     9781 acggatcatc tgattcaaat gattcaagtg aaacaagtca aatagaagat aatccaagta
     9841 caactccgtc aagtttaaat gaaaattgat aaaacaactt taaaaagtaa gaatgtactt
     9901 tctaaaaacc agtaatttat ggtagaattg gaagcagtga atatatctat taataagtat
     9961 attttaaaac ggacgctagt tgttcgcttg tatatggaat agtaagtagg agggaatccc
    10021 attcatggca aaaacaggaa tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt
    10081 tgtcgtggct gaatatatcg acagccaaat gaatattatt ggagtaggaa acgcaaaatc
    10141 agaagggatt aaccgaggca ttatcgttga tatcgataaa acggtccaag cgatacaacg
    10201 agcagtacga caagcagaag aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt
    10261 gccagcaaac atgcttgaag tagaaaattg ccagggaatg attgcagtta acggagattc
    10321 aaaggaaatc accgatgaag atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat
    10381 acctcctgaa cgtcaaatcg tgtcaatatt gccacaagat tttactgtcg atgggttcga
    10441 gggaatcaaa gatcctcgtg gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt
    10501 tacaggacct aaaacgatca tccataacat acgtaaatgc gtggaaaatg ccggattaat
    10561 cgtcaacgaa atggtgatta ctcctttagc gctgactgaa tcaattcttt ctgacggaga
    10621 aaaagatttt ggtacgatcg tcattgatat gggtggcggc caaacaacaa cggctgttat
    10681 gcatgataag caattgaagt ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa
    10741 agatatctct attgtattaa atacgtcatt taacaatgca gaagcgttga aaataaacta
    10801 cggggatgca tacccagaac ggacatcacc agatgaagaa tttccagtag atgtgattgg
    10861 tcagtcagaa ccagtgaaag tcgatgaacg ctacttgtca gaaattattt cagctcgaat
    10921 ggaacagatt ttcaacaaag caaaagatgc attggaccaa atcgaagcat tagagttgcc
    10981 aggtggcgtc gtattgacag gcggagctgc tagtcttcct ggtgttgtgg acttggcaca
    11041 agaaatcttc ggcgtaaatg taaaattgta tgttccaaat cacatggggc tgagaaaccc
    11101 ggtatttaca aatgtaataa gtatcgtgga ttattcagca aacttgagtg aagtgtatca
    11161 attggcaaaa attgctgtta cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca
    11221 agaaacaaca aacacatatg agagctatga agcacctgaa gaaacaatct acgatgagcc
    11281 ggaaccaaaa gagtccggag aaaatgtaaa aaataaaatc aagggtttct ttacaaatat
    11341 ttttgattaa ttcatcctgg gaggaataag atatggaatt ctcaattgat aataatatca
    11401 atgacggcgc agtaatcaaa gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc
    11461 gaatgattga agaaaacgtt aaaggtgttg aattcattac agccaacaca gacgtgcaag
    11521 cattaaaaaa ttcaaaagct gaaacagtta tccaattagg ccccaaatat actcgtggtt
    11581 taggtgctgg ttctcagcca gaagttggac aaaaagcggc agaagaaagt gaacaatcct
    11641 tacgcgaagc tttagacggt gcagatatga ttttcatcac tgcaggaatg ggtggaggaa
    11701 ccggtacagg tgctgcacca atcgttgcag gtatcgcgaa agaattgggc gcattgactg
    11761 ttggtgtcgt gacacgtcca ttcacttttg aaggaccgaa acgtggccgt tttgctgcag
    11821 aaggtatcgc tcgtttgaaa gaaaatgtgg atacattatt aattatctca aataaccgtt
    11881 tattagaagt tgtagataaa aagacaccga tgcttgaagc tttccgtgaa gcagataatg
    11941 ttttacgaca aggtgtacaa ggtatctctg atttgatcac agcgccaggt tacgttaacc
    12001 ttgactttgc tgacgtgaaa acagtgatgg aaaaccaagg tacagcattg atggggatcg
    12061 gtgtggctag tggagaagat cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat
    12121 tgcttgaaac atctattgat ggagctgaac aagtacttct taacatcaca ggcggtttgg
    12181 atatgacatt gttcgaagca caagatgctt cagatatcgt agcaaatgca gcaactggcg
    12241 atgtaaatat cattctaggt acatctatca atgaagaaat aggcgatgaa atccgtgtga
    12301 cggtcatcgc aacaggtatc gatgaatcaa aaaaggaacg caagtctagc cgaccagcta
    12361 gacaagcaca aatgcaatca cctgctcaaa agactgtatt agatatggat caagctaaac
    12421 caacttcttc agaagaagaa aacagttttg gcgattggga tatcagacgt gaacaaaatg
    12481 ttcgtcctag agttgatgat tccaattttg acaatatcga aaaaaaagaa tttgatacat
    12541 tcaaccgcga agaaacaaaa tctcatggcg atgatgaatt aaatacgcca ccgtttttcc
    12601 gtcgtaaaaa ataaaggaga ggaagagcac atgattgctg acaacttgca gaaaatcaag
    12661 caagaaattc aggattcgtg tgctcttgtt catcgtgatc cgaaagaagt tacattagta
    12721 gctgtaacaa aatctgttga atccgctcaa gctgaagaat tggttagagc tggcgttatg
    12781 aattgtgcag aaaaccgagt agataaatta ttagaaaaga aacaagattt atttgatttt
    12841 cctgaaataa agtggcattt aattggtaat ttacagcgca gaaaggtaaa attgattgta
    12901 aatgaaattg attactttca tgcgttggac agtttaagat tagcagaaga aatccagaag
    12961 cggttagaga aagaattatc ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa
    13021 catggaattt caccagatga gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa
    13081 atcaaggttg ttgggttgat gactatggcg cctaaagaag cttctgatga gcaaatccat
    13141 aaagtgtttg gtacgctgaa agaattgcgt gatcttgttc aagcaaaagg ttatgcccat
    13201 gcaccatgta gtgaattaag tatggggatg agtcaagact ttcaaattgc gattgaagaa
    13261 ggtgcaacat ttatccgtgt aggaacagcg ctattcaaag acgaataaag gaggaacaaa
    13321 aatgccgctt ttaaacagag gagcaattgc taattttttc ggattagctg atgaggaaga
    13381 gtacgaatac caagattatc ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa
    13441 tacaacacag aatcaatcaa ctcattctgt atctgctgcg aatactcatg aaaaacaaaa
    13501 aacggcgact tcacgtcctg taacgcatga aagtattcag ccggctgaaa agaaaaaagt
    13561 aattgaacta catcaatcac ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac
    13621 ctctacacaa cctaaagcaa atcttgctgg aaaaatcact gtaatggaac caagagctta
    13681 ttctgaagct atggcgattg ctaaacgaat cattgctgga gaagcaacct tagtgaattt
    13741 tcatttagta gaagaaaaac aagcgagaag aatcgttgat tttctaacag gaacagttta
    13801 tgcactagat ggagatatca aacgtgttgg agatgagatt tttctttgta caccgactgg
    13861 gatggagatt gacagttcaa ccgctcaatc ttttgcagat gcgaatttgt ttgatctgta
    13921 agaggaggaa ctacgattta tactattgca ctattacttc atttacttca acgggcagcc
    13981 tatttctata gcattatctt gatggtatat gcattgctct cttggtttcc aggcgggtat
    14041 caatcagggc taggacgatt attagctaag atctgcgaac cgtatatcag tttgttcgat
    14101 cgtctgcctt tgaggattgg acccttggat ttcacaattt taggggcaat cctgatcttg
    14161 aacttagccg tacaaggttt agcacagatc gttatatgga taactgtcca tttagtttat
    14221 tgattgaagc aacaaaatct ttattctcag aatagatagg aggggaaaat tttggatgca
    14281 aatgtatacc agcactttcg ggcagatgaa cgtacattta tcgattcggt aggcgattgg
    14341 attgaacagg ttcaaggaca atatgcaccc tatctgaccg aatttcttga cccaagacaa
    14401 tcctatattt tagaaacact gattcgacaa agcagtgatt tgcgtttcat gttttatggc
    14461 ggatatgaac aggcagaaag aaaaagatgt ttgattttcc ccgattatta cgaacctgta
    14521 gaggaagatt ttgaaacagc attgtatgaa atccattatc ctagcaaatt tgccagccta
    14581 agtcatggga aaatccttgg aactttagtg ggaacgggga tcaaacgctc ttactttggc
    14641 gatatcatct cggacggtga tcgctggcaa gtatttattg caaatgaggt ggatcatttt
    14701 gttgctgcac aagtaacaaa gattggaaag gttgctgtac gcttggaacc aatcaaatac
    14761 acagaaatga ttatgccgaa agatagctgg acacaagaaa gaggaacagt tagttccatg
    14821 cgactagaca gtgtgatctc tgagatttat aatatttcca gacagcggtc caagcagctg
    14881 atcgaagctg gtaaagtaaa agtgaactgg acagaaaata caagacctga ttttgtactt
    14941 gaactattag atattatttc agtcagaggt tatggcagag tccagatcca agaacttgag
    15001 gggaagacga aaaaagaaaa gtacagggta ctgttcggtg tattacgtaa ataaaataaa
    15061 aaggtggttt actcatggct ttaactccat tagatatcca aaataagaca ttcccgacaa
    15121 aaatgcgggg atataatcaa gacgaagtag atgatttctt agatttagtt gttcgtgact
    15181 atgaggaatt gactcaaaga aatcgagaat tagaaaaagc agtcaaacat tctgaagaaa
    15241 aacttgagta cttcaatgaa ttaaaagatg ctttgaacca atcaatcatc gtagctcaag
    15301 atacggctga taaagtaaaa acaagtgcaa gcaaggaatc cgaagtgatc gttacttctg
    15361 cacaaaacaa agcagatgag ttagttgcga acgctgaaaa acgtgcacat caattaacaa
    15421 ctgatgcaga agaaaaagca agaaaaatct tgacagatgc aacagaaaaa gcaagacagc
    15481 ttgcgacaga aactgaagat cttaagaaaa aaacacgagt attccatcaa cgtatcagct
    15541 tgatgcttga gtctcaatta gaacaagtga aaagtccgga atgggatgaa attttacaac
    15601 cattctccag ctacgtgact gacagccatg aagtgatcaa agaagtttta gcaaaacagc
    15661 ttgacaatga aaatgatact gatgtaaact cagacacagg agtcatagaa gcacctatga
    15721 cggcatttga tacacaggct attgatatct cgatcgtgcc tggtgaaaat gaaaacgagt
    15781 agaacagaaa agcactttgt tattcttaaa gcgagccgat gagagtggaa gttcggtaag
    15841 acactgcatg gtgaaagatc ctctacttga gacctgcttt tccgaaatta gagtaagaag
    15901 cagcggctga cctcgttatt ggtcacagag agtttgcact agatagatgc aactaaattt
    15961 gggtggtacc acggcatgtt cgtcccttgc ggctgaacat gcttttttgt gtccggaaaa
    16021 caaataaagg agtgtatggt tgaatgaaaa tgaaagaaac cttgcaatta ggaaaaacaa
    16081 aattccctat gcgtggtaat ttgccaaacc gagaaggcga gtggcaaaaa gaatgggaag
    16141 aaaatcgaat ctaccaaaaa agacaagagt tgaacgaagg aaaacctagc tttgttcttc
    16201 atgatggacc tccatatgcc aatggtaata tccatttagg acattcattg aataaaatca
    16261 gtaaagacat tatcattcgt tcaaaatcaa tgtctggcta tcgtgcacca tatgttccag
    16321 gatgggatac acatggattg ccgattgaac aagtattggc taataaaggc gtgaaacgta
    16381 aagaaatgtc catggcagaa tacttggaaa aatgtcgtga atatgcactt tcccaagtag
    16441 ataagcaacg agcagatttc aaacgcctag gagttgcagg agattgggaa catccttatg
    16501 ttacattaga tccttcttat gaagctgcac aagtacgcgt atttggtaaa atggctgaaa
    16561 aaggctatat ttacaaagga ttgaaaccaa tttactggtc tccgtcaagt gagtcttctt
    16621 tagcggaagc tgaaattgaa tataaagatg taaaatctcc atctattttt gtagctttca
    16681 atgtcaaaga cggcaaagat attcttgatg aagatacagc ttttgtcatt tggacgacaa
    16741 caccatggac attgccagca aaccaaggta ttgctgtaaa tccaaaattt acgtatgtat
    16801 tggtggaagc cgatggccgt aagtttgtcg tagcgaaaga tttgctagaa acagtccaat
    16861 cagaaatcgg ttgggaaaat gtaacagttc taaaagaatt tgctggacaa gaaatggaat
    16921 atatgactgc aagtcatcca ttctatgaac gtgaatcact tgtgatttta ggcgatcatg
    16981 ttactttaga cgctggtact ggattagtcc atactgctcc aggacatggg gaagatgact
    17041 atatcgctgg tagcaaatat aaactatctg tagtgtcacc tgtagactca aaaggattgt
    17101 ttacagaaga agcacctgga tttgaaggta tcttttatga caaagcaaat cctatgatca
    17161 ctgatctatt aaaagaaaaa ggagctttgc tgaagctaga tttctttact catagttatc
    17221 ctcacgattg gcggacgaaa aagcctgtga tctatcgggc aactccacaa tggtttgcat
    17281 ctatcgataa attccgtcag aatattttgg atgaagtaga aaaagtagag tgggttatcc
    17341 catggggtaa aacacgtttg tataatatga tccgtgaccg cggcgattgg gtaatttccc
    17401 gtcagcgtgc atggggagta ccacttccga ttttctatgc tgaaaatgga gaagcaatca
    17461 tcacaccaga aacaatcgaa catgtggcaa aattatttgc tgaacatgga tcaattatct
    17521 ggtttgaaaa agaagcgaaa gaacttcttc ctgaaggatt tacccaccca ggttctccta
    17581 atggcgaatt tacaaaagaa aaagatatca tggatgtctg gtttgattct ggttcttctc
    17641 atgaagctgt tttacgtcag cgaccagaat tatcattccc agcagatatg tatttagaag
    17701 gatcagacca atatcgtggc tggttcaact caagtatcac gactagtgtg gctatcaatg
    17761 gtgtagcacc atacaaatca gttatttctc aaggatttgc tttagatggt gaaggccgta
    17821 agatgagtaa atctttagga aacatcattg caccagaaaa agtgatcaaa caatttggtg
    17881 cagatatcct tcgcttatgg gtatctagtg tagattatga agcagatgtt cgtgtttcta
    17941 tggatatctt aagtcaagtg gcagaagtat atcgtaaaat cagaaatacg atgcgtttct
    18001 tattagcgaa tacagaagat ttcgatccta aaaaagatac tgtttcgtac aatgatttac
    18061 gttctgtaga caaatatatg accgttcgat tgaaccaagt aatcaaagaa atccgagaag
    18121 aagggtacga caaatataac ttcatgcata tttaccgtac agttatgaac ttcttgacgg
    18181 ttgatctatc gtcattctac ctggattttg ctaaagatgt agtttatatt gaagcagaag
    18241 atgattatca acgtcgttgc atgcaaactg ttttctatca aacagctgta gctttgacaa
    18301 aattattgac tcctatcatt ccgcatacag cagaagaaat ctggtcattc ctgaaagaag
    18361 aagaggagta cgttcagtta agcgagtttc ctggttatga agagtttgca aaccaagaag
    18421 aattgatgga tacctggact gctttcatgg acttccgtga taatgtctta aaagcattgg
    18481 aagaagcaag aaacagcaaa ttgattggta aatctttgga agcaaaacta actgtttatc
    18541 caaaacaaca agttcgtgaa atgttaaaag ctttggatgc agatattgca caattattga
    18601 tcgtttcacc agactacttt gaagttatgg cagcagatga acaagtgcca gataacgcta
    18661 tggtatttga tgatgttgct attacagtgg aaaaagcaga cggggaaact tgcgatcgct
    18721 gtcgccaagt tcgtaaagat gtaggagtag atgaaaaact tcctcatcta tgcggtcgtt
    18781 gtgcaaaaat cgttgaagac ttctatcctg aagctgtagc tgaaggattc gaagaaaagt
    18841 aaaagggaaa agggggtgtg acatgttctt tgtcacaccc ccttttgtta tcaatcgttt
    18901 aattttccac gttcacgata ccagttatct ggttgccaga tccattcgaa tccgtctttt
    18961 tcaagtagtt cgaaagctgc tttaggtccc attgatccag ctttgtagtt gggaaaatcc
    19021 ggttgtgttt ggtcccaagc ttttcgaatg acatccacga tatgccaaga ttgagcgact
    19081 tcttcccaat gagagaagtt cgttccatca ccattcaacg catctaatag taatttttcg
    19141 taagcttctg ggctattttc aaccatttct gcactatttc taaattccaa tttgattggt
    19201 tccgtttcaa atccttgccc tacttctttc ccattcaagg ataaagaaaa tccttcagtt
    19261 ggctgaatat agatcgttag aacattaggt ggaagtgtcg tatcgtcaca tggttcatcg
    19321 acagatgttt tgaatacatt aacaggaact tgtttgaata cgatgttgat ccgagtccct
    19381 ttttcagtca aacgtttgcc tgtacgtaca tagaaaggaa cacctgacca acggaagtta
    19441 tcgatagtga atttgcctgc gacgaatgtt tcagtctctg attgagcatc cactttgtct
    19501 tcttcgcgat agcccttgaa ttgtacacca tccaattcac cttgagcata ttgcccgcga
    19561 acgaagtttt ctaacacttc ttcttcgtta taaatgcgga tagctttcaa tgctttgatc
    19621 ttttctgtac ggatttcttt ttcggaaaat gctactggcg gttccattgc aagtaatgct
    19681 acaacctgta aaatatgatt ttgcaccatg tctttaagtg cgccactatg atcgtagtag
    19741 cccccgcgat cttctacacc gaggttttcg ccaaaagtaa tttggatatt gtcgatatag
    19801 cgattgttcc attgagcctc aaaaatatta ttggcaaaac ggatagctga gatgttttgg
    19861 atcatttctt ttcctaaata atgatcaatc ctgaaaatat cttgttctgg gaatacttct
    19921 ctgatttgct ggttcaattc aaaagcagac tggtaatcag aaccgaacgg tttttctatg
    19981 atcaaacgat tgaagccatt ttttgtcata atattttgtg atttcaaatg agaaacgatc
    20041 gttccaaaaa attgaggtgc catagccaaa taatagacac ggttaccgtt taattgatag
    20101 tgtccatcta atcggtcaga caattctttt aatgtctgat aatgcgttga atcatttaca
    20161 tcatgggatt gatagtaaaa atggcttgca aaggtagttg cttcttcatc tgaaggattc
    20221 aatgcacgga ttgtttcttt gacaacttca cggtagtgct catcgctcca gggacggcga
    20281 gcggttccga ttacagcaaa atgctcgcct aaatttcctt ttctgtaaag gcggaacaaa
    20341 gacgggtaca atttacgctg ggctaggtca cctgtaccgc cgaatatcgt aaacaataca
    20401 tttttttcat tagacatgtg gatacactcc tttatatttt atcgacaaaa atcgtgctgg
    20461 ccgccttaaa gctgacgggg attttctttc tatcattttg gatagtgatc ggtccttcat
    20521 aagctgcgat gtcaacgatc tcatattctt cgttgatatt caggtcaata gtgactaagt
    20581 aatccaacaa ttctttttca tcaagcactc gtgcgatacg cacttttgta ccgacaggat
    20641 aggactccaa tgtttgtcgg cgttcttcat ggacaacttc ctctttttta ggaatcgatc
    20701 ctccatgagg acaatgttca ggatgattca aatagttttc taaatgatct gcaagatgtt
    20761 ccgaagtcac atgttccaat acttcagcat catcatggac ttcattccag ctatagtcta
    20821 aatgttcaac taaaaaaact tcccataagc gatgtttgcg aatcagactg ctagcttttt
    20881 gcagtccgct tgcagtcaat tgtacacctt gataagggga atgttctaca agctcttctt
    20941 tcacaagttt agaaatcatt tcactgacag atgcagctga gacatctaga ctagatacga
    21001 tttgtttatt gttgactttc gttgtatctc cacctagttc taaaatgatt ttcaaataat
    21061 cttctcgatt tggtgtcata ttcgatcatc ctttcatggt aaaaaaccat cattagtaag
    21121 tgtaacaaaa aaaacgttaa ttgactattt ttctaaataa aaaacaaagg atttttagac
    21181 gtttttacaa caaaaaacga aagatgatag agaagactca ttctttcgtt tataaaggat
    21241 atatatatac attgggaggg atctattttt cagataacaa cttatatgaa gtgtctgcag
    21301 attcgtcata aagagtttgt tcgggataag aaaagataag ataaaaacca tactgatcac
    21361 gtacgactaa acgatattca tctatttcta ccacagatgt gccaagagac tgaccgttga
    21421 tcaaaaacgt gtaatcagat gtaatctgta attgttgttt ttgctggcta tcactaaaga
    21481 accatgtacc ttgatacttt gagagtcctt ctgacttttc cgcttgtttt ttctttttca
    21541 agtaaataaa acttgagaga acggtaatga gtgaaccaag aaaatagatc atttttttta
    21601 tcaccaaatc actcctttat gagaaaataa tcgcatagac atattctttc ttctttattt
    21661 cattgtaccg aataaaagct aaaatttctc taagactttc gactggtttt tttactgttt
    21721 ttctacaaat tatgatagga tacatagtag gaagaaaggt gacataattt gaagtatcag
    21781 caagtggaaa tagctttttt tattgaccgc ccgaatcggt ttatagcaca ttgtatgaaa
    21841 aaagacggag aaataattat tgcgcatgtc aaaaatacag gtagaggaaa agaggtcctg
    21901 ataccaggcg ctgaagtggc tattgtattt gctcctggac ctaaacggaa aaccgcctat
    21961 gatctgattg ctgtaaaaaa gaagggtgac tggtttaata tcgatagtca attgcctaat
    22021 cggctggcaa ttgatggtat tttagacggc acgatccttt taccaggatt aactggtcca
    22081 tttacggtat acaagcgtga agtaacttat cttcactcca aatttgatat ttatttagag
    22141 acggtggtcg gacaaaaagc atttgttgaa gtcaaaggta tgacacttga aaataaagcg
    22201 attggtgctt ttcctgatgc gcccacactc agaggattga aacatatagg agaattaacg
    22261 tatgccgcac aagatggtta tgccgcttat gtcttgttta ttgctcagtt cgaacatctt
    22321 catctggcaa cgatccatga agaaatgcaa ccagcactag ctgatatggt tcgacatgcg
    22381 cagcaaagcg gagttcaaat cctcgcctat aattgtcaag tatcggcaga tgaggtaact
    22441 gtcaagcagg ccattccatt tgatgtagat atcccttttg aagatcctat acaatagaaa
    22501 gaaggtaaaa taatgatcaa cttaggtatt atcggaacaa attggattac tcatcaattt
    22561 gtccaagcag ctttaagcac aaaaaaatat gaattgacag cggtatattc tagacatttg
    22621 gaaaaagcac aggaatttgg ttctcattat gaaggggata tcgaattcgc aacagatttg
    22681 aagacttttt tcaatattac tcatatggac accatttata ttgcttctcc gaatagtctt
    22741 cactttagtc aagctaagca agcaattttg gcaggaaaaa atgtgatagt tgaaaagcca
    22801 gctttttcga cgccaaaaga aatggatgaa atcatccatt tggcaaatga acacaatgtg
    22861 tatttttttg aagcagcaag aaatatccat gaatcaggtt ttaaaaagat tgccgattac
    22921 ttaccattaa aagacgagat aatcggtgca aatttcagct atatgaaata ttcttcgcgc
    22981 tatgaccaag tattagatgg agaagaaccg aatatcttct cgactcattt ctcaggtggt
    23041 gcattgatgg atttaggtgt ttaccttgta tatgcagctg ttgcttggtt tggaatgcca
    23101 aaagaagtcc attatttccc aagaaagatt tctactggtg tagatggtct tggatggggg
    23161 atcttacgtt atgagaattt tgatgtatcg atccagccag gtaaaatcgg tgattcttat
    23221 ttaccgtcag agatttattt tgactcagga acgctagtga tgaatggagt caatgcaatt
    23281 gaacaagcac aatttcatga tcgcgcgcat aaagaaatca ttgatttggc ggtaacagct
    23341 gcagaaaatc caatggcaga tgaagcagag gattttgcca atgtcatggc tcatccgaaa
    23401 gatccagctt ggggattgtt gtatgaagag tggatcgagc tagctagaaa tgtgaaccaa
    23461 gtgatttatg atttaagaaa aaatggcggc atcaaattcg atgcagataa tgaggaagat
    23521 ttttaatgaa cgaatttgta caagtatttc ccgaaaattg gcagaaaaaa tatgaggata
    23581 aagggtttat ctcaccttct cttattcaac gagcagtttt tcagccacta agtaataaac
    23641 aaagtatagt agcggtttct cctacaggtt caggtaaaac actggcttat ctttggcctc
    23701 tactactaaa cgtggaacca ggtgaagcaa gtgcgttggt catttttgct tcttctcaag
    23761 aactagctat tcaagtagca gatgttgcac gtgaatgggg aaaagacaaa gagttgaaag
    23821 tccaatcttt ggtgggcgga gccaacgtca aacgtcagat tgagggtttg aaggaaaaac
    23881 cagaaatctt gattggtaca ccaggaagaa tattggaatt gatgaaagca aaaaaaataa
    23941 aagcacatca agtgaaaaca atggtatttg atgaagcaga ccagctattt gacgctggga
    24001 acagccaaat cattgatcaa attcttcatc aagctccaac agagtatcag ctagcttttt
    24061 tttcagcgac agcagatcgt tcccttgaaa gcattgaaaa aatcactggg aaaacgtttg
    24121 agacaattga cgtgacagca gaggacgatt ctcgaaaagg actgagacat tactttttac
    24181 gtgtaccgat acgaaagaaa gatgactact tgagacgtct cacgcatata gaagattttc
    24241 atggtctgat tttctttaat caactgactg aattaggcgt aatggaagaa aaattgctgt
    24301 atagaggtgt accggtagga agtctggcct ctgatcagaa caagctactc cgaaaagcag
    24361 cgctagaaca attcaaaaag aaaatggtca gagcactttt gaccacagat gtggcggcta
    24421 gaggtttaga tattacaggt cttccttatg tagtgaatgc agaagttccg ttaagtgaag
    24481 aagcttattt gcatcgttcg ggaagaactg gtcgaatggg taatgaagga atagtaatta
    24541 cactcgtaca agataataat ctaagagatt taaaacggct tttacgtcca tcaaatataa
    24601 cacttgaaga gattttttta tacggaggta agctgcaaac tgagcagcca accaaagaag
    24661 atgcagctcc tgcagtaaat aaaaagtatg cgaaaacttt ttctggaaag gagcagaacc
    24721 cagaatataa caaagaaaaa ataagaaaaa aagttaaaaa taaatcaaaa aaagagagaa
    24781 ataaaggagc aaggcgcaaa taagcgcctt tttatgctat gattatgaaa acgggaacaa
    24841 aattggttgc cagaggaggg gttatgtgaa agaacgtcaa gaagagatct tgcagctatt
    24901 gacagaaaac caacaaggat tgacagcaag tgatgtagct gagcgcttaa ctatcgatcg
    24961 aagcaatgca agccgatatt taagtgaact atacaaggcc catcatattg taaaaaccgc
    25021 tggccgacca gtcgtttact ctttgccaac ggaaaagtca aaatctgatg aagtgcatgt
    25081 cgactcttct acgcaagtga cttttgaaac actcgttgga gaaaatgatt ctttaaaagt
    25141 cagcatacag caggcaaaag ctgcgattct ttatccaccg cgggggttgc atacgattat
    25201 ttttggagaa actggtacag ggaagtcgat gtttgctgaa tgtatgtatc attttgcaat
    25261 cgattcagag atgctttctg cagatgcgcc atttgtttct ttcaattgtg cggattatgc
    25321 tcaaaatccg caactgctct ttggacacat ctttgggatc aaaaaagggg cttacacagg
    25381 agcagctcaa gatagtcccg gtctaatagc caaagcagac ggcggtatat tgtttttaga
    25441 tgagatccac cgtttaccgc cggaagggca ggaaatgctg tttactttca ttgataaagg
    25501 tatttaccgt ccgcttggag aaagtgcaca agtacatgaa gcatctgttc agatcattgg
    25561 ggcgacgact gaatcttcag aaagtttctt atctacgttc aatcgacgga ttcctatggc
    25621 aatcaccttg ccgaatcttg cttcacgttc actggatgag cgttatgaaa tcatctctct
    25681 ttttatcaaa caagaagcga atcgtttgaa tcaacgtatc gatatcgaaa aagaagcgat
    25741 tcttgccttc atgctttatg atgcggaagg aaatatcggt caagtgaaac gagacttgaa
    25801 actagtctgt gcaaaatctt ttttacatta ccgtactcat catgagaaaa agctaatcat
    25861 tcgaaaagaa gaactttccc ttcaagtaca aaaaggatta ctgaaaatca aggaaatacc
    25921 ggaacgtctc gaccgattca tggacagcaa aagtcagtat ttgacatttg aaccaggatt
    25981 tgctgatgtt gtctggtcac aagatccaga acgaaacatg caggtctata acgatattga
    26041 ggaaaaagtc acttctcttt ccgaaaccgg tgtggagaac attgatctag agaccctcat
    26101 ttcaaaagat gtggatgctt atttccagac atatgtcaaa gaattgacta aaagttcgat
    26161 tcccaaagat cttttgccag atgaaatctg gcggttaacc aatcaattgt atgatgtggc
    26221 tgaaaaacgg ttagaccgta tttataatga aaaagctcgt tttgcttttg ctttgcatct
    26281 gcaaagtaca ttggatcgtg tgagagaagg tcatatgatc gtccacccag atttgaataa
    26341 cgtacggaaa aattttaaga gggaattcca ggtagcaatc gatctttcaa cgatcattga
    26401 agaagaagaa caagttgaga ttccttttga tgaaatcggc tttattagta tgtttctttc
    26461 tatcaatcta ggagaaagtg agcctgttaa agaaaacaaa gtagatgttc tcatattgat
    26521 gcacggcaga cagactgctt caagcatgtt ggagatggca caagaactat taggaacaaa
    26581 agtagggaaa gcttttaata tgccactggt tatggaagtt caaacaatgt atgaagaagt
    26641 gttacaatat gtcaaaaact atcaagagca tttgacgaat ggcctactct tgttaacaga
    26701 tatgggatca ttaaacacct tcagtgattt gatttatgaa gagactggca ttcggacgaa
    26761 agctatcacg atgacaagta ccatgattgt gatagaagcg atacggatgg caagtgttgg
    26821 aagaagctta gaagatattt atcaaaatat ccagctttcc tttgaatcga tcgtacgtga
    26881 tcagttcaaa gcggaaaagt ctgaaacttt acagcgtaaa aaagccgtaa tcgtgacatg
    26941 ctttactggt gaaggagtag ctgccaaatt atatcagcgt attatgccag tgattaatca
    27001 aaataaggta gaactgatcc agatgcagtt tttagaacgt gaatcattca aaaaacacat
    27061 cgatgcctta ttggaagaat atgagatcaa agcaattgcg ggaacagttg atgttgatta
    27121 tcaaaatatc ccattttttt cagcctatga cgtttttgat gatgaaaaac taaatatttt
    27181 aaagcgtata gctgatgatg agatcccgac agctaaaatt gtcgattcac ttgctggatc
    27241 attaagcgat gtcttttcta ttgatgaact gatccgttca ttgcaaaaga cagttcagca
    27301 gatccagacc gatttgtcga ttatcgtgga acctggagta gatactggaa tcgttatcca
    27361 tttagccttc atgttagacg gattgatcaa acaagaaagc catcggcaat ttgagaaact
    27421 gtctattttc tcaaaaattt atcgtttgga aatggatatc gttcgggcaa acttgatggc
    27481 actagaacga aaatatcagg taaaaattcc agaagatgat atcgcatttc ttacacaaat
    27541 gtttcttgaa aataaaataa aaaaatgaac acactaatca tactgtttac acactaaaca
    27601 tctgtgtgcg cactataaaa aagagctaaa aagcatgaaa acgctatttc agctcttttt
    27661 tttattggca tgcttcttgc gttatataaa agtgtaaaaa aataaaagga gtgttaaaaa
    27721 tggcaaaaaa aacaatcatg ttagtttgtt cagcagggat gagtacgagt ttattggtca
    27781 caaaaatgca aaaagcagca gaagaaaaag ggatggaagc cgatattttt gcggtctctg
    27841 cctcagatgc agataataat ttagaatcta agaatgtcga tgtattatta ttaggtcctc
    27901 aagtacgttt catgaaagct caatttgaac aaaaacttgc gcctaaaggt attccattag
    27961 atgtcatcaa tatgcaagac tatggcatga tgaacggaga aaaagttctt gcacaagcag
    28021 aaaatctaat gaaataaatc aagtatgcag ttgggggtat aacaacgtgg aagatcaaca
    28081 aaatctagaa gccatcatgg ggttaatcat gtttggcggt aatgcaaaga gcgatgcaat
    28141 ggaagcgatt gcggctgcta aaatcggtga ttttgagtta gcggatagga aaatcgctga
    28201 tgcagaagaa tcacttatac aagcgcatca tgcacaaaca gacatgttga cacaagaagc
    28261 acaaggaaat catatgcaag tgacattatt gactgttcac agtcaagatc acttaatgac
    28321 atctattgcg tttactgatt tagcaaaaga aatcatcgaa ctttatcgtc gcgtatacag
    28381 cgaataaata caacttcttt tttgaacgga agaagaaaaa tttttgaacg gattttttat
    28441 tttgttataa ggagaactct ctttatataa aaaacttata ggaggaagta aaaaatgaac
    28501 ggactatcaa gttggatgga gaaatacatc ctgccggttg ccgcaaaaat cggagcgcaa
    28561 aaacatttag ttgctttgcg tgatgctttt atcggcacac tgcccgcaac aatggcggga
    28621 tcagtcgctg taatgatcaa tgcaattatt cgtgatttac cgcaacagtt taacagtacc
    28681 tatgctgaca gtttagcagc agacaaagga atctttgccg tattgaatgt catcatcgga
    28741 atcaatggtt ttgtctggaa cggtacacta gcaatcgccg gattgatctt tgccttttct
    28801 tgggggtata atcttgcccg agcttatgga gtaaatgatc tagctggggg gatcgttggt
    28861 ttagctacct tgattcaagg tatttccttt ggtccagcat tatcagcagc attagatgtg
    28921 aatgttcctt caaatgtcgc agatgcaatc aatagttctg agattggtgc aacgattgca
    28981 gatggcacac tgtcagttgg attatggggt tggctaaaac tagatcattt gaatggaaac
    29041 gcttatttta cagttatgat catgggagct atttcagtca ttattttttg taaattgatg
    29101 cttgccaaca ttacgatcaa aatgcctgat tcagtaccac cagcagtatc taaagcattt
    29161 gcagccattt taccagcgac gatcgcttta tacgtcattg ctattatcaa ttatatcgta
    29221 ggtcgtgtag ctgatgggca actgatcatt gatttggtgc aaaaatatat tgcagaacca
    29281 ttcctaggat tatctcaagg gttgggagca gtattgatcg tcacagtatt tgttcaagta
    29341 ttctggttct ttggtattca tggaccaaat gtattggcac cagtgctaga gggaatctgg
    29401 ggacaagcac aactaatcaa tattgatatt ttccaaaaag gttataacgg aaaaactggt
    29461 acaccagccg tgttagatgc aatcgaagat ggaaaagctt atatgtgggt ccgcggatcg
    29521 ttcgatgctt ttgcatggtt tggcggatca ggcggaacga tcgtcttact gatagctatc
    29581 ttgattttct ctaaacgtgc agattatcta acagtagcta aattatccat tgggcctggt
    29641 atcttcaata tcaacgaacc agtgatgttt ggtctgccaa tcgtattgaa tgcaatcatg
    29701 tttatcccat tcttgattgc tccagtagta gcaacagcaa ttggatggac agctacttat
    29761 cttggcctag tcgctccggt atcgcagcaa gtcacatggg tagtaccgcc gttcttattg
    29821 tcattcttag caacaggagc ggattggcgg gcaccgatcg taacattagt atgtatggtc
    29881 gtgactttct tgatttgggc accatttgtc atctctgcaa acaaaatgga ccctgccgca
    29941 ggcgaacaac aataagatga acgtgaggag cgggacgctt taagtttcgt tcctcataat
    30001 gaattttcta ggaggaactg aaatgattac aattgccagt gcaatgcaac aagatgaaat
    30061 caaagaattg ttagaaggat acaatgaaga aggaatgact tttacattta aagaaaaaca
    30121 aggaatcaag ctgttctttg aaacaagtgc agaagatccg gaagttgcgg ctaaaaaagc
    30181 aaaggaacta atcaaagcac agccttgggg aacagtcctt tattttcaat caacagctgt
    30241 ttagaaaatg gtttaaaaag ataggatggt tgagatgata aaaaacggtt tatttttatg
    30301 tagtattggt tctttgatcg tgttgtatag tttgaatcca cattcgcaaa ggtatgattt
    30361 gtatagtatg gtcacaggtc tttttctaac aggattagga atctatttct tcatgaaggg
    30421 gaaaaaaaga gaagaagcaa aggagaaaaa taactaatgg aaatcacgaa aaaattaaat
    30481 attccagcat cgttttttta cgataaagtt atggactctg tccttttcga tgtacgtaaa
    30541 gcgaccggca aatcagtaac tagaaaacag ctgaaaaatt tggagtacgt caaacaattc
    30601 tcgcaaaata accgggctcg cataaaaatc gaagaagtca ttgaaaacca gtcttataag
    30661 tttcgtacat cgacaacgaa gaatgaattc gtcgttcatt atcaaatcaa accattggac
    30721 gattcgacct gtgaggttca ttacacagaa aaaatggagt cttatggcat gcttcaaaag
    30781 ttgaatgata tgcttttagg aacaatcttg atgtatttta aacggagacg tttcaaaaaa
    30841 atgctagata tgatcgaagc aacgtattaa atcaatgaaa catggtacta tttagttatt
    30901 gaaaaatgaa gtaagctaga aggaattttt cataggaaga tagatctttc ctgcgaatcc
    30961 tcactagctt ttttgtagga aggggcagca aaatgacaaa caaagtaaga gagctaggtg
    31021 tatcttcgat tgaggaaatg tttgatttag ccacttatgc atttaatggg gcagatacac
    31081 ctgaaaggcg agaacgtttt cgtacacttg cggagaattc ttggaattac ggattttttg
    31141 atgaggagga tcggctaaca agtcaagtca tggcgacccc ttttccagtg aatttttacg
    31201 gacaagagta cctaatggcc ggcattggct atgttgcttc ttatccagaa gcaaggggac
    31261 aaggcgggat caatcagatc atggaaaaga tccttgaaga ttgccgtgat cgtaaagtaa
    31321 gcttatctta tctggcgcca ttctcctatc ccttctatcg gcgctatggc tatgaacaat
    31381 catttgataa aatcagttat caactaaaca gtcgtgatgt gccgtttatc aaaaaaaaat
    31441 ctggaaaaat aaagcgtatg gattttgaag atgcaaaagc tgcaatcaga agaatttatg
    31501 aacagatgcc ggaaaaccaa cgagctggtc tgaagcgcga agactggtgg tatacgtata
    31561 aattcaagca aaaaggcaat taccagtttg ctttgcattt tgatgataat gaggaagcaa
    31621 cgggatatat cgtttatcag cttgcaacgt cttcctttat tattgcggag tggggatttt
    31681 tggatcatga tgctttttgc agccttgttc gcttcgtaag ttctcacaat ggtgcttttg
    31741 aaacatttga atattcatgc ggctacggcg gtaatgatca aaattatcta ttagaaaatc
    31801 catttgcttc tgtaatgatc acaccttata tgatggcgag gatcgtagat atttctttgt
    31861 ttttggaaaa ttatccattt aaaaagagac aagctgcatt tgccctacaa atagaagaag
    31921 atcattatgc gaaatggaat gaaggaatcg tggaagtaac aatcgaaaat ggaaaaaatc
    31981 atctctcaaa agtagaacaa acaaaactcc caatcgttcg aggaagtatc caacatatca
    32041 cgcagctatt attagggtat cgtaaagtgg aagagttggt attccaagaa agaatacagg
    32101 tgacaaaaga atatgaagaa acgttaagct caatcttgcc acagcaacag ccaattctta
    32161 atgactattt ttgataaaaa acgggtgatg acaatatttt tgtcaccacc cttttgtttt
    32221 tcatttggca tttgaaggaa aatgttaaag taaatcctct tttttcgtaa atttgaattt
    32281 tttgttttct tttgttttag ggttttcttt tttgtggtct tggattgctt ctttcgtaat
    32341 cttttcccga ttttttttag atgaatctaa gcgttctgcg tatacttttt cttttggctt
    32401 atcattcatt ggaaaggacc tccctacatt atttcattct acctctagtt tatcatctgt
    32461 taaggactag tggaaacgtt ttgcctaact tataagctga tttttttagc agataagctt
    32521 gcaagtaaaa aacaaacaag ctacaattga tttattgaaa ttgttcctgt agctcagtag
    32581 gatagagcaa tcgcctccta agcgataggt cgctggttca attccagtca ggaacgtata
    32641 aataacccct gtatcctttg cggtacaggg gtttgttttg tatttgtgcc aaaaatgtgc
    32701 caaactagtt atcattttta tataaactat cgaacacagt attgattttt tctttatcct
    32761 gatcttcaag ctctcgaaca atatgaagat aagtagacat tgtagtttct aaactgctat
    32821 gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa atagatgcat
    32881 gtgtatgcct tagtccatgc aaagtaaatt gcttttcaat ccctaattct tttaattttt
    32941 tgcgcaaata tttgctgacg gcgtttgatg aaactaatcc attttttaag ttgaaaaaaa
    33001 caaaattatg agggtttttg atttcaaaat tctcgaataa ttctttttga ttgattttaa
    33061 acttttttaa gagatcaatc gtatgtttat caatggaaat tttcctattt gaagtttcgt
    33121 tttttgtttt tccccattca tttaatttat aattccatgt tttattaata gtgatgattt
    33181 gatcttcgaa atcgatatct tcccatgtta atcctagaag ttcagcaaat cgcattccag
    33241 tggcaccagc aactaacacg agcataggag aagagtactg cgcagaaagg ttttcttcta
    33301 cgagtttcat taaagattta aattgatcat aatctaaata tttatcttct tttggcttca
    33361 atgaatcttt tcctttgatt actgcttttc tagttggatc aaacggaatt aatccttctt
    33421 ctacggcatc tttcaacgac gctctaatat ggttattaaa cttaataacg gtagatttta
    33481 catggtcttt agcatactta tttaaaaagc gttgatatcc cactctatct aaatcagaaa
    33541 tcaataccgc tggcatatat ttctttatgt tcattaacgt atcttcgtat tttctataag
    33601 tgataggaga tactgttcct tctttataaa gttgcatcca atcctcaaag taatctgata
    33661 ataatagatt tttgcgatcc cctttaaggc ctttgttcaa gttatattct aattcgttgg
    33721 cggcatcttt tgcttctcct tttgtcttaa atcctgattt tcttatcttc gtgtattttc
    33781 cgtcatcttt tttgtatgaa atttcgtatt gccaactatt tccacgtttg actaatcttg
    33841 ccataattga atcagctctc tttctctgat acaataggaa ctataaagaa gcccattgtt
    33901 tgggttttat tattttttgc acatcctttt tgcttgccgg cgcaaggatg tgctatttgt
    33961 cgttttaatg gtataattgg atgtgtatta gatcatccaa tcagaggtgt ttattatgat
    34021 taaagtgatt tttaaaagaa taactgaact tccagagttt agattatttg ttgattattg
    34081 gtatgtctgg ttttttattg ttgcattgct gataggtcta gatgtcatat tagaaagaaa
    34141 acttaagtaa gagttgagtt aataaattga attcctaaat gagatatttt gtagcccata
    34201 ttttgtttgc cgacatcacc taatactatg gagctaatcg ttttttctaa cagaccaaaa
    34261 tcagataatc tatttaagct accatctatt agctcttgtg aatagccaga catgctgata
    34321 tttccagtga aattttcgtt tccacgactc attaagcatg ctaacaattg aatctcaatc
    34381 gatgttaatt gattcagtac aagaagataa tactcctcgt tatcccatga agcgctgtta
    34441 attcgaagta aacaattctt ataaagattt ttatacatat ctattttatt ttgtgccttt
    34501 gatttttcta cctcgtcatt tatcccttcg aatattccaa tgagttggtc cttgttctct
    34561 aagttgaaaa catcttctgg aatttgatcg cgaatgtttt ctaatgattc gtttaaattt
    34621 ttgtagaatt tctcgatgcg tttgaaacgc ttctcgtttt tagacccaaa ataaagtgac
    34681 tgcacaacgc caccaatata aggaattgct ccaataccgc cttcaactat taattcaaga
    34741 tattcttttt tggttagttt ttcattgcta ttatccatag ttacctccta ttttgtcatt
    34801 tcctttacaa gcattgtgac tcctgatggc actcttttgt ataggtttta ttattgtgca
    34861 caatctaact cttagtgggg cgtggttcat ttatttttta cttactttac tattaatcat
    34921 atcccattga gcataggatt tttctatgtt cgatcctctt gaactaattt ctcctgtaaa
    34981 agttacttta tcgccctttg taaaggtatc agcattgttt aagtccttcg caaaaattac
    35041 atatgaaact ttaggtgttg cagaaatatc attccaactt ttatcatcgt actctttgtc
    35101 tgctataatg gctattctag tagtcattga ttcgataaca gttccttcaa aagtatatgt
    35161 tttaccacta atatatttag taaaagctgt attttgctca gtagctggag taatagaata
    35221 ataactatct aagaaagcac cccagtcatg attatttgtc tctaaaaatt cttcaaattg
    35281 agttaggctt ttattgctgc tgttagatgt agtagtttga gtatcagatg atgagctttc
    35341 ctgtttgtta ttgtcatttg cctcgtttga tgaggaaagc gagaaaccta cggtagtaaa
    35401 aataatcccg ataataagga gaatcatagg tgttttctta tttgtttttt tgataaatct
    35461 tacaattaac atgattaaac ctataataaa taaaacaata ccagtaaaac caaatatacc
    35521 gtccattaat agatcctctt tctcttttga tattgaatat tataaaccaa gcaattcttt
    35581 tttcttaaaa tcgaattctt cttgattaat aatcccttca tctaacaact ctttatactt
    35641 tttaatttca tctgcacttg aaatttgtgt ttctttggaa ttattatcta atatagattg
    35701 ttgcttctct atatattttt ttaaattttc catttcattc caatattttt ttgtaaacat
    35761 aacggtgttt tcatctttcg tggccgctaa tatacctccg cgactttcgt ttccgcctag
    35821 caaggtaaat tgtatataac cattactcat acctggtttt tttaattgta cggctgatat
    35881 atttttaaag gggatagttt tttctccttt aataccttga ttaactaaat ttataaatcc
    35941 ttttctaata attgaaatag aatcatcagt tactttaatc aaggtttttc ctggagattt
    36001 tattaaaatc tctttttcca ttttttcttc ctcatttctg tgatatgatt ttttttgtag
    36061 aagctcatat atgaggaaag agcccgtgtt gcagcacggg cttttttaat gttttgctga
    36121 aatcggtttt tttaataatt cttgatgtat tcatacatat tgccttgcgt aagaatattt
    36181 tttcttaaaa tagcattggc agacagcata acaagtgtat ctgagcttat tctgaataga
    36241 ataatactcc ataaattttt cgagattaaa ctgagattca tcagtcagtt cgttctcaat
    36301 atagatattt aataaaatta gaatagctat tttatctgct tctgtttcaa attttgagtg
    36361 aaaagttgta gaagtatcgt acaaaactga aaattcaaaa tgagaggcgc tgaaatgtgc
    36421 aagttcatga gataaatgaa aggcttctgc agtttcaccg tatagatttt cattcaaaaa
    36481 aatgattctg ggttttggat agtagaaacc aggctctttc atctccatat aaactacttt
    36541 caaattgtat tcgctcaaca tttctttcaa tttcaaatac atacaaacca tcactccaac
    36601 tattcatttt cctctaaagc tttagcaatt gcaatcgctt tacgcattgt ctccttagat
    36661 atttcttttc catcaaaaga aaaaacagta tcgtcttctg ataaatccac atgtttaggg
    36721 gtttctcttt cttctctacc tagaaggtag tctacagaga catcgaaata gtcagcaatc
    36781 cgacttaatt catctgaatt aggagtatta tttttccact ttgctagata accgttagaa
    36841 tatccaaaat taatttctaa ctgccgtata gatatctttt tcctcttcgc caagtctttt
    36901 attatttcat aggtattcat cgataaaaca acctttctaa atgtttacaa aaaaagttta
    36961 gaaaaatacg cagaaataat ttgactaatt ctgagtaatg agctatacta tatcttgtaa
    37021 acagatttaa caactaaaaa gacaacaaaa aacactattg atatgtaaat gcagaccgcc
    37081 aagaaagctt taaaatcaat gtttttatgt cttatttaat tatgctctta gtatagagta
    37141 ttactcagat actgtcaatt gaatttagaa aaaagttgtt aaatttgttt acgaatataa
    37201 aagaaaggaa tgagaaatat gaacacaccg caaattttta atttcgaaca aaacgaagtt
    37261 cggacaattt tagtaaatga tgaaccatat tttgtaggca aagacgttgc gagtgttttg
    37321 ggttattcaa acactaaaga tgctttgtcg cggcatgtag atttggaaga taagatgggg
    37381 tcgcgaatca cgacctcagg tcaatcaaga gagatgacaa tcatcaacga atctggtttg
    37441 tacagtttaa tcttaaaatc aaaacttccc tctgccaaaa aatttaaacg ttgggtaaca
    37501 agtgaagtgt tgccagcaat tagaaaacat ggaggttatc taactccaga aaaagtagaa
    37561 gaagctttgc ttaatccaga tacaatcatt caattagcaa ctcaactaaa agaagaaaga
    37621 actggaagat taatcgcaga acaaaagatt gcagagtacg aacctaaaat ctcctattta
    37681 gatagcatat tatcttctac agattcagta acaattagtc agattgcagc agattatggg
    37741 atgtctccac aacagatgaa taaattactt cataaactag gtattcagaa aaaagtcggt
    37801 aaccaatggt tattgtgcaa aaaacacatg aaccaaggat acacaaaatc tcatacaact
    37861 gagatcccga aagccgatgg tggcactaaa attgtaatga ataccaaatg gacacagaaa
    37921 gggcgtctat ttatctacga attactaaaa aaagaaggat attaccctca aatggattta
    37981 gaggaaattg gttagaaagg agttttagta tgactgacat tgcagaaatc actcaacgag
    38041 atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt cttaacttac accatgcttg
    38101 ctgaaagatt tggaattagc aaaagctact tatctttaat tttaaacggt aaaaagactt
    38161 ctgcagaagc aaacagaatt atagattcga ttatcactat gtacgaattg taaagggagg
    38221 aacagctaat gcaatatcta gaagcaaaaa tcccaattcc agaaggctat gtaattatct
    38281 cccaagtgga ttatgaggag ttaaaaaaag ctgatgatac tggtagatgg atgacgttgc
    38341 cagaagtgct agaacggatt aacagaaaat atgattggtt tacttctaga gttttaaaga
    38401 acccaagata tagaaatatt atcgatatcg agaaaaacaa aaatggattt gtctattatc
    38461 cagttgaagg aagagacaca tatctatttt taagaagtaa aacacttgaa tttttagaaa
    38521 caaatttttc ggaaatctta aggaggcaag cggatgggaa aatttaatag agcgctagtg
    38581 ttcagcgcac cgctaatcat ctacgcttta ggactttggg gaagcaggca agcgttgata
    38641 ggaacgattg tttacatggt ttggattttt atggggcttg atgaagctga agctgagtac
    38701 agagcgaaaa agccaaccga gggggctgac taaaaatgaa tagaaaagag aaactagaat
    38761 gtatattact attactcagt ttaattcttt cactaatttc tctattggga agtttgtatt
    38821 tttgatatca acaattttgt ggatttgggt tctatttaga gtaaatatca tttttgtttg
    38881 gtgattaagg agtttattaa aattaatttt ccctacttca aatgcaaggt aacaagatat
    38941 agcactttta ggaggaatat tta
//
LOCUS       DACENJ010000003        34859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.003, whole genome shotgun sequence.
ACCESSION   DACENJ010000003 DACENJ010000000
VERSION     DACENJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.003"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..556)
                     /locus_tag="IW748_000090"
     CDS             complement(<1..556)
                     /locus_tag="IW748_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103028.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQK"
     gene            complement(851..1186)
                     /locus_tag="IW748_000091"
     CDS             complement(851..1186)
                     /locus_tag="IW748_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1103029.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1173..1457)
                     /locus_tag="IW748_000092"
     CDS             complement(1173..1457)
                     /locus_tag="IW748_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1103030.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1513..3036)
                     /locus_tag="IW748_000093"
     CDS             complement(1513..3036)
                     /locus_tag="IW748_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1103031.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3029..4204)
                     /locus_tag="IW748_000094"
     CDS             complement(3029..4204)
                     /locus_tag="IW748_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1103032.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4208..4393)
                     /locus_tag="IW748_000095"
     CDS             complement(4208..4393)
                     /locus_tag="IW748_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103033.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4359..6053)
                     /locus_tag="IW748_000096"
     CDS             complement(4359..6053)
                     /locus_tag="IW748_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1103034.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6050..6523)
                     /locus_tag="IW748_000097"
     CDS             complement(6050..6523)
                     /locus_tag="IW748_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ1103035.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6592..6747)
                     /locus_tag="IW748_000098"
     CDS             complement(6592..6747)
                     /locus_tag="IW748_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ1103036.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(6881..7261)
                     /locus_tag="IW748_000099"
     CDS             complement(6881..7261)
                     /locus_tag="IW748_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1103037.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7265..7480)
                     /locus_tag="IW748_000100"
     CDS             complement(7265..7480)
                     /locus_tag="IW748_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103038.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7483..7890)
                     /locus_tag="IW748_000101"
     CDS             complement(7483..7890)
                     /locus_tag="IW748_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ1103039.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8156..9631)
                     /locus_tag="IW748_000102"
     CDS             complement(8156..9631)
                     /locus_tag="IW748_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ1103040.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9621..10469)
                     /locus_tag="IW748_000103"
     CDS             complement(9621..10469)
                     /locus_tag="IW748_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ1103041.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10506..10868)
                     /locus_tag="IW748_000104"
     CDS             complement(10506..10868)
                     /locus_tag="IW748_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103042.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11168..11792)
                     /locus_tag="IW748_000105"
                     /pseudo
     CDS             complement(11168..11792)
                     /locus_tag="IW748_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(11789..12100)
                     /locus_tag="IW748_000106"
     CDS             complement(11789..12100)
                     /locus_tag="IW748_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103043.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12144..12437)
                     /locus_tag="IW748_000107"
     CDS             complement(12144..12437)
                     /locus_tag="IW748_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103044.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12631..13278
                     /locus_tag="IW748_000108"
     CDS             12631..13278
                     /locus_tag="IW748_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1103045.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13339..14484
                     /locus_tag="IW748_000109"
     CDS             13339..14484
                     /locus_tag="IW748_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1103046.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14565..14870)
                     /locus_tag="IW748_000110"
     CDS             complement(14565..14870)
                     /locus_tag="IW748_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1103047.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(14895..15326)
                     /gene="ruvX"
                     /locus_tag="IW748_000111"
     CDS             complement(14895..15326)
                     /gene="ruvX"
                     /locus_tag="IW748_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1103048.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15323..15589)
                     /locus_tag="IW748_000112"
     CDS             complement(15323..15589)
                     /locus_tag="IW748_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1103049.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(15844..16569)
                     /locus_tag="IW748_000113"
     CDS             complement(15844..16569)
                     /locus_tag="IW748_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1103050.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(16930..17000)
                     /locus_tag="IW748_000114"
     tRNA            complement(16930..17000)
                     /locus_tag="IW748_000114"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16966..16968),aa:Gly,seq:ccc)
     gene            17117..17869
                     /locus_tag="IW748_000115"
     CDS             17117..17869
                     /locus_tag="IW748_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1103051.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17885..18364
                     /locus_tag="IW748_000116"
     CDS             17885..18364
                     /locus_tag="IW748_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1103052.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18615..20195)
                     /locus_tag="IW748_000117"
     CDS             complement(18615..20195)
                     /locus_tag="IW748_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1103053.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20188..20931)
                     /locus_tag="IW748_000118"
     CDS             complement(20188..20931)
                     /locus_tag="IW748_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103054.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21107..21883)
                     /locus_tag="IW748_000119"
     CDS             complement(21107..21883)
                     /locus_tag="IW748_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1103055.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22131..22967)
                     /locus_tag="IW748_000120"
     CDS             complement(22131..22967)
                     /locus_tag="IW748_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1103056.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(22978..23907)
                     /locus_tag="IW748_000121"
     CDS             complement(22978..23907)
                     /locus_tag="IW748_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1103057.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24099..24452
                     /locus_tag="IW748_000122"
     CDS             24099..24452
                     /locus_tag="IW748_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1103058.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24526..25182)
                     /locus_tag="IW748_000123"
     CDS             complement(24526..25182)
                     /locus_tag="IW748_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1103059.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25408..27342
                     /locus_tag="IW748_000124"
     CDS             25408..27342
                     /locus_tag="IW748_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1103060.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27428..28651)
                     /locus_tag="IW748_000125"
     CDS             complement(27428..28651)
                     /locus_tag="IW748_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1103061.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(28645..30543)
                     /locus_tag="IW748_000126"
     CDS             complement(28645..30543)
                     /locus_tag="IW748_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1103062.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30537..31655)
                     /locus_tag="IW748_000127"
     CDS             complement(30537..31655)
                     /locus_tag="IW748_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1103063.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(31738..32571)
                     /locus_tag="IW748_000128"
     CDS             complement(31738..32571)
                     /locus_tag="IW748_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1103064.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(32564..33481)
                     /locus_tag="IW748_000129"
     CDS             complement(32564..33481)
                     /locus_tag="IW748_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1103065.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(33504..33683)
                     /locus_tag="IW748_000130"
     CDS             complement(33504..33683)
                     /locus_tag="IW748_000130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103066.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(33708..34832)
                     /locus_tag="IW748_000131"
     CDS             complement(33708..34832)
                     /locus_tag="IW748_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1103067.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        10446 a         7050 c         5700 g        11663 t
ORIGIN      
        1 ctttttgcat cgacgaatgt tttctttccc aagataacgt aaatagattt gactctggac
       61 ttataaagtc tacttcacct tctagtatta ctttcttatg caaatattcc tctaataaat
      121 atagacttgc taaagcatac aacacatttt ccaaatttgc ttccttataa tttgaagaac
      181 gaccatgttt tactctgtta tatattttcc accaatctgg tgtatcttca ggccacccat
      241 ctccaaaagg ttttagtgaa gtcaatgtta aagaattcat aaccctaaca ccattatcca
      301 aacacattaa ttcgctagat ttaataacag ctttaaaatc agttatgctt ttattgctac
      361 tgctatcaaa tccgcaaagt tctttgaaaa taatctcaca ctccgaacca atcgctagta
      421 gtagtttttg atattcaata gagaatgttt tgaaattatt ctcatcgagc atcacaaaat
      481 ctgtggtatt aataaactca ttttctaaat gtagataata cttccaataa aaatttgtaa
      541 actctgcttt gtccattaaa tcacctcttt tgaattataa caaaaaaaac acgtccacaa
      601 cagacgtgtt ctttgatttc tacacaatca gcttgccaaa accatccaat cagtcctagc
      661 atgcctgtat taaaattgaa cgttcatcat ctatcatctt tcttagtcca gcatgtttgc
      721 aaccacacct acaattttca gtccaacaat agatttcgac tagctaagtc ttgcaaaatt
      781 caggcagcta acccttcatt tactacctta attatactaa atacccacaa cgtttacaag
      841 tgtaatcatt ctaagaaatc tgtttagcta tgatcgttgt ataatcttta aacgccgttc
      901 cttcattgat tgaaataatt ttataactct tgtttttcca agctactttc atatcgtttg
      961 ttagttcata atcttgatcg tatcgaataa taaacgtcaa cgttccttca agaatggtac
     1021 ctacactagc aagcacgtct tttagcattt gtgaacgcac attagcccaa cattcaaata
     1081 cagtttcttc actatccacc atctgcccat cttcatcttt tacccgtttt gtctttttaa
     1141 acttgactct ctcagtcaat tcatttacat tcttaatgat tggcataata agcacctctc
     1201 atttgttgta ctaatgcttg taccgttact ggtatccgtt cgctgctggc ttcttgacgg
     1261 ttcaaatacc aatgttgagt aagtaatgac accgccaaat caaattgctg ataattatcc
     1321 atatctttat ccgttgcgct actatcaata gcacttttta tatattcgcc tgctgtatca
     1381 atcaattgtt gaataaagtc atcatcaata gtgtgatcga tccgcatact tttttttatg
     1441 ctatccaaaa ttaccatttt atctctccta atgaaaaaaa ggcagtggga taaaccgccg
     1501 cccaatattt ttctatactt ttggtgttac aactggcgta aactcaatat acactgaagc
     1561 attttgatca attttcttat aatcgttgcg aacgatcact gcaagtcctt gtgagtagta
     1621 atcgaatttt tcccattgag tcgttacttg atttctacga gcaacaaaga tagattgtgc
     1681 aatatccccc ataatcatag ggaatgttcc agctttagga ttggcaaaca atgtatctgc
     1741 aatcaatact actggcatac cgaataatga tttaccacta ggtgctgtca catcgggttg
     1801 taaaatgtaa cgtccatctg aatctttcaa tgtatctagg tggtaatatc cgctttggtt
     1861 tagaattacc attttattta atgctgggtc taatgccaca ttgtatagtt gtttcaaatc
     1921 gtccaacgta gcagccgttt tcttagtgaa tgtttttaat aaatccatga tatgcttatt
     1981 gtcagtgttt tctaccaatt tcgctaactg atctttgacc tcttgtacaa tattcactgc
     2041 tgaatcttcc acaacctcat ttgataaggc aatcttacca gcacgagttt ctactttata
     2101 atcaactgaa gtaaacattt cagcgtcaat atcaccaatt tcagcaagtt cagcttttgt
     2161 tgctaacact gcttgttggt tagttgctac tggatattta ccttgtccat ttgatactgt
     2221 ttttactgtt gcatattgag ctaagttata atttgaacgt ttcaaatcaa agacttcacc
     2281 gattacttct tcgggaacaa ctgccgctgc atttacagta gtgactccat cacgcacttc
     2341 gccttgtgaa cggatatatt cttcataccc acgcaattct gttttttcgt tatcgattaa
     2401 agttttttcc atgttattac tctccttttg tgctttagaa gtggattcta agctattgtt
     2461 tgtttgtttt gcttggttgt tactcataaa cgattcgtaa gaacgcttat ttacttggac
     2521 atttgatgaa tcataagcgg gaacggtcac gacactaatt tcattaagtg ctttgatttt
     2581 atctattgat cgttcaattg tgccatcttc ttttttgtcg aatgaatcta atccaagcac
     2641 gaacccaaag gacatagaat caacaacccc ttttgagata ttctcataca catcatttga
     2701 ataggtcgtg tcggttagct ctgcttcaaa atgtaatcct gtatcatcga cattcaattt
     2761 caacgtacct gctttgacac tagctagtgg ctttgaataa tcgtgtccat ataataagaa
     2821 cacatttgat aaatccacct cttttaatgc ttcgggtgta atcacttcta caaagccgcc
     2881 taaatctttt gacggttgcc cgaatttcaa cgcatagcct gaaatagttt tgccgtctgt
     2941 tggttgtttt tcattttctt tctctacttt gggagaatcg gctgttagct cagcttcttc
     3001 tgtcagccgt ttttcctttt cttcttcatt attcaactgg tgtaactcct tcctgttctt
     3061 ttaattgata tttctccaac gtatctaaat acgtaaagtt caaacttgct agtaaacgat
     3121 cgccaccatc catggggggt agccccattt tcgaccgccc ctcattaatc gtgagaagtg
     3181 accctttgac ctgatccaat acatttttta cttttgtttc aggatcagtc tctagtaact
     3241 gatcggaatt aaagcggata ttggtttcaa gtttcgtatc taattcactc acaaacacat
     3301 taaagtaatg aatcagcgta ctttggatat actggagatt actttgaacg gtgctagaat
     3361 gttcattttc aacgcctaaa cgctcaatcg gtacaccaaa cgccttggca atttgttttg
     3421 tgttccaatc attggaattg ataagcttca aaacatcggt gtttatttct aatgttttgt
     3481 agtccattgt ttcatcaaga atgatcgttc taagagcatt atctccacta cttgaaccgt
     3541 tggcttcttc aaacttttca cgaattgcgt tctttgctga tccgtctaaa tctgacttat
     3601 gaaccttcaa aatacctgac ccactgacac cacgagtaaa gaaattgtgc aatgtgcggt
     3661 tgccagcttg ttgaattttc agttcatcac gtaacgcata aagtggtggt aatcctgtta
     3721 aaccatcttg ggtgaaatat ttgaagtgca aaatatcgct agacttcaca cgtctttttt
     3781 tatcaccaat ctgataagac aacgtaccat tatctaattg agtaaccgtt acacttgagt
     3841 taggtaataa gtgaatttct tctacctttt caccgttgcg tttaatctct gcaaagctat
     3901 taccgtttag caacatattg accgccaaag caaactttaa atgccaacca tccatttctg
     3961 aattaggttt ctcgtttagt aacttcacca gttcattatc agcttgtggc atattgtttt
     4021 tgaccaattg aatcggactt gaagcaatat cactagcaat aattcgaacg gctgcaaaca
     4081 catcactatt tcttaatgca cgaacgcttg taaagctggt ggtgtaatca tccgattgga
     4141 ttgataccac atgttgtagg aaaggatctc ccgttgtatt ttgtctcgct ttaaaaaatg
     4201 ccatctttta acctcccttc tgttctgtat ttagcatgac cgctacccca ataagcacta
     4261 tgcccagcgc taacaagcca tagtagacat ttgttagtaa ggtgatagca accaacacac
     4321 acaccaatcc aagaagtaaa agaacggtat gaatgtaatt agaaagaaaa ttcttcactt
     4381 gtataaaatt cattgtctgc tttcgctcct tccgttgtgg tgaagtaatc cattgcaaac
     4441 acataggcat tgattaatgc agctattgga tcaattttgt tactgttctt tgatttatta
     4501 atctgaatgc cgttgttatc cgtttttaaa atagcgttgt tcactgcatg ggtgagaatc
     4561 gtatttttat tgtgaataac gttgccttca taaacttgtt ctctaaaagt tcgggtcgga
     4621 acgtttagag taatcgttcc ttgtcttact tctaacattg ggtagttggc tttttcagct
     4681 ttactgataa gcgaattagc gttatacggg tcataacaaa tccccataca ttccaaatca
     4741 ttttcttgaa tcaaatcttg aataaactga aatacttggt catagtccac gattccactt
     4801 tctaattgag taatggaaca ctcaccagct ttttctagtt ctcgataagg caaaccgtcg
     4861 cgcttttctt tgtcttgaag cccatatttg gtggctacaa agctatgtga atcacaataa
     4921 agttcgccgt tatctagcgg tacgatccat gaaacgctag ttaaatcatc tgttttagat
     4981 aaatccactc cgatataaac gggcttgcct gtaatatctt gtggttctac ttcggttgcc
     5041 tgccagtctt cagcaatcat atagctgtcc tcactcgctt gtctccataa attgaagttt
     5101 ttcaccaata cagaattaag gttattttgt ttcaaagcaa ggttcacatc atcttgaatg
     5161 gttggaatca ttactttttt gatttcttca ctttcaaaaa tcggattggc tttgatccat
     5221 ttctcttgat cgtgaatttc ttcgggatcg tccaattccc atatcgctat aaagtacctg
     5281 tcggcttgtt cttcgccttt tagcacacga tctaacaata agtattcctc gtacatagga
     5341 acgtttaaat caagcccagc ggtactaata acacacaata agccgttctt ttgttgggtc
     5401 attcctgact taatgacgtt gtacgtttta cgagtttttg cttcgtgcca ctcgtcaaga
     5461 attgcggttg ttggtgcaaa cccatctagc gtgctggttt cactagccag tgccatagcg
     5521 aacgaattag agggcaagtg ctgaatctta gagttcataa ttttcagttg tggtcttaaa
     5581 aacttactag acttgaccac gtttctaagt gaattagaca gcatatcata gcccaattta
     5641 gcttgtttca aagcgttact tacaaacaat acttgccttg cttctgctgg ttcttgttcc
     5701 ataatcaacg cattggcagc catgccactt gcgatatacg ttttcccatt cttacggctc
     5761 atactggtaa acgctcgatt gaatcgcctg taattccctt ctttagtacg ccagccataa
     5821 agactgccta gaatgaattt ttgaaatcct aacatcttga ttgctttacc gtccgtagat
     5881 ggtagcaact caatgaattt aatcgctttg gtggcttgtt cttcatcgaa cacataaggg
     5941 aagctatctg acttagaacg ctccaaatcg cttatatgac gctcacacgc ttggataatt
     6001 ttcttgggtg caatcagctt acctgatagg actttctcaa tgtaatcatt catgaattca
     6061 tcaactcttt gaacgggtct tcgggttcat catttttaac cttgttcaaa gctagttttg
     6121 atcgtgattc taaggtcata cctagtgagg tggcttgttt tttcaaatca ctcatagctt
     6181 gtgattgaac ccgaaccgct ggattggctt tctttactcc gttttcgcat gtgataaacg
     6241 tacctgattt tcttatctcg gcttcggctg ttttcattcg agcaaaggct aaacaatagg
     6301 ctaccaatga accataatct gcttcactca atggataatc tttttttaat gttggtacga
     6361 tacgattcca ttcacttcta gcgctcgctg ccatccagtc agggggcgtg atatttatta
     6421 atggttgctg cttgaacaat tcttctctag cgtccacacg gattgctttt tcttcacttg
     6481 atatattgcc ttttgtatcg tgtaatattt tagggtgtcc catgttgtcc tcctttcaga
     6541 caaaagaaaa aggaacgtaa gcaattaagc tcacattcct tcgtttttcg atcagaattt
     6601 ttctttatct aatttatcta tataaatgat ttttccgttc tgcatttgaa tggtcacttg
     6661 accaaaatga ggtaactcaa tggtgcgctg tttccctgtt ttatcagtaa taacaattgt
     6721 atttttcaat aaatctccta ctttcattat atactttttc acttgtgatt acaagtgtaa
     6781 tcgttatttg taattaccct tatgcctttt cacaacggtt atttgtacca aaatgagtag
     6841 gcatcgattg tcagtaagtt tcacactatg cccccttaac tcaaagggta ctcttttctc
     6901 gtctttgtct ttcattggct gtcttggaat tatggtgtgc atggcataaa gaacgaaaat
     6961 tagtttcatc taaccgtcta ttccaatcat cttttagttc aattttgtga tctacaacat
     7021 cagctttcct tatcaagcct tcttctaggc aatcctcaca tactggatga tttaaccgat
     7081 acaagtatga agcctttcgc catgttctac tttgatagaa ttgaaaatat ttaccttcgc
     7141 tttgctttct ttcttcacgc ttaattgccg tttgctttgc tttatgcttc ccacaatact
     7201 tctgcctata atcaatcaat gttttacatc cagcatgatt acattgtttt ttaactgcca
     7261 tagattacca accaaagcga tctcgttcgt cagaatcatc ttgttcccat tttgatttgt
     7321 attcaatata aggcgaaact atcttcaagc acgttccgtc tttaaaatta actttagtga
     7381 ttttatcttc ctcaaaacta ataaagtcta catcttcttg agtgtttgaa tactcaatgt
     7441 gtttctcacc atcgaagtat ctaatacttt ccatatgcat aattattcta cctccaataa
     7501 attagttatc aattcatcat ctgttagtag caaagccaag aacttatcat ggatgtttaa
     7561 aaacaaatta tcctcattcg atgtaagctc tcgttgtctc gctcgatcat ataggttaag
     7621 cagacaatta aattcatcca ttagctcacc tgtatcatca tagttgccac tatctaccaa
     7681 tgatcccact actagcgatt gacaccacgc tactgactcc tgcagccaat caggttcttc
     7741 ttccattatc tttaatactt ctggatcaaa tgtggaataa tcttctgtta ccaatcccac
     7801 actaaatgcc agcgtgttca ttagaaaccg atccatttca tctgtatatt gaaagttcgg
     7861 tgctttgttt gttgaaataa tattgagcat ttactttcct ccttgattcg taacacgtgg
     7921 taacacctac gtaacaggta cccgttacca ctcaatccca acggggacaa gggttctagc
     7981 gttttggtaa caggtaacgg gtaaaatcca gtaacattat tccccagcat attagctaac
     8041 tatttcaaaa gttttaagga aataaggtgt taccccgtta ccaaattgct gtatcccttg
     8101 gggcagtaag ggtaaactag taacgggcta ggtgttactg acccgttact acccgttact
     8161 ttttgtactt tccagctcga tttttatttt cataggcatt tgacttattg taaaatcctc
     8221 gtcgtttatt tctttttggc agttgcttcc gttcccaacc aggaatactg ctaataatca
     8281 aggcaatctt tttagtttct gcatctgatc gaccattcaa taaatccttt gcttgtttat
     8341 caaaaacagc ttccaaaata tcagcggtga gtacgctatc aactaaataa aactcttgtt
     8401 gcatagtagc tccattatag aaatgctgta aatttttctc attacattta ctgtcaggat
     8461 agttctgata ataagaacgt ctcgcccacg ctggagcttc ttcccattta tacgggattt
     8521 ccatttccaa atatttagtg attgcttctt taattggatt ctccatcttg gcatcctctt
     8581 gatagtcttt tgcaatttct aagacttctt tatcttcatc gggactaaaa tagattcgtt
     8641 gtcctttatc aaaaagcacc tttgcttcag ctaacacttg aagaaaatat tcatcctttg
     8701 ttttaaacgg attcatcttt ggcttattct tacaaggcaa aggataaaaa cggcggtttc
     8761 ctgtatcatc tttcaaatac tgcccatcat tcgacgttcc aataaataca cattgccttg
     8821 cccaagctat gacattccga ccataaggag gtctaatttt atcgatcttg gcactgatga
     8881 agttttttat ctgttcaatt ttagttttgt tcattgaaga taattctcca agttcaataa
     8941 ttacattgcc ttgaagctgc atataatcat ctttcttttc acctagcgaa tctaatgaat
     9001 cggtgaaaaa atcatccgtg taaagtaatg ctggaacggt actcttacca atcccttgcg
     9061 gtccatccaa aactggaacg atctcaaact ttattcctgg tttatagata cgtgcgactg
     9121 atccgactat ccaacgtttt gttacttctc gtgtgtaaag gttgtcgtct acccctaaaa
     9181 ggtcaataaa cagtgtttcc agtctaggct gttgatccca tttaactttt tcgatacgtt
     9241 cttttactgg gttgaaaaca ttctttctag ccacatttac tactgctttg tgtattttgt
     9301 tgtcatcaaa aactacattg tagttttctt caatatatgc cgttaaggca tcctcaaaac
     9361 tgtcttccca ttctccagcg gtacgattga tccaaggaga atcttttaat aggtgaatgt
     9421 atccgctaaa ttcgttataa gcaattgttc cagctaaatt gctatcattg gttaaaatca
     9481 attctgcatt cacaacactg ttacttttaa tctgatcgtc tttattgcat cttaatcgtg
     9541 acatccaggt tttcaattca atgatattgt cttcacgctc gctggcttcg ggaaagtcta
     9601 aaatactatc taatttatgc ttatgagcca tgagctttcc ccctcaaatc ttttttaatc
     9661 atcgatcgaa taatttgatt cacttcttta tcttttagcg gtggttcatt gtacgcattt
     9721 gctgtataaa tcaattctgc taaagtttga ttatctacac cgtgccatag taaccaaccg
     9781 cataacttag ttaaatgatc gtttctacta ccttcgccag cgccttgaac aatcatatct
     9841 aatttttttg ctgtccctgt ttttgctacg ggtcggtatt gagtctttat ttcttgcggt
     9901 ttttttagca gtaggttcac tagccaatcg ggagctggtg caatattatt tagagatacg
     9961 ttttcaatag gttcatatgc tctcccaaca cttcttgaaa taatcatagt tggcgctatt
    10021 ggaactcctt tagtgataat atcaatcccg cttaaacttt tacctttatc atcacgcccg
    10081 aagatatcag caaatgcacc tgtttcttgt tggataatta caccatcagg aattttaaag
    10141 aagaaatgga ttcctttacc tggtgtgatt tccgtataag tagaaggaaa tggatcatac
    10201 attgaagcaa gttttttata attctcaacg ccgtttcgtg tttcagaatg ttgatctagg
    10261 tcaactacca ccatttgatg atcttcaagc attaacccaa tatttctagt tgcatcttcc
    10321 gtccaccatt cattaatttg attagaatcg ttagttgcat cattccaacc actagagcct
    10381 tttattggaa ttttagttcc aggagataag ggataaattg accatcccga cttttgtaat
    10441 tccgttgctg cttgataatt atttgccaat aaacctctcc tcctttttca aaatactggt
    10501 ataaatcatt cagacacctc catgagcttc aactgctcaa gttcgtattt caacccatcc
    10561 cgaacaaatt cagcaccttt taattgattc tgatacttta cccatgtatt aaatgttttc
    10621 acccttgctt cttgcatatc agtaatgttt aaccatgcgg ttaaaatctg attttttagc
    10681 tcgttgattt ctcgacaaat tattttgtgt tctgcatgtt ttttgagtac atacaaaact
    10741 gttagttttt cattgaatga atttccaaag taggttttcc cgatattatt ttctagttca
    10801 gcgatccctt gattaatttc ctctaattca aggctatcga gttggtttaa tagttttgtt
    10861 ttgtacatct acttatgccc tccagttctt aattgatagt cgtactcgtc ataagcagtg
    10921 aagagatata acgctaatcc tgcaaagata gctatttttg caattaatgg taataagccg
    10981 aaaattccgc cgattaaaat gcttcctata attaagtttc gtttcataat ggttccctcc
    11041 gattttttaa tatggtataa tcggaacaaa gaatatcggg ttttatccca attaagttgc
    11101 ctttatcctg tccgccaaga ctgttatgga taaggctttt tctttgttct cgatcatgtt
    11161 aatgctccta agttgtctgc aattcagcag aacgattata aattttctca ctcactgaaa
    11221 actgcaaccc atatttttct ttaacagtca ttaattctac tgtctcatct aaaataggtt
    11281 cacgatcaac caacatttca ggagtcatat ctttcttttt taccattttg gaatgaccat
    11341 aaatagttga tacagcttta ttggctatca cattcgcttt aatgtagtct actggttttg
    11401 gttcagtaat agcgtggctt aatttagtca ttgcttcttt ttggtgttct ttgtctagca
    11461 tgcggaatac ttgaaagcct tcaagtcctg tggactggcg tagttctttg atgatgtcga
    11521 aaatccagtc ttgaaatgcc tcggcttctt ttttacggct tttgaatgcc atgcggtaaa
    11581 tatttttttt cgttgatgat gtttacatct tgtgttctgc ccaatgaatc gatgacctta
    11641 ctagtagtaa ccccatcttt aggcaaagag tgaatggctc tagtaacctg tttaagacct
    11701 aacgcttccg caacatcttt cgccactgcc caccattcat cattaatatc gacaaatctt
    11761 ataatgtgtc cgttccaaat ttcagttttc attcaacgtc ctccagttca agccctagca
    11821 agctcgctaa caactcaact tgctgataca aaatttcttg atactcttca atagaaagtc
    11881 gttctttaaa ctctgcttgt actaaatcgt tcaagccacg caatactccg cttaccgttg
    11941 gttcttgtgg tagcaatttt tgatagtcca tttgttcgta caaacattca aacgtttctt
    12001 ctagcgcttt gccttccaag tttgctaatt ctgccgctgt gtcttctgta agaattgttg
    12061 ataatacttt ttcgattgct ttattttgtg tcgattccat tattatttcc tcctattaat
    12121 ccctttgccc ccacggcttg cgtttatttt tggtattctt ccataaagcg atcacattca
    12181 tcttgtgaaa ttcttacgac tccatcaact ataatcaact ttaaaccgtt aggaacatat
    12241 ttctgcatca tagtattgta tgaaacgttc atatacttag cagcttgtgc cttgttcata
    12301 tattttggaa actggtactt tttttctaat aatgaaagta acgttccttg agtttgctct
    12361 acttgttgaa caactcgatc gtcaataatt ttaaaagctg gatcatcaag cgtgagtttg
    12421 tcaattttta attccatatt tatcccccct cgttagcata catttctata caaagatttt
    12481 aaaaaaatgc aagaggctct tgaatacatt tctatacctc ttataaactc aatataccat
    12541 catacctttt catttgcaag aattttaaca tacttttttg aacttttatg tttcacacat
    12601 atttaaatcg tgatatagta gagaaaatat atgaatggtg gtgaaaatgt ggctagaaaa
    12661 aaataccccg aagcagtgga gacaggacaa agaattagac tggctagaat tgaaaaggga
    12721 ctctcaatgg aacaacttgg aggtaaatta tccccaccgg ccagtaaagg tgccgttagt
    12781 aattgggaaa atggctataa tcttcccaat aacaccaggc ttaaacaact ttcaaaggta
    12841 ttagatgtat ccactaccta tctattaaac ggatcttata cattaagaga tatgcatatg
    12901 atgcccgaaa ttaagcgaaa tgtgcttgaa aagcaattga aaaaaactat tgaaaaaaat
    12961 aactctataa ttgattctaa tctttctaat gctatcaata acatcgaaga agataacctg
    13021 actgtctcac agaagattta tttaacaaac gcaataaaat atcttaaaac aggatctcga
    13081 attgaatttc ctgacgggag taccccaatt gcttatctcg cagctcttct aaatacgctt
    13141 ttacgatatg actcattaat taatgattca gaaaaaaata cagatgagaa aaaagagtat
    13201 caagaatttt atgaaaatga tcttctaaaa gagttcacag ctcttgttac tgagctaaat
    13261 aaacactatt caaaataata ataaactgcc tcctccaacc gcccccacgg cttgctacac
    13321 tatggaggga aaactattat ggcaacattc aagcaatatg aaactaaaaa aggtaaattt
    13381 tggctctatg aagcctatct tggaatgaac aaaatgactg gcaaacctga taaggttcgt
    13441 agacgaggtt acaaaacaaa aaaagaagcc cagcttgcct tgtcacgctt gcaagtggac
    13501 tacgataaaa acggtattaa aaaagcgaac aacgaaacgt ttaaagaagt ttatgacttg
    13561 tggctagaaa catataaaac cactgtccga gaagccacct tcatgaaaac tgaaatcaag
    13621 tttagaaagt ggattcttcc aaaatatgga aatcttcgtg tgaatgaagt cacggtaaaa
    13681 aaagcccaac aaattgtaaa tagctgggct gaaactactg accaatacaa ggtacttcat
    13741 tcaacttcta agcgtatttt tagctacgct atcaatcttg ggattattga gactaatcca
    13801 ctagacaacg tattaatgcc taaaagaacc accactaagc ataaagaaaa ggtcaaagtt
    13861 tacagtaaag aacaactgat caccttgttt gattacctaa acagcaaacc tgaaacttat
    13921 cgaaacgact ttgataaaac gttactacgc tttctctttt atagcggggt taggatcagt
    13981 gaagcccttg cattgaattg gtcagacatt gattttcaag aaaatacggt caccatcaat
    14041 aaaacactaa gccaatccaa aaatggctat aaaatcagtg atcctaaaac tgattgtagt
    14101 gctggggttc ttccactaga tgaaagaaca atttctatat taaaaaaatg gcagattaat
    14161 cagcggaaat atatgctgac acttggtgta actgatccga ctatgatttt ttgtggtatc
    14221 tataaagaac taatcacaca ccacgctatt tatgcaagat tgattactat ttgtgaaaaa
    14281 gctatggttc catttctcgg tgtgcatgta gcaagacaca ctcatgcttc aatgctcttg
    14341 gacgctggag ctactatggt agaagttcaa actagattac ggcatacaaa aattagtcag
    14401 acgatcgatt gttacggtca cttggcaaaa gaaacgaaag aaaagactgt tgaaaaatta
    14461 gtacaacact taaatattga ctagatggca tacaatatgg catacagtaa aaaataaaaa
    14521 aaggagaaac cctatcatat caaggtttct cctagcttta tagtctattc ttcttcttcc
    14581 gccatgaatg tattgaatac ttcttcaatc atgtcccatt ctgcgtctgt ttcgatttgt
    14641 tctaactcgc cttctgttcc atcttcgttt tcgatgtatg cataagcttg taattctaca
    14701 tcttcatctt ctgaaacacc agcaggatat aaaagaacat agttacgtcc aaattcttct
    14761 tgtccgtcaa ctgttagtag gatttcatat aatgtttcat ttccttggtc atccaccaat
    14821 gtgatgtgtt cgtgtccttc atggtcgtgg gaatggttat gttctgtcat aagattacct
    14881 cttttctttt attattaaaa taattgctta cctgagccgt ctaaataatt ttgtaagatc
    14941 atcacagctg ctaatttatc gatgactttt tttcgtttgg ctcttgaagt atttgcctgc
    15001 tctactagca tacgttctgc ctgaacagtg gttaatcgtt catcttgata atcaactggc
    15061 aaggagaata gttctttgat cttttctccg tatgacatgg aagcttctgc ccttggtccg
    15121 atggaattgt tcatattttt aggtaatcca actacaaacc ggtctacttc atattgtgct
    15181 actaattcct tcaaccgatc aaagccaaac tcttcttctg cttcattgat ccgaatgatc
    15241 tctactcctt gagctgtcca gccaaagggg tcactgatag caacacctac tgtcttagaa
    15301 ccgacatcca agcccattac tctcataaat tcactccatg atttgagagg tagtccttga
    15361 caattacttc catgatctca tcgcgttcgt gacggcgaat caaatttcga gcatcacgat
    15421 aacgaggaat gtaggccggg tctccagaaa gcaagtagcc gacaatctga ttgattggat
    15481 tgtagccttt ttcttccaaa gcccgataaa caatcgctaa tgtttcgctc acttcttttt
    15541 tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt aaaacccatg attctacacc
    15601 tctttcccgc aaggtaattc cagcttacct tctacaacct tattttactc gaagaaaggg
    15661 aaaactacaa acaaatattc caaacttccg aataaattaa gaaacactat ttgtatgttt
    15721 ttttcctttt ttctgtagct ttatcaaaag tactcttttc ataaagtata cgctatattt
    15781 ctttgaatgt atgtttttac acaatttttt cataatattc ataaaaaatt cgaatagctg
    15841 ctactaattt tttgtttttg ttgcttgtcc tgtctcataa ttgatcgtgt agccatcttg
    15901 aacattgtag ataaagacat tgaattcaat ttttttgtct tcaatgcttt ctgcttccaa
    15961 ttgcactccc ctggctacca gttcattccc ctcaaaatga ggagtgacac ggtagcgtac
    16021 atgatgattt gtttctttga tatatgccgc aatatcattt tcgtgagcaa gcatgtatgg
    16081 agcattcaac gaacgtgtcc ctgtcatcaa gtttttaatg ttgttatttt gaccagtcag
    16141 ctggaaacca attaaatggc tgcgattata taaccattgc ccgtcactta atttcttttg
    16201 tttccaacct gtcgggtcaa tatacaaagg ctctctcttt tcagtaggga acaaatcttt
    16261 tccaagcata gcattggctt gtcctactct gttcaattta tcaatatttg aatagctttc
    16321 ccatgttcct ttggaaaggc taagatcctc ttttgtaaat tccggctgat tcccattgat
    16381 ttctatttct tgatgttttc catcatattc tggcacatcc gctacttgag aagcttgtgg
    16441 tgtggaatca aaaatagttg taatctgctg aaagatatct gaatcttttt gtgtggtgga
    16501 aacaccgaat gcccctgcaa tcacagcagc gacgatcccc acgatcgcct tggttgtttt
    16561 tttgctcatt tctttactcc tttactaaca gctccctaaa tcttaacata tgttccccta
    16621 taaataaata ctagaaagaa atagaaacag caaaaaataa aacctgtttt aagatggctt
    16681 aaaaatacaa aaaagagacg gattcctcgt ctctaatcag tcaccactct atctgtaagg
    16741 tatttattat tattcaaaaa ttttcttcat actcaagaca atacactgat ttcacaaccg
    16801 cttggtaaac ggtgcgcaaa agcttgcatc tgcggtaata agtccatcca agttaaaaac
    16861 ataagaagtt gtttttgctt ggctgttctt attacttgtt gcagagcagc tttttcacaa
    16921 cctattttaa gcgggtgacg ggaatcgaac ccgcgacatc agcttgggaa gctgggattt
    16981 taccactaaa ctacacccgc aatacaaaaa cagtatacgc ctcttttatt ccttcctcaa
    17041 gtttttttat tctttacgct tctggtatgt tttgtagtaa aataaaacta atcaatcaat
    17101 tattggagaa gatgctatga cgggaaaaga attagatctg ttgctggaca cttgtttact
    17161 cgctgggaaa atcatgatgg aaagcaatgc ggagatgtat cgggtagaag atacgatgag
    17221 tcgaattgct ttagcttctg gaaatttccg tttggtcagc tatgtcactc aaaccggtct
    17281 ctttattggt cttgatcgta cctcgaccat ccgaatggaa caaattacca accgttcgat
    17341 taatttagaa aaagtagtaa aagtaaatga tctttcacgt aaatatgttg cttcagagct
    17401 aacattagaa gaactttact gcgaactgca agcaatcgac cgagaccgtt ctttctttcc
    17461 tgtctggttg caaatcgtta gcgcggcagt gatcagtgga acaattatgg tgttatttgg
    17521 cggaaagatg accgatttac ctgtgacttt agtcattgga ggattgggat acgcagctta
    17581 tctatatagt ttgaaatttt ttagaatcaa atttttatcg gaatttttat cttctttatt
    17641 gattggcata gccgctatct acagcgtacg ttttggctta ggaagcaatc aggatctaat
    17701 cattatcgga tgtgtgatgc ctttagttcc tggagttcaa attacgaacg ctatccgtga
    17761 tttgctagct ggacattatg tctcaggagt ttctcgcgga acagaagcga tgatgactgc
    17821 tacgatgatc ggttttgcga ttgcttttat tttccagctt ttttattagg aaaggaacac
    17881 aagtatgggg acgatattta ttcagttttc ttttagtttt ttagcgactg cggcatttgc
    17941 cgttattaca aatgtaccta ggcgttcatt gatttgttgt ggtctttctg gtgcttttgg
    18001 ctggatgatc tactggatct gtgttcaaat aggcggatct gctgcttttg gttcattgct
    18061 tggttcttta ggagtagcat tcatcagcga tcttttctct aaacgtttga aaatgcccgt
    18121 aacgattttc aatattcccg ggatggttcc tctagtccct ggtggtttag cttatcaagc
    18181 ggttcgaaat ctcgttactg aatcctatcg agaagcggct cattatgcgg tccaagcaat
    18241 tatgattgct ggagcaatcg ctttaggtct tgtattatca gaagtcttta accataacat
    18301 ccgtaatttc aaagtaaagc gtgacgcaat catgatgaaa cgaaaaaaac ggctttttaa
    18361 ataaacgaag tgtaacaaag aaaaggttgt gaaagagacg ttcagccttg agcgatgaca
    18421 aaatatctca caaacattgc gacaagtaac cgcaggagca ataagaagta attatgagta
    18481 ttaaaaaaag ttttgtaaaa atagcttctc acatttttac ataaaattga cttaatactg
    18541 agaaactgac ttttgaacac cctctatctg taaaaaaggg gctgcgacat gatttttgtc
    18601 acggcccctc ttctttattc aaattttttc caaaacgttc gttgataata aacgatccat
    18661 agagcggaaa gtattagaac taaaccgatc acaggaatat tcactaacca aaatggataa
    18721 aacaaagcca acattccgta taagacgata agaataatac tgatgatcaa tgaacccatc
    18781 atagttgcga ctaaacccaa acttcctgaa cctctgttaa ataattggtt aatattggtc
    18841 cagttcagca gcagcaaccg ataatctcta gcgaaataac gtagtgacaa taagtagcag
    18901 ccgaataaag aaccaaatag caagctaggg ataaaaaaca aaggcagctg aaaagacagt
    18961 ccgcttaata atgcgatccc accagttaaa aaactctgca atatccatcc gaatctgaac
    19021 ttttctttta aatattggtt catagagatt ggcaaagaac ggataaacag aaaattttct
    19081 tgatccaaag aaataagatt gctgataaaa gaagtctgat taactgttaa gaaagccaat
    19141 gcgatcccta ccaggaaaac aacaccgatt aacttattat ctaaatcagt taattttatt
    19201 tcgcctccta gggcaaaagc aagaataaaa atgattggag ttaacaagga agaagaaaga
    19261 acttgcataa tcaaattagg ttcttttact aattgagcat tgtaactaaa taaaagctga
    19321 tgcacatttt ggttcttttt atgggttctt tttattttga actcactgtt tgaatcagtc
    19381 agttgctctg taagctttgg tactatcagc tgttttacag caaggaaaaa tcccagagcc
    19441 gtgatagcta gtattcccca acccaataaa ccttgttgag aaagtggctg actggcagca
    19501 tcatagaatg gcaataatat cgaaatcgga tgccgatctg ttaactcatt ttcaatagtc
    19561 cccgtttgat gattcatcca catgattcca gcaaatgcga tcagcatcga gctgccaagc
    19621 aagatactag aaactgtttt tctatgtttt ttaaacactg ctgttcgagc caaaccaaaa
    19681 acaatcaaac tagccagaca aaaaagaata atcaaaaata gagaaaacaa aaggattgag
    19741 ataatgatag taagcaaaat ccctagatca gctcgccatc cggttaaaat aaacatgact
    19801 agaagcggaa aaacaaaagg aatgatggtc aaagcaacta ccaaaatctt tgctgaaaaa
    19861 acctgttctt gtccaaaagg cagaggcaaa aatacttgca ggtcatttcc ttcaaaaaag
    19921 atattataaa tgactgatat actttgagtt aaacctaaca taccaaaaag tgccacgtaa
    19981 taagtaaaga aacctggcaa tttaacaaag ttcatagcaa acatagcaaa tccgtaaatc
    20041 agtaaaaaaa tcagccctga aaaaagattt tgagacaaaa gatagatagt taatcgcttt
    20101 cccttttttc ctttctctct tgctttattc gtgagctgtg gattaacata taaaagatta
    20161 actcgaatta attctaataa ttgtcgctta tacatacggt ccatctcctg tcgcttgcct
    20221 tccagccatt ttcaaataaa tcttttctag cgtttcatca ggtgagccat tcaataattc
    20281 actaactgcc ccctgataaa tcaattcacc tttcttcaaa atagccagtt tatcgcataa
    20341 ctgttgtgcg gtatccaata catgtgtaga aaaaatgact gtttttcctt ttgcggcatg
    20401 agccttcatc atttctttca aatcaaatgc tgcttgagga tcaagtcctt gcaaaggttc
    20461 atccaatacc caaatatctg gatctggcag caacgtgccg atcaaaatcg ttttttgacg
    20521 cattccatgt gaaaaacttg cgattgtttc attttgatga ctatacatat caaaaagcgc
    20581 tgtcaattct gctaaacgct tttctttttt ttgtgtgtct aattcatagg cagctgaaat
    20641 caagtcccaa tattctccag ccgtcaattg taaaaaaata tctggggaat caggcacata
    20701 accgattttt tgtttgatac tcaatcgatt ttccgtcaat ttcattccat ctactaaaat
    20761 cgttcccgat gatggttcaa taatactaac taaactttta atcgtagttg attttccggc
    20821 accattatgt cctaaaaatc caaaaatttc cccttgtctt atcgagagag acaagtcttt
    20881 taatgcttgt tttttcccgt aacttttact tacattgatg aattcaatca tttctgtact
    20941 ccttttgttt gttattactt tttattgtac ccttcgttat tggtggagta aatgtcaaaa
    21001 agagtgcata acaaaaaagg atagaaacga ctaggctgct acttgggttg ttctcttcta
    21061 caggaaaaca ctcttctaac aggtaagata tgagtctttg ttttctttaa aatgattcat
    21121 tttctatctt ttcttgcata aaattcttta gtttatcaat acttctcttt cccatctcta
    21181 gttgttctcc atttgcaaat gttactattt cgtttactct gctaatagat gtaatgtttt
    21241 cagtattaat gatataagct tttaaatctt tacataaata ctcatattcg agtgattttt
    21301 tatatttttt aattgtgccg tttacgagaa tctcttcatc tttcaatact aggcctattt
    21361 tatttttata acctggcatg ctcattagat aaaggatttc atcgtaatgg atatagtgat
    21421 cgctattacc ataacggaca gttgtccacc tttttttcgt agcatgttga acaggatagt
    21481 tttctttttc tttcaataag taactttgga tgcttttttt atttttttca taagtgaatt
    21541 cttttatcaa ataaccacta ggcgtaatct ttcgattaat aacagttatt gctttattcg
    21601 gatgagacgt tagataaaca attttacact tttcattctt ttctctaagc atttcgccta
    21661 gttcgatacc gctataatag gcgttcaaat cgatgtctag gaaaaaaata tctgaggaat
    21721 agatttcatt atttttaatg aattcaacaa attgtataac gttcaatccc tgaacgatct
    21781 caaatttcca aggacttaat aaaattctgg attcttccaa aatttctact agcatttttt
    21841 caaaaaccaa actatcatcc aaaataaata tacgcgttac cataaaaagt acctccactc
    21901 gatattttac tatcaaaatc atttcataca acttttcaac atacatgtta ggaataaatt
    21961 gtaattctct tcacaatctt ttccaacacc tttgaaaccg atatcaaata ctttcaggat
    22021 gctgcttcta aaataaatat caaacaaatg aatacatcat gcaaaaaaag ccagacaacc
    22081 gacgtccttg cgtgtcagct gtctgagcta tgtttattta ttttgttctt ttaaaaagaa
    22141 cgatacacat actgattttt cggagcagag actttctcag cgtttgtaac aactacagat
    22201 gggatttcac gcttgaatgg ttgataatag tctgattgga tggttccttc tattttatac
    22261 cattcatcat ttttaaccgt tactccttcc ggcatatgcg tcaacaaacc aaagactcct
    22321 gaatctgcca cacaatggat aatgccgaat cggaacaaga agatatctgt attgctatcg
    22381 ttcggtgttt ggtaaacaaa tccttcatag ctgatttttt tgccaatgaa ttcgctaggg
    22441 taattataaa ttagctccat aacttctaaa tagttttcat ctgtgatctt gatagtttgc
    22501 ttatccccat acttttcttt caactcattc atttggtcta agtagtctga tttgttgaaa
    22561 taaatactgg tatccggctt taagtattgt gtctgcatct ccggatcccc tactgattct
    22621 ttacttactg ggaaattaaa tcctttggct tctacgatcg tagtgtcgag actaactgtt
    22681 ggaaacaacg tccccacgat tactggcaaa gctagcagta cataagcgat tccacgttga
    22741 taaggttttg acaacccatg gtcatgatcg tgctcgtgtg ccttttcttc tgcttttccg
    22801 tttttgaccc acaaatacag ctgaactaaa gccaaaagag cagataaaat cattgaaaga
    22861 atagctaagt agcgataatg gacattgata tattgatcga gtttgccaga aacttgcaag
    22921 tacatcatca attcgaaata cccagataaa atcaaaaacc gcagcatatt ttccctccta
    22981 aataaataat gtgaatccaa taacgctaag ggcaatcagt ccgatcagcg ataaaataaa
    23041 gcgtcctgta aaatatcgtt tcatcatcag tagatttttg atatcgacca ttgggccaaa
    23101 gaccaaaaag gcagctacag gtgctgtgcc gaacagactt aacaaagaag atcctataaa
    23161 cgcatctgct tctgagcaaa gagacatcgt aaaggccaat aacagcatga tgataatggc
    23221 taaaatcttt gttgagccga gcgttagcat aattctagtt ggtaaataag tctgcatcgc
    23281 agctgcgatt aatccgccaa tgatcaaata acgacctgta tcaaaaaatt cgtcgattga
    23341 atgtgtcaac acatgcccag tttgatggat cagctgattt tttggttcat tcttctcact
    23401 ttgttcttct tcatggacat gatgatggtg attttctgct actttagacg tgattttttc
    23461 tttcaataca gcgtcttttt gaaaataggc aagccagaca ccaatcacaa gggcaacaaa
    23521 aaagctgcct atcacccgcc agcttgccat ctgccaagaa ttgccaaatg cgataaaagt
    23581 ggaaaaaatc acgattggat tgatgatcgg agcagtcagc ataaaagcaa acgctgtgtg
    23641 aactggtacg ccttttttaa caaattgatg aacgatcggt acgatgccgc attcacatga
    23701 aggaaagaaa aatcctaaga tcgaaccagt aaaaatagag aagatacgat tttcaggaag
    23761 ccatctcttc acacgttctg gggtcaaaaa tacttgtaag gcgcctgaga taagacagcc
    23821 taataatacg aaaggtaatg cttcaataac gatcgataag aaaatcgtac tcatctgcaa
    23881 aaccgaattt ggaataaaag aaaacatgag tttcctcctt tttatttcta gttcattcgc
    23941 tttgcaaata tacgctttta ttcaaaaaaa acaagttgct ctgtaaattt ttacacaact
    24001 cttatttttt accaaagcac ttcaattgac tttttctgtt attcccatta aattaaggtc
    24061 tcatacatat cacttctatc ataaaaggga gaataaaaat gaaacaaacc actaacttga
    24121 ctgaagttca agacatactg caacagtcat ccgttgccgt catttatctt tcgatgccta
    24181 attgcagtgt ttgtcacgct gttcgtccac gactagagga acttctcaca cattatgata
    24241 ttccggcact tcatttggat gcatttgaaa caccagaagt agctagtaaa tttgaagtgc
    24301 tgactgcgcc agttgtcctg atttttcatc aaggaaaaga agtctttcgg caagcacggt
    24361 tcatcgactt taaaaaaatc cgttatctac tagatgaact gacagcagaa gatgattccc
    24421 tttcttatga agaaatcttc cgaacaaaat aatcgttgtc caaacagaaa aaagcgattg
    24481 aacaagtgaa agttttgcct tcatttgttc aatcgctttt tatttttatt ctgctgtatt
    24541 ttcggaatgc gcatttgcgt cagaatgcaa gaaatagatc aaggtttgca attcattcgt
    24601 caaatcaaca ttttggatca atacatctga cggagcattc aatcgagcag cggtaaagtt
    24661 caagattcct ttgattcctg cttccgttaa ctgattgact acttcttgtg ctctttcagc
    24721 gggtatcgtt aaaattgcta cttcgatttg ttggatcttg atttgttctg acatatcttc
    24781 cattggataa acggggatac cgtctacgat gcgcccaacg atttcctcat tcacatcaaa
    24841 agcacaactg atacgtatgc tattactttg atggaatttg tattttagta aagcacttcc
    24901 caagttccct acaccgatca gcgcaacatt tgtcaattga tcgtcgttta atgtctttgc
    24961 aaaaaagtgc ataagatctt ctacatcata accataccca cgttttccta attcgccaaa
    25021 ataagaaaaa tctcgacgga tcgttgcact atctacttga acggcttcac ttaattcagt
    25081 tgaagacact tttttctttc cagcattatt taaaatcctt aaatagcgat aatacaaagg
    25141 cagtctttta gctgtcgcct ttggaataat atgatctttc acgaaatgac ctccacgtat
    25201 tgtctaatta catctttgat ttctccatca tagcagtatt tataggaaaa aatcaattcc
    25261 attgctcaga tattgtgaaa ttttattctt tattcattta tttatcattt ctgcaaatct
    25321 cgcagtataa tggaatttat ggtagactac taattgaaaa atagtttttt atttgaggag
    25381 aattcacaaa gtagaaaaga ggattccatg atattattac aagccaatca gattgctcgc
    25441 cattttggtg cagaagtctt atttgaaaac atacatttag aaatcagtac aggcgctcgt
    25501 gtcgctttag ttggcagaaa tggcgctgga aaatcgactt tattaaagat cattgctgga
    25561 attgaagcac ctgatgaagg aaagatcgct aaaaataaaa cagcgactct cggatacctc
    25621 gctcaggaca caggacttga ttccaatgaa acagtttgga acgagatgct gaaagctttt
    25681 gaacaagtta gacaaatgga agagcggatg cgcgagctag agattttcat tagcgaaggt
    25741 acgccagaca cacctgctta tagttcttat ttaaaagaat acgaccggct gcaacacgat
    25801 ttttcagatc gaaacggcta tggttatgaa aatgaaattc gttccgtatt gcatgggttt
    25861 aaattcgatt cttcatttta tgatcagaaa atcgatactt tatcaggcgg acagcgcaca
    25921 cgtttagctt tagctcgcat gcttttgcaa caccctgaca ttttgattct agatgaacca
    25981 acgaaccatt tagatatcga gacactttcc tggcttgaga attatctgca aagctatact
    26041 ggtgcattat tgattgtttc ccatgaccgt tattttttag ataaagtagt caatgaagtc
    26101 tatgaattaa gcagaagaaa aatgcgccat tacaaaggta actattcgaa atatctagat
    26161 ttgaaagcag aacagttagc aagtgaatgg aaggcttatg aaaagcagca aacagaaatc
    26221 cataaattag aagacttcgt tgctcggaac cttgttcgcg cctctaccac aaaacgagcg
    26281 caaagccgca gaaagacatt agaaaaaatg gaccggctgg atcgtcctca aggggatgaa
    26341 aaacaagcaa actttctatt cagtatagaa aagccttctg gaaatgtagt cttgcaaatc
    26401 gaagatgccg ctatcggata tgataatgtc accttatctg aaccaatcaa tctagatatc
    26461 cgtcgtcaag aagcgattgc tctagtaggt ccgaatggta tcgggaaatc gacgttgctg
    26521 aaatcaatca ttcaccagat cccttttatc cgtggaaaag aaacgctagg gacaaatgtc
    26581 accatcggct attacgatca aggtcaagct gatctgcata caaacaagac gatcctagca
    26641 gaattgtggg atgagcatcc taccactcca gaaaaagaga tccgaagtgc tttaggtgct
    26701 ttcttatttt ctggcgaaga tgtagaaaaa ccaatccctc tgctaagtgg cggtgaaaaa
    26761 gcacgggttg ctttagccaa gttagcgatg aacaaagaaa attttttgat tttggatgaa
    26821 ccaacaaacc atttagatat tgacaacaaa gaagtattag aaaatgcatt gatcgattat
    26881 gacggcacac tgctttttgt ttcccatgac cgatatttca tcaatcggat cgcgacaaaa
    26941 gtcgtcgaac tctctgaaaa tggcagcaaa gtctatctag gagactatga ttattattta
    27001 gagaaaaagc aggaagaaga agaaatcgcc gaattgttgg cacaagatga gccagaatct
    27061 gctcctgctc ccacgaacaa atctgattat taccaaagca aagaacagca aaagcttatc
    27121 cgtagccttc aacgaaaaat tactcaggtt gaagaagaaa tggcgcggat cgatgaatgg
    27181 atcgatcagt tggaacaaga aatggtcgcg ccagaaaacc tggaagatca cgtaaaactc
    27241 aatgagctga accaagattt agaagctgct agacaagatc aagaaacaaa attgacggaa
    27301 tgggaagaac taagcttgga attagaagaa tatgaaaatt aacgaaaaaa ccaaaacaga
    27361 aaccattcat gctgttttgg tttttctttt tttattttat caaacaaaaa ttccatattt
    27421 acctttttca ccaataagaa gaccaattac tagtcattct cactaaagct tctaaataat
    27481 agtaatcacc ccaaatacaa ggtgtattta ccccattatt tttgggctta ttataaacag
    27541 actgtgttaa aaagccatct tcttcttccg ttgtagcata tctttctttc aattgtcgca
    27601 taattttcca aacagcagac tggtataagt tcttttctgt tttgtctaat agacctgatt
    27661 ctattaattc taataatcca caaaccatga tcaccatact agatgtatct ttttcttctc
    27721 tgctagaatc atcaaagatc aaatcccaat aacaaatggt atcttctggc aaccgattca
    27781 taaaataatt ggctaatatt ttgcttaaat caaaatattt ttgttctttc aaataacgat
    27841 aacttaatgc aaaaccgtaa atcccccatg cttggcctct agcccaacag gaattatcac
    27901 tataaccttg atgcgtactt ccatatcttg gttttccatc tttgacatcc atataaaatg
    27961 tatgataagt tgaaccatct tctcttacta aataatctgc tgcattttga atatgagcct
    28021 gagcagcttt tttatatttt tgatttccag tttcttcact cgcccaatac aataatggaa
    28081 gattcatgca acaatccatg atcattctac cttgttgtga aggatcacta ggatctcccc
    28141 aagcttgtat aactcctgcc ttctcccaaa aacgctgaag taatgtttct gcagcatcaa
    28201 ttgaagcttc cttcattttt cgatttcccg ttattttata atctgctacg gttgataaag
    28261 aatacaaaaa accaagatcg tgatgattag tgataatttg acagtcaatc ctgtggataa
    28321 aatctatatc tgctttttca gctgtttgtt gaaataaagg atcacccgtc ttctcaaata
    28381 acaaccaatt aattcctgtc caaaatcctt ctgtccaacc aatattgtct cttaatggat
    28441 aacgtccttt tataggacac tcgcttggga attgatactg actgttactg atacgtttga
    28501 ttaacctctc cgtgtgtctg cgacaacgct cgatctcatt attccattct ttttccatta
    28561 attctttata acattcattt atccattttt ctggatgtat tatctcttca aacatcactt
    28621 tatcaagcat ttccccacca gcctttagca taattttagt ctttttttat tgtttttatt
    28681 ttggatagtt agcccggttg tcccatcaaa atcaattttg ggacgattgt tccatacttc
    28741 taaactataa tcccgatctt gatgagcaac cactcctgtt ccgagccaat gggttccttt
    28801 tccaaatgta ccctctaatc caggaataaa agtgttttcc catgtcatca agtttgtatt
    28861 gggcaaaccc ataatagctg tggattttcg tttaatagta tcgtcactta aacagataat
    28921 acctgaaaag ccagcttcac tcaccctata gttttccttg tcttcttcta tgattattcc
    28981 ttctttctca ggagaacatt caatagcaaa ccctgtctca tatagaacat attctgtatc
    29041 taattctact ttgtgtacgc gaatctgcca gccttcaaaa ggaataagcc aagttctgat
    29101 ttttgcctgt aaagtaggtt cccatgtaga ggcaagtacc tctttcttac atacaatgtc
    29161 tcgtacatta tttcgaacga tgaactgtcc ataggttttg atgctctcat tcttaaaact
    29221 ggtcgtgata tatgtgtccg ctcgctgaat ccctagcatt gaatccattg ccatttctgc
    29281 tttcaattga ttcgctcgcg gtatcgaaaa accaaattga ctagaataag caaattttaa
    29341 atatttttca ttggtcaatg tatggaaatt tggccctaat tgtcccgcat tcaaaaaaaa
    29401 tgtatgtcca ttatcatgtt gtatgagcaa attggcttct gaaagaagtt tagttccttc
    29461 acgaataggc ataggcattt ctttagccgt ccaatactca tggtcttcag gtagcgccaa
    29521 caataaaaaa atcttattca gccataatgg agataacgta ctattatagg gttcagtcaa
    29581 tgtcaattga gggtaggtat aaccaatatt caggatacct ttttcatcaa atatttcttt
    29641 ttgcatccac catcttatat tccgattgac taaccccttg atttcaccta atttaaaagg
    29701 caaaagatcg gcccacaaca tagctgacca aaagcttgaa actgcaaaac gataaatcat
    29761 gcttcgacca aaaggcacat ttgcaccatc ttccgcaaaa aaatgaatat agtctctaga
    29821 aaaatcaata gctcggttct tatatatctg gctaatgggc ttttctggtt ctagtacgct
    29881 ataaatcaaa ccataataat gaaatgcaaa aggattataa taatcttggc gacctcttga
    29941 actgtctcga taccagccat tttgaagata acaattttct atattatcaa atgcttcttt
    30001 cagtgctgat tctttaactg gataaccaaa tttttggata attgttccga caattatctt
    30061 aaaaaattgc caattaccat ctgcacattc atattcaaaa atttgcgaga accaatgaat
    30121 aatctggctt ttttctcctg tatctaattt tttccataat aattgttcat gatgatataa
    30181 cgctaataac attgcgggca tttctaccat acgctggtct ctttcatgta tttctcccca
    30241 ataatcggga tgggaaggat ctgttccagc ttttattcct gatagaatta atttcaacca
    30301 tcgttcatct aattggtcaa ttgcgggccc cgctccccat aaaacccgag agaatccttc
    30361 cattcctgca attttatttc catatcctac cccttgatgt atataatcaa tttgtgcgcc
    30421 acttgctcga taaaacggct ggagtggttg atttatcttt ttaaataagt caaagtaatc
    30481 ctgtctggtc cttaatagtg gtactggatg tttcattgta ttttctcctc tttttactac
    30541 caaatagcta attccgactg ttctaatcga agtaagcctt caataaaaaa ataatcagaa
    30601 tagatcagtg gcacattata agtcagttct ctatggtatg cttctgctcc gcctccaatg
    30661 atcccatctt catccatatt ccaatttgca tagtgctttt ctgtcttttg gagtatattg
    30721 atagcagctt catgatacat ttttgatcgt acagaggaaa catatcctgc taattccaga
    30781 agaccgcatg cagcgcatac tccagcactg gtatcaatat tcgaatcttt tacagcaaaa
    30841 tcaatgagag gaatataacc agttttttcg attttctgaa taaaatttac tgctatcttt
    30901 tctgctatag ctagatagta cggatttttt gtgtaacgat aacttaacgc gaatccatat
    30961 aaagcccatg catttcctct gctccaacaa gaattctcat tgtatccttg cccgccaagt
    31021 aaccgaatat atttccctga ttctgcatca aattctgcga tatgcccggt tgatccatct
    31081 ttcctcacta gatacttagc tgttgtatct gcatgctgcc gagcaatttt agagaacctc
    31141 ggatcaccag tttccctcga agcccaatac agtaatggta aattcatcat actatcaata
    31201 atcatccaac caggtttatg gtcattccat gctactaaat agttccctga aatattatag
    31261 cgtccagcaa gtaccgtagc tgcatgtaac cctagttgtc gactatgttt gttttgagac
    31321 tgttgataat gtgcaatagc cgatggtaag aataaaaaac ctacatcatg atgaacgtct
    31381 gaaaaatggt acagggcaga ttctaataat tgttcttgtt gtattgctgc ctctttgaac
    31441 ttttctcgtt ttgtcttgtg aaagagctgc cataaaatac cactccaaaa accattggtc
    31501 caccagtcga ttcctctgtc ctttattacg tctgtatatt ttccgttgta agaaatatat
    31561 ggaattttat tccccattct gtctaattcc gcatctatct tttcagaagc tacattcaaa
    31621 atttttcttg accattcctt cgtctgttca ttcatggata tcccgccatt cttttaattt
    31681 ttatgaatgt ggagttgctc ctagaacgaa ctagatggtt aacattcgtt atttttttta
    31741 cttatttgca gctccaatgt cattctcctt aaatttagac tgcgcccaca cataaatcaa
    31801 gaccattggg atcactgaag ttgctaaaat tagcataatt gcgccataat taattggctc
    31861 acctgtacta gaaaccgtca tatttttgat aattaaggga accgtatatt tatctttatc
    31921 tgaaagcatg actaatggaa atagatattg attccaactt cccataaaga taagtaaacc
    31981 actagtaata atcgtaggtg ataaaatcgg tacagcgatt ttcgtaaaaa tcacccactc
    32041 attggcacca tcgattctgg cagcctccat cagtgctgta ggaaattgac tgctatattg
    32101 tcgcatcata aacactgaag ttgtactggc aatcgaaggg atgatcacac ctgcataagt
    32161 attcgataat cctaaacgac tcataatgta aaataaagga atcaatgtgg taaaaaaagg
    32221 caccatccta gatagcataa ctgtattgaa caacagttta ttccccataa aacgatactt
    32281 tgctaatgca taacccgcca ttgtagaact ggctgtagct aaaactgtac tgattacagc
    32341 aataacaatc gaattccaaa taatcaaggg ataattatat ctcgccatga tggcttgcca
    32401 gttttttaat agcatatccc caaacttgaa attgattccg ccacttaaaa tttcttgatt
    32461 actttgcgtt gaagctacaa tcatatataa atatggcatc aaaaatatta aggcaccaaa
    32521 cacaataatg acgtacgtaa aaattctgct ttgtatcttt tgtttagtca tttttatcac
    32581 ctccgaattt aaattgaata gccgataata taacagatac aaacaccatc gtccaggcaa
    32641 tagcagaggc ttttccaaaa tcaaccattt caaaacttgt tttatataaa ttgactccta
    32701 cgaccaaagc tgaattttct ggtccattgg aagtcccaaa gaatatattt gggatattga
    32761 aattacccaa gccattgata gtggctaaaa ctgtggccat taaaatgatt ggctttagca
    32821 tggggatcgt taataaaaag aattgccgaa atttcgatgc tccatctatt tcagcacttt
    32881 cgtatatctc tttagaaatc ccttgaagac cagctaaaaa taaaatagtg ttatatccta
    32941 cactaagcca aatcatcgtg atgatcattg atattcgtat ccaaaaagga tttgttaacc
    33001 actgtacacc gttaattcct agcgcattca aaaatccatt gaataaccca gtcggattga
    33061 aaaaagtcat gaatacggaa gctacagcca ccgttgatgt aatggttgga atataataga
    33121 cagttctaaa aaatcctttt ccttttaact gtgctgaatt caataaaaca gcaaagtaaa
    33181 tagctaatag catcgttatt ggaattgatc ctgccatgat aatcaaaaca ttcgtgtaag
    33241 atttaataaa caatggatct cggaagatag ctagataatt gtcaaatcca acaaattcaa
    33301 atgagtagcc aattttcata aaactaaaag caaacgagat aatgatggga atcaaaatcg
    33361 tcacaataaa gaaaataaca aaaggtgtga ccaatccata agcacttttt ttatgagacg
    33421 tatctgttgc ttttttcttg ctgttcttta ttgtcttgct tggtaacgaa gattgcatca
    33481 tcgttaagtc ctcctaatat tttttaatca ccagttaatt ttacgccgta cttatgagca
    33541 aaattttctg cttcttttag atatgaattt tcaaaatcac ctgtttgcca atagttatat
    33601 gcagcggtag tcaaataaga cgtagcatct gaataagtgg ctggtaaaac agtatttgat
    33661 gtattttcat tgattttatt caatagttca tagtattttt gtcctcctta gtagtcaaac
    33721 tttttctcag ctgcttctgt ttgataggcc aatgagttag atgtagccac gcccagctcc
    33781 agtgcgttgg ctaagcattc ttcatctgtc agcataaatg taagaaattg ttgtgccact
    33841 tgtggatttt tggcatcttt agggacataa aaacttgatc ctcctgatac tgcacctttt
    33901 cctggatttt cttttgaaaa aggaatcagt ggagccatgc gccatttgtc agatgcatca
    33961 ggaaaattca aagacatatt cgtagcatac cagccgccag caacaaacat tgcagattct
    34021 tgaaatgctt tctcagcatc ttgtgcaccg tagaaatgta caatatctga gtcgatcatt
    34081 tctttgatta actttgcagc atgtttcgct tctttagtat tcagacggat atttccttct
    34141 tcatcaagca aagattgatt ctgctgggct agcatgttga taaacaattc ttggtcccca
    34201 gattctggca aggcaattcc aaaactacca gttttttctt ttaatgcttt tgccacctgg
    34261 atatattctt cccaagaact gatttcttca aaatctacac ctatttcagc aaattttttt
    34321 ggttgataat aacaaagtgc tggggtaaaa tcactagccc aaggaataat cgtttcatta
    34381 ctcgtttgtt gacgcaaaat attcaatttt tgatcataaa actgatcgcc atatttgtca
    34441 taaaatccat attcatcagc actataaaaa gcatcttgaa atttttccag cacacctgct
    34501 gctgaagtgt ctgccatgga gataatatcc ggcatatctt cgtgtgttac caattttggg
    34561 gtgatcattt gtactgttgc tgttcccgaa gctacattca caatttttaa gtcaatattt
    34621 gctccagttt tgcggttata gatttttgca gcattttcaa catagtttgt cagtttgccg
    34681 tttgcgacat atacttctaa tgtagtggct ttttttatca ttggatctgc gatgtttgct
    34741 gttacttctt cgttagtttt tccacatgca cttaagctga ataatagggt aatgtttagc
    34801 aaagctatca ccaatttgta tctttttttc atgtctatcc cttctttctt ttcaagttc
//
LOCUS       DACENJ010000004        34680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.004, whole genome shotgun sequence.
ACCESSION   DACENJ010000004 DACENJ010000000
VERSION     DACENJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.004"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1152
                     /locus_tag="IW748_000132"
     CDS             <1..1152
                     /locus_tag="IW748_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103068.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IW748_000133"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IW748_000133"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1103069.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IW748_000134"
     CDS             2728..3003
                     /locus_tag="IW748_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1103070.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IW748_000135"
     CDS             3009..3776
                     /locus_tag="IW748_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1103071.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRIYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IW748_000136"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IW748_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1103072.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IW748_000137"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IW748_000137"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1103073.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGRNKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IW748_000138"
     CDS             7920..8222
                     /locus_tag="IW748_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1103074.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IW748_000139"
     CDS             8225..9388
                     /locus_tag="IW748_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1103075.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IW748_000140"
     CDS             9414..12842
                     /locus_tag="IW748_000140"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1103076.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IW748_000141"
     CDS             12863..13987
                     /locus_tag="IW748_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103077.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IW748_000142"
     CDS             13977..14414
                     /locus_tag="IW748_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1103078.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IW748_000143"
     CDS             14398..14736
                     /locus_tag="IW748_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1103079.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IW748_000144"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IW748_000144"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1103080.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IW748_000145"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IW748_000145"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1103081.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IW748_000146"
     CDS             15893..16936
                     /locus_tag="IW748_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1103082.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IW748_000147"
     CDS             17095..17823
                     /locus_tag="IW748_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1103083.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IW748_000148"
     CDS             17939..18184
                     /locus_tag="IW748_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1103084.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IW748_000149"
     CDS             18181..18399
                     /locus_tag="IW748_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1103085.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IW748_000150"
     CDS             18535..19074
                     /locus_tag="IW748_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1103086.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IW748_000151"
     CDS             19067..21340
                     /locus_tag="IW748_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1103087.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IW748_000152"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IW748_000152"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1103088.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IW748_000153"
     CDS             complement(22513..22767)
                     /locus_tag="IW748_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1103089.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IW748_000154"
     CDS             complement(23167..23436)
                     /locus_tag="IW748_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103090.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IW748_000155"
     CDS             complement(23906..24361)
                     /locus_tag="IW748_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1103091.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IW748_000156"
     CDS             complement(24499..26427)
                     /locus_tag="IW748_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1103092.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IW748_000157"
     CDS             complement(26624..27457)
                     /locus_tag="IW748_000157"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1103093.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IW748_000158"
     CDS             complement(27649..28353)
                     /locus_tag="IW748_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1103094.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IW748_000159"
     CDS             complement(28376..29002)
                     /locus_tag="IW748_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1103095.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IW748_000160"
     CDS             29328..31703
                     /locus_tag="IW748_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1103096.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IW748_000161"
     CDS             complement(31727..32218)
                     /locus_tag="IW748_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1103097.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IW748_000162"
     CDS             32405..33325
                     /locus_tag="IW748_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1103098.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IW748_000163"
     CDS             33479..33823
                     /locus_tag="IW748_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1103099.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IW748_000164"
     CDS             complement(34013..34513)
                     /locus_tag="IW748_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1103100.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT        11463 a         6067 c         7143 g        10007 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tatacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtaga
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttgtcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
//
LOCUS       DACENJ010000005        34279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.005, whole genome shotgun sequence.
ACCESSION   DACENJ010000005 DACENJ010000000
VERSION     DACENJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.005"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..772)
                     /locus_tag="IW748_000165"
     CDS             complement(<1..772)
                     /locus_tag="IW748_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1103101.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(769..1452)
                     /locus_tag="IW748_000166"
     CDS             complement(769..1452)
                     /locus_tag="IW748_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1103102.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(1619..2506)
                     /locus_tag="IW748_000167"
     CDS             complement(1619..2506)
                     /locus_tag="IW748_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1103103.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(2503..3372)
                     /locus_tag="IW748_000168"
     CDS             complement(2503..3372)
                     /locus_tag="IW748_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1103104.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(3509..4795)
                     /locus_tag="IW748_000169"
     CDS             complement(3509..4795)
                     /locus_tag="IW748_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1103105.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5068..5646
                     /locus_tag="IW748_000170"
     CDS             5068..5646
                     /locus_tag="IW748_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1103106.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            5646..6575
                     /locus_tag="IW748_000171"
     CDS             5646..6575
                     /locus_tag="IW748_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1103107.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            6572..8206
                     /locus_tag="IW748_000172"
     CDS             6572..8206
                     /locus_tag="IW748_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1103108.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSINQLLSNAQHHYEQTIQD
                     EKKKRSLEIQTMQAQIQPHFIYNTLTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKK
                     EEVIPVKEEIKSVQSYIDLLSMRYGERISAKIMIPDELQSLSMPNMILQPIIENAYLH
                     AFHNKTQGFITVYGKTKNQLLLFEVIDNGDGFDTENTKQKNDHFSGIGISHVDERIRL
                     MYGDAYGLTIQSVIGTGTTVTICLPLLPKNQSEYQK"
     gene            complement(8269..9105)
                     /gene="pheA"
                     /locus_tag="IW748_000173"
     CDS             complement(8269..9105)
                     /gene="pheA"
                     /locus_tag="IW748_000173"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1103109.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9102..9611)
                     /locus_tag="IW748_000174"
     CDS             complement(9102..9611)
                     /locus_tag="IW748_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1103110.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(9604..10899)
                     /gene="aroA"
                     /locus_tag="IW748_000175"
     CDS             complement(9604..10899)
                     /gene="aroA"
                     /locus_tag="IW748_000175"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1103111.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(10912..12003)
                     /locus_tag="IW748_000176"
     CDS             complement(10912..12003)
                     /locus_tag="IW748_000176"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1103112.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12020..13186)
                     /gene="aroC"
                     /locus_tag="IW748_000177"
     CDS             complement(12020..13186)
                     /gene="aroC"
                     /locus_tag="IW748_000177"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1103113.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13188..14252)
                     /gene="aroB"
                     /locus_tag="IW748_000178"
     CDS             complement(13188..14252)
                     /gene="aroB"
                     /locus_tag="IW748_000178"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1103114.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14285..15307)
                     /gene="aroF"
                     /locus_tag="IW748_000179"
     CDS             complement(14285..15307)
                     /gene="aroF"
                     /locus_tag="IW748_000179"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1103115.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(15966..16940)
                     /locus_tag="IW748_000180"
     CDS             complement(15966..16940)
                     /locus_tag="IW748_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1103116.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(16921..18804)
                     /locus_tag="IW748_000181"
     CDS             complement(16921..18804)
                     /locus_tag="IW748_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1103117.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(18822..21047)
                     /locus_tag="IW748_000182"
     CDS             complement(18822..21047)
                     /locus_tag="IW748_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1103118.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21162..22196
                     /locus_tag="IW748_000183"
     CDS             21162..22196
                     /locus_tag="IW748_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1103119.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22263..23276)
                     /locus_tag="IW748_000184"
     CDS             complement(22263..23276)
                     /locus_tag="IW748_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1103120.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLIWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(23354..23542)
                     /locus_tag="IW748_000185"
     CDS             complement(23354..23542)
                     /locus_tag="IW748_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1103121.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(23982..24110)
                     /locus_tag="IW748_000186"
     CDS             complement(23982..24110)
                     /locus_tag="IW748_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1103122.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24113..25486)
                     /gene="rlmD"
                     /locus_tag="IW748_000187"
     CDS             complement(24113..25486)
                     /gene="rlmD"
                     /locus_tag="IW748_000187"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1103123.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(25597..26652)
                     /locus_tag="IW748_000188"
     CDS             complement(25597..26652)
                     /locus_tag="IW748_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1103124.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(26662..28092)
                     /gene="gatB"
                     /locus_tag="IW748_000189"
     CDS             complement(26662..28092)
                     /gene="gatB"
                     /locus_tag="IW748_000189"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1103125.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28089..29561)
                     /gene="gatA"
                     /locus_tag="IW748_000190"
     CDS             complement(28089..29561)
                     /gene="gatA"
                     /locus_tag="IW748_000190"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1103126.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGNPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(29561..29866)
                     /gene="gatC"
                     /locus_tag="IW748_000191"
     CDS             complement(29561..29866)
                     /gene="gatC"
                     /locus_tag="IW748_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1103127.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(29972..32008)
                     /gene="ligA"
                     /locus_tag="IW748_000192"
     CDS             complement(29972..32008)
                     /gene="ligA"
                     /locus_tag="IW748_000192"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1103128.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32025..34259)
                     /gene="pcrA"
                     /locus_tag="IW748_000193"
     CDS             complement(32025..34259)
                     /gene="pcrA"
                     /locus_tag="IW748_000193"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1103129.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
BASE COUNT         9452 a         7507 c         6166 g        11154 t
ORIGIN      
        1 atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt tcatcttttc
       61 gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca atcattgcaa
      121 ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg attgccacgc
      181 tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg ttatcaaaag
      241 ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc ccgtatcctg
      301 ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac aatcctactg
      361 ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt ctcccctcgc
      421 tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac cctactaaga
      481 accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc gcaatagcac
      541 tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg aaaaggatca
      601 acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt ttgcgcatac
      661 gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact gccaaaatga
      721 cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc attggaagat
      781 cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga ccattccaaa
      841 actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg caagggccaa
      901 gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag caatcggcat
      961 aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta tagccatcaa
     1021 ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa atactccagt
     1081 gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag atatatcata
     1141 tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga tcccgatagc
     1201 aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt tccctactag
     1261 atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca ttccgatcat
     1321 catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg gtgcaaaaac
     1381 aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca cttctttaaa
     1441 attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat gaaagagttt
     1501 tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata acacacgtcc
     1561 caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt gtgttttttt
     1621 attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa aatacgatgg
     1681 cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct ggtgtatagg
     1741 aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat tgcaaagcta
     1801 ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca gccgcggcat
     1861 aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct tttagaccgt
     1921 ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt cgaaagaaga
     1981 agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg ttgattccca
     2041 aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat gccgcaaaag
     2101 gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta ctttgataaa
     2161 tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg gtcaccaaag
     2221 cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa aacaaatttt
     2281 gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata tccacgggtg
     2341 tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat gataggatgc
     2401 ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat gcttgttttt
     2461 tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac tttctcctcc
     2521 tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa aggaaactgc
     2581 tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt attgagatag
     2641 ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat caaaaagctg
     2701 caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta aatttgggat
     2761 cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg cttcataaat
     2821 ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc cagtccaacg
     2881 ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca accagccaat
     2941 cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag attgagaaaa
     3001 tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa gaaagatagc
     3061 ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg aaataaaaag
     3121 tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat taaaaaaagc
     3181 ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt taaatgtcat
     3241 attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg gaataaaaac
     3301 catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt tgaataaatc
     3361 tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga aaagacaaga
     3421 aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc tgatgaaaaa
     3481 gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct acttgttttt
     3541 gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga agagcttcag
     3601 ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt atttcagatg
     3661 tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa gcttcttgat
     3721 aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct ttcgcaagag
     3781 tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc gctgaatctt
     3841 ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa gcttgcgctt
     3901 tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca gattggtttt
     3961 ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct actgctcctg
     4021 attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga tcttctttca
     4081 aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg atcgtgactg
     4141 tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc cttactcgac
     4201 caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg acatctcgcg
     4261 ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta taacctgctt
     4321 ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa ggaacaccgt
     4381 atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga tcttccttta
     4441 caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct tgtatcctat
     4501 aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct aaagcggtgt
     4561 tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct gtttcttcgt
     4621 tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca tcccacgccc
     4681 agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat cctgttaaga
     4741 aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg ttcattttta
     4801 tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata ccaaaatttg
     4861 aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa taaaaaaata
     4921 caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg aaatggtgag
     4981 gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt tttaaattac
     5041 aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat cgatgatgaa
     5101 cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg gtttcaatta
     5161 atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca tgcaatcgat
     5221 atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc gcggctatta
     5281 aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga atttgatatg
     5341 gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg attgagtcct
     5401 gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa gcccaacaaa
     5461 agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca gattgctgga
     5521 caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt ctgttatata
     5581 ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg atcacccatt
     5641 cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt tcggaagaat
     5701 ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt caaaagtttc
     5761 gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca atccaagata
     5821 tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc tatcataagg
     5881 gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac gagcagcaag
     5941 caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc aaagaacttg
     6001 agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg aaacatgagg
     6061 caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt gggaatgaag
     6121 aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag tatttcgaag
     6181 atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct gcaaatgcaa
     6241 aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa aattatgaga
     6301 aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc tatctctcag
     6361 ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa gtccgtatca
     6421 gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc tgtgagaaag
     6481 tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca ggaatgacac
     6541 cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag agcatgaact
     6601 ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca tcgtcatttc
     6661 tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc aatcgaatga
     6721 tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa tcgagaatac
     6781 attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg atccagccca
     6841 acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact ctctttcaaa
     6901 aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa gaacttttgt
     6961 cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg cgattgctca
     7021 gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg gattgactct
     7081 tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata cattgaatca
     7141 cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc tttttcaatc
     7201 tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga cgatcatcgc
     7261 atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac taacagtagg
     7321 tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc gccaggattg
     7381 tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg tgatttttcc
     7441 gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat ttatgtattt
     7501 ggatcgacac acaaaatcga tcaatcaatt gctaagtaat gctcagcatc attatgaaca
     7561 gacgatccaa gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat
     7621 acagccccat tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa
     7681 aacagatgaa gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg
     7741 gaaaaaagaa gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga
     7801 tcttttgagt atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact
     7861 gcagtcatta tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca
     7921 tgcttttcac aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct
     7981 tcttttattc gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa
     8041 aaatgatcat ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta
     8101 tggcgatgca tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat
     8161 ttgtctaccg cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg
     8221 ctgatttttt tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt
     8281 ctcttctact gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt
     8341 gatttcttgg agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac
     8401 gaaaaaatat tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat
     8461 ttcccgccag ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag
     8521 tgtcaagatg acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc
     8581 gacgatccaa aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc
     8641 caatccaaca tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg
     8701 ttctgctaca tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg
     8761 ataatgcgtt tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt
     8821 tgtaatcttt gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact
     8881 tttaacttcg acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc
     8941 caatgaattc tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa
     9001 acaaacaggg atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc
     9061 ctgataagtg aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt
     9121 tctataatgg tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc
     9181 gcactttctt cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt
     9241 tttccaagag ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag
     9301 tccgcttctg cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct
     9361 gcactttgga caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt
     9421 aaaatatctg actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca
     9481 atcgacaatt ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa
     9541 cattgagcta accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa
     9601 atgctagcca tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg
     9661 ataagagatt gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc
     9721 agctacttgc agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg
     9781 cagaggtgtc ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat
     9841 tttctgtaat tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc
     9901 tgcgtctttg atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa
     9961 ttcatcaatc agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt
    10021 cttgacactt aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc
    10081 gcccatctcc tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt
    10141 taacaacagt tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga
    10201 aatatcacct ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat
    10261 cgttttatct tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt
    10321 acgggaacgt tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag
    10381 caaagcagat ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga
    10441 gcgggttcct gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc
    10501 gcccatctcg cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc
    10561 cccgaacaga gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt
    10621 cgtaccggag tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg
    10681 accatgaatc ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc
    10741 atgtaaagta ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc
    10801 agcaattgct ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac
    10861 agtccctctt aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat
    10921 ccagcaatga taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg
    10981 gtttttaaaa ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa
    11041 attaatcaaa gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg
    11101 ttcatctggc acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac
    11161 aggcaactgg tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt
    11221 ctcagtcatg atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc
    11281 caataatatc tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc
    11341 gatcctagtg atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg
    11401 attgaattgt tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag
    11461 cattcctgtt atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc
    11521 gcaaaatagt tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaaaat
    11581 gtaataggca ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc
    11641 agccattgga tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg
    11701 ctgcttcgta ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag
    11761 gacatcaaga taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg
    11821 ttctgcacct gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg
    11881 ctcacgggtt tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg
    11941 aatacaaagt gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc
    12001 catcttattt gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag
    12061 ctttcaactc aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag
    12121 tggctacaac attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt
    12181 ctacacttgc tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca
    12241 atgtaggaat gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc
    12301 cttcgaagcc gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga
    12361 tctcatccat cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta
    12421 cgcctttaaa ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt
    12481 cccattgtac gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc
    12541 ctccgatcgt atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa
    12601 ctgctggatc aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg
    12661 agatatttcc aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga
    12721 tgatgtcaag ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg
    12781 tctctcttgc agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac
    12841 cgcctaccaa atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt
    12901 tttcaggcac ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta
    12961 ccactaaagt gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa
    13021 cacgatcttt ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga
    13081 gttcgtgatt gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg
    13141 ctgttaattc tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct
    13201 aagtaatctt tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct
    13261 agcaagatga tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat
    13321 aattcatctg gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt
    13381 tctaaagcag ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg
    13441 gctaccattc caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt
    13501 tcaattgcat ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag
    13561 acatcttctt ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca
    13621 tgttccagaa gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct
    13681 gctatagctg ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa
    13741 gtttttagaa cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg
    13801 tttttcgctt tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca
    13861 agtagcgtag tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat
    13921 gctgctagat cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa
    13981 tcattatctg ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca
    14041 cccgccggta cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt
    14101 ttttctgcat atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc
    14161 caaatcgttt gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg
    14221 tatttttggt tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct
    14281 ggaattaatc gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca
    14341 tcatgcgtga gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag
    14401 gatcaggatg gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca
    14461 ttggtggtac aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat
    14521 gagttaattt tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt
    14581 cgtaagtccg aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt
    14641 attctgcagc attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag
    14701 gtttacctgt tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa
    14761 tctgtaggat atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag
    14821 taatcacttt cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt
    14881 ctaaaccttg gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt
    14941 ttgcgccccc ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag
    15001 aacaaggacc agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca
    15061 cgactgtatt gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca
    15121 cagcattttc tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac
    15181 cttttagtcc aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt
    15241 tttcgatatg atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta
    15301 ctatcattgt ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac
    15361 tttttcaagg tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt
    15421 tcgctccttc tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga
    15481 aattgcctct acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat
    15541 agccactata gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat
    15601 catagataca aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa
    15661 tagctaaagt aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc
    15721 aaactgatgt aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt
    15781 atgaaacttt ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt
    15841 tttattgaag aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca
    15901 tgacatatag aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa
    15961 gattgctact gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt
    16021 gactcaacat ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc
    16081 gtaagaaccg ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct
    16141 tctgctttga atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac
    16201 gaaaaatctt cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc
    16261 catttcgttt ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt
    16321 cctgccactt tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct
    16381 gataggcctt catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat
    16441 gcgggtaatt ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc
    16501 tctccagcga tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt
    16561 gtaaaaatga tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga
    16621 gcaatcggca aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga
    16681 tacaatccgg ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta
    16741 tcggtcgttg ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt
    16801 ttattgagcg attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat
    16861 ttgacaccta atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt
    16921 ttatttactg tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc
    16981 atctgcaaaa acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc
    17041 accgccatag cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc
    17101 tttaaaatcc catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta
    17161 agaatccatt ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg
    17221 tttttccgct ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct
    17281 ttttacatct gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac
    17341 aagtcgatcg gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc
    17401 ataagattca tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa
    17461 attcgttttt gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt
    17521 gtaagaaggc tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc
    17581 cgccatcaca tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta
    17641 atgagatgtc cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat
    17701 atcttctata gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt
    17761 gataatcatc cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa
    17821 accgaaggaa tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc
    17881 aagcttatat aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat
    17941 gcgaatcgtc atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg
    18001 atctagtatc gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac
    18061 ttcaagtttg ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa
    18121 atggttttgt cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa
    18181 gtcctgatct tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt
    18241 ttcttcttcc aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata
    18301 cccgataaaa tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat
    18361 cgagatttgt tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac
    18421 cggattgtct ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa
    18481 agggggacgg cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt
    18541 gtcataattt gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc
    18601 aaaatctttt ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg
    18661 ccatgatcca tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc
    18721 ataacaaaca tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc
    18781 taaccatttt atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga
    18841 agcagttctg tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat
    18901 tcggcccccg aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac
    18961 cccatcagat atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac
    19021 acaatagctt catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa
    19081 ggactcgcca aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat
    19141 ataatttggt cttttacttg cttttcttct tcaggagaca gctttgtcaa atccagttca
    19201 taaccaaaaa cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg
    19261 gtctgatgat ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg
    19321 cttgttccat attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac
    19381 tgcggcatat aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc
    19441 cataagattt ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata
    19501 taacgatgcg ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa
    19561 agcgaacggt tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc
    19621 atttgtttct caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat
    19681 ctggaagcag aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac
    19741 agttcagaat caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg
    19801 ctatgagcat aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac
    19861 cagtctccta atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg
    19921 aacatttcga tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg
    19981 aagtcaaagt aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga
    20041 cgacctcttg cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat
    20101 ccctgacttg agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat
    20161 tcccacgaaa aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct
    20221 ttttcaagtt cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca
    20281 gtattttctg tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc
    20341 gttcctcgac ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt
    20401 ctttcattga catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca
    20461 aagtctagct ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt
    20521 atctttacag atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa
    20581 taaatcgctg tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc
    20641 atcacataag tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg
    20701 tcatatctca gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt
    20761 cgaaagtcca tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac
    20821 cggtcaggat ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta
    20881 ttgtctttgt actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct
    20941 tctatcttta tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca
    21001 tcaaataaga tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca
    21061 acaaaacgga aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt
    21121 tgtcaataaa aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg
    21181 tcgcacgaaa ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga
    21241 cgatccatgt ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt
    21301 atgacatcag tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta
    21361 cgatcggcaa agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg
    21421 aagaagaagc tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc
    21481 ctaaacaatg ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga
    21541 aagaatcgat taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg
    21601 acatccagca agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac
    21661 tggaattgtt cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg
    21721 atatggatga attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc
    21781 agcattttat gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga
    21841 cagaaaaaca aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta
    21901 ctgccattct tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa
    21961 aaaatatcca aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg
    22021 caaacctctc ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag
    22081 ctgttcgaat ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt
    22141 atcctgcacg attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga
    22201 gaggttgtga aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc
    22261 tcttacttat acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt
    22321 agcgtgtaat caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag
    22381 gattgcacgg gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg
    22441 aatgagtatg catttccgtc agttatatac gttggcggaa attccttaga aacatggtca
    22501 gccacactag cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac
    22561 cacgcaacgg ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt
    22621 agcttcaaat caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt
    22681 agctgtttga ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc
    22741 aatgcgattt gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat
    22801 ttgtcgtctt tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga
    22861 ttagggtatt ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg
    22921 tctgaaacta attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct
    22981 ccaccatgta cccaaattag aacaggaaca ggccccttgc tgttttttgg atagtagatg
    23041 tcataagtat tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt
    23101 ttttctgctt gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg
    23161 ttgatcaaga atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg
    23221 atcccaagaa tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg
    23281 ctccctcagt agttagtatt aagcattctg attataccat agtgttattt cctatcaagc
    23341 tctcctagat gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa
    23401 attttttcca ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt
    23461 gaggacttaa gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa
    23521 tcacttgctg gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact
    23581 aatttatctt cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat
    23641 tctaaataat aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc
    23701 ccccctgatg ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt
    23761 gaatcatttg agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa
    23821 taacgaaaaa tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact
    23881 caacagtcct tattggcaaa tcgccaaaat aatgtagact aactaaaaag actaagaaac
    23941 aataacaaaa gttaacactt cttagtcttt ctttagcacg tctatttatt aaagatttta
    24001 gggccttttc tcatgttttt acgatcattc ttaggtgttt ccatttgttt ggatcggcct
    24061 ccaccttgtt tctttttgat caattctttc attttttctt tttggtccat ttttattctc
    24121 ctttaacgaa tgaagcgact aattcgatat gatgggtttg cgggaaaaga tctacaggct
    24181 gaactttctt cacggcataa cctgcttctg caaataactt agcatctctt gcaaaagttg
    24241 ccggattaca tgagatatag acgattcgtt ctggtgacat ttcgattgct gattcaataa
    24301 aatgatgatc taatcctttt cgtggagggt caactacgat cacagatggt ttgatccctt
    24361 tttcttgcca ttttttcata actttttctg cttttccgac ttcataatga acattttcga
    24421 ttttattatt taaagcattg aactgtgcat cttcaattgc ttctggaata acttccatcc
    24481 catacacctt atccactctg tctgctaaag acaagccaat cgtacctatt cctgaataag
    24541 cgtcgatcac aatgtctttt ttatctaact cggcaaactc gatggctgtt tggtacagat
    24601 actctgcttg ttgagtattt acttgataaa atgactttgc tgagattcga taggtttttc
    24661 ctaatagctg atcttcgata tagtctctgc catataaaac tttttctttg tctcctaaaa
    24721 tcacatttgt tttttcttca ttgatatttt ggatgaccga aaccacttct ggcaattctt
    24781 catgaatagc ttcagcaatt tccttggatt taaagaattt ttctttacga gtaactaaaa
    24841 cgaccatcat ttcatgggaa taatgaccac gacggataat gatgtgacga agctgtcctt
    24901 catttttttc ttcattatat cctcgtactt ggaaccgttg caagatatct ctgactactt
    24961 tgattgcttg atcgatgaca ggatcttgga tataaaaatc ttcgataggg actagatcat
    25021 ggctattctt tttgtaaaaa ccggtggcta actgtccatc gatacggcga acagggatct
    25081 gtgctttatt acggtagccg aatggttctg ccattccgac cgttggaagt acaggaactt
    25141 ctggcatctt agcaatcttc gccattacat gctctacttg tttttgtttg aagacaagct
    25201 gttgttcata cttcaaatgg gctaaaggtg cgatacctat gcgaagtaat gcattgtttt
    25261 ctacaggctg acgatcaggt gattgtgtaa taaatgtttc gacttttcca tatccaaact
    25321 tattgcctgc ttttactact agtatttcta cttcttctcc agggagtgca ttttcgataa
    25381 ataatggata accatcgatt tttgctaccc ccattccttc ataactcaag tcgacgattt
    25441 cgactgtata tcgttcgttt ttctttacta tttgctgttc tgtcatattt tgctccttta
    25501 agcctatttt tcaatcctac tgaactctac tataaatcag ctaattacac aactaaaacc
    25561 ggcactgacc ttactacgtt cagcaccggc ttttgattaa ttcttttgat taggtgtttc
    25621 gtctgctatt tttccgtcac cgtcgatatc ttcatccgtt aaacgttcga cttcttttac
    25681 gaattctttg ctgacaacct ctaattcttc ttcatctgta cctgtgattg cttcatcagg
    25741 aatctgatcg gtattggcaa agaactcaat gtgttgatga agattttgga aatgcatcgg
    25801 tgcttccccg ccgtattcac catctaagtt gatcattact ggtcgattga tatcatcttc
    25861 tgtttcaacg aacaaactgc ttgttttggt atagattatt cttggatcat cgacatgtcg
    25921 accgccattc agcatgagtg ctgcaatatg caagatctca aagatatttg ctgtcttcac
    25981 aatgattaaa gaaaacttcc catcatccaa tttggcatct ggcgcgatct gttcgaatcc
    26041 tccaacagag ttcgtcagcc ctaggaaaaa catcgaagca tttccgatat attccccttc
    26101 atcatacttc aagcgcattt tgattggctt tactcgaggg agcatctctg ctcctttcgc
    26161 aagataagca agataaccaa aaatactctt tagttctgat ggaacttcat aagtcaattc
    26221 tgtcaaatag ccacctgcag cgatattgat gaagtaactg ttatcagaaa ggccgatgtc
    26281 catttttacg gtttggtgtt tttttatcac ttctgctgct gctttaatat tatctctagg
    26341 aatttttaat gctcgagcat agtcgttcgt tgtaccagct gggatgatgg ccattttagg
    26401 acggtgttca agcggagcga ttccattaac cacttcgttg attgtgccat cgccaccagc
    26461 tgccactact agatcaaaac ctagttctgc gactctacat gcttcattct tggctgaatc
    26521 tggttctggt gtagttgcat aggcgctggc ttcaaatcct gcttcttcta atgcctgtag
    26581 gatatccgcc aaattttttt tcaataattc ttttcccgag gtggggttat aaattacgcg
    26641 tgcttttttc attttcagcc tctatcgttt cgctaattca tctttaagta gcttattcac
    26701 gacaccagga tttgcttgtc ctttagttgc tttcatgatt tgaccgacta ggaagccaac
    26761 cgcacgatct tttccatttt tgaaatcatc gacagattgt tggttgttat ccaaaatttc
    26821 attaatgatt ggcagtagtt tgttcggatc agataattgc acccaaccat ttttttcaac
    26881 tacttcttgg gcttctccgc catttgtgat caattcacgg aagacttttt tggcgatttt
    26941 tgagctgatc gttccatcgg cgatcaaacg aatcattcct gctaagttct caggtgtcaa
    27001 ttttgtctca gctaattcca gtttctcact gttcaagtaa gcagaaactt cacccatcaa
    27061 ccagttagat gcttgcttgg catctgcacc attttccaaa acagcttcaa aaaaatcaga
    27121 catttctttt gtaagtgtca ataccattgc gtcatactct ggaagtccaa actcacggat
    27181 atagcgttcg cgacgagaag ctggcatctc aggcaaacta gcttttactt tttgaatcca
    27241 ttcgtcatcg atcacgaatt tcgggatatc aggctctggg aagtaacgat aatcactaga
    27301 cccttctttg acacgcatca aaactgtttt attcgttgtt tcgtcaaaac ggcgtgtttc
    27361 ttgttggatt tctcctcctg aaagaagaac ttttgcttga cgtttttctt caaaagctaa
    27421 accttttttc acgaaagata aagagttcaa gtttttcaat tctgctttcg taccgaactc
    27481 ttcttgtcca taagggcgaa gagaaatatt ggcatcacag cgcatagagc cttcttccat
    27541 tttcacatcg ctgacttctg taaattggat aattgatcgc aatgcttcta gataagcgta
    27601 tgcttcttct ggtgaacgca tatcagcttc agaaacgatt tcgatcaaag gcgtgccttg
    27661 tcggttcaaa tcaacgtaag aataaccgcc aataccatgc atgttttttc cagcatcttc
    27721 ttctaaatgg acacgttcga tccggatttt tttcttttta ccttctactt cgatttcgat
    27781 ccatccgtca tgaccgattg gttgatcaaa ttgggaaatt tgatatgctt ttggattatc
    27841 tgggtaaaag taatttttcc ggtcaaaatg agtatcttta gagatttcac agtttaatgc
    27901 caaagctgct ttcatcccga attccagcgc agctttattc attactggca gcacgcctgg
    27961 ataaccccag tcgatcacgt ttgtattgct gtttggttct gcaccaaaat gagctggcgc
    28021 tggagagaaa atttttgagt tggtttttaa ctcgacgtgg acttctagtc caatgactgt
    28081 ttcaaaattc attcgtcttt ccctcctaaa atagctggtt gttgtttatg gaattctgtt
    28141 gcttgttcaa acgcatatgc cgcttgatac atcgtttttt catcaaaata attaccgatg
    28201 atctgcaatc caactggtaa tccttctgat aaacctgccg gtacagacat acctggaagt
    28261 ccagccaagt tgacagggat cgttaagatg tcactcatgt acattgtgat tggatcgtta
    28321 atgttctcac cgattccgaa tgcaactgtt ggtgaagatg gcccgatgat caagtcataa
    28381 tctgcaaaga ctttttcaaa atctcgtttg atcaatgtac ggacttgtcc tgcttttttg
    28441 aagtaagcgt cataataacc cgcgcttaag gagaatgtac ctaacatgat acggcgtttt
    28501 acttcttcac cgaatccttc tgaacgtgag ttgacatata catcatctaa tgatttcaca
    28561 ttttcagagc gataaccgta gcggatacca tcgaatcgtt gcaaattgga acttgcttct
    28621 gatgaggcaa tgatataata aacagccaca ccgtattttg aatgaggcaa gctgacttct
    28681 tccacaacag cacctaatga acggaatgtt tctgctgctt tcaatacagc ttcttttacg
    28741 ccaggggcca caccttcgcc taagtattct ttaggtaaag caatcttcat tcctttgatg
    28801 tcgcctgtca gtccctctgc aaaatcagga actgaaacgc ctgaagaagt accgtctttt
    28861 tcgtcataac cgctgatagc tgtcaatgct aatgcattat ctttcaccgt acgggtcatt
    28921 ggtccgattt ggtcaagaga tgaagcaaag gcaatcaaac caaaacgtga gacacggcca
    28981 taagtaggtt tcatccctac gatgccgtta aaagctgctg gttggcggat acttccccca
    29041 gtatcactac ctaaagaaac tggaatttgt ccagctgcaa ctgctgctgc agaaccacca
    29101 gaagagccac caggtacttt tgttgggtcc caagcatttt ttgttttctt gaaataagaa
    29161 gtttctgtac ttccacccat agcaaattca tccatgttta gcttaccaac agggatcata
    29221 tctgcttgat agattttatc catgaccgtt gcatcataaa ttggttcaaa attgtaaagg
    29281 atcttagatg cagcggttgt caagatatct tttgttacga tattatcctt gataccaatt
    29341 ggattaccag caaggacgtt gctttcatca atcccttttt catctaaagc ttttgcctgt
    29401 tccaatgctt tttcttcgtt caatgtaatg aatgcctcga cctcaggctc tgtttctttg
    29461 attcgttcaa atgttgtttt tgtcaaatct tgtacagtga tttctttcga tacaagcata
    29521 tgatgcagtt cttctagaga acgattgtgt aattctgtca ttatgcgcca gcctccccat
    29581 tatcgatgat tgcgggtact ttgataaagc cgtcttctgt ttccggcaca tttttcaata
    29641 attcatcgcg gttccatcca gcttgcgccc gatcttgtcg catcacgttt actgtttcaa
    29701 ccacattcga cgtaaatgga acaccttccg tatctacttc ttccagcatt tcaaccatat
    29761 cgatgatttt acctaattgg tcggtaaaac ttgcaagttc ttcatctgcg aacgcaagtt
    29821 ttgctaattt ggcaacgtgt ttcacttgtt cttcactaat cgccatacta ccccttcttt
    29881 ctaaacgagt taaaaacttt gctctctatt ttaacacaag agtgccgtgt ctgaaattgt
    29941 ttattgaaat ttgtctgaaa aaactcattt ttcattcttc tactgagcga cttttagcca
    30001 atgcatctgc catttgatct tctgtccaaa cttcgattcc taattcttgt gctttggtca
    30061 gcttgctgcc agcatcttct cctgctacca cgatatccgt ttttttcgat acgctaccag
    30121 tcactttccc gccaagattt tcgatcgtct cttttgcttc ttcacgagta aatcgtgtta
    30181 gtttaccagt aaggactact gttttttctt taaaaggaga ctcgacttct tgcaattggg
    30241 catttcgaag ccctttgtat tcgaaattga cgcctgcttg ttttaattca ttcatcaatt
    30301 catggacttc ttcgttactg aaataagtga ctacactgtc ggcaatgata tcaccgatcg
    30361 tatcaagcgc aataatcgac tcatagtcac ttttactcaa tgtttctaga tcaccgaaat
    30421 gttctgctaa aatttttgct gctttcgctc cgacatgtcg gattcctaac ccgaaaatca
    30481 accgctcgac cgaattatct ttgctgttgt cgattgctgt caaaatatta tttgctgatt
    30541 tttcttttat tttgtccaac gtcaataact cttcttctgt caatttgtaa agatcagcaa
    30601 catctgctac tagtttttta tcatacatct gttctagtac gcgcggcccc aacccatcga
    30661 tattcatcgc atttcttgaa acaaagtggt tcaaaccttc tttcatttga gcagggcatt
    30721 taggattgat gcaccgtaag gctacttctt catccaaatg gacaagttca cttccacaaa
    30781 ctggacaatg tgtaggcaat tggtattctt cgctgtcttt aggtcgttta tctaacacga
    30841 cttgcgaaac ttctgggatg atgtctcccg ctttataaat cagaactgta tctttcaagc
    30901 ggatatcctt caacttaata taatcaccgt tatgcaggct ggcacggctt actgtcgttc
    30961 ctgctactcg gactggtgtc atgatagctg ttggcgttac tacccctgtt cgaccgactg
    31021 tccattcgat gttttcgatt agcgtttcaa cttcttctgg cggaaacttg taagctgctg
    31081 cccatctcgg tgctttgata gtaaaaccaa gttgatcttg tagcgagaat tcattcactt
    31141 tgatgacgat tccatcgatt tcatagggca aatccacacg tttgtcatga tattcttcaa
    31201 tgtatgacca cacttcatca atcgaatgac atagacgttt ttcccgattc gtgtggaatc
    31261 caattttttc tagctcttct agcgcatcat actgggtttt tgcttgcata ggaccgaaat
    31321 cagctactgt gtataaaaaa gtgtccagat ttcttttcgc tgtgatttta gaatctaact
    31381 gacgcaaaga accagctgcg gcgttccgag gattcgcaaa gatatctttt ccctctgctt
    31441 ctctgtcttg gttcaattgg acaaatgacc gttttggcat gaaacattcg cctcttactt
    31501 cgatgttcat tggttctttt aatttgattg ggaccgaccg gacagttttt aggttttcag
    31561 tgatgttttc tcctacagtt ccatccccgc gagtcgcacc tctcacgaaa tttccatctt
    31621 cataacgcaa agaaacggaa agtccatcta tttttagttc acagcaatag tcaactacac
    31681 gtcccactgc tttttgtaca cgttgatcaa aagctatcag ctcttctttg ctgaaaacgt
    31741 cgttcaaact gtaaagcgga atatcatgag tgactttttc aaaaccttct aatacttttc
    31801 cgcctactcg ttgagtggga gaatcagacg tgatcaaatc aggatattgt tcctcgattg
    31861 ctactaattc tgcgtattct ttatcataaa catagtcttc cactgttggt ttatcttcta
    31921 cgtagtattc gcgagaccat tggttcagtc ttgtacgaag ttcttctgct cgtttagcag
    31981 cttcgtcaat cgtcatttca tgttccatca tttaccctcc actactaagc tttttcaatt
    32041 ggcgcaaagg cagccaaaag acgttttact ccttgacttg ggaaagccac atctagttct
    32101 agatccttcg acgtcccgct tactcggacg actgttccta gaccccattt tttgtgtttg
    32161 actttatctc caggtttcca gctgttattt tcccctccag tagcactttt agagggaaca
    32221 accgctgtct ctggctgttt atagctatat gctgtttttt tagcgaatac aggcgttttt
    32281 ttaggcgtag gacgcatgcc ttcgatttcc agtaactcct gatcgatttc ttctacaaaa
    32341 cgagaaggtc ggttatattg cgtccgtcca tataatgttc tggagaaagc atttgttaaa
    32401 tacaacgctt cttctgctcg cgtgatcccc acatatgcga gacggcgttc ttcctccagt
    32461 tcgctttctt ccatcaatgc tcgagataaa ggaaaaaccc cctcttctag accaatcagg
    32521 aatacaactg gaaattctaa tcctttggct gcatgcaaag tcatcaatgt cacttgggaa
    32581 gcatcttctt ctaaattgtc aatgtcggaa acaagtgcca gatcatttaa gaagaccgtt
    32641 agtttttctt ctggtgcatc agcgtcttct tcattttgct gttcgaattg tttgtcgaat
    32701 tcttgtgtga ccgttaaaaa ttcctccaag ttttcgatgc gtgcttgtgc ttctaatgta
    32761 ttttggattt ttaagtcttc tagatatccg ctacgatcca aaacctcttt cgtcaattct
    32821 gtcactgtca agtatgggat catctgagtg acttcttgga tcatctctcc aaatgcacct
    32881 aattgctgtc ctgcttttcc accgatattt gccagatcca cattttgcgc tgcttcaagc
    32941 agtggccatt catgaagaga agcaaaactg cgtagttttt cgatagatcc tggtccgatg
    33001 cctcgtttgg gcgaattaac gatacgctca aaactgattg aatcttgagg atttaccagc
    33061 acattcaaat acgctaaaat atctttgatt tctttacgat cgtagaactt gtgccctcca
    33121 accattttat aaggaatatt tgctttcaac agagtttctt ccattacacg agattgtgcg
    33181 ttcgtccggt acaagatggc aaagtcgccg taattccgat gattgcttcg aatctcttct
    33241 tgcatccggt ctacgataaa acgggtttcg tctcgttcat tatctgcacg gtagtaagtg
    33301 atcttgtttc cttctttgtt ttctgtccac aagttttttg gttttcgatt gctattgttt
    33361 tcaatgactt ggtttgctgc gctaaggatg tttttcgtgg agcgatagtt ttgttctagc
    33421 aaaattactg cagcatctgg ataatctttt tcaaaatcca agatgttctg catatctgca
    33481 ccgcgccagc cgtaaatact ttgatctgca tctcccacga cgcacaggtt cctaaatcta
    33541 cctgccaata aattgactag cgtatactgt gcgtgatttg tatcttggta ctcatctaca
    33601 tgaatataat ggaatttgtt ttgataatac gtgagtgaat caggatgttc ttcaaataat
    33661 cgaatcgtat tcatgatcag gtcgtcaaag tccatacatt gattatattg aagctctttt
    33721 tggtaagcag cataacattt tgccgcaatc tcttcgtaat aactgccttg catttttgca
    33781 aaatcctgag gggtctgcag gctgtttttt gcttgagaga ttgttcctaa tatacttcga
    33841 ggatcatatt ttttcggatc aatgttcagt tcttttagta tccgtttcat caaggtcagc
    33901 tgttcagaag aatcgatgat tgtaaagttc cggttatacc cgataaaatc aacatctctt
    33961 cttaaaatac gcacacacat cgagtgaaaa gtcgataccc agacatcttc gcctccagat
    34021 gcgagaatcg cattgacacg ttctttcatt tcttttgcag ccttattggt aaaagtaatc
    34081 gctaggatgt tccaaggatt gacttctttt tcctcaatca aataggcaat gcggtgtgtc
    34141 agcactctcg tctttccact tcctgcacct gccattagca gtaatggtcc gtcagtgtga
    34201 agaacagctt ctttctgacg ggggttcatc ccgtttaata attcagcttt ggatgacatt
    34261 aacaacatcc tttctttta
//
LOCUS       DACENJ010000006        31252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.006, whole genome shotgun sequence.
ACCESSION   DACENJ010000006 DACENJ010000000
VERSION     DACENJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.006"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..253)
                     /locus_tag="IW748_000194"
     CDS             complement(<1..253)
                     /locus_tag="IW748_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAQ1103130.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNAT"
     gene            332..763
                     /locus_tag="IW748_000195"
     CDS             332..763
                     /locus_tag="IW748_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1103131.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(862..1095)
                     /locus_tag="IW748_000196"
     CDS             complement(862..1095)
                     /locus_tag="IW748_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1103132.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            1278..2948
                     /locus_tag="IW748_000197"
     CDS             1278..2948
                     /locus_tag="IW748_000197"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1103133.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            3095..3481
                     /locus_tag="IW748_000198"
     CDS             3095..3481
                     /locus_tag="IW748_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103134.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            3468..4166
                     /locus_tag="IW748_000199"
     CDS             3468..4166
                     /locus_tag="IW748_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1103135.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            4171..4635
                     /locus_tag="IW748_000200"
     CDS             4171..4635
                     /locus_tag="IW748_000200"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1103136.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            4632..5543
                     /locus_tag="IW748_000201"
     CDS             4632..5543
                     /locus_tag="IW748_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1103137.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            5778..6317
                     /gene="pyrR"
                     /locus_tag="IW748_000202"
     CDS             5778..6317
                     /gene="pyrR"
                     /locus_tag="IW748_000202"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1103138.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            6331..7614
                     /locus_tag="IW748_000203"
     CDS             6331..7614
                     /locus_tag="IW748_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1103139.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            7627..8553
                     /locus_tag="IW748_000204"
     CDS             7627..8553
                     /locus_tag="IW748_000204"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1103140.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            8572..9852
                     /locus_tag="IW748_000205"
     CDS             8572..9852
                     /locus_tag="IW748_000205"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1103141.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            9857..10933
                     /gene="carA"
                     /locus_tag="IW748_000206"
     CDS             9857..10933
                     /gene="carA"
                     /locus_tag="IW748_000206"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1103142.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            10938..14123
                     /gene="carB"
                     /locus_tag="IW748_000207"
     CDS             10938..14123
                     /gene="carB"
                     /locus_tag="IW748_000207"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1103143.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            14177..14953
                     /locus_tag="IW748_000208"
     CDS             14177..14953
                     /locus_tag="IW748_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1103144.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            14950..15882
                     /locus_tag="IW748_000209"
     CDS             14950..15882
                     /locus_tag="IW748_000209"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1103145.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            15879..16595
                     /gene="pyrF"
                     /locus_tag="IW748_000210"
     CDS             15879..16595
                     /gene="pyrF"
                     /locus_tag="IW748_000210"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1103146.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            16602..17231
                     /locus_tag="IW748_000211"
     CDS             16602..17231
                     /locus_tag="IW748_000211"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1103147.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            17219..17533
                     /locus_tag="IW748_000212"
     CDS             17219..17533
                     /locus_tag="IW748_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103148.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            17571..18212
                     /locus_tag="IW748_000213"
     CDS             17571..18212
                     /locus_tag="IW748_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1103149.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            18243..18947
                     /locus_tag="IW748_000214"
     CDS             18243..18947
                     /locus_tag="IW748_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1103150.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            18944..19846
                     /locus_tag="IW748_000215"
     CDS             18944..19846
                     /locus_tag="IW748_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1103151.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(20004..21710)
                     /locus_tag="IW748_000216"
     CDS             complement(20004..21710)
                     /locus_tag="IW748_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1103152.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            21900..22523
                     /locus_tag="IW748_000217"
     CDS             21900..22523
                     /locus_tag="IW748_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103153.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            22973..24022
                     /locus_tag="IW748_000218"
     CDS             22973..24022
                     /locus_tag="IW748_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1103154.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            24019..24900
                     /locus_tag="IW748_000219"
     CDS             24019..24900
                     /locus_tag="IW748_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103155.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            24903..25673
                     /locus_tag="IW748_000220"
     CDS             24903..25673
                     /locus_tag="IW748_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1103156.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            25952..26806
                     /gene="aroE"
                     /locus_tag="IW748_000221"
     CDS             25952..26806
                     /gene="aroE"
                     /locus_tag="IW748_000221"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1103157.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            26984..28147
                     /locus_tag="IW748_000222"
     CDS             26984..28147
                     /locus_tag="IW748_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1103158.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            28264..28674
                     /locus_tag="IW748_000223"
     CDS             28264..28674
                     /locus_tag="IW748_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1103159.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            28848..31076
                     /gene="topB"
                     /locus_tag="IW748_000224"
     CDS             28848..31076
                     /gene="topB"
                     /locus_tag="IW748_000224"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1103160.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT        10210 a         5455 c         6913 g         8674 t
ORIGIN      
        1 tcgttgcgtt tttgtcgacc gtagaaacta gtgttttgct gtctgaaaaa cagaaaatcg
       61 tttcattctg taagtcgttt ttctttagaa attctaaagt ttttaaaaag acttcgaact
      121 ctgcttgatg gtttgtccct atagaaagcg ggatagatag ctgttcttgt ttaccttgat
      181 agacaatcaa gattccacct ccactaggac cagggttgcc ttttgtagag gcatcaatgt
      241 atacttttaa cataaaaaga gattctcctt tttgttcaaa ctaatgttat ggtaagatta
      301 tatcattact gaatcggtga gaggttagag aatgaaaaaa aatattcgat ggcagcctga
      361 actggcacat acgatcatct attggtcatt gaccttgatc attctgtttt ttagtctgat
      421 tctttcttta gaaaacacag gtccttattg gaaaagcaat tttgttttag gtatttttct
      481 tgtttttcta tttctaggtt tgcggcgttc ttttatttta aaatcaaact ctgtgattat
      541 acgttatgct gctttttgga aagaccgaga aatatctgtc ggacagattc gatctttgac
      601 gccaactaat actggggtgc aaatgatgtt gcaagatgag aaagccccat ctgttttcat
      661 gatgcgaaaa aaagatcaag tagtcctttt tgatcatttt gaaaaaaacg gacccaaatc
      721 agaatcaatt ccttatcctt atacgaaaaa aagaaaaagc taattttaaa aatcagaacc
      781 taaaaaaacg aatttttaca ggaaaagaaa agagtgagac gtacggttca tcccgtttag
      841 cgtctcactc tatttgttta tctattcatg catactagta tcggtagtct gaacgttgcc
      901 tttcttgaca tgggcagctt gtgttcctcg attcccttcg atgatctcaa attcgaccac
      961 ttgattttct tccaaagttt tgaatccctc ttcttcaatc gcagtaaaat gtacaaaaat
     1021 ttcttctgtg tcatcataac tgataaagcc gtatcctttt ttgttgtcaa accattttac
     1081 aattcctgtg gtcaaagcac tcacgacctt tctaaagtct gatcatagcc ttcattataa
     1141 agagtattta taagtacgtc aaataaatta aaaacctgta attttatgtt ttttttctga
     1201 ataaaaaaag gtataattgg catatcttaa caaagaagat attttgttga ataaagttcg
     1261 tgaaaagggg taatcaaatg aagaatgatc tgcaaatttc acaagaaaca gagctagaac
     1321 cgattgttga agtggctagt aaattagatc ttaaggaaga cgatttggaa ctgtatggga
     1381 aatataaggc gaaaatcaat ttttctggaa tcaatcgatc aaaggataac gctgaggggc
     1441 atttgatctt agttacttcc atcaatccaa caccagctgg cgaaggaaag tcaacgatca
     1501 ctgtagggtt aggagacgcg ctcaataaaa tcaataaaaa atctgttatt gcattgcgag
     1561 aaccctcact tggtcctgtg atgggaatca aaggtggtgc tgcaggcgga ggatatgcac
     1621 aggtattgcc tatggaagat atcaacttgc attttactgg tgacatgcat gctatcacaa
     1681 cagctaataa tgcattatct gctcttttag ataatcatat tcatcaagga aatgaattga
     1741 acatcgatgc ccgccgagtg atttggaaac gggtagtcga tctcaatgat cgtgaactgc
     1801 gtaaagtaat tgttggcttg ggtgggccaa tccaaggtgt tccaagagaa gatgggtttg
     1861 atatcacagt tgccagcgaa atcatggcta tcttgtgtct tgcggcagac cttgaagatt
     1921 taaaggctcg tcttgcaagg atcgttgtcg gctatacatt tgaccgtcag cctgtgactg
     1981 ctggagactt gaaagcagaa ggcgctctgg cattactgct gaaagatgca atcaagccga
     2041 atcttgtaca aacaatttac ggaacaccag cttttgtcca cgggggaccg tttgccaata
     2101 tcgctcatgg ctgcaatagt attttagcta ccaagacagc attgagatta gcagattacg
     2161 ttgtcacaga ggcaggattt ggtgcagatc ttggcggaga gaaattttta gatatcaaag
     2221 tacctaactt gaaaaaagcg ccggatgctg tggtgattgt cgccactgtc cgtgcattga
     2281 aaatgcatgg cggaatgaaa aaagatgaac ttaaaaatga gaatcttgat gctttgaaag
     2341 taggttttgc aaatttaaag cgccatatac gtaatatgga acaatatcag ctacctgtga
     2401 tcgtcgcaat caatgaattc gtaacagata cagacaatga actcacactg ttggagcact
     2461 tgtgtgaaaa tcaggggatt cttgccaaga gagcgagtgt atgggcagat ggagcagaag
     2521 gcggcgttga tttagcaaaa gctgtagtac gattgattga tagaaaagaa gctaattaca
     2581 agccattata ccgcctagaa gaaacgatac aagaaaagac agaaacaatc gtcaagaaaa
     2641 tttacggagg aaatggtgtt gttttcagca aaaaagcgca aaaacaaata gaagagttta
     2701 cgaagaatgg atgggacaaa ctgccgattt gtatggcaaa aacacagtat tcattttctg
     2761 atgatccttc actgctaggt gcaccggaag atttcaccat cacgatccgc gaaatcatcc
     2821 caaaattagg ggcaggattt ttagtagcct tgactggaga tgttatgacg atgcctggtt
     2881 tgcctaaaaa acctgctgca ttgaatatgg acgtgacaaa tgacggagaa gtgttgggat
     2941 tgttttaagc ttgattataa tctaccatcg aaggagctgt attttgacta cagcttcttt
     3001 ttttagacga tagggaatta attttgttta cactaggatc attctttttc tttattgtat
     3061 tttgagctat actaaagcat aataggaggg tatgatgaaa acgacattta taagaaaaac
     3121 cggtttttac ggtatgggta gctcgattca gctaagaatc aacaatgaaa aacgcagtgt
     3181 tctcagtcat gaacaaaaga taacgcttga tctggaagtg ccctttacga tgcaggtcac
     3241 cttttattgg ctaaaaagcc ccgtttatac agtgaaagaa gcaaagcctg cttatgtgat
     3301 cacaatgaat tacttgattc ttcaaatcta tccgttttta tttctagcaa ctgggttggg
     3361 ggccgttttc ctacaaaatt tatttttcag tttttttgca gttattgtgt tgattgcgtt
     3421 ctttttctat ataaaaaacc gggcatattt gatcaaggag gaaaattatg gcaaactctg
     3481 atcttttttt aatcagtttc aatcgaatcg aaaaatggat gagagacgag atgggaaatg
     3541 cacgaaatat gggatttacc gaacttgtca gacgcctagc tcaaagaaaa caattaatga
     3601 tcagaaaata cgaagatgat cttttacagc ttgctcagtt aaggaatgcg atcgtccatg
     3661 atcggatcgc agtcgatttt gtcattgcag agccaaatga ttggacagtc aagcgaatcc
     3721 agctgatcga acaagaactg attcagcctg aaaccgtgct gcctcgattt gcaaaacgtg
     3781 taacaggttt tgaacaagat cttccgttac ttgaactgtt aaagatagta gcagaaaaaa
     3841 ggtattcaca atttccgtta tacaataaag gaaaatttgt agcattgatt actttacgta
     3901 atatcgggtt ttggctagca aaagaaagcc aaaaaggtcc ggttgattta acaaataaac
     3961 gagcgttgga tttgatcatc caaaacggaa agtacacaaa ttatcatttt gttccagcta
     4021 aaacacatat ttacgaggtt gaagcgatgt ttcgtgagca agggactttg gaagccgtac
     4081 ttatcactaa agatgggaat cccgatggta atctgctcgg gatcgtacga cctcgtgata
     4141 tttataaaca aatagaaaag gattgatttt ttgttaatta tttattggat attcagcgct
     4201 ttgctagtag gagtggatca gttggtcaaa tggtggatca ctgataatct agcgcttgga
     4261 gagacgaaaa atcttatacc aggtattttg tcactgaatc atattcgaaa cactggagcc
     4321 gcatggagta tgctagaagg gaagatgtgg ttctttacta tagtgacact cattgcagtc
     4381 gttgtgattc tcacattgat gattaaaaat cgtgaaaaag gtaatcgctg gttcatgatt
     4441 ggattgagtt tgattctagc tggtgcgatt ggcaatttta tcgaccgtat tcgtcttggc
     4501 tatgtagtag atatgttcca gacagatttt atgagctttc cgatttttaa cgtagcagat
     4561 gtgacacttg tatgtggagt gatttgtatg ttaatttata ttattttaga tgaaaaggat
     4621 caaagaaaaa aatgacagaa cttaaaacga cgattcacca tgaatctggt aggatcgata
     4681 aagtattgac taatttattc accgattaca gtcgttcgca aattcagctg tggctgaaaa
     4741 atggagcggt ttctgtcaat ggacagccag tcaaagcaaa ttacaaagta aaacaaggag
     4801 acgagattac tgtcttgatc cccgaaccag aaacattaga tgtggtccca gaaaatatcc
     4861 cgttgacgat cgtttacgaa gatgaagatg tagcggtgat aaacaaacct caaggaatgg
     4921 tcgttcatcc ttcagcaggt catacgcatg gcacaatggt caatgcgctg atgtaccata
     4981 tcaaggatct ttctacgatc aacggcgtta ttcgtccagg tattgttcac cgaatcgaca
     5041 aagatacttc cggactgcta atggttgcca aaaatgacca ggcacacgaa tcattagctc
     5101 agcaattaaa agataagacc tctttgcgca aatatgttgc tttagtccat ggagagattc
     5161 ctcacgaaaa aggaaccatc aatgcgccaa tcggccgttc gaaggaaaac cggaaaatgc
     5221 aggcgataag agaagatggg aagtcggctg tcacacattt tactgtattg gaacgtttcg
     5281 aaggatttac tctggttgaa ttaaaactag aaaccggaag aacacaccag atccgtgtgc
     5341 atatgaagta tatcggttat ccgttagctg gagatccagt atacggaccg aagaaaactt
     5401 tgaaaggcaa tggtcagttt ctccatgcgc aaatgttagg ttttacgcac ccgactacaa
     5461 acgaaaagat gatctttgaa gcacctcttc cagaagtttt tgaaaaaact ttagaaaaat
     5521 taagaaaaaa cattgcattt taatttagat aacggtatag taattaacaa cgaaagcata
     5581 aaacttaata gaattccttt aagtttagtc ccgtgaggct aagaagggtg ggagcaagga
     5641 tacgtatggt gcgccataag tagattgctc attgacatag tatagctatc agtgtatcag
     5701 tatacgctca gtctaaacat tcctcctgtc ctcttagggc aggagttttt ttatgcaaaa
     5761 aagtaggggg aatgaaaatg caagcaaaag aagtagtaga tcaggtaacc atgaagcgag
     5821 cattgactcg aattacttat gagattatcg agagaaacca tagtatccaa gatatcgtat
     5881 tggtaggtat caaaacacga ggaatctaca ttgcatcaag aattgccgaa cgcctgaagc
     5941 agctagaaga tatcgatatt cctgtaggag aactggatat cactttatac cgagatgaca
     6001 aaaaagaaaa tccagaagaa ccggaacttc attcttctga catcccagtt tccttagaag
     6061 gaaaagaagt tattttgatt gatgatgttt tgtatacagg acgaacgatt cgagcagcaa
     6121 tggacgcagt aatggatttt ggcagaccaa gaaaaatttc attagcagtc ctggtcgatc
     6181 gtgggcatcg agaattgcca atccgtgcag attatgttgg gaagaacatt ccaacatctc
     6241 gggcggaaga gattttagta gagatgcaag agcttgatgg acaagatcgc atcatgattt
     6301 tgaaagagga agattagagg agagagaaac atgtcggaga aaaaaggatt tcataatgat
     6361 gacgtggtgt tagatatcca cgatcgccca accccaggac attgggtagg attaagttta
     6421 caacacttgt tcacgatgtt cggagcaacc gtgttagtcc ccatcttagt aggtatcgat
     6481 ccaggtattg cgctagtcag ttcaggacta ggtacattgg tgtacttatt tactactaaa
     6541 ggaaagatac ctgcgtactt aggaagcagc ttcgctttca ttgcagcgat gcagcttttg
     6601 atgaaaacag atggttatcc agcgattgca caaggagcag tcacaactgg attagtctat
     6661 ttgattgttg cgctgatcat caaaaagatc ggttcagcat ggctggataa aatcttgcct
     6721 ccaatcgtcg tcggtccagt catcatggtt atcgggttgg gacttgcggc aaatgcagca
     6781 acaaatgcta tgtataatca gggcacgtat gatttcaagt atattgctgt agcgctgatc
     6841 acattaggat taacaatttt cttcaacatg tggctaagag gttttctcgg attgattccg
     6901 attttactag gtattattag cggatacatc gtagccttgc tgtttggaat cgtggatact
     6961 aagccaatcg tagacgctgc ctggtttgct gttcctaact ttgagattcc ctttgtacaa
     7021 tatgaaccaa agctatatct tggagcaatc acaacaatgg cacccatcgc ttttgtgacg
     7081 atgacagagc atatcggcca tcttatggta ttgaataaac taactaaacg taatttcttt
     7141 gaagaaccgg gtcttcataa gacgttgaca ggtgatggat tagcacaaat cgtggcagcc
     7201 tttgtcggag gtcctcctgt aacaagttac ggtgaaaaca ttggtgttct ggctatcaca
     7261 cgtgtccata gcgtgtttgt gatcggtgga gctgcagtat ttgcagtcat tttaggattc
     7321 gtaggaaaat taagcgcttt gattttaagt atccctggac cagtcatctc aggaatcagt
     7381 tttatcttat ttggagtaat tgccgcaagt ggaatgaaga ttttaatcga gaacaaaatc
     7441 gattttgata aaaagaaaaa tctgttgatt gcttcagtta tcttagtagt aggaattggc
     7501 ggactcgtct ttgaagtagg cacatttaca ttgtcggcaa tggcattagc aactgtactt
     7561 ggcatcattt taaatcttat cttaccagaa acttcacgaa gcgaagaaaa ctaaggagga
     7621 aacattatga tcatcaaatc agaacgtatc agcttaaagc atttattgac cgttgaggca
     7681 cttactgatc aagaagttat gggcttgatc cgcagagggc aggaattcaa gcaaggtgct
     7741 aattggactc cgcaaaagcg ccagtacttt gctacaaacc tctttttcga aaatagtact
     7801 cgcacacata aaagcttcga tgtagcggag aaaaaactag ggctagaagt gattgaattt
     7861 gaagcaagca caagctctgt ccaaaaaggg gaaacactat acgatacagt actgacgatg
     7921 tcagcattag gcgtagacgt agcggttatc cgtcatggtg atgagaacta ttatgacgaa
     7981 ctgatacaaa gtaaaacaat ccagtgttcg attatcaatg gaggagacgg aagcggacag
     8041 catccaaccc aatgcttgct tgatttgatg accatttatg aagaatttgg tcattttgaa
     8101 ggtctgaaag ttgccatcgt aggtgatatc actcactctc gcgtagctaa atccaatatg
     8161 cagatgttaa aacgtctagg cgcacaagta ttcttctcag gtcctagaga atggtacgac
     8221 gatgaattcg aagtgtacgg acattatatg ccattggatg aactactaga tcaagttgat
     8281 gtcatgatga tgcttcgggt gcaacacgaa cgccatgatg gaaaagaaag tttctcaaaa
     8341 gaaggctacc atcaggaaca tgggttaaca gttgagcgtg cgaagaagtt gcaaaaacat
     8401 gcaatcatta tgcacccagc accagttaac cgtgatgtag aattagctga ttcattagtg
     8461 gaaggattac agtcgcggat cgttcagcaa atgagcaatg gagtattcgt cagaatggca
     8521 atcttagaag cagtgctaaa tggaaaagcg taattatagg aggatgaaaa aatgaaaaca
     8581 ctcattaaaa acggtcagat caatacacga aaaaatatga cgacaccagc agaaatttgg
     8641 atagaagatg ggcgaatcaa agcaatcggt acaggattct cagaagccga atttgatgaa
     8701 gtgtttgatg caaagggaca attgatcaca cctggtttag tggacgtcca tgttcatttg
     8761 agagagccag gatttacata caaggagacg atcgaagctg ggactcgctc agctgctcgt
     8821 ggcggtttca ctactgtatg cgcgatgccg aatttaaatc ctgtacctga tacggcagaa
     8881 aagctgagac aagtatatga catcattaga aaagatgcag ttgtgaaggt attgcagtat
     8941 gcgccaatca cagaaaatct gcgaagtgag aaattagttg atcaagaagc actgattgaa
     9001 gaaggtgctt ttgctttcac aaatgacggt gtaggtgtcc aaacagctgg gacgatgtat
     9061 ctggctatga aagaagctgc aaaaaacaat aaagccctag tagcccatac agaagatgaa
     9121 tctttattat ttggcggcgt tatgcacgct ggcaagaagg cagaagaact tggtcttccc
     9181 gggatcttaa gcgtaaccga gtcttctcaa attgctagag atctgttgct agctgaagca
     9241 actggtgttc attaccatgt ttgccatgtt tctactaaag aaagtgtccg tgtaatcaga
     9301 gacgcaaaaa aagcaggaat ccacgtaaca gcagaagttt caccccatca tcttattttg
     9361 atcgacgaag atattcccga agatttcggc ttttggaaaa tgaacccgcc gctaagagga
     9421 cgggaggaca gagaagcttt gatcgaaggc ttgcttgacg gtacgatcga ttgcatcgct
     9481 acagaccatg ccccccatgg attagaagaa aaaagccaaa gtttcatgaa atcaccattt
     9541 ggtatcgtag gaagcgaaac agcgtttcaa ctgatctata ctcattttgt agaaactggt
     9601 cgattcacgt tggaacaagt gatcaattgg ttggcagtta aaccggctga gatttttggt
     9661 ttgaatgcag gaacgctaac ggttggtgcc cccgctgatg tagcagtttt tgatattaca
     9721 caaacttgca cgatcgataa agaagacttt ttatcaaaag gggagaatac accttttatc
     9781 ggctggaaag taaaaggcga aacacaaatg acttttgtca acggaaaact tgtgtggcag
     9841 aagggagaat aaaagtatgg aacgtttgct tatattagaa gatggaacag ttttcgaagg
     9901 aaaggctttt ggtgcagatg ctagtgtctt cggagaaatc gtttttacaa caagtatgac
     9961 tggttatcaa gaaactatca ctgatcaaag ttttaacggt caaatcatta catttaccta
    10021 tcctatggtg ggtaattatg gggtaaatag agatgattat gaatcgattt ctccaacatg
    10081 caaaggagta gtagtaaaag aacatgcacg tgtagcttca aactggcgaa accaaatgac
    10141 gctagattca tttttgaaac aaaaaggaat tcctggaata tcaggtatcg atacgcgcgc
    10201 ccttactcgc aaaatccgtg aagtcggaac gatgaaagct agtatagttg atgctggaga
    10261 ttccttcgaa catgcatttg atcagctgaa agcaaccgtt ctagcaacca atcaagtgca
    10321 gcaagtctca actaacaaac cttatccaag tccaggaaca ggtcggaaag tcattgtcat
    10381 tgacttcggc ttgaaacata gtattttacg agaattgagt aaaagaaact gtaacctcac
    10441 agtacttccc tataacacag atgccgagac gattttagcg ctatcaccag acggagtcat
    10501 gctgacgaat ggacctggtg atccaaaaga tgtgccagaa gcaatcgaaa tgatcaaaaa
    10561 aatccaagga aaagtaccga tttttggtat ttgtctcggt caccagttat ttgctttagc
    10621 caacggtgca gatacctata aaatgaaatt cggacatcga ggattaaatc acccggtacg
    10681 agaagtagcg accggaagaa ttgattttac ttctcaaaat catggctatg cagtcgatga
    10741 agccacgatt gatccagaaa aactcatcgt aactcatgta gaagtaaatg acggaacagt
    10801 agaaggagtc aaacacagag attatcccgc tttcagtgtg cagtttcatc cagatgctgc
    10861 ccctggtccg catgatgcac tacatttatt tgatgaattt atggaaatga tggacgcagg
    10921 aaaggagcag taaacagatg ccgaaacgta cagatatcaa gaaaattatg gtgattggat
    10981 ctggtccgat catcattggt caagccgcag aatttgacta cgcaggaaca caagcctgcc
    11041 ttgccttaaa agaagaagga tatgaagtgg ttttagtaaa ttctaatcct gcgacgatca
    11101 tgacggataa agaagtggca gatcaagtat atatcgaacc gatcacactt gaatttgtat
    11161 caaggattct tcgaaaagag cgtccagatg ctctccttcc aactttaggc ggacagacag
    11221 gcctcaatat ggcaatggag ctggcagcat caggtatttt agaagaattg aacatagaac
    11281 ttctaggaac aaagctttct gcgatcgatc aagcagaaga tcgtgatcta ttcaaacagt
    11341 tgatgaaaga attgaatcag ccaatccccg agtctgaaat cgtcaataca gtagaagaag
    11401 ctgtagattt tgcaaataaa atcggctatc ctgtcatcgt tcgtccagcc tttactttag
    11461 gcggaactgg tggaggtatg tgtgaaaatg aagaagaatt acgtatcgta tcagaaaatg
    11521 gattaagtct ttcccccgct acccaatgtt tgattgaacg aagtattgca ggttttaaag
    11581 aaattgaata tgaagtgatg agagattcgg ctgataatgc aatcgttgtc tgcaatatgg
    11641 aaaattttga cccggtgggt atccatacag gtgattctat cgtatttgcc cctagtcaaa
    11701 cattgtctga ccatgaatat caaatgcttc gtgatgcttc tctttcgatt attcgagcac
    11761 ttaagatcga gggaggctgt aatgtccaac tcgctcttga tccacatagt tttaattatt
    11821 atgtgattga agtcaatccg cgtgtttctc gttcatctgc gttagcaagt aaggcgactg
    11881 gctatccgat cgctaagcta gctgctaaaa tagcgcttgg attaacatta gacgaaatga
    11941 aaaatcctgt tacaggaacg acttttgcag aatttgaacc tgcattagat tatgttgtag
    12001 ctaaaattcc tcgctggccg tttgataagt ttgaaaacgg agaacggctt ttaggcacgc
    12061 agatgaaagc aactggtgaa gtgatggcta tcggtcggaa tatcgaagag tctttattga
    12121 aagctgtgcg ttctttagaa atcggtacac accatatcga acttcctgag ttggcagaca
    12181 tcacggacga tgacctaatc caaaaagtgg ttcgagcaca agatgatcgc ctgttttatg
    12241 tagctgaagc tattagaaga gggtacacca tagaagaatt agcggatttg acaaaaatcg
    12301 atttattctt tttagataaa ctgttgcata tcgtagaaat cgaacagcag ttgaaacagc
    12361 agccgttgga tgacaagata ttgaaaacag cgaaacaaaa cggttttaca gacaaaaaag
    12421 tggcggagct gtgggaaata aacgaagaag ccgttcgcca gatgagaaaa gaaaatgatt
    12481 tgattcctgt atataagatg gttgacactt gtgcagcgga attcgaatca aaaacacctt
    12541 atttttatag ttcctatgaa tatgaaaatg aaagttcacg atcagaaaag ccctcaattc
    12601 tagtactggg atctggtcct atccgtatcg gacaaggggt tgaatttgac tatgcgactg
    12661 tgcattctgt caaagcaatc cagcaggcag gatacgaagc aatcatcatg aacagcaatc
    12721 ctgaaacagt ttcgactgac ttttctattt cggataaatt atattttgag ccattgacct
    12781 tcgaagatgt gatgaatgtg atcgagctag aacagccgat aggagtaatc gttcaatttg
    12841 gtggtcagac agcaatcaac ttagccgaac ctttgactgc tgctggtgta aaaatcttag
    12901 ggacaacgat cgaagacttg gatcgtgcgg aaaatcgtga tttattcgag caagcgctga
    12961 aagagttgga tattcctcaa cctttagggg atactgctac aagtaaagaa gaagccgtgg
    13021 ttatcgcttc gagaattggt tatccagttc ttgttcgtcc aagctatgta ttaggcggac
    13081 gtgcgatgga aatcgttgaa aaccagcgag atttagaaga ttacatggaa catgctgtga
    13141 aagcatctcc agaacatcca gtactagtgg accgttactt gcttggtggt gaatgcgaag
    13201 tggatgcgat ttgtgacggt gaaacagtac tcatcccagg gatcatggag catattgagc
    13261 gtgctggtgt ccattcaggg gattcaatgg ctgtgtatcc acctcaaaca ttatctgaat
    13321 cgatcaagaa aacgatcgaa gattacacga tccgcttagc attaggtttg aattgtgtcg
    13381 ggatgatgaa tatccagttt gtcatccatg aaaatgaggt ttatgtgatc gaagtcaatc
    13441 ctcgagcaag tcggactgtt ccattcttaa gtaaggtgac aggaattcca atggcacaaa
    13501 ttgcgacgaa agctattttg ggagagaaac taaagacact tggttatgaa aacggacttt
    13561 atccagaaac aaaagaagtt catgttaaag cacctgtctt ttcttttacg aaattgcaga
    13621 aagttgatac ttacttaggg cctgaaatga aatcgactgg agaagtaatg ggatctgaca
    13681 gacaattaga caaagctttg tacaaggcat tcgaagcttc tggattacac ttgcctgaat
    13741 atggagccgt attatttacg attgcggacg aaacaaaaga agaagcacta gaattagcta
    13801 aacgttttac agaaattggc tatagtctga taggaacgaa acatacagct gcttatttcg
    13861 aaaaaaatgg tctggtcgtt gaaacagtag cgaaaatttc tgaggaagca gcagaaaaaa
    13921 atgtagtcga cttaataaga gatggtaaaa cacaagtggt tgtcaatacg attgataaag
    13981 atcggggaaa tgcttctaaa gacggtttca tcatccgaag agaagcagtt gaacatggta
    14041 ttccattgtt tacttcttta gacacagcag atgcgatcgt tcgagtgatg gaatcccgtg
    14101 ctttttcaac taaccctatt taacggcgag cacatgttaa gacaaaaaga cgaaaaaccg
    14161 ttgcaggagg ggacagatga aacaagaaat gatggaaatc gttcagcaaa aacagctggc
    14221 accaaagatt tttgaaatga cattgaaagg tgatctagta aaagaaatgc agcaaccagg
    14281 acagttcttg catattctcg tgccgcgtgc agacttgcta ttacgccgac cgattagctt
    14341 gaatcatatc gaccacgaaa caaatacttg ccgtatcatt tatcgggtgg aaggtgaggg
    14401 gactagagtc ttctctactc tttctgccgg agaccattta gatgtcatgg gtcctttagg
    14461 caatggtttt gatttgtcca atcttcatac aggagacgaa gtatttataa tcggtggagg
    14521 aatcggtgtc cctcctctat atgagttatc aaaacagtta gtcgaaaaag gaattaagcc
    14581 aacgcatttt ttaggttttg ccacacatga agtcgtttat tatgaaaaag aatttaatcg
    14641 tctaggagaa acaagaatcg ctactgacga tggaacatac ggtatgcacg gaaatgtcgg
    14701 taatttattg ttaggaacga agacggaacc tacagctgtt tttgcatgtg gttcaaacgg
    14761 gttattgaag acagtggaac agctgttttc ttcgcatcaa aatgtccagt tatcattaga
    14821 atcccgcatg gcatgtggga taggggcttg ctatgcttgc gtttgtcata cgccggaaga
    14881 tgaaacagga acaaaaagtg tgaaagtttg tgacgaagga ccgattttta aaataggaga
    14941 ggtaattata tgaatgagaa tcctttagcg gtcaaacttc ctggcctaga attaaaaaat
    15001 cctatcatgc cggcaagcgg ctgtttcggt tttggcaaag aatacggaaa atactatgat
    15061 ttgaatcagc ttggatcgat catggtcaag gcaacgactc caaatgcccg ctttggcaat
    15121 cccacgccaa gagtggcgga aacaccatct ggcatgttga atgcaattgg tttgcaaaat
    15181 cctggtctag acgttgttat gcataaactt cttccagaat tagaggagta caatgagtta
    15241 cccatcatcg caaatgttgc tggtgcttgt gaagaagact atgtagaagt ttgtagcaaa
    15301 attggcgaag caccaaatgt gaaagcaatc gagctgaaca tttcttgccc aaatgtcaaa
    15361 catggcggaa ttgcttttgg aacagattct gatgttgctt ttcaattaac acaagcagta
    15421 aagaaagttt cttctgttcc tgtctatgtc aaattgtccc caaatgtaac agacatcgtt
    15481 ccaatcgctc aggcaatcga agctggaggg gcagatgggt ttacgatgat caatactctt
    15541 cttggtatgc ggattgacct taagacaaga aaaccgattt tagccaatca aacaggtgga
    15601 ctctccggac cagcaatcaa gcctgtcgct attcgtttga ttcaccaagt agcaagtata
    15661 tccagccttc caatcattgg gatgggcggc gtgcagacag tggatgatgt tcttgagatg
    15721 tttatggctg gagcaagtgc tgtagctata gggaccgcga atttcaccga tccgtatatc
    15781 tgtcctaaac taattaaaga gctccctgtg cgaatgagcg aactaggcat cgaatcgctg
    15841 gaaagattaa tcaaagaagt gagagaggaa cgtgaacgat gaatcaacta gaccaacgac
    15901 caattattgc attagatttt tcaactaggc aagaggtaga agactttctt cgatttttcc
    15961 cgaaagaaga aaaattgttt gtgaaaatcg gaatggaatt gttctaccaa gaaggacctg
    16021 aaatcgtacg ttatctaaaa gaggctggtc ataatgtatt tttagatctg aaacttcacg
    16081 atattccgaa tacggtcgaa aaagcaatgc ggggattggc aaagcttggt gttgatctca
    16141 cctgtgttca tgccgctggc ggtattcgta tgatggaggc ggcaatgcga ggattagaag
    16201 aggggactcc agaaggtgga aaacgtccgc tacttctagc tatcactcag cttacttcta
    16261 caagtgaaga agaaatgcat gccgatcaac taatcgaagt ttctttagaa aaaagtgtga
    16321 tccattatgc tagctgtgcc aaaaaagcag gattagatgg tgtggtttct tctgcttggg
    16381 aagtagaagc gatcaaagaa atggcaggag acgaatttgt ttgcttaact ccgggtataa
    16441 gaccagaagg aacagtcgca ggagatcaga caagagtagt cacaccttct caggcaaaaa
    16501 agatcggctc aacttttatc gttataggtc gaccaatcac tcagtcaact aatccttata
    16561 aagcatatca aacgattcaa acagaatgga gctaacctaa aatgaatgtt gaacaaagta
    16621 tcgcaaaaga cttattagaa attgaagcag tatttttaaa tccaagtaat ccttttacat
    16681 gggcaagtgg aatcaaaagc ccaatttact gtgataaccg aatcacgatg agctatccaa
    16741 aagtccgaaa agagatcgca aaaggattag caagcaaaat caaagaagct taccctgaag
    16801 tacaagtgat tgctggaact gcaacagcag gaatccctca tgccgcatgg gtagcggaaa
    16861 tactggattt gccaatggtc tatattcgaa gtaaggcgaa agatcatggg aaaggtaacc
    16921 aaatcgaagg acgtatattt gaaggtcaga agatggttgt gatcgaggat ttgatttcta
    16981 caggcggaag tgtcttggaa gcagctgaag cagcaaaaag agaaggtgcg gatatccttg
    17041 gtgttgcggc tatctttacg tatgagttgc caaaaggaaa agcaaatttc gaaaaagccg
    17101 aaatccctct tatgacgttg accaattatt ctgtattgat tgaagcagct ttagaagatc
    17161 gttatatcga tgagcaagaa ttaactctgc taaaagaatg gaaaaaagat cctgaaaatt
    17221 ggcagattta agcgcgcttg atgaagatag agaaatccga tttgtgtctc cagaatcatc
    17281 aattggaata gcaaaaatcc aaaaactgat tgcgagaagc atcttttcat caggtatgaa
    17341 aagaaacttc tatcgtgatc agtttttgat ttttttaaaa ttcatttttc taagactggt
    17401 tagcagaaag aagagaataa tctcttctct tttttctatt atatatagat tttctctatt
    17461 ttttggtaag ggagctgttt caaaaatggt aaagagcgga aatgttgcga atatcagtta
    17521 tttaatgata tgatattcgg aaaaagaaat ctcaaaggga ggtaactttt atggggttga
    17581 atgaatttat tttaggattg aacaatttag agacgatcac aagggcacct ggatttttta
    17641 aatatacaga gcatacagtt gcagcacatt cgtatcgtgt agcatctatt gcgcaagttc
    17701 taggagacat tgaagaagcg aatggacaac cgatcgattg gaaactgctc tatgaaaaat
    17761 cattgaatca tgactacaca gagcgtttta ttggtgatat caaaacgccg gtgaaatacg
    17821 caagcaaaga gttaagaggg atgctgcaaa cagttgaaga aaagatgacg gacgaattca
    17881 tcaaccaaga gattccagct gagttccaag aagtttatcg ccgtcggttg ttcgaaggaa
    17941 aagatgaaac cgtagaagga gaactattag ccatttcaga taaggttgac cttctttatg
    18001 aatcttttga tgaaatcatc aaaaataacc cggaaaaagt ttataaggag atgttcattg
    18061 aggcagtgac tacgattaaa agctttgaac atcggccttc tgttcagtat ttctttaaag
    18121 agatatttcc ggaactattg aaccaggaat tttatggaaa agaggcgttt ttagaaaccg
    18181 tctcaacgat tgtaaagaaa gaagaaaaat aaaaatgata tgagatgcag ggggaagtat
    18241 agttgaaaaa gaatatggat gtcgaatggg gctattctgg agacaaaggt ccggaaaatt
    18301 gggcgagttt atgtgattgg tttgcgagag gtgcggaatt tccattgcag tcgcctattc
    18361 atttgaccaa gggggaagaa accaaaatag aaggagacac gctttccttt cattatcaaa
    18421 agcaacggtt tactgacaag gaattcaaaa acacgattca tttagtacct tttgaccaac
    18481 tcagttatgt agagttcaag aaagaacgct attatttgac agatattcat ttccacttgc
    18541 cttctgaaca tatcatcaac ggaaaacagg aagatattga atttcatttt gttcatatga
    18601 acagcaaaaa agagaatcta gttgtgggtg tcatgtacca gctaatggta caggaaggtt
    18661 ggctgtataa tcaagaaaat ggggaaagtt ggaacttaga aaaacatgag cattgggtaa
    18721 atccggatgt gttctttccg gagcaaaaaa gccatttcca ttatattggc tcattgacaa
    18781 ctccgcctac cagcgggcca atccaatggt ttgtaatgga tcatgtagga aaactgaacc
    18841 aagagtttct tctccccttc gatggtcagt atgctcgacc gaacaaccga ccaatccagc
    18901 ccttgaatgg aagagaaatt tattatttcg aggagtttga gcaatgaata ttcagcagat
    18961 gaaatacgtg gtcgctgtag caaacaatgg tagtttcaga gaagcggcaa agaaaatgtt
    19021 tgtctcccag ccaagtcttt cagctggtat caaagaatta gaaaccgagt tagggatcac
    19081 gctgtttact cgaacgaatc gaggggcata tttaacagaa gaagggcaag acttcttaaa
    19141 tcgtgccgag aaaatattgg tccagcttga tacattagaa actcactatc taacgagtga
    19201 aaagagagac aatttttcga tcgctgccca gcattatgat ttcttaggtc ctttgaccgc
    19261 acagctactc gaggaattca aagacgagat caaacaattt cggatcatgg agacgacaac
    19321 agcgagagtg atcgaagaag tcaaagagca gcacagtgag atcggcatta tttatatgaa
    19381 tgagcataat caagctggta tcagaagata tttacaacaa ggagagctaa gtggccgcag
    19441 cattggaaaa ttcaaaacgc atattttttt aaggcaagga catccattag ctgaacacgc
    19501 agagatcacc aaagaagaat tgttatctta tcctcaagtt cgttttacac aggatggaaa
    19561 taatttcccg tatttttatg aagaccttat cgagatccct gatcaagaaa cagtgattta
    19621 tacgagtgat cgaggtactt tgatgaatat cgttcttgaa actgatgctt acgcttcagg
    19681 atctggtatt gtgattggag ggattcgcga gcacttgcgg ttaatccctt tggcagaagg
    19741 cgaactaaat gaattttata tcatttattc ggcgaaacgt ccgctcagtg aaatcgctca
    19801 aagatttatc aaagagctta ctcggttact ggaccagcag agctaagaaa agcagatgag
    19861 aacgctgttt attgaacacc gttgatcaaa aaagaggggt tgtgacagaa ttttgtcaca
    19921 actcctcttt ttgtagcaaa caatttataa ctaattgctg ttgtgggaaa gtacgcaaat
    19981 atgattctag aaacactcat tgattacctg ttttgtttca tagctgtaat tttttcttct
    20041 tctggtgtca cgataatcgt cttttggttt tcataaataa cataaccagg cttggcgcca
    20101 tttggttttc ggatatgctt tacctgtaca agatcaaccg gcacttgggc agagtatcgg
    20161 tattttgaaa aataggcagc taactcggca gcttctgtaa ttgtttcatc tgagggcttg
    20221 tcacttttaa tgatgacgtg cgaaccgggt atattttttg catgaagcca atagtcggtt
    20281 tttttagctg tcttcattgt cagctggtca ttttgaagat tgtttttacc aacaagaatg
    20341 agcgttccgt cagaagaaaa gtattgatca ggctgagatg gtttcttacg tttttgtttt
    20401 tttaagttct tctttttcag gtatccttct gaagtcaatt cttctttaat tgcttcgata
    20461 tccattgggc cggcaatttc cagttgagat aagacagatt caagatattg gatttcatct
    20521 ttggcttctt ggatttgttc gccaatcagc ttaacagcgt tcttcagctt ttggtatcga
    20581 tggaaatatt tctgtgcatt ttgatttggt gtcaatgccg gatctagtgc tattttgatt
    20641 ggccgatctt cttcataata attaggtaaa acaacttctt ttgcgccgcg tggtacttgt
    20701 gtcagaaaag tagtaagtag ttcgccattt ctacggtaat tctcagcgtt ttcggattct
    20761 ttgagtgttt gttcacgttt tttcaattta tttttatttc gttttagttc attttcgatt
    20821 tttcgaatca gttctccgcc ttgttgtttt gcccgatctt tctctgcttt ttcgtgataa
    20881 tagctatcta ataattgact gagtgttgga taagctgtta cagcagatgc ctgttcggac
    20941 aatacctgat aagcaatcgg tgtgaaaaaa tctttatttt ccgtttcgta aaaagtagga
    21001 ataggctgat ggctgatggc tgagaaaaat tcgtcccata caaccatctt ttcattagga
    21061 cgttccagta atcttttggt cagctcttga gcgatatcga aaccgattcc ctgaaactga
    21121 cgttggattg cttttggatc tagttcagtt tgggaaagcc gctgaaagat tttttctttc
    21181 tcggagctaa atgggttcaa gacctcttgt tttggcggag caacatattc tacaccagga
    21241 agcaaagaac gataagtgtt ttgagagctt ccaatgtgtt tgatcgcatc cagtattttt
    21301 ccagtttctc gattaactaa gacgatcgta ctatgacgtc ccattaattc aacgatcaat
    21361 atgatatttt gtagatcacc caactcgttt cgtttagcaa agtggaaatg gatcactcga
    21421 tcattttcga tttgttcgat gtcttctaaa atagcgccat ccaaatattt tcgaagcatc
    21481 atcacgaagt taggcggatt atcaggattc tgataatcga tttgggtaat ctgtacacga
    21541 gcataacttg ggtgcgcaga taataataat cgatggtttt tgccgcgtga acgaatcacc
    21601 agcacgattt cattttcata aggctgatgg attttcgaga tccggccaga tagtaacgtt
    21661 tcacgcaatt cgttgaccat cgcgtgggta aatacaccat caaaggacat ggttcattcc
    21721 tcatttcttt tcataagtat attcattata acgaaaaaaa gaagaaaaac caatttggcc
    21781 gaatagtaaa gcaaaagtct agaaaaatct tggactaatt acataaaaaa cagtgtttct
    21841 atcgttgaaa tccaaatttt taggtataat aaaaaatagg agaaaaagta gaaaagaagg
    21901 tgtttgttat ttgggaagta gttaaatcga gagtacaaga aatgaatgaa catttctcag
    21961 tgtcattcgc agaaaatcca agtaagtatt ttcgacgtct tgtttttctg ctcatcttat
    22021 cattgctgtt aacagttgca atgtttttat tgccggaaaa aatccgagaa gagcaagtaa
    22081 atagtctcgt ttattcgtcc ataccaacca gcaaattgaa accttttgat tacagcaaag
    22141 ctgatcagaa aatcaaacaa agccgggcga tatcggtgat gttcagcaaa ccgaacggga
    22201 aaacctatca ggacatgctt gatgttttta atgatcctga gcttatggaa gaattgaatc
    22261 gaccgatctt ttattatccg atcgtttatg atgtacatga actggaacaa aaatataaca
    22321 tacgaacgga tgaagtgaca tttattttct ttgatggagg aaaagaagca aatcggataa
    22381 ctgcgggtaa gggaggaata actgattttg ataaggagtt gatccctgaa ctgaatcgac
    22441 ttccgttagc caatatcaaa caattagaag aagaattgga tagaacggga gtaacttccc
    22501 aaaattccga gattgctaat tgagacattg gcgcgatgaa acggtgggtc tatatagcgc
    22561 tatttttaat tatcagaaca agtgatgttt gatcttcaaa ataacaaata aaaaattaaa
    22621 aaatatagtt gacgaaattc aaaaattcga tttgacttaa atcaattctt aaggaaaggg
    22681 tgacctgtaa tgaacttatc aaaacgtatt tcagcagaaa acaaaaaaaa ttggcgcagc
    22741 tggcgtagat aagttgtatt aatggtgaga ctttcccata gatacaactt tttagagtgc
    22801 atttctaaag cttgtatcta tgctagacat gaacattgac tgccgcatag attagaatct
    22861 atgcggtttt ttgtgtaaag acaatgaccg ctttggccat tgtctttttt tgtagactgg
    22921 atacttgcct gattataata aataaaaaga ttgactttgc aggaggaaaa aaatgaaaaa
    22981 caaacgatta ataacagttg tagtaattat tttagtttat ttagcaggtg catttattta
    23041 tggaaaagcc tcaggcgaaa aagaaagtac ttcgacgaac cagacaaaaa aaacaacagt
    23101 tgttggggta ttgcaatacg tgagccatcc ggcattagat gagatttatc gaggaatcaa
    23161 agatggtctg aaagaagaag gattaaaaga aggagaaaat cttgagatca aatttcaaaa
    23221 tggacaagcc gatcagagca agctagccac aatgagtcaa cagctagtac aatctgatcc
    23281 agatgccttg attggaatcg caacacctgc cgctcaatca ttagcaaaca cgacttcatc
    23341 gatcccagtt gtgttaggtg ctgtaacgga tcctgtgggg gctggattag tcgcttcttt
    23401 agagaaacct ggtggtaatg taacaggtgt ttctgatcag ccgcctgtta cttcacaaat
    23461 caagcttgct tctgatttgt tgccaaatgc aaaaaaagtc ggaatcttgt atgcttcttc
    23521 agaggataat tcaaaatacc aagtagcaca agcagaagaa gctgcaaaaa aagaagggtt
    23581 agaatcagta aaatatgcgg tgccttccac taatgagatt gcacaaacgg ttcaagtgat
    23641 gagccgacaa gtcgatttta tctatgttcc attagataat accattgcca atgcgatgca
    23701 aacagtggta aaagaagcaa acaaagcaaa tatccccgtc attccatctg tggatacaat
    23761 ggtggaacaa ggtggactag caacaatcgg gatcaatcaa tatcaattag ggcttcaaag
    23821 cggtaagatg gcggcagaac ttgcgagtgg aaaagaaaaa ccggaaacga cgcctgttta
    23881 tacatttgat caaggggata cagtgatcaa tcaatctcaa gctgatcgct taggaatcat
    23941 tgttccccaa tcgatgaagg aaaaagccaa aatcattaca gatgactcgc agcaagaaac
    24001 atctaaggag gatgacaaat gatcgtatca gctatttcac aaggcatgct ttgggcaata
    24061 ttaggattag ggattttcat gacttatcgg atcttagact ttcctgatat gacgacagaa
    24121 gggtccttcc cattaggtgc tgctgtctgt gtgacagcta ttactcatgg ggtagcgccg
    24181 attgtagcta cattattagg cgtaggtgct ggcatgatag caggactcgt tacggggtta
    24241 ctttatacaa aaggaaagat accggttatt ttagcaggga ttttagtgat gtcaggattg
    24301 aactcagtta ttttgtttgt gatgcgaaca ccaaatcttt ctttattgaa tcatccgatt
    24361 ctacaagaca gcttccatcg ctttgggtta cctgattact ttgacacagt ccttcttggc
    24421 ttcatctctt tgggcatctt gattgctgta ctgctattct tcttcaatac agatttgggt
    24481 caaggctata ttgcgacagg agacaacgaa acgatggctc ggtcgttagg gataaaaacc
    24541 gatcgtatga aaattttagg tttaactttg tcgaacggtg tcattgcatt atccggtgcg
    24601 ctgattgccc agaacgacgg atatgcagat gtaaataaag ggattggcgt aatcgtcatc
    24661 ggtcttgctt ccatcatcat cggtgaagtg atcttccaag aattgacgct agcagaaaga
    24721 ttgattgcta ttgttgtcgg cagtatcatt tatcagctat tgattttagt agtaatcaaa
    24781 ctagggtttg acacgactta tctgaagttg ttctccgcta tcatcttagc tatttgtctg
    24841 atgatcccgc aattgaagaa agcactgaac ttaaaaacgg gttttgagaa ggaggcatag
    24901 taatgactga tatattgaca atcaaaaatg gcagaaaagt cgtaaacaat ggaatgaatg
    24961 aagagaaagt actattggat gaaattaacc ttacaatcag agaaggggac ttcatcactg
    25021 tccttggcgg gaatggtgct ggaaaaagca cactgttcaa tacgatcgct ggtacgcttc
    25081 aactcagtca aggatcgatc tcttttaaag atcagctgat cacaaaagat tcagaagaaa
    25141 aaagagcacg attcctttct cgagttttcc aagaccccaa gatgggaacg gctcctcgga
    25201 tgacagtagc agaaaatttg ctacttgcac aaaaaagagg tcaaaaacgg aaattccgtt
    25261 tacgtaactt gaaaaatcaa aaagaagatt ttttcagact atgtcaagaa gtcggtaatg
    25321 gcctagagca acacttggat actccagcgg gtgaattatc tggtggtcaa cgccaagcat
    25381 taagcttgct gatggcgacg atcacagctc ctgagttact attattagat gaacatactg
    25441 cagctttaga tcctaaaaca gctaaagcat taatgcgatt aacaaatcag cggatcaatg
    25501 aacaaaaatt gacttgtctc atgatcacgc atcgaatgga agacgccttg aaatacggaa
    25561 atcgtctgat cgtactccaa aaagggaaaa tcgttcaaga tatttctgaa aaagaaaagc
    25621 aacatttgac aatgggcgac cttttgcagt tctttgaaga agtagtggat tgatttcgac
    25681 agaaaaattc ctttcagtag aaggacttga aaactcattg acatcttcct ttgattcatc
    25741 ctatacttaa aacaataaac aattaaacat atttgggtgt tttgtaggat cacgtagtca
    25801 atgattttaa caattaaata gaatattctt tgctctgaca agtaaacgat gagaaaagaa
    25861 tttgctactt tgccacctag acgtttcgta ctttttcgta tcaaacgcta agtggctttt
    25921 tttattgaag taagaacaaa ggaggaataa gatgatttct ggacaaacca aattagctgg
    25981 gctctttgct gatccggctt ctcacagttt atccccattg atgcacaata ctgcttttga
    26041 agcaaacaac atcgatgcgg tgtatttagc tttccaagta aatccaatga atttgaagca
    26101 agcagtcgaa agtattcgaa cttttgatat gttaggtgta aatctttcga tgccgaataa
    26161 aacagcggtt attccttatc tggatgaggt aagccaggaa gcgcaattga tcggagcagt
    26221 gaatacgatc gtgcacgaaa agggtcgttt gattggctat aatactgatg gtgcgggctt
    26281 catgcaatcc gtgcaagaag ctgggatttc gatcaaaaag aaaaaaatga ctctactggg
    26341 agctggaggt gcagcaaagg cagtcactgt tcaagcagcg ttagacggcg ctgaggaaat
    26401 cgtagtttat aaacgaaaca accaaacatt cgctcaagta aaagaaacat tcgaacaact
    26461 cagctatcga acttcttgta agataaaagt tcgtgacttt gccgatacgc atcaattaag
    26521 aaaagacttg aaagaaagct gtcttctagt caatgcaaca gatatcggga tgggaaagaa
    26581 aatcaatcag tcaccgctct tagatataag catgatgcat gagaatctag cggtttttga
    26641 tttgatttat gcacctagag aaacacgttt attaaaagaa gcaaaaaaag taggagcaaa
    26701 aggatacaat ggacttggca tgttgatcta ccaaggagca atcgcgtttg aattatggac
    26761 aaaggaaaag atgccgatcc atgtgattga aaagctgttt gattaaaaaa ggataataga
    26821 taaagatctt ttattctcgc ataaacggtc tcaagaaaag taatccttta tgtatcgaag
    26881 catttatttc gttatttttt attaaattat caaaaaatga taggcttttg tcgattggtt
    26941 atcgtatcct ttatttatca gtaagaaacg aggaggaacc acaatgagtc ggatggatcg
    27001 ctataaggac atccataaga aggcgaaacc tctgaagcgg gatacgggga gtatttttgc
    27061 tagaagagaa cgaaaacgaa cgcgtatgga aaatgaggaa gatgctaccc gtatttatga
    27121 aagagagcag cattcatttg aacatttgaa ccaatacgag caacagatat acagagatgc
    27181 accaagagca gaagaacaac aaaaaaataa aaaatttttc aatcggcaaa agaagaacaa
    27241 accgttaaaa cctcgaagaa agcgatcgtg gaaaaaaatc attctatcga ttttgcttgt
    27301 tttgatcctt tattcaggta tctcattttt tattggtaaa tatgtagctg aacatgacac
    27361 aagcttagag gcagcgactt cagaaacctt tcatgggcaa aaagcgacga atggtgcgca
    27421 taatattttg atccttggaa gtgatacacg cggagaagat gccgggcgag ccgatacgat
    27481 catggtctta cagttggatg gtccttccca taagccgaaa ctgatttctt ttatgcgtga
    27541 cagcttcgtt acgatcccag gggtaggaca aaataagatc aattcagctt atgcatacgg
    27601 aggagccgat ctagtgcgtc aaactttagt agaaaatttt ggaatcgatt gtcaatacta
    27661 tgcgaaagtc gatttcaaat cgtttgaaaa agtgatcgat gccttattca tgaacggagt
    27721 gaagatcgat gcagaaaaag atctgaatct ggatggtgta gatattaaaa aagggggcca
    27781 gaagatggat ggacatgttc ttttgcagta cgcacgtttc cgaatggatg aacaaggaga
    27841 ctttggccgt gtcagacgcc aacagcaagt aatgaacgct attttcagcc aattgaagaa
    27901 tccattgaat ctgatccgtg caccttatgc agctggtaaa gctttaggat atacatccac
    27961 agatgtcact gcttggttct tgacgaaaaa cctcttttcg attgcacgag gagctggtgg
    28021 cattgatcga ataagtgtgc cagtagacgg ttcgtggaat tatgggcaaa gtgattatgc
    28081 tggcagtatt cttgaaattg ataatcaaat gaatcaatct gctattacag attttttgaa
    28141 taaatgatca taaaaaagat caagaagaag ctgcgcaagg cttttgtcat gaatcaatca
    28201 aacgattcac tcgtaaaaaa cgctttctta tggtagattt aaccaagaga ggagctgttt
    28261 acattggata tctatctgaa cttcaaaaat gagtcgcgtg aagccatcgc tttttatgaa
    28321 aaagtttttc agactgaagc atcaggtatc accacatacg gtgaaatgcc tggaatagaa
    28381 caaatgaact tgccagaaga agcaaaaaat ctaatcacga atgcacggct gaacatttat
    28441 gatacattag ttatgtttgc agatacgcca gaaggaattg gtcctgaatt agttgtggga
    28501 aataatgttt ctttagtcat ccagacaaga aatgaagaag aaacgaagcg cctatttgaa
    28561 agcttagctg agggtggtaa gattgagatg ccactggatg ctgttccgtg ggctcagttg
    28621 tatgggatgg taactgatcg atttggcatc aactggcaga ttaaccaaga gtgacagcta
    28681 aagatgaata ttttctggaa acatcaagca gcaaacggat catcgtttgt ttgcttgatg
    28741 tttttgtgtc tatttcgact atacggtatc ttttttttat tgccagatgc gaaaatatgt
    28801 tccataatag agaagtccgc aaaaaattaa gtaacggagg tcagaagatg aagacggtga
    28861 tactagctga aaaaccttca caagcaaaag cctatgccga aaccttccaa cagcaaaagc
    28921 ggcatgaagg gtattatgaa atcagcgatc cattgtttgc tggtgaagta accatcactt
    28981 atggttttgg acatttggta gatatggttg ctccaggagc ttatgaagag cgttgggcaa
    29041 aatggtcttt agaaaatttg cctattttcc cagaaacctt ccgttacgaa gttccaatgg
    29101 acaaacgtgc acaattttcg atcgtaaaac gagaattgca gtcagccgat accatcatta
    29161 ttgcaacaga tggtgatcga gaaggtgaag ccatcgcgtg gtcgattatt cgacaagcaa
    29221 acgcattttc taaggaaaag aactatttac gtttatggat caattctctt gaaaaagaag
    29281 caatctatga aggttttaag aatctccaac ctggaaagaa ctattttcca aaatataaag
    29341 aagcgcaagc aaggcagaac gctgattggc tgattggaat gaatggaagt ccattatata
    29401 gcttgctact tcaacgaaaa gggattgatg gcagtttttc tcttggtcgt gtacaaacac
    29461 cgacactcta tatgatttat cagctgcaag aagaaatcaa acacttcaaa aaagaaacgt
    29521 actttgaagg agaaggttta attacgacaa acaaagggca gttttctgga aaaatcgtac
    29581 caaaaaaagc atttaaaacc caagaagagt taacgaagga aatagaaaaa cttggagcgc
    29641 atttaggtaa gcaaaacgga cggattcttg aagttacgaa aaaggaaaag aggttgcata
    29701 gtccacgttt attttcgcta tctagtttgc agacaaagat gaatcagcta atgaaagcaa
    29761 gtgccaaagc tacacttgat gctgctcaag gattgtacga aagcaagttt ctaagctatc
    29821 caagaacgga ttccttttac attacagaaa atgagcatca gtatctggtt aaacatctgc
    29881 aaaattataa aaaattttta ggaatagaag aagtacagac aactgagatc aaagcaaaga
    29941 aacgatatgt cgatgcgaaa aaagttcaag aacatcatgc aattattccg acaaagacga
    30001 ttccgactcc tgcacgcttt gcgaagcttt ccaaattaca gcaagcaatc tatttgcaag
    30061 ttttgcgcac tactgtggct atgtttgctg cagattatat ctacgaagag acgaccgtaa
    30121 tgacgggcgt acagcaatta aagctaaaaa gcatcggaaa gatcccatta cagcaaggtt
    30181 ggcaggaaat cttacgcatc aagtcaaaaa agaaagaagc tcagactctt cctcctatcg
    30241 tagaaaatga agaagcagaa gtagaactga aaagtatgga gaaagaaaca caacctccaa
    30301 aaccctatac cgaaggaacg ttgatcacag caatgaaaac tgcgggaaaa acgctggata
    30361 atgatgaagc gcaagaaatt ctcaaagaag tagaaggaat agggacagaa gctacacgag
    30421 ccaatatcat tgaaaatctt aagcagaagc attacatcga agtcaataaa aacgaaataa
    30481 ctgttacgcc aaaaggaatc actctttgta aggcagtagc caatgaacca ttactgacga
    30541 gcgcagaaat gactgcgcgc tgggaagggt acttgagaaa aattggtaaa caagagggga
    30601 caccatctgt ttttttagag aatatcaaaa aattcatcct acatttattg gcagaagttc
    30661 ctaatcaaat cgacaaggta gacttttcag atgagattag cgggacacgt cagataaaag
    30721 cgatggagaa gaaaaacgat caattaggaa gatgtccaaa atgtaaaaag ggaatagtca
    30781 tgctttatcc taaaatcgct acatgcttga atccggaatg cgacttcaaa ttgtggccaa
    30841 cagtagctaa aaagaagctg actaaaacga atctcagaga tttgctttca aaaggaaaga
    30901 caagtaaagt tgtcaaaggt tttactggta aaaaagggaa attcgatgca gttttagtat
    30961 taaaagaaga tatgacaatc ggtttttctt ttccatggat cgaaaacagt ggaacaaaag
    31021 cagttgatgg aaacaaagaa acatcaagaa aaaataatga aaacgaagta gagtaaatga
    31081 aaacaatgaa atagcggaat atttattccg aaaagtgaga atataaatac caataaaact
    31141 aaaaaatatc agtgaatcaa gcaaaaacgt tggggtaggc tgattttata tagatagcaa
    31201 ggagtttttt tatttgaaaa aagtgcattc atttttaaat tatcttatgg ga
//
LOCUS       DACENJ010000007        30737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.007, whole genome shotgun sequence.
ACCESSION   DACENJ010000007 DACENJ010000000
VERSION     DACENJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.007"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            216..1547
                     /locus_tag="IW748_000225"
     CDS             216..1547
                     /locus_tag="IW748_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103161.1"
                     /translation="MELLEKAYSSTELIDSYEYEDLKYIKDQRNYSAHPVVTYNGNTW
                     ELRDITKETCQDVIRKSYEIIFLKNPILGKKVTIEIIQYASRIHAMSVTPEEFHSALK
                     NSYLNKLSEKAKENLCKTAFKFVFQLPYGNEEADRDRESTFRLLNALIFDNKEFYLKI
                     LKEEISKYRFTAESREEINKSLGENQQITYTKGVFLLTFIATHPELQHDFSDETIQNL
                     QISINDFKPKQQVLTEIEYYYRLRSLAVLGSNNEELQNHLNSLLDPILQQKMRVSSLP
                     SSTLETIYNQYLYFGEKEMFIDFMIEHLTDASSFRLADKDMEVLPWIYNKLNQEQFNK
                     LLYNLNGNNQFYKNSNFPPFISNLLDFYNQKFQVNLRKHYSGLLYPNAVTMDQGFQLK
                     DKELKNLYELAESKSDMYLDIIDNIINKSEDYLRKEINSEKELLNYVHKIK"
     gene            complement(1804..2139)
                     /locus_tag="IW748_000226"
     CDS             complement(1804..2139)
                     /locus_tag="IW748_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103162.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(2314..2808)
                     /locus_tag="IW748_000227"
     CDS             complement(2314..2808)
                     /locus_tag="IW748_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103163.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFEL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            complement(2805..3032)
                     /locus_tag="IW748_000228"
     CDS             complement(2805..3032)
                     /locus_tag="IW748_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103164.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(3199..5169)
                     /locus_tag="IW748_000229"
     CDS             complement(3199..5169)
                     /locus_tag="IW748_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103165.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRVETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(5184..6050)
                     /locus_tag="IW748_000230"
     CDS             complement(5184..6050)
                     /locus_tag="IW748_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103166.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(6040..6273)
                     /locus_tag="IW748_000231"
     CDS             complement(6040..6273)
                     /locus_tag="IW748_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1103167.1"
                     /translation="MREEKIVYPVIQNNQENITSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(6285..8441)
                     /locus_tag="IW748_000232"
     CDS             complement(6285..8441)
                     /locus_tag="IW748_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103168.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEAYQKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEGDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNMFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAEDVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSASDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNTRISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIIAVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(8441..8683)
                     /locus_tag="IW748_000233"
     CDS             complement(8441..8683)
                     /locus_tag="IW748_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103169.1"
                     /translation="MVFDREKMLTHADEVLMSGLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTIDRKKGVLQ"
     gene            complement(8793..9131)
                     /locus_tag="IW748_000234"
     CDS             complement(8793..9131)
                     /locus_tag="IW748_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103170.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNEQLG"
     gene            complement(9531..13172)
                     /locus_tag="IW748_000235"
     CDS             complement(9531..13172)
                     /locus_tag="IW748_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103171.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFMRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKGSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDEKGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKGRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNENNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKIAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(13175..15124)
                     /locus_tag="IW748_000236"
     CDS             complement(13175..15124)
                     /locus_tag="IW748_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1103172.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(15166..16224)
                     /locus_tag="IW748_000237"
     CDS             complement(15166..16224)
                     /locus_tag="IW748_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ1103173.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFT
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(16343..16798)
                     /locus_tag="IW748_000238"
     CDS             complement(16343..16798)
                     /locus_tag="IW748_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103174.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(17382..18185)
                     /locus_tag="IW748_000239"
     CDS             complement(17382..18185)
                     /locus_tag="IW748_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1103175.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            19165..20388
                     /locus_tag="IW748_000240"
     CDS             19165..20388
                     /locus_tag="IW748_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ1103176.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            20423..21190
                     /locus_tag="IW748_000241"
     CDS             20423..21190
                     /locus_tag="IW748_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1103177.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            21380..21778
                     /locus_tag="IW748_000242"
     CDS             21380..21778
                     /locus_tag="IW748_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ1103178.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(22084..22479)
                     /locus_tag="IW748_000243"
     CDS             complement(22084..22479)
                     /locus_tag="IW748_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103179.1"
                     /translation="MKQEKRKMKQTQTETINDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(22480..22857)
                     /locus_tag="IW748_000244"
     CDS             complement(22480..22857)
                     /locus_tag="IW748_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103180.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVETLSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(22847..23242)
                     /locus_tag="IW748_000245"
     CDS             complement(22847..23242)
                     /locus_tag="IW748_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ1103181.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(23309..23494)
                     /locus_tag="IW748_000246"
     CDS             complement(23309..23494)
                     /locus_tag="IW748_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ1103182.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(23576..23830)
                     /locus_tag="IW748_000247"
     CDS             complement(23576..23830)
                     /locus_tag="IW748_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103183.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKNNKSFY"
     gene            complement(23852..24097)
                     /locus_tag="IW748_000248"
     CDS             complement(23852..24097)
                     /locus_tag="IW748_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103184.1"
                     /translation="MWTLEGKSFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(24104..24388)
                     /locus_tag="IW748_000249"
     CDS             complement(24104..24388)
                     /locus_tag="IW748_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103185.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(24491..24673)
                     /locus_tag="IW748_000250"
     CDS             complement(24491..24673)
                     /locus_tag="IW748_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAQ1103186.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(24879..25418)
                     /locus_tag="IW748_000251"
     CDS             complement(24879..25418)
                     /locus_tag="IW748_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103187.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLPAGDVKEVA"
     gene            complement(25531..25797)
                     /locus_tag="IW748_000252"
     CDS             complement(25531..25797)
                     /locus_tag="IW748_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1103188.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(25799..26020)
                     /locus_tag="IW748_000253"
     CDS             complement(25799..26020)
                     /locus_tag="IW748_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAQ1103189.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            26240..27058
                     /locus_tag="IW748_000254"
     CDS             26240..27058
                     /locus_tag="IW748_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1103190.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDSLAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            27062..27358
                     /locus_tag="IW748_000255"
     CDS             27062..27358
                     /locus_tag="IW748_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103191.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTLKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(27540..27893)
                     /locus_tag="IW748_000256"
     CDS             complement(27540..27893)
                     /locus_tag="IW748_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103192.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDINDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(27913..28968)
                     /locus_tag="IW748_000257"
     CDS             complement(27913..28968)
                     /locus_tag="IW748_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ1103193.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            29184..29582
                     /locus_tag="IW748_000258"
     CDS             29184..29582
                     /locus_tag="IW748_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103194.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
BASE COUNT         9655 a         5502 c         4620 g        10960 t
ORIGIN      
        1 atcctagaat tattccatta ttagaagaag ttcgaagtag tttttatcag ggaaattatc
       61 gttctgcaat agctgtcaat tacagtgcgc ttgttttaga tttaattgat aaattatctg
      121 atttagcaaa tatttacgag gatattactt ctaagaaaat tttagaagat atagctagat
      181 taagaaaaag tgatccaaat tctccaaaat gggaaatgga actactagaa aaagcttata
      241 gttcaactga gctaattgat agctatgaat atgaggattt aaaatatatt aaggatcaaa
      301 gaaattattc agctcatcct gtagtcactt acaacggtaa tacttgggaa ttaagagata
      361 taacaaaaga aacttgtcag gatgttatac ggaagtccta tgaaattata ttcctaaaaa
      421 atccaatatt aggaaaaaaa gtaacgatcg aaattataca atatgcaagt cgcatacatg
      481 ctatgtctgt tacccctgaa gaatttcact ctgcactaaa aaatagctat ctaaataaac
      541 taagcgaaaa agcaaaagaa aacctatgta agacagcctt taagtttgtt tttcaattac
      601 cttatggaaa tgaggaagca gatagagata gggaaagtac tttcagacta cttaatgccc
      661 taatttttga taataaagaa ttctatttaa aaattctaaa agaagaaatt tcaaagtata
      721 ggttcacagc tgaatcaaga gaggaaatta ataaatcact tggtgaaaat caacagatca
      781 catatacaaa aggggtcttt ttacttactt ttatagctac ccatccagaa ttacaacatg
      841 atttttcaga cgagacaatt caaaatttac aaatatcaat aaatgatttt aaaccgaaac
      901 aacaagtact aacagaaata gaatattact accgtttacg ttcacttgca gttctaggaa
      961 gcaacaatga agaattacaa aatcacttaa atagtctact tgatcctata cttcagcaaa
     1021 aaatgagagt ttcttcgcta ccatcttcaa cattagaaac aatatataat caatatttat
     1081 attttggtga aaaagagatg ttcatagatt ttatgatcga acatcttacc gatgctagtt
     1141 cattcaggtt agcagataag gatatggaag tattaccatg gatttacaat aaattaaatc
     1201 aggagcaatt taacaaatta ctatacaacc taaatggaaa taatcaattt tacaaaaaca
     1261 gtaattttcc gccttttatt tctaatctgt tagactttta taatcagaaa ttccaagtga
     1321 atttacgtaa acattatagt gggcttctat atccaaatgc agtgactatg gatcagggtt
     1381 ttcaattaaa ggataaagaa ttaaaaaacc tgtatgaatt agctgaatcg aaaagtgata
     1441 tgtatctaga cataatagat aatattatta ataagtctga agactattta agaaaagaaa
     1501 tcaattccga gaaagaattg ttaaattatg ttcacaaaat aaaataattt cttataacac
     1561 aagatgcact tttatagcgt atatttactt tcaaattaaa ctaattagtt atattctcac
     1621 aatagcattt aataataaat atactgtcgc ttgatttata tatttccctc ttctttcttt
     1681 aaaatctctc tttattttga tataataaaa tagaaatcgc accaaacaat gaaatggcgg
     1741 aaaacatttc attgaaaaaa ggattgaaca taatgttcaa tcctttttta tagttataat
     1801 caattaacat tcggtgtgtg aaattgaatc ttcgatgctt tcacatggaa aattattgat
     1861 tccttcttca ctcatttccc atgtagtata gaaaggagtt aactgtcctc catactcctg
     1921 aataataaaa tattttccat cattgatatg gttacaacag tacactccaa tagttggata
     1981 ctcgactact aaaaattggt ctggatcaaa ctgttctttt ttcaacattt ctcgtatttg
     2041 aaacaaatga tccttctcta agcaatcagg taattccttt attctttgcg ttactaatga
     2101 cccctctatc gctttccatt catttaaata ttgtttcata acaattcatt ccttttatgt
     2161 taactatttt tcctaggaaa ttgttttaaa acgccactct cccagaaaac gtaagatttg
     2221 gttagctaga atataccttc cagctaacca caattttcca ttactatatt gtaagcttgc
     2281 tttcacttca taaaaattgc tgatatactc ttttcaaatc gaactcttcc tatctgagaa
     2341 gggtatttct tgcgctagcc cctcataagt tccatcttca ttttcccaaa tttctacctg
     2401 aaacactgaa ttattggcta atttttgttg caagagataa tagggttctt cttcatactt
     2461 ttgatcgact tctttaagac attcaatagc atcaatatca atattttgaa agcccaattt
     2521 ttctaggtaa caatcgtacc aagtacccca tgtattatcc agaaatgaga caaatgcatg
     2581 atccatatca tttgctataa aagattcatc ttcccacctg aagaggtaat cccactcacc
     2641 gtttgcaatt ttagttctga tttcattttc tttttttaac tcaaagggat tacaccaaca
     2701 atgctcatgt cgcatccaaa caaaaaattg tccattctca tctattttaa attggcttag
     2761 atcacctttc aatctaaata cacggttctt ttccggtaac ttattcatgc ttcaaccacc
     2821 tcatactgtt ccctcatacc tttgacttca ttaaaaaaaa ctctcgcttt ttcttcatca
     2881 gtaaagtttt tatacaactg attacaataa tgaggttttt gaagtttctg aatacctata
     2941 ctaacttcaa atccttcttc atgttgaatt aggattactt ctgtgtctct acctagaaat
     3001 attcgatcta aaatttttat gtttggtttc attatttaac ctccgctata attatttcac
     3061 tcagtaaggt ttcgctttat gaaatttcta tagttctctt ttcacaaaac gtaccatgtt
     3121 ctctaagact gtttttagtc tcaaatatac atgttttttc ttatcctttc cttatacact
     3181 caaaatcaaa gataaaactt aaagaataaa ctctccaata acaaaattat tagtcagatc
     3241 cactggtctc ctgatctgaa atttattact tcttcctgtt gtcaaatact gtctcaaatc
     3301 aacagaaagt ttttgatcat cttctccttt tctagcagga gcaacttttt tttcatactt
     3361 cattaactct ccactggtca tctttccaat gatagtgtaa ttaaaatcac tttcaaaact
     3421 ttggtaaatt tttccatctg tattgaatat atcagctgtt tcaatatctt ctttgtattc
     3481 attgtcttct cctgaaccga ttgtatcaaa tagaaaggca agacgaattc catcttcttc
     3541 aagcattgtt aatctttttt taattagacc aacggtttca atagataact tatcgaagcc
     3601 gtgaatcata acaatatcat ctttttttgc ttgtgatgtg atgaaatcaa aggtatttaa
     3661 aaattgagct tccataacat tggtttctac tcttagatca cctaagttat agtaaaactg
     3721 taatttgtca cttttaccga tttctttcag tgtcgttctt tgattaaaca ttctagagaa
     3781 gctgtctaat gctgttctca atgtgtcttt taatacaata atacgatcga cttctttttc
     3841 agtaccagat tttttggctt ttgtttcagc attagtcaaa tagctaataa aatccccaag
     3901 aactggcatt gtatcactac tgttcaccaa aagtttcgaa cgttcttcat aattaatatc
     3961 ccaaagtttc tgacttatat aaaagttacc taattctttt ttcaataaaa ctctagttgc
     4021 agaatctaat gttctgcctg ctaacaaata aaaaatctga gttagtttat cgagattccg
     4081 tctatataaa tcaccaatgt ccttttgatt accaaaaagc tccaaagagt taagaccttc
     4141 ttcagccaaa ttaacctttt ctattacact gtttaacgaa ggtttggcaa caaatgttct
     4201 accagttgga tcaccaaagt aatcaaattt atttaagaca atatgaaaag ttcgtttatg
     4261 atttgccatt gcattattag caatcaattg cccccaaagt gatgatgctg agattttttc
     4321 atcatagcct ttaattgtag cgtttttgtg tgatgccact aaaactttac ttgtaaaaga
     4381 atcatccagt gccataaaag ctacaccact tgcgtagcta gtagacatag tgccgatagc
     4441 ccatccattc gcatcatcta aacctttacg aattgcatga tcatttcctg caaaatccga
     4501 actcatagac gtatactcat agacattccc gattggttca cttaaaagtc tatcaaacag
     4561 atcacgttga tctcctccat atgacataag ctttaatcca tacatttttt catcatacaa
     4621 ccgttggagt ttaattagct gttcgctgat tttattagga gaatcggata ctaataggat
     4681 ttctagcttc caatcaataa ttttatttgc catgctgtct agtaaagatg ctgtattcaa
     4741 atccataatc cgcataacag ttttgctaat ttcagcatct gaattttttc cttctaaatt
     4801 attttcataa ctattcgttg atcccccaac attctcgtgc attatttctt gttgatcttt
     4861 ttgtgacact ggcttatctg cttcaattag ataggctttt accccttcta ctctcacttc
     4921 aggaatagct ttaaccatcc aaccaaatcc ataatttcta ttcattccat aagtattcac
     4981 taatttcaca attgttcccc accgcttacc tgtcctaatg taagatggtg taaattgata
     5041 accaactaaa gatgttaaat cactaacaaa ggtattattc actacctttg aatttctagt
     5101 tttttgttct tttttagttt ttctcttttt tttgaaattt aatagttgtg atctacccat
     5161 aatttccatt taatctctca cctctatttc agtgcataaa tatcagataa tatttctttt
     5221 gtttgtttag ctgacaactg agtgacacta taataaagtc ctctactcgt atagttttct
     5281 aaacgttcca caccttcatc caataccatc ttagattgag atttcaagat aagatattgg
     5341 attactgttg ttttcacacc atcaatatat tctgatacaa atcgttcttg ctgatctatt
     5401 atgtcaaaga ctgctttgtt actccttgca tttcgttttt gtattttcat atttcgagtt
     5461 tgtctttccg tattctgagg ttgagatgaa gtgattttga tttcagctaa ggaacgatct
     5521 ctggtatttt gatattgtat tgatttcgat tcctgagcca taacttcttc tggaaaagcg
     5581 gttagtgatg tagccccatc tagattgaac attaatgcta cactcccatc tcgatatacg
     5641 attgttccat cttgtttcac ttttaaaatc tctgttcctc gaaatggttt ctttttcccc
     5701 atagatactc ttgctgaatt aactactgat ttgaacagtc taatagaacc agaaatcaat
     5761 gaaccactag atggatcaat aaacatatac tggctaatta gaataagaaa tccaattatc
     5821 attaataacg ttggaacaaa aataaatttc agtttaatcg tatcagaaac taagtaaaca
     5881 attgatccaa gaacaacgac tgcaacaact atagctgata caattaatct ttttgctgta
     5941 acagaatagg tgctattgac atgcttttca agccattcaa ctctgacaat tgtttcagaa
     6001 ggtttttgat gtaagttatg tttaaatcca tagtttgagt tatctgccat cagatatact
     6061 tcctttcacg ttcaacttac ggtaaacacc tacagttatc actattgcaa caataagcaa
     6121 gccaattaaa gatattgact tgctcattgt ctcgttcgtt tgtggtagtt catttacctg
     6181 atacacgagc tgtgcttcct gtaagtgctg agaagaggtt atgttttctt gattgttttg
     6241 aattactgga taaactattt tttcttctcg cataatagcc ctccttagtt attatcagat
     6301 aagtgagctt gaattttgta ctgatttccg tctggagttt tcacaactgc tttttctcca
     6361 tcaatagaaa tcacttcagc cttagttgtt ttcccatcaa tactgatgac aacatctttc
     6421 ccaactttga actctgtagt tggtgttgct tcatagctac tatttgttga agcagagttc
     6481 gtatttgtcc caatcgaatt ttccactctc gaaatagatg tttgagtttg agcctgtgct
     6541 tgttgattat tatgactaat aaagtaggac gatcccaaga tcgttccaca agccattgca
     6601 gcgacagcaa tgatagtagc catcttttta cttgtaaaat cacgttttga acttgattga
     6661 ttttctgtag caactgctaa tgatttcgtt ttttctgcca tttgagctag atcacttcgg
     6721 gaacgagata agtcttctct taaagcttca ctttgttctg ccaacttttc gctatttaat
     6781 ttataaccct ctgctaaatc tttgacctct ttcattcgat ctttatattc ttcgatagtt
     6841 ttttgttgct ctacaagttt tgtttctaat tctttcgtgt tgttagctac ctgcttttca
     6901 acttcttctt tgttcgcaaa atatttgaca accacatctg ttgttttctc ttttgtatca
     6961 tggaactcag cttcattttc tgactttgtt ttttcgacaa tctctaaagt tttttcttgc
     7021 acgtctgtta atgatttttc aatttctggt ttcaatgaat tgataattcg ttccaccaca
     7081 acttcttttg cttcttggct ttgtttcaat gcatcgtttt taatagattc aattgcttct
     7141 tggtactgtt gatcaagact ttcttcagtt gcttttctat attcatcttc ttgttgttta
     7201 acttctggta aatgttcagc atcataccgt tgctctagtt tctttagttc agcatccaaa
     7261 taagcctgtt tcgccatatc ataatcttta cgaatgatac tggatttttc catcactaaa
     7321 tcatcaattt tagatgcttc tgcttcgtag ctctctttag caaccgatag tgtatttgta
     7381 aactgatttt gaccatttaa tgaggttgct tcctctatcc gcaaaagctc atcagctaac
     7441 atttgctttc ctttgaggat caattgctcc ttcgtcttat ttaattcact gttgatttta
     7501 ttattagcaa tagagatgcg agtgttcata ctctctttag ctaaaaacag caatggtttc
     7561 aactcaggta tggatagcat ctcctcgttt aaatcgacag ggtcattaat ctcatctaaa
     7621 actacaggtt gaaatttgtc agcaatgatc tgttcaaaat ttttttctaa aaaatcagca
     7681 tctaagtttt tcggtacatc acttgcagat gtgatttttt cagcttcata aacataattt
     7741 tctttgtcac tttgggattc gggtttttca tcaatttttg gttgttggta ggtttctgtt
     7801 tgtatttgtg tttcttctat gcttacttct tggttttctg cttcggttgg tttgttatca
     7861 tccacaaaaa gagcttcttt ttcatctggt acatcttctg ctacagcttc aaattcaact
     7921 tcttcagaag agttgaaggt ctcagcaatt cgttctaatc cattagtagc tacttctaat
     7981 tgctctactg cgctatagaa gtctgtagct tcttcaactt ctgattcaaa catattagga
     8041 tcatccggaa taactaataa ttgaccacta ctatccataa aacggacacc tgctaatagc
     8101 ttctctttaa atttttctgg tagatcagct acataattca aacttgactt caatagaacg
     8161 acatactttt cagaatcgag aatagcatca ccctcatcta taactccctc actgttgagc
     8221 ttttgtacat cttcaattac catataattt ttctttggta aatctttggt cagcaacgca
     8281 tcgaattctt tccatacctt tgctttagct ttttttgcct tcgcttcttt ttcttctttt
     8341 tggtacgctt cgagtaattg tggcttcaaa gagagtgcaa ctacaggaac tgtttctgcc
     8401 acataattaa tagattcggt aatatctttg aattttgcca ttattgtaaa actccttttt
     8461 ttcggtcaat agttcttttc tgtttgtcta gcatatatac atatgctttt tcgaatttaa
     8521 taagcgtttc aagtctggct ttttcaatct ttttactccc atttcgataa tcatagaact
     8581 ggttaacatt catattcatt atctttgcca gttcggtacc tttcagaccg ctcattaata
     8641 cttcgtcggc gtgagttaac attttttctc tatcaaaaac catcttacat ttactttctt
     8701 ccctcacttc taaactacgc ttggagcgta gttttttggt aaaaagtaaa ttgaacaaat
     8761 cggcatatta tccaaatttg ttcaattcac ttttaaccta actgttcatt ttttggaaat
     8821 tttgaaacta gatagatacc aacaactgga ataagtgctg atatgatatt agctttttgg
     8881 ttcaaatcat tacctgtttt agtacccatt gtgtaaagca taacagttgt aattacaggt
     8941 ataataaaga ttgcgataat cgccccaaca tattttaatc catctcccca ttttgcatca
     9001 ccaaaaaatt tcattgcagt gaatcctggt ttcaacgaaa aaacgaatgc caagaaatac
     9061 aaaacaactt gtactaaagt tttcatatca ggcgtcattc cgctaaccaa atttttaata
     9121 gaatcgaaca tataaaaacc tcctttttaa tatttttaat aagttaatta tatcatcgac
     9181 aaattttatt tgttcacttg tcaatgatct aactaagaaa aagacgtctc aatatttaca
     9241 tctttatgtt atcataataa cataaatttt agatgattaa aagagatttt ccattaaatt
     9301 atttcacttt ttttattggc taaaaatgct ggtttatcaa gtttttgttt agataataat
     9361 tctgttcttt accagcataa gcccttttta attatacaat tttgaacagt aaatatttaa
     9421 ttcatttata gacaatttaa aataattttt ttgcataaat agaaattttc cttgacatct
     9481 tcagttagaa atcaaagtaa aaagaaggag caaatcaatg ctccttcttt ttactcgtct
     9541 tcagacttat tttcagttga tttttgatta ccgtatttta caatatagtc cttgactgac
     9601 atggtagatt tcttcactac tttaccagtt ttggatgctc ctgtagcagt agccttggca
     9661 atcttagccg ttcctttacc tattttaata gcttcgtttt tagcttcata ggttatccct
     9721 gctagactac cttttggagc ttttgctaaa gctttatctg ctgaggtgta aacattttta
     9781 ccagtttttg atagatattc aacggctgat ttagctgttg atggtttctt ggttgattga
     9841 ttagatttcc ctgaattatc tgaagtagat ttagaattat ttgttctatt attttcattt
     9901 gtcagttcag gttctctaat tgattgattg tctttcgaca ttcctgatcc aatatttgtt
     9961 tcatatttat ctgtttgatt aacattagga gtaggaatga actttttctt cacatctgat
    10021 tgttctttat ccgtttcttt tgttagcgat tctctgcctt taagtgctgt gtcattccga
    10081 atagcactat tatcaaagtt gctgttaagc tctcttgact gactttcacc agatgctcca
    10141 tctcctaatt cccgatgaac tctgtctgac acgtcctcac cactcatttc attcctcagt
    10201 tcttgatgag tttgatctaa agtaatctcg tcatcttcat tctcaggaat tgcaacagca
    10261 attggcgtat gccgttgttg ttcgagttct ctatttcgat ctgccacaag cgcatctctc
    10321 gtctcaatga tattcccatc ctcatcgact aaatcgccat tcacattata tccatcgctg
    10381 tgtttaccaa gtttattacc aaactcatca acaagatttc catcttcatc aactaaacgt
    10441 cctttttcgt caactaaacg tccgttacta tcctgaccaa aatcaccatt tagcaacctg
    10501 cgtccttttt cgtcaacaag atttccatct tcatcgacta agcgaccttt ttcatcaatt
    10561 aaacgtcctt cgctatccat accaatatta ccatcttcac tatcttccaa tggtttacca
    10621 ttagtatcta taaggtttcc atcttcatcg accaaacgcc ctttttcgtc aataaagttg
    10681 cctttagcat ctttaccgta tttactacct tttaatattt caccattttc atcgacaaag
    10741 tttccgtttt catcaaccat acgtcctaaa tgatcaatat attgttggga attattacgt
    10801 agttgatctt gacgaactaa atctttgaaa gaggtattat tgattgttga accaactccg
    10861 ccaccgttga atgacattcc ttctggtgtt tcaggaatta ctttatcagc aatggactta
    10921 gcatcagcat tgctatctcc catattgttc aaacgttcag ccattttttg cgctccaact
    10981 gctccaagac ctcctgcaac catatctttt actgagctgt taccaccatc ataataggga
    11041 tttttagctc cttggctttt cttgattgct ttagaaacgc cgtccacaac tcgcacagca
    11101 tcatttctat taccaaataa taacttagca cgttcatcaa acccaaacgc tacttcaagt
    11161 ggtaagccaa taagcatatt aatcaaatta tttacttttt cgcctttttt aaagactaaa
    11221 agatatccta cacctaaaat catagcagat cgaacaatat ttgctacgcc atcgccaact
    11281 aatgtattag gaatgaatcc cacagcagta tcaccaaatg cacgaataaa agtaatcaga
    11341 aagataggta attgtagtcc aatcagagat gttgtaacaa cacctaatga aattgccatc
    11401 acacctgctc ctgatgcaag tgatcctgtt agaactactt cccaaatccg cataaattca
    11461 cgtttaaatg ctttcacgat atttacattg aacatcgccc aagcacatgc taagtaaaga
    11521 actgctaaaa cgattgattc agcgttcatc gctagccacg agccaaactt acccatcgct
    11581 ccatgaccaa ctaaggatgc tgtacgccat tctgttttta ctgttgacat attcttagca
    11641 ctgccttgga ttcctgaagg agcgatatta tacgcataaa acttagctcc tgatgtatca
    11701 agtgccgatt ggagcaataa agctaccgac tgtgtgctaa aagttgcatt gttagcaacg
    11761 ccccaaggtt gagtagcatc aaagttatgc gctgttcctt ttccatcagg cttgcgattg
    11821 tcgtctgtca cattttgatt tgctgaccaa atatatccat ttaaattggt tggtttctta
    11881 attgcacttc cttcttttcc atcccctaaa ttatagcctg tccaaacagc aggcgctctt
    11941 cccatataag catcattttc ggcgacatta acatcaccgg cataagtatt aatattaaaa
    12001 gtatcattcg atgaccattc ttttaataga gccataccga ctgaattaga atctagatca
    12061 ttattttgtc tggcataact gctagtattg atcgctctta tcattgcaga ttttttagat
    12121 ggttcataat ctgtatcaac gaacttgatt tcggatgtag gctttccgtc aggtacatca
    12181 ccaccagtat cagatgatgg agcaagattt tgagtagaag cccaaagtct tgtgttaatg
    12241 atgtagtcag aaacaacatt tgttggtata tctccatctt tttttgctga ctggatgccg
    12301 ttgatataaa ctcccaatag cgatcctgcc actggtaaga atataaacaa tacaaaaata
    12361 cgaacaatcc atttttcaag tactggtgat aatcccttcg agttaacacc atctttcgac
    12421 aataatttca ttccttggta acctagtatt gctgtgaaaa agagcataga taagcttgct
    12481 atcgctttaa ataattcatt atttaatccc aaattgttaa gcaagctagt taatgcttta
    12541 gataccggat tagatacatc cgatccacct tctccccacc ccataagaga aaatatattc
    12601 atactcatga aaaattttcc aaggagttca tttatggatt cagcaatatt agaaaggatt
    12661 aaactaattg agccgataaa actaccaatc gtcctaccaa tacttcctag aatggatgat
    12721 gcttgactgg caatagcatt tcctgagact gtttcaatat atccgtagta actataagtt
    12781 gctaaagtag atgctagttg agtacctgca ttaccccctg caatcgaatt accttgttta
    12841 gaaatatcag aataaggaaa atcggcactg ataccacctt gtcctaacaa attcatttgt
    12901 ccagttttag cggcactatc attaacttct ttatctccca ttaagatacg agcatatagt
    12961 gtccacattc cttgttctgc cggtttactt tccgcataag caacgttctt atttataact
    13021 gtttcttgtt tgctgtcttg attgtcagga gttggatcat tcattccaaa aggagcgcct
    13081 aaagtaactg ctaatactcc tccaataact agacctctta cgacccatcg ttttattcta
    13141 agattttgta gtaaatattt cttgaatttc atagctacgc ccctccaatc gaaaatacaa
    13201 tcttgttttc tttactttca gcaacggaag gctttgcaat aatttcagca ttagaatcac
    13261 cttttccttc aaacgctccg gcttcactca acttaacatc gaatattttt gcgtttggat
    13321 aagtaattat tgcttcagta atcgcttctt caaactcaga ttcaactaaa tgcaagatat
    13381 tccttgcacc ccctgattta gaatcctctt gtgaagccat gtcaaaaact aagtgcatgg
    13441 agagatcgtc tgcataaaaa ttttgaccat atactctttc tttcctaggt ggaagacgta
    13501 agtctatatt taaatgtttt cgtactaatt cagcactctt caacatttca ttataagcaa
    13561 tttccagaag agtttgttcg gataaagccc aaaatggaac tattcgtcca aatcggttaa
    13621 taacttcagg ctttagacct tcaccaatca aggcttgttt gatccctgat gcgacttttc
    13681 ggtattctct ttttagttca gtgctatatt cattagaatt ttccttgttt gtaacacgag
    13741 atagtgagct atatctgttt aacgaatcga acatctccgc acctgcattt gttgtcgcaa
    13801 ataaaattgt atttgacaat gaaacggtgt cgtgaacttc tcgatcatcc catcctcttt
    13861 cagtaaaact tatcctacct tcatctaaaa ttgataaaaa ggcatcgagt gtttctacac
    13921 cagctttctc atattcatca cacacaataa tcgcagaagg atcatgcctt acatgagtac
    13981 ctatgagtcg tctaaaacgc attgcatcat ctttggtttt gtagtccggc atattaacta
    14041 agagaacttt attttcatcg ccataaagtc ctcttgccac ggctttagcc attgcagttt
    14101 taccgacccc tgttgatcct gtaaacagag cagttgcttt tggttgcttt cgacctttaa
    14161 aacgatcaaa tccaagcttc atacgtttaa acaacattgt tatagtataa actgctaacg
    14221 gttgcccttt tatggacttt aattcctctt gaagtttttc catatcaaaa tcagaatcta
    14281 gctgaatatt atacttacca aatatttgca atagactttc tcgattataa gcatttccgt
    14341 ctactcgatc ttttgcaatc aaatcctcca ttacggcaat tgatttagcc ggttcggaca
    14401 aatcctcacg ataaagcttg tttgcttgta cgatttcttg aagtaattca tcaggaatcc
    14461 tttgcgataa tttaaaagaa gcctgaggat catctttaac agaggatgta tttagccatt
    14521 gtctcaaaat atcgacgaca acatcgcttt ttagttcatt caattctaac ggttgtaacc
    14581 gtctccctaa ggcatcatct cttttgatat agttttcata ttcttttctt gtagtagcgg
    14641 taatcaattg aagtgattca tttgctaacg gttcttttag cgaatcaccc ccaattttat
    14701 tgccactccc aaaagagcta acaactttat gaaactcgtc aatgaagagg acaaattcat
    14761 tttctttgtc ctcagatttt aaaattcgct catattcttt caatcttgga attaatgttt
    14821 ctaaacgtgt ttggaggaca tcatctcccc caacacgcaa ttgtccgatt gataatttgt
    14881 atacttgtac tttctttcca tcttgtcgct gtctttcagc ccattcactt gcaagtgctg
    14941 ttttacctgt tccggcttca cctaacaaga tagcttgcgg tgtttccggt ctattcaaga
    15001 tcagtgacaa ctcatggagt tcgtcatcac gtcccactaa cttatgtttc ggtggtttca
    15061 ttggtgtgag agcatgatta aattcagttt gaatattctt aaaaaactcg gtactatttt
    15121 ccatctgttt gtgttcactt cctctaaaat aagaataatt tacttctagt cagtcagagt
    15181 taggtactta aatgtcgtct tcatgtctcc aaaagaatct ttggtaaagt cagcattttt
    15241 gtattgatca atcaattttt gtgtatcatt gattgcttta atttcggtct ccattcgact
    15301 gtctagttga cgaatacttt cttgcgaatc tttatcgtca ggattttgtt gtaaacgttc
    15361 cagatactca gcttttgtgg cttcaatttt cttgatgtta gtatttgaag catttaactg
    15421 atcttctagc ttatttacaa cattattctt acttgtaact gctacaactt ttgaataatc
    15481 aatatcgcca tctttggtaa gaaagcttcc ttgatagaca ttagttgaat ctgaatatgg
    15541 atttattctg aaattgatat tatcaggagt attaacaggt tttgcttttt tcccaaaaga
    15601 aattactcct tcattattaa catcattcat agaatattga gaaagtgctt ttgtaactga
    15661 atctgcttca ttcacattga ctttggtatc atcgtcagct gtatcatcac cagaaattag
    15721 tgatacttgg tttgcaataa aaacttgata agctctttca tctaatttac cttttaggaa
    15781 taagaaagca tccccatcag tacttaacat cccatagctc atttcaagct tcttaggacg
    15841 atctttttct tttgttgcca tatataagtt gtagtttttt ccttgcgtag ataacaactt
    15901 tctagcgttt tgcgagtaac cgagttttac tactgttaca tctctgtttt gatctgtcca
    15961 cacatctgaa atagtgatct ctgctcctga tcgagcaaac tccaatgaag agcctatagg
    16021 tgttgtattg ttcacgagat aataattata gtttcctaat tgataaatgt agctacccaa
    16081 gcctgttaat aaaaaaagca ctcctagaaa agttgcaagc ataatgcgta atccgtgacg
    16141 tgttctctta tccttatcac caagcaaccc tccaataata ctgtattgat ctttggtaga
    16201 tgttgaattg acgttttttt ccattagttt gtctgccccc tttcataaaa gagcaaacaa
    16261 tcagacgttt tttcggtaat tctaggttcg ggattttgaa tcatttcggt atattcttcc
    16321 ccattaatat ttaacacaaa aattatcttc ctttcttcgt tcctttcttt tgctctttat
    16381 tttccaaata atcaatgatt tttgctattg taccaagata gtaattcccc tcggctttgt
    16441 tttttcggat tttgctaacc attggagtag gtacaatctc ttcatctctc aattcactga
    16501 aagatttttt acctagatat tttccaataa cagattttaa aaaacgttca aaatctttct
    16561 cgtaagttgt tttcttgcct tttagttcat ggttcatatt gtcaacgact tttgcggttt
    16621 caataattgt tttagttctc agcttaataa tttcatctgg aattttcaat cctcgtaatt
    16681 tcatcgttgt actaactggt aacccatgct ttactgcatc agcaatacta aaacttttta
    16741 gatcatctag taagctctcc atatagttaa caaattcatg ccaattaata gcattcattg
    16801 tctcacccct tcattttttt aaaatgaggt tcgggcgtaa cacatttatg ttattataat
    16861 atcataataa cataaataaa tcaatcagca aatttagata tcccttaaag tatcagcaat
    16921 tattcaacaa gatatttatt ttagctattt cagtcatcat gaaagactaa ttagcaataa
    16981 aataactaat attatccatt tatcagacaa aaagaaaaaa cttatctaat tactctatat
    17041 taacttttaa caaatagttg acttgtccaa aaatgttacc ttcaatctca caaagatatt
    17101 aagatgtttc ctaccaaaac tgatatcacc ggacttttta ttctgtgttg tccaaaagtg
    17161 ttacctttag tcgatcaaca aattaaaaaa aattgttttt ttcttgactt gtccaaaagt
    17221 gttacccata gttgttaaaa tttgtataga ttagccattc tttcaaatat tatttagact
    17281 gaaacttgct tacaaaaaaa cttcttgcat ttattgtcca aaagtgttac ccatactttt
    17341 ttaaagaagc aggttgtatt aaaattacaa cctgcttctt ctcaatcttc ttctaaccaa
    17401 ttatgcattg aaatggttgg caagtttgat tctggctcac taaaagtgaa tctgagccga
    17461 tcaactcttc ttcctttctt tgcatagatt ttttcataat caaaagtttc aaatacctgt
    17521 attccgtttt catcaggagc taaaaattct tctttcgcct gctctagtat tcttttgtca
    17581 atatttgtca ttctataact tgaaggtata cttaatgctc ttctaaaatc ttcaactttg
    17641 actacccaat aaccatttcg ccactcttta tttctgtatt gcatgagatg acgatacaat
    17701 tcttttgtgt agctacttct tagttttgta aaattatcaa gttcaaaacg agtaaagttt
    17761 ttgccaattg agtttaagat aaattcaaat tcctcatttg ccttaattgt aactgtttcc
    17821 ttctgtttat ctatccggaa tttttgaaaa agccacagct gttcatagac gtctgaatct
    17881 tcaaaacggt aggttaaagt acctagtttg tcactcaaag cttttaacac agaataaaaa
    17941 ctgtcctttt ctcttctgtc gtatttgacc aaataacgaa agaggttgaa atcaaaagtt
    18001 acttctgctg ttccttgacg tttcattttt gataaaatag cccaaaacac atccatttca
    18061 acaggcttaa atcctcgcaa agctgttgta ttcagaccat tgttataacg cacaattgca
    18121 tttgtttgtt ctgatactaa atctacatca ctatcaataa tcttttcggt aacttcttga
    18181 gacattgatt actctccaat cttctttttg cgctccttta atcatgttaa tctggtacta
    18241 attaaaggaa acaatttttt ctgcttgtta cctaaattaa atcacaacta aaggtaacag
    18301 tcaacaaaga tttgttacta atctaatttt ctattttttc gccttccaaa tcaagaaaac
    18361 cgaaggtaac gtcttatcct agtgtgatac ctcaaatgtc cacttttgtt acctttactg
    18421 tccaaaactg ttacccttac tgtccacttt tgttaccttt actgtccaaa agtgttacct
    18481 ttatacccct ttaatactta ctctcccaag agattaagac tcccctaaca gatatattaa
    18541 cagatattta acagatatat taacagatat ttaataaagg aggattactc ctctcttatt
    18601 tcaaaatctt aaaaataaaa aatcttaaaa aaactaacac tatctaagag tttattttgc
    18661 ttgtgtaact ttgaataatt gaaaaaaagg aaaaggagga tgcttcagct tcccctttta
    18721 tttatattta taatatatat tttaatcttt aaaactttag caacctccaa aaaggcttta
    18781 tatcaatctt aaatagattt tagggtggac ataaagcgat gcgaacatgg tcaaaaagcg
    18841 atgtgaatgt ggacaaaaag cgatgcaaac gtggacaaaa agcgatgtgg tattccgaat
    18901 atatccaaaa aaagaaaaag tatgtgaatt tatttctaaa aactagaaaa atatttgttt
    18961 tttaattttg gtaattttta taatttcgta ttttattgaa aaggattttt attttgaaaa
    19021 tgttctaaaa tagcgatttc tgctgtattt tcaaagaaat ttgattggaa aaaataaaat
    19081 gtgttttact gacattgagt tatgttataa atttacgata catttcttgt tgtaacgtat
    19141 cgaaaacagg tctatactta ttcaatggaa tcgtaccagt tccaagtttt taagctcgta
    19201 ccaagcttag aaagaagagg caatcgtatg aaaataacag aaaagaatgt aaacccgaaa
    19261 tttgaaacta ccgaaagcaa tctaaaaaag tttgatttaa ctgttacttt atcgaatcaa
    19321 cttgtagata gcattgttga aatggatgct atgccacaaa aaatttatct ttacgctata
    19381 ggacatcttg accctaaaaa tccgcctttt aataataccg tcaaagtatc taaaaaggct
    19441 ttatttgcgt tgatgtcttc tagttcatct tcttcaaaac attcaagatt aaaacaagtt
    19501 attgaaaaat tgcatcagca atctgtcatt aaaattcaaa gaactaaacc taacggtaag
    19561 actgaataca aagtcatctc accgctagaa attactgaat tcaatgattt ttctgatgca
    19621 gtatctatta aatttagtag ttcaattatg cctttattaa cgaacttaga agatcatttt
    19681 acgcaatata catttgatca agtagttaac ttgacaagca agtatgcgat cagttttttt
    19741 caattattca ctaaagattt taatctgtat gaagcaaata gagataactt aacatggagt
    19801 acaaaatcac ttgatagatt aaaaaatcct gttttctcca ttgcagattt acgaaagaaa
    19861 tttagtattg agaaaaaata taaagctttt cgagactttg aaagacgtgt aattaaagaa
    19921 ccgattgccc aactgaataa atacactgat tttaaaattg actatgagct tatcaagaag
    19981 ggtcatactt atacaaatat aaagttcttg cttacacgta ccatgtcagt tccggtacct
    20041 tataagcaag aacaaagaga ccctgcttat ttaaaaggcg cagaagataa aacagagaaa
    20101 atgaatcaat cgtacttgaa agctatgaat agcaaataca ctaaattact aatcaaaaac
    20161 tttttgatca gttctgccga cttatgcgat cttaaactta tgagccgact tcagaataaa
    20221 gtttatccgc tttatgatcg tttatccaaa gatagaggat ttgaatcagt tgtcaatcac
    20281 ctatcttacg ttgctactca taaggaggat tacacaataa aaaatactgt taaatatctc
    20341 gaaaaatcaa ttacgagtta tttgccttat gtgaaccttt atgattaaca actgaaaaca
    20401 tgcgtgaagg aaggaaaaac ttttgaaaaa agatgaactg acaaagtaca atgaggagtt
    20461 cttagcaaca attgacatga aaatggattt aaagtcgtat gaactttatc aattgctgtt
    20521 cagttatatc tcacccacaa agctaaagaa aacaggcgta gtagatttac ctgttaaagc
    20581 gattattaat tacatggatg ttgatcaaaa tcttgaaacg gaactaaaaa ccatagttaa
    20641 cactttacaa agacaagcag tttatgaaat atcgaatcta gacaagaaat ataagacgta
    20701 tactactgta tctgctattg atcggattca ttgggattta gaagagcata gtgtatcttt
    20761 cagtttttca gaagatgtaa ttcccttaat tgtctcaatc aaaaagaagt atccgttctt
    20821 tgtttttaaa gacttagcat cttttcaaaa aaaatatagc atcgtgttat acaaatattt
    20881 ggcgatgtat tataaaaaat atcttatcgc caaaaataaa ggagattttg agaaagctga
    20941 aatatataag aatcccctga ttgaaatagt taaaattcgt gagattacga aaacagaaaa
    21001 tgagtataaa acaatatcga attttgatag acgtgtttta agggaatcag ttgaggaaat
    21061 taatgaaaaa tcaatgctga agattgattt tgctaaagtt agaaaaggca gaaattacaa
    21121 ttttgctgtc cagtttaata tttctagtga gtttgatgaa aaaccttata gagaaaaaaa
    21181 attaggttaa tgcaaaatat atcttactcg tttgaagatg cttcagacgt atgctaagat
    21241 atgtagtgcc ttgggagcaa ggtattaatc tcctttagag gttaattagc ttgtgatcgc
    21301 ctatcaattt tttggtaggc gattcttttt ttgaaactaa aaagcaaaaa ttatttttta
    21361 attagagagg tgcaacgcga tgaaatactt tttttgtgca gtattcgaat ggaaaggaaa
    21421 tgaatatgca gttaaattcc cagattttcc agacttatat acgcatggtg aaactttaga
    21481 agatgctttt gagatggcgc aagaagtatt ggaaggatac ttggtgcttt tagagttgca
    21541 agaaaaagaa attccatcgc ctacgcctgt tcaagagtta aatgcaagaa tcaagcgatc
    21601 tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa gttgctaaag ctcgaaacaa
    21661 gcaaaattat gatcgaaaat cagtgactgt ggaacactat ttaaatgttt tagcggctga
    21721 agagaatatt aatctgtcga aagctttgaa tgatttgttg agagaaaagt tgatttgatg
    21781 cctctcagat gatcatatta agcaaaaaca gcttgtttta ggacgtatgg atttaagaaa
    21841 gacaaattat caaagaatat attaaaagct ctcagaagcc ttctgagagc ttttaaattg
    21901 ctatgtattg tactgcatct gctcaaattg attttctttt catgcttatc atgtatctag
    21961 ttttccatca agtaattttt agatatgtat tgagtaataa atagcaaatt tgcttgatat
    22021 aagctcgaat taggtgcttt gtaatgtgtt aggataaatt atacaagaaa atgtcaaaag
    22081 ctctcagaac gcttctgaga gctttttatc ggtaggcttg tttaacgtga tcttgaaaga
    22141 tacccttttg atgtttctct cgatccctgc atcgctaatt actaagtgtt cttcaagtgt
    22201 gatgtaggta tataaattaa gcatcaaaat ggtgtagata agcaaaaaac tccctaaaac
    22261 taagtgttga ttataaaaat ctgacttata aaaaagaact gagaatgtta gtgttgcttc
    22321 ccaaaaaaga aacaaaattt tgtatttcca aagatagtgg atcaatcgct gtgctttact
    22381 catctcaaat aatttataat aacctgctaa ttgatcagca agcgtatcgt taatggtttc
    22441 tgtctgtgtt tgtttcatct ttctcttctc ctgtttcaat taatctaaaa tttcagattg
    22501 agcaagcaac tcattgtggt agtcggtttg aaattcaaat aacaattttg ccttcttaac
    22561 gaggtttaaa gcgttcgtta gtgattcact taatgtttca acatctttaa ctgattttgc
    22621 atgtttcatt ttttcagtgt gcgtatagca gaattcaggt attgagttag gggttgcctg
    22681 ttcaataaac atctcaattc gcaagttatt atcctcaaaa ataattagat cattggcaaa
    22741 gtgtttggta ctaacgctaa tgccagttgt atcttttaat agattattga ttaacgttcg
    22801 agagaaatag tttaagtgtg tagctttcat aggttcggtc tcctttttac ttttcaagca
    22861 actcttcttc tagtgctttt tgaagtagtt gagagaagtt tatgtttttc tccttcccta
    22921 aaagaactaa ccattccgga acactaacat tttttctaat tgtttttgta tttaagcttc
    22981 ttcggaaaag tttaatgttt agttggatga ttactaccac ttgattggct ttcaaagtta
    23041 attcttccac tgctgatggt ttaggaaatt ctgcatctgg tacactatat agcgctaatc
    23101 ctaaagcatc cttagcgttt aaatatgctt cttcaagact atttccaaag gtagatattt
    23161 ccggtaagtc agggaaaacg atgttataag cttctccttc tttctcgaag attgccggat
    23221 aaaaaatatt ttttgtcttc atttcctcgc tccttttcta gtgtaagaga ggatagtatg
    23281 cctaatacca aataggaagg caaggctgtt aaagccctgc ttgcttccag atattagctt
    23341 ctgttcctag tggtacatca ccattgtgga tcggaacaac gaccgttctt ttagtcttgg
    23401 aattataaaa ttttgcatga ctgccagttt gtccagtctt tttgaagccg ttagatttga
    23461 gaatttttac aatttctctt gctgtcttag gcatagctac cctcccttta caaaacctat
    23521 tatacacatt gttgtgtatg gaatcaatag aaaaagagga tgttttattg caaaactaat
    23581 aaaatgattt attgtttttt tcagcaaatt gaatgttatt agcgttacaa atattcatga
    23641 tttcccaagt atcatcagaa gtataggtat ctaagaattc attgacagtt acggttgatt
    23701 cgtatggttc ataagtcaaa gcgttctcca agtcttttga tgaaataatc aagacttttt
    23761 caatttcctc cccttcttca tctatgaaaa catattctaa agtgatttgt tcttcatcgg
    23821 tataattcat attgtgttct cctttcttgt actaagaaaa atggatattt ccaattgcat
    23881 cccactcggc accattgtgg tataggacgt gaccgagcgt tacgcctttg aaatgttctt
    23941 ctaaatacat ccaaacgcat tcaaggctag tacctgcttt gagattgtag aagtctgcat
    24001 ccaatacctt ttcagaagtt atcggatcaa tgcgagttgg tacttcaata aattcagtcc
    24061 aaatttctgc aaaagactta ccttccaaag tccacactcc ctatcaatta attccaaaag
    24121 caaatagaga aatcaagcga ccgtcaatag tcacttcaac aactgaaaat gccgttgaaa
    24181 cagttttaac atctacactt tccaagttcc cgatgaagga atctttgtgc tttagaatag
    24241 ctttctttag ctgtctgtta tacgcttgcc gactgttaaa ctttttgaat tctcgatcct
    24301 tgatcagctg tttaacgatc cctgcattaa ttgtataact ttctgcaata gcaacgtgag
    24361 taggttgctg taaagtagct gttagcatct tgtcactcct tcctaaaagt taattagctt
    24421 gtttacaaat acattataca catcagtgtg tataatgtaa agagaaaaag gtgctaatgg
    24481 atcagctgtt ctaaacaaaa gaaaacgata aagtaacaaa taaagtcagc aaagttaaaa
    24541 aggatgaaac ggctagtccg gttgctttgc ttgtgtgatt ttcggctttc ttatagatca
    24601 aataggaaat tatcagtgat attccagtaa agaataatgt actagctgtc actaaaagaa
    24661 ataggttaat catacgatac actccttctg taatattggt atcagatacg tttaaatgcg
    24721 tttctgagct gttttggatc agatggtgat gtttttagcc ctaccctaaa aacaaagccc
    24781 agaaaagaat ttaaaagtgt tcgtacctaa tacgtttata ttcatattca tatacataaa
    24841 cataaacgta tacacgttta tgtttatgta tttaagtttt aggctacttc cttaacatct
    24901 cctgctggta gtcgagtgaa ccgagtgagt gatccatctt gtagtcgaaa aataaagcta
    24961 cgtttgagtt tatactgata gacagcgaca tacgatccat cccagattga gtaagtttta
    25021 cctgcaacta ataagtcatc aagttttggt ttattgcttt cttctttaat tctatctagt
    25081 tgtcgcttaa ccattttttc ttgatcaccg acaaagaatt tcaaccattt tttcggaatt
    25141 ttcttaaagt aagcaggact gtcatttaag aagtagattg gtttgtacat ttcatctcga
    25201 aagatgaagc tgtcaatatc gtaggttcct tctgcatctt tgaaaaggat aattccatag
    25261 gtcttcgtta ctttggtatc tacgatttcc aaaccatttt tggcgtaccc tttcttttct
    25321 tcgatcactt catttgctga tgaataatca ccataccatc ccataaaaaa tcgctccttt
    25381 tgaaagttaa ttagcttgtt tacacttata ttatacacac tggtgtgtat aatatcaaga
    25441 ggatattgtt cttttttagg taaagtggga tcgtaaacag tgattttgtt gggttaacaa
    25501 aaaaacagac aagccttatc agcttgtctg ttacttataa acttcacttc tgtgaccaat
    25561 agacaatata agaattgtca cttcatgctc gctaatatca gctattatac ggtatgcacc
    25621 taccctatat cgccagtagc ctgatttatt agctgttaga cctttaccat gctttcttgg
    25681 atcatcagtg cctacaagat ttttctcgat ccagtttagg ataacctttg cctgaaactt
    25741 atccattttt tttagcgctt tttgagcttc acgttcatat cttacttggt aagtcatctt
    25801 ataagcctaa ctcttttagc atatcttcgt gggaaatact ttgatcgttt tcctcatgct
    25861 ctttcattag ttgcttgtag gtggcaaagt ctactaacgt ttctgctgtt tgcagggaat
    25921 tactacgcat aaattcagaa atgttatctc cgttgaattc agcaactttt tcaagaaaat
    25981 ctttttcttc tttagataca cgaatactta ttactggcat agtttatcaa ctccttttca
    26041 attacattgt actacgattg tatgacaaat gcaatagtta tatatttgaa tatatacata
    26101 aacatataca tatatatgtt tatgttgtag aatataaaag caaatataaa tataaacacg
    26161 tttatgtata taaatatata cataaacgtg tttatattgt ataatggagt tacaaaaggt
    26221 taataaggga ggtttaatca tggaaaagaa gttgtcaatc attactggaa acattgaaaa
    26281 aggtggaaca ggtaaaacaa caacaatcta caatctaggt ggatatgctg tcgaaaattt
    26341 aggtatgaag gtgctgtata ttgatgaaga taagtcgcaa aacctatcaa aacgatttcc
    26401 tgatgctttc agtaaagcga aaagagaaaa tacaatcaat catttatatc ttcatggatc
    26461 agctgaacct ttagctataa atgagaacat ggatatttta gtagcaggtg aagatttaaa
    26521 acaagttgag atgcaaatgc gtgatatgcc taacaacaga accctactgt ttagatggat
    26581 cgttgttaac tatgaacagc ttaaagagaa gtatgatcta attctgattg atactcataa
    26641 tgatgcaggt ctactagctc aaaatgcttg ggctgtatct gatcttgtcg taggcatttc
    26701 tgatccatct aaagatggtt ttgaagcttt gctgaagctt ggtaaagata ttgattcact
    26761 agcaaaacag ttgattgata ttaagactgg cgaaacactg atggaaacta aatacgctac
    26821 gatcggtagc aggatcaaac ataatactaa ttcaagtcgt gagttcctag aattggtacc
    26881 aacgttatct aattatcttg gatatgtaca agagaaggaa ttgcttaatg caactagctt
    26941 agaaggtaag aatattgctg agttcgctaa ggataagaat atctatcgta agaataagga
    27001 tttctttgaa agcacgttta cgctatacaa caaaataatt gatacggcac aagaataagg
    27061 agtgattgaa gtgaaagaaa atagatttaa aaaattcgct gaagctgaag tagaagaagt
    27121 tcctgaagtg gttgaagcag ttgaagaacc taaacaaaca cttaaagaaa caacaagttc
    27181 tttggttgct aaactaaaaa gtgatagttc tgaaaagaag aaaaattatc aattcacgct
    27241 tcaaccatca caaagaaaga aactatctaa aatcagcgaa aagttaggaa ttcgatcaga
    27301 ttcagcttta cttggtgaaa tgatagataa cttgtatgat gaagtttttg gtgagtgatc
    27361 agtttaaagc tgatcacttt ttttattgga acaagcggtt aggtatagct taactaaatt
    27421 tcgtatacgt gtttatggat atatacgtat acatgtttat atacatgtat acgtatatgt
    27481 ttatgtttgt atttgtacat aataaaaagg ctaggtattg taacctcgcc tttttatttt
    27541 taaagtaagt ctttgttact tggtgcattg aaccctttta gcattggctt ttctttcttt
    27601 gctgaatgat cggatgtaac cgttgaagga tgctcttgtt gaatagggtt atgatcagtt
    27661 ggtttattct ttacttctgc tactttgcta tcgtttatat cgttgcctgc ttcaataatt
    27721 gtaacttcat tgttctttac acgccgatat agattcaaag cttttttgat cgtctgagag
    27781 tatttaacgc ctagtgtttc atattcagat tcaagaaact ctgctaaagg taactcgcta
    27841 accttttctc tattgattat aactacataa cgctctgatt ttcccttatt catgttgatc
    27901 tccttcgctg ttttattgag ttaaaccaaa cttgtaaaag ccttttacat tagaaaattc
    27961 actgtcttca acaaacaagg tatgcggaat tttatctttt acaagttgtt tgtcgataat
    28021 agaagcccca ccaccagtaa agacaacttt atctaaggtt gtgaagtttt tgaatgtagc
    28081 taaaacttct gcaacaacca tattggtgta tgtttttatt tctctttcaa ctacttctga
    28141 aatatcaaaa ctttcatttc cgcttggctt aaatagatat acgccttttt ctttgccttc
    28201 tcggataatc ttttcaagtt ggaaagcatt aggactgatc ggaaggtcaa gcatatttac
    28261 gatacgatta tataaagtaa aaataccggt atccttttgt tcacggttgt tttcgtcaat
    28321 ttcaaaattt tggacaacat caaaaagttt cgtgccacca ccaatatcaa caaaaccgat
    28381 cttctgactg atcaatgatt catcttgtac actaccatca tcagcaagga ggaggttata
    28441 tagtgttcca atcggctgtg gcagtaccca aacggtatct acgtttatat tataagcttt
    28501 accgtcaact tcaactgaat gttgtcgtgt ggcttgttgc atcacgtatt tgacgattgt
    28561 ttcggtatag tcggatgaag gcagacctaa aactagatta acggttaaaa cgtcatcaga
    28621 ttgatcttct aaacgtgcta ggttagctaa agagaaagat aggatatttt ggtaggtttc
    28681 ctgcttgtat ctgctgatcc caaaacctag tgattctatc acgtagtcat cttttcctag
    28741 ctcgtgtata tccttcccaa aataatagct ttcagtagtg tttactaggc tatattcaga
    28801 aacgtttagt gatcgtttta atgaggaacc tatattagaa aaacgtcctg aattaatgat
    28861 atttttgttc aaaatacgag agggaagcga gagtggagaa acgtgtgtac tcatttcttt
    28921 tactgattta ttccctaagt caacggaaaa caataatggt tctttcatgg tatgacctct
    28981 ttctgtttgt gtttatggta tttcggtacg aaaatagttt acacctattt actaatagtt
    29041 gcaactgttt ataataaaaa aatgcaatca aaaaagtata atcaattaca ataaagtgta
    29101 agtaaaaagg tgtaaataaa cacttgcaaa tttatcttat tatgatatta taaaaacata
    29161 aattatatta aggagtgatc ctgatgcgat tgagaagaaa taagaattac actactatta
    29221 ttttagaaga ttttactgat caagaattac ttattgtcgg taaaacaaaa gcagatttta
    29281 tcgaaaaggg aaatgcttca atagtaaaag cttcaaaggt tgctgtcgaa aaatattttg
    29341 attctttaga agaaagtgct aaggaaattt ttaatggaga gaaagctggt agctatgagt
    29401 tagatggtat taaatttagc ttgatcccaa caactgaaaa tattattccg aagaaattat
    29461 attatatctt ttcatctgaa aaacatactt caatgaagcc ttgcattttt gattggcaat
    29521 ggttgattga tgctgtattg gatgtaaaat gttcaaaata caatattact ttggggtgtt
    29581 aattatgagt atgctagtta gaaaattacc attatctgta agagaatttc tattttactt
    29641 ggtctgctta atcgcatcag ggtttaagtt gatcgaacat atttttatct ttgcttattt
    29701 attagcaatg atcgcatttt tatctggcgg ttggttaatt atatatttgt taagctaatt
    29761 tttagatcaa aaataattaa taaattaaac gataaaataa ccgttggaaa atgtaattca
    29821 aggacaacac ctagcaatca gcttgtaatc aaattataaa ataacataat tcaataatta
    29881 atgcttttta aaggacaaat taagtgtatc aacataaacg tgtatatgta tatgtttatg
    29941 ttgatacttt ttttgtctta atttttttat tttttaaatt ctatttacta atgctgtgta
    30001 ggtagtgatc agaaaaaaca aaatagcgag gttaagctat ttattagttc atatattata
    30061 ttcagattaa tatgatccaa tattaatctg aaggtgaaac caatatattt ctatggattc
    30121 atgtaatatt atttagatgt tcctgctaat aaaataacaa aaataaaata ttcagctatg
    30181 ttataccatt ttacgataac attattctat ttgtaatatt aaaacattta aattttgaat
    30241 tatttttcta aattagatca atttatattc ttacaaaaca gtataaaaaa tgatttttat
    30301 atcgttattt atttatatta ttctcataaa aaacaatgaa atcgcatact tttatgaatg
    30361 gttaaatgta attttaatac ctataccaat taaaagagaa tttttcatat tttcacttaa
    30421 aaatttataa aaaaacaaga taattgatac ttcgattttg cgaacatttt gcctttccgc
    30481 ctaattcagc tttaacatca tgattatatc ggcaacaaat cgctttttta gataatttaa
    30541 gcatcaaata attatcctta atcagggtaa aaaataacga ataaatggat tttgggaata
    30601 ggtaaaaact agatcggttt ctgacgaaaa ataacatgaa aaaggaaata taaaataaca
    30661 tggatcgtgg gatgaataaa gaaattttac acataagggg cgtatggata taaagaaact
    30721 taacacgaaa caaaagt
//
LOCUS       DACENJ010000008        30251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.008, whole genome shotgun sequence.
ACCESSION   DACENJ010000008 DACENJ010000000
VERSION     DACENJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.008"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..965)
                     /locus_tag="IW748_000259"
     CDS             complement(<1..965)
                     /locus_tag="IW748_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1103195.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSVMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKG"
     gene            1045..1731
                     /locus_tag="IW748_000260"
     CDS             1045..1731
                     /locus_tag="IW748_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1103196.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(1932..2459)
                     /gene="msrA"
                     /locus_tag="IW748_000261"
     CDS             complement(1932..2459)
                     /gene="msrA"
                     /locus_tag="IW748_000261"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1103197.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(2543..3220)
                     /gene="pgmB"
                     /locus_tag="IW748_000262"
     CDS             complement(2543..3220)
                     /gene="pgmB"
                     /locus_tag="IW748_000262"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1103198.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(3217..4347)
                     /locus_tag="IW748_000263"
     CDS             complement(3217..4347)
                     /locus_tag="IW748_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1103199.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(4347..6530)
                     /locus_tag="IW748_000264"
     CDS             complement(4347..6530)
                     /locus_tag="IW748_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1103200.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(6527..7552)
                     /locus_tag="IW748_000265"
     CDS             complement(6527..7552)
                     /locus_tag="IW748_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1103201.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(7597..8424)
                     /locus_tag="IW748_000266"
     CDS             complement(7597..8424)
                     /locus_tag="IW748_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1103202.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(8421..9488)
                     /locus_tag="IW748_000267"
     CDS             complement(8421..9488)
                     /locus_tag="IW748_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1103203.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(9514..10350)
                     /locus_tag="IW748_000268"
     CDS             complement(9514..10350)
                     /locus_tag="IW748_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1103204.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(10347..11216)
                     /locus_tag="IW748_000269"
     CDS             complement(10347..11216)
                     /locus_tag="IW748_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1103205.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(11286..12569)
                     /locus_tag="IW748_000270"
     CDS             complement(11286..12569)
                     /locus_tag="IW748_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1103206.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(12583..14268)
                     /locus_tag="IW748_000271"
     CDS             complement(12583..14268)
                     /locus_tag="IW748_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1103207.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            14543..15568
                     /locus_tag="IW748_000272"
     CDS             14543..15568
                     /locus_tag="IW748_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1103208.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(16163..19816)
                     /gene="rpoC"
                     /locus_tag="IW748_000273"
     CDS             complement(16163..19816)
                     /gene="rpoC"
                     /locus_tag="IW748_000273"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1103209.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(19895..23512)
                     /gene="rpoB"
                     /locus_tag="IW748_000274"
     CDS             complement(19895..23512)
                     /gene="rpoB"
                     /locus_tag="IW748_000274"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1103210.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            24240..25208
                     /locus_tag="IW748_000275"
     CDS             24240..25208
                     /locus_tag="IW748_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1103211.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            25226..26131
                     /locus_tag="IW748_000276"
     CDS             25226..26131
                     /locus_tag="IW748_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103212.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            26128..26934
                     /locus_tag="IW748_000277"
     CDS             26128..26934
                     /locus_tag="IW748_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1103213.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            27091..28059
                     /locus_tag="IW748_000278"
     CDS             27091..28059
                     /locus_tag="IW748_000278"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1103214.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(28376..28900)
                     /locus_tag="IW748_000279"
     CDS             complement(28376..28900)
                     /locus_tag="IW748_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1103215.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            29187..>30251
                     /gene="scm"
                     /locus_tag="IW748_000280"
     CDS             29187..>30251
                     /gene="scm"
                     /locus_tag="IW748_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1103216.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         8495 a         6528 c         5701 g         9527 t
ORIGIN      
        1 cctttttctt tagcagattg aacgacttcc tgtaaaaatg tatgccctgc ccaatcaaag
       61 atgccagcac cattgctagt cgctcctccc actacccaat gtttctgatc cagaacataa
      121 caaaacgtct ccatttctgg atggaactga ggctgagtag taataaaacg aagtgctccg
      181 ctcgttccta cggtcaaagc ggcgatcgta tcaccaaaag cacctaaccc tagattagaa
      241 agcggcccgt ctgcccctcc caaaacaaat gatatatcat ttggcaaacc tagttctgct
      301 gctgtttttg atggtagacc tgttacttga taggtggaag gaaagacttg cggcaattgc
      361 tctattcgaa tgttcaagta gtcaagcact tcttctgccc attggaattc atggatatca
      421 aaatatccag ttcctgaagc tgttccgtag tcgctgatca gttcgccaaa caatctataa
      481 aagatatagt ctttgatacc gacaaaataa gcagtttttt tgaataattc aggttggctg
      541 ttttgcagcc acttgatttt gttcaatggt gtcattggat gaatcggcgt cccagtctgc
      601 cgataaaatt ctttgccaag cacggtatat ttcagttttt ctgcttcgtc cgctgctcgg
      661 ttatctgccc aagtgatcat tcgagataac ggtgttttct cttcatccaa caatatcagg
      721 ccatgcatca cagaggagaa ggagacaaat tgaatgattg tgtctgaagg caactgttta
      781 attacttctt ttataacggt gcagacacct tgaaagatca attcctgatc ttgttctgcc
      841 atcccttgtg tttcttggat caaagggtag cgaaaataag cggatgctgc ttcttgggct
      901 tcatctgtga aagcaactgc tttggtttgt gtggttccga tatctaatcc gattgaaacg
      961 ttcatttttt cacctcaaat tttatgtatg tacaaaaatt tagtttaagc ttgcgtatct
     1021 tggttttgaa aagggggaga tggcgtgtta ctttatttta tcattggttg ctgtatcgga
     1081 tcatttttat gtttaaccgc ccagcggatt ccgttaggtc actctattat ttatcctaga
     1141 tctcattgtg tcaaatgctg tcgttcgctt tcttggtatg aattgattcc gattctctcg
     1201 atcatcgtcc aacgatttcg ctgccgctat tgcaggtgtc ggttgccctt ctactatttg
     1261 ttggcagaag ccttatgtgg tggtcttttt gcctggttct ttactttttc ttccgcaaga
     1321 aatttttcga cgttcatatg gatgctttcg gctttgctat tttcgttgat ggatttattt
     1381 tacttcatgg tccattctca cacgctattc tgttcttggg caattttatg gattttttgg
     1441 ttgcaggccg gcgtcttcca atggcaaagt gtgctgttaa cgtttatcgt aggcggtgtg
     1501 tgccttcgct atggtcaatc ctatctcgga gctggcgata ctctgttgat gctttcttgg
     1561 agtggtggct tgtctctaga agagttgctt cagatcttgt ttcttgctag tctgtttgga
     1621 atatccgttt tttctgttta ctttatctgt cataaaaaaa agttggaaaa gctgcctttc
     1681 attccttttt tgagcagtgc gttgctgatt gtccttcatc ttaggtattg aaaaaaggtt
     1741 gtgacaaacg acttgtcaca acctctgata tatagtgttc tcttaccaaa tccttgtgac
     1801 ttggagctag gcgacaggtt cacaacctct ccactaaatg gtgttcaatc agctgaccct
     1861 cagtattagc tcaaaatgct ggttttacaa cctctgatat acggtgttcc ataagtagct
     1921 ttttcggctt cttatttctt gacgccccaa tgtcgacgaa ggaaacctgc acgtccagaa
     1981 ccttctcgat aaagattgta atggagcttg tttttcttgt aataatcctg atggtaatct
     2041 tctgcaggat aaaattcgct tcttggttcg atttttgtca cgattggctt atcgaatcgc
     2101 ccgctgtctt gcaatgcttg ttttgaacgc tctgcaatat ttcgttcttc ttcatcatta
     2161 tagtagatca ctggacgata agaatcaccg cgatcagcga actgtcctaa tgcgtcagtc
     2221 ggatcggttt gctgccaata gatttccacc aactgatcat aagaaataac ttctggatca
     2281 tattcgatct ctactgcttc tgtatgccct gttgtgcctg ttgttacttg ttcataagtt
     2341 ggatttggta catgcccgcc agtataaccg gatgtcacag aaatgatccc tggctgctct
     2401 tcaaatggtt tgaccataca ccaaaagcat ccgcctgcga aaactgcttt ttttgtcata
     2461 taaaatcccc cttttttctc atcatacctt gatttccaaa aggaggataa cgttttgctt
     2521 gtgacagaaa aaacggaaac tattatatac gtgatacggc ttctgataaa tgccaaaacg
     2581 aacgtacttg atcaaaaacg gttaaatcag gaatcacata ttcagcagaa ggaagattta
     2641 tgttttcacc tattcccaat acatgcatgt taccagccaa agctgcttga caaccagctt
     2701 ctgaatcttc aatgaccaag catgcatttg gcggtacatt caattgctgt gctcctttga
     2761 gaaaaacttc tggatcaggt tttgcttttg atacttgcgt gccgtcaata aggacatcaa
     2821 aataacttgt caaattcgtt ttttctaaaa tgaggcgtgc atttttgctt gctgatccca
     2881 atccgatctt gatgtttgtt tttttcaagt actcaagaac agctattgca ccaggcaaaa
     2941 cggatgtttc atccattttt tgaatcgctt gaagatatag attgtttttt tgctcagcaa
     3001 aggctgattt ctctttttct gagaatttct gttccatttt tccaaatgac agcaatcgct
     3061 ctagtgactc ggttctggat attcctttca gttgctcgtt ttctttctca gtgaatggaa
     3121 tggacagctg gttagctaaa accagccacg cttcgtaatg ataacgagct gtgtctacaa
     3181 gaacgccatc taaatcgaat agtgctgctt tgattttcat cgattacctc cttgaaaatg
     3241 aacgacttga agtaaagatg cgatgttttt agctgtgcga tgaagattat cagtggcttg
     3301 ctgtaaagct tcttgtaaag gcacagctgc aggaaggatc ggaaaaacgg ccgaaatgcc
     3361 aacatctgct gctttttcgc ttcctttacc aacacttccg caaatagcta tcacaggaac
     3421 gccctctggt actcttctag caatcccaac tggcgctttt ccttttagac tttgtccatc
     3481 tagacgtcct tcgcctacga ttacgaggtc tgcttgctgt acccgtttat cgaactccag
     3541 taaatccaat acgtagtcga tgccagagac tattttccct gaagcaaatg ctaacagtcc
     3601 tgcacccatt ccaccaccag cactactgcc cgatgcctcg attacttgag gggcaaatct
     3661 catataaaaa tcatgcatgg cttggtcagc cactgctaat tcattttctt tcaagccttt
     3721 ttgtggtccg aaaacaaaag aagcgccttc ttttccgcat aaaagattag taacatcagt
     3781 aatgacagtg attttggctt taatcagctc aataggcacg ttcgttttat caactgtagc
     3841 aattttacct agattttctc ccagtgcttc tactttttga ttatcttgat cgtaaaaagc
     3901 ataacctaga ccttctgcca tgccgatgcc gccatcgttg gtactacttc cgccaacgcc
     3961 gatgatgatg tgtcgaacac cttgttgcaa caattgtcgt atcagttctc ctactcctcg
     4021 cgtggataac ctaagtggct gttgttggtt tagtgggaca tctttcaatc cgacgatttc
     4081 tgccatttca aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc
     4141 aaatgcatta gtaacgactg ctgtttgccg cttcaacctt aaactgtctg ctaatgatga
     4201 aaccgttcct tctcctccat cgcctatagg tagcagatca tattcagcct gaacaaaaac
     4261 ttcttgaaaa cctgattgaa tggcttgtgc tgcctctaag gctgtcagac tttctttgaa
     4321 agaatctggc gcaatcagaa tacgcattat tttcccctcc gttccaagat cagttctttt
     4381 tccagctggt attggtctcc attgacaaaa ataggtaatg tttctttcgt ctcttttttt
     4441 attactattt gtttttttgt caaaacaaat agcaacctgt gatttttcca aataaaagga
     4501 aaagaaattt tttcccatgc tgaaggaagt cttggagaaa tagaaagagt tccattcgtt
     4561 actgtaatac ctgaaaaacc aaatataact gctagccata gtgctcctaa tgaagcagca
     4621 tgaattccgt catctgatga atgcggatct ggtcctaaat caatcaggca cgcttcttgg
     4681 aataaatgat acgcccattc catttcttgg atacggcaag cctctatggc gtgaatcgct
     4741 ttactcaacg atgagtcgtg gattgttttg ttttcatagt aatgcaaatt cttagaaacg
     4801 attggttgag aaaaatactc aggtaataag tataacagca tgacaagatc tgcttgcttg
     4861 ctgacttgga gttcatttac ttcgctacgg gcataatcaa gtaaaataga ctggctgcca
     4921 ggacgttctt tgtaagtggt caaatcaata actggcttac tgagatacgt gtcatcttgc
     4981 ggaatcagct gttctttgtt aggcactggc aaatataact tatctaaaaa attttgacat
     5041 tctttcaaaa aaagcggatc gccagcttga tatttaacag ctaaggccac attataatgc
     5101 gccagatagt tcgtatatgc attattgtcg atatattctg tgtattcatc tggaccaatg
     5161 actctgtgta tctctaaatg atcatcgact tttacagctc tgctcagcca aaaagccgct
     5221 gtttcatgaa tgagcgtttt tccctttgtt tctaaaaacg attgatcctc tgttgcttgg
     5281 acatactctt gaacggcata agcaaggtct gcactgatat gatgttctgc ggcagctgaa
     5341 gcaacttctt gtcttgttcc ggttttgata ttcaatgcag caaatttggg tgtctcttcc
     5401 tgaccagtgt gtgcgctttc ccatgggaaa agtgcacctt gatagtgata atctcttgct
     5461 ttttgccgtg cgccttctaa tcgatcataa cggtacagca gcaaattctt tgctacttca
     5521 ggaaactgat ataaaaagaa aggcaggata aaaatttccg tatcccagaa aacatgccct
     5581 ttataacctt cgccagttag tcccttcgcc cctacactaa cacttggatc attgcgggga
     5641 gtcataatct gtaaatggta agcagcgaag tccatcgcta actgatcgaa agaagaatcg
     5701 ccttggatcg ttatccgatg ctgatgccaa aaatctgtcc aataagacat cgactgctct
     5761 aaaatttctg tataactaac gcttttctgg gcttgggcaa tacttttctt aaacgggtct
     5821 gtttgctgaa gagaggtaga gagcacgatg atcttctcca gcgtaaagga cgtatttttt
     5881 ctgatctttt ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa tacacctgct
     5941 ttattgaatt tagctgcaat cccaatatca attttacttt ctgttgtctg gtaggatgca
     6001 ctgatcaaat cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa
     6061 ttggtttgct gtccatcgat acccgttttc acatctactt ggcagtcttc ggtcaacggc
     6121 ttgatgacca ctttcagggc tgcctgatgg agattatttt gatcagcaac acgacaagta
     6181 atgacttcca ctttttttcc ttgagcagtt tcaatgagcg catagcgttt aagctctccc
     6241 atttctaaat ctagtgcccg ctcgtatgca agaactttca tcgtctcaag agaaaaatgt
     6301 tcaccgtcga tttttatttc ccatctgatg acatctggga gattcactaa ctcggctgat
     6361 tctgctccta aaggtcggtt atatacacca gctgcaaaca ttccccttac ttgattccga
     6421 tagtcttcct cctggcttcc tctgatacca aaaaatccat ttccaatggt catctgactc
     6481 gcatacttat tgatatactc taaatcaaac tgtttttctt ctactttcat aaggtaataa
     6541 cttctcctgt ttctgccgaa cgatataaat tttccaccag attctggatg acatatcctt
     6601 gccagccatc tgcaatcatc tgagtctggt caccgagcgc ccgattcaca aaatgagcca
     6661 tacttagttc atgtgggtca ttaactggcg tggtcttttg taaggaaacc agctttccat
     6721 ctacatcttc gaatagttcg cctggataca gattagcacc tgcctgatcg ccacaaaaag
     6781 ccacattcag tttgtcttct ctgatattca aagcaaacga agtattcagt tggatgatct
     6841 tgccgttttc taattccagt gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat
     6901 cccatttccc gaatttccct gaattttttt tcgtaccaat tttttggaag ctgtacgccc
     6961 gaacgctttt tactttagga aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc
     7021 cataatcgat caaaggcccg cctccttgaa gttctttgtc gataaacgtt ccccagcctg
     7081 gaacaccaga ccgacgaagc gcattcgctt ctacgtaata aatatccccg agaacatcaa
     7141 gatgattctt gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa
     7201 tctgcccgtt tttatcggct gtctctgcca tctttcttgc ttgatcagct gtcatagctg
     7261 gtggtttctc gcacatcact gaagcattag cttcgagggc agccaataca tgttcgaagt
     7321 gaaagcgatt gggtgtacag acactcacga tatcgggaga aatctcattc agcatttgtg
     7381 taactgatgt atacgcatga gaaaatcctg tacgtcggca aaagtcttct gctcgctggc
     7441 tgactggatc aaccacagcg gccaattcta agacatctga tcttttttga taataagcag
     7501 catgtacttt ttctgctact tgtccagcgc ctatgattgc tactttcttc atttatttct
     7561 ctctcctttg cttatttact tattgttatt ctttctctat tctttctcta aatcatttaa
     7621 atgacgcgct gtttccagac agtttttcaa gtattgcaat gattcctgat aaattttcac
     7681 ttcattttct cctcgcaaac gacattcata taccatataa ccttcatact cattttgaag
     7741 tagcgtttga aaatggcgag taaaatcaat tgcgccagat cctggttggt agcgatgatt
     7801 atctgctaaa tggatatgac ccacatactc tttttgcgta gtcaatgctt ttgaaatatc
     7861 atcttcttca atattcatat gataaaaatc agcgatgatc tgcacatgtt tcaattgatt
     7921 agagtcaatg taatgacgcg catccgctaa caaattgatc atatggtctt ggtatcggtt
     7981 caaaggctcc aaataaataa ccgtacctgt ttcttcggct actttatcta aataacgaag
     8041 tgattcgctg actgctttat agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa
     8101 tcgataagtg aacattcccc aagctgctgg gataacgatt cctttgcctc caacctcgct
     8161 tagtgcacgc aaaatctcag caatttgacg tacaccatta agacgccgtt cttcgataaa
     8221 gtcaccgatc cacccatcgt agccgttaca agctgtcact actgggatcc ctgtttcttt
     8281 gattgcctgt ttgactgtgt cgatattttc tattaacaac ttaccgtcga tttcgtattc
     8341 ttcaaatcca cagtctttga taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc
     8401 ttgatcttgc gtaccgaatt tcattgttct cctcctcgga cttgaacgcc cattttgata
     8461 ctttcttctg ggtgctggtc tacataacgc atgtaagctt ctgcgctttc tgcaaatggc
     8521 acaactggat caatgatttc ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat
     8581 gtgtcctcga ttcgtttacg gttccatctt gggtaatctg ggtttggttc actagatgct
     8641 cgagcaaaga cgatttttgc attattgaaa tgtgcttcgc gaccaagatt aaacccagct
     8701 ggaaaaggtt tagcaaaggc aacataagaa atgatcccgc catagccgat ccctcttaaa
     8761 gaagcttgta atgcggcagc actgccactc gtttcaataa ctgaatctac tccacgttta
     8821 tctgtcaatt cctttagacg aaaaccgaca tctgctttca taggatctag cgtgtaatcg
     8881 gcaccgtatt tttctgctaa ttctctacgc tgcgctatgg gatcgatccc aacgatggta
     8941 gtagcacctg cttttttagc taattgaatc gcaatttgcc cgatagcgcc taaaccgaag
     9001 attgctacag catcacctgc tcgaacatgt gcttcccgga taccacctaa agcgaattga
     9061 gcaggatcgt aacatactgc atttttccat tgatccaacc gatcaagttt acgtagttta
     9121 taattatcaa caccgtttgt aatcagcgtt tcacgaatgg gtccataagt gcagactaac
     9181 tctccttttt gatactctgt caccaaactg cccgtttctt cgatcgtacc gacaatcata
     9241 tttcctaatt gaaattcgcc aaataccaca ccgcgttggc ttccttcttc tcttggcaag
     9301 aataggcgcc attcttctga aaattcttca tcgataaatg gacttaatcc tctaaagtcg
     9361 acaatttctg ttcctggttt tggagtagcg aaaattactc ggatacgtac ttcgttcttt
     9421 tcgatctcac ggtcttcgta atctttgaat ccggctattc ttggttctaa tgcaactaat
     9481 tttttcatct tttctttcct ctttcttttc tttttatcct tttacgccgc cttcgactaa
     9541 atcaccttta ataaatcgtt cagataacgc atacataatg atgactggta aagcggtcac
     9601 caatgaagcg gccatcattc gtccccaaat gtagtctggt gtactgaata atgtgttcaa
     9661 accgattggt aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa ataaataatc
     9721 attccacgaa accataaagc aataaacgaa gacagaaatg attccagaaa tagccaatgg
     9781 tataatgatc cgaataatga tctggatccg gctaagacca tcgatcattg ctgcttcttc
     9841 aatatcagtc ggaatcgtgt caaaataact ttttagcatg aatatcgcag taggaagtgt
     9901 ttgaacgacc atcgtgataa tcagtgctgt attggtatca tacaaaccta gaccggaaat
     9961 gattttaaat aatggaacaa cgatcaaaat accggaaaac atataaacgg tgtaaaagct
    10021 agcattgatt ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg ccccgctgat
    10081 cccaatcact actgcagtta cggaggctat caaggagatc actaaactat ttttgaaata
    10141 catgatgaat ggaaaaatct cagcattgaa aatatcctga taatgttcaa aagtccattc
    10201 tttcggaaaa atagtttgtg tcgttgcaat tgcttcttgt gaatccttga atgatgtcat
    10261 cagcatgatc aaaaatggaa acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa
    10321 taagattttc ctgctctttt tatttttcat ttcaagtcca ccttctttcg tgagagcaag
    10381 acgaccgctg caataatgat gaacaataga atcgatatag ctgctgcttt tccaagatca
    10441 ttgaacgcaa aggctgtctg atacaaataa acgcctaaga tattgacttt attcgtcagt
    10501 agatagacat ctgtaaacat atagaacatc cagatagatc gtaaaacaat cacagttgct
    10561 agtactggtt tgattgccgg cagtgtcacg atcttgaact tctgccaagc atttgcaccg
    10621 tccatttcag ctgcttcata aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa
    10681 ataaacgcat acggatagta tctccagata gcaaacaaaa cgaccataaa gaagctagca
    10741 actggctggt cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc tactaacaga
    10801 tcattgacga ttccataact attattgaac atatatttcc aagcaaaaat caaagaaata
    10861 gatggcgtaa cataacttaa taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg
    10921 cggttaagca aaatagctac ggctaatcct aaagctgtac tgcctgctac aactaagaat
    10981 gtgtaagcaa ctgttaaacc taaagaacga taaaattcac tatcacttaa aatttcaaga
    11041 taattgctta atccgataaa aacggcgtct gagttaggat tcagcggcat atcgaaaaaa
    11101 ctgatctgaa tattggaaag cattggaatc agcactaaaa cagctaataa aagcatgctt
    11161 ggtgctaaaa gtcccatggc taattttaag tcagaatgtt tccttggacg attcattgaa
    11221 acctccccct gataatctgc caatagctat ctactttttt cgaacaccat tggcagaact
    11281 tcttgttatt tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg
    11341 acagttactt ggttcactgc ttttccgatc acacctgagc ttgtgatatc tcccattttc
    11401 gtgaaattct tttccccgac tagtccaaag acttgtactt catcaaaagc actagcaatc
    11461 tcagatggca gctcgccaaa agacttgatg acttcatttt ctttgtaacc atccagttcg
    11521 actacttgtt tattcactgg ttgagcacca ccaggagaca tcaagaccca cttttccatg
    11581 tttttcggtt gagataagaa agctgtgaat gcttcagcag cctctttttg tttctcgtct
    11641 agaccagaag aaatcgtcaa tccagatact gtgccataga cagcttgatc tttttgtgtt
    11701 gggatagcaa aaccgatatt ttctgagttc cccgcttcat aaacagaagg caaaatgtaa
    11761 gttgaataga ttgccattgg tgctgttcca ttcatgaaag catctttgat ttctgtcaca
    11821 tcattagagc ctggcatcgt gtattgggat aagttttgat aaaacgacaa tgcttctctc
    11881 atcttttccg tatcgatcgt tacctcgcct tcatcatcca aaacatttgc tccatttgac
    11941 aaagcaaatt gagagaacgc ttgctcactc attgttcctt cagcagtagg catagcaatt
    12001 ccatattgtt tattctcttt atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa
    12061 ttttctggtt ctgaaaaacc agcttctgat aatttttctt tgtcatacca aataccttga
    12121 acccagccgc taattggtgc agcaatatag ctttttccat cttctgaacg aacaaggttt
    12181 ttggctccgt cataataatt atcttctcca gcttcctcga taacgttttg gactgccgtc
    12241 tgatcaatca gttcatcttt atccatcact ttagcaaaat cctgactgac ttccattact
    12301 tctggtaatt taccggaacg agctaacgta acgatttttg tattgaacgc tgattcttct
    12361 accggcactt gcttgatttt gatgtctgga ttttcttttt caaaatcagc aaccagttca
    12421 ttgatcacac ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt
    12481 tgtgattcgt tagagctttg actttcacca caagcagtaa gaagaaatcc agtagctagc
    12541 agacaggtag taacaataat ttttttcatt tttttccacc tcttatttta ttattttcag
    12601 ccacatatat tcatacggct ggaatatcaa ctttcgttct tgactgcaat ggataatttg
    12661 tccagaaaac aggtctaggt aatctccttt tggtaaaaga taacctgctg attgattgga
    12721 aaaattatgg ataactaaga ctttctcttc cttcgcttcg cgaataaaac tgataaaaga
    12781 atctgctgag tccagtaagt aaatcaaact attcggatgg aaggcttttt ctttttttcg
    12841 aatagatagc atctgggtca attcaccata tactgctgtt cttaatggat ctgtctgcaa
    12901 ctctttctca atatctgcta acaaaaattt ttgccgattg atttttcgat tctctcctgt
    12961 tttttctacg cctttgtaat catttcgact tcccagcaca ctttgaatat aaattgccgg
    13021 cacgcctggt aacgttacca aaaatgaatg agcagctaaa aagcgtttgc ttctccattc
    13081 cgtcgtatct gttggagaac ttaacgcatc tagatacgta gcgtttatct catatggact
    13141 ggtcgttcca tctgggtttt tcttgtagtt tactcgggca ccctctttct tcagctgttc
    13201 cactaattca aggatcgttt tttccggcag aatacctcgt accggattca atccgatacc
    13261 gtcatgggag gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt
    13321 taaccattct ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg
    13381 tagcgaaaac tgatagacca tctgtgcttc attctcgcca ttcccaaaat aactgatatt
    13441 atctttgtgt ggcacatttg tctcagtgat caataccgtt cctggagctg cttcatcagc
    13501 gattgcacgg aataattgga tgattttatg cgtttctggc aggtgtatac aagaagttcc
    13561 tagttctttc cataaaaatc ccacagcgtc caagcgaata aatgaagcgc ctttttctaa
    13621 gtaaaacaaa agaatatcga tcattttcaa taaaacttct ggatttttaa aattcaagtc
    13681 tacttgatct tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa
    13741 cgttgtaagt aacgggctgg tccttggtct agtcacgctg cttgtatctg tatccagtgg
    13801 caaatcaata aaaaaatctt tgtacgtatc attcaagttt aaatattcct gaaaccaagt
    13861 actttcggca gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat
    13921 ttcttctata tcttcccatg tgcccaaacg gtcgttgatt gcctgataat ttacaacgga
    13981 gaaaccatca tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc
    14041 tgccaaatac ttttgataaa atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa
    14101 aaattgatcc ccataagtaa tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc
    14161 ctggcctttc ttctgctgat aaacactgac tttttgttca atcatagagg cggttttctc
    14221 agacaaatct gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg gattcccctt
    14281 tcttttttgg gagcgttccc aacgatttga tcatattatg gaaaacgctt actgtcaata
    14341 gcattttttt aaataatttt gatatacaca aaaatacaaa taagaaagct tatgtatcaa
    14401 taattaatag cttcttaaaa aaatatggtc tctcatgatc atgcaagcta ttcctaaatt
    14461 ttcaaaatca tagacaaaaa agaatgtcta ttgtactatg gaattattct aaaacgctcc
    14521 caaaaaggag gtgcatataa aattgaaacc tactatttat gacattgcga aaaaagctaa
    14581 tgtctcaaaa tcaactgtct ccagagtatt aaataaccag ccaaatatct ctgataaagc
    14641 aaaagcaagt gtacttcaag caatcaaaga attgaattat gaaccgagca aaattgcaag
    14701 aggactgtca ggaaatggtt ttgacgcgat tctagcatta tctcatcgta cttctcatac
    14761 gacctatggc aatccttttt tttcagatgt tattcaatcc atttctgccg tttctgaaca
    14821 aaataacttc gacttgattc tacaaacggc aaaaacacca gaagaagagc tagaaaaatg
    14881 cgcgaaaaag atccaagaaa aaatgatccg aggaattatt attttaagct ctacgatgga
    14941 tgaaacactt ctttttgaat tggatcaata caatattcct attgtggtaa tcggaaaagt
    15001 cggaaacgac tacacgaaca tctactcagt ggacacggat aatttccagg atagttacga
    15061 tttggtggaa acactgatca agaatggaca ccgtcatatc ggctgtctcc atgctccgac
    15121 agaagtttat gtatccaatg atcgtctcaa tggttaccgt caatgcttat ttgatcacca
    15181 cctagatatc cgtaatgaat ggattataga cggcggcttt cgcttagaag atagcatggc
    15241 agcaatgaat gatttaatga agctacgaga aagaccaact gcgatttttt ctaccgatgc
    15301 attaaaatca ttgagtattt acaaagtggc agaacaatct ggaaagcaga tacctgatga
    15361 tctttcgatc gcaggattca gtgatccctt attgtcctcc ctattttcgc cacagttgac
    15421 tcggatagac attccgacaa atcagttagg aatcaaagcc acagaacttt tattccaact
    15481 aatccaccac caataccctg aagaaaaaag aatcattctt tcaaccgaga ttactctcac
    15541 agattcgatc caacaacaag aaatgtagaa cagaattata aaagaacaga aacactgagg
    15601 ataagtcagt tactctgttc ttttttcaat cactcatccc tcgttacccc ctcccggtat
    15661 tttagcgaat ttttcttatt ttccatctgt tttagatagc tgtggataac tataatgttt
    15721 gtgttaaact attttttcca cagggaagaa aaaactattt ttaaccacgt tgtgtataag
    15781 tttttatagt tcgttttttt aaacacattt acccttattt caatccatgt tatttgggga
    15841 tacttatctc taaaacataa atagaataac agacatgtct actggttttg ttttattcga
    15901 ggaattattc aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat
    15961 ttattttgca ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga
    16021 tgatcgttcc gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt
    16081 tcttctagtt agacaaatta ttttagacaa accaaaaaag aggttgtgac aataatttgt
    16141 cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca
    16201 tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg
    16261 gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta cctataataa
    16321 cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga
    16381 cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt
    16441 ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat
    16501 tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat
    16561 cacctggatc cattacacga actttacgca gcatttgacg aaccattact tcgatatgct
    16621 tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt
    16681 tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg
    16741 tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag
    16801 gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg
    16861 caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt
    16921 tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc
    16981 cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg
    17041 attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt
    17101 tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga
    17161 ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt
    17221 ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac
    17281 gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga
    17341 tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt
    17401 caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc
    17461 ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt
    17521 tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc
    17581 cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga
    17641 ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat
    17701 tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt
    17761 gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca
    17821 gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta
    17881 agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt ttgaagactt
    17941 ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga
    18001 tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca
    18061 agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga
    18121 tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt
    18181 cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc
    18241 atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt
    18301 cttccattgt caagtagtag ttacctagaa ccatatcttg agaaggagta acaactggtt
    18361 taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt
    18421 gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat
    18481 aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga
    18541 atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct
    18601 tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg
    18661 catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga
    18721 ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa
    18781 caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac
    18841 gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag
    18901 taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac
    18961 gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt
    19021 tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac
    19081 cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca
    19141 tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg
    19201 cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg
    19261 catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga
    19321 attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt
    19381 tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt
    19441 ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt
    19501 accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt
    19561 tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt
    19621 tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca
    19681 acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac
    19741 cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt
    19801 tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc
    19861 tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt
    19921 caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta
    19981 attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt
    20041 cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca
    20101 tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg
    20161 gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt
    20221 catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg
    20281 cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt
    20341 gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt
    20401 gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg
    20461 tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt
    20521 cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact
    20581 gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca
    20641 cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt
    20701 cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc
    20761 cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg
    20821 acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc
    20881 catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat
    20941 gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa
    21001 gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt
    21061 ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg
    21121 tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta
    21181 ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac
    21241 caactaaaac gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca
    21301 gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg
    21361 ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc
    21421 ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt ttacataaca
    21481 aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt
    21541 gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg
    21601 agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg
    21661 atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag
    21721 ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta
    21781 cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg
    21841 tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat
    21901 aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca
    21961 tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca
    22021 atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca agagggtttg
    22081 tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa
    22141 ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc
    22201 tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt
    22261 caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac
    22321 cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt
    22381 ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg
    22441 gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg
    22501 gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt
    22561 ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg
    22621 tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt
    22681 aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt
    22741 tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca
    22801 aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc
    22861 gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg
    22921 cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt
    22981 cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga
    23041 atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta
    23101 actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta
    23161 atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca
    23221 aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta
    23281 ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga ttgccattga
    23341 agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt
    23401 ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg
    23461 cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca
    23521 cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat
    23581 attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat
    23641 agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc
    23701 cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata
    23761 tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa
    23821 cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga
    23881 cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc
    23941 aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag
    24001 gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa
    24061 agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa
    24121 aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc
    24181 catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa
    24241 tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg
    24301 cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac
    24361 ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag
    24421 aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag
    24481 acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa
    24541 tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca
    24601 cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca
    24661 atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata
    24721 tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga
    24781 tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga
    24841 cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg
    24901 gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg
    24961 caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc
    25021 tagcaacagt aggaatcgac tacgaaagct tgggcaaaca aacagggatc atggcagcaa
    25081 aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac
    25141 tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag
    25201 ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc
    25261 atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt
    25321 ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt
    25381 aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt tagggggtgc
    25441 ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc
    25501 agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa
    25561 catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa
    25621 agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt
    25681 tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat
    25741 gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa
    25801 tggatgcatt gctctttctg gctccttact tgcgcaaaac aacggctttg ctgatttaaa
    25861 ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt
    25921 acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg
    25981 ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc
    26041 tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc
    26101 tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat
    26161 catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc
    26221 ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg
    26281 ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa
    26341 aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat
    26401 cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa
    26461 cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa
    26521 gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg
    26581 ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa
    26641 ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg
    26701 gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac
    26761 atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc
    26821 gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc
    26881 aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa
    26941 agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt
    27001 tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg
    27061 atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta
    27121 ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc
    27181 cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac
    27241 gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa
    27301 gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc tgaatctacg
    27361 atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct
    27421 gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga
    27481 ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat
    27541 acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct
    27601 tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct
    27661 gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt
    27721 aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc
    27781 tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa
    27841 ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc
    27901 gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa
    27961 gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct
    28021 tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga
    28081 cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc tccttattct
    28141 atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat
    28201 ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc
    28261 aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa
    28321 aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat
    28381 ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat
    28441 caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta
    28501 cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc
    28561 ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca
    28621 gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt
    28681 cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc
    28741 gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg
    28801 gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg
    28861 cccgatccac actctcgcat agcatatcta aataatccat ttattttcct cctttttatt
    28921 tatcctactt actttttcct aattctagga tacctactta tatgttacta cacaccctta
    28981 ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt
    29041 gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat
    29101 aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa
    29161 tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg
    29221 ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta
    29281 aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta
    29341 gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata
    29401 tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat
    29461 tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac
    29521 ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc
    29581 aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt
    29641 ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg
    29701 tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc
    29761 cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg
    29821 taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg
    29881 atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa
    29941 tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt
    30001 ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc
    30061 ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa
    30121 atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca
    30181 atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct
    30241 cagattcatc a
//
LOCUS       DACENJ010000009        29926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.009, whole genome shotgun sequence.
ACCESSION   DACENJ010000009 DACENJ010000000
VERSION     DACENJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.009"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..600
                     /locus_tag="IW748_000281"
     CDS             <1..600
                     /locus_tag="IW748_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103217.1"
                     /translation="ADAEFTLTDEKGVKEVKKTDATGLATFNIIANKTYNLEETKVPT
                     GYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQGKALANAEFTLTDDKGV
                     KVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEGITVADDGQTFEYTVINH
                     LVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            649..2484
                     /locus_tag="IW748_000282"
     CDS             649..2484
                     /locus_tag="IW748_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1103218.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDIPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            2559..3416
                     /locus_tag="IW748_000283"
     CDS             2559..3416
                     /locus_tag="IW748_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1103219.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            3406..4485
                     /locus_tag="IW748_000284"
     CDS             3406..4485
                     /locus_tag="IW748_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1103220.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGESFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSESVSDKPETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVLGALASLKVLILLIKEFRKA"
     gene            4824..5624
                     /locus_tag="IW748_000285"
     CDS             4824..5624
                     /locus_tag="IW748_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103221.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            5712..6518
                     /locus_tag="IW748_000286"
     CDS             5712..6518
                     /locus_tag="IW748_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103222.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            6750..7619
                     /locus_tag="IW748_000287"
     CDS             6750..7619
                     /locus_tag="IW748_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1103223.1"
                     /translation="MEKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            7639..7986
                     /locus_tag="IW748_000288"
     CDS             7639..7986
                     /locus_tag="IW748_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103224.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            7999..9261
                     /locus_tag="IW748_000289"
     CDS             7999..9261
                     /locus_tag="IW748_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103225.1"
                     /translation="MIKKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALIVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNAQN
                     FFAKLHLVDISILVLEAGAVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRHKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETEKATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            9341..10717
                     /locus_tag="IW748_000290"
     CDS             9341..10717
                     /locus_tag="IW748_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ1103226.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNKKLNGKFSVNMLNTKAKD
                     PTDAILGIKPDGTIAHFCISEMPHLLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGVQKITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKHVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLSTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGN
                     ADPEPQTSNNTNNKQPITSKTKPKTTDPKVSVNVTITTDNSEKKQVNGTTSPIMQKML
                     KEKKQVHK"
     gene            10714..11343
                     /locus_tag="IW748_000291"
     CDS             10714..11343
                     /locus_tag="IW748_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103227.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKKDKKAVLTPKQMLDHGI
                     LGVKVLEMNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRIQINKFEKKVRK
                     FNDLKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            11362..11841
                     /locus_tag="IW748_000292"
     CDS             11362..11841
                     /locus_tag="IW748_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103228.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDENSVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            12343..12396
                     /locus_tag="IW748_000293"
                     /pseudo
     CDS             12343..12396
                     /locus_tag="IW748_000293"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12400..12999
                     /locus_tag="IW748_000294"
     CDS             12400..12999
                     /locus_tag="IW748_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1103229.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFILLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            13015..13491
                     /locus_tag="IW748_000295"
     CDS             13015..13491
                     /locus_tag="IW748_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103230.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            13508..14410
                     /locus_tag="IW748_000296"
     CDS             13508..14410
                     /locus_tag="IW748_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103231.1"
                     /translation="MLLTLLGYLCLILAFFGTIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            14483..14674
                     /locus_tag="IW748_000297"
     CDS             14483..14674
                     /locus_tag="IW748_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103232.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            14838..15425
                     /locus_tag="IW748_000298"
     CDS             14838..15425
                     /locus_tag="IW748_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103233.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            15425..16324
                     /locus_tag="IW748_000299"
     CDS             15425..16324
                     /locus_tag="IW748_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAQ1103234.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVSDATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            16324..17238
                     /locus_tag="IW748_000300"
     CDS             16324..17238
                     /locus_tag="IW748_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103235.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELEA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            17290..17505
                     /locus_tag="IW748_000301"
     CDS             17290..17505
                     /locus_tag="IW748_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103236.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NPVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            17695..17949
                     /locus_tag="IW748_000302"
     CDS             17695..17949
                     /locus_tag="IW748_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAQ1103237.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            18127..19170
                     /locus_tag="IW748_000303"
     CDS             18127..19170
                     /locus_tag="IW748_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAQ1103238.1"
                     /translation="MKKILVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTIDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNIISRQGWKIEGTGWYAMPNS
                     APNPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPRAN
                     DNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAGTYE
                     KSFNVTGMAVAVR"
     gene            19345..19575
                     /locus_tag="IW748_000304"
     CDS             19345..19575
                     /locus_tag="IW748_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103239.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            19578..19835
                     /locus_tag="IW748_000305"
     CDS             19578..19835
                     /locus_tag="IW748_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103240.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            20052..20927
                     /locus_tag="IW748_000306"
     CDS             20052..20927
                     /locus_tag="IW748_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103241.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKEE
                     ITKMKPVIKILDNKE"
     gene            20937..21731
                     /locus_tag="IW748_000307"
     CDS             20937..21731
                     /locus_tag="IW748_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103242.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMDKYRV"
     gene            21760..22878
                     /locus_tag="IW748_000308"
     CDS             21760..22878
                     /locus_tag="IW748_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103243.1"
                     /translation="MGNNSIYTPEMQNKIKRLVRLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKVKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            22911..23231
                     /locus_tag="IW748_000309"
     CDS             22911..23231
                     /locus_tag="IW748_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103244.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            23361..24044
                     /locus_tag="IW748_000310"
     CDS             23361..24044
                     /locus_tag="IW748_000310"
                     /inference="COORDINATES: protein motif:HMM:NF013235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1103245.1"
                     /translation="MGKNDFEVILMSLSVQYHNTMNNIAFKKFSNVEIDVFFAICSQL
                     EEKGSDEVSLSFRQLKELSNLNMMTDSSLVKVLRSLYKKMATLYFYRLSEDGSETCFV
                     IFKEYSIDTSKSNIAIKTNAEYMYLLNDLTSNFTIFEMKEFTSLRSKYSKSIYCLLKQ
                     SNLGKQVINIDEFRKLLAVPKSYKMTNIDKKILEPVCEELSIYFDELSITKVKTGKSI
                     VAFEFTWNK"
     gene            24270..24788
                     /locus_tag="IW748_000311"
                     /pseudo
     CDS             24270..24788
                     /locus_tag="IW748_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
     gene            25778..27640
                     /gene="ltrA"
                     /locus_tag="IW748_000312"
     CDS             25778..27640
                     /gene="ltrA"
                     /locus_tag="IW748_000312"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1103246.1"
                     /translation="MENVSASHSIRHMEYYGMIDSFDNLYKESSDNHVFNNLMEIVTS
                     SNNILLAYRNVKGNSGSVSPGVDNKNIDDLKEIPNADFIKIVQTKFSEYKPQPVKRVE
                     IPKPNGKTRPLGIPTIWDRIVQQCLLQVLEPIMEAKFHDKNYGFRPNRSAHHAFAQAV
                     RLAQVSKLTFVVDIDIEGFFDNVNHSKLIKQLWTLGVQDKWLLGVIRAMLKAPIIHKD
                     GRIEHPKKGTPQGGILSPLLANVVLNELDWWISSQWETHPTRHNYDWYHAEKGYWNKG
                     NKYRALRGSKLKEIYIVRYADDFKIFCRKRSDADNIFIAAKSWLKERLKLDISIEKSK
                     VVNLKKQKSEFLGFTMKLVRKRKTFVIETHMCHKAMKKVSDNLAKQIKVIQHTPTKEE
                     LQKQLVKYNSMVIGIHQYYKKATHISVDCPKINQRIMIIIKNRLDVKKNGHPPSDFVK
                     KNYACSKQLRWINGYPMIPLGFVKHSHTSLPKMGICKYTPEGRVLIHKYLTFPIDIIN
                     QLMNMNNPDESIEFRDNRVSKYSSQRGRCRVTKEFLELADIYCHRIIPKNLGGKDNFQ
                     NLVIIHRNIHQLIHENDKARATLLVMEMRITGTQLEIINQFRKKAKNCELSLNL"
     gene            27722..29017
                     /locus_tag="IW748_000313"
     CDS             27722..29017
                     /locus_tag="IW748_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1103247.1"
                     /translation="MEIVSKDYLSLSSVECIERNLDPMTTPLIVMSRSDNTGSGLTLA
                     SSPTAKKKYHITNVSRANDLPYPLPKELYIVPPRMNLYIKKNMEVNQIFRKYVADEDL
                     LIYSIDESILKVTKSLNLFTSSETRSERRRELAKIIKDEIEKETGLIATVGIGDNPLL
                     AKLALDNEAKSSKEFIAEWTYETVPKKIWKINEMTDFWGIGSRMKKRLNHMGIMSIED
                     LANWNPYTIKSQLGVIGLQLYFHANGIDRTDIAIPAEPTKEKSYGNSQVLPKDYTRRE
                     EVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKIPITDNTKELQ
                     NYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEENLDRIIDKIRQ
                     KYGFTAIIRASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            29010..29357
                     /locus_tag="IW748_000314"
     CDS             29010..29357
                     /locus_tag="IW748_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103248.1"
                     /translation="MIEYNDRGRLKWVGFYLSDHTERINSDNVQRANHNSAKEQMTTE
                     EIAKVLKTAVIKNRPVCIQKEERNAEGYYPDDIVGKIIGSDELGVFVEEEKVHYDEIR
                     NVSFAELVKWSQI"
BASE COUNT        11337 a         4153 c         6068 g         8368 t
ORIGIN      
        1 gcagatgcag aattcacctt aacggatgaa aaaggtgtaa aagaagttaa aaagacggat
       61 gcaactggtc tggctacgtt caacattatt gcgaacaaaa cttataactt agaagaaaca
      121 aaagtaccaa caggttacac aggcgcattc aaacaagaag gaattaccgt caaagatgat
      181 ggacaagtct ttgaatatac tgctaaaaac actttgaaaa aaggtgtgat caaagttcat
      241 aaagtggatg atcaagggaa agcattagca aacgcagaat tcactttgac agacgacaaa
      301 ggtgtaaaag ttgtacaaaa aacggacagc aatggcttgg caacatttaa tattgttgcc
      361 aacaaaacgt acaaaatcga agaaacacaa aaccctaatg gttatacagg gaaatggtct
      421 aaagaaggca tcaccgttgc tgacgatgga caaacctttg aatatacagt aattaatcac
      481 cttgtacctg aaacagtaag aacaggcgga atcggtacat cagccttcct tcttgtattt
      541 ctaattgcag gattgagtat ggtgctactg tattatcgag aacagcacaa agcaaaataa
      601 aaaagtgtta ctggaaaaga agtaaatatg aaaaaagaag gctgacgtat gaaatcaaaa
      661 aaaatttttt caaatttaat gctatttggt actttgctag gagtgggatt gggcgcaagc
      721 tcaatctctt ctagccttgg actagggggg caagtagcat atgcacaaca aaactataat
      781 tatcaaggaa ctggaacgat tcatatccat aaagtaacaa atactaatgg ggatgggaac
      841 cctgtctcag atattgataa caaagggaaa gaaatagctt taccaaatgg tgtaaaacct
      901 cttgaaggcg ttgagttctc ttatgtgaaa cttactaaag atgaagctaa atccttggga
      961 tggacggata gtgatactac aaaaggtcaa catattgatg atgctaaagc acgattatta
     1021 ctaaacaaaa aatacagtga agtaagtaga atgaagactg attcacaagg gaatatagac
     1081 atacctatac aagtaaatac agacgattta acagataact tgtatttgat tgttgaaaca
     1141 ggaacagtca aaggtgtagt tgaagaagcg tatcctatga ttgttaacgt acctgaacta
     1201 aacggatcag atgatcaata ctttaattat gatgtacatg tctatcctaa aaactatttc
     1261 caattaaact cagcagagct tgataaaaaa gacgaagatg gaaaggctct tgccggaact
     1321 gtttttgaat tgcgaaaaga tgatggatct gatcaggttg ttggaactta taaaacagac
     1381 agcacaggta aattaactgt agatggtttg gaattaggtg actattattt cctagaaacc
     1441 gatttgggaa cagatgctaa taaaacaacc tatttactag atggtaaaaa attcaaattt
     1501 agcgttaaac gtggggagac tgctaaagta gttcttgatg ttaccaacta caaacgacct
     1561 gtaattacta aggtaattga taaagaaacc gttgatactg gcgaagatgt tcaatataca
     1621 ttgaaaccat caattcctgg taatattggt aattacgaaa aatatgttgt caaagatgtt
     1681 ctaggtgcag agttaactta tgattcatca gaagtgatcg aaggaatgac tttagatact
     1741 gattacagta tcgaacgtga cggacaaacg acaaactatg tctttactga atcaggtatg
     1801 gataagcttc agaaacaatt tgatgcaggg gatcgcttct ttacaatttt gatgaacgct
     1861 catactaata gtaaagctaa aatggataca ccacttaaga atgatgtttc aacgatctac
     1921 aacaatggat atgaacccga agacgaaaca ccagttgatc acgcttatac aatcattggt
     1981 gatcaccaat tcattaaaac agatggagat acaagcaaag cattgagtgg agcaaaattt
     2041 gtagtgaaga acaaaattga tccgaataaa gttacttacc taaaacaaga tccaacaacg
     2101 aaagctgtta cttggattgc agataaatct aaagctactg aattcgtttc cggatcagat
     2161 ggtaagttcc aaattgaagg cttgcaatat acctatgatg tagattttga taaacaaggt
     2221 aatccaacaa tcacagataa aaaaatcaat gattatgctc ttgaagaagt tgttgctcct
     2281 tcaggatata caccaactag aaatgatatt ccatttgtgg tatcgaatga tgatgcttct
     2341 agcgaagtaa cagacatcaa gaaccttaaa acacctgaaa cagttcgtac aggtggaatt
     2401 ggaacagtag tatttctagt ggttgcaggt gtggcaggaa cagggctata tgttgtctac
     2461 aaacgagaac aacacaaagc ttaatgaaat aaaattccct agagtgtttt tggctctagg
     2521 gtttcttttt aaaaagtgtg tgaaaagtag gtatgagcat gaaaaagaag aatatcaagt
     2581 tgatcatctt aaaaattggg atgttgctat gtttaggagt aggtctatat cctgtaatta
     2641 ctcaggctta tacctactac caaactcaac aaacactaaa ctcctataag gaaaaatatg
     2701 atgaagcttc tgaaaaggag aatgaagaag tcttcaagag atattcagaa gttaatagga
     2761 aacgaaaaga cacagaacaa acctcagtca cgattcctga agataataac aatattccgt
     2821 caaatggaaa tcttcagaag gataaattag gtgaattagt gggtgaaatt cttataccaa
     2881 agattaatgt ggatttgcca atttatgatg gtgtaaacga ttttcaacta tcagcaggag
     2941 caggaatatt aaccggaact gattatccaa ctggtgggat tggaaaccat tctgttatat
     3001 cagctcattc aggcattcct aataaaaaat attttacaga tttagagttg ctcaaacttg
     3061 gtgataaatt ttttattgat atttcaggga aaaagcttgc ttatcaagta gatcggataa
     3121 aaacaatcct accaaccgaa tttgaggatt tgcttcctgt ggcaggaaaa gattatgtaa
     3181 cacttatgac atgtacacct gttggagata attctcatag gttactcgtt agaggggtta
     3241 gcatacctta ccaaaaaagt attgttgaga aagaaaagaa gaaagaagat ttaaaggtaa
     3301 aggcgcagat gaactggtat cctatcgtag ttgtaggggt tttattaata atagctgtga
     3361 tagtcattat taaaaaaata gtagcaaaga gacgaggaat gaaaaatgaa gaataaaata
     3421 ctactttttt tgactcttat ttgcactttg gttttaacaa tttatcctgc aaatcagctc
     3481 tatcatcaat tcaaaacaaa agagaatgtg gcggaaatcc aagagaaaat tgagaaaaaa
     3541 caaactcctt caaaagagga agtggcaaaa aaagtcgaac aaataaaatc gaacagcggt
     3601 aaccaaggat tagacaatac tcagaaaaca gacggcgaat catttgatat tggtgaagct
     3661 gaaggaaagg tcaatcctta cacaggagat gttttttcaa aaaatcaaac gaaagatttt
     3721 gaaccatcta cctattcagg ggcggttggt tatgtctata ttccaaaaat aaatgtgcta
     3781 gaacttttgt ttctaggcgc ttcacaggag catttatata gaggtgtggc acagatgagt
     3841 gctactagtc tacctttatc aggtaaaggt catcaaacag taatagcagg tcatcgtggc
     3901 ggttatacgt caccacaatt tctttatgtt gatcaattag tagccggaga ccacatttat
     3961 atagattatc ttggtgaaaa agcagtttat acggttttcc gaacagacgt tattagaaat
     4021 tacggtcaga attcaacact gactgatatt cctgatttag atatgttaac actttatact
     4081 tgtcatccat atcctcagaa ctacgatcgt ttattagtta gaagtctaag agatgaaaaa
     4141 gcaaaataca tcacgccaca agtaacagtt ggcaataccg catacgctga tgccattgat
     4201 agtgaatccg tttcagataa gccagaaact gttacatctt ctaatgattt atttgaggat
     4261 gttgatcgtg ccaacaagga attagatgga aataatgatc ttaatattcc ggaacaaggt
     4321 agcgtagaga tgcaagacag aacgtttgct gaaaaggcaa gttcaatatt tacaggaatt
     4381 accaataaac atcagaaaat gcttttagtc atttggactg tattaggagc attagcaagt
     4441 ctaaaggttc ttattctttt aatcaaagag tttagaaaag cataatgaag attataaaaa
     4501 ttgatagagt acagtaaaaa gggattgata ctattattga tctctttttt attttgaata
     4561 gagctagatt cacttttaaa taaaatcaaa actgatctta attccaaaga aaaagtcaag
     4621 aaaaaaatac aacaaatttt tttatcagaa tatttatcag aatattatta gaaaagataa
     4681 actcaaaagc ttaatttaat agggttattg gttgtattta gaaataattt atggaaaagc
     4741 tataaagcaa tagataagat gtagaaaatc ttatttgtga atgaaaaaca agtcatcgta
     4801 aaatattaat aggaggtgta gtaatggtgg aaatatcaaa aacagcttta gactcattag
     4861 ctaaactatt tagtagcagg tttgatggtg cagaatttgt tgaatatcta agccaaaacg
     4921 aaagtggaaa aactctgatc gaaacactga aaaaagacta tttgaatgaa aatgactttc
     4981 cgacatggac gaatattgaa gatgctacat gggatgtagt atgtacgata tattctcctt
     5041 gggtaacgat tgctgatttt gttttagata aaattggagg ggatacatac cctgatgcca
     5101 aatctcttgc tacaagtgac tttaaaacgt tctatggtta tctaataggt ctaataggca
     5161 atggaataga tttgaaagtt acgccaaaac aaacacctga agaaattgaa aaagaaaaag
     5221 gagaggttca aatggcttat gcaaatgaca gagaaaaagt aaaattattg gaaggtaaat
     5281 tatctgaaga agaacttgga aaaatggtgc aagaaatcaa tgaagacagc aactattttc
     5341 ctgaattgaa ggtatatcaa aatgacgaag atttctttaa tgaaatgttt agtaatagtc
     5401 catgggaagc agtgagtaaa acaaatttag gagaatggaa ctttcaagat gatctagttg
     5461 catttagcaa tgatgggtat ttacgatcat attcagaaag tgagtataga gctgaattaa
     5521 aaacgtatga aacggatatt ctgatgagct atttaaatct attagagagt ggtcagggag
     5581 agttaagaga taaagttgaa gaactattaa aagaagagct atagggatta tttcaacata
     5641 aacacaagca gaaaactacc aaacaaaacg taccatgcaa aaagccggtt aaaccggctt
     5701 tttgctctat tatgagagga gatcaaacca tgagtgcaac aaccgatatg gctttagaag
     5761 cagtaataaa agataataca ggaaatgtcg aaaatgtaga agactttagc gatattgcaa
     5821 tcaatctttc agcatggatt tcagctgtag gagaagaaaa tatcgaattt gagatcaaga
     5881 atatggatga cttaaaagca aaatttcctt ttattgcatc gaaactaagt caagttgccg
     5941 aggataaatc gtggaatgaa ttagttagtt tttccgaaaa aatttatgat attacgcaag
     6001 aagatgagtg tgtttcatgg gaaatgaata atcaatacaa gaaaattgac gctttcgtca
     6061 aatcacgtat agaaggaaca gcttttgagt ttgaaggtat agataccgca gataattggt
     6121 taaataaaga atcctttatt aaaagtatag cggatgatct tgaaaaacaa tggtctaagg
     6181 aagactatgc agagtaccta aaaacagaaa gagatacaga ggtgaaagaa aaactcactg
     6241 aggtatgttt ggatgaacat attgatccca atatcgtaaa acaagaagac tatgaagtgg
     6301 ttattgataa agttcctact gattttgagg aagttgtaaa gtatatagtt gaagaagcat
     6361 tatcgcaata ctcttcagat gaggaagtgg acgaagaaac tgttcgtacc agagtttggc
     6421 aactggtttt tgatgacaaa gattataaat atttgagtga tcaaaactcg gttaattgga
     6481 gtgtacgatt attaattgat caaaatgata ttgaataaaa tagagctgaa aagctctatt
     6541 ttttttgata aaaaaactag ctattttatt aaaaataatt tggttcaaaa tttatttttt
     6601 taacttgata attttttgcc atcctttttt aaattggcta tatatcaatg tttgtagtgg
     6661 gaataaataa aaatacattt gacaaaattt acaatatagt ttattatata tttatcttat
     6721 tatgataaca caataaaagg aatggtggta tggaaaagaa gaagtttagc cgatcgttgt
     6781 taggaatttt ggttttagtt gcggttggtc ttagtggcaa gggttacttt cagaataaca
     6841 gtactcagga acatgatttt cagatcaaaa gtagtgttca ggtaggtaat aaagcagaca
     6901 gtgaatcaaa agaattttta gggatacgtt atgatccaaa caaatatcca aataatatcg
     6961 taaacattaa tgatgggaaa tatacactga caaaagaaga ccatgaagca ttatcagaaa
     7021 aaggcagtca aaccttttgg gtaaattatg cagatttaga tcaacttgga agagcaggag
     7081 atgttgaagc gttgatcacc tatgatgctg taaattctca ctcatctaaa aatgtggaac
     7141 gtccatcttt caaaagttct gtccatattg caggagaata caaggatgga gtatttgatc
     7201 aagagagaga tacttgggtc ggcaaagatt caaacaatga aatcactcag ctagaaggct
     7261 ataaaggtta tctctataac aaaagtcatt cattagcatg gtcgctagga ggagatatgg
     7321 aggtacacaa tttaacactt ggaacgagag cgcaaaatgt tgggaaaaat gatcagaatg
     7381 gagggatggc ttactcagaa acgattgtac gtgatgcaat ctataaaaat aagaatgaaa
     7441 aagtattgta taaagttgat ccaatttata aagggaatga attagttcct cgtggttctc
     7501 atgtcgaagc tttctcagtt aacgataatg gaaaaacggt aaaccttaat gtttatgtat
     7561 ttaacaatca aaaaggaatc aacatcaact atcaaaatgg tacttgggta agagattaga
     7621 aattgagaag gtgaagaatt ggaaacaata agcatcaatg gattagataa atcagttgca
     7681 aatcgaataa aaaaaggaaa tggctactat cttctgaaga agggtaaata ttcagttgct
     7741 agagagttag aaacaatgag tatcgtgtat gtagctaaga atcattttga cgtagaacag
     7801 gctatttttg atccagattc tgaaataggg aaatgtgttg aattatcaga gaaaactgtt
     7861 agtacaaaag gggcgatgat cattgagaga gaaatgttag acgaaatcag agtaatcatt
     7921 cctaaagata cgcagatgtg tgaggacagc ataagtgacg atgaaccaaa atcagaaaga
     7981 aaataaggag atattcagat gatcaaaaaa tccattgaat taaaggaata taagcgaagc
     8041 gatggagaaa aatattatcc tgtagccagt ttttctaaat cagtatatct ggttggacgt
     8101 gcattgatag ttggtgcaat tggcatatat attttgcatt gggtaacaaa ggttgtacaa
     8161 ctgcttgtag ataccttagt aagtattctt gtctcgttga atgatgttaa cggaacagat
     8221 gatttaggat tgaaagagat gtttaaagag ttactcaatg cgttttttcc aaattattta
     8281 agttattcat ataatgcaca aaactttttt gcaaagctac acttggtaga catctcaatt
     8341 ttagtattag aagcaggagc ggtattcttt gttggatatt tggttacatt gatgttccgg
     8401 catcactatg gagaacaaag accatttttt gatgatatgg aaagtagaag gttgaaaaga
     8461 gaagctctag aagctatgga tgtgaagtat gatccaaaca agattatagg agatgaaacg
     8521 aacaacaaaa aaagaaaacg acacaaaacg caattagaat ccgatgtgct atcaacgctt
     8581 aaaaatatca aaatttctgt tcatacaaga cgtgatctaa gtgtcaatga agatattcga
     8641 gaatatatcg taaaaatccc gcaaccaaaa gggaagaaag ttagagaaac agttttacga
     8701 caaattcaag atttagggtc gatcctgaca agagcaacaa gcggacgagc caaatttggt
     8761 gaaaaagaag atgaagtttc aggcgattat ttcattttcc gagcatcagt aaatattgaa
     8821 agagaacgta aggaaagagt aagtactaaa aacaaagaaa cggaaaaagc aacatcttca
     8881 atcgcagttt caaattcaca attttctttt ccaatcaatc tctttgccga taattccaaa
     8941 aacatccaaa accaaaccga aaaagctaga aaatatgccg aaactcaaaa gcaatcttta
     9001 gaactttatc tatcttctgc tgacgtacct gcaagtctaa cagatatgca cgttgggaat
     9061 acatctattg aatatttgtt tactttatct cgtaccagaa aaattcaact aagctcaatg
     9121 aaagaagaaa ttgaaactta cttgcatata tcaggtgtaa tcgtgcggat caacgcagga
     9181 cgtattcaaa ttactttacc attaggagaa aatgatcgta ttccaattga tgtaccatca
     9241 atgatcaata gcgtgtttta gaagacatca caaatagaaa cagaaaatac aaactaccag
     9301 ttcacaaaca gaaaagtacc gagaaaagcg aggaatagaa atggctatga aattattaga
     9361 gaaagtcaaa aaaagagcag aaaaagctga atccattttg ctagataaag aacctaataa
     9421 aaagcttaat ggaaaattca gtgtcaatat gttgaacaca aaagctaaag acccgacaga
     9481 tgccattctt ggaattaagc ctgatggaac aattgctcat ttttgcatta gcgaaatgcc
     9541 acacttgttg gttgcaggaa cgacaggatc agggaaatcg gtgttactaa atgaaattct
     9601 tgttacagca atgtgtcact caacgcctga tgaattaaaa ataggaattg tagacccaaa
     9661 aagggttgaa tttggaagat acaaaaaatt gccgttcatg ttggctgatc caattacaga
     9721 tatggatgaa gcttatgatt tttttgaata tcttgtgatc ttgatgcatg agcgttacaa
     9781 actgatggaa aaagcagggg tacaaaaaat cacgctatat aacgaatatg ccgaaaaaaa
     9841 tggattagat agatttccct atgtgatctt attagttgat gaatattcac agttggttgg
     9901 aacacataaa gaagtagaag gtttgattgt tcaattagga caaatggcaa gggcagcagg
     9961 tattcatgtt atcctcgcta cacaatctcc tcgatcaaca gttgtcactg gtattatcaa
    10021 agccaatttt ccgtcacgtg tttgccttat ggtagcaagt gatttagaaa gtaggatcgt
    10081 attagatgaa ggcggtggag aaagcctaag tccaaaggga gatatgatta ttaagcacgt
    10141 taatggaagt atggttagag cgcagggagc ttacatcagt aacaaagaaa ttgaaacaat
    10201 cttcaatcac cttagaaata ctatgccaga accagaatat gttgattacc gagcaattgt
    10261 agctgaatct cgtggtgagg gtggagaaga cgaggatttt ggtggaattg gtaaagctcc
    10321 tgtaagaaaa tctgctaaaa gaaatttaag cacaccttta gcaaatccat catctaaaga
    10381 gccatcaatt gaaaagaaga gtgcgtttgc tattaagctt gagaagaaca aagaagaaaa
    10441 agtcgagcag ggagctaaaa cactagaaaa actgcaagca gaaaaactag aaaaagtttt
    10501 aagggcttta ggaaatgctg atcctgaacc gcaaacctca aacaacacga ataataaaca
    10561 gccgattaca tctaaaacta aaccgaaaac tactgatccg aaagtttcag taaacgtgac
    10621 catcacaacc gataattctg agaagaaaca agtaaacgga actacttcac ctatcatgca
    10681 aaaaatgtta aaagaaaaaa aacaggtgca taaatgatca ttgtcagttc ttttttgagg
    10741 tggcttacgc catataaata tacagatgaa gtagttgtga aacgagctaa gaaaaaagat
    10801 aaaaaagcag tgttaactcc taaacagatg cttgatcatg gaattttggg tgtgaaggtt
    10861 ttagaaatga ataaaccgtt tgaatcaaat agtgcaagag aaaagttgct tggtgctgaa
    10921 gcagatgtgg catattttta tcatgacaaa agcaagcatc aaatagctat taaattcaat
    10981 ccaaaatcgg catggcggta tcaagcaaga gacgacttaa aacgtaggaa acttagacca
    11041 atgctctatc cagaaggaaa agaagcattt gatagaaccc acatcgttcc catcgggttt
    11101 catggtacag aatcagataa tcgcctatta gtaggttgga acagcaatca aaatagaatt
    11161 cagatcaata aatttgagaa gaaagtaaga aaattcaacg atctaaaaac aatcctatgg
    11221 tttgcagata tttgtaaaca gccggacgga acagcaaagt gggaagcggt gatatttgac
    11281 gaaaatggta aaaaagtaat gtctgaaaca tggcatgata aagctaaatt tgtttggcag
    11341 tagcagtaga ggtgaaaaag attgctagaa ctttttgaag caaatagaac aggctttaat
    11401 caaaaattag aagtgggaga aactatagaa tataaagata aaaactatat tattatcgga
    11461 atctataaca cgaggattaa cagaataggt gatccacgta taaccagtga cttggtttgt
    11521 caatctgtta actattcacc tgatctgaca agcaggtata agaaatacgt gttattagaa
    11581 tatagacaca agattactga tgaaaatagt gtaaacaaca cacgaaacat atttaagatc
    11641 ggaacagtga tcccttatag taacaacgaa gaaaaaatat tttatcagat ttacggaata
    11701 gaaaagtttg aatatgaaca tgtcgatctg ctagtgacgt accaaatgcg tttaattgaa
    11761 ccttggtcac aggcagaaat tgataaagca gtaaaattga accgattatc aaaatttaac
    11821 gtattaggaa atgcctttta aaaatcgcaa tacacaaaca gaaaaatacc aaaaaattag
    11881 caatgaacgt aggtaagaac atgacaataa ataataataa ccctaaaaaa tataatgctc
    11941 ttgtccaatg ctatatcgaa aacaaaatta caatgcaaga agtcgctgat gaattaggca
    12001 aatcaaagag cctagtatca cacgacttta agcacaatgt ttttccgaac ccgattgagc
    12061 tgaggaaaaa atttcaaaag agagtttatc aagaaatagg taagtggatt gatgaaggaa
    12121 atgcaattga ccctttaatt gaaaaatatc cttttttaaa aagcaaaaat aaaattcaaa
    12181 ggttaattcg tcaaggcgat attcaagcta aagaacctgt tttgacaacc aatgatttgg
    12241 ctaaaataaa tcttcataaa cagattattg aagactttga aaaaaatctt tcaacaactg
    12301 aaattttaaa aaaatacaat attccgagag gaaagctaac atcgactctt tttagagaat
    12361 atggagaatc ggcagttact aaagcaaagg aagtagcgaa tgagagaaaa aaacaaaaat
    12421 agtagtcgat acctagtagc gactgtagtt tttgtttgtg tattgatttt cctgtttgat
    12481 tttttgcaga ttggaaaaag cataattgga aatacattga gtgcaaagga aatggttgct
    12541 ttaggtggtt tgtatgaagg acatttgttc ccgaatatat tcatttctat cgtttctcat
    12601 attaacttgt accatttaat ttctaattta ttgttcttag tagttcttga agaagcactg
    12661 aatgatcaat atgagtggtt aacactattg attgttttaa tcatatcagg acttctagga
    12721 agcttagttc cttttctgtt acaaagcaac atcgtaagta tggggcttag tggaggtatt
    12781 tttggatgga ttgctttatt tgcgattgca tacatccgct ttgatagtaa ggaacattta
    12841 ttactaagtg tttttatatt attgataatc ggcattttac tggtatctac ttttgaatca
    12901 ggcgagacaa acgtcttgtc acatgtaatc ggatttgtct gtgggatgat cttgggattt
    12961 attctaaaga acgtttcatt gatcaagcaa aagaattaaa agggagtgct aaaaatgcca
    13021 aaaaaaaaag aaaagaaaat tccagtgttt ttgtctgtct cagaaatgtt atcacttcaa
    13081 atactgttaa gaaaaaagat tgaaacaata gacgaagaag ttagaaataa aagggctatg
    13141 tacgaacaac gtgaagcgct aagagaacga atccactatt ttgatgatat acacactaaa
    13201 atctatgaag cacgtacaaa taacgaagac gaaagcaaat taggattagc tcaacaacga
    13261 atatttgaat ggctaaatac gaatactgag caagaaccgt taagagcaat tagtaatctt
    13321 tttgaatttg gaacgaagca ttggtttaac gcagtagaaa aggatgctga cttacaagaa
    13381 gcctttgaca cgctaagtaa ggatagcaaa tccatgcgag atttattgat tacttatcta
    13441 aatgagcatg agtttgaaca aacgaaaacc aaaaagaaaa ataaagaata gaaagttgtg
    13501 atgaatcatg ctattaaccc tgttaggtta tttatgttta attcttgctt tttttgggac
    13561 aatcgctata attattggtc aatttcaaac aaataatccg attatgcgta acataggtat
    13621 tggaattgtg attgtaacct ttattgtgtt taatgccact aagatttttg gaagtaacga
    13681 taaacaattt ggtatatcgg gagttaataa tagttatgct ccagattctt tgggaagggt
    13741 agtaatagat ggaacatttg aaaatgtaac gagttataaa aatgtttcgc tgacagtgaa
    13801 ggaagatggc aagaaagcat cagacgacgt tctcgctact attaagccgt cacaatcaca
    13861 atttgaagtg ttttatacag tcaaagagaa tagccctgta cacaagctga cattggtttt
    13921 taacgttgat ggagaagaat actataaaga tgtaaaaata aataaaacat caacgaaaaa
    13981 gattatcgaa tcagcgaaaa aagaagtagc tacagaagaa agtacaactg aatcaagttc
    14041 tactgtcgaa gcggaactac cagataaaat tgattttgat cctgaaaaag tcaaatcgtt
    14101 tgaatctgtt tctggttcta tggctgatcg gtttaacact gaagtcttca aagctatcaa
    14161 aactgttaaa ttggatactg aaaagcaaat gattaatgtt accttattca attcagttga
    14221 atctatggat caggataaaa aagttacggt tgcaagagaa ctaggaaaaa gagtaattgg
    14281 tttgtatcgt ggtactgcta aaccagattc agacggtgca gatttgccag ttcatgtagt
    14341 ttatgaagac ggtaaagttt ttggagattc tacagcaaac gaagcattaa cgattaaatt
    14401 ggctaaatag ccagaaagtt ataataaaca aatggtgtca caccaataat atataggtgt
    14461 gacaccttgg agatgatagc tgatggtagt aaataaagaa aaagttagaa agtatgtgac
    14521 tttaaaaata gcttctgtta aatatttaga aaagcgagct aaagaagaag aaagagcaag
    14581 aggtaagaaa gtaacagttt cagagttgtt agaagaattg atcgagaatg atcaaacaat
    14641 aagacgtgca tttactaata ggtctatcag ataacaatat aattaataaa aaagaaagag
    14701 tgatttggtc ttagactaaa tcactctttc tatataaagt ttctttacac ttattccgaa
    14761 taatgttaaa attctttata cgatatatag ttacttgtca gagaaagaga taaccagcaa
    14821 tattggaggg ataataagtg gaaaacaata atgatattag acattttagc attggggaaa
    14881 gagttacaac agtaagtgaa ttatcaaagg attatagaga taaagaggga acgattattt
    14941 ctcttaataa gaatcgaaga ggcgtttggc aatatgttgt tgatttaggt gatggcgtta
    15001 cagttgagtg taaagatagt caactgatca gaaatgatgg cagtcattcc attgaaaaag
    15061 caattcttgt atacccaaaa gtatcaagta agatcatgaa tgactggttg cttggaaaat
    15121 ttggtgagta caataaaaaa gaaatcgcag aagtgatcgg caaatcttat ccaacagtat
    15181 tagggtatct aaaaaagata actaacaaag aagcgaaagt tgaggatgtt ttaacagtaa
    15241 gagaacttca gtcattaatc gaatatcgga ataaacaaat aacaaatcct gagtacctta
    15301 aaaaagaaga tggtctattg aaacaattgg aacaggtaaa taaaatccgg aagatgcaaa
    15361 aggtagctcc tttaagccta gataacttta aaaaaattta tttgaaagag atcgaaaaag
    15421 actaatgtgg agtgtcactt taccgtttca acctgtatct gcaccaagac caagtgcaag
    15481 aaggaatgtt tctgatgcaa ctaatgacgt agcagagaaa aggatttcaa cctattatga
    15541 taaaaagtat ctcgattatt tgaaaaaaat aaaaagcttt atcgaagatc aaggtttgtt
    15601 agatgatgaa ttttactcga tcgtaaatga tccgatgggt gcaattatgg aagtggactt
    15661 ttactatcag ataccaaaaa gctataaaaa agtctataac attatgaaga cgacagcgcc
    15721 agacttagat aatttagtaa aaagtgctat ggatggtatt ttcaacatct cagctatacg
    15781 agatagccat atcacaggct taattgcttt taaatataat gtggcgaacg aaggaaaaac
    15841 agttgtgagg atcaaacgat attcagaatt tgagtgggat acttctgaag gattgaacgg
    15901 agatatatgg gaagctacat ttgatttcaa accagtagca acgccaagac caaagtctaa
    15961 atttagagga aatgggatta tcacttacta tccaaaagag tacaacgact atttggaaaa
    16021 tatcaagaat acgcttaaag agaaagattt ggtaaatgat caactgaaaa aggtaatgaa
    16081 tgctcctatg ggggttatag ctcagataga ttatttttac caagtgagaa aagataagaa
    16141 aaagcttgat tcactgatgc gtacttcaca gccggatatt gataatttag tcaaaggaac
    16201 attggacgga atttttaaca aggaaatagg catcaaagat agcagggttg tagggttaat
    16261 tgcttttaaa tacaatactt ttgaaaagtc gaaaacaaac gtgagactac aggagatggg
    16321 ctgatgaata ataatgaaaa aattcttcat gtacttgata gctttgaaac aattcaagaa
    16381 gaactaaaga aatatcggga tgttttggaa caaagatatg attttgtcaa tcagcaaaag
    16441 agtaatcaca tggatttcat tctcaatatg aatgacttga agaagaaatt agttgaacga
    16501 aaagaacaag aaaagttgat caaagcttac tttgaactcg gtgaaaaaga agttaaaaag
    16561 gcaatggaac tgaatgaaga tcgcagggtt ttagatcagc tgttagaaca attattggtg
    16621 atgtttcaaa aaggacgaat agatgaggat ttaattgaag aagggttgag aaagtatcct
    16681 tcaaactcag gtatcggaat agtgctgaaa gcgattgatg aagaagaaat tgaagctttc
    16741 ataccgccag aggattttga atcggcaatg gaatatataa aatattatag ccaagggatc
    16801 actgctttta gagagtttga tcctgaagat gttattcatg acttgaacaa cctaaaagaa
    16861 tggtgtgaaa gttatgaagt cgatgattcc ggattggact acttgatcag tattatggaa
    16921 atcgaagaag agatgccgga taagcctgat ccaacagata ttttagaatt gatccatgaa
    16981 gctagaaatc caattgccta tatttctaga ggatatactg tattggaata ctataagcct
    17041 tatatatcag cgatgaacca tttaagacgt gtattaaggg aaaaacgtga ataccgctca
    17101 gtgttgaatg caacgaatag attagaaaaa gctgtttcag agctagaggc ttattatcgt
    17161 gaacattacc tgcaagcagg tggtatgcca agaaatacga aagcaaatat cagtaggtat
    17221 ataaaaaaag ctgagtaggc ggagttagcc cgtaaaatgg caaaaggtaa cctgaaaggg
    17281 gcattttgaa tgaaaaggga tgtttttgaa ttgtacgtat tagaacgtct agaagacggc
    17341 atgatggcac aagagataat tgatgaaatt gatgatatga tcagcagatt tggtcaattg
    17401 gagttgaatg atggaaacaa aaatccggta gtgctaaaac aattttggtt gaacaatttt
    17461 tcgtataagg attttatcaa agaaaaggca aatacattgt actgactaaa ttcatgagga
    17521 gcagtgatga gagcttattg ctaagagcag ttaggctctt tttttgtttg tgtaaaatta
    17581 tttgttttac aatacacatt tatacacaaa aaatgttgac ggatagtaca caaatgtggt
    17641 aggatatatt tgtaagcaag ttagattaac aagttaaagg aaggaagagc agttatgaaa
    17701 aaagaagaac taatagatat atttcaaatt gttgaacgag caaataatat gggaattatg
    17761 ttttttgatc gtatttcttt aaagatggat ttaagcgttg cccaccaaga atttaatcta
    17821 cgattaaaag ccttgttgaa ttctgatgat gttaatttcg ctcatgatgt tgtaggcata
    17881 cagaaccata tagatagaga aaacaaacgt atgggtgatg gattcttacc aagatattca
    17941 agtttataat ttgaagaaag attcaagagt atgtgtcatt attgataggt actcttgaat
    18001 tggaaatgag gaatgaattt aagacaacaa cgattatgat cttaaaacaa tcgtttatgt
    18061 aattgacaat cttacgaata attttcatgc aataatgaca ataaaaatga aaaggggtaa
    18121 aatcaaatga aaaaaatttt agtaggaatt ctattcacaa tggggctagt gctattatca
    18181 ccttcagttt cagcagaaag ctctatttcc atgtatcgta tgtacaatcc caatagcgga
    18241 gagcatctat acactagaag cgttggagaa agagacaatc tgaaacgtta tggatggcat
    18301 tatgaaggtg tcgcatggca agctccaaca agcggtactc ctgtttatcg tctgtataat
    18361 cggtataacg gtgaacattt ttacaccatt gatgccggag aaagagataa gatttcgcac
    18421 acaggatgga cgtacgaagg tgttgcgttc tattcctaca acggagcaaa tagagttcct
    18481 gtgacgcgct tgtacaacaa atacgtgaat tggcaccatt acacgttaga tcaaaatgaa
    18541 aaaaatatca ttagtcgaca agggtggaaa atcgagggaa ctggttggta tgcaatgcca
    18601 aacagcgcac caaatcctgc acctgctcca agcaatgaaa aagtgttaaa tgttcctgtc
    18661 atttggcaag gtaatacgat gttgtgtgaa ggtgcttccc tgttagaggg attgcactac
    18721 aaaggagtag cgacaaatca aaacttaacc agttttgtaa actatatgcc aagagctaat
    18781 gacaataatc catatcatgg ctattctggc gaatggcgct acaacgtaaa tggtacgtat
    18841 caaggcatga tgcctgatcc agtagttaaa tgggcaaaaa atgttggtgg taatgccgtt
    18901 aatatcactg gttcaggaac gaatgggatc aagaatgaaa ttcgcaatgg taatccggtt
    18961 gtaacttgga ttacttataa ctatgcgacg ccagagttca aacaaatgcc ttgggggcgt
    19021 gccgtttgga atgggcatgt tgttttggta gacggattta agaatggcgc ttaccatgta
    19081 gtcgatcctg tatttggagc aaaatggatc aatgcaggca cgtatgaaaa atcgttcaat
    19141 gtaactggca tggcggttgc tgtacgttaa ttgatagaat taataaataa atgaaataag
    19201 agcttaatta ctgaaatggt aaataggctc ttgtttttta ggaaaaaact gctttttgta
    19261 ataagaatat ataacaaaaa aatgaattta gtacacaaaa gtggtatatt aaaaccgtag
    19321 agattatgcg aagaggtaat tttgatgaac caaagaagaa gaaaaagact agaagaaata
    19381 agcgagcgac tatccgagtt aaaggatgag ttagaagtaa ttcaagaaga agaggaagag
    19441 tacaaagaaa atatacctga aaatctacag ggatcagaaa gatatgaaaa agcagaagaa
    19501 tccgttgatc aacttgaaga agctgttgat ggtttatccg acatattaga attgattgaa
    19561 gatatagcta gctagagatg attaatgaag atttcaggag tgagcaaaaa atgattgata
    19621 tttcactgat agaaaagcta cttttttcaa ataagtacac agccaaagag ctggctacaa
    19681 gatggcaaat atccgaatca gctattaaag cctatcgtca aaataaggga agcgtaggat
    19741 ggcgtgattg gaaaggaatc agcattggaa aagcagaaga cataatgaaa cgtgaaggat
    19801 ttcaagctga caatgattct aaaaaagttg aatagtttaa tagtcgccaa gaaaagtacc
    19861 aggacgaggg gaaaggtaga ttacaaagga aaacactttt aaataaactt cgcaaaagca
    19921 taaggttatg agtggctagg gctatacgaa tgtgatgaaa acaaagggct ttaatgtcaa
    19981 agaaaacgca ataggggggg aataaaattt ttaaaaaaga tatttatgtt tggtttaatg
    20041 gaaaaaaata tatgactact aacaaaaggt ggtcagagct agtacaaaat acagttaaaa
    20101 aagtcatgcc agatggatat catttcaact atatacaatg ctcacaaaca actgaaagta
    20161 tttatttttc aatattaaat gagaatagac tctattattt cagggtaagt tcacaccata
    20221 ggaaaaagag agaagataat gttacgacat ttttatcggg aaaatacaat ggcttcaaag
    20281 agttaagtga agcaattggt gagtacctaa aaagtaatag atttgtcaga atttctgaca
    20341 aacatatatt gatcttgtta ctttatttga ataatagctt caaattgttt tggagagacg
    20401 acactttcta ctatattgag aacggcatgg atatttattc tggtatcaac ggcatattaa
    20461 aacattatat taaggcgtta cttcgatatg gggttcttta tcaagaagat ttaagtaacc
    20521 aaattaagat tagtaattct ggaaagtcat tactggcaaa atataaaaaa ttgctttctc
    20581 ctatctacaa aggaatagat atggaagctg taaattctga aactttattt aatttagttg
    20641 ttgatgaaaa gtttgcgaat gaatttttta aaagtaaaaa aattgaaaga ctaaataacc
    20701 tgtttcctga aaatgcaata aaagcttatg tagataataa taaatcacga ttatacaact
    20761 ggtttattcc caaacggttg cgaggaaaaa tcaaaattgg cgatcaagtg aaagtaatta
    20821 atctaaatca tactaagatc gttaccgtaa cggagttgta tatagctgaa aaagaagaga
    20881 ttacaaaaat gaaaccagtt attaaaatac tagataacaa agagtgagtg gaatatatgg
    20941 tagcaaaaaa gaatactcta aaagaagctt ctgaaaaata tcaagatatg aaaagacgag
    21001 gattaatacc aaaggacaaa aaaggtatat ggagagaatt tgttaaggaa gtcagtagtg
    21061 atcgaagtgg cggtgacttt gttggacgat atacagcaaa aggttatagc aggcgcttaa
    21121 ggatcaccgt tgaatatgga acaaatgaat cagctacaga gctgaagcaa aggtacttag
    21181 atacacgaag agaagcttca aaaaaatatg atgatttcaa gagcagaggt ctaattccta
    21241 agaagaagaa ggggatttgg cgtgagtttt tcaaagaaga agatagcaat cggaataagg
    21301 gagacttcat tggacgatac acagcaaaag gctacactaa gaatttaggg atcaaggttg
    21361 aatacggaac tggtgaaagt tcatgggaat tgagaaatcg ctatttaaat acattgttag
    21421 agaaatataa agcagaatta ttggagttgt tcgatgttaa tgattttatc gagatggctg
    21481 aaaagtatgg tcatgaagaa ggttttatga tcaatgacat tatggttatt gataatttta
    21541 cggatttatt agcacttgcg aaaaaagcaa gggatttaag agaaacgcag atgtatctat
    21601 tcagaaataa aaacggaaga aaccggagaa ctgaatcgag tgagattgaa gattactacg
    21661 atgcctttac acacgatctg ggagcctttg tagacgagat aagtggtgta atggacaaat
    21721 atagggtcta gaataaaaaa gccggagatg aacagataaa tgggaaacaa tagcatatac
    21781 acacctgaaa tgcagaataa aattaaaaga cttgtcagat tagggtggat gacaagagga
    21841 aaattagatt cttttagtac gatcaaatat cttttgatgg atatttaccg tcagaacaag
    21901 caaacaggaa tacatttttt gggctatgat aagccggaag atgcaccttt ctttgttcca
    21961 agttcagcct atagagccag tacctccggt agcatgtata acttggattt gttcgaggaa
    22021 ggagcaaaga tgatcgaata tgatttcaac gaagcctatt cgaggatcat gagaaattat
    22081 cctttgccgt caaacactta tttgaaagta aaatataata cagatcaagc tattttgaga
    22141 gcgataaata gaccaaaagt aagaccgtat gaaaatttaa aaacgtatat gttctttttg
    22201 gttaattttg aaggaacagc caaagaagga acatatgcta atccaaaatc acagtcgatg
    22261 tttgcggatt atggcgaaaa tcccaaaaca gctaatgagc ctatttggtt atcggagatt
    22321 gagctaacac ttatccttga ttattatgat attaaagaga tggaagtcct agatagccat
    22381 acatttttga cgaaaaaagg attactcaaa gaatattttg aaagaatcga gccattaaaa
    22441 actgatcctg atacgttgaa gctttataaa aatcttagaa cgaaagtatt tggtgctatc
    22501 ggacaaaaat cattaagaga aggagaaaca agcaccttcg ataaatacag tatgtataat
    22561 cgagcgtatt cctccatggt agcaggcgta tttagagatc gtatggttag atatgaacag
    22621 aaatacgttc atagcgcata tgggctatta aaaattcgta ctgatggatt gtatttcaga
    22681 tccgaagtac cagagtttga agagctgtat aaacttggag tagttaagaa aaaagttcat
    22741 acaatatcca aagaagaagt cgaaaaagct ttagaattta aaaataaagg atttgaaaat
    22801 aagggttcct ttgggaaatc taaagatgaa gttaagggtg ttgtgataga cgatgatttc
    22861 gagtttgatt ttgactaaat actctttgga aaagatggta ggaggtaaat atgaagaaga
    22921 aaattgtgga aggaaatgta ggtgttatta ttctaaaaga ttcaactgac tatgctaaat
    22981 caatggcatt aaaagatgaa aaagttgcac gcatggataa agatttagtt gaatttctga
    23041 tggatgatac agaaagatta aaaggggatc agtttttgag tttaatgaaa gacaaaggct
    23101 taaacacata tggtttattt gattataaga aattagctat agagtgggta aaagaaggag
    23161 aaatattttt tattgaagaa tgggagggta ctgagaattt agttcttcat cagtatcttt
    23221 tgaaagctta atctaagtaa aacataagga ataattttag aattgttgga actatctttt
    23281 gaataattta gtatgaaaag aaattatacc tcaaaacaaa gtatcttttt aggtttaagg
    23341 tactttgttt tgaggtataa ttgggtaaaa atgattttga ggtgatttta atgagtttaa
    23401 gtgtacaata ccacaatact atgaataaca tagcatttaa aaaattttca aacgttgaaa
    23461 tagacgtatt ttttgctata tgttcccaat tagaagaaaa agggagtgac gaagtttctt
    23521 tatccttcag acaattgaaa gaactaagca atctcaatat gatgactgac agttctttag
    23581 taaaagtatt aagatcgctt tataaaaaaa tggcaacatt atatttttat agattgtcag
    23641 aagatggtag tgagacatgt tttgttattt ttaaggaata tagtattgat acttctaaga
    23701 gcaatatagc aattaaaact aatgcagaat atatgtatct tctaaacgat ttaacttcaa
    23761 atttcactat ttttgagatg aaagagttta ctagtttgag aagtaagtac tctaaaagta
    23821 tctattgctt gttgaagcaa agtaatttag gaaaacaagt tatcaatatt gatgagtttc
    23881 gaaaattact tgctgtacca aaaagttata aaatgacaaa tattgataaa aaaatattag
    23941 aaccagtctg cgaagaatta tctatatatt tcgatgaatt gagcatcaca aaagtaaaaa
    24001 cagggaaaag tattgtggct tttgaattta cgtggaataa gtaaaacaat tccgactagt
    24061 ggatgttttc cataaccatc atcattcaaa aataaaggat ttgaaaataa gggttccttt
    24121 gggaaatcta aagatgaagt taagggcgtt gtgataggcg atgatttcaa gtttgatttt
    24181 ggctagattt tttttgcaac gtttaagagt aaacaaggta agaaataaga tcgtgaagat
    24241 tgagagtaag gaaaatagag gacgaatgta tgagaacaca ctatatttat ttagaagata
    24301 aaattataga tgaaattccg gcaaaaagtc gagaacaagc tagaaaatta tttattgaaa
    24361 aatacaaaga gatcaatgga gatagtgaag ttccgaaagt ggtcgtaaca gttagaaaaa
    24421 gacaaataaa aaagagaagt acgtttcatg tcagccctga tatatataat cttatagagc
    24481 aacatagaag tgaagataat atttctatct ctcaatatat tggtacagtt attgatgaat
    24541 tgatttcagg aaagtatgat tatgcttttg attttgagga tgaaaaggat taccgaagtg
    24601 ttgctgtctt tcaatctgat tttgagaagc taaaagacta tgctggcagg agaaatgtta
    24661 attttgcaag actactagaa gagttctact ccgaagagtt gggagtagga aatgaataca
    24721 agaaagaaaa ttatgcgaaa ttatctcata agttatccca agcgcttaaa gaagtgacag
    24781 aagttaaagg ttttagaacc atataaaaca gcatagttct caaatatgat tagaaaccct
    24841 attaaagagc aatattaagc caacttactt aaatcgttta attaagaaca gtaatctctt
    24901 ttagtcagac ttttagtctg acttttttgt atttccaaaa atttattctt tacaaaaacg
    24961 aacatacgtt tgtataattg cttgtgaatg ggggaataaa aatgtttgat tacagtagag
    25021 aaccggtgaa tgactatttt ttaatcgaca tgaagtcttt ctatggtgcg aaacgttcct
    25081 aactgaaaag gttaggcaca caagttcttc ttgtggagaa ctgatgattt gaggagtgaa
    25141 atgatggcgt aacgtcctga agcgctccct ctgatactcc gattggcaat tctcttggtc
    25201 agaataagaa ttgtcaagag ctcggtaaag tcggctgaga gttaccgcag ttgcattgct
    25261 gcaatcgaaa gctgtccagc ggtggatagt gtaacctata ggccgggagt ccaaaaatta
    25321 ctcatggtga gaatgtgcaa ttgcactgac aaacttgcga atgtacgaat ctataggctt
    25381 ggggtgcaga aatgtatctt gtccgcacaa aatctatatt gtgacacagg gtgcgacttg
    25441 cggtttagta agaatcgtag atatgaataa ccgtgcagtg ttataggcac tggaaagtcg
    25501 caggttcaga gtgagcacct aaaagtaaat gtatggataa aatcattgga acgttgaaag
    25561 ctagtgacgt ggaggactaa cctcctttga agcggttgag acgaagaata ataagctcat
    25621 tcacactagt gagagtagtg gcacagtacc tatgaagcag tgataacaag ctgtggaggg
    25681 atagccacga gtcaattagt tttactaaca gttatgatgc aatccataga atcgagtatg
    25741 acaaaataaa caccgacatc cgaagggagg cggtgctatg gaaaacgtat cagcatcaca
    25801 ctcaatccgc catatggaat actatggcat gattgacagc tttgacaatc tatacaaaga
    25861 aagtagcgat aatcacgttt tcaataacct tatggaaatt gtaacttcgt cgaataatat
    25921 cttattagcg tatagaaatg ttaaaggaaa ctctgggagc gtatctccag gcgttgataa
    25981 caagaatatt gatgatttaa aagaaatacc taatgcggat tttatcaaaa tcgtgcaaac
    26041 caaattttca gaatacaaac cacaaccagt taaaagagta gaaatcccaa aaccaaacgg
    26101 gaaaactcgt ccacttggta tcccaactat ctgggacaga atcgtacaac aatgtctatt
    26161 acaagtctta gaacctatca tggaagcaaa attccatgat aagaattatg gtttccgacc
    26221 taatcgatcg gcgcaccatg cgttcgctca agcagtacga ttagcacaag tgtctaaact
    26281 tacttttgta gtagatatag atattgaggg attctttgac aacgtaaacc attcaaagct
    26341 tatcaaacag ctctggacat taggagttca agataagtgg cttttaggtg ttattagggc
    26401 aatgcttaaa gcacctatca tccataagga tggccgaatt gagcacccta agaaaggaac
    26461 tccgcaaggc ggaatacttt ccccactcct cgcaaacgtt gtgctaaatg aactagactg
    26521 gtggatatca tcacagtggg aaacccaccc tacaaggcat aattatgatt ggtatcatgc
    26581 cgagaaaggt tactggaaca aaggaaataa atatcgagcg ttgcgtggtt cgaaactaaa
    26641 agagatttat atcgtaagat atgccgacga ctttaaaatc ttttgcagaa aacgtagcga
    26701 tgcagacaat atatttatag ccgccaaatc gtggctaaag gaacggctga aactcgatat
    26761 tagcatagaa aaatctaaag tggttaatct gaaaaaacag aaatccgagt ttctcggttt
    26821 tactatgaaa ttggttcgca aaagaaaaac ctttgtcata gaaacgcaca tgtgccataa
    26881 agctatgaaa aaagtatctg ataatctagc aaaacaaata aaggtaattc aacacacccc
    26941 caccaaagaa gagttacaaa agcagctagt taaatacaac tcgatggtga tagggattca
    27001 tcaatattac aaaaaggcta cccacatcag tgtggactgt ccgaaaatta atcagcgtat
    27061 aatgattatc attaaaaatc gattggacgt taagaaaaat ggacacccgc ctagtgattt
    27121 cgttaagaaa aactatgcat gttcaaagca gttaagatgg attaatggct atcctatgat
    27181 tcctttagga ttcgtcaaac acagccatac gtcattaccc aaaatgggaa tctgcaagta
    27241 cacgcccgaa ggacgtgtgc taatacacaa gtatcttacc tttccaattg atattatcaa
    27301 ccaacttatg aatatgaata accctgacga aagtattgaa tttagggata atcgtgtctc
    27361 taagtactct tcacaacgag gcagatgccg agtaacaaaa gagttcttgg agctagcaga
    27421 tatttactgt caccgtatca tacctaaaaa tcttggtgga aaagataact tccaaaattt
    27481 agttatcatt catcggaaca ttcatcaact tattcatgag aacgataaag cgagagccac
    27541 actccttgta atggaaatga ggattacagg cactcaactt gagataatca accagttccg
    27601 aaaaaaagct aaaaattgtg aattgtcgct gaatctataa ctgtatgata cctgttagta
    27661 gacattgatg gaacgccgta tgcggcgaaa gtcgcacgta cggtgtggag cgggggaaaa
    27721 tctggagatt gtatcaaagg attacctatc gctatctagc gtggaatgta ttgaacgaaa
    27781 tttagaccca atgactacac cgttaattgt catgagtaga tcggataaca ctggttcagg
    27841 tctgactcta gcttcttcac ctactgcaaa gaaaaaatat catatcacta atgttagtag
    27901 agctaatgat ttaccttatc cattaccaaa agaactttat attgttccac ctcgaatgaa
    27961 cttatatata aaaaaaaata tggaagttaa tcagattttt agaaagtatg tcgcagatga
    28021 agatttattg atttattcga ttgatgaatc aattttaaaa gtgacaaaat ccttaaattt
    28081 atttactagt tcagaaacaa gaagcgagag gagaagagaa ttagctaaaa taatcaaaga
    28141 tgagattgag aaagaaacag gactaatagc aacagtcgga ataggagaca acccattatt
    28201 agctaaactc gcactggaca atgaagcgaa gagtagtaaa gaatttatag ctgagtggac
    28261 ttatgagact gtacctaaaa aaatatggaa aataaacgag atgactgact tttggggcat
    28321 tggatcacgc atgaaaaaaa gattgaatca tatgggaatt atgagtatag aagatttagc
    28381 aaattggaat ccctacacaa ttaaaagtca gttaggtgta atcggacttc agttatattt
    28441 ccatgccaat ggaattgatc gtacggatat tgctattcca gcagaaccaa ccaaagaaaa
    28501 gtcttatggg aattcacaag ttctaccaaa agattatacg aggagagaag aagtagaact
    28561 agtagtcaag gagatggcag agcaagtagc cattcgaatt agacaacata attgcaaaac
    28621 tggttgtgta cgcttagcaa ttggtacgtc aattttggaa gaaaaatcag gtttctcgca
    28681 ccagatgaaa ataccaatta cagataatac gaaagaatta caaaattatt gtcttttttt
    28741 attcaataag ttttatgagg ggcaggaagt aagacatata ggagtaactt atagcagatt
    28801 agtatacaca gattccctac aattagattt atttagtgat ccaagaaaaa agattgctga
    28861 agaaaatttg gataggatta ttgataagat tagacaaaaa tatggattta cagctattat
    28921 acgtgcttca tctatgttag aaggtgcaag aagtattacg agaagcactt taataggagg
    28981 acacgcagga ggattaggag gtttgcataa tgattgaata caacgataga ggacgtttaa
    29041 aatgggtagg attttatttg tctgatcata ctgaaagaat caattcagac aatgtacaac
    29101 gtgccaatca taactcggca aaagaacaaa tgacgactga agagattgcg aaagtactga
    29161 agactgctgt cataaaaaat agacctgtct gcattcaaaa agaggaacga aacgctgaag
    29221 gctactatcc agatgatata gtaggcaaaa ttattggaag tgacgagctt ggtgtatttg
    29281 tggaagaaga aaaagtacac tatgatgaaa ttaggaatgt ttcttttgcg gaattagtaa
    29341 aatggagtca gatatgaaca ttattaagaa atacgaattg ggttatgtta cctatgaaga
    29401 gctaatagaa gaaatatggg gatatggtca acaattaatt aattaatcaa gtggggattg
    29461 attgcttttg tttctatatt gaatccggat caggatatca tagatacaga tattatgtcg
    29521 ttccatatcc atcagaataa gttcttagag attatatttt cagttaatgt ataatatctt
    29581 tttgttatca atgtgatata attacattac tttagtagct tgattagtaa atccagcaat
    29641 ttttctatta ttcctagtat cgcaactact agaagtaatt ttgctgtttg attttctaat
    29701 caagcttttt ttgctttatt agaaaacgat atggattagc agaagagtat ttattacaga
    29761 aaaagaatta gataagatgt taccagaaaa gagttctaat attgaactct ttttttatgc
    29821 ctaaattttt tctatcaatt ttgctattca ataaataatt taattaaaaa aagttgtatt
    29881 agtaggagaa tctcctaaag ctaccgcaag ccgtcctagc catcgc
//
LOCUS       DACENJ010000010        29869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.010, whole genome shotgun sequence.
ACCESSION   DACENJ010000010 DACENJ010000000
VERSION     DACENJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.010"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            141..1010
                     /locus_tag="IW748_000315"
     CDS             141..1010
                     /locus_tag="IW748_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1103249.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1219..2244
                     /locus_tag="IW748_000316"
     CDS             1219..2244
                     /locus_tag="IW748_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1103250.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2283..3233
                     /gene="manA"
                     /locus_tag="IW748_000317"
     CDS             2283..3233
                     /gene="manA"
                     /locus_tag="IW748_000317"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1103251.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3327..5030
                     /locus_tag="IW748_000318"
     CDS             3327..5030
                     /locus_tag="IW748_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1103252.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGEIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5032..7065
                     /locus_tag="IW748_000319"
     CDS             5032..7065
                     /locus_tag="IW748_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1103253.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7076..7516
                     /locus_tag="IW748_000320"
     CDS             7076..7516
                     /locus_tag="IW748_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1103254.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7716..8843)
                     /locus_tag="IW748_000321"
     CDS             complement(7716..8843)
                     /locus_tag="IW748_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103255.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8846..9523)
                     /locus_tag="IW748_000322"
     CDS             complement(8846..9523)
                     /locus_tag="IW748_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103256.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9787..11553
                     /locus_tag="IW748_000323"
     CDS             9787..11553
                     /locus_tag="IW748_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1103257.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11568..12005
                     /locus_tag="IW748_000324"
     CDS             11568..12005
                     /locus_tag="IW748_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1103258.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12018..13169
                     /locus_tag="IW748_000325"
     CDS             12018..13169
                     /locus_tag="IW748_000325"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1103259.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13231..13929)
                     /locus_tag="IW748_000326"
     CDS             complement(13231..13929)
                     /locus_tag="IW748_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1103260.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13922..15274)
                     /locus_tag="IW748_000327"
     CDS             complement(13922..15274)
                     /locus_tag="IW748_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1103261.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15497..16897)
                     /locus_tag="IW748_000328"
     CDS             complement(15497..16897)
                     /locus_tag="IW748_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1103262.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17058..17795
                     /locus_tag="IW748_000329"
     CDS             17058..17795
                     /locus_tag="IW748_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1103263.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17920..18399
                     /locus_tag="IW748_000330"
     CDS             17920..18399
                     /locus_tag="IW748_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1103264.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18396..19163
                     /locus_tag="IW748_000331"
     CDS             18396..19163
                     /locus_tag="IW748_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103265.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19393..19962
                     /gene="lepB"
                     /locus_tag="IW748_000332"
     CDS             19393..19962
                     /gene="lepB"
                     /locus_tag="IW748_000332"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1103266.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20066..20668)
                     /locus_tag="IW748_000333"
     CDS             complement(20066..20668)
                     /locus_tag="IW748_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1103267.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20774..21243
                     /locus_tag="IW748_000334"
                     /pseudo
     CDS             20774..21243
                     /locus_tag="IW748_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21349..21933
                     /locus_tag="IW748_000335"
     CDS             21349..21933
                     /locus_tag="IW748_000335"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1103268.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21936..23009
                     /gene="prfA"
                     /locus_tag="IW748_000336"
     CDS             21936..23009
                     /gene="prfA"
                     /locus_tag="IW748_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1103269.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23002..23841
                     /gene="prmC"
                     /locus_tag="IW748_000337"
     CDS             23002..23841
                     /gene="prmC"
                     /locus_tag="IW748_000337"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1103270.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23834..24013
                     /locus_tag="IW748_000338"
     CDS             23834..24013
                     /locus_tag="IW748_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103271.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24035..25060
                     /locus_tag="IW748_000339"
     CDS             24035..25060
                     /locus_tag="IW748_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1103272.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25235..26479
                     /locus_tag="IW748_000340"
     CDS             25235..26479
                     /locus_tag="IW748_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1103273.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26632..27579
                     /locus_tag="IW748_000341"
     CDS             26632..27579
                     /locus_tag="IW748_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1103274.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27713..28342
                     /gene="upp"
                     /locus_tag="IW748_000342"
     CDS             27713..28342
                     /gene="upp"
                     /locus_tag="IW748_000342"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1103275.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28481..28723)
                     /locus_tag="IW748_000343"
     CDS             complement(28481..28723)
                     /locus_tag="IW748_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103276.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28844..28916
                     /locus_tag="IW748_000344"
     tRNA            28844..28916
                     /locus_tag="IW748_000344"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28877..28879,aa:Thr,seq:cgt)
     gene            complement(29035..29673)
                     /locus_tag="IW748_000345"
     CDS             complement(29035..29673)
                     /locus_tag="IW748_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1103277.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29796..>29869)
                     /locus_tag="IW748_000346"
     CDS             complement(29796..>29869)
                     /locus_tag="IW748_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1103278.1"
                     /translation="KNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        10164 a         5260 c         6071 g         8374 t
ORIGIN      
        1 agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt
       61 ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga
      121 cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa
      181 gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg
      241 gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa
      301 gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa
      361 agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc
      421 gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga
      481 ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg
      541 accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa
      601 aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact
      661 taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag
      721 agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag
      781 gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg
      841 atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct
      901 tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg
      961 tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt
     1021 cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga
     1081 gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg
     1141 tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata
     1201 gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa
     1261 gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt
     1321 acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt
     1381 gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa
     1441 gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag
     1501 acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac
     1561 agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag
     1621 caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta
     1681 gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta
     1741 ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa
     1801 ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct
     1861 gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa
     1921 atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat
     1981 tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta
     2041 aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt
     2101 ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct
     2161 gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa
     2221 gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa
     2281 ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc
     2341 gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga
     2401 tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat
     2461 tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc
     2521 cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg
     2581 atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg
     2641 atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag
     2701 ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag
     2761 gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt
     2821 tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg
     2881 cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg
     2941 cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt
     3001 atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca
     3061 aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg
     3121 acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg
     3181 agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa tagttcccag
     3241 atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat
     3301 caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc
     3361 gcgtgtctat gaaacaactg agaaaagctg gacatcacct aaatgaaatg atcatgccaa
     3421 atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg
     3481 atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct
     3541 tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgaaa
     3601 ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta
     3661 tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat gtgaaattca
     3721 aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt
     3781 gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct
     3841 ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac
     3901 ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa
     3961 tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac
     4021 ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg
     4081 cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg
     4141 tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga
     4201 tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga
     4261 tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta
     4321 catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga
     4381 acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt
     4441 ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat
     4501 tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc
     4561 aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac
     4621 ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa
     4681 taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag
     4741 aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac
     4801 aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta
     4861 tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa
     4921 gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac
     4981 attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta
     5041 tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc
     5101 caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat
     5161 cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt
     5221 gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt
     5281 aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca
     5341 gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat
     5401 gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac
     5461 cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta
     5521 gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat
     5581 tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa
     5641 gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg
     5701 ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga gacatataat
     5761 agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct
     5821 gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac
     5881 ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat
     5941 ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat
     6001 gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta
     6061 caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt
     6121 gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa
     6181 gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc
     6241 aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta
     6301 cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta
     6361 aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga
     6421 tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta
     6481 aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat
     6541 aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat
     6601 caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg
     6661 attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat
     6721 cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg
     6781 ttattccata catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa
     6841 catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt
     6901 atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga
     6961 gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt
     7021 tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat
     7081 acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt
     7141 gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc
     7201 aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca
     7261 tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga
     7321 tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc
     7381 tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat
     7441 cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga
     7501 acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac
     7561 ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat
     7621 gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac
     7681 aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata
     7741 gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc
     7801 atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt
     7861 tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa
     7921 gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga
     7981 gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac
     8041 aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga
     8101 ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt
     8161 atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta
     8221 aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag agagattacc
     8281 tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc
     8341 aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc
     8401 gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac
     8461 tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga
     8521 tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg
     8581 ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat
     8641 tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta
     8701 tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca
     8761 aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg
     8821 cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa
     8881 tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag
     8941 aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt
     9001 tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca
     9061 acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat
     9121 gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca
     9181 atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca
     9241 aataatcaac agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag
     9301 accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag
     9361 ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg
     9421 caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt
     9481 ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat
     9541 aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg
     9601 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
     9661 cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt
     9721 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
     9781 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
     9841 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
     9901 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg
     9961 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
    10021 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
    10081 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
    10141 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
    10201 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
    10261 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
    10321 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
    10381 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
    10441 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
    10501 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
    10561 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
    10621 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
    10681 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
    10741 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
    10801 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
    10861 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
    10921 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
    10981 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
    11041 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
    11101 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
    11161 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
    11221 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
    11281 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
    11341 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
    11401 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
    11461 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
    11521 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
    11581 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
    11641 gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag
    11701 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
    11761 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
    11821 tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag
    11881 tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca
    11941 atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg
    12001 cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat
    12061 ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca
    12121 cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa
    12181 aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag
    12241 taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac
    12301 cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa
    12361 catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag
    12421 aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg
    12481 cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg
    12541 aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa
    12601 aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca
    12661 atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg
    12721 aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa
    12781 gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa
    12841 tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac
    12901 cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac
    12961 ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc
    13021 ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat
    13081 tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg
    13141 tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa
    13201 atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa gatagggcag
    13261 atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag ctgccaggtt
    13321 ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc ggtaaatgac
    13381 accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc ccaaaggtcg
    13441 ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat aagtttcatt
    13501 gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct gacttaacgt
    13561 gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca tgtagtagtg
    13621 acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt cgatatagtt
    13681 gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct caaagtcttg
    13741 accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat gtatactaac
    13801 gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca tcaaaaggtt
    13861 tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat tgagttcata
    13921 attagccacg gttcattcct cctttgataa atccttgagc agctagttct ttacgaagct
    13981 gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat tctttgattt
    14041 tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga atcccggcaa
    14101 cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca gctgggatat
    14161 ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg attccgtctg
    14221 cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga ccaatcatat
    14281 cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat tttttcccat
    14341 tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa ccttgtttga
    14401 ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa gcattgtaga
    14461 cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg aaatctgctg
    14521 acacattgtc catccgtacc atatcagtta atcgataatc caattcagga cgtttgaagt
    14581 cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta taatgcaaga
    14641 tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt tgttcacgat
    14701 cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata gaattgaaga
    14761 ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca ccgtctacaa
    14821 tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg aaaatatttg
    14881 taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt gcttcatcta
    14941 tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag aaagtcgata
    15001 caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg aattgctggc
    15061 gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg gtgacaacga
    15121 cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc agcgctagtt
    15181 tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc gacgtttttc
    15241 caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa acgatttgct
    15301 gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt gcttttctta
    15361 ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac
    15421 aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat tattccctct
    15481 tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca tagcctagat
    15541 ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc cgcccaataa
    15601 acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta atccctaaag
    15661 ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc agcgtttgtt
    15721 cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa agcataaaga
    15781 caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata ttgatgttat
    15841 cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta gttgttcgaa
    15901 tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa aaaatgatca
    15961 gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta cgagtcgtaa
    16021 tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg aataaatggt
    16081 ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca aaagctaaca
    16141 cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa cgtttgtata
    16201 atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa
    16261 acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa tccccagtga
    16321 cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa aaaacggcat
    16381 cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa
    16441 gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg ataccagaca
    16501 aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt
    16561 tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca acttgaaata
    16621 atatttccag cagtataata ccgttatcat tgaacacaga attgatatca aaagtcgata
    16681 gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa gaaacatggc
    16741 tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc gtcatcaata
    16801 tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc
    16861 gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc tgacaatagt
    16921 cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca aacagataac
    16981 aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta gaaaaaagaa
    17041 ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa
    17101 aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa
    17161 agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca ttggaattca
    17221 tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat cctaaaagtg
    17281 agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat gagaaaactg
    17341 accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc aaaatggttt
    17401 taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata
    17461 tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac tttggaagtg
    17521 ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga atggatatcc
    17581 aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac
    17641 gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt ggttgcgtgt
    17701 tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca aaagcttact
    17761 taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga
    17821 aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc gtagtaaagt
    17881 aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt agcacctatt
    17941 ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac aggtattgtg
    18001 aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga aacaacacca
    18061 actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga aaccactacg
    18121 acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata tacaactgac
    18181 agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc aaccaatcca
    18241 agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac gactgtagat
    18301 gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat
    18361 acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta gaccttatca
    18421 acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt aaccgtggat
    18481 atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat ggactttggc
    18541 aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg cttgtttatt
    18601 ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc gatgtcactc
    18661 agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa gaatcgatag
    18721 ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct tatgacgaag
    18781 attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt gagatcaaaa
    18841 caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg tatccttatt
    18901 actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga agctactcgg
    18961 acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg gaacctgtcg
    19021 gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat gagccttatc
    19081 gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt tatagtcgta
    19141 gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa
    19201 acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt ttttatataa
    19261 cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa cattgtagaa
    19321 taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga aaatacaagt
    19381 aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct tgtattagcg
    19441 gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg
    19501 gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa ctatgaacga
    19561 atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt taaacggatc
    19621 atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat taatggcaaa
    19681 aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga taagttacaa
    19741 ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc cacctttacg
    19801 agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga taaccgcttg
    19861 atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca aggaaaagtt
    19921 gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag
    19981 ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat ttcttggagt
    20041 tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag aaaagcgaag
    20101 cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta gcagagacaa
    20161 agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta tggactttcg
    20221 gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg ccaggagcta
    20281 aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa agtacaagtg
    20341 atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca gcgtggatcg
    20401 ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc agcatagttt
    20461 ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa ctaacaagcc
    20521 cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt agataagtag
    20581 ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca taatggttag
    20641 cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac tttgaaattt
    20701 taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca aaaagggaga
    20761 atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt
    20821 ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata
    20881 tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag aatatttagg
    20941 ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta ttgtatggga
    21001 ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga agcagaaggt
    21061 tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg gcactactct
    21121 atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt ggaagttttt
    21181 ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag
    21241 tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta aaaaatgctg
    21301 aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat ggcacaacta
    21361 ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa agtcgcacat
    21421 aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga tacaagagat
    21481 ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa
    21541 gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg tgtgctgatc
    21601 gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat tgtggacgaa
    21661 ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga gttatttgaa
    21721 ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg
    21781 ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc tgtttatgaa
    21841 ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg aaaacattac
    21901 tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta caagcaattg
    21961 aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt tctgatacaa
    22021 agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc gaagtttatc
    22081 gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt ggtgaaaaac
    22141 tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag
    22201 aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat gacgataaaa
    22261 atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta ttcgctggcg
    22321 atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg
    22381 aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc accggagaca
    22441 acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa
    22501 ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg ccagaagctg
    22561 aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat catgcttcag
    22621 gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact cacttaccaa
    22681 cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt gaaaaagcaa
    22741 tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa agcgagtatg
    22801 atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc cggacgtata
    22861 atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa aaattagatc
    22921 agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat gatcaaacat
    22981 caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct gtcacgggct
    23041 tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt atttttggaa
    23101 cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat ttcagaagaa
    23161 gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc acctcagtat
    23221 ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc aacactgatc
    23281 cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg
    23341 ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt gaaagcagca
    23401 agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga agtagcgaaa
    23461 gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac attaggacca
    23521 gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag tcggaatgaa
    23581 tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct ttttgctgag
    23641 aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt actaaaatca
    23701 aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa aaaaatattt
    23761 cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg caatgaacga
    23821 atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt tttttattat
    23881 aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac aagtttatcc
    23941 actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac tcagacaaaa
    24001 taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat atgatgaaac
    24061 agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt ttcctaccga
    24121 aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga aagtgttttc
    24181 tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt ttgaacaagt
    24241 aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga acttctggcc
    24301 tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag ttgtgacggg
    24361 aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag aagtaatcga
    24421 actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac caagtccaac
    24481 gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta ttgatgatgg
    24541 agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa cagccatgcc
    24601 tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc
    24661 tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa
    24721 atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg attggtcaac
    24781 tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat
    24841 tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca actcagtaga
    24901 agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa cacttggatt
    24961 agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt acatgaatcg
    25021 cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt attgtcgcat
    25081 tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga aaaactaagc
    25141 gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga tgggaaaaat
    25201 aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa cgtttgaccc
    25261 tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt tggagctgat
    25321 tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta tcttgacaaa
    25381 taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat ttgtcgatat
    25441 cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat ttgcgaacgt
    25501 ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg tagaaccagg
    25561 ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg gatcaccagt
    25621 caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga caacagaagt
    25681 tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat tgatcgtagc
    25741 aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa tcgcagatga
    25801 agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag cagcaggact
    25861 tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata aaacattgcg
    25921 tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa aaataaatag
    25981 tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg gtaaagcagt
    26041 agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa tcatcgccaa
    26101 tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca tttcaggggc
    26161 gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg gaaaagaagc
    26221 agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc cttttgaaac
    26281 actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca ctactcgcgg
    26341 gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat tacaagcaaa
    26401 agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa ctgaaaaatt
    26461 tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt tcaatgttgg
    26521 gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga tgctctctag
    26581 tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt catgactgat
    26641 cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc tcatccgcgt
    26701 atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca agaatcacct
    26761 gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga atggggaaaa
    26821 ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt tattttttat
    26881 attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt gatcagagaa
    26941 cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc accagaagca
    27001 aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa taaattagcc
    27061 aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt aggtggtaac
    27121 atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat atccaagcaa
    27181 ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca tgaattcgga
    27241 gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata tacgacgaat
    27301 gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa tgaactaaat
    27361 gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga tgaaggtgag
    27421 gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg cttccaagga
    27481 atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac gcgtgcagca
    27541 atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt ccgcaggaaa
    27601 acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag tcaatcactt
    27661 gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa aaatgggcaa
    27721 atttcaagtg atcgatcatc cattgattca acacaaacta actattattc gggacaaaaa
    27781 ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga tggcatatga
    27841 agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca ctgaaacaac
    27901 acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg caggtattgg
    27961 aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata tcggtcttta
    28021 tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag aagacattga
    28081 tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag caatcatggc
    28141 aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt tggtagcagc
    28201 acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct atacagcttc
    28261 tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg atgcgggtga
    28321 ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac ttttgtcaca
    28381 tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc tttcgtttgc
    28441 atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt tttagttctt
    28501 tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt tctgcagttt
    28561 catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca gtttgcgact
    28621 tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca atttccgatg
    28681 gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct gcctttcaca
    28741 tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc catcggactc
    28801 tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct tagctcagtt
    28861 ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag ctggcattaa
    28921 aagagaagac agatatatca aggattttta atccattgat acatctgtct ttttgtttaa
    28981 aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa aaatttagga
    29041 gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg cccaacttgt
    29101 aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca ccccttgtat
    29161 caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac tgtaatggat
    29221 aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact tactagaaaa
    29281 aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag gcaaaataat
    29341 aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc atttcaaaat
    29401 ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa aatatttggc
    29461 tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt ttttaccaaa
    29521 aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa taatggatgg
    29581 cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata tccctttttt
    29641 aagttctatt ttttttccta aaatgttatt catgataata actcctacta ttcagttttt
    29701 gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta tggatatttg
    29761 tattgctaaa aatttatctt atatattcat tttgattata ctacttttat ttgtaaaaat
    29821 tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aatttttta
//
LOCUS       DACENJ010000011        29776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.011, whole genome shotgun sequence.
ACCESSION   DACENJ010000011 DACENJ010000000
VERSION     DACENJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.011"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..291
                     /locus_tag="IW748_000347"
     CDS             <1..291
                     /locus_tag="IW748_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ1103279.1"
                     /translation="EILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTI
                     RSSNGMISSLNEARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            358..852
                     /locus_tag="IW748_000348"
     CDS             358..852
                     /locus_tag="IW748_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1103280.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            908..1546
                     /locus_tag="IW748_000349"
     CDS             908..1546
                     /locus_tag="IW748_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103281.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1618..2013
                     /locus_tag="IW748_000350"
     CDS             1618..2013
                     /locus_tag="IW748_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1103282.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            1997..4450
                     /locus_tag="IW748_000351"
     CDS             1997..4450
                     /locus_tag="IW748_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1103283.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4447..6474
                     /locus_tag="IW748_000352"
     CDS             4447..6474
                     /locus_tag="IW748_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103284.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6471..7493
                     /locus_tag="IW748_000353"
     CDS             6471..7493
                     /locus_tag="IW748_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1103285.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7510..8439
                     /locus_tag="IW748_000354"
     CDS             7510..8439
                     /locus_tag="IW748_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1103286.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            8684..8800
                     /locus_tag="IW748_000355"
     CDS             8684..8800
                     /locus_tag="IW748_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1103287.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            8816..10735
                     /gene="tet(M)"
                     /locus_tag="IW748_000356"
     CDS             8816..10735
                     /gene="tet(M)"
                     /locus_tag="IW748_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1103288.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            10833..11021
                     /locus_tag="IW748_000357"
     CDS             10833..11021
                     /locus_tag="IW748_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1103289.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(11079..11432)
                     /locus_tag="IW748_000358"
     CDS             complement(11079..11432)
                     /locus_tag="IW748_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1103290.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            11963..12385
                     /locus_tag="IW748_000359"
     CDS             11963..12385
                     /locus_tag="IW748_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1103291.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            12382..12612
                     /locus_tag="IW748_000360"
     CDS             12382..12612
                     /locus_tag="IW748_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1103292.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            13110..13310
                     /locus_tag="IW748_000361"
     CDS             13110..13310
                     /locus_tag="IW748_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1103293.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            13337..14530
                     /locus_tag="IW748_000362"
     CDS             13337..14530
                     /locus_tag="IW748_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1103294.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(14598..16163)
                     /gene="guaA"
                     /locus_tag="IW748_000363"
     CDS             complement(14598..16163)
                     /gene="guaA"
                     /locus_tag="IW748_000363"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1103295.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(16222..17145)
                     /locus_tag="IW748_000364"
     CDS             complement(16222..17145)
                     /locus_tag="IW748_000364"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1103296.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            17341..17946
                     /locus_tag="IW748_000365"
     CDS             17341..17946
                     /locus_tag="IW748_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1103297.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            18254..19270
                     /locus_tag="IW748_000366"
     CDS             18254..19270
                     /locus_tag="IW748_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1103298.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            19411..20712
                     /locus_tag="IW748_000367"
     CDS             19411..20712
                     /locus_tag="IW748_000367"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1103299.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20758..21420
                     /gene="deoC"
                     /locus_tag="IW748_000368"
     CDS             20758..21420
                     /gene="deoC"
                     /locus_tag="IW748_000368"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1103300.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            21441..21839
                     /locus_tag="IW748_000369"
     CDS             21441..21839
                     /locus_tag="IW748_000369"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1103301.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21905..22987
                     /locus_tag="IW748_000370"
     CDS             21905..22987
                     /locus_tag="IW748_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1103302.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            23199..24761
                     /locus_tag="IW748_000371"
     CDS             23199..24761
                     /locus_tag="IW748_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103303.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24754..25887
                     /locus_tag="IW748_000372"
     CDS             24754..25887
                     /locus_tag="IW748_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103304.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25887..26843
                     /locus_tag="IW748_000373"
     CDS             25887..26843
                     /locus_tag="IW748_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103305.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26942..28108
                     /gene="deoB"
                     /locus_tag="IW748_000374"
     CDS             26942..28108
                     /gene="deoB"
                     /locus_tag="IW748_000374"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1103306.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            28132..28950
                     /locus_tag="IW748_000375"
     CDS             28132..28950
                     /locus_tag="IW748_000375"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1103307.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28967..29674
                     /gene="deoD"
                     /locus_tag="IW748_000376"
     CDS             28967..29674
                     /gene="deoD"
                     /locus_tag="IW748_000376"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1103308.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         9742 a         5218 c         6249 g         8567 t
ORIGIN      
        1 gaaatactaa aaaaatatga aattacagtt cctagagacg aaaatctatc taaattatat
       61 agtaggatac aacaattaat tgaaaaagaa attcaaccta ctgaacttgc tgatatagtg
      121 aagacaacga taaggtcatc aaatggaatg attagctctc ttaatgaagc aagaaatagg
      181 cattcacttg cacaccctaa tacaaatatt attggaaaga gagaagcaaa actaataata
      241 ggtatttcaa gtacagttac agattatatt agcggatacc ttgataagta gcgtcattaa
      301 ctgattgcta ctttttttat gactattttt ataaaaagga tgtgataaga attggaaatg
      361 aaagtctatg tggcaaattt aggacggtat aatgaaggtg aattagtcgg agcatggttt
      421 actccaccaa ttgatgagga agaaatggca gaaagaatcg gtttaaatga ggactacgaa
      481 gaatatgcaa tacatgattt tgagcttcct tttgatgtgg acgaatacac gccgattagt
      541 gaaatcaacc gtctatgtga agccattcaa gaaatcgaag gtacacctat ttataatgaa
      601 ctcaaagaaa ttcaaggcat gtggtttagt agcttggaag aattactgga aaacaaagag
      661 gatattcatt gttatagtga ttgtgattcg atggaagatg tcgcccgtta ttacgtggaa
      721 gaaacaggac agcttggtga agtcccttcc aatttacaaa attatattga ctaccaatct
      781 ttgggacgtg atatggaaat agagggtaat tacctagtta cctctcatgg ggtatttgaa
      841 tattgtcagt aagtttaaca atccgtgtta taattaaatt aataaatagc acggagaggt
      901 ataaattatg gtaaatgtat ttgaaaatct taaactatta aaactagaca tgaaagataa
      961 aggctgggtg atagattcct tttactttcg ttacaaacag caaaattata ttgtacttgt
     1021 taagctattt gaaaaagagg aaaaagttcc agaatatgct ctgttaaaac tagaattttt
     1081 aaaggaaaac gatttttcag atatgctagc tgtctatgct aattcggtaa aattatatac
     1141 agacacgaaa acaataagag aatattttgg tatcgaatac agtagtaact tgggagatgt
     1201 tttatttcaa tttagccaaa ctcttgctcg atttattccg acagaagtta gtgagaagaa
     1261 aaatgaagac caaaaagaag ccatgtgttt ctcattaagt caaagtgatt cagaagatcc
     1321 cagaaaaaaa tattgttttt cagttagaag aaatccttta aagggaaatg gtgagctggg
     1381 aaaacgaagt ccttttaatg acaataaaac gagattgtat agacccagct tatatgaacg
     1441 attagggtca gataccaatt taagtttcag atattcaatg aatccagatg atgaagaaac
     1501 tgatgaaggt attattgcaa aatggacaaa aaataaaatt gaatagttaa agcattgaaa
     1561 gagcaatcac ctattttgat tgttcttttt ctatttttac ggaaaggacg tgaagacatg
     1621 aaaaaaataa gaagttacac cagtatttgg tcagtagaaa aagtgattta tgcgattaat
     1681 gatttacaac tccctttccc aattaccttt acgcaaatga catggtttat cgtttctttg
     1741 tttgtcgtca tactctttgg cagtttacca ccactttctc tgattgacgg agcactactc
     1801 aaatacttag ggattcccat tgttctcact gggttcatgt gccaaaagac ctttgacgga
     1861 aagaaaccct atggatttct aaagtctatc attacctatg ctctacgccc taaagtaacc
     1921 tacgctggca aacaaatacg attaaaaaaa gaaacaatga atgaatcgat tacgattgta
     1981 aggagtgaac aactagatgg catatccgat taaatatatg gaagataatc tcgtatttaa
     2041 tcatgatggc gaagtattcg cttattatga attaattccc tataattaca gttttttaag
     2101 ttccgatgaa aaaattcaag ttcatgataa tttctgtcaa ttgattgccc aaaatcgtga
     2161 tggcaagatt catgctctgc aactagcaac cgaatcaagt attcgagcca cacaggaaca
     2221 atcgaaaaaa gaaatcactg gacgattaaa agagatagct tacgaaaaaa tagatgaaca
     2281 gaccgaagcg ttaatttcta tgattggtga caatcaagta gactatcgct tttttattgg
     2341 ttttaaactc tacctcaatg aacaggaagt atccgtaaaa aatgttggga aagaaattca
     2401 aaaagcattt gcggaattct ttcatggtgt gaatcacaaa ttaatgggcg attttgtttc
     2461 tatgcctatg gcggaaatcg aacgcttttc taagatggaa cagttgttag aaagtaaatt
     2521 atccagacgc tttcagattc gtaaattgga gaaagatgat tttggttata ttattgaaca
     2581 tctttacggg caaacaggtg tcccttttga tgactacgac taccatttac cgaaacgata
     2641 cgaagataaa aaagtgttgg taaagaaata cgatattctc aaacccactc gctgtttaat
     2701 cgaagaaaat caacgctatt tacggattga acaggaagat accactactt atgtggcgta
     2761 ttttacaatt aatagtattg ttggtgaatt ggatttccct aatagtgaaa ttttttatta
     2821 ccagcaacag cagtttactt tccccgttga taccagtatg aatgttgaaa ttgtcgccaa
     2881 taaaaaagcc ttatctaccg ttcgcaataa aaagaaagaa ctgaaagatt tagatagcca
     2941 cgcatgggaa agcgacaacg aaaccagtac taatgtggtg gacgctttag aatctgtcga
     3001 tgaattagaa agtaatttag accaaaccaa agaatctatg tacaaactaa gctatgtggt
     3061 acgggtatca gcaaagaatt tggacgagct gaaacaacgt tgtgatgaag taaaagattt
     3121 ttatgatgat ctgtctatca aattagtacg accttttggc gatatgctag gcttgcataa
     3181 tgaatttatt ccctcaagca agcgatatat caatgactat attcaatatg tcacgtcgga
     3241 ttttttggct ggtcttggtt tcggtgctac acaaatgatt ggtgaaaatt ctggtattta
     3301 cgtcggttac aatctcgata caggcagaaa tatttatctc caacctagcc ttgcaagtca
     3361 aggaattaaa ggctctgtaa caaatgcatt agcgtccgct tttattggtt cattaggtgg
     3421 cggaaaatca ttctctaaca atatgctggt ctattattca gtactctttg gcggacaggc
     3481 aaccattgtt gacccaaaag ctgaacgtgg tgaatggaaa gaaaccttac ctgatattgc
     3541 ccatgaaatc aatattgtta atctcacgag tgagaacgac aataaagggt tacttgaccc
     3601 ttatgtgatt ttaaaactcc cgaaagattc agaaagcctt gccattgata ttctcacgtt
     3661 cttaacaggt atttccagcc gagatagcga gaagtttcct gtcctgcgta aagctattcg
     3721 gagtgtaact caaagcaaac aacgagggtt gttattggtg attgatgaat tgcgaaaaga
     3781 agatacgccc atcagcaata gtattgctga ccatatcgaa agctttgtcg attatgattt
     3841 tgcccatctg ttattcagtg atggcagtgt cacccaatcc attagcttgg aaaaacaact
     3901 caatattatt caagtggcag atttggtctt acccgattcc aatagctctt ttgaagaata
     3961 cactaccatg gaattattaa gtgtggcaat gctgattgtc attagtacct ttgctttaga
     4021 ctttatccac tctgaccgtt ccatttttaa aattgttgac cttgatgagg cttggagttt
     4081 tttacaagtg gcacaaggaa aagctctatc tatgcgattg gttcgagcag gacgtgccat
     4141 gaatgcaggg gtctatttcg ttactcaaaa cgcggacgat ttactagatg aaaagatgaa
     4201 gaataatatt ggcttgaaat ttgcctttcg ttccactgac ctagtggaaa tcaaaaagac
     4261 cttagaattt tttggtgtgg acaaggaaga tgaaaacaat cagaaacgct tacgtgaatt
     4321 agaaaatgga caatgtttag tttcagacat ctatggtcgt gtaggggtta tgcagtttca
     4381 cccgattttt gaagagttgc ttcatgcctt tgataccaga ccgcctgtgc gaaatgaggt
     4441 ggaagaatga cacaaaaaca aaagaaattt cttcactata gttggataac gctcctttgt
     4501 gtgggcgtta ttttactttt attaggcacg ctgggcaatg cagtacaagc gacaggctta
     4561 gttgatgaaa ccattgatac aagcaatgaa tactccaagt atggtttgaa tcaatatcag
     4621 cttgattatt atgtggacaa tagctggggc tggcttccat ggaattggag tgatggcata
     4681 ggacaatcgg tgatgtatgg attgtatgcc attactaatt ttatttggac aatttctctt
     4741 tatctatcga acgccacagg ttatttggtg aaagaagcct atgccttaga tttcattagt
     4801 caaacatccc aagcaattgg cgaaaacatg cagacgattg caggcattac accgaatggc
     4861 ttttccagtt cagggtttta tgtcggtttc ctccttcttt ttattctcat tgttggaatc
     4921 tatgttgcct atacaggatt ggttaagcga gaaacgacga aagtgatacg agctgtttta
     4981 aactttgtcg tggtgtttat tctatcggct tcctttattg cgtatgcacc cgattatatt
     5041 gcgaaaatca atgatttttc caaagatgtc agtaccgcca gtctgaatat tgggacaaaa
     5101 attgttcttc ctaacacgga tagtcaaggg aaagacagtg tggacatgat acgtgacagc
     5161 ttattctctg ttcaagtaaa gcaaccatgg ctattgcttc aatatggctc cactgatgta
     5221 gaagcgttag gggaagaacg tgtggaaagt ttattgtcta ccagtccaga tacaaacaat
     5281 ggtgaagata gagaaaatgt agtgattgaa gaaattgaag atcatgacaa tacctattta
     5341 accctaccga agacgatttc cagattagga acagtcttct ttttgtttgt attcaatatt
     5401 ggtatctcga tttttgtttt ccttctaaca ggaatcatga ttttttcgca agtgctattt
     5461 attattttcg ctatgttttt gccaatcagt ttcctattaa gtatgttgcc gagttttgat
     5521 ggcatgggca ggcatgccat taccaagtta tttaatgtga ttatgacaag ggctggtatt
     5581 accttgatta ttaccgttgc ctttagtatt tccactatgc tttattcgct gtcagcaagt
     5641 tctccctttt tcatgattat gtttttacaa attgtgacct ttgcagggat ttattttaaa
     5701 ctgggcgatt taatgagcct attcacttta caaagcaacg actctcaaaa catgggcaga
     5761 caagtgtttc gcagacctcg tcaaatgatg aatcgacaaa ccagaagatt tcaacgcagt
     5821 atgagtaaga tttttcgtgg aaaagcaggg tcaacgaagc aaaagacaat gacaacaaag
     5881 cagaatcaaa ccaataaagc taaccatact cgtcaaaatg aacggactac accaaagaaa
     5941 gaaacagcac tttccaatgt gaaaaagcaa caaggcggta tcgggcaacg attaggtgaa
     6001 aagacagccc atgtattgga cacgaaaaat cgtctagtca ataaaagaaa gcaggcaaaa
     6061 caacagatac aggcgacacc aacccatgtc aaatatgccc ttcatcaagg aaaagagaat
     6121 gtcactcata atgtaacaga ctttaaacaa tctatggcag ataccaaagc tcaacgcaaa
     6181 caacagcgta tgaaccagca ggagcaaaaa cgaaagaaca ttgctcaaaa gcgtttggag
     6241 ctggataaag caaaagaaaa aagacgaaaa gaaaacccat catctcctgt tcagaataac
     6301 cagcagcatg ttcgcaagcg tcagcaaacc gtcacgcctg ttttgtcaca aaacactaaa
     6361 cgtgaaaatc atcagcgacc attgaaaaaa gagcaaaaac aaaagacacc accgaagcaa
     6421 ttcaatcaga ggaagctaaa taaaagaacg gataagcgag gacaacaaac atgaagctaa
     6481 aaataacagt gatttgtagt gtggtcttct ctctcttctt ttttctctta ttattcctag
     6541 tcattttctt tgcagatgat acggacagtg gcgaaaacaa caaggattct tctattccac
     6601 aaggcggtgt gaccgtttca ccagaagtgt tggcacaccg tccgttgatt gaaaaatatg
     6661 gtaaggaata tgggattgaa gactatgttt cttatatctt agccattatg caagtggaat
     6721 cgggcggtac agcagaagat gttatgcaaa gttccgaatc tttgggttta ccgcccaaca
     6781 gtttgagtac cgaagagtcc atcaaacaag gggttaaata tttcagtgaa ttattaacca
     6841 gtgcagaaca acaaggagtg gatatagatt ctgttatcca atcttataat tatggcggtg
     6901 gctttttaaa ttatgtgagg agtcatggga aaaaatatac ctatgaatta gccgaacagt
     6961 tttctaaaga gaagtcgggt ggtcaaaaag cagactatcc caatcctata gccatacctg
     7021 tgaatggtgg ttggcgatat aactatggaa atcagtttta tgttcagttg gtttcacaat
     7081 acctaaccga cacttcccca acagaatttg atggtgaaac cgtgcaagtc attatggacg
     7141 aagccttgaa atacgaaggg tttccctatg tctttggagg ggcgtcgcct accacctctt
     7201 ttgattgtag tggcttaata caatgggtgt atgacaaagc tggcatttct ctccctcgtg
     7261 tcgcccaaga tcagtatgac gctacgcaag aaatctctat ggaagaagcc caagcaggcg
     7321 accttatctt ctttcattca acctataatg caggcacata tgtgactcat gtggcgatat
     7381 atttagaggg aaatcgcttt tatcatgcag gcgacccaat cggttatggc gacttaagca
     7441 gtcgttattg gcaagaccat ctgattgggg ctagacgtgt tattcataac taagaaagga
     7501 atgaatcaaa tgattataaa aatcgagcga aaagaaaaga aggagaaacc aaagaaatct
     7561 aaaaaaccga gcaagcaaag aaaagttccg atgattaaag ttggcacgca taagaaatta
     7621 acctttgttt tatgggtttt attgattggt agtgtcggat ttggaatcta taaaaatttt
     7681 actgccattg acactcatac tgtccgtgaa acagaaatca ttaaacagca gattgtcgat
     7741 accaaccaag tagaaagttt tgtgaaatcc tttgcgaaag actacttttc gtggcaacaa
     7801 tcccaagaag ccattgataa acgaaatgaa aaattgaccc attatttgac ggaagaattg
     7861 caggtattaa atgaagaaat gattcgtaag gatattccta ctagttctag tgtgaacgat
     7921 atacaggtgt ggcaggtttc tcaagtgaat gagaacacct ttgaggtact gttttctgtt
     7981 gaacaagtta ttaccgaaga caaagacaag gaaactatat cgtctagctt tcatgtggtt
     8041 gtccatatag atgaatcaga taatatggtt attatcaaaa atccgacaat gagtaagaaa
     8101 cctcaaaaat cggactacca acccaaacag ctagaaagtg accatacagt agatacagaa
     8161 acgatggacg aaatcactag ctttttagaa acgttcttcc agctctatcc taccgccaca
     8221 gaaaaagaat tgacttacta tgtaagtaat catgtgttac ctatgattaa caaggaatat
     8281 gttttcgagg aattggtgaa tcctatcttt accagaaaag ataaccaagt aatcgtgaat
     8341 gtcgctgtga aatatttaga tcaagaaaca aaggcaaccc aaatctcgca atttgagttg
     8401 atattagaaa aacaagataa ttggaagatt gtaaaataac aaatattggt acatgattac
     8461 agatactttg taatcatgta ctctttttga taaaaaattg gagattcctt tacaaatatg
     8521 ctcttacgtg ctattattta agtgactatt taaaaggagt taataaatat gcggcaaggt
     8581 attcttaaat aaactgtcaa tttgatagcg ggaacaaata attagatgtc cttttttagg
     8641 agggcttagt tttttgtacc cagtttaaga atacctttat catgtgattc taaagtatcc
     8701 agagaatatc tgtatgcttt gtatacctat ggttatgcat aaaaatccca gtgataaaag
     8761 tatttatcac tgggattttt atgccctttt gggtttttga atggaggaaa atcacatgaa
     8821 aattattaat attggagttt tagctcatgt tgatgcagga aaaactacct taacagaaag
     8881 cttattatat aacagtggag cgattacaga attaggaagc gtggacaaag gtacaacgag
     8941 gacggataat acgcttttag aacgtcagag aggaattaca attcagacag gaataacctc
     9001 ttttcagtgg gaaaatacga aggtgaacat catagacacg ccaggacata tggatttctt
     9061 agcagaagta tatcgttcat tatcagtttt agatggggca attctactga tttctgcaaa
     9121 agatggcgta caagcacaaa ctcgtatatt atttcatgca cttaggaaaa tggggattcc
     9181 cacaatcttt tttatcaata agattgacca aaatggaatt gatttatcaa cggtttatca
     9241 ggatattaaa gagaaacttt ctgccgaaat tgtaatcaaa cagaaggtag aactgtatcc
     9301 taatatgtgt gtgacgaact ttaccgaatc tgaacaatgg gatacggtaa tagagggaaa
     9361 cgatgacctt ttagagaaat atatgtccgg taaatcatta gaagcattgg aactcgaaca
     9421 agaggaaagc ataagatttc agaattgttc tctgttccct ctttatcatg gaagtgcaaa
     9481 aagtaatata gggattgata accttataga agttattact aataaatttt attcatcaac
     9541 acatcgaggt ccgtctgaac tttgcggaaa tgttttcaaa attgaatata caaaaaaaag
     9601 acaacgtctt gcatatatac gcctttatag tggagtacta catttacgag attcggttag
     9661 agtatcagaa aaagaaaaaa taaaagttac agaaatgtat acttcaataa atggtgaatt
     9721 atgtaagatt gatagagctt attctggaga aattgttatt ttgcaaaatg agtttttgaa
     9781 gttaaatagt gttcttggag atacaaaact attgccacag agaaaaaaga ttgaaaatcc
     9841 gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct gaacagagag aaatgttgct
     9901 tgatgccctt ttggaaatct cagatagtga tccgcttcta cgatattacg tggattctac
     9961 gacacatgaa attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact
    10021 gttgcaagaa aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga
    10081 gagaccgtta aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg
    10141 ggcttccatt ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag
    10201 ctcggtttct cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg
    10261 ctatggttgc gaacaaggat tatatggttg gaatgtgacg gattgtaaaa tctgttttaa
    10321 gtacggttta tactatagcc ctgttagtac tccagcagat tttcggatgc ttactcctat
    10381 tgtactggag caagccttta gaaaagctgg aacagaattg ttagagccat atcttagttt
    10441 taaagtttat gcaccacagg aatatctttc acgggcatat aacgatgctc ccaaatattg
    10501 tgcaaatatc gtaaatactc aactgaaaaa taatgaggtc attattattg gagaaattcc
    10561 tgctcgatgt attcaagatt atcgcaatga tttaactttt tttacaaatg ggcttagtgt
    10621 ttgtttagca gagctaaaag gatatcaggt taccactggc gaacctgttt gccagacccg
    10681 tcgtctaaat agtcggatag ataaagtaag atatatgttc aataaaataa cttagtgcgt
    10741 tttatgttgt tatataaata tggtttctta ttaaataaga tgaaatattc tttaatatag
    10801 atttgaatta aagtggaaag gaggagattg ttattataaa ctacaagtgg atattgtgtc
    10861 ctatttgtgg aaataaaaca agactacgaa tacgagtgga tactatactt aaaaatttcc
    10921 ctttatactg ccccaaatgt aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa
    10981 taacaatcaa agagccagac gccaagacgc agagccgata atttgagaaa tgaaactctc
    11041 atcttatcgg ctctttttgt ttatctgaat tttactgact agccttcaat atttcttgct
    11101 tcattgatac cttttgctag tgcttccatt aaggatagtt ctttgtctgt aaagctatcc
    11161 atgtattttt ctacctgtaa ccttcgagtg cttttaatca aattatctgt aggtaagaaa
    11221 aattcatcaa tagatacatg gagtaatgat acaagatcat acagcacttg tgtacttggg
    11281 tgttgtcctt tgttttctat atttgttaaa taacgtgggt caatttcaat cattgttccc
    11341 acttgttcac gagtgagacc ccgtttcatt cgagcctctt taatcgctaa accaacggct
    11401 ctaaaatcgt atttatcttc ttttttacgc atactaaatc acctctatac attttactgt
    11461 tcctatataa ccataaacag gtatagaaaa acatgttata tagtttatga gttcctatag
    11521 aacactgtgg aatttattaa tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt
    11581 atttacctat acaactttat aaaatttagt catactccta gaaagtagat aaaattggcg
    11641 acttaatgga gggaaaagtc gccatgagaa agataatctg tcatctagtt ctcttaatta
    11701 agttcttatc aaaaaaactt atccacctgt actggataga tatgaccatc aatgtggttt
    11761 tgcagattac atagatagaa aaatcaaagc atatgaacag agaaaacaat ctgtttatgt
    11821 gcttttttgg tcacttaata aatatctttt aggatttctc ttcatttatg caacaaatcc
    11881 ccctttcgtg tcggggttta gtatgaaggg agataaagaa gcacatgcca ctccctttca
    11941 cgacagaaag ggggtgaaaa gcatgaaacc atcttctttt caagaaacca tagaaaatca
    12001 gtttgactat atctgcaaac aagttattga agatgaacga aaagattact tcaaacatct
    12061 agacagacta tcagcaaaag aagtctcgtt ttctgatata gataactacc ttgtcaactc
    12121 tttttctact gttgatacgt acgtcactga tttccaacta tttaaattat ttggattaag
    12181 aatcaatgtt gaaagtgatt tattaagtga agccctgcgt catctttcag agaaaaaacg
    12241 gaacattatt ttacttcatt actttatgga tatgagtgat gtggagattg cggagttact
    12301 aaatttgaat cgttcgactg tttatcgtca tcgaataagt ggactagcca tgattaaaga
    12361 attcatgaag gagtgtaaag aatgaaaaca gcttatccaa gggttccctt ccccctcatt
    12421 gtaaaggcga cagatggcga cgtggaagca attaatcaga ttgttaaaca ttatcgaggg
    12481 tatacctcaa aacgttcatt acgtcgcatg acagacgaat atggaaattc tcatatggtt
    12541 attgatgaaa ccctacgtgg cagaatggaa acgaaactca tctctaagat tttatcgttc
    12601 gaaattaaat agtcccattt tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat
    12661 ttgggaggtt tgttattcca tcaaagcata ttgtgtgttc agtaggtttt gataggctaa
    12721 caagccaaac gttctttgac aaccaaataa aggcaatcga atacgtttcg gtaagaaaag
    12781 agctgatgaa ctaacacgcc atgacctaca aaattagtga gcgattcatg ttagtaattc
    12841 gaaagtaatc tttagcagga ttgctgccat gactttctta tcgtgataat gatactcccg
    12901 tatagccata gtccgagcgt tagaagcgtc acaggcaatg ggtacggcta catgagaacc
    12961 atgcagaagt gaaattcctg tgagctttgc tagagctgtt cgattgctaa gatattcttc
    13021 tcttgtcagt gtattattat aaaactattt gatataatta taggtgaata ttggagtttt
    13081 cctagcctat aattgggaaa ggagtaataa tgataacggt cacaaaaaag gatttagttg
    13141 agctaggata tggaacttct ttttcagcag acatcataaa aaaagcaaaa gaattgatga
    13201 ttacaaaagg gcatatctac tatcaatctc gaaagctaga tagagtccct cgtgaagctg
    13261 ttgaagaaat tctaggaatt cggttttcaa atgaaaacag gtctgagtag tttttgcaca
    13321 gtaaggagta aaaactatgg ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta
    13381 ttttcgtgca aatctaggtt atgattctaa agggaaaaaa attcaaaaat atcgtagtgg
    13441 attcactact aaaaaagaag caagagaaga atattcgaaa ctgctattga tgaaaccaga
    13501 agaattaagt gagaataagg ataaaatgac gtttaaacat tatatctttg aaattttcct
    13561 tccatggtat aagacacagg taaagctcag aacatatgaa aatcgcttac caactattaa
    13621 gaagcatttc tcttattttg ataaaatggc tgtttccgat attgagccta ttgatgtgca
    13681 aaattggcaa ctaaaattat caaagaagtg taagtcttct tatgtaagag ctgttcaagg
    13741 actattttca gttgctatgg atcgagcaat tgtccttggg ataaccacca caaatccatc
    13801 taagattatt ggcaatgtga aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga
    13861 atttgaaaaa gtaatctctc taatttacaa agaagattac tatcaacact ttttatttat
    13921 ctctctgtgg tttttattta tgactggaat gcgaattggt gaagctactg ccattcaatg
    13981 ggaagatatt gattttgact caggagtact aaccatcaac aaaacactct attacaagaa
    14041 tcaatcgaat tatagatttg ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt
    14101 agataaatgc acattaactt atttatccga atggaaggaa gtacaacaaa atctaattca
    14161 gacagacttt gtaatgagct ataacggtat tcctacccaa aaacatacac tatcatatgc
    14221 tattgaacgc tatgctaagc tggctggtat ccatcgaatc agaatccatg ggttacggca
    14281 ttctcatgct tccttgttaa tcagtatggg agaaaatcca cttatcatta aagatcgtct
    14341 aggacacgaa gatattgaga cgactttagg aacgtatgga catctttatc ctaacagtaa
    14401 tttcgaggta gctaacaaat taaatggagc catttccttt aaagaagcaa aacaaaatta
    14461 tgatacttca ccaaagaatc aatttactgt agactacttg cgaaacaaag aaatgaaaaa
    14521 agtgcaataa aagtgcagta aaaagataag aagagccaga aacccttggt ataagaggat
    14581 ttctagctct cctacaacta ttcccactcc actgttgctg gtggtttaga agtgatgtca
    14641 tacacgatac ggttgacgtg ggctacttcg tttacgatac gtacagagat tttttgcaag
    14701 acttcccatg ggatacgggc aaagtcagcg gtcattccgt cgattgaagt tacagcacgg
    14761 ataccgactg tgtagtcgta agtacgtccg tcacccataa caccgacaga gcggatccct
    14821 ggaagaacag tgaaatattg ccagatatca cggtcaagtc cagcagctgc gatttcttca
    14881 cgcaagatag catcagattc gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga
    14941 acacgaatac caagaccagg accagggaat ggctgacgcc aaacgattga atcaggcatg
    15001 cctaactgag tccctaattc acggacttcg tctttgaata atgtgtttaa tggttcgatc
    15061 aattcaaatt gcatatcttc tggcagtcca cctacattat ggtgagattt gatcgtctgt
    15121 gctgtttctg taccagattc gataacgtca gtatacaacg ttccttgtgc taggaaagag
    15181 acgccttctt caccagcaag ttttgttgct tcgtcatcaa aaacatatac gaattcattc
    15241 ccaatgattt tacgtttttg ttctgggtca gatacacctt tcaacttgtc taagaagcga
    15301 tctcttgcat cgactttgat gatgttcaag ccgaattttc cgcctagact ttccattact
    15361 tgttccgctt cgcctttgcg tagcagaccg tggtcaacaa agatagatgt caattggtca
    15421 ccgatcgctt tttgtaaaag aaccccaacg acactagaat caacgccacc tgaaagacca
    15481 agcaagactt ttttgtcacc aacttgttca cggatttttt ctacttgcat atcgataaag
    15541 ttatccattg tccagttacc ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat
    15601 tcgattccgt attcggaatg acgtacttct gcatggaatt ggataccata aaaattacgt
    15661 gttgtgtctt ggatagaagc aatcggacaa tctttgcttg ttccgactgt ttcaaagcct
    15721 tcaggaactt tagttaccaa gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt
    15781 tctttaaaca agacagcttg atcgcttgtt acttctaaca aggctttccc atattcgcgg
    15841 tttttagcag gctctacgga accgccaagt ttgtacgtga tcaattgcat accatagcaa
    15901 atccctaata caggaatacc taaattgaag atctcaggat cgatcccaaa tgcattctca
    15961 tcatatacac tgtttggtcc gccagaaaga atgattccct ttggattcat cgcttttact
    16021 tcttctgcag taatgcggtg gctcaataat tcggaaaaga ctccaagatc acgaatacgt
    16081 cttgtgatca attggttata ctgactaccg tagtctaata cgataatctt ttcaacgttc
    16141 gtcatatcgg caacgtttgt caaatttatt acccctatcc ctttaaattt tatctcaaaa
    16201 tagtcaaact attttaaaat gctaatattt tcgcatgaaa atcgaatcga tgatatgatt
    16261 ttcagtttta tggagaatta tatcggctct tcctcgtgta ggcaagatat attcctgtaa
    16321 atttttcaaa ttcacatttt tccatacatc ttttgccatt ttaaaggctt cttcacgatc
    16381 tccgatagca aaacgataat aataattctc tggattttgg aaagccgtat ccagtaacgc
    16441 accaaaacga tccaaatacc atttttcgat cagcacggga tcggcatcga catagacaga
    16501 aaaatcaaaa aagtcgctga cgtagatttg ctgattcgca ggtaattgaa gcgtattgat
    16561 cccctcaacg atcaagatat caggctgctg gatcaattcg tacttgtctg gaatgatatc
    16621 atacacatca tgtgagtaaa caggtacttt tatctcttcc tgaccagatt ttacacggtt
    16681 caagaaatcg atcagcatct ccatatcata actttcagga aatcctttac ggtccatgat
    16741 tccacgctct tttaatatac gatttggata aagaaatccg tcagtcgtga tcagctgaac
    16801 gtttcttcgt ttgaaggtac gagacaaaat cgtctgcagc agtctggctg ttgtactttt
    16861 tccaacggct acacttccgg cgatcccgat aataaaaggc ggtacttcta cgtatttatg
    16921 aagaaataaa cctttactca agaacaaaga ttcaaattct ttcatataaa gataaatcag
    16981 atgagcaaga ggtacataga tatctcgaac atcctgcatg gaaatgcggt cattcaaact
    17041 tttgatatta tctagttcat cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg
    17101 ccattcttct cttgccacac ggtagtagtt cattggatca ttcatattag cctcctgcgt
    17161 tctcccatca atggaactat tttaacacaa aataaacgca aaccccacca cttaagagag
    17221 attgccgatc tttttgtata atctttataa tagaaagaaa cggagtctta ttcttgactt
    17281 gcgcacggat acataaaagg atagactgtt gtcatgaaag aaatgagaag gatggaagac
    17341 atgacaaatc attactattc tgaacgccca gatacggcgc acgattttga acaatgggct
    17401 tttgagttaa aaggcagaac ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa
    17461 acagtggatt atggttcacg tgtcctgatt gatgctttcg attgggaaga gttacctgat
    17521 gaaggaaaga tcttagatgt gggatgcggg tatggaccga ttgggttagc tttggcattt
    17581 gcgacacagc ggtttgttga aatggtcgac attaatgaac gagcggtaag tctagcgcaa
    17641 ggaaacgcaa agcgtaacca aatcgaacat gttgatatcc atcaatcgaa tatctatgaa
    17701 gcagttcatg aagaaaccta tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa
    17761 aaagttgtac atgaaatttt gacaggcgca tatcctcggt tgaagaaggg cggtacactg
    17821 acaattgtga tccagaagaa acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca
    17881 tttggtaatg cagaaatcgt cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa
    17941 gaataactag ctgaaaaata gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg
    18001 gtacgtatca gttctttttt tgttgagaag tttttaaaaa caaagaacta ttttttcttc
    18061 aaaaagaaat agtaggaaca gagtcgttgt tttttacgat aaaataaata agatacagaa
    18121 cttgtataga aggctgccaa gtcggtagat aaaggaggtt tttatcaaaa aacatacttt
    18181 ccttttttct ctattctttt cttgggggtt ttggtacact gatagggaat ttagatagaa
    18241 gggagccaat aggatgaagc tgaccatcag agatatcgca gaaatggcag gcgtatcagt
    18301 aacaactgtc tcacagattt tgaataacaa aggaagcaga tttagtgata agacaagaaa
    18361 aaaagtgttg tcgatcgtga atgaacatca ttataagccc gattatttcg cttcaaatgt
    18421 gatcaatcga cattcaaaga ccatcggaat gatcgtccct gatgtcactg atttcttctt
    18481 ctccaaactg atcgaaggtg tagagagcta tttgaatcct ttgggctatg tgatcatgtt
    18541 atgcaattct cgtcatagcc aagaaaatga gatcaagtat ttacaagagc tttcccaccg
    18601 ctcagtggac ggaatcttgc tggccacacc aaatatttta cctgagaaat atgcattaga
    18661 cagcggtttt tatcaaaaaa tgccaatcat tttaatcgat cgcggattga atcgtcggga
    18721 taacggacgg cttatcgtaa aggaatatga gggcgcctat caagctgttt cgctgtttat
    18781 tgaaaatggc catacgaaga tcggcatgct gaaggaaacc actggatact atcaattaga
    18841 agaacgtttc aacggatatc gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa
    18901 atttgttgaa catggagagt tgactgtcca aggtggatat actgcatcaa aaaaattact
    18961 caaacataaa gatatcacgg ctattttttg cgggaatgat gccatggcga tcggatgcta
    19021 tcaggcgatc gatgaattgg gcaaaaaaat cccagaagat atctcagtca taggattcga
    19081 tggattaaag ctgtcagaat acatgattcc gcaattgacg acagtgcagc agccaagttt
    19141 tgatattgga ttttatgctg cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa
    19201 agttccgaac aaagtgttcg aaacaaaatt aattattcgt gagagcgtaa aagtattgac
    19261 aaagggctaa ctcggcggta tattgcttat gtaagcattt acaaaggcta gttagtgctt
    19321 tttttatttt tagtaaacca gtttactaaa gtggtttacc tttcctaaaa ataatgtata
    19381 ataccgaatg aagacaagga ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag
    19441 agggatggaa acgagctgtc gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat
    19501 ggaaagattc cagattatca agtcagtgca ttattgatgg caatttttta tcaagacatg
    19561 acaaatgaag aaatcacgaa cttaacattg gcgattgcta attctggtga tgtcatcgat
    19621 ttgagttctc ttgaaggaat aaaggtagat aagcattcga caggtggcgt tggtgacacg
    19681 accacattga ttttagcccc cctagtagct agtgtcggag tgacagtggc taaaatgtct
    19741 ggacgcggtc taggttacac aggtggtaca ctagacaagc tagaagcaat tcccggattt
    19801 cagatcgagc tttcggatga agaatttgta cgcattgtta atgaaagtaa agtagcggta
    19861 attggtcaat caggaaactt ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc
    19921 acagcgaccg ttgattctct tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg
    19981 gcaggagcag atgcaatcgt tttggatgtg accactggag acggtgcttt catgaagaac
    20041 atcgaagatg caagacgtct ggcaaagacc atgacgagca ttgggaaact ggctaaccga
    20101 gaaacagtgg cggttatctc agatatgtca gaacctcttg gcgaagctat cggaaatagt
    20161 ctagaagttg tcgaggcaat cgaaacatta caaggaaatg gtccagaaga tctagtagaa
    20221 atgtgctacg ccttaggaag ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat
    20281 gaagcaagaa cgttgcttca agaagctctg gaatcaggta aagcgttagc aaaattcaaa
    20341 gaaatgatcc aaaatcaagg gggagaccca gcgatcgtgg aacatccaga acgaattttg
    20401 actgcacgct acacaatgga gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg
    20461 gcaaatgaat taggaattgc tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa
    20521 gatattgatc acgctgttgg attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa
    20581 ggtgagtcac tattgacgat ctatagtaac gacgaagaaa tcacttctgt cattgaatta
    20641 ttatataaaa atattgaaat tggtgaatcg gcaatgaagc caacgttgat tcatgacatc
    20701 atcacagaat aaaggaaaaa agcggacttt accgttttaa agaaaagggg aaaagaaatg
    20761 gaactaaacc gaatgatcga ccatactatt ttgaaagcag acgcatctaa agaagctgtg
    20821 atgcaaatta tcgaagaagc aaaaaaatac catttttatt ctgtctgcat caatccagca
    20881 tgggttgcgc tagcgaaaga acaattacaa ggtgagccag tagccgtatg tacagtaatc
    20941 gggtttcctc taggagcaaa cacacccgag acaaaagcat ttgaaacaac agatgccatc
    21001 aataacggtg cagatgaagt cgatatggtc atcaatatcg gtgctttgaa atcaaaggat
    21061 gatcaacaag tccaaaaaga tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt
    21121 gtcaaagtta ttattgaaac atcgttatta gatagagaag aaatcatccg agcttgcgaa
    21181 atagcaaaag ctgcaggcgc tgattttgtc aaaacatcaa ctggtttctc aacaggcgga
    21241 gcaaaggtag aggatgtccg tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa
    21301 gcttccggcg gtatccataa cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca
    21361 agaatcggaa caagcgcagg agtagcaatc gtatctggtt caactggtga aggctattaa
    21421 ttttttcaaa ggagaagctc atggcagaac tcaaacaaga atggatcaac acagctattg
    21481 aagcgttaga taaagcctat gttccttatt cccattttcc ggtaggcgct tgtctcgtaa
    21541 cggaatcagg caagatttat caaggcatca acattgagaa tgcatcattt ggattaacga
    21601 attgtgccga gcgcacagct tttttcaaag cagtatctga aggagaaaaa acatttacgc
    21661 atctagtcgt agcggggcac acccccgatc ctatctctcc ttgtggtgca tgtcgacaag
    21721 taatggcaga attctgttcc cctgatatgc cggttacgct agtaggagac aatggtgtga
    21781 cgaaggcaac gaccgttcgt gcactgctgc cttatgcatt tactgaaaaa gatctataac
    21841 tcatgttcat tcagtcagct gactgttgaa aaataaaaca attattttag gaggctttac
    21901 agaaatgaaa aaagcaaaat tatttggttt aggtgctgtc gcactagcag cagggttatt
    21961 tttaggtgca tgcggcaaca acggctctac tgattcatcc ggtggcaaaa gctcatcaga
    22021 cacaacaact gcagcattga tcacagacac aggtggtgta gacgaccgtt cattcaacca
    22081 gtcagcttgg gaaggactag aaaaatgggg gaaagatcaa ggactttcac gaggaaatga
    22141 tggcttccaa tatttccaat cttcaaatga atcagattac atcccaaata ttgaccaagc
    22201 attgaatgct ggattcaaaa cgatttttgg tattggttat aaactaaaac cagcgatcca
    22261 agaacaagca acaaacaata caggtacgaa ctttgttatt atcgatgatg tcatcgatgg
    22321 attagataac gttgtatctg caactttcaa agacaacgaa gcttcttatc ttgcaggtgt
    22381 tgcagcagct tatacgactg aaacaaacgt agtcggcttt atcggcggtg taaaaggtga
    22441 agtaatcgat cgttttgatg ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt
    22501 aggaaaagaa atcaaagtat tgaatcaata tgctggtgac ttctctgctc cagataaagg
    22561 acgttcaatt gctcaaggta tgtatgcaca aaacgcagat atcatcttcc atgcttcagg
    22621 cggtacaggt aacggtgtat tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa
    22681 agtttgggtt atcggtgttg accgcgacca atcagacgaa ggcgaataca cattaaacgg
    22741 cgaaaagaaa aacttcacat tgacatctac actgaaagca gttggaacag tagtagaaga
    22801 tttggcacaa aaatcagcgg acggtaaatt ccctggcggc gaacacactg tttatggatt
    22861 gaaagaagac ggtgttggtc taacagaagg acaactttct gacgaagcga aaaaagcagt
    22921 tgatgaagca aaagaaaaaa tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga
    22981 aaactaaaac catttctata gccttgttag acacatgttt gacaaggcta tggtttgatt
    23041 agaacaagaa ttgttcattt atccaagctg ccttgtgaca gtttggataa gggaacaact
    23101 ccctgaagta atgtttgttt caaaagaaga acgctcacta agcgtgaatc ttagtcaatc
    23161 gttgccctag atagaaatcg ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca
    23221 tagaaatgca gaatattacc aaagaatttg gaacattcaa ggcaaatgac aacatcaatt
    23281 tacaagtaaa ggcaggagaa attcatgcgt tgcttggaga aaacggtgct ggcaaatcta
    23341 cattgatgaa cgtgctttcc ggattattag agccgacatc agggaaaatt ttgatgcgtg
    23401 ggaaagaagt aaagatcaca agtccgacca aagccaacca attagggatt gggatggtcc
    23461 atcagcactt tatgcttgtt gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg
    23521 agcctagtcg cgcagggatg cttgaccata aaaaagcacg aaaagagatc caaaaagttt
    23581 ctgaacagta tggattgtca gtcaacccag atgcttacgt tcgtgatatt tcagttggaa
    23641 tggaacaacg ggtagaaatt ttaaaaacac tttaccgagg agcagatgta ctgatttttg
    23701 atgagccgac agctgtattg acccctcaag aaattgatga attaatcgtg atcatgaagg
    23761 aattagtcaa agaaggcaag tcgatcattt tgattacgca taagttagat gaaatcaaag
    23821 cagtagctga ccgctgtaca gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta
    23881 aagacgtaac ctcacaacaa ttagccgata tgatggttgg aagaacggtt tcattcaaaa
    23941 cgatgaaaaa agaagcgaag cctcaagaag tcgttttgtc tattgaaaat ctagtggtga
    24001 aagaaaatcg tggattagaa gccgtgaaaa acttgaactt agaggttcgt gctggcgaag
    24061 tacttggtat cgctggaatc gatggaaacg ggcagtcgga gttgatccaa gctttgactg
    24121 gtttgcgaaa ggcagaaagc ggacatatca agctaaaagg ggaagacatc actaataaaa
    24181 aaccccgaaa aatcactgaa catggtgtag gacacgtgcc agaagaccgt cataaatacg
    24241 ggttagtcct agatatgaca ttatctgaaa atatcgcact acaaacctat catcaaaaac
    24301 cttacagtaa aaacggcatg ctgaattatt cagtaatgaa tgaacatgcc agagaattga
    24361 tcgaagaata tgatgttcga acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg
    24421 gaaatcagca aaaagcaatt atcgctcgtg aagtcgaccg agatcctgat ctgttgattg
    24481 ttgcaaatcc aactcgtgga ctggatgtag gtgcgatcga atttattcat aaacgtctga
    24541 tcgaacaaag ggacaaatac aaagcagtgt tactgatcag tttcgaatta gaagaaattt
    24601 taaatgtttc ggatcgtatt gctgttatcc atgaaggaga aattgtcggt atcgttgatc
    24661 cgaaagaaac atctgaaaat gaattaggtc tattgatggc gggctatacc ttagaagaag
    24721 cacgaaaaga gttagaaaag ggggctggcg cacatgaatg atcgcagcga aaaattccgt
    24781 aatttattag tgcctgtact ttcagttgtt ttaggattga tcttaggcgc gatattgatg
    24841 gctgcttttg gttttcagcc catccaaggt tattcttcta tgctagatgc agcccttggt
    24901 tcgcaaagag ggataggtga aacgttaagg caagcaacgc cgttgatttt tacagcatta
    24961 ggtttttctg tagctaactc agctgggttt ttcaatattg ggctttctgg acaagcactt
    25021 tgcgggtgga tcgtcagtgt ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt
    25081 ttgcttccgt tatgcgtgat cttaggtgcc ttagctggtg cgatggcagc ggctgttcca
    25141 ggtttacttc gagcattttt tggaacaagt gaagtgatcg taacaatcat gatgaactat
    25201 atcttattat acgtaagcac atttgcttta catgaagtga tgcctgaaag ctatcgctca
    25261 agtttggact cttccaatat gatcagtcaa aatgcctcac tgcggttacc atggttaaca
    25321 caagcttttg gcggctctcg cgtaaactca ggattatttt tagcaatcat cgcgcttatt
    25381 ttcgtttggc tgctgatgaa gaaaacgact ttaggatttg aaatccgttc tgttggattg
    25441 aatccttttg cttctgagta tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg
    25501 gtcatttctg gcgctttggc agggcttggc ggtgtggttg aaggattagg aacgtatcaa
    25561 aacttctttg ttcaaacgac ttcgctatct attggatttg acggaatggc tgtttccttg
    25621 cttggttctg gatcagcaat cggtattctt ttatcagcga tcttgttcag tattttgaag
    25681 atcggtggac ttggcatgca gacaggagca ggtgttccgt ttgagatcgt caatgtatcg
    25741 attgctttga ttatcttctt tgtcgggatc aacttcctga ttcgtttcgt catggctaaa
    25801 gtcttccaag ggaaaaaaca agaagagatc gttgcgacaa tagaatcgaa accaacgaat
    25861 caaaaccatg aagggggcga actataatgt cgactatcga attaattgcc ttgatcctta
    25921 cttctacact ggtttattca acaccgctaa ttctaacgtc actaggcgga actttttctg
    25981 aacgaagcgg gatcgtaaac gtagggctag agggaatcat ggtcatgggt gcttttagtt
    26041 ccatcgtttt caacttgacg atgagtccat cacttggttc tatgacacca tggatcgcta
    26101 ttttagtggg tggcgtcgta ggtatcctat tttctctgat tcacgcagta gcaacagtca
    26161 gtttgcgtgc tgaccatatt atcagcggga cagtaattaa tctgatggca cctgctttag
    26221 gtgttttctt gatcaaagca tggtatggta aaggtcaaac tgataacatc acgcagaatc
    26281 tggggtattt ttctttccca ggattagcag atattccggt cattggacca attttcttta
    26341 aaaatacatt cttacctgct tattgtgcta ttttgattgc tgtcctagct tgggtgatca
    26401 tcttcaagac gaaattcggc ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg
    26461 acacattagg tatcaatgta tatgctatgc gttattcagg cgtactgatt tctggcttgt
    26521 taggcggtat gggcggtgct gtttttgctc agtcgatttc cggtaacttt tcagctggta
    26581 cgatcgttgg acaagggttc atctccatgg ctgctatgat ctttggtaag tggaatcctt
    26641 taggggcaat gggtgcctca ttgttctttg gttttgctca aagcttgagt atcattggcg
    26701 cacaattacc agttatttct tcgattccgc cagtattgct gcaaattgct ccatatttgc
    26761 tgacaatcat tgtgctggtt gttttcttag ggaaagcttc tggtccaaaa gcaaacggga
    26821 agaactatat caaatcaaaa taatctttta gcgaataaaa agggtgggtt acgggaagtg
    26881 tttttcgtct cgcccttttt gttttaaagg caataaaaat aaaaagaaag aaggaataat
    26941 catgtttaaa cgcgtacatc tgatcgtcat ggattcagta ggaattggag aagcaccaga
    27001 tgccgataag ttcggtgatg ttggttctga tacattagga cacattgcga aagaagcagg
    27061 attgaacgtt ccacatttag agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt
    27121 ggaatcagtg aaagatcatc aaggctatgc gacgaaatta gaagaagttt ctgtagggaa
    27181 agatacgatg acaggccact gggaaatcat gggattgaat atcaaaactc catttcgtgt
    27241 gtttcctaat ggattccctg atgatctgat caaacaaatc gaagaatttt ctggccgtaa
    27301 agtcgtatgt aacaaacctt atagcggaac agctgttatt gacgattacg gtgaacacca
    27361 aatgaaaaca ggcgatttaa ttgtatatac ttctgccgat cctgtccttc agatcgcggc
    27421 acatgaagaa atcattcctt tagaagaatt atatcggatt tgccaattcg ttcgtgatat
    27481 aacaaaagat gatccgtata tgattggccg aatcattgca cgtccgtacg tgggtgaacc
    27541 aggagacttc acacgaacaa gcaatcgcca tgattatgcg ttgaatccat tcggacacac
    27601 agtccttgat tctttgaaag aagcaggaaa ggacgtgatt gctgttggaa agatcaacga
    27661 tattttcaac ggacaaggaa tcactgaatc tgtccgcacg aaaagcaata tggatggcgt
    27721 tgaccagctg ctgaaagtta tgaaaaacga tttttcagga atcagcttta ccaatttagt
    27781 agattttgat gcattatacg gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga
    27841 agaatttgac ttacgaattc cagaattgct tgaagcgatg gaagaagacg acttgctaat
    27901 gattacagcc gatcacggaa acgatccgac gtttactggt accgatcata cgcgagaata
    27961 tgtaccgtta ttggtttaca gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata
    28021 ttattcagat atttcagcaa cgattgctga gaactttagt gtttcttcga ctgaaaatgg
    28081 cgaaagcttc ttgaaattat taaaataatc tttttaaata ggagaaagca aatgaccaag
    28141 ttaagcgaga tgttacaaga aacgacccag tttatccttg ataaaggagt aaaagaaatt
    28201 gattttggtt tgattctagg ttcaggatta ggtgaattag cagatgaaat cgaagatgct
    28261 gtaatgattt cttatgatga gatcccgttc tttcctgttt cgacagtagt tgggcatgca
    28321 ggaaagctag tttatgggtc tctggcaggc aagaaagtgt tagccatgca agggagattc
    28381 cactactatg aaggtcattc catgcaaaca gtgacctatc cagtacgtgt gatggccgga
    28441 ctaggggccc attcagtcat tgtgactaat gcatgtggtg gtgtcaacga atcatttgta
    28501 ccaggagatt tgatgctgat taccgatcat atcaatttta ctggtgaaaa tcctttgatc
    28561 ggtccgaatg aagatgcaat gggccctaga tttccagata tgagtcaagc ttatacgcct
    28621 gactatcaag caacagcaaa agcagcagca gaaaaactag gtttggatct taaagaaggc
    28681 gtatatatgg gctattctgg accaacttat gaaacgcctg cggagatccg aatgtctcgg
    28741 acaataggag ccgatgcagt agggatgtcg actgttccag aagtcatcgt agcagctcac
    28801 agcggattaa aagtgttagg aatctcttgt attaccaact tagcagcagg tatgcaagcg
    28861 aatctgagcc atgaagaagt tgtagaaaca actcagagag tcaaacaatc ttttaaagca
    28921 ttggttaaag aaacattgac attattgtaa taggaggaac aataaaatga gtgtgcatat
    28981 tgaagcgaaa gaaggcgaga tcgccgataa gattttactt ccgggagatc ctttgcgagc
    29041 taaatatatt gcggagactt ttttggaaga tccagtttgt tacaatcagg tacgcggtat
    29101 gttaggcttt accggcaaat ataaaggaga acgtatttct gttcaaggaa caggaatggg
    29161 aatgccatca gccaccatct atgcccatga attgatccag tcgtatggtg taaagaaatt
    29221 gattcgagta ggtacatgcg gtgcattgtc aaaagacgtc catgttcgtg atttagtact
    29281 agcacaaggt gcagcgacca gttcttcgat gatcgaaaaa aatttccaag cctttcattt
    29341 tcctccaatc agtgatttca atctattact gaaagcatat gagatagcta aagaaaaggg
    29401 ctatacggtt catgtaggaa atgttctttc ggaagattct ttctacaaag atgatttgac
    29461 agagacgttc caattagctg aacttggcgt tttaggagtg gaaatggaag cagcggctct
    29521 ttattactta ggcgcgaaat accatgtgca gacattaagt ttgatgaccg ttagcgacca
    29581 tttgatcacc ggtgaagaaa caacagctgc agaacgtcaa tctacgttca atgaaatgat
    29641 cgaagtagga ttggaaacag cgatagcaga ataagaagga aatgaaaaag ctgccctgca
    29701 ttaagtaata agaacatcca agcaaaaata gctcctcatg tttttgactt ggctggactt
    29761 attattgcaa atgtaa
//
LOCUS       DACENJ010000012        29103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.012, whole genome shotgun sequence.
ACCESSION   DACENJ010000012 DACENJ010000000
VERSION     DACENJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.012"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1030)
                     /locus_tag="IW748_000377"
     CDS             complement(<1..1030)
                     /locus_tag="IW748_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1103309.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IW748_000378"
     CDS             complement(1112..1801)
                     /locus_tag="IW748_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1103310.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IW748_000379"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IW748_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1103311.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IW748_000380"
     CDS             complement(2328..2831)
                     /locus_tag="IW748_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1103312.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IW748_000381"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IW748_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1103313.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IW748_000382"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IW748_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1103314.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IW748_000383"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IW748_000383"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1103315.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IW748_000384"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IW748_000384"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1103316.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IW748_000385"
     CDS             complement(6443..7462)
                     /locus_tag="IW748_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1103317.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IW748_000386"
     CDS             complement(7635..8522)
                     /locus_tag="IW748_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1103318.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IW748_000387"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IW748_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1103319.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IW748_000388"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IW748_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1103320.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IW748_000389"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IW748_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1103321.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IW748_000390"
     CDS             9753..10397
                     /locus_tag="IW748_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1103322.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IW748_000391"
     CDS             complement(10453..10956)
                     /locus_tag="IW748_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1103323.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IW748_000392"
     CDS             11553..12518
                     /locus_tag="IW748_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1103324.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IW748_000393"
     CDS             complement(12617..13789)
                     /locus_tag="IW748_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103325.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IW748_000394"
     CDS             complement(13776..14921)
                     /locus_tag="IW748_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103326.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IW748_000395"
     CDS             complement(14935..15867)
                     /locus_tag="IW748_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103327.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TFLQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IW748_000396"
     CDS             15984..17063
                     /locus_tag="IW748_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1103328.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IW748_000397"
     CDS             17060..17689
                     /locus_tag="IW748_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1103329.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IW748_000398"
     CDS             complement(17799..18026)
                     /locus_tag="IW748_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1103330.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IW748_000399"
     CDS             complement(18129..20717)
                     /locus_tag="IW748_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1103331.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IW748_000400"
     CDS             complement(20737..21396)
                     /locus_tag="IW748_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1103332.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IW748_000401"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IW748_000401"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1103333.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IW748_000402"
     CDS             complement(22169..23035)
                     /locus_tag="IW748_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1103334.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IW748_000403"
     CDS             23144..23785
                     /locus_tag="IW748_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1103335.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IW748_000404"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IW748_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1103336.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IW748_000405"
     CDS             complement(25226..26125)
                     /locus_tag="IW748_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1103337.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IW748_000406"
     CDS             complement(26127..26924)
                     /locus_tag="IW748_000406"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1103338.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IW748_000407"
     CDS             complement(26902..27606)
                     /locus_tag="IW748_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1103339.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IW748_000408"
     CDS             27737..28312
                     /locus_tag="IW748_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1103340.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..>29103
                     /locus_tag="IW748_000409"
     CDS             28430..>29103
                     /locus_tag="IW748_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1103341.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8562 a         6169 c         5232 g         9140 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagaatgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taa
//
LOCUS       DACENJ010000013        28520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.013, whole genome shotgun sequence.
ACCESSION   DACENJ010000013 DACENJ010000000
VERSION     DACENJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.013"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            201..689
                     /gene="purE"
                     /locus_tag="IW748_000410"
     CDS             201..689
                     /gene="purE"
                     /locus_tag="IW748_000410"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1103342.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            673..1806
                     /gene="purK"
                     /locus_tag="IW748_000411"
     CDS             673..1806
                     /gene="purK"
                     /locus_tag="IW748_000411"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1103343.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1819..3114
                     /gene="purB"
                     /locus_tag="IW748_000412"
     CDS             1819..3114
                     /gene="purB"
                     /locus_tag="IW748_000412"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1103344.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3294..3671)
                     /locus_tag="IW748_000413"
     CDS             complement(3294..3671)
                     /locus_tag="IW748_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103345.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            3927..4655
                     /locus_tag="IW748_000414"
     CDS             3927..4655
                     /locus_tag="IW748_000414"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1103346.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            4655..4909
                     /gene="purS"
                     /locus_tag="IW748_000415"
     CDS             4655..4909
                     /gene="purS"
                     /locus_tag="IW748_000415"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1103347.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            4911..5582
                     /gene="purQ"
                     /locus_tag="IW748_000416"
     CDS             4911..5582
                     /gene="purQ"
                     /locus_tag="IW748_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1103348.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            5583..7805
                     /gene="purL"
                     /locus_tag="IW748_000417"
     CDS             5583..7805
                     /gene="purL"
                     /locus_tag="IW748_000417"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1103349.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            7790..9229
                     /locus_tag="IW748_000418"
     CDS             7790..9229
                     /locus_tag="IW748_000418"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1103350.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            9261..10304
                     /locus_tag="IW748_000419"
     CDS             9261..10304
                     /locus_tag="IW748_000419"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1103351.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            10301..10879
                     /gene="purN"
                     /locus_tag="IW748_000420"
     CDS             10301..10879
                     /gene="purN"
                     /locus_tag="IW748_000420"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1103352.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            10876..12411
                     /gene="purH"
                     /locus_tag="IW748_000421"
     CDS             10876..12411
                     /gene="purH"
                     /locus_tag="IW748_000421"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1103353.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            12427..13701
                     /gene="purD"
                     /locus_tag="IW748_000422"
     CDS             12427..13701
                     /gene="purD"
                     /locus_tag="IW748_000422"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1103354.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            13823..14737
                     /locus_tag="IW748_000423"
     CDS             13823..14737
                     /locus_tag="IW748_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1103355.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(14870..16435)
                     /locus_tag="IW748_000424"
     CDS             complement(14870..16435)
                     /locus_tag="IW748_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1103356.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(16428..17879)
                     /locus_tag="IW748_000425"
     CDS             complement(16428..17879)
                     /locus_tag="IW748_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1103357.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(17893..18909)
                     /locus_tag="IW748_000426"
     CDS             complement(17893..18909)
                     /locus_tag="IW748_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1103358.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            19129..20217
                     /locus_tag="IW748_000427"
     CDS             19129..20217
                     /locus_tag="IW748_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1103359.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            20271..21797
                     /locus_tag="IW748_000428"
     CDS             20271..21797
                     /locus_tag="IW748_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103360.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            21799..23004
                     /locus_tag="IW748_000429"
     CDS             21799..23004
                     /locus_tag="IW748_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1103361.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            23261..24238
                     /gene="guaC"
                     /locus_tag="IW748_000430"
     CDS             23261..24238
                     /gene="guaC"
                     /locus_tag="IW748_000430"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1103362.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            24502..25926
                     /locus_tag="IW748_000431"
     CDS             24502..25926
                     /locus_tag="IW748_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1103363.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            26387..27496
                     /gene="pdhA"
                     /locus_tag="IW748_000432"
     CDS             26387..27496
                     /gene="pdhA"
                     /locus_tag="IW748_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1103364.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            27499..28476
                     /locus_tag="IW748_000433"
     CDS             27499..28476
                     /locus_tag="IW748_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1103365.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         9216 a         4874 c         6442 g         7988 t
ORIGIN      
        1 tccttgtgac cgaggagttg gcaggagaac accgtttatc cataaaaagg aatgtgacct
       61 aacttttgtc acattccttt tttttatctt taataaacac gaacaataaa atgtttttct
      121 ttgttaacat tcgaaatttc tttgacatca aaaaaagtga ttgttaaaat atgaatgtag
      181 aaaaactggg aggaaaaaca atgacagcaa aaatctctgt gattatgggg agtacttcag
      241 attgggaaac aatgaaagaa gcatgtcaaa tattggatga attcggtgtg tcttatgaaa
      301 aaaaggtcgt ttctgctcat cgaaccccag atttgatgtt tgaatatgca caaaacgctc
      361 gaaaaaaagg aatcaaaatc attattgcag gagcaggggg agccgcgcat ctgccgggta
      421 tggtcgcatc aaaaacaact ttgccggtta tcggtgtacc ggtccaatca cgaacattaa
      481 atggtttgga ttcgcttctt tcgattgtcc aaatgcctgg aggagtacct gtagctacta
      541 cagcaatcgg aaaagcggga gcaattaatg ctggactact tgcgatacag atgctttcca
      601 tgtatgattt agaattggag gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg
      661 ttatagaaag tagtgatcaa cttggttaaa cctttgttgc ctggttcaac gatcgggatc
      721 gtcggtggcg gtcaattagg tcgtatgatg gcgattagtg caaaagaaat gggctttaaa
      781 gtaggtgtgc tggatcctgt gagcgattgt ccagccgcac aagttgcaga ttggcacatt
      841 gaaagtgtct atgatgacac ctttgcgctg aaagagcttg ccagaagaac agatgtcatt
      901 acctatgaat tcgaaaacgt cagtgtagag gcgttaaatg cgattttacc catgtcattt
      961 attcctcaag ggacagactt gcttgcgatt acgcaagacc gcctgttaga aaaatctttt
     1021 ttggaaacga ataacatcgt gattgctcct tatgcaacga tcgtcagtcc gacggatatc
     1081 caagatgcga ttgacgggat cggctatcct tgtgttttga aaaccactcg aggcggctat
     1141 gatggcaagg ggcagtatgt tttgaaaagt cgggcggatc tagctcctgc catggacctt
     1201 ttaagagaag gaacctgtgt tttagaagca tggatcccgt ttgaaaaaga gatttcgatc
     1261 atggtggcag gaaatggtca aggagatttc acgacttttc cggtcgtgga aaacatccac
     1321 cacaacaata ttcttcatga aacgattgct ccagccgcga ttgatcagga tgtgatcgag
     1381 gaggctgagc gtattgcccg tgtgattgca gaagcagtga gcctttcagg tgttttaggg
     1441 gtagaaatgt ttttaacaaa aacaggcggg ctatatgtga atgaattagc tcctcgtcct
     1501 cacaattctg gacattattc tattgaagct tgttctatga gtcagttcga tgcccatatt
     1561 cgaggtattt gtggttggcc attaggagaa aaagtacgtt tgataagcaa tgcagtaatg
     1621 gtcaatgttt tgggagaaca actgctggaa agctatcgct tgattgagaa taagccagac
     1681 tggcaattcc atttctatgg aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc
     1741 actatcttga cagaagacat ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg
     1801 gattagagga gaaaaaacat gttagaacgt tatacacgcc ctgaaatggg gaatatctgg
     1861 acggaagaga acaaataccg tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg
     1921 gcagaacttg gtgaaatccc gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt
     1981 gatatcgatc ggatcttgga aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca
     2041 cgagcggttt ctgaaacatt gggagaagaa agaaaatggg tccattatgg tctaacaagt
     2101 acggatgtcg tagatactgc ttatggctat ctcatcaagc aagcaaatga tattttacgg
     2161 gaagacttga aacgttttac ggaaatcatt ggcgaaaaag caaaagaaca caaatataca
     2221 gtgatgatgg gtcggacaca cggtgtccat gctgaaccaa ctacttttgg attgaaactt
     2281 gccacatggt attcagaaat gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga
     2341 gtagaagcag gaaaaatctc aggagcagtg ggaacgttcg caaatatccc gccatttatt
     2401 gaagagtatg tatgtaaaca attggggatc cgtccacaag aaatttctac tcaagttctc
     2461 cctcgggatc tacatgctga atatttctca gcgatggcgc ttattgctac aagtattgaa
     2521 cgttttgcaa cagagatacg cggattgcaa aaatccgaaa cacgtgaagt agaagaattt
     2581 tttgcgaaag gtcaaaaagg ttcttctgct atgccgcaca aaaggaatcc gatcggttca
     2641 gaaaatatgg ctggtctagc cagagtggtg cgcggacaca tggtcactgc ctatgaaaat
     2701 gtcacattat ggcatgaaag agatatttct cattcttcag cagaacgaat catcttgcct
     2761 gataccacga ttttattgaa ttacatgtta aatcgttttg gcaatatcgt taaaaatcta
     2821 acggtctttc ctgaaaacat gaagcggaat atgtctgcaa catttggttt gatttatagc
     2881 cagcgcgtta tgctgaaatt gatcgataag ggaatgacaa gagaagaagc ttatgatctt
     2941 gttcagccaa aaactgcgta cgcttgggac aaccaagtgc agttccgccc gctgttagaa
     3001 gaagatccaa ccatcacatc ggtattatct aaagaagaaa tagacgatgc ctttgattat
     3061 catcatcatt tgaaaaacgt tgacgtgata ttcaaacgag taggtttgga ataaagaaga
     3121 gtgagttgca aaattcaatt aaaatagaat agcaaaaata accaaaaaag aagcagatcc
     3181 gttagcggtt ctgcttcttt tagtttatta tattttctta tcaataatag gaacctgatt
     3241 ttaaaagcgg ctacctcttt accgtgcctc tttacataca aaaaatagag gaattaagcg
     3301 taaatggctt ggctattata actaaaatgc tggttatgga gatgttgcag caagttttcg
     3361 agaaaagcgt attccatctc ttgtccattg aataagatct ggatacgata atgatttttg
     3421 aaacgcttct gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc tgtctgataa
     3481 agaaaagtcc ttttacggca ttcagcacag cgcttggttg ctcggcggat ttttcggaat
     3541 gtataaaaac tagtatcttt attgcataaa taaacgtctt tgatcgacag caaatcatta
     3601 tatagacagg aacgttcttc agctgttaat tctgataaaa aaagctgacc ttgttttatt
     3661 gcatcttcca tcggcaagtg cctcctcgaa cccattatcc aaatttttag ttaattctaa
     3721 tataccatca ttaagtataa atgtaaacgc ttgttgatgg tataagattt tcgtttctga
     3781 taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg ttgatttata
     3841 aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt
     3901 aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag
     3961 ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag
     4021 caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc
     4081 aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg
     4141 aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg
     4201 ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag
     4261 aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat gatccattta
     4321 tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc agtgagctga
     4381 aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg
     4441 ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg
     4501 aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag
     4561 acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc
     4621 tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac
     4681 ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat
     4741 gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga
     4801 tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt
     4861 aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg
     4921 cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga
     4981 tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg
     5041 gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc
     5101 ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg
     5161 gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt gtactgagac
     5221 gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg
     5281 cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag
     5341 gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct
     5401 ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag
     5461 cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat
     5521 cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat
     5581 agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg
     5641 aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc
     5701 ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa
     5761 attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg
     5821 gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa
     5881 gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa
     5941 tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt
     6001 tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg
     6061 gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt
     6121 gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata
     6181 tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag
     6241 gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac
     6301 agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt
     6361 gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag
     6421 caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga
     6481 cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg
     6541 aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac
     6601 tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat
     6661 atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag
     6721 atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt
     6781 tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc
     6841 aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca
     6901 atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg acgaataaag
     6961 cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag
     7021 gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg
     7081 cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat
     7141 cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag
     7201 gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg
     7261 gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag
     7321 gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa
     7381 agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga
     7441 gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact
     7501 gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga
     7561 tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg
     7621 tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg
     7681 ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga
     7741 tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga
     7801 agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc
     7861 tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat
     7921 cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt
     7981 attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg
     8041 ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca
     8101 tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa
     8161 agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca
     8221 tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga
     8281 agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga
     8341 cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc
     8401 ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg
     8461 tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca
     8521 gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg
     8581 gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt
     8641 aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc
     8701 ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac
     8761 atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt
     8821 acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac
     8881 gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag
     8941 gattgcttct cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa
     9001 agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc
     9061 tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc
     9121 gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta
     9181 tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa
     9241 gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag
     9301 ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta
     9361 tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag
     9421 agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag
     9481 cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag
     9541 cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt
     9601 tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc
     9661 tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag
     9721 ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc
     9781 caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg
     9841 ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga
     9901 aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc
     9961 cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag
    10021 aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc
    10081 cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt
    10141 atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg aaagtaaaag
    10201 aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg
    10261 ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc
    10321 ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga
    10381 atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac
    10441 tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta
    10501 tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg
    10561 atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat
    10621 ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc
    10681 tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc
    10741 aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga
    10801 aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa
    10861 tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta
    10921 gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag
    10981 catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct
    11041 gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga
    11101 agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac
    11161 tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata
    11221 gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa
    11281 aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa
    11341 ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt
    11401 cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa
    11461 aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa
    11521 aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca
    11581 gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct
    11641 gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat
    11701 ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca
    11761 gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca
    11821 gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa
    11881 gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag
    11941 gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat
    12001 caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact
    12061 ccagcagaag caaaagcatt agtgtttgct tggagagctg tcaaacatgt gaaatctaat
    12121 gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga
    12181 gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta
    12241 gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata
    12301 aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat
    12361 aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc
    12421 ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa
    12481 cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca
    12541 aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt
    12601 gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg
    12661 atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct
    12721 ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca
    12781 gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt
    12841 gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc
    12901 acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat
    12961 agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg
    13021 tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt
    13081 gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt
    13141 tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg
    13201 aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa
    13261 ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt
    13321 tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta
    13381 gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac
    13441 cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt
    13501 tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat
    13561 ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta
    13621 atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat
    13681 agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa
    13741 tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt
    13801 atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc
    13861 agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat
    13921 tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg
    13981 tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct
    14041 ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca
    14101 tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt
    14161 acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga
    14221 attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat
    14281 cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt
    14341 gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat
    14401 cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg
    14461 gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga
    14521 attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc
    14581 ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt
    14641 agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta
    14701 cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa
    14761 gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg
    14821 tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat tagttatttt
    14881 tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt tttttcttaa
    14941 ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata tctgaaatac
    15001 gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct gtcagataat
    15061 cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag ggacttaatg
    15121 caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt tctttgataa
    15181 aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt gagggcagag
    15241 cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg tcggcgctga
    15301 ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt
    15361 tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat cgtgttcgag
    15421 ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg tttttcgata
    15481 atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga agtttcgctt
    15541 cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg gtttcaagta
    15601 tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg acaggaccaa
    15661 tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac gtttgggcta
    15721 cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa aatttgatga
    15781 atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa aacttcaaat
    15841 aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct gctaataata
    15901 cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc aatgtttcac
    15961 tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc aattcgaaaa
    16021 caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt actgcttttt
    16081 ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca tctgcctgta
    16141 cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat atgagacgag
    16201 tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt
    16261 ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt gccatctcac
    16321 catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca gttatttgtt
    16381 cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta tacatgtttg
    16441 tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt tccttctcgg
    16501 tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag gagacgcttg
    16561 tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt ggctaactct
    16621 ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat aactttgaat
    16681 tggatcatct catcaatttt gcatgtggta atacgaataa atcctttgtg acggacgtac
    16741 ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt taaaatagaa
    16801 acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc gtatcttatt
    16861 tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg aatccagtct
    16921 tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat agtcagagct
    16981 tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt ataaataaaa
    17041 tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg gtattgtttc
    17101 aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat gctagcggta
    17161 agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa
    17221 agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct ctctaaaaag
    17281 cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc cactattgtt
    17341 aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc gatctccaca
    17401 cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac ctgattgatg
    17461 atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag ctcgttttga
    17521 taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat ttctttaagt
    17581 gtatttgtat tatttttgat ctctcgaata tcattcctaa gttcttctag gatattttcc
    17641 tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact ttccaaaact
    17701 ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat cgtctgttga
    17761 taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat aacagcaaat
    17821 agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg
    17881 atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac
    17941 agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata
    18001 acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt
    18061 gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat
    18121 caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat
    18181 ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg
    18241 tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt
    18301 catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga
    18361 ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc
    18421 taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc
    18481 ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt
    18541 cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa
    18601 tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc
    18661 aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag
    18721 aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca
    18781 gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt
    18841 attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac
    18901 acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg
    18961 aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga
    19021 caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct
    19081 ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa
    19141 tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt
    19201 ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa
    19261 tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt
    19321 tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa
    19381 aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta
    19441 actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta
    19501 ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta
    19561 tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg
    19621 attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg
    19681 atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg
    19741 agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat
    19801 aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat
    19861 ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa
    19921 cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc
    19981 ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa
    20041 atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga aacttatgat
    20101 aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat
    20161 tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag
    20221 gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt
    20281 gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg
    20341 tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa
    20401 aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa
    20461 tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca
    20521 tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt
    20581 ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa
    20641 catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga
    20701 ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc
    20761 tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga
    20821 gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga
    20881 atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga
    20941 cactagaaaa tgaaatgatc agcgaagagc atattattcg cggaatggtg ggcagagatt
    21001 tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag
    21061 actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc
    21121 atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat
    21181 tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg
    21241 aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg
    21301 tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga
    21361 cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa
    21421 ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg
    21481 tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac
    21541 aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg
    21601 agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt
    21661 ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta
    21721 ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga
    21781 aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa
    21841 ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta
    21901 gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat
    21961 ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta
    22021 gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca
    22081 attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt
    22141 cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata
    22201 ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt
    22261 ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat
    22321 ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc
    22381 atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta
    22441 tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta
    22501 ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt
    22561 ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt
    22621 tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc
    22681 acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct
    22741 gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg
    22801 gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt
    22861 gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta
    22921 gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat
    22981 aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa
    23041 gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg tttgtataga
    23101 ctaaaaataa tatatgtttt tttaccattc gtataaattc tctttttgct cttgattttt
    23161 ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag ccttgattaa attaataatg
    23221 ttcgtgtttt gctggatgtg aacatcagga ggataatgta atgaaagtat ttgattacga
    23281 agatattcaa ttaattccta ataaatgtat cgtaaatagc cgttctgaat gtgatacaac
    23341 ggtcacatta ggtaaacata cattcaaaat gccagtagtg cctgccaaca tgcagacgat
    23401 tattgatgaa acaatcgcag aattcttagc ggaaaatggt tatttctata tcatgcatcg
    23461 gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg gattgattac
    23521 ctcgatcagt gttggcgtca agaaagagga atattccttt atagagaagt tggcagaaga
    23581 atcgctaaat cctgattata tcactatcga catcgcgcat ggacatgcta actcagttat
    23641 tgatatgatt cagcatatta aaaaatattt gcctgaaact tttgtgattg ccggtaatgt
    23701 tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa caaaagtcgg
    23761 gattggtcca ggaaaagtat gtattacaaa aatcaagaca ggctttggga ctggaggctg
    23821 gcaactggca gctcttagat ggtgtgcgaa agcagctcgc aagccgatca tcgctgacgg
    23881 tgggatcaga acacatggcg atattgcaaa atctgtccgt tttggtgcga caatggtcat
    23941 gattggttca ttgtttgccg gtcacgagga atctcctggc gaaacaaaag tcgaaaatgg
    24001 aatcgtgtat aaagaatact ttggttctgc ttcagaattc caaaaaggcg aaaaacgaaa
    24061 tgttgaagga aagaaaatct ggatccaaca caaaggatct ttgaaagata cactagtgga
    24121 aatgcaacaa gaccttcaat catctatttc ttatgctgga ggaagagatc tggaagccat
    24181 tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt aatggagaca caatctaaaa
    24241 aataaataaa atattaggat gttgtgaaaa agttgtcctg cattaagtaa taagaacagc
    24301 caaacaaaaa tagttcttta tatgttttgt ttggctggaa ttattatcgc agatacaagc
    24361 ttttgaacat cattattcag aataagggac tgtgataaga tttttgtcac agtcccttat
    24421 gttttgcaaa aaaagttttt cttttttctc gtaaattcgg taaaatcaaa agaaaaaata
    24481 gacaatagaa aagagaagct tatgtgggaa aaagtagaga aaaaacaagg accgatttat
    24541 cgtcaaatta tggaacagat tatgagagaa atcgaaaacg gaaggatcaa tcctggaagt
    24601 caacttgaat cagaacgaaa actagggcaa ctttttggtg tgaatcgttc caccgttgtc
    24661 catgcattgg aagagcttag ggagcttggg gtactaacga gcaaacgtgg cagcggatgg
    24721 tttgttaatc agactgaatg ggggaaattc gctgttccaa gaatcgattg gcggcaaatg
    24781 atctctcctc gttatgagca gacggactgg tatgagcaaa aaataagaga agcaaagcag
    24841 atccaaaaaa catcgtttct tgatttatac gctagcgaga tgccagatga gcttttgccg
    24901 aattttgagt ttccttctta ttctcttgaa gaaatcttag cggaagaaaa agaaatggat
    24961 cttttaggct atactccgtt acgtaacaaa atcaaacggt atttggaaag taaatttgaa
    25021 ttggcatttt cccccaacca gcttcttgtg actggtggcg gtcagcaagc aatctttttg
    25081 attttacaaa cgattctttc agctggagag gggatagctg tagagtcacc ttcttttttt
    25141 taccgattac ctcttttcaa agcttcagga acgcgtttat ttgggatacc gatggatgaa
    25201 gaagggatcg acttagaaaa actgaaagaa tcgatccata aaaataaatt gaaagcagtg
    25261 cttatcaacc cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa acgacgagaa
    25321 aagctagtag aattgtgtcg acagtatcgg cttccaatta ttgaagacga tgtatttacg
    25381 gatctttcat ttctaaacca agaagaagtc cttcctatac gaacgattga tccggataat
    25441 gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat cggttgggtg
    25501 ataggacctg agacttttat ctcgcaacta gctcaagcgc aagaaatgat ggagttttcg
    25561 atgaatatcc ttacacaaat cacagtggct aatgtattta atgaagcaat tgatttgaaa
    25621 atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc ttggggagat
    25681 tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt actatgcctg gattacttgg
    25741 gatgggaaaa aactagaccc cgaactagca gaaaaagtag tagatatggg tcttggaatt
    25801 gcaccaagtc tactttttgg gcaagatata aatggaatca gaatcaatta cagtaggctg
    25861 gatgaaaacc aaataccgtt gttttattca aagatgaaaa ttctagaaaa gtggttaaca
    25921 gaataatttc acaaagtaaa tagattaaag aatataacag cttggattta aatttcaagc
    25981 tgtttctttt ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa accattattt
    26041 gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca
    26101 acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat
    26161 gaaatataaa acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt
    26221 ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca
    26281 tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg
    26341 cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa
    26401 agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca
    26461 agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga
    26521 tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac
    26581 agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag
    26641 tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga
    26701 tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg
    26761 acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat
    26821 cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa
    26881 aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc
    26941 aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta
    27001 tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt
    27061 cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt
    27121 atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact
    27181 gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct accgtacaaa
    27241 agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac
    27301 ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga
    27361 aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt
    27421 gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc
    27481 gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta
    27541 gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac
    27601 ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc
    27661 gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga
    27721 tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta
    27781 gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca
    27841 attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa
    27901 ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca
    27961 aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg
    28021 aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat
    28081 aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg
    28141 cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt
    28201 gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact
    28261 ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt
    28321 tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca
    28381 cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat
    28441 atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc
    28501 atggcttatc aatttaaatt
//
LOCUS       DACENJ010000014        28276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.014, whole genome shotgun sequence.
ACCESSION   DACENJ010000014 DACENJ010000000
VERSION     DACENJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.014"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW748_000434"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW748_000434"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1103366.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IW748_000435"
     CDS             complement(937..1518)
                     /locus_tag="IW748_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1103367.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW748_000436"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW748_000436"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1103368.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IW748_000437"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IW748_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1103369.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IW748_000438"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IW748_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1103370.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IW748_000439"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IW748_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1103371.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IW748_000440"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IW748_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1103372.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW748_000441"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW748_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1103373.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHEYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IW748_000442"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IW748_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1103374.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IW748_000443"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IW748_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1103375.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IW748_000444"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IW748_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1103376.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IW748_000445"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IW748_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1103377.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IW748_000446"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IW748_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1103378.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IW748_000447"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IW748_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1103379.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IW748_000448"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IW748_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1103380.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IW748_000449"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IW748_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1103381.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IW748_000450"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IW748_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1103382.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW748_000451"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW748_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1103383.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IW748_000452"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IW748_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1103384.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IW748_000453"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IW748_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1103385.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IW748_000454"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IW748_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1103386.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IW748_000455"
     CDS             13972..14157
                     /locus_tag="IW748_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1103387.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IW748_000456"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IW748_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1103388.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IW748_000457"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IW748_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1103389.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IW748_000458"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IW748_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1103390.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IW748_000459"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IW748_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1103391.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IW748_000460"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IW748_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1103392.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IW748_000461"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IW748_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1103393.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IW748_000462"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IW748_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1103394.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IW748_000463"
     CDS             18232..18879
                     /locus_tag="IW748_000463"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1103395.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IW748_000464"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IW748_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1103396.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW748_000465"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW748_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1103397.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IW748_000466"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IW748_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1103398.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IW748_000467"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IW748_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1103399.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IW748_000468"
     CDS             20317..21255
                     /locus_tag="IW748_000468"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1103400.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IW748_000469"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IW748_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1103401.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IW748_000470"
     CDS             21935..22774
                     /locus_tag="IW748_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1103402.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IW748_000471"
     CDS             22750..23619
                     /locus_tag="IW748_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1103403.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IW748_000472"
     CDS             23609..24406
                     /locus_tag="IW748_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1103404.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IW748_000473"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IW748_000473"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1103405.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..>28276)
                     /locus_tag="IW748_000474"
     CDS             complement(25688..>28276)
                     /locus_tag="IW748_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1103406.1"
                     /translation="ALVYLSIGIYPGSSRSILASDAFSQFSNFHASFRNVLLGKQSIF
                     YTWNASLGLNYLSLISYYLGGIFTPLVFFFPNQMMPDALYVLTLLKVGCAGLAFWFLA
                     RTFKIPRWGHVALSVSYALISFITAHSEIIMWLDAFVYLPLVILGIHRVMDLKRPKLL
                     FISYLLLFLSSFYMGFMIGVFSFLYFIARLLTNWSEYKKTIVPYGITSLLAGGASMII
                     VLPAVLDLRSNGEALSQITTLKTEATSYLDLIMKNMIGVYDTTKYGSIPFVYAGLLPL
                     VLCLFYFASRKVPLKNKFLYGSLFAILIASFYLVPLNLFWHGMHAPNMFLFRYAYLFS
                     FLVLLLAGKGWEKIEQDDRGLLLGIILVVAALFAVAWGIKPEGSYTYVTMTSFVLTVV
                     FLGIYAFAVGFYQHKKLSFNHLSILLLLIMSAEALVNTNSMIHGILDDWNYASRSLYT
                     DPYPDLKALVNETKKDSDSFYRLENLDPVSANDSINYGYSGISLFSSIRNRNSSSYLD
                     KLGFRSRGTNLNIRYNNNTLLMDGFTGIKYNISKNESTFSKYGFRQAGQSGDYTLYQN
                     VNALPLAFTAPLSVNDVEQPVTDNLTSQTNLINTLSGLNEQYFTFYTPTLKNQLNTTI
                     TKTTTGVTYSETADNQPKELTWEVHVPANTQAYLSLFPTNFAQLESSSATITVNGTSR
                     KTQINISGQYYDLGYYPQETTVTFTASFYGTKEISFMEPKVVGLDVVAYQAAMNQIKE
                     KGVEMEATGRKASGTVNTAEDSMLVTTIPYDKGWKAKIDGKPVKVSNFKDAFLMIKVP
                     AGKHTVTFSYLPEGFTIGAILFVLCIALFILYVWYLDRNRPFYGTIDPAIEEQPRNTH
                     RRRRRK"
BASE COUNT         9175 a         5263 c         6026 g         7812 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tccgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcatgaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacg atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgc
//
LOCUS       DACENJ010000015        27750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.015, whole genome shotgun sequence.
ACCESSION   DACENJ010000015 DACENJ010000000
VERSION     DACENJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.015"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..567)
                     /locus_tag="IW748_000475"
     CDS             complement(<1..567)
                     /locus_tag="IW748_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1103407.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKL"
     gene            complement(654..1358)
                     /locus_tag="IW748_000476"
     CDS             complement(654..1358)
                     /locus_tag="IW748_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ1103408.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            1558..1824
                     /locus_tag="IW748_000477"
     CDS             1558..1824
                     /locus_tag="IW748_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1103409.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(1992..2222)
                     /locus_tag="IW748_000478"
     CDS             complement(1992..2222)
                     /locus_tag="IW748_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1103410.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            2294..2449
                     /locus_tag="IW748_000479"
     CDS             2294..2449
                     /locus_tag="IW748_000479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103411.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(2758..2982)
                     /locus_tag="IW748_000480"
     CDS             complement(2758..2982)
                     /locus_tag="IW748_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1103412.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3249..3584
                     /locus_tag="IW748_000481"
     CDS             3249..3584
                     /locus_tag="IW748_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103413.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3817..4758
                     /locus_tag="IW748_000482"
     CDS             3817..4758
                     /locus_tag="IW748_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1103414.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            4760..5650
                     /locus_tag="IW748_000483"
     CDS             4760..5650
                     /locus_tag="IW748_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1103415.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5693..6493
                     /locus_tag="IW748_000484"
     CDS             5693..6493
                     /locus_tag="IW748_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1103416.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6508..7359
                     /locus_tag="IW748_000485"
     CDS             6508..7359
                     /locus_tag="IW748_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1103417.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            7356..7715
                     /locus_tag="IW748_000486"
     CDS             7356..7715
                     /locus_tag="IW748_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1103418.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            7730..7891
                     /locus_tag="IW748_000487"
     CDS             7730..7891
                     /locus_tag="IW748_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1103419.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            7888..8214
                     /locus_tag="IW748_000488"
     CDS             7888..8214
                     /locus_tag="IW748_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103420.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            8211..9026
                     /locus_tag="IW748_000489"
     CDS             8211..9026
                     /locus_tag="IW748_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ1103421.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            9165..9476
                     /locus_tag="IW748_000490"
     CDS             9165..9476
                     /locus_tag="IW748_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103422.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            9473..9703
                     /locus_tag="IW748_000491"
     CDS             9473..9703
                     /locus_tag="IW748_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103423.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            9710..9913
                     /locus_tag="IW748_000492"
     CDS             9710..9913
                     /locus_tag="IW748_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1103424.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            9910..10209
                     /locus_tag="IW748_000493"
     CDS             9910..10209
                     /locus_tag="IW748_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103425.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            10452..10865
                     /locus_tag="IW748_000494"
     CDS             10452..10865
                     /locus_tag="IW748_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1103426.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILVALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            10878..11264
                     /locus_tag="IW748_000495"
     CDS             10878..11264
                     /locus_tag="IW748_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103427.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            11815..12039
                     /locus_tag="IW748_000496"
     CDS             11815..12039
                     /locus_tag="IW748_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103428.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            12057..12485
                     /locus_tag="IW748_000497"
     CDS             12057..12485
                     /locus_tag="IW748_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103429.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            12463..13881
                     /gene="terL"
                     /locus_tag="IW748_000498"
     CDS             12463..13881
                     /gene="terL"
                     /locus_tag="IW748_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ1103430.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            13893..15428
                     /locus_tag="IW748_000499"
     CDS             13893..15428
                     /locus_tag="IW748_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1103431.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            15508..16386
                     /locus_tag="IW748_000500"
     CDS             15508..16386
                     /locus_tag="IW748_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1103432.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            16388..16705
                     /locus_tag="IW748_000501"
     CDS             16388..16705
                     /locus_tag="IW748_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103433.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            16754..17455
                     /locus_tag="IW748_000502"
     CDS             16754..17455
                     /locus_tag="IW748_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1103434.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            17470..18360
                     /locus_tag="IW748_000503"
     CDS             17470..18360
                     /locus_tag="IW748_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ1103435.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            18383..18598
                     /locus_tag="IW748_000504"
     CDS             18383..18598
                     /locus_tag="IW748_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103436.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18610..18951
                     /locus_tag="IW748_000505"
     CDS             18610..18951
                     /locus_tag="IW748_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103437.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18951..19322
                     /locus_tag="IW748_000506"
     CDS             18951..19322
                     /locus_tag="IW748_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103438.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            19315..19710
                     /locus_tag="IW748_000507"
     CDS             19315..19710
                     /locus_tag="IW748_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1103439.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19712..20089
                     /locus_tag="IW748_000508"
     CDS             19712..20089
                     /locus_tag="IW748_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103440.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            20090..20698
                     /locus_tag="IW748_000509"
     CDS             20090..20698
                     /locus_tag="IW748_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1103441.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20698..21039
                     /locus_tag="IW748_000510"
     CDS             20698..21039
                     /locus_tag="IW748_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103442.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            21281..24070
                     /locus_tag="IW748_000511"
     CDS             21281..24070
                     /locus_tag="IW748_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1103443.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNGGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVASSMESFLKVIAANIPRAVSFLSNLFDAVQKFGSALKFMMPFLVPA
                     TTAFGALMFQLKGIPTIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIVGAVVGAI
                     AVFGYFMATNEEVRNKVISVWNDVKDSVLGVLKNIKDWGIDTWNSAKEMASNAVESVK
                     DAWSGIKEWFSNTWQGIKNGATGLFDKTVETSMNAVDSVKNAWSNTKQWFSDIWQSIK
                     DSATEKWNEIKGSIMEVAGPLITGLKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LTPVLFITSMISGGWEETKNNMIGVWNNIKESALNIWESIKNIFVSYITNIYFAALNI
                     WTGFKLTLINIWNEVVSQAKSIWINVKYFFINLWIDIKYFAIQKWIELKFGIIQTWID
                     LKYNAITTWNNIKQFFKDTWQNIKDIAYNTWISIKNSMINTWNNIKESFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            24060..24800
                     /locus_tag="IW748_000512"
     CDS             24060..24800
                     /locus_tag="IW748_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1103444.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            24797..27508
                     /locus_tag="IW748_000513"
     CDS             24797..27508
                     /locus_tag="IW748_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1103445.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            27521..>27750
                     /locus_tag="IW748_000514"
     CDS             27521..>27750
                     /locus_tag="IW748_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1103446.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKI"
BASE COUNT         9864 a         4157 c         5851 g         7878 t
ORIGIN      
        1 aagcttgttt attttatcaa aagttcttat aattttttct ctagttgaat ctacgttaga
       61 aatagtaagt ttattgagaa gtccagttat ttctgattct aatggtataa gcattttctt
      121 tttaatatta ttagcttctc tattattcaa ataagaagga atattaattg caccgtctaa
      181 aataaaatcc ttttctttaa tctttaataa agaaagtttg ttttttattt cggatgaaga
      241 agtttcatct atattcggaa taattactga tttatagata gtgaccagag atgtgtcata
      301 tttttgatta atttcatcgt ctaaccgctg gctttcagca ttaagaatat caattttgtt
      361 ttctagcgtt gacaagtttt tattcaattt acttatgttt ttctcttttt cagatataaa
      421 cgtttcccta gcatttaatt cttgagaata ttcctttacc ttactctcta aggcagaagt
      481 tttatctttt aattttttag cttcagataa tttgctacta cccataatta ttaaaactat
      541 tccaactaaa aacaatagta ctcccattca aaacgcctcg cttcttttat aaaataattg
      601 tcaaaatcat ataaaccctt accgaagttg acggctccgg catgggaatt tttttaaaat
      661 acaacttttc cgataattct aatctgttct ggcgatacaa ttctatcttc gtataagtcg
      721 tttatagaac gtaaaatgat tgtttgattt tcgtaatctt tatatatctc tttacatgta
      781 actccgtcac cacctaattc cacaatagca atttccccat cttctacgtc cggttgttct
      841 ttaacgaata cgaattgtcc attcctgata acaggttcca ttgaatcgcc tataataggt
      901 actgcgaagt cagcagcact tggaactctc tcaaattctt cctcttcgac aaccgtatca
      961 ccgtaagtaa gttctgttgg attggcagcc gaagccgaat tgtgaacagt tgggattttt
     1021 tcttttttag gaaaatctag gacattttct tgtttttgtt cttctaatcg attacttgca
     1081 aattcatagg tagcaacttt attttcgtcg ttaagctgat tatatatagg aaggatatta
     1141 tatatctgat tagatataga taaatcaata ttcatcaaat catctatatc aacattgaat
     1201 atcttagcta tctcatttaa ggtttttaat tttggtgtat attttccttt ttcccattcg
     1261 cttattgagg atgaactttt tcttcctagt ttttcggcta gttcttgttg atcaatatta
     1321 tacttttctc taagaaactt tagattttca gcgaacatag taacacctcc ttgagttaat
     1381 tgtaacaccg tttcggaaaa aatgtaacag gtttcttaga aaatatttta gtgtttttaa
     1441 agaagtattt acgcaaaata gaaatcgatt tcaggaaaac tgaaatatgc acttgacttc
     1501 ggaaaaaccg aagtatataa tttagtcaga aagttaaaga gaggaggcga ataaaatatg
     1561 gataccgcga agaaagaaaa atatacattg agacaattaa gagctttaaa gggttattca
     1621 aaagaagaac tgtcaaggaa aagcaaagtg acatcaagaa caatatttat ttacgaaaac
     1681 gatgtagata aaatgaggaa cggaaagtac gccactctag aaaaaattgc taaggcattg
     1741 ggtgttagag tttccgatat ttttttagac cctgattcgg aaaaaccgaa atcaataaca
     1801 ggtgaaacaa aacaagtcag ctaggaggtg tgaatagtgg aagagaagaa tataaaagta
     1861 ccgactcttg aagaatatac aaaaattgtt gtcgaaacag acgagcagaa cccgaaaaca
     1921 atcgcagtta ttacggcgaa tgatattgaa tcatgcgaag gttttcgagt tcgaatgacc
     1981 cctagatatg attaatgttc tttatcacgt ggaggtatag gatcgtttcc aaaactatct
     2041 tttgattgta tttgtccatt ctttttttgg acaataaact ctgattgctg ttttttagaa
     2101 atctttttac catactcaac agcatctgat ttattatcaa atagttttgt atcttcggaa
     2161 ttaccttcac cacgtacagc ccatttgcca tttctaggag atacccattg atttttaccc
     2221 ataacaattc acctcgcttt caagttaatt ctattaagag gtgaaaagaa aacaaactga
     2281 tggaggtgag attgtgagta acaagccaaa aatcactttg aatggcacac ctaatcttca
     2341 aagattagca gatgaaatgg ctagaatacg ttctagacat tggggagtta ctgtggtggc
     2401 aaccataatt gaacaaaaag aaaagcccac acagccggca agcaagtagt gggcaccata
     2461 gaacaataat attatatcac acactcgcca cttccgaaaa gaggagttgc tgctctcctt
     2521 tcaagtttgc caaagtccac gtttcttcat tttggcaact cctcttcttg gaggtggtga
     2581 cggcgagaga cgaaggaggg atgacatgaa ggaagaatca aaaataggga ttttgaatga
     2641 gaacaattac acaagattaa ttattgaaac agaagaaggg gaaaaagtag cagaagttac
     2701 attgaccgat gcaactcctg ctgacggtta tcgtattcga ttaactccta aatatgatta
     2761 accttttggt gggtgtggat cgtttccgtg actatcacgg ctttgaattt ttccatctct
     2821 gccatgaata aatagctcag atccttgatt tttagaaatc tctctagcga tttttactgc
     2881 ttctgatttt gtattagtat gtgcagtcgc tttagaattt ccagcacctt tgacattcca
     2941 accaccattg gaattaggta caacatgttg attttttccc atagttttca cctccatttc
     3001 tatagaaata ttttatcaaa tagaaatgga gaaattcaat aaacgaaagg agggatcaaa
     3061 tgacattaca agagcatcaa cacttgatgc gaaaactaaa tcaggaatac cacgaggaaa
     3121 tgaaacctag attgctaggg gaacaatggc gtgaacgcca gaagaagtgg ttagaaataa
     3181 aaaagtgact cagccgtcca aagcaatgag tcacaaacca aaaattatac ctaaggagat
     3241 tttatcacat gaataagaaa attgaaaatc ttatcgaaga attgaaacgt gaatgccaaa
     3301 agcaaggtgt ttctattatt tgtacagccc aaaaagaagg ggaacttaaa agtctcgttt
     3361 atggtgagac aacagaaatt ttactttgtc ttgctatgca agaagaacat ttagatgaaa
     3421 atctcccatt gtcagcacat attatgcgta gaattgctgt ggatgcttac gaacaagcga
     3481 aaaatgaaga agaaaatcaa ccttcaaatc atacttttgt tattaataac aaggaagatt
     3541 tagctgatgt gatgactcgt attctcaagg gggaatttca atgatgaatg aaaaaacaat
     3601 tgatctactc attattttat ttttcacgtt agtgattgta gcgaaaagtt atgtaatctt
     3661 tggcttgtta ttcgcaatta ccatgcttgt gctttgcgtg ttagcaagca gaaaagaggt
     3721 taaacatgaa cgagttagca aatcttgaca actatttaac tgatcctgat tatgctaaac
     3781 caccatatga agcgccaatt gatgaggagg atgaagatga gtaaatctac cttagaaatg
     3841 agccatcaag aatggctcga agaccgcaaa agaggtattg gtggctcaga tgttgcaacc
     3901 gtacttggat taaacaaata caaatctcct tatcaattgt ggcttgagaa aacgggtcaa
     3961 attgaattga aagatttaga aagcgaacca gcttattggg gtaatgtttt agaagaagtg
     4021 gttgctaaag agtttcagga acgtacaggc aaaaaagtac gcagaagaaa ccaagtattt
     4081 gaacatccat tacatccatt tttaagagca aatattgatc gggaagtagt gggagaaaat
     4141 gccattctgg aatgtaaaac agccaatcaa tttctcggaa aagagtggga aggtgaagag
     4201 gtaccgctta gctatctctg tcaagttcag cattacatga acgttttaaa caaagactat
     4261 tgttatatcg ctgttttgat cggtggacaa aaattcattt ggaagcggat tgaacgagat
     4321 caagagctga tcgatacaat cactgaacaa ttagtagagt tttgggaaac gaacgttctt
     4381 ggaggtatcg agcctgtaat tgacggaagt ggagcgactg ctgacttctt aaaagaaaaa
     4441 tatgcagatg tagaagaaaa tcaaacagct ctaccatcac gttttgatga acttatcgag
     4501 caaaaaagag aactcaagcg gacgaaaaaa gaaattgaat catctattcg tcaagtagac
     4561 aacgagatca tcagtgagct aggtaaacgt gaagccagta tcggtatcac acaaagaaac
     4621 attatcagct ggaaacttgt tcgtacaaga cggatgaaca cgaagaaact agcagagaaa
     4681 tatccagatg tcgcaaatga tgaagagatt tataacgtta ctgaatcaag aaggctaacc
     4741 gaaaaggaga tcaaataata tggcaacaaa tgaatcgtta aaaaatcaat tggcagaaaa
     4801 gccacagaaa caagttgcac caggacagtt agggcttaaa gctctaatga atacaccaac
     4861 aatgagaaag aaatttgaag aagtacttca tgacaatgct aatgctttta tgtcgaatgt
     4921 tatgactctt gtatctaatg acagttatct tgcagatagt gaaccgatgt ctatcatgag
     4981 tggtgcgtta actgctgcaa cattaaatct tgggctagat aagaatttag gttatgcata
     5041 tttagttcca ttcaatagta aaaacaagca aacaggaaaa tgggaaaaga aagctcaatt
     5101 catgcttggc tataaaggat atatccaatt agcccaacga tcaggtaaat acaaagcatt
     5161 aaatgtgatc gaagtttacg aaggagaact aaaaagctgg aaccgactga cagaagagtt
     5221 tgagtttgat ccaaatggta gaacgtctga tgaagtcatt ggatatgttg gctatttcga
     5281 gttactgaat ggattcaaaa aaactgtcta ttggaccaaa caagaaatcg aagctcatcg
     5341 gattgctaac aataaagatc gcgataagac aaagttaagt ggtgtgtggg catctgatta
     5401 caatgcaatg gcacgaaaaa ctgttttgag aaatcttctt tctaaatggg gaatcttgtc
     5461 catcgaaatg caagaagcca ccacatcgga tgagagagtc caaagggttc aagaagacgg
     5521 cagcattatt gctgaaacag aagttgaaga agatattcct gaaagaaaag aagcagaggt
     5581 tatatctgaa gaaaatgaag atgtacaaac tggattattt gatgcatcta atccgccgtt
     5641 aaacaaataa tgagggagct ttctcccttg cttttctaga ggagaaatac gaatgaacac
     5701 aggatatgtc aaattacatc gaaaagtgat gaattcattc gtttggacca atccttatat
     5761 gtataaatta tggagcttgt gtttgatgaa agcgagtcat gagaatcgca agatactttt
     5821 taatggaaaa gagatacaag tgaacagcgg agaattcgtc acagggcgcg atgctatcac
     5881 atctgagatg aacgaaggcg tgaaacgtga acatcaagtg aacagcggtt ctgtatggag
     5941 atggctaaaa caatttgaaa aaaacggaat gttgaacatc aaatcaacta cgaaatacag
     6001 cgttatatca ataaaaaact ggtctttgta ccaaagcagt gaacaacaaa tgaacatcaa
     6061 acgcacaaca agtgaacaac aagtgcacac aaacaagaat gaaaagaatt ataagaatga
     6121 aaagaataat aaaacatcat cgtcgcaacc acgcaaaaag cgtgtttacg acaccgactc
     6181 agtttactac attctcgcgg aggagttatt caaacagatt tgtcagaatc aggaaatcaa
     6241 aaagccgaat ctgcaaagtt gggcagataa cattcggaaa atgatcgaga tcgacaagcg
     6301 gaccgaaaac caggtacggg gaatgattga atggagtcag cacaatgtgt tttgggcatc
     6361 gaatatttta tctgctaaaa aattgcgaga aaaatacgac acaatggcag cgcaagcaaa
     6421 tcgtgattat aaaacaaaac aaactaaaac gcttgaatac gagaaattta gcacagatga
     6481 gttgcctatt tgagaggagg cgtaggcatg gaaactgttg gggaaatcat ggaaaagctg
     6541 atacagaaag tgcttgtcca acgtggcgaa tgtcctgaat gcggacagcc tttgtatgga
     6601 tggcgtacga agaaccctga tggctcagaa cgttgtaagc caacctgtat gcaatgtggg
     6661 tataaagcat tgcgagttca ggaagactta caaacagaac gaatttacaa cgagagcctg
     6721 aaagcaagag caatcaattt tttcaaaggt ggttctgttg tgcctaatca agcgttgttt
     6781 gattgcacat tgcagaatta tcaaattgtc gatcaagaaa caagacaagc ggttgaagta
     6841 accaaacgct ttgttaattc agtcttgtta ggaaatccaa gtcaccttgt tttaactgga
     6901 aaacaaggaa caggtaaaag tcacctagcg atggcagcgg cttgggaagt cttgaagcga
     6961 tcaaactacg ataaaaaaat cttatttatc gggttacagg aaatgctgga tcaaatcaaa
     7021 ttttcgtaca acaatcctga actcagaaaa acgattgagg gatcgttgat tgcagatatc
     7081 aaaacagcgg atttagtcat cattgatgat atcggttcag aactaggaaa agatgcatca
     7141 gatagtcgag cgtttggcat aaacacgcta aattcgttct tggatgcacg acagaaccta
     7201 gcaacgatta tcacaacgaa cttgcttggt gaagaactga aaaaagctta cggtacgaga
     7261 acaatatcaa gaatgtttgt caactctgat ggatttacga tggtattttc tcaaacagca
     7321 gacaagcgca taaaaccagt gaaaggtagt atcgcatgaa taaataccgt aataaaaaaa
     7381 ctgttcatcg aggtatcaag tttgattcta tcgcggaagc agagtattac gatctagcct
     7441 tgtggcaagc tgaagcgaat ggttggaaag tgaagctcca ggaaaggttt gagctgatgc
     7501 cgaagtttga actagagggc aagaagtatc gcaagatcga gtatattccc gacttcacat
     7561 tttacaaaag cggcaagctg gtcaaagtcg tagatgttaa ggggatgcag acaaaagact
     7621 ttaagatcaa ggcaaagttg ttctgtcatc aatatcaagt gccgttgata ttagccaaaa
     7681 aatatcggaa tacgttcaag gaagagcgtt tttaacgagg tggtccatca tgacaacaga
     7741 agaagtgatt caaatgcgaa ttcgaagcct tcggcgtgag attgacgatc tggaacgaac
     7801 aaaggcagtg atggtcaatg aaacggctag aaaggcaatc gatttgcaca tagagaattt
     7861 aagaagggaa atccgtagat tggaggaatg agcgtggata aggaagcggc ttggcgaaaa
     7921 ttaatgttgc tgattcaaga tgagaactgg caagaagatg aagcagtggt tgctgaagtt
     7981 cagcgtctag gaaagattgc taacggacgt atacgaaaaa agccagacaa aagaaaacag
     8041 cgcaaaggga aaatcgtcgt tgttttacac gaaggcaaaa ttttgatgca aggaacagct
     8101 agtgagctgt ctgcagaaac tggatatacg cgtgggacta ttcgaacgta cgcttggcga
     8161 aatcacaccg atcgaaaagg gcacgaatat aagtatttgg aggaagaaaa atgaacgaaa
     8221 acaaattaat caaattgggt gtagcaggag cagtaatcgt aggtattgga gttatcggag
     8281 gatttaagtt cttcgaaaaa atcgataatg gatatgtggg tgtgcgctat tcaatgaacg
     8341 gcggtatcaa agatgaagca ctgacgcaag gtgtgaaatt tgtagggatt gacaaagtga
     8401 tccaatatcc aattcgcttg caaactatcc aatcaaaaaa tatttcagta tctacaagcg
     8461 acggcaaaaa gacaacgatt gatatcaaat atgactacaa agttgattca actaaagcag
     8521 caaaaatgta caaggaattt gggaatatca cttcggaaga tatcgaaagt ggatggttaa
     8581 aatctaagct tcaaaaggtc gctcgtgaag tttatgcgaa atatagtctg cttgatgtcc
     8641 tttcaggaga ttcctctaaa gttgaagctg aggtattaac gaactttgct aaatcagttg
     8701 aatctaaagg gtttgaagtc gaagacgtaa cacttggtgt tccagatgtc gataaagaaa
     8761 cacaaaaatc aatcgatgcg atcattcgag ctggtcaaga aaatgaaaaa gcgaagctag
     8821 acgcagaaac tgcaaaaact caagctgata gtgaagctta caagaaaaca aaagctgcag
     8881 aagcagaggc agaatctaat cgcaaagtcg ccgaatcagt aacagacaat ttgattcgtt
     8941 atgaagaagc tcaagctcgc aaaaagcatg gatgggtaac agtaaatgga gcagatactg
     9001 tagttacgga tgaagcaggc aaataatatg ggattcttta tggctaaaat tctcttgttc
     9061 ttaggtttag taggagcagc atatctcgtg tatgcgctcc tttcccaaac tgatgacaaa
     9121 gaagatgaca acaacgatga aatgaaattt taggaggaga aataatggac gaactaatca
     9181 caaaagtaga gcagtgggct aaagacaagg gactggatca agcagatcca aaagcacagt
     9241 ttttgaaagt agctgaggaa ttcggggaaa tagcttcggc gatggcaaga agtaatgatg
     9301 aactatttaa agatagcgta ggagacgtta tcgtcacgct gattatcctt tccatgcaaa
     9361 aagggacaaa cgtacaagag tgtttagaaa tggcatacaa cgaaatcaaa ggacgcacag
     9421 ggaaaatggt agatggtgta ttcgtgaagt cgagtgattt ggaggatgtg aaatgaaata
     9481 cgaaatacca ctaagtgaag cgggcattca agcaattatc aatggtcggg aggttaacat
     9541 agaacttcct gatggtactg aattagtcat cagacaaagt tatttgaaag atatggcagc
     9601 tccagtatta attgatcgtt ttaacgtgac tgattctgtg gtagagaacc acttaaaaga
     9661 atttcgatca agtatagacg acactttcag attagggagt tgattgacaa tgaacaacag
     9721 acatcgcaga ataacaaaac taagaaaaca ggaactgaat gtactaaaga caaagtttga
     9781 aaaagaatat ggaatttcag cagaagaaac atataaagtg gcaagtcagt ttgttgctga
     9841 tgcaagtgat gctattcgta agtttgggat ttcgatatta aatgatgatc gtaaatggga
     9901 ggaaatgaga tgaaactaaa agacggattt tacgctagta gtcacggcat cggcggttta
     9961 atgctagata tgccgacaaa gaaccctaaa acacgtaaga aaccaaaagt caaagtcggt
    10021 gacatggttc gctgtgaagc agaagagttc atttatccgt ttcgtggata tgtagaacac
    10081 gtctataatc actcagcgat cattcgtatt gaaaacacaa tggaatgcga taagtggtta
    10141 gcgaaaagca aagagaattt agctgtagcg agattggtgg atatggaggt tataaacaat
    10201 gaagtttaaa atctttgaag aggacactcg ttataaatta gaaaaagaat taaacgattt
    10261 tgcgaaaaac aatgagattc agcatatatc tttagcaact tctaagagag gttatgcaaa
    10321 ttactatgca gctgttgtga gctatgtaag tcgagaagtg taactcggca aataaaaaaa
    10381 gccggatcgc tccgactgat tcaataaatc caacacattt attatatcac ataaaggagc
    10441 ggtttgactt gatgcaattg ttacgagagg tagatttcaa acagacaaga tgtaatgcga
    10501 gagatgtgct gaagaacttt cggcgtttgg agcggatggc aggtcgctct ttgatagata
    10561 ttaagtcgcc gattattacg gatatgccga aggcaccgaa gcacggcaat aaggcagaag
    10621 acgcgatcat tcaaatgatg gatatagaag cggagagaga cgcgattcta gtggctttga
    10681 tggctcttag tctgattagt cgtcagatac tctactacag cttctgtgtg ccagacagct
    10741 tctcaaacta cagaattagc cgtgaagtgg gttattcaga aagaagtata caacggatga
    10801 aatcggaagc tctaatagag tttgcagaag catataaaca cggaagaata attgcttata
    10861 aataggggga ccatataatg tggaaagatt atgtatcgtt gaaagagttg aaaaaagatc
    10921 ttgttttcaa aagaattgta gaatggtcag agagtgaatt aattttagaa gatggaacca
    10981 aaatggaagt tgtatgtagc gaatcagatt gctgcgcatg ggccgaaggt gaattcaaaa
    11041 atgtaaagct tgatgcagtg attacagata taaagatttt tgataaaggt aaccgtctct
    11101 ataatggtga cggacatacc tcttatgctg aagtcgttgt ttatcataat cgtaatgaga
    11161 tcgctaaagc agaatgcaca gcgaatgatg ggaatggcgg ctactattat agtgtttgtg
    11221 ctttaaaggt caaagacaaa ctttgtatag tgactgacgc ataaaaaaat ggcggttttt
    11281 tggcggaatg atggcggttt ttagccattt atcagtgata ttatggtagt gtcgaaagat
    11341 tagtgatagg tctgagacaa aataataata aaaggaacat cgttttatta ttgtttcaca
    11401 attaagcttc gatagacagc agcggaaata ttaagaataa ggatgtgaat tttaactcct
    11461 tctaaattgt tcttattatc tatcatccgt tgctgtctat tgttacatac agatcgctta
    11521 ggcggtcttt ttattttggc cgaaaaccta cattttcgat ggccgattat tggaggagga
    11581 atagcatgtt caaactatct gaaatcatta agaagtccga cgttgagaaa ttagagatgt
    11641 taaaaaagaa gttgaagaaa tagcagtctc ttcgtgaggc tgttttattt tgctcacaaa
    11701 aatagaccac tatcgggtaa tagtggtcag gaattaaatg aaaaagatgt tagggttgtt
    11761 agctaagtat aacatcataa cgcttacaaa acaatgcaag aaggataaat tattatgaga
    11821 acttactggt atgtgtcatt aaacaataaa tatccgaagc caatgaaagg acagcatagg
    11881 cgtgtagtca tgtctgttca aatgaaggcg aagtattcga ttgtagaaat gatcagagag
    11941 gcaacgccag tagagattga ttattgcaag ctagtctatt gtgggtgcgg ccgttggaaa
    12001 gaggatcatg tacaaaaaaa tatcagcaag tacatataga gaggcggtga ttaaggatgg
    12061 aaaaaagcga tacgaaagcg aacagataca aacctactgc tgcagagaaa aaactcttgg
    12121 aagtattgat aaatccagat aacttaggca aaccggtgca agagttgtgt tcactggcga
    12181 aaataggtag gaccaaatat tacgacgcca tgagcaaaga tggctttgtt gatctagtta
    12241 acgaaacaac aatggatctg atcaaaggaa aagcttctga tgtccttaat gccacttaca
    12301 agtatgcatt gactgaaaaa ggtcatcagg ataggaaaat gattctgact attgctggaa
    12361 tctatgcaga caaacaagaa ctcaaacatt ctggcggcgt cgatatccga aaacagtatg
    12421 agaaaatgtc cgatgaagaa ttagaagagt tggtgaaacg gtatgaaaaa atcaacgact
    12481 cttaatcgtg ttgagtatgc cgttgttcaa agagagctag ccattcgaaa agctaggaaa
    12541 agtttttggc tattctgcca gcttctttat ccagagtttt acaaagatga tcggttgtat
    12601 ttgaaagatt tatgtcatac gctacaagat ttttacgaag ataaaatcga caaaaaaatt
    12661 ttggttatta acatgccgcc tcgacatggg aagactttta cagcgcggct attcgtttta
    12721 tggatgtttg gacaaaatcc aaagacgaaa attattaccg gatcttataa ccaaatcctg
    12781 tcatctttat ttgcgcaaca aacacgtgat ggaatcatgg ttgaaaacga aaatgttaag
    12841 gctgaatact ttaaagatat ttttccagag gtgcaaatca aatatggaga tgctgcaaag
    12901 ggtttctggt ctttagatgg ttcagaagaa aagaattatt tagcgacttc tcctggtgga
    12961 acatccactg gtattggcgc gaattatgta attgttgatg acatcattaa aaccgcagaa
    13021 gaagcctata acgagcgtgt tttagattct cactgggaat ggtataacaa cacgttggcc
    13081 caacgaatgg aacggccacg taaacagata ttgattatga cacgttggtc aagtaatgat
    13141 ttagccggaa aaatgttgac gagacgaaaa aataatgttc atcagatctg ttataaagca
    13201 gtacaagaag acggctcgat gttgtgcaac gagattatga cccacgatga gtacttagat
    13261 gtggtacaag aaatgaatgt agatattgca gaagccaact atcagcaaga accaattgac
    13321 caaaaaggca ggctatatca gaagtttctt acttatgata cgttgcctga taacattatc
    13381 aaaatatgga actacaccga caccgccgac aagggtgccg attactttgc gtctcctgtt
    13441 tttgctgaga cgtctgatca tgatgcttat attatcgatg tcatgtatac aaaagagccg
    13501 atggaaacaa ctgaaatagc ccacgctaat atgattattc gcaatcgagt taataaggta
    13561 cggattgaag gaaacaatgg cgggcgtgga ttcagaagaa attcagaaag aattacaaaa
    13621 gagcgaggct atcgaggtgc agtatttgaa gactttcatc aatcggataa taaactgtct
    13681 agaatactgg cgaatagtgc ttgggtggaa aatcatgttt actatccaga tgattgggcg
    13741 acacgttggc cggactttta tgaggctatg gttagttatc aacgtgaagg taagaacgct
    13801 catgatgatg ctccggatgc agttacagga attgcagaaa cgattataga agcagatgac
    13861 ttcgagttta ttgcttatta ggaggtgggt ctttgtttta ttctggaatc acattgaaaa
    13921 aatacaaacg tttaagaacg aaatattcaa cgcaaatcga agaaggatta ttcgatccta
    13981 acgcttttat ggaagatatg aaaccctttt tcgatgatcg agaaagaaag tatcttgctt
    14041 atactagcga gaaaaacgag atcgacagtc gtgagaaacc aaaaacaaaa atcattaagg
    14101 tcaataacaa gattcatgcg ggaatgtatt cagtggtcgt tgatcaagca gttaaccatt
    14161 tcacaggaat accaattaaa tgggattatg atgtttcgga acagaaaagg acattcattc
    14221 aacgaatgag agacaaatta ctgggtaatg atatcagcgc cccttctacc ccagaagaat
    14281 ttgaaacact cacgagacaa ttaagtacta tgcggtttgg aatgttagac cccgatactg
    14341 caacatttca aggagcgaca gggcttgctt tcagattact ggagcctgtt gagacaaatg
    14401 atggttggga actaagagca agcaacattg agccgtggaa agctgaaaag tacgaaaacg
    14461 gcgcagtgta cattaaagag aaatacgatc catatcaaaa gaagttttat gaagagatga
    14521 aggttgtcac gccgaaatca atatttactt atgctcgata tactgattac cctggcacgg
    14581 tacaaggagc atttaaaacg ctgggtaatg ttcctaatcc tttaggcgtt atcaatctta
    14641 cggagtttaa aaacaataca aaccgttact gtgactttga agttgcagag gagattagtg
    14701 atgctattga tcgtgtttta tctgatcagc agaacgagat tgaacaattc aagttagctt
    14761 atatgctgat cactggggca gatttgtccg aggataaagc caagaagatg atggagcaac
    14821 tagggatcat caatctgaaa gaccctaatg cgaaagccgg ctacgtgaca aaggatctac
    14881 aaaaggattt caacgagtat cattttgatc ttcttaaaaa gcaattctac acggtgtgta
    14941 aagcaattga tttcaatgac gaagtcttta agtcgaatag ttctggggaa gcccgaaagt
    15001 ggcaaatcat tagtttagaa gctaagacaa ataccaaaga acagtacttt cgagaaggat
    15061 tgaaagagtg tgccgaggtt atggctgctt atttggattt tgccgagaaa gttacaattg
    15121 atccggaaaa gatcattttc accttctctc gttcgcttcc aacagattta agctacctag
    15181 ctgaggcatt gcctaagttg gcaccatacg tttctaagag gacgattcaa agtcagatcc
    15241 cgttcgtcac agatgttgat tatgaaaacg aaatgattga cgtcgaaagc agacaatctt
    15301 acccagacgg cgagtatgga ggtggcatcg ttgagtcaaa tagaaacgaa gtactggacc
    15361 aaacgtcgag aactggaaga tttagcaaga ctcaaaatgg aaaatcaaac gctaaagaaa
    15421 ctaagtagtg tctttcctga tgcgttgaaa gaaattcaag cggagttatt atcccaagct
    15481 gatttacacg atattaccta tgctgagatg atggaatttt atagtacaag tgatcagaaa
    15541 aaatatcgtg aatacgttga aaagaactac aaatcactga agatgtatga tgcaaaatac
    15601 aaagagttca tcgaagaata ctttccttct ttcgactacg ccaaagtgaa tcggctgctt
    15661 caaatgaggt cagatatttt tcgaattctt gcagagagtg caatagatgc agatgtgaat
    15721 aaatattttt cggatcggtt agaagatatc ttgcaacgtt cgtatgtatc aaacgcaaac
    15781 gtatttgccc agattttgaa cgtagatttg ccgaattact tatcaaagga agaacttgaa
    15841 cgttacctga actatccttg gtcaggtaag acattctcgc gtcgtttatg gggcaatatt
    15901 tcatctctag aacaaaagct atctaacgcc attacaacta gtgtagcaag tggcgaagga
    15961 gttctcgtgg cattacgaac aatgcgagaa gacccagaaa tatgcgatat gttcaaaagg
    16021 gaggaatcga agtacaacaa ggcgatcgaa aatcttgtga gaacagaata cgctaaattc
    16081 gctcaagatg gtattgagaa atcttatatt gaaactggcg tagacgaata caatgtctta
    16141 actgcaaaag atgaaaaggt atgttcaata tgcggtggca aagcaaagaa caacccatac
    16201 aagttgagtg aagcgatcat tggtgagaac cgtgcgcctt ttcatggccg atgcagatgt
    16261 acagatgttc ctaatatgcc gaaattagga aaagatattg acgaagagta tgagcgttta
    16321 tttggcgatt tattagatga gtttgcacat gattcttttg gaattaattt gaaacggagg
    16381 aagtagaatg aaagattttt ttgaagcagt actaacaatt aatgtaaatg ctgatattgc
    16441 agaagcctac aaaacagcta ttgagtctga gaaccatcct aatggcttga gagaccattg
    16501 gaatggcaat tatgcctacg tggttattgg cgatcaaact gttaattatc aagataatac
    16561 tccagttgat aagaataccg ttaatttaac gattcaatta ttatctcatt cattaccaaa
    16621 tttaaaagaa acagttgatt ggtatgaaaa tatgggatgt attgttgtta gaactgacta
    16681 caaagaagga aagtctagta attaggcttt tttattttgt ccgaaatgac gttaaactag
    16741 cgcaatactg ggcttggttg aatggtgggg cgcaactatt aaaactcaaa gcaatgcggg
    16801 gcgtgaaaac gaatcgtggg gcgaaaggag aatgattatg aaacacaaat cattaatgcc
    16861 aatgaatttg caatactttg ccgaaggtga cgatcaaaag ttttcttttg atgacttcaa
    16921 atcttttgtg gaatccaatg aagaagctca aaaatttatt cagtcacaat ctcaaagtgt
    16981 tgccgacaaa caattggaag cttggaaaca aaataactta gataaaatta aacaggatac
    17041 catcaaggaa tatgaggaat ctaagaaaaa caagtcacct gagcaaattc aactggaaaa
    17101 actacaagct gaatttgaag cagaaaaagc gttgcgtgtg acaagtgata ataaagcatt
    17161 tgttgcagaa caaattgctg gattagaact agatggagag ttaaaagagt ctatttctca
    17221 atttatgcta aataatcttg ttagttcgga tacagatttc actaagaatg ctgttgaagg
    17281 ttttacaagt gttttgaatg caattaatga gaaacatgca gatgcattaa aagaactaca
    17341 aatgaagtct gcatttggtg gaactcaaca atcgaataac caggttcagc agaacaatga
    17401 aacattaaca aatccagaag aacaattagg acaaattctt caacaattta actaggagag
    17461 tgaaaaatta tgaaaaaaac atctttaaat aatttagagt atttggatat ttcaccagcg
    17521 attaatgcta tgcaagtacc aaatacacct tttttaagct atttacttgg tgctggaaaa
    17581 acagagccag caaactcgac agaaattaaa tggcgtgaat atgatatcaa caacgatgat
    17641 tcttctgaaa aacttgaggg cggagaatat ccagatgctg aatcaggtcg aacttggttt
    17701 aacaactata ctgaaatttt tagaaaatca acctctgtat ctggtacatt agatgctatt
    17761 aatgtgaatg gagtcggaaa tgaattaact aatcaagtag cactacgtgg tatggaaatg
    17821 aaaattgatt tgaaccgaaa attgattact ggtgtaaaag ctgatgaaaa tggttctaaa
    17881 ggtcgtcgaa tgaatgggat tttgaacttg atcaattcag caaataaggc agaaacagcc
    17941 actgcgggtg cagtaacaag aaaagatatc gatactttat ttaaattgat gtatgaaaaa
    18001 ggttatatgg gagaaaaact atgcttgatt tctccagata tgcaggagtt aatgactgat
    18061 gagttagatg gaaaatcaac aaaaattgtt cagttcgggg aaagagtaac ttttggattg
    18121 caaattggaa atatcgtgtc taattacggt acaggtattg ctctactaga accatcattg
    18181 ccaaaaggaa caattgccgc aatcgatact aattatgtga aactacgtcc attacgtgaa
    18241 tggagagcag aagaacttgc aaaaacaacg gattctagac gtatcggtct tgtaggtgaa
    18301 tactctcttg aatacaatgc ttcaaactct ggggcaattt taaatttaaa gtctgaataa
    18361 aagggagtta gtactccctt ttttgatagg aggaattaca gtggtaaaaa aagatgaaac
    18421 taaaaaagat gaagtcgtga aatatagagt aggtaaaact aaaaattttg ttgggtttgt
    18481 tcatcctaaa actcgtagat ttatcacagc agattcaaat aacgaattta tcatttctat
    18541 agatgataaa gaagcaattg caattttgga agatgcaatt gatgttaatg aaatttagga
    18601 agtgatctga tggatgaatc gctaaaaacg gaaatcattg agtctacaaa agaagatttt
    18661 ccagatttga gtgaagaacg cataactaat ttattagaaa taattttgct agaaattgaa
    18721 tcatacaaca cttgtaaaaa tgatatttca tgggaaaagt taaaaagcgt gattagtgaa
    18781 gtgttgtata aaataataaa aaatgaatca gaaaaaacag tatcttcagt tagacgtggc
    18841 gatacaacga ttagttatgc ttcaacaaca aatgatgtta gtgagttact tctaggctat
    18901 ggtgatttga tacgaagagt tattggttgt ggaggattgg agttttttta atgaatgaag
    18961 cagatatttt agaaactact tatgaagata gttgtattat tgaaagactg acggacattg
    19021 aagattctaa tacaaatatt actattcaag attacaaaaa agtatatgat aatcctattt
    19081 cttgcgctct ttcacaaggt caaatcgatg gactagcagt catagaagat ggagagatgg
    19141 taaatgtttc aactgacaca tataaattat ttgttcatcc taagattaaa ctaaaaaaag
    19201 gagatcgaat aacaataact caaaaggcca gtggcttaat tttttctcta tttgctacta
    19261 agccttttta ctatcctagt cattgtgaag tgaatttgat aggaagtgag aaaaatgggt
    19321 aatctcaaat atgaatcaaa tgcagaaaag atcattgaga attttaaaaa tatgactgta
    19381 attgctcaaa aagaaggaat atccttcgtt aacgattcaa tgaataaagt cgttagttta
    19441 gctaaaccat taactcctgt aaaatcaggt aatttaaggc gaggatatcg tgtagtaaaa
    19501 gctagaaaac tatcaagcgg tcgaattgtt ggagcagtta taaacaatga acattatttt
    19561 aaatatgtag aagaaggtca taggactaaa aatggtggct ttgtcaaagg gaaattcatg
    19621 ttaactcgtg caacgaatct tgcaaatatg tcttatattc ctcgaagatt taaacaaatg
    19681 gcaataaaaa tcgttaagaa aggaaagtaa catgaaagat gaaatcattg ctgcaatcag
    19741 tagcaaatta aaagaaatct atcctgatgg gacaatatat cttgattcag ttatgcagtc
    19801 aactaaagat ttttatttcg ttttatcagt aatggaatct ggaactgaaa atgtaggaat
    19861 tgatattcaa aatgtttctt tcttaattga tattgcattg attgataata aacctaatag
    19921 aaatttaatt aatgaattag tctcacgttg tgggactttt tttaatacga ttacaatcga
    19981 tgagcaaata ttatttccaa aagcctattt acctgatgaa gcagatggtg ttcaacatat
    20041 tcgttttaca ttagaatttc cacaatatat tgaatggagt gaaagataaa tgggagaaaa
    20101 aagaagtaaa accggaatta tttctgtcga aaaacctact tggtttcctt tggaagatga
    20161 gacagggaat ttcccggtat atggtactgc gttgccaatg ggaacagcag taagtatcaa
    20221 accaacagct aattatgaaa caacacaaga ttatggggat tcagtggttc aagatcaatt
    20281 tacagctttt ggtggtgcag aagttgaact ggaagcaaat ggatatactc ataaagtttt
    20341 atctacaatt actggaggga aaattgttaa aggtggtgcc ttgcgttctg gagaagatat
    20401 tgcgcaagat ggtgcgtttg catatagacg taaaaaatcg aatgggaaat accgttatac
    20461 ggtcttttat aaaggacagt ttgctctaga ttcagatgaa acgtcaacga ttgaaggaag
    20521 taaagtaagt tttactcatc caacttggaa gggttcattt gtagatgtac caggacttgg
    20581 atatatgtat tcagttgatg aagatgatga aggcgtggat aaagcaatga tcggaaattg
    20641 gtttacaaaa gtagcaattc caattgaaga agcagaactt tcaggaggta cagaataatg
    20701 tcaaaatatc aaacaacgat taaattaacg aaaaaagatg aagaaggtaa atatgagcaa
    20761 gtacaattta aatctgctga atttttacca ggaacagttg tagaagatgc tgcaggagtt
    20821 atggaagaaa tgcaaacagc gactgataaa caatcagtta aaaaagcttt aagtcgtgct
    20881 tattcattta ttgcagatac tttatttgag ggacaattta ctggtgaaga ttattgcaaa
    20941 ggaattgatg ctcgtgagat tgcctcattg acaggaaaat tattgaagtc tgttactgca
    21001 ggttttgatg aaacttatac agagacgaaa aaaaagtaag tgaggcgctc aagtcacctt
    21061 catttaagta ttcgattact taccgagaat tagatataaa gacgcaatta ctcgaagcag
    21121 gttggacgtt accagaaatt gaacgtactg acttggatga gttaatgcgt ctttttgctt
    21181 ttagagatgc ggtaaaagag catgaagatg ttgagtacta tgataatttc actcaatttt
    21241 aggaggtgat actttgaata acgatgacct aattctgaag atgatactag atgaatctgg
    21301 ttttactgcc ggtatgaata atgctgtcaa aaagttgaac tcttttgatg aaacaatcga
    21361 aagaaaaagt agaaatggtg gcagttcatt aggtagtatt tggaaaatat ttgctggaag
    21421 ttttctagct agcggagtaa ctagaatcgt aggagctggc tttgatctaa tcaagggttc
    21481 cataagtgga gcggttgatc gagtagatac gatgaataac gctctacgaa atttccaaaa
    21541 tatgggattt agtaattcag aaattatgaa gaatatcgga aagaatgggc ttttatctca
    21601 aggtattcaa ggacttccta ctgctttgaa tgatgcgata agtcatgttc aacttcttgc
    21661 ttcttctaca ggtgatttaa ctcgttcaac tcaaatattt aaagctttaa atgatggaat
    21721 tcttggtttc ggtggttcaa ctgatcaagt taatgaagct gttattcaat tatctcaaag
    21781 tttctcaaac ggaaaagtag acgcacaaac ttggaactca atgatcaacg ctcaacttgg
    21841 acctactctt tctgctattg ctaaaaagat gggaattaca atgggagatc tgaaagaagg
    21901 tttatctcaa ggtaagattt ctgttgaaga attccaaaat caattaatag aaatggatac
    21961 caaaggtggc ggaggactta aatcattaag tcaaatcgct aaagattcta ctaagggaat
    22021 taaaacctct atacaaaatg caaaaacagc tgttacacgt ggtgttggtg aggtaataga
    22081 aggattaaat aaagcattag tagattcaga tttaggtgga tttaagggga ttattgataa
    22141 agttgccagt tccatggaat cttttttgaa agtaattgct gcaaatattc ctagagcagt
    22201 gtctttttta agtaatcttt ttgatgcagt tcaaaaattt ggctctgcat tgaaattcat
    22261 gatgccattt ctggttcctg caactactgc ttttggcgca ttaatgtttc aacttaaagg
    22321 aataccaaca attataaaaa gcttcaataa ttttaagaat gccataatcg gtgttggaaa
    22381 ttcactaaag attatggggg ctatagccgc cgcaaatccg tttgttttga ttgttggagc
    22441 cgttgttgga gcgattgctg tatttggcta ttttatggca accaacgaag aggttagaaa
    22501 caaagttata tccgtttgga atgatgtaaa agattccgta cttggtgtat taaagaatat
    22561 aaaagattgg ggaattgata cttggaattc tgctaaagaa atggcatcaa atgctgttga
    22621 gagtgtcaag gatgcttggt ctggaataaa ggaatggttt tcaaatactt ggcaaggcat
    22681 taaaaatgga gcaacaggtt tatttgataa aacagtagaa acatctatga atgcggttga
    22741 tagtgtaaaa aacgcatggt caaatacaaa gcaatggttt tctgatattt ggcagagcat
    22801 aaaagattca gcaactgaaa aatggaatga aattaaaggt tctatcatgg aagttgctgg
    22861 tccattgatt acaggtttaa agaacgcatt cttgcatgtc actttttatt tagagacctt
    22921 atggaataat ctagttgaga ttggtaaaaa tgtttttgag attttaaaaa atgttatact
    22981 tacgccagtt ttgtttatta cctccatgat ttctggtgga tgggaagaaa caaaaaacaa
    23041 catgattggt gtatggaata atattaaaga aagtgctcta aatatttggg agtctataaa
    23101 aaatattttt gttagttaca ttacaaatat ttattttgct gcgcttaata tttggacagg
    23161 gttcaaactt actttgataa acatttggaa tgaagtagta agtcaagcta aatcaatttg
    23221 gattaatgta aaatactttt ttattaatct ttggattgac attaagtatt ttgctattca
    23281 aaaatggatt gaattaaaat tcgggattat tcaaacttgg attgatttaa aatataatgc
    23341 tattactact tggaacaata ttaaacagtt cttcaaagat acttggcaga acattaaaga
    23401 tatagcatac aatacatgga tttctataaa gaattccatg atcaatactt ggaataacat
    23461 caaggaatct ttctggaata ttgttactgg aattgttaac tccgctgaaa atgcatggac
    23521 taatctaaaa aatggtgttt caaaagcaat taatcgggtg aaagaaatct ttgattcatt
    23581 aagggaaatc aatttattcg aaatcggtaa gaatatcatt gatggactta tcaatggtgt
    23641 agtagaaaaa tggaatgcat tgaaaaagac tattaaagga atagctggaa gtatcaaaga
    23701 ttctattaag ggtgcattag gtattcattc tccatctaga tggatgaggg atatggtagg
    23761 taaaaatatt gttcaaggta ttatcgttgg tattgataaa gaaaaaagta aattggatca
    23821 gacaatgact gatttagtga aaactccgtc ggttcaacca gttattacag ggtctaatag
    23881 tcaaccagtg gtacaagcta aacaaaatac atcttcaaat gcagtgaatg aaattcactt
    23941 gcatttaaat gtttatggag atctacctga ttcgatgatt aaacaaatcg ccaagaagat
    24001 gaaaacagaa ttgacgagac aaatgaaacg agatgctgat gcagtaggag ggacattata
    24061 tgcaacttaa aagaggacaa ttttttatca atcaacatta ttcttctgag tttaacgtat
    24121 atattcaaaa taggcctgcc tctgtttcag ctagtcgtgt aattgaattg agagaacgtg
    24181 aaggcaatga ctcaattatt atcgataaag cctattacaa aaacgttact agaaaaattg
    24241 aatgttatta caaagcacca tcgattgatt tagtgcagga atgggaagat cgaattactg
    24301 aatggttaga tatgaactct tacagtgatt ttattcttta ttacgatcaa caatatatct
    24361 atcaagctat agtaaccgaa gcgccagaat ttaaaggaac aagaaaaaca gggaacatag
    24421 ttccatttga atttacagtt agtcttcgac cattcaaaga aaattatagt ggtcgttttg
    24481 ctattcaaca gataaaagct ttcgagttat ttaatccaga gaagtatgct tcaaaaccgc
    24541 ttattaaatt gagtggttct ggagatgctt ctttttatat taataatgat aaatatgatt
    24601 tgaaactatt agatagagaa ttatatatag attctaaact agaagagtct tatcgaaagc
    24661 tagatgacaa tttagaacat caagatcatg tcactttatt tttagatttt ccatttctat
    24721 accctggaaa aaatgaaatc aaatggacga ataacataca ttcgtttgag ataataccaa
    24781 ggtggtggag aaaagtatga taccaagaat atatagtcct accgagacag atttttctac
    24841 gaatggttta gggattttga aggacaccac aaaatgtgag atatatgaag tagcaaatgg
    24901 aaaatatgaa ttagagttgg agtatccatt aggtactcga tttgatgaat attttgaaaa
    24961 tgactatcaa ataaaagcaa agtcaaatga tcaagaagag tatcatatct tttttattga
    25021 tgataaagat attgatacct ttttagatac cgtaactatt tatgcccaga gtcgtacaaa
    25081 tcggcttgga agacgagcgg tcacttttgc tggagtcgac tctaaaactg gtcgagaagc
    25141 aatggcaatt attgaaaaca atatggataa aaaatcagat atacgcttat attctgatat
    25201 tacaactgta tcaagtacaa cttttgaagc aagaaatgtt ttaaattgta ttgctggtga
    25261 acaaggatct ttacttcagt attggggcgg agaaattaaa cgtgagcctt ttaagctatc
    25321 tttattaaag cgaagaggtc gtgataatat tggcacaatc cgatatggaa aagatttatc
    25381 tgggttaaaa gttaagttag attggacagg cgtgaaaaca agaattattc cgtatgctga
    25441 tcctcaaagt gatgcaggta caactagccg aatttatggc tcgccagtag atagcgaata
    25501 tattaataat tatcctgatg tatatacaga acacgttcaa ttcacagaag aacagggagt
    25561 taaagacgtt aatagcttaa ataaaatagc taagaattat ttcaaaacaa ttaatccagg
    25621 ctgtgataaa ccgaagattt ctattaccgt tgagttcgat aaattaacgg acaccgaaga
    25681 aggaaaagaa ttcgctaaga taagaaatta tgggttattt gatacgttta aaatatatca
    25741 tcgaaaatat aaactatatt ttgaatcgaa agttagcggt gttcaatatg attcattatc
    25801 tgaaaaggtt ttgaaattgg aagctgggga tgctcaagtt gctttttatc aacaacaagc
    25861 tgttactatt caagataaat taaaagatta tgctacgaat aattatatga gtagttttaa
    25921 tgattatgtg tcctctatga ttgcaggtca aggaaatgca ggcggttatg tagttttatg
    25981 gccaaaagaa aaaccttcaa atatttttat aatggatagc cctgatttaa acaaggcaaa
    26041 agaagtatta agaatgaaca aaaatggaat tgccttttct aaaaatggtt ggaatggtcc
    26101 ttttaattcc gcttggacat tggatagtat attcaatgct aattttattc aaacaggatt
    26161 aataaaggca gatatctttc aaaactcttt taataaaact ggagatgtat tgaaattagt
    26221 taatggacta cttcaaattt ggaataacaa aaagaaaata atggagttaa ccaaaaaagg
    26281 aatggagttt tggaattcta atagttcaat tggaacgatt ggaacaactg attctgctgg
    26341 taatcctttt cctggagctt ctactcccac acctattgaa gataattctt tagttattcg
    26401 tacaaatgga gacggcaaat atattttgat ttctcctaaa gttggtaaag gattagtttt
    26461 attaggaaat ggtaaagcaa tttattttgg agacttagat gtacaaggca aactcacagt
    26521 taacggaaaa gaaatcacag ggaacaacag tggaggaagt gatcctggaa ctatccctcc
    26581 tcaattgacg acggaagctg aaaaaagagc atggaaaatt tggacaatgc tgaaagctcg
    26641 tggctattct gaatatgcag ctgcaggtat tctaggaaat attcaggggg aggttggagc
    26701 aagtatgaac cctgatacag aacaacttgg tggtccagct tatgggattg ttcaatggga
    26761 tggttctgct tatccgttag tcggatcacc aacttggaac ggacgagagt atgttcagcg
    26821 tttgatgaac accgcaggga ttcaagaaga ttatcgaagt attgaagccc aagtaaaatt
    26881 attagattgg tgtatgttca acggtcaatg gctcggaaaa gtaaatccaa ccacagtatc
    26941 aggatttaaa tcgatcaatg atgccaaaag tgcagcgtat gcttttgaaa tgaacttcga
    27001 acgcccagct tctgcacatc cagaacgcca aaattatgcc caatcttggt ataacaaatt
    27061 acatggatta actagtccag aacctggagg gaatttcatt tgtccaattc aaaaaccagt
    27121 gacagttact tcagaatgtg gatggagaac tagtccaata aatggcggcc aagaatttca
    27181 taatggaatt gatcttgtaa atggaaatcc taatacacct gtttttgcag cattagatgg
    27241 ggaagttgtt caggctggtg ctaattatta tgactggtat ggtaattacg tggttattaa
    27301 acataataat gggaagtgga caggttatgc tcatttgtct cgtattgatg tttctgttgg
    27361 acaaaaagtc cagaaaggtg ctcaaatagg cttgatggga acaactggtc catctacagg
    27421 agaacatcta cattttcaaa ttatgaaaaa ttattggcca cagccagttg ttgattttga
    27481 gaatccaaga aattatatcc aattttaagg tggtgattct atgagtaaat ggaatgtcgt
    27541 tttaagtaca acagaaccat ataattatgt ggggatgatt caagttcgac aaggcaataa
    27601 gaatacagag gttatggaag cgactatagt tgaaaatggt cttccctacg atttatcaga
    27661 atgtaaggta tattttgaat cagttgtagg tgggaaatat ccagtccaat tagaaacaaa
    27721 aattgtggat gctaaaaaag ggaaaattaa
//
LOCUS       DACENJ010000016        27465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.016, whole genome shotgun sequence.
ACCESSION   DACENJ010000016 DACENJ010000000
VERSION     DACENJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.016"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..608
                     /locus_tag="IW748_000515"
     CDS             <1..608
                     /locus_tag="IW748_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1103447.1"
                     /translation="FLAACQSNTNQGTSGNTSSSSEQRSSQVSDSSTKASTSSSSQTK
                     SSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYFYKIEGVDDQKEYEA
                     RIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAAKIAVDKVGSGTATD
                     WDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            692..1636
                     /locus_tag="IW748_000516"
     CDS             692..1636
                     /locus_tag="IW748_000516"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1103448.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1771..3222
                     /locus_tag="IW748_000517"
     CDS             1771..3222
                     /locus_tag="IW748_000517"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1103449.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3243..4079
                     /locus_tag="IW748_000518"
     CDS             3243..4079
                     /locus_tag="IW748_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1103450.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4079..4738
                     /locus_tag="IW748_000519"
     CDS             4079..4738
                     /locus_tag="IW748_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1103451.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4864..5553
                     /locus_tag="IW748_000520"
     CDS             4864..5553
                     /locus_tag="IW748_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103452.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5559..6515
                     /locus_tag="IW748_000521"
     CDS             5559..6515
                     /locus_tag="IW748_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1103453.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6559..7071
                     /locus_tag="IW748_000522"
     CDS             6559..7071
                     /locus_tag="IW748_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1103454.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7327..7875)
                     /locus_tag="IW748_000523"
     CDS             complement(7327..7875)
                     /locus_tag="IW748_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1103455.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(7964..9145)
                     /locus_tag="IW748_000524"
     CDS             complement(7964..9145)
                     /locus_tag="IW748_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1103456.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9381..10889)
                     /locus_tag="IW748_000525"
     CDS             complement(9381..10889)
                     /locus_tag="IW748_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1103457.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11048..11239
                     /locus_tag="IW748_000526"
     CDS             11048..11239
                     /locus_tag="IW748_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103458.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11442..12359
                     /locus_tag="IW748_000527"
     CDS             11442..12359
                     /locus_tag="IW748_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1103459.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12352..13425
                     /locus_tag="IW748_000528"
     CDS             12352..13425
                     /locus_tag="IW748_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103460.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13573..14091
                     /locus_tag="IW748_000529"
     CDS             13573..14091
                     /locus_tag="IW748_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103461.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14072..14464
                     /locus_tag="IW748_000530"
     CDS             14072..14464
                     /locus_tag="IW748_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1103462.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14540..15475
                     /locus_tag="IW748_000531"
     CDS             14540..15475
                     /locus_tag="IW748_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1103463.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15608..16387
                     /locus_tag="IW748_000532"
     CDS             15608..16387
                     /locus_tag="IW748_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1103464.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16380..16697
                     /locus_tag="IW748_000533"
     CDS             16380..16697
                     /locus_tag="IW748_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1103465.1"
                     /translation="MTKLYLLLLVVCLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            16941..18551
                     /locus_tag="IW748_000534"
     CDS             16941..18551
                     /locus_tag="IW748_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1103466.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18708..19556
                     /locus_tag="IW748_000535"
     CDS             18708..19556
                     /locus_tag="IW748_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1103467.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19787..20266
                     /locus_tag="IW748_000536"
     CDS             19787..20266
                     /locus_tag="IW748_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103468.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20299..22527
                     /locus_tag="IW748_000537"
     CDS             20299..22527
                     /locus_tag="IW748_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1103469.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22656..24299
                     /locus_tag="IW748_000538"
     CDS             22656..24299
                     /locus_tag="IW748_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1103470.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKINK"
     gene            complement(24372..24917)
                     /locus_tag="IW748_000539"
     CDS             complement(24372..24917)
                     /locus_tag="IW748_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1103471.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(24940..25425)
                     /locus_tag="IW748_000540"
     CDS             complement(24940..25425)
                     /locus_tag="IW748_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1103472.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25415..26227)
                     /locus_tag="IW748_000541"
     CDS             complement(25415..26227)
                     /locus_tag="IW748_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1103473.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26366..>27465)
                     /locus_tag="IW748_000542"
     CDS             complement(26366..>27465)
                     /locus_tag="IW748_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1103474.1"
                     /translation="LMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAHSMGW
                     RTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKVVHML
                     EAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTSMAQD
                     AVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLPENTD
                     GHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLIETIN
                     STLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELPRLNV
                     PIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         8988 a         5010 c         5505 g         7962 t
ORIGIN      
        1 tgtttttagc tgcttgtcaa tcaaatacca atcaaggcac atctgggaat acgtcttcca
       61 gtagcgaaca gcgctcttca caggtatcgg actcctcaac aaaagcaagc acatcttcgt
      121 ctagccagac aaaaagttct agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag
      181 ctcgtgcgat cgaattgttc caagataaat acccagatac tgcaattact agtttggaat
      241 tagatagtga ttggggatct tacttctaca agatagaagg tgtcgatgat caaaaagaat
      301 acgaagcaag aattgatgct caaaatgaaa aaatggagac agagaatcca gaaactctag
      361 atcgagatga acaaaatggt caaaagaaaa aagaagatgg tctggatgtt gataaattga
      421 tcacgattga ggaagcagca aaaatcgccg tcgacaaagt aggaagcggt acggcaacag
      481 attgggattt ggataaagat ttaggtacta cttattggga agtcaaagta aaagacggca
      541 atcaaagtac atcagtcaag atcaatagcc aaacaggcga agtactgtca actgaacaag
      601 atgattaaga atttcttcgg aaaacaagag ttgatttttc ttttagtcat gatacactag
      661 acaaaataac ttgtttgaag gagcgattta cttgagttta gaaacgacct ttgccaatca
      721 tatctttgcc aatcctttga tgaatgcatc tggtgtccac tgcatgacta cccaggaatt
      781 agatgaactg gctcattcag aagcaggagc atttatcact aagagctgta cgatcaatga
      841 acgaaaagga aatcccgaac cacgctattt tgatgttcct ttagggagta tcaattctat
      901 gggacttcca aacttaggtt tttcttatta tctagagtat gctcttgcgt atgaaaaagt
      961 gcaggaaaac caaaatcaac cgttattttt ttccattgcc gggatgagtg tccaggaaaa
     1021 cttggaaatg cttgagaaaa tagaaaaaag tgggtttaat gggatcactg aattaaattt
     1081 atcttgtccc aatgtgcctg gaaaaccaca actagcttac gattttgagg ccacttatga
     1141 aacactgaaa gaagtattca gcatcttttc taagccgcta ggaatcaaat tgcctcctta
     1201 ttttgatttt gctcattttg atcagatggc tgatatcttg aatcagtttc cattgaccta
     1261 tgtcaatgct atcaacagtg tgggcaatgg tttgtatatc gatacagaac aagaagcagt
     1321 agtcatcaaa ccaaaagaag ggttcggcgg cattggcgga gagtatatca agccgactgc
     1381 tttggcgaat gttcgcgctt tttatacacg tttgaaacca gaaatccaaa tcattggtac
     1441 aggtgggatt cggacaggac aagatgcatt tgaacatctg ctatgtggcg caagcatgtt
     1501 acagattggt accgagcttc ataaagaagg gccggaaatt ttttctagaa tcataaaaga
     1561 attaacgcag attatgtcag aaaaaggata tacgtcaatt gacgaattta aaggcaaatt
     1621 acgcaccatt tcatagcaaa gagacgaagg tcatgattga tcttcgtctt tttttataaa
     1681 aaacactcgt ctgttcattc ctgtgcaagc gtactcattg tatgttagac gaacttgtgg
     1741 gaaaataaca gaggcaaaaa ggaggataat atgaatgatg caacagaatt agcaaaagag
     1801 cttaaagaaa aagaatttag ttttcaagaa ctagttacgg atatacagaa aaaaataaag
     1861 aagcaaaatc atgaattgaa tgcgtttgtc gcgcttgaac cattcgagag taattcccac
     1921 gagctattga ttccccagtc agaaaaaggg ccattggctg gtatcccatt tcctttaaaa
     1981 atgctcggtc aagaaaaaaa gggatggcta gctacttcag gatctcgttt atttgaaaca
     2041 caccgagctt ctcatacatc taattatgtg cggcaagcag aagcgatagg tctagttcct
     2101 tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc agtcatctat
     2161 ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag tggaggagct
     2221 gctgctgcag tttcatcagg aatcgttcct ttagctggtg ctagtgatgg cggtggttct
     2281 attagaatac ccgcttcgtt ttgcggattg attgggctga aaccttcgcg agggacgatg
     2341 ccagttggtc catatgcttg gagaggatgg caaggagctg ctattgattt cggcttgaca
     2401 gtttctatga gagacacgga ggctttattt tatgggatga gaagtatcca ttccggtgcg
     2461 ccatatcaag tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg gttgaagata
     2521 gctttttgtg ttgattcacc gatccactca cagattagtg aagaggcagt acttgctgta
     2581 aaaaaagctg tagatttcct tggaaaagaa gggcatgatt tgacagagat tccttaccca
     2641 ctagatggtc gtaaactgat cacgtcttat tatcagatga atggcgcaga aacagcagca
     2701 atgatccgct cgattgaaac aggcatccag cgagctgtca gaaaagaaga acttgaaccc
     2761 atttcttgga ctttgttcca atatggtcag aagctctctg ccgctactta catcgattct
     2821 ctgcatgtat gggatgaagc agcaattgtc atggaaaacc tacatcaaga atacgatcta
     2881 ttcctttcac caacgactgc atttaccgcc cctaaaatca gcgaagattt gcaaagtgat
     2941 gcgataagag cacagatgga acacatcagt gattgttctg aagtagaagg tttagacgtt
     3001 gtttcttcca tgtttgagga gagcttgaag atcacacctt atacacagtt ggctaatctg
     3061 actggacaac cagcaatcag cttgcctact catttgacaa aagaaaacat gcctctagga
     3121 atccaattta tggctgccag aggaagagaa gatttgctat ttcaaatcgg ctatctattt
     3181 gaagcaaatc atcagttcca tttaccaaat ccatggaaat agcagaaaca aggagaagaa
     3241 gaatgaagaa aaaaagattg tttgctctaa taactagtgc cttttttatt ttaagcgctt
     3301 gcggtacagc tgccgctaat caggaagata acagctggag taatgtagaa aaaaagaaag
     3361 aattagtagt agcgacttct ggcacactgt ttcccgcatc ttattataat gatcaaaatg
     3421 aattagttgg ttatgatgta gatttggcaa aagaggtggc taaacggtta aatgtcaaaa
     3481 tctcctttaa agaatacaac gtggatggac aaatcacttc tgtttcaaaa agagaatcag
     3541 attttgctgc gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc tccctttcag
     3601 agactttcaa atattcattt gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc
     3661 actcacttga agatcttaaa gggaaaaaag cagctggcga acccaatact agctatatgc
     3721 gtttagcgga aaaacacgga gctaaattag tcacatatga caatgccacg aatgatcagt
     3781 atttaagcga tgtagcaaat ggtcgaacag atgttattct aaatgattat tatctgcaga
     3841 aaatggccgt atctgcttta ccagatatcc ctgtaaaaat actggagaac gtttatttca
     3901 atgaaaacga aagtggtttt ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg
     3961 atggcgtatt aaaagagatg aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt
     4021 ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat
     4081 gtactttcct ctaattgatt tttccctcat gtggacttca cttcctttcg tactgcaagg
     4141 cttggtatac acattaggga ttggcttcgt gtcgtttctt ttaggaaatc tggtagggtt
     4201 gcttttgaca gttttaggtc ttttggattg gctgcctctg aatgtattta ttcgttttta
     4261 tttatcgttc tttagaggca ttcccgcact agtcctgctg tttctgttat attttgggtt
     4321 accttatcaa ttaagtgcct taacggcttc agtcatctgc tttacgatta ccagcagtgc
     4381 atttattggt gaaatctatc gaggatcact agcaggcgtt tcttcaggtc agtgggacgc
     4441 tggtttagct tctggttttt ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc
     4501 ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg gatctagtga aaggtacttc
     4561 gttagcagca acaatcacag tgccagaaat atttcaaaaa gcaaagatca taggcggtcg
     4621 gacattcgat tatttttctc tctatatttt agttgcactg atttattggg tcatttgtat
     4681 cgggatcgaa aaagtgcaac agtatttaga aaaacgttct atgcatcagt caatctgaaa
     4741 aatagaaatg gtgctagcgt ggctggcacc atttctattt tttgtttctt gtatattctg
     4801 cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa
     4861 aacatgaaga ccccgctaac catcgaaata gaatcctcgt tataccaata ttgcatcgag
     4921 caaggcggct tggttgtaga agaagtcacg atgcctgatg accaaggaat tgtagatacg
     4981 cttagctgct tgacaaaatt tgatggaagc cgagaatggc gatgctatga gctgaaagtg
     5041 acaaaagcag attttcgctc tacagccaaa ctgtcatttg ttggacatta taattatttt
     5101 gttttaccaa gtaaactata tgaagaagta aaaactgaaa tccctacagg aataggtgtt
     5161 ttagtgtatc gtccgtacac ggttgaagag gagatgcctg cctctggtac atttctgatt
     5221 gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag aagctttgac aaaccgcttc
     5281 atggcttctt tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat
     5341 tttccaacag atcaattgta caaagaattg aaaagacgtt cagaaaatca agatccattt
     5401 tctaacgaac gatattatga acggttttta gaggaaatag ctagtcaggc aatcaatgag
     5461 ttgcaagaag aagttatcgc gcttcagcat gattatgaat atttgaggca acaacagcag
     5521 atccgtcggt tgcctacaga gccattggag tgaaaaatat gtactatcct tatttaagag
     5581 gaaaacaatt tgacctatta gcattaaaag aagcactgtc gcgaggattg ctctccaata
     5641 aaatccaacc agtgatcgaa ccagtacgtg attccgctac gcttaaaaat gtgatcgaat
     5701 tatttcaaaa aaagcaccat cgaatcgctg tgatccaaaa tccccaagta ggacaattca
     5761 aattattcga tcagcatgtg cacagttgga cagtcaaaga aaatagctct gtcgtccctg
     5821 cacagatctt aacgccagaa aatctcagtg aagtactgga ttcacctcca gcgtttcttg
     5881 ttttcgatgg acagcactac ccaaaagata cagaggtgtg gaaacagttg gcaggagttg
     5941 attcgaaatt tttgatacca gatactagtc gctttcgcat ctggctgccc gaaaacaaaa
     6001 ttgtgatccg tgactctttt caaacgagaa agcatgttga aagttatgca gataaaacag
     6061 atgacttttt tagtgatgac tatctttttt tccacgcaga cggatatatt ggattttctg
     6121 atttcacgat cgaaggtagc cgttatttcg ataaagggtt ccctagccga gcgcttgcta
     6181 tccatctaac gtatattgat gcttacggca atatacgcgt gaaacatttt gtctctgaca
     6241 gtaacgactc tgctaaagac caagcattga agtttcttga agcaggagat aaaatgaaaa
     6301 aatggatcat gcgtcaccat catcaactct tgattacttc aggaatgatt gaattattgg
     6361 ctctttacca gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc
     6421 atcatttgga acttattagt cacctgctag atcatccaaa agattggctt aaatattatt
     6481 caaaagttca ggaactatat gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa
     6541 tagaaaagag gaaactggat gtatattcgg caaacaagga aagaagaagt agaagaagtg
     6601 gtagaaatca ttaatgccgc agcggatttc attgcttcgc aaggctctcc tcaatggcaa
     6661 aacgggcaga aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa agaaagctat
     6721 gtattaatag tggaagatca aattgctgga acagctgcat tagtctcaag cgtggatccc
     6781 gtttacacaa aaatagatgg tagttggaaa gaatcaactg gtccttattt atcgattcat
     6841 cgtgtcgctt tagcagaaag tactcgagga aaaaaactag caaaaccgtt cttagaacat
     6901 ttgttgacga ttgcagccag caaaaatatc caagatatcc gtatcgatac ttatccagct
     6961 aatgaaccga tggaaaagac catctattct gctggattca cttatcaagg gatgattgaa
     7021 tttccttttg aacacggcga aagaaaagca tatcaaataa tcatcaattg actataaaat
     7081 catctgagca aagataaaga cattaacact actgaaaaaa acaatcatat gatagccaag
     7141 aggttgtaac agaagtggcc agcttcaaaa aacaagaaag aatttccgaa aatgcttctc
     7201 aaatttttgt ggaattttag cgtattttac gaaatagctg ctttctgaaa ctcccttcat
     7261 cgagaggtcg tgacatagct tttgtcacga cctctcggct tatcctacga gtattattcg
     7321 atacatttat atcaataatc ctttttttat tttatctgca gcttcttgga acgctccatc
     7381 gtttgacacg actaaaagtg aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc
     7441 tacataaacc gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg
     7501 cgattgcaat gtttctttag agatcgtaat aaagatagga cggactaccg tcccgaaata
     7561 ctcatataaa tgccaaaaac catttgctgt caaaacggcg taacaatttc ctactgtcag
     7621 tttcttttta atttctgctt tactggaacc atatttatgt tgagcatatt ggtcccactc
     7681 tgcaaactca tttcccttga tcatctgatt gaatgtattg tcagaaacaa aataataatc
     7741 ctcttgatct ttttccccag gtcttggtga tcgattcgtg taagtaatga tcttttgttc
     7801 cggctgaaaa atagcttctg cgagtgaagt ttttccagaa ccagaaggac cgataaatac
     7861 atagcaataa ttcattgtca tttctccttt gatgattgaa caagtgaaaa agctttggaa
     7921 aatatttcca aagcttttaa aagtaacttt gcttttttgt atattatacc agcaagccag
     7981 tcacaatagc acttaaaaag ctgactaaag tcgctgttag caaaattttc aatccgtgtt
     8041 ttgcgacaag atttcctttt tcttcattta atcccttcat cgcaccagaa atgatcccga
     8101 tagaagagaa gttggcaaat gaaacgagga agacagatat aattgctgtt gtacgctcag
     8161 taaaatggaa attaccattt gctaattctg tcatagcaac aaattcattt gtaaccagtt
     8221 tagtcgccat gatactaccg gcatccactg cttctttcca tgggattccg ctgatgaagg
     8281 caagaggggc aaagatgtaa cctaggatct cttggaaagt gatgccgaat atcccgtgga
     8341 agatagcatt gatcatggca atcaaagcaa caaaaccgat aagcatagca gcaaccgtga
     8401 tcgccacatg gaaaccatca agaatgtatt ctcccaacat ttggaaaaat gtctgttctt
     8461 tgttttcttc aatgatcagc tcatcttctt tttcttctaa tacataggga tttacaattg
     8521 aagccagaat ataaccgcca aataagttca aaacaagggc agtgatcaca tatttagaat
     8581 cgatcagtgc catataagat ccgacgatcg acatagaaac tgtagacata gccgatacac
     8641 tcatggtgaa caaacgtttc tcagatagga agggtaattc cttttttatg gaaataaaaa
     8701 cttccgattg tcctaaaata gcagaagcta ctccattata agattccaat ttgcccatcc
     8761 cgttgatttt acttaatcct agaccaacag ctttcataaa taatggaaga atcttgatat
     8821 agcgtagaat accgataata gcagaaataa atacaattgg catcaatacg gaaagaaaga
     8881 aaggtgcact tcctttattt gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa
     8941 aagcaagaag atgatcaaac atttttgcca atacagaaac aaccgctgta ccaaaagttg
     9001 tacgtaataa tacgaaacca agaacaagct gtaaaacgaa catcacaatt agtggtttca
     9061 atttgattgc ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg acaaataaac
     9121 caataatacc taataaataa ctcaaataat cactcctatt ccaaattccg ttttatttta
     9181 cctgttttgc aaataaaata ctaatcttta gtgaaatttt cattactttt ttagagtgaa
     9241 acgcattcat tttccattag aaaataaagc gaacaaaata aaaggtttaa aaagtgtaac
     9301 gtcttagtaa agtctataaa aaaacgaaac tatcgattaa gctaatagtt tcgttttcaa
     9361 catttataag taaacttttt ctattggtaa tatagatttt cggaagggta gacaggtgcg
     9421 caggtaatat cgattcctag ttttttcaat gtttgttcat catttttatt gaccatcaca
     9481 gtagagtggg cttgtgctcc ctcaagttca gctagtttat cataagcaac ttgagcgatt
     9541 ggattcgtta ctgcactgat tgataaagcg atcaagactt cattgatgct tagtgcatcc
     9601 aaagaggaat gaagtgtctt tgatttcaat ccttgtattg tttcaagaac taatggtgac
     9661 aataaaggaa tgtcatctgc gatatgggct aaatgtttta ctgcatttaa gataactgcc
     9721 gctgacgcat ccatcaatga acttgtacgc ccggtaatga tgacttcatc tggtaattcg
     9781 attgcaatca cagcagaagg atcttttgta ggactttgcg cttgaatctt atctgcatag
     9841 tcatgggcag ggactaccac gtttcggtct tcaggacgta aaccgacttc ttccataatc
     9901 agtttgattc gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt
     9961 tggaaataac gacggatgat ttcttgttta gaggcttctt ggacaacggt atcatccacg
    10021 atcccaaatc ctacacgatt cacacccatg tcagtaggag attgataaac agattcttta
    10081 ccagtaattt tttcaataat acgtttgatt acaggaaatg tctcaacatc ccgattatag
    10141 ttcacagcta ctttattgta ggcatcaaag tggaaggaat cgatcatatt gacatctttt
    10201 agatcgactg tggctgcttc ataagcaatg ttcagcggat gtttcaacgg aacattccat
    10261 actgggaaag tttcgaactt ggagtaacca gctgcatgtc ctttttggct ttcatgatac
    10321 agttggttca aggaagtagc gagctttcca cttcctgggc caggagctgt cactacaacg
    10381 attggtttag ttgtttcaat atactcattt tttccaaaac catcttctga gacgattttt
    10441 tctacgttga ctggatagtc ttcgatttct gagtgtttat agaccttgat gtttcgtctt
    10501 tctagtttat tgataaagac tttcgtggca ggctgtccac tatagcgagt gataacgaca
    10561 ctgttgatta ataagccata accacgtaat tcatcgatta accgcaagat atccatatca
    10621 taagtaatcc cataatcacc acggatctta ttacgttcaa tatctccagc atagacgcaa
    10681 atcagtattt cagcttgatc tttcaatttt tgcagcaatt tgattttcgc atcttcttca
    10741 aatcctggaa gtacacgttt cgcatgcttg tcgtcaacta gcttcccacc aaattctaaa
    10801 tataatttgt catagtggtt cactcgttcc aaaatatacg ctgactgctc ttcaatatat
    10861 ttttctgaat caaaaccaat ttttttcact aggttccctc cttaagtact aaagcatttt
    10921 ttcatttttt tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa
    10981 tggtttaaac caacttgtct agatagttat tcattggtat gatagttttt gataaggaag
    11041 gtgctacatg aaaaaaagac agaaaaagaa aaacgcatat aaacactata tccgttcgat
    11101 ttttaccggt tatgaaagaa tgcttgaaga tcccgaattg gaacaattga cgtttactta
    11161 tctaaacgaa gaaacgcagc taactcgtga tgagcatcag cgcattcatt tcacaacaag
    11221 agatctgcct agtaaataaa aaataagata aaggtcatcg gattcgattc tgtcctatca
    11281 gatggaaaga ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta
    11341 tggagcattg cccgctcttt tttataacaa ttttttacag aaaatggtac tattgaagta
    11401 ttaataagtg aaaaacagat gagtaagagg aggcaagtag tatgaaagag ccagatacaa
    11461 atgcaaatta tgagccagac gaactagagg agacgaatgc ggatcaggat acaggacaag
    11521 aatcgtcttc tttttacgat atcaaagaaa aagcaagcat gaatctttcc caattattag
    11581 aagacttaca ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag atctaccgtt
    11641 tatacaatgg ccgattgaat gaagaattaa aaagaagctc aaatgaaaat cacgagatcg
    11701 atcaattgct gatgcgtaag atccatgatc gttttttaca ggaatttcct tttatggaac
    11761 aagtagaaaa catctcgcca acgatgagct attacaaaat cggcacttat taccatgatc
    11821 ggcccacggt cggtattgat gcaagtctgc cagaaatttt tgttttgcct aagatcgatg
    11881 aagagtggga gaaatattcc caaccggagt caacggacta tgacaaaaaa atcaatgagc
    11941 tagacgcaaa ggtgatcact gctcagacag aaattgaacg tttagatgaa caaataaaag
    12001 aaatcaatcg acaaatgact gatttgaacg acaataaagg attcttgaat cgtaaaaaaa
    12061 tcgaggaaga aattcaagaa ttagagaaga agaagcaagt actatctaat gaaaaattag
    12121 gctggcttcc ttatattgag acgcctgaaa caatccagca gcaaaaagaa gcattgaagc
    12181 aagaagcaag agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca
    12241 gacgctattt cggtagcaaa gagggattcg gtcaagcaat ccatgaattc ttgatgaatt
    12301 atcttggcaa tgagaatcct aataatcaat ctaatgaagg aggttccgag tatgaatgaa
    12361 cacgatgaag cagaatggac aaaaaatgaa gaacaaacag aagatcatga agaaacgaac
    12421 acttcattgg aagaacaaac aagtaatcct ctggatgaca cagaagaaat ccctatgcta
    12481 gaggaaacaa aaatacacga aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa
    12541 aacacccatg cttacgaaat aactgaaaat cccgggtatt tagaaggaaa agagcaagat
    12601 catcaagata tcgtcagttt gattgaaaca gcggaagcag aattagcgga tttacgtatg
    12661 agaaaagatt tgatcgaatc agattttcca caattattag aatcatatga tcgttttata
    12721 ttgaatgaag aaccaatctc cacgatcgga aagacaagcc tgatcgaata cttcacgtat
    12781 gaattactga aaccgttgaa tgatattggt ctggaacggt caaattcagc ttatgacaca
    12841 tggaaaacaa gacatttttc gatcaattat acgttgaatg gacaaaacga attagtcttt
    12901 tcctttgtat taccgaccat cgatcaacga taccaagtgg aatcgatgag tctcctaaac
    12961 gtatcaccag agacaatgga aatcaatgtc caagatgacc gggtgctttc tttgatccga
    13021 cactggtcag tggatcgagt attctctgca gggcagatca cgatattcaa tcacaaattg
    13081 aatcaattat tagcccatgc aagaacactt ggttttaccg ttaatcagac attactagac
    13141 aatacgaagc cacttcgttt gaatcttcag agcgaatttg aattgacgaa tgaagtattg
    13201 gatgatattt ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa
    13261 actacttatc aagtacgatt agataaagga cagtctttaa cgataacaac aaacgaaaaa
    13321 gatcaaacag tcttgtcgat ctcatctgga gactacccac gatctgtcat tgatttcttt
    13381 atcaattacg aattcttggt cccattaatg gtgagaaagt cctgattgga taagttggat
    13441 taccagatgt cattcagata attctgttcc aaaggacttg cggcagttat ccagaataat
    13501 tgtcacaagt cttttctgta tgtactaaat ggacgtgaat aacttgctga tgaaggaggg
    13561 atttcattag gaatgtccac aaataaaata gcacagctta gcttattgtc tgctgcttgt
    13621 gtagcaggta ggatcgcttt tcaatttatt ccgaatttcc agccaatgac tgctattttt
    13681 ttatttatag tactttactt aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt
    13741 gcgatcagca gtttttattt tggtgttgga ccatgggtca tcggccaatg gatcagctat
    13801 actgttgttc tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta
    13861 tggttaaagg gagtctgttt ttttctggcg ggcatggttt acggaatagg tatgaccgtt
    13921 tttgatacgc tgttatatcg tttgcctcaa ccttggatct attaccttca aggggtgtcg
    13981 tttgatttga tgcacagtat aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc
    14041 aaacgttttt acaatcattc aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc
    14101 aatgagtgtc agtatactca ttttgacaag ttgcagtaca acctatacac aatcggcttc
    14161 caaccaaaag gaggaagtaa aaacatcgca aaaagcaaca atcacgttac aagaaaatgg
    14221 tcaaaacttt tcggaaaaag aagtgttctt caaaaaaggc gatgatttac tgagtatttt
    14281 gaaacgaaat tttgaagtca aagaagataa cggcttcatc acttcgcttg aagggcactc
    14341 acaggatgag aaaaatcaac tctattggat gtttactgtg gacgggaaaa gtgctactac
    14401 tggtgccaaa gagatcaaat tgaatgatgg acaatcggtc attttcaatt tgtctaaact
    14461 ataagtttca taaaaaactg ttaaagggcg aatattgcca taattcagtg agttcagttg
    14521 atgaaagaag gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc
    14581 tgctctaggg gctaatattc tagttgctat cagcaaattt gtcggctatg caatcagtgg
    14641 cagtgccgcc atgctaaacg aaagcatcca cagtatcgtt gattgcagca atcagatttt
    14701 gctgttattc ggtgataaac aagccaataa aggacagagc gagttgcatc aatttggtga
    14761 aggacgagca aaatactttt tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg
    14821 ggcacttggg gtgatggaag cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa
    14881 tccgatcatt gttatagcaa tactgttatt tggtttggta atcgaaggaa gttcgttacg
    14941 cgtggcaatg aaagaaatca aagaattgaa tactgaaaaa ctttccacga tgcgcttttt
    15001 gcgcgaaagt cgacacagtg aaatactgat tatttttacg gaagacttgt gtgctgtaat
    15061 cggtttggtc ttggctttag caggaacaat attgacttat gcaaccggga ttgctgcatt
    15121 tgatgcgatc agcggattgc tgatcggttt cttgctgatg ggagccgcca ttttcctagc
    15181 aaaagaattt tatagtctga tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat
    15241 caagttagct tttgaacgtc ctgagatcag tcggctgatc gatataaaaa cagttcattt
    15301 aagtccaacg gagattttag ttgcagcaaa agcagatatc gttggtaaca aagagaatga
    15361 agcttactcg gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa
    15421 gatgtatata tacattgaaa cagataaata tgacccaaat tattctcgta aataaaacgt
    15481 ttattcatga attgaagcaa aaatgagaaa aatataatct tgtttttaaa taaaacggct
    15541 acaatttagc gaatttatgt tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga
    15601 tattttaatg aacaaaaaga aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc
    15661 actttgtata agctatatac cagtaggatt agcttgcgga gtattgttgc aaaaagttgg
    15721 atttgatcct ctcttatcag gactgttatc gattctggtc ttttccggtg gcgcgcagtt
    15781 tttagttgca tctatgctta cgacgcaagc atcatttgca acgacattat tgatggtttt
    15841 ctttttagaa ttacgctata cattattagg agcaagtctt gccggtttta tgaaaaaaga
    15901 aaagcggacg tttttggctg tcttttctca atcactgaat gatgaaaact atgcggtgaa
    15961 ctacttaaag ttttcaactg acccttcctg gaataaaaga aaggctttat atgtcaattg
    16021 gttctcaatg actgcatggg ccggtagcaa tatgatcggt aattttttcg gctcggtgat
    16081 tcgagtagat gctgaccttg tccattttgc tttgacggcc atgttcattt tcatgtttat
    16141 catgcagatg aaaagcgcac tactgatctt tacaggttta ttctcaggcg tattaggggt
    16201 cgtattcatg gtattattcc aaaatacttt cggattgatt gcagcaacag tcattgcttc
    16261 atttgccgga tttatgctgg agaaggcatt caaaaaagaa aaagctaaga aactaagaga
    16321 ccgcggtaaa gatgtcaatg agccgctctt caacttcccg gaacaggagg ccaatcacca
    16381 tgactaaact atatttgcta cttttagttg tttgtctgtt catcgtggca tatatcccgc
    16441 gcttgttccc gatgctttat tttactcatc gaaaagtgcc atcctggttc agtgactgga
    16501 tgaaatatgt accggtggca ttatttgcgg cgttagcttt taaagatgtt ttcattactc
    16561 atgaacatct ggatattgct tggaatatca aaatagctgc aatgattcta gtagcaggcg
    16621 tggcttacaa aacacgttcg atggcgttgt cagttttgac aggacttgcg tctgtctttt
    16681 tgctgtctat gctttaaact gttttatttt attaaaaaca gagattgcga cataagttgt
    16741 tcaaactgat ctatccgaac tgcatttgca acagtcaatt ctggatgcaa gtttattcgt
    16801 agggataaaa aagattagtg ttggcttatg atgcaagctc ttttttattt taggaattag
    16861 agagggattt gtccttctat gattttttgc tataataggg aagatgttta ttttatgaaa
    16921 gaatagttga gggaatttaa atgaataaaa aattgatgca aggaacattt tggctaacct
    16981 ttgctaatct tctatgtaaa gttttagggg tagtctattt gattccctgg ctaagtatga
    17041 tggggaacaa tcaagatggt atgcttgcta cgacattgta taatgttggc tatcttcctt
    17101 atggattgtt cctgatgctt ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag
    17161 ctgtagccac aaaagaagga gataacgctg cttgtcggct gatttttaga agtacgatca
    17221 atatcatgtt tgtcatcggt atcgcctctg ctgcactgat gtatttattt gctcctttac
    17281 tagccggaat cagtccgatt gctaacgtta ataatgggat actagctatc agaagtttat
    17341 gcccatcttt gattgctatc ccaattctta gtgcaatgag aggatatttc caaggaaaaa
    17401 atgatctgcg gccttatgga acttctttga ttatcgaaca ggctgtgcga gtgatcgtta
    17461 tcttggctgg tacttattac ttgcgcgtat tgactgacgg aaccatatta gaagctgtat
    17521 tgatcagtac agtcgcttct ttcttcggtg ggttagctgc gattatccat atgtatatcg
    17581 tcggacgcag aaaagacttc ttcgaactta aagatttttt gatttccagc cgttatttcg
    17641 aaagagaaaa ccgatctgcc tctgtctcta tcattcggga aacactaccg tttatttttg
    17701 tcggctcagt cattactatc gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc
    17761 actttttccg tccagagatc gcagatcagc aactggaatt cttattcagc cgtgcttctg
    17821 tcaatccaaa taaattgaca ttgatcctga tctccatggt aggaacagta gctataagca
    17881 gcttgccaat attaagcacg atgaagaaaa aagatcggat acaaatcgaa aaaacagtag
    17941 gagatagttt ttcgattgcg ttattgattc ttcttccttc attgactgga atgtctttat
    18001 tagctggacc actatacaca ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc
    18061 aaatggcttt gctagcttct ttgtttttct ctttattcac gatcctatct acgatgcttc
    18121 aatctttgaa tcaccatcgt tttgcgatcc gattaactgt agaagcaatt atcctgaaag
    18181 tcatcttcca agtaatcgga ttaggattag ttggcggata cgggatgagc ttatcaaatg
    18241 gcttagcatt cggtattgtc tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa
    18301 tagcaccttt agcaaagatc agcaattttt tcttaaaaac tttccgctcc accctgatta
    18361 tgcttgcagt atgttttgcc gttttctttt tgctgaatca gcgattgtca atggaatcaa
    18421 aaagctatgc aatgatttat tgtgtggctg taggaagtat tggcggctta gtatttttat
    18481 tttctcaatt cggaaaaaat gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa
    18541 gcaaagaata ggttaactaa tcgttttttg ctgtcacttg aaaaacggca actcgtatga
    18601 ttacgcttgt cgtttttcta ttttccaatt ttagtttcat atctaatctt acttatgata
    18661 tagttataag gtctggaaaa attctttgaa acgaggaaat taaacacatg aaacttgcag
    18721 tagttacaga tagttcggca tatctgccgg aacgtatcaa gaaccatccc gacttattcg
    18781 ttattccaat ccctttgatt atggatggga aaatttataa cgaaggaatc gatatcgaag
    18841 cggatgaata ttatggactt ttaaaaaaca gtaaagattt tcctactact tcacaacctg
    18901 tcgtagggga agttttgggt ttatatgagg aattgaaaga gaaagggtat gacacgattt
    18961 taagtatcca tctttcttca gggatttctg gctttgtcaa cacattattt gcgatgaaag
    19021 atgacatcag cggagtaacg gtcattcctt atgactctaa gatcactagt atgccgatgg
    19081 gacacatggt agaagctgcc ttagacttaa atgatgaagg tcagtccctt gatgaaatac
    19141 ttgcacatat cgatcgaatc cgagataata cctacgctta tctgattgta gatgatttga
    19201 acaatttagt acgaggagga cgtttgacaa atggtgcagc gctgattggc agcttattaa
    19261 aaatcaaacc gatcctgaca ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt
    19321 caagtaaaaa agcatttgct cgagctgaag aaattattgg tagaagaaat aacgaaatac
    19381 aaccgccagt caaactttac gtgatacatg caaataattt agaagtagcg attgcggaga
    19441 aagagaaatt gcagaaaaaa tatccaaatg cagaaatcga aattggtcat ttcggaccag
    19501 tcattggtac acatttaggt gaaaaagcca tcggcattgc catttcagcg caataattcc
    19561 ttttattcaa aaaacatacc aaaaatattc caacatcatc ttggactttc tacatgttac
    19621 taatgagcgt gtaaaagaac gagatggtgt ttttatttct atcaatgaag ctctttttct
    19681 ttgcaatcct cacttcaatc ctctataatg tcgcatatag tttagtaaat gagtaaacta
    19741 ctaatttact tatttactaa aaataaaagg atgaggagtt aaaataatgg aaataccaac
    19801 aaatttaaaa cataaaccag taatcgttgt agaagactat gataaagttg atggaagaaa
    19861 tgcattaaat acggatgcaa aagggctttc actgggcctt gctcagtgga atgatcgtgg
    19921 aaaagtcgat atctcaggca aggtatggcg acatactgga gaaaaatggt cacgacagtc
    19981 ggaagaatta ccaatcacac gtattttaga tttagcgatc ttagctgcac aaggaagttt
    20041 atacttccaa gatgcttatc gttatgaaaa attttatgat ccagaaaatc cagtcgtaga
    20101 catcattgga ttacaaggga accgaatgac ggtagaagta gataccaaaa atccaaagat
    20161 cgatgaagac atcattttgt attacgatac actacaaaaa gacggagaac tgattgggga
    20221 acgattccgt attttaaaac gtcttttaga tgagctagga tattaataga cagagatgat
    20281 caaaagggga agagaagcat ggagaatttc tcactaacag aaaaccgaac cttttcacgg
    20341 ctagaaaagc aaatgatctg gaagaaaccg aaaagccatc ggacaagtgc agaagaatgg
    20401 cggatcgcct ccgaaatcaa agggaattgg caagatcctg aaatgaaaat cagtaacgta
    20461 cttttggaag gagatgccgg gtcggggaag acacaattag ccaaagcact atctgctgat
    20521 ctgcaacttc cctatacaaa agtgacctgt tttgcagata tggacaaatc agatgttttc
    20581 ggagccttat tacctgtagt agattctaat aaggaacaag accaagaatt actagaagct
    20641 atttaccaaa cagatacact tgaagctgta ctgacactga tccagaacca ttacggcatt
    20701 gatccttttt ctgctaaaga aaaattagca gagctagttc aaaggatcga agctgattat
    20761 ccagatgcaa tccattacag ctattaccct tctgaaatcg ttcgagcgat tgaaaaagga
    20821 tatttattag agattcaaga gccgactgtc atccgagatg cctctgttct tgtggcactt
    20881 aactcagcgc tcgaaccaaa tgggatgttg aatttaccga caggaatcat tcgtcgccat
    20941 ccagattgcg tcattgtgat cactacaaat cgaaattacc aaggaaatcg cccattaaat
    21001 gaatctttaa gagatcggat gcaacatgcg gaaaaaatgg atcttcctgc cttagaagtc
    21061 atggcagaac gtgcgatggc gaaaactatg atccaaaaac aagaacttct cttaaagatg
    21121 gcagagatta tccgtttgtt ggatgaaact gcaaaagcta acgccatcaa aggtgtagca
    21181 ggaatgcgtt cttattttta ttgggcaaat acctataagc aaggacaaga tttactccag
    21241 tcgatttatc caaaagtttt gtataaatta tccactgatt cagatgaatt ggcaattctt
    21301 gaacaagctt tgtctgacag tggattgcta ctggaacttc aggacatatt gagaaaagaa
    21361 aaatggggtc atcctcaatc tgagcataca aaaggaagaa ccattagcgc cgaagaagca
    21421 aatgaacgaa acatcactga aaatgctgaa gaaattctgc gcttagatga aaagaaatcg
    21481 ttgtctgctg aagaaccaaa tgagggagaa gagaaacaag aagaaacaat agaaaaaaac
    21541 gagattcctc ctcaaatgca aaaagaacgc agtagcgata tcgaaggaaa aaatcaaaaa
    21601 gaacaacaaa caggagatga ttctcaagaa atgtctgctt ctgacatgga agcacatgat
    21661 aaggaaatca agaagcaact gaataaagaa gcgcgacaaa tcatgaagga aacgattcat
    21721 gaaaaagaag gtctcatcgt tcaccgacca gctttccaag gaaaaacgcc ggaagcacgt
    21781 gagctacaca tgcaagtaat gccaatagta gaatcactta gccggcaaat tttagagtta
    21841 ctggataatg aacaaacaga gacgtaccaa aaaggaaaat acgaaggtca gcggttcaat
    21901 gcaagcaaag tagcatatgg tgatttgaga aattttgaca aaaagaaccc gccacatgag
    21961 caaccttctt tagcggtagc tgtacggatc gatgaatctg ggtcaatgat acgggatgat
    22021 cggatcgaag cggcaaaaaa agcagcgatt gccattgccg aattttctaa gaaagtcgat
    22081 atcccattgt tgatttacgg agatacagca gaccgttcgt caagagaaaa gacttctttg
    22141 ttttcttata aagaatttga agacggattt caatggttaa atgaaaaatt agtcactatg
    22201 aaaccaaggc aaaacaatcg tgatggtgct gcacttcgtt tagcagcaga aaaattaaac
    22261 agacaatccg caacaaccaa actgttgctt aatattagtg acggtcagcc aaaagcgttg
    22321 ccggattata caggcaaaaa agcaaaagaa gatatccagt ctgttttgaa agaatatgaa
    22381 cgtcaaggaa tcctatttgt ctccgcagct attggccaag acaaagaaga aatcaagtcg
    22441 atctacggtg aaagtcgttt tgtagatatt actgacttga atgaatttcc caagcaaatg
    22501 attcaactga ttgcccgcta tctatgataa aataaagctc aacgaacgca cttagcagat
    22561 tcgttgagct tttcgtttct agttaccggt aacaaggata cttctaaaat agaatcggtt
    22621 ataatcaaga gggaccgtta aaggagtgaa acgatatgat acaagaacaa aaatggtgga
    22681 aagaagcagt aggctatcaa atctatcctg ctagtttcaa agatagcaat ggcgatggtc
    22741 aaggagacat caatgggatc cgtgaaaaac ttaattattt gaaagatctt ggtatcggct
    22801 ttatctggat caatccgatc tatcaatctc catttgtcga taatggttat gacgtaagtg
    22861 actatcaaga tatagaagag agattcggaa cgatggaaga ttttgattta ttgctaaaag
    22921 aagcacatga attagggatc aaagtgatta tggatcttgt catcaaccat agttcggatc
    22981 agcatcaatg gtttgaagaa tcgaaaaaat ctaaagaaag cccgtatcgc gattattaca
    23041 tttgggtcga cggggtagat ggcaaagaac cgaataattg gacatcgatt ttcggcggtt
    23101 ctgcttggga gtattctcat gaaactggac agtattatct gcatgttttt gcaaaagaac
    23161 aaccagactt aaattgggaa agcgagaaat tgaaagaaga actattcaat atgatccgat
    23221 ggtggttgga taaagggata gatggatttc gtttagatgc gatttctcat gtaaaaaaag
    23281 atgaatattc agtaaaagcc acagagaacc ctttctcacc ctttcaaaat gtctcaggaa
    23341 tcgaagaaca tttgaccgat ttgaaacatg tttttgaaga gtacgatatc atgactgtcg
    23401 gagaagcaag tggtgtcact gcagaagaag gaccacaatg ggtagggaaa gacggttatt
    23461 tcgatatgat attcgaattt gatcatatcc atatctggca gcaagaaaaa gaagggcaat
    23521 tagatgtcct taaattgaaa cttgcattaa gcgcttggca aacttcattg gatggtatcg
    23581 gatggaacgc tttatatatg gaaaaccatg atgttcctcg tgccgtttca gtgtttggag
    23641 atactcgtcc cgatttttgg gctatgtctg caaaagcaat cgctatgatg tatttctttt
    23701 tgcaaggtac gccatttatt tatcaaggac aggaaatcgg aatgacgaat atgccctttg
    23761 aatcgatcga tcaagttgat gcagtagatt cgaaaagact ttataaaagg ctgcttgcgg
    23821 aaggtaagac aagagaagaa gcattggata tcattcgtga aacaacgaga gacaacagca
    23881 ggacacccat gcaatggact tcggaacaat atgctggttt ttctacacat gaaccttggc
    23941 tgatcactaa tccaaatacc aaaacgatca atgtagaaca acaagaatat gaaccagagt
    24001 cggtcctgca attctataag aacatgatcc gcattagaca aaccaataaa ggtctgatat
    24061 acggttctta taaagaatat cttcatgaac atccacaact ttatgtctat gaaagatatt
    24121 tagaagatga acaatatctt attatggtaa acttaacaga atcacttgct gactatgaac
    24181 ttccaaaaga agcagatcag tcctggacat tactcttgtc aaattcctct agcggagaat
    24241 ttgaagctaa aggcatacta gcaccttatg aagctcggtt atataaaata aacaagtaaa
    24301 acttcctcgt taaagttcat tagttggact ttgcgaggaa gtttttcaga ttgctctgtc
    24361 aattactcat ttcaataatt cgctctagga ccaccgatat atttaggacg agagcgaagg
    24421 gctgtcacgt gagcttgtcc aagggttttc aaacgaggtc tgacaggtac ttggacatgt
    24481 ttgatatgca taccgattga tgtgtcaccg atatcgatac cggcttgtcc aactacgtgt
    24541 tccacttcga caggatcatt gaactggtca aaagcagcaa cagaacaagc acctccagca
    24601 tgtagtgcag gaacaacaga gacaatttcg aaattttttg cttctgccac tgttcgttct
    24661 actactaatg ctcggttcaa atgttcacaa ccttggacag ctagcgcaat ttctttagga
    24721 tccaacaact cttttaacgt acggatgatc cattgaccaa cttccgcact ggaatttttt
    24781 ccaatatgtc ctccaacgac ttcgcttgtg ctgcatccta aaacaaaaag atcacctttt
    24841 ttcaagtgtg cctcagccaa tatgtcatta actatttcag tcaattggtc ttttaattct
    24901 ttttctgaca acttcatttt ctaaaacctt ctttttcaat taatattgct tccgtgcttg
    24961 aatacgttga agacttgcag ttataggaaa ggcaagtccc attccaatca gattttggaa
    25021 gatgtttcct ggtatagaag ccactccagc cggccaatca tataaaatcc aaccggcgat
    25081 accatagccg atgatcataa ctaagcttcc taataaaagc cctaatcccc acatacgagt
    25141 agaagatttg ctgaaagaac cagcaacata tccttgtaac ccgtggatca ctaatgaaaa
    25201 aactgcccac tgcggatatc ctgaaatcaa atcgatcaat agaccactgg aagcaccaac
    25261 aactaatcca cccgttggac cataaagaaa agcagcggta tagatcccgg cctcgcataa
    25321 tgtcacaaat ccattcgtag ccggtacagg gatcaaaatt gttaatgaaa gggcaactgt
    25381 caacgcaatc agcatagcat agatcgttaa ttgcttagtt ttcatttctc attcctcctt
    25441 tttgaatacc gccagaccag acacttccgg tatgatcgtt catcagcacg ccattagaaa
    25501 tacaatgggc aacaaatttt ttactttctg ctactgcttt ggtaaaatct gcccctaaca
    25561 caagattcgc tgttattgca gaagaaaagg cacaaccggc cccgtttact gttgtagtgt
    25621 cgattttttt atgagaaaat acctcataat gatcaattga atagagaaga tccacagctt
    25681 catctccagc catccttgtg ccgcctttga tcacaatgtt ttttgttcct gaagcagtga
    25741 gttctttagc gagatggatc atttctgtta acgaagaggg aagagcttga ccagaaagta
    25801 aagcagcttc tgccaaatta ggcgtaatca tcgtaacaag tggaaaaagt tcttcgatga
    25861 gctgcgaaat atacaattga ttgatcacag cttcagtttc tttgaatgcc aaaacagggt
    25921 ctaaaatgat tgggatattt tgttttgttt ttatgaagtc tcgaacaacc atgattgctt
    25981 cacttgaatt caataaccca attttgatac catctaatga agtcatttta tcaatcgaaa
    26041 ctaactgtcg tttaagcaat tccaaagata tagattccag cataaatcgc tcattaatat
    26101 ctagtgcccc tatagaagtc aaggcgctta gtccgaatag accaaaattc tcaaaagttt
    26161 ttaaatctgt ttggattcct ccaccagcaa atggatcaga accgccaatt gtcaagattc
    26221 ttttcataac attttcctcc atgaataaga gtataacgaa attttcaaaa aagaaaaaca
    26281 gccaattggc atatttttat ctcatccaat ttaaaaaaac aaaaagaaaa gaaagaccaa
    26341 aatggatctt tctttcctta aatagttagg acttttgtga ttttaattct ttctcgattt
    26401 gaagccgttg cactttcatt gtagctgttc gcgggattgc atcaaaatca cggatgatag
    26461 ggacgttcaa gcgaggaagt tcatggactt gctcccacca tcgattccaa tccatttctt
    26521 tatcaggagc taaagcaata atcggttgtg gagaatgatt tttatccctt acaatcacta
    26581 cttctgccaa aaaatctaac gaatctaata aaaagtcttc aatagctaat gtactattga
    26641 tcgtttcgat caaatccact tgccgatctt tcaaaaatag gtggccatgc ttatccataa
    26701 aaccgtaatc tccgctatcc caccaatcgc cataaacatt ttcctggaac cgtgcatctt
    26761 ccttgtaata agtcaaagcc cgtcctttag acagtaattg tatatgacca tctgtgtttt
    26821 caggtaggac attcccaact gaatctgtaa tccgagcgct tgtcatgtct tccagtccaa
    26881 cgcccatatc tcttgcatca cttgttttca aactctctag tgtatgagcc ttaagaatca
    26941 ttggtccgca ttcactttgg ccatatactt gaagaaatac cgcatcctgt gccatcgacg
    27001 ttcgcaaaaa tgtcttcatt gtctggttgt ttatagcatc aaaagtggag tgatagtatc
    27061 ggatacccga aaaagcttct ggatgctcct tagcagtaaa acgccactga acaaagttat
    27121 tcggatgggt ctctagtgca attggccggt gagcttccaa catatgaaca acttttgaac
    27181 tttgtgcgtt tgctaaaggc atcataggaa atcccatgga catcaaagaa gagataccaa
    27241 tattgaatct ggaatgcact ggagagatgt gaaaagcgat taatttcttt tccgaaattt
    27301 ttgtgaaaac tgttttttgc catttcgtcc gccatcccat agaatgagca gaatgacaaa
    27361 taagtttagg gataccagtt gtaccagaag tatgggtcat ataagaaatt tcatttttgt
    27421 ctaaaaatga ttgagaaaca gacaaagcag gttgttccat taaat
//
LOCUS       DACENJ010000017        26101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.017, whole genome shotgun sequence.
ACCESSION   DACENJ010000017 DACENJ010000000
VERSION     DACENJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.017"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            79..1125
                     /locus_tag="IW748_000543"
     CDS             79..1125
                     /locus_tag="IW748_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1103475.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IW748_000544"
     CDS             1474..2106
                     /locus_tag="IW748_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1103476.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IW748_000545"
     CDS             2694..4133
                     /locus_tag="IW748_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1103477.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IW748_000546"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IW748_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IW748_000547"
     CDS             4895..5110
                     /locus_tag="IW748_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103478.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IW748_000548"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IW748_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IW748_000549"
     CDS             complement(5770..6681)
                     /locus_tag="IW748_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1103479.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IW748_000550"
     CDS             complement(6700..7704)
                     /locus_tag="IW748_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1103480.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IW748_000551"
     CDS             complement(7701..9824)
                     /locus_tag="IW748_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1103481.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="IW748_000552"
     CDS             complement(9829..12276)
                     /locus_tag="IW748_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1103482.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="IW748_000553"
     CDS             complement(12263..12652)
                     /locus_tag="IW748_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1103483.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="IW748_000554"
     CDS             complement(12712..13215)
                     /locus_tag="IW748_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1103484.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13452)
                     /locus_tag="IW748_000555"
     CDS             complement(13228..13452)
                     /locus_tag="IW748_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103485.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="IW748_000556"
     CDS             complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="IW748_000556"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1103486.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16212..16331)
                     /locus_tag="IW748_000557"
     CDS             complement(16212..16331)
                     /locus_tag="IW748_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1103487.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(16346..17530)
                     /locus_tag="IW748_000558"
     CDS             complement(16346..17530)
                     /locus_tag="IW748_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1103488.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(17789..18079)
                     /locus_tag="IW748_000559"
     CDS             complement(17789..18079)
                     /locus_tag="IW748_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103489.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="IW748_000560"
                     /pseudo
     CDS             complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="IW748_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19444..20118)
                     /locus_tag="IW748_000561"
     CDS             complement(19444..20118)
                     /locus_tag="IW748_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1103490.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20196..21536)
                     /locus_tag="IW748_000562"
     CDS             complement(20196..21536)
                     /locus_tag="IW748_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1103491.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(21619..21954)
                     /locus_tag="IW748_000563"
     CDS             complement(21619..21954)
                     /locus_tag="IW748_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1103492.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(21964..22338)
                     /locus_tag="IW748_000564"
     CDS             complement(21964..22338)
                     /locus_tag="IW748_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1103493.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22351..22665)
                     /locus_tag="IW748_000565"
     CDS             complement(22351..22665)
                     /locus_tag="IW748_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1103494.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="IW748_000566"
     CDS             complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="IW748_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1103495.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         7514 a         5454 c         4559 g         8574 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaacccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    13441 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    13501 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    13561 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    13621 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    13681 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    13741 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    13801 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    13861 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    13921 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    13981 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    14041 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    14101 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    14161 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    14221 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    14281 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    14341 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    14401 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt tcttgtgata
    14461 cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct ttttcagcac
    14521 tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata tacatttcct
    14581 ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta ccgcttttat
    14641 tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt tctacccaat
    14701 ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc ccttgcggag
    14761 ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct tttaatatcc
    14821 tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc tgttttatca
    14881 gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc acataatgta
    14941 gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact gacctattag
    15001 gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct aatacttgtt
    15061 ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt ctggttttac
    15121 catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca tgtggactgc
    15181 ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg ccaatatctt
    15241 taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg atatttcgat
    15301 atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg aaaatatctc
    15361 cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa tattcagcgt
    15421 gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg cttcactcct
    15481 ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa acaacataat
    15541 acttttctaa tatatacacg acttgagggc attcctccat tctcattaca agaatttctt
    15601 aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg tcagtttatt
    15661 ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata catatatatc
    15721 cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta tatggcattt
    15781 cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt tgcatttcta
    15841 caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata gaacattcta
    15901 accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc tggatagttt
    15961 tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct ttaccgagct
    16021 tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag gcgtttcaga
    16081 gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca aactttttcg
    16141 ttcttttatt agtgccttag cttttcacta aggaacgagt cgcacataaa ttttgtccga
    16201 aattcatctt gtcactttcc tttcttttcc attgctcgat ccgctgctgc acttgcattc
    16261 atcaagcaca tcacagtaac accaagcata ctgccaccaa ataccaaaaa aatatctttt
    16321 acaattccca tgacgattcc tcttttcata taatcaaatt ttcaattgcc atactttgtt
    16381 gctccaaaat ctttttatgt cggtctgata agcgtgcatt ctgtaccata tcctggatat
    16441 aggttgtccg attcaatcga tccaactcct tagccatttt ccacgtaggg gcgacttgtc
    16501 ggcctagcca attcaaggtt cgttcaatgg tatacggttt tggctcggaa gttaattgaa
    16561 tctctttccg attccgtcca ataaaacgcc cccattgttc attcaagggc cagttggttc
    16621 gccttttacc ttctacttta tccgcaaatc gcaagtaacg attgataatg gaaaacgtgg
    16681 tgctctcaat gctacgacct tttaacaaat cttgaatcgc atgaaaagca cggtcatttt
    16741 tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc taatggaatc cctaacttga
    16801 tatattgttc atagtctttt tcatacaaac agaaataaac ctcactcttg agactgccga
    16861 tatataaggt attgcccatg ccgtcctttt catcagcctt taaaagttcc ccggaacgat
    16921 aacttttaaa ggtacgaaac aaggagatac attcttcctt ctgacatttt ttcgttagct
    16981 caggtatatc caatagcccc actcgatcat tgattgccaa gtccaagcgt ttcatcacgc
    17041 caccggcctt tagacaatcc tcgaaaaagt cgtaccagct acgcttttga gcgagtaaaa
    17101 aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa aagcacacct ttatcttctt
    17161 catgagacaa catcaccaca atatcaccca tgacatactg ttcttgatag gagtaaaagg
    17221 cataatcttc atggagcata tactcaaatt tcaaatggag aatttcttga ataatttttc
    17281 gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa cagtaacgtc aaaggcaaat
    17341 cgggattaaa gctttccagt tggtcaaaaa ttttcctcat ggtttttata gatgcggtta
    17401 cgccaccatt ttctatctga tttaagtacg gccgactaag tccacaagcc gtagccaatt
    17461 tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa tagccgataa tctaaattcc
    17521 gttgtgccaa gttctcacct ccaaattcaa ggtgttaact ttttcaaatg agttaacacc
    17581 tatacaaatt acttactatc tttgatacaa caagcattct taatggttgc ttacttattt
    17641 tttgtgtttt tgtacccccc tgttagatac gggggtgaga catcatggtc ggctaacgcc
    17701 gtccatgaac aggcacagca ccacggagga tttctgcttc gcgaatcctc cgcggtcagc
    17761 gcctaacagt cagttgtgtt ggtttaggtt actcatctgt aataccattt acttccaact
    17821 ctgctttcat ttctaacaag tattgacgaa tatctggttc ttttgcttgg tccgcaatcc
    17881 gctcaaattc tgcaattact tgatttattt caattcgagc atccgtctcc tcattcatta
    17941 aatatggatc gccagcgact ttgacaccat tcttgcgaat atcaaatttt aaccgtcgca
    18001 attgttcttc taattctggg tctgactgaa tctttccaat cacccaataa atttctccaa
    18061 tcaactcatc ataaaccatg tacatccaac tcttttaatc ttctggcaat atcaaaaatg
    18121 accggaactg ttacagagtt tccggcttgc ttatacaact gactatctga attgacttga
    18181 gaatctcgat caaaagccca atcgggaaag ccctgaagtc gccaacactc tctaggtgtt
    18241 agcttccgaa tttgatactg atccgtgacg atcgcttgtt ggtctcctgt tagtaaggta
    18301 tgaacactct gttttcccag acgacctcgg cgacttgtgg aattaggaaa agccagattc
    18361 acactgtctt ctggcttcac ctcttgatat tctgtttcat tttttcgaat catgattttt
    18421 gtaccacctt gtttcgttga caaggtgggg gcggttccag taacttcata aactcgatta
    18481 ccttgttcaa actttcccgg taatagtcct gctacagaga ttccatccct tgctggcagt
    18541 gcgattttga ttccttcatc acctgttaag gttggcgcta cgccatctga accatagact
    18601 tgtccaccca ttcctcgatt ggaaggattg atatttacca aatttctgat gttttgatca
    18661 gatgttgaag acggtccaat gataggaaat acttgttcgg tacatgcgtc tcgagaatgt
    18721 ccgataagga atatccgctc ccggctttgg ggaatgtagg cctttgagtt aagcacctac
    18781 cactccacgt cataccccaa tccatccagc gttcggagga tcgtctcgaa cgtagcccct
    18841 ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata aaagcttagg tcggagaaga
    18901 gtggtgaacc gagcgatttc aaagaataaa gtacctcgag tatcggaaaa tctttgccgc
    18961 tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc aaaggatgtc cactggtccg
    19021 agggattgaa taaactcatc tgatacactt gtgatgtcat gcatttccac ctccttactt
    19081 gtatcatgaa ttgctttata actacgtcta gcaaatttat caatttcaca aaaaccgata
    19141 caatgatggc tcgcctgttc catacctaaa cgaaagccac caataccggc gaataaatct
    19201 aaaaatctca tacattacct cattcattgc aaagaaaaaa gccaagaaca ttttgctctt
    19261 ggctcataca ttattaattt gtcctactat tttttattta atccaactaa tttccgttgt
    19321 ttttcattgg cctgatcggc tacatattta atcaccgttt tgaactcttt tgcagtagca
    19381 ttttccgata aatccgcaat gctcttattg tatttcttta caattttcgt tacttgttct
    19441 ttctcagtca tgtaattgaa cctccaaatc tttcctacta taccctagtg gtaaaaataa
    19501 ttctagatta tccgtagcat aatgaacttc ggaaatatta ttcgtataat cttttattat
    19561 gatatgaagc aatacatctg gatgtttact ctttactttt gcaatgttct ctcttgattt
    19621 gaaaaaagcg ttaataaatg cctttttcgg cacatgacgt ccctctgcaa tttcacgttc
    19681 ttgtgtaaat ttccaagcga ttttagggtc ttgataaaca tagaaaatcg tcactcgaaa
    19741 atgttttttc aacgcccgcg agatattttt ttcagcactt aatattgcaa aagttgcatc
    19801 cagaataaaa gaatagccat gatctaagac atatctaaat gcctgctcta ccaaccaaga
    19861 agatgctttt tgaaagagac tagagttttt tccattataa tcaggaaact gggtgcgaaa
    19921 gtaatccgca tcaataatca catggttatc ataattctca gctaatccaa tggcaacctc
    19981 tgattttcca gccccaggac tccctgccat aaaaactgcg tctcgtccaa cttcggggga
    20041 tttccctttg ataatataag aaataaactt atccttattt gcttttgcat actctaaata
    20101 agaatcatta ctttccatat taattatccc ctttcttcct actatataaa agtattatac
    20161 gttacttgaa gtataaagtc agtattgaag atcactcaat cattcatctt ttctaattta
    20221 ctaattgaat ctaagaaatc ataccctttt ggtacaagtg gcgtataaaa ctcactaatc
    20281 acactgccac ctgtatccac ataacctcga cctttgatgt gcttcataaa gaagtttttt
    20341 tcgacctccc caaacatcat ggaatatccg agttcactca tacgacccaa agccacgcga
    20401 aagttgaatt gatcgcggat tccgtctcct aaatatttcg catccggcct ttggcaagcc
    20461 aaaatcagaa agaaaccaga ttgacgaccc aacatcacga tttgtttgag cttattcaaa
    20521 atcaccgcac tttctttcgt cgttaacatc tccatataag ccacgtattc atcaaagatt
    20581 aaaaagttgg gtggaagtcc aagataggca taattctctc ctgttttgta gttagacatt
    20641 tctttcattg ccttgctacg agccatcata cgttcataga aatcttccac acaagcagaa
    20701 atttcttcct tttgtgaata aacatgaggc atcaccgtac ctaaatcagc taaatctgca
    20761 tttttgggat cgaggataaa caattctgca tctgacttca atagtgcttc gataatggtg
    20821 aggaggaaat aggtcttccc tccacccgtg ccaccagcga ttaacatatg gggtaaagaa
    20881 tcataagccc atacttgatt tttcatcaat cgcagagtcc cattttctgc cactacttcg
    20941 tctatcccaa ttcgattggc gatcatatca tacagtaaag tatactccac ataggagtct
    21001 ttgagttcct tttctactaa ctcacaatac agtccgcttt ctaacttctt ttccaatttt
    21061 aaaagctgat cttggtattt cccaagtgaa atttgaacgc gaatagacag taatccatct
    21121 ttcattcgat aatagatttt ggggaagtaa ctgatagtct ctctggttcg gctactattc
    21181 aaatctttga aaaagccttc actttgagtt tgtttcactt cataccaatg gttctctaga
    21241 atcatccgag ctagcttttg tcgatgccac aagcttcgcc aatgatcaag aaaaaagtga
    21301 atataaagca atatcatagc tcctgaaaaa gcgagacttc caagcagaca tactaaatct
    21361 tttatagacc aaacgatttc aatagtagaa agtgatagct tagaaatatc aaattgtcta
    21421 aactcttgaa aataaaagac gctcatccaa ccgatgaaca gagcgagtag ccagccaaga
    21481 acaaagtgat aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt gtacattttg
    21541 atggtttcca cctccaatcg aaaaagccca ataggatttc attaggcaat ttcaaaaagt
    21601 tatgctagtc atttactgct acgaggaggg gagttgtttc gtttcaacca attgatttaa
    21661 aaagccttca atagcatccg gcatctccac accaatttct tccaaaatgg attctgcctg
    21721 ccgacataag ttttcattta cttcgacaac tataaagaaa gactcttctt ctcctaacca
    21781 gtctagtaca tcagagtctt cttcactgtc gctattttct ccaagtaaac cttgatgtat
    21841 aatcagaaac attttttcat cagaggttaa tgctttaacg agttgttcct cagtcagctc
    21901 gccttttcca tattgatcga gacaatctaa aatatgttca acattaattt ccataatctt
    21961 cacctatttc tttctttcat tttggttcgt aggatttcct tgattcggct gccgcttcaa
    22021 gactaaatca tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa aaacataatt
    22081 ggcaacggta tccacgattg gattgaccaa ctctacctct gcatcataag caaactcgcg
    22141 taagggtaca ttagctggga tactaacttg aatcattcgc ccttgtgcag aggattttaa
    22201 attataggtt cgttctttta cctcattacc aattgttcca tcctcatttt gcttccgtac
    22261 ttctcggcgt aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat ctacgacaat
    22321 accttctgtt aatctcatct tcttttcctc ctatgctttc actaaatcat caacatacaa
    22381 cacgtaatca gtaaacccac gattctcaat ctcatagcct ttggcaacca aatggggatt
    22441 cacggctttt agcggttggt cttgatcaaa atccttttca ccggcttccg cagctactac
    22501 cacctcaata tcatcggccc gttgcttact ggaatacaaa tgataactgc gaccaatcac
    22561 cgtagtattc cgtccatatc cttctgttat aatttctcct tcaccggcat aacttaactt
    22621 tccaaaggtt tcagccatat ctggcacgac aaatttcaat tccatcttct tttcctcttt
    22681 tcattgttag ttttggatac aaaaatagcg cgactacttt tttaggaggc ggtcgcgcac
    22741 gttggttctt aggatttact tgtatcatct catcacctta gttccggcga ttccagaaat
    22801 aatagcccgc cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca ttggaatttt
    22861 tctccccggt ttgagggaag gtcttcactg tgggactaga ttcttgagag ccacttggaa
    22921 cagttggtgt ttccggttgt ttcggcgtac ttagtatggt tggaacttct tttggtgtta
    22981 aggtttgaga tttttctttc aaatcttcat tgtgttttcc attgatattc ccatctttat
    23041 cgtaattgat ttccgcaaaa gtaaacttgg ttccttctgg atattttccg gtatctactt
    23101 ttttcgcaag cacagttttg gtgaattctt tatcatttac ttcataatca atcttgccag
    23161 atttccaaat ttctttgttt gtaccatccg gtaataaagc atacagaatt gtttcgaaag
    23221 cttcttttga gccatccagt acatcatgag taatcgacac atcatcaaac atatccacca
    23281 catcaccatg agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt tggatagaaa
    23341 catggtgttc cactgtacaa ctgacagttt gggcttggtt attcaagcta acatccttcg
    23401 ctactggctt atcgcctgct tcaaaagctt ccttattttc atacacatag ttgaataaga
    23461 caatagtttt atttaaaact tgttcggcgg ttaatttatg ctgaattttc caagttcctg
    23521 ttttctcttt gttcgccaac gttgcgcttg tttctgcaat caccactggt ttggcttttt
    23581 catctttttc ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct accacatacc
    23641 agtcttcttt gatttcatgt agattgtaac tcaatttatc aactagctcg gtagaatcca
    23701 gagaagttaa ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa ctatcttctt
    23761 cctcaggcaa atcatagagc attaaacggt tcaaactaac agaaaatgct ttgttcgtca
    23821 gcttctcgta aggaacggtc accgttttaa ttggttgttt ttggtcttgt tcggtaatgg
    23881 taaagacata ctcactcttc acaaagtctt ctttgttttc cttaaaagta gaacggattt
    23941 ctaatggcgt aatcttttga aaaccttctg gggcttctac ttcttcaagt aaataatccc
    24001 cataaggaag attttcaaac ttgccataac catcaaaccc taattgctcg ttataagctg
    24061 tggtcgcttc atcttcggca cctgtgattt cattggtccc ttccaatggc gacactttaa
    24121 aggtcagatc attgaagcca gtttcggtag tgcccgcagc tgatccagca aatttaaaga
    24181 aatcaaagcc aaagcgaatc acttgttctt ttgccgtaac atttcgggta atcacggcat
    24241 ttttttcgct atcatccacc tttttgatgg atacaggata ctttgtttca tcaaggctat
    24301 atccttcggg cgcttttgtt tcttgccaat aatactcgtt gatagctaaa tgtttaacgg
    24361 caacttggtt cttttcgtct aaagtcaaag tgaccgtttc atcagaacct ttcgttccct
    24421 tcgctaactc tggtttaaaa gcctcactcc atttaacagc ttttccatcc ttagcagtaa
    24481 acaaggtgta ttcagcaccc tcaaagactg ccttaccttg tgctttatcc ccagtatctt
    24541 tgtcttcttt cgtcaaagtg gtttcgccag tgacttcttg attttgccct tttacgttac
    24601 tggtaacaag ggctacggtt tgattggcat acttcaattc gacttttaca ggtttgaacg
    24661 tattcacgaa cccatgacta gctttagttt cggtcacata ataggttccc aattccaacg
    24721 cattagcaat ctcttttggt gtttccgcat gaccgttttc atccgtggtc atttcttgaa
    24781 cgatttcacc ggttggactg tctttgcgaa tggcaaacgt attaccagct aaggaataat
    24841 tgtcattcca aagatcactc cctgtttcaa ctccgctttt atccagaatg atttgccctt
    24901 tttcccgtgc gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg gtggtagtca
    24961 tgggggtggt atcaatcgta taaggcgccg gtactgattt ttcggtaatg gttacttttg
    25021 taccatgagg aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc acgtcttttg
    25081 caggtaaagt ttttccaaag tctaaatgga aaaccgttcc tggtacaaca tcacccgatt
    25141 ctttatcgac ttttttgatc tttaaagaac cctccgtttc cacatcaatt ttgattgtgt
    25201 aggtattagg tttatcaatc gccccggcca ttaaagtctg ttggccagcc tttttatagg
    25261 cgacaggtgt tccggtacca gcagatttct taagcgtcag cacaccactt tcttttgaat
    25321 tggcatttgg ggtgattatc aactgattac cattcacacg ataatcaatg tttgcggcat
    25381 tttccacaac ctcatcgaac tctgacaaat tcagattatt cgtatccgtc acagtagtcg
    25441 attgccctaa tactgtttta gccgtactat tatggaaggt tggtttcttc tgataatcgt
    25501 caatggcttg atttattttt gcctcaatgg ctgcgatatt tacggcacta ccatctgatc
    25561 gttttattga gtgaagggta tacccattaa cttcttgcca gatcatcttc tgtgccacaa
    25621 tatgagtatc tgcatctgta cctacttttt tccataaaac ggaaactaat ttggctttat
    25681 cggacatatc tggtaacggg tttttctcat agccaggagt gaactcattg taaatcggga
    25741 ctcctggttc aatacagaaa atatcttgtt taaagccgtt gtacactgca taatgcggaa
    25801 cagtgacttc tgacattgga cgaccatcac taaaagttcc ttcaacagca tattgatgtg
    25861 ctaaatctag ttctacggtt accgtttgtg gatgggtaat ttcatcagca aaagtaatcg
    25921 taggagcaat tgctccaaca attgcttgaa cagccaaact caaaatagtt aatacacata
    25981 atttcttttt ttgtttttta tacatgaaat gttctccttt caaattaagc acaaaaaaat
    26041 aagatgctcg cttgaacatc tttgtttata aagtcttttt aaaattttta ttgactgaat
    26101 a
//
LOCUS       DACENJ010000018        24935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.018, whole genome shotgun sequence.
ACCESSION   DACENJ010000018 DACENJ010000000
VERSION     DACENJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.018"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            145..579
                     /locus_tag="IW748_000567"
     CDS             145..579
                     /locus_tag="IW748_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1103496.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            745..2790
                     /gene="parE"
                     /locus_tag="IW748_000568"
     CDS             745..2790
                     /gene="parE"
                     /locus_tag="IW748_000568"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1103497.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            2802..5252
                     /gene="parC"
                     /locus_tag="IW748_000569"
     CDS             2802..5252
                     /gene="parC"
                     /locus_tag="IW748_000569"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1103498.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            5529..7760
                     /gene="pflB"
                     /locus_tag="IW748_000570"
     CDS             5529..7760
                     /gene="pflB"
                     /locus_tag="IW748_000570"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1103499.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8004..8765
                     /gene="pflA"
                     /locus_tag="IW748_000571"
     CDS             8004..8765
                     /gene="pflA"
                     /locus_tag="IW748_000571"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1103500.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            8850..9776
                     /locus_tag="IW748_000572"
     CDS             8850..9776
                     /locus_tag="IW748_000572"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1103501.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            9958..10851
                     /locus_tag="IW748_000573"
     CDS             9958..10851
                     /locus_tag="IW748_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1103502.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            10985..11110
                     /locus_tag="IW748_000574"
     CDS             10985..11110
                     /locus_tag="IW748_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1103503.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11244..11876)
                     /locus_tag="IW748_000575"
     CDS             complement(11244..11876)
                     /locus_tag="IW748_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103504.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12024..12566)
                     /locus_tag="IW748_000576"
     CDS             complement(12024..12566)
                     /locus_tag="IW748_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1103505.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            12701..13090
                     /locus_tag="IW748_000577"
     CDS             12701..13090
                     /locus_tag="IW748_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103506.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13094..13858
                     /locus_tag="IW748_000578"
     CDS             13094..13858
                     /locus_tag="IW748_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1103507.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            13987..14934
                     /locus_tag="IW748_000579"
     CDS             13987..14934
                     /locus_tag="IW748_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1103508.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15064..15804
                     /locus_tag="IW748_000580"
     CDS             15064..15804
                     /locus_tag="IW748_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103509.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16095..16763)
                     /locus_tag="IW748_000581"
     CDS             complement(16095..16763)
                     /locus_tag="IW748_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1103510.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            16897..17184
                     /locus_tag="IW748_000582"
     CDS             16897..17184
                     /locus_tag="IW748_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103511.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            17600..19138
                     /locus_tag="IW748_000583"
     CDS             17600..19138
                     /locus_tag="IW748_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1103512.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19135..20100
                     /locus_tag="IW748_000584"
     CDS             19135..20100
                     /locus_tag="IW748_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103513.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20087..21409
                     /locus_tag="IW748_000585"
     CDS             20087..21409
                     /locus_tag="IW748_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1103514.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      21472..21576
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            21650..22393
                     /locus_tag="IW748_000586"
     CDS             21650..22393
                     /locus_tag="IW748_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103515.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            22408..23400
                     /locus_tag="IW748_000587"
     CDS             22408..23400
                     /locus_tag="IW748_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1103516.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            23412..24149
                     /locus_tag="IW748_000588"
     CDS             23412..24149
                     /locus_tag="IW748_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103517.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24139..24861
                     /locus_tag="IW748_000589"
     CDS             24139..24861
                     /locus_tag="IW748_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1103518.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         8626 a         4202 c         5205 g         6902 t
ORIGIN      
        1 taataagatc aatttcattt tttaccctct ttcaagttga aaactatcct ttattttagc
       61 atgaataggt aacaggaaca attgaaaaaa tgaaagatac aagtataata gatggaataa
      121 aaagaaaagc aaggggtggc agaaatgtct gtaaaaaatc caacacaaga aacgattttc
      181 aactatttgg aagaagcaaa aaatattgcc gtcgtaggct tgagcaataa accagaacga
      241 acaagttaca tggttgcaca ggtattgcaa gacaatggat ataaaatcat accagtaaat
      301 ccattattgg ctggtgaaga aattctaggc gagaaagtct atgcgaattt gacagaaatc
      361 gatgatccaa tcgatatcgt agatgtattt agacggagtg aatttttacc tgaaatagca
      421 ggtgattttt tgaaaactga tgctaaagtg ttctgggcac aattagggat tgaaaacaaa
      481 gaagcagctg agatgctaag aaaagcagga cgtacagata tcgtaatgga tcgttgtatt
      541 aagattgaac ttggtaagtt gaaagaaaac caaaaataaa aatgaccaca aagaagagat
      601 gatcgataga ggggaatgtc gaaaaagcgg ttaggagata attaaaattg atttctcgtc
      661 taccctcttt tttttcacga ccttttctag tatgataatc gtggtgcggt tttgccgcaa
      721 agtattattg aaggagcgtt tgccttggca aaaaaagtaa acaacgaata caatgactcg
      781 tccatccaag tattggaagg attagaggca gttagaaaaa gaccagggat gtacattgga
      841 tccactgaca gccgaggctt gcatcattta gtctatgaaa tcgtcgataa tgcagttgac
      901 gaagcattat caggttatgg cagtgagatt gatgtgacca tccatgaaga caatagtata
      961 actgttgcag atagcggacg tgggatgcct gtaggaatgc atgcttccgg aataccaaca
     1021 gttgaagtga tttttactgt gctgcatgct ggcggaaagt ttggtcaagg agggtacaaa
     1081 acttctggtg gactgcacgg tgtaggagcc agtgtggtaa atgccttatc gaaatggcta
     1141 attgtaacga tcgttagaga tggcgtagag tatcagcaga aatttaaaaa cggcggtaaa
     1201 cctgacggta ctttgaaaaa gataggaaaa acaaaaaaag caaacgggac gactgtccat
     1261 tttttaccag atgatacaat attcagtaca acgaaatttt catatgaaat tttagccgaa
     1321 cggttaagag aatctgcctt tcttctaaaa ggcgttaaaa tctctctttc tgatcttcgt
     1381 ggagaagaac cagtcaagga aatttttcat tacgaagaag gaatcaaaga atttgttgat
     1441 tatctgaatg aagaaaaaga tacattgaca ccagttgttt atttttcagg agaaaaagaa
     1501 ggtatcgaag tagaggtcgc ttatcaatac aatgacggtt attcagaaaa tgttttatct
     1561 tttgtcaata acgttcggac aaaagatggg ggaacccatg aagctggtat gaaagcggcg
     1621 atgacgaaat cttataatga atatgcgaga aaagttggtt tgctgaaaga acgggacaag
     1681 aatctggaag gtagtgattt cagagaaggg ttagctgctg tactttctat ccgtgtacct
     1741 gaaaatcttc tgcaatttga agggcaaaca aaagaaaaac ttggtacgcc agttgctcgt
     1801 accgtagtag ataacgtcat cagtgaacaa atgggctttt atttgcagga aaatagcgaa
     1861 atgagccaga tgttggtccg taaagcaatc aaagccagag aagcgagaga agcagcacgt
     1921 aaagccagag aagaaagccg aaatgggaag aagcgtaaaa aaggcgaatc tcttctttca
     1981 ggaaaattga cacccgcaca atcccgaaac cctaagaaaa atgaattgta tttagtcgaa
     2041 ggggattctg ctggcggatc tgctaaacaa ggaagagaca gaaagttcca agcaattcta
     2101 cctttacgag gaaaagtcat caataccgaa aaagctaaaa tgcaagatat cttgaaaaat
     2161 gaagaaatca atacgatgat ctatacgatt ggtgcaggag tcggtcctga gttttctatc
     2221 gaagactgta attacgacaa agtaatcatc atgaccgatg cggatacaga cggcgcccat
     2281 attcaagtat tactgcttac tttcttttat cgttatatga aaccgctgat tgaagctggg
     2341 aaagtatata tcgcgttacc gcctttatat aaagtatcca aaggacaagg aaagaagcaa
     2401 gtcattgaat atgcgtggac agatgacgaa ttggcagcaa tgattaaaaa agtcggcaaa
     2461 ggatatatgc tgcagcgata taaaggactt ggagagatga atgcggaaca attgtgggaa
     2521 acaacgatgg atccgacttc ccgtacgttg atccgcgtcc gtatcgatga tgctgcgcaa
     2581 gctgaacgtc gagtaaccac actgatgggt gacaaagtag aacctcgaag aaaatggatc
     2641 gaaaatcacg tgcaatttac gctcgaagaa gatggcagta tcttagataa aaaagaggat
     2701 actgaaatct ctccttcagt ttctaatgat ttattagatg aagaacgagc agataaaaat
     2761 gaaaacaatc agttatttga agtagaatag gaggtcacct gatggaaaat cgacaagaag
     2821 tacaagaatt aactttagaa gaagtgatgg gtgaccgctt cggacgttat tccaaatata
     2881 ttatccaaga acgtgcactg cctgatatcc gtgatggatt gaagccagtg caacgccgga
     2941 tattatttgc catgaacaaa gatggcaata cgtatgacaa aggattccgt aaatcagcaa
     3001 aatctgtcgg aaatatcatg gggaattatc atcctcatgg tgaccgcagt atttatgaag
     3061 cgatggtccg catgagccaa gactggaagt tgcgggaagt actgatcgaa atgcacggga
     3121 ataacgggag tatggacggt gatccacctg ctgcgatgcg ttatactgaa gccagattat
     3181 ccaagttaag tggagagatg ctagcagata tcgagaaaga aacagtcgat ctcgtgtgga
     3241 attttgatga tacagaaaaa gaaccaacag tccttccagc tcgataccca aaccttctcg
     3301 tcaatggatc gacaggtata tctgctggat atgcaacaga aatcccgact cataacttag
     3361 ctgaagtgat cgatggcaca atctatatga tcgatcatcc acaagcgagc cttgaaaaat
     3421 tgatggaata tattcctgga cctgactttc caacaggagg gatcttgcaa ggaaaagatg
     3481 aaatcaaaaa agcttatgaa acaggacgag gaaaagttat tcttcgttcg aaaacaaaga
     3541 tagaggcaat gaagggtaat aaacagcaaa tcgtgatcac agaaatccca tatgaagtaa
     3601 ataaagcgac cttggtgaaa aaaatggatg agatccgttt gaataagaaa atcgatggaa
     3661 tagctgaagt acgagatgaa agtgatagaa caggtctgca aattgtcgtc gaattgaaaa
     3721 aagacgccaa tgctcaagga attttgaatt atttgttcaa aaacactgag ctacagatca
     3781 attacaattt caatatggtc gctatcgacc atatgactcc gcatcaagtt ggactgaaag
     3841 acattttaag aagttatatc gagcatcgaa agcaagtcat aacaaaacgt agtcaattcg
     3901 atttagccaa agctcaaaaa agacagcata tcgtagaagg attgatgaaa gctttatcga
     3961 ttctggatga agtaatcgca acgattcgtg aaagtaaaga caaaaaagac gccaaaaaga
     4021 atttagtcga cgtctttcaa tttacagaag aacaagcaga agcaattgtc acattgcaat
     4081 tataccgttt gacgaacacg gatatcacag agcttcaaaa ggaatccgaa agcttgattg
     4141 cgcaaatcac agagttgaat aaaatccttt caaatgacaa agagcttttc tctgttatga
     4201 aaaaagaatt gcgagaagtg aaaaaaaatt attcttccgc tcgtctaacg acaatcgaag
     4261 acgaaatcga agaaatcaaa attgatacgc aagtcttagt ggcacaagaa gacgtaattg
     4321 tcagtgtgac gcgagaaggc tatgtgaagc gaacaagcct tcgttcttat agtgcttcca
     4381 aaccagaaga aatcgggatg cgagaaggcg actacttgct atactcaggt gaattgagta
     4441 cattggacca tgttcttctg atcacaaata aagcaaatgt gatctaccgc cctgtccatg
     4501 aactacccga tttgaaatgg aaagatgctg gtgagcacat ttctcaaaca atctccaatc
     4561 tgtctgtaga cgaatcgatt ttagctgttt tcccatatca aaaaattcaa gcagagaaaa
     4621 catttgtatt catcagcaag aatggattga tcaagcaaac acgaatgaca gagttcgaac
     4681 catggcgcac ctataagagc cgtccgctca gctgtatgaa gctaaaggcg gaagaagatg
     4741 aactgattgc tgtgtatctg aaagaagatc agccaggctt agatgttttc ttagtaacgc
     4801 atcaaggaat gggtttgcgc tatcctttaa cagatgttcc cgttgttgga gcaaaggcag
     4861 ctggagtgaa gtctatgaat ttgaaagatg gagataggat tatcaacggg ctgcttgtgt
     4921 tagcagaagg tgatacgccg atcgtaatcg ttactcagcg aggagctgtt aaacgcatgc
     4981 ttgctcaaga aatcagccaa acttcacgtg cgaagcgggg agtgacggtc atgcgtgagc
     5041 tgaaaaaaaa tccgcaccgt attatctata tgtcggaagg aagatcaaaa gaaataacct
     5101 tgatgaatca aaaagggcag cagttaacga ttgacccaac tgatttccct ataggtgatc
     5161 gaacatcgaa tggatcattt gcgatggatg agaaaaaagg tggagaagtc atcgaagtta
     5221 tcgatgctcc tgagattttc atcgagtcgt aactgttgta gtattttaat gaagagacgc
     5281 catctgtatt aatacaggtg gcgtctcttt ttcattcatt tttaataaat ccttatacgt
     5341 ttgcaaaaaa agatgaaagt gttcacaatg cttaaatgtt ggtatgtcaa cgttgattta
     5401 aatatatgta aataaaacat aaaaattgtt aaataaaaaa caagaaaacc cttgcaaaga
     5461 tagggtgctg tgttatacta atcacgtaaa aactgttaca taaaacagaa atttaggagg
     5521 tccatacaat ggagcaatgg aatggattta aaggtaccaa atggaaagaa ggcgtagaca
     5581 ctagagactt tattcaaaat aactacactg aatatcgcgg tgatgacagc tttttggaag
     5641 gcccaacaga agcaacagaa aaattatggg atgaattgca aaagttaaat gatatccaat
     5701 tcgagcgtaa cggtgtctat gatatggaca cagaaatcgt atcaactatt acttcacacg
     5761 atgcaggcta tttggataaa gatttggaaa aaatcgtcgg tctacagaca gacaaacctt
     5821 tgaaacaagc attcatgccg ttcggcggaa tcaatatggc taacaatgcg ctaaaaagca
     5881 atggctatac accaaacgat gaattgacac atattttcac tgactggcgc aaaacacata
     5941 atcaaggtgt atttgacgct tatactgctg aaatgcgtgc tgctcgtaaa aacaaaatca
     6001 ttacagggct tccagacgct tacggtcgcg gacgtatcat cggtgactat cgtcgtgtgg
     6061 cattgtatgg aatcgactat ttgatggaac aaaagaaaaa agatcatgat atgactggtt
     6121 atcatacaat gagtgaagaa gtgattcgtt tacgtgaaga aatcactgaa caatatcgtg
     6181 cattagcgca aatgaaagaa atggctgcga aatatggcta tgatatttct aaaccagctg
     6241 caaatgcaaa agaagctgtc caatggctat acttcggata tttagcagca atcaaatcac
     6301 aaaatggtgc tgcgatgtca atcggacgta tctcagcatt ccttgatatc tatatccaac
     6361 gtgatttgga taatggagta atcacagaaa aagaagcaca agaattgatc gaccacttag
     6421 taatgaaatt acgtatggtc aaattcgcgc gtacacctga atataaccaa ttattctcag
     6481 gattccctat ctgggctact ttatccattg caggtatggg attagatggt cgttcattag
     6541 ttacaaagaa tgatttccgt ctgcttcata cattaacaaa tatgggacct tcaccagaac
     6601 caaacttgac tgttttgtat tctgaacacc ttccagaagg attcagaaca tatgctagca
     6661 gaatcgctat cgaaagttct tcgattcaat tcgaaaatga taatcagctt cgtgaacaat
     6721 ggggttctga tgattgtgcg attgcttgct gtgtatcagg aacagttgtc ggaaaagaca
     6781 tgcaattctt tggcgctcgt gctaacttgg ctaaaacact attatatgcc atcaatggcg
     6841 gagtcgatga agtgactggc gcccaagttg gaccagaata tcgtccaatc acatcagaaa
     6901 tactagactt tgaagaagtg aaacataact tcatgaatat gatggattgg ttagcagaac
     6961 tatatgtcaa tacgttgaac atcattcatt atatgcatga taaatattct tatgaagcag
     7021 ctcagctggc tttgatggat tctgacttgc aacgcacatt tgctacaggt attgctggga
     7081 tctctcatgc ggcagacagc ttgtcagcga tcaaatatgc aaaagtaaaa gcaatccgta
     7141 atgaaaaagg aatagcagtt gattttgaag tggatggcga cttccctaaa tacggaaatg
     7201 atgatgaccg tgcagatgaa ttagtttcat ggatcttgga atacttcatg ggacaaatca
     7261 aacgtcataa aacttaccgc aactcaaaac caacaacttc attattgacg attacttcaa
     7321 atgttgttta cggtaaaaat acaggaaata ctcctgatgg ccgtgaagct ggaaaaccac
     7381 ttgcgccagg agcaaatcca agttacaatg cggaacaaag tgggttatta gctagcctga
     7441 actcaacagc taaaatgaat tacggctgtg cattggatgg aatttcaaat acccaaacaa
     7501 tcaatccttc aggtcttgga aaagaacaag atacaagaat cgaaaacttg cgtaatgtct
     7561 tagatggata cttctcaaaa ggagcttatc atttgaatgt caatgtattc agtaatgatt
     7621 tattgcgtga tgcaatggaa aatccaatga aatatccgaa cttgacgatc cgggtatctg
     7681 gttatgcagt taaattccgt gacttgacaa gagaacaaca attagatgtc ttgatgagaa
     7741 cttcacacga cagaatgtaa aaagttcgtg aaaaaagttt ttcgacttag cgtcgaaagg
     7801 acgttgaata gccatcgcat caaaatcgaa taatcagtat gcttatttga aagaaacgat
     7861 ttacaggggc aggacttgta tgttccgtcc ctgtaaattt tacacataac gaagcggcaa
     7921 ttgcgaaaaa ttttgttcag aaagcttcgc ttcgacagct aacttgtact taaaatgaaa
     7981 tcaaagaaaa gatgtgaact taaatggaag aaaaaacaat cggctatgtt cattctatcg
     8041 aaactttcgg ttcagtagac ggacctggac tgcgctttgt cgtgtttatg caaggatgcc
     8101 gcatgcgttg ccaattttgt cacaatccag atacttggaa tattggcgga gggaaagaat
     8161 acacagcaga tgaactttta gataaagcag aacgcttcag accttactgg ggagataaag
     8221 gtggtattac tgttagtggt ggagaacctc tattgcagat cgattttttg atcgaactat
     8281 ttaaaaaagc aaaagagcgg aagatgcata ctacattgga tacatgcgga aaacctttta
     8341 cgtatgaaga acctttcttt tcacgttttc aagaattgat gaagtataca gatttactgt
     8401 tatttgacat caaacacatc gataatgaag agcataaaaa actaacgcat tgggacaatg
     8461 aaaatatttt agaaatggct caatatttgt caaaaatcaa caaaccggtt tggattcgcc
     8521 atgtactagt tcctgagcga agtgattatg atgaatattt gatccgacta gacaacttta
     8581 ttcaaacctt gtcaaatgta gacagagtag agattcttcc ttatcatacg atgggaaaat
     8641 acaagtggga aacacttggt ttgaaatatc cattagaagg gatcgaacca cctacaaaag
     8701 aacgtgtcga aaatgctaaa cgattgcttc atacagaaga gtatacaaaa tatttaacgc
     8761 gataggtcgc atcggaaaga agataagtat tgcttctttc cgtttttttg ctattattaa
     8821 ataggagaaa tcacaaagga gcgaatgaca tgtcaaaaat tttagttttt ggtcaccaaa
     8881 atccagatac agatgcaatc ggggcagcca ttgcgttttc acacttgcaa aatgaattag
     8941 gtaaagaaac agaagcagtt gctctaggta cacctaatga agaaacacaa tacgcattag
     9001 accactttca gctatctgca ccacgagtag tagagtcagc taaagcagaa aatgcagatg
     9061 tcatgcttgt ggatcataat gaattccaac aaagtatttc agatatcagc gaagtaaata
     9121 ttttagctgt agtagaccac catcgtattg caaactttga aacagctaat ccattgtact
     9181 atcgtgcaga accagttgga tgtacaagta cgattatttt aaaactatat aaagaaaacc
     9241 aagtaacaat tcctaaagaa atcgcaggaa tcatgctctc agctatcatt tcagatacat
     9301 tgttgttcaa atcaccaaca tgtacgcctg aagatgaagc agctgcaaaa gaacttgcag
     9361 caatcgctga agtcgatcta aacacttatg gacttgaaat gttgaaagca ggaacaaatc
     9421 taagtgataa atcagctgcg gttcttcttg atttggatgc aaaaagtttc ccaatgggca
     9481 gttccaacgt tcgtatcgct caagtcaata cagtggacct taatgaagtg atggatcgac
     9541 aagcagagct agaagcagca atggaagatg aaatgaaagc aaatggctac gatctgttcg
     9601 tattagttgt cacaaacatc ttagacagtg attcagaatt acttgttgct ggtaaaggaa
     9661 aagaaaaagt agaagaagct ttccaaacaa ctttgaccaa caaccgtgct ttgcttaaag
     9721 gtgtcgtttc acgtaaaaaa caagtcgtac ctcaattgac ggctgtattt agctaatcaa
     9781 atggtagtat gaagcattca aaatgaatgg ataagcgatt gatataaaaa gaaacataga
     9841 acatactaaa ggccgaagaa atggaggaga ataacagtca attatgactc ttgttcttcc
     9901 tcctttttac gtaaaagcct tttaagtaaa ataaaacgga tagaaaagag aacatatatg
     9961 aactattatt caaaaaataa ggaaaacttt tatcaaaaac tgaccgatga tccgcttttt
    10021 tcttcattga ccgattatct atatgaacat cgagaacaag aaacgattct aagagaattg
    10081 aaaaaagaat tttcgcaaaa taaattttcc cattttcttg atttattgat cgatgcagga
    10141 ttaatcaaga gggaagaacg aagatatcat ttgaactttc caatatttga tcctaaagat
    10201 tacttgcaac aagccacttc ggctgctgaa accatagctg aacaattgaa gagactctct
    10261 gttgatgaac aaaaattagc gatgggagaa gttatttggg cgtattgttt cgaagatgaa
    10321 cggaaagaag cgtattttta tggagtaaga aacagccgag agacagaatt gctgaggaca
    10381 actgcaggaa atgagaagta ccgttttatc acactttcct ctatagaaca cttcccgctt
    10441 actttggcta attacttttt tgtacagaag aatcagcttc ctgtaacgaa ggcattcaaa
    10501 gaattagccg aacttattgg agacgtaaat gaatcgtact tttttgatca aatagaagtt
    10561 atcgttgaca ggatacgtaa gaataaatat aaaaatcgcc ggcctagtat tttccatcag
    10621 tctctacttg tgacgaatac tataaaggaa gaagaatcat tcacattaga attacctatt
    10681 gtcgaaaaaa acaattttga gattgaatta ccaacattgg atccttcatt gacaatggag
    10741 gaaatggcct ttcttaaacg acaaatcttt tctgagctga gcaagaaatt tataccgcat
    10801 gcattttctt atatcaaaga atatggaaca gttttagtga gtaaaaccta agagagcagt
    10861 aagcaaaaat tatactgact cttgcaggag gagcctttct cttcctttat ggttaaaaga
    10921 attatttgag tattttgcct atctaccacc tattagattc actacatggt ctggaaacaa
    10981 aggaatgcaa aaaaatggtg agaaatgtgg aatgacaaag gaagtagtga taagaaaagt
    11041 tagattttta aataatcaat attatgattc agtcaagtat ggcattttat gggaagaact
    11101 agctgattag attaggaagt ccatttgata atccagaatc actaagaaac gaatggcgag
    11161 tcattaataa tttatccaat cttgggttat tcttttagat tgaagtgttt aaaactaaaa
    11221 aagcatttat gaagtcgtaa tagtcaggct tcaaaatgct ttttattttg tttgtttttt
    11281 ctttgccaca taaatacacc tagactgacg atgaagagca tatccaacag atttcctgcg
    11341 acttggatcg tcgaagggtt ggtcagtggc attaaagcaa ggacaaaaaa ggaaaacaag
    11401 ctgtaaataa aacaggtgac aggaactaat cgaaagaaga gcggacattt caattggcta
    11461 aaaatgagtg aaaaaataaa ataagtaaaa atggataaaa tcacgactaa accattcatg
    11521 ataaagaaga aaagcgtatt gtgattaaac gaataaaaaa gaattgctaa aaaagaaaac
    11581 gttttaaatg caatcaatac gcttgaagct tcgtttaacg taccaggatg tcgatgaaaa
    11641 aaataggcac cgaaataaac agaaaacagt aagccgcaaa gagaagacaa aatcaaaaaa
    11701 aacggatttt cttcggcttc tgtgatatca gaatgatcca aaagcaacca gcctaatgca
    11761 ctagaaggcg catgtgaggt cttaaggtat gaataacata gaaaaaataa gatcgtcaaa
    11821 gttgcaaaaa aagagcctaa atgtaaaaaa ttagtggttc tacgttgaat ttccataata
    11881 tctccaaatt aaattgataa gtatataaag tataataaca aattgatgag tatatgtcga
    11941 tgttttttaa tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat
    12001 tagacagttt ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag
    12061 aaaccacctg aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct
    12121 ttgatcaggc gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc
    12181 cgctcacctt ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt
    12241 atacccggca aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg
    12301 tatcctaatg ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct
    12361 aacgcataga caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg
    12421 acgatataaa tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc
    12481 attgtcaaca tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc
    12541 atccccaaca tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata
    12601 tcaaaaaaaa tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa
    12661 tgattataat agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt
    12721 gttttgtgcc agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat
    12781 tcaacagcca attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat
    12841 tttaagcgtt tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag
    12901 ttttttgaat ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg
    12961 ttctctgtgc ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa
    13021 cctgttccag acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat
    13081 aggtgagtaa caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt
    13141 agaagttgtt ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg
    13201 atggcagaca agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat
    13261 tcgtgtgatc ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat
    13321 tccatcctat acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat
    13381 caagcattta gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat
    13441 ggggggaatg acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg
    13501 tttgatgggt tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga
    13561 atataattat gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga
    13621 tttgtcattg cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc
    13681 tgacgaaaaa atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc
    13741 ttacggaaaa gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaatacc
    13801 actaatggaa gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt
    13861 gaaacggaaa atcactcatg acgatagagg attttccgtt ttttattttg ggctataata
    13921 aactctgtta ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa
    13981 gagagcatgc ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc
    14041 tctcgagtca tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca
    14101 gcaatggctg aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg
    14161 tcgcagatca tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg
    14221 aatttgctga tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt
    14281 acaaatgatg ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa
    14341 aaagacttcg attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct
    14401 gaaggatatg attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat
    14461 gccttagaaa gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt
    14521 gaaatttcaa gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa
    14581 atcttgcggt ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca
    14641 gattttacag ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta
    14701 agagggatta taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat
    14761 ggggtatttt tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata
    14821 cgtatgggtg aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt
    14881 caaggttatc gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaagtctta
    14941 ttactatata tatatttatg aaattcatcg ctattttctt aaaataatca gcgcattgct
    15001 actgaaaaca atgaatctgt gctattattg agaaaagttc catttttgag aaggatggtt
    15061 tgaatgaaag aaatcagacg aaaacatttc cgttttcctg cgtacgatga tgagttaggc
    15121 gtaaaattaa ccaacgacaa gaagcgagta ttatttgacg gacaagaaga tcttttgact
    15181 gaacagaatc agtccccgtt tgaacagatg aaagaatttc atttcacgaa cacgaacacc
    15241 gagaaaatcc gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa
    15301 aatttggaaa agcataaagg aaaattaccg gattataccg gccattacca atcagaaatc
    15361 acatcgacag gacgaaaaaa actttttggt gattcctctc gttctgtcag acaaagtgaa
    15421 acaaggacag agtccaagca tactacacga aacgcatctg ttgacacgaa ggtgcgggag
    15481 cgttcttatt ttgttcctaa atatgtaccc gcatcgatta tcccagaccc tagagaaagc
    15541 aaaatttctg aggatcattt aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg
    15601 ttcgatacag aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca
    15661 ttcaatcgaa caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa
    15721 agccatggta tcttagaacg ctcattacaa ggattgatcg atgatcaagg aaactctttg
    15781 ggagaaaata gctattttaa ataagtgata tacaaattta tattcggcta cagttggaaa
    15841 ggaaacgctg cggcgtttcc ttattttata ctctatccga aagtttatat cgtatcctca
    15901 aatttttttg agtttaagcg aattttttct cccaataagt aagaaaataa tggcaaaata
    15961 aaaaaacaaa ctattttcgc agatcgtttg caaaaatagt ttattttttt ataaagaaga
    16021 ttttactgaa taatatcatt ttagtttaca taatgatatt tctgtaaatt aaataaagta
    16081 gtgaaaaata agaatcagtg ctctaactgt tcaagaggat caagtggttc aaatgtttct
    16141 gtagtctgtt ttgcttccaa ttggggcaaa acagatccaa tttccagata cttcacttta
    16201 ttcaatgtat aagatagaat agaacagcta agtaatgtat acagaaaatg tgtgagatcc
    16261 aaataaagca aggagacaac taaaatacca aaagtaatca agaaaagact tttgccaatc
    16321 ttaatatgaa aatatttttc aatggcttga taaatgatat ctgttccgcc tgtgcttccg
    16381 ccgaatcgaa agacgagacc gcaaccaatg ccagaaagaa tacctgtcat caaactattg
    16441 atcagtagac tatctacagc aaaatgtata tgaaggctct cccataaatg taaaaagaaa
    16501 actagcgcaa atgttccgac gatcgtccgg agaaaagcgt gttttccaaa aagaaacaat
    16561 gagataagca ataaaggtaa attaaaaagc agtgaagtga cagaaggagc gaatccaaaa
    16621 agattatgca aaagcaaagc aataccagta accccaccat ctgccagctt agatggaata
    16681 ttgattcctg tcacagctaa tccgtagatt cctgcgccta aaatgattcc gcacaattct
    16741 gtaaataaat ttaatttttt catttctagc ctcttttctt ttgaaaattt tcagcaactt
    16801 ctttattata ctgacttata ctcttacttc aagaggaaaa tgaaaaaaaa ttatttttgg
    16861 ctgtttcttt ttatttatca aataaggagt ggaaaaatgc gagcagcaga cttattgaca
    16921 attcttacaa aatcacccaa aaaaacagag tatcaaggag tttttttggc tcaagaacaa
    16981 aaactggtgc ctgtgacaat ggtccagata aataaagcaa atcaatttat ctttttattt
    17041 gaacacggca aagcaccact tgctatgaaa gacatctttc tttttttgat gaagaaccgt
    17101 gagaaagctg tttattatca gtcaaaagaa gaactcattc ctctttatgg agtgaaagag
    17161 atagaaaata aagtggtgat ataagtaaaa ataagaaaac aagacgttta tgatgtcaaa
    17221 acaaccattt tttacatttt tggcaaaaaa gtgtcatgat acagtataat attcgttgaa
    17281 caggccaata aaggtgttct cccccacgag atttctactt tattggctgt ttctttttat
    17341 taaaataaaa gttgtgtgtg taaaaaagat atagctgatt ccaagtgaca atttcgtata
    17401 gatttttatg gtattcgtag tgaaaaaacg agaaccaaaa agttgatccg actttgttgc
    17461 ttggattctt tttttgtcag catatttaga ggtatttttt tctttttttt gttcgtgctg
    17521 taaggttaag gcaagacaag ttagttggga aaaaggaaag aaatgtttat acttaaagaa
    17581 taacagaaag gaaggtcgca tgaataattt tatacaaaca ttaagtgaaa gaaaaggcga
    17641 gttgctggaa gctgcatttc aacatctgtc catttctctc atttctttgc tgattgcagc
    17701 actgattgct attcctttag cgatttgggc agccaatcat aaaaaaatgg cagaagtctt
    17761 attgcagatc acgggtgtgc tccaaacgat tccttcactt gccttattag gtttacttat
    17821 cccatttgta gggatcggaa ctgttccagc attgattgca cttgttattt acgctttgct
    17881 tccgattttc caaaacacgt atattggtct agctgaaatt gacccgtcta ttgaagaagc
    17941 agcggtagca tttggtatgt ccagaatgag acgcttgctg aaagtagagc ttcccatcgc
    18001 attacctgtc attatctcag gtatccgaac tgccttagtt ttaatcatcg gtacggctac
    18061 tttggcagca ttgatcggag caggtggatt agggacattt attttgttag gtattgaccg
    18121 aaatacacct gttttgacat tgattggtgc gatcagttct gccttgcttg caattatatt
    18181 cagcggactg attcgctggt tgcagcataa gaaacctcga tattccatca ttactttagc
    18241 agcagtggtg tgtttatttg gcggcgtttc tttatatcag tctgccttgt ttaaaaatga
    18301 aaccatcacg attgctggaa aattaggagc ggaacctgaa atcctgatcc aaatgtataa
    18361 agaattgatt gaagacaaca caaaaacaga agttgaatta aaaccgaatt ttggcaagac
    18421 gagttttttg ttcaatgcac tggatcaagg tcaaatcgat atctatcctg agttcacagg
    18481 tactgtccta gaaagcttgg tgaaggtacc tgaagcaatg aaaagtgctg atttgacaaa
    18541 agaaaaaaca tatgacgaag cagaagaatt attgaataag caattcgata tgacattatt
    18601 aaaaccaatg gcttaccaaa atacgtatgc cttggcagtc aagagatctt ttgcgaacga
    18661 acatcaactt aaaaagattt ccgatttggc agctatctct gatcagatca aagcgggatt
    18721 cacactggaa tttattgatc gttcagatgg atataaagga atccaagaag catatggctt
    18781 gaatttttca agtgtacaaa gcatggagcc aagtttgcgt taccaagcaa tcaataatgg
    18841 agatgtcaat attgtggatg cctattccac agatagccag ctcaaggagt acgatctagt
    18901 gactctagaa gatgatcgag ggttattccc agcttatcaa ggcgcaccgc tgatgaaaaa
    18961 agattttgct aaagaacatc ctgaaatcgt taaagcactg aataaattaa gcggaaaaat
    19021 aacagaggat caaatgattg atatgaatta ccaagtcaat gtcgaaaaga aaaagccgac
    19081 agaagtagca cgaatcttcc tgaaaaatga aggattagta aaggaggaag atcagtgact
    19141 aaattaatcg agttcaaaca tgtgcaaaaa aagtatgatg gaaagtatgt catcgatgac
    19201 ctaaatctgg caatcaaaaa aggagaaatc tttgttttgg ttggtccttc cggtagtgga
    19261 aaaacaacta ctttgaaaat gatcaatgga ttatctaaac catcagcagg agacatctac
    19321 ttcaaaggaa aatcgttaaa tgaatataat ctgcagaaaa tgcggtggaa tatgggatat
    19381 gtgctgcagc aaatcgcttt gttcccgacc atgacagtca agcaaaatat tgaagtcatc
    19441 cctgaaatgt taggttggga aaagcagaaa agagcagacc gtgtagatga acttcttcaa
    19501 aaagttggct tatctcctga tatttatcga gatcgtatgc ctagagagtt gtctggagga
    19561 gaacagcaac ggatcgggat catccgagct attgctgcaa gcccagatgt gattttgatg
    19621 gacgagccat ttagcgcatt agatccgatt tcaagaaact cattacaaga attggttttg
    19681 tccttacacg aagaattggg cacgaccatc gttttcgtta cgcacaatat ggaagaagca
    19741 atcaagctag gtgatcgtat tgcatttatg aaggatggag aaattatcca atgtgacaca
    19801 ccagagcagt tattgatgaa tcctaaaaac gactacgttc ggcatttctt tgatgaaccg
    19861 aaacagacga aagagtggcg agtagaagat ttagtcgtca atggatattt cttaaatgaa
    19921 ataccagaaa atgctcgtca acaagtttgc tttgatacgc ctatgaaaga agtatattcg
    19981 cttctttcgg tgtatccaag cattgtgata gtagaaaagc aaagaagtat tgggtcgctg
    20041 acaagtaaag aaatctttgc ttttttaagt cgagaggagg aaggaaatga aaacatatga
    20101 tgcaatcatc atcggttcag gtgtgagcgg cttaagtgct gcttatggat tgaaagaagc
    20161 aggtaaaaca gtactagtgg tcgaagaaga tctatggggc gggacttgtc ctaaccgtgg
    20221 atgtgatcct aaaaaagtct tgttaagtgc agtagaggca cgtaatcggg taaaacaatt
    20281 gagtggaaaa ggttttaacg agatcccaac tgccaactgg gaagaacttc aaaaattcaa
    20341 acgaacattt acggatccag tcccagaaag ccgaaaaaaa caattagcag aagctgaaat
    20401 cgatcatctt tcaggaaccg ctcgtttttt ggatgattca tctattgaag tcaacgaaga
    20461 agtcttccat gcggattact tagttttggc aaccgggcaa agacctacga tcttaccagt
    20521 ggagggaaag gaatacttga aaactagtgc agacttttta tcattgcctg tactgcctaa
    20581 agaaatcatt tttatcggtg gtggttatat tgcttttgag ctagctacga ttgccaatgc
    20641 tgcagggagc aaagtaacca ttgtccatca taatcaacgg ccattaaaag aatttgaggc
    20701 gtctttggta gaggaagccg tccatcaaat ggaagcatct ggcattcagt ttgcttttgg
    20761 tgttgaaacc caaaagatca tttcagaagg tactcgctat cgtttagtcg gaaaagagac
    20821 ggaattagta gcagatatga ttttttgtgc gactgggaga cagccgaata cagaatcttt
    20881 agcacttgag caagcgaata tcgtctttga taaacacgga attgcagtca atgattatct
    20941 gcaaacgagc aatccaaaaa tttttgcgtg tggagatatt gtttcaagga aaaccccaaa
    21001 attgacacca gtcgccactt ttgaaggaaa ttatgtagct aagcggataa cggatgcaac
    21061 aagcgaacct atcaaatatc cgattatacc tacgatcgtc tacgccagtc ctaaacttgc
    21121 tgaagtagga gttacaaaaa gtcacgcctc ttcgtcagat caagtggtag agatggatct
    21181 taccagttgg ttcacctatc atcgtgtcaa tgaaccagtt gctaaggctg aattgacctt
    21241 tgaccagcaa aattatttga ttggtgctgc agtgattagc gagcaagcag acgaattgat
    21301 cgatgatttg acactcgtca tcaatcaaaa attgactaaa aaagagttgg atagctatat
    21361 catggggtat ccgacattgg caagtgatct ctcctattta ttgaaataat cagcatgata
    21421 actaaagcgc ttgttgcttt ttccttcttg tgctacactt taaaaaatga atacctgttt
    21481 ctggggatgt cgtagacgac atgagagtaa gttgtgaact tagacccatt gaacctgtca
    21541 gttaatactg gtgaagggag aaacatagca gacgcttgaa gcgggttact atgtcgtgcc
    21601 catttcaagc gttttttgtt attcatatta gaagaaagga ggtaacaaaa tgaagaaaaa
    21661 tctttatcca gcagtcagta tggcattcat tttaattatt tggcagattg ttgtcatagt
    21721 ttggaaaata cctgccttta ttttacccgg acctgctgct gtattttctg cattagcggc
    21781 agatgcacaa gtattacttt cccattctgt cacgacatta aatgaagcag tgcttggttt
    21841 ggtaatagct agcgttcttt cttttattac tgccttggca atggattatt ggaagttttt
    21901 agaaatgtct ttttatccgt tactagtgat ttcacaaatg ttgccaatca tggtgttagg
    21961 gccattactt accctatggt ttggtttcgg tatgcttcca aaagtgatcc ttgttgtctt
    22021 gataagctat tttccgatcg tagtttcttt ttcggataca ttgaggaaag tatcaaaaga
    22081 gcaaatcatc tttttgaaaa cgatgggggc agatacaggg aagatttatc gaatatataa
    22141 aataccagcg ggtttatccg gcttcttttc cggtttaaaa gtggctgcta cttattgtgt
    22201 cagcggagca gtcgttggag aatggctaag cgctcaagca ggcttagggt attacatgat
    22261 acgcgtaaaa aacagctacc agatcgataa agtttttgct gcgatcattt gtgtcattct
    22321 attgagttta ttgctgaatg gcgcttgttc attattgaaa aaagggtatt atcgtatgct
    22381 ttatattttt taagagaagg ggatcaaatg aaaaaattat ggcttttatt accgctactg
    22441 ttgcttttat ccgcttgtgg cactgcaaaa gaaacaagta gcaaacaaga aataaaggat
    22501 ttgaaaaaag tgacacttgt tttggattat gtaccgaata ctaatcatac tggcatctac
    22561 ctagcaaaag aaaaaggcta ttataaagag gctggtttaa atgtccaaat catcgaacca
    22621 ggagataatt cgacaagtat tggtctagta ggtgcggaca aagcacaatt tggtgtaagt
    22681 tatcaagaag atgtcactta tgctcacgca gatggacaga atataccagt caaagcaatt
    22741 gctacagtga tcaaacataa tacttctgga ttcgccaccc tatcagacag taacatccat
    22801 tcaccaaaag attttgaagg aaaaacatat gcaggttggc aaagtccaag tgaagaagct
    22861 gtcttaaaag ctgtgatgga aaaagacggc ggggactttt caaaactgac aatggtagga
    22921 agcaatggag aagggccaga aagcttagga aaatcctcag atatccaatg gtactttgaa
    22981 ggttgggata tgatcaaagc aaaagaagcg ggtattgaac taaactatat cccattgaaa
    23041 gaattagatg aacggttaga ttactatact ccggtaatta ttacgaatga tcaactgatc
    23101 aaaagtgatc cagaattggt acagtctttt atggatgcca cgaaaaaagg ctatcaggaa
    23161 gctattaagg atccaaatga cagtgctaaa ctgttgcaaa aatatgcaaa agaaaatgat
    23221 cggacgtttt tagaagaatc acaagcattc ttgtcaaaaa attatacgga tgatcctgag
    23281 aattggggat tgatggaaga aaaagtttgg cgcaattata cagcttttat gcaggaaaac
    23341 ggattgatca agcaagatgt accttcgcaa gaactattca ctaatcagtt tataaaatag
    23401 gagtgataga tatgtcaatc caaatcagcc acttgaatgt agtcattgaa aacctgaaaa
    23461 tattagaaga catccatttc actatcccta aagatacctt tacatcgatt gtcgcgccaa
    23521 gcggagccgg taagtcaaca ttgctgaaaa ctttaacagg agtccagcct atgacttctg
    23581 gaacgatcaa agtagacgat caaaaagtaa ctggtctcca tacagcattt agttatatgc
    23641 cgcaagaaga tatgttactt ccttggctga gcgtctatca aaacgttacg ttatatcaaa
    23701 aaatcaatca tctaacaatc aatgaagaac aagtaaggga ttatctggat atctttgggt
    23761 taaacggcta tgaacatttt ttgccggaac aattatctgg cgggatgaag cagcgaacgg
    23821 ccttgttacg aacaatcatg aatccttctt cttacttgct tcttgatgaa ccatttggag
    23881 cgttagatgc tatgaccaga gggcagatgc aagactggat gttgaagctg cctaaaaaag
    23941 caaagcggac tactttactt gtgacgcatg atatcgaaga agcaatttat ttatcagaca
    24001 ggattcttgt gctttccgcc cgtccggcac atgtgattgc tgagattcaa gtgcctgaaa
    24061 aacgacggtc acgtgaatgg ctactgcagc aatcagagtt gaagcaaacg atttatcagt
    24121 tattggcagg tgaattagat gttaagtgat gctcattgtc atttagatgg gagtcagagt
    24181 ttagcactcc ttcagcagga acacacgatt ttgacgatca tcaattgtga ttcgccagaa
    24241 gaatggaagg aaaacagaca gcttgcagca agtaagactc aagccttaag ttacggtatc
    24301 catccttgga aagcagatag ctatacgttt gaacaagttg agcctttttt gaaaaaagca
    24361 aggatcatcg gtgaaatagg gttagataat atttggacga atgtaccgat gactacacaa
    24421 aaaaaagtat ttgagcgaca gcttgctttt gcagccataa atgagaaacc tgttgtactg
    24481 cacacaaaag gatgcgaaaa agagatctta aattatatcc aagcttaccc taatcactat
    24541 ttgatccatt ggtattcttc tttggactat caacaagact atattgattt aggctgttat
    24601 ttcagcattg gtcttgattt gaaaaagaat ccagcagtct ggcaactagc taaagcagtc
    24661 ccaatcgacc acttacttat tgaaacggat ggactacatg cagcagagtg ggctttaggg
    24721 gggatttttt cagaaaaaga ttatgtctca ctgcttgaag aacagttaaa gacaatcgct
    24781 gagatcaaag agctatcagt tgaagaagtt aaaaaaaata cttatcaaaa cttggaacgt
    24841 ttcattagat taggtaaata aaaaagagat gtgacacaac tagtgtcgca cctctttttg
    24901 gctttctttg tataaaagta aactgtttga ttaat
//
LOCUS       DACENJ010000019        24739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.019, whole genome shotgun sequence.
ACCESSION   DACENJ010000019 DACENJ010000000
VERSION     DACENJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.019"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            13..1014
                     /gene="ccpA"
                     /locus_tag="IW748_000590"
     CDS             13..1014
                     /gene="ccpA"
                     /locus_tag="IW748_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1103519.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLADRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEGFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(1351..3696)
                     /locus_tag="IW748_000591"
     CDS             complement(1351..3696)
                     /locus_tag="IW748_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1103520.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            3975..4877
                     /locus_tag="IW748_000592"
     CDS             3975..4877
                     /locus_tag="IW748_000592"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1103521.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            5090..6865
                     /locus_tag="IW748_000593"
     CDS             5090..6865
                     /locus_tag="IW748_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1103522.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6931..8517)
                     /locus_tag="IW748_000594"
     CDS             complement(6931..8517)
                     /locus_tag="IW748_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1103523.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQEMVEEIE"
     gene            8768..8992
                     /locus_tag="IW748_000595"
     CDS             8768..8992
                     /locus_tag="IW748_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1103524.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            9133..10884
                     /locus_tag="IW748_000596"
     CDS             9133..10884
                     /locus_tag="IW748_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1103525.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            10884..12668
                     /locus_tag="IW748_000597"
     CDS             10884..12668
                     /locus_tag="IW748_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103526.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            12994..13845
                     /locus_tag="IW748_000598"
     CDS             12994..13845
                     /locus_tag="IW748_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1103527.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVVQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            13942..15822
                     /locus_tag="IW748_000599"
     CDS             13942..15822
                     /locus_tag="IW748_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1103528.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEVEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAISGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(15862..16764)
                     /locus_tag="IW748_000600"
     CDS             complement(15862..16764)
                     /locus_tag="IW748_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1103529.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENKVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            16995..18398
                     /locus_tag="IW748_000601"
     CDS             16995..18398
                     /locus_tag="IW748_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1103530.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALNDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            18398..19831
                     /gene="ascB"
                     /locus_tag="IW748_000602"
     CDS             18398..19831
                     /gene="ascB"
                     /locus_tag="IW748_000602"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1103531.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            19976..20902
                     /gene="trxB"
                     /locus_tag="IW748_000603"
     CDS             19976..20902
                     /gene="trxB"
                     /locus_tag="IW748_000603"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1103532.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            21116..22843
                     /locus_tag="IW748_000604"
     CDS             21116..22843
                     /locus_tag="IW748_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1103533.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            22915..23208
                     /locus_tag="IW748_000605"
     CDS             22915..23208
                     /locus_tag="IW748_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1103534.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(23300..24160)
                     /locus_tag="IW748_000606"
     CDS             complement(23300..24160)
                     /locus_tag="IW748_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1103535.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            24252..24680
                     /locus_tag="IW748_000607"
     CDS             24252..24680
                     /locus_tag="IW748_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1103536.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
BASE COUNT         7826 a         4398 c         5302 g         7213 t
ORIGIN      
        1 gggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat
       61 gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgttaa accagcaaca
      121 agaaaaaaag tcttggaagt catcgatcgc ttggattatc gtccaaacgc agttgcgcgt
      181 ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca tccctgatgt cagcaatatg
      241 ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt
      301 atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa taacttactg
      361 gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga
      421 gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa
      481 gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa taaattggca
      541 aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc tatcaatgga
      601 caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa
      661 ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagc cgatcggatc
      721 cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg
      781 gatggtttat tagatcgtgg cgtgaaagta ccggaaggat tcgaaatcat cacaagtaac
      841 aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac
      901 gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa
      961 gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataataaaag
     1021 gacaaccatt cagaggttgt gagcctgccg tttaactctg cgttacaagg agcaatttca
     1081 aaaaacagca tttcatgttt gatgaatatt gtgactcgtg attcggagtt ggcaggagaa
     1141 caccgtttat tcgggaaaag gggttatgac aaaattttgt tataatcctt tttttgtgtc
     1201 ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata
     1261 gatgctgcat tttgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca
     1321 caatctctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg attctttggt
     1381 tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat agttttcatc
     1441 ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg cccatagcga
     1501 ttcaactgtt tttccaggtg tttggatgga ttttttattg acagtaactt ttccaggtgt
     1561 ttgaccagta aatccagata cttttttctt catcacatca gatgataatt cgaatttctc
     1621 atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat tggcaagata
     1681 attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt gccctgccca
     1741 gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt attcattggt
     1801 agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgagatggc
     1861 atctttgaat ggtgtagtga tttttgcatt gatcacactg cgcatcatgt cattcatgat
     1921 cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat aaattacatt
     1981 tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc cgccgtttgc
     2041 taaagcctga aatccattgg tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc
     2101 agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt agtgctcgta
     2161 agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat aagccgggat
     2221 attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc cttcgttagt
     2281 agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg cataatctga
     2341 tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa caagaactgg
     2401 tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt gattctgact
     2461 gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca tcaatacatt
     2521 tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat tttctacgcc
     2581 attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat atcctttatt
     2641 ttggattgtt tgctgtgcct tttgataata agcgtcactg accgatgaat cagacatatc
     2701 agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat tcatcacagt
     2761 ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt
     2821 aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt cacgatacat
     2881 actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt ttagcgcacc
     2941 tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga tataagcagc
     3001 ttgcggcagc gtcacatctt ttgcatctac accaaagatt cctcttgcag cttcctggac
     3061 tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca aatacgtagc
     3121 aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga tctcgtttgc
     3181 tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt gctgtgtcaa
     3241 agttgatcca ccagaagagc caataccagt agcttcagat actaacgctc gtaaaacagc
     3301 tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga taattgcagt
     3361 tttcaataaa ggtgaaattt tatcagatga aatagtcgtg cgcatcagat ctgtctggat
     3421 ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa tgtttcctag
     3481 ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata
     3541 gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag taataaaaac
     3601 agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt ctttttttct
     3661 tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt
     3721 tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt ttttggcagg
     3781 aatttctttt ttaaaggagt taaaacgtga gaaaatgatc ttttttataa aagagttctt
     3841 aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga aaaatcaatc
     3901 attttctttg tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa
     3961 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
     4021 taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg
     4081 cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt
     4141 acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg
     4201 ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg
     4261 ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg
     4321 caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag
     4381 aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa
     4441 catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta
     4501 cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg
     4561 taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg
     4621 attctaaaaa tccgcaaggt tcgcataaag accgacacgc caatattggt tttggaacaa
     4681 tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa
     4741 tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca tatggaaaag
     4801 aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag ttattagcag
     4861 aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc
     4921 cttgtccttt tttgtttaag aaacaaagga gtgctttttg cagtcttttt catcatgcat
     4981 cttctccccc ctctttttaa atttagacaa acagggtata ctggtagaaa agtaaaaaaa
     5041 cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac
     5101 tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc
     5161 cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat
     5221 gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa
     5281 atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt
     5341 agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt tcagtatttt
     5401 tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt atcttctttt
     5461 aagtttctat ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg
     5521 aacgtatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt gtttactagc
     5581 tatttattac cgcaaatacg gttctagcag aaaattgctg ccatggtttt tagggctttc
     5641 tgtcgctttt cctttctttt ggttcattcg cgaagattct atctggcttc ttccttttac
     5701 tgtagtagct ttgggtatca gtttcgtcca gatcttgcta gggcaagact tttctttcaa
     5761 agaagtaaag aagttaaaaa aacgactctt tcttctctcg aaacgggaat ggctgaaact
     5821 ttcattgttt ttacttccta tagttggctt ttccatcgtc tatcagtgga tcgaatataa
     5881 aaatgagaca acatacggta ttgcaaccat caatgaccgt tccagtggag aatttggacg
     5941 tttgatgaaa caactgatcc gaatagatga tgaaacgaac ttgaataaag aaaacagtga
     6001 gatatgggtc tcacatgctg cactaaataa agcattagcc gcatctccta cattccatac
     6061 tgtttctacc cgtattgacg aattatatac gactcacgcg tggactcaag gtggaaaagt
     6121 aaaagaatta cccggagata tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg
     6181 ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca accaagaatt
     6241 agaagaagcc tatcacaagg gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc
     6301 aggagatgga aagacgatga gtgatctacc aatcgtattc gatttccttc aatcaggata
     6361 cgcttatgca tggcgatata aagatttcca tcaaggagga aattatagtt tcggaacgca
     6421 agaacaagtc aaacaggctc aagaattatt aaaccttccg ctaatggaga attggatcga
     6481 tagtaatgaa ccgagaacac ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt
     6541 catcataaaa atttaccaag ttctggctat tcccgtgctg attgcttcaa tagcagggat
     6601 attacttatg atactaggaa ccgtatttgc aaaagctgat cgtgcctatt atagttcctc
     6661 tttgattgtg gtcaccggat taatactgac acaattggtt tttcttttcg gtgtctcgtg
     6721 gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca ctggcgcagg
     6781 cgtgccgtta acgcaattca tcgtagtaac aagcatgcta gttttcagta aattaaatct
     6841 gaaaaaagct ttatcttcta aataaaaaga cccttggtag ctagattttt aacctaacta
     6901 tcaagggtct ttgtgtggaa aaatcagttt ttattcgatt tcttctacca tttcctgcaa
     6961 acgctgttca tattttttgt ttcctttata taaatcacca atcgtccatg ccaacaggag
     7021 gaggacaaga acaatgatta cataagcgat gtgatcagcc aatgctagtt gagcggaagt
     7081 ggcttgctcg ccgaagaaac tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt
     7141 tagactaatc acagagaacc agccaagaag ttttacgatg aatgaatttt taaatcggct
     7201 acccatctcc gcttggctgt tggtcatcat cagtaaaggg atcatcgaaa acggcaacgc
     7261 aaaagccaag aacacctggg attcattcat taagttattc aaagcggtat gttcttcgat
     7321 cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag aaagcaatcg
     7381 tgtgaccagt cttcttagcc aaatcgggat acgcatatgg atgaatcctt ccatgatgac
     7441 ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa gtgctacagc
     7501 aaataaagtg gacaagatac cagattttgc tacagcaatc aagataccat tgctcatagc
     7561 tgatggatca gataaagctt catacagacc aaaaaaggaa ggatcttgaa cagaaccggt
     7621 tttgaagaca gcgacaccca taatcaatag cagcgcattg actagaaaag ccatcgacaa
     7681 ttggatatta gagtcccaag tagaaaaacg tacagcttgt gctacatctt cttcgtcttg
     7741 gtgatcgatc ttccgagttt gtgaaactgc tgaatgtaga tataagttat gtggcatgac
     7801 tgttgcgcca ataatcccta atgctccagt tagcggagtt tcgccaccaa tgacaggtga
     7861 gtcggagaat gtatggctgt tagggataaa gccagcaaat aaactgtgcc aatctgggtt
     7921 agacaagcca acttgataga caaaaacgaa taaaatgacg aaaatcaagc agacaaccag
     7981 tgcttcgatc ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa catctaatac
     8041 agtaataaag acggcaatca ccaatggaat atcaaaaagt aagtagaggg cgattgctgc
     8101 accaatgact tctgcaatat ctgttgccat aatggctaat tcagtcaaga tccataaaat
     8161 gacacccaat gtcttacttg tgcgtgctcg gattgcttgg gccaaatcca tttgggtcac
     8221 gattcctaat tttgctgcca tatactgtaa aagcatagca atcagactag acatcaaaat
     8281 aactgacatc aataaatatt ggaaattttg tcctccggtg atcgaagtag accagttacc
     8341 cgggtccata tacccaactg ctactaacgc accaggtcca gagtaagcaa gaagtgtttt
     8401 aaaaaaactt ttcccctttg gtacatccac tgttccattt acttcagcta aagataaacc
     8461 atttgtatgt tcaataagtc gatgcttatg aaattgtttg ttggattgat tattcaagaa
     8521 aaaaccacct ttcctattta tctacgatta cattctacac ctcaaaaaga aaaaattaaa
     8581 gaaaaagatt cggtatgcct aatttttttt cgtggcgtgt cttgctagat gacgaaagaa
     8641 attttgttct cctctaaaaa aaccactatt ttttgcatga aactaaagaa tacgcctttc
     8701 ttttttcaat aaactttagt aaaataagtc attgagagca attcggaaat agaaaggaga
     8761 gatttatatg ttatcaacag gtattgtcgt acttattgct attattgctt tgctaatcgg
     8821 cgcagtaggt ggctttttcc tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc
     8881 tcccgttaat gaggacatgt tgcgatcaat gatgatgtca atggggcaaa aaccatctga
     8941 gaagaaaatt cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac
     9001 ccaacgactt tttacgggct gattttttca gtccgttttt tatttcgtaa tttgttagac
     9061 tattcagatt attcagcaaa aagacaagca cagacaatcc taggctcttg ataaagacaa
     9121 aggaggagta aaatgtcgat ttttagaaag ttaggctggt tttttcgtca ggaaaagaaa
     9181 cattatttaa ttggggtttt ctcgctattc gcagtggcct tggtacagtt agttcctcca
     9241 aaagtgattg ggatcatcat tgatgagatt gccgataaaa atttatcagt aaagatcatt
     9301 ttcgggtgga tcttcctatt gattatcgct gcttttttac aatatctttt tcgttatatc
     9361 tggcggacga atatttgggg aagtgcagct cggttggaga aagaacttcg gcagcagtta
     9421 ttcaaacatt ttacagaaat ggacaatctc ttttaccaaa aatatcggac tggggatttg
     9481 atggctcatg caacgaatga tttgaatgcg atccagaatg tagcaggagc tggtatcttg
     9541 acctttgcag attcagtgat tacgggcgga acaacgatca ttgcgatgat tttgtttatt
     9601 gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt cacttcacga
     9661 gtgctggggt cgaaactcca tgatgctttt cgtgattcgc aagaagcttt ttctagtatc
     9721 aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt tggacaagaa
     9781 aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa aaacaaacga
     9841 gtcaattttt tagactcctt atttgatcca ttcattacct tgattatcgg tctgtcttat
     9901 gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc gatcgggcag
     9961 atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc gattggtcga
    10021 ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga acttttgcat
    10081 gaaaaaacgc atatcgtgga acaactgggt gcagtgcaga ccccggcaaa aggcgagctg
    10141 aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt agaacaaatc
    10201 aagtttgcaa tccaagaagg agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa
    10261 acgacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa aatccaattt
    10321 ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat cggctatgtg
    10381 cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt ttctaatcca
    10441 aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa caatgaaatc
    10501 aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc tttatctggc
    10561 ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga attattaata
    10621 ttagatgatg cactttctgc agtagatgcc aaaacagaag aagctatctt gcataattta
    10681 aaagaaataa gaaaagagaa aacaactatc atttcagctc accgtttaag tagcgtgatg
    10741 catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg aacccatcat
    10801 gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca attagaagcg
    10861 aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg acaaaatcga
    10921 ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc aaacctttta
    10981 gaagaacgtt catcgttgct atcttatttg cgtttgctct gtctgtgatc aatgtcctat
    11041 tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg gctacgacac
    11101 aagttatttt gttttttgct ggtatgtact tatttggagt catagtaaaa agtatcattt
    11161 ggtttttcca atggtatttg tattccatgg cttcattgaa gacctatcag tatattaggg
    11221 taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag acaccggcag
    11281 gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt tggtacgtct
    11341 ttttgatggt attgactggg atctttgcag tcgtttcttc attcattgcc atgtttcaaa
    11401 tcaatggaga aattgccttg tggactttgg tgttcttgcc aattttagga atagtcatct
    11461 ggtattacca gaaatttagt tctcgtattt accggaatat gcgggaaaaa ttaagtcagc
    11521 tgaatacgaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag tttcgccaag
    11581 aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac acgcgttatg
    11641 cgatgatcaa aacaaattcg attctgttaa gtccgatcat caattttcta tatgcattgg
    11701 cgattgcgtt ggtgttgact ttgtttggga tcgatgcgtt gcattctccc gtagaagttg
    11761 gactgatcta tgcatttacg acctatgtcc agggattttt taatccaatg tctcaaatga
    11821 tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc ttgaagatta
    11881 tggatacaga agaatatgca ccacaacaaa accctggagc agatcaaatg attacttcag
    11941 gtaagattga gtttcgcaat gtaagctttt cttatgatgg agagcacaat gtattaaaca
    12001 atatctcttt tgttgctaat ccgggagaga ccgttgcatt agtgggacac accggaagtg
    12061 ggaaaagctc gatcatcaat gtgttgatgc ggttttacga attttatgaa ggtcagatat
    12121 tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag aaaatggggc
    12181 tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt cggatgatga
    12241 atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa gcaaacaaat
    12301 tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt gccagctatt
    12361 ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgactgat cctaaaattc
    12421 tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg atccaagaag
    12481 gattggcgaa aatgcgacaa ggacggacaa cgattgcgat tgcccatcgg ctgtcaacga
    12541 ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa cgcgggaatc
    12601 atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg caaagcagtg
    12661 gtatataaaa gatgttgtga cagtagcatt ttgacctaag cactagagtg aaaataaaac
    12721 atagcttccc atatttttca tctgtgagag ttagtgtcgt agttaccaga tttaaaaaag
    12781 acgacgtcag ctagtcagaa gtgcctaata gcccctaaga aagcgttgac aaaatcgttt
    12841 gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa
    12901 acctaatgag tctcccatgg tttcatggac ggctttttgg gtttttcttt ttgttttgga
    12961 tacagcccta tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac
    13021 aacgtcgtaa taacaaggga tgaaagagat cgggaaatga ttgtgatggg aaaaggattg
    13081 gcattcaaaa agaaggtggg tgacccgatt cttgattcat gcatcgacaa aaaatttcag
    13141 ttgacaaatg acggactttc acaacagttc caagaattgg cacaagcgat tcctcttgag
    13201 tacttagaaa tttcttgtga aatcattgaa tatacaaaag aggctctgca agtccattta
    13261 aatgattctg tgtatatttc attgacggat cacttgtata cagctatcga acgtcaaaaa
    13321 atggggatct ttgtgccaaa tgtattgctg tatgatatca aacaactgtt tcctaaagag
    13381 tatgccattg ggaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca
    13441 gaagacgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccag
    13501 caagacaata cctacctgtt taccacaaca atacaggata ttttaaatat cgtccgctat
    13561 tactataaga ttacatttga tgaagattca gtccactttt ttcgattcgt cacacacctg
    13621 cgtttttttc tgacacgtgt tctagatgga gcgaaatcag aagatggaga agtttctgag
    13681 gaattgttcc aagttgttca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata
    13741 gcagactttt taaccggggc tttcgactac cagatgtcca cggatgaaaa aatgtattta
    13801 acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaggaggac
    13861 aaattaacaa gaacgtatga aaataagggt agtgacattc agttggctaa accttccact
    13921 aattaattgg agggaataga aatgggaaaa taccaggaat tagcagaaaa catcgtaaag
    13981 aatatcggag gaaaagaaaa tatccgtagc ttgacacact gtgtgacgcg acttcgtttc
    14041 aaactaaagg aggaaaataa agcaaaggat gatgttttga aaaagatgga agggatagtc
    14101 accatcatga aaagcggcgg gcaatatcaa gttgtaatcg ggaatcatgt gccagatgtc
    14161 tatgcagaag tactgacagt agccgggatt tccgatgaag tagaagaaga ggtttcttca
    14221 agtagtaatc tgttcaatcg attaatcgac atattaagtg gctgtttcca gcctttttta
    14281 ggtgctttag ctgcagcggg aatggtcaaa ggattaaatg ctttgttctt gtttctcaaa
    14341 ttttatactg aaggatcggg tacgtacatc atgctgaatg ggatagggga tgcaattttt
    14401 tatttcatgc cagtgatctt agggtacaca gcagcgaaaa aatttcatct gaatccgatg
    14461 gtggggctcg tgattggtgc agcactttgt tatccgactg tacaaggagc agcactacaa
    14521 agtggtttcc ctgatggcag tcctgcgcct tatagcttgc tgggtcttcc ggcttatgaa
    14581 acatttttgg gtatcccatg ggtgggcagc agttatacca gtagcgtcgt acctgttatc
    14641 ttcattatcg catttgccgg acaagtccaa aaaattgcca agaaatggat tcctgcagtc
    14701 gtccagacct ttttggttcc tttttctgtg ttattaatcg cacttcctgc tggctttttg
    14761 attatcggtc cgattgtcag tatactgaca gatctattaa gcacgggatt ccaagcattg
    14821 atggactttt ctccagctgt gtatggtgca gttctagggt ttctttggca agtactggtg
    14881 attttcggac ttcattggag tattatccct cttgcgatta tgcagctagc gcaaaatggg
    14941 tggagtcaga tgttagtagg cgtttatggc gcgagttttg cacagacagc agtggtattg
    15001 gcgatgtatt tcaagttcaa ggatcaaaaa atgaaggcgc tttgcatgcc agctgtgatt
    15061 tcaggaatct tcggcgtaac agaaccggct atttatggaa tcaccttacc gaagaagaag
    15121 ccatttattt actcgatgat tggtggtgcc atttcaggaa tctatttgat ggcatctgga
    15181 acaatcagct acacaatggg cggattaggg atttttgggg tggtaaacta catcacggaa
    15241 acagacgcca gtggtatgtt tcaagcgttt atcggcattg ctattgcttc ggtaatcggg
    15301 ttttcgctga cttttttctt ttggaaggat gaagcaggtg aaacagatgc tatacagaaa
    15361 aaatcaggta aagaaattgt aaaaacaccg atacaaggac aaatcgctcc actaaatgca
    15421 gcgaaggatg cagcttttgc acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa
    15481 aaaggagaag tacgtgcccc ttttgacgga acagtcatga ccctgttccc gacgaaacat
    15541 gcaattggaa tggtttctga aacagggctg gaattgctga ttcatgtcgg attggatact
    15601 gtgcagttgg aagggaaata ttttgaaagc ttagtgcagc aaggagaaag agtgaaacaa
    15661 ggcgatctgt tgctacgctt cgacaaaaag aaaatcgaaa aggaaggata cagtttagaa
    15721 acacctgtga tcgtcaccaa ttacacggat tatttggata ttttggaaaa ccaaggggag
    15781 acggtcgggc cagacgatgc acttatcacc gcactgacct gaacggttag gtgaagaatg
    15841 gaataggctt aactattctt tttattccac ttctttattt tcaaaattga aaggctcttt
    15901 catgatggct gagctgatag ggcgtttatc gaatttttga ccgacagaaa ttagatggac
    15961 taaattctgt tgataatttt ctgcaaaaac acaactatat aacacgtcta gaagataaca
    16021 aattgatgta ttgatcgtaa aattaccaat ttttgaatac agtcgctcgc gtgtcgtaag
    16081 tcgcaaagcg gctgtactca ttgcagatag cgttgaatca ccgatactgg tcaaagctaa
    16141 aattggaaca tgattttgtt tcaatatttc tgcggtacga agaatcactt tattttcacc
    16201 cgtataagaa atcaggatcc cgcacatatc gctcgtgcaa ttgtacgctt catagaatga
    16261 ttcaccaaaa gtggtgacaa ttgtgacatc cattttgatg cgcttcattt tcagcatgaa
    16321 atcttgagaa atcaaagtat tcgagttact ggcgaagatc acgatttttt ttgcctggag
    16381 cagtaaatac ttggcttttt gcagttggtc atgatgaatg agagaaagtg tatcttgaat
    16441 cgtcatctgt tctaaagaag ccattttatt tgcgatcgtc ataataccgt ctgtggtgga
    16501 aaaaggaaga ttcgcatcta cgtcttgaaa attcccttca agataggctg tttcagctaa
    16561 aaaagcatta cgaaaactga cccagccatc aaaacccatt ttttttgcta cacggatgag
    16621 tgtagacggg tggacatagc atcgctcagc gatctcttgg atggtcattt cgccaagtgc
    16681 tggagaatcg cttaagagaa actggatcac ttcattttct gaaggagaaa aggagatttc
    16741 tttgattttt tcacgtaata acatgtagtt accttcttct tattttttta cgtaaattat
    16801 aacttttttg aaaatgtttt gtaaacggat ttatactata taacttaaaa tagacttttt
    16861 ttgacaagat atttacaaaa atccactaga aaacagtaac ttttgtaaat gttactggaa
    16921 agaaacggat ttctattgtt tgttttgttt acaaaaaaga taatgaaagt gtaaaaaagt
    16981 aaaggagtga gaacatggca aaaggaatca aaatcgctac gattggtgga ggcagcagtt
    17041 acacccctga gttgatggaa ggatttatca aaagatatga ggaattaccg atacgagaaa
    17101 tctggctcgt agatgtagaa gaaggaaaag aaaagcttga gatcgttagt cagatgtctc
    17161 agcgtatgtg ggatgcttct ccgtatgaag tcaaaatcta tgcaactctg gatcgggaag
    17221 aagcattgaa tgatgcggat tttgttacga ctcagtttcg tgtcggtcaa ttagatgcac
    17281 gaatcaaaga cgagcggatc cctagttatt atgggatgat tgggcaagaa acaaatggtg
    17341 ctggcgggat attcaaagct tttcgtaccg ttccagtggt cttggcaatc gttgaagaca
    17401 tgaagcgttt atgtccaaat gcttggctga tcaatttcgc gaacccaagt gggatggtga
    17461 cagaagcagt tatcagatat ggcaaatggg ataaagtgat tggattatgc aatgtgccag
    17521 taatggcgat gatgacagaa ccagcaatgc ttggcaagaa aaaagaagag ctgatctaca
    17581 agtttgccgg tctaaatcat ttccattggc acaaagtagc agataataaa ggaaatgacc
    17641 gcacggcaga attgattgat cgtttatacg atgaagacaa tggattgccg aaaaatatct
    17701 ttgatatccc gtttttcaaa gaacaattaa aacaaatgaa tatgatccct tgtggctacc
    17761 accgctatta ctaccgtgaa gaagaaatgc tggaacatgc tttagaagaa tatcaaacga
    17821 ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga gctatttgaa ttatacaaag
    17881 atccagaatt agaccataaa cctgaacaat tgcaaaaacg aggtggcgct tattattcag
    17941 acgctgcctg tgaaacgatt gcttcaatct atggaaataa agaaacacag atcgtggtat
    18001 ctacaaagaa tgatggagct gttcctgacc tgccggcaga ttgtgtagta gaagtcactg
    18061 cctatgttgg cgcacaagga gcgagaaatg tagcgtttgg ttctcttcct gctgctgaaa
    18121 gaggctggtt gcaagtaatg aagaatatgg aattgctgac gatcgaagca gctgtgactg
    18181 gtgattatgg gacagcttta caggcgttta cgatcaatcc gcttatacga tcaggaaaga
    18241 cagcaaaacg gatcatggat gagttgttta ttgcacacca gcagtattta ccgcaattca
    18301 aggaaacaat cagccgatta aaagaagaag gaatcgaagt tcaagatgaa ctggcaagag
    18361 aattatcagg taatacacat ataatggagg aagtataatg ggattcagaa aagatttttt
    18421 atggggcggt gctacagcag ccaatcaatg tgagggcggt tatgatgaag gtggacgcgg
    18481 actagcaaac gtagatgttg tgccaatcgg aaaagaccgt tttcctgtta tcaccggaaa
    18541 gaaaaaaatg tttgaatttg atgaggatca tttttatcca gcaaaaattg caatcgatat
    18601 gtatcatcgt tataaagaag atattgcttt gtttgcagaa atggggttca agacttatcg
    18661 aatgtccatc gcctggagtc gtatttttcc aatgggggat gaaaaagagc cgaatgaagc
    18721 tggcttgaaa ttttatgagg atgtctttaa agaatgccaa aagcacggaa tcgagccact
    18781 tgtgacgatt acgcattttg attgtccaat gcatttgatc aaagaatacg gtgcgtggcg
    18841 ttccagagaa ctcgttggtt tttatgaaaa tctgtgtcga gtcatcttca atcgatataa
    18901 aggacttgtc aaatactggc tgacattcaa tgagatcaat atgatccttc atgcaccatt
    18961 catgggagca ggactgtact ttgaagaagg agaaaacgaa gaacaagtca aatatcaagc
    19021 ggcacatcat gagttggttg caagtgccat cgcaacgaag attgctcatg aagtagatcc
    19081 ggagaacaaa gtaggctgta tgctggcagc aggagcatat tatccataca cttgcaatcc
    19141 tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat tatttcttca ttgatgttca
    19201 gtcacgagga gaatacccag cttatgcatt gaaagaaatg gaacgaaatg gcattcagat
    19261 cgaaatggaa ccaggggatg ccgcattgct gaaagaacat actgttgatt ttatctcttt
    19321 ttcttattat tcttcccgag tttcgactac cgacaacgaa ctcttggaac agactgcagg
    19381 aaatatcttt gcttctgtca aaaatccata tctgcaagca agtgaatggg ggtggcaaat
    19441 tgatccatta ggcttgagaa taacgatgaa tgatatttat gatcgttatc aaaaaccatt
    19501 atttattgta gaaaacggct taggtgcagt cgatacgcca gatgagaatg gttatgtgga
    19561 agatgattac cgaatcgatt acctggcagc gcatatccag gcgatgaagg atgcagtaga
    19621 actagacgga gtcgatctat taggctatac gacttgggga tgtattgatt tagtatcagc
    19681 tggtaccggt gagatgaaga aaagatacgg attcatttat gtcgatcgtg ataaccaagg
    19741 aaatggcaca ttagcccgtt caaagaaaaa atcattcaac tggtataaaa aagtaattga
    19801 ttcaaatgga gaaaatcttt cgaacgattg atcaatgatt tataaaagtc tattgttttt
    19861 cagcggattt ttcgagtata ataaagctat tcgagaaacg caagcagaac aatagacttt
    19921 ttttcgcatt ggctgtaaag tatgcttact tgattaagta aaggagaatc tacatatgta
    19981 tgatgtaatc gttattggcg caggtcctgc tgggatgact gctgctttat atgcttcacg
    20041 ttcaaactta tccgtattga tgatcgaaca aggtgcccct ggcggccaaa tgaacaatac
    20101 ggcagaaatc gaaaattatc ctggattcga ttcgatcatg ggccccgatc tagcttataa
    20161 gatgtatgaa agtgtctcaa gatttgggac agaaaatgct tatggaatcg ttcaagatat
    20221 caaggatcac ggcagccata aagaagtcat ctgtgaagat aaaagttacg aaggaaaaac
    20281 agtcattatc gcaacaggct gtatccaccg taaattaggc gtacctggcg aggaagaatt
    20341 tgctggacga ggcgtttctt actgtgcagt ctgtgatggt gcgtttttcc gtaacaaacg
    20401 tctagtagtc atcgggggtg gggactcagc agtagaagaa gccatctatt tgacacaatt
    20461 tgcttctgaa gtagtgatcg tccaccgtag agatgaatta cgtgcacaaa agatcatcca
    20521 agaccgtgca tttgcaaatg aaaaaatttc ctttatgtgg gatacagtcg ttgacgaaat
    20581 cgttggaaac gaaatggtag tgacaggtgt gaaagcacgt aatgtcaaaa ccggcaatga
    20641 atttgattta gcagcagacg gagtattcat ctatgttgga ctggatccat taactgcacc
    20701 ttttagagaa gcaggtttga cgaacgaagc tggctggatt ccaacagaca acgaaatgaa
    20761 aacgaatatg ccaggcgttt ttgccatcgg agatgttcgt gaaaaagatt tgcgacaaat
    20821 cactacagct gttggtgacg gcggtattgc tggacaacaa gtgtataaat acttggaatc
    20881 tttagaagaa acaactgcat aagtttaaaa agagtagaag aacgggttca attgttcttt
    20941 tactcttttt tttcgcgtga aatttactga atctgattta tgcgaacacg tccaacacct
    21001 tttgataaaa gaatcctttc ttgatttaag cgttttttgg tcctcatact ttcatatcta
    21061 ataagtgaat gctatactta ttaagaacca tatttcagaa agaggtgtta ttttcatgtc
    21121 ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat ttagaagaaa acttaaaaaa
    21181 acaattaaat gatctgggtg aagatcctga aaaacttgaa gatgcttttt atgcaccgtt
    21241 ggaattcggt actgcaggca tgcggggaat cttaggacct gggatcaatc gaatgaacat
    21301 ctatacggta agacaagcaa cagaaggact agcacgtttc atggatacac aaggtgaaga
    21361 agcaaaacgc cgaggtgtcg ctattgctta tgactcacgc catcagtcac cagaatttgc
    21421 aatggaagca gccaaaacac tggcacatca caatatcccg tcttatgtat tcgaaagtct
    21481 gcgtccaaca ccagagcttt cttttgcagt tcgtcatttg aagacgtttg cgggaatcat
    21541 ggtaactgca tctcacaacc cagcggctta caatggatat aaagtctacg gagaagatgg
    21601 cggacaaatg cctcctgcag acgcagatgc attgacgaaa tatgtccgtg aagtcagcaa
    21661 tccgctaaaa gtcgaagtat tatctgacga agaagctaaa catagcgggt tgatcacgat
    21721 catcggagaa gaagtggacg ctgcttatct agaagaaatc aaagcagtga cgattgatcg
    21781 tgaattggta gaaacaatgg ggaaagattt gaaacttgtg tacacgccac ttcatggaac
    21841 agggaaaatg ttgggcgaac gtgcgttgaa acaagctgga ttcgaacaat tcgtactagt
    21901 tcccgaacaa gctgtggcag atccagattt cactacggtc aaatcgccaa atccagaaga
    21961 acattcagct tttgaatatg ctatacgact tggagaaaaa gaaggagctg acttgctgat
    22021 tgcaacagat cctgatgcag accgtttagg cgcagcggtt cgtttgccag atggttctta
    22081 tgaagtattg actgggaacc aaatcggggc attgtttgct caatatattt tggaagcaca
    22141 tcaatcaaga ggcactttac cagaaaacgc agcggtattg aaatcaatcg tttccagcga
    22201 attgcctaca gcaattgcgg aacactacgg tgctaccatg ttcaatgtgt tgacagggtt
    22261 taaattcatt gctgaaaaaa tccaacaatt cgaagaagac cattcacata cttttatgtt
    22321 tggattcgaa gaaagttatg gttatttagt taaacctttt gttcgcgata aagacgcgat
    22381 ccaagcattg cttttattag cagaagtagc agcttactat aaaaaacaag gcaaaacgtt
    22441 gtatgacggc ttgcaaacga tttttgaaac ctatggctat tttgcagaaa aaacgatttc
    22501 tgtgacaatg agcggacaag aaggttctgc gaaaatctct gctttgatgg aaaaattccg
    22561 taaagaagca ccgacagaat ttgccggagc aaaagttatt caaacagaag actttaaaga
    22621 attgacgaga acagaccgtg aaggaaacgt agaaaaacta acaacaccgc catccgacgt
    22681 attgaaatat acattggaag aaaatggctg gattgctatc cgcccttcag gaactgagcc
    22741 gaaaatcaag ttttatattg gtgtaaaagg aagctctaca gaagacgcag ccgaaaaagt
    22801 agcctcttat gaagcgatta tcaaaaagat gacagaagaa taaaaaactt gagactgatg
    22861 gaaaaagtgg taaactttag aagtcagtct tttttcaagt tggaggtgaa tggattgcca
    22921 gacacgcaaa aatcaaaagt agagaaagtc agtcaattat tcaaaatgtt gagtgatccc
    22981 actcgtctta aaattctgct atatctgaaa gacggggaac aaaatgtgac agcaatcacc
    23041 caagcagtgg aaatggagca gtctgctgta tcgcatcaac tacgtttatt aagagaaaat
    23101 catgtcgtca agtctcatcg cgagggaaaa gcgattttgt atagtcttga tgattctcat
    23161 gtactggata ttttgaacca gacactcaaa catgtggaac atcaataacg ataggtgcaa
    23221 atagataaac actggagtgt gacaacgaaa tttcttcgtt gtcacactcc agtgtttttt
    23281 aagaaaaaga caaaaatgat taatcgcttt tttctgttgt ttcagttcca taataactat
    23341 gatcttctag gtctaaagca ttcaaaataa gcgaagccgt ttcttcaata gataaagatg
    23401 ccacattgat cactacacat cctagttttt cataaagatc attcgcaaaa tcgagttcag
    23461 ctctaatttt ttcgatatcg gaataagcag tatcagggtt taatccgtaa gagcgcatcc
    23521 gttctttacg gatattattt aaaatatctg ggttattcgt tagtcccacg atttttttag
    23581 gatcgatctc ccaaagctgt ttgggaatat gtgcttgtgg gatcaaaggt aaattggcaa
    23641 cttttaaatt tttgttagct aaaaacaagc tcaaaggcgt tttggaagtt cgggatacgc
    23701 ctagcaaaac gacatctgct tctaaaaaac cacgaggatc tttcccatca tcgtatttta
    23761 cagcaaattc cattgctttg atccgtttga aatagttctc attcaataaa tggagggcac
    23821 caggttgccg aattggcttg ataccggtta aacgttcaat ttctgatatc ggtggcgtca
    23881 tcacatcaaa actaaataat tgatgttctt cacaaaaaga acgtgttatc tcgatcagtt
    23941 cttgattgat taatgtatgc aggacaagtg ctttttcttc taatgcatct tgcaatgcct
    24001 gctttaatgt tttttcatct gtcacgaacg ttctgcggaa taggttgaac tcaacggtag
    24061 gatactgagc catagtagct tgcgctagtt ttgtcgcagt ttctccagca gagtcggaga
    24121 tgacaaaaaa agtaacgatt tctgtagaat cggaagacat ggataaaacc tcacttgcat
    24181 tttttttatg tttattatac catactctag aatgcgtact tcgtaatcat tttgatttgg
    24241 aggaataagt catgaaaaaa cagacattga tcgaacaatc attagaaaca ttgaaaaata
    24301 acggttttaa atatactaaa aaaagagaag cattgttaac ttatttagtg aagcgtaacc
    24361 gctacgtatc tgctaaagaa gtttttgact atatgaatca ggaattcaaa ggggtaagtt
    24421 atgatacgat ttatcggaat ctccatgatt ttgcagagat cgatttactg gaagaaaccg
    24481 atttaaatgg agaaatgaag tttcgttttc attgctgtca gggggaaatc ggccatcacc
    24541 atcattttat ctgtacagtg tgcggaacaa cgaaagaaat ccatatgtgt ccgatgaatt
    24601 atttcgaaga acagctaggt ggatgcacga tcgaagggca tagattcgag attttcggaa
    24661 gatgtgaaaa ttgtcaataa gggtttaaat aaaataacat ttgataattt taaaaaaaag
    24721 caagatttcc ttgacggtc
//
LOCUS       DACENJ010000020        24462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.020, whole genome shotgun sequence.
ACCESSION   DACENJ010000020 DACENJ010000000
VERSION     DACENJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.020"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(126..974)
                     /locus_tag="IW748_000608"
     CDS             complement(126..974)
                     /locus_tag="IW748_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1103537.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1198..3138)
                     /gene="thrS"
                     /locus_tag="IW748_000609"
     CDS             complement(1198..3138)
                     /gene="thrS"
                     /locus_tag="IW748_000609"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1103538.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3475..3654)
                     /locus_tag="IW748_000610"
     CDS             complement(3475..3654)
                     /locus_tag="IW748_000610"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1103539.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3701..5524)
                     /gene="cadA"
                     /locus_tag="IW748_000611"
     CDS             complement(3701..5524)
                     /gene="cadA"
                     /locus_tag="IW748_000611"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1103540.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5802..8081
                     /locus_tag="IW748_000612"
     CDS             5802..8081
                     /locus_tag="IW748_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1103541.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8174..8821)
                     /locus_tag="IW748_000613"
     CDS             complement(8174..8821)
                     /locus_tag="IW748_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103542.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9000..9719)
                     /locus_tag="IW748_000614"
     CDS             complement(9000..9719)
                     /locus_tag="IW748_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1103543.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9850..11001)
                     /locus_tag="IW748_000615"
     CDS             complement(9850..11001)
                     /locus_tag="IW748_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1103544.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11218..11958)
                     /locus_tag="IW748_000616"
     CDS             complement(11218..11958)
                     /locus_tag="IW748_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1103545.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11962..12285)
                     /gene="rsfS"
                     /locus_tag="IW748_000617"
     CDS             complement(11962..12285)
                     /gene="rsfS"
                     /locus_tag="IW748_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1103546.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12342..12935)
                     /locus_tag="IW748_000618"
     CDS             complement(12342..12935)
                     /locus_tag="IW748_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1103547.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12919..13566)
                     /locus_tag="IW748_000619"
     CDS             complement(12919..13566)
                     /locus_tag="IW748_000619"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1103548.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13579..13890)
                     /gene="yhbY"
                     /locus_tag="IW748_000620"
     CDS             complement(13579..13890)
                     /gene="yhbY"
                     /locus_tag="IW748_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1103549.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13906..15015)
                     /gene="yqeH"
                     /locus_tag="IW748_000621"
     CDS             complement(13906..15015)
                     /gene="yqeH"
                     /locus_tag="IW748_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1103550.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15012..15539)
                     /locus_tag="IW748_000622"
     CDS             complement(15012..15539)
                     /locus_tag="IW748_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1103551.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15719..16504)
                     /locus_tag="IW748_000623"
     CDS             complement(15719..16504)
                     /locus_tag="IW748_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1103552.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16497..17366)
                     /locus_tag="IW748_000624"
     CDS             complement(16497..17366)
                     /locus_tag="IW748_000624"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1103553.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17367..18746)
                     /locus_tag="IW748_000625"
     CDS             complement(17367..18746)
                     /locus_tag="IW748_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1103554.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18754..19173)
                     /gene="fabZ"
                     /locus_tag="IW748_000626"
     CDS             complement(18754..19173)
                     /gene="fabZ"
                     /locus_tag="IW748_000626"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1103555.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19198..19674)
                     /gene="accB"
                     /locus_tag="IW748_000627"
     CDS             complement(19198..19674)
                     /gene="accB"
                     /locus_tag="IW748_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1103556.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19677..20912)
                     /gene="fabF"
                     /locus_tag="IW748_000628"
     CDS             complement(19677..20912)
                     /gene="fabF"
                     /locus_tag="IW748_000628"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1103557.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20931..21668)
                     /gene="fabG"
                     /locus_tag="IW748_000629"
     CDS             complement(20931..21668)
                     /gene="fabG"
                     /locus_tag="IW748_000629"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1103558.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21680..22597)
                     /gene="fabD"
                     /locus_tag="IW748_000630"
     CDS             complement(21680..22597)
                     /gene="fabD"
                     /locus_tag="IW748_000630"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1103559.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22704..22928)
                     /locus_tag="IW748_000631"
     CDS             complement(22704..22928)
                     /locus_tag="IW748_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1103560.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22981..23943)
                     /locus_tag="IW748_000632"
     CDS             complement(22981..23943)
                     /locus_tag="IW748_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1103561.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23953..24393)
                     /locus_tag="IW748_000633"
     CDS             complement(23953..24393)
                     /locus_tag="IW748_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1103562.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6697 a         5437 c         4258 g         8070 t
ORIGIN      
        1 taaaaaataa acttattttt gaaaattatg ttttcttaag tcgtgaaaca ctttgatgga
       61 tcatttattt gtggaaaact acgttttgtt attctaaaaa acttatataa aaaatagtaa
      121 ggtttttatt tcgaatccat aaaatattct tggattgctt ctcgtgtttt tttcactttt
      181 tcgctaagct tttcgttttt agccatatag aaataaaaaa tgatatcgat cgtagcaaat
      241 tgggccaaaa gtgaattagt tgccgcactt cgattcgtga tttcttttgg tctagatgtt
      301 tgtacgagta catcggctat cttgctaagc gggttatttc caaattgcgt caacgcaatg
      361 atttcgatat tcatgctttt cgccagctcg gctaatgcga ctacatctgc acttctacct
      421 gagtttgaaa caagccaaag cactttcttt ttctcattgc tgacaagttg cgggagcaag
      481 acgtggatat ctttttcaaa aatgattggc ttaccgacac gagaccattt ttgcagaata
      541 tcttctacta ctaaagaaga ggcgccaacc ccatataaaa aaacagtctc tgccgattcc
      601 aaacactcta cgactttttg gatcgtttct tcttctagaa tatctactgt ttcttttatc
      661 gctacttgtg cattgttacc aagtttgttt ttgatagagt aaaaggactc atttgcttct
      721 atatcactaa aaccttcatc gattccagtc tgtagttctg ttgaaatcgt gattttgaag
      781 tccgtcagac tttgaaaacc gatttttttg gaaaaacgta caactgtcgg cgcactcaac
      841 ccggaagctt ctgctaattc attagctgtc attgtaggga ccttctcaat attaccgata
      901 atgtagttgg ccaacttttt ttctgctgaa gaataatctt ccatcatact ttcaatacga
      961 ctaattacgc tcataagttc ctccaccctt ttaatgatag tctatgtaag tataccatat
     1021 cagaccctta ttcacggtcg tatactggat aaaaaacaaa aaaacaatcc gcccaacaga
     1081 atatcttatc ccatcaatcc cacagattct attgagcatc tgtgtctttg aggaatacct
     1141 gttatctatt gttcggattg ctttctatct actacttttt tacaggaaat ttacctatta
     1201 gttttctctg ctgtaatttt tgacttctgc ttccatagca tcaataaaga tattcaaatc
     1261 ttctacagct gtttctttac ttccataacg acgtacgttc actgttgcat cttccaattc
     1321 cttgtctcca acaactaatt ggtaagggat cttttgtgtt tgagaggcac gtatcttata
     1381 gcccattttt tcattacgat catcaacttc tacacgcaac cccttcattt gcaaacgttc
     1441 tttgatttca taagcataat cgccatgtgc atcaacagaa acagggatga ttgttgcttg
     1501 tataggtgcc aaccatgttg gaaaagcgcc tttgtatact tctgttaaat aagcgacaaa
     1561 acgttccatt gtagaaacaa ttccgcggtg gataactact ggacgatgcg tattttcccc
     1621 gtcttctcca acatatgtca aatcaaagcg ctctggcaat aagaagtcta attgtatagt
     1681 tgataatgtt tcttctgtac caagagctgt tttgacttga acatctagtt ttggtccgta
     1741 aaaggctgcc tcgccttctg cttcaaagta atctagttct aattcatcca tagcggcttt
     1801 cagcattgct tgggcttttt cccacattgc gtcatcatca aaatatttat ctgtattgtt
     1861 tggatcgcga tagctcaagc ggaaacgata atctgtaata ttgaagtcag catatactgc
     1921 caccatcaat tcaagcgtac gtttaaattc atctttgatt tgatcaggac gaacaaatgt
     1981 atgaccatca ttcaacgtca tttctcgtac acgttgtaaa cctgacaaag cacctgattt
     2041 ttcatagcga tgcatcattc ctaattcagc gatacgaatt ggtaattcac gataagaatg
     2101 gatcgtgttt ttgtagacca tcatgtgatg agggcagttc attgggcgaa gaacaagcat
     2161 ttcgccatct cccatatcca ttggcgggaa catgtcttct tgataatgat cccagtgtcc
     2221 agatgttttg tacaactcaa catctcccat tattggtgta tatacatgtt ggtaacctag
     2281 actaacttct ttgtctacga tataacgttc gatcgtgcgt cggattgttg ccccttttgg
     2341 aagccagaat ggcaaaccag aacctacttc tggggaaacc atgaacaagt caagttcttt
     2401 tcctagttta cgatggtctc tttcttttgc ttcttcacgg agacggatat attctttcaa
     2461 agctttcttg tcaaaaaagg ctgtcccgta tacacgttgc atcatttgat tgtttgagtt
     2521 tccacgccaa taagcacctg ccactgataa taatttaaat acttgtatac ggccagtgga
     2581 tggaacatgt ggtccacgac acaaatcgat gaattcgcct tgttgataag cagtaatcac
     2641 ttcttcttct ggcaattcac taatcagttc tactttgtac ggatcacttg caaaaatttc
     2701 taatgcttcc tgttttgaaa gaacacggcg ttcgatcggc agattttctt tgacgatttt
     2761 catcatttct gcttcgattg ccggcaaatc ttctgcagtt acttggtttt gtccattatc
     2821 tgtatcgtag taaaaccctg aatcgatagc tggtccaacg ccaaagtgga tatttggata
     2881 gaggcgacgc atggcttggg ccatcaaatg agctgctgaa tgacggacta agccaagtgc
     2941 atcttcatgg tctggtgtga tgatctcaat acttgcatct tcctcgattg gacgagtcaa
     3001 atcaacgagt tttccgttca ctttaccagc taaagctttt tttgctaagc tttttgaaat
     3061 gctttcagca attgccaatg ttgttgttcc agactcaaat tctttgactg caccatctgg
     3121 aaaagtaatt tttatcataa tagtttcctc cttgaaatgt tttgattgat cagatcatca
     3181 agctgaaaaa caaaaaaatc ctagcatctc ttgaaaagag atactaggac gaaaagttcg
     3241 tggttccacc taatgttcaa acgcaaatgc gttcctcaag cgttgtaacg gtccgaccgc
     3301 ccttgcttca acaagggttt cgaaagtggt catcctttgg ttttctcctg gaaaagtttc
     3361 agcctagctt ttcctctctt atcgaagact ctcaaattca gttggctttc tcatcaatca
     3421 attatgtgtt tatttaacca ctaataaata gaaaaatcaa gtgggaaact aaacttattt
     3481 tttccattcc ccatttacac cgtatgttcc ttttttcatc tcattgatga tgacatggat
     3541 gtgttctttt ggtgcctgcg tgtttttatg aacagcttcc gtgatatctt tcatcatatt
     3601 ttctaattgt tccggactgc gaccttctac taattcgaca tgtacgaatg gcataatgct
     3661 ctctcctctt tctgatcgtt cttttttcaa ctcgattcta tcatactatg tttgtcttgg
     3721 gtactgaatc aggaaaaatg gctggatctt tttttgctct caaggcactc aaaatagaga
     3781 ccgtatccac gacttcttgc aaaacagctc cgaataatgc ggggattagt cccgtacttg
     3841 caattaacat caaaaaaata caaataaaga tgccaatcag tacagactgt ttcgctattc
     3901 tcatcgtatc tcttgaaatc actactgctt ttgctacacg caagagatca tctttcaaga
     3961 tgaccgcatc tgcactttca ctagcagctg tagaaccatg agcccccatt gcaataccga
     4021 tatctgcagc agcaagtgct ggtgcgtcat tcacgccgtc tcctaccatc gcaacaggtc
     4081 tttgatcttt agggatactt tctaaaatgg tcagtttatc ttgaggcaag ctacgggcat
     4141 gtacagaatc taattggact ttttctgcaa tcgtttgagc aatggcagat ccatccccgg
     4201 tgatcatcat caaatttttc acatcaagtt ttcttagctc cttgattgtc tttgctgctt
     4261 ctgggcgaat ctgatccgaa aataggatcg ttcctgcaaa agatcgatca attgttacat
     4321 agattgctgt attggacgtt tctatttctt cagctgaaac aaaagaggca cgtccaatct
     4381 ttacttcatg attattgact actgcttgga ttccttgacc ggtcacttct ttcagttgac
     4441 taacaggata cagttggctg tttttcacgt tgttcactaa cgaacgagct aagatatggc
     4501 tagattcctg ttctgcacta gctgcataca gaagtagctc ttcttttttt attccattga
     4561 ctggctgaat cgcctctact tccaatatcc ctttggtaag cgttcctgtt ttatcaaaag
     4621 caatcgtttt tgtacgtgcg agcttttcaa tcgttgttcc agtttttaca acgatcccgt
     4681 ttttacttga acggctcatt cctgcaacta aagcaatggg cgcagctaga atcagtggac
     4741 aaggtgatgc cactaccagt acctcagcaa atcgtaaagg gtcttttgag aaccaccaag
     4801 aaataccacc gatcaaataa gcaatcaacg tgaaagggac tgcataacga tcagctaaac
     4861 ggacaaatcg ggcaggcttt tcttttgatt cttcgactag tttaataatc atttgatact
     4921 ggctgtctgc ggcacgtttt tcagcgatca tttggatcga tccgtctcca tttaaagacc
     4981 ctgacataat ctgatctcca ggtttcttgg tttccgggcg ggactcacct gtcaaagaag
     5041 attcatcgac cgaagattct cctttggtca ctcgaccatc tgcgggtaca agttcgccag
     5101 gcttgactag taactggtca tcaatattta tttcatcgat tactatatcc tgcaattttc
     5161 cttctttgaa tcgatgggca attcttgggg tgttatctaa caaagactgc agttctttac
     5221 ccgctcgttt attcgcgtaa tcctctagac tatctccgcc cgtaagcatg atcaatatca
     5281 tcaaactagc ccagtattct ttgacggcta atgttgcaag gattgcggta atcgctaata
     5341 catctacgcc atatcttcct gaacgtaacg tcttgatcat ttccaaaaac atcaagaagg
     5401 cgagaatccc tccagtaaat gcgacaatcg caaatgcaag ggccggctgg tgaaaaacaa
     5461 attcacttaa taaagctatc ccaccaataa gcaaagtaag taaaaatttt ttgaaatgtg
     5521 tcattgtttt ttctcctctt ttatgtgact tagagaggtt agtaataaat ttccagctgc
     5581 ctctttcatc ttaagcatag tccttgacta gcacttttta aaacaatagg ccttaattga
     5641 aaacaactat cactatcagc gtttccaaaa ttcatcagtt ctacaaacaa aaatattcag
     5701 caaggattac tttttcgatt catcagttaa aaagtacctt ttttcgaaaa actatgctat
     5761 acttttaatt cgttatttac ggcggaagga gcaaacatgc gatgaatgag aaacaactag
     5821 aacaaacgca cgtggatgaa acgatcgaac taatccagat cgaacaaaat atgctggata
     5881 caaaacaaca agaattgact caggaaatga atcattcgac aaaagacaca gctaatcaca
     5941 agattcgagc tggttccaat gaagcttttt atgaatctgc tgttgaatat cgccaacatg
     6001 agcaggaatt gcttttgaaa taccatactc ttgaagcaca gcaaaaacgc ctgcaaacgt
     6061 tgtctgtcat gaaagatagt ccttattttg cccgtatcga ctttaaagaa gaccgtgaaa
     6121 cagaaacatt atatctaggt atcgcttctt taagagatct ggcagaagaa acaatcgtta
     6181 ttgactggcg cgcaccaatt gccaatcttt attatgaagg tgagattggt cccgcatttt
     6241 atgaaactga aacagataga atagatgtcg atctgcagtt gaaacgacaa tttaaaatcc
     6301 aagaaggacg tattttatct atggtagata cgtcggaagt aatcaatgat gactttttgc
     6361 tagagatatt ggatgaatca tcaagcaacc atatgaaaaa catcgtctct acgatacaaa
     6421 aagcacaaaa tgcaatcatt cgggacacaa caagtaaagt aatgctgatt gaaggaatcg
     6481 ccggaagtgg aaagacatcc gctttgcttc aacgtattgc ttttctgttg tatcataatc
     6541 gcaagtggct cgaggctgaa aacgttcttc tattttcacc caaccatttg ttttctgatt
     6601 acatctcgac tgtattgcct tcattaggtg aaagcggcgt gcctacacag acattcaaaa
     6661 attatttggt gcagctgctg ccagagtttt ctttactaga tgaacagcaa caagaaagtg
     6721 gttttttgct aggggaaaaa gatccgatac aagtgatgaa aagcggattg accttagtcg
     6781 atcagattga tcgctatatc caaggcatca caaactacgg tcccttgttc cgtgatatga
     6841 aaataaatgg ccgtacgatc ctatcaaagg aatcgattcg ccaatggtat aaagaaacaa
     6901 atgaacaatt acctttacat catagacttt ctctattaca aacgaagctc ctgaaaaaat
     6961 taggcggtct gcaaaaagac gaaacacgtc agcaatgggt caaagatctg gcggaagaac
     7021 agctccaaga actatacgca acagatccaa atcttgaata tacagaacaa aaggagcgtt
     7081 cattacgtaa aaaactggcc aaccaaatcg tacgtaaaag attccgtcga atccatcgag
     7141 ggatcatgaa ttatcaattt gtcaatataa cgaaacaata tctccacttt ctacaaactg
     7201 tgccactttc tcttctaaca cgacataaaa tcagcgagac caggtggcag gaacatttga
     7261 caactgttcg tacacacttg cgtcaaagag aattgcgtca agaggatgct gtactattct
     7321 ttttactgat gcgccgaaca tatccagtga cggttacaca caaagctcgc tatatcttta
     7381 ttgatgaaat gcaggatttc gcaccatcac aagtggcatt gctgcaagcc ttgttcccaa
     7441 aagcaaattt gactttctgt ggagatctaa atcaaaatgt ctttggaaat gaaacgatcg
     7501 ttggatcatt ggaagacctt ttcgtggacc gtgaagtcac tcgtttccaa ttaacgacaa
     7561 gctatcgctc cacaaaagaa atcactgatt ttgctaatca ttttattgtg aaagaacatc
     7621 aagtagaaac aacagcaaga aaaggagacc ttcctttgct catccaagga acaacagatg
     7681 atatcaaatt gaattggtta gcagatacca ttcgaacgac tcagggaaaa gcccggtatt
     7741 ggcgtacagc aatcatagct aaaacacaag aagaatgcga agctctttat gctcaactgc
     7801 cagacgagtt gaaacaacaa gtccagctga ttttaaatga gagtgacttt atgaagcgtt
     7861 ccattatttt acttccagcc tttttagcaa aaggtctgga gtttgaccga gttttcttgt
     7921 gggatgtaga tgcagaacat ttttcttctc agcaagacaa attgatcctt tacaccatgt
     7981 gtacacgtgc gatgcatgaa ttgacccttt ttacctccaa taatgaaagt cctttgttaa
     8041 acaatatgga tatttcatct tatgagaaaa aagtactgta agtaaaaaga caactgctat
     8101 cattaatgtt agcagttgtc tttttattta caataataat tgcagaaaaa gtaattcaat
     8161 catttaaggt catttattct atatataaat ccaaatctgt ttcaggatat tttaatttga
     8221 caagcgaaat aatcattttc agttcttcca tgttttctgg atattctggg tcaaatacct
     8281 tgctatgacg gccaatcccg ccattgtcga tcaatcggta aattcgattc cattcaatga
     8341 attggtcaat tgattggaaa ttgctgattg gatagccgcc acaatccaaa atctctgata
     8401 cacgaaatgc taggtatgca ctatcgatca ctttattttt tttcgtgtct atcttctcat
     8461 ttctttcgat aagaattttt tcaggacgat gtttcaagct ccactctaat agtagaaaga
     8521 aagcctccat ccgatactct tcttcaaata accgaaaggc tgaatcagaa cgacgaaaaa
     8581 gcagcggaat cagataattt tgtctataaa tcagcattgg tgcgcggtcc accatacact
     8641 ctaaataacg gttcagatac cctgtaccga catagtttcc taatcgatag ccttcaacgc
     8701 cgaatcttgc gtatttttgc gctaattttg aacaatcagt tacgttcaca gctaactctt
     8761 ctactttgcg gtaaccgctt aagatacgtt caaaaggttc taaagtgatt tttgcttcca
     8821 tattcttccc cgtccatctt ccacatactt catatcattt cacatgatac ttatgctctc
     8881 atcataccac aggttgaatt agaaaagaaa ataaatcaca caaaaaagca agacaatctg
     8941 ttagtgagat tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa actggttgct
     9001 tagtcttcta atgccacatt atggaacaca tgctgtacat cttctagatc ttccagtgca
     9061 tcaatcattt tttcaaatgt ttcttgatct tctggagata gtgttacctc gttttgaggg
     9121 atcatttgga tctcagcgat tgaaaaatca gtgattcctt tttcttttaa tgcttcttgt
     9181 atactatgga aatccgctac ttcaccgtaa acgatgattt gtccttcttc ttccattaca
     9241 tcgcgaacat cgatgtcttt ttccattagg tattcaagga tctcgtcagc gtcttctcct
     9301 gcaaaagcaa agattgctgt attgtcaaac atgtaagaaa ccgctccgga aacacccatg
     9361 ttcccgccat ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt cacattgttc
     9421 gtcaatgtat caacaatcac cattgagccg cttggtccaa atccttcata gcgcaattca
     9481 gagtatgttt catctcctgc accttttgct ttttcgattg ctcggtcaat aatatgtttt
     9541 ggtacattat aagttttagc gcgatcaatg acgaaacgta gtttttggtt tgcatgcgga
     9601 tcaggatcac ctgattttgc tgccgcgtag atttcaatcc caaattttgc ataaatccgg
     9661 ctattattag catccttagc agttttcttt gctacgatat ttgcccattt acgtcccata
     9721 ttttcacttc gctttctgat ttgatagttt cataacgcaa aaacgtcctg ttccattata
     9781 cttgtgaatc acgattctgt gaagcttttt ttctttcttc cttcattctt ctcattttta
     9841 gtcaattatc tatactcgga ttgggatttg cccgaaattc tgttccggaa tattgcctcc
     9901 caattgatag atttgatcac ttctaactaa cagttccgct tcttgtgcct ctttttttcc
     9961 gattcgggaa taaatagaaa tctctttttt ttcttttaag aatcttcttc cagcatcggt
    10021 aaatccaagt agtcgaagat aatttttatt ttgttggaca ggaatctctt gctttttgat
    10081 attcagcaag acataacaag ataatcgttg gatccgtgtc catgtatatc tttttgtttt
    10141 tacctttgac acaaaatcct caaaactatc agcagttgca gctgctgttt tcatgcgcga
    10201 ttctattcct tcggtcatct gatagatatc tgtcagttcc agcaaagtag aagataacaa
    10261 ccgataacgc aaatacggcc aataagattc ccaagtctgc agcgaacaag ttgaaagatc
    10321 ttcttctgtc tgaggcggca caagtccagc tattcttttt ccttcttcta acgcatggcg
    10381 aatggctgtt gcactggcta tcctctgatc tgtgatgatt ttttcatgat agcctgcact
    10441 tttacgtcgg atcggatgta attccatagg gtggacatac ttggcatttt cctttgcata
    10501 acttaatccc aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg gatacagact
    10561 gcgaaaaacc tcagtcattt tttgcggata actatacgtt tgatcagaca gttcttgaaa
    10621 tgcctcttct atcctatctt gattctcttg gacaaagcgt ccgaaactgc tgtaatcaaa
    10681 cgaagtctct tcttctgttc caaaacacaa actgtcacag tccagtgttt gcaagatttt
    10741 tactgcacct ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg gaagctccac
    10801 tactaaatcc acaccatttt gtaaagcttc attcgcgcga tgccatttat cgatgatagc
    10861 cggttcgcct ctttgcaaaa aattcccgct cattacagca atgacgacct ctgctcctgt
    10921 tatttttcgt gccatttcca catgatagcg atgtccattg tgaaaaggat tgtactctac
    10981 gataatcccg cagcttttca tgcgcatcct ccttcatagt tatcgataaa aaagaaatag
    11041 agacaagtaa aaaaaggcaa aaatcggaaa aaatacccga actatataaa ctattctcgc
    11101 aaacacaata cttctgcgac gaaaagctgc actgcaagag caatgcctat atcgttcgga
    11161 tatataattt atgttgaaga tttattcttt tcttgttaac tcgagcaggc aaaaccgcta
    11221 gtctttgtat gctacaaaga accagcgctt tgatttctct gatgctgctt cgtctgtaaa
    11281 atcagaaaat gcttcgactt tcgaaaagcc gctgttttcc agcattctta aataactttc
    11341 caatggatag gtacgctctt gatgtagttc atcttcacga ataaatcgac cagacccttc
    11401 ttgttcttca acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc ttcctggata
    11461 actatcccac aaaaaagcaa aattatcccc ttgataatga tagctatatt ctggaaaaac
    11521 ctcatctact tgataggtgg agtgtacgtc aaataaaaat cggccatttt cttctaacgc
    11581 ttgatagact tcatcaaaca cttgctgtac ctcttgtcta tttttcatat agcacagcga
    11641 atcagaaaaa caggtaattg cctgatattg tccgatttct gataaatcga gcatattgcc
    11701 ttccacaaat tggatcgatg cgtcttcttc atatgctcgc tggctggcaa tcatcaacat
    11761 ctcttctgaa aggtctaacc ctgtcacttt atatccatct tttgcaaagg caaccgctaa
    11821 tgctccagta ccgcatgcca gttccaaaat attttttgtg tcttcttgta aatgacgttt
    11881 cgaaaaagct aaccattgct gataaagtgt ttcgtccatc acttcatcgt aaatgaaagc
    11941 aaatgtttca taagccatat gttaatccaa ccattcacta agatcgacta gcggagcatc
    12001 tgaccatagt ttttccagat tatagaaatt tcgttctggc gcatggaaaa catgtacgat
    12061 cacatctcca agatcgatca atacccattt tccgccttct tttccttcaa tacgttttac
    12121 ttcatatttg ttttcttctt ctttatcaat gatttcttct gtaattgcgt tgatttgacg
    12181 ttcgctattt gctgaacaga tcatgaaata atctgccaac aaggaaacat ttcgaacgtc
    12241 taaagcgata atgtcttctg cccgttttga atctgctgct tcaatcgcaa tttttaattg
    12301 ttctttacta tctatgatga tttcctccta atatgagcag cttatttttt tgctacccag
    12361 taattatatg tttcgattgt ttttggataa atctgttgtt cttgcgcaag caaatgggcc
    12421 aatgtatgtt ttgtttcaaa tgctacagct tcgtctagat cagcccatgc aatggctcgt
    12481 gcatcttgta ccccaggaaa atctcgacct ggttcaatat agtctgctac ataaatgatt
    12541 ttgtcaagga gactcatctt agcagcccca gtcgtatgca cccggattgc atgaagaatc
    12601 tctgcatcgg tgattcctaa ttcccgctct acaaacgaag cgccaacaag accatgccag
    12661 attgcattat tataattcaa taactccgga tcaaaaccgt ctcttacgat taccattttg
    12721 aactcctcat ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat actagctttt
    12781 tcaggagaag caccgtattt tttggcaaga gccacggctg tttcttctac tccaagcaca
    12841 tgcttgaagc gttgttcgct cattctcatc tgtatttttt gcatcaggtt ttctctagaa
    12901 taagcagtat attcattact atagtccata ttcatacaac ccttcatttt gaatataatc
    12961 aattacttta tctggaacaa ggtagcggat agaacagcct tctttgatct tttgacgtat
    13021 tttcgtggaa ctgatgtcga tctcaggtac atctacccaa attacaggat atggagtatc
    13081 cgttgtgtat cctggtcgac gtatacccac aaagttgacc atagaagtca gctcatcgat
    13141 tttataccat tttggcaaat attctaccat atccccaccg ataataaagt aataatctgt
    13201 atccggattg ttttgcgtca attccttcat agtatcatag gtgtaacttt tcccaccacg
    13261 agctaattca atcgtttcaa tctgcaaaaa aggattgtct tccaccgcta attccaacat
    13321 gttgagtcga tgttttgcat caatcgtctg tttctcatca acatgaggtg gctgataaga
    13381 aggcatcaaa aatacgcggt caagacctaa attccggcca gcttgttcag ccatcactaa
    13441 atgtgcaagg tgaacgggat tgaatgttcc gcccaagatc cctacttgct ttttcttctc
    13501 taagaattga ggcatttctt caggaaataa ttgtgaacga acaaatgtct tagcttgcgt
    13561 attcatcatg gtcactcctt atattgcttt tacttctttt gatagccttt gatacttttc
    13621 ttttgtggaa ggtttgtata atacaataac acgtccaatg atttggacga tttcgcaatc
    13681 tatttctttt tctaatactt gagctgcttc ttctgcaatc tcatctgtat tttgaagtaa
    13741 cgatactttg atcagttctc ttttttctaa agcttcttca atttgaacga tcattgctga
    13801 attgatccca ccttttccga tttgaaaaat cggctgaaga tgatgcgctt tgctacgtaa
    13861 aaaacgcttt tgttttcctc ttaaactcat aattttcctt cttccttaaa tcaatgcttt
    13921 gcgtttcaat acatctacac cctttggcgc ccagcccgca attactactg gcgctgttac
    13981 agtgatccag cctagaccgg caaaaacgat gtctgttttt tctttcacag aaaactcaaa
    14041 acgaacaagc tccggaaatg cctcgacttc atcaggacga ggaggctgta ataatccacc
    14101 tacatgtttt tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa tatttacatc
    14161 atttgacaca taagcaataa atgaagcacg attgccgctg ataaaatcaa atcttgcaag
    14221 tccgcccaag aaaagtgttt gcccttcatt cagctgataa actttaggtt tgatttcttt
    14281 taacggtgca atgatccgca aatctttttt acctaagtag tgcgccattt gatggcgatg
    14341 gataattccc ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct cgattttatc
    14401 caaggtcgtt cctggaaact gcgaagttgt gattacatct tgtactcccg ctgtattttt
    14461 gatgatttga ttgataagcg tcgattttcc gacatttgtt actccgacga tatagacatc
    14521 ccgtccatcg cggtattctt cgattttttc taatagttcc gtcatctcat gagatttctt
    14581 agcactagtt aataatacat ctactggacg aattcctgct tcgtaagcac gttccttcat
    14641 ccattgtgtt aatttagttt ttttcaatga tttaggtaat atatccactt tgttcccaac
    14701 taaaagtact ggattgtcac cgacaaaacg gtgtagtcct gggattagtg aaccattgaa
    14761 gtcaaaaata tccaccacat tgacgatcaa tgcatctttt tgccctaatt cattcagtaa
    14821 gcgcaaaaag tcgtcatccg tcaatgagac atcttgggtc tcattataat ggcgtaatct
    14881 gaaacaacgc tgacaataga cttccccgga agctaaacct ttttccaaag ccgtttgcgg
    14941 cgtgtatcct aattcaccgg gtttgtttgt ttgtataacg gcaccacagc cgatacattg
    15001 taattcttct gtcattcgat tccacctcgc cagcccatat cagggtgttt tgctaataga
    15061 taggccataa ttcttttttc ccaaaaacga ttaaattgtg tcttccaact atctgttgcg
    15121 ataatgggtt ttacaaggat gctacgtatc cctgcacgat tggctccacg tatatcagtc
    15181 ataatctgat cgcctaccat tactatctca tcatctgcta aatccaattg tttctttgct
    15241 atgttgatcc ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag atcaaatttt
    15301 tcaatcgctc gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac agggattcct
    15361 gcatttttca tttctaaaat ccaagtaagc aactcttctg ttccatctgg attattccat
    15421 gcaatcaacg tattgtctaa atcggtcaat actgctttga tgcctagatt ctttaattgg
    15481 gcaggtgtaa tctgatagat ggcttctacc atccatgttg gtttgtaatt tgaaaacatg
    15541 ctgtctcctc atctgcaaaa aatgaaggag cacataaagt actcctcaac cccgtattat
    15601 actgcatatc agcaaaaaaa actacctcta tactcgttcg tctttcagaa aaagcatttc
    15661 tttacaaaat gaccaaaaat aaagattgat cacagaaaaa tttagaaaca aaaaacaact
    15721 aatattggcg gaacctcttg tatcgttttt ccagtaattt atgctgatct aatcgaacaa
    15781 gttccgtcaa tctttcaatc aacgcttttt tcataatgtg tgcgacttta tatcgtggca
    15841 actgctcgcc gttgaacgtt tcaggaataa ctcgatcaac gattcctaac tcgcgtagtt
    15901 ctggcgcagt aattttcatc aactcagctg cttctgcggc gcgactaccg tctttccaca
    15961 aaatcgacgc aaatccttct ggagaaagca cagcataaat cgtttcttcc agcatccaga
    16021 cttcatccgc taaacctaga gcaagggcac cgccacttcc accttcacca atgatgatgg
    16081 cgatgatcgg tacctttaaa tcagacattt cgagcagatt tctggcaata gcctctcctt
    16141 cgccgcgctc ttctgctcct acaccgcaat atgcacctgc tgtattgatc aaggtaatca
    16201 ccggtctatt gaacttttcc gcttgtttca tcagtcgtaa agcttttcga tagccttcag
    16261 gatggggaga accaaaattt ctgcggatat tttcctgcag atcccgtcct ttttgaatac
    16321 cgatcactgt taccggtttg ccatctaatc gagcgattcc accaacaatc gcttcgtcat
    16381 ctgcaaataa acgatcacca tggcattcct cgaaagaatc gaagatctct tcaaaaaaat
    16441 ccaagcttgt ccatcgttct ttagctcttg ctaactgaac gacatcatgt gctgtcttat
    16501 ccacgatcta tccatccttt ctgtgtatgc aattcaagaa gcgtatggat tttctgtttc
    16561 aattccatgc ggggaacaat ctgatcgaca aaaccatgag ataataagaa ttcagccttt
    16621 tggaaatctt ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct tccagcaaaa
    16681 ccgatcaagc tttggggttc tgctagaata atatcgcctt ccattgcaaa actggcagtt
    16741 actcctccgg tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc attgctatgc
    16801 cgtttgacag cagcagagat ttttgccatc tgcattagag aaaagattcc ttcttgcatc
    16861 cgtgcgccac ctgaagctgt gaacaagacg actggaagac tttcctttgt tgctcgttcg
    16921 aataatctgg tgatcttttc tccgacaatc gttcccatac ttcccatgat aaaattagga
    16981 tccatcactc caataatgaa gggaatcccc tgaatggttg ctttaccagt gaggactgct
    17041 tcatgaagtc ctgttttatc ttgcattttt ttaatttttt ccgtatattc gggaaatgct
    17101 agcggatctt tggttacaag atccgtatcc cattcttcaa aacttttttc atcaataatc
    17161 aaagctagac gctgccatgc accgattcga aaagcgtatc cgcaatgagg gcatactttt
    17221 tcttcaccga tgtctttcgt ataaaggata tgtttgcaat ttggacactt tgcccacatg
    17281 ttatcaggaa cagatggcga gttagcaggt ttgctctctc tattaggatt gatccgaata
    17341 tatttctttt tcttaaacag agccatctat gcgcctccta tctccgtttc tggtgtccaa
    17401 ttcggtaaaa attcttcttg taaaaatgct gtgctgtaat cacctgctat gactgcagga
    17461 tgactaatca aatccatctg aaattctgcg ttcgtgatga ttccttctgt gataaattca
    17521 cttaaggctc gctgcatctt cattaatgct tcaaaacgcg tatttccatg gacgattatt
    17581 tttgcaatca ttgaatcata atatggcggg atcgtatatc ccgggtaaac tgcactgtct
    17641 actcgcagtc ccatcccacc tgctggaagc agtaagtttt ggatcttccc tggagaagga
    17701 gcaaaatgga aagcaggatt ttctgcatta atccggcatt cgattgcgtg tccttggaat
    17761 actacttctt cttgacacac attcaaaggc tcaccggctg ctatctccac ctgttttttt
    17821 actaaatcga ttcctgtgac catttctgtt actggatgtt ccacttggat tcgagtattc
    17881 atttccatga aataaaaatc gccttcttca tccatcagaa actcgatcgt tccggcattt
    17941 tcataattga ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc tctttttgtt
    18001 tgtgagatca ctacagaagg agattcttcc agtacttttt gattgttgcg ttgtaaagaa
    18061 caatctcgct cgcctaaatg aatgacatga ccaaatcgat cacctaagat ctgaacttct
    18121 atatggcgag caggataaat gattttttcc aaatacatat catcatttcc aaatgccgct
    18181 gctgcttctt gctgagctga agaaaaatgc tgtgggagct cttcttttga ttgaactttc
    18241 cgaatgcctt tcccgccacc acctgcagct gctttcagca tcactggata accaattctg
    18301 tctgctattt ccaatgcttc ttctactgta gataatacac cagtacttcc tggaatgact
    18361 gggacatttg cttcttgcat caattgacgc gcattgatct tatttcccat tgcatcgatc
    18421 gttgcacttt taggaccaat aaatgtgata ttgcactctt cacacatgga ggcaaatcgg
    18481 ctattttcag ataaaaaacc aaatcctgga tgaattgctt cggcttttgt cacgattgca
    18541 gcgctcagta cttcctgaac attcagatat gaatcaacgg cttttgccgg gccaatgcag
    18601 attgcttcat ctgctaactg tgtatgaagt gcttcggcat ctgcttctga atatatcgct
    18661 acggtttgga tccctagttc gcggcatgca cgaatgatac gtacagcgat ttcaccgcgg
    18721 ttagctatca atactttaga aaacatattt cacctatcca atcataaagg ttaattccgc
    18781 ttcggctact tttttaccgt ctactttagc gattcctttg cctattccgg ctgaagcttt
    18841 cactttcaat atctcaactt caaggtataa ggtatctccc ggtgtaactt tcttacggaa
    18901 ttttgctttg tccaatccgc cgaaataagc cgtctttccc ttgaattgtt caagagaaag
    18961 taatgcaact gctcctgctt gagccattgc ttcaacaatc aatacccctg gcataacagg
    19021 ttcttctgga aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt tcttagcaac
    19081 gatccgttct ccttcaacca tttcttccac tcgatcaatc aaaagcattg gataacgatg
    19141 tggaatgatt tcttttattt cttgtatgtt cattgttttc cctctttcct gctttgctta
    19201 tttttgctga atcctaaaga gtggctgtcc atattcgaca actgactcgt tttctacaag
    19261 gatttctgtg atcactccgt cggcctcact agttatctca ttcatcagtt tcatcgcttc
    19321 aataatgcag ataacttctc ctttggcaac ttgatcacct acttttttga aagctggttt
    19381 gtcgggcgct ggttgcagat aaacgattcc cacgattggc gaaacaacct cgattccatc
    19441 aaatactgcc gattcttttt ctttcattat ttctggagca gttgttgctt gttcaggtat
    19501 gacattgact ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat tcatgcctcg
    19561 acttgtttta tttttattca tatacaattc aaattgacct tcacgcaaat cgaattccgt
    19621 tagcgaggat tgatcaaatt gactgactaa ttctctgatt tcattgatat ccaaaattat
    19681 tcctcccagc gtttcatgca cagaaccgca ttatgtccgc caaaccctaa tgaattactc
    19741 attgcataag taaaacttgc aggtttgctt tcgtttttga caactgtcaa gtcaatctca
    19801 ggatcttgtt tttctacatt gatcgtaggt gggataaatt gatgttctaa tgccagcaaa
    19861 gttgccaagg cttcgatccc gccggctgct cccaataaat gtccagtcat cgatttcgtg
    19921 ctgctgacgt aagtatctct gaaattttca cctaatgcga tttggattgc ttttgcttct
    19981 gcctggtcat tggctggtgt acttgttccg tgagcattga tatatccgac ttgttccggc
    20041 tgtacaccag cttcttccat cgcaagcttc attgctttag atgcaccaga accatctgga
    20101 gtaggagctg tcatatggta agcgtcacag tttgcaccgt atccaacgat ttctccatag
    20161 attttggcac cgcgagcttg tgcatgttct aaagattcaa gaactagaac gcctgctcct
    20221 tcacccatca caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg attaggatca
    20281 gttgattctg agagtgctgt taaagctgca aatccagcaa tccccatctc acaaatcgtt
    20341 gcttctgttc ctccagcaag aatgacatca gaatagccat gtttgatgtt acggaatgct
    20401 tccccaattg aattcgttcc gctagcacaa gctgttacga tagctgtaca aatacctttt
    20461 gcacctacac gcaaagcaat atttccagct gccatgttgg caatcgccat tggtacgaac
    20521 attggagaaa ctcgttttgc acctttttca tgtagacgaa taacttggtt ttcgattgtt
    20581 ggaagaccac cgatcccaga tccgatgatg acgccaaacc ggtcttgatt cattttttct
    20641 ttatctaaac cgctcatctc cattgcttct aaagcagcgt agattccata gatcgaaaac
    20701 atatccattc gtttgctatc ttttttaata aaatatttat cgtaagggaa atctttgact
    20761 tcacccgcta aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg tgcgatgcca
    20821 ttcgtcccct cttttaagct attcaaaaag ctgtctgcgt catttccaat aggtgaagtg
    20881 actccatagc cagtaattac cactcgattc atttgtttgc tctccttttc ttatccgtgc
    20941 atgaccaatc cgccatcgac attgatgact tgtccggtga tatatggatt ttgtgctaag
    21001 aaaactgctg tttgtgcaat atcttctact tgtccgaaac gctgcatcgg aatttgcttt
    21061 tccgcttgtt ccttgacttt gtctgctaaa acttccgtca tatcagttgt gatgaatcct
    21121 ggcgcaatgg cattacatgt aatgcctcta gttgcagctt ctcgtgccac agattttgtt
    21181 aatcctacta cgccagcttt actagctgca taatttgctt gaccaatatt tccaatcaac
    21241 ccagatacac tagagagatt aatgatggcc ccttctcgct gcttcatcat ttttttcaat
    21301 acgtgctgcg tcatattgaa ggttccaatc aaattgatat ccagacattt tttgaaatct
    21361 tctgcatcca ttcgcatcac aagtttgtca tttgtgattc cggcattgtt tactaacaca
    21421 tggatcgatc caagcttttc ttctgcttct ttgatcattt gtcccgcttt ctcataatca
    21481 gagatgtcac cggaaactcc tacgcatttt acaccgaatg cttcgatttc ttctatcttt
    21541 tcttttggaa tctctccgcg accattcaaa atgatatttg ctccagcttt tgcaaaagct
    21601 aaggccatcg ctttgccgat tcctcttgta ctacctgtga taaatacatt ttttcctttt
    21661 acatccataa cgttcctcct tatagtattg ctattgtttc atcaaatgtc tgcttattct
    21721 caacacgcag aactggtatt gttttatcga tttttttcat aaatccgctt aatacttttc
    21781 cggggccgac ttcgatgact tgttcgatgc cgattgtttt taacttatgg atactctcat
    21841 agaaacgaac aggttgcatg acttgctgtt caagtagtcc cgcgattgtt tctttcttca
    21901 ttatttccgt tgttgtgttg cttatgatag gaaaagaagg ttcagagaat tgaatctgac
    21961 ttaggtcctt cgccagtttt tttgctgccg gttctaaaat agctgtatga aaaggcccgc
    22021 tgacatttag agggatcatt cttttgaacc ctttttcttt taatagtaaa acagcttcgt
    22081 ctactgcttt ttcttcgcca ccaatcacga tctgctgagg tgtattataa tttgcaggag
    22141 atacgatgcc gtacttgcta gcttcatgac agctttcctc gatcgtttca atcggcgcat
    22201 tcatcactgc aaccattttc ccgcttccag ctggggcagc ttctgtcata taagcgcctc
    22261 tttttgctac taacgcaact gcttcagtga aggatagcgc attactagct actaatgccg
    22321 aatattctcc taaactcagt cctaaagctg catctgggat gattccatgc tccttcaata
    22381 agcgataata cgcgatgctg actgttagaa tagctggctg tgtatattgc gtctgatcca
    22441 aacgttcatt ttctgtaaag caaagttcag ccatatcgta gccgagaatc tcacttgctt
    22501 catcaaatgt ctgctttaca acggcttctt catataaatc ttttcccata ccttgatatt
    22561 gtgcaccttg gccactaaat aagaatgctg ttttcatgtt tttctccaat atatccagaa
    22621 tatatgtttg attcttccta ttcaaaaagg ccgagacatt cctatttcag gaaatgtccc
    22681 agcccccaac ctaattttaa tgattatgca gtttgttttt ctacgtaagt cactaaatct
    22741 tgaaccgttt tgattccgtc ttctgtttcg attttcacgt caaattcatc ttcaatttca
    22801 ttgatgattt ggaataaatc caagctgtca gcttctaagt cttcttggat gtcagtagtc
    22861 aatttgattt cttcagtttc ttttcctaat tcttctgcga tgatttcttg aattttttcg
    22921 aataccataa taatatattc ctccgatatt gtttgctttt tatttttata atttaacagt
    22981 ctacaactcc atgagaatac ttccccatgt taaacctccg ccatagcctg taaatattac
    23041 tttctgctgt tttcctaagg tgatctttcc actagaaact gcttcatcca ataagatagg
    23101 gatgcttgca gcagatgtat tcccatattt atccatattg gtcaggaact tttcttgcgg
    23161 catctttact tttcgggcaa ttttctcaat aattctgagg tttgcctgat gcaaaagcag
    23221 ataatcaact ggtttgtctt tcatgacttc ttggatattg tttgcaacgt ctcgtacggc
    23281 aaaatcaaaa atatctctgc cagtcatttg caaataatac gaaccttctg aatcttctga
    23341 atagaacgga tttttatttt gctggtagcc agcggtcaat gagctacttc tggtaccgtc
    23401 agctgctaat ttttctttca gcaatttttt ctgtggattc gcttctaaga gaatcccacc
    23461 tgctccatcg ccaaacaaca cagcagtcgt acgatcagac caatcgatta gcttagatag
    23521 tgtttcacca ccaatcacca gtcccttagt tgagccgcag cggatcattt tttcagccat
    23581 gcttaacgca taaacaaatc ctgagcacgc agcactgata tcaaaagcga tagcatgagt
    23641 tgctcccaat ttcccttgta cttgtgctgc tacagatggt gtcgtgtaat cgggcgacat
    23701 tgttgctact aaaataaaat ccaaatcttt agggtttaga tttttctgtt ttatcaattt
    23761 ttccgccact aaaatgcata aatcagaagt ctcctgattt gttacacatc ttcgttcacg
    23821 aatacccgtc cttgacgaaa tccattcatc agaggtatcc atcatcttgg aaagatcatt
    23881 attagtaata atcttttcag gaaggacgct ggctgttgca gtaatcattc caaattgttc
    23941 cattaaatat cctcacttat agtcttgcaa gaagtcgtgc aagtttttca gtgcatccag
    24001 caaggcatgc tgttcctccg tagccattcc gtctaagatg ttcttgacca tttcacgatg
    24061 aaaatgctga tggacccgaa atagtaactt tccttttttc gttagtccca actttacaac
    24121 gcggcggtca tcttcactgc ggatacgttc gacatagcct ttttttacta acttgttaat
    24181 agcagtggtc aaggtcccaa cagtaattga tagttctttg gctacttctg aagttgtttt
    24241 tttcttgtac atcccgattg cctcgatcgt gtgcatctct gtgattgaca gatctttgaa
    24301 ttgagatttt ttcagctcag actcttcgat tgtcaaaata tcattgaaga cactaacaag
    24361 ataatcattt actgttttta aatctggttc catgttccac cgtccactaa ttagtttgat
    24421 tatcaaatct tttgatgttc aaactatatc ttaacttttt tc
//
LOCUS       DACENJ010000021        23073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.021, whole genome shotgun sequence.
ACCESSION   DACENJ010000021 DACENJ010000000
VERSION     DACENJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.021"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IW748_000634"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IW748_000634"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1103563.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IW748_000635"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IW748_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1103564.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IW748_000636"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IW748_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1103565.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IW748_000637"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IW748_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1103566.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IW748_000638"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IW748_000638"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1103567.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IW748_000639"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IW748_000639"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1103568.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IW748_000640"
     CDS             complement(8053..8523)
                     /locus_tag="IW748_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1103569.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IW748_000641"
     CDS             complement(8569..8994)
                     /locus_tag="IW748_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1103570.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IW748_000642"
     CDS             complement(9061..9330)
                     /locus_tag="IW748_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1103571.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IW748_000643"
     CDS             complement(9341..10936)
                     /locus_tag="IW748_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1103572.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IW748_000644"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IW748_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1103573.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IW748_000645"
     CDS             complement(14489..15049)
                     /locus_tag="IW748_000645"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1103574.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IW748_000646"
     CDS             15402..16376
                     /locus_tag="IW748_000646"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1103575.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IW748_000647"
     CDS             16546..17946
                     /locus_tag="IW748_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1103576.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IW748_000648"
     CDS             complement(18024..18746)
                     /locus_tag="IW748_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1103577.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IW748_000649"
     CDS             complement(18825..19331)
                     /locus_tag="IW748_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1103578.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IW748_000650"
     CDS             19454..21253
                     /locus_tag="IW748_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1103579.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IW748_000651"
     CDS             complement(21321..22895)
                     /locus_tag="IW748_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103580.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6317 a         5009 c         4217 g         7530 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaa
//
LOCUS       DACENJ010000022        22947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.022, whole genome shotgun sequence.
ACCESSION   DACENJ010000022 DACENJ010000000
VERSION     DACENJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.022"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            44..1378
                     /gene="rpoN"
                     /locus_tag="IW748_000652"
     CDS             44..1378
                     /gene="rpoN"
                     /locus_tag="IW748_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1103581.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            1581..2618
                     /locus_tag="IW748_000653"
     CDS             1581..2618
                     /locus_tag="IW748_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1103582.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            2659..3660
                     /gene="gap"
                     /locus_tag="IW748_000654"
     CDS             2659..3660
                     /gene="gap"
                     /locus_tag="IW748_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1103583.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            3770..4960
                     /locus_tag="IW748_000655"
     CDS             3770..4960
                     /locus_tag="IW748_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1103584.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            5064..5819
                     /locus_tag="IW748_000656"
     CDS             5064..5819
                     /locus_tag="IW748_000656"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1103585.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            5935..7233
                     /gene="eno"
                     /locus_tag="IW748_000657"
     CDS             5935..7233
                     /gene="eno"
                     /locus_tag="IW748_000657"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1103586.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            7632..8771
                     /locus_tag="IW748_000658"
     CDS             7632..8771
                     /locus_tag="IW748_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1103587.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(8838..9413)
                     /locus_tag="IW748_000659"
     CDS             complement(8838..9413)
                     /locus_tag="IW748_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1103588.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(9418..10392)
                     /locus_tag="IW748_000660"
     CDS             complement(9418..10392)
                     /locus_tag="IW748_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1103589.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEPICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(10416..10460)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            10675..11643
                     /locus_tag="IW748_000661"
     CDS             10675..11643
                     /locus_tag="IW748_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1103590.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            11740..12033
                     /locus_tag="IW748_000662"
     CDS             11740..12033
                     /locus_tag="IW748_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1103591.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            12151..12387
                     /gene="secG"
                     /locus_tag="IW748_000663"
     CDS             12151..12387
                     /gene="secG"
                     /locus_tag="IW748_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1103592.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            12499..13245
                     /locus_tag="IW748_000664"
     CDS             12499..13245
                     /locus_tag="IW748_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1103593.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            13248..15605
                     /gene="rnr"
                     /locus_tag="IW748_000665"
     CDS             13248..15605
                     /gene="rnr"
                     /locus_tag="IW748_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1103594.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            15627..16091
                     /gene="smpB"
                     /locus_tag="IW748_000666"
     CDS             15627..16091
                     /gene="smpB"
                     /locus_tag="IW748_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1103595.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            16393..17112
                     /gene="atpB"
                     /locus_tag="IW748_000667"
     CDS             16393..17112
                     /gene="atpB"
                     /locus_tag="IW748_000667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1103596.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            17165..17380
                     /gene="atpE"
                     /locus_tag="IW748_000668"
     CDS             17165..17380
                     /gene="atpE"
                     /locus_tag="IW748_000668"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1103597.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            17471..17995
                     /gene="atpF"
                     /locus_tag="IW748_000669"
     CDS             17471..17995
                     /gene="atpF"
                     /locus_tag="IW748_000669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1103598.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            17982..18524
                     /locus_tag="IW748_000670"
     CDS             17982..18524
                     /locus_tag="IW748_000670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1103599.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            18547..20103
                     /locus_tag="IW748_000671"
     CDS             18547..20103
                     /locus_tag="IW748_000671"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1103600.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            20117..21019
                     /locus_tag="IW748_000672"
     CDS             20117..21019
                     /locus_tag="IW748_000672"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1103601.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            21042..22448
                     /gene="atpD"
                     /locus_tag="IW748_000673"
     CDS             21042..22448
                     /gene="atpD"
                     /locus_tag="IW748_000673"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1103602.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            22462..22884
                     /locus_tag="IW748_000674"
     CDS             22462..22884
                     /locus_tag="IW748_000674"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1103603.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         7723 a         4156 c         4914 g         6154 t
ORIGIN      
        1 gtaactaaac agataaaaaa ttacaagggg attggacaaa aaaatgaaat ttgagcaaaa
       61 tttttcacaa aaacagcagc agacacagaa actagccatg acgcaacagt tgcaacagtc
      121 catccaagtt ttacaatata acagtgagga gctgttggca ttcgttgaga atcaagcaat
      181 ggaaaatcca ttggtggaag ttgttgagcc ggaatggcag ccagattaca taaaagcttc
      241 ttcatcttct tatgaaggag aagagacaaa ttacctaaat cagattcctg atacaaaagg
      301 gtctttattt gattcattga tcgaacaagt ccatttgaat taccgagata cttttttgag
      361 gaaaatcgtt ctttatcttg tagaatacat cgatttgaac ggatttttga cgattagttt
      421 agaagaagcc ggaaaagaga ttggagctac gcctatccaa atgctcgatg cgttgactct
      481 gattcagcaa ttagaaccag ctggagtagg tgcacgtagt ttgcaagaat gtctcatgct
      541 tcaaacagaa cgagatgatt atgcgccgga attagcttat atcgtgttgg aagaatgttt
      601 tgaggaactg gtggagcgaa aatggaaaga aatcgcacaa cgtttttctg ttgatctaca
      661 tgctgttcag cagatattcg actatttaca aacgttgagt ccaagccctg gccggatttt
      721 cgaccgtagc agcgaattgt acattattcc tgatgtccgg gtattagtgg acgacgataa
      781 aaatgtacaa gttatctcaa accgaaaaaa tcagccaaat atacgctttc aagagagtta
      841 cttcaaacaa atgtcacaac aagcagataa agaaactgaa aattatttaa aagaacggaa
      901 acaggaattc gaatggttga agaaaacgat cctccaacgt ggggatacaa ttttgcgggt
      961 tgctcaagtc attgtttccc ggcaaaaagc tttctttatc gacaaggaac gtccaatcaa
     1021 gcctcttacg ttaaaggaag ttgcaactga gatagatgtg catgaatcca cggtcagtcg
     1081 tgcagtcaac gggaaatatt tggaaacaga tttcggtgta tttgaattaa aaaaattttt
     1141 cactacacga attcctacga atagtacaga acagacagaa gacctttcag cagatacagc
     1201 gaagaaaaag ctgcaggaat tagtcgatca agaagacaaa aacaagccgt tgtcggacca
     1261 gaaattggtt gaattgctga aaaaagatga gatagcgatc tcaagacgaa cagtagcaaa
     1321 atatcgtgat ttattaggaa tcccgagttc atcaaaaaga aaacgttatg ataactaatc
     1381 agtcaaaaga ctagaaaaag tatttcactt tttctagtct ttttagttga aaaacgagag
     1441 caaaactgtt agacttacag tcgtgaggtt gagatttccc tttattgatg tgaaattcga
     1501 atagttaatt tcttttttta tcgtatatgg gtcgttttaa gtctacattg gacgtaaagt
     1561 gtccaactaa ggagatcgct atgctggatg aaataaaaat gattgaatct gttgcaccag
     1621 atattatcga agtgttgcaa gaacgctata aaattttacg aaatatctat tggatgcagc
     1681 cgattgggcg tcgaagttta tcggaaagta tggggatcac agaacgagtc ttacgaacag
     1741 aaacagacct tttgaaaaat ctgaatttga tcgacacctc aaaaagcggt atgacgctga
     1801 caactaaggg cgaagaagtt tatcaaagcc ttgaaaactt catggatcag cttttaggta
     1861 cgcatcaaac cgaacaacag ttggcagaat attttggtaa ccaacgctgt atcgttgttt
     1921 caggaaacag cgaagagcag accaaagtgg ctgatgcatt cggcgaagcg ctgagcgaag
     1981 ccttagaccg actacttcca gaaggcgaga atatcatcgc tgtgatggga ggaactacta
     2041 tggctgtggt agccgaacaa ttgtctaatc tggaaaataa gaaacggcat aacctgtttg
     2101 tccctgcaag aggcgggatt ggtgaagcga taactgtaca ggcaaattct gtcagtgcaa
     2161 gaatggctgc taaagcaaac ggtaaccacc gcgcacttta tgttccggaa caattaagtc
     2221 tggcaactta caattcttta ctcaatgagc catctatcca agaagtctta aacttgatta
     2281 gtgaagcgaa ttgtgttatt catagtattg ggcgtgcatt acacatggca gcacgtcgta
     2341 agatgactga aaaagaatta gtcatgctga aacaagcaaa tgctgttgcg gaatcttttg
     2401 gctacttttt caatgaaaaa ggtgaagtcg tttataaggt ccctagaatc ggcatagaat
     2461 tgcaagattt ggaaaaagtc ccgatcatca tggcaatcgc gggcggaaaa tcaaaagcta
     2521 aagcaattcg agcttatatg aaaaatgcac caaaacaaac gtggctcatc actgatgaag
     2581 ctgctgcaaa tgagatttta aaaggggtaa ccctttaaaa taaatatttt tttgattttc
     2641 ataaggagga aatcttaaat gacagttaaa gtaggtatta atggttttgg acgtattgga
     2701 cgtctagcat tccgtcgtat ccaagatgta gatggaatcg aagtagtagc aatcaacgac
     2761 ttgacagatg ctaaaatgtt ggcacacttg ttaaaatacg acacaactca aggacgtttc
     2821 aacggaacag ttgaagttca tgaaggttca ttcaacgtaa acggaaaaga agttaaagta
     2881 ttagctaacc gcaacccaga agaattacca tggggcgaac taggcgtaga tatcgttcta
     2941 gaatgtactg gtttcttcac ttctaaatca gcagctgaaa aacatttaac agctggtgct
     3001 aaacgcgttg ttatctctgc tcctggcgga aacgatgttc caacaatcgt ttataacaca
     3061 aaccacgaaa cattaactgg taaagaaaca gttatctcag gtgcttcatg tactactaac
     3121 tgtttagctc ctatggctaa aacattgaac gacaaatttg gtgttgttga aggattaatg
     3181 acaactatcc acgcttacac aggtgaccaa atgactctag acggacctca tcctaaaggt
     3241 gacttccgcc gtgcacgcgc tgctgctgca aacatcgttc ctaactcaac aggtgctgct
     3301 aaagctatcg gtttagtaat tcccgaattg aacggtaaat tagacggagc tgctcaacgt
     3361 gttcctgtac caacaggttc attaacagaa ttagtaacag ttcttgaaaa agaagtaact
     3421 gttgacgaaa tcaatgcagc aatgaaagaa gcttcaaacg aatcttacgg atacaacaca
     3481 gacgaaatcg tttcctcaga tatcgttggt atgacttacg gttcattatt tgatgctaca
     3541 caaactaaag taatgacagt tggcgacaaa caattagtta aaactgttgc ttggtacgac
     3601 aacgaaatgt catacactgc acaattagta cgtactttag aatacttcgc taacttataa
     3661 gatagacaaa cgtacaagtg taaattatag acatgtacat ttaaaagcgg ggaagcaatc
     3721 gcgcttcttc cgcttttttt gataataagt ttattcagga ggaaataaaa tggctaagaa
     3781 aacagtgaaa gatatcgatt taaaagacaa aaaagttctt gtccgtgttg acttcaacgt
     3841 tcctttgaaa gacggcgtga tcactgacga cactcgtatc aaagcagcat tgccaacaat
     3901 caactacgtt ctagaacaag gcggaaaagc aatccttttc tctcaccttg gacgtgtaaa
     3961 aacagaagaa gataaagaag gaaaatcttt agctccagtt gcaaaacgtc taggcgaatt
     4021 attaggtaaa gaagtaacat tcgtacctga aactcgcgga gaacaattag aagaagcaat
     4081 ccgcaacatg aatgacggcg acgttgtcgt attcgaaaat acacgttttg aagatattga
     4141 cggtaaaaaa gaaagcaaaa atgatacaga actaggtaaa tactgggctt cattaggcga
     4201 tgtatttgtt aacgatgctt tcggtacagc tcaccgtgca catgcttcta acgtaggtat
     4261 cgcttcaact ggtatcccaa cagttgctgg attcttgatg gaaaaagaaa tcaagttcat
     4321 cggtgaagca gtagaagaac caaaacgccc aatgattgca atccttggtg gcgcaaaagt
     4381 ttctgataaa atcggcgtaa tcgaaaactt gattcctaaa gcagataaaa tcttaatcgg
     4441 tggcggaatg acttacacat tctacgaagc aaaaggaatc aagatcggta actctctagt
     4501 agaagctgat aaagtagaac tagcaaaaga attaatcgaa aaagcaggcg acaaacttgt
     4561 cttgccaatc gacaatgtct gtgcaccaga attttcaaat gatgttgaaa cacaagtaat
     4621 cgaaggagac attcctgatg gtttgatggc tttagacatc ggcccagcat cagtaaaatt
     4681 atttgctgat acattacaag gtgcaaaaac agttgtttgg aacggaccta tgggtgtatt
     4741 tgaaatgagt aactttgcta aaggtacaat cggtgtttgt gaagcaatcg ctaacttaga
     4801 aggcgcaaca acaatcatcg gtggcggaga ctcagctgca gcagcagaac aattaggatt
     4861 cgctgacaaa ttcacacaca tctcaacagg tggtggcgca agcttggaat tgcttgaagg
     4921 taaagaatta ccaggactag cagcaatcaa cgataaataa acgaccaagt ttgctcaatt
     4981 ctgacgagta atcgatcaaa aggtgaaggg agttttcatg actccctagc catttttcaa
     5041 taaattaagg agtgtgttcc tcaatgcgta aacctattat cgcaggtaac tggaaaatga
     5101 acaaaactgc atcagaagca aaagaatttg ctgaagcagt aaaaaacaaa atcccttcaa
     5161 atgacaaagt tgattcagtc atcggatctc ctgcattatt cttacaagaa ttaatgacat
     5221 cagctgaagg tacagaacta aaaatctcag cacaaaactg ctactgggaa aactcaggtg
     5281 cgttcactgg tgaaacttca ccagcagctc ttgctgatct aggtgttcat tatgtgatca
     5341 tcggacattc agaacgtcgt gaatatttcc atgaaacaga cgaagacatc aataaaaaag
     5401 caaaagctat ctttgcaaat ggtatgacac caatcctatg ctgcggcgaa tctttagaaa
     5461 cctatgaagc aggcaaaact gctgaatgga tcgaaggaca aattacagct ggtttagctg
     5521 atctttcagc tgaacaagtg tcaaacatgg ttattgctta tgaaccaatc tgggcaatcg
     5581 gtactggtaa atcagctgac gcacaaatcg ctgacgaaat ttgtggagtt gtacgtcaaa
     5641 cagttgaaaa actttatgga aaagaagtat cagaagcagt tcgtatccaa tacggcggtt
     5701 ctgtaaaacc agaaaatatt gctgaataca tggcaaaaga aaatgttgac ggtgctttag
     5761 tcggtggcgc aagcttgcaa gcagattcat tcttgtcatt gttagacgct gtaaaataat
     5821 tgttgaaaat ttgtgaaaaa tgcaagaaat gtttgcacta atgagcaaaa ttaactaaaa
     5881 tcgtatatga gggaaatccc ttaggaaaaa acaaactcaa aggagagaca aaacatgtca
     5941 attattactg atgtttacgc tcgcgagatc ttagactcac gtggtaaccc aacaatcgaa
     6001 gtagaagtat acacagaaag cggagctttt ggccgcggaa tggttccttc aggtgcttca
     6061 actggtgaat acgaagcagt tgaattacgt gacggagaca aagctcgtta tggcggtaaa
     6121 ggtgtaacta aagctgtcga caacgtaaac aacatcattg ctgaagcaat cattggctac
     6181 gatgttcgtg accaaatggc tatcgacaaa gctatgatcg ctttagacgg aactcctaac
     6241 aaaggtaaat taggtgcgaa cgctatttta ggtgtttcta tcgctgttgc acgtgcagct
     6301 gctgactacc tagaagtacc tttataccac tacctaggcg gattcaacac aaaagtattg
     6361 ccaacaccaa tgatgaacat catcaacggc ggatcacatg ctgataactc aatcgacttc
     6421 caagaattca tgatcatgcc tgttggcgct ccaacattca aagaagctct acgtatgggt
     6481 gctgaagtat tccacgcatt agcttcaatc ttgaaagctc gcggattagc aacttctgtt
     6541 ggtgacgaag gtggatttgc tccaaacctt ggttcaaacg aagaaggttt tgaagtaatc
     6601 atcgaagcaa tcgaaaaagc tggctatgta cctggtaaag acgttgttct tgctatggat
     6661 gctgcttctt cagaattcta cgacaaagaa aaaggtgttt acgtactagc tgactcaggc
     6721 gaaggcgaaa aaacaactga cgaaatgatc aaattctacg aagaattagt ttctaaatat
     6781 ccaatcatct caatcgaaga tggattagac gaaaacgact gggacggatt caagaaatta
     6841 actgacgtat taggcgacaa agttcaatta gttggtgacg acttgttcgt aacaaacact
     6901 caaaaattat ctgaaggtat cgaaaaagga attgctaact caatccttat caaagtgaac
     6961 caaatcggta cattaacaga aactttcgaa gctatcgaaa tggctaaaga agctggctac
     7021 acagcagttg tatctcaccg ttctggtgaa acagaagatt caacaatctc tgatatcgct
     7081 gttgcaacaa acgctggcca aatcaaaact ggttctctat cacgtactga ccgtattgct
     7141 aaatacaacc aattactacg tatcgaagac caacttggtg aagttgcaga atacaaaggt
     7201 ttgaaatctt tctacaactt aaaaaacaaa taattctttt ctaatgaaga tacatgatcg
     7261 actattgaaa gatagtcgag tatgatcact tagttaagta atcgaactct ctacttgctg
     7321 aaatatcagc aggtagagag tttttttgca actagcttct gtatatgatg tttacaaaga
     7381 atggtacaag aaaagatcat ccttgaaaaa ggaaagctaa caataactag catcatataa
     7441 gagcttttaa ggatagaaca gtagaatagg aatctatgag caagtcctct cgtataaata
     7501 aaataggagg gaagtagcag catatttttc ataaagtggc tctctttctt catttttact
     7561 ttggttgctc ctatttgaaa attcgagcac actcaaatac aataaaaaga aatggcaagg
     7621 gagaggatta gatgaaaaaa gaagaaaaaa tcgcgatttt acaagagatc atccgaatta
     7681 aatcagtgaa tggaaacgaa ggtgaagtag cagcctattt gaataagctt ttggcaaaac
     7741 atgacataac aggagagatt gtttcttaca gagatggtag agataatttg attgctcgtt
     7801 accaaaaggg ccaatcagga aaagtgttag gcctatctgg gcatatggat gtcgtggcgg
     7861 ctggtgatga atcgtcttgg acctatgcac cctttgcagc agaaatccat gggaaccgtt
     7921 tgtacggacg tggtgctaca gatatgaaga gtggcttggc agcaatggtg attgccatga
     7981 tcgaactgaa agagtcagga aaaccattta acggaacagt caaattatta gccactgtcg
     8041 gagaagaagt gggtgaacta ggtggggaac agctcacgaa agcaggctat gtagatgact
     8101 tagatgcctt gatcatcgga gagccaacca attacagttt gatgtatacg cacatgggat
     8161 caatcaacta tacagtcacc tcgcacggaa aagaagcgca tagctctatg ccagaccaag
     8221 gttataatgc aatcaatcac ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc
     8281 acctcgctga aacaatcgag aatcctgtcc taggaaaaac aatccataat gttacattga
     8341 tcagtggcgg gaatcaagtc aacagcatcc caagccatgc gcagctccaa gggaacattc
     8401 gatcgattcc agaataccca aatgataaaa tcattgcatt gctgcaatca atcgtcaatg
     8461 aattgaatca agagacagac tatcatttgg aattgatgat cgattacaac aaaatacctg
     8521 tcaaagctga tccagattct cctttgattc attgcattca gcaacaattc agccagccat
     8581 tacctttagt tggagcggca gcaacaacgg atgcagccga atttacgaaa gctaaccatt
     8641 ccttcgactt tgtcgtcttt gggcctggtg tcgttacttt gcctcatcaa gtagatgagt
     8701 atgttgaaat cgataattat ttggatatga tcgaaaaata tcaaggaatt attttatcct
     8761 acttagcgta agattttttt aataaatgaa aaaacagagc cagattagca ttattttgct
     8821 aatccagctc tgtttattca gcctattttt ctttttgaat atatcaagcg ttgtggcaac
     8881 atagtgatga ctaatgaaac aacgacgata gacaacaaac gatcaaggta gtctgtacct
     8941 gcctgtacca agatcacact tgttgtctga cttaaaccta acccatgtaa tagctgaacg
     9001 atcatactgg atcctgagga ggtgatcccg ccaaataact gaacggtaat gatcgaagaa
     9061 acgatcgttc caggtaccga caaaatcaaa gcgagtaata acagtttcaa gctgttttta
     9121 tctttgccga gatatcttgg caaaatcaat ccagcaagca agcctgtgag aatttggatt
     9181 ggcgaataat acagagaaaa aatatctgta gtcactccac ttaaaatacc gctggacaat
     9241 cctgtcagca ttccaggtac aggcccaagc aatgcaccag caaaaatcgt tccgatagaa
     9301 tcgagataga ttggcaaacg aagccataaa gcaatcgttc ctccaatata attgatccca
     9361 atacacaaag ccatcactgt aagtacgcga gttgccattt tgtttttatt cattttgtta
     9421 gcccatcttt aacctttcca aatctttcat aatcgtttct tcttgtgcat ttaaaagaac
     9481 ggttaaaaac tttcggaaaa aaagacgagt atctactttt gtcataattt tactgttagc
     9541 aggtttctgc caaaattcat aacgatcaac caatgtttgt ccacgactta ttccttgagt
     9601 ttccacagca gtgaagcttt tgaaaccttc acagatgggc tcctcgataa aataagcaac
     9661 agctaacgga tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa
     9721 ataaaaacga gtgatcgctt ttagatactc tccctcttca ggattcattt gacagcagta
     9781 ttcgaggata gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat
     9841 cgtttgtttg agattctcga atacataaga agctgcttca ggatcacacc agtaattgta
     9901 ttcagcaact ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg
     9961 ttccatatgt ttcccaatat ttgggttgac tttcaaagca gaagcgatat tggtcaaagg
    10021 acccaaagca atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac
    10081 ggcatggaga ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc
    10141 atccatacca tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc
    10201 ttgataaaca ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt
    10261 acctatatct gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg
    10321 ggatttgaca gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat
    10381 tacttttcgc attatttcca atctcctttc tttagagata cttagttttt tattctggga
    10441 agtttccgaa ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg
    10501 cttttattgc gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta
    10561 aatgatacaa tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat
    10621 cggtacattg gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt
    10681 attttttatt tcattcttat tctgataatt atagggatca gttaccattt tctttcttat
    10741 ttttatgcaa agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac
    10801 actggtcatc gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa
    10861 ttagaagaag agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat
    10921 gacgggttga aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc
    10981 atttgcgtac atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga
    11041 tatgcagaga aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa
    11101 ggagaaatca tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc
    11161 atcttagatg aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct
    11221 acgatcatga tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac
    11281 agcggatatg tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt
    11341 cctaaaaaaa tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc
    11401 ttaaagcaag catcagctac gagacagctt ggcagcaatc atctgccttt attagtcatc
    11461 catggtgaaa gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg
    11521 gcaggggata aagcactgct gcttgttccg gaagcagaac acttggaagc atcgatgaaa
    11581 gatccgaaaa cctattggac agtgattttt tcatttattg aacaaagaat aacgatgaat
    11641 taattttctc ttcttttttg taagtaattc gaggataaat aattgtttat agatcaggag
    11701 actgctaaaa taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga
    11761 aatcaaagaa gaaaaaatcg tcttgcttta ttcaatgatg aacaacaaga gatcggagaa
    11821 atgacatggt cagatgcggg cccagacatc atgatcattg accatacatt tgtagatcca
    11881 gcatatcgtg gtcaaaagtt agctgaaaag ctagtgtata ctggagtaga attagcaaga
    11941 cgagacggga aaaagatcat tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa
    12001 ccagaatatc aagacgtctt acgtcagtca taagaataac agctgaaagt tttgaacttt
    12061 cagctgttat tccatttata tagagttgtc acggactttt atctatgata aagttaaaga
    12121 gacgatatct tttgggaagg aggctgcgat atgtataatt taattttggg aatcgtgatc
    12181 gttttatcga ttgttatggt cattgcgatt atgatgcagc caagcaagca aaatagtgca
    12241 gcgagtgcat ttaccggcgg tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt
    12301 gaagctgtca tgcagcgatc aactgcagtt atgggagcta tctggatgat cttattattt
    12361 gtcctagcat atttatcttc gaagtagttg aataagcctc tcgcaaaacg gcgggcggct
    12421 ttttttattg caaagagaac gcttaagaag aagttatgaa agaaactatg gtaaaatgag
    12481 gcgaaagagg tgagacgagt gagacaatta ccaaaacccc tttatgcaaa acatggaaag
    12541 cgagcggttt tgctgcttca cgcttactca ggaagtccaa acgatgttcg aatgctcgca
    12601 cgatttttgg aaaaagcaga ttatacagtt tatgcgccgc tttttaaagg acacggaaca
    12661 atggagccat atgatatctt gcaagaaaaa gcagaaagct ggtgggcaga cactaaaaaa
    12721 gccatccatt ttttacagtc tgagcagttt tctgatattg ccgtattagg attatcaatg
    12781 ggcggcatct ttgctgtacg agcactagaa gaagaatcag tgattggcgg cggttttttc
    12841 tgttcgcctt tgtctcctgt aaaaacaaac gtgcctgaaa actttgaaaa atacgtacgt
    12901 caagtgctga agacagcagg taaatcagag aaagaaatca atgaaaaagc agttgcttac
    12961 cgctcgttag ctgaacaaca attaatggat atacaagatc aggcagcaat cgtagaaagt
    13021 aggttgtcag atatccaaca gccgatattt ttagctcaag ctgggaaaga tgaaatgatc
    13081 gaccctaatg gcgtatttga aactgcacgc aagttgagcc gtcagcgagt gacacttcaa
    13141 tggtatcctg aaagtggtca tgtgattaca gttggtacag caagacgcga attagaaaaa
    13201 gacgtcttgg aatttttaga aaaattacct tggaatgagg aataaatatg acaaaagaaa
    13261 cgataaaaga gaaaattctc tttttcatgg aaagcagtcg caaacgcagt ttttctatgg
    13321 aagaaatcgc tgaaggttta gggttccaaa aaagcgatga ttttaaatta ctagtacaaa
    13381 cgattgctca aatggaaaga gaacagagta tagttttcaa taaaaaagga aaagtgaaac
    13441 ttcctttaaa accaatcttg atcgaaggaa catttcgagc aaatgaacga ggatttggat
    13501 ttgtaacgat tgatcccgaa gaagatgaca tctatatccc aaaagaagcg actggttttg
    13561 caatggatgg agacaccgta gcaatcgata tcatgaagcg agcaaataca gatatggatc
    13621 gcggagcaga ggggaaagta gtcgagattc gagaccgggc gactactcag ttagcagggg
    13681 aattcgttgc ttattcagaa gaagaaatac aagaaacaga tctttacggc gttgttatcc
    13741 cgaaagatag aaagctcaac caatttaaag tatatgcagc agcagaggga gttcgtccag
    13801 ttgacggaag tattgtgctg atcgaactga cccattaccc tgaaaaaggg tatgcgacta
    13861 gtttagaagg gataatcaag caagtgattg gtcataagaa tgaccctggc atggatatcc
    13921 tctcaattgt cgtggctaat ggtatcccta cgaaatttcc tgatgatgta ttgtctgaag
    13981 cagatgaagt tccagatcaa atcagcgaaa aggatataac tggacgtaga gatttaagaa
    14041 atcagctcat tgttacgatt gatggagaag atgcaaaaga tctagatgat gcagttacgg
    14101 ttaaaaaatt agataacggt aattattttc ttggagtaca cattgcagat gtttcttact
    14161 atgtgacaca aggcagtcaa ttggacagag aagcatatga aagagggaca agtgtatatt
    14221 tgactgatcg agtaattccg atgatcccgc aacgtttatc gaatggtatc tgttcactga
    14281 atccccatgt tccaaggctg accatgagtt gcgaaatgga gattgatcca aatggacaag
    14341 tagtctctca tgatattttc ccaagtgtga tccaaacaac agaacggatg acctacacag
    14401 ctgtcaatca aatcttagaa gatcaagacg aagaaacgat gcaacgctat gctcagctcg
    14461 tacctatgtt ccaagaaatg ggtgaattgc accgaatcct tgaaacgatg cgaatcaaac
    14521 gtggcgcgat ttcttttgag gatcgcgaag cgaaaatcct tgttgatgct gaaggacacc
    14581 caaatgacat ccagctaaga agcagaggaa ttggtgaacg cttgatcgaa tctttcatgc
    14641 ttgcggccaa tgaaacggta gctgaacatt ataataaatt aaatctgccg tttatttacc
    14701 gaatacatga acagccaaaa gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt
    14761 tgggcattct ggtccgagga acgaaaggaa cgatcacacc aaaagattta cagcatgtga
    14821 tcgaaggagt agaagacaaa ccagaagctg cagttatcaa tactatgttg ctaagaagca
    14881 tgcaacaagc acgctattca gaagacaatt ttgggcatta tggcttagcg gcagattact
    14941 acacacactt tacatctcct attcgacgct atcccgatct aatcgtccat cgtctgatcc
    15001 gttcttacag tgaagataaa agtgaaagca atcaaagttc gtgggctgcg gaacttcctg
    15061 aaattgcaga tcatagttct aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag
    15121 attccatgaa gaaagcagaa ttcatgatgg acaaagtggg agaagaattc gatgggatca
    15181 tcagctctgt tgtgaaattc ggcctgttta tcgaactgcc gaatacagta gagggattga
    15241 tccatatcaa tgaattgaaa caggactact tccattttgt tgaaaatcat ttagctcttg
    15301 taggtgaaag aactggtctg acttttaaaa tcggacagaa agtgcgagta aaagtagtca
    15361 aagctgatcc agatgaacga gcagtagatt ttgaacttgt ttccgcagaa gaggttacag
    15421 cgttggaacg gccaaaaaca cgaaatggaa aatcaggaaa acgcgaagga aaacaagcag
    15481 gaaaacgccc agaaagaaat ggcaagaaga acaaacgata tagcgatagt aagcaaacgt
    15541 tcggtaagaa aaaagggaag aagccatttt acaaaggtgt taaaaaaaag aaaaagaaaa
    15601 aataaaatga gttggaggaa acagctatgc caaaaggcga gggaaaatta attgcacaaa
    15661 acaagaaagc tcgccatgat tattcgatca tcgacacgat ggaagcaggg atggtcttgc
    15721 aaggaaccga gatcaagtcg atacgaaaca gccggatcaa tctaaaagat ggatttattc
    15781 gcgtccgcaa cggagaagct ttcttgcata atgttcatat cagtccttat gaacaaggaa
    15841 atatttttaa tcatgatccg ttgcgcacga gaaagttatt attacacaaa aaacaaatca
    15901 tccggcttga aaatgaattg aaaaatactg gaatcactgt tgttccttta aaagtctata
    15961 ttcgtaacgg ctatgccaag gtattgattg gtctggcgaa agggaaaaaa tcttatgata
    16021 aacgggaaga tttgaaacga aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt
    16081 tctctagata atttcattat cctttttcgc atattcggtg cttttgcttt tacttaatga
    16141 taaaatttgt tagcatttaa tctgataaaa catgattatc ataggttttt tcttagatca
    16201 ggagcaccgt gtataaagaa aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa
    16261 ttttcatgaa aatgttttga attttctgtt tcatggtggt atgataccgt gtggttagaa
    16321 accgattgga aaaaaccaga tacgcacatg aattgatttt atgtgaattt cggggaaaag
    16381 aggtgaaatg gattggatga acgctcgcta acgttccaca ttggaccaat ttggtttgat
    16441 ggtacagttt gtataatggt gttactgacc tgtttgattg ttttcttctt agcttacttt
    16501 tttacaagaa atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt
    16561 gattttagtc gaggaatcgt aacagataat ctgcctagaa aagaattgag caatttccat
    16621 ttgttggcat ttacattgtt tttatttgtt tttgttgcaa acaacatcgg tttagtaaca
    16681 aaaatcgttt tacctaacga aacaacactt tggaaaagcc cgacagcaga cccgtttgtt
    16741 actttaactt tggcatttat aatgatctta cttactcact tatttggggt gaaaaagtta
    16801 ggcttcaaag gatatttcgt aaattcgttc ttgaaaccat atagttttat gtttccgatg
    16861 aaactgattg aagaatttac aaacttatta acacttgctt tacgtcttta tgggaatatt
    16921 tatgcggggg aagttttgct aaccttgatt gcaaaaatgg taataagctt aggctggttc
    16981 tcattgccgt tagcaattcc attagagatg gtctggatcg cattctcctt gtttatcgga
    17041 agcatccaag catttgtctt cgttacttta tctatggttt atatgagcca taagattgaa
    17101 gtggaagaat aatattcgtt ttaaaaacaa actactattt atctaattta ggaggaaaac
    17161 aattatgaat tatatcgcag cagcaatcgc aatcatggga gcagcaatcg gagccggtta
    17221 tggtaacggt caagttatct ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc
    17281 tggtcaattc cgtacaacaa tgtttatcgg ggtagctcta gtcgaagctg ttcctatctt
    17341 aggggtagtt gtcgccttga ttctagtttt tggtgtttaa ttgaatcagt attaaggcag
    17401 aaacaatttg gtttgttact gcttttttac atgaaaacta gaagtacttc aaagaaagga
    17461 aggctcgcct atgctgaatc aattggcaat tgcagaagtt ggcaatgcga tgttaggaaa
    17521 tgtcatcgtt gtcagcggct catttttgat actactggct ctcttgaagc attttgcttg
    17581 gggaccaatc agcgatattt tgaaaaaacg tgaagacaag atcgccaatg atttggattc
    17641 tgcagaacaa tctcgcatca actcagcgaa aatggaacaa gaacgcgaac aacaattgtt
    17701 agcctctcgt tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg gagaattaag
    17761 ccgccaaaat attttgaagg atgctcaaga agaagcagct cgtctaaaaa gtaaagccca
    17821 agctgatatc actgtagaac gtgattcagc gctgaactct gtaaaagacg atgttgcaga
    17881 actatctctt caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag aaatgcacga
    17941 atcattaatc aatcaatata tcgaaggtct aggttcttca aatgaaacta gataaatata
    18001 cagtaggaag acgttacggg aaagctttat ttgaactggc gatcgattca aatcaagcag
    18061 aagaaatcta tcaagatctc gtaagcttac gtcaaatcta tgaacaagta cctggattag
    18121 gtaatatgtt aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc atggacaagc
    18181 ttgttagtgg ctatgatgga atcgttaaaa actttttaga agttgtttat gcgtataacc
    18241 gaatggatga tcttttgttc atgatcgaag aatatgaacg tcgatatgat gaacatcaag
    18301 gtttgctgtt gggaagtgtc acaacagcag ttccactttc agacgaacaa cgaatacaat
    18361 tagaaaagaa tgttgcaaaa acaatgaact atcaatcagt cgaattaaaa caaatcgttg
    18421 attcttctat tattggtggc gcgattgttg aagctaacca tcgagtaatc gacggaagca
    18481 ttcgcacgca attagaaaat atgcgtaatc aattaaatag atagtacaaa gaggtaggtg
    18541 aatcgaatgg ccatcaaagc agaagaaatt agtgccttga ttaaagaaca aattgaaaat
    18601 tatcaaaacg tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc
    18661 gctcgtgcac atggtttaga aaacgcgatg agtggtgagt tgcttgaatt ttcaaatggc
    18721 tcatacggaa tggcgcaaaa cttagaatca aatgatgtag gtatcattat ccttggcgat
    18781 ttcgagtcca ttcgtgaagg agataaagta aaacgaacag gaaagatcat ggaagttcca
    18841 gttggagagg ccttgatcgg acgggtagtt aatccgctag gtcagccaat cgacggacta
    18901 ggtgaaatcg ttactgacaa agctcgtcca gtagaagcaa tggcaccagg cgttatgcaa
    18961 cgtaaatctg ttaacgaacc aatgcaaaca ggtctaaaag caatcgacgc tcttgtacca
    19021 atcggacgtg gccaacgtga attagttatc ggtgaccgta aaacagggaa aacttctatt
    19081 gcgatcgata cgatcatcaa ccaaaaaggt caagatatga tctgtatcta tgtagcaatc
    19141 ggacaaaaag attctacagt tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg
    19201 gattacacaa tcgttgtgaa tgccggtgcg tctcaaccag cgccgttgct ttatatcgca
    19261 ccatatgctg gtactgcaat gggtgaagag ttcatgtaca acggtaaaca tgtcttgatc
    19321 atttttgatg atctttcaaa acaagctgtt gcttatcgtg aactttcctt gctacttcgc
    19381 cgtccaccag gtcgtgaagc ttatccaggg gatgttttct acttacactc acgcttattg
    19441 gaacgtgcag caaaattaag tgatgattta ggtggcggtt caatgactgc cttaccattt
    19501 gttgaaacac aagctggaga tatctctgct tatatcccaa caaacgttat ttcgatcact
    19561 gatggacaaa tcttcttaga aagcgacttg ttctacgcag gtactcgtcc agcggttgat
    19621 gccggactat ctgtatctcg tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt
    19681 gctgggactt tacgtctaga cttagcaagt taccgtgaat tagaagcatt tacacaattc
    19741 ggttctgact tggatgcagc aacacaagca aaactaaatc gtggacgccg tacagtagaa
    19801 atcttgaaac aaaaattgca tgcaccgcta gctgttgaaa aacaagtagt gattttgtat
    19861 gctttgacac atggattctt agatagtatc cctgtagaca gtattttaga cttcgaacat
    19921 gaattattcg aatatctgga taccaatcat tcagatatct ttgaaacaat ccgtacaaca
    19981 aaagatcttc cagaagaaga agcgttgaac agtgcgattc aggaatataa agacatgttc
    20041 ttagctacaa aaggaaataa ttcctctaca gaagataaat tgaaatccat tcaaaatgca
    20101 tagtgaggtg agatgaatgg gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac
    20161 gaaaaaaacg agccaaatca cgcgggcaat gcagatggtg tctgcttcta agttgacaaa
    20221 atctgaagct tcttcacaga agtttcaggt ttatgcaaca aaagtaagag aaatcgttac
    20281 gcatttgact gctactcagt taaatgatat tgcctcagaa aatcctagag gggatatcaa
    20341 ttacaacagc atgctgctca gccgtcctgt taagaaaact ggttatatcg tcatcactgc
    20401 agatggcggt ttagtcggtg gatataacag ctcgatattg aaacaaacga tgtctatttt
    20461 agaagaagac caccaatcac ccgatgatta cgtgatgatc gcaattggcg gaactggtgc
    20521 agacttcttt aaagcacgcg gtatcaatct agcatatgag ctgcgcaatc tatctgatca
    20581 gccaagcttt gatgaagtcc gcaaaatcgt aaatatggca acaactatgt accaaaacga
    20641 agtattcgat gaattatacg tatgttataa ccatcatatc aattcgctaa ctagtcagtt
    20701 tcgtgtagaa aaaatgctgc caatctcaga cttagatcca gaagaagcaa ctactttcga
    20761 acaagaatat atttttgaac cttcaaaaga agaaatcttg tctcagttat tgcctcagta
    20821 tgctgaaagt ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg
    20881 tatgacagca atgaaaacag caactgataa tgctgctaat atcattgatg agctgacggt
    20941 ttcttataac cgtgcaagac aaggggctat tacccaagaa attaccgaaa ttgttgccgg
    21001 agcttcggca ctagaatagt attgagattg gaggaaaaaa gatgagttca ggcaagattg
    21061 ttcaagtaat cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc ttaccagata
    21121 tcaacaacgc attagttgtt tataaaaatg acgaaaataa atcaaaagtt gttcttgaag
    21181 cagcgttaga attaggggac ggagtgatcc ggaccattgc gatggaatca acagatggtt
    21241 tacaaagagg aatggaagtc attgatacag gcaaagcaat ctctgttcct gtaggtaaag
    21301 aaacattagg tcgcgtattc aacgtactag gagatacgat cgatttagaa acacctttcc
    21361 cagaagatgc ggaaagaagc gaaattcata aaaaagcacc agcctttgac gaattaagta
    21421 caagtacaga aattttggaa acagggatca aagttatcga tttgcttgcc ccatatttaa
    21481 aaggtgggaa agtcggacta ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag
    21541 aactgatcca taatatcgcc caagaacatg gtggtatttc tgtatttact ggtgtaggtg
    21601 aacgtactcg tgaaggtaat gacttgtatt atgaaatgaa agattcagga gttatcgaaa
    21661 aaacagccat ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt
    21721 tgactggttt gacgattgcg gaatatttcc gtgatgtaga aggtcaagat gtactgttgt
    21781 ttatcgacaa cattttccgt ttcactcaag ctggatctga agtatcagcc ttgttaggac
    21841 ggatgccttc tgcggttggt tatcaaccaa cattggcaac agaaatgggt caattgcaag
    21901 aacgtatcac atctacgaaa aaaggttcga tcacatcgat ccaagcaatc tatgtgccag
    21961 ccgatgacta cactgacccg gcgccggcaa cagcattcgc ccatttggat gcgacaacca
    22021 acttggaacg taaattaaca gaacaaggga tttatccagc ggtagatcca ttggcttctt
    22081 cttcaagtgc tttagcacct gaaatcgtgg gagaagaaca ttataaagta gcaacagaag
    22141 tccaacacgt attgcaacga tatcgtgaat tacaagatat cattgctatc ttaggtatgg
    22201 atgaattatc cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc caattcttct
    22261 tgtcacaaaa ctttaacgtg gctgaacaat ttactggtct tccaggttca tatgtccctg
    22321 tagaagaaac agtgaaagga ttccgcgaaa tcttggaagg caaatacgat gatcttcctg
    22381 aagaagcttt ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag
    22441 gctactagaa aggggtgccc catggatcaa tatttaacag taaatgtggt aactcctgcc
    22501 ggtttggtct atgatcacca tgcagctatc gtcgttgcac gtacgacagc tggtgagctt
    22561 gggattttgc ctaaacatgc accaatcatc gttccattga cgattgatga agttcgtgta
    22621 aaacgaacag attcagatac ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa
    22681 gttcgtgata atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat
    22741 gtatctcgtg cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca
    22801 aaacaagata cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt
    22861 atcaaagtat ctaagcactc ataaaatgag atgcggatgt ttatccgtgt ttaagaacag
    22921 taaaaagagg ttatgacaga agtggtc
//
LOCUS       DACENJ010000023        22468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.023, whole genome shotgun sequence.
ACCESSION   DACENJ010000023 DACENJ010000000
VERSION     DACENJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.023"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(34..753)
                     /locus_tag="IW748_000675"
     CDS             complement(34..753)
                     /locus_tag="IW748_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1103604.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            1090..1422
                     /locus_tag="IW748_000676"
     CDS             1090..1422
                     /locus_tag="IW748_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103605.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            1409..3412
                     /locus_tag="IW748_000677"
     CDS             1409..3412
                     /locus_tag="IW748_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1103606.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGVYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            3431..3901
                     /locus_tag="IW748_000678"
     CDS             3431..3901
                     /locus_tag="IW748_000678"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1103607.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            3922..4506
                     /locus_tag="IW748_000679"
     CDS             3922..4506
                     /locus_tag="IW748_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1103608.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            4521..5507
                     /locus_tag="IW748_000680"
     CDS             4521..5507
                     /locus_tag="IW748_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1103609.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            5497..5808
                     /locus_tag="IW748_000681"
     CDS             5497..5808
                     /locus_tag="IW748_000681"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1103610.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            5830..7611
                     /locus_tag="IW748_000682"
     CDS             5830..7611
                     /locus_tag="IW748_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1103611.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            7604..8980
                     /locus_tag="IW748_000683"
     CDS             7604..8980
                     /locus_tag="IW748_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1103612.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            8987..9622
                     /locus_tag="IW748_000684"
     CDS             8987..9622
                     /locus_tag="IW748_000684"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1103613.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            9652..11007
                     /locus_tag="IW748_000685"
     CDS             9652..11007
                     /locus_tag="IW748_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1103614.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKFAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(11199..12593)
                     /locus_tag="IW748_000686"
     CDS             complement(11199..12593)
                     /locus_tag="IW748_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1103615.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            12685..13656
                     /locus_tag="IW748_000687"
     CDS             12685..13656
                     /locus_tag="IW748_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1103616.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            13653..14434
                     /locus_tag="IW748_000688"
                     /pseudo
     CDS             13653..14434
                     /locus_tag="IW748_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(14603..14974)
                     /locus_tag="IW748_000689"
     CDS             complement(14603..14974)
                     /locus_tag="IW748_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1103617.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            15108..15551
                     /locus_tag="IW748_000690"
     CDS             15108..15551
                     /locus_tag="IW748_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1103618.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(15594..16403)
                     /gene="yidA"
                     /locus_tag="IW748_000691"
     CDS             complement(15594..16403)
                     /gene="yidA"
                     /locus_tag="IW748_000691"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1103619.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16422..17789)
                     /locus_tag="IW748_000692"
     CDS             complement(16422..17789)
                     /locus_tag="IW748_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1103620.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(17773..18603)
                     /locus_tag="IW748_000693"
     CDS             complement(17773..18603)
                     /locus_tag="IW748_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1103621.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            18766..19203
                     /locus_tag="IW748_000694"
     CDS             18766..19203
                     /locus_tag="IW748_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1103622.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            19279..19899
                     /locus_tag="IW748_000695"
     CDS             19279..19899
                     /locus_tag="IW748_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1103623.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            20088..20594
                     /locus_tag="IW748_000696"
     CDS             20088..20594
                     /locus_tag="IW748_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1103624.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            20611..21510
                     /locus_tag="IW748_000697"
     CDS             20611..21510
                     /locus_tag="IW748_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1103625.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            21597..22468
                     /locus_tag="IW748_000698"
                     /pseudo
     CDS             21597..22468
                     /locus_tag="IW748_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
BASE COUNT         7270 a         3906 c         4833 g         6459 t
ORIGIN      
        1 aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa agaaatcgaa
       61 taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt ggacaccaag
      121 gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt tggaaaaggc
      181 gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg aattcacaaa
      241 gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga cttcgtgagt
      301 tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg gatataatga
      361 aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc ctcggcagac
      421 tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt cgaattggtc
      481 tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa gttgcggact
      541 aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt gaggcgctga
      601 atctggagac atgactggca gaataagagg taatccttga gcatctaaaa ctgcatccac
      661 aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg ctcgaatcag
      721 ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag tagactaatt
      781 gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact tatctgttct
      841 tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat agttgaaaat
      901 attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc gtaagcttca
      961 aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg cctatactaa
     1021 gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc ttttttaaat
     1081 cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc agaagaagaa
     1141 aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga agaaaaagag
     1201 caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt ggtagaagaa
     1261 aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa agcagaagca
     1321 gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca agcaatcggt
     1381 gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa aagtgaccct
     1441 catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa tccaaaatgt
     1501 tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa aatattttcc
     1561 agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc ggctgaaaga
     1621 catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa agaaactcca
     1681 tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg aagaaaattt
     1741 catgaataaa ttagaagagg tccttgattt aaaagaaaag tgggaacagc taactgaaaa
     1801 acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc tggatattgc
     1861 tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga gtgcagcaaa
     1921 ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg aagaaataga
     1981 ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag ttgctgctat
     2041 agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg aacaaaatcc
     2101 taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc agcaaaaaaa
     2161 gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat gggtagaaga
     2221 agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc atgccactta
     2281 tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga tgcatgtgtt
     2341 aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa actcagaaga
     2401 gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac cttttgaatt
     2461 gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc cttggatgac
     2521 accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg gattgctgat
     2581 gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga tgaaacgttt
     2641 cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga tctacagttc
     2701 atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt tcccaatcct
     2761 atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg gtctgataca
     2821 gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag attacttgag
     2881 tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta ttgcattgat
     2941 tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc tatcgatcat
     3001 tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag tcaagggtgc
     3061 tgccaaagga gtgtataatc tttacggatt gactggttat atcggtgatt tagtcagtta
     3121 tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat tcaatatgct
     3181 agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga tcatcgcttt
     3241 acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg cacgattgca
     3301 gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg acccgctgaa
     3361 aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat aattggagga
     3421 tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat ttgcagtatt
     3481 agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg ttggaatgac
     3541 aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac aggctttgat
     3601 tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct ttttgatttt
     3661 tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag gcgcttctct
     3721 gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg cagcagcagg
     3781 tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat
     3841 ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat tgaatgccta
     3901 agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca gatgaatgaa
     3961 gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat cgatcaaaac
     4021 tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga aaaacaaatc
     4081 gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga agtacgacaa
     4141 gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc aatagctgaa
     4201 atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt gtattcatta
     4261 cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta tctttctcag
     4321 acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag tgatgaaact
     4381 gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa ttttcttttc
     4441 agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa tcaattattc
     4501 gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt cgcggaagag
     4561 agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat tctgtcgaag
     4621 cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac ggatatgaat
     4681 atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg ttaaaggaat
     4741 cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc cataatctga
     4801 aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat atcgacgatg
     4861 gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca aatgaactcc
     4921 ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag tctaccattc
     4981 ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga cggcttgccg
     5041 ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac ttgaccaata
     5101 tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg acgacggtac
     5161 tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat aaagatatgg
     5221 aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc cctgtcatcc
     5281 gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat ctaagttcat
     5341 tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc tttttgaacg
     5401 caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga agccatttcg
     5461 aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat cggtgtagtt
     5521 ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg ttatggtaca
     5581 accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt
     5641 tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta caaggaacaa
     5701 atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat agggctggaa
     5761 gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta tgaaaaggag
     5821 atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt aatggctgag
     5881 aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg agttatcgga
     5941 gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga gacttctgga
     6001 attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga attaggacca
     6061 gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt tatggaaatc
     6121 acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca tgagaaaaaa
     6181 tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga tgttatcggg
     6241 atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg gataaaaggt
     6301 acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg tgtcattgaa
     6361 accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg
     6421 ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg agtgattgat
     6481 acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt tggtgcaggg
     6541 aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt tgtctatgtg
     6601 ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc tgaattgatt
     6661 gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa tacatcaaac
     6721 atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc ggaatatttc
     6781 cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg ggcagaagca
     6841 cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta tccagcttat
     6901 cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc attaggatca
     6961 gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat cgccgtctgg aggggatatt
     7021 tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg tcttgattcc
     7081 agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta ttctctttat
     7141 tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc atccatggtc
     7201 accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt gcgtttggta
     7261 gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa atccattcgt
     7321 gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc aagagagaaa
     7381 caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg
     7441 ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga aaggatcgga
     7501 cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat tggtgaaatc
     7561 aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta aagaatatcg
     7621 tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg gtgtcaaata
     7681 tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga
     7741 agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta tcaatttacg
     7801 tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg aagacatgat
     7861 cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga tcctgcctga
     7921 aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt atccagatga
     7981 gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc gtgggcaaaa
     8041 attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg
     8101 acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg ctattggaat
     8161 cacttttgaa gaagcagaat ttttcatgga agattttcgt caaacgggcg caatcgaccg
     8221 ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gagcgtatcg ctactcctcg
     8281 tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg ttttggttat
     8341 catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag cacggcgaga
     8401 agttccagga agaagaggat atcctgggta tctttacacg aacttagcta ctttattcga
     8461 acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga ttctaacaat
     8521 gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta cagaaggaca
     8581 gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg atgtactccc
     8641 gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag aagaccatgc
     8701 agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa aagaattggc
     8761 cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga agtttgctga
     8821 gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga ttactgaaac
     8881 acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa aacgaatcaa
     8941 agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg cacgattgaa
     9001 tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa ctgccacaag
     9061 agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca tcctgttgat
     9121 tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag caatgtctga
     9181 ttttgtattg gcaaatgctt cgatcacgga gcctttcatc gaagaattat ttgttttgcc
     9241 ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt
     9301 gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg ggtatttgaa
     9361 ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc caaaactttt
     9421 ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg aaaagacacg
     9481 tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa cgatctatta
     9541 tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa
     9601 aaatatgggt accactgact aagtggtatc cttttttaaa agaggaaaac gatgattaaa
     9661 caaagcaaac aaataaagaa acgcttgccg gctgttcagc ttctcgcatt aggatttttt
     9721 ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa tagtgggcat
     9781 gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt gacgggattg
     9841 acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat catgcttttg
     9901 attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc attgataaag
     9961 aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct agaagaagta
    10021 tcaggtgtga tgaagctcat gctttacatc ctgaaatttg cctttgtgat ccaactgatt
    10081 ggtacggttg ctttatcttt tgtattcgtg cctgaatttg gctggttgaa aggtatctgg
    10141 tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt gttaggagac
    10201 agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc tttgatcatt
    10261 tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca gaaaaagaag
    10321 ataacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt aggcggattc
    10381 gttgtgttct ttttcacaga gcgcaatggg gaaacgctag tggaagggaa tatgtttcaa
    10441 cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata ttattcagtt
    10501 gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat gtacatcggt
    10561 ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt gttgctgatc
    10621 cagatgcgtt cgatgcttaa aggacgtacg agagcggaag catttggccg taccatccga
    10681 caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt gtgtgtagtc
    10741 gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat cgaatatatt
    10801 gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt gacgccagac
    10861 ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg agttggtgta
    10921 ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat
    10981 ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta gtgatttagt
    11041 aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc aaaatgttcg
    11101 tttatttttt gaggcactat ccgttgttga acctcgggta gtgcctcttt cttattgaaa
    11161 ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata tttttttatt
    11221 ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga ggatagggaa
    11281 attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg
    11341 ataccgttag caagaaagtc attctcaatt atcttaggat caatctcagg atcaactgga
    11401 agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa
    11461 agctgattgg ctcttttcgt aagacttaat ttagtttcta agatcctatt tgcttcattt
    11521 gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac atttatatta
    11581 aaaattgctt gttctatctt gttttggaca tgtactggaa ctacaagata agcaactcgc
    11641 aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt aggtaataat
    11701 ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga atggatatca
    11761 tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc tcgtcttttg
    11821 tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac accatgaatc
    11881 ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc gtcaaaatca
    11941 attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc gtatgtatat
    12001 cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat agccaatcca
    12061 ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc ttgagttttt
    12121 aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg gtaattaagc
    12181 aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac gaatttatta
    12241 cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac ttcattggta
    12301 gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc aatattcgta
    12361 tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt aaaatttaaa
    12421 tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg taatttattc
    12481 tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg cttcaatttt
    12541 tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg cattttctac
    12601 actccttaaa attgtatacc atacaatttt accattgatt gtattgattt tagaagttaa
    12661 tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt agtagcttct
    12721 tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt
    12781 aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaaga caaaaatatg
    12841 tttgcgatga tgcctgctta tttgggggag aacagatatt ttggaacaaa agtcattacc
    12901 gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat tttcttgttt
    12961 gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac ctggattcga
    13021 acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc agaaacactt
    13081 gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc taccattcgt
    13141 cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac ttttatcgct
    13201 aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt agaagatgca
    13261 gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt tcttcaaaaa
    13321 gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc tgatacaaga
    13381 gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta ctcagctacc
    13441 ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga ggaatcttgt
    13501 attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa aggagaatcg
    13561 aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg tgcagaatat
    13621 atttataaca aattagagaa aggggcttta aaatgaactt agattattac gtagttgatg
    13681 catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc
    13741 tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa acagcattta
    13801 cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga gaaatcgatc
    13861 tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac aaaatacctg
    13921 atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga attaaagatt
    13981 attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg actgaatatg
    14041 aagaagcaat tggggctaag attaaagaag cctacttagc acgtgattta tttttcgtat
    14101 tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc aaaaattttg
    14161 aattaggaat agagtcatcg tgacagcaga aagtgtgcaa aaagactttg tttctagaac
    14221 cttctttcca aaattaacaa tcaatgaaga tccagtatgt ggatcagcac attctaatct
    14281 tattccttat tgggcagaaa gaatgaataa aacaaaattg tcagcttatc aaatgtcatc
    14341 aaggggcggg gatctagaat gtgaattaaa agaagatcgt gtgataatag gaggaaaagc
    14401 agttttgttt tctaaaggta cctcgtttat ttgatttctt tcaaatgaat taaaaataaa
    14461 ttggtggaga gtggcagcct tatagctaca atggttgtta aaaaaagacg caaacttttt
    14521 caatttaatg aaaagtttgc gtcttttttt aattattggt gtttgttttt tattcaatca
    14581 agagtaaaat gccccatact aactatcctc ggatgacttc gattttataa ccatcagggt
    14641 caatgacaaa gtagtaagaa ggcgtagtgc caggtaatcc ttttaagtca gtaatctcaa
    14701 gacctgcagc tttatgttgc tcgtgtaatt tttctaaatc atcagctgca atagcgatat
    14761 gaccatagcc gtctcctaga tcatacgcac catgatcata gttgtaagtc aactctaatt
    14821 catagtcatc tccaggaagt gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac
    14881 ggcggcggct ttcttcaaaa ccaaatgcct tttgataaaa ttcgattgat gcttcaagat
    14941 ctttcacccg aacacatgtg tgagccattt tcataaattc atctctcctt ttccttttac
    15001 tcaatcataa caaaaagaag taagaaaata gttgatttgt gcttgttcat gagaaatttc
    15061 tcatgtaaaa tggatagaaa ataatactgt taaggtggaa acaattcatg gataaaccaa
    15121 tttttggtga aaaaaagcaa tctgctaatt atcaaacccg ttatgcagca tatattgtca
    15181 tagaaagaga caagcggatt gcggtgatcg aggctccaaa tggtgctttt ttccttcctg
    15241 gtggggaaat cgaaggagaa gagacgaaag aagaagcgat tgtccgtgag ctgatagaag
    15301 aaatgggaat tgtggcagaa atttcttctt atctgggaga agctgatgag tatttttact
    15361 cgaattatcg tgcaacttat tattataatc caggatattt ctttgttgct gattcttgga
    15421 agcgtatagg agaaccaact gaaaagacca ataaaatttg gtgggtgagc cctcaagaag
    15481 cgcttgtgaa attgaaacgt ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa
    15541 ataaaaactg aaagaaagcg agtagatcag ctttctctca gtttttttat ttattacagt
    15601 acatattttt tgattgcttc agctacaccg tgctcgtcgt ttgatgaagt gatgacattt
    15661 gctgcttctt tgacaagaga aacggcattt cccattgcta ctcctagacc agcatattca
    15721 atcatggata agtcgttctc gttatctccg atagccatga tttcatcttg atcaatacca
    15781 aggtgctgtg cgagattcgc aactgccgca cctttacttg cttctttatt tagtacttcg
    15841 aagtaatagg gcgcactttt gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc
    15901 gcaattgctg catctaaaat ttctggttcg tcgatcatca tcattttgac gatgttcatg
    15961 tctggtgtca tttcttcagg tgttcgatat tttagcggca tcttgactaa acttgcttcg
    16021 tggatcgtat aagcgctgat atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa
    16081 tggagatgac tgccgacacg gcgtgccatc acttcgatct ctaggaaatc ttcatgagtc
    16141 agtccgtgac gggcgatgat tttacctgat ttcgtttgtt gtacgagtgc cccgttataa
    16201 gtaataacgt aatcattatc ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct
    16261 ggtaagggac ggccagtgca taaaacgatc gaaacacctt ttgcaacagc agcggcaatc
    16321 gcttctttta cttcttttgt tacttctctt ttctcattta ataaagtacc atcgatgtcg
    16381 atagcgacta gtttgataga cataatttcc tccaatatat tttaatttat ttcaatcaat
    16441 gcgccattac ggatatggct ggcaaattct tgataagttt cgtcaaataa atcataatga
    16501 tcacgcaagg agggatcaac catttctttt ggaaagaaaa atcgctcatc tccttgttct
    16561 tgtcccgcta atgcagcaac aagctgacta acctgtgata attctatcaa agttccgtca
    16621 tttcgcaaaa tctcgatttg tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa
    16681 ggcagatcgt agctagaatt cacagcagtg taataaacag ggttatagcc aaccttttct
    16741 actaacaaag tgagttcttg gactaatttt gaatcccggt tgccagaaaa agtagcagat
    16801 ttcagtggtt tacgattcaa aaaacgttta gctaaatcat ttaaaatagg atcagcagat
    16861 tcagaccatt gagtaaagta agtgcccaag accccatcat caagtttgag gtattcttct
    16921 aaagtaaagc ttccgtcaag aaatggcaga agtaattgag aatggagttc aaaactgctg
    16981 gcatcttctt gatacaactc gtgtgcgcga tgaagcagat gctccaaaat cacttccata
    17041 ccacgggaaa caggatggaa gtacacttgc acatacattt gatatcggct aacgatataa
    17101 tcttccactg catgcatgcc actcatggaa aaagcgatcc cgccttcata aggacgaatc
    17161 acacgtaata ttcgggtaag atcaaatgtt ccatattctg ttccagtgaa ataagcatcc
    17221 cgaagcaaat agtccattcg atctgcatcg atctgactag aaatcatttg gacaacttgt
    17281 gggttggggt aagttttttg gatcacacta gctacctttt caggaaaacc ttcttctact
    17341 cggttaagaa tctgatacac ttctgtttct ggagaagtga tgatctctac agtgatggct
    17401 tcatgattcg tatggaagat atgttcaaat gtatgagagt aaggaccatg accgacatca
    17461 tgcaacagtg ccgcacataa agtcaccagc cgttcctgat catcccagcc tccgttgcct
    17521 attttttctt ttgaaaaatt tcgagaaaag atatcgcaga ttcgtcttga aatctcataa
    17581 acgcctaatg aatgggtaaa acggctatgt tctgctccgt gaaaagtgaa agaagaagtg
    17641 cctaattgtt taatgcgtcg taaacgttgg acttctttcg aattgatcaa atctaagatt
    17701 actttatgct ggacatggat atagttatgg actggatcac gaaaaacttt ttccataggc
    17761 agcatttgat atttataagg ggttgtcata atgtagctcc tttatgttat tacgttgttc
    17821 cattctctgg atttggttgt tccattgctc cagctggatt tcttttttta gccagtcggt
    17881 agaatcctgt aaatcacttg cagaatacag cttttcaaaa gcttttgcaa atgcttcttt
    17941 gacttctttt acaaaaaggg gttggtcaag cagttcttca agtgttgcca ttgtagcagg
    18001 atccactgca ggatagccat ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg
    18061 cttcatgact tgcccgcgtt tttcctgatt accgttgaca cttaagtaca tcataacaga
    18121 aatcccattt ttgatccgac gctgagcgat accggcaaat tttttgccat ggatgcttaa
    18181 atcataagtt ccaggacaat aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg
    18241 aaaagcgtat ttagtcaata ggagcatttg ctcgtaagcg tcatctatgg ttattttttt
    18301 tgtgtcactt tgagggaaaa tcaaagaaat atttaaaatt ccttcatctg atacgatccc
    18361 taatccacct gaatttctga tcactggagc gtaacctgca tctttcagtg tttggatccc
    18421 gtcacttaga aaaggagttc gaacatcctt catacctaag atgactgtgc agtcatattg
    18481 ccagaaatgt aaaaagattt gctggtgttc tttagaatat tccgttaata tatctgttaa
    18541 tgcaaaaggg gaaaagtcgt ttttttgata aatgtgctga tctagtcgaa tcgcagggtt
    18601 cattcaaccg gctcctttgt ataaattcat attttctaca atattatact ctaaaatcaa
    18661 agagttgttt tggaaaaaag gttttctgta aaaatggaca agttggttga caaaacaaaa
    18721 gaaactagga acaatgtagc tatgagaagt aaaggagaat gaataatgga attatcacaa
    18781 ggaacaccga tttcaatcaa attgcgtaca aaagtaaaac agcaagggga agtgcaagat
    18841 ttttattttg atttgaaagg gcaaatggtg acaattggcg atacgctgta catacgttat
    18901 aaagagattc aggaggagac gggcgatgag attcctgtaa cgatcaaatt agttccagat
    18961 ggtcatgtac agctgatccg tgcaggagaa atgcggatgc gtttgaaatt tggctataaa
    19021 gaacggttag aaacatccta tcgaacacca tacggtatga tccagattgc tacttttacg
    19081 aaagaactgc atgttagttt gaaagaccgt cccacagcag ggaaggttcg aatcgattat
    19141 gatctgttca tgggaccgga aaaaatcggc gaatattatt tgaccttgga ttttacagcg
    19201 taaatataaa agaaaagttt gattttttgt gtctgatttt gtatgataag gagtagactg
    19261 tgaaaggacg tgtcctgatt ggaaattagt gtatttgaag gtgcaaacaa aagcgaatta
    19321 tcaatgatcg aagtagcaca cgcgatctta gaacaacgtg gcgatgtcat ggatttttct
    19381 gatttagcta accagatcca aaactatctt gagaaatcag acagtgaaat tcgtgattca
    19441 ctcgcacaat tttatactga cttgaatatt gacggcagct ttatctcatt aggggataat
    19501 cgttggggct tgcgttcatg gtatgctatc gattcgatcg atgaagaagt aaaccatgga
    19561 atggacgaag aggatgaaga tactccacgt cgccgtaaac gcaagaaagt caacgccttt
    19621 atcaatgatg atgaagacgc gatcgattac aatgacgatg acccagaaga tacagatctg
    19681 acagaagaag atgacgatga tctgtttgat gacgatgatg atgaagatga agaaatcgct
    19741 gcttataatt ctgacttgca agaaatcgga gcagatgacc cgacagatga tgaagaagac
    19801 ttgccaggaa atatcgaaga agatttaagt atcatagacg aagacgatga tgatgaagat
    19861 tatgaagagg aagaattttc tgactctgaa aaagaataaa cattattttc ttacgagagg
    19921 ttgtgacaga gacgatcagc ctatcagaat aaggaggtgt gacacgattt ttgtcacacc
    19981 ttcttttttg tatataaaaa tcccaagtaa atacataaaa aataaaaaaa ttgaaacttt
    20041 tagcgcacct tgtacgtcat attaagtgag gcagaaagga gtggagaatg atttttactg
    20101 gatataagaa aaaacggcag atcgaaaaat accgtcacct gctgaatgga ctcatcgaaa
    20161 ctgattatca aaaaatgttc cgtatcacga tgaattacgt acatgataaa gaagaggcct
    20221 tagatgtgat gcaagatagt tttcataaag ccttgaacta ttttgaaaaa gaaaaagaga
    20281 tcgagcattt tagtgcatgg ttttatcgga tattgatccg cacggcatta gatagttggc
    20341 gcagacaaaa aaggacgcct gtggacttat ttgaaattca agatatcccc caacagttac
    20401 ctttagaaac ttccgttgca gagctccata tgattttaga taaaatagac agtccggaaa
    20461 aagagattct gatcctctac ttttttgaag gatttcgttt gaaagaaatt gcaactatct
    20521 tggatatgaa tgaaaacaca gttaaaacga aaatgtaccg ctcattgaat gctttacgtc
    20581 aaatcctaga atgaaggaga gaagagtgcc atgtattccc aaaaagaaaa tttaaaaaaa
    20641 ctaaagaaaa cttataaaga acaagcaatt cccaaagggc ttaaagagga gatccgccag
    20701 cgttttatta tcgaagagca atcatttata aaagcaaggc ggagaaagag acgtctccaa
    20761 gggttcggag tatctttggc tatcgtaatg attacaacaa gtagccttct ttttaatagt
    20821 caagttcgaa gctttgcaga agatttaccc atcttaggat cagtcattga attgatctta
    20881 ggcgaacgct ttactgatcg atctgaaaaa atcgatattc aggtaccgaa gatcaataca
    20941 caaaatgaga aagaaaataa aacaattcat ggattaaatc aaaaatactt tcgcgaagga
    21001 caagcagatt ttgaaaaagc tgaaaaagaa tatggaaatt ttgaaacgga tcattaccaa
    21061 gttttgggag attatcagaa aatcgtagac gataaccggt ttctcgtgat tgaaaggcag
    21121 atcacccaaa cagcagcaga tagccatgtt gaaaaaagat acgacacaat cgacaagaaa
    21181 aacagtgtcc agctctcact tccactttta ttcaaagatg acacttatct atctgttttg
    21241 acaaaagaag tcaaacggca gatggccgaa caagtcaagg aagatccatc taagtactat
    21301 tggactgagc aagatattca agaagggacg atagaaaaac caacacttgt tacgcctaca
    21361 cgaagttttt atttgaacaa agagcatcaa ttagttttga ctttttctca atacgaaatt
    21421 gctcctggtt atatgggtac accagaattt gtcattccta agtccgtgac aaaaacgatt
    21481 ttggcatcgg aagattattt ggatcattag acgagaagta gttgcgacta gcagtttcaa
    21541 ttttcaagta agtacaagta aataataaga agagaaaaag agaaagaaga gatatagtga
    21601 aaagaaatta tttagtcatc agtattattg ctttatccgt gcttttgttt ttcgaaatta
    21661 cttcggtggc ctccttgatc accgaagtaa aaggacaaac acactcttcg gagcattcgt
    21721 cacaatcttt caaacaatca gaacaatcaa ccattaaaag ccaatcaact gaaacagcta
    21781 acgtcaaaga agccattcaa gaatcagcgg aaaaggtaca aagtcaatca tcacaatcaa
    21841 cacaagacag tacagaagca atccaagacc cgactgtcaa aagcgtcgca atcagctttg
    21901 atgatggacc aggagcgacc acaacgccgc agcttctgcg aatcttaaaa gaaaaaaacg
    21961 tgcatgtcac ttttttcgtc ttaggagaaa atacggcgca gcatcctgaa atcgtgaagc
    22021 agacagctga agcagggcac gagattggca atcacacata cgatcatcag aatctggcca
    22081 ctctttctgc ccagtcgatg acggaggaag tgacgaaagc agacactgag atcaaaaaaa
    22141 gtcactggca aaacccccgc ttttgttcgt ccgccctatg gttctgtcac gaatgtagga
    22201 gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca
    22261 aggaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg gtcaattatt
    22321 ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg
    22381 aaagagcaag gctatcgcat cacgacagtt agcgatttat tagggcatcc aacggctgtc
    22441 gagaactatt atggcagaaa cgatcatc
//
LOCUS       DACENJ010000024        21059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.024, whole genome shotgun sequence.
ACCESSION   DACENJ010000024 DACENJ010000000
VERSION     DACENJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.024"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            187..2073
                     /locus_tag="IW748_000699"
     CDS             187..2073
                     /locus_tag="IW748_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1103626.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2085..3032
                     /locus_tag="IW748_000700"
     CDS             2085..3032
                     /locus_tag="IW748_000700"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1103627.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3052..3579
                     /locus_tag="IW748_000701"
     CDS             3052..3579
                     /locus_tag="IW748_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1103628.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3642..4295)
                     /locus_tag="IW748_000702"
     CDS             complement(3642..4295)
                     /locus_tag="IW748_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1103629.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4427..5269
                     /locus_tag="IW748_000703"
     CDS             4427..5269
                     /locus_tag="IW748_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1103630.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5427..6323
                     /locus_tag="IW748_000704"
     CDS             5427..6323
                     /locus_tag="IW748_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1103631.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6326..7039
                     /locus_tag="IW748_000705"
     CDS             6326..7039
                     /locus_tag="IW748_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1103632.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            7059..7577
                     /gene="msrA"
                     /locus_tag="IW748_000706"
     CDS             7059..7577
                     /gene="msrA"
                     /locus_tag="IW748_000706"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1103633.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7574..7801
                     /locus_tag="IW748_000707"
     CDS             7574..7801
                     /locus_tag="IW748_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1103634.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            7953..8504
                     /gene="lepB"
                     /locus_tag="IW748_000708"
     CDS             7953..8504
                     /gene="lepB"
                     /locus_tag="IW748_000708"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1103635.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8587..9129)
                     /locus_tag="IW748_000709"
     CDS             complement(8587..9129)
                     /locus_tag="IW748_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1103636.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(9366..9764)
                     /locus_tag="IW748_000710"
     CDS             complement(9366..9764)
                     /locus_tag="IW748_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1103637.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            10024..10884
                     /gene="ylqF"
                     /locus_tag="IW748_000711"
     CDS             10024..10884
                     /gene="ylqF"
                     /locus_tag="IW748_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1103638.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            10877..11644
                     /locus_tag="IW748_000712"
     CDS             10877..11644
                     /locus_tag="IW748_000712"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1103639.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            11700..12557
                     /gene="dprA"
                     /locus_tag="IW748_000713"
     CDS             11700..12557
                     /gene="dprA"
                     /locus_tag="IW748_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1103640.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            12679..14757
                     /gene="topA"
                     /locus_tag="IW748_000714"
     CDS             12679..14757
                     /gene="topA"
                     /locus_tag="IW748_000714"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1103641.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            14726..16102
                     /gene="trmFO"
                     /locus_tag="IW748_000715"
     CDS             14726..16102
                     /gene="trmFO"
                     /locus_tag="IW748_000715"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1103642.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            16300..17205
                     /gene="xerC"
                     /locus_tag="IW748_000716"
     CDS             16300..17205
                     /gene="xerC"
                     /locus_tag="IW748_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1103643.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            17242..17790
                     /gene="hslV"
                     /locus_tag="IW748_000717"
     CDS             17242..17790
                     /gene="hslV"
                     /locus_tag="IW748_000717"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1103644.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            17804..19204
                     /gene="hslU"
                     /locus_tag="IW748_000718"
     CDS             17804..19204
                     /gene="hslU"
                     /locus_tag="IW748_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1103645.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            19222..20013
                     /gene="codY"
                     /locus_tag="IW748_000719"
     CDS             19222..20013
                     /gene="codY"
                     /locus_tag="IW748_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1103646.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            20095..20970
                     /locus_tag="IW748_000720"
     CDS             20095..20970
                     /locus_tag="IW748_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1103647.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         7410 a         3671 c         4419 g         5559 t
ORIGIN      
        1 tcaaaaaaaa cgagtccaat aaaataaaaa ggtaaaaatt ttcaagtttt ataaaaaaca
       61 aaacatatca gaacaggatc gaatcggtcc tgttcttttt ttgagaacac aaaaagaaca
      121 aataccaaaa aaattttcct gaagttcact atttttatga tacaatatcc aagataagga
      181 gggaacatgt tgaaagaatt aaaagtaact gacctcaaaa aaacttatgg tgaaaaagat
      241 ttgtttgacc agatttcttt tttggtccac gaaaaagacc gtatcgggtt gattgggacg
      301 aacgggacgg gtaaaacaag tctcttaaat attattgccg gtatcgatag cagcgacggt
      361 gatcggcaaa cagtgtttta tccaactgat taccgtattg gttatctttc tcaagcagct
      421 gaattttccg atgaattaac tgttctccaa gcagtctttc aaggaaacag ccccttgatc
      481 caaactgttc gagcatatga ggaagcattg atcgaactag gggaaaacgg agaagaccct
      541 gatgtgcaaa aacgttatgc taaagcagaa gaacaaatga ataaagaaga tgcttggact
      601 acagatacca acgcaaagat tattttgcaa aaattaggaa tcagtgaatt agataaaaaa
      661 atcagtactt tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt gttgattgat
      721 gaacccgatt tactattact agacgaacca acaaaccatt tagattatga agcgatcgag
      781 tggttggaaa actatttgaa acaataccgt ggcgcactat tgatggttac ccacgatcgt
      841 tattttctag atagagtagc aaaccgaatc ttcgaacttt catttggaaa attatacgag
      901 tataaaggaa attatgaagc ttacgtctta gaaaaagcag aacgtgatcg tgtagaagta
      961 gaacaagaag aaaagcgcaa gcggctttat aaacaggaat tagcttggat gagggctggc
     1021 gtacaggcac gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga tctaaaggag
     1081 aacttgtacc aagtaaacca agaagatgat ttagagctga acctagcgac acaacgttta
     1141 gggaaaaaag tactggaaat caaagatggc agttaccgta taaatgacca aacactgcta
     1201 gaacatttag atttattgat tcaatctcgg gaaagactag gtattactgg taaaaatggt
     1261 gcgggaaaat cgacattgct taatattcta gctggcagaa ttcccctgga tagcggtaca
     1321 atgtctatcg gtgaaacggt tcatcttgcg tattatacac aagaaaatga agaaatggca
     1381 ccagataaac ggatgattgc ttacttacaa gaagctgctg aagaagcaaa aacagcagat
     1441 ggcagtcaaa tcggagtagc agaattactt gaacgatttt tatttccaag atttatgcat
     1501 gggacactga taggcaaact ttcaggcgga gaaaaacgcc ggttattttt gttgaaactg
     1561 ctgattcagc aacctaatgt tttattattg gatgaaccga ccaatgattt ggacatagca
     1621 acattgacga ttttggaaga ttattttaga agttttccag gtgctgtgat cactgtttcc
     1681 catgaccgtt atttcttgga caaggtagca gataagttat tagtattcca aggaaacgga
     1741 aaacaagaac tttattacgg aaacatgtcc agttatttac tgaagcaaaa agagacccag
     1801 cagcctgctg aaaaagcgaa accaaaaata cagagcaagg aacctgctga aaagaagaaa
     1861 ttatcttaca tggaacaaaa agaatgggaa acgattgaag atgagattgc agaacttgaa
     1921 gaaaaaatct cattgctcca agaagagatg aatcatcaag gagataattt cactcgatta
     1981 caagaattgc aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa aatggcacgg
     2041 tgggaatact tgagtgaatg ggtagaagat taggaggaaa aaggatggaa caagcgtatc
     2101 ttgacttagg aagaaaattg ttggaagaag ggcatcgtaa agaagacaga acaggcacag
     2161 gcacattaag cttattcgga taccaaatgc ggtttgatct gcaaaaaggg tttcctttgt
     2221 taacaacgaa acgagtacct tttggattga tcaaaagcga attgctttgg tttttaaaag
     2281 gagatacgaa tatccgctat ctattacaaa ataaaaacca tatttgggat gaatgggcat
     2341 ttgaacgcta catcaaaagt ccagactata atggaccaga tatgactgat tttggccata
     2401 gatgtttgac agatcctgaa ttcaatgaag aatataaaaa gcagagcaag cttttttgcg
     2461 agaagatcct aaccgatgat tcctttgctg aaacatacgg cgatcttgga cacatctacg
     2521 gctatcaatg gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa atcaaacaag
     2581 tgatcgaagc aatcaaaaag actcctgatt ctagaagatt gatcgtttct gcatggaatc
     2641 cggaagatgt tcctagcatg gctttgcctc cttgtcatac gatgtttcaa ttttatgttc
     2701 aagagggacg gctaagctgc caattgtatc aacgcagtgg ggatgtgttt ctaggcgttc
     2761 cgttcaacat tgcaagttat gcgttgctga ctcacctaat tgcacatgaa acaggtttag
     2821 cggttggcga atttgttcac acattaggag atgctcatct ttatatcaat catatcgaac
     2881 aaatgaaaga acaattgagc cgagaaatac gcgcatttcc gactttagtt ttgaatcagg
     2941 aaaagcagtc agtatttgat tttgagatgg aagatatcca attagaagga tatgacccac
     3001 atccggcgat caaagctcct atcgctgttt agaatcagaa aggatgagaa aatgttagct
     3061 gcaatttggg cacaggatga aaacggattg attggaaaag aagaccgtct tccttggcgt
     3121 ttgccaaatg atctgaaatt cttcaaacaa atgacagaag caaatacact agtgatggga
     3181 cgaaaaacat tcgaagggat gggtagccgt ccgttgccga atcgtcagac gattgttttg
     3241 acgagagatt cttcctataa agcagaaggc gtgcatgtca tgcatagtgt ggaagacgtg
     3301 cttgctctgg aaaaagaaac agacggtatc tttttcatcg ctggagggtc agctgtttat
     3361 caagaatttc tgccattttg tacgatatta taccgtacag tcatccatca tgtattcgat
     3421 ggagatgctt attttccacc tgttgactgg tctgattggt cactgatcaa tactagtcaa
     3481 ggagaaatgg atgaaaaaaa tcgttatccg catcaatttg aaacgtatca gcgtaatgag
     3541 aatcctgtaa aaagcgggac aggcgaattg acgatataaa aaagctatgg atagactagc
     3601 tgttatttag agctgtccca tccatagctt tatttttttt attaagtaaa gaataaaaca
     3661 gagaagtaca taaaacctgc acctaacata acaaacaaat gccaaacaac atgcatatag
     3721 cggatagaat tcaagctata aaagaatgcg cctatcgtgt aagaaagccc gcctgctacc
     3781 atcaaaataa caccagtcat tccaagagag tgataaagag gaacagcagc cacgacacag
     3841 agccacccca taaaaatata gatgatcgta gatacttttg atactctctc ccgtttatgc
     3901 aacgtcaacg ctttatatat aatgccaaga atcgcaaaca accagattaa tacaaataaa
     3961 gtccaaccta gccatcctcg gatactcagc aaacaaaaag gagtataact tcctgcaatc
     4021 aaaagaaaaa tcgaactatg atcgaatact tggaacacct tttttgcttt tgtaaaaatc
     4081 aatgaatgaa acaaagtaga cgttaaaaat aaaagaatca tcatcgatcc atagatagca
     4141 taagagacaa tatggaggga ggaatgcaag tgggcacctt ttactaaaag aaccactagt
     4201 cccgcgatac tcaatccagt cccaattcca tgggtgactg cattaaacac ttcatttaat
     4261 atttcatacg ttcgattaaa tttcgttttt tccatgaaaa cggctccttt actaggtgta
     4321 attaatgaaa aatcattatt tgtctgttat actaaaaata gaaattgtct tacatgtagt
     4381 ttaacatgaa taaggcatct tgttaaagaa agagagagag aaaataatga caaatgtaaa
     4441 aatcgtaaca gattcttcat gtacgatgga aaaaaatgta cgcgatgctt taggcattcg
     4501 tgtcatgccg ctatccatca tgatcgacgg aacagtatat cccgatgatg accaactaga
     4561 cggcgaaaga tttatggaaa tgatggcgaa agcaaaaaat ctgcccaaaa caagccaacc
     4621 accaattgga gaattcatct cgatgtatga tgagctagga gaagacggca gtgaagtgat
     4681 ttcgatccat atgacaaaag gcttgagtgg aacagtagaa gcagcaagac aagccagtaa
     4741 tctaacaaaa acgaaagtaa cggttatcga cagtgatacc actgaccaag gattatcttt
     4801 tcaggtaatt cgagcagctg aattagcaaa agaaggtaaa acagtagaga aaatccttga
     4861 agaagtcaaa aaagtacaag acaatacaaa attatacatc ggaatatcca cactagataa
     4921 cttggtaaaa ggtggaagaa tcagccgaac aactggtttg ctgtctagtg ttttgaacat
     4981 acgcgtggtg atgaatttcg cccattctga attaatccct gtaactaagg ggcgaggaaa
     5041 gaaaacgttc gataaatggt ttaataattt gaaaaatgaa ctgaaagaat tgccaaatgt
     5101 gcgacaaatc ggtatttcac atgcggatgc aaaagaattg gcgcaagaat tcaaagaggg
     5161 tttgcaagaa ttattcccgg acatgcatat ccctgtactg catacgaatc cggttatcgc
     5221 tacgcatact ggtaaagacg cgtttgcaat catgtattat gtagattagt cgaaaagagg
     5281 ataagccctc agcctgaaag gcttgcagct tattctcttt ttctttataa aattaggaaa
     5341 ataattgttt tcttttttca taaagactac ctttttgata aaatttttga taacattaga
     5401 accaatcgaa tacaagaggt gaaaaaatga aattaacgaa acgacatttg atcctcgttt
     5461 tgttcccatt attaattgcg atcatcatct atgcaggttt agctgcttct ataccgaaag
     5521 ctggcccact aatgaaacca gtaactactg tgagagaaga tccaaataaa aaagaaaaac
     5581 ttcactacat agctatcgga gattcgctga ctgaaggagt aggagatcaa accaagcaag
     5641 gaggattcgt tccccttgtc gcaaatgatc taaaagaccg ttataatttg accgcggttg
     5701 aaacagaaaa ctatggtgta aatggtgaac gaagtgacca gatcttaaag cgagtaaaaa
     5761 caaaagaaga aattcgaaac aatatcaaaa cagctgattt tatcacactg actgttggcg
     5821 gtaatgattt gatgaaagtt atccaaaata atttattcgg gttaacggtc aattccttta
     5881 aaaagccact gaaaaactat caggaaaatg tcacagaact attggaagaa tttcgatccc
     5941 tcaataaaac cgcacctatt tacattttag gcatctacaa tccattttat ttgaactttc
     6001 ctgagatcac tgacatgcag acaattgtta acaattggaa caaaggaaca gaaacggtga
     6061 caaaaaaaat gccgaactgc tactttatcc caatcaatga tttgctttat caaggattga
     6121 atcaggaagt aggtgtcaca gaagaaggaa cagatcaaac aactagcacg acagaaaatc
     6181 taaatggcat caaaaataat gtgctctatg aagaagatca tttccatcca aataatttag
     6241 gttatcaatt gatggcaaat gcagtgagag atcagctgat cgagacacaa aaccaatggt
     6301 tagagaagga ggatgatcaa taaacatgaa aacaagatct gacgtatctt caaacaaaac
     6361 aaagaaaaca aattactgga aattcgcctt ccttatcctt cttggggtag tacttggttc
     6421 gaccgtattt ttaggcagcc gtatttttgc taatcgggaa ccagaactcc cagaaatacc
     6481 agctcttacg gaaagacaag gagatcctgt cttaacgatc aattcaaata aagaaaaagt
     6541 caatcaaatc atcagctttt tccttagtga gtatcaaaaa gatagtgata ttgaatataa
     6601 attttatttg gaaaatgaag cactactcaa tggtactttc gaagtactag gttttcctat
     6661 cgatttctat ttatattttg atccgtatgt gatggaaaac ggaaatgtcc aactaaaagc
     6721 tagaagtctt tctatcggaa cattgagttt gccaatcaaa gatgtgatga atatgatcaa
     6781 gagaaactat aaattaccag attggatcga agtcaataca gatgatctga ctatcatgct
     6841 taggttggat caattccgaa tgcaaaacgg gatgtatatt aaagcagata aaatcgactt
     6901 agtaaatgat gacattcggt ttagtctata tttgccaaaa gatacctctg atgcttcaaa
     6961 caactcaaat accaacaaaa ctagcaccag cagtactgaa agcaagacaa aagaaaataa
     7021 aaaatcatcg gatgagtagt attagagggg gaatcataat ggaacgagca atatttgcag
     7081 gaggctgctt ctggtgtatg atccagcctt tcgacacgct tcctggtatc catacagtga
     7141 tgtcaggata tactggaggg catgtaccag atccgactta tgaacaagta aaaagccaaa
     7201 cgactggaca tacagaagca gtggagatta tctatgatcc aacattgatt tcatatgaac
     7261 aattagtaga actttactgg cagcaaactg atccaactga cgctttcgga caattcgagg
     7321 atcgcggtga caattatcgc ccggtgatct tttatttgac agaagaacag cggatcattg
     7381 ctgaaaaaag taagaaacag ttagctgaaa gtggacgttt cgaacagcca atcgttacaa
     7441 aaatcgaacc agcccaacca ttttatctag cagaagaaga ccatcaagag tattataaaa
     7501 agcatcctga aaattttaaa agaaaccatg cacgccgggc tgcctttatc gaagaaaatt
     7561 ggaggaacga agggtgaatc gaagcttttt tcattattta atgacactgc gaggcggtgg
     7621 cacacatgat gctttatctg ttttcgctac agaagccggt aaagacatcc aatttcctaa
     7681 gcacagctct agctacgaag aaatttctaa ttatctggaa ctgaatgtcg actatttgcc
     7741 gtccatggat atctttgacg aagcatggga aaaatattta gaaaataatc atgacatata
     7801 agagatagtt ttgtacttta tgatcaaaaa agttttttgt ccaatagcct aattgaaaga
     7861 attattttta tttcagctat ctagtcgcat ttttttgata tttttcttat aataaagaaa
     7921 gaatgaaaaa aagagggatg gattgataga gattgacaaa aaaacaacgc tatattaatc
     7981 gcttctggct tgtattcaaa tacttacttg tttctgtatt ccttgcgttc atgttgcggg
     8041 gatttttatt catcccggta ccagtggaag gcaattcaat ggaaaacgtg ttgaagcaag
     8101 gtgatatggt tgtgatggaa aaattttcgg agatcagacg atttgatatt gttgtttttc
     8161 aattagcaga tggcacgatt tatatcaaac gagtaatcgg tcttccagga gaaaatgtca
     8221 gctaccaaaa tgatcaattg aagatcaatg ggaaagtagt taaggaacct tatctaacta
     8281 aaaacctgaa atcagatcat gcaaatgcat cctatacgac ggactttaca ctacaagagc
     8341 taacgggaca atccaagctt ccggaagata attattttgt acttggagat aatcgaagag
     8401 tttcaaaaga tagtcgttcg tttggcacga tcaataaaac tgatattcta ggtaaagcaa
     8461 gatttgtcta ttatccactt gatgaaataa aatggatcca gtgagaaaca ataaagcgtg
     8521 cagtctgttg agactgcacg ctttattgtt taatccaaaa tcagtttact accttagtac
     8581 gtaaaatcaa acagctcgtt taaacacttt tctagcctct aaagttttga taaatgaata
     8641 aacaaccatt atttgaatag ggatttggat catattcctg atgaatctac ctagaaaaat
     8701 tactttgaaa tctgcaccaa acaaaacaga catcagccaa gaattgataa aaaggctgac
     8761 tgggatcata atcaataatt gggcagcgat gacggaaggc aggtttatct ttttatgata
     8821 taaaaataag ccattaataa atccaccaag gaaagccgtg ataagaaaag cagcttgaaa
     8881 cgttcctgaa ccaaatatcg tgaaatttaa agtgttcgac aacatttcaa gcaaaccagc
     8941 ccagatcggt ccatatagat aacccataag cgccatactt aaaaatccga atgtgatttg
     9001 aaaataagca ggaccaatcg ctatcttgta taagatgaca tcaagggcta ataaaagagc
     9061 agaggtcgac aacaatcttg cattcatttc gggcaatcta aaaacgtctt ttgcaatcaa
     9121 agtgttcaaa atattctctc ctttgtcagt taataataaa aggataccat aaaatcacca
     9181 atgtgtcatg acacattggt gatttacata cactggcatc taacaattaa aaaaagggtg
     9241 agaagactgc ttttataaac tgtaaaaaag agattgtacg aataaaaaaa atagaggcgg
     9301 agaatactta tcgtatcatc cgcctttgtt tttttgttat aaaaaacgtc agattagtta
     9361 tctgcttaca tcttcatcca tacgggttct aatgtatttc cgtctagatc ttggacttct
     9421 aatgaaaaca tttcttcttc aggcattccc atatctactt cgtggaaagt accaccattt
     9481 tcttttgcaa tttgagcaaa tttttttact tcatccgcat tttcaacact aaacgaaatc
     9541 aaagcaccac ttgttttttt attatcagct atttctttat cttttataaa ttttttataa
     9601 aaatcatgag agagaagcat tatccaaaaa gtgtcatccc ataccattga accagcttct
     9661 tcagtagaaa attcttcatt ttttttaaaa cccagctttt cataaaaagc aacggaacga
     9721 ttgacatcag caacaggaaa attaacaaaa accatactat tcatttgttt cgtctccttt
     9781 ctgttttgtt cgtctatcat ctcttattta ggaaacgctg tcaaaaattt agattcatat
     9841 atagaaacca aaataaaaat ttgcatcatc aaaaaagtga agatgtgata gaatgaggac
     9901 tggttcactt tcacttaact aatagatgga atttctttat aataaaaaag tgaatttata
     9961 ttgaaaaaga aaaaaacaag taaagccggg gaatattccg gtatgaaagg aatggagaga
    10021 acaatgacca tccaatggtt tccgggacat atggcaaaag cccgacgaga agtttctgaa
    10081 aaaatgaaat atgtggacat cgtatttgaa ttggtagacg cacgattacc tttatcttct
    10141 cgtaatccaa tgctagatca gatcattcag caaaaacctc gccttgtttt attgaataaa
    10201 gcggatctgg cagatccaca acagacaaag ctgtggcaag aatattttcg taaacaaggc
    10261 catcttgcat tagctatcac ggcacaagaa agtaaaggaa ttaaaggtct agtacctgcg
    10321 gctaaagaag cattgaaaga aaaacgggca agagatgctg ctcgggggat caagcctcgc
    10381 gctattcggg caatggtctt aggtattcca aatgttggga aatctacgtt gatgaatcga
    10441 ctagtaggca agaaaattgc acagacagga aataagcctg gcgtaacaaa aggtcagcag
    10501 tggcttcgtt taggtaatga gttggaattg ttagacacac cagggatttt atggcccaaa
    10561 tttgaagatg aagaaatcgg caaaaaacta gcgctgacag gcgcgatcaa agaccagctt
    10621 gtccatttag atgatttagc gatttacgga ttagactttt ttgcccgata ttattcagga
    10681 agaatcagcg atcgttatgg tttatcaatc gagcaagaag aaaaattggc accggaatta
    10741 ctgatggata ttacagcaaa acgtgggtat aaagaagact atgatcgtgg cagcgaatta
    10801 gtcgtgcatg atattcgtca aggaaaactt ggacgataca cactggatcg ttgcgaagaa
    10861 atggagcaaa aaagtgatga ctgaatccat ccaaagtatc aaagaacgat tgaaaagcat
    10921 acagagaata gatgatccta cactagaatc attaagaaat gatccacgca aaggcgtgca
    10981 gcaagcactg gcttcttttg aacgacgatt gaaaaaggaa gcattaatta aggaaaaata
    11041 tgaagaaatg ttttgctttg aaaagcaagg gtacgcacaa ggtcaccaat ggattgccgg
    11101 cattgatgaa gtaggaagag gaccattagc tggacctgtc gtggcagctg cagttatctt
    11161 gccgaaaaat gagatcatat tagggctgaa tgactccaaa caattatcag agaaaaagcg
    11221 agaacttctt tatcaacaag ttcaagaaaa agccctggca atcgggatcg ggatcgttga
    11281 tcagcagaca atcgatgatg tgaatattta tgaagcaagt aaattagcga tgatccaagc
    11341 agtagaaaaa ttgtccattg taccaagcta tttgttgatc gatgcgatga ccttggattt
    11401 gcctattcag caggaaaaaa taatcaaagg ggatgcacga tctgtgtcga ttgctgctgc
    11461 aagtatcatt gctaaagtat atcgagacca attgatgaaa gaatatgacg tgctttatcc
    11521 tggatatggt ttctcgcata atgcaggtta tggaacaaag gaacacttag aaggacttga
    11581 acgacaagga attacgccaa tccatcgtaa aagttttgca cctgtaaaaa aatatttaca
    11641 ataaaaaaca gccgctttta cgtatcttat tagaaagaga gacgaaaggc ggttttatga
    11701 tgtatcaaat agaagaaaat ttattgaaac ttgcttatac aaaaggaatc agtagtcaag
    11761 gaaaatgggc acttgctaac tggatgatgc agtatcctta taaagaaatc ggatttgaag
    11821 acatcattaa gataggtaaa ataaccaaac accgggagct gtttcgcaaa agttggcagg
    11881 aaattcacgc taactggggc aaaatccaat cgaaacagtc gtttatcact tgctttgatc
    11941 ccaaatatcc ccctcagctg cttcacttga cctatccgcc aattgtactg ttttataatg
    12001 gtgatctatc tttactttct tgtaacatgt taagcgttgt tggtggaaga cacacttctg
    12061 ctttagccaa aaaaacagtt tattatttac taaaaccagt gatagaagct ggatatgtga
    12121 tcgtcagtgg ctgcgcgaaa gggatagata catacggtca ccaagcagca atctctcatg
    12181 gtggacggac aatcgcagtc attggaacag ggatagagca agtctatcct gctgaaaatc
    12241 gtgacttgca agaggtgatt gcaaatgatc atcttctttt gagtgaatat cctcctcaat
    12301 ccggcccgaa aaagcattgt tttccaatga gaaatcggat tatcgcagct ttgtctaaag
    12361 gaacatgtgt ctttgaagcc aagaaaaaaa gcggctctct gataacagca cagcaagcat
    12421 tggatcttgg aagaacagtt ttttctgttc caggaaatat tttaacagaa cattctaccg
    12481 gatgtcatca tttgatccaa gacggtgcag tttgcacggt ttcgggtgaa gatatcttgg
    12541 cagaatttaa agaataacac catacttttc atttgctttt cttgacaaac gttttctgaa
    12601 tggatatgat tagctacgat ttgtatgaat cctcttttaa tagtaggaac acgacaatgg
    12661 aaaggagtcc attcagtaat ggcgtataaa tatttagtga ttgtagaatc acctgcaaaa
    12721 gccaagacaa ttgagaaata tcttggacga aactataaag taatggccag tgttggccat
    12781 ataagagatt taccgaaaag taaaatgggt atcgattttg aaaacaatta tgaaccccat
    12841 tatatttcta tacgcggaaa aggcgatgtc atcaaaagcc tgaaagccgc agcaaaaaaa
    12901 gctcaaaaag tttacttggc aagtgacccg gatagagaag gagaagcgat tgcttggcat
    12961 ttagcgtacc tacttgggtt ggatctgaaa gaaaaaaatc gggtggtctt caatgaaatc
    13021 acaaaagacg cagtcaaagc agcttttaag gaaccaagaa cgatcgatgt agatttagta
    13081 gatgcacagc aagctcgtcg taccttagac agaatcgttg gttattcgat cagtcctatt
    13141 ctctggcgta aggtcaagaa agggttaagt gcaggacgtg tccaatctgt cgctttaaaa
    13201 attattattg accgtgaaaa agagatccga gaatttgttc cagaagaata ttggagcatc
    13261 gacggtaatt ttaaaaaagc tcgcaagaaa ttcaaagcaa atttctgggg aatcgacggt
    13321 aagaaaaaga aattaccaga tgcacaaagt gtaaaagaag tcactgctag aattgatgga
    13381 acagacttcg aagttaaaaa agtagaaaag aaagagcgca aaagaaaccc tgcgaatcct
    13441 ttcactacaa gtagcctgca acaagaagct gcccgtaagt taaactttcg aacaagaaaa
    13501 accatgatgg tagcacagca attatatgaa ggaatcactt tgggtaaaca aggaacagtc
    13561 ggcttgatta catacatgcg tactgattct acccggattg ccgatacagc aaaagcagag
    13621 gccgcagcat ttattgaaga tacgtatgga aaagaatttt ctagacatga tcaccgaaag
    13681 atcaaaaatg ctcaaggcgc acaagatgcc catgaagctg tgcgtccaac gagtgtgctt
    13741 cgtacaccag atgaactcaa acaatattta gataaagatc aattgaaact ttatacattg
    13801 atctggtcac gttttgtggc tagtcagatg acaccagcta ttttagatac aatgaaagtg
    13861 acattagaac aaaatggtgt catcttcatt gcgaacggtt caaaaatcaa attcaaagga
    13921 ttcatgcaag tgtatgtgga aggtagagat gacggcaaag aagagaaaga aaatattctt
    13981 ccagaacttg aagaaggcga tgtagtccaa tctgttgata ttgaaccaaa acaacatttt
    14041 actcaaccac cagcacgttt tagtgaggct acgttgattc gtgcactaga agaaaacggg
    14101 gtagggcgac cttccactta tgcaccaaca cttgatacga tccaaagacg ctactatgta
    14161 aaattgacac aaaaaagatt tgagccgact gaactaggag aaattgtcaa ttcattaatc
    14221 tgtgaattct tccctcaaat cgtcgatatc catttcacag cagaaatgga aggagatttg
    14281 gataaaattg aagaaggcac ggaagcgtgg gtcaaagtgg ttgatcgttt ctataaaccg
    14341 tttgaaaaag aactgacgaa tgcggaagaa aaaatcgaaa aaattcaaat caaagatgaa
    14401 ccagcaggat tcgattgtga tgtctgtgga catccgatgg tgataaaatt agggaaatat
    14461 gggaagtttt atgcttgcag taattttcct gattgtcgga atacgaaacc aatcgtaaaa
    14521 gaaatcggtg tgacttgccc agtatgtcat gaaggacaag tcattgaacg aaaatctaag
    14581 aaaaacagac tattctatgg ttgttcccgt ttcccagaat gtgaattcac ttcttgggat
    14641 aaaccagtgg gcagaaattg tccaaaatgt gacggatatc tggtcgagaa gaaagtcaaa
    14701 ggcggtaaac aagtagtctg tgtaaatgga gattacgaag aagacatcca aaaataatag
    14761 aaaagaggtt ctttcattga aaatggtaaa tgttgttgga gcagggcttg ccggaagtga
    14821 agctgcttgg cagatcgcac aagcaggagt ccctgtccgt ttatatgaaa tgcgtccagt
    14881 caaatcgact gaggcgcatc acacaagtaa ttttgctgaa ctagtatgtt ccaattcatt
    14941 aagaggaaac agtttggcta atgcagtagg gttattgaaa gaagaaatgc gccgattgaa
    15001 ttcggtagta atcacttcgg cagatgaaac agcagtgcca gctggtggtg ccttagctgt
    15061 tgaccgcgat tctttttctg aaacaattac ccgaaaagtc aaagagcatc cattagtaac
    15121 agtgataaac gaagaaatta ctgaaatacc ggaaggaatc accgtagtag caacaggacc
    15181 tttgacttct catccattag cagaaagcat caaagcattc aatggttcag atggttttta
    15241 tttctatgat gcagctgcac caatcgtaga caaagctacg atcgatatgg ataaagtata
    15301 tttgaagtcc cgctatgaca aaggagaagc agcttacttg aactgtccga tgacagaaga
    15361 ggaattcaaa gctttccatg aggcactaat caatgccgaa gtggcagaac ttaaatcttt
    15421 tgagaaagaa aaatattttg aaggatgtat gccaattgaa gtaatggcac agcgaggatt
    15481 caaaacgatg cttttcggac ctatgaaacc agttggattg gaagatccta aaactggcaa
    15541 acgtccttat gctgttatcc agctgcgtca agacaatgct gcagcttctt tatacaacat
    15601 cgtgggcttt cagactcact tgaaatgggg cgagcaaaaa cgtgttttcc ggatgattcc
    15661 tgggttagaa aatgctgaat tcgttcgcta tggtgtgatg caccgaaaca gctttatgaa
    15721 ttcgccagaa ttgctgaaac cgacttacca atcaacgaaa cgcgaagatc tgttctttgc
    15781 aggtcagatg actggtgtag aaggctatgt tgaaagtgca gcgagcggac tacttgctgg
    15841 aatcaacgcc gcacgcttag caaaagaaat ggaaccagtc gagtttccac aagaaacagc
    15901 tattggaagt atggcttact acattactca tgcggaagga aaacatttcc aaccgatgaa
    15961 tgccaactat ggtctgtttc ctgaactgcc agagcgcatt cgagacaaaa aagcaagata
    16021 tgaagcaatt gcacaacgag ctttaaaagc taatgaggca ataaaaacag aattagaaaa
    16081 aactgcaatc ccaaatgagt aaggaaactc catgaagagg gtgagacgga tagtctcgct
    16141 ctttttcttg cgtttttctt aagaatttct caaacaaaat gtgaggaatt gcgccaattg
    16201 tgaacaaact tagaaatatt ggttaaattc tgacaattta tttgtctttt tataaacgct
    16261 gtgctaaatt attctctggg acaaaaggaa ggtgagaaaa tgacagaagc cacgtgggaa
    16321 caaaaatttt tgcgttattt gattgtcgaa agaggatatt cagaaaaaac gaaagaagca
    16381 tatgaagaag acattctcca tttcaaacat tttttggagg aatctggaga cgctgatctg
    16441 ctgaaggcgg aatattttac ggttcgtgtc tatttgagtg cattagctga tcatcgtcct
    16501 agctatagca gaaattctat cagtcgcaag atagccagcc tacgttcgtt ttatcagtat
    16561 cttctcaaag aagaagtgat aaaagaaaat ccattttctt acgttcatct caaaaagaaa
    16621 aatttgagac tgccacgttt tttttatgaa aatgaaatgc aagcactttt tgacagcgta
    16681 gcaggagata gtcctttgga tttgaggaac cgcgctttac tggaagtcct ttacgggtca
    16741 ggcatccgtt taagtgagtg tagcagtttg tcggtatcag acattgattt tgattcagag
    16801 gtcatgttga tccatggtaa agggaacaaa gagagatatg ctcctctagg ttcttttgct
    16861 caagatgctc tgcaagaata tttcagggaa ggccgccagg tattgatgac aaaatatcac
    16921 gaagagcatg atatcgtttt tgtgaatcat catggaaaac caatcacgcc tactggaatt
    16981 gaatatgtct tgaatcaaat gatcaaaaaa agcagtttgg atagtgatat ccatcctcac
    17041 atgctgcgcc atacatttgc tactcattta ttaaataatg gggcagattt gcggacagtc
    17101 caagaattgc taggacatgc caatttatcg actacccaga tctatgccca tgtaacaaaa
    17161 gaaagtttac aaaaaaacta tcgttctttc catccaagag cgtaaaaata aggaaaagaa
    17221 caaatttagg aggaaacaaa catggtcgaa tcaccatttc attcaaccac tatctgtgcc
    17281 gtcgaaaaag acgggaaatt tgctatggct ggagatggcc aagtaacaat gggtgaatct
    17341 gttgtcatga aaggcactgc aaagaaagta cgacgtatct acaatggaga agttgttgta
    17401 ggtttcgccg gaagtgttgc agatgcgttt actttagaag aaaaatttga aggcaaacta
    17461 aacgaatata acgggaatct acaacgcgca gctgtcgaat tagctcaaga atggcggaca
    17521 cagcaatcta tgcaaaaact tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt
    17581 cttgtttcag gtacaggaga agttatcaca ccagatgatg gtatcttagc tatcggatca
    17641 ggagggaatt ttgcgttggc tgcagcccga gcaatgaaaa actatgctca aaacgacatg
    17701 agtgcgaaag aaattgcaga aaatgcgtta aatattgccg cagatatctg tatctttact
    17761 aatcataata ttatcgtaga agaactgtaa gaggtgagta gatatgtatg aattaaaaca
    17821 aacaccaaga caaatcgtaa gtgaattaga tgaatatatc gtaggacagc acgcagcaaa
    17881 aaaatctgtt gccgttgctt tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat
    17941 gcaacaagat attacaccaa aaaatatgct gatgatcgga cctacagggg tcggtaaaac
    18001 agaaatcgca cgacgtttgg caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac
    18061 aaaatttact gaagtcggtt atgtcggaag agatgtagaa tcaatggttc gggatttagt
    18121 agaaaatgcg atccaaatcg tagaaaaaga gcagtacagc cgtgtataca caagtgctaa
    18181 aaaaaatgca gaaaaacgtt tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa
    18241 acaatccact aatcaatttg aacaaatgat gaatcttttt aatgctagtc agcaaaatac
    18301 cgctgaaccg caagaagaag tgacagaaga tattcgaatc aatcgccgta cagtcttgga
    18361 acaactacaa aagggtttac tggatagtcg tgaagtaacc attgaagttg aagaccaaaa
    18421 gaaacctgca ccaatgatga acaatggatt ggagcaaatg gggatcgacc taaatgaaac
    18481 tctaggtgct ttgacaccga agaaaaaagt ggaacggacg gtaacagtca aagaagcttt
    18541 agagttattg atcaaggaag aatcagcaaa attagtcaag gatgctgata tccatagtga
    18601 ggcaatcaaa ttagcggaag aaagtggaat tatttttatc gatgaaatcg ataagatcac
    18661 ttctaaatca cagcaatctg gagaagtatc cagagaaggc gtccaaagag acatattgcc
    18721 aattgtcgaa ggttcacaag taaacacaaa atacgggatc atccaaacag atcacgtcct
    18781 attcattgct tcaggagctt tccatttgtc taaaccaagt gatctaattc cagaacttca
    18841 aggacgcttc ccgatacggg tagaattaga tgatttgaca gctgaagatt ttgtgaagat
    18901 tctgaccgaa ccaaataatg ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa
    18961 tgtgtcagtt acgtttacaa aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt
    19021 caaccgtgac acagataata tcggcgcacg cagactgcat accattttgg aaaaactact
    19081 agaagatcta ttatttgaag caccagatat gcaaatggga gaaataacga tcacagaagc
    19141 atatgtggat gacaaacttg gctcaattgc ggcaaatgaa gatttaagtc gctatatttt
    19201 ataaaaaatc ggaggacgac aatgacagac ttgctaacga aaacccgcaa gataaacaca
    19261 cttttgcagc aaaagaatac ttttgacatg caagcagatt tgccttacga taaaatggct
    19321 gtcacactag gtggtattct tgacagcaac acctatatca taggaagtga cggagtatta
    19381 ctaggttata atgaacgtca cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag
    19441 aaacaatttc ctgaatctta tactgataca gtggcacagt tgatgaaaac tgaagctaat
    19501 atcccaatta ctagtgatat tactgcattt ccggtagagt caagagagaa atatccattt
    19561 ggtctgacga ctgtcgttcc gatctttgga gctggcgaac gtttgggtac gattatctta
    19621 tctcgtatgg aacaatcgtt tgcagatgac gatctagtct tagcagaata tggagctacg
    19681 gttgtcggta tgcagatcct ttatcagaag tctcgaagca tcgaagcaga tgtacggagt
    19741 gcaacggctg tccaaatggc aatcaacaca ttatcttata gcgaattgaa ggctgtacaa
    19801 gctatctttg aagcattgga tggagatgaa ggtcgtctga ctgcttctaa tattgccgat
    19861 agtatcggca tcactcgttc tgtgatcgtc aatgctttac gaaaattgga atcagctgga
    19921 atcattgaat ctcgttcgtt aggcatgaag ggaacttatt taaaagtatt gaattcccgt
    19981 tttaaagacg agttagcaaa acacagttat tagaagtaag gggactgggg cagatagaaa
    20041 tgtttgttcc agccttccca ttttaaaaag taagtcgaag ttggaggaat ggcaatgact
    20101 gttattattc aaaatgacca gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc
    20161 agtctgaaat caaaagaaca tggtttagag tatatctggc aaggtgatcc agcgtattgg
    20221 ggcagacatg caccggtttt attccctttt gttggacgtt taaaagatga tcagtatgtt
    20281 tatcaaggga aaacatatca aatggggcaa cacggatttg ctcgggatat ggattttgaa
    20341 gtcattgaac atggacaaga aatggctagt ttcatcttaa ggagtacgcc ggagacatta
    20401 gaaaagtatc catttgattt tgaattagtg atcagctatg aattaggagg agacggcatc
    20461 acggttcatt atcaagtggc aaataccggt gaaaaagaaa tgttcttttc tatcggtgga
    20521 catcctgctt tcaatgtacc tcttgaagaa ggattgtcat ttgaagattt ctatctggca
    20581 ttttctccat tgaaatcaag attacgccta ccattgaaag gcccattcat cgatatggat
    20641 caaaagacgt taggtcagac aaatacgaat ctggctttga atcgtgaact ttttgagcaa
    20701 gatgccttgg tttttgaaac aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct
    20761 tcccatagcg taactttaag ctataaagat atgccttatg tagggatctg gtctccttat
    20821 ccaaaagaag cgcctttcgt ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac
    20881 agtagtggaa acttgatgga aaaagtaggg atcaatcaac taggtgccca tgaaatcttc
    20941 aagacaaaat attccatcac agtgaagtaa gaggttgtga ccgagaagtt agcagggaac
    21001 accgtttacc aaggtgtgta aaaaagctat cctgtaagta ataagggagt gtgacatcg
//
LOCUS       DACENJ010000025        20997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.025, whole genome shotgun sequence.
ACCESSION   DACENJ010000025 DACENJ010000000
VERSION     DACENJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.025"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..386)
                     /locus_tag="IW748_000721"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="IW748_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            643..4032
                     /locus_tag="IW748_000722"
     CDS             643..4032
                     /locus_tag="IW748_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1103648.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            4035..5450
                     /locus_tag="IW748_000723"
     CDS             4035..5450
                     /locus_tag="IW748_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1103649.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETEMVQITLHKLLFPNGQL
                     PKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGASS
                     GMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVVLP
                     VHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVNIL
                     DYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKYAG
                     KKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPDKR
                     LAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLKEI
                     EAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLGVV
                     GMVFASFAIWLFIKKRTGVKK"
     gene            5447..7324
                     /locus_tag="IW748_000724"
     CDS             5447..7324
                     /locus_tag="IW748_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1103650.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            7416..8258
                     /locus_tag="IW748_000725"
     CDS             7416..8258
                     /locus_tag="IW748_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1103651.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(8410..9285)
                     /locus_tag="IW748_000726"
     CDS             complement(8410..9285)
                     /locus_tag="IW748_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1103652.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            9492..10523
                     /locus_tag="IW748_000727"
     CDS             9492..10523
                     /locus_tag="IW748_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1103653.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10612..10917
                     /locus_tag="IW748_000728"
     CDS             10612..10917
                     /locus_tag="IW748_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1103654.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            11096..13111
                     /locus_tag="IW748_000729"
     CDS             11096..13111
                     /locus_tag="IW748_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1103655.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            13113..13397
                     /locus_tag="IW748_000730"
     CDS             13113..13397
                     /locus_tag="IW748_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1103656.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            13423..14796
                     /locus_tag="IW748_000731"
     CDS             13423..14796
                     /locus_tag="IW748_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1103657.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14879..16894)
                     /locus_tag="IW748_000732"
     CDS             complement(14879..16894)
                     /locus_tag="IW748_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1103658.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16916..18130)
                     /locus_tag="IW748_000733"
     CDS             complement(16916..18130)
                     /locus_tag="IW748_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1103659.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            18351..19295
                     /locus_tag="IW748_000734"
     CDS             18351..19295
                     /locus_tag="IW748_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1103660.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19591..20535
                     /locus_tag="IW748_000735"
     CDS             19591..20535
                     /locus_tag="IW748_000735"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1103661.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7162 a         3846 c         3962 g         6027 t
ORIGIN      
        1 caacgatcac ttttaataaa tagctttcta aaatttgatt gcattgtttc aacttacgtg
       61 taaaagaaga ccggctgatg gaaatttttt cacaataata ctcaatcgtt ttacccggat
      121 tgacaataat ctcatttaat aaacgactgt ttgtagaact ttgcgacatt tccatcagaa
      181 catttaaata agctggtaca ttcaaatgtt caagacgata ccccaatttt tgtgaagatt
      241 gaatcgttac atccgttccc cagcggattt tgatctcttc gattatacct tacaaatgta
      301 gattctgaac taccaattcg tttggcaagt tcttttttag tcatccactt gtcagaaatg
      361 gacagaattc gaatgatttt ttccatcttt ctatagtttc gttccaacaa cctgatcata
      421 tgtttttctc cctcaaccct tttctttcaa tgaaacgctc attaatcgaa ggaatcaact
      481 tcataaatga aaccaattgg tatgaaaatt ggtgtgattc cctttagcgc ccttcgtata
      541 ataattatca tattaaaata aataaaatta tgtaacgcct gtttatttta ataattttca
      601 ctattcacgt caaaacagaa aaaaagaaaa aggaggggaa aaatgacgac cacagggaag
      661 aaactgaaag ttattttcat gctgataata ttgagtttat caaactttgt gccattatct
      721 gcaatagcag acactacaga tgatccaaca gttttagaaa caatttcagc tgaagtcatt
      781 tcggatcagt ctggaaaaaa agcactgaac atcaagctaa atgcgaataa caccagtgct
      841 gaaaagatag aaaaagaaat tggtctagtc gaaaattact taagtgatgt ggaaagaaaa
      901 gaaggagatg gctatgctta tcaggtaaat agcgggaaaa ttacgttgga aatctcatca
      961 aacactaaac aaactatcga tctgagtttt ccaatcgatc cagcacttta ccacagccag
     1021 gcaaacaagc tgatcgtcga taataaagaa tatgacatta ttgatgagac agaaaataag
     1081 aaagatacag atgtgtcagt accaaagcca gacgaaatag aagaagaatc atcaaaagaa
     1141 aacgaaaatt ctgtcagccc atttacattg cctacattat ccttgccagc tgtgagtgtg
     1201 ccatctaatc aaacgattcc tacagaatat acaacagatg atcagggcac ttatcctaaa
     1261 gccagttggc aacctacagg aaatacaaat gttcttgatc atcaaggcaa taaaaacgga
     1321 acaaatcaat gggatggtat aaattcttgg aatggagatc ctaatgatcg gacccattcg
     1381 tatatcgaat atggaggaac cggtaatcaa gcagactatg cgatacgaaa gtatgcaaag
     1441 gaaacaagta cacccggatt gtttgatgtt tatttgaatg ctcgtggaaa tgtacaaaaa
     1501 gatatcacgc ctcttgatct cgtattggtc gtagactggt caggaagtat gaacgacaat
     1561 aatcggatcg gtgaagtaaa gattggtgtc gatcgttttg tcgatacttt agcagatagc
     1621 ggtatcacag acaaaatcaa tatgggatat gtcggctact caagcgaagg atatagctac
     1681 agtaacggtg cagtacagat gggttcattt gattcagtga aaaatcaagt aaaatccatt
     1741 acaccttcac ggacaaatgg tggtactttt acacaaaaag cactaagaga tgcaggaagc
     1801 atgctatccg ttccaaatgg acataaaaaa gtgatcgttt tgctgacgga tggtgtacca
     1861 acattttcct ataaagtaca gcgggtacac gcacaatcaa gcagcaatta ttacggaact
     1921 cagttttcta atacgcagga tcggccggga aatacttctc taatctcaag aatctatgat
     1981 gcacctgacc aaaacaatct atccagaaga atcgacagta cgtttatcgc aaccatcgga
     2041 gaagcgatgg cactcaaaga acgaggaatc gaaatacatg gtcttggcat ccaacttcaa
     2101 agcgatccgg cagctggtct ctcaaaagca gaagtagagt ctcgtatgcg acaaatggtt
     2161 tcatcagatg aaaaaggcga tctttactat gaatcagctg atcatgcaac agatatctct
     2221 gaatacctag ccaaaaaagc tgtacagatc tcagcaactg taagcaatgg acaaataaat
     2281 gatccaatcg cagaaccatt catttatcag cctggtacac tttcagtcaa gagtgtgggg
     2341 acaagtccta caacggtcac tccatctatt tccatagaag gaaataccat caagagcaat
     2401 cagatctatt taggaaaaga ccaagaaatc caaatccatt accaagtgag aatccaaaca
     2461 gaaaatgagg acttccatcc aaatttctgg tatcaaatga acggcaggac aactttccag
     2521 ccaaacattg ataccaatga attagctgaa ttcggtatac catctgctaa agctcccgga
     2581 gtcagtcttc acatcaaaaa gttatgggaa gaatttgaca acaatctagc tgatcgtcca
     2641 gatcaagtta cttttgagat tcaacgggaa catacgacaa atgctgcagc ttggaaaaac
     2701 ggatatattc gaatcattaa accagctaaa gatacaacaa atacgtggga acgtgcagac
     2761 attgacaaat tatctgcaaa tagcggagaa agttatcaag agatattatc actacctcaa
     2821 tacaataatc aaggtcaagc attcagttac caaacaatca aagaattacc tgtaccagga
     2881 tacgattctc aacaaataga tgcaatgaca tggaaaaata ctaaacaatt cacaccgtta
     2941 aacttgaaaa taacgaaaaa ttcctctaca ggtgaaaagg atcttattgg cgctgttttc
     3001 aaattaacag gagattctat tgatacttta ctaacagatc atggcgacgg aacctattct
     3061 cttccagaaa atgtcaaatt gcaaaaagaa atgacctata cgctgacaga aacaaaagct
     3121 ccagaagggc atggattaag caaaaagact acttgggaaa tcaagatcgc ttctgatggt
     3181 acggtaacca ttgatggaaa aacagtcact acttccgatg atacgatcca gttgactatt
     3241 gaaaatcctt ttgttgaagt tcctgtagca gtacgtaagt atgcgatgca agggacggac
     3301 aaagagataa atcttaaagg agcagcattt tccctacaga aaaaagaagc aaatggtact
     3361 tatcagccaa ttgacagcca aacaacgaat gaaaaaggtc ttgccagttt tgattcactc
     3421 acacctggta aatatcgagt cgttgaaaca gctggtcctg ccggatatga tacttcgccg
     3481 ggaaattatg aattccaaat cgataaatat ggaaaaatca tttacacggg aaaaaatacc
     3541 gagatgacaa ataatgtatg gacgctcact catcaaaatc gactaaaagc gtttgatcta
     3601 acggtacaca aaaaagaaga caacggacag acattaaaag gagcaaaatt cagactgcag
     3661 ggaccagaaa tggacttaga atcgccaaaa gatggacaag aaacagatac ctttctattc
     3721 gaaaatttaa aacctggaac ttatacgctg accgaaactt ttacaccaga aggataccaa
     3781 ggtctaaaag agccagttac tatagttata cacgaagatg ggtcaattca agtggatgga
     3841 caagatcatg aatctgttct gtcaccagga gccaaaaaca accagatttc tttagacatc
     3901 acgaatcagg caaaagtacc attacctgaa acgggaggaa ttggccgttt aggaatctat
     3961 ctagtaggga tgattggttg tgcgttttct atttggtatc tttttttgaa aaaagaaaga
     4021 gggggcagct aaatatgaaa aaacttggtt ggcttagtat gtgtctcttc ttgttactat
     4081 ttaaaccagc ttttactcag gtagcaacag aaacagaaat ggttcagatt actttacaca
     4141 aattgctttt cccaaacggg caactgccga aaaatcatcc aaatgacgga caagaaaaag
     4201 ctttattaca aacgtatcga ggattaaatg gtgtcacatt ccaagtttat gatgtcacag
     4261 attcttttta ccatctacgg gaaaagggca aaacggtaga agaagcacaa gcagagatcg
     4321 caaaaaacgg tgcgtcttcc ggtatgttta ccgcagaagc aacaactaca actcttaaca
     4381 acgaagatgg tatcgcttct ttttctctgg ccgctaaaga tcaagaaaaa agagataaag
     4441 cgtatctttt cattgaatcc aaagtaccag aagtcgtcaa agaaaaggca gagaatatgg
     4501 tagttgttct tcctgtacat ggacaaaaca atcaaaaact ttcaactatc catttgtatc
     4561 ctaaaaatga agaaaacgac taccctgatc caccttttga gaaggtatta gaagagccta
     4621 gaaatgattt tacgattggt gaaaaaatca cttattcctt gcatacgaca attcctgtaa
     4681 atatccttga ctatcaaaag ttcgaattgt cagatagtgc ggatgaagca ttaacgtttt
     4741 tacctaatag tttaacgatt tcatcgaatg gagaaaagct gacagaaggc tttgtcatac
     4801 acaagaaacc tcacggattt gatgttttat tttcgatccc ttcgttggaa aaatatgctg
     4861 gaaaaaaact gaccatttct tatcagatgc agctaagcag tacagcacag gcgaacaagg
     4921 aaatcaacaa caacggaaca ctggattttg gttttggtgt cagtacaaag aaagtctctg
     4981 tatatacagg gagtaagcaa tttgtcaaaa tcgagacaaa taaaccagat aaacgattag
     5041 ctggcgcagt attccttatt aaaaacaaag caggaaatta cctccagcaa acagccaacg
     5101 gatacaagtg gacaaagaac gaatcagatg cgcttcacct gatttccgat aaaaatggcg
     5161 ctttttcaat ttccgggttg aaaacaggaa gttatcgatt aaaagagatc gaagcacctt
     5221 ctggttatat tttaagtgaa acagaaattc cgtttaccat ttcaactttt ctttctgagg
     5281 ataaagaggc ggacagtata ttgaaagtag tcaataaaaa agaaaatagc cgtccatttc
     5341 ttccaaaaac aaacgaaacg aaaaatacac ttttaggcgt tgttggtatg gtattcgcaa
     5401 gctttgcaat ctggttgttt atcaaaaaaa gaacaggagt gaaaaaatga aaaatcataa
     5461 aaaaataaac gttatgttag gagtcctttt ccttatttta ccattactca caaacagctt
     5521 cggcgcaaaa aaagtgtttg cagaggagac agcagctcaa gtcatccttc ataaaaagaa
     5581 aatgactgat ttacccgatc ctttaatcca aaacagcggg aaagaaatga gcgaattcga
     5641 tcaataccaa ggattagccg atatttcatt ttcagtttat aacgtcactc aagaatttta
     5701 tgcgcaacga gataaaggag cgtccgtgga tgcagcaaaa caagcagtcc agtctttgac
     5761 tcctggtaca ccagttgctt caggaacgac agatgctgat ggaaatgtca ctttatcttt
     5821 acctaaaaaa caaaatggga aagatgcagt ctacacgatc aaagaagaac caaaagacgg
     5881 agtgtcagct gccgcaaaca tggttttagc tttccctgta tatgagatga tcaaacaagc
     5941 agatggctct tataaatacg ggacagaaga actagatact atccatctct accctaaaaa
     6001 tacagtcggt aatgatggaa cgttgaaagt tacaaaaatc ggtactgccg aaaacgaagc
     6061 actaaatgga gcagaattta ttatttctaa agaagaagga acaccaagcg tcaaaaaata
     6121 catccaaagt gtcacagatg gattgtacac ttggacaact gatcaaacca aagccaaaca
     6181 tttcattact ggtcattctt atgacatcgg caacaatgac tttgccgagg catctattga
     6241 aaaaggccag ttgatcgtta atcatttaga agttggaaaa tataatttag aagaagtaaa
     6301 agctcctgat aatgcggaaa tgattgaaaa gcaaacaatc acgccttttg agatcctggc
     6361 aaatagccaa acaccagtag aaaagaccat caaaaatgat acgtctaaag ttgataaaac
     6421 aacacctcaa ttgaatggaa aagatgtcgc aatcggtgaa aaaattcaat atgagatttc
     6481 tgtcaatatc ccattaggta tcgctgataa agaaggaacg caaaacaagt acacaacatt
     6541 caaacttatc gatactcatg acgctgcttt aacatttgat aatgattctt caggaacgta
     6601 tgcttatgcc ttatatgatg gaaataaaga aatcgaccca gtaaattatt ctgtcactga
     6661 gcaaacagac ggattcacgg tttcagttga tccgaattat attccttcat taactcctgg
     6721 cggtacattg aaattcgttt actatatgca tttgaacgaa aaagcagatc caaccaaagg
     6781 attttctaac caagcaaatg tcgataacgg gcatacaaat gatcaaacac caccgtcagt
     6841 cgatgtcgtt actgggggca aacgatttgt taaagtagat ggtgacgtta catcagacca
     6901 aacacttgct ggagcagaat tcgtcgttcg tgatcaagat agtgacacag cgaaatattt
     6961 atcgatcgac ccatccacaa aagccgtcag ctgggtatcg gcgaaagaat cagcaacggt
     7021 ttttacaacc acaagtaacg gtttaatcga tgtgacaggt ctaaaatatg gcacgtacta
     7081 tctggaagaa acgaaagcgc cagaaaaata tgttccatta acaaaccgtg tagcatttac
     7141 tatcgatgaa caatcttatg taacagcagg acagttgatt tctcctgaaa aaataccaaa
     7201 taaacacaaa ggtacacttc cttcaacagg cggtaaggga atctatgtgt atatcggtgc
     7261 aggagtagtc cttctactga ttgctggact gtactttgct agacgcaagc acagtcagat
     7321 ttagtctttt tgcaagaatt ggaaaaagga gcagcggatt acgcaaatgc tcctttttct
     7381 tttattatca aacagtcatc acaaggagaa cacaaatgaa accaaagaaa aggaaacttc
     7441 tagatctgct gatgcttttc ttgcttctct caggaatagg gatactagct tatccatttg
     7501 ttagcgacgc gctgaataat tatctggatc agcagatcat ctctcattat cagcaacaag
     7561 cggtcaaaga aaatgaagaa gtcatggcta aaatccaaga aaacatgaca aagaaaaacc
     7621 agcaattagc aaaagaaggt ggaaatccag gtgcagatcc ctttactaag aaaaaagaac
     7681 ctgcgaaaaa ggatagaacc tattttgaga aacatacgat cggtattttg acgatccctg
     7741 agatcaatgt ccatttgcca atttttgatg aaacgaatac tgtgctgcta gaaaaaggtg
     7801 cctcattatt ggaaggaacg tcttatccta cggggggaga aaatacacat gccgttattt
     7861 ccagccatcg aggactgcct caagcaaagt tgttcacaga cttaccgaaa ctgactttag
     7921 gtgattcttt ctatatcgaa atcaatggac ggacactcgc ttatcaagtc gatcagatca
     7981 agacggtaga accgactgat acaaaagatc tgagaatcga aaaagggcag gatttcgtga
     8041 cactcctaac ttgtacacct tatatggtca acagtcatcg cttacttgtc agagggcacc
     8101 gggttcctta tcatgcaaaa gaagttcaaa aagaagtaca gaaagtatcc gagaacaaac
     8161 gatttttcct gtatggattg ttagctgcat gtatactgat tttcctcctt cttatatatc
     8221 tttttcgtca atcgttcaaa ataagaaaca gaagttagct attcctcatt tctataatat
     8281 atctattcga atcgttccgt aaacaaacag gctgaggttt gaaaaagaaa acttcagcct
     8341 gtttttatga tgatatcaaa gagagcctat caaacactat ctttcttatt actaaatgag
     8401 gatcttttct taagttttcc gatattcttt aagaaggcct ttttgcatca attcttgttt
     8461 gtttttcaag ttgcggatat tctcttcaaa attttgagaa tatatcactg agaataacgt
     8521 atttaatacg taatcgaagg caatttgaga ggagaaagtc gctactttag caaaatcata
     8581 ttcctcctgc gatataacaa gacacatatc agcctgtttg gccatttctg aatgctgatt
     8641 tccggtaatc agcaaggtag gggcctctac atgcttcaaa taggtcatta ttcgttcata
     8701 atgatggatg cgaccgctgt agctaataaa aatggcgcaa tcctcgctag tcaaattggc
     8761 tgcattccaa gaagaatcac tgtattcttc agccatgatc aaaaacttat tcaacttgac
     8821 cattttattc tggaatttcc ttgccgtgat ttgagaatca ccttttgcaa aaagaaagat
     8881 tcgctgtgcc ttgttcaata ctttaccgat ttgatctaaa tcttcatgtt caatctgcaa
     8941 ttgtgctttt tttattgact ggatcgataa atccgccatt ttctttgcaa tttgctgagt
     9001 gctatcttca cttgaaaaag gaaaattagg atcaatcgtt tgattgaact gagcaatcaa
     9061 ttgatgaact tcccgcccca gctcgaaacg gaattcttta aatccgctga atcccatttt
     9121 tttgcataaa cgaatgatag ctgaatggga agtatacgta gcttttgcta attcttcaat
     9181 cgtcatgtag actgcagctt cgatattcct acggatgtaa tcgacaatcc gtttctctgt
     9241 cgtggtaaac aactcttgtt ttgttagttt ttcttcaata agcatgatct gacctccaaa
     9301 ttctatctta ctgataaaca cttggttccg ctactatctt tttgattttt atccttactt
     9361 tgaacattaa tttcaccaaa ccgaatgata acaagtgttt ttctgaactt ttttaccaga
     9421 atagctatta taaatacttt ctgcgctttt ctaggatata ctgaaaacat caataaaagg
     9481 aggcaccact catgcttaca atcgcttatc ttggcaatgg aaagagtaca aatcgctatc
     9541 atttgccttt ttcaagaaaa cttacgaaca aaatcaatat caaaaaaatt ttttccccat
     9601 ctgaaccagt gtggaaaaca ttcgaagaca tcgaatatac tgatcagctg tctgatcttt
     9661 gggatgatcc tgagatccaa ttagttgtca tcactacacc tagtcaattt cattatgaat
     9721 atggaaaaat ggcactcgaa catggtaaga atgtactagt agagaaacct tttgcagaaa
     9781 cgtcaaagga agcagaagag ttatttgcat tagcaaaaga aaaaggtcta tttatccaat
     9841 gttaccaaaa cagacggttc gattctgact tcttgacagt ccaaaaagta atcgaaagcg
     9901 gaaaattagg ggatatttta gaagtagaaa tgcattatga ttatttcaga ccggaagtac
     9961 cagaaaatac gcatgaattt tctactgcga acagctattt atatggacat gcttgtcata
    10021 cagtagatca agttctttct tatttcggcg aaccagatga tatcagctat gatgtacgtc
    10081 aacttttagg gaaaggacga atgaatgatt acttcgatct ggattttttc tatggaaata
    10141 tgaaaatttc gataaaatcc agttatttcc gcatcaaaga acgtccaagt ttcgtcgttt
    10201 acggaaaaaa agggatgttc gtgaaacaaa caaaagatcg acaagaagaa catctgaaaa
    10261 tgttttacat gcctgatcat gaagactttg gtatcgattt gcccgaacat tatggtgtat
    10321 tgacctatat agatgaacaa gggagttatc atgaagaaaa agtaatttcc gaggttggtg
    10381 attacagccg tgtctacgaa gggatttacg agacattagt taatggcgca gaaaaagtca
    10441 tcaaagatga agaaacaatc ttacaaatga aaattttgga agaaggaatc aaaagcattc
    10501 aagaatcaga aggaaaaata tgacatattt gtaagaatcg ggtttgtttt ttgacgcata
    10561 aactgtctta ttgtacgatg aaaaagtata gaactactag gaggaaccaa catgacaaat
    10621 tatgacggtg aaaaacacaa acgattaaga ggttcaatgg cccctaaaac agaagaagaa
    10681 gtggctttag caggaactaa ttctggtgaa acagaagaca aagaagaatc attagctggc
    10741 acaaactctg gtgaaacaga aaataaagaa acagctttat ctggttcaaa ttctgtagaa
    10801 gaggaaacaa agcctaatga gcccaatcac tttgaaaaag atatcgaaaa agcggttgaa
    10861 gatgtcaaag aaaaatttga agaactgacc tcaaaaaata aacatgatca tcattgatcg
    10921 gacgaaatca attcataaaa gcaggagaca tttggtcacc tgcttttttt ctgtatccat
    10981 tcgatagaaa tcgttcggta cagtcgtaaa aaaaatccaa ctgtaagttg taccaaacac
    11041 ccctcgattt aaaagcgcta ccatactaga atataagtag gaggtgaaca gcgctgtgaa
    11101 ctacaaaatt agttatctcc tgaaaacacc aagagaaatc agtctagaaa aagcagcaga
    11161 gatcatcgaa gtaaatacaa gcgaggcgat caaacaatta actccttttg ccgaatatgt
    11221 ggatgatacc cgtatccgta taaagaaatt ccgagcaatc gactgggcag aaaaattttt
    11281 ggatattact cgatttgaag ctgtgtatac cgaacaagag cgaagagatc ttctttattt
    11341 gatggtttat tcgactgcaa cgtctgtttc tgtttttcat tttcaagatt ttcttcaggt
    11401 ttctaaaggg acagtcctgt cagatatcaa aaagataaga aatgaattag aagtggaaga
    11461 ttgtcagctg tcgtatacta ggaaaactgg ttttaggctg ctagggtcag aagcaatatt
    11521 aagaagagaa gcaaaaaatc gcactgctcg tttgatgcag tcacttgctg gaagatttgg
    11581 gctcacgtat ttaaccgcaa ccctttccat tgattattac gccaaagtta gagatttgct
    11641 agataaaacg atttcactat cagatcttcg attcgcacca aatcgaatgg atgaattagc
    11701 ttattatttg agtttgacaa ccattcgaat tggaaaaaca tccatggaac tggtaaaaga
    11761 tgctgaactg ttggcttctc ttgcagtcac tgacattagt cgacaagtat tgactggcct
    11821 tggctattcc gaacatcaaa attcggagat tatatttttc tctctttgct tagcatcgat
    11881 cgttgaagga gatgtccagg agcctgcact tgattttttg ctggaatgtt cttcagaagt
    11941 catccatcgc atggaattcc tgatggccgt agaatttgct tcgtttcgtg aattattgat
    12001 gaatgtcttt tatcacttgg tccctgccta ttttcgtatt tcctattctt tttatttacc
    12061 taatgtgatg atcgatcaaa ttaagcatca atatgcttcc atctacgaaa tgacaagaaa
    12121 agcattactt ccgttagaaa gacgaatagg gaagagcatc cctgaagaag agattggttt
    12181 tttcactata ctatttggcg gagagatcag aaaagcggac gcagaagaaa gaaatagaaa
    12241 aataagagcc gtgatcgtct gtccaagtgg cattagctct tcattgatct taaaaagtga
    12301 acttcagcaa ttattcccga tgatcttttt tacagagact aattcagctg accgcttaca
    12361 cgaggttgac gaaacttctt acgatattat tttttctact gtacctctca cacttactga
    12421 taagaaaaag tctctctatg ttctgcatcc aatcatgact gacttggaaa aaaatcagct
    12481 gatcaatcaa gtacaaagag attggctgat accaggattt tcaatgcctt ctgcccaaga
    12541 attgttgtcg gcgctactgc cttatatcga attaaaagaa ggcgtagacg aagaaaagct
    12601 ctatcaagta ttaaatcgta aaatgaacaa acttttagaa aagaaggagg atcaacgtcc
    12661 tatgctatct gaattattaa cagaagaaat gatccaatta tcggatcaac caaaaaactg
    12721 gcaagaagca atcacatttg ccgcacagcc gctattatca gaaaataaaa tagaagaaag
    12781 ttatatcaaa gctatgatcg agcgcgtgga gcagtatggt gcgtttatcc atattggcga
    12841 tcacatcgca ttacctcatg ctcgtccaga agatggcgta aacgaagtag ggatgtcttt
    12901 gctgaaactt caacaacctg tggatctgct tgataatcca gcccatccca ttagtttgtt
    12961 catctgttta gctgctatcg ataatgaggc acatcttcgg gctttggcaa atttaacaaa
    13021 gctactttct aataaacaaa atcttcaaga tttattacac gcatcaacaa aagaagaaat
    13081 attagcgatc ataagaaaag gagacgaatg atatgaaaat ttttgcagta tgtcaaagtg
    13141 gattaggaac aagttttatg gtagagatga acatcaaaca agcacttgca gatcttggag
    13201 cagatgtgga cgagtttgaa gtaagtcata cggatgttgg aagtgtatct agcgatatgg
    13261 ctgattattt cttcattgag cattctttga aagatacgct aacttctata cctgatgaaa
    13321 agctcgtacc tttgcaatcg atcattgaca gcgaagaagt aaaagaaaaa gtaacgaaga
    13381 tcttgtcaaa agaataaaaa tgattcaagg gaggaactat caatggataa attgttgaat
    13441 ttgtttgtgt caatcgtcag tcagccctcc atcctcgttt ctttgattgc actgcttggt
    13501 ctcgttcttc agaagaagaa attttcagaa gtgatccaag gaactatcaa aacatttgtc
    13561 ggattcctag tactgatcgg cggtgctggt ttaatcagta attcactaac cccttttgct
    13621 tcgatgttcc aaacagctct gcatactcaa ggagtagttc ctagcaatga agcagtagtg
    13681 gcgattgctt tgcaacaata cggtacacct actgctttga tcatgctagt agggatgatc
    13741 gtgaatattt tacttgctag attcacaaga tataaattca tttttcttac aggtcaagca
    13801 atgctttatg tttcttgtct aacagcagtc attttagtaa gcgcggggtt tactgcaaat
    13861 ttgtggatga tcttattagg cggggtcttc gaaggaacac tattaactat tacgcctgct
    13921 ctttgtcaac cgtttatgaa gaacatcact ggaaacaatc aagttgcgtt agggcatacc
    13981 ggaaatatcg gttatgcttc tagtggatgg atcggaaagt tttttggaaa caaagaccgt
    14041 tctacagagg acataaagat tcctaaaagt ttcagctttt tgcgagattc tactgtatcg
    14101 atcatgctct tgatgtcgat cgtatatgtc gcacttgctt tgttagctgg tcctggattt
    14161 gttgaaagag aactaagcaa tggagacaac gctgtaatct ttgcactgat ccaagctgga
    14221 acattcacag ctgggtttgt tatcgtcctt caaggggttc gaatggtgtt ggcagaaatc
    14281 gttccagcat tccaagggat tgctaaaaaa ctcgtacccg attctaaacc agcattagat
    14341 gttcctatcg tttttccata tgcacctaat gctgtattaa ttggcttttt tgttagcttc
    14401 tttgtaggaa cactatccat gtttgctatg ataggcttgc aaactacagt tatcattcct
    14461 ggggtagtag gacatttctt ctgcggagct gcttctggtg tcttcggcaa ctcgactggc
    14521 ggtagaagag gggcggtatt aggttctgct ttcaatagct tattgattag ttggctgcca
    14581 ttatttatcc ttccagtatt aggagatctt aaaatcgctt cttctacatt tgccgatact
    14641 gattatctag taccaggact tgtacttggg aaactaggag aatacggtca aatagccttg
    14701 acagttggaa tcatcgcttt ctttttactt gtcgtattat ctagctttct ttttgccgga
    14761 aaaagaacaa acattgtttc taaagaacag aattaagcaa acttcaaaat aaaaattgct
    14821 tggacaatat cagaagaaga tttaaagtct tctgatatgt cgcaagcagt tttttgtttt
    14881 atgcatttat ttttgaatag cttgttaaga caagtggctg ttttgctaga agaggataac
    14941 ttttaccatt cattttcact gttccgcctg ttctatcaga agtaatcacg atcttatctt
    15001 ttgttttttc aagcgaaatc tttccatctt tgataggtag cgtacaattc agtgtttcaa
    15061 atgaatctag attaggttgt atcataaatt cttcataacc tggctttgtt ggatacaaac
    15121 cgataaaata gcgtcctaaa agatagattg ggcttgctcc ccatgcatgg cataagcttt
    15181 ttccataagg atctccatac atactgtact tttcatttcc tgtttgagta gggtcgtatt
    15241 cttcccagaa agttaccgca tcatgagata acatccctcc ccaataatca agaatcgttt
    15301 ggtagacgat attcgtctgt ccgagtttgc ataaagcatc ttgttcatag aatttgaaat
    15361 atggagtagt aatctgtgtg atagtgtcat tcaataagac attctttaag atcgattgct
    15421 gttggtcttt gttagcaaaa tcgaataaaa cagcgaaaat attcgcatga cgcgtcacat
    15481 ttcttctccc agattcatag caatcgataa aagcgccaag ttcttcatcc caaaagtagt
    15541 caaataggtt ttgtttcaat agttgatatt tttcttgata gagatgagta tgataatttt
    15601 cgttctctcc atatgtttca gctaagatat ctccgcaagt gatcatggaa tgataggctt
    15661 tcagtaataa gacttgttct gcagcaagcg ttccttcttt atccatttca gaccaatcaa
    15721 taaatatcca atcttttgct ctcccataaa taaaccctaa ttcattcgtt tgctccatca
    15781 agtatctcat taatgattgc atcttagggt agacgatgcg taaaaattca tagtctttcg
    15841 tcatcatata gtggttttcg ataccaatca accacaagat cgaatagtca acgatagtat
    15901 tcatatgctg cttgatttca ttttgtcctc ttaatgctaa tatcgttcgt ttattgattt
    15961 cctcgtcaaa aaaaagatat tgattgatca tgaatgattg atacgcatct ccagaccaaa
    16021 tccagcgatc tcgtttgata ccgtcgataa aaaacaaatc actacataac ttgaaggctt
    16081 cttctgatac tgaccagatc tgattcagta aagaagaatc gcttgaaaaa gcagcttgtt
    16141 tcgaaagaga taaaaattca tgaaaaactt gaagttgaac cattccagta gaaacctctg
    16201 gtataaacaa atagcgaaaa gcgcgtttac gagctttcat ttttcttgtc attcctattt
    16261 gcttatagta gcaataatcg atatccatag cttctacatc ggactctcca taacatagag
    16321 tgatagattc gatatggtca tccagagata ttagttctac acagccattc actgctcgcc
    16381 caaaatcgaa taagattcct ccgccaatct ccgtttcagt gactggatgg acgcattctt
    16441 tttgataata gatttgattt ggatcttttg cgatatcgac ataaagcgga ctccagccag
    16501 ctggatattc ttcaataaaa ttacttgctg tccagccaat atctgatttt acttcgtctc
    16561 cctcgataaa catcgctggt agccctgatg tatgtcctac aaagatacga atttttgctt
    16621 taccagctgg acaagtcagc cattttccac atggatgttt ttgtccatta atttctacat
    16681 acccaactcc ttggatcgtc actttaaaca tggaaggttg atcaagaaag taatatctct
    16741 taaacttgac gttccgatgg caatcatcca tgtaccagta cgctggccag tcaaaacctc
    16801 gttcttcccg ttggaaattt tgtagcagtc catggcggat ctcaaagtct ccaggatacc
    16861 aaagccatct atctttatgt ttttcatttg tcattttaca cgacctccat caaatttatt
    16921 ttgttgtcaa ttgttctttt atttcttcat ctactcttat cactgtcttt ctattcgtcc
    16981 tgtgtactac tagaaggcta ataagcgcgc taatcaacag caagatactg ccattgacga
    17041 aaacagcaag tgttttactc ccaagagaag caaaagagag aaagagcaaa ccatatggtg
    17101 caatgaaagt acctaaatta gcgccaacca ataagattga agtattgaaa ctctccgcgt
    17161 acttgggcgc ttggatactt acttggttga ataagtaagg aatgaataac gaaaaagaaa
    17221 ttcctgttag gatcgcacca aacccaatca catacaggtt tatcacagtg gcaatcaata
    17281 caaagccgat tcccatcagt agtaaagaaa gtgtaagcat atagcgatgt gcttttgtct
    17341 gaagccagcc aaaacaaact ccggcagcca tacttgccaa ttgcatgatt gttaagatat
    17401 tcgctgcatt agtttcgttt cctagagaat aagtcgtcag gactgtggct aatttaatcg
    17461 atacagcatt atataccacg atgaccagca agagaaaaat accataacta atcattttag
    17521 gattcgtttt gattacttgt cttgttgggg ctgtgccatg cgattgaagt tcagttgttt
    17581 caggtgtagg aacaaacaaa acaaacaaga caagaatagg taagctgatg gcgtagacta
    17641 aaaaggtata ttgccaaccg aactgtacta gctgtcctgc aacaaatgtc attaaagctg
    17701 caccaaatcc ttgaaatgca ctttgaaaac caatcataga ggctcgttca tctccgtgat
    17761 aaaaggcagc aatcatgctg actgccaaag aattgatcaa tccaaagcca atacctagtc
    17821 cagctcttga agctaagaca atactgtaac tttgtgagaa gagggggagg ataccacaaa
    17881 tcgatgcaat cactaatccg atgatcacag tttgtttttg tccgattttc ctagcaataa
    17941 aactactaat caaaacaaaa atagtgacca taaaagaggg agcagtcgtt aataattcaa
    18001 cagcagataa agaacgcgtg gcataagtag ctgacatact gggaattgca gacgtgactg
    18061 caccagcaga agtcaaaata agagagactg acagcagaga aaatttcatc atctttttat
    18121 tcataagcat gttcctttca tttttgtaag cgcttatcaa aaatatagta tgaagtggtt
    18181 attctctcaa tagcggaatc acaggaagtt catgtcccaa ccatagaaag tctttatgaa
    18241 accaaaaata gctttgcata taatagcagt acagcgtgaa ctaataagcg aaagaaaccg
    18301 cttacaaaaa ggagggagtc aattggaact agctgaatta gacatttttt ttgcgtaacg
    18361 aagaatcaat cgaaactctg cagcgtgaac aaggaatgaa tatcaatgat ttaaacttag
    18421 cttaccatct tcagacaccg ccaaaaatcc accgagggga tctatttcaa cagggaaata
    18481 ttcatatcag taaacaccgt cgatatgcgg aagttcccga gcatacacat gattttgttg
    18541 aaatcaatta tatgtatagc ggtacatgtt tacaaattat taatggagaa aggatagaga
    18601 tggaagaaag tgacgtggtg atcattgata aagaagctac tcaaagaatc gaatacacag
    18661 ggcaagagga tattttaatc aacatcttat taaaagaaga aaccttatct acagagatat
    18721 tgaatcattt ggcaagcaca aataatctac tttcttcttt tttgataaat gcatcaaaag
    18781 aaaaagggga acatacacaa ttcattcatt ttcgcggcgg aaaaacaagt cgtttgacgc
    18841 atttgattga aaatatgctg ttgatttatt ttgatacata cagtcataag atccgttcgt
    18901 taaatctcta tacatcacta ttactgatcg aattgactca tttattagaa caacaaagct
    18961 tttcatcagc agatggatgc gcagaaaatg agatgcttag agtattagat tatatcgata
    19021 agcattttac ttcttgtacg ttaaaagaaa cagcagctca ttttggctac aatccggtct
    19081 atcttagtaa taaattgaaa atgtattttg atgcttcctt tcaagaactg gttctaaaga
    19141 aaaaattcga ctttgcgttg gaactgattc aagaaacgag tttttcgttg gaagaaatcg
    19201 ctaatcgtat cggatataaa caaacgactt ctttattcaa attattcaag aagtattcaa
    19261 catgtactcc caatgaatgg agaaataaac attaagaaga aaatctttca ctaaagcata
    19321 tgaaggaaaa ttgttttagg tgcgattgct gtcttattat atcgttgcag cgaatttttt
    19381 tggatttatt ttcagttcta aacttttggt tgtattatca ggggatatag gtgtaaacat
    19441 ttgtcattta tctgttatgc gtattcagga attcgtttcc atcaaaaatt ataataacaa
    19501 ctattataat taacatgttg ataaaatgaa taaacaagac tatacttttc atgaaagtaa
    19561 caaatagtta tgaaaaggag ctgtttattc atgaaaaaaa caagtcgtaa agtagtaatc
    19621 gtcggtacag gttttgttgg aacaagtatc gcttatgcaa tgatcaacca aggaatctct
    19681 aatgaattag tattgatcga tgtgaatcaa gaaaaagcag aaggggaagc attggacctt
    19741 ttagatggta tggcttgggg cgatgaaaat gtggctgtat ggtcaggtgg ctatgaagaa
    19801 tgtaaagatg ctgatatcgt tgttatcaca gctggtatca accaaaaacc tggtcaatct
    19861 cgtttagatt tagttaaaac aaatgcgtca atcatgcgcc aaatcgtaaa agaaatcatg
    19921 ggatcaggat tcgacggtat tatcgttgtt gcttcaaacc cagtagatat tttgacatat
    19981 atcgcttgga acgaatctgg tctgccaaca tcacgtgtta tcggtactgg aacaacattg
    20041 gatacaactc gtttccgtaa agaaatcgca ttgaaattaa aagttgaccc acgcagcgtc
    20101 cacggctata tcttaggtga acatggtgat tcagaagttg ctgcatggtc acatacaact
    20161 gtcggtggta aaccagtgtt tgaaattgta gaaaaagacc atcgtattgc ccaagatgaa
    20221 ttagatgtga ttgctgataa agtccgtaat gctgcttatg agatcattga tcgtaaaaaa
    20281 gcaacttact atggtattgg tatgagtact gcacgtatcg taaaagctat cttgaataac
    20341 gaacaagctg tattgcctgt atctgcttat ttaactggtg aatacgacga aaaagatatc
    20401 tttacaggtg tgccatcaat cgttgacgaa aatggtgttc gtgaagttgt tgaattatca
    20461 atcaacgaag aagaaaaagc aatgttcagc aaatcaacaa gtgctttaag agaagtattg
    20521 aacactgtat tataagaaac aatgaatatg aactaaaaat cctaaaactg cttaacagct
    20581 gaaatagctg ttaagcagtt ttttattgat atctaatagg taagcaaata aacagaatgg
    20641 ttaaaattaa tcggtccgaa aatgtatccg tacatatgaa catatgaaat ttaatctgtt
    20701 gattacaata agcgaatcgt tatcaatgtt tatctgtggt taattaaaaa atccttccgc
    20761 aacggcaaat acaatgattc atatctaatt cgttcatatt tgattaactg aataaagaaa
    20821 ctggttttta tcattcaatt aaacgacaaa aaaagtaaga aaaactgcaa aattttttta
    20881 taattttgca taagcagatt ttgatggaaa aatgacgatt tttgataaaa aaccgttcga
    20941 gagaggttta tcatttttta aatctacttt gtataatata tattatgtaa actaaaa
//
LOCUS       DACENJ010000026        20643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.026, whole genome shotgun sequence.
ACCESSION   DACENJ010000026 DACENJ010000000
VERSION     DACENJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.026"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            66..767
                     /locus_tag="IW748_000736"
     CDS             66..767
                     /locus_tag="IW748_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1103662.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IW748_000737"
     CDS             764..1885
                     /locus_tag="IW748_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1103663.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IW748_000738"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IW748_000738"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1103664.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IW748_000739"
     CDS             3264..4172
                     /locus_tag="IW748_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1103665.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IW748_000740"
     CDS             4207..4479
                     /locus_tag="IW748_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1103666.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IW748_000741"
     CDS             4490..5536
                     /locus_tag="IW748_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1103667.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IW748_000742"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IW748_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1103668.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IW748_000743"
     CDS             complement(8547..9218)
                     /locus_tag="IW748_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103669.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IW748_000744"
     CDS             complement(9211..9705)
                     /locus_tag="IW748_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1103670.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IW748_000745"
     CDS             complement(9725..10945)
                     /locus_tag="IW748_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1103671.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IW748_000746"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IW748_000746"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1103672.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13124..14035
                     /locus_tag="IW748_000747"
     CDS             13124..14035
                     /locus_tag="IW748_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1103673.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEISKESNRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFNKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            15033..15284
                     /locus_tag="IW748_000748"
     CDS             15033..15284
                     /locus_tag="IW748_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103674.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFVESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            15367..15639
                     /locus_tag="IW748_000749"
     CDS             15367..15639
                     /locus_tag="IW748_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103675.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            15729..15968
                     /locus_tag="IW748_000750"
     CDS             15729..15968
                     /locus_tag="IW748_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103676.1"
                     /translation="MIDISIPEDLKPDPATIGNSGMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMIFGDGEVANKAGIPIGKWTVDFHEAGSFFVL"
     gene            16115..20410
                     /locus_tag="IW748_000751"
     CDS             16115..20410
                     /locus_tag="IW748_000751"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ1103677.1"
                     /translation="MYAKTKMNWLKALALVALSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKLNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KNMNGKVQAEIKDLDPGKYVLKEIKAPKGYALSDETIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKIANKDLVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKERYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVT
                     FTLYNGKGQAIGKYTTDQNGEILVKGLVANNYYFTENVPLESFQPNTGKQAFTITFRQ
                     NAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQEGKEITSILTFIADKANGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAVHVDINDDNQSVRVNNPTVHTTAVFEDGLTIADPTGLFTLNDTVEINE
                     ILPGKAYLVKGILMDKATGEALLVHGQPVESAVGFIAKAENETVIVPFTFDLTGLNGR
                     EMVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLIVGKTYTVNGVLMDKVTEKPVLVDGKEVSSTLTFVAETTDGTVNLDFILD
                     AHALRSKEVVVFEDLFREGVLLASHADITDKDQTIKIVSPTITTKATNANGGKEILAL
                     PNQKVVEWVQVNGLAIGQIYKLIVQGYLTPDGTPFEGTHAEKVFMADKEMMEFLFEFI
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQFVEAPKPPQPVK
                     PNEPQPVKEVKQAAMVPMLPKTGSEDNVLWIMIGMIFVLAAGLIGYDLYKVRD"
BASE COUNT         7026 a         3538 c         4330 g         5749 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaaccatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggatgagc aagatagtgt gaaataactt ataatcaatc gaaaaaccta ttaaatcaac
    12961 ctttttgagt agatgcaaca ctgaggatta tctgtataaa agattttaaa ctgtcatttg
    13021 gttatcacgg cgatactaaa attaacaaaa ttggaatatt ttttcttgaa gatttttagc
    13081 tgtcagtata tgatgaaagc atctcatagg aggtgctttt ttcatggttg ataattcaag
    13141 tggaagggtt cttaaatctc taagaaaaga gaaaaaacta tctcaaaaaa agttagctga
    13201 tttagcaggt atttcacaaa gtactttagt taaatatgaa aaagggtcaa gaaaaatccc
    13261 taaagacgta gataatactc taagcaagat tttgaatata gaaactctaa taaaagatga
    13321 ggaagataag attgagattc tcatcggtca actaattgca tacagagata tgaataagtt
    13381 acttaacaaa gagttagctg ataatatagg aattagtgaa gtattactca gttatgtgtt
    13441 aaatagaaag agaaatccgt caaaagaaat gcaaaaaaaa atcgatattt ttctattaag
    13501 taatgaaaaa gaaatttcga aggagagtaa tcgtgatagt gagatattta gtttatcaaa
    13561 agacgataaa attgttatgg gaaaaagaat acgagaagtc cgaaagaacc gggaagagac
    13621 gcttgaaaaa tttggtaaaa actttaccat ctatacaggc aaaaatgtta taagtagatg
    13681 ggaaaaaggt attaatattc cagatattga aaaactaatg aatattgcct atcttggtaa
    13741 ggtcactgtt ccttacctta tgtacggaga agattataag aatatacttc caaaagatga
    13801 aagagttagc gattttaaca agataaattc ttttagtatg ggattaagga tgaggaaaat
    13861 taggaaagat tattatctag aaagggagga gtttggaaag cttttttctc cttcaatttc
    13921 aaaatggtcc atagataggt atgagaatgg tagagatata cccaatacta atcgaattat
    13981 ccaatatgct tatataggaa atctttcttt agaatttttg atttacggga tataaaaata
    14041 gtttacgtcg atacttatta agttatcgac gtttttttat tatagaaaaa tttataaaag
    14101 gaggtttctt gatatgataa gaaacaaaaa gggtttgttg aaagttgctt tactggcagc
    14161 ttttttctaa gtaacttttt gacaagctca atggcaacac aagcggaaga agtaatacaa
    14221 gaaataactg ttatgaatac cagctagata aaatgggcta ggtatgttat ccaacaattt
    14281 agtaatcact agaagcctag cgaaagttcg cttgataagt tatagatgag tattgatttg
    14341 tagtctttcc aaatcaaaag ggtacagagc cacatgaaat atgttgtcct ctgcagcgtc
    14401 aggatgggaa tccataccat ggaaattcgg agatctgtga gggaatactt acgtggttaa
    14461 tatgtcggtt gtgttggacg tagtaatggt catagtgggt agattgactg aattcttata
    14521 tgagaaaatg aaaccaacaa gaagataggg ttatttatac agaattgggg atcttttcta
    14581 gaaaagtaat cggacaatgc gagattcgta cggtcttttt taaatttctc ttaaaaaaac
    14641 ggcatcgaat gggttgggtg attgataagc aatgaatcat agactgatat agaataaaaa
    14701 aatgattcta tttatctttt tacatctatc gttttttcaa gtaaccgcaa attgtatcag
    14761 cattcaacaa tttttgtata caagttctag taaaaggatc tgaagaaaaa gttggttgtt
    14821 gtgaagataa aaaaagaatc aggttttact gagtttttct tcaaaatttg tgttattagg
    14881 ttaggacaaa gataagaaga agtatgaaga tttaaaaaag gaatgaggaa aagatagtaa
    14941 tttccgatta ttggagtttt tttattttat aaagaagaag cgaacaagtt gtgattccta
    15001 gatttcgcgc gtgattaatt aatgagcaaa aaatgcggaa agttttagtg tttaatacac
    15061 gaggacaaag tttcttaggt attgaaggca cacgtgtaac ggttgatggg tggtgtgatt
    15121 gtttcgaggt tggaaaacat gttgaactta tggcatttac tgagctaaaa gataagaaca
    15181 acgtggaatt tgtcgagagt gatattggtt gggacgatca tgaagaagtt tatggaaaat
    15241 ttattttgcg gaagggaaat tcttatacga atggggaagc ataagtagcg atttgttaga
    15301 gatcattgat aggattgaaa ttgtcgggaa tatttatgag aatctagagt tatttgaggt
    15361 gatagaatga gtgatggttt tgaacaagta tcaataatta aaaatcaagt tcgggagatt
    15421 cttagaaaaa aaggctattt ggtagatagt tattttgaag gagattacga aacatgggta
    15481 ggtgtctatg ctcgtccaga gaataaacct acctatttag atcctacgac gtcagaagat
    15541 gcctatttac aaaataggta tcgagtagat ggttttaagc aagactttgc ggagtggttt
    15601 gaatgggaaa ttgaaaacgg agaagtaaaa gaagaataag cgtacttgtt gtatcaaaat
    15661 aagtcagtag aacaaccaaa ccagtagcta gtaatgacaa taatactggg gaatcggatc
    15721 attatggtgt gattgatatt tctattccag aagatcttaa gccagatcct gcaacaattg
    15781 gcaacagtgg catgattttt gattcgttag atgaagctat gtcatatggt tggaaagaaa
    15841 ttaaggatga aggtagtaat tggtatggca tgatatttgg tgatggtgaa gttgccaata
    15901 aagctggtat tccaattggg aaatggacag ttgatttcca cgaagcaggt tctttttttg
    15961 ttctgtaatt atagtgggag ggtaaaatca ttgatgaaat gtgttcagat aagagcgcca
    16021 gataaaaata gaagagacag ttcacgtcgt tatcactttt ggtaacggtg ttttgtttta
    16081 ttatataaaa ttctcaggaa aaggaggaat tcttgtgtat gcaaaaacga aaatgaattg
    16141 gttgaaagca ctcgcattgg tggccttatc tcaattctgt ttcttggtgg gaactgctca
    16201 agcacatgca gaagaaacga cggctacaca acctacggtg gaagccattg cccaagtcgc
    16261 ttctgaagtg acagaagtcc aaccagcaga agccggggtc ccagctggtg aagccactgt
    16321 tgaacaggcc cctgtgacag aagcgccaac ggttaattcc aaaacgactc tcgatcagac
    16381 gattggggaa acacaacacc cagggaatag tcctgttact tcacaaccag ttgaagaaac
    16441 tgcctcaatt acaacccctg cttcagaaac gtccactcca gaaggagaaa aagctgccga
    16501 agcaacgcct tctgtgaagc cagagtcttc tgagaaacaa gcggaagaaa agcaacgaat
    16561 agaagaaaca gtggaacaac cagcggctac tgctgacaaa gaaggaaaaa atgaggaaaa
    16621 cgttgaaaaa acaatctctt caaccgcagc tagtcaaaat caaccaacga aactaaacaa
    16681 agaatctacg gctagtattg aagatcaaaa gaaaaaagag caggacattc caaaaacagg
    16741 tacacctaca ctgttagtaa cagtaccaac caatcgttcg gttcgtgttc gccgtgcaac
    16801 caatacagct gttttaggtg gtcggaaaat tgattggggc gaaaatattg gtgttacttc
    16861 tagtccgaac aatccaaact tcactttaaa ttatatggtt cgttttgaga ttaatggaca
    16921 agtggcgttt tgtattgagc ctggtgtctc agccgcagaa ggtcaagttt atacagctac
    16981 tgctttggaa aactatttga aaaattcaaa tgtacggaaa aaaacaagct tgatctctta
    17041 tttaggatat gtcacaaata aagacaaatc cgatgaacaa tacattgcgg cacaatttat
    17101 gatttgggaa gctttgggca caaaaatccc aatcaaaaat atccgtgttg attatgatcg
    17161 tcgaaaagca gaaattcaac gtctgcttaa taattttgat gtaagagcta cttttcataa
    17221 tcaaacacat acgataaaaa ttggcgaaac gttaaaagta aaaagtggca atgactttct
    17281 acaacgtgta aaaggcattc aaaagccaaa agggatcaat gtatctattc atgggaatga
    17341 agcatggatc acagtcacaa aagaagcacc agaaaaggca aaaattcatt tcaatcagat
    17401 tgaagtattg ggtacgccat tagcgtatcg tcgtccaggt gcccaaacaa ttggtatatt
    17461 gaatcctttt gaacctggtg actctgttgt caatttgact attttgaaac agggaaacat
    17521 tcgtgtcttt aaagaagatg cagaaaccgg tgtgaaagca caaggcgcag cttctttaga
    17581 aaatgcggtg tatggcttgt atcaagcaga tgggaaaaag atcaaagaaa ttaccttgaa
    17641 aaatatgaac ggcaaagtac aggcagaaat taaagatctt gatcctggca aatatgtttt
    17701 aaaagaaatc aaagcgccga aaggctatgc actttctgat gaaacaattg aaattgttgt
    17761 tgaaccagga aaaactgtca atgcgaccgc aaaagatcaa gtgatcaaag gaaccattga
    17821 tttagtgaaa attgctaata aagatttagt cgattcaacc aatccagata acaaaccaaa
    17881 attagctggc attgaaatct ctttgacgtc aaaaacaact ggcaaagtgg tgaaaaccgt
    17941 tgtgacagac aatgatggtt acgctacttt tggtaaaaat actgttgtct ttgataccta
    18001 cattctttct gaaaccaaag gaaaagaagg gtataaacta tttgaaccac ttgaagtcac
    18061 aatttctgaa aatgggcaaa ctttccatta tgttttagaa gataaagtaa ttgaacaacg
    18121 tcttaaagtg gtgaaagtgg atcaagaaac aggaaaagtg attccacttg ccaatacaga
    18181 attcaagatt tttgatactt tagccaatcg ttatgtcaca atggagattc caaatgatac
    18241 agaaacaacg gatatcttta aaaccaatga aaaaggcttt ttcctaacta acggtacact
    18301 tacttatgga aaagagcgct atcgtttgga agaaatcaaa gcacccgtaa attacgtttt
    18361 gaatcaaaac ccattagtgt tctcgattac caatgataca gaaccgatca aagtgatcca
    18421 ttttgcgaac cgtcaagcac ggaaaagtgt aaaattgttc aagtatgaag aatggaacaa
    18481 gaagaaaaca ccacttgccg gtgtgacgtt tacgctatat aacggaaaag gtcaagcaat
    18541 tggtaaatat accaccgatc aaaatggtga gattttagta aaaggtctgg ttgccaataa
    18601 ctattacttt accgaaaatg taccattaga aagtttccaa ccaaatacag gcaaacaagc
    18661 attcactatt acattcagac aaaatgcgcc agatgatggg gaaacgttat tggtttctgt
    18721 acgtaactat ttgattccac caaaattaga aacacaagcg tcaaataaag ccgatggtga
    18781 aaaagtggtt gatccattag aaactatcgt cattaaagat agtgtttctt ataccaacct
    18841 gtttgtcggc aaagaataca ctatttcagg tgtattgatg aacaaggcaa cgaatgcgcc
    18901 aattttacaa gaaggcaaag aaatcacaag tatcttgacc tttattgcag acaaagccaa
    18961 tggcacaaaa gatcttgaat ttgtggttaa tgctagtgtt ctgaaaggtc aaacaatcgt
    19021 tgtctttgaa aaacttttcc gtgatggcat tgaagttgcg gtacatgtgg atatcaatga
    19081 tgacaatcaa agtgttcggg taaacaatcc aacggttcat accacagctg tatttgaaga
    19141 cgggttgacg atcgcagacc caacggggct gttcacctta aatgataccg ttgaaattaa
    19201 tgagatcctt ccaggaaaag cttacttggt caaaggaatc ttaatggata aagcaactgg
    19261 tgaagcctta cttgttcatg gtcaaccggt agaaagtgct gttggcttta ttgccaaagc
    19321 agaaaatgaa acggtgatcg taccatttac ctttgatttg acaggtttaa atggtcgtga
    19381 aatggtcgtg tttgaaagtc tttttagaaa tgacgaagaa ctagcaaacc ataaagacat
    19441 caacgacaaa ggtcaaacgg tacgagtaac gaacccaagt gtacgtacaa cggctacaca
    19501 taaacccact ggcttgaaag aggttaatcc attaacgaaa gtcaccatta cagacgctgt
    19561 gaaataccac gatttgatcg ttggaaaaac atacacagtc aacggtgttc taatggacaa
    19621 agtaacggaa aaaccagtgc tagtagatgg aaaagaagtc tcaagtacct taacgtttgt
    19681 ggcagaaaca actgatggta cagtcaatct agactttatc ctagacgcac atgcgttgcg
    19741 aagcaaagaa gtcgtagttt ttgaagatct gtttagagaa ggcgtattat tggcttctca
    19801 cgcagatatt accgacaaag accaaaccat caaaatcgtc agtccaacca ttacgacaaa
    19861 agctaccaat gcaaatggtg gaaaagagat cttagcttta ccaaatcaaa aggttgtcga
    19921 atgggttcaa gttaatgggt tagcaatcgg tcaaatttac aaactgattg ttcaaggcta
    19981 cttaacacca gacggcaccc catttgaagg aacacatgcg gaaaaagtct ttatggctga
    20041 taaagaaatg atggaattct tgtttgaatt catcgttgac ggtcgcaacc ttgcaggaaa
    20101 aggattgagt tttgcggaat ggctccaagc caaaacagaa gatgaaaaag aaacgtttga
    20161 agaagtcgca aaacataacg tcgatctaac aaacaaagat caaacggttc aatttgtgga
    20221 ggcaccaaaa ccaccgcaac cagtaaaacc aaacgaacca caacctgtaa aagaagtgaa
    20281 acaagctgca atggtaccta tgttgccaaa aacaggctct gaagacaatg tattatggat
    20341 catgattggt atgatctttg tccttgcggc aggattgatt ggttatgatt tgtataaagt
    20401 cagagactaa agaggacgaa caagcggtta ggcaactagc cgcttgttta gtttttagaa
    20461 agaatttttt atcaaaggca aataaattat tgtgttgagt agtaaatgat catgaggata
    20521 caccaagaaa tactgagtgt aataaataag aatttaagga tccaaaagag atagaatata
    20581 attatgtgat aatacagttt ctttttttct atagaagaaa tcacgtttag ctactcctaa
    20641 ttg
//
LOCUS       DACENJ010000027        19907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.027, whole genome shotgun sequence.
ACCESSION   DACENJ010000027 DACENJ010000000
VERSION     DACENJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.027"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..727
                     /locus_tag="IW748_000752"
     CDS             <1..727
                     /locus_tag="IW748_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1103678.1"
                     /translation="IAIIMDGNGRWAQNRRLPRVAGHKEGMETVKKVTKKASRLGVKV
                     LTLYAFSTENWKRPKDEVSFLMQLPVDFFDTFVPELIKENVKVHVMGYENVLPEHTQD
                     AVRRAIEQTKNNTGMVLNFALNYGSRAEIVTAVQEIAEEVAKGEIHAEEIDDELIAKH
                     LMTGFLPKELQDPELMIRTSGEERISNFLLWQIAYSELYFTKALWPDFDGAHLEEAIA
                     SYQNRDRRFGGVKKTEDEKGDQS"
     gene            724..1524
                     /locus_tag="IW748_000753"
     CDS             724..1524
                     /locus_tag="IW748_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1103679.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1685..2953
                     /gene="rseP"
                     /locus_tag="IW748_000754"
     CDS             1685..2953
                     /gene="rseP"
                     /locus_tag="IW748_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1103680.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3021..4730
                     /locus_tag="IW748_000755"
     CDS             3021..4730
                     /locus_tag="IW748_000755"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1103681.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            4942..9294
                     /locus_tag="IW748_000756"
     CDS             4942..9294
                     /locus_tag="IW748_000756"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1103682.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9438..9911
                     /gene="rimP"
                     /locus_tag="IW748_000757"
     CDS             9438..9911
                     /gene="rimP"
                     /locus_tag="IW748_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1103683.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            9935..11110
                     /gene="nusA"
                     /locus_tag="IW748_000758"
     CDS             9935..11110
                     /gene="nusA"
                     /locus_tag="IW748_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1103684.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11132..11425
                     /locus_tag="IW748_000759"
     CDS             11132..11425
                     /locus_tag="IW748_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1103685.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11422..11733
                     /locus_tag="IW748_000760"
     CDS             11422..11733
                     /locus_tag="IW748_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1103686.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11746..14052
                     /gene="infB"
                     /locus_tag="IW748_000761"
     CDS             11746..14052
                     /gene="infB"
                     /locus_tag="IW748_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1103687.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14079..14426
                     /gene="rbfA"
                     /locus_tag="IW748_000762"
     CDS             14079..14426
                     /gene="rbfA"
                     /locus_tag="IW748_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1103688.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14534..14755)
                     /locus_tag="IW748_000763"
     CDS             complement(14534..14755)
                     /locus_tag="IW748_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1103689.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15203..17566
                     /locus_tag="IW748_000764"
     CDS             15203..17566
                     /locus_tag="IW748_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ1103690.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            17934..18857
                     /gene="truB"
                     /locus_tag="IW748_000765"
     CDS             17934..18857
                     /gene="truB"
                     /locus_tag="IW748_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1103691.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18861..19802
                     /gene="ribF"
                     /locus_tag="IW748_000766"
     CDS             18861..19802
                     /gene="ribF"
                     /locus_tag="IW748_000766"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1103692.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6825 a         3497 c         4378 g         5207 t
ORIGIN      
        1 cattgccatc atcatggacg gcaatggtcg ttgggcgcaa aaccgtcgtt tgccacgagt
       61 tgctggccat aaagaaggca tggaaacagt aaagaaagtc acaaaaaaag catcaaggct
      121 tggagtgaaa gtgttgacgc tttatgcctt ttcgactgaa aactggaagc gaccaaaaga
      181 tgaagtaagt tttctcatgc aactacctgt cgacttcttt gatacgttcg taccagaatt
      241 gattaaagaa aatgtcaaag ttcatgtcat gggttatgaa aacgtattgc cagaacatac
      301 gcaagatgct gtaagacgtg caattgagca aacaaaaaac aatactggaa tggtattgaa
      361 ttttgctttg aattatggaa gtagagcaga gatcgtgact gctgtccaag aaattgccga
      421 agaagtagca aaaggagaaa tacacgcaga agaaatcgat gatgaattga ttgctaagca
      481 tttaatgaca ggtttcttgc ctaaagagtt acaagatcct gagctgatga tccgtacaag
      541 tggagaagag cgaattagta attttctgtt atggcagatc gcatatagtg agctgtattt
      601 tacaaaagcg ctttggcctg attttgatgg tgcgcatctt gaagaagcaa tcgcctctta
      661 tcaaaatcga gaccgtcgat tcggcggtgt aaaaaaaaca gaagatgaga aaggggatca
      721 gtcatgaaac aacgtgtgat cacagcagct gtggcgctag tcttgtttat tccgattata
      781 tggatcggag gcatagcggt tgagcttaca gcagcaatct tagcggtagt aggagtttac
      841 gagctgttcc ggatgaaagg attgacactc ttaagttttg aaggagtttt atccgccgtt
      901 ggtgcagtgt tacttgtttt gccaaaagaa agatggtttt tctttcttcc agagaatgta
      961 agtacattta tgttatttta tcttatagtt atgattcttt taggaacatc agttatttct
     1021 aaaaatacgt atacgattga tgaagcaggt tttcctgtgc ttgtcagctt gtatgtggga
     1081 ataggtttcc agaattttgt caatgcacga acagaaagct tggctgtttt gatatttggt
     1141 ctatttattg tttgggcaac tgatatcgga gcatatatgg ttggtcgacg ttatggtcag
     1201 cataagttaa gtcctgatat ttcgcccaac aaaacgattg aaggtgcgct cggtggtatc
     1261 ggtagtgctg ttgtagtcgc atttctttat ttattgctat acccagcaaa agaactattc
     1321 aatcatggta tgtttgtcat gcttctgatg acgattattt tttcagcagt cggacaattt
     1381 ggtgatttgg tcgaatctgc aatcaaacgt cattatgatg tgaaagactc gggaaatatc
     1441 ttaccaggcc atggtggaat cttagaccgt ttcgacagtt tgctgtttgt atttcctatc
     1501 atgcatttat ttggcattat ttagtaagta tacctgcgtt tctttgtgaa tttcataaaa
     1561 gacgcaggtt tttatttttc attatattga tactattggt gtctttggta tactatggta
     1621 aaattatagt gttttatcgt tgttttataa aaaacacaaa aaacaagtaa aggagaaaaa
     1681 agctatgaaa acgattctga catttatcat cgtttttggt atattagtga ttgttcatga
     1741 gtttggtcat ttcttctttg cgaaacgatc aggaatcctc gtccgcgaat ttgctatcgg
     1801 tatgggtccg aaaatttatg ggcatcaagc aaaagacggg acaacatata cgcttcgcct
     1861 attacctatt ggcggttatg ttcgaatggc aggcaatggg gatgatgaaa cagaaatggc
     1921 accaggaatg cctctttctc ttttactaaa ttctgacggc atcgtagaga agataaattt
     1981 aagtaagaaa atccaattga caaatgctat cccgatggaa ttgagccgtt atgatttaga
     2041 agatgagctg actatcactg gttatgtgaa tggagacgaa acagaagtag taacctaccc
     2101 tgttgatcac gatgcgacga ttattgaaaa cgacggaaca gaaatcagaa ttgcgccaaa
     2161 agacgtacag ttccaatcgg ctaaattgtg gcagcgtatg ctgaccaact ttgctggacc
     2221 catgaataac tttattttgg caatcgtgtt attcatcatt ctggcattta tgcaaggtgg
     2281 tgttcaagtg acgaacacta atcgcgtagg agagatcatg cctaatggag ctgcggcaga
     2341 agcaggctta aaagaaaatg atgaggtagt cagtgtggac ggaaaagaaa tccattcatg
     2401 gaatgatctg acaacagtca ttacgaaaaa tccaggtaaa acattagact ttaagatcga
     2461 acgagaaggt caggtgcaat ctgtcgacgt gacaccgaaa tctgtagagt caaatggcga
     2521 aaaagttggc caacttggta taaaagcccc aatgaataca ggttttatgg ataagatcat
     2581 tggagggacg cgtcaagctt tcagcggttc attggaaatt ttcaaagcct taggatcact
     2641 atttacaggt ttcagtttag ataaattagg cggacctgtc atgatgtacc aattgtcttc
     2701 agaagcagca aatcaaggtg taacaactgt tattagtttg atggcgcttc tttcaatgaa
     2761 tctcggaatc gtcaatctgc ttccgattcc tgccttagat ggcgggaaat tagtcttgaa
     2821 tatttttgaa gggattcgcg ggaaaccatt aagtcaagaa aaagaaggaa tattgacatt
     2881 agctggcttt ggatttttaa tgttgttgat ggtgctagtt acatggaacg atattcaacg
     2941 attctttttc tagaaaaagt tgacaaatag ggtataataa caaacgataa ctattttgag
     3001 gaaataaagg agtatataga atgagacaat caaaaatgtt gattccaaca ttacgcgaag
     3061 ttccaaatga tgcagaagta ttaagccatc aaatcttgct aagagctgga tatatccgcc
     3121 aagtatcagc gggtatttat tcatatctgc cattagcaaa tcgtgtattg gaaaaactga
     3181 aacgcattat gcgggaagaa tttgaaaaaa tcggagcggt agaaatgctg atgcctgcca
     3241 tcttgccagc agaactttgg gaagaatccg ggcgttacga aacatatgga ccaaacttgt
     3301 atcgtttcaa agaccgcaat gatcgaaaaa tgatccttgg accaactcac gaagaaactt
     3361 ttactgattt gatgcgtaat gaaatcaatt catataagaa attaccttta aatctttatc
     3421 aaatccaacc taaatatcgt gacgaaaaac gacctcgctt cggtttgctt cgcggaagag
     3481 agtttatcat gaaagatgca tattctttcc atgcaacgga agaaagtttg gacgagacat
     3541 ataaagatta cgaaaaagcc tataccgaag tattcaagcg ttgtggctta gaatttcgct
     3601 cgatcatcgg agacggcggc gcaatgggcg ggaaagactc aaaggaattc atggcgatct
     3661 cagaaatcgg ggaggatacg atctgttatt cgactgaaag tgattatgct gcaaacttgg
     3721 agatggctac aagctactat gtatcaaaaa aatcgcatga aacacagctt gaattggaga
     3781 aagtagctac acctaacgta aaatcaattg acgaagtagc agatttcttt tctgttgaac
     3841 ctcaaaaaat catcaagtct gtattgttca ttgcggatga acaacctgtc cttgttttgg
     3901 tacgtggaga ccatgaagtc aatgacgtaa aactgaaaaa ctttttatct gctgactttt
     3961 tagaagaagc aacagaagaa gaagcacaaa aatatctagg tgctgagttt ggttctgtcg
     4021 gaccagtcgg tgtttcagat gacgtaaaag tatatgcaga ccgttatgtc caagatttag
     4081 cgaatgccat tactggagcg aacgaaacag gattccacta taaaaatgtg aatccagaac
     4141 gcgatttcac tgtgctttct tatgaagatc ttcgttttgt acaagaagga gatccttcac
     4201 cagacaacaa cggtgttctt gcctttactc gaggaatcga aatcggacat atattcaaaa
     4261 tcggcactcg ttacagtgaa tctatgggag caactgtact agacgaaaat ggacgagaaa
     4321 aatttgtgat tatgggctgt tatggtatcg gtgtaagtcg tttattatca gcaatcgtag
     4381 aacaacattc agatgaacaa ggaatcaatt ggccaaaagg aattgcgcca tttgatctgc
     4441 atatcgtcca aatgaatcta aaagacgaat accaaacaag cctgacgaat gaactagaag
     4501 aagcgatgac aaaagctggc tatgaagtac tggttgatga cagaaatgaa cgcgcaggag
     4561 tgaaattcgc ggattctgat ttgattggct gtccaatcag aatcactgtc ggtaaaaaag
     4621 cagtggatgg aatcgtagaa gtgaaaatca agaaaactgg cgaaatggtt gaagtacgta
     4681 aagaagaatt ggcgaatact ttaccaatcc tcttgaacca aggcgaataa taagcgttaa
     4741 aacacagaaa ataaaaatag ctgaatcagc tgtttttgac aatctgatga ttcgctttta
     4801 aaattggaac tgcgatttga atcgcagttc caatttttta atgtaaaact cagaaaaaac
     4861 gagtatacta aaaggtactg aaaaagaaga ggataagatg cgatcaattg cattgtttct
     4921 catcagaaag ggcgtatatc tttgtcagaa aaacgagatt tatttgaaaa actgttggaa
     4981 caaatccaac tagaggaagc agaaaaaaat catccactgc ttactgctgg tgaaatggat
     5041 agtgtagtgg tccatcgtaa atcgcgttta tgggaattta cgctacattt ttccaaaatt
     5101 ttgcctatta tgctatatcg gtcattgact cagcatctga ctatcgcttt taaagatatt
     5161 gcacaagtca aactgaatat cttagcagat gaccagacat tcgatgaaca gctcttgcaa
     5221 gattactggg cccaagcttt ggaaaatcag caatgcgata caccactagc acagcaagta
     5281 ttgaaaacgc aggtacctgt gataaaagat cgtaaagtca tcttgccaat cgacagtaca
     5341 ggagctattt cttatttgaa gcaacaatac ttgccattag tagaagagtt gttcgtcagt
     5401 tacggattcc caaaatttcg tatcgaacca gaagtggatg agcagcaagc ggaacgtgta
     5461 ttgaaacttt ttgaagaacg caaacaagaa caggcagaag cctttatgaa gcaagcagct
     5521 gaaagtctag ttgtccacga acaaaagaaa aaggaaagaa aagagcaatc gccagctctt
     5581 gaaggaccca tccagctagg acgaaatatt ccagccgatg aaccaacgac accaatgatc
     5641 agtatcgttg aagaagaacg aagagtcaca cttgaggggt acgtattcga caaagaagtc
     5701 agagaactac gttcaaaacg caagatcttg actttgaaaa tcacagatta tacttcatca
     5761 tttatcgtca aaaaattctc gaacggcgaa aaagatgaac aagtatttga tgcgatcagt
     5821 gtcggtagct ggttaaaggt acgaggaagt attcaagaag atacgtttgt ccgtgatttg
     5881 gtcatgaatg cgcaagatat catagaagtc aaacatacac ctcgcaaaga ttatgctcct
     5941 gaaggggaaa aacgagtaga gcttcatgtt catagcaata tgagtacgat ggatgcaaca
     6001 aacagcatct ctgatcttgt ggcacaagca ggaaaatggg gacaccgagc tatcgccatt
     6061 acagaccatg gtggtgcgca agcctttccg gaagcacata gtgctggaaa aaaagcaggt
     6121 gtgaaaatct tatacggcgt ggaagcgaat gtcgtagatg atggtgtccc aattgcttat
     6181 aatgatgcac atgaagcatt gagtgaagcc acatacgtcg tatttgacgt ggaaacgaca
     6241 ggattgtctg cagtatatga cacgatcatc gagctagccg cagtcaaaat gtataaagga
     6301 aatgtgatcg aaagctttga tgaatttatc gatccaggac atccattgtc acgaacaacg
     6361 gtagatctga ctggtatcac ggatgaaatg gttcgagggt caaagtcgga agaagaagta
     6421 ttgcggatgt tccttgaatt ttcaaaagat acaattttgg tagcccacaa cgcggctttt
     6481 gatatggggt tcttgaatac tagctacgca aggtatggta ttcctgaagc ggccaatcca
     6541 gtcattgata cgttggaatt agctagatat ctgtatcctc aattcaaacg tttcggatta
     6601 ggtgtgttat ctaaaaagtt tggtgttagc ttggaacagc atcaccgagc aatctatgat
     6661 gctgaagcaa ctggtcactt agcatggatc tttgtgaaag aggcaatgga caatcataac
     6721 atgctttatc atgatcagct aaatgagcat atcggagaag gagattctta caaaagggct
     6781 cgacctttcc atgtaacgat tttagcaaaa aatcaggcag gactgaaaga tttgttcaaa
     6841 ctgatttcaa tgtcgaatgt cgaatacttc gaacgcgtcc cacgaatacc acgctcccaa
     6901 ttgaaaaaga tgcgtgaaaa tctattgatc ggctcagctt gtgataaggg agaaatattc
     6961 gaagccatga tgcaaaaagg ggtagaagag gcaagaaacc gagcaaaatt ctatgattat
     7021 attgaagtta tgccaaaagc ggtctatgcg cctttgatcg agcaagaatt ggtaaaaaat
     7081 gaacatgatt tggaagaaat tatccaaaat ttggtagaaa tcggaaaaag tttggataaa
     7141 atcgttgttg caacaggtaa tgtacattat ctgaacgaag aagatgccat ttaccgaaaa
     7201 attttgatca attctatggg cggagcgaat ccgttgaatc gtcatagttt gccggatgtc
     7261 catttccgaa caaccgatga gatgctaact gcatttcact ttttagggga agaaacagcg
     7321 aaagagatcg tagtagaaaa tacgaataaa atcgcagata tctgcgaaga agtgattcca
     7381 gtcaaagatg aactttatac accgaagatt ccaggttcag aagatgaaat atcagaattg
     7441 agttacacaa aagcaaaaca aatgtacgga gatccgttac ctgagatcat tcaaaaacga
     7501 ttagaaaaag agttgaattc tattaatggt aatggcttct cggttattta tctgatcgcc
     7561 caaaaactag tgcataaaag taatgaagat ggctatctag taggttcacg tggctctgtt
     7621 ggctctagct ttgttgcgac gatgacagga atcactgaag ttaatccatt agcccctcat
     7681 tattattgtc cagagtgcca atattccgag ttttttgaag atggtacgta tggttctgga
     7741 tttgacatgc cagaaaaaca atgtccaaaa tgtggtgcac gcttaaacaa agacggtcat
     7801 gatattccat ttgagacatt tttaggattc catggagaca aagtgcccga tattgatttg
     7861 aacttctcag gtgattatca agcagaagcc cataactata cgaaagtatt gtttggcgaa
     7921 gactatgttt accgtgccgg aacgattggt acagtcgccg acaaaacagc atatggctat
     7981 gtaaaaggat acgaaaggga caataatctg cagtttcgaa gtgcagaagt cgacagatta
     8041 gcaaaaggcg ctactggagt caaacggaca accggccagc atccaggggg gatcatcgtt
     8101 attcccgatt atatggatgt ctatgatttt acgccgatcc aatatccagc agacgatcaa
     8161 aattcagaat ggaaaacgac tcattttgat ttccactcga ttcacgataa tgtattgaaa
     8221 ttagatattc tagggcatga tgacccaact gttattcgga tgcttcaaga tttatcaggg
     8281 atcgacccac aaacgatccc tacggatgac ccggaagtga tgcggatatt tgccggtcct
     8341 gaagtacttg gagtaagtca ggaacaaatt tattccaaaa cagggacatt aggtattcca
     8401 gaattcggca ctcgttttgt tcgagggatg ttagaagaaa cgcatcctac aacttttgca
     8461 gaactactgc aaatttccgg actatctcat ggtacagacg tatggttagg aaatgcggaa
     8521 gaattgattc gacgaggaga tgcgacactg gcggaagtaa tcggctgtcg tgatgatatt
     8581 atggtttatt tgatccatgc tggattagat agcgggatgg catttaaaat catggaaact
     8641 gttcgtaaag gacaatggaa caagatccct gatgaattgc gagaaaccta tctatcggcg
     8701 atgaaagaaa ataatgtgcc cgactggtat atcgattctt gttcaaaaat caaatatatg
     8761 tttccgaaag cccatgctgc agcttacgta ttgatggctc ttcgtgtcgc ttatttcaag
     8821 gtttatttcc ctattttgta ttactgtgca tacttttctg tacgtgcaga cgactttgat
     8881 ctagtctcca tgtgcaaagg aaaagatgct gtgaaacaag caatgaaaga aatcacggac
     8941 aagggattag atgcttccgt aaaagaaaag aatcaactga cagtgctgga gttagccaat
     9001 gaaatgctgg aacgaggatt caaatttgga atgattgatt tgtataaatc ggatgccgtt
     9061 aactttgtta ttgaaggtga tacgttgatc gcaccattta gagctgtccc aagtttggga
     9121 accaacgtgg cgaaacaaat cgttgaagca agaaaagacg gtccattttt atccaaagaa
     9181 gatcttgcca cccgcggtaa agtttcaaaa acattgatcg aatatatgaa cgacaatggc
     9241 gtattgaaag atctaccaga tgaaaatcaa ttatcgttat tcgatatgct ataagcatct
     9301 gttttcctgt ctgcttgtaa ttttaagcag agtatggtat agttcttata ggtaatgata
     9361 ctaactgtga gtgagcggat ttttcgctca ctctttttaa tggaattacg cacttaattt
     9421 ttaaggaggc gaaacttttg agtagcgtcg ttgaaacagt cacagaaatg gtgacaccga
     9481 ttttagatga acaaaatttt gaacttgtcg aagtcgaatt tgtaaaagaa ggaaaaagct
     9541 ggttcttacg agtatttatt gacaaagaag gcggtatcga tatcgaagaa tgcgcctttg
     9601 tcagtgagaa actgagtgaa aaacttgatg cagcagatcc agatccgatt cctcaagctt
     9661 atttcctgga ggtatcctcc ccgggagcag agcggccatt gaaaaaagaa agcgactatg
     9721 aaaaggcact tggtgattat gtccatgtct ccttatacca accagtagat ggcgaaaaac
     9781 aatatgaagg atttcttcaa tcatttgatg ctgaacagct tacattgaaa atacgcataa
     9841 aaacacgaga aaaagaattg acttttgacc gaaaaaatat tgcaaaagct cgcttagcta
     9901 tccaatttta atcacggaga ggaatcagaa aaaaatgagt aaagaaatgt taaatgcgtt
     9961 agacgcttta gaagctgaaa aagggatctc aaaagaaatc gtgatcgatg cattagaagc
    10021 agcattggtt tctgcataca aacgtcacta tggccaagca caaaacgtcg aagtggaatt
    10081 tgatcagaaa aaaggaaaga tccatgtcta tgcggtaaaa gaagtaacgg aagaagtgat
    10141 ggattcacag ttggaagtct cattgaaaga tgcactttta atcaatccag catatgagat
    10201 cggtgatacg atccgttttg aagtaacacc aaaagatttt ggacggatcg ctgcgcaaac
    10261 ggctaaacaa gttattttac agcgtgtacg tgaagcagaa agaacgatta tttataacga
    10321 attcagtgct tatgaaaaag acatcatgca aggaatcgtc gaacgccagg ataaacgtta
    10381 tatttatgtc aatcttggta aaattgaggc agttctttct aaacaagatc aaatgccaaa
    10441 tgaattttat caaccacatg atcgaatcaa agtttatgtt tcccgagtag aaaatacgtc
    10501 taaaggtccg caagtatttg ttagccgcag ccatccagac ctattgcgtc gcttatttga
    10561 acaagaagta ccagaagtct atgatggaat cgttgaaatt gtcagcatcg cccgtgaagc
    10621 tggcgatcgt tcaaaagtgg cagttcgctc aaatgatcca aatatcgatg cagtcggtac
    10681 atgtgtcggg ccaaagggac aacgagttca agcaatcgtc aatgaattaa aaggcgaaaa
    10741 catggatatc gtcgaatgga atgaagatcc agctgtattc atcgcaaatg cattgaatcc
    10801 tgcacaagtc atggatgtga tcttcgatga gacaaattca aaggcctgca cagttgtcgt
    10861 tccagactac caattatcgc tagcaatcgg taaacgcgga caaaatgcac gtttagctgc
    10921 taaattgact aaccataaaa tcgatatcaa atcagaatct gacatgacgg aattttatga
    10981 aaatcaagaa caacaaaaag aaacagaaga actgcatgat gaagcaatcg ttcagtcaga
    11041 tttaacagac gatgaatatg aaacaattgc attcaacaat gaaacggtcg aagaaaaacc
    11101 agaagcttaa ctttagtaag gaggcaaaga gatgaaacaa aggaaaattc ctttacgcaa
    11161 atccgtggtt tcaggtgaaa tgaaacctaa aaaagagatg gtccgaatca cccgttcaaa
    11221 agaaggggaa gtatcgatcg atccaactgg aaaaatgcct ggacgaggag cgtatgtctc
    11281 acttgagccg gaagaagtac aacaagcttg ggataaaaaa attctcgatc gagtactcga
    11341 aacaacatta acagatgagt tttatcaaga acttcttgac tatgtcactc accaaaaagc
    11401 acgtaaagag ttatttggaa aatgaataca gttaatcggc aaaaagccat gaatctcatc
    11461 ggtctagcga tgcgcgcggg aaaactaatc acgggtgaag aactgaccat tgctgaaatc
    11521 cgcaaacaaa aagcaaaatt ggtgtttgtg gcaaccgatg caagtgagaa tacaagaaaa
    11581 aagataaaag ataagagttc atattatgaa gttccttgct ttgagctatt ttctgaagca
    11641 gaaatcacgc agatgatcgg aaaacctcgc aaggtattcg gaataatgga tgccggattt
    11701 gcgaagaaga ccaaggagct aattgaaggt taggaaggtg attgcatggg caacaaaaga
    11761 atctatgaat tagcaaaaga actcaaaaaa tctagtaaag acgtcgtgga aaaagcacaa
    11821 caacttggca tcgatgtgaa aaaccatatg ggtgcgatta ctagcagtga tgagaagaaa
    11881 ttgcaacaag cgttcaaaaa cccaagcacg aaacaacaag ctgcacaaca agcaagcaga
    11941 aaaccagcca atcaacagcc gaacgatcaa cgtaaaaaag aaacaacgaa caatcaacaa
    12001 aaaaataaaa ataaccgcag ttatcaagat cgcggtcaag ggagcggtca agtgaatcaa
    12061 ggaaaaaaac caacaacaaa ccaaaataat acgggaaata ccagcagcca aaatcgtcaa
    12121 ggaacgagcc aaggcggcaa tcaaaaccgt actaacaata acaacaaacc aaacaataac
    12181 aatcgcggca aatttaataa taataaccgt aatcgttata ataaaaaagg taaaaaaggc
    12241 aaacagcaac aatcaactaa accagctgta ccaccacgca aattccgtga attgccagat
    12301 gtattagagt atactgaagg tatgaacgta gcagatattg cgaaaaaaat tcatcgtgag
    12361 ccagcagaaa tcattaaaaa attatttatg cttggtgtca tggtcaatca aaaccaagca
    12421 ctggataaag atacgattga actgttagcg acggactatg gcatggaacc acaagaaaaa
    12481 gtccaagttg acattgctga tatcgataaa ttcttcgagg cagaagaagt cgatccagat
    12541 aaattagtct cacgtccgcc agtagtaacg attatgggac acgtagacca cgggaaaaca
    12601 accttattgg atacattgcg ccattcacgt gtaacaagcg gcgaagccgg cggaatcaca
    12661 cagcatatcg gtgcctacca gatcgatatt gatggtaaac caatcacttt cttggataca
    12721 ccaggacatg cggcctttac aagtatgcgt gcacgtggag caagtatcac agatatcacg
    12781 atcttagttg ttgctgcaga tgatggcgtc atgccacaaa cagttgaagc aatcaaccat
    12841 gccaaagctg caggcgttcc aatcatcgtc gctgtaaata aaattgataa accaggtgca
    12901 aatcctcagc atgttatgca agaattaagt gagtatgaat taattcctga agcatggggt
    12961 ggagaaacga tttttgttga gatctcagcg aaattcggcc aaaacatcga agaattgctg
    13021 gaaatgatcc tgttagtcgc agaagtagaa gatttaaaag cagatccaac acaacgcgca
    13081 atcggaacag tgatcgaagc acgtcttgat aaaggtaaag gtcctgtgtc aaccttactt
    13141 gtccaagaag gaacacttca cgttggtgac ccaatcgttg tcggtaatac ttacggacgt
    13201 gtccgtgtaa tgacaaacga tcttggccgt cgcgataaag aagcaggacc tgcaacgcca
    13261 gtagaaatca ctggcttgaa tgatgtacct caagcaggtg accgtttcgt agtcttcgaa
    13321 gacgaaaaga cagcacgtgc agcgggtgaa gaacgcgcaa aacgtgcatt attggaaaac
    13381 cgtgctgcca caagccgtgt gactttggac aacttatttg aaagcctgaa agaaggcgaa
    13441 ttgaaagaag ttaacgtaat catcaaagca gatgtacaag gttcagcaga agcactggct
    13501 gcatcattga agaagattga tgtggaaggt gtccgtgtaa aaatcgtcca ttcagctgtc
    13561 ggagcaatca atgaaagtga tgtaacctta gcagcagcca gcaacgctat tatcatcggt
    13621 ttcaacgttc gtcctacacc gcaggcaaaa gtgcaagctg atactgaaga agtcgatatt
    13681 cgtcttcacc gtatcatcta taaagccatc gaagaaatcg aaactgcgat gaaagggatg
    13741 ctagaccctg aatttgaaga aaaaatcacc ggtcaaatga tcgttcgtga aaccttcaaa
    13801 gtttctaaag ttggtacgat tgcaggtgcc tatgtaacag aaggatatat tcgtagagat
    13861 agcggtgtcc gtgtaatccg tgacggaatc gtaatctatg aaggccaact tgcaagtctg
    13921 aaacgattca aagatgacgt gaaagaagtc aaaatgggtt atgaatgtgg tgcgatgatt
    13981 gaaaaattca atgacatcaa agtagatgac gtaattgaag gatttatcat ggaagaagtc
    14041 aaaaatgact aaaaactgat aaaaggaggc caatcattat ggctaactac cgtgatcgcc
    14101 gagtcggtca agaaatctta aaagaagtca atgatatttt gcgcaaaaag gtccgtgacc
    14161 ctcgtgtgga gaacgtaacg atcacagatg tgaaagttac gggggactta cagcaagcaa
    14221 cgatctatta cagcatttta tctgacttag catcagataa gaaaaaagca caagatggat
    14281 tgaataaagc atcaggattg attcgtcgag agttagggca caatctaagt atttataaga
    14341 cacctgaatt gttctttgaa cttgatgaat cagttgtcta tgggaaccat atcgatgaat
    14401 tgcttcgcga cttgaacaaa gactaatcaa aaaatcccgc aaatcctgtg aaatcagggg
    14461 ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg actttagtct
    14521 ggtagcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg actttctttt
    14581 ttctatagta tataaatttt tgcatataga taaagatcaa gataccgata acggaagcta
    14641 gattgtaatc tattttagtg acagtcaggt atagccaagt aactagaaga tcagtggaac
    14701 ctgcaccata aaagtttgag tttgtcggtc cgttgacagt taaaagaaaa atcacgttga
    14761 agttattgat atttccgatt acgccgcttc gatttggtca cgatccaaat tttggataat
    14821 ccctgtagaa attagcatag tagctgggat accaattcac atattgacaa agataacaga
    14881 gacttttaca taaaacggat caatcaggaa aggaattgct cgatccagcc ccagttttcc
    14941 aacaatgtgt taactggcct agcgtcattc agcaaattat gcatgatgag gagcgaaatg
    15001 aactgcggaa cagccattgt gataacgaca agttttttgg atcaatgtaa aaaacgtcag
    15061 cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt ggctagatat aaaatccaaa
    15121 caaatgaaca gaagtgctta ttaggtttca attgggagta tctgcgctag aataagagta
    15181 ataatgaaaa ggagtgttat gtatggatta ttctagtaaa gaatactttg ataaaatgac
    15241 ggcttggtgg cgtgctgcta attatctttc tgttgggcaa ctttatctta aagataaccc
    15301 cttactgaga cgtacactga aaccagaaga tgtcaaaaaa catccaatcg gtcattgggg
    15361 aacgattcct ggccaaaatt ttatttatgt ccatttgaac cgagtcatca ataaatatga
    15421 tttgaacatg ttttatatcg aagggccagg acacggcgga caagtcatgg tatcgaatgc
    15481 ttatttagat ggaagttaca cagagatcta tccagaagta acggaagatg agacaggaat
    15541 gcaaaaatta ttcaagcgat tttcatttcc tggaggcatt gcttctcatg cagcaccaga
    15601 aacaccaggt tctatccatg aaggcggaga gcttggatac tctctttcac atgcagtcgg
    15661 agctgtatta gacaatcctg aagtaatctc tgctgtcgtg attggtgatg gtgaagcaga
    15721 aaccggtcct ttagctggaa gctggttctc aaatgtcttt atcaatccag tgatagatgg
    15781 agctgtttta ccgatccttc atttaaatgg tgcaaaaata gccaacccaa cgatcttggc
    15841 acgtaaatca gatggagaat tagcaaatta ctttaacgga cttggctggg agcctttctt
    15901 tattgaagga aatgatccag aaaaattaaa tcctgttatg gctgaaaaaa tggatcaagc
    15961 aatcgagaaa atcaaatcca tccaaaaaga agctcgcctg aagacagcaa cagatgtcgt
    16021 gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa ggatggaccg gtccaaaaga
    16081 atgggacggg gaaccaattg aaggaacctt ccgagcacac caagtaccga ttcctgtaga
    16141 tcaagaacat atggatcatg cagatgcttt actccgttgg ctgaaatcct atgaaccaga
    16201 aaaattattt gatgcacaag gacgtatttt agaagaaatc agagaaattg cgcctactgg
    16261 tgaccaaaga atggcaaaaa atcctatcac caatggtgga atcgatccaa gaccgcttat
    16321 tatgccagat tggaaaaaat atactttgca atttgaaaaa ctaggctccg ttaaagcaga
    16381 agatatgacc gaacttggta aattcgttcg cgagatcatt gagaaaaatc cagaaaattt
    16441 ccgtatattt ggaccagatg aaacaaaatc aaaccgattg aatcaagttt tcaaaacgac
    16501 aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat gaatggcttt cgccatctgg
    16561 tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa gggttcttag aagggtacgt
    16621 tctgactgga cggcatggtt tttttgcaag ttacgaatca ttcttgcgag tggttgactc
    16681 tatgttgact cagcacttca aatggatacg gaaatcacat gacctttctt ggcgaaatga
    16741 ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc acaatggata
    16801 tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg agttcatccg
    16861 tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag cattccactc
    16921 gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat tttactcagc
    16981 agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg cttcgacagc
    17041 aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta acttagaagc
    17101 attagcagct gcaactttgc tgctggaaga attcccaaaa ctaaaaattc ggttcattaa
    17161 tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt tgagcgatga
    17221 agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc acggttatga
    17281 aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga ttcatggcta
    17341 caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg aacttgatcg
    17401 ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag aagattttgc
    17461 ggaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa atggtattga
    17521 tctgccagaa gtattggatt ggaaatggaa aaatcttgat caataaaatg ataaaatcga
    17581 tcccgcaaaa cagaattttt tacattgatt aagaaactga aaaaataaat aaaaaaaacg
    17641 gaaaaaagac tggaagaatg gacagtcttt tttttcgttt ttgggaagtg aaaaccaaac
    17701 tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa atcttccatt
    17761 ctattccctc ctttaaaaaa acataacatg atttctattt acgtgagcat gaattttgtt
    17821 acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg tcagtaattg
    17881 atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa gacatggacg
    17941 ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta ttcaaattaa
    18001 gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca gacgtagacg
    18061 gtgtgttgcc tatttgtatt ggaaaagcca caaaagttat cgagtatttg acagattctg
    18121 ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa gatcgatccg
    18181 gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaagaaaaa atagatgaag
    18241 tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca gcggtaaaag
    18301 tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag cgtcctgtta
    18361 gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac gaagtgacag
    18421 gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc cgcacattag
    18481 ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg acccgaacag
    18541 caagtgccgg tatgtcccag aaagatgctc tgacattaga agaagtagct caagctgtga
    18601 aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtgaaa cgattcaaac
    18661 gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga cttgattata
    18721 ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat aaaggcaaag
    18781 ttgtcagtat ctatcagcca aacccgaaag aaaaaaacaa attaaaacct agtaaagtat
    18841 tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac caatcagaac
    18901 aaattccaga agacgatgtc gttttagtac tgggattttt tgacggcgtg catattggac
    18961 accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg aaattagcat
    19021 tgatgacttt caatcagcat ccatcgattg tctttaaaaa gatcgatcct agcagcgtga
    19081 aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt atagattatt
    19141 tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa tttgttgatc
    19201 aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat acgtatggac
    19261 caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga ttcaaaatcg
    19321 tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt attcgaggat
    19381 tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca tatgaggtag
    19441 aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct acagcaaatg
    19501 tcaaagtaaa gagcacagtt catttgccta aagaaggtgt ttatgtatcg gaaataaaaa
    19561 ttggggaaac ttggtatcca gccatgggtt caattggaca taatgataca ttcggtgaag
    19621 gacgggagct gactgtagaa ttgtatattt tggactttaa tcaagatatc tatggtgaac
    19681 atgtcgcagt acgctggaat caatttttaa gagatcaagt gaaattttct agtgtagagg
    19741 aattgattga acaactcaaa aaggatgaac aagcaacagc tgaatatttt tctcagcaat
    19801 aaaacattat gcataaaatc tcttgtttat gtataactag caatttcaat aaaatgggtg
    19861 ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagcc
//
LOCUS       DACENJ010000028        19830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.028, whole genome shotgun sequence.
ACCESSION   DACENJ010000028 DACENJ010000000
VERSION     DACENJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.028"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(13..996)
                     /locus_tag="IW748_000767"
                     /pseudo
     CDS             complement(13..996)
                     /locus_tag="IW748_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            complement(1001..1648)
                     /locus_tag="IW748_000768"
     CDS             complement(1001..1648)
                     /locus_tag="IW748_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1103693.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="IW748_000769"
     CDS             complement(1733..2365)
                     /locus_tag="IW748_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1103694.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IW748_000770"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IW748_000770"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1103695.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="IW748_000771"
     CDS             complement(2929..3666)
                     /locus_tag="IW748_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1103696.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="IW748_000772"
     CDS             complement(3663..4652)
                     /locus_tag="IW748_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1103697.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4910..5452
                     /locus_tag="IW748_000773"
     CDS             4910..5452
                     /locus_tag="IW748_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1103698.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5469..6554
                     /locus_tag="IW748_000774"
     CDS             5469..6554
                     /locus_tag="IW748_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103699.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6566..7372
                     /locus_tag="IW748_000775"
     CDS             6566..7372
                     /locus_tag="IW748_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103700.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7373..8200
                     /locus_tag="IW748_000776"
     CDS             7373..8200
                     /locus_tag="IW748_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103701.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8197..9270
                     /locus_tag="IW748_000777"
     CDS             8197..9270
                     /locus_tag="IW748_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1103702.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9698..12394
                     /locus_tag="IW748_000778"
     CDS             9698..12394
                     /locus_tag="IW748_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1103703.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12471..12959
                     /locus_tag="IW748_000779"
     CDS             12471..12959
                     /locus_tag="IW748_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1103704.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            12971..13465
                     /locus_tag="IW748_000780"
     CDS             12971..13465
                     /locus_tag="IW748_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1103705.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13486..14295
                     /locus_tag="IW748_000781"
     CDS             13486..14295
                     /locus_tag="IW748_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1103706.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14292..15113
                     /locus_tag="IW748_000782"
     CDS             14292..15113
                     /locus_tag="IW748_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1103707.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15164..17263
                     /locus_tag="IW748_000783"
     CDS             15164..17263
                     /locus_tag="IW748_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1103708.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17260..18378
                     /locus_tag="IW748_000784"
     CDS             17260..18378
                     /locus_tag="IW748_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1103709.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18417..19295
                     /locus_tag="IW748_000785"
                     /pseudo
     CDS             18417..19295
                     /locus_tag="IW748_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19486..19638
                     /locus_tag="IW748_000786"
     CDS             19486..19638
                     /locus_tag="IW748_000786"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103710.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         6049 a         3445 c         4245 g         6091 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagctagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatacgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
     4681 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     4741 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
     4801 gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact aatacgagtg
     4861 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
     4921 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
     4981 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
     5041 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
     5101 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
     5161 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
     5221 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
     5281 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
     5341 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
     5401 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
     5461 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
     5521 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
     5581 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
     5641 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
     5701 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
     5761 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
     5821 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
     5881 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
     5941 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
     6001 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
     6061 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
     6121 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
     6181 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
     6241 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
     6301 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
     6361 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
     6421 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
     6481 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
     6541 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
     6601 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
     6661 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
     6721 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
     6781 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
     6841 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
     6901 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
     6961 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
     7021 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
     7081 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
     7141 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
     7201 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
     7261 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
     7321 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
     7381 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
     7441 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
     7501 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
     7561 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
     7621 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
     7681 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
     7741 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
     7801 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
     7861 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
     7921 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
     7981 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
     8041 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
     8101 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
     8161 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
     8221 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
     8281 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
     8341 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
     8401 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
     8461 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
     8521 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
     8581 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
     8641 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
     8701 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
     8761 tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa attgaatcat
     8821 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
     8881 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
     8941 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
     9001 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
     9061 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
     9121 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
     9181 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
     9241 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
     9301 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
     9361 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
     9421 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
     9481 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
     9541 tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc tatgaaaaaa
     9601 cataaaaatc atattagata aaaactaaat tggtagataa attattttgt cagatatctg
     9661 aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat atagaataga
     9721 catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg aaaaaatgaa
     9781 aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga atatgtcttc
     9841 tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg ccagccatta
     9901 tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc ctgttttgtg
     9961 gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg aagcctttca
    10021 ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta tagccgctgt
    10081 caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg ttggaaaaag
    10141 ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg aaaaagatgc
    10201 accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc tttcggcaac
    10261 actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg atggtttatt
    10321 ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat cgaaagaaaa
    10381 tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg gtgaaaatgt
    10441 aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag gtgaaaatta
    10501 tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa cttttgaaga
    10561 tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg aagcggatgt
    10621 tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat ctcatccctt
    10681 tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg atgtgtatag
    10741 tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc agttaaatat
    10801 gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga aaaatcaaaa
    10861 gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa aaattactca
    10921 gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg aagtacagca
    10981 tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat tgatgattca
    11041 gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga cgacgagtgg
    11101 acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata agacagactt
    11161 aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact tatgtaatca
    11221 gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa gactgcttat
    11281 tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc tgttgcttgc
    11341 tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat gcggcaatta
    11401 tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg tcgaatcgac
    11461 aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg tagatatggg
    11521 ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt tacttcttct
    11581 gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga tgaatgcgcc
    11641 atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc aatactttga
    11701 aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg ggatttcgga
    11761 caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag ttttcacgaa
    11821 ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt ttgacaagac
    11881 aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta atattaatgt
    11941 ttatgacatc ttagacggtg atggagcagc caatttggag catattttga gtcctgaaat
    12001 gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt cgttggaagg
    12061 aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag tggaaacagt
    12121 catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa aactaaatct
    12181 ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag aaaaatctga
    12241 agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt ctagaagtat
    12301 ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc tttcgctgct
    12361 ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg tctaatctgt
    12421 tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca atgtttttaa
    12481 aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt aaccatatga
    12541 agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc agtattcaat
    12601 tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat gagtgtgatt
    12661 ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag atttttgttt
    12721 ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt ttggagaaag
    12781 agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa atagtattgg
    12841 caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc caacaggaat
    12901 taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat tttttttagg
    12961 aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg gacaagtagc
    13021 gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg atgaggcggc
    13081 taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg tcaaagtcat
    13141 tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga aggacatgcg
    13201 cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag ctttcccaac
    13261 ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat cggaagataa
    13321 gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact taaaagaatt
    13381 agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa aacgttcagc
    13441 tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta tgatgcattt
    13501 gtagtcgctt tggccgtttt tattggagtt gctggacatg aaatgttcgg gatgtccatg
    13561 ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga tttgcagacg
    13621 gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa tatcggaatc
    13681 tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc aatcaaaatg
    13741 ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt aggattacag
    13801 ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt tttcaaattt
    13861 gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg gtttgtccac
    13921 tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga tgttgtcaaa
    13981 agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc tggaaatctg
    14041 ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa tattgctgtg
    14101 tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat tgcaattgcc
    14161 gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact aaaaaatata
    14221 tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca agaagaggag
    14281 gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa ttggttcgga
    14341 agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg atgggcggcg
    14401 gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt gaagaagatc
    14461 gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct tgcgcgtcct
    14521 ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct ggttttgacg
    14581 tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga attggcgatt
    14641 cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac ttagccaatg
    14701 ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct aattacttga
    14761 tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt gaaaagctgt
    14821 atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt ctgatcatgg
    14881 taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt tcaatgaagg
    14941 gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga attgtaccgt
    15001 tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc atcaaaatta
    15061 tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca tagtaggaag
    15121 ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta ttaatgaagt
    15181 aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa atgtgactac
    15241 ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga ccttcgatgt
    15301 aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa cgttgacctt
    15361 ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata aacagctctc
    15421 tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc atttgtcaga
    15481 atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat atattcatca
    15541 ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta ataagcgttt
    15601 aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc ttcaagtttc
    15661 agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg tgttgatttc
    15721 tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg ccattgcttt
    15781 tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata tcgaaaaatg
    15841 gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa tgccaaacga
    15901 tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt tcggtatgaa
    15961 tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta atcgaatttg
    16021 gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg agttatgtca
    16081 aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa aatttacagg
    16141 aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta gtctttattt
    16201 tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac tgaatgcttc
    16261 aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc tttttccatc
    16321 tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca atctttgtct
    16381 ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac tgagtcttag
    16441 caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac aaatgactat
    16501 cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga aaacaaccta
    16561 ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt taagtgatgt
    16621 gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag acactacgtt
    16681 gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca ccatggcctt
    16741 ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca cttggggatt
    16801 agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc tgcacaaccc
    16861 tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag acggttcgtg
    16921 gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag actttggctg
    16981 cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc aatatttcgg
    17041 attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa cgttttcttg
    17101 gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat ccgagttgag
    17161 tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa ttcttcacgt
    17221 agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact aaaaaatatt
    17281 acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac aggcgagaaa
    17341 ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg tggcgcaatt
    17401 ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg aataattcgt
    17461 catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt acgtgattta
    17521 atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc tttaggggat
    17581 ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat agtggttaat
    17641 cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc taaaattacg
    17701 atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa ttacgctttg
    17761 gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg ggaacgcatt
    17821 accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg ctattacgtg
    17881 ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg cgagtttaaa
    17941 ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc agaagattat
    18001 tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca tgcgaaaaac
    18061 tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac aagagatcgt
    18121 tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat gccacgacat
    18181 ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt atcggtcact
    18241 ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga tgttgctagt
    18301 gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt cactgatcga
    18361 gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa cctatgatga
    18421 tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt actgaggaat
    18481 taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca atgaagcagg
    18541 tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc ggctgctttg
    18601 gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca acaccaaaac
    18661 ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat attccgattg
    18721 aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt tctggcaaag
    18781 ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt gccttgttca
    18841 atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg atcaagcgac
    18901 atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta gaggggatgg
    18961 cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta ttggctgacg
    19021 attagttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat acaacactga
    19081 tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa cacatgaaga
    19141 aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata ccaccaattg
    19201 atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc ggattagcat
    19261 tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca ttgatactca
    19321 tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa agcgaaaatg
    19381 aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca ctgaaaggaa
    19441 atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag gatattcact
    19501 ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt aatagttata
    19561 gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct tggcttaatg
    19621 tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta attctattat
    19681 agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta tacattttaa
    19741 atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttac ctttaactct
    19801 cgcttctctt gcaaatgtag ttagcatttt
//
LOCUS       DACENJ010000029        19454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.029, whole genome shotgun sequence.
ACCESSION   DACENJ010000029 DACENJ010000000
VERSION     DACENJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.029"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            117..3644
                     /locus_tag="IW748_000787"
     CDS             117..3644
                     /locus_tag="IW748_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1103711.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3641..7363
                     /gene="addA"
                     /locus_tag="IW748_000788"
     CDS             3641..7363
                     /gene="addA"
                     /locus_tag="IW748_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1103712.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7432..7773
                     /locus_tag="IW748_000789"
     CDS             7432..7773
                     /locus_tag="IW748_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1103713.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8082..9125
                     /gene="pheS"
                     /locus_tag="IW748_000790"
     CDS             8082..9125
                     /gene="pheS"
                     /locus_tag="IW748_000790"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1103714.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9130..11550
                     /locus_tag="IW748_000791"
     CDS             9130..11550
                     /locus_tag="IW748_000791"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1103715.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11720..12730)
                     /gene="trpS"
                     /locus_tag="IW748_000792"
     CDS             complement(11720..12730)
                     /gene="trpS"
                     /locus_tag="IW748_000792"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1103716.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12984..13721
                     /locus_tag="IW748_000793"
     CDS             12984..13721
                     /locus_tag="IW748_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103717.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13735..14550
                     /locus_tag="IW748_000794"
     CDS             13735..14550
                     /locus_tag="IW748_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1103718.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14563..15210
                     /locus_tag="IW748_000795"
     CDS             14563..15210
                     /locus_tag="IW748_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1103719.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15226..15882
                     /locus_tag="IW748_000796"
     CDS             15226..15882
                     /locus_tag="IW748_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1103720.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16044..16865
                     /gene="racE"
                     /locus_tag="IW748_000797"
     CDS             16044..16865
                     /gene="racE"
                     /locus_tag="IW748_000797"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1103721.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16868..18223
                     /gene="rph"
                     /locus_tag="IW748_000798"
     CDS             16868..18223
                     /gene="rph"
                     /locus_tag="IW748_000798"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1103722.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18224..18742
                     /locus_tag="IW748_000799"
     CDS             18224..18742
                     /locus_tag="IW748_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1103723.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18849..19349
                     /locus_tag="IW748_000800"
     CDS             18849..19349
                     /locus_tag="IW748_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1103724.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6542 a         3342 c         4178 g         5392 t
ORIGIN      
        1 aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag aacaaatgtt
       61 cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg aaaagcatgt
      121 ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg atcgacgaag
      181 catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt cccaactaca
      241 gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc ggaaaaaaaa
      301 ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg tatttgctgc
      361 aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca atgatcttgc
      421 gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa atcaataaaa
      481 gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga aatattcaac
      541 cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag caacttaaaa
      601 tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa cgtgcactaa
      661 caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat ttcaacaacg
      721 tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa ttactgcagg
      781 caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct tatacagcgg
      841 atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc cgtctttttc
      901 aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca gagcagactc
      961 gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc caaaagaaag
     1021 cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct gaaaatccaa
     1081 ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa gaaaactacc
     1141 gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat atccttcggc
     1201 cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg atggaaaatc
     1261 atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct tatcggctaa
     1321 gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca gaaaccgaac
     1381 ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc actgaaaatg
     1441 tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac tggcagtttg
     1501 tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa gaacagtcaa
     1561 atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa ataaagaaaa
     1621 gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa agtggcgtac
     1681 agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta gaagcggcaa
     1741 gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat gtagtaattt
     1801 atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt ggtctagaaa
     1861 atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat cgtttggaat
     1921 tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat caagtgtttc
     1981 cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta ttaaatcagg
     2041 ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct tttgagcctt
     2101 tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt tatgcacaaa
     2161 gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc acagaatatt
     2221 tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct aattgttatg
     2281 tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta gctttagatg
     2341 aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg aattcgtcat
     2401 ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt cctgtttcga
     2461 tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca atttcacgta
     2521 tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg aaattgaaag
     2581 aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat gaatcattgg
     2641 atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg tccgatgcgc
     2701 agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt cggtttgaga
     2761 tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag acgattcaaa
     2821 aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct cttcagacag
     2881 agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa ttaccgttga
     2941 gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta gcttctgaac
     3001 aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca ttcgatgtca
     3061 cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta gcattgatgg
     3121 atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat tatttacatg
     3181 ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg aaaaaattta
     3241 gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat cattcactgg
     3301 aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat gaataccaaa
     3361 aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta ttcatggcgc
     3421 ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa atcaaactaa
     3481 atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc agaagcatct
     3541 gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa atagataagg
     3601 aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa ttcctttaaa
     3661 accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc aaggagataa
     3721 tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc gaagagtgat
     3781 tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat ttactgaagc
     3841 tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg tcaatagcga
     3901 aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc ccacagcgaa
     3961 tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt acttaattga
     4021 tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga tgaaggagga
     4081 tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat ttttccaatt
     4141 aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg tgttttctct
     4201 ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt taagcgataa
     4261 ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa tccgtccttt
     4321 agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc agttagcaca
     4381 aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc agatcaagaa
     4441 tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt tggaacaact
     4501 aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt ccaatgaagt
     4561 caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa gttattttcc
     4621 tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag tcgaagaaat
     4681 gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg aaaaaggtgt
     4741 gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa aaacgaaaga
     4801 tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag aagtaatggt
     4861 agatgaatat caagacgtga accaattaca agaagccatt ttatactggc taagagaacc
     4921 cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca tctattcttt
     4981 ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa agaaagaagg
     5041 cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc tttcttttac
     5101 gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt atgacgaagc
     5161 agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg agccggaaat
     5221 catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg atgatatact
     5281 ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc aattaattga
     5341 ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag agtacaaaga
     5401 cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga ttttcaaaac
     5461 attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga cagaaattcg
     5521 cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc cgcttgcggc
     5581 tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca ttcgattagc
     5641 taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa acgaagatga
     5701 attagcagct aagctggaac attttggcaa gcaactagag cattggcgag aacttgctcg
     5761 ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat atctagagta
     5821 tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat tggttgatcg
     5881 tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc gtttcattga
     5941 aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag aagataatgc
     6001 tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg tatttttgtt
     6061 agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt ttgaagaaaa
     6121 acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg atacgttacc
     6181 gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa tgcgtaaatt
     6241 gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct ataaaaataa
     6301 agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag tttttgatcc
     6361 tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg gattgatccg
     6421 tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc ttcaacattc
     6481 aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac aaagacagtt
     6541 gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg aaactttatt
     6601 agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt cttcacaaac
     6661 cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag atgatacaca
     6721 agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc aatttcgtta
     6781 tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag tgtcagcagc
     6841 tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga tgccaactac
     6901 agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag acgaaaaagt
     6961 cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac ttggtcaaca
     7021 gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc tgcttcctgc
     7081 tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc acggaatcgt
     7141 cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa cagattttat
     7201 tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc aaggacagct
     7261 aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa atgttttcct
     7321 aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat tgagcaaact
     7381 tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat gatggagcta
     7441 acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat tttaggaaaa
     7501 aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg ctttgttgag
     7561 cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt gaaggaatta
     7621 gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg ttcagaatat
     7681 ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca agtttggagc
     7741 gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct tgtaaactat
     7801 agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta aaccagttca
     7861 gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt tcactttccg
     7921 agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt gcggtgtaag
     7981 tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca ggaatgaggt
     8041 ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta caagcacaat
     8101 tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat ttaaatgaat
     8161 taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa gtattacgtg
     8221 ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca aatgagatcc
     8281 gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa gaagcgttaa
     8341 atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa gtaaaacatg
     8401 gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt gtgggtatgg
     8461 gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc gaacggatga
     8521 atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt tcagacgaga
     8581 tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa catgatttct
     8641 ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat acagatgatg
     8701 cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa aacatcacaa
     8761 tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg gaagatcgtg
     8821 aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa gtagatgtca
     8881 gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga tggatcgaaa
     8941 ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc gatccagaag
     9001 aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg cggtatggag
     9061 tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag ttcaaggggg
     9121 agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt atcgaatgta
     9181 acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga gggagtaacg
     9241 gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg cgtaccacat
     9301 ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga actgtctcaa
     9361 atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc acttcctaac
     9421 tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca agtatcaaat
     9481 ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc gaaagaatac
     9541 gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt ttttccttat
     9601 ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc ggatgcattg
     9661 tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc tacattcgat
     9721 gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt ggaagttgaa
     9781 gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag aatccagcca
     9841 agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat caacaatgta
     9901 gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc atttgactac
     9961 gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga aacattagta
    10021 acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac taacggcaaa
    10081 gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac gaatgaaaca
    10141 acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg tacagcaaaa
    10201 caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa ccatgcgact
    10261 gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg tggggaagtt
    10321 ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt gaagatttca
    10381 cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt aaatgagatt
    10441 tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt agtagtacca
    10501 ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc tcgtatttat
    10561 ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg aagcttgtca
    10621 aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg attgagcgaa
    10681 gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat aaatgaaagt
    10741 aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg aatgaatttg
    10801 atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac agagattgct
    10861 ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca tctgccgcaa
    10921 gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga ttggcagacg
    10981 aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt atttgaacaa
    11041 ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga aatgcatcct
    11101 ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg tcaagtgcat
    11161 ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt tgatttatct
    11221 gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa attcccagca
    11281 gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga attaacagaa
    11341 gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga tgtctatcaa
    11401 ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt cgtcaaccca
    11461 gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga aaaagcgttg
    11521 gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta acgatcaagt
    11581 agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca catctctttt
    11641 tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct ttacagcctg
    11701 cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat tttacttcag
    11761 aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag atttccataa
    11821 cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca aattcagctt
    11881 ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg cggcggtaat
    11941 gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag taggtgaaga
    12001 ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga tcagtataca
    12061 tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata ccgtttccta
    12121 acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt ccttttggtg
    12181 ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag ctttgtacga
    12241 tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca agattcgctt
    12301 tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct gtaggcacgc
    12361 tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc cgaacgcgtc
    12421 ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga atttgtgatt
    12481 ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct aaagcaactt
    12541 gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt gcttgcatgt
    12601 cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact cgggttttta
    12661 gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc gttaaaataa
    12721 tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa tatcttaccg
    12781 tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg ataaaataac
    12841 taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag aaactttaca
    12901 aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga aatcttacta
    12961 aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg gataaatatt
    13021 atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga gaagttgtcg
    13081 ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc aatggattag
    13141 agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat aaaaagacaa
    13201 aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat ttgtacccaa
    13261 ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag caagaccagg
    13321 caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta gataagaaag
    13381 attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc gccagaggtt
    13441 tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg gatccggaaa
    13501 tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg tcaatgattg
    13561 tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc ttcatggctg
    13621 atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat ccaaatgaag
    13681 tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa aaagatgcgc
    13741 aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc actagccgga
    13801 tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc taccaatgaa
    13861 atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat tgagagcaga
    13921 actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat ccttggtgac
    13981 aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt attgaaaaac
    14041 ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa aaaacaggtt
    14101 gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa aggaagccaa
    14161 atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa aggttctaca
    14221 tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt agaaaactat
    14281 gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac ggataatgcg
    14341 attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg aacgtttact
    14401 aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa agctgttaac
    14461 caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca aaaatggttt
    14521 ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga attgatacaa
    14581 acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag tgtcttagca
    14641 cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc aaagaataaa
    14701 gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc attattaatc
    14761 atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga tggattccaa
    14821 gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac agtacgttca
    14881 gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg ttttacctat
    14941 tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat cccgccatta
    15001 ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt agctggttta
    15061 gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt cgatacgtat
    15121 atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt aatggtttat
    15181 cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga ttttagcggc
    15241 gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac tgttgaagtt
    15301 tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt gattcgtttt
    15361 gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat acggaatctt
    15421 ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg cattcagatg
    15481 aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat gttgtcggaa
    15541 attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg attatcaacg
    15601 ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt taaatcaatg
    15661 atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt agcagtaatc
    15721 atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa caccaattat
    15781 gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta cgcgctttct
    15841 ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat tcttttagcg
    15901 gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa aagagatttc
    15961 gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca caatagggag
    16021 gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct atcggattta
    16081 ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa ttaccgaatg
    16141 aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga cccgcggaac
    16201 aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga atcaagatgc
    16261 tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa gctgctcttt
    16321 ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa aaaacacaaa
    16381 ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct tatgaaaaag
    16441 cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc aaatttgttt
    16501 cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg gcagaaacat
    16561 tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc cattatccat
    16621 tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc gattctggag
    16681 cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc aattcaccgc
    16741 aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt ttcgaggaaa
    16801 tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa ttgggaggaa
    16861 aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg agcttcaaac
    16921 aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata caacagttat
    16981 ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag gaacaggatg
    17041 ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc gtagagaaag
    17101 cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg gtcgttcctt
    17161 gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag actgcgatgt
    17221 gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg tggcattgaa
    17281 attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa taaaagaaca
    17341 tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag atttggatta
    17401 ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat ctggaaaatt
    17461 cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt tgaatgaaat
    17521 gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac atgctttgct
    17581 aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca tcattgcgac
    17641 aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg gatatcatgt
    17701 aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca gcacatttga
    17761 agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc cagtcttagc
    17821 agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct attctgcacg
    17881 ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat atgaattgac
    17941 agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat tcgcggcacc
    18001 caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc gtataccatc
    18061 tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga aacaaacagc
    18121 agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag caatgaaaaa
    18181 attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt atttagtagt
    18241 aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt atcgttctga
    18301 agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc cattatggga
    18361 tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac agaaaaaagt
    18421 gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga atgtccgatt
    18481 tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg tgttttttgg
    18541 tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga atcctggtag
    18601 catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg aaagtttaga
    18661 tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag agttggcctt
    18721 ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa taagctttat
    18781 tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat agaaatggag
    18841 gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag gaaacgtttt
    18901 tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc catggtttac
    18961 ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat gatcactttg
    19021 taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa acaatcagca
    19081 tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt ccgacaattg
    19141 atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct tttttaccgg
    19201 ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg cttaaagccg
    19261 taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa atcgaagaat
    19321 taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata atcaaaaaat
    19381 aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct gggacaaaag
    19441 cgtttaactc caag
//
LOCUS       DACENJ010000030        19392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.030, whole genome shotgun sequence.
ACCESSION   DACENJ010000030 DACENJ010000000
VERSION     DACENJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.030"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(375..1328)
                     /locus_tag="IW748_000801"
     CDS             complement(375..1328)
                     /locus_tag="IW748_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1103725.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1351..2106)
                     /locus_tag="IW748_000802"
     CDS             complement(1351..2106)
                     /locus_tag="IW748_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1103726.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2103..3062)
                     /locus_tag="IW748_000803"
     CDS             complement(2103..3062)
                     /locus_tag="IW748_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1103727.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3055..4005)
                     /locus_tag="IW748_000804"
     CDS             complement(3055..4005)
                     /locus_tag="IW748_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1103728.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4155..4607
                     /locus_tag="IW748_000805"
     CDS             4155..4607
                     /locus_tag="IW748_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1103729.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKSNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="IW748_000806"
     CDS             complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="IW748_000806"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1103730.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="IW748_000807"
     CDS             complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="IW748_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1103731.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8159..8968)
                     /locus_tag="IW748_000808"
     CDS             complement(8159..8968)
                     /locus_tag="IW748_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1103732.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8977..12558)
                     /gene="smc"
                     /locus_tag="IW748_000809"
     CDS             complement(8977..12558)
                     /gene="smc"
                     /locus_tag="IW748_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1103733.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12578..13264)
                     /locus_tag="IW748_000810"
     CDS             complement(12578..13264)
                     /locus_tag="IW748_000810"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1103734.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13612..15393)
                     /locus_tag="IW748_000811"
     CDS             complement(13612..15393)
                     /locus_tag="IW748_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1103735.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15419..16333)
                     /locus_tag="IW748_000812"
     CDS             complement(15419..16333)
                     /locus_tag="IW748_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103736.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16360..17322)
                     /locus_tag="IW748_000813"
     CDS             complement(16360..17322)
                     /locus_tag="IW748_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103737.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17322..18266)
                     /locus_tag="IW748_000814"
     CDS             complement(17322..18266)
                     /locus_tag="IW748_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103738.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18267..19271)
                     /locus_tag="IW748_000815"
     CDS             complement(18267..19271)
                     /locus_tag="IW748_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103739.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5343 a         4193 c         3433 g         6423 t
ORIGIN      
        1 cttattgccg agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa
       61 aatatctaaa tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga
      121 ggcgatcttg atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat
      181 caacccgtat gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa
      241 accatccgta aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat
      301 aggctcttgc tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc
      361 ctctgttatt tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat
      421 tctaaaccgc ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt
      481 ttaccagcat tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct
      541 ccaccaatag cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca
      601 tattcataag aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca
      661 aagccaaata aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc
      721 ccttcattca caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc
      781 tgatcgattg cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag
      841 atctccccta aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa
      901 gaaaggaaaa tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca
      961 gaaatgatga ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt
     1021 ccacctgctg attctacttt tttataggat gaaagatact caggaaggct gcttgtagct
     1081 gctccaacta ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg
     1141 aatacgacga ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt
     1201 atcgtcaatg tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg
     1261 ttgctatttc cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat
     1321 tttttcatct cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc
     1381 aatctcacaa atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat
     1441 ttcttgggtc gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc
     1501 ataacttgca gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg
     1561 tactaaattt tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa
     1621 aggttcatcc agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg
     1681 ttggagttga ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag
     1741 gtgttccagt gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata
     1801 aggaaatcgt ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga
     1861 ttgtcgtagg atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt
     1921 atggtctaaa tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac
     1981 cgttgacttt ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt
     2041 taactgtaca tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc
     2101 aatcatagga tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca
     2161 aattcaacga cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt
     2221 cctcctgtca gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga
     2281 taagtagaaa agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga
     2341 cccacaagag ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca
     2401 aaacgatctg tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct
     2461 agcacatgtc gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc
     2521 aaaagatact gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca
     2581 tattcattcg gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct
     2641 aaaatcatcc ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag
     2701 aaaaataaca atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta
     2761 cctgtctgct gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg
     2821 acatataacg actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc
     2881 tgaaagctga ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc
     2941 ttactcctca atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt
     3001 cctgtaacaa ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc
     3061 atatcgtcgt ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat
     3121 cacactgact ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac
     3181 taagaacaaa ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata
     3241 tattctagag acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat
     3301 caacatgact gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta
     3361 atctattccc agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc
     3421 aaatagccaa accaagagca acaatggaac aacagcatac aataactcat aatttcctcg
     3481 tatcacagtc gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg
     3541 ataagcaaaa aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg
     3601 aagcatcaat tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc
     3661 tgcaaaagca aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa
     3721 aatcatagcc actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga
     3781 aacaaattta ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc
     3841 cacaaggatc aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg
     3901 cccatctaac tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat
     3961 actacttaaa ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct
     4021 aattgagaat gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt
     4081 tcatatgttt taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa
     4141 ggagggtatt ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg
     4201 gtacaccttt agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct
     4261 gctatcttag tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa
     4321 aagaactcaa aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg
     4381 tccaaaacca atatatccag ctagcaacga aaagcaatag ccgtttcact gtagtcaacg
     4441 attacgtcac tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa
     4501 atgaacctgt gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa
     4561 aagaaaaacc aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac
     4621 ataaattttt acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt
     4681 caaaagcttt ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa
     4741 tcttctgcct tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc
     4801 ttcgatgatc ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat
     4861 caaatcgata ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt
     4921 gtaagcttca gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga
     4981 gttctccctt agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg
     5041 ttctttcaac atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg
     5101 attcgttcca cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat
     5161 actgtcacca ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat
     5221 cacaaagaaa cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc
     5281 acgaaaagca atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat
     5341 cgtgatacct aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa
     5401 tctcggataa gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg
     5461 gaacccagct tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac
     5521 gatatagctg tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa
     5581 ttgcagacgc aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat
     5641 ggcatcgaac ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata
     5701 gggacgttca tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg
     5761 actcgatagc tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat
     5821 ttgagtcaat gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat
     5881 ttgcttcatt ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg
     5941 ttgttctagt gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc
     6001 ttctttttct tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc
     6061 tggattgata cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg
     6121 aacacctgtt gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata
     6181 aaattctttc tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata
     6241 ctgtttcata gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact
     6301 gttgcgaata ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc
     6361 actcattggt gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct
     6421 cgctgttttc aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg
     6481 gctgaacaat gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac
     6541 catctgtttt cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa
     6601 attttttaat tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact
     6661 taatggccag atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc
     6721 gagatactga aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt
     6781 ttcgctaaaa tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg
     6841 atcggttttt gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa
     6901 tcgagcttgg tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat
     6961 cattaagtga cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa
     7021 cgaaattgtt tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc
     7081 tatgttaaaa acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt
     7141 agacatgggt tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg
     7201 atcaaacggc tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg
     7261 caattcatta cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa
     7321 aacaagaccg gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg
     7381 accagtggtt gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg
     7441 gattactcgt tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc
     7501 tgcagtatca actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa
     7561 tacgacagct gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc
     7621 gccccagaca actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa
     7681 tactttttta ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc
     7741 tacaccatta acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc
     7801 atttacttca ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt
     7861 ttggacttct gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt
     7921 aattcgtatc gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc
     7981 aaaaaagtct tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa
     8041 tgttttacgc gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt
     8101 tttctcttcg ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct
     8161 actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca
     8221 cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag
     8281 catattggat catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt
     8341 tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca
     8401 taaattccaa aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg
     8461 gtatttcttt gattttagga tctaattctt cttggtcata agcgattact gccttattaa
     8521 gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca
     8581 acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag
     8641 acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg
     8701 atacttccat cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc
     8761 cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg
     8821 ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt
     8881 gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt
     8941 ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt
     9001 cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg
     9061 catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac
     9121 gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc
     9181 ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat
     9241 aatggaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag
     9301 actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc
     9361 agttggttcc gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat
     9421 tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc
     9481 catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt
     9541 tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac
     9601 tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat
     9661 atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg
     9721 cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt
     9781 ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag
     9841 cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt
     9901 taatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac
     9961 ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg
    10021 ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa
    10081 ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc
    10141 tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt
    10201 ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta
    10261 atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc
    10321 aaaacgttca agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa
    10381 acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc
    10441 ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa
    10501 ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg
    10561 tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac
    10621 cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc
    10681 taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac
    10741 ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc
    10801 cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg
    10861 ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg
    10921 ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag
    10981 ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac
    11041 cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg
    11101 tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc
    11161 ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga
    11221 tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac
    11281 tgatgcttct acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat
    11341 atattgacgt tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc
    11401 aacatattga tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa
    11461 ttcttgctgt gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc
    11521 tgctgctttc atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc
    11581 tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa
    11641 aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg
    11701 atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga
    11761 ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg
    11821 tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac
    11881 atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc
    11941 ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt
    12001 gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac
    12061 accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc
    12121 tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa
    12181 ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt
    12241 ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact
    12301 attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc
    12361 aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac
    12421 ccagcggata gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt
    12481 gacgctattt tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc
    12541 aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt
    12601 ccgccgcagc ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat
    12661 catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca
    12721 atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat
    12781 gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga
    12841 atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata
    12901 ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca
    12961 aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga
    13021 tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat
    13081 actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg
    13141 ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca
    13201 aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt
    13261 ccattttcca cattcctttt atggatattt tttcgatact tccttattat actggttttt
    13321 tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg
    13381 acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg
    13441 gctttccata tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt
    13501 ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa
    13561 atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg
    13621 tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca
    13681 gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa
    13741 tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc
    13801 aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg
    13861 agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt
    13921 attccaagca gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa
    13981 agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca
    14041 ttgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa
    14101 gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt
    14161 gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat
    14221 ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa
    14281 acctgcatag aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg
    14341 taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt
    14401 ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt
    14461 atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc
    14521 atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat
    14581 ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc
    14641 tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg
    14701 atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata
    14761 tgaccagata ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc
    14821 aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata
    14881 ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt
    14941 attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga
    15001 ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag
    15061 cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg
    15121 agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc
    15181 tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc
    15241 attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga
    15301 gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat
    15361 cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact
    15421 agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac
    15481 tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta
    15541 agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca
    15601 agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg
    15661 tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa
    15721 aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg
    15781 tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa
    15841 aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg
    15901 gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt
    15961 agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc
    16021 cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt
    16081 catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt
    16141 tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga
    16201 tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa
    16261 attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt
    16321 ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg
    16381 gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa
    16441 aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag
    16501 ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga
    16561 agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg
    16621 actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga
    16681 tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc
    16741 ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc
    16801 accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa
    16861 aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta
    16921 gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg
    16981 cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat
    17041 ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc
    17101 ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc
    17161 tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa
    17221 tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg
    17281 cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct
    17341 ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca
    17401 tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg
    17461 tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag
    17521 atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg
    17581 tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat
    17641 aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga
    17701 acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca
    17761 acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta
    17821 tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct
    17881 tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc
    17941 tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa
    18001 cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg
    18061 gctgttacct tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac
    18121 tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca
    18181 tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg
    18241 tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa
    18301 tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct
    18361 tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga
    18421 ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt
    18481 ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac
    18541 agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca
    18601 gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct
    18661 tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc
    18721 gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca
    18781 cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta
    18841 ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc
    18901 tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat
    18961 gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc
    19021 gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg
    19081 ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat
    19141 tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca
    19201 tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg
    19261 ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa
    19321 ggacattaca cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa
    19381 ttattaataa tt
//
LOCUS       DACENJ010000031        18934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.031, whole genome shotgun sequence.
ACCESSION   DACENJ010000031 DACENJ010000000
VERSION     DACENJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.031"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2808)
                     /locus_tag="IW748_000816"
     CDS             complement(<1..2808)
                     /locus_tag="IW748_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103740.1"
                     /translation="MKKLKLISIALFSLITMSTARQSIVSAEGTSTKAVVEESGDKKV
                     AIDDKVKNEAEKATVVQGADGLMYVVVDENDPEKLLEAESPLKNQQENTGAVEVGVDE
                     KRQEKADKANEKVKNFDDIDYNDISSLQSALNDPKFPFLSEDELNEKLQKTKNTFESE
                     ALMKKTRGYFSQRILELSNNLNDVPKRAKVSRHALVSNRASQVSETRINPPNGYPWVI
                     YLIRSNGYSYTENYAIRRTIDGKEAFCLQAGVQATNTYTGSTSGYLSNSQREMISSIL
                     SAYKELGGWANNDAGKQRWIATQMLVWENSNEKPKSITVTPNWKNGATKWKNDLVARA
                     NAIRVRTKWDSTSKTVDEGDKATFKVTNTYGDHNLHVKSVSGGGKATISNGTLTVDTT
                     GATGNKITVDMIKGSHPSDAHPTTVWSNGSYQKLVTGSFYISTSVTVNVKRKGTVEVL
                     KVDSETKKPLSGATFRFSYSGKTKDVTTDSNGKAKLTERLKAGTVVKVKEIKAPNGYQ
                     LDSSEFSITVKENQNVTTTRTNKKSTGSVEIEKIGDLDGLGLPNVVFTIYNSDNKVVK
                     DNLKTDSNGKIKVDELQFGKYYAVEKQGVTGYDPDGKKYNFEISQDTPFTKPAKVKVT
                     NIYEVSISEKVSDMNESQVLKNNWSADPNDYLQYDITTGNLKKSGERNVQSFIIEGFY
                     DNKNVDVSKSEVYVGSTNVTNSFDINNDTNNGKVIAKAKTSVLTNNDFYNKSYNLRIT
                     MKIKKSTLENTEKQKNLTIVKNSKVTVGSKISKSASSNDVETVLYSRKVTINHIDEKD
                     KHLLKQDIDYKYDGETYEYKPRTDLFDKDGNNYKSDVIHKGKIDGKDIVLNTPYHIPV
                     LSVNVDRIQIDTARAVKNGYLPTKIELSKKEEYEKELEKIVFKVKITDIDNNKVVYDE
                     NFKYKEFNSHMSLDLPTEYLTK"
     gene            complement(3226..3549)
                     /locus_tag="IW748_000817"
     CDS             complement(3226..3549)
                     /locus_tag="IW748_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103741.1"
                     /translation="MARSRIAKIEQTKKDKKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKNKG
                     IMIPA"
     gene            complement(3694..4137)
                     /locus_tag="IW748_000818"
     CDS             complement(3694..4137)
                     /locus_tag="IW748_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1103742.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLSHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSVVFYT"
     gene            complement(4457..4894)
                     /locus_tag="IW748_000819"
     CDS             complement(4457..4894)
                     /locus_tag="IW748_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103743.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            complement(4907..5761)
                     /locus_tag="IW748_000820"
     CDS             complement(4907..5761)
                     /locus_tag="IW748_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1103744.1"
                     /translation="MMKSNFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            complement(5841..6077)
                     /locus_tag="IW748_000821"
     CDS             complement(5841..6077)
                     /locus_tag="IW748_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103745.1"
                     /translation="MLSDYKEELKLIENRVLGFVECKDNLDDTLEVLESIIFNTKVLD
                     LKAINGDMTTHIILDEISAKEIGETLIAWANKEL"
     gene            complement(6429..6653)
                     /locus_tag="IW748_000822"
     CDS             complement(6429..6653)
                     /locus_tag="IW748_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103746.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            complement(6765..7364)
                     /locus_tag="IW748_000823"
     CDS             complement(6765..7364)
                     /locus_tag="IW748_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103747.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            complement(7682..10045)
                     /locus_tag="IW748_000824"
     CDS             complement(7682..10045)
                     /locus_tag="IW748_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1103748.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGSYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
     gene            complement(10094..10471)
                     /locus_tag="IW748_000825"
     CDS             complement(10094..10471)
                     /locus_tag="IW748_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ1103749.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLIC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            complement(10605..10904)
                     /locus_tag="IW748_000826"
     CDS             complement(10605..10904)
                     /locus_tag="IW748_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1103750.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            complement(11138..12739)
                     /locus_tag="IW748_000827"
     CDS             complement(11138..12739)
                     /locus_tag="IW748_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1103751.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            complement(13136..13390)
                     /locus_tag="IW748_000828"
     CDS             complement(13136..13390)
                     /locus_tag="IW748_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103752.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            13956..14252
                     /locus_tag="IW748_000829"
     CDS             13956..14252
                     /locus_tag="IW748_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103753.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            14259..16211
                     /locus_tag="IW748_000830"
     CDS             14259..16211
                     /locus_tag="IW748_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1103754.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(16283..16780)
                     /gene="dfrG"
                     /locus_tag="IW748_000831"
     CDS             complement(16283..16780)
                     /gene="dfrG"
                     /locus_tag="IW748_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1103755.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(17174..18058)
                     /locus_tag="IW748_000832"
     CDS             complement(17174..18058)
                     /locus_tag="IW748_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAQ1103756.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            complement(18045..18608)
                     /locus_tag="IW748_000833"
     CDS             complement(18045..18608)
                     /locus_tag="IW748_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103757.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGEFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
BASE COUNT         6022 a         3422 c         2445 g         7045 t
ORIGIN      
        1 ctttgttaaa tattctgtag gtaaatcaag cgacatatga ctattaaatt ctttgtattt
       61 aaagttttca tcataaacaa ctttattgtt atctatatct gtaattttaa ctttgaatac
      121 aattttttct aattcttttt catattcttc ttttttactt agttctattt ttgtaggtaa
      181 atagccattt ttaacagctc tagccgtatc aatttgaatc cgatccacat tcacagaaag
      241 taccggaata tgatagggag tgtttaaaac tatatctttt ccatcaattt tccctttatg
      301 gattacatca gatttataat tgtttccatc tttatcaaat aaatcggttc taggtttata
      361 ttcatacgtt tccccatcgt atttataatc aatatcttgt ttcaaaaggt gtttgtcttt
      421 ttcatcaata tgattgatag taacttttct tgaatataaa acggtttcta catcattaga
      481 gctagcagat ttagagattt ttgaaccgac agtaacttta ctatttttaa caatcgttaa
      541 atttttttgc ttttcagtgt tttcaagagt agatttttta attttcatag tgatccgtaa
      601 attataggac ttattataaa aatcattatt cgttaatacg cttgtctttg ccttagcaat
      661 tacttttcca ttgttagtat cgttattaat gtcaaaagag tttgttacat ttgttgatcc
      721 aacatatact tcactttttg acacatctac attcttattg tcgtaaaacc cttcgataat
      781 gaaactttgg acgtttcttt ctcccgattt ttttagattt ccagttgtaa tgtcatattg
      841 aagataatcg tttggatcag cagaccaatt attttttaat acctgacttt cattcatgtc
      901 cgatactttt tcgcttatgg atacttcata aatatttgtt actttcactt tagccggttt
      961 tgtaaaagga gtatcttgac ttatttcaaa attgtatttt ttaccatcgg gatcataacc
     1021 agtaacacct tgtttttcta ctgcataata tttaccaaat tgcagttcat cgacttttat
     1081 tttcccatta ctatctgttt ttaagttatc ttttacaact ttgttatcgg aattataaat
     1141 cgtgaatact acattcggta gtcctaatcc atctaaatcg cctatctttt caatttctac
     1201 agaacctgtg gatttcttat tggttcttgt ggtagtaaca ttttgatttt ctttaactgt
     1261 aattgaaaat tcgcttgaat ctagttggta gccattagga gcttttatct ccttaacttt
     1321 tactacagtt ccagctttca acctttcagt taattttgct ttaccattcg aatcagtagt
     1381 aacatctttt gttttacctg agtagctaaa tctaaatgtt gctcctgata atggtttttt
     1441 tgtttctgaa tcaactttta aaacttcaac agttcctttt ctcttaacat ttaccgtaac
     1501 agaagtacta atgtaaaaag aaccggtaac aagcttttga taagaaccat tactccatac
     1561 cgttgtaggg tgcgcgtctg aagggtgaga acctttaatc atatctacag tgattttgtt
     1621 tccagtggct cctgtagtat caacagttaa agtaccattg cttatggtag ctttgccgcc
     1681 accagataca gatttcacat gcaaattatg atctccataa gtattagtta ctttaaaggt
     1741 tgctttatct ccctcatcaa ccgtcttaga cgtagaatcc cattttgttc ttactctaat
     1801 agcatttgca cgagccacta aatcattttt ccacttagta gctccgtttt tccaatttgg
     1861 cgtaacagtt atacttttag gcttttcatt actgttttcc caaacaagca tttgagttgc
     1921 tatccatctt tgttttccgg catcattatt agcccaaccg cctaattctt tatatgctga
     1981 taaaatcgaa ctaatcattt ctctttgcga attagaaaga taaccacttg ttgaaccagt
     2041 gtatgtattc gtcgcttgta caccggcctg taaacaaaaa gcttctttcc catcaatagt
     2101 tcttctaata gcataatttt ctgtataact ataaccattg ctcctaatta aataaatcac
     2161 ccacggataa ccatttggag ggtttattcg tgtttcactt acttgtgaag cacggttact
     2221 aactaaagca tgccgactca ctttcgctct tttcggtacg tcatttaaat tattgcttaa
     2281 ctctaaaatc ctttgactga aatatcccct tgtctttttc attagcgctt cagactcaaa
     2341 tgtattcttt gtcttttgca atttttcatt taattcatct tccgataaaa aagggaattt
     2401 cggatcattc aacgctgact gcaacgaact aatatcgttg taatcaatat cgtcaaagtt
     2461 tttcactttt tcgttagctt tatcagcctt ttcttgtctc ttttcatcaa caccgacttc
     2521 aacagcgccg gtattttctt gttgattttt caaaggagat tcagcttcta gcaatttttc
     2581 aggatcattt tcatcaacga ccacatacat caagccgtct gccccttgaa ccacagtcgc
     2641 tttttccgct tcgtttttca ctttgtcgtc tatagcaact tttttatcgc cagactcttc
     2701 tactactgct ttcgtagacg ttccttcagc cgaaacaatt gattggcgag ccgttgacat
     2761 agtgattagg ctgaatagag caatgctaat cagttttagt tttttcatta tcacacttcc
     2821 tttagaaaga aatgtaataa agaatcataa caactcttta ctacatttct tgtagtaaag
     2881 agttaccctg tatctagcac atttattact gtgctgaatt ttttagtgtc aatattctga
     2941 tctctaggac ggttttcata ggttcttcct agatagaaat tctgaataat taccatttac
     3001 gccacctcat aattagtagt ttcaatggat cgacttttgt attttttggc gtgaaatact
     3061 aaccttatca cttctattgc taacgtattt acatacctct tggcaaagga aataacagta
     3121 tgcttggttg ctattttgct tattagcctt acgcttttat ctgtaacata gcaacattgc
     3181 tacagtacga gtagtattaa gttttcaaag aacaatttca ctgatttaag ccggaatcat
     3241 gatcccttta tttttacagt aagcaagacc agagtttctt gatttccgtt tagccacgat
     3301 taaaccttta ctatccacaa tatttcttat ttccggataa agttcatcaa tcgttttaaa
     3361 attactgaag ttaccaagct gtacaaatag accattcatt gaaactttaa cataacgatc
     3421 ttcatcaaat gttttcacag accatgctat cctcttaaca tcttcagctt taaagaattt
     3481 tgttttattt ttagaaattt ttttatcctt tttagtctgt tcaattttag caattcttga
     3541 tcttgccaac agctatcact cctttaattt ttttactaaa tcagttcata cttaattata
     3601 tcatttatga caatttctat caacctgata gataaaaaca aaataaaaaa aatagataaa
     3661 aaccttgtat ttttaggttt tcgtctattt ttttcaagta taaaaaacta cacttaacag
     3721 tattacaatt aaattgtaaa aacaatgaat agtaaccgga acaatcagcc ttttatctga
     3781 attatcatag acaaaagaca aaattaaact cgtcataaaa taaggaataa aatataaatt
     3841 taagtgggat agactaaaca aaattgcaac aattatatat atgcttcttg tagacaaatt
     3901 tgtttttaat tccaattggt tttggataat tcctctcatt aaggtttctt caaagaaagg
     3961 agcaaaaatc ccacttatga aaaacgaaat aaacgttact aattcagtta cattgtcttt
     4021 tctttgatta acaaagacca acgctatcat cgaaaggaat aaatttacta caagaagaac
     4081 taatgtatag cctattatcc ttttccaata aattttaata tcttctaaaa tctgcataat
     4141 tagttaatct gcataatgat ataaatagct gtcgctatta aaattaggta cgaccacttt
     4201 ccaagcaaag aatatacagg cgaccagaaa aaagagagta ctttaaacat aaaccagatt
     4261 atcaatatat agccaaaaaa actccaaaaa ttagggacaa accaattgat aaatactaaa
     4321 gcattaggaa ttatagccca tttaaccaat gcaataattc cacctaccag tagaaaaacg
     4381 gctactacat acttccaaat ataattttct ttgtatgaat aatttgattt atgatccata
     4441 gtcttacctc atttcattat gcttttcctt ttcttttatc aagataccat gtcaaagctg
     4501 aagcaataat attagtatta gattgatctg tttcaaattt tataactttc aaaactttaa
     4561 aggcttcttc agaaattttt actggtctcg ttttcaactt gccaaaatca gtaactatac
     4621 tatttccatg tgtttcaaat tcttttaaaa ataaagctaa aatttctgtc attaatgaat
     4681 atttagcccc gtatgataaa tctttaacaa cttcatcgta taattcaact ggtaccctaa
     4741 cagatttccg tttatcacca gtagacttaa tgccagcttt accttttaac atttgttgtc
     4801 ctatattaaa tccaccacta ccgcttttta ttccgaacgg attacccgaa ccattactgc
     4861 cggcactagt tactttttta gttttcgaca tcatgcaaca cctttcctaa acacgttcta
     4921 aaatattttc tcctgtagct ttttctaaac gtactaacgt ttcttctaca atgtctacat
     4981 acatgctata agcagtttta gaatgtggga tagatttatt tagtccttct ctaccccaac
     5041 gtttaattac gcctcgttct gaaacaatat gagaataaac taagtctcca tattcttctt
     5101 gaagttctga taaaagttct aatcctgttt ttaatttagt atccactaga tatggaacaa
     5161 tacctaaaat ttctaaatct ccgtttactt cttttacagc agtttgataa ttagtaaccg
     5221 tactatgcaa gttgtctata gaatcttgct cagtttgcgt gggaattact aaaaaatcac
     5281 tcgccacgta aacgttatct gtaaattctg aaactgtagg aatagtgtca aacaaaataa
     5341 aatcatattg atctcttaaa ttatcattac cgatcatata attaaacaaa ttccaaaaga
     5401 cttcttcacc ttcatcagta aaaccagtac ttctccacat atctcttaat actttggtaa
     5461 accttgacat ttgttctgaa ccaagcacaa tatctaagtt ttctttaaca ggagaaatgc
     5521 agtctaagaa agacatatta attactgctt caaacaatgt cattttccca acaactttta
     5581 aatcatattg tctttgaaac tttttagtta ctgttccttg tggatcaaca tcaataacta
     5641 aaactttaaa gcctctagcc gatagaatat cagccattag ctgagtaaga gttgacttac
     5701 caactccccc tttaaacatg ttgattgcaa ttacatatcc caaaaaattt gatttcatca
     5761 tgtatacctc acttttgata atttattata ctatacagac tacttgtagt caattggcca
     5821 ctttatttta aaattatctt ttacagctct ttattagccc atgctattaa agtttcgcca
     5881 atctcttttg cgctaatttc atctaatatg atatgtgttg tcatatcacc attaattgct
     5941 ttcaaatcca gaactttggt gttaaatata atactttcaa ggacttctaa agtatcgtct
     6001 aaattatctt tacactctac gaaccccagt actctattct ctatcaactt tagctcctcc
     6061 ttataatcag ataacaaaaa aattctctaa aagtcctaca aaaaaatcta gtaaaaaagg
     6121 tcaaaagtga acgattcatt aatataattc gttattttcc attatgagcg gttttgtaat
     6181 aatttcaata tttttttcta cgcttgaaca atacatagat attaagaaaa tatttagtga
     6241 aatttatcaa cgctcattta ataaatcgtt cctcaaaata ttcatattcc tcctttcttt
     6301 aggccataat cgtctgtttt tattcccgat ccctctatcg actacttgta gtcattagac
     6361 ttttagaaaa gggatatttc tatccctaaa accattctac accattgact acttgtagtc
     6421 aatggttatt attcatcatt ttgaccgtta tttttccgat cttctaacca tttttccaac
     6481 aattcaatat cttcatcttc agacatttca atgaattttt tagcaaatga tttggctctt
     6541 atataggcaa tagtttcctg atttttttca acccattttt tcctagcctt tttcattgct
     6601 tcttttcttt cttcttctgt attgtatttt ctcggtctcg ccatagaaat catcctttaa
     6661 gttttatctt ctacagtata ataacattta aatacatatt cgattaaaat tcaatgtatc
     6721 ataatatcca atagaataat aaaataagat aggtttagcc tatcttactc tacatctata
     6781 ctttgagttt gtacatgaat atttctttgg gattctgcta tactaacagc ttccaatgat
     6841 tctacattcg tcattatttt ttgataagta attacttctt tacgattttt cttaaagtaa
     6901 ctaggatcaa gtttacctaa cttagaatta taaagtaatt cgcatgattc atttatttca
     6961 gactcatgta atttcaaata ttttgcctgt ttgattacta acatcttata cttctcatca
     7021 acatttgggc tattaggtgt ccatactttt gcacaatctt ttggcacagg aatcatatta
     7081 tttatatgaa gtactcctaa aaagtccttt tcttctcgat caccataaat cttaaacact
     7141 gatttattag tcaaagaacg ttgattttct tttgctgaag ccaatggtac ataataattt
     7201 atttcttcat ttttgttaga aattttcaag tttgatttaa aatataatcg ttgttctcga
     7261 tagttgtttt ggacacgata gtccaccttt ttcagcttat ttatataatc atctgaaatt
     7321 acatatagtg tagatttgtt tgagtttgta ttctttagat tcattggatc tccccctttc
     7381 caatggatta tatatgctaa gattaacagc ctataatctt ttttaaaact aattgctctc
     7441 attttacatt taattcataa aaatatattc acgtttatgt aattttattg gtaaaacaca
     7501 ctatacatat caaaacggta ttattctact tccaatttaa caaaatagca atctaatttg
     7561 tttgtatttt ttcaactgtc ctctaactag ttttgtattt atccttaatt cagtatacct
     7621 atattttggg cacttttgaa taaaaataag cggaaaaaca aatgatttcc gccactaaga
     7681 attaaaattc tttctttata actttgtttt tattatcaaa gacaagataa gcactatatt
     7741 ttttattgtc tttacttata aaacccgtta ctttctcttt agttttccca ttttctaaca
     7801 attctttgac tgtttttgaa cttaaagttt tcccactatt atttttatag aaaataaaac
     7861 tttcacctgt tgctaattct tttatttcaa atacttttgc tttttcaact atttcataac
     7921 catttactgt atcgtgtttg ctctttttat cgccttttgg actgttcttt tcaaactcca
     7981 ttaccacttt catcacattt ggatcgaaaa gtaatcttgc attaaaagct tttccttctt
     8041 ttgacttaaa gccggaaagg actttatctg tacgattatt tagtaatagt tcttgaagaa
     8101 cttttttatt gattgctttt cctgatatat ttttaaatat cacgaactgt tcaccactag
     8161 taatatttaa aatagaataa acttttttct tttcttccac ttgataacta cctatatcaa
     8221 atttattttg atttacagtc ttttctaaat aatccgaagt aaattctact tttttcaaat
     8281 ctgaaacagt cttattgata tagttataga tatttcttaa aaaagcttcc tgtgtatagc
     8341 taccttgcgc aattttccca agtgattctt cccatatcgc tgtattttct ggttttccaa
     8401 tcaataggtc tttattcttt gtcaaatccc ataataaata tccctttgga gtaggataaa
     8461 cattcgtgtt ttctatctta atatattccc tatttagaag agtgtctaca atattagaac
     8521 gtgtagcggg cgttcctagc cctaatttcg ccataatacc ggttttacct aataatttac
     8581 cttcagtcaa ccttttaggt tccttagtta atttatcaat aaatctaata tctatattta
     8641 ttgtctcttg ttcatgaaat ttaggtaatg tttcctcttt atcatcagat tcatctaaat
     8701 tagtatctac ttgcttccaa cctaaagata taggtacatt accacttgtg gtaaattcta
     8761 agccgtttac atctaatgta acttttgtat tttcgtactt gtaaggttct gcaaacatta
     8821 gacacgcacg cctagtaacg atctcataaa tatttttttc atctaatgtc agatttgcta
     8881 tatcaggaat atccattgtt ggtattagtg cataatgctc taatactttg tcattatcta
     8941 caaaccgctt tttaggctct aaacaaggtg tatcaatact gatccctaaa acactcttat
     9001 acacgtctaa attcgctttt aaatactcaa attcatttgt agttatcaat tcactatcgg
     9061 ttcttggata agataaatat ccacgatcat atagactttg gataatttta tctgtttttt
     9121 tagcagaata tttccatttt ttattagcgt attcttgaat accacccaag ctaaacaatt
     9181 tttgtggttc tgtttgcttt ttactaacct caattttttt tatgattcca gtactggcgg
     9241 aatcaaggcc gaatttttta gtgtcttctt caaatttttc taatttaaag tattcaagac
     9301 tattcttaaa tattgctttt ggttgcttat cggttgtatg acattcaatc gttttatatt
     9361 tttccggtct aaaatttcta attgccaaat cgttttcaac cacaagtgtg tttaccggtg
     9421 tctgaactcg accgagtgat accacacccg atataccttt ttcttgcaac tttaatgaat
     9481 acaactctgt ataattcatc cctactagat agtcagcaat ttgtctagca ttagcttctt
     9541 tgaagtaaga gatcgtttct tcagaatttc gcagattttt aaatcctttg ataatttctt
     9601 tttgaaccaa agaattaatc cataatcttt ttatcggttt attaaaaatc gactgcttgc
     9661 ataatttgaa aatattataa gcaatatttt ctccttctct atccggatca gtagcaatta
     9721 ttatctcatc tgcattttct aaacatgact tcactattga aaattgttgg ttttttcctt
     9781 tagagacttc atactttaat tcattgaatt taggtagaaa aggcaaatta tctaaatgcc
     9841 atttatcttt attcggtagc cactcatact tttcaggtaa tgataatcct accaaatgac
     9901 ctattcccca agtaacaata gtatctccaa atatttcact tttaaattca tacttcccac
     9961 tctttttcga taattttttt ccggccaatg ctgaagcaaa ttttgctcct tgatctggtt
    10021 tttctgctaa tataactact gccatatact tcctccactt tcttcaattg atttttaacc
    10081 aaaagttctt actctatgac tgactacttg tagtcgaaag tagtatatct ctaattcttg
    10141 taaatttagc attacttgtc cgttcacaaa ttaaagttat ttttgatcga tcaggtaaaa
    10201 atcgtacact ttctttagcc acatctactt cccttttagg tacaggttca caacaatatt
    10261 ttgtattact ccaaaacgaa aattttaact ttcttccctc aatcgtatca acaagtaaaa
    10321 ttatatttcc tttcttcccc caatacctac agacagtttc acctacaatt ttaaataaac
    10381 ctatttcatc aggaaataaa tactcttttc tatcaatatc tactatgcct tttttaaaat
    10441 ctgattcatt ataaatccca tcaatattca ttaatagccc ccttttttat ttacctgata
    10501 tatattaatt ctaacaaaaa aacagccaat atcaaactat caaaaaacag agtagctatc
    10561 tgtctacttg tagtcaaaaa acaaattatt cttttcttct ttctctaatc ttcaatatat
    10621 tttttattcc gttttggggc tgaaattttc aaatactctt tatactcttt ttcatactca
    10681 cacaagcaat cataaacctc attcatattc cctcctgaag ctagagacaa agcatacaaa
    10741 aaagaaatag gcaaatcagc tacctcacgc tctctagtaa tccaagacga tagagttcct
    10801 tgtttaaatc catataactc acaaaacacc tcaacagata aacctagctt accgataata
    10861 aatacattga tcgggtgtga gtaaacatat ttcttaatta ccaaagaaaa tcttcctcct
    10921 tcaaacataa ctgcatacgc agttataagc tgtattccgt acacgtggat taagggaaaa
    10981 gacacaagta aaaatagttc tatacactcc atacataaaa gagagtacta actactaatc
    11041 attagtatac gctaaaacag gaataggagt gggcagaaac actagatatg ttaattggca
    11101 atttagctat ttaaaaatca aaaaaataaa tagaactcta acctgtatta gctcctctaa
    11161 ttggcgcatg tttgctcttt tctctttctt tcagtatgtt cgcatggaac ggtactgttt
    11221 tgtcttcata acgtcttaaa atcgtttcta cgtggctatt tgctgttcct aataattcta
    11281 agacaaatac ttgtttatct tttttattct gaataactgt atttaatatt ttagcttcca
    11341 caaatttgct tgttgctatt ttagtcgatc cataccgacc acgagacgtt cgtaatatca
    11401 ctgtattcgc cgttctaagc tcttttaaaa tcacctttaa cgtagataca ggaatttgtg
    11461 tttcttcaga tatagttccc atagagacac ttaaaaacaa ctcatttttt ttcaatgatc
    11521 tttgttttct ttctaagtaa gctaataaat cagctttact ttcttcaaaa tgactatatt
    11581 ttcgttcttc tctaggttta gcaaatttca cccaattaga cggatcgaca cgattccctt
    11641 tacgtttaac agcttgtcta tttacattta ttgagtattt ttcaaactcg gtaggtgtag
    11701 cataattttc gataatttca ttgacatatg aacttttagc acctttgtaa tcgccacgat
    11761 aagcacttct tattgttctc tcaacttcag aataggacaa atcattattg aattgaagtt
    11821 gatccaattg atccatacac tcgccaatag ataagccact tgatttacat gccagggcta
    11881 aggtaaaaat agttttattt ctatctcctt cagcaatatc cgtatgaatt aaaaagtcgt
    11941 accatttagt atcaatttgt cgtttagacc ctttcttgcc agtagaatta ggagctaata
    12001 atgacaagtt agacctcttt aaagcatatt ctttttcttc aaagtctttt gaccatttca
    12061 aatacccttc aaacgtatct acgaaattag gttcataatg tacaatattt tttttagttg
    12121 gaaatctaaa aataccaaaa tgattacagc ccatatcgac ttgtggtaaa cgattttgga
    12181 tagattttct gatattttct gatattcttt tagcaactct caaagattta aaatcatttg
    12241 aattagaaat atagcttgcc ttatcaaatg aatcagtttc ttggttatag ttttgaatca
    12301 gaaaatagca atgataacct tttggcgtat ctacaatcag tgtaggcaat aacccttctt
    12361 ccaatagata aagaactagt aaattcaaat ccgttttctt ttctcctact ggatagtcaa
    12421 tatctgctac aaaagtatta atctgaatta agttagcttc tttgtgacca aaaattgttt
    12481 tgttttcacc gccaccaagc catgaataca cgttaggagt ccagtgtgtt aatacatctg
    12541 aatcttccaa gacacgatca atagaacgat atataagacc tttagtcttt atcatgtttg
    12601 ttttagaagt aaatccggca atagcaccaa tcttattgct attcttttta aaagcaacta
    12661 gatttttgct attaaactgt ttaattccat ttttaagaat tttagctaaa acgtccgcat
    12721 actcgcaact attcaacatt gcactttctc cttgctatca attttgaata gcaaaaaaga
    12781 catagggtaa ctttagattc cgtaataagg agtcctaaaa ttacactatg tctttagagt
    12841 aagcacttaa atgctaaact ttaaacgtgc aaaaattaaa atgagttggt agctcatctt
    12901 ggtggacttc gtggattgcc aaatcgcttt tcttactaca gaagttccat taaataagat
    12961 tggtagtctt atttaattat ctttttcgct atttttttcg ttacattcat acatagtaga
    13021 gagtctgtaa ctaacttttc tactagaatt ttttagtgtt cctttggctt cagaactaat
    13081 accaacatta taagctaata gaattacttt taacaagagt ttttttaaaa gatttttacc
    13141 ccatcacttt tacttgattt ataacataag tatttttatc aataatttca tgaatgcaat
    13201 atctgatttt taaagtattt atttttttca gtgttgtttc gtctttggga aaaggcagtt
    13261 tccaaattag aatacatcct gattcagtat taaaaattat ttcagtttct gaaatatagt
    13321 acttactttt aacaagttta acattaccta tttttacgat ctcatctatt tgatctccta
    13381 cgttatacat ttttgtttct cctttcttga tatatatatt atatcacctt gactacttgt
    13441 agtcaacttg gaataaaaga aatagtctac ttgtagtcat gttttcaatt ggaaaaaaat
    13501 ttatttaaag attcttttaa ggaaacgctt aaacaatatt tcttagcttt tttacttgca
    13561 aattcaggaa agtgactgta taatcattta tataaattat gaaataaaga cttcgtggat
    13621 tgccaaagat ttttcttact acgaacagtc ttacgagtca acgattgttg gtagctttcg
    13681 ttggctcttt atttttgcca caaaagacta cttgtagtct tttgaaattt cattttcagg
    13741 ctacttgtag tcaaaaaaaa aggaaagaca ttttcattta agtagttcag ttttggagta
    13801 caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt
    13861 ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta
    13921 tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt
    13981 tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata
    14041 agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat
    14101 acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt
    14161 tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg
    14221 aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag
    14281 ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag
    14341 atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa
    14401 tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt
    14461 actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa
    14521 aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta
    14581 tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt
    14641 tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag
    14701 atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat
    14761 tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc
    14821 aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt
    14881 atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt
    14941 cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg
    15001 agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa
    15061 caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg
    15121 tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa
    15181 cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct
    15241 atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc
    15301 atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac
    15361 catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta
    15421 ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact
    15481 attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact
    15541 tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac
    15601 atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg
    15661 gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc
    15721 tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag
    15781 aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc
    15841 cattagccac taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc
    15901 tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct
    15961 ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg
    16021 gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta
    16081 caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt
    16141 tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt
    16201 atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt
    16261 tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat
    16321 agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt
    16381 cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga
    16441 tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa
    16501 aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac
    16561 aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg
    16621 ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat
    16681 ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca
    16741 ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa
    16801 ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt
    16861 tagttgaata agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat
    16921 tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat
    16981 gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa
    17041 aactgaacta cttaattttc attgtctttc ctttttttta cgatctttcg ttagtcttct
    17101 gtacggccca atcgttcttg tactttcttt tgaaggcgtt cttctttcag ataatgtttt
    17161 tctgtacact atgttagtga acttcagcaa attcattttc tgccccctgt tcactaagct
    17221 gttttggatt ccaaactagt tcataatctc caaagaaatc tttcaaaacc ataggttcat
    17281 tattaaatat tgctcctgtg tcgtacaggc tctcctgaag agttttgtta taactaccag
    17341 ttaaaaatac ttctttactt ctatagatat atttatccca cttactatcc attttaaata
    17401 aaaacagctc aacatttttt aaatttccat aaggtgtttt taaacaatag ctttcctttt
    17461 gaatatcaga aaagacttgc tttacaatat gtttaaacag cattttttca gctatattac
    17521 taactaaata taggagtaaa aatgcaccaa gtttagttgt cgtattataa taattttgaa
    17581 aatgaatttc atgctcatac aggctaagta gaaataaaac tgtgccctgt ctacaaatga
    17641 tttttattcc tttttgaaaa ggcttcaaat aattaatata attgtcattt gttgacatca
    17701 ttatttttcc tacttgtaag ataaataaag caaaaataaa caggctgaaa gcaagacttc
    17761 ctaaaatatt taaggcttct ttcacctcta atgattcttt taatcgatca gttccggcga
    17821 acagtttata aaagctataa gcattgctcc atcctactct agcaatgaat agccagacca
    17881 gataacctaa acaactaaaa ctaattttca gccctctcac tatatctaat tgaaaaatga
    17941 gtctaaattc cggctctcta tacatacttt taaatttaac aatttctttt ttcaatccga
    18001 gcgataatga ctctttgcct atttttttac tcattttact ccccttaaac cgcttcatca
    18061 gcgatttctt tctcataaag tgaataaaaa acagacaatt taattggctc taaatattct
    18121 aaagttgctt cagggattct tttactgtct ttaaccgcaa tataagctga atcattaaaa
    18181 taaaaaaatt tttctacatt actttttgca ctaaaaccat cataaaaaat tccaaaaagt
    18241 tctttactac gtttttttga aaatggattc accaattcaa aaacacgctt tgaaattttt
    18301 tctagcaatt ctttatttgt tctatagaat ttactatttt tattgattcc ttttaaatct
    18361 tctcgaccac taatactatt tttgaatgta gttactttat ttctactata aaattctcca
    18421 tatattgata cttctaaagt accatcttgc cataccacca aatgatctat atctatagga
    18481 tcacttaatt tactgattaa atcagcaaca ccattttcta aaatgtaatc taaattatta
    18541 tttaatactc tcgacttaac gtactctgat acgttagaat tttttagtgc tttataaata
    18601 gctgtcattc tttattctcc ttcgtagcct tttgatctac gaatttaact accctttttc
    18661 tatatacaac cctcctcatt caaaattaga tagtcaagac gcttaacaag actacatttc
    18721 tattttgtat gatttaccgc tataattcaa cctcaattaa aatttatttc gtgcctaacg
    18781 gatttaaata ttcagttact ttctttatta aaaacagcaa actataaatg ataataaaat
    18841 caaacactat taaaatcata cttgatatta aagctttatt tgttaatgtt gtaatcccaa
    18901 tagtaattac tattaagttt gtgattataa ttct
//
LOCUS       DACENJ010000032        18759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.032, whole genome shotgun sequence.
ACCESSION   DACENJ010000032 DACENJ010000000
VERSION     DACENJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.032"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(170..1426)
                     /locus_tag="IW748_000834"
     CDS             complement(170..1426)
                     /locus_tag="IW748_000834"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1103758.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            2006..3307
                     /locus_tag="IW748_000835"
     CDS             2006..3307
                     /locus_tag="IW748_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1103759.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(3376..3975)
                     /locus_tag="IW748_000836"
     CDS             complement(3376..3975)
                     /locus_tag="IW748_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1103760.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            4229..5110
                     /locus_tag="IW748_000837"
     CDS             4229..5110
                     /locus_tag="IW748_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103761.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            5123..6733
                     /locus_tag="IW748_000838"
     CDS             5123..6733
                     /locus_tag="IW748_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ1103762.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(6810..7469)
                     /locus_tag="IW748_000839"
     CDS             complement(6810..7469)
                     /locus_tag="IW748_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1103763.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(7752..8417)
                     /locus_tag="IW748_000840"
     CDS             complement(7752..8417)
                     /locus_tag="IW748_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103764.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(8564..9169)
                     /gene="dhaL"
                     /locus_tag="IW748_000841"
     CDS             complement(8564..9169)
                     /gene="dhaL"
                     /locus_tag="IW748_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1103765.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(9197..10180)
                     /gene="dhaK"
                     /locus_tag="IW748_000842"
     CDS             complement(9197..10180)
                     /gene="dhaK"
                     /locus_tag="IW748_000842"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1103766.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(10182..10577)
                     /gene="dhaM"
                     /locus_tag="IW748_000843"
     CDS             complement(10182..10577)
                     /gene="dhaM"
                     /locus_tag="IW748_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1103767.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            10731..11051
                     /locus_tag="IW748_000844"
     CDS             10731..11051
                     /locus_tag="IW748_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1103768.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(11163..12032)
                     /locus_tag="IW748_000845"
     CDS             complement(11163..12032)
                     /locus_tag="IW748_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1103769.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(12128..13432)
                     /locus_tag="IW748_000846"
     CDS             complement(12128..13432)
                     /locus_tag="IW748_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1103770.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(13429..13938)
                     /locus_tag="IW748_000847"
     CDS             complement(13429..13938)
                     /locus_tag="IW748_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103771.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(14113..16248)
                     /locus_tag="IW748_000848"
     CDS             complement(14113..16248)
                     /locus_tag="IW748_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1103772.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(16331..17986)
                     /locus_tag="IW748_000849"
     CDS             complement(16331..17986)
                     /locus_tag="IW748_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1103773.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(17988..18302)
                     /locus_tag="IW748_000850"
     CDS             complement(17988..18302)
                     /locus_tag="IW748_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1103774.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            18472..18732
                     /locus_tag="IW748_000851"
     CDS             18472..18732
                     /locus_tag="IW748_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1103775.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
BASE COUNT         5243 a         3847 c         3363 g         6306 t
ORIGIN      
        1 cgaattgttc acacttttga aataattaaa aaaaacgagg gaatttacaa atgtaaactc
       61 cctcgcaaag attgtattaa cgacaaccag cgttctattt taggatgggt ttcaaaattg
      121 gaatgttgta atagtatttc cctttttcaa ggtaaaaact tactaaattc taatcaatta
      181 ctttggccaa aaagtaattt ttcttacctt ttcgaataac cacaaactta ccatcgaatt
      241 catcggatgg atttatgaca aaatttaaat cggtaacgcg gtcaccgtta atacttatag
      301 ctccgtttga aacatcttca cgtgcttgac gtttagatgg ttcaattttt gtggaaacaa
      361 gcagttccac gatattttca ggtgtgcttg aaatttcaac gttcggcatt ttaccgaatc
      421 cttgcgcgat ttcttccgca tttagttctt taatatttcc agagaacaat gcttgcgtta
      481 ttttttgcgc ttctttcaag tcttcttcac tatgcacaaa tcttgtaact tcttctgcca
      541 aacggcgttg tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct aagtcttcga
      601 tttcttcttg agacaagaaa gtaaagaatt tcaagtattt cactacatcg cgatcatctt
      661 gattcaacca gaattggtag aactcaaatg gtgaagtttt cttcggatca agccaaacgg
      721 caccgcctgc agtttttccg aatttcgttc cgtcagcctt cagcatcaat gggatcgtta
      781 aaccgaacgc ttctgcttct ggtccttcta attttcggat taggtctaat ccagctgtga
      841 tatttcccca ttggtctgct ccaccgattt gcaattgcac atcgtatgtt ttatgcagat
      901 gcaaaaagtc gatagattgc aggatttggt aagtaaactc tgtaaatgag attcctactt
      961 ccaaacggct ggcaacgata tcttttgcta acatcgtgtt gatgttgaag tttttaccgt
     1021 agtctctcaa aaattcaagt aaagagattt ttgacaacca gtcgtagttg ttcacaaatg
     1081 tgatatttgc atctttacca aacaattttc tcatttgatt ggaaagtgcg tccacgttat
     1141 gttgcacttg ttccatcgtt tgcaacacac gttctgtttt gcggccgctt gggtcgccga
     1201 tcgttcctgt tccgccgcca atcagaatat atggatgatg gcctgccaat tggaatcttt
     1261 tcatcatcat aaatggaatc aaatgtccga tatgcatcga atcaccagtt ggatcaacgc
     1321 cgcaataaag tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct tcgtttgttt
     1381 gctgattaat ggcgtcgcgc caagttaatt catcaataat attcatttta ctgacctccg
     1441 tgtagttttt ttatgaaagc aggaacctgc tagcatgtgt aattaattat ttcttcagga
     1501 taggatagga aataaaaaaa gtcccttggc atataagcca agggacgaaa tctcgcggta
     1561 ccacccaatt tgtatgtcta gatatccaac atacctctcg aacatttaac gctgtcgcgt
     1621 ggcaatcact gccaaagact cctgaaatgt aattcgcttt aaagcatgta ctggtttgca
     1681 gcgaccacca gctttctgaa acattggctt taacaactac ttcgtttcga tcttcgtctt
     1741 gttattatta atatgtgtct atcatagagc gcaacttttt ctttgtcaac agaaaaagga
     1801 tatttttttt aaattcctca ttttttcttt tttgaatccg ctctaattcc gaacatttta
     1861 ttttcaatta ttgttaaaag aaataacttt ttctgtattt tttctgcaat ttatcgagaa
     1921 gtcactttac tttgcctctt attctttctt cgttacaata aggaatggga caagtaaaaa
     1981 aatgaaaaag caagggacga ggtatatgga aaaaaagcaa aaaattattt taggagccac
     2041 ttctgcttta actatcgtta ttttaattct aagtttgttt tttgtcatcc gatcatctac
     2101 aaaaaaagaa ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag aaccgacaga
     2161 tgataagcaa caactagtta ctcaagcaaa aaatctggct caaagctacg attatgacca
     2221 agcgattgcg ttattggaaa aagatagtaa gaacggatct actgaaacac aacagcttct
     2281 tcaaacacta aaaaaggaga aagaacaact gatcgactgg tctgatccta ctcagatctc
     2341 tcatgtcttt ttccacagct tgatcgtgga cccgcaaaaa gccttccact ctcaacaggc
     2401 acaaggatac aaagattata tggtgactgt tgaagaattc aaccgttcga tcgaacaact
     2461 ttatcaaaat gattttgttt tagttaattt gaatgatttg atccaaaaag acgaacaagg
     2521 aaatctttcc tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg ttctttcaca
     2581 agatgatgtc agctattatg aatatatgga taattccggt tttcctagcc gattggtcgt
     2641 atcaaataac aatgaaatca aaaatgtcta taaagagaaa gataaagaag aaatcggtga
     2701 ctttgacatg gttcctctga tcgatgcctt tgtcaagaaa catcctgatt tttcttatcg
     2761 aggcgcaaaa ggtaccttgg ccctgacagg ttacaacgga gttttaggtt atcgaacatc
     2821 aaaatctcaa tatggagaca atgaaaaaac aaaaaaagag attaaagaag ctaaaaaagt
     2881 ggcggaacag ttgaagaaag acggctggag ctttgcttcc catacatggg gtcacttgaa
     2941 tatgactcaa gcttcactag aagatgtaaa aaaagacaat gaattgtggc agcaagaagt
     3001 tgcacctatc ctaggaaaaa caaatattct gatttatcca tttggtgcag acatcagcga
     3061 ctggcagcct tattcagcag aaaatcaaaa atttgcttac ttgaagagtc aaggtttcga
     3121 tattttctgt aatgtcgatg cttctacacc agcttgggga caattaggta cggatttcta
     3181 tcgaaatgct cggatcaata tcgatggcat tcgttttcaa tctgatttag acggaaaaaa
     3241 tcctattctt gatccattca tcaatgtcca tgatgtctat gacaaagaag cacgcgaatc
     3301 tgcttgattt tacgtcattt atgaaccaaa aaagccgaac tgacatttta tcgtcagttt
     3361 cggctttttt atgctttatt tcttcttcgg tgcgattccc ttgatcagga cttctgtcca
     3421 ttctgctgta tatggaagca gttttgcgta tatctccggc ttagaaattc cttctaccat
     3481 tgcttgaaaa tagaggatca agaactcatc cgaatatttc aaatccactt ttccttcttt
     3541 ccgccctcga tggaaaagat caagcatgat actgaaactt tcttgcgtat acgcatccat
     3601 cgcgtcagaa aattcttttt tttctttttt tgtatagtat gtcatcaaat cgatataaaa
     3661 ttgtttgttc actcgattga gataacggat tttgttttca ctcatggcaa tcagtgtttc
     3721 ttcaaatgga agattttccg caatcaacgc tttcgcttct tgccctaaat cattcataaa
     3781 tgcaaaaaat acttcataaa tcagcccttc cttgctgcca aaatatttaa aaatcgtcgc
     3841 tttacttacc tgagcgttct cagctacctt atccattgtc actccagcgg ttcctatgtc
     3901 tgtgttcatc aagtcaaaag ctgcttgtaa aatctctttc tttttctctt gtgctctctt
     3961 ttcaaaaccg ttcatttgta gagcctcccg taattttaaa cttttataat atatatagtt
     4021 caaatttatt ctgaggttta ttataactat ctattgagaa agtatcaagc tagcaattcc
     4081 ttgtttatga attcattgac agaaatttat aaaaagaata ttatgtacta aaataataaa
     4141 aaaagtttta taaaaaggta taaaaccttt ttattaaact atttaatctt gtttagtata
     4201 tatttttaga aaaaggagtg gggtttttat ggcagaaatc gtcaaagtca cacatttgaa
     4261 aaagagcttt ggtcgtttcg aagctttaaa agatgtctct ttcaccgtac attcaggtga
     4321 agtcgttgga tttatcggac caaatggttc tggaaaatcc acgacgattc ggacactttt
     4381 agggattata aaaaaagacg gcggaacggc aacgattttt gggcaagatg tatgggaaga
     4441 tagtttatct atccatgaac gtctttctta tgttccaggc gatatctctt tgtggggaaa
     4501 tttgactgga ggagcgatca tcgatctctt catgaagctt catggaaaag ggagtgctca
     4561 aaaaaaagat gaactgattc aacgtttctc tcttgatcct aagaaaaaag caaaaaacta
     4621 ttcaaaagga aatcgccaaa aagtgggctt gattgctgct ttggctgttg attcggatct
     4681 ctatttattt gatgagccta cttctggttt agatcctttg atggaaacga ttttccaaga
     4741 agaagttgaa aaattgaaag aacagggcaa atccatccta ctttcttctc atattctcag
     4801 tgaagtcgag cgactagcgg ataaagttgt gatcatacgc gaaggaacac tcgatgaaga
     4861 aggaacactc gatgaattgc gtcatttgac tcgttcgacc atcactatag aaacagaagc
     4921 agagaatagt cacttagcac aattgaacgg tgtccatgac tttgtgcaaa aaggtcacct
     4981 cgctcaattt gctgctgata acgaagcttt cgatgtgatt ttagctgaag cgtccaaatt
     5041 aggtgtgaaa aaatttgaat ctattccacc gactcttgag gatttattca tgagacatta
     5101 tgaaggatga ggaggaagcg aaatgactga taaattttca cgctggtcca cccttttttc
     5161 tcaaaacctt agccgtgact ggaaaaagat cctgatatgg ctagtcggtg tcactgcttt
     5221 ttctggtggt tttgtccctg cgtttgaaga attgacttcc ggacaaggat tagctggatt
     5281 atacgaaacc ttgcaaaacc cggcaatgat tgcaatggtc ggaccaactc cagcttcgaa
     5341 tgctgctgag tatactttag gagcgatgta tgcaaatgaa atgttgcttt tttgcagttt
     5401 gtttgctgct gtgatctctg ttttacacgt ggtgagccaa acgagaaaaa aagaagaact
     5461 tggtctgatt gaacttattc gttcctttcg agtcggacgt caagcaaact ctttagctgt
     5521 catcgctgaa agtattctga tcaatttgct tttagcaata tcgatcagtt gtacgatgat
     5581 ttttttccaa gcggattcga tcactgcaga aggtgcttgg ctttttgggc tatctgtagg
     5641 tagtatgggg attttgggga gtgccgttgc actgtttgct gcacaactga tgccaagttc
     5701 agccagttcg aatggtctgt ctttagcgtt tttaggtgga ctttaccttc tacgagcaat
     5761 aacagatgta gctgattcct ccctttcgat gtggaatcct ttaggctggt cttatttgac
     5821 ctatccattt acaactaaca actggattcc tttgctttat ttgtttttgt tcagtctaat
     5881 cttgataatc agttcatttg ttttagagga acatcgagat ttgaacaccg gctatatccc
     5941 cgaacgtgaa ggaaaaccgt atgctaaacg ttctctttta tccttgcgtg gtttattatt
     6001 ctatctaaat aaagggatga caatcagttg gcttatcact tttggtttga tgggggcggc
     6061 atacggctct atctacggga atatgcaaca gtttttagaa agtaacgact taatgaaaca
     6121 gatgtttatc caatctggcg tctcaattga aaaaaacttt acaagcacga tcatggtcgt
     6181 cttgatttgt ctagctgcta ttttaccaat tgctagcatc aatcgtttat ttacagaaga
     6241 aacccacgga cgtatgaatc agctgttcgc aacaaaaatt tctaggaacc aactttattg
     6301 gaccaatatg ttactagcta ttctatttgc tatattggca atcctgatta gttctggtgc
     6361 actagggctt tctgctttgg cttcgatgaa ggatgcgaaa gacatggatc tattgatgtt
     6421 tcttaaagcc gggttcaatt tcctgcctgc cgttttagtg tttatcggaa ttagtggctt
     6481 tctattcgga tggcttcctc gaatcggtaa acttgcttat atctacttag cttactcctt
     6541 tgcactcaat tactttggcg gaattttgga tttaccagaa tggttttcca aaacagctgt
     6601 ccctagctgg gttccacgga tgccaaacga ttcttttgat ctttccgtgt tccttacgtt
     6661 atcatgtatc agccttgcac tgttcgtcct tggttctatt ggttataggc gaagagactt
     6721 actagaagga taataaaaaa gcatgtacaa tttcatgcta gcctccaaat ctgggtcaaa
     6781 aatccaacat ttggaggtca tttttttatt tacaagtctt ctaactgctc tttatcaatg
     6841 atccagactt ctcgattctt tctttttaca taaccattct cctctaataa tttaaatttt
     6901 ctagataaag tttcgggggt cgtaccgata aatgcagcaa gttctttcat tttcattggc
     6961 aggaaaaagt gatccttccc tgtcgcttta gctaagtcaa gcagatagac agcgactcgt
     7021 tcttctactt tttccatcat caaaaaagtt gtctgctgct gtacttttgt taatttctca
     7081 acgttgattg ctaaaagttt cagactaagg gcgggataat gcagtaagag ttccgagaaa
     7141 tctttttgtc tcaatatgca aatttccgtt ttttctagtg cttcgccaaa taaagactca
     7201 tttggaacgc ctagcaactg gctctctccc tcatatccgc ccggctctat tactcgtaaa
     7261 agctgttcct ttccgctagc agataattga tacactttca tactgccgga ggccacgatc
     7321 actaattgag gttctccatt cggcgcaaga atctgttctc ctttttccac atagtgatgt
     7381 ttgacaagtg cgtgtacttt ttcttgatct tgctgtacta agtgattgaa caaaggaaca
     7441 aatgaaacac ataaatgatc atgactcata atctctcctc cttctaaaca gctatttcta
     7501 ataaagaaac ataacttccc ttattccaac tgataatttt cttttatcat agcataaacg
     7561 tcaagcgaac cagataccag caaatagagt ggatttcttt ttgtcccttg ctttattttc
     7621 cttcttttga gaccttaagt aattgattcc tatcaatttt ttaaatattt actttttgaa
     7681 taatcaaaaa caaaccttca ctcgcaagcc gagagtaagg tttgtttttc gcattgctta
     7741 tttcgtgttg tctattgaaa tttttccgca cctagctttt gatatagtgg tgctagttct
     7801 tgtaaggttt cagcggttat ttgctcaacg tgttcgattt cttgcggata gaagaatagt
     7861 ttacccattt gaaaggttcg gtctctcatc gcttttttca actcttcttg tttctttgta
     7921 tcggacaaat agtcagacaa agctaagtgg tcgtcaagtt catgttctgg ttgcggccat
     7981 atgtaataat ccttgatatc gactgttttg ctccactctt tcagtatcgc aagcaactgg
     8041 ttatacgctg caatgctacc atatcgttct tcgcttttat aatgttggta catcaaatag
     8101 atttggtatt gtttttgatt caacaaaact gctagacagg cattctcgtc ttgacgagat
     8161 tcgcttcggt acgcgctcca aaaatgactg cgtaaattcc agccattcgt ccagctttcg
     8221 attttaggtt tgctcatgcc gtacatatca ggcaaaagtg cagctgtctg gagctgaatc
     8281 tctttccatt tttgccagtg ttctttatat tctttcttga tttgttcaat cttatctgtt
     8341 ggatggctct ctttgagctg tttgaatgcg tagattggtc gatcaaacaa ctgataatct
     8401 gattttccaa gttccattgt ttcgcctctt tccgttatct tttgtattct acctgaattt
     8461 tgcgatatgg acaaatagag gttgtgaaaa agctgttctg catcaagtca taagaacagc
     8521 caaacaaaaa actgcgatga gcatcaccgc agttttattg aacttaaggt tgattgatca
     8581 catctaaaag cgttgaaaat aaaatggcac tagaagcagc tcctgggtcg atatgtccga
     8641 ttgcacgctc tcctagatag gaagctcgtc cttttttagc taccaagact ttggtgtgtt
     8701 cttttgcctg gtcaatgatt tcttttgtta aattccctgc cttcaatgcc tctgtgactg
     8761 gcagccaaac atctaacatc gttttatcgt cagcttctgc ctttcctcgg ctttgaatac
     8821 ccgctgttcc ctgctcgatc acttcgccta atttttcttg agaatcaata gaatcgacct
     8881 cttttgtcgc tttcatcatg ttcatgaatg cagatccgta taacggacca gaagccccac
     8941 cgactttcga tatcatcgcc atagagaata ctttgaatgt atcgctgatt gtttcaggat
     9001 gatcggcttg aaaagcttct tcataagctg aaacccctct tcccatgtta tttccatggt
     9061 ctccatcgcc aatcggggta tctagatcac tgagatattg tttgttctga ttgattgcct
     9121 ctgcaaattg cgtgagccat tcttggattt cttgaacggt tagtttcata ttatcaccct
     9181 tttatcttga gattttttac catgaaatcg tctgtacatt gctttctaaa gaatctttcc
     9241 attgtgtctc cgtcaagtcg atcagtgtta gagaaagacc ttgcatatct agtgaggtca
     9301 taaaattacc gacttttcgg aaaactacat tgacgcctct ttcttccaac agagctaaaa
     9361 catcattcat gaacacgaac tgttccatca aaggcgtccc gcccattccg ttgactagta
     9421 cacctgcttc ttttggtaaa ttcgagtaat catctaaaat cttagtaaca agttcctttg
     9481 ccagtacttt agaaggctgc atcttttctc gacgatagcc tggctcaccg tggataccaa
     9541 caccatactc gatctcatct tctcctagtt cgaatcctgg tttccctact tctggtacag
     9601 ttgctgctcg aagcgctaga ccgatcgtct tagttgcctg tacgaccttt tctcccagtt
     9661 ctttcaactc agctagggaa gcaccgtttc ttgctgcatc gcccacgatt ttgtgtacaa
     9721 ggacagtacc tgcaactccg cgttttccag cagtatacgt actattttca acggcgatat
     9781 catcatctac gatcacactt tctacttcaa tatcgtccat agcagctaat tcctttgcca
     9841 tatcgaagtt caatgcatcc cctgtataat tcttaacgat cagcaaaact ccttttccac
     9901 tatcggcttc cttaatagcc gtctgaatct gatctggagt tggagaagta aacacatcac
     9961 caagcaccgc tgcacttaac atcccttccc ctacaaaccc ggcatgggat ggttcatgtc
    10021 cgcttcctcc accagaaacc aaaccaactt gttccgcttt ctgattcttg gctacaacac
    10081 gggaagattc tacacgatga acaagttctg gatagctttt tactaatcct tccagcattt
    10141 cttcgacaat attgcttgga tcattcatga tttttttcaa tttattccca tcctttcttt
    10201 ccggcatttt gtgcttctgt taagatttgc tgagtatcat ctgttatacc ggcagtgatg
    10261 gctgctgcaa aagctccttc cactaaaggc gcatccacca gatgataacg ttgctgctgc
    10321 tcttcttcca acatgtcttt tgccagttcc gagttcaata ccgcgctacc gatatccgca
    10381 aaaacaaaat aactagctgc atcgcttgat tcattgactg cttctacgat tttcatcggg
    10441 tctgatccga tctcgccatt tattccgccg agagaaaaaa tttgcacctc ttcggatttt
    10501 tgcatctgct caatcatttc tttaatacca tctgttactt tttgactatg agaaataagc
    10561 aaaatacttt ttttcacaag aatcttcctt tcttttacat agttgctaac gattggtttc
    10621 gctttcatta atgaacaatt caagtatata ataaatttct aattttggga aaatatgtgt
    10681 agtgcttctt gaagtttgtt atactttaat taaaagagag gagtgatcga atgattacaa
    10741 caacaacgaa cagtgtagag ggaaaagccg tagtagctta taaagggatc gtttttggtg
    10801 aggtcattac tgggatccat gcatttaaag acttggaggc ggggttgcga aatatctttg
    10861 gcggccgctc aaaaagctat gaaaacgaac taatgggtgc aagagaagaa gcacttgccg
    10921 aaatggccga acgtgcaaaa gcattaggag ctgatgcgat cattggcgta aaaatggact
    10981 atgaagtatt aggcagcgac aatggcatgc tgatggtcac atgtagtgga acagctgtct
    11041 ccacgagata agtaggggac atctgtttta ataactaaag agagatggga catgtagtgg
    11101 cactcaagaa ttagattttt cagtctaact tcgaggtact ctaccttccc atcctctctt
    11161 ttttatttct ttttgcggat gattccgtaa gctgctgcga ctaacacacc cgcccctgct
    11221 aaggaataac tgacagaagc tgttttttca tttgtttttg gcaaagtctg acttgttgat
    11281 ttggatggct ctgtcgtttg ctgaactgtt tctgtcgtgc ttccagaggt cactgatttt
    11341 tctcctgccg aatgcaaaat atcttttatt cgttggaaac tagctgctaa gtcagctttt
    11401 tttgcttcaa aatcattagc aactgtttgt gcttcgctga tctttccgtg gattgcttga
    11461 ctggccgctg tgatatccgc ttgagtttga cttagctttc cagaagctgt ttgagcaacc
    11521 tgatttaatt gctgattgaa ttcttttgct tgttggatct tcccgtcaag atctgtttgg
    11581 acagcttccg cttttgcttt cgcttcggca agctttttct caagttgtgc acggtcattt
    11641 cccactttat ttttaagatc agctaaatct tgatcgattg ctgcttggtc ttctttcaat
    11701 ttgttttttg cagcttctaa tgcttcttcg agttcttttc ttttattttc caagtcttgc
    11761 aaatgatcga tttggtcaga agaggtagaa ctatttattg atgtatcttc tgaagatgaa
    11821 atcgtttctc ctgaattatc tgtcggtaca ttggaagtca tttctgtttc cgaggcaccg
    11881 ttttcagcac cagaagagct tatggttgtt gaaggttctg tttgagcagg ttcagaagtt
    11941 tccgcaaaag ctgtctgtcc caataagaaa atactggaaa cagcaattgg ccagattaac
    12001 ggcttcattc tttttttcat cacttttccc atccttacta ctttttaact aatttcttat
    12061 tataagcaaa aatgtttgga agaaccataa acacaacagg ctgtttgtct aaaaaacggt
    12121 gcatctttta tttttttgta cgttccgggc ttttccatgt cccgcttcca taacctaaaa
    12181 tcgttttgac tgctggaatg aaagtcgtca cgatcgttat cgcgttcact atccagaaca
    12241 aaagcatata tagtggtgcg aaaagaaggt atttcaattt tcttcgtcga tcatctacaa
    12301 tcagcgaagc tagcaactgg aaaaatcctg cgaccatttc aaagcagatg aacacaaaac
    12361 tgaatgtaaa agtaatataa atctgttcgt agtttccttg atatacataa tgcccgatta
    12421 atccagcaaa aagaactacg gaaatacaaa agaatattga ccagacaata ctcaatgttt
    12481 gatcaatgaa gatgatcgtt cttccaatat gtttaaaagg atggagcatc acttttttga
    12541 aattcgtcag ccagacttct gttccacctt ttgcccaacg ttttctgtgt cgatatagca
    12601 tcttcaacgt cacaggtact tccatgaaaa agataatctc aggagcaaaa acagataacc
    12661 agtcatttaa ctgatgatcc caagcaatac tgatgtcttc tgtcgctcga tcttgtctga
    12721 atccgcccac atcgatcaag gcttcttttc gatacatcgt atttgctcca ctgtacgcat
    12781 aaagaccacc gaaaacagct gactgtgttc gtttaatgat gcccacgata cttgagaatt
    12841 caaccgtttg agattttgca atcaatttgg tacgattttg gacatccata tttgcagtga
    12901 ccgcagcaat atgtcttgca ccagggcgaa tgaaataatt cacataccta atcagtgcat
    12961 ctggctccgg taccgtgtct gcatcattgc ttaaaatcaa tttccctttt gcaaaggcaa
    13021 gtccgatatt aaacgcgtgg gcttttcctt tatttttttc gatccgaacc acacgaagat
    13081 tagcgtattt tttcatcagt ctagctaata tttctggtgt ttggtccgtt gaaccatcgt
    13141 ctgtcactaa tacctcatag ttgtgataat tcaatttcgt catcaaatac tcgattgtat
    13201 attcgatcac aatttcttca ttatgggctg gcaccatgat cgtgatgaac ggttcgacac
    13261 tcaagggaac atctacccat tctttttgct tatgtttgta taaaaacaca taacaccaga
    13321 caccaataaa ccatacaaat ccgcctaaaa tcggataaga aacgagaaaa aaatacataa
    13381 tagacaaaca agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc
    13441 caccccattt tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga
    13501 gacagaataa aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac
    13561 ttcttcaagt aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt
    13621 tacttttttg tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa
    13681 actgaaaaaa atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa
    13741 aaaagcgtac aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca
    13801 tataaaagga agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt
    13861 taaccagtaa ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc
    13921 tcttgttact tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc
    13981 atacatttca tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa
    14041 acatttctca tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt
    14101 tttatctctt aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat
    14161 cgccgtgtac atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa
    14221 gattacatga tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc
    14281 taatccatat gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg
    14341 ggtgataact gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa
    14401 caattcttca gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga
    14461 tacgatgtcg atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc
    14521 gctagaacgg taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa
    14581 acaacgcata atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc
    14641 tactaaagta aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt
    14701 ataatcttgt acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac
    14761 aagtaaatga cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca
    14821 agcataagct gagccatatc gtttttcata aattctttca aacaactgat gctcatccac
    14881 ccatgccatt tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct
    14941 tgcataagct acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa
    15001 ctgcctatct tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt
    15061 caaaagatga ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat
    15121 caatttagga tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac
    15181 tgtttttcca tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc
    15241 tgattgtttt agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac
    15301 attttctccc gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag
    15361 ctgtgaaacc agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag
    15421 gacattcgaa atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga
    15481 tgtcatcgta tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt
    15541 tttaccactt cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat
    15601 tgtgttttgt tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg
    15661 gcctaagact tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac
    15721 caaacgatct ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt
    15781 ttgacgaacc gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg
    15841 tgaacgccaa tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat
    15901 ataaaaggat tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg
    15961 acttttcaat aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt
    16021 catctgatcg atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa
    16081 gtaactgtca aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc
    16141 tatttttgcc gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata
    16201 cacgttcttc agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct
    16261 ctttttttaa agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt
    16321 ttaacgagta tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag
    16381 aaaatgcacc atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta
    16441 gatagctacg taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat
    16501 ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt
    16561 ccaattcttc tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca
    16621 attcatgcca ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta
    16681 gcatgtttac tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg
    16741 gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat
    16801 ccacaccttt tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga
    16861 tcaacttcag taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata
    16921 catgaatgac ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg
    16981 atgcggcagt cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc
    17041 gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa
    17101 gagaaataag tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg
    17161 cataaatatc aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc
    17221 ctaattcgat tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa
    17281 taactaaatc agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt
    17341 caaatccagc gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt
    17401 ctataaaagc tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca
    17461 ccatgacttc taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg
    17521 caccaacgat cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat
    17581 ctggtacatt tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa
    17641 caactggttt tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac
    17701 cctcatgtac gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg
    17761 gacgcacatc taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt
    17821 cccgttcact gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat
    17881 acggtccttt ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag
    17941 cagcagacat tccacctgct acacctccga caataacgat tttcatctta tttccctcct
    18001 actgttcgat caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat
    18061 ttctttaatt ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg
    18121 gtcttttctt tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct
    18181 tttatatgcc cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc
    18241 aaaagctttt cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc
    18301 attttctgca cctcatttac atttttataa atacctcatg gggtatatta aacaggagct
    18361 ttattaatat gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata
    18421 aaataccgat aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt
    18481 gataataaaa atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa
    18541 aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt
    18601 tctagtattg atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag
    18661 aaacctgata gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt
    18721 attaaaaagt aaaggaatga tttcaatgaa agtcgtttt
//
LOCUS       DACENJ010000033        18344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.033, whole genome shotgun sequence.
ACCESSION   DACENJ010000033 DACENJ010000000
VERSION     DACENJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.033"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(41..1021)
                     /locus_tag="IW748_000852"
     CDS             complement(41..1021)
                     /locus_tag="IW748_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1103776.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(1045..2529)
                     /gene="galT"
                     /locus_tag="IW748_000853"
     CDS             complement(1045..2529)
                     /gene="galT"
                     /locus_tag="IW748_000853"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1103777.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(2541..3530)
                     /gene="galE"
                     /locus_tag="IW748_000854"
     CDS             complement(2541..3530)
                     /gene="galE"
                     /locus_tag="IW748_000854"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1103778.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            3778..3945
                     /locus_tag="IW748_000855"
     CDS             3778..3945
                     /locus_tag="IW748_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1103779.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(4007..5818)
                     /locus_tag="IW748_000856"
     CDS             complement(4007..5818)
                     /locus_tag="IW748_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1103780.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(5815..6180)
                     /locus_tag="IW748_000857"
     CDS             complement(5815..6180)
                     /locus_tag="IW748_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1103781.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(6343..6738)
                     /locus_tag="IW748_000858"
     CDS             complement(6343..6738)
                     /locus_tag="IW748_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1103782.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(6756..7718)
                     /locus_tag="IW748_000859"
     CDS             complement(6756..7718)
                     /locus_tag="IW748_000859"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1103783.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(7718..7930)
                     /locus_tag="IW748_000860"
     CDS             complement(7718..7930)
                     /locus_tag="IW748_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1103784.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(7951..8649)
                     /locus_tag="IW748_000861"
     CDS             complement(7951..8649)
                     /locus_tag="IW748_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1103785.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(8669..9211)
                     /locus_tag="IW748_000862"
     CDS             complement(8669..9211)
                     /locus_tag="IW748_000862"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1103786.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(9343..10347)
                     /locus_tag="IW748_000863"
     CDS             complement(9343..10347)
                     /locus_tag="IW748_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1103787.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(10344..11333)
                     /locus_tag="IW748_000864"
     CDS             complement(10344..11333)
                     /locus_tag="IW748_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1103788.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(11330..12136)
                     /locus_tag="IW748_000865"
     CDS             complement(11330..12136)
                     /locus_tag="IW748_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103789.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            12302..13258
                     /locus_tag="IW748_000866"
     CDS             12302..13258
                     /locus_tag="IW748_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1103790.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(13335..13853)
                     /locus_tag="IW748_000867"
     CDS             complement(13335..13853)
                     /locus_tag="IW748_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1103791.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(13941..14090)
                     /gene="rpmG"
                     /locus_tag="IW748_000868"
     CDS             complement(13941..14090)
                     /gene="rpmG"
                     /locus_tag="IW748_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1103792.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            14318..14764
                     /locus_tag="IW748_000869"
     CDS             14318..14764
                     /locus_tag="IW748_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1103793.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(14959..16854)
                     /gene="asnB"
                     /locus_tag="IW748_000870"
     CDS             complement(14959..16854)
                     /gene="asnB"
                     /locus_tag="IW748_000870"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1103794.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(17179..18153)
                     /locus_tag="IW748_000871"
     CDS             complement(17179..18153)
                     /locus_tag="IW748_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1103795.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         5546 a         3868 c         3141 g         5789 t
ORIGIN      
        1 gctgcggtat cactccattt tcccacagct tttccatctt ttatttcgtt gattctcgaa
       61 taatcaactc tgtttccaca ataatctttc ttgaaaccgg tgctttggaa ttacttaatt
      121 gaagaatcgt ctccactgct agctctccca tccattcggt ataaactttt acagtagaaa
      181 gcgagggcgt tacatatttc gctacactga catcattgaa gcccatcaca gaaatatctt
      241 ctggtaccac taaacctttt tcttgaatcg ctttcagtgc gccaatcgct aatgcatcat
      301 tagctgcaaa aagcgcatca ggaacatgcg gattcgtttt taaatactgc ttcactgctt
      361 gatatcctga ctcgacagaa aaatctgctt cgataattgg accttcgtcg tagccttttt
      421 ctgccaaaac ttgttgaaat gccataattc tgggatcttt tagccgctga tggtttttct
      481 tcgtatattc tgttccagca agcatagcga tatgttgcgc tttatgggag acaaagtgtt
      541 caatgacttt ttgaacactt gacttaaagt cgacaactag agagtcgatt ccttgagaag
      601 tgccgtcaga atctaccaaa agcaaggcat tttgggatcc tttcatttct aataactgag
      661 cttcgtcgaa ttttcctagt gcgatcgttc catctgccgg aagatcacta atggaatccc
      721 acgtttcttt caataaagta acgtttgctt cttctgcctt tttttcgatt cctaggcgaa
      781 tagataaata ataaagatct tcaagctctt ccgtatcgtc gtaccactgg atcaggcgaa
      841 gcgtttgctt agtatttgct gcatttttac gatgtttcgt ataatttaag cgttctgctg
      901 cttcaaaaat tttttgcttt gtggctaagc caacggaaag atcaggatca tagttgagaa
      961 ctcttgaaac agtagcagga gaaactcctg ccaactgcgc aatgtcttta attgttgcca
     1021 tttgtctctc ctcctttttt acgtttataa aatcgcagta aatcgttcaa acgctgcctg
     1081 tcctttttca tctcgcttga atactccagc gtcttccagt acacgtgcaa aaacctgacc
     1141 tacagatttt tgtacgacat cggtgaccgt atctttcgtc aaatcaggat gttctttttt
     1201 taattgttct gcccacggac gatgatattc agccatctgg ttttcttctc ctaataagaa
     1261 atgctctact tctactagtt ctggtttcaa acgaggtggt aaaacagcaa gccccattac
     1321 ttcgatcaac ccaatgttct cctttttgat atgttggaca tctttatgtg gatgaaaaat
     1381 cccatcagga tgttcttctg aaacattgtt gtctcgaagg acaaggtcca actcaaagta
     1441 attgtccctt cttcgtgcaa ttggcgtaat cgtatggtgt ggtgtaccat ctgctgaaaa
     1501 agctctgata gatactgttt catccgaata tgctttccat ttttccaata ctgagttagc
     1561 tgcttcaata agcaagtttt tctctgttga ttgcaaacgg atcacagaca tcggccattt
     1621 cacgactcct gcgatgactt gcggataatc ttctagtttg ataagttttt ctatcggtgc
     1681 ttttgccata ggaaattcat gacgtcctgc ctgataatga tcatgagaaa gaatcgatcc
     1741 tccaacaatt ggtaagtcag cattcgaacc aacaaagtaa tgaggcaaga cttcagtaat
     1801 ccgaagcagt ctagcaaatg tttcctttgt gattttcatc gggcggtgtt gttctgataa
     1861 aatgatcgaa tgttcattgt aataagcata aggggaatat tggaatcccc agctttcacc
     1921 gtctagattc attcgaataa tccggtgatt ggtgcgggct ggatagttca agcgacctct
     1981 atatccttcg ttttccatgc aaagcatgca ggtaggatag tctactttta ccgcctcgcg
     2041 ttctgctgcg atttgttttg gatctttttc tggcttagat aaattgattg tgatttccag
     2101 ttcaccatac tccgactctg ccggaaattt gatgtttttt gcgatggctc ttgttttgat
     2161 gtagtcattt ttcttactta aattaaagaa atactcagtt gcttcctctg ggcttttcga
     2221 atagtgctgt gcaaagaatg cattgacgac tgaaggtggc ggtgtgagaa aatccattaa
     2281 ttgcgcttca aaaatctctt gttctgctgg caattctgaa agaacaccat tttctcttgc
     2341 cttagcgaca agctgatcca ataagtctac agattcttcc ttaactggtc ttacttcaat
     2401 gctttctaga gcatcttctc ctaccatccc taaaagacga ttctgtaaat ataagcggtc
     2461 catttccatc catccgccag cttcgatcgc aagtgtggca aaatcacaaa tcgtttgact
     2521 gattgacata tctcattccc ctattcttca taaccatttg gatggctgac atgccaatcc
     2581 catgctgttt tgataatcgc tttgatatct gtgtattcag gtttccagcc cagaatctct
     2641 ctcgcttttt cacttgaagc gactaatcgg ctaggatctc cagctcttct tggtgctatt
     2701 tctgctggaa tttcttttcc tgtcacttct ctagctgctt ctagcatttc cttgactgag
     2761 tatcctttgc tgctgcctaa attgaagaag ttgctttcat ttccttcttt caagtattcc
     2821 aaagctaaaa tatgtgcagc aatcaaatct tctacttgaa cataatctct gatacatgtt
     2881 ccatctggtg tatcataatc gtctccataa acagccaagg cttttctttg accaagagct
     2941 acttgtaaaa tgattgggac aagatgcgtt tctggtgtat gatcttctcc gatagatgca
     3001 tctgcttttg ctccggctac attgaagtag cgcaaagcga catagcgcat gccatatgct
     3061 tggtcacacc acttcatcat tttttccatc atcaatttac tttcaccata cggattttta
     3121 ggattagctg gtgtatcttc tgtgattgga gactctttcg gctctccata agttgctgca
     3181 gtagaagaga atacgatttt attcacgttg aactcgtgca tgacttcaag taagatttgc
     3241 ataccgtata cgttgttgtt gaaatacatc aatggttttt cgacagattc tcctactaaa
     3301 gagcttgcag caaaatggat tactccttca atcggttcct tttcaaatac ggaacgtaga
     3361 aattctttat cccggatgtc tccttcataa aaatgagcat ctggatgtac tgcttgtttg
     3421 tgacctgtga gcaaattatc taccacagct acttgatacc ccttttgaac tagctggtct
     3481 acagcgtgcg aaccgatata accggctccg cctagtacta aaattgtcat aaaaaacact
     3541 cctttgatta gtaaaattgt gtttgttgat tctatataat ttaaaaaacg aagtttactt
     3601 ttatggtgaa aacaaacaaa gtaaaatttc gtaaattcat atggtaaatt tataataaaa
     3661 catttagtaa acattttcaa ggattttttg caaaggttta caaacaaacg taaaactagc
     3721 ctttttttct cttttgttcc ataatagata taacaaataa taaatgaggt ggattcaatg
     3781 aaaaaatttg tatctggaat attagtagga agtctggcaa cagccgctgc cgttgctggt
     3841 ttggttgctt ctgtgaaaaa aacagtgatc gatccaattg acgaaaaaga agcaatgatc
     3901 gaagaaaacc gtaaaaaagc aatgcgtaaa cgcgtttctc gttaataatt atttaaaaat
     3961 acgcatgaag actttctaaa aaacagataa gtcatcatgc gtatttttat ttgtcagtca
     4021 atataagttg tgctatttta tctgcctgtc tcagattcca tggatctgta taaggtctag
     4081 ctactgctgc agttagccaa tccacacctt caacaggtat tccatagcaa taaaagtcag
     4141 actggatttc ccctgaagca tcaatcacgc gattcgtacc tcgctcaaca tctatagctt
     4201 ctgaatgata acttcctgta ttatctgaaa atgaaaaagg gcgaacaagt ttgttttcct
     4261 tcaaattttt tatgacagga ttacttgaac gagaaaagct tgtttgtggc aatcgagctt
     4321 caatcaaata gcgactgctg atttctttat cttgtagaga aataacatag cggttgtgct
     4381 tttgctgaat atcaaatggc ggttcgatca aatgaaaaat acccgcatct atcagtgctg
     4441 ctaattcttt cgtccgaatg attggcggac cgatcgttaa aaagctattt agatgagtaa
     4501 accatcccca tagtatttct ttgtattcct tagcagaaaa aacgtgccat tcaatagcct
     4561 gccggattgg atcacgccag tcttttaatg catcaaatgt tgaagcaaat gctcctgtgc
     4621 aattgccttt ttccgcttct tttatctgcc cttcaataaa tattcggcta gcttctgaaa
     4681 agggctcatt ctgataggca agaggagttt ctaaatacga ccaatcccat ctataaggga
     4741 ttagtgctgg atgctttccg agacactcct ctgaagagag ggagagaaaa tcatgctcaa
     4801 aatttttctt tggaatcggc aagtgatatt cttcgatgat tttcttgtaa taaaacaact
     4861 caacgtcttt tttcagatag tcaaaaaatg tttcgccagt caaaaatccc gactgatgcc
     4921 attgctccag gttttctttt gttagtaacc gtggccatgc cattgctccg ccttgctttt
     4981 gatttcttcc tcgagggtaa taagggagcc cccttcttga tccactgtat acaatcggtt
     5041 ccttgcctga tggatgatag accagttttc ccttttcgtt ctcgaagcgc cctcctcttg
     5101 atactgtaaa caatccgaca taatcaaaaa aacttaagcc tagacctctt aaaaagacat
     5161 tttctttagg cggtatcttc gaaagatcta catctgcagg attagaagga agctgataca
     5221 gtaaaacatt tttctttgcg tagtctaccc acactctttc ttcttctgtc tcatcatttt
     5281 cccagtgacc gcttgctaaa acaagctgat cgatagaata gctttgctga tctgtctgta
     5341 aaagataacc atcttttctt ttgcttactt tttttactaa agaataaaca aactgaatag
     5401 aagctggaaa ttcttcttgc aactttgaga aaaaccagcg ttgatagagt ccgtataaac
     5461 atcgagtaga ttgctgatct ttttccagac gattgatttc ttctataatt agttgcttat
     5521 ttttggttac cgtctgttcg atataatctg tagcaggtcc tttactccat tgaaacaagt
     5581 tgggtccctg tgttaccaat cctccactgg acaatgtttc atctgtaaaa agcgtcactt
     5641 gttgagaaac agaattcatt aataactccg gtggctgatt caaccgccaa actcgacctc
     5701 caggaccatt gggatcgaaa agaaaaagtt caagagaatg tctgtcctga ttattatgga
     5761 gaatcctttc tgctacactt aatcctctag gtccggcacc aatgatccct attttcattt
     5821 gcatgcctcc atttcgataa attttttcaa taaaacagca tggttctgat gttcattttt
     5881 tgcaccataa acaaaagtaa tgactggatg ttctttcata agttgactga gtttaaaaat
     5941 agcttgcttc ttttcttctt cgaaaatcaa ttcttgttgg tatttctttg caaatgaagc
     6001 aaatcgttct ggtacatggt caaaagtttt gcgtgcttca ttactaggtg caatttcttt
     6061 caaccacaga tctaactgcg ctttttcttt tgaaattcct cgtggccaga atctatccac
     6121 gagtattcta tagccatcct ctttttcagc atcaaggtaa acacgtttta atttgatcat
     6181 agttgtttcc ttctttctac cttttcgctt agcttatata tgtagataat agcacaaaat
     6241 aaaaaaaatg aacggcacaa ctatccattg cgccgttcat gtgatcttgg tcaaaatcta
     6301 atgattgatt cggtttttaa agctgtcata caatcatctg ttttatactt ttttactttt
     6361 gatttttcca gaccatccgt catatccacc cttaagtaaa taaatctctt gataacctgc
     6421 tttacgtaat ttatttgctg tacggatgct caaacttttc ttttgatcgt aaatatagac
     6481 aggctggtct tttcgaatag atcctaatga atctttcaaa acagtgtaag ggatgtttct
     6541 tgcacctaaa atatgcccag catcaaacac atctttttcg cgaacatcga ttacttgtgc
     6601 ttttctcatt ccttctcgaa attcttcttc tgtcaaagtc gttgctgaac gtttggccat
     6661 tactcgtaga tagacttccc aaaggatgat tgcaagcaca atcaatagca aaatgatatt
     6721 aattacccat aaaaccatgt aatgcctccg tttttttaag caaatttcaa ggctaatgag
     6781 gcagcaccaa tgacaccagc ttcattacca agctctgcta atttgatttt tgttgattct
     6841 cttacttgcg ggaaagtaaa ttctttaaaa tatttctcta cacggctacg taaaaattct
     6901 cctgctgccg atactcctcc acctaacacg atacttgacg gatttaatgt attccccaag
     6961 tttccgcaag ctaaacctaa gtagaagcat acgcgatcaa caaccatcaa tgcaaacgga
     7021 tcgtcttctt gagccatttc aaagatatct ttacttgtta cttcttctcc gttgtctagc
     7081 attgctttca gttttgaatc ccctgcgtat tcttctgcca agaaacgacc taggcgtacc
     7141 acacctgtag cgctagatac agtttctaaa catccgcgtt taccgcaagt acattcgaac
     7201 ccttcaggat ccacagtgat atgtccgatt tctcctccag ctccgctcac gccgtgaatc
     7261 aaatttccgc cagcaacgat acctccgcca acacctgttc ctagagtaac gaatacaacg
     7321 tctggatcgt tttctccagc acctttccat cgttcaccta aagcagcaac attcgcgtca
     7381 ttatcgatag cgaatttgat tcctgttcct gcctcgatca gttttttcac aaattgaact
     7441 tctgtccagt ttaagttata cgcaccaata accgttccag cttcgctgtc cacgcttcct
     7501 ggggtaccca tgccgattcc gataaagtct tcggctttca tattatataa attcaaacga
     7561 tgattgattg attcaatgat ttcaggaacg atatgcttac catcatccaa aatattggta
     7621 tcgatgctcc atttttgctg aatctctcct tctggtgtta aaattgcaaa ttttgctgtt
     7681 gttccaccta aatcaatccc aataatcttc ttgtccatta caaatttcct ttctttcggc
     7741 tttcttcaat tcggtgctct ctttttaata tgcgccacgc tgtcatgtat gtatcatgat
     7801 caatcaaacg gctatcatac agattacgca attcgatagt cattaattca atgtcataga
     7861 tgcgtgcacc cacatagata aagatgccaa attttttgaa cagctgctgt acatcatata
     7921 agctctccat ctttaagcac ttccttcata ttatacaagt aaaccatatt tttttaaacc
     7981 cagtaaaata catattacaa aaagaaatac aaaaactagt gtagaaagaa tcctttggtg
     8041 tatcgaatag cttgtctgtt gtttcggtag acccaaaaca ttccctaaca ctaatccgcc
     8101 aattagccca ccgacatgtc cccaaacatc tacagaggta tccagaagac caaaaacaaa
     8161 tgtcaacaca ataaataaaa gaaaatgtct gactagctgc tggatcgctg ggttatgttt
     8221 aaaatgaaac cctaagataa acaacgcacc gaacaaaccg aatatcgaag tacttgcacc
     8281 accagacaga acattcgctt cgttaaaagc aaagcttgct gtatttccca ttatcccgct
     8341 gaacatataa atcaagaaaa aacgccaatg gccatagatt gcctcgattt gctgccccat
     8401 aaaatacagt atcagtgagt tcattacgaa atgcatcagt ccaaaatgga tgaagattgg
     8461 tgtcactaaa cgccaccatt cattgaagtg aacaagataa ggaccataca ttccggcata
     8521 ataagggatc tcaagtcgcg gaaataattc catcatgagg aagaccgctg tctgtatccc
     8581 taaaaaagca taggtccata acggtcgtct taaccatgca cgcaatttta tttgctgttg
     8641 ataattcatg aaaacctccc atttcatttt agtcaataaa caattgttgg atcttctgat
     8701 caaagctctc cggttgccat tgttcattca attgttcacg aaaaacaagg ctacatgttt
     8761 ttttatgaaa attatttaaa taccggtcgt aataaccacc gccaaaaccg attcgataac
     8821 cggctctatt gtagataata cctggtacaa tgaggagatc gatcatctcc ggcaaataga
     8881 gcttctctga ttgtggctca agaacaccaa aactcgaacg tacaagaacg gactgttcat
     8941 ccatttcata aaacgccatc tgacgtttcg gcaaagtttt aggtacaact attttctttt
     9001 gttctttttc tgctcgttgg ataaccggct gtgtatccag ttcaaattca ttcgataaag
     9061 tcgtccctat cacttttgct gacttccagt actcgctggc gaatagctgt ttatagattc
     9121 gctgttcttt ttcctttttc ttgccacttt tggccagatt tttcagcttt tcgattcctt
     9181 tccggcgtaa tcttactttc aattggtcca aaaactcacc cttccttctc tcataaaatg
     9241 gtataggaaa aaaagaaaaa tagcaatcga actttcaaat aaatgcataa atagaataaa
     9301 aagaagacct agaaatgatt ggccttcctt ttttaatgaa tttcaactgt tcagcgcgtg
     9361 tttttgtctg ataaaaagtg catacagaaa atatggcgca ccaaaaaggg caatcaaaat
     9421 tcctgtaggt acttccccac ttagtagaat cactctagca accatatctg cactaagcag
     9481 aaaaatggaa ccgaacaaac cgctcagaac aaaggtccag ttattttctt tacgtacgac
     9541 tagcttcgca gcatgaggag ctagtagtcc tacaaaagat aaactacctg tcaccgctac
     9601 actaccgctt gccaaagcaa cagctaacaa caaaaggatt ttttgacttt tagccacgtt
     9661 aaggcctaat ccttgcgcgc gttcttgacc aaatgatagc aatacaatcc tctgtgcata
     9721 aagtcctcct agcggaatta agataaccgc ccatggaagc aatgcccaaa cataaggcca
     9781 gctcgcaccc cagattgatc ccgctaacca tgcattcaca aatccataat tttccttaga
     9841 taattggatc gtgcatagca atgttaatgc agaaaggccg gcattaacgg caataccgga
     9901 caataagatt cgattcatac ccaaaaagcc atttcgatca taggccgcaa gatagacaca
     9961 gcaagcagct aaaacaccac ctagaaaggc tacaactagc aatagaaacg gcagcgaata
    10021 gtgcgaaaag aaacctaaat aaagcatcac taaaaatcct gctccggcat taatgcccaa
    10081 tatactagaa tctgccaatt catttcttgt tactccttgc aagatataac cagaaacgcc
    10141 aagacaagct cctgctaata aactgatcag taatcgagga actcgaaatt ctctaacaat
    10201 cagcgccaaa gaagatgatt ctttccctat gaacgtggca aaaagctgat ccaggtaaat
    10261 aaaaggcgta ccaatcatta aactacctat agacaggatg acaagtacaa aaagcaagaa
    10321 atagaacgtc caatatattt ttttcaccgt tgttccctcc ttacttgtat cagtaaaaac
    10381 ggaacgccaa tcaatgcaat gactaatcca aaaggtgttt caaaaggggg attgatcatc
    10441 ctcgctgtaa aatccgccaa cataaccaat ccaccgccaa ctaaaaaaga gacaggtaga
    10501 acataacgat agtccgttcc aaccattccg cgaacaactg gcggaaccaa cagcccaata
    10561 aatgaaacag gtccgactaa cgaaacggca atggctgcta acactgcaac tagacagaaa
    10621 acaatcaacc gcgttcgttt aggatgactt ccaagagcta atgcatgtgt ttcactcaaa
    10681 tgaatcaaag atattgcttt tcctaatgaa aaagaaacca aaacagcaag tatatataaa
    10741 ggaaacaata ctgctaattg tttccatgtg atattggcag ctccgcctac aaaccaaaaa
    10801 gcaagatcct gctgcagatt aaagaattgg gtaagtgctt ggctgatagc agtaaaaaaa
    10861 gaactaatag ctacgccagc taaaatcagc tgtaaaggat tcatgcccat tttcgaaaac
    10921 tgcaaaaaac caaatacgag caaagttaca gcaaaagaac ctgccaatga cattagaaac
    10981 acagaaaaat cagaagcttt aggccaaaaa gcaaaactaa gggccattgc caacgaagca
    11041 cccgcgttga ttcctagtaa gcctgaatct gctaaaggat ttcttgtcac tccttgcatc
    11101 aatgcaccgc tcaatgcaaa acaactccct acaagaaatg ctcccgccac acgaggcaaa
    11161 cgaatatttc gtatgatttg gtgactttgt tttgatctat caaaatcaaa caacgcctgc
    11221 caaaaatctg cttttcccac agtcgctgcg ccatttgaaa gagcaacaaa aaaaataatc
    11281 aaaagaacaa aacagaccaa aagcaggagt ctatttttct tcatagtcct cataattgat
    11341 cctcctgctc ttttggcaac aaatcatagg ttagaaaaac tggtttattt gttcttggaa
    11401 gcgtgacaat cgttgcatca atctcaaata tttttttcat acactctttt gtgatcactt
    11461 cttcaggtgt accttctgtg atcaacttac cgtttttcat tgctaataaa taatctgaaa
    11521 aacgtgaagc atgattaatg tcatgaatcg acatcaagat cgttgtttgt ttcatttgat
    11581 tgatttcagt caataactgt aggatctcta attggtgtgc cggatctaaa aatgttgtcg
    11641 gttcatccaa gatcaagata tctgtttcct gagcaagcgc catagcgatc cagacacgct
    11701 gggtttgtcc accagacagc gttgcgatat cccgcatacg aaatccagct agacctgtag
    11761 catccaacgc ccactcaatt gcttcatgat cttgcgtgtc catgccactg aacagttttt
    11821 gataagggta tcgtccgtaa gatactactt cttctactgt catacctgta gggtgttcac
    11881 tagtttgtgc taataatgca attttttttg caagttcttt tgaactgtat gtagagatat
    11941 cagagttatt gacaatgatc tttcctgatt tcacaggtag tatacgggta aaacttttca
    12001 ataaagtaga ttttccactt ccatttggcc caatcaaact agtgattttc ccttcaggaa
    12061 tcgtaacaga catgttttca atgatttttt tgttttcgta gccggctgca atgtcagctg
    12121 ctcttaatct cgtcatttca cccatccttt cgctgaaaat cattctcaat tatgacgctg
    12181 tttcaacatt tcgtcaagcg ctactttcat atctatagcc aaatgaagcg gttgacagcg
    12241 ttcgaaatat caggaataat aaccaatgag aaagattctc aattatgtta ggaggaaaga
    12301 aatgaaatta aaaacaaaag tattcacatt atctagcata ttgctgctgg ctggagtact
    12361 gagcgcttgc tccccctctt cagataaaga aacagctaca actgccaatt cgacgacttc
    12421 atccaatagt actcatacag tcacagatac acttggacat aaagtagaga ttccaaacgc
    12481 gcccaaacgg atcatcggca gttacttaga agactatctg attgcactag gggaaaaacc
    12541 tatcgcccag tggactgttg gcagtggttc cattcaagat tatctgcaag atgatttaaa
    12601 ggatgtccca acgatttctt atgaccttcc ttatgagaaa gtgttaagct ttgagcctga
    12661 tttgctgttg atttcttctt ccgctactgt cgaaggcggg aaatacgaac aatatagcaa
    12721 aatcgcgcca acttatgttg taaaaaacgg aacagatgtc acatgggaag aacaactaaa
    12781 agatgtcggc aaagctttgg caaaagaaaa agaggcagaa aaaatcattt ccgattatca
    12841 aaaatccatc aagaaaactc gagaagaact agcagataaa atcgagaaca agacagctgc
    12901 agtgttgtgg gtgaccaaca actcagcctt tatggtcagc aaaaatcggt caagtggaag
    12961 aatcgtatac gacgatctgg aatttggtgt tcctgatcta gtggaagagg tctccaaaaa
    13021 agccacttct gactggtctg ctgtttcctc tgaaaagcta gctgaattag atgctgacta
    13081 catcatcctt gtgaacagtg acaaagatgc tgccatgttc aatgaaccaa gctggaagaa
    13141 tctaaaagca gtgacagaca atcatgtact gcaattcgga cctgaatcta gttggctata
    13201 taacggacct attgccagtc aggaaatggt caatgatatc aaagaaaaac taaattgatt
    13261 ttaactaaaa aaagcatctt cacgattagt cgtgagatgc tttttttctt attaacaaac
    13321 tagacgcata gattttattc ttcttttgaa gcaaccctat tttttactct ttcccatttt
    13381 tgaaaacgat gggcatatag attttcttga tccgttatcc cctcatactc ctctataagg
    13441 gcgaagtctg aaaaatcaat attcccaata tacgtatcgc cttcaaacgt gctatcgatc
    13501 aatgttcgcc agatccgacc agtgtaagga agaagttctt gaaaaatccg actaccaccc
    13561 gaaacataga tatcttctga gatcgtttga ctatacgaca aaagttcatc gatcgaatgc
    13621 attacttccg cattttcttt cgtgcgaaaa tttggttgcg ttgtcaacac gatgatatgg
    13681 cgataaggaa gtgaaagatc ccccattcct tcataggtct ttcttcccat cactaaaatt
    13741 cgatccattg tgtgttcgcg aaagaaacgc atatcatttg gcaatcgcca tggtaacaaa
    13801 ccatttttgc cgatcagacc atttttatct tgtgcccaca tggaaataaa catattatcc
    13861 gctccttatg attttagatg ttttgagata aaaaaagtct caattctttg accagcaagg
    13921 aattgagaac tttaaaaaca ttattttgtt tcacggtgca aagtaacttt gcgttcgcgt
    13981 gggcaatatt tttgtttttc caaacgatca ggattgttac gtttgttttt gcttgttaga
    14041 tagttacgtt ctttacaaga agtacactct aaagtgatgt ttacgcgcat gtttttccct
    14101 cccattttta caagaatcct ctaagatgac tctcagactt gattatctta ccatgatttt
    14161 ttctagattg ctagtgtttt tcacgatttt gttaagtaaa actacttgac gataaataca
    14221 acggttttac ttctcaaaca aaatcttttt ctttttgtat aaataccctt acacttgttt
    14281 atatagatta tgttttttaa taaaggggga tactaatatg ccatggaaca tgaaggactt
    14341 tcctgcatca atgaaaaact tagataaatt gacaagaaaa aaagcaatcg acatcgcaaa
    14401 tgcattatta gatgaagggt accctgataa ccgtgcgatc ccaattgcca ttgatcaagc
    14461 aaaaaaatgg tatgaagatg ctagtgaatc agaaaaacga actttcgaaa aagaaaaaaa
    14521 tccaagcaag acggatgaac acgatacgaa tcctcgtgct ggaaaactgc ttgattcaga
    14581 tgtgatcgta gaatatgagg aagaacaatg gatcgtgaaa tctaaagggg caaaaaaggc
    14641 aagcaaccat tttgatacaa aaaaagaagc aatcgaaaaa ggaaagcaag tagcacaaaa
    14701 caaagaatca actttgttca tttataaaaa agatgggaca aaagaaaaag aaatcgatta
    14761 ttgaacatat gtaaaggggt tgtgacgaaa atcgtgtcac aaccccctta tgttcgaaac
    14821 aatggtgttc ctctgccaac tccgaaccat aagtcataat atctataaaa cgggaagagc
    14881 tgttttttga aattgtttct tatcactcag tgttaaacgg taggagaaaa cctctttttt
    14941 attgatattt gattttcatc aaacagtcac atagtcgatt gcttctgctt gcggaatcgt
    15001 ttcatctaca aagtagactt tataccatgt caagaaactg aagattgtcc agatttttct
    15061 ttgctcttct ttacgtcctt cgtgatggtc atctagcaac ttcatgattt tttcttgatc
    15121 aaaaaattct tttgcaaaat ctgcttcgaa taatgctttg acttgttcgt agccgtgttc
    15181 ttctttcaac caagctttga ttggcacagg gaatcctaat ttcacacgat tcgaccattc
    15241 ttccggaaga tgtttgttcg cagcttgtct gaaaatatct tttgtgttgt tttgattcaa
    15301 taaaaactta cttggtattt gttgagccaa tttcatgact tcgatatcca ataaaggtac
    15361 tcggacttcc aaagaacttg ccatagaaag tttatctgcc tttaacaaga tatcttttgg
    15421 catccattga tgaagatcga catattgcat cttattgact tcatctttga ttccttctgt
    15481 tttctcgtaa tgaacagtca tgatttcatc cacagatggt gctgtttgat attttggtgt
    15541 taacacttct gatgcttctg attcttcgaa cactcgagca tgaccgataa aatattcttt
    15601 tgcaggagca agtgattcat ataaatggat ccgtccatgg aaatttttca tttttccgat
    15661 tttacgtcct aaattatagc gtgttccttt tggtaatttt ttcaatcctt gagcaatcac
    15721 acggatgaat tttgaattcg tatggaatcc atacgtttgg tatcccgcaa acagttcatc
    15781 cgccccttct ccagattgga ctacccgcac actttgtgaa gcaagatttg ctaaaaagta
    15841 caaaggtacg caagaaggat ttgaatcagg ttcatctaag tgatactgga tcaaagggaa
    15901 tgctttgaac gacatgtctc cgtcgatcac tgctgctgta ttatctaagt ccaatagttc
    15961 ggttagtttt ctggcttcaa tcgcttcatt ataggtttta tcatcaaacc cgattgagaa
    16021 agaatgatct ggtttcaata cagaggttac ataactggaa tcaaccccgc tggataagaa
    16081 agaaccaact tctacatcac tgaccgtatg tgcttcaata gaagcttgaa cggtttcgtc
    16141 gattttttcg atccattctt gtcgggaatg cgtttctttt gtttcaaaat ctgcatccca
    16201 atattgctga atgtctattt ttccatcttt atacgtataa taatggcctt caggcaaacg
    16261 atagacatct ttgaagaatg tttcgccgtt taatggagaa tattggaaag tcatataagg
    16321 tttcaacgct tctttgttca attctttgtt aaaatcagga tgaggcagaa aacttttgat
    16381 ttctgaacca aacataaatg ttccgttcat ttctgcataa taatatggtt tgatcccaaa
    16441 gtggtctctt gcaccgaata actcattctt ttcattatcc caaatagcaa aagcaaacat
    16501 tccgcggatc ttttgcaata attctgttcc ccattcttcg taaccatgca aaaggacttc
    16561 tgtatctgca tgtgtttgaa aaacgtggcc agctgcaatc aattcttctc tcaaaggctg
    16621 gtaattatag atttcgccat taaaaataat gatctttgtc cgatcttcat tgtaaatagg
    16681 ctgagtaccg ccctctaagt caataatact taatcttctg aatcctaaag caacatgttt
    16741 atcgatatat tctcctgaac tattaggtcc acggtgaacg attcgatcca tcattgtatt
    16801 aataattgtt tttttgttgt ccttgtcgtt aacaaaacct acaataccgc acatttaact
    16861 ttcacatcct aatataaaat tactgtttat ttcattcata tgcatcttat catagaataa
    16921 atagcggacg aatattttct ctcttacaat ttcgtcacgt gaaaacaaaa gatgaaagtt
    16981 tctgttttta cagaaaataa agtcccaata tctaatttac catgttttct tttttgaggc
    17041 taccttattc tcttcgtgtt aaaaaaatat tagtggatgc tatttccaaa aaaaccagca
    17101 aaaaaagaca cgacctaaag atcgtgtcta attatgactg tatcagaaaa tatgatacca
    17161 aacggatcta ttcattaatt aattgacata gtttatctgt tgctctttta tgactggata
    17221 actgaccaat tcatgacgat ccaagtcttc tttattcata tttacagcag taatttgact
    17281 gtcttcatac gtacgataat aataaatccc tttatcaacg ttacagcaag aagaataaat
    17341 cgtatattcg tattttcttt ctccagtatc gcatactcct ttttgttgct cgactgagcc
    17401 tagtatatga aaaaattgac tgatactttc agattctgaa tcacctgatg cagcattcat
    17461 tttagtaaat gtggctttta caaatcgaga aacagaagag agatctccag gtaagcccaa
    17521 tcctcccatc ccacgactat aaacattcaa gctgatctgg tttgaaaaat gattttctgg
    17581 tgttccattt gatagcgagc gataattgtt cagattaaat aattgataat caaatggcgg
    17641 attatttgtc aacacaccga caggattatc atataaatga agtccatctt tcatactttc
    17701 aattactatc gatttttctt tatcagctaa taaccagtgt aaaggtgaca agggtagttt
    17761 ttcactaaaa tcgatattga ctagattgat agtagccaat aattctttag cttcgtctat
    17821 ggttgagcat tgccctagta tccatggaat aaactcaaat ggtgcaacgt tatcttttcc
    17881 ttcttgtaat tctttataat cagcattgcc aggaaaattc agtcctgcca tactcaaccc
    17941 tttttcattt gtagcctcat aatagagtgg atagttggat accccagctg ctattcctat
    18001 cattgcataa tgtttatcta aattttttac ctttctaaaa tcgaaacgat aatttcttgg
    18061 agtaatagtg accacttcat tataagacat ctcataatca aaattccttc caaaataatg
    18121 gtctttcgtt gcataagtga ttgcggtaca catcgtttcc cgtcctaaaa caaatcgttt
    18181 ttacactttt atcttacatc tagtattttt tttatcaaaa aaaggccaaa gaagattgat
    18241 ttactagcat ttataaaaaa tagtactcag ggggaatcaa aaaatattgt aggttaaaag
    18301 tacaacaata aggggctgtg acaaaagtct tgtcacagcc cctt
//
LOCUS       DACENJ010000034        18258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.034, whole genome shotgun sequence.
ACCESSION   DACENJ010000034 DACENJ010000000
VERSION     DACENJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.034"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(45..1937)
                     /locus_tag="IW748_000872"
     CDS             complement(45..1937)
                     /locus_tag="IW748_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103796.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1934..3661)
                     /locus_tag="IW748_000873"
     CDS             complement(1934..3661)
                     /locus_tag="IW748_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103797.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3677..4327)
                     /locus_tag="IW748_000874"
     CDS             complement(3677..4327)
                     /locus_tag="IW748_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1103798.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4531..4827
                     /locus_tag="IW748_000875"
     CDS             4531..4827
                     /locus_tag="IW748_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1103799.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5019..5639
                     /locus_tag="IW748_000876"
     CDS             5019..5639
                     /locus_tag="IW748_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1103800.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5731..6897)
                     /locus_tag="IW748_000877"
     CDS             complement(5731..6897)
                     /locus_tag="IW748_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1103801.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6909..7262)
                     /locus_tag="IW748_000878"
     CDS             complement(6909..7262)
                     /locus_tag="IW748_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1103802.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7390..8412
                     /locus_tag="IW748_000879"
     CDS             7390..8412
                     /locus_tag="IW748_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1103803.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8851..9285)
                     /locus_tag="IW748_000880"
     CDS             complement(8851..9285)
                     /locus_tag="IW748_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103804.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9395..11254)
                     /locus_tag="IW748_000881"
     CDS             complement(9395..11254)
                     /locus_tag="IW748_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1103805.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11372..12531)
                     /locus_tag="IW748_000882"
                     /pseudo
     CDS             complement(11372..12531)
                     /locus_tag="IW748_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(12838..14262)
                     /gene="araA"
                     /locus_tag="IW748_000883"
     CDS             complement(12838..14262)
                     /gene="araA"
                     /locus_tag="IW748_000883"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1103806.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14278..14976)
                     /locus_tag="IW748_000884"
     CDS             complement(14278..14976)
                     /locus_tag="IW748_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1103807.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14933..16594)
                     /locus_tag="IW748_000885"
     CDS             complement(14933..16594)
                     /locus_tag="IW748_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1103808.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16812..18212
                     /locus_tag="IW748_000886"
     CDS             16812..18212
                     /locus_tag="IW748_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1103809.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
BASE COUNT         5208 a         3856 c         3286 g         5908 t
ORIGIN      
        1 acttttgtca cagcccctaa aaaaaataat aaatttgtac tattctatga agcttcttct
       61 tcagaaaact gactgttata aagatctgca tagaatccgt ctttttccat caatgtatcg
      121 tggtttcctg tttctacgat cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt
      181 attgtagaca agcggtgagc aacaacgaaa cttgtccggt tttccaatag gcggttcatc
      241 gctttttgaa tcagtatttc tgttcgtgta tctacactag atgttgcttc atcaaggatc
      301 aggacatctg gatttgctaa gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc
      361 gaaatattgc ttgcatcctc gtttaataca gtgttatatc cttctggcaa tttacgaaca
      421 aaatcgtcta cgtgagcagc tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg
      481 ttcccatatt tgatattgtc atagatactt cctgtaaaca gccaagtatc ttgcagtacc
      541 attgaaaact gtgcgcgcaa ttcatcacgt gaaagatcac gggtatctac accatcgtaa
      601 cgaatactac ctccagaaac atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc
      661 cctgcaccag tcggacctac aatagcgacc atctcaccag gtttgacatc caagttaaaa
      721 tcagtcatta gcaagttatc tggtgtgtag ccgaattgaa cattttcaaa acgaactttg
      781 tatggtgtat ctttttcttc tggaaggcca gatttttcat tggacatctc ttcttcatcc
      841 aacacttcaa atacccgttc ggctgaagca actgttgatt ggatagtgtt catcaaactt
      901 gcaagttgag tgattggttg agagaactgt gttgtatatt gcaagaacgc ttgaacatcc
      961 cctaatgtca tgttcccatt tgcaactttt actccgccta gtacagcgac gaacacatag
     1021 cctagatttt taacgaaatt catcaacggc atgatgattg ccgaaataaa ttgagctttc
     1081 caaccagcgg catacagatt ctgattttct ttttcaaata cttcctggtc tttttcagta
     1141 tgattgaatg ttttaacaat ggtatgacca ccatatgttt cttctacttg gttattcaat
     1201 aaacctaaac ttttttgctg atctgcgaaa aacacttgtg atttcggcgc tacgatcatc
     1261 acgatgatca agcttaaagg gatcatcaat aacgcgacta acgtcaattg ccagctgatt
     1321 gaaagcatca tcacgatgac cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa
     1381 ttttgctgca gcgtgcttgc gatgttatcc atatcattga ttgctcgaga catgatatcc
     1441 ccatttgtat gtgtatcata ataggaaatc ggtacacgat tcattttggc ttccaaatca
     1501 tgacgtaatt cataaactgt tcgttgggaa acacgtgtca taataaattg ctgtaaaaag
     1561 ttgaatacag cagaaatcaa atacatcacg attactgtcg caatgatctg cgcgatttta
     1621 tcaaagtcta tcggaaattt tgttacttgt tggccttgtt gcatctgttg agcgccagcc
     1681 attacacctt tgaatatttc tgtggttgct ttccccaaga cctttggtgt ttggatttgg
     1741 aagatcacgg ctgctattgc tagtacaaat acagcgatga tcgcaaccat acgtttagac
     1801 atatagccaa acaatcggcg taccgttccc cagaaatttt taggcttttc accttttaca
     1861 ccgagatttc tgccaggtcc accatgtcct ggaccgcctg ctggtccatg agagggacgt
     1921 gtgtttgttt tactcatgct aagtcctcct ctcttagttg tgaatgaacg atttcttgat
     1981 aagtttcgtt tgttgctaat agttcttcat gtgttccttt gccaaccagt tttccttcat
     2041 ctagcacaag gatcatatct gcatccatta ctgtacttac tcgttgagca actaaaacgg
     2101 tgatactttc tttcatattc gttttcaatg cttggcgaag attggcatcc gttttgaaat
     2161 ccaacgctga gaaggaatca tcaaacacat agatatctgc tgttttgact aatgcccgtg
     2221 caattgccag acgctgtctt tgtccaccag agaagtttcc tcctccttgt tccacatggc
     2281 tatctaagcc atcttccatc tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt
     2341 tccaaatcat ttcatctgtt gcatccggct taccatactg catattgtct cggatcgttc
     2401 cagagaacag aactgctttt tgaggaacaa agcccattaa ttctcgcaaa ttatattgag
     2461 tcatgtcact aatgtccgtt ccatttattt ggatcgaacc agattcgatg tcatataatc
     2521 gagggatcaa attgactaag gtcgttttac ctgaaccagt cccccctatg attgcgacga
     2581 tctcgcccga cttggcttgg aaatcaatat cttccaatgc aagtttttct gctccatgat
     2641 atcgataatt gacatgattg aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt
     2701 taggattttc agggtcatgg ataccgattt tttcatccaa cacttcatta atacggtcag
     2761 ctgaagcttg tgcacgaggc accatgataa aaatagcgga aagcatcata aagctgatca
     2821 agatctgcat cgcataagtc ataaatgcaa tcagattacc gacttccaaa gtcatatctg
     2881 ctatgtagtg tccaccaaac caagtgattg ctacgtttgt accgctcatg atcaatgtca
     2941 tcattggcat catcaatgcg acgatcgtat taactttgat tgccgtattt gtataatctt
     3001 tattcgctaa atcaaaacga ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc
     3061 tgacccctgt caatccttct cgaaatacca ggtttaaacg gtcagttttc ttttgcatac
     3121 ttttgaataa tggaacagca aagtacatga ttccgccgat caaaataatc attactggga
     3181 tgacaactaa gaagattttt gtcaactgat gatctttttg ataagcaagt atgctcgcgc
     3241 caatcaacgt gattggtgca ttgatcatca tacgtaaaaa catttgtgta accatttgga
     3301 tttgagtcac atcattggtc gttcttgtga tcaaagaggc cgttccatat ttatcaaacg
     3361 catcttttgt cagactctct gattttttat agacatccgt acgcaattgc ttacccagtt
     3421 tctgtgattc acgagtagca aaaaatgtat ttgcgatagc agctaagata ctgatcaaag
     3481 aaaagccgat catcacaaaa cctgtatgcc aaatataatc gacatctcct tgcgctaccc
     3541 ctttatcaat aatattggag gtaagtgtcg gcaaatataa atctgccagt acttggatca
     3601 ccatgaaaac tgcagctgca attgctgaag tcaaggagat ccgttttacg atttttagca
     3661 tctagttccc ttctttctat ttatatactc cgtttttcag ccaagaaagt ttcaaattga
     3721 attcactgat tgcttgctct actgagtaat cttcagatgt tgaaaataag cgatacgaat
     3781 gcgcaattgt tgtaaataca gtattcatca ctaattggat cagtaattcc aattcatgat
     3841 cattttttac tttcagcttc gtccgatcga tcaattcaag caactctttt gcatcttgtt
     3901 tgtgttcttt atgtggatcg tgaggcgttt tttggcgact cgtatgagga gcgacttttc
     3961 ttgaggcacg gtaatccatg ttcataaaca agttacgata aaaagaagca ttctcgcctt
     4021 ccaaaatctc ataaatcatt ttagaaaaat aggattcgaa tcctttgaat agatcaccat
     4081 cggattcttt cattagttgt aacatatctt tagagctgtc acgtctcatt gtctcaaagt
     4141 aataaaaata aagatcctct ttatcttcaa aatactgata gaaactgcct ctagggatat
     4201 ccgctaattt gacgatatta gcaatcgaag cttcttttaa gggtactcta gaaaactcga
     4261 ttctcgcagc ctcgattagt cttttttgtt tttctttagg caaatggaaa aatgtctgtg
     4321 tcggcatatg gattctcctt tctttcaaga tgacacattg tcatatgacg ctatgtcatt
     4381 ataacacctc ctacctgttt tgcaagacat ttgcatagtt tttttcatta tcatccgata
     4441 cattaaaaaa gtgataagag gaatccttcc atttgaatta atgagagttg cccattaaaa
     4501 tagccacata ctaaacaaag gatgagataa atggcccaat caattagtgt ccaagacttc
     4561 aataaattgc ctctcgatgc tgacagcatc gttttagata caagaaatac cttagattac
     4621 acaaaaaacc acttaagcca ctcgctttct atccccgctg ctatcttgcc aagacaatta
     4681 aaaaatctag atccaaagaa actttattat attctaagct atacaggcag gcgatcagaa
     4741 gcattagccg atcaacttac tgctaatggt tttcgtgctg tttatatcat tggtgggatg
     4801 gaagctcttc agtcgagtgt tgcttgaata ttgcttgtta gattcaaaag aggctgtgac
     4861 aaaaaatcgt gtcacagcct cttttatacc ctgtcttttt ttagttattt attcttcaac
     4921 cttatcaata tgacttgata gccttttctt tttcgagtaa actgattttg ttagtcatag
     4981 ttataacaat catagcaata aaaggagaag gtaagcatat ggcatctgtt ttagtagtca
     5041 aaggtcatcc cctaactgca gaagaatcgc gcacagttaa ggcattgacg tcttttttaa
     5101 caagttataa agaaaatcat ccagatgatg aagtgactgt tttggaactt tatcgcgatg
     5161 atattccgga aatcgatgaa gaattattat caggttggga agcattacgt gctggtgcag
     5221 aattcacttc cttatcagaa agtcaacaac aaaaaattgc tcgtttcaat gaattgactg
     5281 atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc cttatggaat ctaaatatcc
     5341 caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc tggaaaaacg ttccgctata
     5401 cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc actgcacatc caatctaatg
     5461 gcggtgtata caacggacaa gactttgcct ctcaatatgt aaaaggtatt ttaaactttg
     5521 taggcatcga tcaagtagat cagctattca tcgaaggaat cgattatgac cctgaccgtg
     5581 cagatgagtt gatgcaaaat gcattagata aagctgctgc tttaggtaaa tcattttaaa
     5641 aattgagata aatgcataca aaagagacag aggaaaaaat cttcaaagca gcatttgtgc
     5701 cttggatatt ttcccatctg tctctttttg ttattctttc tgctttttca acggattttc
     5761 ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca tgagtaatca ctactttagc
     5821 tgctgcataa acaggaatcc ccaagatcac accgatgatt ccgaacaatt tcccagaagt
     5881 cagcagaact aataaaatcg ttactggatg gattgctaac tgattcccta gtactaatgg
     5941 agagatcaag cgtccttcta ttgtctgctc tactgcaaaa acgatcagta ctttgatgat
     6001 catgaatgga cctgctacaa tggctaaaaa gatagccgga atcatcgcta aaaatgatcc
     6061 taagtaaggg atcaggttta aaaaacccgc aataatcccc aacgtgacag catactctaa
     6121 accaatgacc gcaaaaccaa tcataaacat gattgccaca gcaaaagcca cagttagttg
     6181 tcctcttata taagaagaaa cttgactatt catttcactc agcacattca acgtcggttt
     6241 ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct aattgtttac cgtctttcaa
     6301 tagataaaat aagataaacg gcatcgtcag cagagccact aaaatagtcg ctaccgcgcc
     6361 gacaaaactt ccaagactct gaactgtact cttagatata tcttggatca tatcgcctgc
     6421 cgaattcatg aacttatcac ctgctgtttc caactgttcg cgaaattggt tgaacagcgg
     6481 gtcccgcaaa atctcggtta atttattatc aatcgtatct acatattgag ggaagttact
     6541 gatgaagctc actgtttgtt cctgaatctt agggatgatc actacgctcc cccatgcgat
     6601 tagtgcaaca accaaaacaa acagcccaat gatactgtaa agccttggta cttttcgctt
     6661 ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag attcctgcta aaatgatagg
     6721 cagacctacg attgctaaaa attgccagat tggtgcaaac aaatatgaaa cttttgtaaa
     6781 taaaaagacg atcagcaata ttaacaatac aacaagtaag gctgtgacta cttgattatt
     6841 taaaaaccag cgccaaaacc acgataccgg tctgttcttc ttattttttc cttccataat
     6901 tcctcctgtt agttataaaa aatttggctt cccaaaccaa tagcaggcag tctatgcgga
     6961 tgaagataga ttccatttgc aatttgtcct tcttctggac aagcatcaaa aatcaatgat
     7021 acgtgagcta ctgtctttaa attcccattg gccatcggac cgacataatc gatcgtatat
     7081 acctgatcat caaaagcaat tgttccgcct gctttcaaag aaaaaggttc gtcttttgat
     7141 acttcttgaa tcaccgaata ttttcgtaag gcagctgttg ctgttttatc gaacaaaatc
     7201 agcatgggtt cttccttgtt tattgcttct gtcccaattt ctgtaatctt tgcctgaatc
     7261 attgcactct ctcctttaat tgctttcaaa aacagtataa cacattctca ttctttagcc
     7321 gtgtggaata attatgtgat agaataaaaa cgaaaactat ttcattacaa ttaggaggaa
     7381 tcagctcata tgatcgaaaa gataatttta ggaacctata cgcgtcgcga aagtaaagga
     7441 atctatacga tcgaccttga tacagaaaaa gaagaattag ccaacctttc attagctgtt
     7501 gaagaaaata gccctaccta tgtagcaaaa agtaaagctg gcaatcttta cacagttact
     7561 agtgtggatg gattaggtgg tgccggtgcg tacgataagg attttcattt tttgaatgcg
     7621 gtaacagagt ctggcgctcc tttgtgttat gtagcggttg atgaagcacg tcaattacta
     7681 tacggggcga actatcataa aggtgaagta aatgtctacc atatattagg agatggcgaa
     7741 ctgaaagcag cagattcaat attccatcaa gaagcgacag gtcctcataa aaaccaagat
     7801 catgcgcatg tccactatac ggatctgact cctgaccaac gattagtcgt atgtgatctt
     7861 ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg gcaaattgaa tcttgtaact
     7921 accttcactg cagagccagg aacaggccct cgccatcttg tctttcatcc aaatggacaa
     7981 ttcgcttatt tatttggtga attagacagc acagtccgcg tgctttctta taataaagaa
     8041 acaggtgctt ttgaagaatt acagaaaata tcgaccttgc cagaagaatt cgacggtgaa
     8101 aatggagggg cagctatccg catttcaaat gatggcaagt tcctttatgc ttctaaccga
     8161 ggacataact cgattgctgt attcgcagtt tttgaagatg gttcccacat tcaaaacaag
     8221 caaatcatct caacagaagg ggattttccc cgcgatttcg cactagatcc aagcaataat
     8281 tttgtcgtgt gtgccaatca gaacaccgat aatctgacat tatatcgccg tcatcctgaa
     8341 acaggattac tggaattact gaaaaaagat gtctatgtac cagaatgtgt ctgtgtttat
     8401 tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc aagacgtatt agacagacta
     8461 cgggaagaac taaatattcc tttctttgat gggaaaatcg aagataaaga gtatagcgaa
     8521 gaggaatatc agaaactcaa agccgattta cagaactact ttgaacaata tgtacagaat
     8581 gtagaaaact aatcagcttt ccatactatc tgaaaagaac atcaggacaa gacttttccg
     8641 tctatcctga tgttcttttc tacaaataaa aaaccgccga gatggttaga ctcgacggaa
     8701 aaggagtatt tactcgattt tgagtaagta caaggttgtt gttggttata ggtatgtcat
     8761 tatcataaag gattttgatc actctatcat aaaggtaata ttacatttat attagataac
     8821 tctagttttt attgttatca aagatttctt ttattttttc aacaacaaat tagtcatttt
     8881 ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt tttgcttcag atgtattgaa
     8941 tagatcttca tctacccctg attcaagatc gatactttgg atcatttctt cacagcctgc
     9001 tacatatgaa agataagctt tttcaaaatt tttatgaatg cccattgctt ttgctggtgg
     9061 acgcaaagta ctgactttca taagcattgc ccgatatttt tttgttcctt cttcaaataa
     9121 caggatgatc tcttcaagag tcgatttgtc cagttcactt gtttttcctt catcaattgc
     9181 gtttctgatc acttcatatt tcggatgcat tccttctccg attgcttctg tttccttgac
     9241 gatttgatcg atggactgca cataatagcc aatattcggt aacatgttcg ttttcctact
     9301 ttctccattg ttgttattat ttattctact tgtttttatc ctcaaatgac aagtacttga
     9361 aaaaatttaa tggtttctat acagctcgcc attcttattc gtgctcaaag aaaccatctg
     9421 ctaaactata ttgtttccct gcgacaatgt catattggat caattgatat tcgtcgtaaa
     9481 tctcttgttg cgagcgattg aattgcgctt ctttatacca ctgtccgttt tgatagtaca
     9541 gttctcgttc aaacgccgga agttcctgtt ctaatgataa aagcagctga taaaagccat
     9601 ttgttgtctg attcgtctgt tccattagat tcgctgcaaa ataaaacgga ctggtgattg
     9661 catcgtgagt cgtttgcttt tctaattctc ctttactgtc gaacataaga aattgtgttt
     9721 catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc tgaatagatc cctggaagat
     9781 gatcccccca aaaaacaact aaagtccttc tagaaagatt cttcaattct tccgtgaact
     9841 gcttcagtgc cgtgctgcta taggaaatat cttgcagata attctctaag tcaagtgtcc
     9901 cacttccatc tgaagttttt gcaggatagt ctagattatc atactttcca tcgtagggca
     9961 tatgtgtctg catcgtgact aaatgaatga actgaggtgt tttttcttcc ttgagaagtg
    10021 tcattacttc tttgtaagct gactcatcag aaatatatgg attattctct atcgtatccg
    10081 tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc taatgtctga taaacatcct
    10141 ctcttttata catcgaagta ttgtatggat ggatcgctgt cgtctggtat gattgtgcgt
    10201 ttaacgctga aacgatagaa ggaagctgat ccatttttgg tacaagcatc gtgtaagggg
    10261 tggtgagttg cgcattaaat aacgccatag aaaaaccagt caatgcttca aattcgatat
    10321 ttgctgttcc accgccatag ttttgagaaa gcatattgcc actatatgtt tgatcagcta
    10381 cttcataata atctgccaat ggctcaccag atacttctac gccgtttaat cgactaggat
    10441 ctgagaaact ttcactcatc acgaacacga tgttcggcga ctcttcctct acagttttat
    10501 ttttttcatc ggctagcttc tggtattttt cagtgatttc tttgatcttt tctttcgaat
    10561 atccttcagg ttcatccatt ggttctacct tcagattgta caaaaaccca ccgataaaac
    10621 cagtgttata gtagttcatt ttctggctgt aaggtatcca tagtgccgtt ttattatatg
    10681 cttttcgcaa caaattatcg ggattgttga aattactgat ataccctagc agtccaattg
    10741 ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct tccttttttc aggcttcgaa
    10801 acatgtacca gcaaaacaat cctatcacgg ttcctgcggc agctaagatc actgtaaaca
    10861 gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt gatcatcttc aaatcgtctg
    10921 gataaatcgg ttcttgccga taactcattt tcatataatt tgcaaaacct aatacaccga
    10981 tggctacgac atacgtaagg gaacctaaag ggacagatcc tgctagtgca atcagaaaaa
    11041 taagaataat cagcaagacg agacaggcta agaagaattt ttccgtatgc caagaaaaag
    11101 caaatttcaa cgcaaggtct acggatagat cattttgaca ccactgcaaa aataaattac
    11161 taaaaacaac aagaaaaaag atactgatga ttgctgctgt tccttttaaa atctttttct
    11221 gtttggtcga aagtctgctt atatatcttt tcaaatgctt gctccttcat ttctatcaat
    11281 aaaagaatgt tgcttctctt attttagttg tacgaaattc gaaagtaaag aacggaaaga
    11341 agaattaatg aaatccaaat attctctttc tttatggcct ttctggtttc tttaataagc
    11401 ggacctttcg tactccagaa attttttcta gctgttgttt cacttgattg agctgttcaa
    11461 aatcagtact gtctatgtca atcaatatgt agacatgctc attcttatgg ttacgcgata
    11521 tatctgcgat cgctagctcg ttctcattca gcagagaaaa tatctctgct aatatattgg
    11581 ttttcttttg ataaaatata gtgaatcgaa acggcgcttg gaaaaacatt cgtgcggatg
    11641 gataattgac cgcttctcta accgtaccga acaaaagata atcttttaag gcttcaactg
    11701 ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc cctagatgag gcaacataag
    11761 tatacggtca ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg
    11821 ttgtttttcc aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt
    11881 gataagtaca gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt
    11941 atcttctgtc aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa
    12001 atctgcttgc cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat
    12061 ccctaaacta tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc
    12121 gatggttttg ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc
    12181 taaaatatta ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga
    12241 agataagagc aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt
    12301 attcatgacg atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac
    12361 accagctctt gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact
    12421 acgaataact attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa
    12481 cttttctatc tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc
    12541 tccttactaa caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc
    12601 ctagaagcaa aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac
    12661 aagttactgc cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata
    12721 aatggtgttc cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca
    12781 aatttcgtca atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta
    12841 tctaataacc actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt
    12901 tagatttagc gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc
    12961 ttcatggaaa ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc
    13021 agtcgttggt tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc
    13081 tgccatcgat acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc
    13141 ttctctatct cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt
    13201 cggatctact tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta
    13261 agtataatct tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc
    13321 aatcgctgca gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg
    13381 ttgtacagct agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa
    13441 agcgttatag cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac
    13501 ttttacatgt tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc
    13561 cttgtactct tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac
    13621 aagatctccg ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt
    13681 gtctccttct gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc
    13741 aaaactttca ttataggcta ctgcaacatc catccaatca gcaatctctt tttggatctc
    13801 aggtcgtttc cagtagccaa cgacgatttt attttgttta tttaatcgag cattgatgaa
    13861 cccatattcg cggtcaccat gagcggattg atttaaattc atgaaatcca tatcgatcgt
    13921 tttccaagga atgctttcgt tatattgcgt agctaaatga agcaatggtt tttgtagaag
    13981 ctttgtcccg cggatccaca tttttgctgg tgagaaagta tgcatccaag tgatcacacc
    14041 tgctacttct tctcgataat tgacctcttt catgattttt gtgatcgtat cagcagtaac
    14101 tgctaattct tgaaggcgga ttggataagg caattggcca ttttcattca gttcatcgac
    14161 catttcttgt gcatgtttct ttacttctcg cagtgcttct tccccatata aatgctgtga
    14221 tcctacaaca aaccaaaatt ctttttctcc aatattcaac ataattagcc actcctttta
    14281 tttgttttgt ccgtaatacg cattttttcc gtgttttcgt aaataatgtt tatctaaaat
    14341 ccgttgaggc agctcttctg aaaatgaatt gagctgtctg gtaaataaat tcatcttgca
    14401 tacttcatct agtacgactg cgttcattac cgcactttgc gcatctttcc cccaagtaaa
    14461 tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt tgcgttcttt
    14521 gaatgtttct atgataactt ttcctgtttc atattcgtac ccgctgtcga tttcctgttg
    14581 tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat
    14641 cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt gagtatggac
    14701 gattcctccg atttgaggaa atgaacgata taaaacagca tgagtaggcg tatccgaaga
    14761 gggattgagt ttaccttcaa tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc
    14821 tgtcatctct tcataactga ctccgctagg tttgataacg aaatatcgtg tttcaggatc
    14881 aaaagcgctc acatttcccc atgtatattt gacgagtccg tgtttaggca agtcaagatt
    14941 tgcttggtat acttcttctt tcagttgttc cagcattttc tatcactcct ttttcttgca
    15001 attgatgaat cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc ataaatatct
    15061 gatagcctgc tatttcttct gctgaaggtg tgatggtgat tccttggtct tgtccaaaca
    15121 cgtcttctgc caagaagttt tctaaagact tcccatgtcg atccatcata taggctgcta
    15181 ataacgcgat tccccacgct ccaccttctc cggctgtgtc catcactgta actggtgcct
    15241 ccatagcaga tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc
    15301 ccactaaacg atcaatttgg acattctcag atttcaatat atccattccg attctcattg
    15361 caccaaaagc actggctaaa tggatccgca tgaaattcgc taaatcaaaa cggctacctg
    15421 gtgtacgtac aaataatggc cttccttcat ttactcctgt aatgttttca ccggaaaaat
    15481 aaccataaga aagtaatccc ccgcaatccg tatcgccttc tagcgctttg ttgaataata
    15541 ccgtgaataa tttatctctg cttatctcca tacccaaaga attggtaaat tcttcaaaaa
    15601 tactgaccca agcattgata tccgaagagc agttattcgt atgaaccatg cctactagtt
    15661 ctcctgaagg tgtagtgacc atgtcgattt ccggatggac attttctagt tctttttcta
    15721 atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccct ttggctacac
    15781 tgttcgtagc gaccataccg gttcctgcat ccccttctgg cggacaaacc gggattcctg
    15841 gtctcaaatt tcccgtaggg tcgatcagat aagcccctga ttcgcttaac ttgccagctt
    15901 cttctcctgc tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt
    15961 tttcgattaa ctgaccaaaa atatttttca tttcttgatg gtaggttttc gttgaaggat
    16021 cgataggaaa catgcctgat gcatctccta cgcctagtac tttctcacca gtaagctgcc
    16081 aatggacata acctgcaagc gttgtcaaaa aattaatttc tttcacatgt tcttctttat
    16141 tcaaaataga ttgatacaaa tgagcgatac tccatctctg cggaatattg tattgaaatt
    16201 tttccgttaa tattcgttct gcttcttcag tgattgtatt tcgccaagta cgaaatggta
    16261 ctagtaactg ttccttgcta tcaaatgcta gataaccgtg catcattgca ctgaacccaa
    16321 tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa tacttctgct gccatttttc
    16381 ggtaactgac gcgcagtcct ttccaaatat cttctaatga ataggtccag ataccatttt
    16441 ctagtttgtt ttcccactca taactaccag cggcaatagg agtaaaagat gaatcaatca
    16501 atacagcttt gatcctagtt gatccaaact caatccctaa agatgtacga ccatctatga
    16561 catctaacat actttgcgca tttacttcct tcataatgat ttaactccct ccttctcgtc
    16621 aaacctgatc aaaccgtctt tttagtagcg cttacaatga ttattgtata cttttgtacg
    16681 tacatgtcaa gcagttattt acaagaatac aataaaaaac acttgttttg tacgtacata
    16741 agttataaaa tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg
    16801 agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat ttttttggcg
    16861 catttggcgg cattctattt ggatatgata ttggtgttat gaccggcgcc ttaccttttt
    16921 tacagcatga ttggggtttg gcaggtaaag cttctttgat aggatggatc acgtcttcgg
    16981 tcatgttagg tgcgatcctt ggcggctctt tatctggtct tctttctgat aagcttggac
    17041 gacgaaaaat gattcttctt tctgcactta tttttatggc tggttctgtc ctttctgcat
    17101 ctgcccctca caatggttct tattttctga ttgcagcaag gattcttcta ggtttagctg
    17161 ttggcgctgc atctgcccta gttcctgctt atatgtctga aatggctcct gctagactaa
    17221 ggggaagatt gtcagggatc aatcaagtga tgattgcttc tggcatgctc ctttcctatg
    17281 ttgcagatta tttgctaaaa gggttgcctg aaacgatggc ttggcgcgtg atgttagggc
    17341 tcgctgctgt ccccgcgctg attttatttt tcggtgtttt agctctacca gagtcaccgc
    17401 gttttcttat gcaatctggc cgcttggaag aggcaaaaag agtattgaac tatattcgga
    17461 caccaaaaga agctgaacag gaattcgaac aaatccagct gaatgtcaaa caagaaaaga
    17521 caaccggaac ttcttggcat actttgtttt tagaaaaata tcgttcctta gtcattgccg
    17581 gtattggtgt agcagtattc cagcaatttc aaggagctaa tgctattttt tactatattc
    17641 cgctgatcgt agaaaaagcg actgggcatg cagcgagcga tgcattgatg tggccgatca
    17701 tccaaggaat cattttagtt gctggttctt tgctcttttt ggttattgca gacaagttca
    17761 atcggcgaac tttattgaaa ataggcggta gcgtgatggg actttctttc atcctaccag
    17821 ctgttttagg aactgtactg gatgctcata caaattcgtt gttgattctc ttgtttttat
    17881 gtatttatgt cgcattttat tcttgcacat gggctccact gacatgggta atcgtaggcg
    17941 aaatattccc actagctgtc cgcgggcgag catctggcct agcctcttct tttaattgga
    18001 ttggttcttt cctagttggc ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa
    18061 tcgttttcgg catttttggg atcatctgct ttttaggggt tttatttatt caagagatcg
    18121 taccagaaac tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct aaaaaaactt
    18181 atcctaaacg tatttctatc catcattctt aaagaggttg tgacagaagt ggtcagcttc
    18241 aagaaataag aaggaacc
//
LOCUS       DACENJ010000035        18253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.035, whole genome shotgun sequence.
ACCESSION   DACENJ010000035 DACENJ010000000
VERSION     DACENJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.035"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(52..729)
                     /gene="phoU"
                     /locus_tag="IW748_000887"
     CDS             complement(52..729)
                     /gene="phoU"
                     /locus_tag="IW748_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1103810.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(742..1500)
                     /locus_tag="IW748_000888"
     CDS             complement(742..1500)
                     /locus_tag="IW748_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103811.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1516..2322)
                     /gene="pstB"
                     /locus_tag="IW748_000889"
     CDS             complement(1516..2322)
                     /gene="pstB"
                     /locus_tag="IW748_000889"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103812.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2337..3221)
                     /gene="pstA"
                     /locus_tag="IW748_000890"
     CDS             complement(2337..3221)
                     /gene="pstA"
                     /locus_tag="IW748_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1103813.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3221..4141)
                     /gene="pstC"
                     /locus_tag="IW748_000891"
     CDS             complement(3221..4141)
                     /gene="pstC"
                     /locus_tag="IW748_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1103814.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4256..5113)
                     /locus_tag="IW748_000892"
     CDS             complement(4256..5113)
                     /locus_tag="IW748_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1103815.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5450..6898)
                     /gene="cls"
                     /locus_tag="IW748_000893"
     CDS             complement(5450..6898)
                     /gene="cls"
                     /locus_tag="IW748_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1103816.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNIGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(7050..7943)
                     /locus_tag="IW748_000894"
     CDS             complement(7050..7943)
                     /locus_tag="IW748_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103817.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(7927..8613)
                     /gene="ftsE"
                     /locus_tag="IW748_000895"
     CDS             complement(7927..8613)
                     /gene="ftsE"
                     /locus_tag="IW748_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1103818.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(8718..9819)
                     /gene="prfB"
                     /locus_tag="IW748_000896"
     CDS             complement(join(8718..9755,9757..9819))
                     /gene="prfB"
                     /locus_tag="IW748_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1103819.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(9945..12479)
                     /gene="secA"
                     /locus_tag="IW748_000897"
     CDS             complement(9945..12479)
                     /gene="secA"
                     /locus_tag="IW748_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1103820.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(12701..13252)
                     /gene="raiA"
                     /locus_tag="IW748_000898"
     CDS             complement(12701..13252)
                     /gene="raiA"
                     /locus_tag="IW748_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1103821.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(13380..14099)
                     /locus_tag="IW748_000899"
     CDS             complement(13380..14099)
                     /locus_tag="IW748_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1103822.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            complement(14096..15409)
                     /locus_tag="IW748_000900"
     CDS             complement(14096..15409)
                     /locus_tag="IW748_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1103823.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            complement(15490..15810)
                     /locus_tag="IW748_000901"
     CDS             complement(15490..15810)
                     /locus_tag="IW748_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1103824.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(15918..16205)
                     /locus_tag="IW748_000902"
     CDS             complement(15918..16205)
                     /locus_tag="IW748_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103825.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            16331..16975
                     /locus_tag="IW748_000903"
     CDS             16331..16975
                     /locus_tag="IW748_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1103826.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            complement(17034..17741)
                     /locus_tag="IW748_000904"
     CDS             complement(17034..17741)
                     /locus_tag="IW748_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1103827.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
BASE COUNT         5102 a         3988 c         3183 g         5980 t
ORIGIN      
        1 ctgatttcac aacctctttt cctaaacaaa cggcagaatc accgctttag tttagaaaat
       61 ctcattgtgg ttcgtattta actcagtgat tttccctgta gccaagtaga cgatccattc
      121 acagatgttt gtcacatagt ctccgatccg ttctagataa ctggcaacat tcaagtagtc
      181 tgttccactg atgactgtct caggattttc ctgcatattt ttgattgttg cattatagat
      241 tcgattgaaa tagtcattga ctctttgatc catattggcg atcgtacgtg cgtctttttc
      301 gtctgttttg acataagcaa ctaggacgtt gtccaccatt tttttcacat aatcggacat
      361 atcggagatc tctttttcga tttccggtat tctcgtctgc ccttttacgc gaatcgtgga
      421 tttagcaaca gacaccgcat gatcggccat acgttctaaa tccgagctag ctttcatgac
      481 cgtaatgatc atacgcaaat cagttgtaac tggctgttgc aatgcgatca tttcaaaact
      541 tttcttctcc aagcgtgttt ccatgttatt gatatttata tcgttttcga taacttcttg
      601 ggctaagatt ttatcgtgtt tgatgtatgc acgaactgac ttgtgcacgg cgctgctcac
      661 catcatcccc atttcgtaga actgattatg gagattcagc aattcttctt caaattgtgt
      721 acgtagcatt ccgtttcctc cttatccaaa tcgacctgaa atataatctt ctgtctcttg
      781 tttttctgga ttcatgaata tcttcttcgt atcattgtat tcgatcaagt gaccatctaa
      841 aaagaatgcc gtcttatctg aaatacgtga agcttgagac atattatggg tcacgatgat
      901 catcgtatac cgctctctta attctaacag catactttcg attttcccag tagaaacagg
      961 atcaagcgca cttgttggtt catccaataa gatgatttcc ggattaactg ccaaaacacg
     1021 tgcgatacat acacgctgct gctgcccacc ggataaagat aaagcacttt tatgcaattt
     1081 gtctttcaca tcatcccaaa cagacgctgc cttcaggctt tcttcaacta cttgatcaag
     1141 gactttttta tctttttccc cttttaattt caatccatag atgacatttt catagataga
     1201 aaaaggaaat gggtttggct gctggaatac catgccgatt tcttttctta gttcaacaac
     1261 atctgttttt ggaccataga tgtctttgtc tttatataaa acactcccgg tgatcgttac
     1321 attcggaatc aggtcattca tccggttcaa agaacgcaga taagtggatt tcccgcaacc
     1381 agaaggaccg atcatcgcag tgatttcccc ttgattgaaa cttagatcga tcccttttag
     1441 tgcttctttc tttccataat acagatgaag atctttagat gaaataattt cttttgtcat
     1501 gttgaggcct ccgtcttaac caaaatgacc ggacacataa tcttcggttg cctgaatttt
     1561 tggtctggta aatatcttgt tagttttgtc atattccatt acttttccta gatagaaaaa
     1621 ggcagtatag tcgctgatac gagccgcttg ttgcatgtta tgcgtcacga tgatgatcgt
     1681 atagtcttct ttcaagttta caagtgtttc ttcaaccgtt cctgtcgaaa tagggtccaa
     1741 agcacttgcc ggctcgtcta gcagcaagat atcaggtttc atcgcgatcg cacgagcaat
     1801 acataaacgt tgctgttgcc cgccagataa agccaaggca cttttgtcca agctgtcttt
     1861 tacttggtcc cacaaggcag cttgtttcaa gcttgtttca acgatttcat ctaatttctt
     1921 tttgtttttt tcgccgtgct gtttcaaagc aaatgtaata ttgtcataaa tcgacttgct
     1981 gaacggatta ggacgttgaa aaaccatgcc aatccgtttg cgcatctcat aaacatccac
     2041 ttgaggcgca ttaatatcta cgccttgata catgattttc ccagtgattt tcgtattagc
     2101 gatcccatca ttcattcgat tcaacgaacg aagatacgta gattttccgc agccggaagg
     2161 accaatcagt gcagtgatct tatttttttc aaattccaga tccactcctt tgattgcttc
     2221 attgtcgcca taccagacat gtagatcatc cgtatatagt gctacttctt tctcttttgg
     2281 catggtgatg acatttgttt cattccagtt atattttttc attttgcttc ttctccttag
     2341 gctgaagtca tttttcggta taatcgtttg ccaatcgcac gagcgccgaa gttgaacagt
     2401 aaaacagcaa tgatcaacac ggcagaagcg ccagccgaaa cagaaacacc atcaggcatc
     2461 gtgccttctg tattgatctt ccaaatatgg acagctaatg tttcagcttg acggaaaatg
     2521 ctgatcgggc ttgaaacact taatggattc cagttcgtaa aatctaacgc aggtgcactt
     2581 tgtccagccg tatagatcaa tgcagctgct tcaccaaaaa ttcttccaga acttaggatc
     2641 acaccagtca aaatccctgg tgtcgcttct ggaaccacca cttttatcac tgtttcccag
     2701 cgtgacaaac ctaaagccaa tcctgcttct cgttgtgtgt aatggaccgc ttgtaaagac
     2761 tcctcaactg ttctagtcaa aagaggcaag ttgaatacag tcaaagccaa tgcaccagaa
     2821 ataatcgaga aaccatactg gaattgaata acgaaaacca agaaaccgaa taaaccgaca
     2881 acgacagaag gcaatgagct caagatttca atcgacgtgc gaatcacatc tgtcagccag
     2941 ttttttccag catattctga aagatagata cccgcaccta aagaaatagg aaaactgatg
     3001 atcatcgtga tcaaaagcaa ataaatcgag ttaaatagct ggatcccgat tccaccgcct
     3061 tcttggtagg ctttggaagg ttcggtcaag aaggaccatg aaatgtgcgg tattccccga
     3121 actaagatat aaagtagtaa agctgctaaa atcaatacga tgatccccga aactacatat
     3181 aaaacacctg tagcaatttt atctgagcgt tttgcgttca ttatttcagc tctcctttct
     3241 tcccaatcat acggatcacg atattaaaga ataatgacat caatagcaag atcagcgcta
     3301 atgaccataa cacattattt tcaactgttc ccatgattgt atttccgatt cccattgtta
     3361 agatggacgt caatgtagaa gcaggagtag taagtcctgt tggcatcaat gctgcattac
     3421 cgatgaccat ttggatagct aaggcttcac caaacgcacg agccatacca aaaacgactg
     3481 ctgttaaaat cccgggcacg gctgctcgta gcacgacttt gtagattgtt tgccatcttg
     3541 tggcaccaag cgctaatgaa gcttcccgat agtgccgtgg tactgccttt aatgcatcaa
     3601 cagtcatcgt tgtgacagtc ggcaagatca taacgaacaa cacaaatgta ccagctaaga
     3661 taccaaaccc tgtcccgcca aaaatacttc tgacaaaagg cacgataact gataaaccga
     3721 taaaaccata aacgacagaa ggaataccta caagcagctc gatgaccggc tgtaagatct
     3781 tcgttccgtt tttaggagag atctccgtca tgaatactgc tgcaccaatg gcaaatggtg
     3841 tggcaacaat agcagataaa aaggtcacaa taaacgaacc tgtgatcata ggcaatgccc
     3901 ctaccattgg tttcccattt gctccgattt ggcttggatt ccaatctgta ccaaacagaa
     3961 aatcaaatac attgatctta tcagtaaaaa aggtggccaa tcctttacta gctacaaaat
     4021 aaaaaataga taatacaacc aacacgatca gcgcgataca taaaaaactg atgaattttc
     4081 cacgctgttc catttttgaa cgttttgatt ttgttaataa tacttttttc acatcttcca
     4141 cagatgactc ccccttaatc aggtttaaca tactcactca gtgtacaaat tgaacatcaa
     4201 tttggtttta ataacatgta aaagtttagc taagtttgta aattttgtgt aaagattact
     4261 ctttaacgat atttccttgc caatcacgtt cgactttcat atgagaaaca gggatataac
     4321 ctagttctcc aacgatgtta tcttgtactt catcagataa gatatagtct aaaaattctt
     4381 ttgtcagttt ttttggatga tctcttgtgt acatatgctc atatgcccaa atcgtccatt
     4441 tgttcgttgt cacattttca tctgtcggtt ctacaccgtt aacattcaac gtatctacat
     4501 catcggaaac ataagaaaaa gcaacatagc tgattgcacc gggtgtgtcg gcaacgatct
     4561 ggcgcaccat tccgcttgaa tcttgttctt gtgcctgaac agctgtttct ccgtccaata
     4621 cccatttttc aaacgtgcta cgggttccgc ttcctgaagc acggttcaat ataactactg
     4681 gctgatcttt tccaccgact tcactccagt ttttgatctc gcctgtaaat agttttttca
     4741 ggttttccat actgatgtct tttacgccaa tatccttgtt cacaatagga gtgatcccaa
     4801 caactgctac tcgatgatcg actaatgctg aggcatcgat ccccgatttt tcttccgcaa
     4861 acaaatcaga gtttccgatg tcgactgctc ctgactgtac ttgagacaaa ccagttccgc
     4921 ttccgccccc ttgtacattg atgaattttc caggattttc tgtctggtaa gtttcagcaa
     4981 ctgtttctac taaaggctgt aaagcagaag agccaacagc cgtaatggat tctcctcgat
     5041 caatccactt tgaacagcct gataaaagaa tagctaatcc tattattggt aagaataatt
     5101 taagtttttt cattagaaat ctcctttaat cctaaagtat gtttatttta tcattttcat
     5161 tacttgatga aaacgaatca aaatcattaa ataacaaaat aaatcctcat aattgtaaaa
     5221 aaaactatct cattaaaagt ttctatgcta tactggcttt gaaatgtgtc tcttttaata
     5281 gcatatatta gcttatttct tgctttcccc aaaaaacaag agatacccca aagttaattt
     5341 cgctcatgcg agacgctttc tccatttctg acgtactctc agaaatggcc ccgttagaaa
     5401 aggacacgag atctccatct gttgatggtt ctcatgtcct ttttgtgttt taaaggagcg
     5461 gcgaaatcaa tcttgctaat ccttctttca tcttgatgat tattcctcgg ttttcataca
     5521 tttcttttgt gagatgcgta gaaattttcg agtcttcaaa aaatgctttt cttacacgtg
     5581 aagccattcg ttcatcataa atcagtgtat tgacctcaaa gtccaagcga aatgaacgaa
     5641 catcgatatt tgctgagcca actgaaacaa tgccaccatc gataatcatc gtctttgcat
     5701 gaataaatcc attctcatat gtttcgatcc ttgctccata ctcgatcagc tctgcagcga
     5761 aagaatacgt tgcccagtaa acgagaagat gatctggttt gttaggtatt tggatatgaa
     5821 cttttacgcc tgaaagaaga gccaacttca gtgcttcatg aatcgatcca tccggtatgt
     5881 aataaggcgt ttggatcaat atctcccgtt tggctaacga aatcattttc aaataagtca
     5941 ttttgatttg ttcatgttcg gtatctggtc cgcttgtcac gatttgaaca gctatatcac
     6001 cagtagagtg aatggacgga aaaaatttgg gagaatagcc tacttctttt gtatgctgag
     6061 aattccagtc catcaaaaaa cggttttgca agatatacac cgcttctccg taaatacgca
     6121 aatgattgtc tcgccaataa ccaaatttct ttaccaatcc tagatattca tttccaatat
     6181 tgaacccacc tgtatatccg attttcccat caataactac gattttacga tgattgcgat
     6241 aattgatccg aggattaata tacggaacaa atagaggaaa gaaaaatgcg atttctccgc
     6301 ctgctttttt caaattctga aaaaagctgg aagaaacttg tgtggaaccc catgcatcaa
     6361 gaaggactct gacttttacc cctcgtcttg ccgcctctgt caatgcatct cgaacttctt
     6421 cacctaatgc atcactgcga taaatataat actggaagtg gatatggtct tctgcttgct
     6481 tgatatcttc aatcagcgca tcgaattttt cacgaccatc tgtaaataaa gtgatttcat
     6541 ttttagtcgt atagagcgta ctttcaaata ccgtgagcat atagatcaat tgtttcacat
     6601 ctacttgtcc agtaggcgga tgaggataaa gccctcgcat caatgcctgt ttgtcttctt
     6661 caatttccag gttcttaccg attttccctt ggatcttcaa atcaaagatt ttctctttgg
     6721 aaatccctct tcctaaaaaa atatataaaa taaatcctac aactggaatg aacatcaaaa
     6781 cgagcagcca tgcccatgtt tgagcagttt cctttctttc tcgaaaaacg atgataaagg
     6841 aaagcaaaat attcataatc aaaaggatcg taaaaaaatt gtcaacgata attttcacgg
     6901 cttcacctct tcctctacca acttaagaat acccaagttg ttttttaaag taaagggaaa
     6961 aaagccgctt ccttctattc tcctagccaa taaaaaaaga aagctggaac gaaactgtcc
     7021 aactttctca ttttatttat attgtggtat tagaatttca agaaacgtct catagaaatg
     7081 atcgagccaa gtgaaccaat gatcacacca atgccgacca ttaaaagatt gattttccaa
     7141 acgatatttt gtggatgaat caatgaataa tgggaacgta ataatgatgg attgatcaat
     7201 tggaaaaacc atgcataacc aaatgtgatg atcaaaatcg gaagaatcgc accgatcaga
     7261 ccgatccagc caccttcaag gaaaaacggc cagcgaatat atccattttt tgcaccgact
     7321 aaacgcatga tttgaatttc acgctgtcta gacaaaatcg tgatccgaat cgtgttcgaa
     7381 atgagaaaca tcgctacgaa cagcagcaat gccgctgcaa ctaatcccca tgtccgaact
     7441 gcaccagcga tttggaaaat ccgatctgag gaagctccac cgtaatcaga acgagaaaca
     7501 ttcttcaaat ctgctgcttc ttctgctact tgttttgtat aagaaggatc agtagcactt
     7561 actacataca catcatataa cgggttattg tcaccttcaa acaatttcca ggaatcaccc
     7621 atttcgtttt ggattttttt caattcctgt tctttacttg aaaattcaac ttttttaaca
     7681 tgatctaagt cattcaattc tttttctagc tttttcatct cttcgtcttt tgtaccgata
     7741 tctacgaata cagatacatc aacgtttcct tctatatcct gagctaattt agttgcattc
     7801 atgataactg ccatgaatac accgactaaa gtcaacgtga tcgttaccgc actgactgaa
     7861 gcaactgtca tccagccgtt acgtttcaaa ctcttgatac tttctaataa atggcggaaa
     7921 aaggttctaa tcatcgtatc catattcccc ttccgcttga tctcgaataa tgcggccgtt
     7981 ttcgatagca attactcggt gccgaatcgt atttacgatc gtactgttat gtgtagccat
     8041 cacgacagta gttccttggg cattgatccg atctaataat ttcatgattt cccatgagtt
     8101 ttctggatcc agatttcctg tcggttcatc cgcgataagt actttaggcg tattcacgat
     8161 tgcccgagca atagagacac gttgctgttc cccaccggat aattcgctag gaaagactcg
     8221 aactttgtgt ttcaaaccaa caaggtccag cacttccatt actcgttttt tgatatctcg
     8281 aggttttctg ccgataactt gcatagcgta cgcaacattt tcatatactg tttttcttgg
     8341 taataattta tagtcttgga acaccacgcc gatttctctg cgtagataag gaacttcgcg
     8401 gtttttgatt gctattagat catgacctgc gacgttcaaa gttccttttg aagctttttc
     8461 ttctcgatac atcaatttga tgaatgtaga tttaccggct ccagacggac caaccacata
     8521 gacaaactct ccttgttcga tgacaatcga gagattgcgg atagcggtcg taccatttga
     8581 gtacttcttc attacatcct tcatttcaat catggcgttc tctccatatc tttttcagtc
     8641 tttagctatt atagcatggc aatgtttcaa catgcttgca aaatcttaca attttatttc
     8701 aatttaacaa tgggttctta ttgtagatgc atttttaaat agccgtcaat aaataaatca
     8761 aggtcaccgt ccataactgc ttggacattt cctgtctcta cgtttgtccg atggtctttt
     8821 accatagaat agggatggaa aacataagaa cggatctgtg aaccccagcc gatttctttt
     8881 tgttctcctc gaagtgcagc tgcttcttgt tctttctttt ccacttctaa ttgataaagc
     8941 ttagctttta acatccccat cgcttgctca cggtttttca attgtgagcg ttgtgcctga
     9001 ctagctacta ctgttcctgt aggcaagtga gtgatccgta cagcagattc tgttttattg
     9061 atgtgctgac cacctgcacc acttgctcgg tacgtatcga ttttcaaatc atctggattg
     9121 atttctatat cgatcgtttc atcaagctct ggcatcacat ctaccgaaca aaaagaagta
     9181 tgacgtcgtt tcgctgagtc aaaaggcgaa atccgtacaa ggcgatgaac acctttttct
     9241 gattttagat aaccaaaagc gttatgtcct ttaatcagca gggtcacact cttgatccct
     9301 gcttcatcac ctgcttgata atctagtgtt tctacttgat agccatgctg ctctgcccaa
     9361 cgtgtataca tacgtaaaag catgctgccc cagtcttggg actccgttcc gccagcccct
     9421 ggatgcaatt cgacgatcgc attgtttcga tcataaggtc cgtctaacag catagataat
     9481 tcatacgtct tcattttttc atctaatgca ttgatacgtg tttctgcttc ttcttgcatt
     9541 gctgcatcag gttcttcttg cagcatctcc accatcaatt cgatttcttc tagttcttcg
     9601 gcaagagaat ggaattggtc ataggtttct ttattagcgt tattttcact gatcactttt
     9661 tgtgcggctt cgctgtcatc ccaaaaacca ggttcagcca tttttgcttc tgcttccgcg
     9721 atgtcttctt ccagctgatc taagtcaaag agacctcctg aaacttgtga ccttttcctg
     9781 catttctgtc aggagatttc gaatttcgga tatttccatg ttttttcctt ctttcactat
     9841 acttttttca ttatatctag tctaacagaa tcagcaaata acagaaagaa ggtgagacga
     9901 attcttaaaa aaagaatttg tctcaccaat cctgctgtca gaaattagtt tgcatttttt
     9961 ccatgacagt tcttatattt tttcccgctt ccacaaggac aaggatcatt tcttccgacc
    10021 ttgttcacat ggattggctg ttttttcgcg ctagtattac tattttcttt ggcatctccg
    10081 tcttctgagt gagaagctgt tccttgagaa acctgttcac gctgtacgtt ttgacggatc
    10141 tcagctttca taaagattcg agtgacttca tactcgatgg cgcctaccat atcttcaaac
    10201 atcttatagc cttctgtttg atattcgact aatggattgt tctgtccata agcacgtaag
    10261 ccgattgact gacgtaattg atccattgcg tcaatatggt ctgtccattt agagtccaca
    10321 acacgcaaga tcacgacttt ttcaaattcc aacagttgct ctggaccatt tagttgagcc
    10381 gctttggtat taaatacttc ttgtgctcgt tcgtttagat actctttgat ctcctgagct
    10441 gtttttcctt cgatatctgc aagagtaata ctatcttcat ggacaagagt actgccggca
    10501 aaatcgatga tactttccaa attccagttt tctttctcta attgtgtatg cgcatctact
    10561 acacgactga ttgtccgttt gaccatgttc attagtggct tggataagct tttttcttcc
    10621 atgattactt cttgtcgctg tttatagatt acttcacgtt gttcacgcat cacatcatcg
    10681 tattgtaaga cgtttttacg tgtgtcatag ttatttcctt ctacacgttt ttgtgcagat
    10741 tctacttgtt tcgtcagcat cttgctttgg ataacagcat cttcctcacc gatcttcatc
    10801 cgatctaaga aagctttgat tcgttctgaa ccgaatcgtt tcatcaaatc atcttccaat
    10861 gacaagtaga actgtgacat accagggtct ccttgacgtc ctgcacgtcc tcgaagctgg
    10921 ttatcgatac gacgagattc atgtcgttct gtcccgataa ctgctagacc accaagttct
    10981 cttaccccaa gtcctaactt gatatctgtt ccccgaccag ccatgttggt agcaatcgtc
    11041 acagcacctt tttgaccagc attcatgatg atctctgctt ctttaaagtg atttttcgca
    11101 ttcagcactt catgaggaat acgttcttga ttcagcatat ctgataataa ctcagatgtt
    11161 tcaaccgcaa cagttccgac taacacaggt tgacctttgc ggtagcgatc tttgatatct
    11221 tgcacgacag cgtggaactt gctttgtaat gttgggtaaa gcaaatctgg acgatcatca
    11281 cggataaccg gacgatttgt cggaatctgg tatacttgga tgttataaat ttctctgaat
    11341 tcttcttctt ctgttttagc tgtaccagtc attcccgcaa gtttcttgta catacggaaa
    11401 tagttctgga aagtgatcgt cgccatggtt ttcgtttcgt cttcgatctc tacgccttct
    11461 ttagcttcga ttgcttggtg aagtccatct gaataacggc gtccatccat gatccgacca
    11521 gtgaactggt caacaatcag tacttttccg tcttggacaa cataatcgat atctaaaagc
    11581 atgatatagt tggctctcaa tgcttgatcc aagtgatggg tcaatgcagt attttcaatg
    11641 tcgtataagt tatccaaacc gaatgtttct tctgcttttt cgatccctgc ttcggttaaa
    11701 ctgattgttt ttgattgtac atcgatttta taatcttctt cttctttcaa acgtttcacg
    11761 aagttgtctg ctcttgtata taaagctgtg gatttttctg cttgccctga aatgatcagc
    11821 ggcgttctcg cttcatcaat caagattgag tcaacttcat cgacaatggc ataattcagc
    11881 ggacgttgta ccatttgatg gcggtaaacg accatattgt ctctcaagta gtcaaatcct
    11941 aattcattat tcgtactata agtgatatca caattataag cctcgcgttt ttcatccgaa
    12001 gattttgagt tgatattcaa ccccactgtt aatcctaaga aattataaag ttcgcccatt
    12061 tccgtcgagt cacgggttgc taaatattca ttgacggtaa ctacgtgtac cccttcgcca
    12121 gtcagagcat ttagatatac tggcatcgtt gcggtcaagg ttttaccttc accagtacgc
    12181 atttcaggga tatttccatc gtgaagcacg attccaccca tcaattgtac gtggtatgga
    12241 tataaaccta atacacgttt tgctgcttct cggacgactg caaaagcttc cggaagtaat
    12301 tgatctaatg tttctccttt ttggtatctg cccttgaatt cagcagtttt tgcctgtaat
    12361 tgttcatcag ttaatgctgc catttggtca gcgtatgctt caactttttt tgcaatgcta
    12421 tctaaacgtt ttagttcttt cttatcgttt tcgataagtg aacgaatcaa attggccatg
    12481 cgttgtgtaa tctccttttc cttctgtgta ttatccagac tatgtagact ttacttttca
    12541 ttttatcatt agttatagat aattgaaata tcttaagcgt attaaagtaa gaacttcata
    12601 atttattcat atttcatcta aaagaggagg aaactcaaaa aaacttgcgg cagctgtcag
    12661 tagctgctgc aagttttcgt tgttatttaa gtaaggctga tcagtctgtt tcaatcagtc
    12721 cgtattttcc atctttacgg cggtaaacga tacttgtacc atttgtttct gcatcttcaa
    12781 aaataaagaa gttatgtccc agcatgttca tttgcaagac tgcttcttca ctatccattg
    12841 gtttcaatga aaggcgtttc gtacgtacga tatctaattc agaatcattt tcgttttcgt
    12901 cctcttcatt gaataaagca gcagcatttg cagtattcat gcccgtttca cgggatttac
    12961 gattaatttt tgttttgaat ttgcggattt ggcgttccaa tttgtcaaca accaaatcga
    13021 tacttgcgta taaatctgga gaagtttctt cggcacgtaa tacaagataa ggtaatggga
    13081 tcgttacttc cacttttgct gttttttctg tataaacttt taagttcaca tgcgccgttg
    13141 catcgggtgc gtcactgaaa tagcgttcta gtttgcctac ttttttctca acgtagtcgc
    13201 gaatcgcttc agttacttcg atattttcgc cgcgtacatt atatctaaac ataaaaatta
    13261 cccctttcgt ctaccacaat caagaaggag aaggaccact ctttccctct tcttaattct
    13321 attatatcga actctaggtt atttgcaaat taaatcgcta tcaaatttcc acatctaatt
    13381 catctcgcca aagaaatagt tcggatgatt ttcggctgtg cgatctgcaa acaatctgct
    13441 gcgtggaaaa gagtctgtcc tgttgtatag acatcatcaa taatcaatat ttttttattt
    13501 tgtatcttag aaaaggtgac tgcaagttca aaaggttgag gcatctccaa tctttccttt
    13561 cgcgtgtttt ttgcttgagc cagcagatgc ttctctcttt tgagtagcat ctgaaaagga
    13621 attcttgctg ctttcaacat ctcgcttact tgattgaatc ctctttctgt tcgccttttt
    13681 tcagataaag gtattggaca atagatgtat gtcgggtatt ctttttttat ctgcgtccat
    13741 ttatatgcaa aggtgcctgc tagtcggata tctcccataa acttgtattg attgagccag
    13801 gccttcatat tttcatcgta cacaaaaaag gcacggtgct gaaaatcata ggtcggatat
    13861 ttcttcttcc attcttgaca gtcactgcag taaacgggga tagaatttac tttctttttc
    13921 gtactctcta gattgaaatg gattggtttt tgacagccag aacagcctct tgtttccaat
    13981 aaatgaaatt gtgcgcgaca aactgaacaa gtctgaggta atctgtattt cgaaacacca
    14041 ataatctcgc tgaatgttaa attttggacg atatctttac tgcagcaact gcacctcaca
    14101 gtatcttctt cccttcttca ttcatttctt tgatttcctg acaagctttt cgaatagctg
    14161 cagttttttc ttcatagaaa aaatatactt gactgttcgt ataaggccct ttacgatcgg
    14221 ctcttcctgc gatttgtacc aaagaagatt tagtaaaaac cggatgatcc gctcctaaaa
    14281 cgataacgga tattctttca aatgtgactc cacgttcaag gatcatcgtg gttaacagta
    14341 tgtcatattt tgcttctcgc attttctgga ctttttccgc ccgctgattg tctcttgaag
    14401 aaacatctgt gatctcatat ctcggaagag cctcttgaag tgtggctttc aaacgatgca
    14461 tcatcgtgat acttggacaa aataaaagga tatggttttc tttaattaat tgatgaatga
    14521 tccgcaccaa agattttatc ttcttcttgt tcttgcattt ttgtcgccaa ttattcatga
    14581 aaaccaagac gggttcagga agcaaacgcc gatgaaagcg taaagcaagt ttgtggatgt
    14641 caaacgtctg cttgatctcc tttagtaact ttttatcagg tgtggcactt aagtagatga
    14701 atcgtccgtc tggtttaatc gctgtttgca cagcatgagc taatcctgca tccccgttat
    14761 aaggaaacgc atcgatctca tcgatcacta ttaaatcaaa ggaccgataa aaacggtata
    14821 gctgatgaat cgtacagata acaaaagaag taaaggtata agccgttgct gtattcccat
    14881 ggagcaacgt gattttttct tctggaaaaa cataacgata gcgtaaaaac aattcattac
    14941 aaacatcgac tcgcggtgta caaatcgcta ctcttcctcc agcttttaaa gtttccacta
    15001 aaataggata aagcatctct gttttccctg cacctgttac agcccaaata agatgatgtc
    15061 ttttttctga atggcaaagt attctctttg agatttccgt ttgtttttca gtaagctgtc
    15121 ctgtccaaaa aaagacgact tctcgaggtt ttgcttcagg ctgatcaaac aaatataaat
    15181 cttgttgagt gtcgcatcgc cctaaatata gacaatgtgt acaaaaataa ccgtaatctg
    15241 caagctgtcc atcaatgacg gacgttctca ttccacagcg atgacaaaga atccagttgc
    15301 tcaaacgttc cactgctgcg atcttctcat aatttggtaa atttacctcc tcacagtcta
    15361 ttgctttaat atctctcgtc agaagccttc taccccaaaa atgattcata ttattcgccc
    15421 taaaaaaata tacgaaaaaa caagcgatct attttttgga tcgcttgttc gatagttata
    15481 ctgttctatt tattcaccta tgagcgtgat tgcttgatct aacacttttt cgccattcat
    15541 cataccataa tcagccatat tgatgacatc taaaggaatg ccttttggtt ccagacgttt
    15601 ctcaaaatca gctttcataa agcgaacttg tgggcctaat aacaacacat ccacgtcttt
    15661 atttgcaaga ttgttatctg cgtcagaagc ggacacagcg aaaatatcag agtcgattcc
    15721 ttttgcttca gcagcttttt gcatttttgt tactagtaaa cttgtgctca taccagccga
    15781 acatacaagc atgattgttt ttttagccat acttttctct cctttttaaa acaaactacg
    15841 caaacgtttt cttcgcgtct gttactctat tatataaaaa gaaagaaatt tgtcaacgtt
    15901 ttctaaacgc ggtttattta atcaaatagc caactagatt tcactaattt attgaacacg
    15961 acatattctt tgtccaaatt gatgacaact acttcatagc ctgcatttat ccagccatag
    16021 gcaccttttt cagggtgttt ataatcattt gcccaccaat ctgtactttc ccttgctgtt
    16081 ttgggaagtc cgttactggg aaacagcagt tcgtcaaatt gggtaaaggt caacgttact
    16141 tgagagccgc cattgttctt caaataatgc gtaattgcat cgtacttttt cattcgttcc
    16201 tgcatcccca gatccacctg cctttgtctt tattatcgca tactacagca attgcgcaac
    16261 cgttacagtg ttgaaacaac ctagaacttt atttatactg aataaagaaa atgaaaaaag
    16321 aggtgacaag atgatcgagc gctatcgtac aataaaagaa aatggacaaa gtgagatcga
    16381 gatcaaaaaa tcacgcttta tctgttctct tagacgaata gaatcagagg acgaagcaaa
    16441 aagtttcatt caagaaataa aaaaagaaca ttggaaagcg aaccataact gcagcgcctt
    16501 catcattgga gaaaaaaatg aaatccagcg aagcagcgat gatggagaac caagtggaac
    16561 ggcgggcata ccgatgttgg aagtcctcaa aaaaaatcaa ctgatcaatg tagctgcagt
    16621 agtcactcga tattttggag gaacgaaact cggcacaggc ggattaatac gagcatacac
    16681 tcatgcggtc tctcaagcat tgtctgacat tggaatcgta gaaggtacct tgcaacagga
    16741 gatctctgtg atgatcacgt atccgcaact aggaaaatta cagaattatc ttgaactcca
    16801 acattttttt ataaaagaaa tccaatatac tgatcaagtt agcgtcatct gtgctgtaga
    16861 tgaaaaaagt gctgaccact ttaaagaatc catcattgag ctgctgaacg gacaagtcca
    16921 atttgaagag ggtcctgttt cttatcacga gcaactggta actgatcata tataaaaaca
    16981 ggtgattctg attctctttt atttctaata cggaatcagg gatcacctgt tttttatttt
    17041 tccaaataaa actcaaaacg atttcctacg tattgcgtac gcacatactc aaacggcgta
    17101 ccgtcttcaa aataagaaat ctggcgcaaa cgaagaactg catctcctcg ttttacctct
    17161 aaatagtcag caattttctc agaagccaac acagcagtaa tcgtttgatt cgctggaccg
    17221 atctttttcc cgcctttttc ttctagcgtg cgatacaacg attcagtaat ctctgctttg
    17281 ctgtagccat cgatcagctt ttgtggcaca ctggcaactt caaaacaaat agggacatca
    17341 tctgcgaaac gaatacgttc cattcgcaag atcgtgtcat ctttactcaa gtttagtttt
    17401 tccatttcac ttgagcttgg cgaagtcaag aaataagaga ttgtccgact agacggtact
    17461 cgattttgtg attccataat ttctgtaaaa cttgttgcac cagacatttt ttcttgtact
    17521 ttttctcgtg caacatatgt acccgaaccg atctttcttt ctaagattcc ttcatctgcc
    17581 aagttttgaa ttgcttgtct caacgtcata cggctgacat tgaatttcac tgctaattct
    17641 ctttctgaag gaagacggtc tccgactttc catatacctt gttcgatttc attttttaat
    17701 tggtcatgaa tctgtatata tacaggtaat tgattcgaca taacgtacac ccgtcctttc
    17761 gtttcttcta ttataactgg tatatacctg attcacaact gaaaaagata aaggtttcta
    17821 tccatcaaca gaggatttag acacacatcg ttcttcctac tcctattgtc agaaacaatt
    17881 ttttagatat gtccaaccta gaaaataaca aaagacccta aaatcgatga aaatgccgac
    17941 ttatggatct tttgttatta ttagcggttt ttagccatcc atttttttgt atgattcgtt
    18001 gggcgttttg attttacccg gaccgctttt ccgccttttt tcggaatatt cacttcaata
    18061 ttgtattttt ctgatttgac caaatcatat aacttttcag ctgcttcgtc tactttttct
    18121 ttccatttca tgtggatccc tccaattctt gtagttaact taactctatc ttaaatcagc
    18181 agaatcttca aggaacaagg tagcaaagca aaaaaaagag ctctcgaact aacgagagct
    18241 tacgattgtg gta
//
LOCUS       DACENJ010000036        17788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.036, whole genome shotgun sequence.
ACCESSION   DACENJ010000036 DACENJ010000000
VERSION     DACENJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.036"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..191)
                     /locus_tag="IW748_000905"
     CDS             complement(<1..191)
                     /locus_tag="IW748_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1103828.1"
                     /translation="MKPNPVETGKRIKSIRSNFGMTMEQFADLIGNAQKGNINNWERG
                     FSLPNKERLIKIAELSNKT"
     gene            497..685
                     /locus_tag="IW748_000906"
     CDS             497..685
                     /locus_tag="IW748_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ1103829.1"
                     /translation="MPLTGKKMLKLAKKNGWKVVRITGSHHIVAKEGFPPVPIPVHSN
                     QDLKKGTEQKLLKDLGLK"
     gene            731..1108
                     /locus_tag="IW748_000907"
     CDS             731..1108
                     /locus_tag="IW748_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAQ1103830.1"
                     /translation="MKKSYPVIFSAEENGYSVVFPDFAGATQGDNFEEAMSNAKEFLD
                     GILAYYIDEDISVPSPTDIHQLNNLGNNKIVSLIQSDPSPFIQKKTVRKNVTVPEWLV
                     ARGEKDKINFSKVLTDALLKIYE"
     gene            complement(1668..2840)
                     /locus_tag="IW748_000908"
     CDS             complement(1668..2840)
                     /locus_tag="IW748_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103831.1"
                     /translation="MTKKIEVYVDGSQEERDSFFGFFWKNSREYVKALNMVYKQLMFE
                     ELIENSIKELDHEYIKRELSLEERIDNLLSNEVIDQKKLDKTKRELSKLRIAKNKEAR
                     DVLTQAIGLKSQTRIRDIAKKLDFDYSDFIDSAKMKASKDFSNDFVGIVTGQRTPRIY
                     RNKNKYGLLPFRGRDIKIHKADKFYISLPKGFRFKVNLGRKPRKARDSALTLERIMDG
                     QYKLNQSNLIFEGKNLFLNLTISFEKDYHKLDFKEGAELTIKFTDLCSGKCLVNNQSV
                     IDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIIQTAIRNKTQIICISEPDKNYGEWAYYQLKEQIINKGKRYGIEVKSE"
     gene            complement(3084..4052)
                     /locus_tag="IW748_000909"
     CDS             complement(3084..4052)
                     /locus_tag="IW748_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1103832.1"
                     /translation="MSLENEKPYYVVVGGVNGAGKSTIYQVQNLKRKELDTDRINADE
                     ILRSFGGNWKKQADINKSMREVLREIKGHFKEGKSFHHETTFGGNPYPDKMRLLKAKK
                     LGYESFLIYIGLDSPERAIERVKIRVAKGGHGVPEDLIRKRYEFSLRNLAELEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYAWVPKAIQEKNAETPISKQIMMDISQEEM
                     NPKETLERIYSASDDFISDRFSKEAQVDDFYRNHKNEIKERILSISFSTGVSQDELVG
                     QDKRRNSCKLAYINSVGELLVKTDSELLSMELNRDRNIQKTDFEVE"
     gene            complement(4049..4246)
                     /locus_tag="IW748_000910"
     CDS             complement(4049..4246)
                     /locus_tag="IW748_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103833.1"
                     /translation="MRAMTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLT
                     LSSFVTRVDQKKVINQKDFKI"
     gene            complement(4257..4574)
                     /locus_tag="IW748_000911"
     CDS             complement(4257..4574)
                     /locus_tag="IW748_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103834.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRFIEVNNLGIIEDYLDKLDEAIKQSIEKNGEDHILTVVLKDLKDRLEAEAEYF
                     NKD"
     gene            complement(4611..4829)
                     /locus_tag="IW748_000912"
     CDS             complement(4611..4829)
                     /locus_tag="IW748_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103835.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKAIGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            complement(4938..5129)
                     /locus_tag="IW748_000913"
     CDS             complement(4938..5129)
                     /locus_tag="IW748_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103836.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            complement(5291..5647)
                     /locus_tag="IW748_000914"
     CDS             complement(5291..5647)
                     /locus_tag="IW748_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103837.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            complement(5672..6676)
                     /locus_tag="IW748_000915"
     CDS             complement(5672..6676)
                     /locus_tag="IW748_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ1103838.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPVSVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            complement(6713..6973)
                     /locus_tag="IW748_000916"
     CDS             complement(6713..6973)
                     /locus_tag="IW748_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103839.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            complement(6982..8055)
                     /locus_tag="IW748_000917"
     CDS             complement(6982..8055)
                     /locus_tag="IW748_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103840.1"
                     /translation="MSNEQNYETGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            complement(8063..9889)
                     /locus_tag="IW748_000918"
     CDS             complement(8063..9889)
                     /locus_tag="IW748_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1103841.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATNEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSTPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKHDRLLLDRSPENCDY
                     VEKFVVESLKACENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            complement(10044..10910)
                     /locus_tag="IW748_000919"
     CDS             complement(10044..10910)
                     /locus_tag="IW748_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103842.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLVVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWKMVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYESTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            complement(11085..11348)
                     /locus_tag="IW748_000920"
     CDS             complement(11085..11348)
                     /locus_tag="IW748_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103843.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSTVKEFLYSNPKTALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            complement(11350..11679)
                     /locus_tag="IW748_000921"
     CDS             complement(11350..11679)
                     /locus_tag="IW748_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103844.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            complement(11701..13236)
                     /locus_tag="IW748_000922"
     CDS             complement(11701..13236)
                     /locus_tag="IW748_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103845.1"
                     /translation="MVGDDMTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDY
                     ISRQEAIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTR
                     KKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFL
                     VREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAM
                     ERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLEL
                     KQSVKDSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNY
                     MTKDNPKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLL
                     KQSEEKNLKSNELKNLLDGKKLNVKFDREKPEFKTKYAKQSYDDFQKRLEERQLAREK
                     NEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAREFEI
                     GMN"
     gene            complement(13303..13770)
                     /locus_tag="IW748_000923"
     CDS             complement(13303..13770)
                     /locus_tag="IW748_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103846.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKSNMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSTHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEQESSRLGEGTMFSSPSLRFTTD"
     gene            complement(13767..14063)
                     /locus_tag="IW748_000924"
     CDS             complement(13767..14063)
                     /locus_tag="IW748_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103847.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVNKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISERSDLNK"
     gene            complement(14414..14644)
                     /locus_tag="IW748_000925"
     CDS             complement(14414..14644)
                     /locus_tag="IW748_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103848.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
     gene            complement(14680..15576)
                     /locus_tag="IW748_000926"
     CDS             complement(14680..15576)
                     /locus_tag="IW748_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103849.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMIIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKSDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYQNLPKDFGVISVTVNPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKD
                     EKKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQFEEKIKQLEKSASRFE"
     gene            complement(15590..16240)
                     /locus_tag="IW748_000927"
     CDS             complement(15590..16240)
                     /locus_tag="IW748_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1103850.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATPSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDSK
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            complement(16255..17391)
                     /locus_tag="IW748_000928"
     CDS             complement(16255..17391)
                     /locus_tag="IW748_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103851.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            complement(17413..>17788)
                     /locus_tag="IW748_000929"
     CDS             complement(17413..>17788)
                     /locus_tag="IW748_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAQ1103852.1"
                     /translation="INSNNKYACEYVLSFEREMRKMRAGVYFATQTPEEIIPKDSGSE
                     VADVIRNIFNLCTFKCFFNLDSALLEDIKKVLGNTITDTEIMLLPELEVGQAVVQTSS
                     EDTYMINFDPYPEQIERFDGGQ"
BASE COUNT         5150 a         3389 c         2117 g         7132 t
ORIGIN      
        1 atagttttgt tacttaattc agcaatttta attaatcttt ctttgttggg aagggagaat
       61 cctcgttccc aattattaat atttcctttt tgagcatttc caattaaatc tgcaaattgt
      121 tccatagtca ttccaaaatt agaacgaatt gatttaattc ttttacctgt ttcaacagga
      181 tttggtttca tacaatcacc tcttttacag aatttggttt catagtatct tattcgctct
      241 ttgagttcta agttctcttt ctccaatttt tctatataat attcttgatc ctgtttcttg
      301 ttcaaaaaat gacctccaaa tttcacattg tgttacaccc tagtgttaca cctccaaacc
      361 ttattatact gtactttacc atatgataca atagggtata ccctattgtg acacttacat
      421 tctactttta atgtaaaaac tcttgacctt tatacgcata atgcgtataa taatatttgt
      481 aaggaggtag tcgcgaatgc cacttaccgg taaaaaaatg ctcaaactag ccaagaaaaa
      541 tggttggaaa gtggtgagga taactggcag ccatcatatt gtagccaaag aaggattccc
      601 acccgttcct attcctgttc atagtaatca ggatttaaaa aaagggacag agcagaagtt
      661 actaaaggac ttagggctga aatagcccag tcctttagtt cattggatta actcgaaagg
      721 agtggtggaa atgaaaaaaa gttaccctgt aattttttct gctgaagaaa atggctactc
      781 agtagttttt cctgattttg ctggagcaac acaaggagat aattttgaag aagctatgtc
      841 caatgctaaa gaatttcttg atgggattct agcttactat attgatgaag atatttcagt
      901 tccttctcca acagatattc atcaactaaa taatttgggc aataacaaaa ttgtttctct
      961 tattcaaagt gatccttctc catttatcca aaaaaagacc gtacgcaaaa acgtcacagt
     1021 cccagaatgg ttagtagcta gaggggaaaa agataaaata aatttttcta aagttttgac
     1081 agatgcgtta ctaaaaatat acgagtaaac gtaaatatat taataacaat ttagttgcct
     1141 aatgacatag atagcggaat taaaagtaaa atagtactca tttattgcag gttcccttag
     1201 aatcaataca ataaatgagt actatttaga ctgtttaact actagtaaga tatagcgatc
     1261 atcagtttca gggttatccc ttggtgacca tagttttgcg tatttttcaa ctcgttgcca
     1321 aatcaacgat tctatttttt tatttctact taactccaaa acggagtacc ttctttttct
     1381 tcgttagctc ctatttaaga gccatgtcgt tttatataac tctaaaacag aaactactga
     1441 tcaattcaat aaagccttat ttgagaactg tgttgtttta catggtttta aaacctgatt
     1501 tcccgcaaac ggtttatccg taacatttga gaactgtgtt gttttacatg gttctaaaac
     1561 ctcaacaata ttcgtccttc cgtctggtta cacaacttga cagagctaac ccttcagtga
     1621 gtgagtgtcc cctcactatt tcgttgccaa atcaacgatt tcttattcta ctcactttta
     1681 acttctattc catatctttt ccctttattt attatttgtt ctttcaactg ataataagcc
     1741 cattccccat aatttttatc tggttcagaa atacagatta tttgcgtctt attgcggata
     1801 gctgtttgaa taatttttgc cgaacattga ttattatagg ttttaacaaa attgctttct
     1861 cttttcttta tggcttccca tctatcttga tctaattttc tttttcgacc atgaccgcct
     1921 ttagaaaaaa ctcctcggct ttgctccctt gatttcattg ctttaatttt atttttgaag
     1981 ttacttatat atttgctgtc accaaaatca atcacagatt gattgttaac taaacattta
     2041 ccgctacaca aatcagtaaa ctttatggtt aattcagctc cttctttgaa atctagctta
     2101 tgataatctt tttcaaaaga aattgttaaa ttcaaaaata aatttttccc ctcaaagatt
     2161 agattactct ggtttaactt gtattgaccg tccataatgc gttctaaagt taaagcgcta
     2221 tctctggctt ttcttggttt tctacctaaa tttactttaa acctaaatcc tttaggtaaa
     2281 gagatataaa acttgtcagc tttgtgaatc ttaatatccc gacctctgaa cggtaacaat
     2341 ccatatttat ttttatttct atagattcta ggtgttcttt gcccagtaac aattccaaca
     2401 aaatcattgc taaaatcctt actagctttc attttagctg aatctataaa atccgaataa
     2461 tcaaagtcca atttttttgc tatatcacgt attctggttt gactttttaa acctatagct
     2521 tgtgtcaaaa cgtctctggc ttctttgttt ttcgcaattc gtagtttaga taattccctt
     2581 ttcgttttat ctaatttctt ttgatcgatc acttcattac ttagtaaatt atcaattcgc
     2641 tcttcaagac ttagctctct tttaatatac tcatgatcca attcttttat agaattttcg
     2701 atcaattctt caaacatcag ctgtttatat accatattca gcgcttttac atattctcta
     2761 ctatttttcc aaaaaaaacc aaaaaacgaa tctctttcct cttgtgatcc atcaacatat
     2821 acttctattt ttttagtcat agttattatt gtcaatttta gcacctcatt attatatatc
     2881 tggtatataa taatagtaac atatttccta gactataatc aatgatcaat tgtctacttg
     2941 tagtaattat attaaaaaca aaaactaaaa ataatatatt attccttctg actacatata
     3001 gtcactttat ccaaaaaata agaagtgctt tcgcactcct tattaaattt atttactatt
     3061 aattgattca gctacaactt cttttattca acttcaaaat cagttttttg gatatttcta
     3121 tctctattta attccatact taatagctca ctatcagttt ttactaaaag ctcacctaca
     3181 ctattaatat atgccaattt acaagaattt cttcttttat cttgacctac taattcatct
     3241 tgagataccc ctgtagaaaa actaattgat agaatccgtt ccttgatttc atttttatgg
     3301 ttcctataaa aatcatcgac ttgcgcttct tttgaaaacc gatcactaat aaaatcatca
     3361 cttgctgaat atattctttc aagggtttct tttggattca tctcttcttg agatatatcc
     3421 atcataattt gtttgcttat aggagtttca gcatttttct cttgaatagc tttgggaacc
     3481 caagcatatt cttgcgtatt attggctaca actgtatgat tttctttaac ataaatataa
     3541 gaaaactcat ctgaattatc atagatactt acattatcaa aatatttctc taactctgct
     3601 aagtttctta aagaaaattc gtatcttttt cttattaaat cttcaggtac accgtggcca
     3661 cctttagcaa cacggatttt aaccctttca atcgctcttt ctggtgaatc tagtcctata
     3721 tagattaaaa acgattcata acctaatttt ttagccttta gaagtctcat tttatcggga
     3781 tatggattac caccaaaagt cgtttcatgg tgaaaagatt ttccttcttt aaaatgacct
     3841 ttgatctctc ttagaacttc acgcatactt ttgtttatgt ctgcttgttt tttccaattt
     3901 ccgccaaaac ttctcaaaat ttcatctgca ttaattctat cagtatcaag ttcttttctt
     3961 tttaaatttt gaacttgata aatagtagac ttaccggcgc cgttaacgcc cccaacaact
     4021 acataatacg gtttttcatt ttctaaactc atattttaaa gtctttctga ttaattactt
     4081 ttttttgatc tactcgtgtc acaaaactag aaagtgtaag gtagaaagtc ctttcttcct
     4141 cactttctgc attttttaag gcttcatgaa aatcatctac agtcatattc aaaacatcag
     4201 gatacatttt ctttaattct tttacagcct ctgtcatagc tctcattccc ttcttattag
     4261 tctttattaa agtactctgc ttcagcttct agtctatctt tcaagtcttt taaaacaacc
     4321 gtaagtatat gatcttcacc atttttttca attgattgct ttatcgcttc atctaactta
     4381 tctaaatagt cttctataat tcctaaattg tttacttcga taaatcggaa gtccggtaaa
     4441 tttttcatcc gaataccgcc tttaggctgt tcctttacaa ataaagattt gtaatcatta
     4501 tattcattcc cattaataac caattctttc ataggctcta agtggataaa tttacgtgaa
     4561 gcatagtctt tcataataat ctctcccagt agaaaatctt cttttttcta ttatatcacg
     4621 ttctcaaagc gttgagttat tggattgttt aaaccatcaa gacacatcat gctgcatttt
     4681 ttcatcacta ccggtgcttt tccgattgct ttaactaccg ctacattagc taacaaacta
     4741 attacttctc cctcgataag ctctccgctc ccaataacaa cgccctgtac ttccatacct
     4801 actttaaacg tttctttttt atttaccatt attccgctct ccttccatga ctacttgtag
     4861 tcatttggat attttttagg ggaacggttt tccgttccct ttcatcaaat agaatccttc
     4921 atcatttcaa caaataatta actttcagtg taaaaaaatt cttcgtaatt accatcatga
     4981 aaaatatccc atctttgttc gtcattctca ctgaagtagg taaaataata tctgacagta
     5041 tctaacttat ttaattgctc tctggttttg taaatacaat ccaagtgatc ttgcattcga
     5101 tctgaccaaa acattcttgt ctgctccatc tattatctct cctttttatt aaatcaaggg
     5161 tttgagtctt tgactacttg tagtctatgg ctatatcata caggaatgac tacttgtagt
     5221 caatggtttt ctttagttta attaactgtc tattcgtagc caaataatgt aaaaaaacgc
     5281 ctagtttaca ctagacgttt aaacaattca ataattcttc gttatacccc attaagggtt
     5341 gaactgcttt aatacttact tgatcgtctt ctctaacggc ttctatgctt accccttttg
     5401 tatattggcc atctttctta tccgtccaaa tttgataata agtaagccca tatcgttcaa
     5461 taatctcaaa gagaatagtt attaattttt tttgtttaaa ttttggtaat tcaatacgca
     5521 atgatttttc ttgaatatcg aactctggat ttttattttt aagttgttct tcaataatca
     5581 tatagtctgt agacgtctta tctaaatcaa ttaatgaatc tgataagatt gctattgtat
     5641 cgttcataac acatcaacta ctttcttctt tttattccat ttcatataca ggagtattaa
     5701 gagataattt atgtgtgaag ttcacgattt ttgatacatc ttctaacttg aagcgtttaa
     5761 aagcttcttt tcctcttgtt aaattatcat tccaatcaaa tccctctcgc aaaggtacat
     5821 cgacttttac ttcactgatt cctatccctt ctcttccaat tcctttttct ttaaattcag
     5881 tttcaataaa agtacgtccg gctttatcat tatcaacaca aaggactgtt tctgaaggca
     5941 acgatttaaa cttattataa tgctctgtaa ccccatgcaa tacattacct acctttaatc
     6001 cattcatgct aacaaacgta gcattttcgg ctttttctgg cttttgtgta atgtacgaca
     6061 tcaaatctat ttcactttca aaaaaataca ttttatctgg ttgaccaaaa gttacgttaa
     6121 atcctgaata ctctttagag ttttcttgta tttttttcca tgtaacatcc ttttgtttgc
     6181 cttcatgaaa aaattttcta gttccttgtt ccgtacaccc aacgatttct tgatcttttt
     6241 tccataagaa caaaacattt ccttttttat cttgtttaat tagtccttta gataaaaaat
     6301 cttccactaa agctgtatcg attcttctct catttactaa ataagacttt gcaacagata
     6361 ccggcacttc attttctttc tgatattggt acggagcttt tttcactttt gtattgaacg
     6421 aatgaacttc ataatcacta tcttcaagtt tctgttttgc ttctttaaat gaacaatcgt
     6481 agtaggcttt gataaaatca aaaatatctc cccctaaatc ctttgagttc catttaaaaa
     6541 ggtttttacg atcactgatt acacaagaat catgatcttc taatctatag tattcccttg
     6601 aatcttgctt taaacctatc ccattttgtt tgcaaaaatc aacgatactt actttttttg
     6661 cctttttcaa atccaaacaa aaaccccctt tactagacca tcactagtaa aattacgata
     6721 aggtacactt gtatttttca taataatcct ctgtacagaa aataaagttt agcccatccc
     6781 taagagataa ctcccaaaag cctatcttct tgctcttttt ttctctaatt atcttttttc
     6841 taatactttt tccgtacttc aaagccattt taaaagcgat ttgtacatct tcacaaatta
     6901 caaaatgact attattaatt tgacgatatt caccttgcgt ttctaaaaaa tttctatttt
     6961 cttgttctat cattcattta tctactccat ttcaaatttt gttgtatttt caaaattagt
     7021 caatttcaaa atcttattct ccatttcttc agaaacaggt ggtatttcaa acgtatcatt
     7081 ttcaatttca ttataaattt ctctcattgc accgttgtaa gccatgatag acgtagcttt
     7141 tttgtattcc gtagaagaag tatctactgg aaaacctagc agttcttctt tatcgatctc
     7201 taacttttcg gataaatttg tcaatttcgt ttcaatttct tctttatgac tatcataaaa
     7261 ttcattaata tcactatcta atgctaatct ggaaactggt tcttccatgt tttctaagta
     7321 ttcaatgcgc tcttgccaag cttgtttgtc ataataatta gttgtttcta gctcttgctc
     7381 aaaatctttt atcacaactt ctcgaatggg aacttccgaa accttatgcc caacaaattt
     7441 atctgtcatg tcctcactac tttctaagct attccgttcc tcgtttaaat cgtgaatagt
     7501 ttcaatcttt actttatcag gctgtaacac tacgataact acatcctttt gtcttaactg
     7561 ttcttccgca ataatatgtg tatatttccc aacgtcataa aatgccttag cagtagcttc
     7621 atgatctcca attgtatttc caatgtcgtt aatcaaatct acgtcatttt cattctcact
     7681 aagagaagca atcacttctc tttttacaac tttttctccg taataactaa catcattgta
     7741 atcatttaaa atatcattac tttcttgtaa tttatctatt actttagaaa gctcttcatc
     7801 agtaatagtg cgttcattca tagaaatcgg cttctctaat tcaacattta cttcatatac
     7861 gaaaccatta tttccgttac taccctgttc agcgtacatt gtagccattt ctttattagg
     7921 tgttaagtac acaccaaatc cattagctgt tccgtttaaa cctttatgat ctaaactaaa
     7981 ttcatcaaat ttattcatac ttccatgaaa caggcgataa gttccgcctg tttcataatt
     8041 ttgttcatta ctcataattt tttcactcca tttcaaaaga acgttctgct gtttttacag
     8101 tcggcttttc caagttagaa ttttctggta tcttgaaagt tccttcatcc atttcatatt
     8161 ttactttagt tgcaaccagt ttataagcta ccactgaagc cacctcttta tatgactcat
     8221 tgtctattgt atctcttaaa tttgttcctg acacagattc aaaatctgta atcgtatttt
     8281 cgatttcttg tttatgagat tcgtaaaaat cttccgcagt catacaagag tttaattctt
     8341 ttacatcatt aatatttgtt tggttttcaa tttcttcttg ccagcatttt tcattatagt
     8401 aattgttagc attttcagat agattataaa ccgcttcatt tataacaact gatcttagcg
     8461 gttcgcttaa ttttaacgta tcatttttta gttcatgatt ctcgcaagct ttcaaacttt
     8521 ctacaacaaa cttttcaaca taatcacaat tttcaggtga tcgatctaat agtaaacgat
     8581 catgtttatt ttcacttgga ttctcaaaaa ctacaccata aacatcacat acatcaaatt
     8641 gtttcgtatc tgaataatat gtcacatagt tttctcctat tttagctgta acatcataat
     8701 tgctatgagt aagctcaata gaattttctc catactttcc tctataataa tccaccatcg
     8761 cttctaaata actaccgtgg attgttctat ccggatcgtt gattgtacta tcgtagaaat
     8821 tatgaaattc taaatctgat ttttgttgta tctgctcttt attctcatta ggaacaaagg
     8881 aattttttag tgcattaaat gctgtttgct gttcttccaa gactttctta tcaatcgtac
     8941 ttataaagtc aatagacgtt tctctaactt cttgcattat ctgtctaaag ttttttggat
     9001 caattttttc attattttta gtccaatttg caatataagg aatagtaaag tctgacgtat
     9061 caataccgta attgtgacaa acaataaatg aagtcatttc cgcttgaaac tctttaatcg
     9121 gtgttgagta ggaagaaccg tgttcattct tattgtgaag ctttttgtga gccaattcgt
     9181 gaatactggt agtaagattt tgtgttctgc tatttcttga atttaataca atttcttccc
     9241 taccatcaac atattgaata tattcacctc tagcgcttcc catttcacct ctaccagact
     9301 cgttcatatc ttttatagat atattcatag attcggccaa atgatttaca cctttttcta
     9361 attgactaac acttaattca ccatcaatat taaaattcca taccctgtta ggaaatattt
     9421 ttggcaaatc agcttcagtt gcattagttt gggaaatatc aaatacgtaa cccttcttaa
     9481 aatgcttgat ctttttgctt cttaattctc catttttgat tttttctttt tgctcgtttg
     9541 tcgcttcttt taacgtcttt tcctcaccat tttcatcaat aaaaaccgtt accggagcat
     9601 gcgcataaat gcctatccct ttttctttcg ctttaacttg aaatcctagt tttttccaac
     9661 catcataact tgctacagca attgctcctt caaatccctc atcgatcaac gataggttta
     9721 aaggactgta attgtgtatt ttagacatga acttggcaaa ctcgatcata tcttcagggt
     9781 tagactgata ttgctcgatt ttgctgttgg aagccttaat taaatcgtct agctcttttt
     9841 gtttttgctc tttcgttttc ggcttccagt ttcctttttt ataggccatc ttctatctcc
     9901 tttctttttt aggtaagtta aattacctaa ttaaattata ccagatatat aaaaactctt
     9961 ctatctaatt tcactgactt aatgcaaata aaaaagagat aagatagtta tagtaaccat
    10021 cttatccctt cttattttac catctactca actatactta cgtttttacc tttactatct
    10081 aattgaactt tgttatcttt agaagctact ttagtaagca aaagttgatc caattcatca
    10141 ttagaatatc ctgaataaaa attatctaaa actttcgttg tggcaactgg aattgataac
    10201 ttcaaatcca tactagtaga ttcataagat aattctcctt cagacgaaac ttcaccaaaa
    10261 ctatttaaat tccaaattgg actatctaaa tatttataag ataatttaac ggtttccatt
    10321 gaaggtgatt tgttttcatt atccatttta attgttgctg ataccatttt ccactccggc
    10381 tctgaatatt gaacaccatc gatctcttct atatccgcct gtattttagg tatactatct
    10441 tgaaaatcag caattttttg gtcattttga aactcttgtt tttcctcatt ttctaattct
    10501 gtttttacag aatctaaagt ggcattatag ctatcaatat cgcttttttt cttatcatta
    10561 aacttttgtt tatcagcgtt caatgcttgt tctattattt cttcatcact cattttagaa
    10621 actttttcca atgtaagctg ttccatatcc gttttaaata cttttgcttt gccatcttta
    10681 gtttggatca ccgcttttac atattcattt ttatcaatag gcgttttatt tttactactt
    10741 gtttcgacta ccaaccaaca acgtgagtct ttttgttctg cactatctat gaatgagata
    10801 ttatgtgctt catttgactt agcaccagct tcttctttgc cacatgcaga taataatagc
    10861 gcaaaactaa aaatcatagg tattgttgca ataactctta actttttcat ttataagcac
    10921 ctctttttta tcggtctgat atataatttt atattatcat atttttattc caatacacag
    10981 atactcttat ataatgtctt ttcatgtagt ttatcaaatt tctatttcaa accgcttaat
    11041 acaaatatca aagcccaaaa ttttctactg gaagctttta gagcctaaaa tatatttttt
    11101 atttgatcca aaacttcagg gaaatacaaa cctaatatca acacagcacc tataattaat
    11161 agcggaatca ttttcttaat aaaagagatc acagcaaaaa ctatcagtac tgcaactaat
    11221 actaaagctg ttttgggatt agaatataaa aactctttga ctgtagacgt tacatttaag
    11281 tatagttcaa tcaatacgcg aaaatctcca ttacttagtt tatccataac cgttctaaaa
    11341 tcgatcatat catttcctca ctctttccaa ttttattgct tcttttattg aagaagcaac
    11401 ttgttcatta ctatgcttat ctatatataa tggggaaaag taatttttat caaaaaagta
    11461 atttgcaaaa gctacatcgt cttctgtctt ccaattataa aaacagtaat tcccactaat
    11521 cggtatttca aaaagccccc aattctctag ctccatgttt tctttttcaa acaacactat
    11581 gatttttttt acatcttcta tagtttccga atcactgtta agtagctcac gtaaatatat
    11641 attttttgta ttactgcaca ctatatcgaa gtttttcata atttctattc tccgtttcta
    11701 ctaattcatt cctatttcaa attcacgtgc ttgtctcatt ttctcttgct ctatcattcg
    11761 ttgcgctact tcataatcat ttttcgcacg atacatattt aaatcatcat tgattactct
    11821 atcaattttt ctaataagct ttttatcttt aaatttttca gaaatcttct tgaagtggac
    11881 ttcatttttt tcacgtgcta attgtcgttc ttctaatctt ttttggaaat catcataact
    11941 ttgtttagca tatttcgttt taaattctgg tttttctcta tcaaacttaa cgtttaattt
    12001 ttttccgtct aatagatttt taagttcatt acttttcaaa tttttttctt cagattgctt
    12061 taaaagtttg atttgtccta ctacagcatt acccattcga gcattaaaat ctttttttct
    12121 atttaaataa tattcttgtg gattgtagcg tttaccgtag gttgtttgat atagctcatc
    12181 aatttcttta gtcatacgat catattcttt tctttttgga ttatcctttg tcatataatt
    12241 tgtgacttca tcaatctttt cttttgtttc ttcaggaagc tcattgtagc cacgtgttgt
    12301 actaggtatt tcttttttta attcctttag gtactttact aattctttgt catatcttga
    12361 attattttct acatataatt gaatcaaaga atcttttaca gactgtttaa gttctaaagg
    12421 aacttctttt ctcaacttag aaattttttc aaactcgttc gttctatcta atattttgct
    12481 accgaattta aaaatcatat cgtctaacgt agactgtttt ctcttacccc tagcttgtaa
    12541 aacaccatca tattccatta tttcacgagt atttttacgc tccatagctg ttacatgaat
    12601 atggatatgt tcagtatttc tgtgaatcgt tgcaaaccaa aaaggatcaa ctagttcttc
    12661 cttttcttgc atttttccca tcatagactt tgttgcttca tatagaacat tttcatttaa
    12721 ttcatttgtt tctggattat aatatccttc ccttactaaa aaatcattat cgaatgaaat
    12781 tacatcttgg aacatcaccg atccattatt ttttgcttct atgaccgatt cttttatttt
    12841 tgaaatgtct tttttagtga tatatctttt tgtattgcta aatgctccgt ttactatttc
    12901 attatctaca ttttctaaga tagctttttt acgtgtcata taatcaatat atttatccat
    12961 ttcacgtaaa tctttaatca ctggttgatc tgtttcctgt tctatttttt ccaaagcttt
    13021 ttctattcta gctaattcat ttagttcatc ttctgaataa tcatgatcta gtttatcttg
    13081 cctaatagcc tcttgtctgg aaatataatc tacaaatttt tgaagttcta ccatgtgatt
    13141 attctttttg ctttttccag tttcaaattt tgctttaaga ataatgctcg cactttttct
    13201 gttaaagata tgttcagtca tatcatcacc caccattttc aaaaattttt tctacaggcg
    13261 gttttgtact taatgctaag ttgcattaga attttttagt gttcaatccg ttgtaaatct
    13321 taatgacggt gaactaaaca ttgttccctc tcccaaacga ctactttctt gttctggttc
    13381 agcacgctct tcttctaatt ctttactagc taatccacta ttaattaata gtttatagac
    13441 agactcttct atactattta attgttcact ataatcttta tgtgtactgt cttctttttt
    13501 attaaagttt tcaagttgct gtttttgcaa ttctattatc gtttcaagtt cgctaatttt
    13561 taattttgct tcttctaagt ttttagctaa ctcttctttt tctggatcac ggaaattttg
    13621 attggctttt gttaacattg gtaaatttga aacaacataa cggaacatat tggattttgt
    13681 ttgaaatact gacttgttta cttcaaagca cttattgaat ttttctttta catcttctgt
    13741 tgctgatact ttgaaataag ggtagttcat ttatttaaat cactcctttc gctaattaaa
    13801 aaattgaata agcgttcata aactgaagtt aactcgctaa tactttctac catttgttca
    13861 tttgacaata aaagtttttc aactactaaa tcaaattctg actttttata gtcaatcaaa
    13921 tcgatctcgc tatcttggcg aatgattgct aaaaaatagt cattcctact aaactttttt
    13981 ccggtacgtt cttgaatttt attaactttt ttatcaatct cttttacata ttgtggatca
    14041 attcctctga ttgaaacatc catacacaca cctctttcaa gctgttttta tctatctggt
    14101 atatattatt atatcaaagt ccaaaggaaa attaaagatt taaagacaag accaaggaat
    14161 taatacaaag agcataaggg aaaactgata gcaccttcct aaaggaaggt ggctaagttg
    14221 gctgtgccaa ctggttttct ttcaaaatag cttcatattt tttgctatca caaaaaaatc
    14281 cattttcgac ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt
    14341 agttttggtc atagtaacta aagcttttac gcccaaaaaa agagtggcaa gttttacctt
    14401 gccactctct tttctattca caaaattggt ttaaattcgc tttttttagc acttcgttta
    14461 tatcatagca atcataatat ttacacaaca tcattcctaa ttttaacgct tgttcctctt
    14521 tatatttttt aagctctgca ttttttttgc gcttaatcgc ttttctcttt tctttatatt
    14581 ccgcttcaat tctcattaat tcttcttcag ctttctcttg aatagtgaca atagaattac
    14641 acattctctc taaactccct taaaataatt taaacacgat cactcaaatc ggcttgcaga
    14701 cttttctagt tgctttattt tttcctcaaa ttgtttgatt ttattttcac tttcctctat
    14761 tgaagattta ttattttcaa tagtcgtctg catagaactg atttcatttt ttgtatctgt
    14821 ttgttcgtct agggtttgaa attctaagtt ttgattagct ttatcaatat ctttttgcac
    14881 tactttattt gctaaattca ttttttcaat tgattttttc tcatctttta tagaattttc
    14941 tatagcttta atctgatatt taaaattatc tttttgcaaa tcagaagtac tttcaacttc
    15001 caatttagca tttgatttaa tatctttgtc tactgcatag aattttattt ccttttcttt
    15061 taagtcattt gaaggttcta attcaggata aatatatcta gggttaactg tcacagaaat
    15121 tacaccaaaa tctttaggca gattttgata ataaattacc gtgtattcat ctgacaattt
    15181 tacaactttt gtcttaacat cttcattatt tttgttcgtt ataaaattta atttatagtc
    15241 acttttaata ttacttagtg atttagtacc tgtatcatct tgataccgca atactaattt
    15301 aaacagtccg gcttccctat ttatttccat atcatcaaca tacatattca aagaagacgt
    15361 tacattttgg gctgaactca tttttacagt ttcaaaatca taacttttcc ccatcacata
    15421 aggagaaaaa acgattatca ttcctattgc taaaaatcca agtatagcac ctaaatataa
    15481 ttttactcct ttcgtttttt tatatacttt gtcaatttgt gccttatttt ttttatttgg
    15541 ttccttctta ttatgaatgg aatatttctt attcatatac ttcacctcac tattttaagt
    15601 acttgatttc ataattatct accaacactt ggccgtcttt attggttact tcaaaaagca
    15661 tatagactgt attttctgta tctccactag aagattctgt tttataattt acttcagcaa
    15721 aataattaat agtattggcg ttaacataat tcgtatataa ttttgaatct ttatatttac
    15781 ttttgaatgt tggatcactt gaatatgctt ctcctttttg agtagtagat ttactttcta
    15841 aagtcttttt actcgttgta atcatgtcgt ccggcacgat agaatcagct acagaaggag
    15901 tagctatttt ctctaaatca gctttctttt cttctacttt ttcaatatca tataccggat
    15961 atagctcaac aaatttctta gcaacttcag caatttgttt atttaattca ctgccatcat
    16021 cattagttaa gttcgcttct acatttgcat ttagatcact aatattttta cttaacttgc
    16081 tattttgttc ttctagttgt gtaacttctt gatccaattt tttatttttt gcttgaatgt
    16141 tacttgtatg aagttgagat aacaatccac ctaaaactaa aataataatt aaaacaccac
    16201 aaatatattt ccatttatta tcattttttc tcttaatcat ctattttctt cctcctattt
    16261 tttgactcta cgaatctctg gactatgtga ttgccaatat gaatcttgcc aattgtgata
    16321 accaattccg cttgaattac tgtcatacat agttgtttca ttgacataaa tacctacgtg
    16381 agaaatgaaa tcaggcgctc catatgttcc tctaaagaaa atcagatcac cagcttttgc
    16441 ttctttaatt gatactgctt cagttgcttc atactgcgtt actgtataac tatctattgt
    16501 aattccggcg ctaaattgat aaagataagc tgttaatccg ctacaatcaa aacctgtcgt
    16561 tggattttta ccgccccaaa cataaggcca accttgatat ttttccattt ctgttttcat
    16621 ttttccccaa aattctgaag gattagccgg attaccacta cccccaccga tatactgttt
    16681 aactaactct gcatagtaaa aattaccacc atttaaatac aaatatgttt ttccatattt
    16741 aattgatact tcatttacat atgaataagt ctgtcctgta gtatttccta aacttggagc
    16801 aacaacattt ttagagtagg cttcagccgt ttcgatagta tgtgtttttt tgttttttcc
    16861 taagtagtgc atgtaagcta ttccgtaatt gtaagcttga actattccat catagttact
    16921 ttccatgcca taacttttag caatgttata cacattactc aaatgtttta gcccttgatc
    16981 gatactttct tcatattgaa gtgtgtttac aggcaatccg gctgattcgc tactttgcat
    17041 taaatccggc aatttacctc cggattcaca ttgcataatt gctaaagcat aaggtactaa
    17101 ttctttaatt ccgtattttt cagcatattt ttctaccgtt ttttgccatt tttgaacttc
    17161 aggtgacaac ggtttgttgc caccaatgac ttctgcacct cctgtattag ttgcagtttg
    17221 tgttccttgt aatgtagcta aagaacctat aactaaaagg ccaaccaatg ttatcagtcc
    17281 tataactacc cctattttta tttttaaagc aagacctatt ttctttgata cttgcttcat
    17341 cccttgacga gcgagtgttg aagcaacatt attatttttg ttctgctcca ttaaattcac
    17401 ctccgatttc tattattgac caccatcgaa tcgttcaatt tgctctggat atggatcaaa
    17461 attaatcata taggtgtctt cactggaagt ttgaactaca gcttgtccta cttctaattc
    17521 tggtaaaagc ataatttctg tatcagtaat tgtatttcct aaaacttttt ttatgtcttc
    17581 caacaatgca gaatctaaat taaagaagca tttaaaagta catagattaa aaatgtttct
    17641 aatcacatct gctacttctg atccagaatc tttagggata atttcttcag gcgtttgagt
    17701 tgcaaaataa acacctgcac gcatttttcg catttctcgc tcaaaactta gtacatattc
    17761 acaagcatac ttgttatttg aattgata
//
LOCUS       DACENJ010000037        17247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.037, whole genome shotgun sequence.
ACCESSION   DACENJ010000037 DACENJ010000000
VERSION     DACENJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.037"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..276)
                     /locus_tag="IW748_000930"
     CDS             complement(<1..276)
                     /locus_tag="IW748_000930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103853.1"
                     /translation="MKRIVKIFGILGVFLIGLSTFVSAGISVDASETNNFLVKQQETE
                     INIIQNNLATHKDSKGNRLVYIIDESDLKSSLDRINSEITVSEIQQYL"
     gene            complement(320..652)
                     /locus_tag="IW748_000931"
     CDS             complement(320..652)
                     /locus_tag="IW748_000931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103854.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRKEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLVWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(666..896)
                     /locus_tag="IW748_000932"
     CDS             complement(666..896)
                     /locus_tag="IW748_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103855.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKVYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1118..1654)
                     /locus_tag="IW748_000933"
     CDS             complement(1118..1654)
                     /locus_tag="IW748_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103856.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLTTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDYMT
                     DRCIKDNSIDVDPHVQMKTTGKLHQVTQDIENPDQYLLFGEETTNEVTQFVLLQLKVD
                     QEAQKIDAIKIYYVRSVY"
     gene            complement(1675..2766)
                     /locus_tag="IW748_000934"
     CDS             complement(1675..2766)
                     /locus_tag="IW748_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1103857.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNWE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESTYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKDCYDYYGFLVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSDGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2774..4984)
                     /locus_tag="IW748_000935"
     CDS             complement(2774..4984)
                     /locus_tag="IW748_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103858.1"
                     /translation="MAYIKRKEKKWCVWLFLTVGFGLFFVGGLVYAVEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPAFDYMIMNTLKSFLSLMFQKSFYSVIILVAFLIY
                     NIVDDLILMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGGQVINPFELGGRSNQDLPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIHQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQRQIQRSSESKPSISVP
                     KSLQPAFSSFNNPVEQSKKIEKKNETLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5000..7528)
                     /locus_tag="IW748_000936"
     CDS             complement(5000..7528)
                     /locus_tag="IW748_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1103859.1"
                     /translation="MVLVKEPVKALKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFYDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRMPKEHKGLLQLEDELGTSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTTIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAIGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQIEEQDTLIMQ"
     gene            complement(7582..7992)
                     /locus_tag="IW748_000937"
     CDS             complement(7582..7992)
                     /locus_tag="IW748_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103860.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFFIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFANKYVYLED"
     gene            complement(8005..8223)
                     /locus_tag="IW748_000938"
     CDS             complement(8005..8223)
                     /locus_tag="IW748_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103861.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8220..9212)
                     /locus_tag="IW748_000939"
     CDS             complement(8220..9212)
                     /locus_tag="IW748_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1103862.1"
                     /translation="MKKRQLRRKEKVYTEKVPNQRKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSQSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQEILKKEFYAQNFIQEPGSVSGIRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMDPENKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKTFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDRKGDERK"
     gene            complement(9291..10202)
                     /locus_tag="IW748_000940"
     CDS             complement(9291..10202)
                     /locus_tag="IW748_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1103863.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLSDVETELNGGTTEKPAEPEHLQPEKPEKEPVAPEPEIPESTLPTDPIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSETNTDSSSTEQPTMSFTSEEPKEEPKQPAEKSEAIPK
                     ENKPIVPAKEVTVSVTPNGEITTNNSQGMSVPIVTSNVEELTHIPTPTTPLKAEGGQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVLS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10221..10565)
                     /locus_tag="IW748_000941"
     CDS             complement(10221..10565)
                     /locus_tag="IW748_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103864.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLGPRTERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10585..11142)
                     /locus_tag="IW748_000942"
     CDS             complement(10585..11142)
                     /locus_tag="IW748_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103865.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIRVIKKLCTIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFHKREKNQMSAEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEKLMMDFSDLYRGYECSVIQER"
     gene            complement(11222..11722)
                     /locus_tag="IW748_000943"
     CDS             complement(11222..11722)
                     /locus_tag="IW748_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103866.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPKEYPIEKRELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPINFLKKC
                     HLRILP"
     gene            complement(11723..12268)
                     /locus_tag="IW748_000944"
     CDS             complement(11723..12268)
                     /locus_tag="IW748_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103867.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGTLKFIRFYRFSLPVPAILAVK
                     KGCDKMKIYENCVAALSNEKPLVEEISLATVTWEMNRQPTEEQTYLSLEEIELELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12265..13521)
                     /locus_tag="IW748_000945"
     CDS             complement(12265..13521)
                     /locus_tag="IW748_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1103868.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKKKIPEDFEKRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKIVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKQTVTQNVLVQFSGQGCREYEEVMREQRTD
                     WRTWLYSIWKNQGKLEAGYKRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKKFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISEIEAL
                     TKYGIYNRYELRLANEKATQAVEAFILDHSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIENAELNDEQQSYLEWMGTSGGKIA"
     gene            complement(13884..14198)
                     /locus_tag="IW748_000946"
     CDS             complement(13884..14198)
                     /locus_tag="IW748_000946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103869.1"
                     /translation="MGEVLYRVSSAAGEISPDFAVRRLYEWINKVEYYTKGSYVFRRI
                     ERETLFVTRNQIVLTKEDILRFRQVYRLCKEKNIQLRLAILQCFAPEQYKELQEKEDS
                     LV"
     gene            complement(14325..15518)
                     /locus_tag="IW748_000947"
                     /pseudo
     CDS             complement(14325..15518)
                     /locus_tag="IW748_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            complement(15708..16064)
                     /locus_tag="IW748_000948"
     CDS             complement(15708..16064)
                     /locus_tag="IW748_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103870.1"
                     /translation="MNLDKTNQTLREIMVCCQNIQQLIEYFGDNPTIFIQTNEYICAV
                     IGQCMFLRDLVRFSLPYQWKQDHQEVVWIRVVRICNMCAHPYKNRLNLFRLWFFIKCD
                     IPVLVAFCQKFLAQEK"
     gene            complement(16130..16582)
                     /locus_tag="IW748_000949"
     CDS             complement(16130..16582)
                     /locus_tag="IW748_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103871.1"
                     /translation="MGLKFPNNYDITEALDETCIGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNSNPKQSQEKGLDQKQPSADSKEKEKK"
     gene            complement(16584..16949)
                     /locus_tag="IW748_000950"
     CDS             complement(16584..16949)
                     /locus_tag="IW748_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103872.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            complement(16966..17220)
                     /locus_tag="IW748_000951"
     CDS             complement(16966..17220)
                     /locus_tag="IW748_000951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103873.1"
                     /translation="MEKELLLGFYRKENNGGKKNMEKKNINICFLSGKECTYEECTDI
                     SVHERDGMLIVDMSPSDEPFVVKHYFPLCNIESFRIDCEF"
BASE COUNT         5205 a         3760 c         2462 g         5820 t
ORIGIN      
        1 tagatactgt tggatttcag aaactgtaat ttcagaatta attctatcta aacttgattt
       61 taaatcactc tcatcaataa tatatactaa tcgatttcct ttagaatctt tatgtgtggc
      121 taaattattt tgaatgatat ttatttcagt ttcttgttgt tttactaaaa aattatttgt
      181 ttcagaagca tcgacactta ttccagcgct aacaaaagta cttaaaccta ttaagaaaac
      241 acctaaaata ccaaatattt taactattct tttcataatt tttcctactt aaaaataagt
      301 tatttttaaa atatcttgat tatgaattca tcccccaaag gccttttaag attgatacag
      361 cagtccgttc acttctttta gacttgtcat ataagtcttt taaatgatcc cataccagac
      421 tagtttgtct atctagcttc aatccattaa acgctaatga acggttcatt ttttctatta
      481 ctttagataa agctacagaa atttttatac cttcacaacg cgcatcttgg atttcaataa
      541 tagtatcatc aattagtttt tgaatttctt tcctatcaac atattcaatc ttagaaagag
      601 aatcttttaa agctactaat gattcaacga tttctttttt ctcttcagac attctcttcc
      661 tccttctagt gacccgtaaa gccatgtaga gcagagtcca ttgaaccagt aattacacta
      721 tctacaacgc cattccaatt tactctacat tttccatttt tcagatttgt cggtccatac
      781 ggatagactt tttgacattc aatgttcgga ttataccaga taggattata ccattcagcc
      841 gcagaagcag attcagtcga tacaaacaat cccacaccaa caaaaccaat acacatcaca
      901 ctatagccaa ttaatttttt catctcgtat ccatctccta aaaaatagaa tagttcttgt
      961 agttattcat aagacatatt ttttccgttt ttgttttatc ctttctgtcc tataacaact
     1021 tttttcaagt aaagaatgac atacttcttc ttaaaaaaac agtctgtttt tttctatttt
     1081 ttcggtgcat aataaatatg agtgttcatt ttttatttta atacacactt cgtacataat
     1141 aaatcttgat cgcatcaatc ttttgagctt cttgatctac tttcaattga agtaatacaa
     1201 attgagtgac ctcattcgtt gtttcttctc caaataacaa atattgatcc ggattctcta
     1261 tatcttgtgt cacctggtga agtttcccag ttgttttcat ttgtacatgc gggtctacat
     1321 caatactatt gtctttaata caacgatccg tcatatagtc cctcacagct tgattgcgtt
     1381 tataaacatc tgtgaaatta ctccattttc tagcaaaatc ttttaagaaa ttttttgtca
     1441 tttcatctat ttgttgcgct gtctcgtctt ctaatgaacc aatttgactg ttactttcag
     1501 tactttgaag agttccactt gtttctttta ctgcttcgat tgtttcttct gtcgttgtca
     1561 attgtgtttt ttgtttttca gaagaaaacc aatgacaacc agtaagacct atcaaacata
     1621 accaactaaa tactaaaatt attccttttt tcacgcttct agacactgcc tttcttattt
     1681 caccttacga accagactac gaatttttgt ttgattgtac gtgcgttgat accgagccat
     1741 aatccgtcct tgctcggcat tctgttccac actagagaaa tgttgtccgc catctgaaac
     1801 atctgttata attccggtgt gaccatagat tccaggagac aatgcaccac ctgcttgcca
     1861 attcaccaca tctcctgctt gtaaatcact tggttttggg ttcatgatca cttgccaacc
     1921 ataggctgtc caatcataat cttcccctat taacatcgca aacatgtgtc cagaacccat
     1981 taactgtggg ccacctagtt gggatacata ccacgccgtg ccaccataac actctccatt
     2041 atttaccgat cgtccaatga tccgatctaa aactttcgca ttacctttac ttttccctgc
     2101 aacaagtgac gatttttttg ttggtaggct attccaatgt ttgatcgcaa aagtatcata
     2161 ttctgttaag ttataagact ttatgatact catcgctcct tggtaatacg gactgccttc
     2221 ggtaatacct gccgccgcat agcctccacg acctagaatc aataaacaac tcccaggatc
     2281 tttattgccc acacattgat taaagttgct ttgactcgcc accaaaaagc cgtaatagtc
     2341 ataacaatct ttcaggttcg gaaacttcat atagaagcca ccttcagaac caccaatccc
     2401 ccgtgcagaa ccttgtggat agggacaacc aggaaaccag gtaatcccaa agaagtttgt
     2461 atcgtttctt ccagattgtg attgtccata agtcgattca taacctaatt gagaaagaat
     2521 cacacttggt aataactgat actttaccgc tgcaccgact gctgcttgcg catgttcatc
     2581 tgtgatcgga ccacgtcctt taatctctga aaaaatcggt aatccttcgg tatattccca
     2641 attaaaagcg acagctagac ctgtagcctc tcctccatcc tcggaaagtt ctcctgaaaa
     2701 aaagaatggc aatagaaaaa agggaagaat aaagaaaagc agacaaccta gacacccttt
     2761 cttcatttct tcctcatcct ttcttcgata atggtttttg aactgttcgt ccttttggta
     2821 aagtaaaatc ggttcctggt tttgcgtcca aaatcggcaa tgtttcattt ttcttttcta
     2881 tttttttcga ttgctctact ggattattaa aagaagaaaa tgcaggttgt aaggattttg
     2941 gtacggaaat cgacggttta ctttcagacg aacgttgtat ctgtcgttgc acagaacgat
     3001 ttcctgatag agtggctacc gacgacttag gaacagagta ttgattcaat aactgctgca
     3061 ttgctggata actcccattt ttcgcctgat ttttcacttg gtgaatctgt tgtgaaagag
     3121 aaccagcagc tgttaattgt ggcacgggta cttttccagc aggcaacgga atcgattgac
     3181 caacaaaagc gataccttta tttaacaatg gttttctttg attattccct tgttgcttac
     3241 tcgattgtgc caacgattgt acctgttgtt tcggtcttac tgaattcatg gacgaatttg
     3301 gaatcaaacc atttgaaaga ccagaattcc actctgtttt tgatttggta gaagacattt
     3361 caggctctac cccatgataa cgaacatgac gaccactata aggcaagtct tgatttgaac
     3421 gcccacctag ttcaaaggga ttaatcactt gccccccttt taccgattct tgtggtgtac
     3481 gtttttgttg aaacttttga aatccatgta tcgctgcaga agcgagtgga ttcgttgctc
     3541 ggtacacatt ccctgctaat cctgcacctt tgattgccaa attttgccca gcagttgata
     3601 gttgaccagg cagttgtttc atttgagaca aagaatgact tgctatgttt ttcgtgttat
     3661 ttatggtttg atctgcagct cctaacccta gtttctttaa taaatctttc cgttttacaa
     3721 tgagtaaaat tgataggagt gctttgacaa acatatttcc taaaaaacca aaaacagtat
     3781 tcattagaat taaatcgtct actatgttat agatcaaaaa tgccaccaaa ataatcacac
     3841 tgtaaaaaga tttttgaaac atcaagctca aaaaggactt taaggtattc atgatcatat
     3901 aatcaaatgc tggaacaaaa gaagcgagta atgaaaaagg aatcacaagt aataaacaca
     3961 acaccaacat ttgaaagata aaacgaatca tgccaatcac tacaagtggt aatccaatac
     4021 ttagattaag caatggcacc atagaaccaa cgactgcttt ataaacagct ttagttggtt
     4081 gaatatagac acgataagcc ttctttttcc ctctatcttc tcctgcagca gtggtcactt
     4141 ttgtctcaat atcagataat tcttcacttg acgcttcctt ggctagaaat tcttgtggtt
     4201 caatctctgc ttgatacaat tcttctaaat ttgttgtccc ataatttaac aatagatacg
     4261 gatcaacaac agccgcttta taataaagtt ggcgcacttt ttgaatggct tccttttgtg
     4321 tcgttggcac taaaacacca ttgacatctt ttgtcgccaa ttcatctaga ctatttgtag
     4381 cagaaaaaat aaagccttct acctccacgg acaaatcatt taacccttta gtaaaagctt
     4441 ctcctttact aggtacgtta ctaccatagc caaaccaaac cattgaaaaa atgacaacaa
     4501 agagaaatcg aataaacacc atttttgcgt aatgtttccc tctaaatata cgaagagtaa
     4561 atagatataa cacatacaag acgacaatcc ccgcaccaat aggatcgtat attttcgaat
     4621 aaatagtatc cgaaaaagtg aaaaactggt gaattaactc gttcattgca gctccttcat
     4681 agagcttctc aatgacaaaa tcaaggcctt gccaaatcaa tttacttaaa ctaaagaaca
     4741 tattcgtcat catttttaga aaatctgtgc catttgctcc aaataacatt ttgcctaatg
     4801 aaaattcact atcagataaa taagaagaaa aggtatcaat atgaaatttc ccgatctcat
     4861 agatcgattg atccacctgt ttttctaccg catataccaa tccaccaaca aaaaacagtc
     4921 cgaatccaac cgttaaaaaa agccaaacgc accatttctt ttccttacgc tttatataag
     4981 ccattgtttc acctcgtttc tattgcatga ttaacgtatc ctgttcttct atttgagagt
     5041 gacttgtttt ttccgttgtt tgtaatgcag cggtcatttc ttcaaacaaa ctgtgtacat
     5101 tgagttttcc tacacgtcca tagggatcta acatccaaca ctgtccttta atcatatggc
     5161 tcatttcaga taagttcgct tcatttactt ccaaacgtaa atgagtcaaa atcttttctc
     5221 gctcactgtc ttcatcaaaa gcgaagacaa caccaaaatt ccctttttct ttatctgaac
     5281 ctaagtcgcc aataaattgt gtatctagca ctaattgatt catctgtgaa cgccccacac
     5341 gcgccattgc tgcaataata ctacgtcccg cgttcgaatt attaaatacc catgcttctg
     5401 taaaataaat cgctgttttc ttactactat ctcgttttcc aaacatttca caaaaacgac
     5461 ctaaactaat catcaaacac aaagactttc gatccgcatc tgtatatagt tctggtcgga
     5521 cattgtcatt tggtaatttc aaatcttgga tctctaaaat cgttgttttg gcattaaagt
     5581 ctaatcccgg gttttctcca taagaaaatc ctaaacgtaa aatcgaatct tctaccgtca
     5641 agcgtaaaaa ctcaccatat tctcgaacct ctttttgttc catttgttct aaatactcga
     5701 ccacatgcat caagccaact tgttgtccgg cttctttttg aactaaaact tcttggattc
     5761 cctttaatac cgcccctttt aaaaggtgat tggtacctaa cggacttaac tcatcaatca
     5821 tatcctgggc tacttgtttc gcatctactc gggataaaca aacaattgga tctaatacgc
     5881 cataattacg gggatttgta gcatctaacg tgacataact cattttttgc aaatgttcca
     5941 caaacaaagg gtaatgtttc tgataatacg ggtcttgaat gagtttcgta aaccatttgc
     6001 gcttttctcc tttgggatcc acaaataaac tttgtacatt cataaacgaa ctatacaaaa
     6061 agagcaatcc aactaaaaag gtcttccctt taccggtttt tccagttact gctacatgag
     6121 gcgcatcaaa caacgcacct ttgattcctt ccgcaatagc tagaagattc aaaaagacaa
     6181 aacgatttga cgcataaatt aattcttcta atggtgtttt ttctcctcga gaaccaccat
     6241 cccctaatgt atcttgccga ccaatatacc agccggattc catacctaat tctgatactg
     6301 ctgaaaacaa ccattcgcca attgcttcta cagttgttcg gtgcttccaa tgcaactgtc
     6361 gacccaatgg ttgccctaat aaacatttat agaacaattc tcgttgatca ttttttgcac
     6421 ggtggatatg gatagtcgta tgaagctgtt gaataatcgt tgcactattt tgacgaactt
     6481 cttcaaccgt ttgaccgtat acaataaaac atgcgatcca atcgacgaca ggttggccat
     6541 tatctactcg ttgctttaaa tgttttacta atccccgaac agttaataag cgttcgctag
     6601 gttcttcatc gttttgaacc aattccttat gttctgtttt gaaccgttcc gatagccaat
     6661 ttagttttaa accaatacct ccctgtcctt tcaagcgtgg atagtgaatt ttcattcgta
     6721 attctgctgg gaaagaaaga tcctgtgcaa attgaaatag atcacagttt ctcatatttt
     6781 caggtgtttc tccaataggt aacaacgcta aataactagt ccccaactca tcttctaatt
     6841 gaagaaggcc tttatgttcc ttcggcatac gtacatacgt tccttgattg tctttcagta
     6901 cttcccaatt attttgttct tctactttat gagccattcc tcgaatatat tgcagacgat
     6961 tgagatacaa cagctctttt tttactaagg gacgaataga aaagctataa agtaattgtg
     7021 tcacttcttt tacataagga tcactttcag aaaacaaagt ttctaaatca tctacttcta
     7081 atccaaataa gcgtgctaaa cttcctgcta catctaacca gattagattt agtacatcat
     7141 aaaaactctc cacggttgct ttttctttga ctttgattcc taaaataaag gaaggacgat
     7201 agacttgacc ttctcgttcc aacaatgtca ctgtttcttt tgcataatac gcaccaattg
     7261 cttgactatc gggagaaaat ccctttccta atgtgtcaaa tttcgtccgt aaattcattg
     7321 ttcgtggata catcaccaac tccacatctt cataatctgc taaaagtaac agtaaacgag
     7381 ccatcatttc ttttatttcc tgtgtctttt ttggtgtgtt ttttgaaatg gttcgtggaa
     7441 aaacatgata gtaggcccag atacaatcct cgccatttaa caaacaattt ttctttaatg
     7501 ctttcactgg ttcttttaca agtaccaata gatctcacct cccaattaaa aaaaggaaca
     7561 accagttgat tgtccctttt tctaatcctc taaataaaca tacttattcg caaaaagtaa
     7621 ttcttccaat gcattatttg ataccttgtc ccagttataa tacacacaat caccattcat
     7681 catacgcact acataacaaa accaatccca taaataaaaa tgcacttttt ttccatcagg
     7741 ttttagtttt gccagtgcta acacacataa atagggtagc cctataaaaa aaacaacccc
     7801 tgctggtgga aaaaaagaaa taacccaacc aataaatgaa cgtaaaaata aatattcaaa
     7861 gattacgacc aataaaaaag ttgctgcatt gactaatgca actggaaatg gaagacgcca
     7921 attgcttgta atctgttgta cgcgataagg ctccgcataa acaatcgaat aatcgtaagg
     7981 ttcccaatcc acttcactat cctcctaatc cataaataat ttttcaatga ttttaccaaa
     8041 cgcatcaaat acacgcgttg gatcatttaa gaagaaatag actgcggcac ctgctgcgat
     8101 agatattaat gcatttccta cttttgattt cataaaattt cgcaatccca gataaacaac
     8161 caccattatt aaaaaatagc caccttgcgt taatataaaa tctacaattc ccttcagtgt
     8221 catttccgtt cgtctccttt cctatccaac caattatgtg tgagtttttc aacataatac
     8281 tgatttgttt ttttcgtaat aacaagcgtc atttgttctt tttgtaaaag tgacgtatca
     8341 ccatctcgaa agacgactgt tgcttttaca ataaaagttt ttttcgtttc tttataacgc
     8401 aatactttca tctctacttg atcaacttta gctgtctgat tgagtccttc aggttctttc
     8461 atcatgtatg acatatcttt tgcattgttt ttcgcatact tatctaagaa atcttccaca
     8521 aatccttcca catctttcgc ttccgacgca tcaaccgttt cgtataatgt gtcatcataa
     8581 gtaatcattc gttgtttttc tgtattcttt ggtagtgccg taaaataagg ataagcaata
     8641 atacgacagc ccttgtttga aatagaaaag ggaagattga gtacttgtgt gattgtcaca
     8701 cttgtttctt tcttattctc tggatccatc gagcgaatat cataagttac tttataacta
     8761 gctgttggaa tggtttcaat ttcttttaat ccaataaacg aagcagcagt taattttcga
     8821 atacctgaaa cacttcctgg ttcttgaata aagttttgtg cataaaactc tttttttaat
     8881 atttcttgac gttgttgttc ttgttcatga ttatcgaatg tattcatata caaagcaaca
     8941 aaacgattca taaaggattc aatttcaggt gtgtacgtta attctgactg actttgtact
     9001 tctttctttt gtaattgttg gatttcatct ttcaaaaacg acacttgaac cttcgttttc
     9061 atggattgaa aaaatgccaa gccccctgaa aaacaaagaa ataaaaccaa acaccaaatc
     9121 catttcactg ctttttttga atggattttc ctttgattcg gtactttctc tgtataaact
     9181 ttttctttcc gccgtagttg tcgttttttc ataagctact ccttctagta tgtttttact
     9241 agtacgtacg attgacaacg ataagcttgt ttttctgaaa aatagtccat tcaattaccc
     9301 tcattttcta ttccttttct ctttctttta tatccaacaa atccagcaat acttgtcaac
     9361 actcctccta gaacagaaag tacaacatgt atttcttcac cagtctttgg taagactttt
     9421 gtttttccat ctgctgtttt gacttcaata ttgccacttg gaagtttctt tacaggaatc
     9481 gacaaatcct ttaccaaatt cccttcacta tcttggatta atggaattcc atcttttaca
     9541 ccaacaattg tttgtccacc ttctgctttt agtggagttg ttggtgttgg aatatgggtc
     9601 aattcctcta cattactagt gacaatcggt acactcattc cttgagaatt attcgtcgta
     9661 atttcaccgt ttggggttac cgatactgtg acttcttttg ctggaacaat aggtttattt
     9721 tcttttggaa ttgcttctga tttttcagca ggttgtttcg gttcttcttt tggttcttca
     9781 ctggtaaacg acatcgttgg ctgttccgta cttgacgaat cggtattcgt ttcagaagaa
     9841 ctggttgttc ctgattgttc tggagttgtg actatacttg aatccgttgt tgttgaactg
     9901 tgcacacctt cttcttcaat tggatcagtc ggcaaagttg attcaggaat ctctggttct
     9961 ggtgccactg gctctttttc aggtttctct ggctgcaaat gttcaggttc tgctggtttt
    10021 tccgttgttc ctccatttag ctctgtctca acatctgata atccttcccc tggcaatgtt
    10081 tctacaattt ctgacttagg tgtttctaca gtttcatctg cataaaccaa tgaactccct
    10141 aattgaaacg aaaataatga aattcctagt gttgttacga ctgctaatgt tgtttttttc
    10201 atgattattc ctccaatttt ttatgcaatt cgtgttttgt tcatagacgc cgcactaata
    10261 ttatctgaac gataaaagaa ctcccgtccg ttttcttcta acccaacata tggattattt
    10321 ttgaataatt gaatcaatag ttcactggtg ttgtattttg gatagtttgc aaaaaaataa
    10381 tactgtgctt gtcgcccatc cgaaaattca acaagtaatt cataacgata ccgcttcaat
    10441 ccttttcgtt gttttaaagg atctctatac caacttgtcc atcgttccgt tcgtggaccg
    10501 agatagacac gatacacata gaacaatgcc ataccacata ccaccaaaaa taataatcgc
    10561 cacataaaaa ttcccccttt tcttctacct ttcttgaatc accgaacatt cataccctcg
    10621 gtagagatcc gaaaaatcca tcattaattt ttcgtataaa cgttgttgca aatagcgatt
    10681 gtttcgaata cataattctt catagccatc aatcacttga tcttctttct catagcatct
    10741 tgtagctcta tgacgctcag cttgcatgac ataaccaaac tctgcaccat atcgttctac
    10801 cgttaataac tgttgactac gcaataatgc aatataacaa tcacaaaaat gttcagccga
    10861 catttgattt ttctcacgtt tgtgaaatag gtattcttta gaaagcacat tattcgttaa
    10921 acaatctgat aaatagcgat aatctggcgg tcggttattt tcatctgtga atttttccca
    10981 taataaactg atttttattc gttcagcggc tagatagtct tgttcgcgtg gataaaattc
    11041 atcaatcgta caaagctttt ttatcacccg aataaacaaa tccatataat gcgaaacgaa
    11101 ctcttccttt tcaattggct ctcctaaaaa ttccagtgac ataccatctt ctccttcctt
    11161 aaaaaataaa aaagcagaaa ccgatttggc ttctactttt ctttcctttt atagctcttt
    11221 tttacggaag aatccgcaaa tgacattttt tcaaaaaatt aatcggcaca agttcacgaa
    11281 aacttggagc aattttagat agaatcactt ccccatcttg taaaatacaa tcataaaaat
    11341 catcagttac ttgatactta cgcttcttac aataattgat aaaatagtag cgatcatttt
    11401 ctatttccat tcgttgttct actaagcgca tacgaacatc atcatagaaa taagccacaa
    11461 atcgcccttc aagttcacgt ttttcaatgg gatactcttt tggtaacaat ctattttttc
    11521 gtcgtttata cattgctgga taccttagaa gaagttcatc caaacgtctg tcagtatatg
    11581 gcaactgacg aatcaatgat tccaatcgtt ctttatacag atagaataat tggcgcaagc
    11641 aatagtgtac tgcttctgga ttttctaata ctgttggatc acgcgaccaa tacgcaccaa
    11701 cataaatcat aatttttttc atttagacaa tcacaccagc aatactaatc accgatccac
    11761 aataggtaga acaagtggca tagcggccga tttcctctaa ttccagctca atctcttcta
    11821 aagaaagata cgtctgttct tctgttggct ggcgattcat ttcccaagta acagttgcca
    11881 acgagatttc ttccacaaga ggtttttcgt tgctaagtgc tgccacacag ttttcgtata
    11941 tcttcatttt atcgcagcct ttttttaccg ctaaaatcgc aggtaccggc aatgaaaatc
    12001 gataaaagcg aatgaatttt agcgttcctt ttcttcggct aaattccaac tcttcgagtt
    12061 cttgttcgta ccccttacga acttctaata agcgtgcaat ttcttgccag ttttcttttt
    12121 cctgataacg tccagccttt accgcttgtt caatataagg aactcgaaaa cgaaaataag
    12181 acaaatcaac catcttttca tttaatgctt caattgcagc ctttaaatcc aaaagacgca
    12241 gccattctgt tgtcgtatat gcattcatgc aattttccct cctgacgttc ccatccattc
    12301 tagatagctt tgttgctcgt catttagttc agcattctca attccttctt gtacaaagtc
    12361 tcttcctaaa atacgatctg cttcttgtaa taactttaat gtcggagcta cttgtttcaa
    12421 aaaccagcgt tctgaacgat ctaatgagaa tttttctggc tttacggtaa aatccaattc
    12481 ttccatcgaa cgaaacaatt ttgcccatcg ttcatcaaca attcgtgcat cactttccag
    12541 tcgatcccaa acaataagtt ttgcattaat taaaccaacg ccaatctctc ctaaacgttt
    12601 aggggaatga tccaaaataa acgcttctac tgcttgtgtt gctttttcat tagctaggcg
    12661 taactcatat cgattataaa tgccatattt cgttaatgct tctatttctg aaattttctc
    12721 acgattcgca atttcaaaac gtttttcgta gaaattgaaa aacaatggac tctttcgcga
    12781 tccaaaatac aaggataacc cttcgcttcg gaaataacca tcatctaaaa aacaaccgcc
    12841 aatattctga aatttcttaa agtccatttc taataaacct gctttttgtt tttctagtaa
    12901 ttcaaagaga tcaaaatgtt cttctgtttc caatccccaa agttcatcta acgcaatatc
    12961 gaatcgacta cattgtactc gtttataacc agcttccaac tttccttgat ttttccaaat
    13021 ggaatatagc catgttcgcc aatctgttcg ttgttcacgc atgacttcct catattctcg
    13081 gcaaccttga cccgaaaact gtacaagtac attttgtgtt acagtttgtt ttgaatgcca
    13141 gaatacccag atatttccac gaacagaaag acaatcaaaa agatacaagc ccgtcggacg
    13201 tgtttcaaat tctgttagat ccattcgtaa cacacgttga atcactattt tgtagtctgt
    13261 cccaaaaaag gagagacgca aatagtcgat cattgctcga agatctattc cgcttttcat
    13321 atacaatcga aagcgttcct tgattcgttt ttcgaaatcc tctgggattt tcttttttcc
    13381 ttgttccatc ttctttatat aatctaccga gcatcccaat aactgtgcaa aacgtttttg
    13441 agagagtaat tttttccctc cacctgaagc gtgaatagca ctcaattttt ttcgataatt
    13501 tcgcaattca ataggtgtca tattctacct cctttataaa ataacaaatt tcgattttct
    13561 taatctgcta gaaatcccga tataacaggt ggtaccccct aatttttcta aaaaggagta
    13621 ctccttctaa ccttagctaa agccttgata taactgattt aaatacactt ttttatttgt
    13681 gttatgttat ttctgccccc catattatat atcggggggc tttacacaaa gaggccgtgc
    13741 gcaagcgcac gacctctttg tgggcacaca tgccaagcat gtgctgccac gactggtctc
    13801 ggtggcttcg ccgacacaag ccgcggcagc acatactcgg acaatgtgtt ctcccatagt
    13861 ccctatgaaa gccaccccga tatctaaacc aatgaatctt ctttttcttg taattcttta
    13921 tactgttcag gagcaaaaca ctgtaaaatc gctaaacgta actgtatgtt tttctctttg
    13981 cataaccgat atacctgacg aaaacgcaat atatcctctt ttgtcaaaac aatctgattt
    14041 ctcgtaacaa acaatgtctc gcgctcaatt cgtcggaaaa cataagaacc ttttgtgtag
    14101 tattctactt tgttaatcca ctcgtataat cttcgaaccg caaaatcagg tgaaatttca
    14161 cccgcagctg aagaaacacg atacaaaact tcacccatac ttcaccactt ccttctaaat
    14221 tataaaagtg tataactttc ctcatctacc ccatttgttt tcgtatttcc ttttaacgat
    14281 tgcgcttgac gatcaatttg ttcttcgtaa ctagtaaacg cctcttcttt cagtacagaa
    14341 aaatcttgca caatcattgt gtcaaatttt ttaaattcct caataaagtg atagtctttg
    14401 taaacaaatg gcgcaaagaa ttctacaggc acatcaaaac caggaaaagc atacccacga
    14461 ccaggttgat ctttgttata aaacggctta ttcttaaaat cctctccaaa agtcattaaa
    14521 tagccaatgg gagataaacg gccaacagaa atacggaacg ttaaactatc tcgaacacca
    14581 cttggaaaat attccgcatc tggtcgctgc atcgctaaaa taacatacat gcctgcttga
    14641 cgacctttta atactaatgg ctgaattaat ttatcaactt tgtttctcgt ttccatatcc
    14701 aacgaaccat agaaagctgc ccattcatca aaaaagataa aatgcggcgg tatcccataa
    14761 taagcataat ttttccctgg ttcatactta ggctctttca tcgttttcat ataggtatat
    14821 cgctgattca tttcttgtaa cgcatttcgt aagcaaataa tcatttcttc attcgttttg
    14881 taaaacacat gtcctttgaa tacatcaata tcacctaatg ccgctaaatc tgccgctttc
    14941 ccatcgcaaa tatctactgt ccctacatct aaacaagctt tgatcaatga atacaataaa
    15001 taggtttttc cgccaccaat cgcaccagaa attaatagat ttggcgattg atcatatacc
    15061 caatccaatg ttttcattaa atgaatacgt ccattttctg ctatacattg atcaatagga
    15121 atccgaccag aatcggaaag aaatacataa ttgattcggg ctttttctcg ttcttcacga
    15181 attaaatcac gcaaaaatac ttcggataat aagctaccca tttttaaaaa tcgttcgtgg
    15241 aaacggccac catctaaagg aaaagaaacc actaattctt gtttcttttg ataaaaataa
    15301 acttttggta atctcatttt ttccttcgtt tgtttctcac caacttgctt ctttgtataa
    15361 acaaaccgat tgtcaattaa cattcgagca agtaactgac gccaataaac agaagcaaaa
    15421 tatccttcct ttagtcgcga ccaacgcatc aaacctaaaa ttaatccact aaccagcaac
    15481 aaatacatca caatactgat tccaggaata agatacattt gccaattcga atacgtattt
    15541 aaacgaggat aaaatacttt ccaactaaac caacttaata gaatcaaaaa tggtaacaga
    15601 aagaaaccat aataacaaaa ccataaataa cgattgaaat aacgaattcg tcgaccacga
    15661 tacttaaaaa aatgaggttc caattgtcac attcctcctt tctgttttta cttctcctgt
    15721 gctaaaaatt tttgacaaaa agcaactaat actggaatat cacatttaat aaaaaaccat
    15781 aatcgaaaaa gatttaatcg atttttatat gggtgtgcac acatattaca tattcgtact
    15841 acacgaatcc aaaccacttc ctgatggtct tgtttccact gatacggtaa tgaaaaacga
    15901 acaagatcac gtaaaaacat acactgccca ataaccgcac aaatatactc atttgtttgt
    15961 ataaaaatcg ttggattgtc tccaaaatac tcaatcaatt gttggatatt ttgacaacaa
    16021 accataatct ctcgaagtgt ttgattggtt ttgtccagat tcaaaatctc cccttccttc
    16081 taccttaaat agaaagagta ggaagacttc ctcctactct ttaaaaaact tattttttct
    16141 ctttttcctt agaatcagca cttggttgtt tttgatctaa acctttttct tgtgattgtt
    16201 tcggattaga attatttgtt ggagcttcta ctttctctaa tcctttcgca taaatcacta
    16261 aatctgaata ttcacgattg ccatcttgtg ctggaatata taccacatga tattctggat
    16321 caattaattt cacaggatca cgtcgtttaa aaccagtcaa atctacatct gctggaaatt
    16381 taactcgtcg tgcaatcttt ggttcatctg gatttccaaa aactaattgt gctcgctgtg
    16441 atcgaatttc tcccgtataa tcgcctgttt cattatcaaa tacacgatcg gactcttcta
    16501 atgcgccaaa gcgtacggct cctatacaag tttcatctaa tgcttctgta atatcataat
    16561 tatttggaaa ttttaatccc atcttacaaa acctcctcaa tttttacacc agttaaaaag
    16621 acaccgacat cttgatagcc agttgttcga cgctctgttt gtcccatatc atttgtttca
    16681 cgactattca ctctttcttt acgaaaaaca aaataagctt cttcaaatga aatacgtacc
    16741 tctttttctg gtaatcgtaa tcttttatcc tcttcagctg tgagataaac aaaaaaagca
    16801 ggctgttctt ctcgcgaaca ttctgcttgt aattgaacat atcgcccaga cacaccatta
    16861 atttcctttg gtggattagg ttcccgtgaa cccaataaaa atacctgacc taaaaactct
    16921 ttcttcatac gcaatgcttt ttctgccata tcatttttcc tcctcttaaa attcacaatc
    16981 aattctaaac gactctatat tacacaatgg aaagtaatgt ttaacaacaa atggctcatc
    17041 actaggagac atatctacta ttaacatccc gtctcgttca tgcacagaaa tatcagtaca
    17101 ctcctcatac gtacattctt ttccactcaa aaaacaaata tttatatttt tcttttccat
    17161 attctttttt cctccattat tttcttttct gtaaaaacca agtaataatt ccttttccat
    17221 cttccaacac aaactgatga tatattt
//
LOCUS       DACENJ010000038        17154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.038, whole genome shotgun sequence.
ACCESSION   DACENJ010000038 DACENJ010000000
VERSION     DACENJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.038"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(151..2058)
                     /locus_tag="IW748_000952"
     CDS             complement(151..2058)
                     /locus_tag="IW748_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1103874.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2188..3063
                     /locus_tag="IW748_000953"
     CDS             2188..3063
                     /locus_tag="IW748_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1103875.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3143..4117
                     /locus_tag="IW748_000954"
     CDS             3143..4117
                     /locus_tag="IW748_000954"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1103876.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4306..5442
                     /locus_tag="IW748_000955"
     CDS             4306..5442
                     /locus_tag="IW748_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1103877.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5579..5923
                     /locus_tag="IW748_000956"
     CDS             5579..5923
                     /locus_tag="IW748_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1103878.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6194..7480
                     /gene="efmA"
                     /locus_tag="IW748_000957"
     CDS             6194..7480
                     /gene="efmA"
                     /locus_tag="IW748_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1103879.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7618..8217)
                     /locus_tag="IW748_000958"
     CDS             complement(7618..8217)
                     /locus_tag="IW748_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1103880.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8621..9742
                     /gene="mnmA"
                     /locus_tag="IW748_000959"
     CDS             8621..9742
                     /gene="mnmA"
                     /locus_tag="IW748_000959"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1103881.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            9988..11409
                     /gene="arcD"
                     /locus_tag="IW748_000960"
     CDS             9988..11409
                     /gene="arcD"
                     /locus_tag="IW748_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1103882.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11423..12541
                     /locus_tag="IW748_000961"
     CDS             11423..12541
                     /locus_tag="IW748_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1103883.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12601..13893)
                     /locus_tag="IW748_000962"
     CDS             complement(12601..13893)
                     /locus_tag="IW748_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1103884.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            14043..14603
                     /locus_tag="IW748_000963"
     CDS             14043..14603
                     /locus_tag="IW748_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1103885.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            14732..15109
                     /locus_tag="IW748_000964"
     CDS             14732..15109
                     /locus_tag="IW748_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1103886.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15042..17114
                     /locus_tag="IW748_000965"
     CDS             15042..17114
                     /locus_tag="IW748_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1103887.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         5478 a         3107 c         3656 g         4913 t
ORIGIN      
        1 gaaactaagc agctttttca caacctctaa tacatggtat tccagaagta acttcttcga
       61 aaaataagcc gaaatgtcac aaaaatttga gaagcgattt tcccaaattc cttcttattt
      121 cttgaagctg accacttctg gcacaacctc ttaccagatt cgcactcttt tttctggcgc
      181 tagatacata gcgtcttgtt ccgtgatacc aaatgtttca tagaattcgt caaggttttt
      241 tggttgcacg tttgctcgga gtttagcagg ggcatggaca tcgatttgca gtaaaagttg
      301 ttggtattct ttttttgctt ttgtacgcca aatcgttgcc caattgacaa agaattcctc
      361 gatagaaaga tctgattccg tttttgctgc ttccaatgca cagcttagac cgcctgcatc
      421 agcaatattt tccgaaacgg ttaattttcc gtttactttt ccgtcagcaa aaggcagacc
      481 atcgaattct tcgatcattg cttgtgcttt gttttgaaat tgtgtaagat cttcttctgt
      541 ccaccagttg ttcaagttgc catattcgtc aaataaagcg ccgttattat caaatgcatg
      601 ggaaatctca tgagcgataa cagctccgat tccaccataa ttagcagagc ttgattgttc
      661 tagactgtaa aatggtgctt gaaggatcgc tgccggaaag acgatcacat tccgaaatgg
      721 atgataataa gcattgaccg tgtcagcact catttcccat tctgaacgat ccactggttt
      781 gttccatttg ctgaaacgat cttttaatgt gagtttgctg aaaacaagag tatttgaaag
      841 caaagtacca ccctgatctg caggtactgt tttgaactgg tcgaataatg ctgggatctt
      901 atcggggtag cctacttgga tacctaagtt atttaatttt atgacggctt tttcttttgt
      961 tgcatcccca agccagtcat tttcttctag acgtttttta taaacggcga tcattttttt
     1021 caccatttgt tctacgtctt ttttagcagc ttcgccaaaa tatttttttc cgtagtaatc
     1081 gccaactact tgatcgaatt gtccagaggc aaggtaatac gctgcttttt tctgaggcat
     1141 tgcttcgtct gttccagaca atacgcgaga atagattccg ctgacttggc ggaattcttc
     1201 tgatagataa ccactaagag aacgtactgt tttaatgatc atccagcttt tgaataacgg
     1261 aaaatgagct tctgtcagga tgtcattcaa atgatcgaag tacactggat cagttacgat
     1321 cactttatct ggagttgtcc caatcaaacc cgtgacaagt tttttcaagt ccagtttatc
     1381 tgtgtattta ataaatgtat caaaagattg cggattatac attttgctgt aatctgcgtt
     1441 ttcttctgca ctttttacat gaggagcaat caatttatca aattgcactg cttgttccgt
     1501 gattgcttct gcctgacttt tttctttgcc ggcaagctct aataactgaa cagccatatc
     1561 aaagaaaacg cctattagtt gtgcaccgtt gggatggtct gatgcataat acgtcttatc
     1621 tggaaggaat aaagaaggcg gataagcaaa gaaggcgttc gtttgcgcat tcttcatatc
     1681 tgcatctaca tccagattaa atggcaatgg taatgtatcc aacagccatt ctggaaattg
     1741 cttattcaat tcgtcgaaag actgcaatga ttcaactttt tcgattatag gttgaagcgg
     1801 ttgagcatgc aactcatttc ttcggtcata atcgtttgcc agttgataat atgcaaggaa
     1861 atgtttcatt ttttctgaag acactttttc tggatgggca agcagatcgt ctgtttctcc
     1921 catcaaaagt tggtcgatcc cgtcaactaa atcttggaaa ccgcctgtag ctggtttatc
     1981 tgcagggatt tttgctgttt tttgccattc ttcattgact gcttcataga aatcttgttt
     2041 caatcgttct ttgttcattc atcgatcgct ccttttttgt ttcgtgtgcc cattatctta
     2101 caaaatgaag gaattaacaa atagaacata aaaaattgaa aattcgaaca atcaaactga
     2161 tttgtttttt tcaatccttc acttataatg aaaaaaagga ggcgatccca actgaatcaa
     2221 gtagtggata aacctttata tttcaaagta gataaaaaaa ttaaaaaatt agccagcact
     2281 cagcagctgc agtcaagaaa aagtaaattg ctttttttag ctctggtttt tgaagatcaa
     2341 agttatgtga ttatcgatca atcaggacat ccggtagaat attctccagc aaaatatact
     2401 tatcaagagg gtctttcttg taaaaaatgg aagctgatca atgaagagcc aatcgagtta
     2461 tctagatgga tcaacagaaa agaagatatt cccgttttga tcgaagaaaa aaggtcagga
     2521 aaggaactcg caaattgctg ggtgggattg ccagaagaac gttttttgag gtataagaaa
     2581 tgggctaccc cctcaggcta tctttgtggt acttacgcgg cagcagttct ccttgcctat
     2641 tatcaagact atcgtaaggg gtggatgctt ccaaatgaga ttcgcaagaa aaatacggct
     2701 gattcgttgg ttttgacgaa ggcattgcgt agccagatcc aaccactagg acttcctacg
     2761 attccttttc aggttagtac agggatttct agttttttaa agaagaatgg caatcatgag
     2821 cgtgcacgag caactcttct aggttcatgg cagcgagcta caaaacgtat acgtgaaggc
     2881 aaacctgtga tgattggtat tttgaaagta cttggcagta catatggcaa tcattgggtg
     2941 acagcgtacg cttattttga aactgaaaca ggagaacgtt attacaaagt acacgacaac
     3001 tggggcgatt atcataaagt cattcctgcc agttggagta acggaacagt ttcacttcct
     3061 taagaatttg ctgaaattaa cagaaaatct tggaaaaata tgttaaacta tcgaagaata
     3121 gagaagtttt ggggaggact acatgagtaa taagtatcag gatgacacgt tgaagatttt
     3181 tagtttgaat ggcaaccgtc cattagctga aaaaatcgcg aaagtgttcg gaacagaatt
     3241 aggtaaaagc gtcgtgaagc aatttagtga tggcgagatt tccatcaata tcgaagaaag
     3301 tattcgaggg gatcatgtgt atatcgttca atcaacgaat gcacctgtca atgattacta
     3361 tatggaacta ctgatcatga tcgatgcaat gaagcgagca agtgcgaaaa cgatcaatgt
     3421 cgtattgcct tactatgggt atgctaggca agaccgcacg gcgaaacctc atgaaccgat
     3481 cactgcgaaa ttgatcgcta acttgatcga agaagcaggg gcaactcgag tactgacttt
     3541 agatctgcac acggttcaag tacaaggatt ctttgatatt cctgtagata atttatttac
     3601 gatgccgttg tttgctcatt attaccgtca attaggtctg acgggagatg acatcgtagt
     3661 tgtttcgcca aaaaacagcg gtgttcagcg tgcacgtagt ctttcagaat atttgaacag
     3721 cacattagcc atcgtagatc atgcagatga tgcttcaact gagggtggtt atgtgatcgg
     3781 tgatgttcaa ggaaagacat gtatcatggt agatgatatc ttgaacacag gagcaacgct
     3841 tgcacgagca gcaaatgtat taaaagaaaa cggcgcaaaa gaagtatatg cctgtgcgtc
     3901 acatggatta ttgtccgatc atgcgaaaag tattttagac aatgcaccaa tcaaagatat
     3961 ttgtatcact gattctgttt tcacagaaga aaatcgtcat ccagaaaact taacaatcgt
     4021 tacatgttca gaattaatgg gtgaagcggt caaacgcatc catgaaaata caccgatgag
     4081 tccgttattc cgtctggaag aaaaagaatt cgaataaaca aaaaaaagag ggaaagaagt
     4141 tgtgacagaa gtgagcagct tcaaggagct gcgacaagac ttttgtcaca gctccttttt
     4201 tctatgccca tgaaacgtcc tctagacaaa aatgagctct tctttggttt gttcgctatt
     4261 ttatgctaga atgtgcctag caataatttg gaagggatga atgaagtgaa aactatttat
     4321 ctggatcatg cggcaactac ccccatgcat ccacaagtaa ttgaagcgat gacggcaatc
     4381 atgcaagaca cgtttggaaa tccttcaagc atacatggat ttggtcgcca cgcgcatgaa
     4441 aaattggaaa cagcaagaca aatcattgca gacagtctgc aagcaaaacc tcacgagatc
     4501 atctttaaca gtgggggaac agaaggggat aatacagcta tcttagaaac agcgttctcg
     4561 cgtcaaaaag aaggcagaca tctaatcact acagctatcg aacatcctgc tgttcttaat
     4621 accatgaaat atttagagac aaaggggttt gacgttacat atctgcctgt agatgaaaaa
     4681 gggaatctgt cgatcgaaga ggtcaaaaaa gcgttacgag aagatacgat cttggtctct
     4741 gtgatgtacg ggaacaatga aatcggaaat ttgatgccaa tcaaagaaat cggtgaactt
     4801 ttagcggatc atccagctta tttccataca gatgcggtac aagcgtacgg aaaccaaacg
     4861 atccatccag aagaattagg aatcgattta ttaagtatct ctgctcataa aatcaatggg
     4921 ccaaaaggcg tcggtttctt atataaacga gacggagtaa atatccctgc attgctttta
     4981 ggcggggagc aggaagaaaa gcggcgtgca ggtacagaaa atttagcagg gatctggggt
     5041 atggcaaaag cagttgaact gctgtcacct gaagaacgtg aagcacgtaa caacaaatat
     5101 aaaggattcc agcaattgat tcttgataca ttagaagcaa acgaaatccc ttatcaagta
     5161 aatggtgatt taacgaataa actccctcat gtgttgaatc ttcgcttaat tggcgaagca
     5221 aatgatttgc tactgatgaa gcttgatctt aatggggtcg ctatttctac tggttcagct
     5281 tgtacagcag gaaatatcga gccttcacac gtattagaag caatgtatgg agaagataca
     5341 ccgatactaa aagaatccat ccgtatcagc tttggtttgg gaaataccga agacgacatc
     5401 cagtatttcc ttgatcaatt agttcgtgct gtcaaaaaat aactagtgaa gtcattctta
     5461 tatgaacaga tgtctgagag aacgccttca ttttttaggg aacttaactg aaaaacctag
     5521 taaaaatcag gataaacttt tataataaag agaaactaat tttatcgagg tgaaaatcat
     5581 ggcatttgaa acaacagcaa cggtactggg cgcaccaatt agctatcgtt tgcatcctga
     5641 tgcaaaaaga tatacattgc gcgataatgg atttacagaa acaaacggag gcaattatca
     5701 attgatccga ccattagatg ccacgccgca aagcaaagaa ggattcaaat taaaaatcac
     5761 tgtatcaaaa gatattcaaa cattgaaaat gtcgatcact acagcaaatg gattgaaagc
     5821 agtcaatatt ttcaaagatc caaaacagaa aatgagccaa gataaatttt attttctgat
     5881 ggatgggatg attagccgtg ggttattcga aaaagtcgaa tgatctgtgg atataaaaag
     5941 acaactagtt ttgaaacggc ctattgttag atgactaact ttgtaaggac gacgcaaatt
     6001 agttgtcttt tttattttta aatttaacac atgttgttat ttttaatatt ttgctgattt
     6061 attgtttcta agtaaagaaa tttaaattga aacgattgtt atttagggaa aaaggtattt
     6121 tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg ctatacaaaa tgaaaaagaa
     6181 aagagggatg tttatggaaa atgaacagtc ggtcgtttta acgaattgga agagaaatta
     6241 tttgttcttt ttatcgggac agtttttgtc aggaatcact agtatggtcg tccaatatgc
     6301 gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca attttgagtt ttgctacttt
     6361 actaggaatg attcccatgg ttttactaag cccatttgta ggaccgctag tggatcgctg
     6421 ggataaaaaa gctcttttga ttgtcacgga tattattgta gcgattttcg cgcttatttt
     6481 agcagtagtc ggaacgattt cagaatcctt cccgatttgg ctagtttttg tgtctttgtt
     6541 tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc cagtcgatta tgccgacaat
     6601 cgttccatct tctcacataa caaggacgaa tggacagtta ggaatggtcc aatcagcgaa
     6661 tttcatcatt gcgccagctc taggtgcggc tttgttttca gtcgttcctg tcaattacct
     6721 tattttatta gatgtattgg gggcagtttt tggtgtcggc ttattgattt ttgtgaagat
     6781 cccaaaagtt tctcctgaaa tattggaagt tccgcttact atttttaaag atgcaaaatt
     6841 tgggctgcag caattgatgg ataataaagg gttatggtac ataacgatca atggtgcatt
     6901 tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca ttgatgacgc tagattactt
     6961 tggtggttcc gttggacagg cgggagcagt ggaagtagtc tacgcagtcg gaatgctctt
     7021 aggcggtgca ctgatcagtt ttataggaac atggaaagac cgaatgaaac ctattattat
     7081 agcctacatc attatgggcc tgacgatcgg tgcaagcgga ttggttccaa acgatagtca
     7141 agggttcttg tactttctta tcttgaatgc aggagcaggt tgtgcaacgc catatttcaa
     7201 tacactgctg atggcaatga tccagcagag ttatgaatcg aatgttttag gtcgtgtctt
     7261 agggaacttc aactctttga tgaacctggc aggtccaatt ggattattat ttgcaggacc
     7321 attagctgat cgtttagggg tagaaaaaat gttcttgttt tccggaatcg ggattttatt
     7381 atgcggaatc gttttatttt taacgtcagc tgcccgaaat tatgataaag aattacagaa
     7441 aaaactggta aaagaacacc atgagcaaaa agacgaatag tatggaatgg agttgtgaca
     7501 gaagtcttgt tacaactcca tttctgatcc taatgttcct gtggcaataa gcctactcaa
     7561 atgaaaaata tgagtaatta tctttatttg tttaatactt gatatagggc agttttttca
     7621 cgacctatcg tacttcctct ttgcttcata gatttctttg atttgttgct cagataacgg
     7681 agtgccaaat ggtagttttt cctttaagag ttctctttcc ggtgcgtcta cgcctacatt
     7741 gacatcgttc tcgataggta cgctgtaatg atggatatgt ccatgaagat tgcggatagt
     7801 gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa tattgttgat ggtcgaattt
     7861 aacgatggca cctacatcat aaaaataaaa tttgggactt ccgtcagtaa gcctaggatc
     7921 atttttttct aaataacgga aaaaagcgcg actgtcatgg ttccctttga tcaagtggat
     7981 tgttccttgc agttgagtga ccagttccaa aatttctgca tttgcttttt catattgcgg
     8041 atgcatagca aggtcgccta gatgataaac atggtcggtt tcttttatta ctgcattcca
     8101 gctagtgatc aactgttggt tcatttcatc cacatttgca aagagccgcg gagcaaagtc
     8161 gttttttcct aataagtgtt catggaaaaa gtgcatatca ctgataaagt aattcatgta
     8221 tcttttgcct cccttttttc ttagagtaaa caaaatcact tatctgagca actcataaca
     8281 tctaaaaaga aaaggttgta aatgagacgt gcagctttaa gcgataagaa gcaattttga
     8341 aaaatagttt ttcacaattt ataaaattat tccttattgc cgaaaggctg gcccttttta
     8401 tgagtatgcc aattttttat atcgaatgct caaaagatga gtgatttttt ggttgactat
     8461 catgtttgtg tactgaatag gtccagtaga acagtatttt tacgacttga taattaaggg
     8521 atgaaatttt cagaaatatg acgtataatg taaaatactg aaaagttgcg accttcaact
     8581 cgtagatgtt tcagaatcta attgaaatga tggtgatatc atgacagaca acagcaaaac
     8641 acgcgttgtc gtagggatga gcggcggtgt cgactcatct gtaacagcac ttttgttaaa
     8701 ggaacaagga tacgacgtga tcggaatatt catgaaaaat tgggatgaca cggacgaaaa
     8761 cggtgtatgc acggccacgg aagattataa ggatgtagcg aaagtagctg ctcaaatcgg
     8821 gattccttat tactctgtga attttgaaaa agaatattgg gatcgtgtat ttgaatattt
     8881 cctagccgaa taccgcgcag gacgtacacc taacccagac gtgatgtgca acaaagaaat
     8941 caaattcaaa gcattcttgg attatgcaat ggatcttggc gccgattatg tagctactgg
     9001 acattatgcg caagtcactc gcgatgaaaa cggcgtggtc cacatgcttc gtggagtaga
     9061 caataataaa gatcaaactt acttccttag ccaattatca caagaacagc tttcaaaaac
     9121 gatgttccct ctaggcggca tggaaaaatc agaagtacgg gcaattgctg aacgcgcagg
     9181 ccttgcgact gctaagaaaa aagattcaac gggaatctgc tttattggtg aaaagaactt
     9241 caaacaattt ttgagcaact atctgccagc aaaaaaagga aatatggtca ctcttgatgg
     9301 tgaagtcaaa ggacaacatg ctggcttgat gtattatacg attggtcaac gtcaaggact
     9361 agggatcggt ggcggaggag attcgcaaga accctggttt gttgtaggaa aagatctagc
     9421 gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct gcattgtatg ccacaagctt
     9481 ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg ccaaaagaat tcaagtgtac
     9541 agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca gttcgcttat tggatgataa
     9601 tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc actcctggac aagcagttgt
     9661 attttatgat ggaatggaat gtctgggtgg tggtttgatc gaccacgcat atcaagaaac
     9721 aaaagtttta caatacgttt aagcgatatt aaatgaatca aaacagctat ggctaacaaa
     9781 agaaacacct tttcttttgg atagcatagc tgtttttttg caataaaatt aatttttatt
     9841 cattttatac ttgaaatact tgtgtaaaaa gttcataata tccaataata tgaataaagt
     9901 tgctttttgt taagcaagag cagttgcaaa atagaaggga atcttttgca agtgatttgt
     9961 tcaattttat aagtgagggt gatggaaatg gaaaagaaat cgacgggtat tagcaaatgg
    10021 ggactggttg ctctggttgt cagttcgtca atcggaagcg gtgtgtttgg gatcacaagt
    10081 gatctggcta gttctgcagc tccagggcca gcgatccttt catggataat cgttgggatt
    10141 ggtattttag ctttggtatt atcgctgaat catttaggag aaaagcgtcc agatctagat
    10201 ggtggtattt tcggttacgc ggaagcttca tttggtaaat taggtggctt catcagtggt
    10261 tggggttact ggttgtctgc gtggctggga aatgttgctt ttgcaacgat gctgatgagc
    10321 gccatgggag aatttttccc gatattcaaa ggtggacaaa atgttccttc tattttattt
    10381 gcaagtattt ttatttgggg tctgacttta ttagtaaaca atggagttga aggagcgagc
    10441 gtcatcaata caatcgtgac gatctgtaaa ttagtgccat tattcctatt tttaatattt
    10501 gtatttatcg catttaaagt gaatatattc actgctgatt tttggggtaa cgtatctgat
    10561 aatctagtga atggaacagg gcagcaagga acgatctggt tgcagattaa aggttgtttg
    10621 atggttatga tgtgggtctt tgtagggatc gaaggcgcaa gtgttttagc caaccgcgcg
    10681 aagaaacgtt cagaagccca gcaagcatcg atcattggtc tgctttgttt attgtttatc
    10741 tatatccttg cttcgatctt gccatacggc gtcttatctc aagaagagtt agcaactatt
    10801 tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg gaaattgggg agcagcgctg
    10861 atcaatatcg gcttgattat ttcgatcatt ggttcttggt tgtcttggac gatgctccca
    10921 gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc cgaaaggttg gggaaaaacg
    10981 aataaaaaaa atgccccaac ttattcgtta gttgttactg caatcttgac taatctattc
    11041 ttattgactt tcttagttac tgattatgcc tatcaatttg cctattcttt atgtacagcg
    11101 gcaattttga tttgttatct acttgtcggg atctatcaag tccagttttc tttaaaagaa
    11161 ggaatcaaaa aacaagtagt gatcggtggg attgctgtag tttttgaatt gatgggcatc
    11221 attttagcag gattttctta tgtcctttta tgtagtattg cctatcttcc tggtttttgc
    11281 ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta cgggaaaaga aaaagtaatg
    11341 attgggctga tttgtgtggc cgcactgctt tcaatcgggt tgcttgctgt aggctggatc
    11401 aagatataag ggggaaagaa acatgaaaat cgcaaatttc ggtgtagaag aatggctaaa
    11461 taaatgggaa aagaaagcgg tatatgatat tgcacaaagc tcgatcgaag cattgacatt
    11521 agacgaactc gttggcttag atgggactag cgtaagtaaa tttttcgaaa aacgcaaaaa
    11581 cgaaccattg gactatggct ggatcgaagg atcagatgtt ttcaaacaag aagtcgcttc
    11641 tctttatcaa accgtcgatc cggaaaacat cttacaaacg aatggagcga ctggtgctaa
    11701 tcttttagct ctatacgcgt tagtggaagc aggagaccat gttgtttcga tgcttcctac
    11761 ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc gctgttgact ttgttcattt
    11821 gaaggaagaa gaagattggc agtttgattt agaacagcta gaagccttag tcaaacctga
    11881 aacaaaaatc atctgtttga atagtgcaaa caatcctaca ggcacattgc tagatcggaa
    11941 aactttagaa aaaatcgcag aaattgctcg tacagtagat gcatatgtat tgatagatga
    12001 agtatatgcg cctttaacgg ataaaggtga attcttgtcg atcgtagatg tctatgaaaa
    12061 aggtatagca accaattccc tatccaaaac ctactccatt ccaggtattc gtattggctg
    12121 gacagctact ggacctgagt tggcagaagt ttttcgaaag tatcgagact acacgatgat
    12181 ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca ttgaagaata aagaaaaaat
    12241 attggcgcga aacaaaaaga tcattacaga gaaccttgtg attttaaaac aatgggtagc
    12301 caatgaaccg aaagttgaac ttgtcgctcc caattacgtc tctacttctt tcattaaatt
    12361 agccatcgaa gaagatgacc agacattttg tatcaattta ttagaagaaa caggagtact
    12421 attagtacca ggaagtgcct ttgatttacc gaagcatgcc cgactaggtt actgttgtaa
    12481 aaaagaaacc ttggaaaagg gattattcct cttatctgag ttcttgaaaa aacaggccta
    12541 aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa gcagtgtttt tcatttacgc
    12601 ttattgtcta tgagaaggag agtgccctga agctatggag actcctgaag ctttttcttc
    12661 tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt acaagcaaga agttgaccaa
    12721 aacaagaaga gaagtcgaaa gaaagacgcc acgataacca aatgcactgg aaacactgga
    12781 gccgatcata ggccctacta cgttaccagt tgcttggaaa gattggttat aactgaagat
    12841 ccgtccagca gcttgatgag gcgaatattt tgtgatcaat gcttggacag cagggagcag
    12901 acaagcatca gagataccaa tcaaaaaacg taaggcagct aattgccaaa cattcgtgac
    12961 gaatgccatt gggatataaa ccagaatcgc aaaacccaaa ccaatcgcaa gtattctttc
    13021 actcccaatc ttgtcaccta agcgaccaaa tcttggagcg gctatcaaag tggcaatccc
    13081 aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc ccatgtccat gcagcaattg
    13141 gcgaatgtac agactgatga ttggactaat ggaattgttg gatgcttgga tgatcatcgt
    13201 tgtgacgaac ataccgatga cgacgtgtgg atatttcaag tctttgaaaa tctgtttggc
    13261 agaaaccatg ttttcttttt tcactgggac aaatttttca tggacgaaaa acagactcaa
    13321 caagaagacg aaaaataaga tgatccctgt aatgaaaaag gtagggcgat agccaaacgc
    13381 tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt cctgtaacag aaccagttgc
    13441 taaggttcca agaacttgac cacttttttc ttttggcgtc ccagttgcga caagggcagt
    13501 ggcattactg atataaccag aaaaaatccc ttgaagcaga cgcaatgcga ctaattgata
    13561 cacactagtc acgcaaccca tcaaagaaat gacgattgcc attcctaatg aagcacgcaa
    13621 cagcatcaat tttcttcctt tttggtcggc taattttccc caccaaggag agataagcgt
    13681 agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa aagttcaatt gtgacgtact
    13741 ataattacct aacgtatcta tgtaaagtga cataaaaggc atgactaaac taaatccgat
    13801 accagccata aatgttccaa accataagac aaacaagttt tgttcccaag tttctctttt
    13861 cctaaataat ttttccttta ttcttgcagc caattagact ccttctttct gaaaattttc
    13921 atctacagct ctttattata aactacaata tgtagtttat caatgaaagt ctcttttttc
    13981 tctttctttt tcgtggtaaa ctgaaagagt gaaggattta ttttatgaga ggaaacatgg
    14041 aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga ctgctttcga gaactagcag
    14101 aagagatgga tgttcagcaa ataacctttc agcatttagc caaacgcttg gatatcaagg
    14161 ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt caaaacagcg ctaacagcga
    14221 aacttctgca ggaacttaat gaccagctgc gacgggctct tgtgggaaaa agtggtcagg
    14281 aagcactgca gatctatgca gaaacttatc gatcctttgc tttttcaaat caagcagtgt
    14341 atgagttact gatcagtgta ccgcatacca atgaagctgt tttattagat ggaatccatg
    14401 aaacgaatca gatcattttg cagatatttg aggcttttca attaacgaaa gaagaaaaag
    14461 ttcatcgaag cagggaactt cgtagtatga tccacggtta tctttcctta cgttttttag
    14521 gatactttac aaaagaacca acaatcgatc cagaagacag ctatttttgg atgatcaata
    14581 actttatcgc tactttgcct taaacaatca gattcatttg taacaatttt aagatgttca
    14641 ttaaatgacc cttttataag agaaaattct tgcaactttc ttgaaaagtg actaagatga
    14701 gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa gtaattacca tgttcgggga
    14761 caatgagcct tggtggtttt ttgaagactg ggaagaagat attgaagaag aagaaacatt
    14821 cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa tggaacgaaa tccatcaaaa
    14881 ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc ttttggaatg acggagatga
    14941 gcgctggtgt gaagagtgtg acgatgattt acagcaatac aagggacttg cgttgttgaa
    15001 aaatcatcag cccatcacag tagaaagcag aaaggaattt tatgaaacaa ctaattctag
    15061 cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct tggtgctaat caaaagcaca
    15121 aaaattatta tgaaggaccc aaggtgatcg ttacttgggc ccttggtcat ttattaggat
    15181 tgaaaatgcc ggaagatctg aataaggaat ggcagagctg gcaaatggac acgttgccaa
    15241 tgatccctaa aaaactagga atcaaaccac ttcctaaaac aggacatcaa ttaaaagcaa
    15301 tcaagcagct ggctcagcga aaagatgtct cagaagcagt cattgcgaca gatgccggaa
    15361 gagaagggga acttgttgct cgctggatct tagagtatgt gcatttcaat aaaccagtga
    15421 agcggttatg gatctcttct caaacagata aagcaatcaa aactggattc aagcaactaa
    15481 gacctgcgaa agaatatgat gcattgtatg aatctgctct tgcccgggca aaagcggact
    15541 ggctggtagg attgaatgtg accagagcct taactgtgaa gtacgcggat aatttatcag
    15601 caggccgtgt gcagacaccg acactagcat tagtgagaga ccaagaaaag aaaatcgagc
    15661 agttccgtcc acaaacgtat ttcacgatca cgttgcaagc tggaaaagaa caagccaaaa
    15721 tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga agcacaaaaa cttgttcaat
    15781 tgatcagtac aagcaaagga actgtcacag aagtccaaga aaaattaaaa acggaatctg
    15841 cgccattgcc ttatgattta acagagatcc aaagagaagc caatcaacgc tatggctatt
    15901 ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga gacccataag atcgttactt
    15961 atccgcgtac agacagcaaa tatctgacaa atgacatgaa aagtacgatg aaagaacgat
    16021 tgcaagcagt cagcgacttt gctcctgagg tcaagcagta tttgaaaaac ggagccatcg
    16081 tccaacagac aaaagtattc caagacagta aagtgaccga tcaccatgca cttttgccaa
    16141 ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga gcgaaaaatc tatcaaatga
    16201 ttgtctctcg ttttttagga ctgtttgctc aaccccataa agtcagccaa acaaaagtta
    16261 cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa tcgtgtcgtc caagcaggat
    16321 ggaaaggcga aactgaaact gagacggaaa cagtcaaatg ggaaaaagga atgagaatca
    16381 atcccgattt cgccatcaaa aaagaactat ctgcaccgcc taaaccattg tcagaagcaa
    16441 gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc agctacgcgt gcagagatta
    16501 tcgagaaact aatcaaatct gaactgatgg aacgtacccc tcaaggactg caagtatctc
    16561 ctaaaggtaa gcaattatta gtattggtaa atccctcttt agtaacacca gatttgacag
    16621 aaagctggga aaaagacctt gaagctattg ctgcaggaaa gaaacaagca ggaaccttct
    16681 tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga gatcaaatca agcaaacaag
    16741 aatatcaaga tttctcgatt acccaaaaga aatgcccaga atgtggggcc aatctgcgcg
    16801 agaaaaacac acgtgacggc aaaatctatg tctgcaccaa ttcagattgc aactaccgca
    16861 gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca atgtcaccgg aaaatggaaa
    16921 tcatcgaagg taaaaacggt gcttatttcc gttgtaaata cgatggaacg acagaaaaat
    16981 tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca tgaagaacga cgcttgctga
    17041 aaaaatatgc acaagcagaa gaaccagaag aaagcccatt agcagcagca ctaaaagcag
    17101 caatgaagaa gtaataagat atgaaagagg ttgtgataga agtggtcagc ttca
//
LOCUS       DACENJ010000039        17107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.039, whole genome shotgun sequence.
ACCESSION   DACENJ010000039 DACENJ010000000
VERSION     DACENJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.039"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..588)
                     /locus_tag="IW748_000966"
     CDS             complement(43..588)
                     /locus_tag="IW748_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1103888.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(600..1292)
                     /locus_tag="IW748_000967"
     CDS             complement(600..1292)
                     /locus_tag="IW748_000967"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1103889.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1311..1595)
                     /locus_tag="IW748_000968"
     CDS             complement(1311..1595)
                     /locus_tag="IW748_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103890.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1608..2171)
                     /locus_tag="IW748_000969"
     CDS             complement(1608..2171)
                     /locus_tag="IW748_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1103891.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2399..3061
                     /locus_tag="IW748_000970"
     CDS             2399..3061
                     /locus_tag="IW748_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1103892.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3185..4381
                     /locus_tag="IW748_000971"
     CDS             3185..4381
                     /locus_tag="IW748_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1103893.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4482..5858)
                     /locus_tag="IW748_000972"
     CDS             complement(4482..5858)
                     /locus_tag="IW748_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1103894.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5895..6761)
                     /locus_tag="IW748_000973"
     CDS             complement(5895..6761)
                     /locus_tag="IW748_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1103895.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6850..7875)
                     /locus_tag="IW748_000974"
     CDS             complement(6850..7875)
                     /locus_tag="IW748_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1103896.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7893..8567)
                     /locus_tag="IW748_000975"
     CDS             complement(7893..8567)
                     /locus_tag="IW748_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1103897.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8751..9644)
                     /locus_tag="IW748_000976"
     CDS             complement(8751..9644)
                     /locus_tag="IW748_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1103898.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9718..11415)
                     /locus_tag="IW748_000977"
     CDS             complement(9718..11415)
                     /locus_tag="IW748_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1103899.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11408..12721)
                     /locus_tag="IW748_000978"
     CDS             complement(11408..12721)
                     /locus_tag="IW748_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1103900.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12721..16239)
                     /locus_tag="IW748_000979"
     CDS             complement(12721..16239)
                     /locus_tag="IW748_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1103901.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16532..16990)
                     /locus_tag="IW748_000980"
     CDS             complement(16532..16990)
                     /locus_tag="IW748_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1103902.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4815 a         3845 c         2957 g         5490 t
ORIGIN      
        1 tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa tttctgcgcc
       61 tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga aacttgccac
      121 ggcatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg ctgtatgcaa
      181 cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc gttctcgcaa
      241 acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta tccaataaac
      301 attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca attcccgtcc
      361 acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa aggaatcttt
      421 tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa acttctttgt
      481 atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca gctttccatc
      541 ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct tagtcctcct
      601 tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc tgcgtcttcg
      661 ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat tgcacgtacg
      721 atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc ttccatttcg
      781 caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg cgagtcgaca
      841 aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc ttttgctgct
      901 ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat tcctggtaat
      961 tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac tttgtctgat
     1021 ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc tgtattgatc
     1081 accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc taagactttt
     1141 cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga aataaataaa
     1201 gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat ttttacttct
     1261 tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt ctatataaat
     1321 gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga tttcatcaaa
     1381 aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt gattggttta
     1441 ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg ctgataagct
     1501 gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg tcgcttgcga
     1561 agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta ctttcctttg
     1621 gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat caactgatct
     1681 ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac ttcgtcttca
     1741 tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat atgaaggact
     1801 tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga cttcgcacga
     1861 tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg tccttctcct
     1921 ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt tcgaaattgt
     1981 ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc gccaggatga
     2041 aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc tacagcaaca
     2101 tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc aaattgctca
     2161 ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt ttcatttttc
     2221 aatttttctc aaaaactcct tattagttta ccatagataa accttagatt ttctctaaat
     2281 atagaaaatc cttctttagt ccgatactct ttatagtagt ccgtatgcta aaataagtat
     2341 gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag ggtttattat
     2401 gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct ttgtccgaaa
     2461 caatagtttt tcttttgttc taggactttt attgataggt gtagttttag ttttgtgggg
     2521 actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac tgactgacga
     2581 acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat tttttcgaca
     2641 aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc agcagacagc
     2701 caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag ctttattcaa
     2761 agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt atacgcatct
     2821 gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg agatcaaaaa
     2881 caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc tgtcagcttt
     2941 aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg acgtagagat
     3001 atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac aaaaggatta
     3061 actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct gtttcgagta
     3121 ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca aggaggaatt
     3181 ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg atacacttga
     3241 agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt cacaacaagc
     3301 ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca aactaccggc
     3361 ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg atgcacagtc
     3421 tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac aatcgatgtt
     3481 gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg aactgatgta
     3541 tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca tcttccagcg
     3601 tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc aaaagattgg
     3661 tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc tgctaaaaga
     3721 taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg cactgaatat
     3781 ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca ttccagaagc
     3841 gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca atgattacac
     3901 gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta gacagatcac
     3961 gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct tggctgaaaa
     4021 gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg tgatcgcatt
     4081 gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg aaacaacaaa
     4141 cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa cagcgaaaga
     4201 aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg atttgatcga
     4261 aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc gtcatgcaga
     4321 agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt taacccaata
     4381 gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac aactgttgat
     4441 gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc attcatcttg
     4501 ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac gataccgaac
     4561 acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa gatggagacg
     4621 attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt gccaaacatt
     4681 tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc catgatattc
     4741 gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc tgccacacca
     4801 gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt cataaactta
     4861 ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata catcatcgag
     4921 atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc cactgttcca
     4981 tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc agtatacgga
     5041 acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat cagtatcgaa
     5101 ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc tgccattgca
     5161 acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag cacaccggaa
     5221 aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat tcctacaatg
     5281 ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa acgtaatgct
     5341 ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac cgcaatcaag
     5401 tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg aagttcggcc
     5461 cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac agaggaaacc
     5521 gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa ttcaccaaat
     5581 acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc aaattctgcg
     5641 aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag tgacagtgct
     5701 ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt accgatgatc
     5761 gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat ctcactcgta
     5821 tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc ccattttagg
     5881 ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc taaaatgact
     5941 aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc gccaaggatg
     6001 aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa gccaacagct
     6061 aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca taataatcca
     6121 cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc aaagaagctt
     6181 gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc caaattcgcc
     6241 cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa tgctctaaaa
     6301 cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc atcttgtcgg
     6361 gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta tacataatct
     6421 ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa aattaattga
     6481 aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc atggaactgt
     6541 ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa aatccaaggg
     6601 gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc accgatcgtc
     6661 attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa accaatacta
     6721 ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct ccttttattt
     6781 cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt tttcgtttct
     6841 aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata aagtcattgt
     6901 tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt cggccagatt
     6961 ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac catttctccc
     7021 agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga tccctgaatc
     7081 tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga tgtttccgtg
     7141 atccggcgta tatttgatgg cattactaag caattggttg aagataaaag cgatccattt
     7201 agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat ttttttggat
     7261 aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc catattcttg
     7321 gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt gttctacgta
     7381 ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt caatatcgaa
     7441 ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt catggaccca
     7501 tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca ttacttcttg
     7561 gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt ctgtcctccc
     7621 accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt ttccccacca
     7681 tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc cctcaatcaa
     7741 agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc acatcacaaa
     7801 gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat ctttcaaata
     7861 tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc ctgtcctact
     7921 tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa ccgattgatg
     7981 ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc tcgcagcaat
     8041 ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag acgatattca
     8101 tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc aacattcaac
     8161 gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta agaacggcga
     8221 agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt cacaaaatca
     8281 tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct ggagataaaa
     8341 atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc atccaaaacg
     8401 ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc tgagaacact
     8461 tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc ctccatgatc
     8521 aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa aaatccctcc
     8581 atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt ggcttacctg
     8641 gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta atcgcgtttg
     8701 tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta ttatctgtta
     8761 tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc gaaaagcgtt
     8821 agcactagca atatacctag aaggagtata agtaatcgat aaagatggta tttttttgtc
     8881 tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt cactagtaat
     8941 cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc ctgcttttct
     9001 ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac gacttcataa
     9061 gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat ttcttcaaga
     9121 tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga atgtgtactc
     9181 tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc taacaaaaga
     9241 tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata gatggctccg
     9301 atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac cgcttgctca
     9361 aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag cgtctgattt
     9421 ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg ttcaagctgc
     9481 ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc tgctaaaaat
     9541 ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa ttgtaataaa
     9601 agactgattt gggtttgagg tctattacct attttctttt tcattttctt caccttctta
     9661 tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt ttgttgctta
     9721 tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca tcaaacttat
     9781 tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg acccgcgttt
     9841 catattcggt actctttctg gccctggatt ttctactcct acgatctgaa tttcttcatt
     9901 atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg gaagtacata
     9961 accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc cttgaacctc
    10021 aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg cccactcaat
    10081 tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa atattgcgta
    10141 ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt cactgcttga
    10201 tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg gctctactcc
    10261 ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt tgttatggaa
    10321 atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta aatctcctcc
    10381 acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt cttctgtcaa
    10441 aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc cttttgttaa
    10501 aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac caactgtagg
    10561 cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga cagaatcgat
    10621 gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt ctagatcaat
    10681 ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc taaggtcgct
    10741 ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat acaaatgcac
    10801 atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca ccatatcagg
    10861 catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat gagctggcga
    10921 ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt ccgtcacttg
    10981 gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa tacttgtcag
    11041 tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa tcgcttcggc
    11101 agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca aatcgtaata
    11161 tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt caccaaaatc
    11221 cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac cttttttgtg
    11281 taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga ctagttttcc
    11341 gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc ccgtgacaat
    11401 cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca gcacctaaca
    11461 ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc gggaatacgg
    11521 accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt ttgtccgttg
    11581 tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca gagactggaa
    11641 ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat tgtggttggc
    11701 cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt gcttcatcca
    11761 tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa ggcaagaaac
    11821 gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca tttttgtcat
    11881 tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta gcaggagcca
    11941 tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca gcaccttcta
    12001 atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag tatggatctc
    12061 ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg gttgattctg
    12121 tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg atatcaacat
    12181 ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca tcccgatctt
    12241 ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca accgtatctt
    12301 tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt agctgacgga
    12361 tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc atgttttcag
    12421 cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc gctccattta
    12481 gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag atagccaagc
    12541 catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc atccgtttat
    12601 gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca tgtggaagaa
    12661 ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac agctttttca
    12721 ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg ctcctgcaag
    12781 cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc ctgtagcagg
    12841 caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat ttgtctccaa
    12901 aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt ttggataata
    12961 cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat cattttctat
    13021 gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt ttctatagct
    13081 gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg aagcattgaa
    13141 tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat actctcctgc
    13201 actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat tcatactgtc
    13261 aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta cttgtagtac
    13321 accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga tgaccgtatc
    13381 ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat cagatagccg
    13441 atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca gatgcagagg
    13501 ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat agaagtatcc
    13561 gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt atgctcgata
    13621 cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat ctaatgtatg
    13681 gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat tgtaagcagt
    13741 gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt gccaattttc
    13801 ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc gcaagatcca
    13861 tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa tgattttgta
    13921 ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg gctctccgat
    13981 tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat ctactgaata
    14041 gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc ctttcgctac
    14101 tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact ctgcgccatt
    14161 gattggcata ttactgcctt caattagttt attgatattc atgtggattg gcttgaattc
    14221 atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta tgttaggctc
    14281 ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat ctttaccttc
    14341 cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt gacctaacat
    14401 ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat ctactaactg
    14461 ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg ctccagtttc
    14521 atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg tagttttact
    14581 tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg caaattcgac
    14641 tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac ggcctttatg
    14701 ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat cattctcata
    14761 atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc cgtcttgagc
    14821 tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac ctaaagaaat
    14881 cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag tcgccactac
    14941 ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt ggtacttcat
    15001 gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc cagctccaaa
    15061 tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg ttctcttgaa
    15121 ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg cattgtaata
    15181 tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat ttcgtccaat
    15241 agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa tggagaagat
    15301 acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa ctgtgttgat
    15361 cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag aagaaccatc
    15421 accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac gatagtgtac
    15481 atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat gagtaaaccc
    15541 tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt cattatcttc
    15601 agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc tcgtacgata
    15661 acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa cgattaggat
    15721 tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat agccattttt
    15781 aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg tattctcact
    15841 tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga tttgctcatg
    15901 gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa tatccagact
    15961 cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg cagcattttt
    16021 agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat ctagaacaaa
    16081 aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta gatagacatt
    16141 gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg gcagaacagg
    16201 ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt caaaattccg
    16261 ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt cgcctgcagt
    16321 tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt ttgtataatc
    16381 actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt gatagcgttc
    16441 attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa acttcatcta
    16501 ttctttcatc aaccaccact tgaagaggta gttacccttc gttcacatcg gggatctttt
    16561 ctgtttcaaa aatcaatgtg ccggtttctg cttcttttga ataaactttt ccgacatacc
    16621 attcttgacc atcgatcgtc tcgactttta ggtcttctcc atcttcatca taggaggata
    16681 gacctgttcc acgaacgttt tttacggtac caatcccggc ggctgccgca tcgattgcta
    16741 aacgtattct accttcattg gcactttcct ttttttcaaa agccagatgc cattcaaggg
    16801 tttcttcttt ttcaatatac ttcccttcaa tttttgcata gtcctcatct aagaatacct
    16861 catcctcctt tgtttctgtt gactcagcag ctacatccag ggtcgtcata tgatagactg
    16921 actggaaata tggtccaaat agcaaactga cagttactaa ctgcaaaagc ttcaatttta
    16981 accctttcac atccttatac tcctttcaaa aatatttgtc ttctttcaca acaatcaaca
    17041 aaacaatgcc catctaatta taatttaatt taattttaat tttaaatcaa atgatatcct
    17101 ttttagt
//
LOCUS       DACENJ010000040        17083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.040, whole genome shotgun sequence.
ACCESSION   DACENJ010000040 DACENJ010000000
VERSION     DACENJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.040"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..325
                     /gene="sgrA"
                     /locus_tag="IW748_000981"
     CDS             <1..325
                     /gene="sgrA"
                     /locus_tag="IW748_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1103903.1"
                     /translation="SSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSV
                     LKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSL
                     FHRVK"
     gene            623..979
                     /locus_tag="IW748_000982"
     CDS             623..979
                     /locus_tag="IW748_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103904.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            982..2139
                     /locus_tag="IW748_000983"
                     /pseudo
     CDS             982..2139
                     /locus_tag="IW748_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2165..2605)
                     /locus_tag="IW748_000984"
     CDS             complement(2165..2605)
                     /locus_tag="IW748_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1103905.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2765..3271
                     /locus_tag="IW748_000985"
     CDS             2765..3271
                     /locus_tag="IW748_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1103906.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3439..5352
                     /locus_tag="IW748_000986"
     CDS             3439..5352
                     /locus_tag="IW748_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1103907.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5357..6358
                     /locus_tag="IW748_000987"
     CDS             5357..6358
                     /locus_tag="IW748_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1103908.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIEKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6560..6766)
                     /locus_tag="IW748_000988"
     CDS             complement(6560..6766)
                     /locus_tag="IW748_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1103909.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(7412..8260)
                     /locus_tag="IW748_000989"
     CDS             complement(7412..8260)
                     /locus_tag="IW748_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1103910.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            8424..9326
                     /gene="gnd"
                     /locus_tag="IW748_000990"
     CDS             8424..9326
                     /gene="gnd"
                     /locus_tag="IW748_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1103911.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            9328..10329
                     /locus_tag="IW748_000991"
     CDS             9328..10329
                     /locus_tag="IW748_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1103912.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            10326..10610
                     /locus_tag="IW748_000992"
     CDS             10326..10610
                     /locus_tag="IW748_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1103913.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            10643..11440
                     /locus_tag="IW748_000993"
     CDS             10643..11440
                     /locus_tag="IW748_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1103914.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            11454..12287
                     /locus_tag="IW748_000994"
     CDS             11454..12287
                     /locus_tag="IW748_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1103915.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            12305..13162
                     /locus_tag="IW748_000995"
     CDS             12305..13162
                     /locus_tag="IW748_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1103916.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            13184..13693
                     /locus_tag="IW748_000996"
     CDS             13184..13693
                     /locus_tag="IW748_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1103917.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            13730..14131
                     /locus_tag="IW748_000997"
     CDS             13730..14131
                     /locus_tag="IW748_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103918.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            14243..14596
                     /locus_tag="IW748_000998"
     CDS             14243..14596
                     /locus_tag="IW748_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103919.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            14630..15073
                     /locus_tag="IW748_000999"
     CDS             14630..15073
                     /locus_tag="IW748_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1103920.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            15090..16175
                     /gene="uxuA"
                     /locus_tag="IW748_001000"
     CDS             15090..16175
                     /gene="uxuA"
                     /locus_tag="IW748_001000"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1103921.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            16168..16881
                     /locus_tag="IW748_001001"
     CDS             16168..16881
                     /locus_tag="IW748_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1103922.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         5965 a         2644 c         3598 g         4876 t
ORIGIN      
        1 gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa aagtaaataa
       61 tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg cagggagcaa
      121 cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc atcaagaaaa
      181 agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta gaaccttgaa
      241 caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa gctattttcg
      301 ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat tgaataaaac
      361 gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg aagttgtagc
      421 tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa caggaacaca
      481 aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag tatctctgaa
      541 aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa gtctaaataa
      601 tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt
      661 atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat
      721 cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc
      781 gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa
      841 agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac
      901 gattggttat attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct
      961 tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact
     1021 attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa
     1081 gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat
     1141 tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat
     1201 gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg
     1261 caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg
     1321 catccattga taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca
     1381 aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt
     1441 ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa
     1501 agttggggct ggattgaatc gtctattgta gaaaattttt aaaaaaactt caagatttgt
     1561 tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca
     1621 agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat
     1681 tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct
     1741 cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac
     1801 tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa
     1861 tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa
     1921 cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat
     1981 cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa
     2041 cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa
     2101 aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat tgtttatgcc
     2161 aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga ccagaaagca
     2221 agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg attttatctt
     2281 cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt ttcaataaag
     2341 aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct attctgtgaa
     2401 caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg ttgtttgtca
     2461 attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag ttctgttcga
     2521 taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac gagcagccag
     2581 aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt atgaaataat
     2641 aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa atgttgtcac
     2701 gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga aagaaggcac
     2761 aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag gggtatgttt
     2821 tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata aaagaatcaa
     2881 aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg ctcaattagt
     2941 gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc acagttattt
     3001 tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt tattaactga
     3061 attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta tagatacgac
     3121 tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag caacgacaac
     3181 ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga aaatttggga
     3241 taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa aaagcagaat
     3301 ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc ctgttctatt
     3361 tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca tataattctc
     3421 ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa gggagcgttg
     3481 gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg agagagcatt
     3541 tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc tggaaatgaa
     3601 acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga gacatctgtt
     3661 actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga agaagcaaca
     3721 gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa agagaatggg
     3781 acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata tttgaattac
     3841 ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa gtcaaaatta
     3901 atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa agagattatc
     3961 aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg
     4021 atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac aggcttctat
     4081 ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa aatgatggat
     4141 gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca tggcggattt
     4201 cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta taaggccggg
     4261 tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga ttatgtgacc
     4321 tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt tcgagagcgg
     4381 ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat gatgtctgta
     4441 ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg atttgcatca
     4501 attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact accggatgcc
     4561 ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt tatgggcctt
     4621 ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc tctacctacg
     4681 attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga tgatgtagta
     4741 cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga tcttttatta
     4801 gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta cacgaaacat
     4861 gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta tgtggatgaa
     4921 aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc ttatggagaa
     4981 aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg
     5041 actgaaggga tttttaatga gacgatacca actattattt tctattacaa aaaaatccca
     5101 gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg aaagaatgaa
     5161 cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt gaaagtacaa
     5221 aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt
     5281 ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga
     5341 aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat
     5401 cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt
     5461 gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga
     5521 tttccagcaa aaaatcgaac aaatcgatat agaaaaagaa atatcctatc cttcaaagta
     5581 caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct
     5641 tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt
     5701 ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc
     5761 ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa
     5821 agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc
     5881 acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca
     5941 tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt
     6001 ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg
     6061 tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc
     6121 cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa
     6181 tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt
     6241 cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt
     6301 cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag
     6361 tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt
     6421 caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat
     6481 ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct
     6541 tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc
     6601 cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt
     6661 ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa
     6721 tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct
     6781 tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt ttgttaggat
     6841 gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata agcaattaat
     6901 gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa taatagaaaa
     6961 tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc aaaaattcga
     7021 tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat aaaattacaa
     7081 atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta attcaccaaa
     7141 atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga aaagataaat
     7201 tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa
     7261 tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg
     7321 taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt
     7381 ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc
     7441 gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata
     7501 gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg
     7561 tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt
     7621 ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg
     7681 tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag
     7741 aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca
     7801 aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga
     7861 cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact
     7921 tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct
     7981 cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga
     8041 tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa
     8101 aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta
     8161 ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt
     8221 tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata
     8281 ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa
     8341 atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg
     8401 gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat
     8461 tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa
     8521 gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat
     8581 ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa
     8641 ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag
     8701 gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa
     8761 tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct
     8821 taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag
     8881 caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga
     8941 tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac
     9001 aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg
     9061 tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa
     9121 gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc
     9181 tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac
     9241 aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac
     9301 atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc
     9361 agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt
     9421 catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga
     9481 ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa
     9541 gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca
     9601 gcaaaagaac accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta
     9661 gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca
     9721 agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac
     9781 aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt
     9841 aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa
     9901 agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt
     9961 ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag
    10021 atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg
    10081 atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa
    10141 gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat
    10201 atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc
    10261 gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata
    10321 aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt
    10381 tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa
    10441 gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt
    10501 tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg
    10561 aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg
    10621 cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg
    10681 agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa
    10741 ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat
    10801 aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga
    10861 tacactagca aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga
    10921 atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca
    10981 ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct
    11041 ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag
    11101 aaatgtggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg
    11161 ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg
    11221 ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct
    11281 agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat
    11341 tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca
    11401 gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa
    11461 cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct
    11521 ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg
    11581 tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga
    11641 tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag
    11701 ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg
    11761 cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt
    11821 ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat
    11881 caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg
    11941 ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg
    12001 ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg
    12061 tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg
    12121 ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg attattatcg
    12181 ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca
    12241 attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg
    12301 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
    12361 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
    12421 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
    12481 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
    12541 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
    12601 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
    12661 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
    12721 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
    12781 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
    12841 cactatgggc cgtaatgcta caggcctaaa gcaaaacact ggtgttcaga cagcgattga
    12901 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
    12961 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
    13021 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
    13081 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
    13141 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
    13201 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
    13261 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
    13321 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
    13381 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
    13441 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
    13501 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
    13561 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
    13621 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
    13681 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
    13741 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
    13801 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
    13861 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
    13921 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
    13981 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
    14041 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
    14101 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
    14161 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
    14221 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
    14281 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
    14341 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
    14401 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
    14461 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
    14521 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
    14581 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
    14641 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
    14701 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
    14761 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
    14821 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
    14881 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
    14941 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
    15001 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
    15061 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
    15121 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
    15181 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
    15241 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
    15301 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
    15361 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
    15421 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
    15481 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
    15541 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
    15601 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
    15661 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
    15721 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
    15781 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
    15841 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
    15901 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
    15961 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
    16021 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
    16081 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
    16141 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
    16201 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
    16261 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
    16321 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
    16381 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
    16441 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
    16501 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
    16561 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
    16621 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
    16681 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
    16741 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
    16801 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
    16861 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
    16921 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
    16981 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
    17041 cgtttaaaga gaagaacgag aatcgtccat atgagggttt cca
//
LOCUS       DACENJ010000041        16619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.041, whole genome shotgun sequence.
ACCESSION   DACENJ010000041 DACENJ010000000
VERSION     DACENJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.041"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(86..1051)
                     /locus_tag="IW748_001002"
     CDS             complement(86..1051)
                     /locus_tag="IW748_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1103923.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1285..2130
                     /locus_tag="IW748_001003"
     CDS             1285..2130
                     /locus_tag="IW748_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1103924.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2321..3508
                     /locus_tag="IW748_001004"
     CDS             2321..3508
                     /locus_tag="IW748_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1103925.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3514..3870
                     /locus_tag="IW748_001005"
     CDS             3514..3870
                     /locus_tag="IW748_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1103926.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3872..4210
                     /locus_tag="IW748_001006"
     CDS             3872..4210
                     /locus_tag="IW748_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1103927.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            4605..5639
                     /locus_tag="IW748_001007"
     CDS             4605..5639
                     /locus_tag="IW748_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1103928.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5632..6318
                     /locus_tag="IW748_001008"
     CDS             5632..6318
                     /locus_tag="IW748_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103929.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6342..7190
                     /locus_tag="IW748_001009"
     CDS             6342..7190
                     /locus_tag="IW748_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1103930.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7387..8160
                     /gene="sufC"
                     /locus_tag="IW748_001010"
     CDS             7387..8160
                     /gene="sufC"
                     /locus_tag="IW748_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1103931.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8173..9459
                     /gene="sufD"
                     /locus_tag="IW748_001011"
     CDS             8173..9459
                     /gene="sufD"
                     /locus_tag="IW748_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1103932.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9456..10691
                     /locus_tag="IW748_001012"
     CDS             9456..10691
                     /locus_tag="IW748_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1103933.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10678..11148
                     /locus_tag="IW748_001013"
     CDS             10678..11148
                     /locus_tag="IW748_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1103934.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11153..12544
                     /gene="sufB"
                     /locus_tag="IW748_001014"
     CDS             11153..12544
                     /gene="sufB"
                     /locus_tag="IW748_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1103935.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12639..13781)
                     /locus_tag="IW748_001015"
     CDS             complement(12639..13781)
                     /locus_tag="IW748_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1103936.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13820..14554)
                     /locus_tag="IW748_001016"
     CDS             complement(13820..14554)
                     /locus_tag="IW748_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1103937.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            14786..15061
                     /locus_tag="IW748_001017"
     CDS             14786..15061
                     /locus_tag="IW748_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1103938.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            15159..>16619
                     /locus_tag="IW748_001018"
     CDS             15159..>16619
                     /locus_tag="IW748_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103939.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLN"
BASE COUNT         5467 a         2945 c         3483 g         4724 t
ORIGIN      
        1 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
       61 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      121 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      181 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      241 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      301 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      361 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      421 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      481 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      541 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      601 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      661 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      721 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      781 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      841 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      901 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
      961 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1021 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1081 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1141 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1201 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1261 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1321 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1381 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1441 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1501 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1561 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1621 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1681 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1741 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1801 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1861 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1921 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     1981 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2041 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2101 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2161 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2221 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2281 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2341 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2401 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2461 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2521 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2581 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2641 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2701 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2761 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2821 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2881 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     2941 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3001 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3061 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3121 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3181 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3241 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3301 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3361 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3421 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3481 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3541 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3601 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3661 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3721 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3781 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3841 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3901 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     3961 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4021 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4081 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4141 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4201 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4261 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4321 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4381 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4441 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4501 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4561 aggctttttt gtttgaaaaa tcataagaga aagatgtgaa tggcatgtca ttaatccaac
     4621 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4681 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4741 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4801 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4861 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4921 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     4981 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5041 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5101 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5161 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5221 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5281 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5341 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5401 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5461 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5521 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5581 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5641 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5701 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5761 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5821 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5881 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     5941 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6001 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6061 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6121 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6181 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6241 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6301 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6361 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6421 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6481 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6541 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6601 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6661 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6721 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6781 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6841 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6901 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     6961 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7021 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7081 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7141 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7201 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7261 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7321 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7381 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7441 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7501 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7561 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7621 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7681 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7741 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7801 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7861 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7921 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     7981 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8041 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8101 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8161 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8221 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8281 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8341 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8401 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8461 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8521 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8581 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8641 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8701 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8761 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8821 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8881 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     8941 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9001 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9061 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9121 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9181 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9241 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9301 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9361 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9421 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9481 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9541 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9601 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9661 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9721 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9781 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9841 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9901 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
     9961 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10021 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10081 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10141 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10201 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10261 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10321 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10381 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10441 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10501 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10561 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10621 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10681 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10741 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10801 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10861 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10921 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    10981 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11041 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11101 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11161 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11221 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11281 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11341 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11401 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11461 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11521 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11581 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11641 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11701 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11761 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11821 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11881 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    11941 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12001 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12061 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12121 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12181 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12241 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12301 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12361 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12421 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12481 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12541 gtaaacctgt tataggagct agaacccttg atatataagg gttctagctt ttttgttttg
    12601 cctgtatact agaaatgacc agattttgac cagctctttt actgatgagc catcatatca
    12661 gcaaagtctt gacctgtttt cttaggtgca ttatttgtaa tatgtgcata gatgtctaat
    12721 gtcactttac tattttcatg ccctaatcgt tgttgaacta ccttgacaga ttctcctgct
    12781 tctaataata aactagcatg tgtatgtcta aacccatgag gtgtaatacg aggcaagtta
    12841 tattttttca aaatatatct taaccaatca ttaacagtgt ttggaatgta tagagtgttt
    12901 ttaactgttg taaaaacatg ttgctcttta gagctagtat tgtatccaag ttttaaatac
    12961 tcttcttttt gctgtaattt ccagttgttg aggatagaaa gagtctcagt atctaaagag
    13021 ataactcgtc tagaagctct tgttttcgga gattgaatta taactttacc aaattcatct
    13081 acagcaagag ttttattgac atgtagttct ttattaaacg tgtctatgtc tttccactgt
    13141 aaagcgagta cttcactctt tctcatacct gtgtatgcca ataatctgaa gaaggcgtat
    13201 tgtttcatgt taccaaaatc tttaaaagca tccagtaaat tattaagctc ctcttttgag
    13261 taaaatttac gatttttctc atactctttt tttcgtggaa gaagtgtttt ttccatagga
    13321 ttattgtcaa tatattgcat tgcaaccccg tagcgtaaaa tttgtgcagt ctgttttctc
    13381 atataatcgt aactttcata tttactgtgc cattcatcta ccactttttg acaatatggg
    13441 acagagatat tcgatagttt aagcttacca aatgctggaa gtatttgatt atttgcgtat
    13501 cttactgaga ttgcgattgt tgaagcttta acactggtac gctgatgagt tatccattca
    13561 ttatacaggt cttcaaatgt catatcaggt gctttctttt taacaccgta taatatttga
    13621 tgtctgtact cgtcataatc tttttgagct tcttgtttgc tactaaaacc tcttctcgta
    13681 gtatttattt tttcaccagt tcgtggatcg gtggcaatgt gtcctttata catccacgcc
    13741 acttgaccgt ttttcttttt gtactttgat atttttgcca ttaaatcatc tcctcttagt
    13801 ttacaaggag tacttaccac tagatggtat cacctccctt catttttttc ttagtgtatt
    13861 ttttcccaat tgatgaaatc gtttgttcta gcttgtttat attatcagta atttctctca
    13921 cagcttcttc gggataagac atgtctttca attgaatagg tagccattca tctaccattc
    13981 tttctagtag ttcttctata ctgctagtga ggaaaagaat gttattgtca aattcttcta
    14041 caatagcatt gatgaataat tcttctattt ttaagatatt gaaaagacta attttttcat
    14101 ttttaacttt attacaagtc ttgttagcaa taaattcttg catctctggt tctaatttgg
    14161 aatatctatc cagccatgta tctaaattag agtgtactgg tgggaactgt tgacgaagat
    14221 gctggaacag gtttttatct ggaaatgtta taccatctag tacaatgcta tctctataaa
    14281 gaataattcc caaaatatat tcttctacat tgccacaaac taactcctct atactcatat
    14341 ttcccaaatc agcaattcgg gataatcgct ctggtattgg taaataggtt cccttctccc
    14401 attcgtagat agtttttttg tcaattaatt gaccgttgtt aatcataaga ccaaatgctg
    14461 gttgtgttaa ttttttactc tttcttatag cttttatttt tgttccgagt atcttttttc
    14521 gttctaaata atttattggt ggacgtttta tcatattgac cacacctcct aaaattagta
    14581 tacctcaaaa aaggggtgtt gtaaataaat tttacaaagc tgttgacacc ccttttttat
    14641 cgaggtatta tgtgtatgtc gatgaactgg taatcatttt ttgtttgatt aatcggttaa
    14701 taacaaaata cttttatttt gagcaaaaaa tacgaagata attcttagaa aatttgttca
    14761 agcaatcacg aaaggagggg gaaatatgtc tctacaaatc attgatagca ttgtttctat
    14821 acaagttgaa caacaagtga aagagataat tacagtgcta aagcctatgc tagaagacat
    14881 tatttctgga gtcactctag agtatatgga ctatcagcag gcaagtgaat atctaggtgt
    14941 atcagtagga acgattagaa aatatgtttc tcaatacggg ttacctgtta taaaaattga
    15001 tacggtcatt agatttaaaa aatctgatat tgatagtttc atggaacggt ataaaggttg
    15061 agtaaacaaa taagactaaa acatatagta ataaagagtt tgtttttatt atctgtatgt
    15121 tttagtcttt aacagaattt tataaggagg aaacacaaat ggaaacatca atgattaata
    15181 ttccaattta tgtagatcaa gaaaaattat tagagaagcc agttacaaaa aaagaaaaaa
    15241 ctgatgtaag agataggtgt ttagataact atagagaagt tacaagagat gagtttgtag
    15301 agatactaaa ttcatcacaa tcatttattc caagcaagac aaagaataaa ggcaatgaca
    15361 aggagagttt tgtggaaacc agagtaatta ttctggatgt cgataacact gttaaagatg
    15421 agaaggggaa ggtagtagat ttgagtaaag atgatagtag atacttaagc atagaaaagg
    15481 ttttatctat caatttagtt cataattctg cgtttgcgat acaaaaaagt attcggtact
    15541 ctgaaaatct agaaaaatac aagattgtct ttctattaaa agaagcaatt actgactatg
    15601 gtaaaatggt tgctgtttac gaatacttga aaaagaacat ccccgggtgt gatgacaatg
    15661 taaatgcttc gaatagaatg ttttttggag gatataagtc agatgcagtg ataataatta
    15721 atgaagacaa catgttagat attactgagc ttccgattga ttttaccatg acatgttcta
    15781 aaaattgtca aagtgattca gggagagagt atgaactagt caatgaaacc gattttgtta
    15841 agttgataaa aaatgacgac aaagaagaaa tgaaacagtg gtttaaagac tgcctgtttg
    15901 attcctccga catgtctttt aatgagattt atgaaagatt gttaaccatt gatatgaata
    15961 aattacttaa gtcaagtaaa aacttaagat gtctgtttca caacgatcat aatccctctg
    16021 ctagtatttt taccagtaaa actgggcatt ccatttttta ttgtcattcc agtaattgtg
    16081 atatcagtga aaattttatt ggagttgtga tgctaatttt aaagaaagac agtcgggtag
    16141 aaacatttaa ttggctatta gagaacctag acttgtatcc tacacatttt cgaaaattga
    16201 aagaggaatc aaaagtactt tttgacactc ttgaatcaat gaaagacaaa cttttcagga
    16261 caataaggaa ttatctagag gaaatgcgac gtgtctatga tgtcttgctt tctgatgtta
    16321 atttttttga tgatagtaag gaaagtgtga cttgtatttt gtcgggggaa caattagcta
    16381 aaaaaatgag ttcttactat ggtaaaactt atgacataga taaatgtaat aagttactat
    16441 cacttatgac ttttatagga ttgataaaaa aattagatga tgaagatgtt cctttgaaga
    16501 tgctcgaata cctaaatagg ttaaaggaag agaaagcgtt aggaaacttt aaacataaga
    16561 gaagcaatgt ctatcgatta gacgttttta atgcaacttc tgtgattcaa aaattgaat
//
LOCUS       DACENJ010000042        16576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.042, whole genome shotgun sequence.
ACCESSION   DACENJ010000042 DACENJ010000000
VERSION     DACENJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.042"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            26..1132
                     /locus_tag="IW748_001019"
     CDS             26..1132
                     /locus_tag="IW748_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1103940.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            1149..2177
                     /locus_tag="IW748_001020"
     CDS             1149..2177
                     /locus_tag="IW748_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ1103941.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            2183..2662
                     /locus_tag="IW748_001021"
     CDS             2183..2662
                     /locus_tag="IW748_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1103942.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2678..3973
                     /locus_tag="IW748_001022"
     CDS             2678..3973
                     /locus_tag="IW748_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ1103943.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            3984..4808
                     /locus_tag="IW748_001023"
     CDS             3984..4808
                     /locus_tag="IW748_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1103944.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            4973..5872
                     /locus_tag="IW748_001024"
     CDS             4973..5872
                     /locus_tag="IW748_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1103945.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(5998..6867)
                     /locus_tag="IW748_001025"
     CDS             complement(5998..6867)
                     /locus_tag="IW748_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1103946.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            7020..8480
                     /gene="rhaB"
                     /locus_tag="IW748_001026"
     CDS             7020..8480
                     /gene="rhaB"
                     /locus_tag="IW748_001026"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ1103947.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            8477..9733
                     /gene="rhaA"
                     /locus_tag="IW748_001027"
     CDS             8477..9733
                     /gene="rhaA"
                     /locus_tag="IW748_001027"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ1103948.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            9747..10592
                     /gene="rhaD"
                     /locus_tag="IW748_001028"
     CDS             9747..10592
                     /gene="rhaD"
                     /locus_tag="IW748_001028"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ1103949.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            10594..10905
                     /gene="rhaM"
                     /locus_tag="IW748_001029"
     CDS             10594..10905
                     /gene="rhaM"
                     /locus_tag="IW748_001029"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ1103950.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            10987..12138
                     /gene="fucO"
                     /locus_tag="IW748_001030"
     CDS             10987..12138
                     /gene="fucO"
                     /locus_tag="IW748_001030"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ1103951.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            12265..13023
                     /locus_tag="IW748_001031"
     CDS             12265..13023
                     /locus_tag="IW748_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ1103952.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            13123..13767
                     /locus_tag="IW748_001032"
     CDS             13123..13767
                     /locus_tag="IW748_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1103953.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            13774..14781
                     /locus_tag="IW748_001033"
     CDS             13774..14781
                     /locus_tag="IW748_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ1103954.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            14797..15639
                     /gene="kduI"
                     /locus_tag="IW748_001034"
     CDS             14797..15639
                     /gene="kduI"
                     /locus_tag="IW748_001034"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ1103955.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            15659..16462
                     /locus_tag="IW748_001035"
     CDS             15659..16462
                     /locus_tag="IW748_001035"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1103956.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT         5345 a         2852 c         3780 g         4599 t
ORIGIN      
        1 aagcgtttaa aaaagtggcg taaagatgat tattaaagaa gagatttttg tacctaaaga
       61 attagtgaaa gaaaaaattg acctgctcgt tgaaaacctg acaacgatca aagacgataa
      121 tggtgaattt ttgttggact ttgatggact gaaagtagat gacaaaagct ggacagtgtg
      181 gaattggcca caaggtgtcg gtctatatgg tatttataaa aattaccaga tgacaaaaaa
      241 tcctcgcgct tatcaagtgg tcaatgaatg gtttgaggat cgtatggaag aaggcgctcc
      301 gcccaaaaac gtcaatacaa tggcaccgct attgactatg gcttacttgt atgaagacac
      361 gaaagattca cgctatttgc cttatttaga acaatgggca gaatgggtca tgaatgacat
      421 gcctcggaca aatgaagacg ggttacagca tgccacatat ggaccggaaa ataagaatca
      481 gttgtgggac gatacattga tgatgacagt gcttcctttg gcaaaaatcg ggaaactgtt
      541 gaatcgccca gaatatttag aagaagctcg ttaccagttt atgatccata tcaaatactt
      601 gatggataaa cgaacaggat tgtggtatca cggctggacg tttgaaggaa atcataacta
      661 tgccgaagca ttttgggctc gtggaaactg ctggttgacg attgctattc cagagatcat
      721 tgaaatctta gaattatcga aagaagatgc attgcgcaaa ttattgatcg aaacacttga
      781 agcacaagtg cgtgcattaa aaacttatca gtctgaaagc ggtttatggc atacgttgtt
      841 ggatgatcct tcttcttacg tagaatcttc tgcaactgca ggatttgctt acggtatttt
      901 gaaagcggtc cataaacgtt atttaccaca agaatataaa gaagtcgctt atcgtgctat
      961 ccaaggattg cttgaacaaa tcgatgaaaa aggagaagtt caaaacgttt caatcgggac
     1021 aggaatgggg gacagtttag atttttatcg gaatatcgga atcacagcaa tgccttatgg
     1081 acagtcgtta acggtgcttt gtttgacaga attgctcgtc tcttattgtt aaaagaaagg
     1141 aaacccatat gaaaaaaaca tcactgctgt ttgcagcaag ttcgcttagc ctaggtctgc
     1201 taggtctaag cggctgcgga aataattcta gtgtggcaac tgtcgcagat gcagatgtaa
     1261 atgagatagt taccttgcgc tttgcttatg caagcaacag ccagccagta attgatgcaa
     1321 tgaatgaatt tgggcgttta gtaaaagaaa aaacagatgg ttccgtacag atccagtatt
     1381 tcccagatgg acaactgggt ggagaacgtg aactgatcga attgacacag tcaggagcag
     1441 tagatttcac aaaagtaagt gcctcagcgt tggaaagttt ctctaaagat tattcgattt
     1501 tttcagttcc ttatctattc aatgatgaag aacatttctt taaggtgatg gataatgaag
     1561 aaataatgga gccaatctac caatcgacca aaaatttagg atttaccgga ttgacctatt
     1621 atgattctgg tcagcgcagc ttttatatgg tagacggtcc gatccatacg ccggaagatt
     1681 tgaagggaaa gaagatccgt gtgatgcaaa gcgaaaccgc tatcaaaatg gtagagcttt
     1741 taggcggttc gcccgtacct atgggaagtg atgaagtgta cacgtctctt caatccaatt
     1801 tgatcaacgg atcagaaaac aatgaatttg tcttgtatac agctggacat ggtggtgtgg
     1861 ctaaatacta ttcctatgat gagcatacac gtgtgccaga tatcatcatc atgaatgatg
     1921 ccatcaaaga acagctaact gatgagcagg aaaaagccat tgaagaagca gcgaaagaat
     1981 caacggtttt tgaaattgaa gcttttgcaa aagcaattga agaagaaaaa gctttagcac
     2041 aagaagaaca tggagtccaa ttcaatgatg tagataatac accgtttcga gaggcagttg
     2101 ccccacttca tgatatattc aaaaacgatc ctacctacaa agacctttat caaaagatcc
     2161 aagagcaagg agcgtagctg tgatgaaaat gattttaaac aaggtattag agatcctggg
     2221 agctgttttg cttgttggga tggtcgctgt tgttttatgg caagtatttt ccagaacggt
     2281 tttaggaaat ccaaatacgg tcacggaaga gcttgttcga ttcggtcttg tctggtttgc
     2341 catgttgtct tccgcgtatg ttgtgggaca aaaagcacat ttagccgtaa cggttttaag
     2401 tgaaaaatta actggcaaaa aaagtaaaat tttagaagtt gttgttcaaa gtttatttct
     2461 ggtatttgct gcgatgatca tggtgtatgg cggatggaat gctgtcacat tgacaatggg
     2521 gcagatctcg ccttctttaa gtattcctat gggatatgtt tatctgtcag tacctgtatc
     2581 gggggtactc atcattatct atagcctgat caatctaatc gatacattaa gcaacaagcc
     2641 agcaattcaa gaaaaaatat aaaaatggag gaataaaatg gcaatagaag ccggattagt
     2701 gttatttttt gtatttgttg tgttgcttgt attagggatg ccgattgcta taagtattgc
     2761 attctcatcg atgatgactt tattgttagt tataccgttt gatgtttctg cttttagttc
     2821 agctcaaaaa atggtgggaa gtatcaatag tttttcgctg atcgctattc cgtttttcgt
     2881 tctctcgggc attatcatga ataatggagg gatagctaag aaactagtgg attttgcgat
     2941 gttgttcgtc gggcgcgtgc cgggggcttt ggctcatacg aatgtgttag ggaatgcttt
     3001 atttggttcc atctctagct cggccattgc cgcttctacc gcgattggcg gagtgttgat
     3061 ccctcagcaa gtggaagaag gatatgaccg gaagtttgcg acagcggtca atatcgcgtc
     3121 agctccgaca ggtatggtca ttcctccaag tactgctttt atcatgtatt cacttgttgc
     3181 tggtggtgct tcgatctctg ctttgtttat gggaggttat ctcgtaggcg ctttgtgggc
     3241 gctaggaatc atgctagtag cattcatcgt tgctaaaaag aataaatacc ctgttgtcga
     3301 aaaaggacag atggctcatg ctggaaaaat aataaaagag gcgattccta gccttctatt
     3361 gattgtcatc attatcggtg gtatcttgac ggggatattt acagcagtag aagcttcagc
     3421 gatagctgtc atttactccc tgttgattgc tatgttttac tataaaacgg taaaactaaa
     3481 agatatacct aatatgttgt ttcaatcagt agcaatgtca gggacgatca tgttcttatt
     3541 ggctacttct tccatgatgt ctttcgccat ggcgtttacc ggtattcctc aggcaattag
     3601 ttcgttgatt atgggcgtaa cggataataa atttattatt ctgttattag tcaatcttgt
     3661 gttattactg gtgggtatgt ttatggatgt ggcgcctgct atcttgattt ttacaccaat
     3721 atttttacca atcgtcacaa gtgtaggggt tgatcctgta cattacgggt tgttttcaat
     3781 catgaatctg tgtgtaggct ccatcacgcc gccagtaggt acaggactat atgtcggtgc
     3841 aagtgtagga ggcgtaaaag cagaggcaat gctgaaaccg cttcttcctt tttatggggt
     3901 tatcttagtc gtcttgtttt tgattactta cttcccgcaa atcgtcatgt ggctacccaa
     3961 tatgctgagt taggagaata gttatgttga acattacaat tagaggacat gatctttccg
     4021 aagtccaaac gatagaagat cttgcagaaa aaacaaaaga acaagggatt catacactgc
     4081 agctcgcctt aggtctttcg tttcctgaaa taccttctgg cgcaaatgag atcaattctg
     4141 ggatgggaaa ttatgtcaaa cgagttttag aaaagcaaga agtgaccgta ggaattttaa
     4201 gttgctatat caatatgatt catccagatt taacgatacg acaagagttg ctgacaaagt
     4261 tcgagcagta tcttcgctat gcttcttcct ttggtgcgtc gatggttgct tcggaaactg
     4321 gatgtgtttt gccggaaatc caatatacgg aagaaaattt cacagatgaa gcatttgctg
     4381 aagctgtgtc cgttatccgt cgcttggtaa aagctggaga aaaataccaa atgatggtgg
     4441 ggatagaacc aggtttgaat catccggtgt attctttagc aagggtagag cagctgatcc
     4501 aagcagtaga ttcggactat ctaggaatca ttttagatcc gaccaatttg atcacaagta
     4561 gtgattatca agaacaagtc caattggtag aagaagcttt tgaacgtttt ggtgaaaaga
     4621 tatgtgcggt tcatttgaaa gattttcgtg tcgaacagga aaaaatcgtg ccggtaaatt
     4681 tgggagacgg tgtgatcgag tacacaaaaa tcaaagaaat catcatgaag aatcgtccgt
     4741 acctgtatgt ggttcttgaa gaaacaaaag atgatgggat tcgttatgga agaagtcttc
     4801 ttgaataatg taaagaagac gttggttaac taatatttag aatggagggg gcggggctag
     4861 aagatgccac gtctcttctt ttttataaga gtatataaaa gcagctcttt ttgattacaa
     4921 aaaacgtggt ataataaaat tgtgcataag tgaacaaaag gaggtgcggc acatgctcca
     4981 aaagaaaatc agcggtgaac aagtcaatac gatggtttct gatcagtatg aaatctatca
     5041 tgtcaaagat gtcgtcagcc aaaataaaac gatctatcat catcatgatt tctatgagat
     5101 ccatgctaca ttaaagggag aagcgacttt ttatttgaac ggacgacaat ttacgatcaa
     5161 agcaggaagt gtgctgctca tccattccaa tgatttgcat cgtatcgtcc gccaatcttc
     5221 agatgtattc gagcgtgtct atatctttgt caccccttct tttctagaaa gtcgttccac
     5281 gccatggtct aatctatcgg cttgctttca accaatggga gagaagagga gtcgtgtatt
     5341 gcaaatagaa caggaagtat tgaagcaaaa attatcattt atcgaccatc ctcctgataa
     5401 aaaaatttac ggtgaagaca taaaatatga acaagctctg gtagaatact tgatttttct
     5461 caatcaagtt gtacaaaaag aagagagtgc gcaagaacag aatgctgtag ttcctaatga
     5521 gcgtgtggaa caaatgttac aatatatctc aaaaaattta tctcatccgc tttctttaga
     5581 ggaaatggaa aagcagtttt ttgtcagcaa ataccatatc acacgagaat ttaaaaagta
     5641 cactggattt acgtttcatc agtatgtaat gaagaaaaag ctgatctatg ccaagcactt
     5701 gttgcgagaa taccgcagct ctagtgctgt atatagtaaa agtggttttt cttcttatcc
     5761 gcattttctg aaagcattca agagagagtt cggcataacg cctaaagaat ttttgaaacg
     5821 tgatacacag aaacagttgg ttcattatga acattttgaa gatgaacaat gagaatgatc
     5881 actaaaaaat aaccgcagta gctgctgttg tttacataga agcagaaact gcggttattt
     5941 tatttttatc tatatatttc aaaatatttt gaaaaaatga cgtacatatt atatgaatca
     6001 gctgaattta cgaaagtctc tcggcgatag tcctgttttt gctttgaaca ggcggctaaa
     6061 gtaagcaaaa ttctcaatcc ctatttcttc ggaaatcatt cgaaccgtaa attctgtata
     6121 ccgaagaagg tctttagcgt aagaaagttt tttgctgatc aaataatcga ttggtggaga
     6181 accgataaat ttggaaaaat ctttgctcag ctgatattta ttcaataaga attgttgttc
     6241 taattgatca agtgtaatcg tttctttgaa atgttcgtct aagtattgct tcagtttgta
     6301 aacgtaggcg ggaatatcct cttcttcgaa atctagctga tacttttgca aaatcagttc
     6361 attcagcaac tcgtggatag ctacagagga ctggaaatcg gtacgggcat tcgtgccttg
     6421 ttgcatgtgg atcaattctg tgatcaaatg gtggatcttg ttttctgatg gatcccctgt
     6481 tgaatggaag gtgttatggc cgtcttttgt gaactcctga taaaatgatt ggacgttctg
     6541 accattcaaa tggatccaat ccatttccca tggttcttcg ctgatcgttt gataacgctg
     6601 atattcttgg cagtcaataa aaaataagtc tccagctttt agctgttgct gattatcatt
     6661 atatgtaagc gaacctttgc cagacaaagt aaactttatc aagtaagaag gtaaatgtgc
     6721 tcgttcagta tagtaaggga ttggtgcttt gaagtaaccg atttcttgga tataaaaaaa
     6781 taaattcctg cttctttgtg taggcgtcaa gatcactctt tttgagtctt ccgaccatga
     6841 gtggttggct ttttcccagt aatccatgta aacactccca acttgtcaag ataatgcaat
     6901 tatataccaa aatagtgtga agcaaaatat aagattctgt attatgatag cattgttaat
     6961 aaatgataac gctttattaa taaagtgcta atcaacaata aagtaaaaga ggtggagaaa
     7021 tgaatccaac atttgttgcc atcgatatcg gtgcatcaag cggacgacta atgaaaagta
     7081 cgagggatgc aaaaggttct ttgatcttgg aagaagtaca ccggttcaaa aacggctttc
     7141 ggaacaaaga cggtcacgat cgctggaaga tcgattatct tatcaaagag attttgattg
     7201 gatttgaaaa attaaagcag acagggattg atcgcctttt tgcggggatc gatacatggg
     7261 cagtcgatta ctgtctgatt gatgaacaag gggatcggat aaatgatccg attgcttatc
     7321 gggacaagcg taccaatgga gcagtagaac agttttctga aatctattct ctagataagt
     7381 tgtatgagca gacagggatt cagatccagc ctttcaatac cttgttccaa ttgtttgctg
     7441 aagaaaaaga aaatttggca aaagcacata aattgttact gatacctgat tatttagggt
     7501 atgtctttac tggtaacatg gtgacggaga aaactaatgc atctactatg cagctgctga
     7561 atgcagggac aaaagaatgg gaaatagaac ttcttgaaaa aatcggtgtc tcgaaggaat
     7621 tatttccacc attgacagag ccgggaacga tactaggaga tctgcaaaaa gaacaatttt
     7681 caaaatatga tttgccagat gtgaccttta tcacagtagc aagtcatgat acggcgtcag
     7741 ctgtattagg aacgccaggt caaggagaac gttggggtta tatcagcagt gggacatggt
     7801 cgcttttagg gattgaaaca aagatcgcca atgtttctcg caatgctttt tctgaaaatt
     7861 atacgaatga atggggtgcc cataacacgg tgcgtttttt gaaaaacatc atggggatgt
     7921 ggttgatcca ggaagttgcc cgaatgcaag agtacgagta cagttatgcg gaattggcag
     7981 agcttgctgc aaaagaaaaa ccgctgcagc aagtgatgga aatcaatgac cctcgatttt
     8041 tgaatccccc aaatatgata gaagagattc agcaatactg ttttgaaaca gagcagccag
     8101 tccctgtctc atcgggagct atcgctcgtt gtgtgtatga taatcttgct ctttgttacg
     8161 cacaagagct gatcaaagta gaacacttaa ctggaacgaa attggaaaag ctgcacatcg
     8221 tagggggcgg atcgaataat cgtctactga atcaattgac agcagattat gccggaatca
     8281 ttgtagaagc aggacctagt gaagccacag caatcgggaa tgttgccatg caaatgctca
     8341 gtacaggtca gttttcgaat atagaacaag caagaaatgt aatcaaacag tcgtttccat
     8401 gtgaaacttt ttacccagat gaaaataagc aaaacacgat ggaatggtac gtgaataaaa
     8461 tcaaaggagt aatcgtatga acacgattga aagaaattac gaacaagcaa aagaaaaata
     8521 tgcaactatc ggtgtagata cagatgctgt actagaaaag atacaggaca tcaaaatttc
     8581 tatgcattgc tggcaaggag atgatgtcaa aggtttcttg acaccggatg gcgaattgac
     8641 cggtggaatc atggcgacag ggaattttcc aggagccgca cgaacacctg aagagcttag
     8701 acaagatctg gagaaggctt attcgttgat accgggcaaa cacaaattga accttcatgc
     8761 tatctatttg gatacagaag aatctgtcga tctaaatgaa attgagccga aacattttga
     8821 aaaatgggta gagtgggcaa aaaaagaaga aatcggtctt gacttcaatc cgactttctt
     8881 ttcgcatcca atgatgaaag acggctttac attggctcat ccagataaag aagttcgtga
     8941 atactggatc gaacacggga aaagaagccg caaaatcgcg gagtactttg ggaaagaaac
     9001 tgggcaaaca agtatcaata acttttgggt acctgatggg atgaaagata atccgattga
     9061 tcgttttacc ccacgggaac gtttaatgga atcactcgat gaaattttta cagaagagtt
     9121 agatgaacgg tatgtacaag atgcagtaga aagcaaacta tttggtttag gcgctgaagc
     9181 ttatacagtc ggttcccatg aattttatat gggttacggg ttgactcgtg acaaacttgt
     9241 ttgtctagat gctggacatt ttcatccaac agaagtgatt tccaataaat tatcttcatt
     9301 agctttattc agcaaaggaa tcatgttgca tgtttcccgt ccagtgcgtt gggattcaga
     9361 ccatgtggtt ttgatggatg atgaattgca ggaaatcgct aaagaattag tccgtaacga
     9421 attgttggaa aaaacaaata tcggtctgga tttctttgat gcaacgatca accgtatcgc
     9481 tgcttgggtc atcggtacga gaaatacaca aaaagcactg ctgaaagcta tgctggaacc
     9541 agttgagcga ttgaaagaaa tggagctggc ttttgacttt acttcaagaa tggcttatac
     9601 ggaagaattg aaagatttcc cgtatgccga tgtatggaat tatttctgtt acaaaaatca
     9661 agttccagtt ggtttggatt ggctggaaga agtccaggaa tatcaaaaag acgtgttgga
     9721 attaagaaaa taggagtgtg gctatattga aaaaaataga tattttacaa gcgccttatg
     9781 tgaaagaaat gatcgaaacg acgtcgaatc tttatcgctt gggatgggat gaaagaaatg
     9841 gcgggaatat ttcttattta ctaaaagaag aagagatcac accgttcttg gatcctgaaa
     9901 aagtgatccg ccgcatctcg atgatttttg atgccagccg tttagcagga tgctatttta
     9961 ttgtaactgg ttcaggaaga tattttaaaa atgtagcgag cgaacctgcc gagaatcttg
    10021 gaatcgttcg agttgctgaa gatggcaaaa cactggaact tttgtggggg ctggaaaacg
    10081 aatgccttcc tacaagcgag ttgccaagtc atttcatgag tcatatcgcc cgtttagaag
    10141 tagacccaga taatcggatc gttatgcatt gccatgctag tcatctatta gcgatgagtt
    10201 ttacgcatga attagacgaa cgttccttta gtcgtacgct atggcaaatg tgtacggaat
    10261 gcttagttgt ttttccagaa ggaatttcca tcattccgtg gatggttccg ggaacaaatg
    10321 agattgggga agcaacagct gaaaaaatga aagaaactcg attggtttta tggccacaac
    10381 atggaatcta cgggtcagga aaagacatgg atgaagtctt tggtttgatc gagacagcag
    10441 aaaaggcagc agaagtctat acgtacgtca aagcgcaagg accaatcttg caaacaatca
    10501 cggacgaaaa cctgtggcat ttagcagatg cgtttggtgt cactccaaaa gcaggttatt
    10561 tggaggaagt tcatacgaaa gcaggtgtct gacatggtga aaaaagcttt ttgcatgaaa
    10621 gtttacccag aaaaacaaga agaatacaaa aaaagacatg atgaactttg gccagaaatg
    10681 cgtacagcac ttcgtgaaca cggggtcatc gactattcga tctatcttga tccgagtaca
    10741 agtttgttat ttggttactt agtgatcgaa gatgaaaagt tatgggaaaa aatggcaaat
    10801 acatctatca atcaaaaatg gtggaagtac atggaagata tcatggagac aaatccagat
    10861 gcctctccag tcagttggga attgatgcca gtctttgaac tgtagcaaca agcaactggg
    10921 caaccggttg cttggggttc tttcgttttt attgtgaaaa ataatacaaa ttaggaggaa
    10981 tagaaaatga gtaatcgaat gattttaaat gaaacatcgt attttggaaa aggagcgatt
    11041 gagtcgatcg tccctgaatt tcagcagcga ggattcggca aagcagctgt cattactgac
    11101 aaaggattga tcgagcacgg tatagcaaca aaagtaacag aacttctaga taaagcagca
    11161 atcgcttatg ctttatacga cgggatcgta cctaatccaa cgatccaaaa tgtaaaagat
    11221 ggagtggctt ttgttaaaga ggcagaggca gactgtttga tcgcaatcgg tggaggttcg
    11281 ccaatcgata cggcaaaagc cattgggatt attttgacga atccagagtt ttcagacgtc
    11341 attagtttag aaggtgttgc tgatacgaaa aatccatgtt tacctatatt agctatacca
    11401 acgacttctg gaacagcagc agaagtaacg atcaactacg tcatcacaga tgaagtaaat
    11461 caccgaaaat ttgtttgtgt tgatcctcat gatatcccaa tcgtcgcgtt catcgacagc
    11521 gatatgatga tgggaatgcc gaaaaaattg gctgcatcaa caggtatgga tgctatgacg
    11581 catgctattg aagggtttat cacaaaaggc gcttgggaaa tgactgatat gctgcattta
    11641 aaagcaatcg aaatcatcgg ccattctttg gaagcttctg tagatggcga tcaaaatggg
    11701 cgagaaaaaa tggcattagg acagtatatt gcgggaatgg gcttctcaaa tgtcggttta
    11761 ggactggtac acgggatggc tcacccgtta agtgcatggt acaacattcc tcatggcgta
    11821 gcgtgtgcag cacttttgcc aactgtcatg aaatataaca aagaatatac tggagaaaag
    11881 taccgtgaga tcgctcttgt tctagggatt aaaggagcag ctgaaatgtc tttagaagat
    11941 gtaagagaag cggcttgtgg tgagatcgat cggttgagca aagcggttgg aatcccagaa
    12001 accatttctg aattaggtgt aaaagaagca gatattccag caattgcaga agatgctttg
    12061 cgagatgtat gcacaccagg aaatccaaga gaaacgacag tagaagaaat cattgcttta
    12121 tatcaaagtt tgatgtaaaa acgaagcgga tcttcaacat taaattgcct gtaaagagaa
    12181 atggtagttt atatctggat atctctttta aattgatggt atttccgcta taatttttct
    12241 tataaagaaa taggagaata ttatatggaa aataaaaagc cttatggaac tgtattgatc
    12301 aaagcctcta aaatactaga ctatttagcg gaaaaccaag atgtctcttt gcagacgatc
    12361 gcaaagggtg tgggaatgac ctcttctaca gtcttgaaaa tactagatac tttgttgatg
    12421 atcggctacg taaataaaaa ttcagagaaa aactatcgtt taggtgcaaa gcttattcgc
    12481 tacgcgaata aaaacattga acaaatcgat ttggtagagt tgactttacc gtttttagaa
    12541 cagctgcagc aaaagatcga tgagactatc cacttagggg tattggacaa caatgagatc
    12601 ttgtacgtga acaaactaga tccaaaacat caaacgatcc gcatgtcttc aaaaatcgga
    12661 atcacccgac cattatacaa ttctgcgatg ggcaaagccg tattagcaga attcagtgaa
    12721 gagcagtaca ctgattatct tgaaaagcat ccgttgatcc cttacacaga atatacgatc
    12781 accaatgcat tacggttgaa aaaggaaatc caaacggtaa aagaaaccca catcgcattt
    12841 gacgatgaag aaatcgaacg tgatattttt tgtataggag cctcaatcgt gaaaaacgaa
    12901 caaatcattg gtgcatttag tgtaagtatg ccgaaatatc gtttaacaga tgagacaaaa
    12961 gaacaaataa tcagtgccct caagcaaaca aaaagcgaaa tcgagcaaag aattcaaaaa
    13021 taatgaaaaa agcgtttgca atttttcttt tctgccgtat aatatgggta tagatttcca
    13081 agttataaaa taaaatttct aaataggaaa taggtggaga agatgaaaaa aattgatatt
    13141 ttatctcgat tgaaaaacgc cggagtgatt gcggtggtac gcggaaaaag caaagaagaa
    13201 gctttgaatg cttgccacgc aatcatcaaa ggtggtctaa ctggtattga gttgactttt
    13261 acagtccctc aagcagatca agtaatcaaa gaacttttgt cattctacaa agaccaacca
    13321 gaaatagtga ttggcgcagg cacagtatta gacgctgtca cagctcgctt ggctatctta
    13381 gctggcgcag agtatattgt tagcccttcc tttgatcaag aaacggcaga gatgtgcaat
    13441 ctgtatcaaa ttccttactt accaggatgt atgactatta ccgagatcaa aacagcgctg
    13501 aaaagcgggg tagatatcgt caaactattt cctggaagcg cttatggacc aagtatcatt
    13561 tctgccttca aggcgccgat gcctcaagtc aatatcatgc caactggcgg tgtgagtctg
    13621 gacaatatga aagagtggtt cgatgctggt gtcgtaacag tcggagtagg tgggaacctt
    13681 ttagcacccg cagctacagg agattttgac aaagtaacgg aagtcgcgca acagtatgca
    13741 gcaaaaatga aagaaatcaa gaggtagctg cttatgggaa aagtagtgac actcggagaa
    13801 atcatgttgc gattgtccac agagcaggga acccgtatcg attcaagtga acgattcgct
    13861 gcccattatg gcggagggga agcaaacgta gcaatttctt tagctaatta cggacatgaa
    13921 gcgatttttg ctacaaaagt acctgataac gcattgggaa aagcagtcaa aaagcacttg
    13981 caatgttatg gagtagatac gaaacaggta ttatctggtg gatcaagact aggtacttat
    14041 tatatggaaa ctggtagtgg cgaacgcgcc gcctcagtga tctatgatcg tgccggatca
    14101 agttttgctg aaatggatca tttggaatgg tcgctagagg agttattcca aggaatagat
    14161 atatttcatg tttcaggaat caccccagct ttgtctaaaa aatggcaaaa aatgacgaaa
    14221 caggtgatag aagctgcaaa aaaagctggc tgcctgatca gcttcgatat caactatcgt
    14281 ggaaaattat ggacacagca agaagccagc gaagtaatcc atcagctttt acctttagta
    14341 gatatctgct cagctggaaa aatggatgct ctttatttgt taggtatcga gaaagcgcca
    14401 gaagcagaag aacaacctct aatttattat tatcaaaaaa tgcaggaaaa gttcccgaat
    14461 attcaggtgt tttattcgac caagcgtacc gttcattcag caagtgaaaa tgaattgaag
    14521 gggacattgt ggatgaacca tgaatatgtg gaatcccaag tccatagaat caccccaatc
    14581 gtggatcgag taggtggcgg agatgctttt gcaggtggcg tgctccatgg gattttatcc
    14641 aatatgtctc cgcaagaaat cattgatttt gctacggcag cttctgcctt gaagcatacg
    14701 atccatgggg actgcaacca atttagtcaa gaagaagtcg cacaattttt agcatgcggc
    14761 tctggaaaaa tcattcgata gaaggagtag aagaaaatgc aagaaatgga aacacgttat
    14821 acacacagtc cagaagatat ccgtcattat tcaacggaac aattgagaaa agaattttta
    14881 gtagaaaagg tatttgaacc agggaaaatc agtttgactt atacgcacaa cgatcgaatg
    14941 atctttggtg gagtgacacc tactgaaaaa gaattagaaa tcacattaga caaagaacta
    15001 ggtgttgatt acttcttaga gcgtcgtgaa ctaggtgtga tcaatatcgg tggaccagga
    15061 ttcatcgaga tcgatgggca aaaagaagaa atgaaaaaac aagacggata ctatatcgga
    15121 aaagaaacac gacatgttgt attttcttct caagatgctg aaaacccagc taaattctat
    15181 attagttctg ttccagctca tcataaatat ccaaatgtaa aaatcagtat cgaccaagta
    15241 aaaccaatgg agactggtga aggcctgaca ctgaatgaac gtaaaatcta ccaatacatc
    15301 catccaaatg tatgcgaaag ctgtcaatta caaatgggtt atacgatttt agaaccagga
    15361 agcgcttgga atacgatgcc ttgtcataca catgaacgtc gtatggaagc ttatgtctac
    15421 tttgattatg ccaatgaaga tacacgtgtg ttccacatga tgggcaaacc agatgaaaca
    15481 aaacatttag ttgtagacaa tgaacaagcc attatttcgc caagctggtc gatccattca
    15541 ggtgtaggta caagcaatta ctcattcatc tgggcaatgt gcggagaaaa tatcacctat
    15601 acagacatgg atatggtgca aatggatcaa ttaaaataag cataagggag agaaaatgat
    15661 ggaatttagt atggatatgt ttcgtttaga tggaaaagta gcattagtca ccggtgctgt
    15721 ctatggtatt ggatttgaga ttgcacgctc tttagctaca gcaggagcaa aaatcgtatt
    15781 taataacttg aatcaagaat cagttgatga aggtatagcg cattataaag aagcaggcat
    15841 cgatgcaaaa ggttatgtct gcgatgtgac cgatgaagaa gcggttcaag cgatggttca
    15901 acagatcaaa gaagatgtag gttcagttga tattctagtc aacaatgcag ggatcatcaa
    15961 acggacaccg atgatcgaaa tggatgcggc agacttccgc caagtaatcg acgtggattt
    16021 aaatgcccca ttcatcgttt caaaagcagt tattccggat atgatcgaaa aaggcggcgg
    16081 taaaatcatc aatatctgct ccatgatgag tgaattagga cgcgaaacgg tcagtgctta
    16141 tgctgcggca aaaggcggat taaaaatgct gacgaaaaac atcgcttctg aatacggtca
    16201 atacaatatc caatgtaatg gaattggacc aggttatatt gctacaccgc aaactgctcc
    16261 attacgcgaa acacaagaaa acggtgaacg tcatccattc gatcaattta tcgttggacg
    16321 tacgccagca gcacgctggg gcgagccaat cgatttggct ggaccatctg tcttcttggc
    16381 ttctagcgca tctgatttcg ttaacggcca tgtcttgtat gtggacggag gcatacttgc
    16441 ctatatcgga aaacaacctt aaaagagctt tagtacactg tttagtggag aggttgtgaa
    16501 atcagcggat tgaacgaata ccgaaggaca gctgattaaa catcatttat tcgaaaaaag
    16561 aggctgggac agaagc
//
LOCUS       DACENJ010000043        16537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.043, whole genome shotgun sequence.
ACCESSION   DACENJ010000043 DACENJ010000000
VERSION     DACENJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.043"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..1143
                     /locus_tag="IW748_001036"
     CDS             4..1143
                     /locus_tag="IW748_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1103957.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1136..1936
                     /locus_tag="IW748_001037"
     CDS             1136..1936
                     /locus_tag="IW748_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1103958.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1936..2763
                     /locus_tag="IW748_001038"
     CDS             1936..2763
                     /locus_tag="IW748_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1103959.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2741..3475
                     /locus_tag="IW748_001039"
     CDS             2741..3475
                     /locus_tag="IW748_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1103960.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3575..4441
                     /gene="rfbA"
                     /locus_tag="IW748_001040"
     CDS             3575..4441
                     /gene="rfbA"
                     /locus_tag="IW748_001040"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1103961.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4455..5027
                     /gene="rfbC"
                     /locus_tag="IW748_001041"
     CDS             4455..5027
                     /gene="rfbC"
                     /locus_tag="IW748_001041"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1103962.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5049..6077
                     /gene="rfbB"
                     /locus_tag="IW748_001042"
     CDS             5049..6077
                     /gene="rfbB"
                     /locus_tag="IW748_001042"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1103963.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6175..7026
                     /gene="rfbD"
                     /locus_tag="IW748_001043"
     CDS             6175..7026
                     /gene="rfbD"
                     /locus_tag="IW748_001043"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1103964.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7060..9093
                     /locus_tag="IW748_001044"
     CDS             7060..9093
                     /locus_tag="IW748_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103965.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9137..10416
                     /locus_tag="IW748_001045"
                     /pseudo
     CDS             9137..10416
                     /locus_tag="IW748_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            10626..11432
                     /locus_tag="IW748_001046"
     CDS             10626..11432
                     /locus_tag="IW748_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1103966.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11444..12664
                     /locus_tag="IW748_001047"
     CDS             11444..12664
                     /locus_tag="IW748_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1103967.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12654..14240
                     /locus_tag="IW748_001048"
     CDS             12654..14240
                     /locus_tag="IW748_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1103968.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14279..16417
                     /locus_tag="IW748_001049"
     CDS             14279..16417
                     /locus_tag="IW748_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1103969.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         5249 a         2741 c         3539 g         5008 t
ORIGIN      
        1 catatgcttg tttcttttag tatggttata agattttttc tgacaatttt attatcactg
       61 atattaacac ctgtattcaa aatcatttca gtacaaacag gtatggtcga caaaccaaat
      121 gaaagaagaa taaataaagt tccaatgcct tcagcgggcg gactgcctat ctttatttca
      181 tttgttatat cgactttatt cttattccgt aaaattatcc ctcaatcgta tattgtaccg
      241 atcttgattg cttctttagt aatcatagtc acaggagtgc tcgatgataa atatgagctg
      301 acacctaaac aaaaaagtat agggatcttg attgcttctc ttattattta ttttgtagca
      361 gatatacgta ttgattcctt cacgcttcct tttattggat atatccaact aggctggctt
      421 agttttcccg taacgatttt ttggatcttc ggtatcacga atgcggtcaa tctgattgac
      481 ggtttggatg ggctggcggc agggatttca ttgatcggtt taattacgat aggaatcatt
      541 ggatattttt tcctccatgc ttctacagtg tatattccag ttgttatttt ctgtcttgta
      601 gcaagtatta tcggtttttt tccttacaat ttttatccgg caaagattta tttaggcgat
      661 acaggagctc tattcctagg gtttatgatg gccgtccttt cactacaagg gctgaagaac
      721 gtgacttttg tttcatcgat ttctttgctg atcgtcatgg gagtgccggt aacagacacg
      781 ttttttgcta tcattaggag aaaagcaaac cgtgtttctt tttctacagc agataaaaaa
      841 catcttcatc accgactaat cagtttaggc tttactcata aaggtgctgt cctgacgatt
      901 tatgcgatgg ctttgatgtt ttctttcaca gctatggtca tgaattatac tggaagaatc
      961 ggaacgatca tattgattat tgccatgttg tttgctgcaa ttttattatt tgaattgatt
     1021 ggtctgatca atgagaaaca tcagtttatt ttaaaaacat tgcgattttt agggaataaa
     1081 gagtaccgta cgcaagtaaa acagaaatat ttgaagaaat ggaaccgtca tatagatgaa
     1141 taacaaatta aaccagccga tccttattag tattgtgacg gcaaacagtg ataaaatttt
     1201 tcggacacta gataatttta tttcaactgt aaaagatgaa acagcagtaa aaatccgaat
     1261 ttttgacaac aattcacttc ccgagtacaa aaaaagacta acagagtata ggaaatatcc
     1321 ctacatcgat atctattttc atgatgaaaa ttccggattt ggttatggac ataatcacaa
     1381 tctgatagct agcgattctc gctatggcat tatttgtaac ccagatattt tagtggatga
     1441 agagacagtt acagcattgg tcttgttatt gaaagaacat ccggaatgtg cgatgttagc
     1501 acctaagatt cttaatgaag atggaacaac tcagcacctc atccgtgagc agctggctgt
     1561 ttttgattac gttttgcgct ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg
     1621 actggctgtg tttgaatgcc gaggattgcc tactgatgct aatagttatg tacggatgat
     1681 ctcaggtagt tttatggtag ctgatattga taaatttaaa gagataaagg ggtttgatga
     1741 gcgtttcttc atgtactttg aagacaacga cttgtgtttg agttttgaaa aagcaggtta
     1801 caagttattg tacacaccct tttatgcagt tatccatttt tacgggaagg gtgcgcatcg
     1861 aaatgcaaaa ctgtttcgcg tgtttttgac ttctatgatg aaatttttca ataagtgggg
     1921 gtggcgtttt ttctgatgaa accagaaata ttagtagtcg ttgtgcttta tcagcaacta
     1981 ttcagtcaat ctccagctta tggaccatta agcaaagcac ttagcgaaaa gcaaattcag
     2041 cttattattt acgataatag tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat
     2101 atcgtttatc atcatgatcc gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg
     2161 tctcaagcag atgaaggaac acgttgtctc gtcactctgg atcaggatac gaggctagtc
     2221 gacgattatt ttgaactatt gcgtaaagta acctttacag atgaatgtgt tgctgcagtt
     2281 ccgatgattt tttctgggag ccgacaagtt tctcccctat attcagatcg ttatatcaat
     2341 cgtacagctc aagcagtaga agttgaaggc ccaacagctg aacgaatcat ggcaatcaat
     2401 tcaggtgttg cctggtctgt caaatttcta aaaaagattg gcgggtttaa tcctgctttt
     2461 tctttagatt ttttggatca ttggctgttt tggaaagtgg atcagttgga aaaaactgtg
     2521 gagatccttc ctgtacgaat ggaacatgat ctttcggttt tagactatga aaaagtttca
     2581 agtaatcgat accgttctat tttacaagca gaaaatcgat tttatcatga atatgatcaa
     2641 aggcatttat ccagtcaccg acgacaatta ttgttaagaa cagctaaaca gttcttgacc
     2701 gtcaaaaacc ggcatatctg gcggatgact ttgcgctcat atgttaagaa ttggaaggtg
     2761 tgaattcgtg cgttcggttt gtatagctac ctttaatgga ggcagatatt taagagagca
     2821 gttagatagt attttatctc agctttcagc agaagatgaa gtagtcattt ctgatgatgg
     2881 gtcagtagat gatacatggg atatattaac tgaatacgca gaaaaagata agcgaatcca
     2941 gcttttttcg gggcctcgtc aaggattgat cgctaatttt ggttatgcga tccagcagac
     3001 gaaaggagat ctaatctttt tggcagacca agatgatgtt tggttggaga caaaagtgaa
     3061 aaagatcact gactattttg agcagaatcc ggatcagcta gtagtagttt ctgatttaat
     3121 cattgtggac agcgagctgc aagagatcca tgattcgtat ttttcgtttc gaaattccaa
     3181 agaaggatgg cttaataatg tgataagaag tcactatatt ggtgctggta tggctttccg
     3241 gtcttcttta aaacccgata ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg
     3301 gattggtttg ctgggaggaa agaagactgg gttcctaaga gaaccgttaa ctctgtaccg
     3361 aagacatgat ttaaacgcga gtgagattga aacacattct aaattacggc aaaaaatttc
     3421 ttggcgtgtt catctgatta cggcgttgat caaacgaaaa tggtttaaac gttgataaaa
     3481 gatgtttaaa tccttaatat ttccatgaca aatataacaa ttgatgagat aataacaaag
     3541 tgaaatttga aatcaaaaag gagagcagat atccatgaag ggaatcattc ttgctggggg
     3601 cagcggaaca cgtctgtacc ctttaacaaa ggcaacatcg aaacaattga tgccgattta
     3661 tgacaaacca atgatttatt atccaatgtc tactctgatg ttggcgggaa tcaaagaaat
     3721 tctgattatt tcaacgccac aagatacacc acgtttcaag gaattatttg gaaacggaaa
     3781 tgatttagga ctgcagatcg aatatgcagt acaagaaagt ccagatggat tggcacaagc
     3841 gtttatcatt ggagaagaat ttattggaga tgatagtgtc tgcttggtat taggggacaa
     3901 tatttactat ggtggcggtc tgtctaaaat gttgcaacga gcagcttcaa aagatacagg
     3961 cgcgacagta tttggctatc atgtcaatga tccagaacgc tttggtgtag tagaatttga
     4021 tgagaatatg caagctttgt cgattgaaga aaagcctgct cagccaaaat caaattacgc
     4081 tgtaactggc ctttattttt atgacaatga ggtaatttct atcgctaaag gtatcaaacc
     4141 ttcagaacgt ggagaactag agattacgga tgtcaataaa gcatatttgg ataaaggaaa
     4201 actatccgta gaacttatgg gacgcggatt tgcttggctt gatacaggaa cacatgaatc
     4261 attgcttgaa gcatctacct ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc
     4321 ttgtttggaa gaaattgctt accgtatggg ctacatcgat aaggaacaat tgctggccct
     4381 tgcacagcca ttgaaaaaaa atcaatacgg tcaatattta ttgaatttgg ccgctgaata
     4441 aggagtcctt acagatgaaa gtaacagata caaaattaac agatgtcaaa atcgtagaaa
     4501 tggacgtctt tggcgatcat cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg
     4561 cagaacatgg attgaaatat gactttgttc aagacaacca ttcactatca gctgaagctg
     4621 gtgtccttcg ggggcttcat ttccaaaaag gagaagctgc acagacaaaa ttgattcggg
     4681 tagtcactgg agcagtttta gatgtagtag tggatatccg caaaggtagc ccgacttatg
     4741 gtcaatggga cggatttatc ttatctgaac ataatcatcg acagttgctt gtacctaaag
     4801 gatatgccca tggattcgtt actttgacac caaatgtgaa cttcctttat aagtgtgata
     4861 attactataa tgcagaagca gatgggggca tcgcatttga tgatccagat ttagcgattg
     4921 attggccaat tgatccatca agagcgatta tgtcggaaaa agatcaccgt catccgacat
     4981 taaaagaatt tgaagcagaa aatccatttg tttacggaga aatctaagaa ttaaggagaa
     5041 attttcttat gaaaaacatc attgttaccg gaggtgccgg attcatcggc tctaatttcg
     5101 ttcattacgt ggtaaacaat catccagaag tgcatgtcac tgttttggat aaactgactt
     5161 atgcaggaaa caaagagaac ttggctggac ttcctagcga tcgcgtagaa ttggtcgtag
     5221 gagatgttgc agatgcagaa ttagtggacc gtctagtaaa agaagcggac gcagttgttc
     5281 attacgcggc agaatcccat aacgataatt cattaaaaga tcctttccca tttgtacaaa
     5341 cgaatatcat tggtacgtat acacttttag aagcttgccg caagtatgat gtacgctatc
     5401 atcacgtatc tactgacgag gtttacggcg atcttccatt acgtgaagac ctgccaggac
     5461 atggggaagg cgaaggagaa aaattcactg cggaaacacc atataatcct tcaagtccat
     5521 attcttctac aaaagctggt tcagatctgt tagtaaaagc atgggtgcgt tcttttggct
     5581 tacgtgcaac catttcaaac tgttcaaaca actatggtcc ttatcagcat attgaaaaat
     5641 ttattcctcg ccaagtaacg aatatcttaa gcggtattcg tccaaaatta tatggcgaag
     5701 gaaagaatgt tcgtgactgg atccatacga atgatcattc ttcagcagtg tggctgattt
     5761 tgacgaaagg cagaattggc gaaacctatc tgatcggtgc agacggtgaa gaagataata
     5821 agaccgtgat ggaattgatc ttagaaatga tgggtcaacc aaaagatgcg tacgaccatg
     5881 taaacgaccg tgcaggacac gacttgcgtt atgcaatcga ttcgacaaaa ttacgggaag
     5941 agttaggatg gaagcctgag tttacgaact tccgtgatgg gttggctgac acgatcaaat
     6001 ggtacgaaga acatgaagac tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg
     6061 ccaaaaatgg acaataatac agagaaaatg tcactgtatt aatggaaaaa cacatgtgag
     6121 gcaggcgctc gcctgtctct tttttaaaat aaaaatcaag gagcggataa tcatatgttt
     6181 ttattaactg gaggaaatgg gcaactaggt acagaattgc gccatttatt agatgaaaaa
     6241 aatctgcaat atgtttcaac agatgcaaaa gacttggata tcacagatgc cgagaaaaca
     6301 atggcttaca tcacagaatt aaaacctgat gttattttcc attgtgcagc ttacacagca
     6361 gtagataaag cagaagaaga agcaaaagaa ttagatgaga agatcaatgt tgatggtaca
     6421 agaaatgtag cacttgcagc aaaagctgtg aatgctcaat tcgtctatat cagtacagac
     6481 tatgttttcg atggaaaaaa caaaaatgta gaatacgaag tagacgacca aacgaatcca
     6541 ctaaatgaat atggtcgtac gaaattatta ggcgaaaaag cagtagaaga gattttagaa
     6601 gactattata tcatacgaac ttcttgggtt ttcgggatct atggacacaa tttcgtcttc
     6661 acgatgcaac gcctagcgga aacccgcgac aaactgactg tagtaaatga ccagtttggg
     6721 cgaccaactt ggacacgtac tttggcagaa tttatggtat atgtcatcga agaaaaagca
     6781 ccatttggta tttatcattt gtcgaacgaa aatagctgtt cttggtatga attcgcaaaa
     6841 gaaatattga aagatacaga tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa
     6901 aaagcaacac gtccgcagta ttcagtcatg agcctgaaaa agacagaaga gttaggattt
     6961 gtcataccaa cttggcaaga agcacttgcg caaatgctgg aagctgccaa aaaaatgaat
     7021 aagtaatttc cttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt
     7081 gcacgacgaa aagaaaagcc aaaagcgaac caggcagatt cgaatttggt gcttgacctg
     7141 tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat
     7201 ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa
     7261 aaaactgatg tgatttttgg acagaatcgg gaagtgcggt cagacgaatg gctggtgcag
     7321 acaccttttt acctctcaca agctgaagag gactttccgt tggtgaacga ggattattca
     7381 ttaaacggac aaaacatgat cattgcgtat aattctcctg taaaggatat tacggttatc
     7441 ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat
     7501 tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg
     7561 aagagaaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc
     7621 cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgact
     7681 gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt
     7741 ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg
     7801 ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt
     7861 tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc
     7921 atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat
     7981 ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt
     8041 acaaattgga aaattccttt tgaagatatc aactattcga ataactctga agtggccctg
     8101 ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa
     8161 gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt
     8221 tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct
     8281 ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc
     8341 gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta
     8401 aatgcatggt cttatttcta tgcgattacg cattcgaaaa tggataatta tttttcggat
     8461 tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcttttgc attatttggc
     8521 tggagacgct tgttttacct cgcttttgcc gccttagtta tcgtatctgg ctttttcgtc
     8581 aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa
     8641 aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc
     8701 accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca
     8761 tggaagaaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca
     8821 agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg
     8881 gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga
     8941 ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat
     9001 aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca
     9061 caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat
     9121 gttagtagaa aggagagtgg agataattca agcaatcgta caaaaatttg tcaacaaaat
     9181 gagtgtcggt ttcttgcttt tgttactgac gctagtcttt tttgtttgga cagcagggtt
     9241 aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt
     9301 ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg
     9361 tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt
     9421 atttggagac ttactgtttc ctttgattat tttattttgt ttagtaatta aatggatcga
     9481 gtggacaaaa tgggatcgcg gtatttatct gattgtcttt tcggtcttcc tcggaatcac
     9541 gtttatcgtg tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc
     9601 aattcgcttt ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt
     9661 gctttattga tcagtggatt tgccagttct tttgaacgat ggtatgtacg aatcatcggt
     9721 gcagtagcag ttgttgcagc ttttctcggg atttgggtct gccaatctag aggtgcatta
     9781 gtcgcgttga ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt
     9841 cgtgcgccat ttattgctta cacggttacg attcttttag ctttgccgat ttcttatctg
     9901 gcggctgtct ctgaaaaagt caatttgttt accggtcgag aagatatttg gcataaattc
     9961 tatcagacgc taggagaaaa atctgaacaa attttactag gaatgaaaac atttatcttc
    10021 caacgaggaa accaattttt aggaaaccac aatagttata attctatttt gaatatctat
    10081 ggattgatag gatttgggat tgctgctttg ctgcttatcc tttttattgg tcgcttaacg
    10141 ttgaaagcag atttatccaa cgggcagatg acttttatct gggccttttt tgcggtgatg
    10201 atgcagtcat ttatggagga tacattgact tcataaccat gggttccgat tatttatatc
    10261 ctgttggcta tggccgttca tcgatatgat gaaccagaag ttgcggagga attctatgtt
    10321 atggaagagg catatgacga tatgacttat gatgatacat cttatgagga acaaccagat
    10381 agcgctcatt cttccaggat ccaacgctat cattgatagg cacaaaatac gagagaggct
    10441 gtgacaaccg tcgcagcctt ttttggttta ttgcaattgt ctagcatctt ctgtaaaata
    10501 gaaacgttag agaaaatctt gtatcataga aaattaagaa agaaataaga acgcagctcg
    10561 gtaccaaaaa tatggtaaaa tggcacttga ctgtaattca ctgaaaatcg cttggaggaa
    10621 cacaaatgtt taaagatttg tatttattta tgaaggacat ttatcagaac agaaaattat
    10681 tgctacagtt ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct
    10741 tatgggcgtt tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg
    10801 gactgaaagc acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga
    10861 tgattccctg gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat
    10921 atacttattt agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc
    10981 tatccaatct gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa
    11041 atggtatata tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg
    11101 ctttcttgac aggattgact tggctgacgg catcgatcca accattttta cctgatatca
    11161 tgcaattaat tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc
    11221 ctaatcagtt tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg
    11281 ttcaaggtta tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata
    11341 ctttgtattt ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta
    11401 gattgaaacc acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat
    11461 tagactaaaa gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg
    11521 tgaagccctc aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt
    11581 gaatgtagaa ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa
    11641 atctacaatg ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat
    11701 cgaaggaaaa atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg
    11761 ctatgaaaat atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga
    11821 aatggtcgat gatatcattg aatttgctga catcggagac catatttatc aacctgtaaa
    11881 aacttattca agcgggatgt tcgttcgtct agcttttgct gtagcaatca atgtgaatcc
    11941 cgatattttg attgtggatg aagcattggc agtaggcgat cttgaatttc aactaaaatg
    12001 tatggaaaaa tttacagaaa tcaagaactc gggaaagacg attttattcg tttctcatga
    12061 tatcagttcg attcgtcgtt tttgtgatcg ttcctattgg ttgaaaaacg gagaagtcgt
    12121 cgaatatggc gatacgatgg aagtaacgac gaattatgaa aactttttaa agaaaaaatc
    12181 agttaaaaca gtggatcgga ataaaaacca agtagaacgg ttcgccgctc cagatatcgt
    12241 cgacgtgacg aaagcggaat tgttggataa agacaaaaat ccggtggata tgctggaaca
    12301 aggagaaaaa ctatacgtta aagtaactta tgaagtaaaa gatgatacaa tcaaaaatcc
    12361 ggttttagga attgcgctac gaacagtcga caatttttat gtatgcggac ttaatactct
    12421 actagatggt atcaagattc cttggaaaaa aggagagaat atcttttatc tggagtatga
    12481 aaagttaggt ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc
    12541 aacggttcct ttggtatata agacaaagta catgaacttg tttgtcagcg gttcttatat
    12601 cggagaaggc atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat
    12661 atgattttga aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca
    12721 aaccaggaag taaagtttta gagtttggtc ccggaaacgg ccgattaacg aaacatttaa
    12781 tcggagcaaa acaatgtgaa gtgagcattg ttgagttaga taaggaattg ttcgattttg
    12841 tcagcgaatt tgctcaagat ggattttatg gtgatatcga aagttttgag tgggcaaact
    12901 attatgctgg acagacattt gattacgtct tgtttgcgga tgttttagaa catttggtag
    12961 atcctggaaa aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga
    13021 ttacattccc taacttggcg cataattctg tcatgatcga tttgttcaat aaccaattgc
    13081 cgtgggcttc ttatggtctc ctagatgaaa cccataattc tttttatact catgatggct
    13141 tccaaaaagt ttttgaaaaa gcgggattat tcattaatat tgaagattat ttgtacttag
    13201 ctgtaggtga tacagaatta aaatcaactt atgaagaatt gccggaagct gtacgctatg
    13261 atttcaaaat gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc
    13321 cagtagcaca aagttccatt gcagagccac aaaattctaa ttatgtaaaa gtgttagaag
    13381 taacccagca aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag
    13441 gggaaaatga gacactaagt ttccccgtat cagaaacaac agaaagaatg gtgtttcgct
    13501 tcgcgcaaca acctagcttt atcgaattca gcgcagaagc tgccggagaa aaactaacat
    13561 ttatcgattc gaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag
    13621 aattaccaga gttcgttcta acggatattt ctgggaaaga agtgactatc cattgtcact
    13681 atcgctttat cggggaattg acaccaacaa tgaaagaatt gttggaaacg attcgtccca
    13741 tggctgaagt cacaaagcag ctttctgaga aaaatgacga attagaaaga gaaaatcatc
    13801 atcttcttga ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt
    13861 gcacattgtt ggaaagtgat gaatggacaa taaaacatag actaggacga cgtaagaaag
    13921 aaacttcgaa aaaaatccaa gaaaaagaac tttctatatg tattgacgag aaaatctggg
    13981 atgcagaaac aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc
    14041 ccttgtccta taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac
    14101 gagaagaagt caatcaagca gaacagctcg caaaaggaac agaagcggga tttgagttac
    14161 gaatcctttg cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggccaat
    14221 catggatcat tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt
    14281 gcaaaaaaaa gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga
    14341 tttgcttatt gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt
    14401 agaaaaagaa aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt
    14461 atataatctt gatgttaaga cccaaagcgg ttttaaggta cgtttatcag ggaaaatgag
    14521 aggaaaagcc atcttagact tccaaacagc gagacatcaa aatggcatag ctgtgaaatt
    14581 aaacggtaaa tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt
    14641 actgaaaaaa gggataaatt atgcaaggac tcatggcgta aaaaaagcga tccgtcgggt
    14701 caaattggaa ttgaagccag gatcgatcga ctatgcagag tggatcagtc gtcatgagaa
    14761 accagatttg aaagagcaaa ggaaagaagc acaaggattt gcgtaccgtc cattgatttc
    14821 catacttatg cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt
    14881 actggatcaa acgtatgatc attgggaact atgtatcagt gatgatgctt ctactgatcc
    14941 agctatccga aagtgtctgg aatcctatca agcaaaagat gatagaatca aagtcgtttt
    15001 ccgaaaagaa aatggacaca tcagtctggc aactaattct gcattggaaa tagcagaagg
    15061 agaatttata gcactcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt
    15121 ggcaaaagtg ctaaacgtac atccagaact ggatgtgatc tacagtgacg aagataaaat
    15181 cgatgcagat ggcaatcgct ttgatccgca tttcaaagca gactggtcgc ctgatacatt
    15241 gatgggaaat aattatatct ctcacttagg cgtatatcga acaagcattg ttaaagaact
    15301 tggcggtttc cgtaaaggct atgaaggttc tcaagattat gatttggtcc ttcgtgtgac
    15361 agaacaaatc cctgcagatc atatttacca cattgaccga gtcttgtatc attggcggac
    15421 gatcccagga tcaactgcaa gtaatggcga agccaaaagt tatatctatg attccggagt
    15481 gaaagcattg acagatgccc tgtctagaag aaacatcaaa ggaagcgtac gtcctggcag
    15541 aatttctgga ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagtgtgat
    15601 cattccaacg aagaatggct atgaagatct aaaaacttgt gtggattcga tcatcgaaaa
    15661 aacgtcttat tcaaattatg aaatcattat tgcggataac ggaagtacag atccaaaaat
    15721 gcaagaatta tttgcagaat acaagcatca gctgaaagac cgctttatcg tagaattgat
    15781 tgacattcca ttcaattatt ctcgaattaa caatttagca gcagaaaaag caaacggcaa
    15841 atacttttta ttcttgaata acgatacaga agtgattgag ccagattgga tgacggtgat
    15901 ggtttcttat gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga
    15961 tgatacaacg cagcatgcag gagtattatt aggaatcggt ggtgttgcag ggcatgcatt
    16021 gaataactat gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta
    16081 cttagcagtc actgcagctt gtatgatggt caaggcagct gacttcaatg ctgttaacgg
    16141 gtttgatgaa acattagaag tagcattcaa cgatgttgac ttgtgcttga aagtttatga
    16201 actgggtcgt tacaatgtct atgcacatca agcagaattg taccatttcg aatctaaatc
    16261 aagaggttat gaagatactc cagaaaaaca aagacgtttt gctggtgaaa tcaaaaaaat
    16321 gcaagataaa tggccagcat atattgcgca tgatccattt tacaatgata atttaacaaa
    16381 agaaggaatc ggcgactttt cgttgagacc agattaaact ataataaaaa gctgttatgt
    16441 tgtgattcgc tcgcacataa cggcttttta tagttttttg tcaaaaaaag gcagaaggtg
    16501 ccgactcaaa aaaataagtc gaggttggga cagaagt
//
LOCUS       DACENJ010000044        16490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.044, whole genome shotgun sequence.
ACCESSION   DACENJ010000044 DACENJ010000000
VERSION     DACENJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.044"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..2706)
                     /locus_tag="IW748_001050"
     CDS             complement(1..2706)
                     /locus_tag="IW748_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1103970.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3012..3959)
                     /locus_tag="IW748_001051"
     CDS             complement(3012..3959)
                     /locus_tag="IW748_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1103971.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4149..4418
                     /gene="rpsN"
                     /locus_tag="IW748_001052"
     CDS             4149..4418
                     /gene="rpsN"
                     /locus_tag="IW748_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1103972.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4594..4956)
                     /locus_tag="IW748_001053"
     CDS             complement(4594..4956)
                     /locus_tag="IW748_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1103973.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(4967..6088)
                     /locus_tag="IW748_001054"
     CDS             complement(4967..6088)
                     /locus_tag="IW748_001054"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1103974.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6104..6454)
                     /locus_tag="IW748_001055"
     CDS             complement(6104..6454)
                     /locus_tag="IW748_001055"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1103975.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(6584..8095)
                     /locus_tag="IW748_001056"
     CDS             complement(6584..8095)
                     /locus_tag="IW748_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1103976.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8417..9769)
                     /locus_tag="IW748_001057"
     CDS             complement(8417..9769)
                     /locus_tag="IW748_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1103977.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(9922..10998)
                     /locus_tag="IW748_001058"
     CDS             complement(9922..10998)
                     /locus_tag="IW748_001058"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1103978.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11194..12939
                     /locus_tag="IW748_001059"
     CDS             11194..12939
                     /locus_tag="IW748_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1103979.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13219..13656)
                     /locus_tag="IW748_001060"
     CDS             complement(13219..13656)
                     /locus_tag="IW748_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1103980.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(13640..15013)
                     /locus_tag="IW748_001061"
     CDS             complement(13640..15013)
                     /locus_tag="IW748_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1103981.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15335..15823)
                     /locus_tag="IW748_001062"
     CDS             complement(15335..15823)
                     /locus_tag="IW748_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1103982.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(15845..16447)
                     /locus_tag="IW748_001063"
     CDS             complement(15845..16447)
                     /locus_tag="IW748_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1103983.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4626 a         3532 c         3008 g         5324 t
ORIGIN      
        1 ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat
       61 gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca
      121 gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac
      181 cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg agacttggat
      241 cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt
      301 accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat
      361 caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat
      421 attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg
      481 agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc
      541 tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa
      601 cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac
      661 aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc
      721 gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc
      781 tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac
      841 ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg
      901 ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc
      961 attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt
     1021 cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt
     1081 ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa
     1141 gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg
     1201 gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc
     1261 attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc
     1321 ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg
     1381 ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg
     1441 caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc
     1501 aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc
     1561 ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat
     1621 tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga
     1681 ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc
     1741 tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc
     1801 ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc
     1861 ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt
     1921 caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg
     1981 gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact
     2041 ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga
     2101 taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat
     2161 ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg
     2221 cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc
     2281 atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc
     2341 ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc
     2401 ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga
     2461 ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc
     2521 actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata atgctttgat
     2581 gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat
     2641 cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga
     2701 ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac
     2761 ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct
     2821 cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga
     2881 aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa
     2941 agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat
     3001 tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca
     3061 actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa
     3121 acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga
     3181 tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga
     3241 tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt
     3301 tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc
     3361 agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt
     3421 tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg
     3481 atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg
     3541 atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct
     3601 gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact
     3661 tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt
     3721 gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg
     3781 aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc
     3841 acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca
     3901 tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg
     3961 gtcaccatct ccctttttct ttaagtttac gtttatgtct ttattttgac aaaaaaaatg
     4021 tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt
     4081 aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga
     4141 atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg
     4201 aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta
     4261 aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc
     4321 ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata
     4381 aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa
     4441 aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta
     4501 gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt
     4561 atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc
     4621 gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg
     4681 gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac
     4741 ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg
     4801 tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag
     4861 attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga
     4921 aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt
     4981 ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat
     5041 ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg
     5101 ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa
     5161 tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc
     5221 catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt
     5281 ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca
     5341 gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc
     5401 cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg
     5461 ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta
     5521 taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat
     5581 gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt
     5641 ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa
     5701 tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt
     5761 cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag
     5821 gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt
     5881 catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt
     5941 caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg
     6001 gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt
     6061 tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa
     6121 tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag
     6181 gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg
     6241 taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc
     6301 cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag
     6361 aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa
     6421 gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta
     6481 gaacactctc attctaccaa attataacca ttttgccaca aataagtcca caaataacaa
     6541 aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac
     6601 ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat
     6661 tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat
     6721 tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca
     6781 atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt
     6841 ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt
     6901 gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac
     6961 ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt
     7021 tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag
     7081 acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat
     7141 cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag
     7201 caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca
     7261 ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta
     7321 atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag
     7381 gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga
     7441 catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg
     7501 gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa
     7561 gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc
     7621 ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacagtt ccaagtttca
     7681 atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac
     7741 gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac
     7801 gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac
     7861 gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca
     7921 ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt
     7981 ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag
     8041 cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa
     8101 tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg
     8161 gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc
     8221 ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc
     8281 gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac
     8341 cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct
     8401 gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca aataagccca
     8461 tgctgttgct gccttttagc ataatactat ctgttgctgc aagattttgt ttgatagcat
     8521 caatcaattt attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt
     8581 tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct
     8641 caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca
     8701 gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta
     8761 ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat
     8821 ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc
     8881 gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg
     8941 ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg
     9001 tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg
     9061 ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca
     9121 caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc
     9181 cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt
     9241 ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca
     9301 aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat
     9361 tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta
     9421 gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt
     9481 tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa
     9541 ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt
     9601 ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata
     9661 aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag
     9721 agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc
     9781 ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt
     9841 ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc
     9901 cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta
     9961 tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt
    10021 ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt
    10081 caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc
    10141 taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac
    10201 ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa
    10261 tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac
    10321 agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg
    10381 ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac
    10441 actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg
    10501 atcaaatact tttttaggat tttctttcca ttgattctta agtactggta cataaggaac
    10561 ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc
    10621 actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat
    10681 cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt
    10741 gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc
    10801 ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgac
    10861 ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc
    10921 atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata
    10981 tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc
    11041 tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc
    11101 ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat
    11161 aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc
    11221 ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa
    11281 aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata
    11341 ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa
    11401 acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca
    11461 gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct
    11521 gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag
    11581 atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg
    11641 atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa
    11701 gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa
    11761 caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct
    11821 ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta
    11881 atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa
    11941 caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt
    12001 ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag
    12061 acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt
    12121 cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt
    12181 ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg
    12241 ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa
    12301 ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat
    12361 gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt
    12421 catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca
    12481 ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct
    12541 tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga
    12601 ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta
    12661 ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa
    12721 ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca
    12781 ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt
    12841 tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc
    12901 gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga
    12961 atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa
    13021 ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag
    13081 ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt
    13141 ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc
    13201 ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg
    13261 atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc
    13321 actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga
    13381 gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt
    13441 gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc
    13501 gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac
    13561 actggtcacg ataaccacgt caagaccata ggattctcct aattttatga catcttcttt
    13621 tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat
    13681 gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa
    13741 gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga
    13801 ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga
    13861 tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag
    13921 ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac
    13981 taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac
    14041 tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc
    14101 cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt
    14161 tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta
    14221 gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct
    14281 ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat
    14341 caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga
    14401 atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat
    14461 ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat
    14521 aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga
    14581 gaaatagcca gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg
    14641 tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta
    14701 gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg
    14761 aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc
    14821 gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca
    14881 aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa
    14941 taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga
    15001 gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt
    15061 tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag
    15121 aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag cagacaagcc
    15181 cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa gatagaaatc
    15241 aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc ttctcgaccc
    15301 gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt ttcattttat
    15361 aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta atctcaaatc
    15421 cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc aaaaagatct
    15481 ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa gaagtcaatc
    15541 cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt ccatttatct
    15601 cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag taggacacac
    15661 cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc atgtaatcca
    15721 cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt atgactgcca
    15781 tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca cgtccttttt
    15841 tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg ctatacacac
    15901 ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa gataatttcg
    15961 tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa
    16021 ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact
    16081 tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca agcaaaaatc
    16141 cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat ccattgccaa
    16201 acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg atcacttgga
    16261 tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg ataaacgctt
    16321 gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc aacacaaaga
    16381 aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact gatccttttt
    16441 tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
//
LOCUS       DACENJ010000045        16285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.045, whole genome shotgun sequence.
ACCESSION   DACENJ010000045 DACENJ010000000
VERSION     DACENJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.045"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(31..894)
                     /locus_tag="IW748_001064"
     CDS             complement(31..894)
                     /locus_tag="IW748_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1103984.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1090..3189)
                     /locus_tag="IW748_001065"
     CDS             complement(1090..3189)
                     /locus_tag="IW748_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1103985.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3409..4518)
                     /gene="rpoD"
                     /locus_tag="IW748_001066"
     CDS             complement(3409..4518)
                     /gene="rpoD"
                     /locus_tag="IW748_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1103986.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4542..6383)
                     /locus_tag="IW748_001067"
     CDS             complement(4542..6383)
                     /locus_tag="IW748_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1103987.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6565..8958)
                     /locus_tag="IW748_001068"
     CDS             complement(6565..8958)
                     /locus_tag="IW748_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1103988.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9065..9925)
                     /locus_tag="IW748_001069"
     CDS             complement(9065..9925)
                     /locus_tag="IW748_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1103989.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(9984..10505)
                     /locus_tag="IW748_001070"
     CDS             complement(9984..10505)
                     /locus_tag="IW748_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1103990.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            10825..11544
                     /locus_tag="IW748_001071"
     CDS             10825..11544
                     /locus_tag="IW748_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1103991.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            11570..14161
                     /gene="mprF"
                     /locus_tag="IW748_001072"
     CDS             11570..14161
                     /gene="mprF"
                     /locus_tag="IW748_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1103992.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14349..15536)
                     /gene="tuf"
                     /locus_tag="IW748_001073"
     CDS             complement(14349..15536)
                     /gene="tuf"
                     /locus_tag="IW748_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1103993.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15904..16260
                     /locus_tag="IW748_001074"
     CDS             15904..16260
                     /locus_tag="IW748_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1103994.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4451 a         3378 c         2875 g         5581 t
ORIGIN      
        1 agctagttaa gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac
       61 accgttttct tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc
      121 tcctttgctg ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt
      181 gatttgctga tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat
      241 agcttcatat gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat
      301 cactcgacct tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat
      361 aaacccaaga tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc
      421 tgttcctatc acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa
      481 tattttttca tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat
      541 catcaagcga tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga
      601 tacgacaaag tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt
      661 agcagtaacg attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat
      721 ccgattttca tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg
      781 ttcaccttct gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt
      841 ttcattttca tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt
      901 ctccctctat agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat
      961 ctacttgaat agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt
     1021 gtcatactcc cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt
     1081 tgcctaaaat taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac
     1141 gatgtaaaat ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt
     1201 ggcatataga ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc
     1261 tccttcaacg atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg
     1321 tgcccacgtc catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat
     1381 tggctgattc gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc
     1441 ttgtgcgacc gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt
     1501 tgcttgttct tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt
     1561 tttctcttta tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga
     1621 tagatctgta tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt
     1681 tgctgaatcc actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg
     1741 caattcagca tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc
     1801 tgtgttatcc gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc
     1861 tagatgatca tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg
     1921 gatcttagat tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt
     1981 aagactggat aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat
     2041 aaattgatgg tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg
     2101 ttgctgtctt tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata
     2161 agcccagctt cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt
     2221 tgattttttc ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt
     2281 tttttcagta tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg
     2341 gttctcttct ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt
     2401 ttcagcatcg gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc
     2461 gagtatttct tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt
     2521 ttcagtaaat acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt
     2581 ttctttacct gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg
     2641 ctctactttg ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg
     2701 ctcttctttt gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc
     2761 ctcattatcg acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt
     2821 ttcttcttga ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc
     2881 atttttctct atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc
     2941 tttttcgaat aattccatta cagattctag aggtaaatct ttcaattccg accattcgat
     3001 tggatcgttt aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc
     3061 tgcatctgta tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg
     3121 atgaccgcag tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca
     3181 ttttttcatc acttagcgat cggctccttt actaggttta cgctaccatt taatctatca
     3241 tattctatac gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat
     3301 ttccttccta tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa
     3361 ataaaccaaa cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa
     3421 atcttttaat tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat
     3481 ctgacgaata cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt
     3541 ccggccatca tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt
     3601 atctagtaca tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact
     3661 tgttgcttcc tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg
     3721 cgtttcaagg gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg
     3781 aagatccatt tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa
     3841 ttgacgttgg atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat
     3901 cgtacgtgct tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata
     3961 ggttgaaaac ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat
     4021 gtttccttct tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat
     4081 acttactact aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc
     4141 ttcgattttc aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat
     4201 ttctttcaga tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc
     4261 ttctgttttt gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc
     4321 accattttca tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat
     4381 ttcatctgca ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac
     4441 ttgtcgtttg ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt
     4501 atctgttcca tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac
     4561 tgtttcgtta aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt
     4621 cgtctggctt cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg
     4681 ctgatcacat ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa
     4741 tattcgattt ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct
     4801 gcaaggttga attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca
     4861 tatgcatcat gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct
     4921 ttaaatgcac gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt
     4981 ttccgtgcag tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga
     5041 ttttgctgat ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc
     5101 tgaaactctg ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg
     5161 ctaagttcaa ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt
     5221 ttcccttgtt ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact
     5281 aattcttgga aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct
     5341 ggaacgacta cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga
     5401 ttggttgctt caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc
     5461 ttttccattt gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt
     5521 ttgataccag cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct
     5581 ccgtttttcc ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg
     5641 aacagttctg tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca
     5701 ggtgcaagcc agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac
     5761 atcactcttt gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca
     5821 gagcgttcta acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa
     5881 cgctcattag gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga
     5941 ctttgcaaat attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag
     6001 atttccgctg ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg
     6061 acagctatag tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact
     6121 tttaccactg cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt
     6181 ccgcctttac cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac
     6241 ggtgtacgtt cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac
     6301 tggacatatt gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc
     6361 tcttgaggaa tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt
     6421 aacagaagat atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga
     6481 ttttaaagga cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat
     6541 aacacatttc tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat
     6601 atcagccttc cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta
     6661 tcctacgctc tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc
     6721 tacatatatc cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata
     6781 acaagctttt acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt
     6841 agtttcagca tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac
     6901 tactacaagc aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt
     6961 gatttttctt ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa
     7021 atcaatcagc gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat
     7081 aatcgataaa ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac
     7141 attgttgacc actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc
     7201 caacaatacc agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct
     7261 gcaatctgct tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt
     7321 tctgccgctt tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc
     7381 aaaaaccgta tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag
     7441 atcgacatac gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa
     7501 aggattgtct ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt
     7561 ttcttgagcg ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa
     7621 tgctggtttt tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata
     7681 gccattcgct tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc
     7741 aggcgctcct acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc
     7801 agaagaaaat gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct
     7861 catagcccgc atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc
     7921 tgggatggct ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc
     7981 ttcagtaatg gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat
     8041 tgaatacatg tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa
     8101 tgcaatactt attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga
     8161 tgcaataaca gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat
     8221 gatccccaaa ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac
     8281 aagttcagaa cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt
     8341 atctgcacct actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga
     8401 aagcaattca gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc
     8461 atttgcttct gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc
     8521 gatttttacc aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc
     8581 ccggatcact agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc
     8641 atgtatttct tgataaattc caataacgat attgacgaca ataattgcta aatacgccat
     8701 gttggaatac gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa
     8761 taacgtcaat atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta
     8821 gtcattttgc tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat
     8881 tttctttttc tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg
     8941 ttccaagatt aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt
     9001 acaaaaaaga tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct
     9061 atctttactt atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta
     9121 taaaaagaac aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa
     9181 tgatcataat cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg
     9241 cctttgagaa aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa
     9301 ttccgatcgg cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat
     9361 acaatcccat atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac
     9421 caatcaatcc gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata
     9481 attgagcgcc gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca
     9541 catcagaagt atctcttcct tttttccctg cttctttcca taacgcagga acagttccag
     9601 cgatacatcc gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga
     9661 ctaaaaaaga aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca
     9721 aaacattttc tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca
     9781 tgccaaagac agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga
     9841 taaacattcc cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat
     9901 tttccatttt ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc
     9961 atgattgaac atttcctgct attttactta aagacttttt cataggtctc tattattttt
    10021 tcattcattt tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg
    10081 ccattgtaaa gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct
    10141 aaaagtgggc tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg
    10201 ccatgatttt ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc
    10261 cataaatcgc atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct
    10321 tgatcaatac tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag
    10381 ctctgcatga gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa
    10441 ttctcgtagt tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt
    10501 tccactcgtt ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc
    10561 gtcagaatga tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca
    10621 tcacctagtt attttgacat ctttttttat cttactattt ctttatcttc ttggctattt
    10681 cttccttgat ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc
    10741 ttacgaaaat gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt
    10801 atactttaca cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat
    10861 gggagcgaaa tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc
    10921 aatgacggca gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc
    10981 gtgtatctag tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat
    11041 tttcagttaa tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca
    11101 gattttcttg gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct
    11161 aaaaaagttc atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt
    11221 tctgccagag tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc
    11281 ccaagtcttc gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca
    11341 gaaaatgatt taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta
    11401 atcaagctaa aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt
    11461 gtgaaagaac aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt
    11521 cttcaatttc tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa
    11581 taccatacaa tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt
    11641 cattgtcatt attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt
    11701 aggagaaacg tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct
    11761 tatctctgtg ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa
    11821 gcagaatcct cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc
    11881 cggtttcggc gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga
    11941 gagcagaaac gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc
    12001 tatttacagt ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa
    12061 gcaatattgg atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac
    12121 tactttcaaa aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt
    12181 gctagtttca ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt
    12241 aatggaaatc cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat
    12301 aggaatcgtg tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg
    12361 gttgtccgct attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct
    12421 gttctactac atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc
    12481 cacttttgat cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa
    12541 aatcgtggtt gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc
    12601 gcaagctttt acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat
    12661 tatccaattt cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc
    12721 agcacgtgtg aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta
    12781 tgttttatta agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat
    12841 cattcttgct tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg
    12901 tacgatcgat ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata
    12961 caatctgccg gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc
    13021 tgaaaagatt tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct
    13081 atttgttcat ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa
    13141 ggccctgaag atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa
    13201 agataagcgg atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc
    13261 ttgcttcaat aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc
    13321 agaagcaatc gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta
    13381 cgaaacaagc gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat
    13441 gggggaagaa gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg
    13501 cactcgtgct gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc
    13561 accattttct actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc
    13621 acgaaaggaa aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc
    13681 tattgcagtt gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac
    13741 ttacacgaac aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc
    13801 ttctggcagt atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat
    13861 ccaatggttc aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt
    13921 tttacaggaa aggattgcat acttggttta cgagtttggt tctcattttt attctttcca
    13981 tggattaaga gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata
    14041 ttctagagat agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc
    14101 agttgagcag acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta
    14161 aaaactgcat accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg
    14221 aaagttcatg cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca
    14281 gaaatcctgt ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt
    14341 cgcgtttctt atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca
    14401 cgaattgaga atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc
    14461 gtcacgttgt caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta
    14521 acatcagttg tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt
    14581 ccaccttctt cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg
    14641 gaacctggtt tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac
    14701 aacacgccta cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca
    14761 cctgttacaa ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg
    14821 acacgaacag caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag
    14881 aagacgtctt ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata
    14941 tattcatcta ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc
    15001 aatgctttta atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg
    15061 ctcaataact cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa
    15121 tcaactttgt tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg
    15181 tgttcacgtg tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc
    15241 atttgtgctg cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata
    15301 tgcgcgtagt ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca
    15361 cgttcacgtt cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct
    15421 tttttgccta atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt
    15481 ccaatcgtac caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt
    15541 tacaaacctc ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata
    15601 gtcttttttt gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact
    15661 aagcttatta tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg
    15721 cgaaaaaagt accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt
    15781 ccttaacttt cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt
    15841 gccaacaact ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag
    15901 tacatgaaaa aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt
    15961 gcagcttatc attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc
    16021 gaaatgtcag ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa
    16081 gcggcttata ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat
    16141 ccattaaaga tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc
    16201 tgggaagaaa taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag
    16261 taaatggtct taaaatttta ttaga
//
LOCUS       DACENJ010000046        16253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.046, whole genome shotgun sequence.
ACCESSION   DACENJ010000046 DACENJ010000000
VERSION     DACENJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.046"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(117..1490)
                     /locus_tag="IW748_001075"
     CDS             complement(117..1490)
                     /locus_tag="IW748_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1103995.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(1490..1885)
                     /gene="rbsD"
                     /locus_tag="IW748_001076"
     CDS             complement(1490..1885)
                     /gene="rbsD"
                     /locus_tag="IW748_001076"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1103996.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(1913..2830)
                     /gene="rbsK"
                     /locus_tag="IW748_001077"
     CDS             complement(1913..2830)
                     /gene="rbsK"
                     /locus_tag="IW748_001077"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1103997.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(2938..3951)
                     /locus_tag="IW748_001078"
     CDS             complement(2938..3951)
                     /locus_tag="IW748_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1103998.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(4032..4631)
                     /locus_tag="IW748_001079"
     CDS             complement(4032..4631)
                     /locus_tag="IW748_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1103999.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4817..5518
                     /locus_tag="IW748_001080"
     CDS             4817..5518
                     /locus_tag="IW748_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104000.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            5528..8239
                     /locus_tag="IW748_001081"
     CDS             5528..8239
                     /locus_tag="IW748_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1104001.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLNKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            8690..10318
                     /gene="treC"
                     /locus_tag="IW748_001082"
     CDS             8690..10318
                     /gene="treC"
                     /locus_tag="IW748_001082"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1104002.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(10378..11559)
                     /locus_tag="IW748_001083"
     CDS             complement(10378..11559)
                     /locus_tag="IW748_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1104003.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            11811..13334
                     /locus_tag="IW748_001084"
     CDS             11811..13334
                     /locus_tag="IW748_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1104004.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(13403..14131)
                     /locus_tag="IW748_001085"
     CDS             complement(13403..14131)
                     /locus_tag="IW748_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1104005.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(14144..15424)
                     /locus_tag="IW748_001086"
     CDS             complement(14144..15424)
                     /locus_tag="IW748_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1104006.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(15565..16167)
                     /locus_tag="IW748_001087"
     CDS             complement(15565..16167)
                     /locus_tag="IW748_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1104007.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
BASE COUNT         5413 a         2978 c         3157 g         4705 t
ORIGIN      
        1 atgtaaatta taccaaagtt tggaaagtat cacaaaaatt tataaaaata atgatcctaa
       61 aaagaaaaag actgaaatat gtgataaaca tctcatactt cagtcttttt cattaactat
      121 tcataagaca ttaggtcttc atcaaacccg ctaacgagag tagtgatcat tgctacgact
      181 agactaacag ccagtcctgc taagtaccag aaaaaagtac cggaaatgaa tgcaggcaat
      241 gtggtcagac taccaaatac aaaagcgatc gctttcactt gggcaaatcc cattacagct
      301 ccaccagcag ctgcaccaat gcaagcagca acaaatggtt ttttcaatgg gatcgcaatt
      361 ccgtaaacag ttggttcggt cactcccgca agtaaaatcg aagcaaaagc agaaccggag
      421 atcgaacggg tttttgaacg tttacttcgc agaaatacac ctaaagctgc accagccatt
      481 ccaaaattgg ctgctcctgt caacggaacg attcggtcga acccataagc agagatatta
      541 ctgatcatga cagggtttac agcccattgg ataccaaata ttaccaaaat attccaacca
      601 ccagcgatga ttgcgccact gatcggtccg ttaatatcca tcatccaagt aattgcggca
      661 gctaaacctt cgccaaggta aacacccgca ggcccgaata ccattaacgt caaaggaacc
      721 gtgataacta atgaaagtaa aggtacaaat actaattgca tattttcgcg aatatatttt
      781 ttgaggaatt tttcaacaaa agaataaaag aaaataccta aaatcgccgg catgacagta
      841 gacgtatact gcatcagtac tacaggaatc cccagaaaat cagtagctgc tttgccactg
      901 tctgaaagta aagaggtcag attcggttca atcagtgctg cagcaatgac agccgcaata
      961 tatccattca ctttaaacgt tttggcagca gaaaaagcaa gaaagattgg cagaaaataa
     1021 aagacggcat tcgacgcagc agacaaaata tgataagttc cgcttgtttc actaagccag
     1081 ccaatcgtag tcgctaaaac aaccagacct tttaatatac cagaaccggc aaacagcgga
     1141 atgatcggcg taaagatttc cgatatcaaa ccaaatagtt tttcgatcgg atttagcttt
     1201 tctttcggag ctgaatgatc gatttgacgc gtcaattcct tgtaagcagg agccaatccc
     1261 tgatccatga ttatttctgc atatctatcg agtaaaatga aatacttaat ggaagggatt
     1321 tgctgtttta atttttctgc attggctact ggctgttttt tcaatttgat acgaagccga
     1381 gtcgcacatg gaaggacaga atcaatattg tcttttcctc ccaactgttc aatcagcata
     1441 tgaatctgct cggacagtac catctgttca ggattcattg tcgtttccat tagaagacca
     1501 ctccactcac taatataata ttggcgtaag gtgtcatttc gcctgtacga ataaatgcat
     1561 gacagttatt caactctttt ttcatttctt catgtgggat aaagctaatc ggtgtatctg
     1621 gtaacaataa tttgattttt tccaatattt ccgggttcat tgtctttatt tcttccgcca
     1681 attcaatcga ttgtacctca agttcttcta aaacgttttc taaaacagat aaaaaacttg
     1741 gaagaccgtt tgagacagcc aaatctattt tttcagtacc aaaggggaca ggcatgcctg
     1801 catccccgat acttaattta tcaaagtgcc ccatttgtgc gattactcgt gaaatatctg
     1861 aattgattac ttttcctttt ttcatgtttg tttcttcctc tcattgttaa ttttagactg
     1921 ctgattgttc taaagcttcg cggtaaggga tagaaggttg cgcaccataa gactgcacag
     1981 caacagaaga ggctagattt ccataaagga tcgcttcttt caagttacta aggtctggct
     2041 gaagtgtgct gcttaatgcg ccaataaatg tatcacctgc ggctgttgtg tcaacggcat
     2101 ccactttaaa agcagggacg ataccgtgtt ctttttctgt atgatagaaa gcgccggcac
     2161 tgcctagtgt aatgattact gttccaatac ctaattcatg gagtttttcg gctgctgcga
     2221 ccaaactgtt atgatctgtc actctgattc ctgtaatgat ttctgtttct gtttcatttg
     2281 gtataatgat atctgtcttt tcaagtagtt cagtaggaat cgtttcacga gctggtgcag
     2341 gattcaggat cgttgttttt cctgcgtctt ttgcaataga aaaagctagg atcgtcgcat
     2401 ctaagctact ttcaaactga gcaataataa agtcactgtt tgtaatcagt tgcttgtttt
     2461 ttaggacttc ttgttcatgg aaagcattat ttgcccctgc atggatcaag atactatttt
     2521 ctcctgctgc gtctaccata atcattgctt gtccggttgt ggcgccttgg atttcagcta
     2581 tcccagatgt atcgatgttt tcttttgtca gtagatctaa taactgttgt ccttcgctgt
     2641 cagccccaac gcctccaata aaagatgtcc gtgcgcctga acgtttggct gccactgctt
     2701 ggttggcacc tttacctccg ccagcgtgaa aaatctcatg cgcatgcatc gtttcgcctg
     2761 gcttgggcat gttggttaaa cgaagggtag tgtccatatt aatacttcca attactgtaa
     2821 ccgtgttcat tttacaaacc tccaactatc tcatttattt tgatgggaaa attatttttt
     2881 aaaacgtttt agtaaaacgt tttaatagtt cgataaaaat atagcacacc ttgaaagcta
     2941 tttcaagggt gcagttgaaa atcttttttc taatcgtaca ggtaatcttt tttcttccca
     3001 ttctttttct gggaattgga tccgatctaa caataacttg gcagatgttt gtcccaattc
     3061 gaagattggt tgtgcgatcg ttgtcagttt cggcttgata tactcgcaca tatctatatt
     3121 atcatagcca ataatgctgt agtcttcagg gatgcttttt ccagcttcct ctaatccgcg
     3181 atataatcca aaagctaatt catcatttaa agcaaacaca ccagttgcag cagaatcaag
     3241 aagttctgct gtgatctggt agccaccttg ttttgagaac tgtgtaggta agaggatcag
     3301 ttggtcgtga gggatttggt aaacatcaag agcagattta aacccctcaa tccgagcgtg
     3361 gacattttcc ggtggatttt caggatagac aagggcgatc gtttgatgtc ctagactaag
     3421 caagtgcatg cccgctaggt aaccaccgcg aaaatcgtct gttcgtacgg catcactaaa
     3481 tccttcagat tttttctgat ccaaaacaat gaagggccgg ccttgttttt tcaaattttc
     3541 gttgattgca tctgtagaga cagcagaagt cgcgataatg aatccatcca cacctcgacg
     3601 tgtgagctcg gctagatatt cgatttcttt ttgatgatca gaatcagcat tacataatat
     3661 cgtgacgaaa ttctgcttat acaagatatc ctcaattcct cgcatcagtg tactgaagaa
     3721 tggattcgtg atatcaggaa cgagtacacc aattgttttc gtttccttgg taatcatttg
     3781 tctagcaaaa tagtcaggct gatagcctaa ttcttctttg gcagctaaaa ctttctgaac
     3841 agttttcgga ctgaatttct ccgtatttcc atttaatatc aaggagacag ttgtgattga
     3901 aacgccggaa agtttagcga cttctttgat tgtgatcttt tttttcacca tgttcatact
     3961 cctatctaaa tcctgtggtt attttcccat gaaatcaaaa agaagtaaat gaaaatcacg
     4021 tgtgccagta cttatttgct ttgttggata gcttcgatta tttgtttcgt tgagcgtact
     4081 cgtgcagttt tcggaaatat cttttttatg aacaattgat gggattcttt gtcacgatca
     4141 ctgcaagcat cttcagcaag cacgatatgg tacccgtgtt gaaaggcatc taatgcagta
     4201 gcatatacac cgtttgttgt cgctacaccg cttaaaatga tagtttcgat tccacgccgg
     4261 cgaagttgaa gatcaagaga agtaccaaaa aaagcacttg gattgtattt tgtgatagtc
     4321 aacatattat ctgtatcttt caaagaatcc ttcaaaagta aatcagtgaa ttctggaggt
     4381 acagaagcag tcattccacc catatcagtt tgttgactca aataagggaa agtggagatg
     4441 tctacgttca ctaaaacttt caaggcagcg gtacgtttca atgttcttac taacaaatca
     4501 ttgtttgtca gaatagatgc tgcagtgtgt ggagagagtg taccgttatt tacgatctct
     4561 ttttgcatgt cgatcgcgac aaaagcagtt tttgataaat caaaaatcat attttccttt
     4621 aaatcattca tgatgttcct tctttcaaat aacaatgttt tttatgtaag taaatcataa
     4681 atcgtttatt tgtcaactaa attaaaggat tcctttcttt agtctagaga aattcagtgg
     4741 taaaataaat aagtgtgaga aaacgcattc gatgcataga tatagcgaga aatatcgtga
     4801 aaatggagga atcagagtga agtatgtcga agtaaaaaat gaatacaaac gatacaaaat
     4861 gggagaaacg accattacag caaatgacga tatttctttt tcaattgaaa aaggcgaatt
     4921 agtgattata cttggtccaa gtggagcagg aaagtcaacg attcttaata ttttaggtgg
     4981 gatggataca cctgatgaag gtcagatcat tattgatgac acagatattg cgcaattttc
     5041 agataaacag cttactgctt accgacgaac agatgtaggg ttcgttttcc aattctacaa
     5101 tctcgttcct aatctaacag caaaagaaaa tgtggaattg gcaacggaag tttctcctga
     5161 tgcgcttgat cctgtagagg tattgaggca agtgggccta gctcatcgat tgaataactt
     5221 tccgtcccaa ttatcaggag gcgaacagca gagggtttca attgctcgtg cactagctaa
     5281 aaatcccaaa cttcttttat gtgatgaacc gacaggcgcg ctggattttg aaacgggcaa
     5341 gcaagtattg aaattattgc aaaacgctag cagacaacac ggcaataccg ttttgatcat
     5401 tacacataat tctgctttag cgcccatcgc tgatagagtc attcacatta atgatgctaa
     5461 agtaaggtct gttgaattaa atgatcatcc ttcatccatt gatgagattg tatggtaggg
     5521 ggattttatg aaaacaagaa catatcttaa agcaggtttt cgcgaaattc ggcaatctaa
     5581 aggacgattc attgcaatca tccttattgt catgctagga acacttttat ttgtaggtgt
     5641 caaaactgct ggacctgtca tgcaaaaaac gatggatcac tatgttggta cagcaggact
     5701 ttctgatttg caaatcgttt ctactggtgg cttgacacaa aaagatattg ctcaagcaga
     5761 aaagatccca gatgcaaaag tagaaacagg aaaacagatc tattattcca atactggcaa
     5821 aaacgaagtg attcaaatat tttcttataa caaaaataac aagcagaaca agctacaatt
     5881 aactgatgga aaactcccgg aaaaaccaaa ccagcttgta ttggatgaaa aagcggaaga
     5941 tgaagggtat aaaatcggtg atatttatca gattgattca gatgaattga aagaaaaaga
     6001 gtataccatc acgggctttg tccgttctcc attgttcatc aacaatttgg agagaggata
     6061 cgcgaacgtc ggaaatggta gcgtcgatta ttttgtctat ttacctgaat ccgtttttaa
     6121 aacagatatt tattctgtct tatatcttga ttttacaaac gtaaagaatt tgaatacgta
     6181 cagcaaagct tacaaagata aaatggagca aaatcaagaa aaggcagaaa agtatttaca
     6241 agatcgaccc gaagaacgct tagaagagct gaaaaaatcg gccaacgaag aattagaacc
     6301 ggcacagcaa aaaatagcgg atggcaaagc acaaacaacg caagcaagaa cacggttaga
     6361 agatgctaaa aaacagttag cgcaacagtc agcaactatc cagcagcttc ctgaagcgca
     6421 acgaacaatc gctgttcaaa cattatctca acaagaagct caacttgctg aacaagaaag
     6481 gcaattagtt gagaaagaac gtgaactggc ctctgcagag gaagaattga atcgaaatgc
     6541 agaaaaaatt gaaaatttaa ataaaccatc atacctatac aatgaacgtt cagaaaatgt
     6601 cggttttcaa gaatttggtg atctgtctga tcgtatcgct gcgattgcca atgtttttcc
     6661 tgttttcttt ttctttattg ccgcattgat cacctttacg acaatgacac gtatggtaga
     6721 agaaaaccgc agggaaatcg ggacattaaa ggcgttaggt tatacgaaag ttgaaatcgc
     6781 aaaaaaatat gcaatctatg caagtctagc ctcagggata ggtattgttt taggaacgat
     6841 attaggaacg aatcttttgc caaggatcat ttttgagctg tctaacgagc ggtatgatat
     6901 tgggagtgcg cttatttttt atgactggcc gccgatttta caagcagctg ccgcattctt
     6961 cattgccgca tttggcgctg caatgatcgt tttattcaaa gatttaagag aacggcctgc
     7021 tgcattgctt caaccgaaag caccgaagcc agggaaacgc atttttcttg aatacatcac
     7081 tccgctgtgg tcacgcctta gctttaatca aaaaatcagt taccgaaatc tcttccgata
     7141 taagtcaaga atgcttatgg cgattatcgg aattgctgga tgtgccgggc tgatggttgc
     7201 aggcgtggga ttgaaagatt ctctaagttc tgtttcagcc aaacaatttg gaacgatcat
     7261 tgactatcaa gcaatcgtta cattggattc atccacggaa gatagtcgtc aaaaagcagc
     7321 ggatatgctg cagaagcaag aaaaagtaaa tgagatccta gctattcata ctcaaaccgt
     7381 agaattgaga aaaaaaggac atgcttcgca aagtctgaca gtgatcgtac caagtaaaac
     7441 agctgatttg gatcgtttta tccatttaac tgatcttgaa gagaacccac gctccttgcc
     7501 taaaacagga atcgctatta cgcaaaaagt agcagaacta tttgacttat ctacaggaga
     7561 tcatatttcg ctttatgatg aagatcaaaa agaatggaaa gtcgaagtaa aagaaatcgt
     7621 gaaaaactat ctaggtagct ttatttatat gtcccctgaa tattatgctt cagttactga
     7681 tcaatcgatg atggataatg catttttgct gcgtacagat aagatgacaa caaaagaaga
     7741 agaaatgctt tccgagaaac tgcttgccac agacgctgtg acaaatacgt ctttcatgtc
     7801 caaacaaatc gaaacgcaag aagaatcaat ggcgaactta gactcggttg tcttaatctt
     7861 tgtcgtatta tcaggactgc tggcttttat cgtcttgtat aatctaacta atatcaatat
     7921 ttccgagcgt gtacgcgaat tatctacaat aaaagtcctt ggattttttg ataaagaagt
     7981 cacgatgtac attgttcgag aaaatatcat ctttactctt ttaggaatca tcggaggatt
     8041 tggtgtagga tatatattga ctgacttcat cttgcaacaa gcgtctatgg agaatgtcat
     8101 ctttccatta gtgatcacat ggtgggctta tgtcttatca gcaggattaa ctatcgtatt
     8161 tacggttatt gtgatgattg ttacccattt caaattaaag catatcgata tgattgatgc
     8221 gttgaaatcg aatgaataaa aacagaggtt gtgaaatcag cggtttgacc tgagtattag
     8281 agaaacaaac ggcaaaatag tttttcatat tttgccgttt tttgagctaa taccgaagga
     8341 cagctgattg aacaccatga atcagaggtt gtaaaaaagc cgtcctgcat cgagcaaaaa
     8401 gaatactcaa ataaaattag ctcctcatgt tttacatttg agaagactga ttgccgtaga
     8461 taaaagcttt tgaacaccat ttatagaaga aacaagaata ttttgctatt ttaaacaaga
     8521 ggatagacaa atacatgtct gatctcttgt tttttattgg gcaacagacc tcttagaatg
     8581 tataaagata aattttacaa acgctttcgg ttcagttata ataataaatg ataacttgta
     8641 tatataagtt gtggtaggta ataaatcagt tgaatggagg taaatatcca tgacatttaa
     8701 agacaaagtc atttaccaaa tctatcccaa atcttacaaa gacagtaatg gtgatggaat
     8761 tggagattta aaaggaatca aagaaaaact cccgtatctg aatgatctag gcatcgacat
     8821 gatttggcta aaccctattt atccaagccc tcaaaaagac aacggttatg atatctctga
     8881 ttacacagcc atcgatccta tctttggtac gatggaggaa ttcgaacagc taatagccga
     8941 agcaaagaaa taccggatag aaatcatgct cgatatggtg ctgaatcacg tttcgacaga
     9001 acatgaatgg ttcaaaaaag cactagcagg ggaccagcat tatcaggact tcttcatttt
     9061 aagagaagag ccaacggatt gggtatcaaa atttggtgga agtgcttggt cgccatttgg
     9121 agatacgggg aaatattatt tgcacttata tgatcggaca caagctgact tgaactggcg
     9181 gaaccaagca gtaagagagg aactattcaa agttgttcgt ttttggatgg aaaaaggggt
     9241 aaaaggattc cgtttcgatg ttatcaatgt tatcggtaaa gacgaacaat tgaaaaataa
     9301 taaagaaaat gatggcaaac ctgaatatac ggacaaacca atcacccata cgtatctaaa
     9361 agaattgaac caagcaacgt ttgggacgga ttccactatc atcacagtcg gtgaaatgag
     9421 ctctacgacg atcgaaaatt gtatcttgta tacagaacct agtagagaag aattatcaat
     9481 ggtctttaat ttccatcatt tgaaagtcga ttatgaaaat gggaataaat ggtctactcc
     9541 tccatttgat tttgcagaat taaagcgact atttcataca tggggagaaa agatgagtga
     9601 gggtaacggc tggaatgctc tattttttaa taatcatgat cagccacgtg cgctcaatcg
     9661 atttgtagct atcgatcagt atcgaaaaca aggagcagag atgctagcaa ccatgatcca
     9721 tttgaaccgt gggacgccct atatttatat gggagaagaa atcggcatga tcgacccaga
     9781 ctattcatcg atttccgact atattgacgt agaaagtttg aatgcttata agttactcct
     9841 tgaacaagga ttcacagaaa aagaagcgtt tcgtcggatc aaagcaaaat ctcgcgataa
     9901 ctctcgaaca cccatgcaat ggtcagacac agaacaagca ggatttacta cagggaaacc
     9961 ttggttaaaa gttggaggca agctagaaga gattaatgta gaaaaagaac tatcttctga
    10021 agattctatt ttgtcctatt acaagaaatt gattcggctg agaaaaacat atccaatcgt
    10081 tgcacaagga gattatcatg cttatgaagc aaatcatccg gaagtttatg gctttcttcg
    10141 ccaatttgaa ggacagcagc tgcttgtatt aacaaatttc tatgataaag atacaaggat
    10201 cgccatccca gaaggattca cagaagccaa tattcttatc gataattatt ccgatactgt
    10261 tcttcatcaa agtctggagc taaaaccata ccaggcaatt gctttgctga aaaattaaaa
    10321 caaaaacaca aaacgtcgtt cctacgagct attctcgtaa gatcgacgtt ttgtgtttta
    10381 tttaaaataa tcttcttcag aaaatgaaga gttttcatac tctcctttat cgccataaaa
    10441 tttattgtag cgttgtttga atttacggaa aataaaagta acgagtaatt tgactaacgt
    10501 atagatagga ataccaaaaa taacgcccac aattcccatt agatcaccca tgacaagtaa
    10561 aacgattaag atcgtaatcg gatggatctg taaacgatca cccattactc gaggaatcac
    10621 caagtcaccg tggagaagtt gtacggcaaa ccagacaatg acaaatttga cagccataaa
    10681 agtagagtct tggaatgcaa tgatcaaacc tggaaagaag gcgataaacg gcccaatata
    10741 tggaatgata cataacaacc cagcagaaat agctaaaaca ctagcaaatt ctaatccgat
    10801 cagtaagaaa caaacccagt agactgctcc aaggataaat gaagcaatga tttgcccctt
    10861 tagaaatgaa cccaattgtt gattagcaat ctttgtcaaa tttttaaaat ctgtacgaaa
    10921 tgctggtggg acaatcgcta aaactgactg ataaaatttt tgaggatctc tcaaaaggaa
    10981 aaaagcgaca attggaccag taaataaagt aataaaaata gctgtaacag ttgaaaaaat
    11041 gttgctgatc cgttgtgcgc cactttccca ataatttcca ataaatccag ctatatcaga
    11101 cgtcacgtcg tcaatagaag cgaaaaattg attaaatgaa tcggcaaaag gtgttttctc
    11161 caaaaattgt cggagactct cttgaaaatc ttgaaacaaa tctggcaaac tactgatcaa
    11221 tgtctttgcc tgttcttcaa gaaaaggaat aagccatatg cttccaagcc ccaataaaaa
    11281 taaaataatc acataaacga taatgactga caagttcctc gaaaggtgct tgctcaaacg
    11341 tttgatgact ggttccaaaa gataatacaa gatcagtgca aacaataatg ggggcaagat
    11401 agtagcgata atgattccga caggcttgaa tataaacgag atctgatgga aaaaataaat
    11461 gatcagcgca agtaagataa ctattcctaa aacatagtaa gaggtttttc ctcctaaaaa
    11521 gcggatcaaa cgattcgttg tatctgtttc ttttttcact ctttttcctc ctttatctat
    11581 aacttcatta tagacaaaag cgggtataaa aggaaaatga tagaggttga ttgcaaaaat
    11641 atcgtcaatc attcatataa actttctcat caattaaaat aagaaaacgc ttgacagttc
    11701 ttttaaccat atgataaatt aaatcctgtt gctaaaacgt aactattacg gtttgcgaaa
    11761 agcgaatcgg aacattcaaa tgaaagaaga tgagaagagg agtgtcataa atgaaacgat
    11821 tgataggtat attggccatg ctggttttag caggtatgtt aactgcttgc ggggcaagtg
    11881 ggaaagccga ggacagtaaa gaaaaattga gcgtgatgac gaccttttat cccatgtatg
    11941 attttacaaa agcaatcgtc ggggatgaag gagaagtaga gttattgatt ccagcaggga
    12001 ctgattccca cgattatgaa ccttctgcta aagacatggc aaagatccaa gatacagata
    12061 tctttgttta taatgatgaa aacatggaaa catgggtccc cgcaatccaa aaaacattac
    12121 aagaaggcaa tgtccatacg ataaaagcga cagaaggtat gcttttactt ccgggaagtg
    12181 aagaaggaca tgatcatgac catgaacacg gggaagaagg ccatacacat gagctagacc
    12241 cacatgtatg gctagctcca agtttagcaa tcaagcaggt agcaaacata cgtgatcagc
    12301 tgattgaagc ttacccagaa aaacaggaag tatggacaaa aaatgcagca gcctataccg
    12361 agaaacttca agcactccat cagctttatc aagaaacctt taagcaagca aaacaaagat
    12421 cttttgtcac gcaacatgct gcattcaact atctagcgtt agaatatgga ttgaatcaag
    12481 tatcgattgc tggtctttct tctagtgaag aaccgtctgc ggcgagaatt gctgaactga
    12541 aacactttgt taaagaacat ggaatcaact atatttattt tgaagaaaac gccaaagata
    12601 gtattgccag aacgttagca aacgaagcag gtgtatcttt agaagtattg aatcctttgg
    12661 aaggattgac gaatgaacaa atagaaaatg gagaaaacta tctttctatc atggaagcaa
    12721 acctcgaagc gttgaaaaaa acgacagaga cagaaaaccc gctagaagat actttgaccc
    12781 ccaaaaaaga aaagagcgtc tacaacggtt attttgaaga cgaggatgta caagatcgct
    12841 ctctttcaga ttgggcagga acttggcaaa cagtagatac gtatgtagaa gatgggactt
    12901 ttgatcctgt ttttgaatat aaagaaaaat tgaatcagga taagacagca caagaatata
    12961 aagaatatta tacgaagggc tatcaaacag atattgcttc aatcaagatc agtgagaaag
    13021 agatggcatt tacgtttaag aatggaacaa cgaaacgttc aaattatcgt tatgtaggga
    13081 aagaaatctt gacctacgag gcaggaaacc gtggcgtaag gtatctattc gaagcagaag
    13141 ataaggaaag tggcgctttt agatatattc aattcagtga tcacacgatt gcaccggctg
    13201 actccgaaca ttttcattta tatgcaggaa atgaaagtca agaagtattg ctgaaagaaa
    13261 tggagaattg gccaacattt tatccagata caatggatgg acgtgaaatt gcacaggaaa
    13321 tgcttttgca ctgataggca aaaaagaacc gaagggtttg gatcggctgg tcatttagtc
    13381 ggtccaaact attcggttct ttttatgaaa tcaatttctt taatccacgg atagaggctg
    13441 ggatggtctc gccgtttgcc aacgtgactt ctctggtttt tttattaatc gaccgcacat
    13501 tttgtgtatt gataacatag gatttatgac aacggataaa attccgatgc attttctcga
    13561 tttctttgat cttctcatag aattgtaatt gggcatgagt caaatggact tccagtttat
    13621 gtggcgctcc tagcgaagta gaaaaatatt ggatgtcttt gatcggtaaa aatttgatct
    13681 catcattaaa ctctattttg aagatatctt tttggatttg ggacatttga aaatcttcga
    13741 aaattttgct caggatatgc tcgacatttt cttttaaatc ataaagatct tctttgacga
    13801 tataatcagt cggttcgatg ttgcttttta agattttcat cgacatttcc gtatgggaag
    13861 tcacatagat tatttttcct aaaggatcta attcacggat caatttaccg atcgctacac
    13921 catccatttc actgtcttct agttcgatat ctaaaaagta aataccaaaa gaagaagttc
    13981 tgatcttggc tgcattgaga agttcttccg gatcagaggt agaaatatgg acataactgt
    14041 ctaagttttc aatcatgatt ctatttttga tcaccgttga taaaacttct aattgttttg
    14101 gatcgtcttc acaaatataa atgggaatca tatcattact cctttatttt atttgtatat
    14161 aaatcgtttg agaaaaacgc tggttttcaa ttgatgtttc aaggaaaaga taaggattgg
    14221 tctttgtcaa ttcatccaag ataaacaagc caaggccttc gttttttgga tttgtttttg
    14281 ttgagaaccc tttttgtttc aactggttta acggtaatgt atttgttgtt gtattgatca
    14341 cacgaatcat cacggaatga ttcttcttga gtatggtcaa ttcgattttt ttcttctttg
    14401 cagcactagc tccttcaatc gcattgtcca agataatacc aagcatacgt acaaggttta
    14461 ccgtatgttt cgtatccagt tcgattggct caggaaaatg aagctggacg atcaaccctt
    14521 tttcctgtgc catcatgagt tttaatgaca gtaaactacg gatctcagga actttcatat
    14581 tatccaacat acttagattg gccggaaaaa ggttcatcat ctggcgtgta ggtaatatcg
    14641 tttgatcata ataagaccgt aatccttcta agtcgccttc tgaaatatag ctgtgaagag
    14701 aaaaaagcag attttggtaa tcatgtttga attctctcaa gtcttgatag tttttttcta
    14761 gatctttgat ataatcctgt tgcatcctga cttgttgctc taataactga tcagctaatt
    14821 tcttattaga gattttccaa taataaacca agatgatcag gccaacacac agatatagaa
    14881 tgaaaaaact cgtattcaaa gctaagacat caggtgtttc accagaaaaa cgggtataaa
    14941 aaataatgat atagtatgta aataatatca atgtcccgat cgtaccgatc atacggcggt
    15001 ccacagtgtg aaaccaagtc tctaacgatt tttttaaaca aaaagctaat aaagcagata
    15061 aaaaagcgga taagacaatg tgaaaatact gcaatgaatc tcctggttca ataaccgaag
    15121 atttcaaaac aaaaaaatcg ccgatgcttg ttaagtgatc tcctaaaagc gacgtcaata
    15181 acccaaaagg aagcgcgtaa accaattgtt gacgcgaagt cgaaaagaag aaaaaagtaa
    15241 gcatcagcat tcctatagca attagagcag taaaaatccc taaaatcgaa tagacccatc
    15301 caatcaaaat acaagaaatc agaatgactc ccgcaaacag atatttgtat cggatgtgat
    15361 ggtcaatcaa taagatacaa attgtgtaaa tcaaatattg caacgctatg gaccataaaa
    15421 acataaagta cagctcccat tgatttttac caaattatag cacaattggc gtattatttt
    15481 tttaattttt cagggatttt gaattcagaa ctcataccaa agcagcagcc agatacaaaa
    15541 aagtcagata agttagttaa ttgtttaatg attgtttttt tcatgaaaga ttcctccaaa
    15601 attcatcaag tagtttgtaa taggaagcaa cgtagctatt tctaaaacaa tcccaagtag
    15661 caaataggta gaaaagtaag aagctgagaa aaataacgta atgagagaaa aaattagaaa
    15721 catgctgatc gccttcttta ataatggggg acgtttctcg ggttcaatgg gattgattgc
    15781 tgtacctctt gatccatagc gaagcagtaa caaaaagcag ctgattaaca aaaagaccat
    15841 cattgggaag gagatagtca attgcagatg tttcatcacc aagggaacgc cccataaata
    15901 aagtagtgtc cacagcagac aaacgaattc agagtcggaa tgcaatccat aagcatatct
    15961 gcggatgcag atataagaaa gaaaggtgat tcctgtttcc gcccatgagc ctgtcaccag
    16021 agaaaagact agaacgaccg ctagttttcc aagattattc aataaaactt ctaagacaaa
    16081 ttttacttgg acatacacca tatcttcttc cgatttgtct ccgctgaata aggcttctaa
    16141 taatttgttc acaactttat ctttcatctc atacgtacct ccaccacaaa ttttatgcta
    16201 aaaaaaataa aaaacgtctt ttgtttcata aaaagtggtt ttgacatcaa aac
//
LOCUS       DACENJ010000047        16085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.047, whole genome shotgun sequence.
ACCESSION   DACENJ010000047 DACENJ010000000
VERSION     DACENJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.047"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(182..601)
                     /locus_tag="IW748_001088"
     CDS             complement(182..601)
                     /locus_tag="IW748_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1104008.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(603..1187)
                     /locus_tag="IW748_001089"
     CDS             complement(603..1187)
                     /locus_tag="IW748_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1104009.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1234..2709)
                     /locus_tag="IW748_001090"
     CDS             complement(1234..2709)
                     /locus_tag="IW748_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1104010.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            2890..3201
                     /locus_tag="IW748_001091"
     CDS             2890..3201
                     /locus_tag="IW748_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104011.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3316..5652)
                     /locus_tag="IW748_001092"
     CDS             complement(3316..5652)
                     /locus_tag="IW748_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1104012.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            5794..6462
                     /locus_tag="IW748_001093"
     CDS             5794..6462
                     /locus_tag="IW748_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1104013.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6465..7508
                     /locus_tag="IW748_001094"
     CDS             6465..7508
                     /locus_tag="IW748_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1104014.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(7745..8626)
                     /locus_tag="IW748_001095"
     CDS             complement(7745..8626)
                     /locus_tag="IW748_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1104015.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            8785..9513
                     /locus_tag="IW748_001096"
     CDS             8785..9513
                     /locus_tag="IW748_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1104016.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            9669..9986
                     /locus_tag="IW748_001097"
     CDS             9669..9986
                     /locus_tag="IW748_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1104017.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            9979..10587
                     /locus_tag="IW748_001098"
     CDS             9979..10587
                     /locus_tag="IW748_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1104018.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLVWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            10603..11433
                     /locus_tag="IW748_001099"
     CDS             10603..11433
                     /locus_tag="IW748_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1104019.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            11571..12059
                     /locus_tag="IW748_001100"
     CDS             11571..12059
                     /locus_tag="IW748_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1104020.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12110..12982
                     /locus_tag="IW748_001101"
     CDS             12110..12982
                     /locus_tag="IW748_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1104021.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13158..13520
                     /locus_tag="IW748_001102"
     CDS             13158..13520
                     /locus_tag="IW748_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1104022.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13529..14281
                     /locus_tag="IW748_001103"
     CDS             13529..14281
                     /locus_tag="IW748_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104023.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14295..>16085
                     /locus_tag="IW748_001104"
     CDS             14295..>16085
                     /locus_tag="IW748_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1104024.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLK"
BASE COUNT         4996 a         2802 c         3296 g         4991 t
ORIGIN      
        1 ccatgtttac aaaatttttc ttactactcg aaacgaagcg gctttatcac aacctcttga
       61 taaacggcgt tcaatcagct gtccctcggt attagctcaa aaacggcaaa atatgagaag
      121 ctatttttcc atttttttct ctcatactca ggtcataacg ctgatttcac aacctcttct
      181 ttcataatgg tttttcacca tctttataat aaactggttc tttgatatgg aaatctattt
      241 tttccaacca atctaaaatc ttgtaaccta actcttctga catcggaaaa gaggcgttga
      301 tttgtgcatt ggagatatgg tcaacatgga taccggaagt gatcgtacaa cttcccggaa
      361 cggcagtttt gatgatcaca gctatttttt cggctaagac atcgtcttta tggaaccgac
      421 catcgtggct agggaaacgt acagtttgca tctctgtatc acgagaaaat gtagtgactg
      481 ttccgatatg tggtgtatct ccgcctgtta aagtgatcaa taagtcttta ccgattactt
      541 taacagcagc ttggatcgtg aaacctagtt tggttacttc aactatcgtt tctttttcca
      601 tattaatcac cctgccaacg ttttgaatgc tcatttacaa tttcaaattg atccaaaagt
      661 ttcattgtat ggtgattaac aagatcttgg atcgtttgag gctgattata aaaagcagga
      721 attggtggta tgatttgcac acctagtcgt gctaatttag tcaagttttc caagtgaatg
      781 gcatttaatg gcgtttcacg aggaacaatg actaactttc gttgctcctt cattgttaca
      841 tcagcagcac gtgcaatcag gttgtcgcct aaaccaacag agatacctgc gactgttttc
      901 atgcttgccg gtacgatgat cattccatcg gtcaaaaatg atccgctggc gacagcagca
      961 cctaaatctt tgtttgaata atggtaatca aacaggcttt cgaattcagt tagtgataag
     1021 tcggattcca gctttaagtt ctgttttgcc catggactga tgattccgtg tgtttctatc
     1081 atcggatatt cttttagttt cttcactagg ttgatggcat aaatggtgcc tgaagcgcca
     1141 cttacaccaa tcacgatttt tttcttccgt tgtgccaatt gttccatttt ccgcactcct
     1201 ttttatgatg tcaaaacagt tgcagatcat tcattatttc aaatattttt cccaatcttc
     1261 tacttcttgg aattttgcac gtacaaaatg ttctttcata tcgaaaggaa ctgtaccgtc
     1321 aaagatagtt tttgcactta tcccacggaa acgaatagag ccaggattat attctggtaa
     1381 ttctgatggg tccaatggat ggttcctcat tcctgggatc acgatgatat ctttatctcc
     1441 ttggaaacga gtgttgaaag tccaaactac atcgttcatg tcgaaaatat caacatcttc
     1501 atcgactaaa atgactgtct tcaattcttt gaaggctgat aaagcaagaa tagctgcttg
     1561 acgctgaata ccttcatccg cttcgttttc tttacggatt tgcatgatgg tcatcaattt
     1621 accaccacct gcaggaggat tgtagacatt tactactttt cctggaatag ctttatcgac
     1681 caaggcaaga atacttgctt ctgtaggaat accggccata gatacgtgtt cttcactggg
     1741 accaatcgtt gtttgcatga ttggattgtc ttttcggtga gtaactgctt tgactttcac
     1801 tacttgtaca gctggattag catccccagt atagccagga aattctggca ttgcttttcc
     1861 tgagttcgtg ttgatatctt cttgcattgt ttcatttggc atgatttctg cttcaatgat
     1921 gaattcagca cgagcaatac ccacttcatc gactgctata ccatcaacga tttgaatagg
     1981 ttcgttacgc aaagctcctg cacaccatag ttcattgtag ccaagtggag tggtaggtgg
     2041 ttcgaaagtc gtgccaatgg caattgctgg atctaaacca atattgatag tgataggcat
     2101 tggtttattc aaggcttcga attgtttttg gaagtgacca atgtggcgtc cgccaggcat
     2161 gatgtacata ccgatcgtat ctttatcttc tagaaccata cgatggatcg ttacatcgct
     2221 cattgttttt tctggatctg atcctaagac tacacccatt gtgatgtatg gaccagcatc
     2281 ttcaggagta tttgtaggtg caggcaaaat gttgcgaata tcgaagtctg catcggtagc
     2341 tagatgaacg acttcttgtg ctggagcgtc tgctttgtct actttcacag gtttgattgg
     2401 attttctaca gcgtctttca agaaacgacc aagtgttcga taatcatggt gcaaaatttc
     2461 acctacacgt ttacgactag ccattgttcc gatgttcaca cgtgtgccgg ggaaaccttt
     2521 gatattgttg aaagtcatag ttggtccttc ttgagtcgga cgttttactg taccgccagc
     2581 accgatataa cggtaaacac cagataattc tgcttcagga tcgatttcaa tatctgtttc
     2641 gtgatattga cctggaagtg tttttagttc ttcgatgact tttcttaaat catacggttg
     2701 atctgtcatg agagattcct ccttagttgt ctttacctat ctatagtaaa aggtgaaaaa
     2761 gaggaaaaca attcaacttg ttcggtaaag tattctacaa tggaatggat gcgttttttt
     2821 gataatagaa taaactatga tatacttatc actaaaagaa agcgcatact tagtgttgga
     2881 ggaaggaaag tgaaaaaaat ggaggagtca tttcctaaag cagtaaaagt ggaaaatata
     2941 gcgaatattt tgaaagttac atttgaaaac ggagaagtaa aatacgtgaa atctcattgg
     3001 acagaagaga taaccgatgc cttgcaattt ggaaaaaaag gaagaggaaa aagaaaaaat
     3061 cttctcgcac tttctcgtaa tatgtggatt gggactgaag taacgattga agcagatgga
     3121 acagtattca taaatggaaa agacaggtat acgccagaag agttgtggta caaaggaaag
     3181 aaaagtatac cagaattata aaaaataatt aaaaaaaaca gcgcgaaaca aggaacatat
     3241 tacttcctcg tttcttgctg ttttctacta tttcagcgct cttatgactc aaatctaaat
     3301 gtggttgtca caaccttatt ctgcacgcaa agcgatagcg gcgtcttttt tcgctgccat
     3361 tcgtgcagga agatgaccgc caagcatcgt caatatagta gagacgatca ctagaataat
     3421 cgcatggaca ggatttagtt gagcaacatt ttttagatcc gtcatgttgt ataagatgct
     3481 attgataggg aacgtagcta gccatgcaat aaacacacct aatatcccag aagaaatacc
     3541 taaaatacac gtttctgcgt caaatacgcg agtgatgtct tttttacgtg cacctaaagc
     3601 ttttagtaca ccgatttctt ttgtccgttc aatcacagat gtataagtaa tgatactgat
     3661 catgatcata cttgttacta aagagatccc tgcaaaagca ataaggacat aagtgatagc
     3721 atccatcaat ccgccagtca gttcagtcat cgttccagct aaatcagtat aaatgatttt
     3781 atcttcatct gatttgcctt tattgtaatc atctagataa tccaagatct tttctttgtc
     3841 ttcaaaattg ttcggataaa tcatgatact tgaaggcaag ctgtctccac ctaaataact
     3901 aagcaatgtt tgtttggcat tttcgtcaac tttttcagta gttaagacat ttacaccact
     3961 gtctctttga gctttgacga tttctgattc tttgttttcg ttgacgattt gagtcgttaa
     4021 ttgatcgcta taagcgattc ctggagataa gagattcatt gtagaagaag atttcacgcg
     4081 taggatcccc gagatcttca attcatcaga agcattctga tacatcgcat cataatctgt
     4141 attagggata aaattcccag ttggaagttt tgtatagtaa gtattgttgt ttactaattt
     4201 gaatgttgta ccaacgatat catcaaaatc aagtttttcg tcttctttca catcaaatcc
     4261 aagattcttc aatgcgttga tattagtatt attattgccg tctacgatca gcacgacatc
     4321 tgttgctgag gctggataac ttccggcaag taaagaataa ttgtctttta aaaagttttc
     4381 tttgctagtg tcaagctgtg tagggaaact tgacactccc acacctgtca ttgctgacat
     4441 cgtactagat aaagaaagag attccgtatc aggattttga ttcgagaagc tgacaggctg
     4501 tactttgcca tttacatcac gtaaaagatt gattcctgta gtacgggtaa atccgatatt
     4561 attacttaag ttaggatcga tgtctgttac gtaatcgacg tatgtttgat caatcttatt
     4621 cgtgtgctgt gctttatctt cttcgctgac ttttgcagta atcgttttgc tgtctggata
     4681 atccgctttg cttgcaccca atccggcatc gtcacgagtc tggttagtcg ttaccttaga
     4741 aatagtaatc ggaaatttcg ccatcgtttc agcttgcgtg ttatcgattt gtttttggaa
     4801 tccagaagaa agggacagaa cgatcgcgat tccgatgatc ccaatactgg aagcaaagga
     4861 ggtcaagaaa gtacgtcctt tttttgttcg gatattgttg aaggaaagct ttaacgctgt
     4921 ccaaaaactc atctttgttc tgcgtaagtt aaattgatca tcttttggtc gttcgatgtg
     4981 aggatgggaa tctgttaaga tcttaccatc agaaaattca atgattcgat ctgcgtattg
     5041 atgggccagt tcaggattat gtgtgaccat gatcactaat ttttctttcg ataactcttg
     5101 gatcaattcc atgatctgga tacttgtttc tgtatctaac gctccggttg gttcatcgca
     5161 caaaaggata tctgggtcat tggctagtgc tcgagcgatg gccactcgct gcatttgtcc
     5221 accagataac tgatttggct ttttatgcat atgatttgtc aagccgactc ggtgcaatgc
     5281 atcttctgcc cgttttcgtt tttcgtcttt agaaacacca cttaatgtca tcccaagttc
     5341 aacgttttca ataatgccta gatgaccaat caagttgtaa ctttgaaaaa tgaaaccgat
     5401 agagttgttt cggtaggcat cccagtcact gtccttaaag tctttagtag actttccgtt
     5461 gattaccata tcaccggaat cataattatc tagtccacca atcacattca gcatagtcgt
     5521 tttaccagaa ccactaggac ctaaaatcgc tacgaattct ttttgtctga aagcgacaga
     5581 cacaccatcc aatgctttgg tggtggtttc gccgacttta taatattttt ttatatcttt
     5641 taattgaagc ataatcaccc ttctcccgta ttgaaatatt cggtatacta ataagtatat
     5701 cagaacaatg atcgtagtat acgaaataaa tgttaactga agatgaacta caagaaaaaa
     5761 tctagaaatt tttcaaaaaa cagagggaga tcaatgaatc gtattttagt agtagaagat
     5821 gatccgcaat tacagttgct ttaccgttct gtgttggaac gagcgggctt ttcagtcatc
     5881 accgcattga atggagagga tgcgctgaaa aaactagaag cgacacgtat agaattaatc
     5941 attacagata tcatgatgcc gaaaatggat ggctatgact tgctagactc attacgtagc
     6001 accagatttg acacacctgt tctaatgatt acggcaaagg ccgattttga ggataagaaa
     6061 cgcgggttca aattaggagc agatgattat atgacaaaac ccatcgatgt caatgagatg
     6121 atcctacgtg tggaagcatt gcttcgacgt tcaaagatca attacagcag tagcttgata
     6181 atcggacaaa caaaattaga gctagatgct tatacagtgt tacaagatca gcattcgttg
     6241 attctgcctc aaaaagaatt tctgcttctg tataaattac tgtcttatcc caacaagatc
     6301 ttcactcgcc aacagttgat ggatgatatt tggggattag atacgaatac agaagaacga
     6361 acgatcgatg tacatatcaa acgtttaagg acccgttttg aaaagacgga agattttcaa
     6421 atcatcacag tacggggact aggctataag gcggttattt aaagatgaaa aaaaagctat
     6481 tttcacaatt atggatctat tttgctagta ttgtcttcat ttcgatctta gtgacactgc
     6541 tgtgttttct cggttttatt ttcttattgt ctcgacattc tatctcgcct gctgaatcgg
     6601 ctggaaaacc aacgttatac ccgttattcg tctttgcggg gttcagtatg gtcgtgggta
     6661 caggaatatc gatttttgtt gggcggcgta tcctgcatcc aatcagtgcg ctagggacaa
     6721 atatgagtct ggtagcaacg ggagattttt cgattcgaat ggatgaacag cagaaagtag
     6781 cagaagtaca gcaactgtat aaagatttca acgttatggt ccaagaactc aatagtatcg
     6841 agacactgag gaatgacttt gtgtccagtg tatcccatga attcaaaaca ccactagcaa
     6901 caatccaagg atatgttcaa ttgttacaag ccccaaatct ctctgacgaa gaacgccaaa
     6961 tatttttata tcggattatc gaaagtatca cgcagctttc acaattaaca gagaacatct
     7021 taaaattgaa caaactggaa aaccagcgta tccaactgga aaagaaagag tatcgattag
     7081 atgaacaaat cagagaagta atcgtttttt ttcagcctaa atgggagaaa gaacagctag
     7141 aattggatat cgaacttgct gcagtaaatt acacaggcaa tgaagagttt ttgtaccaag
     7201 tgtggctgaa tatcatggat aacgccataa agtacaatca aatcaatgga cagattcata
     7261 tcaaactatt tgagacagcc acagagattg tactggaagt gaccgattca ggtgtaggaa
     7321 tgaatgaaga gaccagagat cgaatgtttg aaaaatttta tcaaggagat acaagtcgtc
     7381 aaatttctgg gaatgggcta ggactttcac tggttaaaaa aattctagaa ctgcatgatg
     7441 gacgtattga ttatagcagt atcgaaggag taggaacgac agcaatgata cggttaagta
     7501 aacaatagga aaaagaataa aacccctaaa aattctattg aaggttttta ggggttttta
     7561 gtcgtttttt tgtgattagg gaaatgttct tacggttctt ttggcataaa aatcgctgct
     7621 ttcttattga actacgagaa atgttagagg tgtcattgaa acaaggacat gatgcaagaa
     7681 agagagcgaa agtcatgcgt cgctctcttt ttttgggtgg atagatggac agaaaatgta
     7741 gggcttagtt ttcttcttga tttaaatact cccaaaatgt atctgaagcc aaagaatgct
     7801 caccgacttt acgagtaaat gcaagataac tctctgttgt tggagaaata gggagaatca
     7861 cagtattttc ttttaataga ttcttgactg ttttttccat aacaatagca atccccatgt
     7921 catttgcaat catattgatg atcaagtcga ttcttgctcc tttataagag atttttggtt
     7981 caaatccagc ttcgcgacac agatctagta caggttgata caagttcgta gtggggccaa
     8041 ggagcaaaaa gttctccttt ttcagttcgc ttagatccaa tttcttttta ctagctaaag
     8101 gatgattttt aggtagaact gctacaaaat cgtctgtttc agtaggcaac cactcaatcg
     8161 aacttggtgg ggcctggaaa cttctagcaa agtggatcgt gtcactatgt tcattggcgt
     8221 cattgtaagt taattcgaca ctttccactt ctttcaactg gactgtaaat tcaggatggc
     8281 gtttcaaaaa ctcggtgatt ttggaaaaac ctttataatt gatcattgtc ggtatcgtta
     8341 agatatttaa tgaaagattg ttgtctttcg tataggtttc aagcacttct tgcatagcat
     8401 ggtattgttc tacgacattt tttacatatg ggagcaataa gcttccagct tcggtcaatt
     8461 cgatttttct atgggcgcgt tcgaataatt tgacgtctaa ttctgtttct aacgaaagaa
     8521 tctgtttaga aatatttcct tgggtggtga attgtcgatc agcagttttg gtgaaattca
     8581 ttgtttcggc taaatcaaga aaaacttcga atttatggat atccatcata ttcctcctac
     8641 ttattccaat aaagaatact ttaacacaaa aaatgaattg gtgtaatctt ctctgtctgt
     8701 tacaatcttt cttgcagcga aagcaataat tacagaaatt tttctgattt ttttcgcttc
     8761 gtagaaaaag agaggagttt tttgatgcca actggagttt tgatcaatgt aggttccgtt
     8821 ttgtttggcg gactaattgg cggattagta ggaaataaat taagtgaaca ttttaaagca
     8881 cagctgacga tggtttttgg tgtgtgttcg atggggatgg gaatctattc cattgcgccg
     8941 atgaaaaata tgccggctgt tatttttgct ttagtgatag gaacagcgat tggtttgatt
     9001 gttcatttag gtaatgggat aaataagggg gctgcgctga tgcaagtacc tatttctaaa
     9061 atcttcccta gtgaaaagct tggtatgacc cacgatgaat ttatcagcac attagtgacg
     9121 gtcattgttc tattttgtgc aagtggtacg gggatctatg gaagtttaga ttctgggatg
     9181 acaggagaca gtacgatttt gatttctaaa tctgtccttg attttttcac agcagctatt
     9241 tttgcgtgta atttagggta tgtcgtttct gtgatagcaa ttccccaatt cattattttt
     9301 tatattttgt tcttattggc aaaatttatt tatccattga ctacaccaga catgatctta
     9361 gattttaaag cttgcggcgg attcttgatg gtcgctaccg gttttagaat gatcaacgtg
     9421 aaaatgttcc cagtagctga catgatacct gcaatgatcg ttatcatgcc actgagctgg
     9481 atgtggacaa attggattat gccactgtta taataaaaat caaagagaag ccattttttg
     9541 ctgactaaaa aggatcaaat cttttagtca gtttttttgt atttgacaaa ctgattttga
     9601 aactatatta tatgttgtat aatatagatt gaaataaaat attacacttg agaatagaag
     9661 gtgacgtaat gcacatccag attccaactg ttcttcttga tggtactgtg ctagcagcat
     9721 taagcaagga agatatgtat gggtatgcct taacaaaaat cgtacaagaa agtctctctg
     9781 tcagtgaatc tacactatat ccggttttga gaagattgaa aaaagaagga tatcttgaga
     9841 cctatgatga accttttgaa ggcagaaacc gtcgatatta ctcgttgaca ccaatgggaa
     9901 gtcgtcattt agaagaaatt attgatgaat ggattgtttt tcgagattct ttagaccggt
     9961 tattggagga gagcacaaat gaatgaatat ttgaatgaag ttgtggatca tcttcaagaa
    10021 attcctgaag acgatcgttt tgatttgatc cagtattatg aggaatattt tttagattct
    10081 ggaaaaacat tagatcagat cattgatgaa tatggtacac caaagcagtt tgccttaaaa
    10141 ttaaaaatca gttacttttc agaagaagaa caagcagaaa acgagttagt gaaaaatcca
    10201 aaaagacaaa ttcgcctggt ttggttaatc attatcggtt tatttgcttc tcctgtcctc
    10261 attccactag cactggggtt tatattggtt attgggggac tattggttgc tttactagcg
    10321 ctcattttta gtatctatgt tttatgtatc agtatcttag gtgttggcct attcatgatc
    10381 atctcaggaa tcagtgtgtt tggacaatcg attctgagtg gattattctt tatcggtgtc
    10441 ggtctgttcg ccacaggaag cgcagtattt atttcgccac tgttgctgaa aggaacaaaa
    10501 gggctttttt atgtcatgat gaactttgtt aaatggatgg gacgacgttt tattacaaaa
    10561 agagatctgc attcagcggg cgtttgaagg aggacacata aattgagatt gaaacatttt
    10621 ttgattaccg gtattggtct gatgcttact ggtggcctcc tagcaggtgc aacttataca
    10681 ttaggcgctc aaaagagtct gacttgggat aatggtcctc ggttgatcga tatggttact
    10741 gagacaaaaa aaatcaagga tgcaaacaaa atcgtcatcc atgcagaaaa tcaaacagtt
    10801 tcggtcatac gcggattgga ttttgagata aaaacgactt atgataagac agaaaagcca
    10861 gaaataaagg agaaaaatgg cgtgttgact gtgaatgcag tcaaaaaatc aacaggtgca
    10921 atcattagtt tagaagacac aagtgcacaa atcactattg ttgtccctgc agatcatgaa
    10981 ttatctgaat tgacaatcga agggaataat agtgcaattg atatcaacga agtatcaatc
    11041 aaaaaagtga atactaagac acatgatggc tgggtatcgt tcaatgaatt tacaggagga
    11101 acaataactg cgaaagatga attaggctca cttcgattag atgaggccaa tgtggataac
    11161 ttgatcgtca caggtgaaaa cagcaacttc catctagaag acagtttgat cagcaaagaa
    11221 ggaaaagtca tcctaaacgg tggcaattta tctttgcatg agatggaaaa cagtggctat
    11281 actatccaaa cgtctggtga atcttatatt gaaaaagact accagccaat aacaaatact
    11341 ttgttagaac aaggacagaa aaagctaaag atcactgcta aagacacatc gatcaatatt
    11401 acgacggatc aagaggacaa tgaaacagat tgatcctgta ctcaactgga tattttaaga
    11461 aaaaacagca aagatccttc caatctttgc tgtttttcat cgcttaacgc acataactat
    11521 agtaaaataa gaaagaaaat atctaaaaag aaaacagggt gaaagagtat atgaaggtag
    11581 aagaaggtca aagagaaaag ttaaaaacag aaatggaaag attacatact tatatcactc
    11641 aactaagcca aaccttctat gatccagaca aagaaaaagt gatggttaat tacccgaaca
    11701 attcagaagg tcgccaatta gagcaagtgt accatgaagt tttcaaacat ttattgacgg
    11761 tgaagaaaga gctggattat tattctttac cgattatcga tacaggaata ttgaagtatg
    11821 atgggaagaa ggaacgattt atctttaaat ctgttcggga agatttgcct ctttcagcag
    11881 ggatggatct agaggtcttg gtagaagact attttacaga agaaaagcac tgggttcgaa
    11941 caaaattgga ttatttaccg caggcagccg gagggacaca ggcaagcggt tggtacatca
    12001 ctgaagacaa agagttagaa ctagaaggtg taatggcgcg tattcgaaaa aaaaattaaa
    12061 aaagtaacat tagatattta ttgaaggata aaggagcgca ggaagtagca tgagagaaaa
    12121 aagagcattt tatatcaatg gttatgcagg gattgtcgga ttggtcgtat tagctttagt
    12181 tggattgttt ttcttctatt taggtatgtg gcaagcgaaa gtttgggcac tttttttaag
    12241 tatttttctt tggttgatca cgttattgtt gttaagttcg gcaactgtag tcagtcctaa
    12301 tcaagcaaaa gtaattttgt tttttggaca atatttaggt acgattcgag aaaatggctt
    12361 tttccttacg atacctttgg cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa
    12421 tagttctgtt ttaaaagtca atgatttgga tggaaacccc atcgaaatct cagcagtagt
    12481 agtctttaaa gtcgttgata cagcaaaggc attattcgat gtagcgtatt atcaagattt
    12541 tgtagaaatc caaagtgaga cagccattcg gcatatcgct agccaatacc cgtatgatac
    12601 attcaatgag aatgatttga cgcttcgagg aaacacgacg gcagtgtctg acgagttgca
    12661 aaaagagctg caagagcgtt tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa
    12721 ccatttagcc tatgcaacag aaatcgctag tgccatgctc caacgtcaac aagcaaaagc
    12781 cattttgtct gctcgacaaa ccattgttga aggagctgtc acaatcactc aaatggcctt
    12841 agagcaaatc gaagatggac aagaaatcaa tttcaccgat gatcggaaag tccaattgat
    12901 caataattta cttgtctcaa tcattacaga caaaggaacg caacctgtta tcaatacagg
    12961 cgacatgaaa gaccgtaagt gacatacttt ttgtaagata ggaagaagga gtttaggacg
    13021 gattattgtc ttgaactctt tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag
    13081 aatgatagtt gattcgaagg cagaattgtt cgcttttttc tataatcagt atataaagta
    13141 agagagaagg ggaaaaagtg aaaaaacttc tgatcggtct acttgttttc atcgttgtgg
    13201 tacttgcagg ggtgaaaatc gcagattacg tagttatggg tggagaagaa tactatgtac
    13261 aaattacgac agatggtaca aaaacagttt caaaatcaga ctctggacaa gaaacagtca
    13321 actatagata tatcctgcct ggctatacga aagagggtaa ggaaaaagaa ctggacttca
    13381 agggacaatt gactcgaccg cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa
    13441 ataaaggtgt cacttcgtat gaagaagtcg aaaaaaaaga cgtgccgaaa acagcactgg
    13501 aaaaactaat taaagggtag ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac
    13561 aaaagtatac ggaaaaaaca atgagaaaaa gacgcaagca ttaagcggca tcagcttcga
    13621 tgtagaaaaa ggcgaattta ttgggatcat gggcgcttct ggttctggta agtcgacact
    13681 tttgaatatt ttatccacat tagataaacc aacagatggg catatccgaa tcaatcaaaa
    13741 agatgtgacc acattaaaag gaaatcaact agcagatttt cgtgcgaatg agattggctt
    13801 tattttccaa gatttcaatt tattagaaag cttgacagca caagaaaata tcgctgttcc
    13861 tctttcttta caaggcgtca gaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga
    13921 acgtttatcc atctctcata ttttggaaag ctatccttct gagatttccg gtggacaaaa
    13981 acaacgggtc gccgccgcac gtgcattgat cacacaacca acgattctat taggggatga
    14041 gcctacaggt gcgctggatt ccaaaagtgc ccgggatctt ttagatacga tggacgaatt
    14101 gaatacaaaa gatcatgtat cgattcttct tgtgacgcat gatccgtttt ctgcaagtta
    14161 ttgccaacgt attttattca tcaaggatgg cggtatccat caagaagtga aacgtggaga
    14221 tcaaagccgt gattccttct atcgagaaat actatctatt cttggaaact tggaacagta
    14281 gggggagatt gactatgctt tggaaattat cacttactgg gatcaagagc cgtttaagag
    14341 actacttggt gttattctca ggattagtta tggcatctgc tattttttac atgtttgaat
    14401 caatggcaag caacgaagct tttttgaaaa gtaatacgat cgttgcggct acagttatca
    14461 ttttccgatt cggttctgtt ttactaggga tcatcacatt cgtttatatt ttatatgcaa
    14521 attccttttt gatgacgatg cggcaaaaag actatgcaat gtttatgatg ctgggagcaa
    14581 aaggcagaaa aatcgcacag atgatttttt ctgaaacatt cttagtaggg atcgcagcta
    14641 ctttagtggg ttcagctgtc ggtgtaggat tgacaagtat cgtccaccga ttattagtgg
    14701 atcagttaga tctcaatatt tcgcatttta caccttttag tattcacggg ttgttgatca
    14761 ctgttttgtt ttttgctgtt ttgtttttat tagctgctgt tgtcaatgct ttttcgattg
    14821 tcaaaaaacc gattttagca ttgattcgtg cggatcagac accaacaaga atcaagcagc
    14881 ataaattctt gtttttcctt gaagtagtac tgggaattgc ttttctaggc tgcggttact
    14941 atatgatgaa acacgtgggg acctatcaag tctttggtat aggggtagcc ttagttacga
    15001 tcgtattagg aacttatttc attttccatt ctgtgatcat tttcttttta tcattactaa
    15061 agaaatcaga acagatttca ttgaaaaaat tgaacaattt cacattgtca cagttaaact
    15121 ttcgtattcg tgaatataca caaatgctct caatggtcgc tatgctgttt gccttggcac
    15181 tgggcgcttt gacggtagga ttaggtttta aaaatgaaat tgcagacttg acagaaaaca
    15241 attctggata tgatctagtg atgaataatc ctcgggcaga agatcagcaa aaaatcaaag
    15301 aactttcacc aacactttct gcttcttata ctcaaaaaga agatgcggga acgatttact
    15361 ataatgcaag tgaatttgcg gctcaaccat tgatcatgat aaaacatgaa gatggaacac
    15421 cgccgaagat cacgaaagaa aaagttcctg ctaaaaaaat gaatgaactg actgtacaag
    15481 aggaacttcg aagctatgaa ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc
    15541 aatcagaatt tgatcaactg aacttgccac aatccacttt acagctagtt caggtaaaag
    15601 acttccaagc aaacctgaaa ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc
    15661 catctttgca aaaagtagag tacagcaacc agaaatatga aatgtatgaa gtgtataatg
    15721 gtctattctc tggttttgaa tttatgggat tcttcctagg tttagctttc ttgaccatgc
    15781 ttgctagctg tttgatgttc aagatcttgt caggaagcaa gagtgacatt gctcgttatg
    15841 aaatgcttga aaagatagga acacgacgcg ggttgcttaa acaatcgatc cgtagagaag
    15901 taggcgtgtt attcttagca ccaggaatct taggtgtagt ccatgtctta ttcggattac
    15961 aaatgtttgg tctattcatg caaaatccat acaaagatat ttgggttccg tttacgatat
    16021 tcttcgtatt gtatttcatc tactatgtac tgacgacatg gttatatact ggaatcgtat
    16081 tgaaa
//
LOCUS       DACENJ010000048        15929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.048, whole genome shotgun sequence.
ACCESSION   DACENJ010000048 DACENJ010000000
VERSION     DACENJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.048"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(20..904)
                     /gene="rsmA"
                     /locus_tag="IW748_001105"
     CDS             complement(20..904)
                     /gene="rsmA"
                     /locus_tag="IW748_001105"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1104025.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="IW748_001106"
     CDS             complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="IW748_001106"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1104026.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1459..2235)
                     /locus_tag="IW748_001107"
     CDS             complement(1459..2235)
                     /locus_tag="IW748_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1104027.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2390..3712)
                     /locus_tag="IW748_001108"
     CDS             complement(2390..3712)
                     /locus_tag="IW748_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1104028.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            3887..4474
                     /locus_tag="IW748_001109"
     CDS             3887..4474
                     /locus_tag="IW748_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104029.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(4953..6962)
                     /gene="metG"
                     /locus_tag="IW748_001110"
     CDS             complement(4953..6962)
                     /gene="metG"
                     /locus_tag="IW748_001110"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1104030.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            7230..7508
                     /locus_tag="IW748_001111"
     CDS             7230..7508
                     /locus_tag="IW748_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104031.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            7605..8426
                     /locus_tag="IW748_001112"
     CDS             7605..8426
                     /locus_tag="IW748_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1104032.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8711..9721)
                     /locus_tag="IW748_001113"
     CDS             complement(8711..9721)
                     /locus_tag="IW748_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1104033.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(9733..10686)
                     /locus_tag="IW748_001114"
     CDS             complement(9733..10686)
                     /locus_tag="IW748_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1104034.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(10676..11746)
                     /locus_tag="IW748_001115"
     CDS             complement(10676..11746)
                     /locus_tag="IW748_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104035.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(11752..12795)
                     /locus_tag="IW748_001116"
     CDS             complement(11752..12795)
                     /locus_tag="IW748_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1104036.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(12796..13737)
                     /locus_tag="IW748_001117"
     CDS             complement(12796..13737)
                     /locus_tag="IW748_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1104037.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            13821..14168
                     /locus_tag="IW748_001118"
     CDS             13821..14168
                     /locus_tag="IW748_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1104038.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(14238..15899)
                     /locus_tag="IW748_001119"
     CDS             complement(14238..15899)
                     /locus_tag="IW748_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1104039.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         4481 a         3296 c         2863 g         5289 t
ORIGIN      
        1 tctgtcacaa cctctttttt tactgcatat tttctgacat cgcattgctt aatgcggcga
       61 attcttgtaa tgacaaagtc tccccacgac gttttggatc gattccagct gtttccaagc
      121 tttttgctaa ccatgctttt gtctggtcat ctttgccata cgaatgctgc aagttattcc
      181 aaagcgtttt tcttctttgc tgaaatgccg cttttgtcaa tcggaaaaat gctttttcgt
      241 ctgtcacttc gactgccggt atatctcttc ttgtcagttt caaaatcgct gaatctacat
      301 ttggctgagg gacaaatact gtttttggta cgatgaatgc taaactggct tccatataat
      361 attgaacagc aatcgataat gaaccataag ctttcgttcc tggttcagca gagatacgat
      421 cagctacttc tttttgcatc atgacgacta gttcatccac aaccaaatct gattcaagaa
      481 aatgcatcat gatcggtgtc gtaatatagt aaggtaaatt cgctacgact tttaacggaa
      541 gctcctcatg aaatgtttct ctaacagctg ttgacaaatc ggtctttaaa atatcttgat
      601 gtacaatagt cacattgtca tagggttgca tcgtatcagc caatacaggg atcaaacgat
      661 catcgatctc aaatgctacg acttgttttg catgcttggc taattgttcc gtcaatgcac
      721 cgattcctgg tccaacttcg atcacgttcg tatgttcatc tatttgcgca gtttctacga
      781 ttttccttaa aatatttggt tcagtcagaa aattttgtcc taaacttttt ttaaatgaaa
      841 aaccatgttt caataaaatc tcttttgttc tagagggtgt cgcaatttca cgatattcac
      901 tcatgttgtt cctccactat tttcattgct tgggcgagtt ctttcggcgt aatacgaaac
      961 atcttcaaac gtttttccaa ttgtttgccg ttcgtgtaac cgatgcgcaa ctcgtcacct
     1021 agtttttctc gtttttcttt tgcttgtgcc ccagctatta aaccatagtc taaaaggtct
     1081 tgtctagtga tttcagaaac agccgtttgg gaaatgtctg gcgtcacgac ctttttcaat
     1141 gcttctaaaa tagcagcatc acttgcatgc tcgacaccaa ggcttccctt tttctttggt
     1201 ttagcttgtg tgcgcggcaa aaaagcatgt tttgcctgag gaacagcttc cataatgatt
     1261 tttcttattt tttctcctga aaaatcagga tcggtaaaaa cgatcactcc acgagtctct
     1321 tgggcatgtt caatttgcat caggatctct tcattgattg cagaccctat tgtttctatc
     1381 gtatctgcat caactacttc ccgcagcctt cttgtatcgt cttttccttc aacgactata
     1441 atctcttgga tcttcatctt atccatgttc gcttagacca aataatcggt gcgcatttgt
     1501 ccatgtttgg gcagccactt cttctgtcgg aagattccga agttcagcaa tcttttctac
     1561 tgtataacga gtatagcctg gttcatttcg ctttcctcta aaaggaactg gtgccagata
     1621 aggggcatct gtttccacaa gtaatcgttc gattggtaca tttgctgcag cctcttggac
     1681 gtctaatgct tttttgaaag tgacgacacc gctaaatgaa atatgcatcc ctaagtcaag
     1741 aaatcgtttt gcccattcat aatcaccact gaaactatgc atgattccac cgatatctcg
     1801 aacatcttct tcttttagga tttgatacgt gtcttcaagt gcttctctag tatgaatact
     1861 gattggtagg ttcatttctt ttgcgatagc aatctgtctg cgaaatactt tatcttggac
     1921 ttctttcggg tcttccatcc aatgataatc caagccaatt tcccctaatg ctacaacctt
     1981 tggtaaagtc aattgttctt gtaatttatt ttcgatttct ttcgtataac tgccagcctc
     2041 tgtcggatgc cagccgataa tactgtatat atttggatag tcatggctta attgcaaaga
     2101 cttttcaatc gttggcgtat cgaagcccac gaccgccatt ttcgttaccc ccatttcttg
     2161 tgctcgctga atcgtttctg gaatatcttc attgaactgt tcagcattta aatgggtatg
     2221 agaatcaaat atcatgttta tctctccttt tcattcttac tttagcacat aagcactact
     2281 tgcagaagta gcaaaaatca aaatagaaga acttgtttta ttgaaataga aaaagcacga
     2341 agatctagag gacctagttc gtactttttc tgtctgttgt tgaaatattt tactcttctg
     2401 agcgtttttt ctttttttga taatagactg ggatacctat caaggtaaca cctataccga
     2461 tcaatgctaa tttcgtttgc gtcatcatcg tcatacctaa aatgaaaata gcaccgagaa
     2521 tcgcaataat cggaacgatt ggataaagtg gtactttata tggacgtggc aattccggcg
     2581 ctttttttct taaaataaag acaccgataa aaatcagtaa actgaatagc cacatcacaa
     2641 aaatcaacat gtctgttaaa aagtcgaaag aaccaaggct catcataata caagctactg
     2701 ctaactggaa aaccgcagga acataaggaa ccgtaaactt ctttgaaagc tttgtcagct
     2761 gtttactgaa aggtagctcg tcttctaaag ccatcgcata aggcacgcgg atcccagtta
     2821 gtgtataacc gttcaatgca ccgtataccg agatcaagat cccaatagtg accagctttc
     2881 caccgattcc tccaaaaatg acgtctgatg cttcagaagc tgcattcaaa ttaccggcaa
     2941 gatgatcaat cggcaaagtt ttcaaaaaaa caaaattgat caatagataa acgactgtta
     3001 caaaactcaa accaagaata attgctttag gcaaatcttt ttccggccgt ttcatttccc
     3061 ctgccattgc accgacacct aaccagccgt cataagcaaa caacgttgct aacaacgcac
     3121 tgctcaatcc ttcgacaaaa gtaacatctt ttccagcttc gattggaaat aactggacag
     3181 ttccttgtcc tggggtcaat aatccccata tgacaatcac agcaattggt attaatttaa
     3241 caatcaacgt cactgattgt acagaagcac cgacttttgt ccctaacaaa ttcaaccctg
     3301 tcacagaaac agcggtgatc actgcaatca acaaaagata atcggtagat aattgtaata
     3361 agttcgtcaa ttgtgtcgca aagataatcg ataatgcagc aatattagca ggaaagtaaa
     3421 tcaagctttg cgcccatcct aataaaaagc tgggcaattt tccatacgca gcttcaatat
     3481 attttacagc tccaccagtt tccgggattg ctgtagctaa ttcagccact gtcaatccag
     3541 cacagatcgt taagaaacca cccaaaagcc aagctagcaa tgtcaaactt gtagactgag
     3601 tactagcagt aacagcagca gttttgaaga agacgcctgc accgatcacg gttcccataa
     3661 cggtagctaa tgcgccaaac agattgattt ctcgttttag tttgcctttc attcccatcc
     3721 tctttcttct ttgaaatttg gtataatttt cagtataact gagaatattt taatttacca
     3781 ttcttttttc tccactttca gtgaaaaact ccctcttaag acggatgtat ttctttttta
     3841 tttttcgtat actaaaccta ccttttccac gaaggaggaa cagtttatgg attttaagcg
     3901 ttcttatccg aaattagcag attatttatc caaccgggtc gatttttccc gtaaagaaca
     3961 acagcagtcc cattggcatt ggttcacatt aattttattt ggattaatga ctgttgcaac
     4021 gttatcacaa aaactaatat tgctgacttt gttcatcgca atcgttgcat tgatcaaagg
     4081 tcctgtgatg atcttgtggg ggatgttgta ttcctttttg gttgggcttt ttccgcctat
     4141 cggtattttg ctttcagcga ttttcttttt attgaatatc ggaacattta caaagaactg
     4201 gcggatgacc ttagtcggga tctattttta tttctatcct tttggagtga tggcacttag
     4261 tgaagtcatg cattgggata atcattggtt tatagctggc tccttgcttt tagggttgat
     4321 cttgttacac gtgatgttga caaagctcta tcaacattat ggtatcggac gtacgatttt
     4381 ctggtatgtc ttttcgatcc catttgcact gcttactgct ctcttaccaa gccgtttgaa
     4441 gacaaaaata aaaggttacc ataaaatcaa gtgataggat ttttacaaaa taaagaaaac
     4501 aatcatcgaa gtcaaagccg atgattgttt ttttatctat attttcaatc acacatataa
     4561 gagcacaaac taattgaaca taaagtgata gaacttataa ttaacacatc aacgctactg
     4621 ttagatataa atatagatgt aataaagtca ttgcattcaa actttatgaa tgtaaggaca
     4681 ataaaaaaga gtaccagatg aatcaattaa aatctgcccg accctttatc attatgcttt
     4741 ttttctgttt ttacgattcc tattagtatt attctaaatt aacaaaaatc agcgaagctc
     4801 gtacttcttt ttctaggatt tattttggat agttctctgt ttctgtatta tatgattggc
     4861 ttcaagtaat aagaaggaat ttacgaaaat tgcttctcaa atttcggaaa aagggggtat
     4921 gacacaacta gtgtcatacc ccctttgaat ttctatgcga tttctgagcc atttggcatg
     4981 gattttggtg cttcgattac ttgtaaagag ccatctggtg cttctgcaga aaggatcatt
     5041 ccttgactga tttgtccacg catctttcgt ggtttcagat ttgctacaat gacgactttt
     5101 ttcccgatca attcgcttgg atcaggataa aattcagcga ttcctgacaa gatttgacgg
     5161 tcttggctgt ctccagcatc tagtcggaat tgcagcaact tatctgcccc ttctactttt
     5221 tgacagttga tgacttctgc tacttttagt tctacttttt caaatacatc aaatttgatt
     5281 tgtttttctt tcgttgaaac aagttctgtt tcttctggat cccatttgac tgtatcttcg
     5341 tttgtttgag taccttcaga cattttttct tgaataaaag caacttcttc ttccatatct
     5401 aaacgtggga agattggtgt accttttgca actacttttg ttcctgaagg aaattcgcca
     5461 aagtgtaatc cttcaagatt cattgtttca gagtccaatc ctagttgatt gaagatcttc
     5521 tctggcgttt ctgtcatgat tggctgaagt aatatcgctg cgatacgcaa agattcagct
     5581 aaatggatca tgacactatt caattcagct ttcttttctt cgtcttttgc taatacccat
     5641 ggttctgttt catcaatgta tttgtttgca cgagaaatca gcacccagat ttcagccaat
     5701 gctgtattga attccatttt ttccattgca tcatggtaac gtccgacaac gttagcagcc
     5761 gtcgtagaca gttcactatc aaatggagtg acaagtgagg catagtcagg aacgagtcca
     5821 tcacaatatt tgttgatcat agcaatcgtt cgattcaata agttccctag atcatttgcc
     5881 agatcataat tgacacggga aacaaaatct tctggcgtaa agacaccatc tgacccaaac
     5941 ggaacagcac gaagcaaata ataacggagt gcatctaaac cataacgttc cacaagcatt
     6001 tcaggataaa cgacatttcc ttttgattta gacatttttc cgtctttcat caatagccag
     6061 ccatgcccaa atactttttt aggtagaggc aaatcaaggg ccatcagcat gattggccaa
     6121 tagatcgtat gaaagcgaac gatttcttta ccgaccatct gaacatctgc aggccagtat
     6181 ttttcgaata gactttcatc ttcggagcca tatcctagtg cagtaatata attggataat
     6241 gcatcgatcc atacatacac cacatgtttc ggatcactct tgactggtac tccccaagta
     6301 aaagttgttc gtgaaactgc aagatcctct aatcctggtt tgataaagtt gttgatcatt
     6361 tcgtttttac gagattctgg ctggatgaat tcgggatgtt cttcatagta ttccaataga
     6421 cggtctgcgt atttgctcat gcggaagaag tatgattctt cttttaccaa ttctacttcg
     6481 tgtccacttg gcgctttacc accgatcacg ttaccttcgt catctttgta cacttctgca
     6541 agttgtgttt ctgtaaagaa ttcttcgtca gaaacagagt accagccttc atattcacct
     6601 aagtaaatat ctccctgttc taataaacga tcaaatattt tttgaacagc tgctttatgg
     6661 taatcatctg tcgtgcgaat gaatttatca taactgatat caagcgtttt ccataatttc
     6721 ttgacatcag ccgccatttt atctacatat tcttgcggtt ttacacctag ttcttctgct
     6781 tttgtttcga ttttttgacc atgttcatcc actccagtga gataaaacac atcaaagccc
     6841 atcaaccgtt tataacgagc aattgcgtca catgcaatcg tagtatacga gttgccgata
     6901 tgaagtttgc cgcttggata atagattggc gtagtgatat aaaaagtttc cttttctgcc
     6961 acggggctct tcctccttta tcgcatgatg cgaattcgtt tccttgctag tataccataa
     7021 aaaaagcatg ctgttagctt tttttcaaaa tgaaaaagac tggataaacg acttctatct
     7081 acgttcaatc tatcctttaa aaggatgaat aggttgaata aatgcgggtt tttgttttgt
     7141 ttcatatcta ctaaaaaacg gctttttatt tctcccctca cacgtggtat aataaagact
     7201 atctatctta gtaagggact ggaacaatca tgcagaagaa acatcttttt tttacactta
     7261 gtattgcttt tctttcatta gcacatttaa tattcagcta tttttatatt cgtatgtatg
     7321 gttattttaa cctccatggg catttgaaca gttttatgac agcagcttgg attttgcgtt
     7381 ttatcattga cgtatatatc gttatttgtg gcttctttgc cattcgtgaa gagcggtata
     7441 aagtattgcc gttttatctg cttttctttt tattcaatct catacttcca tttatttttc
     7501 atatttaaat gataacctag atttttcaag taaatccttg gttttttttc agagtctgct
     7561 acaataaggt aaaaagtact tttactgtca atggaggtgg tcagatgaac cgttttgctt
     7621 ctaaagaaga agaaaaatta tattatgaac ataaagcaag tgaagaagaa tttcttaact
     7681 ggtatcaaaa acaggaacgt ccagaatatg acaagccctc tttgactgtc gatatcgtac
     7741 tgatgtgcta taacaaagaa gaagatcagc tgaaagtttt gttgatccaa agaaaagggc
     7801 atccttatcg aaattcatgg gcattgcctg gtggctttgt ccaaaaggat gagtcaactg
     7861 gagaaagcgt cttaagagaa acaaaggaag aaactggtgt ggttatttca aaagaaaata
     7921 ttgagcaact gcatacattc agtacgccta atcgagatcc gcgaggctgg gtcgtcacag
     7981 tcagctactt agcctttatt ggagaagaac cgctaatcgc cggagatgat gccaaggaag
     8041 tacgctggtt cacactagaa cgtcatagta acaagatcca tttatctagc ggagaagtgg
     8101 ctatctcgct tgatttagtc actggagagt cttctggcaa agatactttg gcttttgacc
     8161 atagtcaaat catcttgaaa gcattcaacc gtgtagtcaa taaaatggaa cacgagccgc
     8221 aagttctaca agttttaggt aaagatttca cgattacgga agctcgaaaa gtatttgcta
     8281 aattcttagg gatcgattat aaaacgatcg atcattccaa ttttaagaaa gctatgctcc
     8341 aatatttcga tgagatcggt gaacgtcctg taggaatcgg ccgtccttca aaaatctatc
     8401 agctcaagcc ggggcaccat gaataaggtt gtgagcaaaa gattcagacc taatcattca
     8461 agaaaaaacg caaaaacaac tctttgtgag ttacgttttt tgtttcagaa ttttgatgta
     8521 ttcgtaaact ttctgaataa aaaattaaca atagcagaaa aagctcgtac gaactttttc
     8581 tgctattgtt ttattggttt gtcataatta gtgagctttt cttcaaaaga aaaattccta
     8641 gaaacaaaag tcattaagac tttttattaa caaggctaac cctaacgctc ctatcagttt
     8701 cattcatttt ttatcccttg ataaccgaat gtctgcccat tttttctact acttggctct
     8761 ttcttacgga attgctttct tctgctccgt cggtattgat caaaagaaat tttttattat
     8821 accattggga cttataacgc aattttgtta ttaatttatg ccagtcgtga atagaaaaat
     8881 cagcatgtgc ctcttctttt ggaaaagatt ctacaaataa gtagtcatat tgtttgacca
     8941 atttcccaga taattgttca agataatctt ctcgctggtt cttttgtttc agataaagat
     9001 ccattacttt tctcttttgt ttctgataat tgcttgcctg atctaacctg atctttctat
     9061 ggtgagcgga gcggactttc acattcaatt tccgctgtgc atgttgcagc ttttcttctg
     9121 tctggtgaaa ttttggtaaa gttacttcta gaggtcgaga tgtttggatc aatgtcttcg
     9181 gatgataggc gatcccgatc catttagcta aagattcttt ttttactggc gaaacatcac
     9241 aaagtagtgc gacataaaat tcttcattat atcgagcaga aatcgtagcg gagcgaatcg
     9301 atccttcaac tggccgatgc tgatttatct tgattagttc tttttgtttt ggtaacttta
     9361 agtagccatt ttccagatag atcgtacggg tttggttgtt ggtcgtataa gattgccaca
     9421 tacttttttt cgttttaagc tttgggaagc tggcacgtcc cttgaaataa ttgcgaaaag
     9481 cccgatccaa attaagctgt gcatttgcta aagcaagact gtctacttct tttaagaatg
     9541 ggtattcttt ttttaatgta gcgggtgtga gcgaaaaatt ggctactcct gtttgttgcc
     9601 aacttttctg ccgctttttc agcaaatggt tataggtaaa tcgagcacat ccaaaagtac
     9661 gaatcaattg ctgtttttgt tcttctgtcg gatacatacg gaatttaaaa gcttttagca
     9721 tctatgtccc tcctagttag caaccagaag agattccttt tgtcgtttta gtttttttgc
     9781 gtaatatggg acttcctgct cagttgcata gacaaaatgt tctggtgcga tttcacgaag
     9841 ttgacgtact tgtccgtcat ctggttcagg ttgatatttg attcttctgc gttttctctc
     9901 atggtctgga tctggtagag gaatagcaga taacaaactt tctgtataag gatgaacacc
     9961 gtgattatag atttcatcac ttgttcccat ctctaataac aagccgttat gcattacacc
    10021 gattcggtca ctaatatgtt taaccatcga caaatcatgt gcaataaata gataggtcaa
    10081 ttgatgttct ttttggagat cttgcaacaa attgaccact tgggcttgga tcgacacatc
    10141 tagtgcagaa attggttcat cacaaatgat aaaacgcggt tttactgcca atgctcgtgc
    10201 aataccaata cgctggcgtt gcccacctga aaattcatga ggataacgag taccgtggct
    10261 tggattcaat ccaacagttc tcaatagatc atcgactttt tttgcacgct cttcagatga
    10321 tttagctagt ccattaacat caattccttc ggcaatgata tctctgactt tcatacgagg
    10381 attcaacgaa gcgtatggat cttgaaagat catctgaaca tcatgccgga atttcgtcaa
    10441 acctttttta tctttgatct tcgttatatc ttgaccgtcg aataatatct caccatcagt
    10501 aggttcattc aagcgaatga tcgtacggcc agtagtggat tttccgcttc cagattctcc
    10561 aactaaaccg aatgtttctc cttcataaat gtgaaagctg atgtcattta cagcatgtac
    10621 ttcattcttt tttcctacat tgaaatactg tttcaatcct ttaacttcaa gtagtttatt
    10681 tggcatcagc aattcctcct tgaccgtaag tatgtttttg tttctcttta aagatagccc
    10741 agcgacgttg gatttcttct ggcggtgtca ctttgggtgc ctgcggagca agtagccaag
    10801 ttgctgcctt gtgtgttttt gatagttcaa aaaatggtgg ttcttgttct gcatcgattt
    10861 tcatcgcgaa ttcatttctt ggatagaatg cgtctccttt tggcgggttc aataaatccg
    10921 gtggagaacc aggaatagca tacaatcgat cgttcgcact atctaatgtc ggcattgaac
    10981 caagcagtcc ccaagtatag ggatgttgcg ggttgtagaa aatttcttcc gatgttccaa
    11041 cttcaacgat tcttcctcca tacatgacag cgacacgatc agcgacattc gctactacac
    11101 caaggtcatg agtgataaag ataattgatg tatcgatttt tgtctgaatt tctttcagca
    11161 gctccaaaat ctgtgcttga atagtcacat caagcgctgt tgtaggttca tcagcaatca
    11221 ggatttccgg ataacatacc agtgcaatcg cgatcacgat ccgttgacgc tgacctcctg
    11281 agaactgatg cggataactg ttgatacgtt tctcagcttc gggtattcca actaatttaa
    11341 gcaattctaa tgcagctttt tgtccctctt tttttgaaac tttattatgc tttctaaggg
    11401 actcagcgac ttgcttccca atgggcatcg tcggatctaa agaggtcatt ggatcttgga
    11461 aaatcatcgc tatttcttta ccacggattt tttgcatctc tctttctgat tttccaacga
    11521 tatcgttacc tttaaagagg atttgaccag catctatatt ggcgttgctt gacagcaagc
    11581 gcatgatgct tctggtcgtc actgattttc cacttccaga ttccccaacg atcgccaatg
    11641 tttctccttt attcaaatga aaattgacac cgcgaatcgc tcgtaccttt ccagcatacg
    11701 tatcaaaaga gatttctaag tctttcactt ctaaaatttt cgtcatcgtc ttcactcttt
    11761 cattttagga tcaaaggcat cacgcaatcc atcagcaagt aagttaaagc caatcatgat
    11821 aaccgataaa gttactgccg gaatccataa tagataaggc agatacagga atgtcttgta
    11881 cccttcgctc aacatcgttc ctaatgaagc tgacggaggc gtaagaccta aaccgataaa
    11941 gcttaaaaag gcttcgaaga aaatagcaga tggaatacta aacatcattt gaataataat
    12001 gacactagag atattcggca aaatatgttt gaatgcaatt ttcattcttg attcacctaa
    12061 tgtcaatcca gctaaaacat attcttgatc ttttaatttc attgtttgtg cacgaacgat
    12121 acgtgccatt ggtatccagt ttgtgatggc catagcagcc acgattgaaa aaattcctgg
    12181 ttcaaaaacg accaacatca aaatcatgac aacgaggttt ggaattcctg ataagatttc
    12241 caaaaaacgt tgcatcacgt tatcgacacg tccgcctaac aaaccagaaa ccaacccgta
    12301 agctacacca atcgttacat ctagtaatgc agcaataaat gcgatcagca acgagattct
    12361 cattcccata aataagcggc ttaatacatc acgtcccaaa ccgtctgttc ctaaaaagaa
    12421 atttacgttt gaagggacgt ttgcttgagc atatttatca acaagttcac caccaaccat
    12481 tgcttttcca ttcaatccat cgatattgat tcccggaata cgaggaggca aattgatata
    12541 agccacgttt tgggcagttg gatcatgagg agaaacaaag attgtgatca cagagatcaa
    12601 aatgatgacc aatagtaaaa ccattgaaat aacggcagct ttgtttttct ttaaacggcg
    12661 ccatgaatct tggataaaat tcaaagaagg tgcagtaatc tgttcacgtt cttcttcggt
    12721 atttttttgt aatggaagaa attcatccgc agggatatct gcaactgttt cataacgctt
    12781 tggtatcatt tccatttatc ctcggctccc ttctgaaaca cgaattcttg gatcaactag
    12841 accgtacaat agatccacga tcaaaataac aaaaatcagc ataaatgagt aaaggatcgt
    12901 tacagccatg atcgtaggat aatcatttgt tgtgattgac ttaacgaatt gttccccgat
    12961 accaggaata gcaaagatat tttctaccac cagtgatccg gtcatcaatg ctacagctaa
    13021 cggtcctaat aaagtcatca atggaatcaa actatttcga agaccatgtt taaaggcgat
    13081 ttcccaacgg ctcaatcctt ttgcttttgc taattccaca taatcactat gcagtacttc
    13141 caccatttcc gtacggataa accgagcaga gtcagcaagc ggcgacatgg ccaacgcaat
    13201 tgttggtaag attgtgtaca caaagccatc ccattttgcg attggcaaaa cttgtaattt
    13261 gattgcaaag atatattgta ataaaacagc aaatacaaag tttggaatgg aacgacctaa
    13321 tatcgctacg agtgtagaag atgtatctgc ccatgtattt tgcttcatgg cagagatcgt
    13381 tcctaaaatc gtccctagga ctgttccaaa gataattgcc tgtaacccta actgcaagga
    13441 tggtccgatt cttccagcta acaaatgtgc gactggctgg tttttaaatt ggaaggaaat
    13501 tccaaaatct ccttgaagca aattagaaag ataaatccca tattgtacga taactggttt
    13561 atccaaaccg acttgtttat ttagcatctc gattgtttca ggactcaaac gttcctgatt
    13621 cgtatatggc gtgcccggca gcaattgcat caagaaaaag gtgattgttg caattaacca
    13681 aagcgtgatg atcatgaaaa agacacgttt taatacatat ttgatataac tattcactaa
    13741 tgtttccccc tagcttttga cttctgacag atgtttcgct acattattag ataatgaacc
    13801 aaaaataaag gagcaatcaa atgaaaaaaa aatctatccc ctacgcagta gcttttctac
    13861 tgattctggt tattttaata aaaaatctca tcaaccattc ctttacactc attcaattat
    13921 ctaatgatct attcttatgg tcattgccgt ttctgattat cggcggtttt ttatgggtct
    13981 tttcttcggg tttcttcgat cattttcagc gatcggtcca tcttgcacga acaaggaacc
    14041 gaaagaaaaa accagaattt tcctcgctct cgtctgccag ctacggtatg tacagctttt
    14101 ggctgatcat tgccggaata ctaattgctc tttctgctat tttcatgctt ttttctttgt
    14161 taggataagt gaatatgaga acgaggttgc gacatttgcc gcagcctcgt tttttctcat
    14221 gctcacggta ttaacgatta ttcttcaacg tatgtccatt tgtaatccca tgaagcgcct
    14281 gccgcatggt gaacgatacc tttgattttt tcattcacta ggtgaccttc tgctttttgg
    14341 tacaccggaa cgacacccat atcatcagag atgatcttga ttgctttttg gaaatcatcc
    14401 caacgggctt gttcatcgcc agcgtctgtt gatgaagctg ctttaactgc ttcgtcatat
    14461 tctgcattgc tccagcgacc acggttgtat gaattacctg tgacaaacaa atcagtaaag
    14521 ctgcttgggt ctgcatagtc agcagcccat ccgcctagta ctgcgtcaaa atcacccgaa
    14581 gttgagcggt caagacgtac agagaatgga acaggtgttg gtttgactgt tacgccatcc
    14641 aagttttctt ggattgagtt ttgaacatat tcgattactt ttttcgatga atcatcatca
    14701 gaagccatca aatcaaattc taaagaatca acgcccaatt cttttttcgc tttttcccaa
    14761 tattcttttg ctttatcttt gtcataagaa actaattttc cagcttcttt tgcaaagtct
    14821 tcgttcgttg ttggactttt taccatatct gatgggatca atcctgtaga gacaactgat
    14881 ccgtcaccta gtacttgttt tactaaagct tcacgatcga tagaataaga aatagctttt
    14941 cttaggttca cattgttgaa tggtgaattc ttttcacgtt gattgaattc gatatagctt
    15001 gtccgcgctt ctttaacaga agtataagct ggatcgtttg cgttttgctg tgctaactca
    15061 ccagacaaga tcacgtcatc tgcttggcca tctttgaata agttcaatgc agtagaagat
    15121 tcttttacta cactgacttt gatttcagaa agttttactg tatctttgtc ccagtaatga
    15181 tcatttttct tataagtcca ttctgtatct gttcctggtc catcaaaatc ttctaatgtg
    15241 aatggtccat tataaacagc tttatcactt gcagatgcat aggcatcccc attttcttcc
    15301 actacggatt gtttttgtgg gaaaaatgat gggaaagcaa gcagatattg gaaatatggt
    15361 gtttgtttcg tcaatttgat ttctaattca tagtcaccaa ctgctttgat ccctagttct
    15421 gatttatctt tctttccagc tgtaatgtct tctgcatttt caactggcgc atacaggtaa
    15481 gcatattcag aagctgtagc tgggtctact gttcgttgcc agccataaac gtaatccgct
    15541 gctgtaacag gatcaccatt tgaccatttt gcgtcttctc gtaatttgat tttgtaagtt
    15601 aatccgtctt cactgacttc tgccaactcg ctagctcctg ccggctgtgg tttgctgtct
    15661 tcatcaagac ggtaaattcc ttcataaacg ttattcaatg ctgtaaaact gattgtatct
    15721 gttgcaactg ataagtcagc tgtaggcatt tcttgaggca cgactaagtt aaatacgcct
    15781 cctccatctg ctgaaccgct ttttgctgaa ctactgcttg tggctgaact gccgccataa
    15841 catccagcaa gtacaaggcc gcataccgca accaaaccaa atgtaaattt ctttttcatt
    15901 ttacgtaccc ctttattgga attttcaga
//
LOCUS       DACENJ010000049        15485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.049, whole genome shotgun sequence.
ACCESSION   DACENJ010000049 DACENJ010000000
VERSION     DACENJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.049"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..698
                     /locus_tag="IW748_001120"
     CDS             <1..698
                     /locus_tag="IW748_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1104040.1"
                     /translation="FNSSIEVPYIEKVSADVLKVEEITYENILKDINFDVKVGDVIGL
                     VGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELAL
                     GNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTS
                     GLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVED
                     STLIEQIFNDITK"
     gene            716..1285
                     /locus_tag="IW748_001121"
     CDS             716..1285
                     /locus_tag="IW748_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1104041.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            1395..3149
                     /locus_tag="IW748_001122"
     CDS             1395..3149
                     /locus_tag="IW748_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104042.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            3103..4875
                     /locus_tag="IW748_001123"
     CDS             3103..4875
                     /locus_tag="IW748_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104043.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            5398..5667
                     /gene="rpsN"
                     /locus_tag="IW748_001124"
     CDS             5398..5667
                     /gene="rpsN"
                     /locus_tag="IW748_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1104044.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            5682..5861
                     /gene="rpmF"
                     /locus_tag="IW748_001125"
     CDS             5682..5861
                     /gene="rpmF"
                     /locus_tag="IW748_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1104045.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            5893..6270
                     /locus_tag="IW748_001126"
     CDS             5893..6270
                     /locus_tag="IW748_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104046.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            6291..6440
                     /gene="rpmG"
                     /locus_tag="IW748_001127"
     CDS             6291..6440
                     /gene="rpmG"
                     /locus_tag="IW748_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1104047.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            6464..7447
                     /locus_tag="IW748_001128"
     CDS             6464..7447
                     /locus_tag="IW748_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1104048.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            7477..7599
                     /locus_tag="IW748_001129"
     CDS             7477..7599
                     /locus_tag="IW748_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1104049.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            7617..9140
                     /locus_tag="IW748_001130"
     CDS             7617..9140
                     /locus_tag="IW748_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1104050.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            9164..9403
                     /locus_tag="IW748_001131"
     CDS             9164..9403
                     /locus_tag="IW748_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104051.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(9596..10057)
                     /locus_tag="IW748_001132"
     CDS             complement(9596..10057)
                     /locus_tag="IW748_001132"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1104052.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(10252..10470)
                     /locus_tag="IW748_001133"
     CDS             complement(10252..10470)
                     /locus_tag="IW748_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1104053.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            10588..12204
                     /locus_tag="IW748_001134"
     CDS             10588..12204
                     /locus_tag="IW748_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1104054.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(12445..13785)
                     /locus_tag="IW748_001135"
                     /pseudo
     CDS             complement(12445..13785)
                     /locus_tag="IW748_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14061..14216)
                     /locus_tag="IW748_001136"
     CDS             complement(14061..14216)
                     /locus_tag="IW748_001136"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1104055.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(14512..14844)
                     /locus_tag="IW748_001137"
     CDS             complement(14512..14844)
                     /locus_tag="IW748_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1104056.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(15358..>15485)
                     /locus_tag="IW748_001138"
     CDS             complement(15358..>15485)
                     /locus_tag="IW748_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1104057.1"
                     /translation="LKRFSSLIHKQSWRTGKYDQDCYQECMLAIYLAISKFEIKE"
BASE COUNT         5208 a         2326 c         2998 g         4953 t
ORIGIN      
        1 tttttaatag ttcgattgaa gttccctata tcgaaaaggt aagtgcagat gtattaaaag
       61 ttgaagaaat tacttatgaa aatattctaa aagacataaa ttttgatgtc aaagttggag
      121 atgttattgg tttggtaggg aaaaacggtg tggggaaaac aaccttacta agaatactca
      181 tgaagattat tcgccctacc aaaggaaaga taatcgaaaa taaaaaaaat ttgtcatcgc
      241 cgtttttagt aatgcaagaa atggattacc aatttttcac agaatcagtg aggagcgagt
      301 tagccctggg gaatgaaata gttagtaaag atcagcaaga aaaaatttta aaaaaaatgg
      361 aactttataa aatgaaagat caattaccgt ttgatttatc cggtggagaa aaacaaagat
      421 tattggtttc tatagcttca ttgtcaaaaa ctaatttatt tctatttgat gaacctacaa
      481 gtgggttgga ttatatcaat atggaaagga ttgctgacct tattaaagac ttaaaagaaa
      541 agggtgcagt agttatagcc actcatgatc cggaatttct ttataaaaca tgcaatagaa
      601 ttatttattt aaaaaatggt ggaataaaaa aagatataaa acttagagta gaagattcaa
      661 ctttgataga acagattttt aatgacataa cgaaatagaa ttgaggagaa tatatatgaa
      721 aacaaaagat tatattttta ttggtttagc tacagttatt tccctagtaa tttacttttt
      781 ttcaataatg gttagtagta ttggtggggc gtttggtcac agcatatccc cagggatttt
      841 tggattatta tctggaatca tatttgttta tattagctat aactacccta gaaaaggaat
      901 ttttactatt tacacaattg ttttattgtt attttttacg ttaatgggcg gggcgtactt
      961 accttggttt atcagttcga ttagtatggc gatcttagct gatattattc tatctgtatt
     1021 tggatatgat cgagcaattc ctcaagttgt gtcttgggtt ctaatgcaac taggtagtgc
     1081 tgccggacag tggattccca tatggttctt tacagatcgt tttcgtcaag actggataga
     1141 tagaggacaa tctgctgcaa caatggatgc aatgatccac tacgcggtgg gtatatgggg
     1201 aattatttca gtgttagttg tagcaagttt atctatgatt ggtgtattga ttggaagaaa
     1261 agtcttaaaa aaatacaaaa aatagaacgt agtttacctt ctatctgttt gtacatctct
     1321 aatttacgct attacggaaa gcattaacag ttgtaaacga ataagagaag aaattgaatg
     1381 cgggggagga aaaaatgaaa gtatacaaaa aattatttag ttatgtccct aaaatgaaat
     1441 actacggatg gttagccact ttattttctg atttttctgt cgtattaaca gtctatggat
     1501 attacagtat ttacaaattt cttcagtctc taattattgt aggagatgaa tatttagcta
     1561 agagttatgc ggttcaaact gttgcttggt taactttagg agctttattt tatatttttt
     1621 ctggcttatt ttctcatatc cttgggtttc gcttagagac taatttgaga aaaaagggaa
     1681 ttagcggttt aacagaagca agttttcgtt tttttgattt aaattcttcg ggatatattc
     1741 gaaagacaat tgatgacaac gcggctaaaa cacatatggc aattgctcac ttgattccag
     1801 atagtgctca agccatagta acacctgttt tatcagttgc cttaggcttt ttcattaact
     1861 ttaaaattgg attggttcta atcgccatgt tagtaattgg tgtattcttg ttaaagaaaa
     1921 tgacagagaa tacaaatttt attcaaaaat atcaagaatc gttagatgtg ttaagctctg
     1981 aaacggtaga atatatccgc ggaattcaag ttataaagat atttggtgct aaagtaacat
     2041 catttaaagc cttgaataaa gcaattaacg attatgctaa gtttgcatat aagtattctt
     2101 tgagtgggaa aacacctttt gttctttttc aatggctatt ttttggcata gtttgcattt
     2161 tgatagttcc tataaccttt tttataactt ctcttaccac tcccaagatc ttagctgttg
     2221 acttgttgat gttgtttttt ttaagtggaa ttatttttgt ttctttcatg agaataatgt
     2281 atgtaagtat gcacttgttt gaagcaagat atgcagtcga aacactagac tccctttatt
     2341 tagaaatgaa gcaagaccaa ctaaaatatg ggaacgagac acagattaaa aattgtgaca
     2401 ttgcatttga aaatgtcagc ttttcataca aagaagagtg tgttttagaa aatttcaact
     2461 tagttttaga acaaaaaaga atttatgcat tagtaggaaa atcaggaagc ggaaagagta
     2521 cgatagctaa actaatttca ggtttctaca atgtggataa gggacagatt aaaattggtg
     2581 gtaagccttt aaatcactat tcaaaagaca caattattaa cacaattgct tttgtctttc
     2641 aagatacgaa actattcaat cagagtattt acgataacgt ttctatagca aatcctcaag
     2701 cgccagcaag taaagtattt gacgcgctaa gattagccgg ttgcgaatcg attttagaaa
     2761 aatttcccga aagagagcat accttaattg gtagtaaagg agtttatctg tcaggaggag
     2821 aaaaacaacg tatagctatt gccagagcaa ttttaaaaga tgcaaagatt gttattatgg
     2881 atgaagccag tgcatctatt gatccagata atgagtatga actacaaaag gcttttcaat
     2941 atttgatgaa agaaaaaaca gttattatga tagctcatcg attatccagc attaaatctg
     3001 tagatgaaat tcttgtcttg tcagaaggaa aaatagtaga acgtggaaat cataatgaat
     3061 taatggctaa acatcgagtg tataaacaat tggtcactaa ttatgagaat gcaaatgatt
     3121 ggaggcttga caatgaaaaa tttttataaa aaaaaatttg ctttaactga tcagggcgca
     3181 gaagctttaa ctaaagcttc tatttctagt ttttttgtat actgcattaa tatggtacca
     3241 gcattcatta taatgatgct gatagacgaa ttagttttag aaaatgcaaa gccccgttgg
     3301 ctttattttg cagtatcgtt tgttactttg cttttcatgt attggttgtt agatagggag
     3361 tatgaaaact tatataatag tacttataaa gaaagtgcgc atttaagagt gcaaattgca
     3421 gacgatttgt caaatttacc attatcctat ttttcaaaac ataatttatc agatttatct
     3481 caaactatca tgtctgacgt tgaaggtatt gagcatgcga tgagtcatgc aatacctaaa
     3541 gccggcggta tggctctgtt tttccctttt atttcagtga tgcttttggt tggtaatgtc
     3601 aaaatgggat tagctgttat tttgccaacg ttatttagtt ttgtcttaat cttgttatca
     3661 aagaaatccc aaacgaaagc caatactaaa tattacgata ctttgagaga aaactcggaa
     3721 gaatttcaag aaactattga attgcagcaa gagattaata gctttaatct atctaaaaaa
     3781 gttcaagaca gacttttcaa aaaaatggaa gagagtgaaa ggattcattt aaaggtagaa
     3841 ttaagtactt tttcagtcat ggccttatcc tctattttct catatgttag tttagcagta
     3901 gtcattctag taggtgttca cttactgtta acgggtgaag tgactatact ctacgtcgtt
     3961 ggttacttac tagccgcaat aaaaataaag gattccttcg attctatgaa agaagcagtg
     4021 cttgaaatat tttatttagc tcctaaaata cagcgaatta gagctatgaa ggaaaccagt
     4081 atccaagagg gatcagatag tcctctgaaa tcttttgaca ttgagttgcg agatgtctct
     4141 ttctcatatg ataacaatac cccaatttta gatcatatct cttttacggc taaacaagga
     4201 gaagtgactg cgttagtagg tgctagtggt tccgggaaaa ctagtatctt gaaattagtg
     4261 tctagactgt atgattatga tgaaggctgt attttaattg atgggtatga tattaaacgt
     4321 gtatctccag cttctttgtt tagcaaaatc gctattgttt ttcaagaggt aactttgttt
     4381 aatacaagta ttttggaaaa tatacgtatt ggaaatagtc aagcgtcaga tgaggaagtg
     4441 aaaaaagcag ctcgtttagc taactgcgaa gactttattg aaaaactacc tgacggttat
     4501 catacattgg tcggagaaaa cggaagttca ctttctggag gagagcgaca acgcctatcc
     4561 atagcacggg cttttttgaa aaatgcgcca attttaattt tagatgaaat tactgccagt
     4621 ttagatgcgg aaaatgaaaa acgtattcag gaaagtttaa atcgtttaat acaagataag
     4681 acagtactaa ttatatcgca tcgcttgaag tctattgaaa aggtaaataa aattgtagtg
     4741 atggatcaag gaaaactagt tgatcaaggt actcataatg agttgtatcg aaggtcagaa
     4801 atatacaaaa atctcataaa gaaaactaaa ctatctgaga aattcgtcta tgaaaaagaa
     4861 gctcaatcac gatgatgaaa taggctttaa attaacctac aatttttggg atatagttat
     4921 aattcttttt caaaaagaat aattttatta aacagcatct aattttgaat acacgaaaga
     4981 atcagtatga ttgaattagt tcacttaatg tctattctta taatataagg aataatcaaa
     5041 gttttaaaaa attggcgata aaccagttaa acataaaaga ttgaataaaa agttatagtt
     5101 tctttcatat tgccgtttta agtagaggaa aagctactta aaacggtatt tgtgtttgat
     5161 tggactaata aaagtgaatt cttatttttc agagcctaaa ctgcatattc ttttaaatga
     5221 tttgattatc tctatgtacc gataaattcg ttttcaaata gttatctatt catatgagtt
     5281 ttatttcatg ttatttattg ttgacaagag caactcttgt tggtaaagta tcagtgtcaa
     5341 aacgtaacga ttacgatttg aggtgtctaa atcaaaataa aaggagtata aaggtatatg
     5401 gcaaaaaagt cgaaaattgc taaagcaaag aaacaaatgg cgatgattga aaagtatgca
     5461 gataaacgtc aggaattaaa agccgcagga gatcgcaccg ctctcgctaa attgccaaga
     5521 gattcaaatc cgaatcgtct gaggcttcgt gatcagacgg atggtcgtcc aagagggtat
     5581 atgcgaaagt tcggtatgtc ccgtattaag tttagagaac ttgcccatca agggttgatt
     5641 cctggagtaa aaaaagcaag ttggtaaaag gaggaaatat catggcagta ccagcaagaa
     5701 aaacatctaa ggcgaagaaa cggttaagaa gaactcatca aactgtagta aaaccagaaa
     5761 tttcatttga cgaagcgaat ggtgattata gacgtagcca tcatgtatct ttaaaagggt
     5821 actataatgg aaagaaagta attaaaggag cttcaaaata gtttaatata taacttttag
     5881 gagggaatca aaatggaaat tatttaccca cctttagttg aagaaggatt aaagtattat
     5941 cttgaaacga cccagcaatc gctagataaa tgcacttttt atcgttcgat ggtagagaga
     6001 ggaattatta ctgaaacggg tttaccgacc caacaggcaa ttgaaaatgg attagtcaaa
     6061 gattactatg aggaccaagg cttatcattt gatgattttt taagaatcta tccaattttt
     6121 gaagagtatg acgaggagtt atttcaatgt atcgatggtt attgggagat acctgttgat
     6181 atgaaagaaa atttagtttc acaattggaa tctggcgagt taacttttga agatgcacaa
     6241 caaattcaag catatcttga agatcgataa gtattgaagg aggaagattt atgcgtcaaa
     6301 atattatttt ggaatgtgtc gaaactggag aacgtctgta tctcaccagc aaaaataaac
     6361 ggaataaccc tgaacggtta gaattaaaaa agtattcgcc aaaattgcgc agacgggcaa
     6421 tttttaaaga agtgaaatag ctaagtggta aaaaggtgac aaaatgggca taccagttac
     6481 aatcgtttct gggtttttag gggctggaaa aacaacgcta atcaatcaag ccttgcaagt
     6541 ctcaccctac cccagggaag aaattattat aattgaaaat gagtttggtg aagttgggat
     6601 tgatcacaaa ttattagttc atagcgagga acgggtgtta caactaaata atggatgtat
     6661 gtgttgcagt cttcgcagtg atttattagc aatattgatt tctttactgg aaacaataga
     6721 agaacatcaa caaccaattt cacaaatcat cattgaaact acaggtatag cagatcccca
     6781 accaatcatt caaacacttt taacagcgcc tcaactaaga ggaagagtat atatcgatag
     6841 tttattgact gttattgata gtgacagttt tgaacttatt gaaagagaac cacaagccct
     6901 taagcaactt gtcatggcag atcgcatttt tgtgtctaga aaacaaaact tgaatttatc
     6961 tgcactaaaa aaattacaga gagaaatcaa aagtattaac cctttatccg atattcgaat
     7021 gttcacacat agtgaaacga ttcaaaaaaa ggatttttat aatttgaaaa aatttaatca
     7081 tcccttagtt tttgaaagtg attcatcaga aaatcactat aatggcaaat actatcatca
     7141 acaccatcaa gtacatgagt ttaaatcaat tctcttaact tcagatcaag acataaaaaa
     7201 agatttttta ctccaatgga ttgactggct gattttaacc aaccaaggga gcctgtatcg
     7261 ttttaaagga ttgattgctt tacaagagca ggattttata gttgccatgc aagaggtaaa
     7321 cgaacaggtt aattttcaat acaccactcg acaaaaagac caaaccacta ttattctaat
     7381 cggaaaaatg ttggatgaag aaaggatcaa acagagtttt gataaattac tggcggaaag
     7441 caaataatga taagtagtga ttgggggtaa tttctaatgg agaaaacgga tatatctagt
     7501 gcatatcgac gacttaaaag cccgaatatt aaaactagaa aaagggcatt aaaaattatc
     7561 aaagaacata aacgaaataa aatgaagaaa ttagcataat taaggaagga aaaagaatga
     7621 agaaaatcag tgtaggactt attgggatag cagctctagg attattggga gcttgctcca
     7681 gcactaatga tgcaaaagta tcgaatgata aagatggaaa attagaaatt gtaacaacat
     7741 tctatccaat gtatgatttc accaaaaaca tcgtcggcga tgaagcgaac gtggatttaa
     7801 tggttccagc gggatcagaa cctcatgact atgagccctc agctaaggat atggcaaaag
     7861 ctcatgatgc tgatgttttt gtatatcata acgaaaacat ggaatcatgg gtaccaaaag
     7921 ctaaagaaag ttggaagaaa gctgggccta atgtagtaga aggaacaaaa gacatgattt
     7981 tattgccagg tagtgaagaa gaagatcatg atcacggaga agaagatcac catcatgaac
     8041 tagatcctca tacgtgggtt tcacccaaaa tggcaattaa ggaagtttca aatatcaagg
     8101 atcagttagt taaattgtat ccgaaaaaag caaaagtatt tgagacaaat gctgaaaagt
     8161 atttgactaa actgaaacgg ttggatgctg actataccac tagtcttaaa gaagcgaaac
     8221 aaaaaagttt tgtgacgcaa cacgcagcct ttggatattt agctttggat tatggattga
     8281 aacaagttcc tattgctgga ctaagtcctg aagaagagcc atcttctggt cgattagctg
     8341 aattaaaaga gtatgttaaa aaaaataaga tcaattatat ttactttgaa aagaatgcta
     8401 atgataaaat tgcaaaaact ttggccaacg aagctgggat aaaattggaa gtactgaatc
     8461 ctcttgaaag tcttacaaaa gaacaaatgg ataatggtga agattatgtt tcagtaatgg
     8521 aagacaatct aaaagcgtta gaaaaaacaa ctatggttgc tggtgaagag gtagttccag
     8581 aaaaagaagc gaaggatgaa aagacagttg ctaatgggta ctttaaagat gccgatgtga
     8641 aggatcgcga attgagtgat tatacaggcg aatggcaatc tgtttatccg ttgttaaaag
     8701 atggaatttt ggatgaagtt ttcgattaca aagcaaaaat caataaagac atgacagctg
     8761 ccgagtacaa agattattat actactggct ataagacaga tattgatact atcaacatta
     8821 aagacaacac cattgatttt gtagtcaacg gtgaacatca tcaatacaca tacaaatatg
     8881 ttggatacaa aattttgaat tatgaaaaag gaaaccgtgg tgttcgtttt aattttgaaa
     8941 ctgatgatgc aggtgctggt cgttttaaat atattcaatt tagtgatcat ggaattgcac
     9001 caagtaaggc agagcatttc catattttct ttggtggaga aagtcaagaa aaactctata
     9061 atgaattgca taattggcct actttctatc cagcttcatt gtctaaacat gaaattgctc
     9121 aagaaatgat ggctcattaa aattcaattt ttggaggaaa tacatggtaa gaaaagtatt
     9181 attgtctatg gattccaaga atattgatgc ttatcggcgt tatcgatttg caaagcaaat
     9241 gagattaaat gggagtacat tagctgatat tgctgcaact ttacatgtta gtattgacac
     9301 agttcataga gtattaaaac gggatcctga ttgttgtttt atcaaggtca atactggaag
     9361 aagtagacgt ttagcaaaac ggcataatag cttggccaat taattgataa taccagagta
     9421 gatcattttt cccttatttg gatttgatct gctttttatt atgtcttgaa ttgtcaaatc
     9481 aagcgcaaca ctttatcaaa gaagacttca taaggtgttc tccaatctaa acactttcta
     9541 ggtctgttat tcagtttttc aatcatctga tttatcgttg agttatctac cgtagctatc
     9601 tctttcgatt ttggcaagta ttctcgaatg agtctattcg tattttaatt tgttcattgt
     9661 tgccaagggc atgaggatct ggaaaataaa agggcatatt attcaatgct tctgtaactg
     9721 cgaaatgatt ggagaattct tttcctctat ctggagtgat cgttttgacc gcctcatttg
     9781 gaatactgct taaaagttca atcattttct cgctcacata agcggctttc tttctgggga
     9841 ccttctttcc tgctaaaagg aagcatgatt ttctgtctgt caaagtcact aaacaagtac
     9901 caccagtttt gccagctaca atatcggctt tccaatgacc aaaatcactt ctatcatctg
     9961 cggattttgg acggccatgg atcgaatgag taaggcaaat ctttccacga gactcgacat
    10021 gattttttgt atgtcttgtt tttcctcgat ggtgcaatga acggatcagg cctttattgc
    10081 cgtgtgagag gggttctgta tcgaaaagat cattgcaaat tgcacgttag atcgtgataa
    10141 aactaattga aaagtccata tgttcatgct tcaaacgatt cgagatttcc tctggcgacc
    10201 actgaaaatc gacaaataaa tgacgaacaa ttgaccaaac tcacggatca tttagaatgt
    10261 gataacgtcc gcattttttt cttcgataag cataattttt ttccgctttg gaaggtgaat
    10321 agtttttttc ttgtttcttc tcagttcatg taaaatagta aacgggctat gctgaatgtc
    10381 gttgccaatt atactaatag aaaatccttc ttcgtgaagc agaaagattc tttctcgttc
    10441 gagtatagta agatgacgat actggctcat aattattttc cttctaggag tgttctcgac
    10501 aaacttatac tagttggata tcgattatga ctcattttta tgtgttgcac ttaaagtgta
    10561 aattcaaggt atagaaagag gttccctatg aaaaaaatac ctattacaat cttaactggc
    10621 tatcttggtt caggaaaaac gatacttcta aatcatattc tgtacggtga tcacggaaag
    10681 gaaattgctg tcattgtaaa tgacataggg gaagtaaatg ttgatggggt attagtcgac
    10741 aagaaaggtt ttaaaagaac tggagaaaaa cttgtttcga tgccgaatgg atgcatttgt
    10801 tgtacattac gcgaagattt gattgttgaa ctagatcaat tagtgcaatt ttctgacttg
    10861 aatcaaattg ttattgaagc ttcaggaatc agcaaaccaa ttccaattat tcaagcaatt
    10921 atattagcta aaaacccttt agacattgat ttgacccaac gattagagat tggtgccatt
    10981 attatagtag tggatgtaaa acgtttttgg gataatttcg aaacgggtga aaagattcag
    11041 gaaagaaaag ctgaaggtaa tgtagttgaa gaagaacagg atattcgtgg cttgttaatt
    11101 gatcaattcg agtgttgtaa catattactt ttaaataata gtgacttagt gtcaaccaag
    11161 acgatttaca aaattaacgg tctaattgct aagttacagt ccaatgccaa aataattagg
    11221 acaattaaag gtgaagtaga cttatcgaaa attctaaata caaatctttt taatatagat
    11281 gagacttcct attcagtggg ctggttcaaa gagttagagc ttggctatca aaatcatatt
    11341 cctgaaacag aagaatatgg tatctcaagt tttatctatc gaaatcggtt actccttgac
    11401 gcacaaaaat ttgcccatct tacggataaa aattttcctg gcaatattac aagagcaaaa
    11461 gggtttcttt ggtttgccca acatagtgat ttttcgacga tctttgaaac atcgggtagc
    11521 tataaaagta ttgaatcatt aaattattgg gtagctagtt tacctaaaat tgagtgtcaa
    11581 atgattatac aaaaaaatcc cgaattgaag gaagcctggc atccgttgtt tggtgaacgc
    11641 attaataaac tagtttttat tggtatgaag atggatcaac aatgtattaa ggaacaactt
    11701 gagggttgtt taactgattc tcttaatgca gataatttcc gaaaaatttc accatttcca
    11761 tggaaaaagt ctaatatgtt tagccagata tcgttaatga aatttttaag aaaagctggt
    11821 gtcaagatag gcgatgtcat aattattggc attttgatta ttacctcttt tattccctta
    11881 tttgtattct ctatagaaaa ggagaatgaa acaactgaag atgctatcaa atatgccatc
    11941 gttcgaattg atggcaagga aatggatcgc ttcgatttgg ataagatcaa tcataaactc
    12001 gtgaattatc atcctgcgaa gggacaatat aatattgttg agattaaaga tggaagaatt
    12061 cgagttaaag aagataatag tcctgatcaa atcgcggtta gaactggatg gatctcaaaa
    12121 cccggtcaaa cgagtatttg tttaccccat aagttagtta ttagtattga gaaaaaagaa
    12181 agcacagatt actacattta ctaatctgga aatagatgag gaggttttta ataagaaaaa
    12241 gatattatta ttaataatag caggggcttt attaagtgtg ttaagcgctt gtggacaaga
    12301 aaaagaagcg gtgacaacag gcacaagtat ctttaacggg acctgcaatg gtttagttaa
    12361 cagtcggtta gattacttat tttggctctt cgtcaatgag tgttggtgaa gaaattcaat
    12421 agaataatcg agatactcac acggctagac agctatcttg tctagccgtg ttgttgttct
    12481 ggctttagtc agttgggtag tattcttagt tttcttaaaa tattttcctc gatataaaaa
    12541 gattcgttct cggtagtttt gaaagtttct atagccatag gcaattcgtt ttagtacttt
    12601 gatatggtta ttcaaacatt ctatagggcc gttggaatag ggaacgcgca acgctcgttt
    12661 gatttctgtt tggtatttct tgaaggtcgt gaacactggt tggtagtgct ctggcaactc
    12721 cttgtagtct tcttttagga gcgcgacgaa ttcaggaaca tcttgtcttc tgataacgga
    12781 aagaaaatcc tggtagactt cgtagcctcg ctttaaggaa tcatcataag ataatagccg
    12841 atcgactatc tctgcttctg tcagatgatc tcgaaatgac ggacgccagt agcgatgctg
    12901 acctttcaat tcccacgcat tcttttgaag tagtttccag tatttcttta agtgcttcga
    12961 ccgacgatcc ccttttttaa aggtcttcat tacttgaatt cggtggttgg taaaagcccg
    13021 accaatgtgt tggctaatat gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc
    13081 tttcaccaat gtgcgatagg gtgtatagaa atctgaaacc actagacgca cttgttcacg
    13141 gacttttata gggtagcgca agaaatagtc gcgtaaagga ttgagttgcc gattttccac
    13201 aatatcaatc agttgatggg tcttgccgtc catcatgaca aagctcatgg ctcccgagac
    13261 atttttcact gatttgaatt catcgaaaca caccacttct ggtaacagct ggctcaaaga
    13321 aatggtttta ggccgaaggc tgcgaagtat tcgaatgaca gtggtcggag aaatgtgctt
    13381 catacgtgcg atcaaagaca tagaaagagg ttcgcttaat agctcaagaa tggcttgttt
    13441 gactctgcga gcaatggagc agcgacgatc actaactgaa tcttctgcta aaaaagtctt
    13501 tccacagtgt ttacatttga agcgctgttt gttaatccgc agaatggtct tgtagttact
    13561 gacatcattc aataagatgg atactttttt ccagccctat ttaatgatcg tttgttcatt
    13621 ttgatagtgg cgatgaaagc attcctccgg ttgataggtc aatgtgccat caaaagcaaa
    13681 acatgtttcg tcattgatgc gctctttgcg aaaggcatcc tcatgaaagg taagatttag
    13741 gtctagaata tctagtaact ctttggtaaa ctctgaatag gacatcttga aacactactt
    13801 tgaatggttt ttgtcgactt tattctaaag gattttaagg tgtctgtttg cgtttattag
    13861 aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca ctagatatta tagatccctt
    13921 attttcttcg cttcaaattg tcaaggagtc atcataaatt ttgtatggat tcttttgttt
    13981 gggtagtagt cttctaaata atcttggatt aacaccttca tctctatttt acttttatat
    14041 ttgttggagt cgattaattc tcatttgatt gatcgatgaa acgcttcaat tactgcattg
    14101 tcaacaggag tcccagtttg gctcactgaa tgtgtgatgt ggtggtcgtt caacagctct
    14161 tgataaataa aagaccgatg ctggctacct ctatcggaat gaattaccgt aggcaattct
    14221 ccttaataaa tttgtaatgg acggacgaca atctcttttt ttatgtgtga atctaccata
    14281 tagtaacgta gaatacgagt tgctaagtca atataggtgg acaaatagag gcgaccgtca
    14341 ttacatggaa tgtaagtaat atccatcacc caaacagcat caatttggtt ctgttcaaat
    14401 tatcgattca acattttttt accatttctt tgattgtttt ttctctacca tgagaagatt
    14461 tgcgtcgatg aaatcctctt gtgcataaat tcatttcacg cataagaaca gctacaacac
    14521 gtttattgat ttcgattcct tcatcgtaaa gtttttgagt gatccctgga gatccgtatc
    14581 gatctttgtg ttcgtagaac actcgtcttc ctcttttagt cgtttatttt cctgttcaag
    14641 aatgtagaaa tctgctttat caggtaatct ttttccttgt ccagcaaaag catttttccc
    14701 gtatcgacga tattctgaaa cccatcggga aattgttgga cgagagatat tgtattcttc
    14761 agcaattttc ttggtagtta cttttttctc aaggatatcc tgtgaaatct ttagtttaaa
    14821 tttacgatca tatgtttttc tcaatagagt tcattccttc cggtaacgta actgatagtc
    14881 aagaaaagtg tatcatagcc caacttagta tcagggtttg tcaactaaac tgtggaagtt
    14941 gatagataag agtttttaac aaccaaaatt ctcttgacta tttttgaaat cagacaaatt
    15001 ttgattggac acatagttga ataaatctac cgtcgttacg gaaggtaaat cgcatgcttt
    15061 tcaattctag aggagaagtc ctcgttgaag taccatagcc cagttctgga ttcgacctcc
    15121 tgcccacttc atacgtaaat aaagtaattt taggagtgag actgtgacaa aagttatttt
    15181 taagctaaaa acgaattaaa aaacgttgat aattaaaatc attcgtttta gattctatga
    15241 atcattatag aacgctgtaa aatcgatact ttataattat aaagaagata gtttcaaatt
    15301 tattgttcga aatttgaaac tatcttttta cttatgtcac agactccctt agttgagtta
    15361 ttctttaatt tcgaatttcg agatagctag atatattgca agcatacatt cttgataaca
    15421 atcttgatca tattttccag ttctccagga ttgcttatga atcaaagagc taaatctttt
    15481 gagaa
//
LOCUS       DACENJ010000050        15387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.050, whole genome shotgun sequence.
ACCESSION   DACENJ010000050 DACENJ010000000
VERSION     DACENJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.050"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..230)
                     /locus_tag="IW748_001139"
     CDS             complement(<1..230)
                     /locus_tag="IW748_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1104058.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKI"
     gene            complement(243..3002)
                     /locus_tag="IW748_001140"
     CDS             complement(243..3002)
                     /locus_tag="IW748_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1104059.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(2999..3703)
                     /locus_tag="IW748_001141"
     CDS             complement(2999..3703)
                     /locus_tag="IW748_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1104060.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(3715..6024)
                     /locus_tag="IW748_001142"
     CDS             complement(3715..6024)
                     /locus_tag="IW748_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1104061.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(6219..6683)
                     /locus_tag="IW748_001143"
     CDS             complement(6219..6683)
                     /locus_tag="IW748_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104062.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(6683..7309)
                     /locus_tag="IW748_001144"
     CDS             complement(6683..7309)
                     /locus_tag="IW748_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1104063.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(7316..7681)
                     /locus_tag="IW748_001145"
     CDS             complement(7316..7681)
                     /locus_tag="IW748_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1104064.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(7671..8009)
                     /locus_tag="IW748_001146"
     CDS             complement(7671..8009)
                     /locus_tag="IW748_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104065.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(7999..8325)
                     /locus_tag="IW748_001147"
     CDS             complement(7999..8325)
                     /locus_tag="IW748_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1104066.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(8303..8584)
                     /locus_tag="IW748_001148"
     CDS             complement(8303..8584)
                     /locus_tag="IW748_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1104067.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(8586..9950)
                     /locus_tag="IW748_001149"
     CDS             complement(8586..9950)
                     /locus_tag="IW748_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1104068.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(9963..10538)
                     /locus_tag="IW748_001150"
     CDS             complement(9963..10538)
                     /locus_tag="IW748_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAQ1104069.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(10504..11691)
                     /locus_tag="IW748_001151"
     CDS             complement(10504..11691)
                     /locus_tag="IW748_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1104070.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(11755..13482)
                     /locus_tag="IW748_001152"
     CDS             complement(11755..13482)
                     /locus_tag="IW748_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1104071.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(13479..13931)
                     /locus_tag="IW748_001153"
     CDS             complement(13479..13931)
                     /locus_tag="IW748_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAQ1104072.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(14043..14423)
                     /locus_tag="IW748_001154"
     CDS             complement(14043..14423)
                     /locus_tag="IW748_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1104073.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(14420..14806)
                     /locus_tag="IW748_001155"
     CDS             complement(14420..14806)
                     /locus_tag="IW748_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104074.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(14808..15086)
                     /locus_tag="IW748_001156"
     CDS             complement(14808..15086)
                     /locus_tag="IW748_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104075.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
BASE COUNT         4271 a         3334 c         2414 g         5368 t
ORIGIN      
        1 ttgattttcc cttttttagc atccacaatt cttgtttcta attggactgg atattttccg
       61 ccaacaactg attcaaaata taccttacat tctgataaat catatggaag accattttcg
      121 acaattgtag cttccataac ttcagtgttt ttattgcctt gtcgaacttg aatcatcccc
      181 acgtaattat agggttctgt tgtacttaat atgacattcc actttgccat taaatcacct
      241 ccctattttg gtggtatgac aatggaagaa attgcacttg cactataata ctggcgatct
      301 aactttccac aaatcatacc taactctgtg ttttgttcat aagtttgcat acgaccatta
      361 gctaagcctc ggataacgcc agtatgtcca taagttccgt ctgcaaacca tgaacccact
      421 tgtccacctc ttgcccaatt aataattgca ccaactacta attgatcata tctagggttt
      481 tgaatcactt tccaaccaac agcagaccaa tcataggcaa taccaatatc agatgctgca
      541 gaagtatttc ctatcacatg agttaaacca tattttgttc cagcacccat gccacaacca
      601 cccagatatc ctgaatattc tgcagacaag ccataacact gaccattgcc aattcgttgt
      661 ccaatcaaag actctaaatg ttttagtcct gcttctccag tagctcctcc tggttttaaa
      721 tctttaaatt tgttatacca gttaactgca taatcttgac gttcaggatg tgtcgctgcc
      781 ggacgttcat agtttcgttc aaaagcatat gctgcttgtc ttggatcagt acaggcctta
      841 aatccatcaa ccgtcgtagg ttgtactacg cccatccatt gtccatttgt aaatgtccaa
      901 ataagcaatc gaacttgtgc atctaagctc gttattggtt ctttaatacc tgcagcatta
      961 aataagtttt gaacataaac ttttccatcc catgttgctg gaccaacaag aggatatgaa
     1021 gaaccatccc actgaactaa tccgtatgct ggtccaccta tttgaacagt atcaggatca
     1081 aaagttcctc ctgtttcttg ttgaatgttc cccaatattc cacatgcaga ttgtttcgta
     1141 aatccgttat tacacaaaat atcgtatatt ctccaagctc tcttttctgc atctgttttt
     1201 aattcatcag gatacccacc aggtgtttct cctccgccag atggaccgcc attttgtcct
     1261 ggtatcactt ctttacctct aacagtaagt tttccctgaa tatctaaatc accgaaataa
     1321 tatgcttttc cgtttcctaa taacacaaat cctttttctg cagttggaga aattaaaatg
     1381 tattttccat cgccatttgt acgtattacc aaagaatttt cctcaagagg tgtaggtgtt
     1441 acagcatttg gaaatggatt tcctgctgaa tctgttgttc cgatcgttcc aatggattct
     1501 tttctattcc agaattccat accttttttg gttagctcca tgattttttt gttttcattc
     1561 caaatttgaa gcgtgccttt tacaagtttt aacacatcac cataagcatt aaaggaagtt
     1621 tcgaatactt ctgcatttat tgttcctact ttgatgaagt cagcaacaat ttctcctttt
     1681 gatgtgatag caataccgaa cgggccattt actccgttgt ctgaataacc taagccattt
     1741 aaattccaac gccacactcg ctttgcattc gccacatttg gagtatccat gataagaatc
     1801 tctgatggag ctttttctgg acgaaaaacg acatgcccac cagaattacc agtgatccat
     1861 gccgtcgcat tcaacacatt ttgaactaac gtttctgttc gattgtcgat tttctttttc
     1921 aattcttgtg cttgagtatt tactgccgaa gtgtacagtg ataaatcatt acctaaaaca
     1981 atatccttat atttcccaag cgttgggaaa taagtgtatt cgaccatacg ctcttttatc
     2041 tcgatatcta gctctttcgc cctaacatga gcgacatcac caaagtgtaa agacgctagc
     2101 tcttgataca tgtcaccgta ttcgagtgtg tgttccaacg ccaccatact gactgtatga
     2161 gtagcttttg gctcatggat acgatcattg tcaaataagg ttttgcccca tttgatcaat
     2221 tcatcaactg ttttacaatc tccgttttca cgtttggcaa tacgtcgatt ctcgttggtt
     2281 actccatcaa tttctaaata accatattcg ataggttctt tgtcttcatc ataatcgtta
     2341 tcaggaacac cgccaaccaa atacaaacta ttgacaattg attcttcatc aatttcttct
     2401 tcgattgctt ctaaattgat gccaaaatca atccgaaaac cattatctga tccaatctgt
     2461 tttaccagtt tcaaatcata gttatccata tcaagttccg cgctagtcac gccagtcaaa
     2521 ttttgattac cgttatttga accaatgatt gcttcaactg gcgcgacttg tcttgctgta
     2581 aattggtgcg ttgtccctac attcgaaaga tagttaaatt tttgatcaaa tgccaaactg
     2641 tttttaagat tggtcatgat ctgagaccca ttgccgttat ccgtaaacga cttgacaata
     2701 aagttcttat tggccataaa accaatatgc cgagctgtta cagaaacgga ggtcaaattc
     2761 tttttgacat tataaatttc aaaatattgc catgatccat ctggtacctt cgcttttata
     2821 aaatttcctt tttttaaata agagcgatac tgtccgcttc ttgaatagtt accataaaaa
     2881 cgatattggc cattcagcac acgattgatt tctggcaaat cttcccaatc aactaacgac
     2941 gctccgttca cgctcaaatc atctggcatt tttttataag cataaataaa ttcttgcgtc
     3001 acagatacac actcctattc caaaaccgca cttctttaaa ttttccggat atttttacct
     3061 gattccattc aggctgcaat actggccaat caccacgcgt aaataaattc aaaccttctt
     3121 gtattgcttt tccaagctga gtatcaacca cgattgttgc agctaacgta ttaagtattg
     3181 ttaggctttt atcaccgaca gcgatcgtta tatctcctcc gttcgattct atttccagat
     3241 agggatgtgc aatttcgtca ccatgatcaa aaatatccat aacactagaa tgaaaaattt
     3301 taggtgcttc accgattttt cttttgagtg gctgacaacg aaaagtaaca tcgaatgtat
     3361 aaaagaagcc ccactcgttt tcgaaaggga cttctttatc catgctgcag atagcctcta
     3421 gatacttgtc tggatcatta tgtgtgatta attggctttt accagtgagc catcgtttga
     3481 cttctctcag tttcgaatgt ggaatagtga tcccttctat ttccaaatca aaaggttcat
     3541 agtcatcaaa cgtctccgtc aattctccgc ttcggccctg aatcgtgtaa gtttcgtatc
     3601 gcttgttcgg cataatatct ggaagctctg cctcaatgat gcaattcata tcaattactg
     3661 cattccgatt tttccaaata aaatttggtt catctggatt cataaattgt cgactcaaat
     3721 aggaacacct cccaaatcac gaatagcctg tttgtttgct ttagcaaatt tacgattcat
     3781 acgatctaac tcagatggat tgtttgcatc gactttgcca atgtgtatat gctgttcaat
     3841 gctacctcca gaaactcttc cactgatccc tttgcgtttt tcttcgtctg ataatggagt
     3901 aactgtggtt ttaccattct tggcagtcag taactcaggt ccagcttcac caacaatcgc
     3961 ttgtccattg attagatgac cgccttgtgc taaataaggc agttttgaaa tagaaaatga
     4021 ttttccacct acaccaggaa cccattttgg tatctttata ttattcaatc caccaataaa
     4081 tccattgatc aatccaatca tggcattaat aggagctttc cctacagcaa caataccatc
     4141 gaaaatacct ccaaaaatat caactagacc ttgccatgct tttgaccaat tccctgtaaa
     4201 tactccagtg ataaaatcta gaaaaccact gaaaattcgt tttccagcat tataaaaatt
     4261 attgaaattt gctataacac catcgaaaaa tccaccaaaa taaccactta aaaaattaaa
     4321 tatttcaaca gctacatctg aaacaccttt gaagaaagaa ttcactccat catgaaacca
     4381 tttaacgtta ttatatgccc aaattagtcc agtaactaat gcgccaatag ctataactgc
     4441 taatgcaaac cagcctcccg acattccaaa taaaccagca agtccttgcc aaactcctat
     4501 aaaactttta acttccccaa cgatttttgt aacagatccc ataagtgaac caagaacgat
     4561 tagtactggt ccaactgctg ccgctatacc agcaattgtt atgatccaat tctttgtatc
     4621 ttgatctaga gacccccacc atttagagaa ttcctgtaat aaggaagttg ctttttcaaa
     4681 aaaaggcaaa aggcttactt ggactgcttc acctacgtca gccattgcta attttgcatt
     4741 gttcatagct tgatcggctt gatcaattgg gtcgagagta gcatcgaatg tgtctcctac
     4801 agctccccca ctttctccag cagtttttgc taaatcttcc aggttcaaag tacctctacg
     4861 aattgcatcg gccattctag gaccgccttt agtcccgaat acttctgctg ctgcattgat
     4921 ggcttctgtt tcagaacctg cattttttac cttgtcttgt agttcttcta gtccttgact
     4981 taaagatttt ccgtctttcg cataagccac tgttgcttta gataaactac ttaaagctgc
     5041 gcttgaatca acaccagatt gctcaaattg tccaagtaaa gtgacccctt caccaaagct
     5101 caaccctaat tgtttaattt gtggtgcgcc atcaattgct ttttgcatca agtcgtctac
     5161 agattgacca gtattctgcg atgttttcgt tgttacatca aggacagaat tcaaatcatc
     5221 atattctagt ccataagcat caatagcttg tcgagcagat attgctgatt gcgaaacatc
     5281 tgtatcattg atttcagcgt actgtaggag ataattggtt gaatcttcca gttttttatc
     5341 catgaatcca aattgtgtat ttacctcacc aatagcttct ccaaccgttt gtaaaggtaa
     5401 atgagtattt gaaccaacgt ttttgaaaga ctgtgaaagt ctatcagctt gatcacctgt
     5461 tgctccggtt tttgtaatga tggtatcaag tgcctcgtca acttcaccaa atgctgcaag
     5521 tcctgctgct cctgccgcta caataggcgc ggttactcca acagacattt tttcaccaac
     5581 gccttttact ttctcacctg tttcttcgat tttctggagt ttttttgctg tatcaacaga
     5641 aacgtcacct tgttctttaa gtgcatcgtt cgtctgttct aatgctgtac gaagtttatt
     5701 ttctccggtt tcagaattga gtaattgttt ataaagtttt tctgattcag cagaatattc
     5761 accggtttct ttgacagatt tttcatattc ctctctcagc atttgagatc gttgttcagc
     5821 caatcctaat tgtttttcta atttttgttt agccgctcgc aatttttctg tttgagttgc
     5881 atccttgtcc atcgcagaca cttggttttt gtactcggca gcggctaagt tcatttcttt
     5941 gttgatttct ttgattgttt tcgaataact gacttcgcca ttcgttttaa aattaagaac
     6001 gacatcagat tctttaccag ccatttatct tagcgctcct ttctaccacc aaggcgattt
     6061 atccatagtt acagattgag gtggttcaaa atcggtgttt tgttgtaacc actgtaaata
     6121 agatttgagc cacaagttag gtgtagactt taaaaagaaa ctctcactcc aattcaatag
     6181 agtgagagca acgtaaatat aaaaactcca aggagttcct atctcttccg attctttttg
     6241 tttttctttc tttttgcttg cgctttttga aagtcttgtg gcttcttgga tttttttagg
     6301 tcttccacct gaaatgtctg gctagcaaaa atttccatac aggtactata agcagacaac
     6361 acctctccac tcattcccaa aaatttgaaa atagtttctg gatcttcctc taaaccacca
     6421 gtacgcagca tggcataaat caaggcacgc atgattttta gatcactagg cgataagtta
     6481 gcagaagaaa tttgtccttc ttttttagat agcatggcgt tcatatcaga ttcaaattgc
     6541 gaataatctc caccatacac atctgctata aattccatcg tttgcatagt aaatgaaata
     6601 gggaattctg cgccttgaat aattacaaca gcagaatttt ttaagtcttc aacgtgaatt
     6661 ccataatcag ataaccgagc cattaacccg cacctccaac tttagccaat gttttccact
     6721 gttcttcatc gtatacaggt tgagcaatga atttattaaa taattctaga gaagctccgt
     6781 ctctatttga atcaaaactt gcatacatca cattattgta tttcagtccg atggaaacca
     6841 gatttgcagt tacatcgtca atttttgttt cttcctctgc agtggcatac tcttcgtcaa
     6901 tcacattgga tagttgtgta ttaggatacc aaactgcttt tttaccgcca ttttcaatat
     6961 ttccaataaa tccaaaggcg aagtacggaa attctcgtgc ggtatttttg ctaaacgtta
     7021 ctccgctttt agcaactaat cctttgattt catccatcac ttctatcgga atacccacgt
     7081 gatccaacgc aagttcatgt tttgtttctc gcgacacacg acgaaacatt ttgctggatg
     7141 cccatttttc taatgctgtt ccattccctt tgacaccgat ttttgtggca attggcaatc
     7201 gtaaaacttc actataagtc ggtgcagttc caacagaatc tggcgttgcc atcatcgcaa
     7261 ttaaaatgtc gtccaatcct tcaaaataaa aagtatcttg ttttcccaat taaaatcacc
     7321 cttcccataa atcaagtatt tttctagtca taatttcttc aattttttct ttgttttgtt
     7381 catacgtacc acttgcaaaa tgttgggcac gttgatttgt tgtgccattt tcggcaaagc
     7441 gccagtaaaa ggccgtctct tcaaattgaa ctttcacttg atcgttttca actactactt
     7501 ttatttgatc gcgcatatgt ttctttttta agagcgactt aggaatatta ggaagcaact
     7561 gctttagata aaagttagct gcttcttcta aagattccaa cgataatttt ttcggatcaa
     7621 cttttgaaag gtttcctaaa taatccgata tttcagaaaa accattctta ttacttggca
     7681 ttttccacac acctcacata tgtgtaatag ttggttacgg tatcgtcgtt ctcatcacct
     7741 tgtatacctg taaaatctga atatggaatg ccagcatttt gcagcgcttg ttcaataaca
     7801 actaaatcct gttctgttcc aagtgtaaaa aaagagactt ggtaataagg taatttttta
     7861 tagactttac cggatgccat ttttttgctg ttactcacat ttgagtacac aatgtatgga
     7921 tacatcgtcc ctaatctggc tttgtctctg aacactgcta actttgttga tttcagcgct
     7981 gttttcaatt catcaaagct aatcgacata agctaaactc aactccattt ctcttgcatc
     8041 gggattcgta taaatgcgag taatgttata cgttacagaa tcaattttga gcgcacttaa
     8101 tttctctgtg atggatttat cccatctgac tttaattcgt ctgacaacgt ctgtcttggc
     8161 ttgctgtgat aaatattttt cttgagaagt cacaccgagt tcttcataga atattagacg
     8221 cttaaattcg taaattgtag ttggacgatc gtttccgtct gttcctgttt tgatgtctag
     8281 caattcggct ttccatctga gattattggt ctgtctcttc ggcattttga atcgctcctt
     8341 gcacgataaa tggcgtcatg gcattcatag ctttgtcgag ttcatcctct gaaactctat
     8401 attcataggc aatgccggca accatcaaaa taagatattc ttgttggcca ccagttgctg
     8461 ttttgacata atcttttgcc atatttaaat aaaaagagag caaagaatca tccatgccct
     8521 cttcaaaatg aatatgtgat ttgaattttt cctctaaaga caattcttta gtttgctctt
     8581 ccatcttaac caccaactgg ttttgtaatt tcgtagcgat atactgccgg ttcaaatgga
     8641 gaataaacca attgaccatc tagcaagttg taaatttgga atccaatttg atttttacca
     8701 gagaattttt caacaagttt ttgaatttcc aaggcaccaa taacttcttg aattttaaat
     8761 gcagaaaaat cgccaaaata taaaactggc gtgtctggtt cacccttttt atctgctgca
     8821 tctgtccaat ccacaggata gccaactaat tggtaaccaa ttccaccttc tgcttgtgtg
     8881 aatggccgca acaaaggaaa tccatcatct gttttcattt tttcaatggc agtcaaagca
     8941 gctcgattaa taataaagcg tccctttttc atcacttctg tcactggtgt atttttaaat
     9001 tcgattaatg catcatataa tttttgccca gcacctgaag cagttagatc taaaggtttt
     9061 ttaaatgcta cagccttttt ggctaatgca ccaggatttt catttccagc gtcatcacca
     9121 ttgaacatat aattgatttc tttacgcaca taagcttttt tcaattcttc cacaacaata
     9181 tcttcaactg gaacaccaga catttttaat aattttttag ttactgttgc caaagcatcg
     9241 aattcggcag gattaagcaa aatttcatca aactgaatag ctgtttcagc aatatcagtt
     9301 gaacgctctt tcttgtttac attcgcatct gctttcttca caagaattgg atatttgaca
     9361 tctcctgatg ttcgcaccac tgttccgtat ttacgaagta aattttcttc ttgagcataa
     9421 gtaataactt cagatgcaat tacttctggg acagtaactg aaccgttgcc agcttcaatc
     9481 cctaaagctc gagcttctgc ttcagaaata tttccagcta caaaattagc aaatgctttt
     9541 cgtagttgtt gatctttttg tttcttggtc atttttgcac gtgcctccaa tccgttctta
     9601 attgatccaa gtaatccatc tcgttgctgt tgagtaatca ttccagaacg attttctgaa
     9661 ctatcttcac tgtctgaatc ttcttcttga tcgttgtctt caccagaacg gctttcgtct
     9721 gaatccgtac tattcgattg atcatctgta ttgtcgtctg tttcatcagt tccagaatct
     9781 gcgcctaatt cgtctttaat tccgttcaat tcatcaataa cactgtcaat ttcttctttc
     9841 acggcttcta aatctgcttc gcgtaattct cctgattcaa ctttttcacg taattcagtc
     9901 aatcgttgct cacgacgtgc cttcattttt ttcaataatt ctttatccat gtatttttcc
     9961 tcctacgctt ctagcgcttg attaattttt ttgattaatt tttttctttc ttcgacgttt
    10021 ttttctaatt catcacgact tcttaatgca gcttctgtat cttcgtatgc tggcaaaggc
    10081 acaatagaaa cctcgtataa ttccacttca tggattgttc gaagcattgg ttcagaattg
    10141 tagtcccacg tttcttcagt cggcacaaaa ccaaaactac attggttgat gtctcctcgt
    10201 tccatagatt taactaagtc ccttgctacc gttgtgtccg gtaaatcaac ttcgaatttc
    10261 aaaccacgtt catcttcttc aaggcgaagg gttccgcttt tagtacgtcc tagcacattg
    10321 gaccaatcgt gattgaacaa acaacgaaca tccgaattac taattgcctt tgagaatgca
    10381 ccaggaacaa tcacttcgct taagtcatcc cataaaagtg tttgactgtt gaatacagca
    10441 gcgtagccgc taatggtcct cgtatgagtt tcttcatcgg aacgtgttga aagattggtg
    10501 atgtcaatcg tgcgaatttc cttcttcttc atttccatca cctcctttca agtcttgatc
    10561 attcgttggt aaggaatcat ctgttgcatt tttctggcca atcctagata agtcatttga
    10621 aatatagata gcttgtgttt ctggagtatt ctgtttagga aaaccaagca tttctgccac
    10681 gttatctgga cttgtaatcc cggtacgaac gatgttgtag ccaatatttg tttttgttga
    10741 gtaaggaaca aaatccaaaa tattaatttt ccattccact ctatagccag aattaggcat
    10801 aaaaaaaaga gcggtgtaat gttcgctctt gtttttcaat attggtttga ttgctttgtt
    10861 gtgcagatac atcatcgctt tttcaatatc tgtcttcatc agcgattgat acgtatcaac
    10921 attgattcct agaaattttc ctaagtcttt tttgtaaaca cctaaataat tcaaaattgc
    10981 cgcgtcatca acaggacttt ttaatgtctc gatggaatat ccttttccca aaggaatcat
    11041 cttaacagaa tgatcactct cattttgcgt tccttccagt tgatccaata tagctttgac
    11101 aatttttgtt tgggcgctat tatttggatt gatgtgggcg tctagtttaa gcatgaacgc
    11161 aagtaaaccg cctttagtat atttgtccgt caaaactttt tcagcgctca gaacgccttc
    11221 tagagtgttt cttgcaagat caattattcc agcacctttt aatgaatcag ttccgatgtt
    11281 cttaatgtgt cgaatcattt gaccaggtat tttttgacca ttcatttcaa attcttcttg
    11341 aagtcgttca ttgattttag ttgttacacc gtacgccaaa tgaagctgat ccctatccgt
    11401 taatgggaat gtctcaccat tgatcaataa agtatttgtt tccaatttgg taaattcgaa
    11461 tcctgtcaaa taatcattgg gactcttcaa aatatttagt aagtgatgat ctttgacttc
    11521 gttgccgtcg ggaccaataa caattggtgt ggccaatgcc acctgattag atatgtcttg
    11581 aactaattca taaacatcag aagattccat gatagatgaa tccgtcacat atctttgacc
    11641 ataacgtgtg tagtgtccaa aaatatcctc gatatatcca cgtttttcca taaaagaata
    11701 gactgcattc gataaccgat cacgtaattt caattttttt caccgccttt ctatttatct
    11761 atagatggaa cttaggtaat catctaactc atctgaatca atatctgtca tttgattcat
    11821 cgtttcctta tgaccacaca aaaacgccac gaacccatca atctttttct ttgattgacg
    11881 tttacttggc gctttttgtc cattgatgtt agtaattgct acaacgttca aagtgcaata
    11941 aaggaataat ggattatcaa attgaattcg tttctcataa aacaaccgtt cgacatcgtc
    12001 aaaaggagca ttcaatactt taggatattg agcaacttca acgcattcca atcctaagtt
    12061 ttctaatttc tcaacaagtt tgtcactcat cgctggatca taattcactt gctgaatatc
    12121 atataaattc atgcagtcct ctatgtactg caaaatctga tcttgatcaa tcatttttcc
    12181 gtcgcaaaat tcaacgaaac cttgttcagc caaatcactg taaggaacat tatcttcttt
    12241 ttctcgaaac tctaaatctt cattcggaat aaaataaagt tgctttactt taagaaccgc
    12301 tttaccatct tcatcccatg ttgggaagtt taaagataca caggtcaaat ctcgtgtacg
    12361 tgataaatct aaaccgatat aacatggttc gccacttaaa tcaccaagtt cttgtgtggt
    12421 aactaaacat ggctctactt gatcctgttc aaagaaatta tccgcgccgt tcacaaatac
    12481 atccaagtgt ttcgttagaa attcagcttt ggaatgagct gaccgttgcg ctgttttaaa
    12541 tgccgattct aaagcagata aatcgactga tattccccag ttaggattgc acatttccca
    12601 gacttttcta tccgtccaat cataattttt atttggctca tagattagaa caaagtttga
    12661 atcattatca tcacgtttca acacttcttt tgcttcgcga tatacacgca ttccaacaga
    12721 cgacgaacct ttcccagcag ttgagatatt aaacatcaat ggctgtggta aagatatctg
    12781 agcagactta aaattgtcat actgttccat tttctcctgt ttatgcaatt catcgttcaa
    12841 tacaaaatat ggattggatc cctcgatatt atcaatattc ttggtttgaa caataaattt
    12901 atttgagtaa gccatatctt catgaatata gtcataagta atactagaaa cagttccctt
    12961 tggcccccta aatattttag ttccttcaag taggattgga ttgtttaaga ttgtaactgc
    13021 aaaaggtttg gcagcatatt gcgcttgagc aaaatcagat gcgcaagcat agcaatcaac
    13081 tgataacgcc ccttctccgt acattgcata tcctaatgaa ccgacagcaa tcaatgtttt
    13141 accattcttt tttggaatct ggacataagc ctcacgagta acgcgtacaa tttggccttt
    13201 ttcattttct ttaacccaac cataaatcca agaataaata aatttttccc agggttctaa
    13261 aagaaacggt tttcccacca tttctccttt tgtgtgaaca ataaatgatt ctacccaatc
    13321 catcatttca ttcgcgcgat ctacatcaaa ccaaatatct tttctttttt tccactgata
    13381 ccagcgatct attgctaaac gaacggtctt aggatacttc ttaggtttct ttcttacttc
    13441 ttttgcaaat aaatcagcat aatttacacc aggttcaatc atttttcagc acctgccttc
    13501 ttacgccatt tatttctgtg cgctgctagt tcatctactg gctttacttc tgggcgtgta
    13561 atttcttcat cttttcttgc ggtcgatcct ccagcaatta gtctaccagt ttttgctttg
    13621 tttgttaatc ctaataaatc tagagctttc atcttcttat ctgcccaagt ttctacttgt
    13681 tgtgccaatg gatgttttga ttgattagta gctcctgatt tattcgtgaa tttttgcgtc
    13741 tccggaaatc ctttttcctt ccacaataaa tatttgtgtt ggtaaatttc aaaaatatcc
    13801 aagtatgatt cgattaatgg atcaagagtg atggtgtaca aatcagacaa attcattatt
    13861 tctaagatac gagctttttc agcacttact ttttcatcaa taatcgcttt acgttgcgct
    13921 ttagtcgtca tttttgtata cacccccctt tgttttttga aaaatttgac ctaacgatgc
    13981 gcgtgactcc ccgctaccct atctccccac gcgaaaaaat tttgaaaatg aatagggggg
    14041 cttcaattga aatacgaagg aaaaactttt ttgtcttccg tttcgttttc aacaattgga
    14101 tgacattttg aacataatag catgagattg tttggatcaa gcttaagcag ttcgttgtct
    14161 ttgattggta caatgtgatg gacgtgtgct ctctttccaa atatgaactg accacaccgc
    14221 tgacaatgac caccttctct ttcataaata aattgacgca tatctttcca tgcttgcgtt
    14281 cgatagaatg gtttgttctc atgatgataa acagactttg cttgctgctt cttcttgcgt
    14341 gatctgcttg atctcttgtg ttcagtacag tagataccct ttgctatttt gttcgtgcat
    14401 ccgtcaaact gacagtattt cattctactt cacgaataag atttatgata tcgccttttg
    14461 cacggatagc accaggaata tcaataccat gtttcttagc atatgcacgc aattcttttg
    14521 cagtcatatt gtccagttca tccgtatctt cagtggactg atcattagta gcttcgtctc
    14581 catcaaaatt agcagcagtg tttccatcgc tatcaagaat ttcactgcta tgagctatga
    14641 gttcgccatt gacagcaaca aatgatttgc catcactcaa aattcctttt tcttctttta
    14701 cttcaaaatt tggttcttga tctttcggaa caactactgt ccttttcttt tctgaatccc
    14761 aatactctgt tccactaatg gatgttctaa ttttgatcat tgccatttta attctctcct
    14821 ttgtaatttg tacggattac ttttgcgccc attcgctcat accattcaat cgtttcctgc
    14881 agattgggta gagtgtgtga caataaagaa ataactaacg tattcattcc agttctgtaa
    14941 tcatcattct ccacttgcac atgtgcatga ttaccattcc aattttctct gtacggacta
    15001 ttctctgctt caattgcttt cttataaact ttagccaaac ttgattctac ttcaatacta
    15061 agtaccgctt catgaaaatc attcatttgt ttactctcct atcagatatt ttgtggtatg
    15121 atttataaaa aagatgcgag gtgttttaat gcttcaagaa gttaatatta agtatcgtga
    15181 tcgtcaaaat acagagacaa tttatttgcc aaatagttgt ccgcattgcg gaaatataat
    15241 gactccgtat gtgtattttg gggtttcatc atatagtgcg ttcgataatg atagaatctt
    15301 tggcgtatta gtacagtgta taaatgatga gtgcaacaaa ttttatagtt tacaatttat
    15361 aaatacgttt aatgaagatg aaggata
//
LOCUS       DACENJ010000051        14992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.051, whole genome shotgun sequence.
ACCESSION   DACENJ010000051 DACENJ010000000
VERSION     DACENJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.051"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IW748_001157"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IW748_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1104076.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IW748_001158"
     CDS             complement(322..891)
                     /locus_tag="IW748_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1104077.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IW748_001159"
     CDS             1077..1979
                     /locus_tag="IW748_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1104078.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IW748_001160"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IW748_001160"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1104079.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IW748_001161"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IW748_001161"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1104080.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IW748_001162"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IW748_001162"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1104081.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IW748_001163"
     CDS             8547..8732
                     /locus_tag="IW748_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1104082.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IW748_001164"
     CDS             complement(8794..9990)
                     /locus_tag="IW748_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1104083.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPLMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IW748_001165"
     CDS             complement(10160..11596)
                     /locus_tag="IW748_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1104084.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IW748_001166"
     CDS             11889..12698
                     /locus_tag="IW748_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1104085.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..14986)
                     /locus_tag="IW748_001167"
     CDS             complement(12884..14986)
                     /locus_tag="IW748_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1104086.1"
                     /translation="MRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLGYLQ
                     IVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTAKDL
                     LEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQYSAM
                     VNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAERASELPG
                     VSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYLEKQ
                     YEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKRNYQ
                     TLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDPGSV
                     VKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELSSNT
                     YMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESPGIS
                     RSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQPHLV
                     KAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAKPLA
                     SAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQDHIN
                     LTIAKEIMDAYYDMFMAK"
BASE COUNT         4376 a         3178 c         2660 g         4778 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattaaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tc
//
LOCUS       DACENJ010000052        14926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.052, whole genome shotgun sequence.
ACCESSION   DACENJ010000052 DACENJ010000000
VERSION     DACENJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.052"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1097)
                     /gene="tgt"
                     /locus_tag="IW748_001168"
     CDS             complement(<1..1097)
                     /gene="tgt"
                     /locus_tag="IW748_001168"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1104087.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREA"
     gene            1315..2013
                     /locus_tag="IW748_001169"
     CDS             1315..2013
                     /locus_tag="IW748_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1104088.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2181..4820)
                     /locus_tag="IW748_001170"
     CDS             complement(2181..4820)
                     /locus_tag="IW748_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1104089.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(5439..6470)
                     /gene="queA"
                     /locus_tag="IW748_001171"
     CDS             complement(5439..6470)
                     /gene="queA"
                     /locus_tag="IW748_001171"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1104090.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            6663..7838
                     /locus_tag="IW748_001172"
     CDS             6663..7838
                     /locus_tag="IW748_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1104091.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            7842..8483
                     /locus_tag="IW748_001173"
     CDS             7842..8483
                     /locus_tag="IW748_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1104092.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            8480..9397
                     /locus_tag="IW748_001174"
     CDS             8480..9397
                     /locus_tag="IW748_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1104093.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            9394..10065
                     /locus_tag="IW748_001175"
     CDS             9394..10065
                     /locus_tag="IW748_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1104094.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(10606..11094)
                     /locus_tag="IW748_001176"
     CDS             complement(10606..11094)
                     /locus_tag="IW748_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ1104095.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11295..11561)
                     /locus_tag="IW748_001177"
     CDS             complement(11295..11561)
                     /locus_tag="IW748_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1104096.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(11561..11824)
                     /locus_tag="IW748_001178"
     CDS             complement(11561..11824)
                     /locus_tag="IW748_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1104097.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(11817..12089)
                     /locus_tag="IW748_001179"
     CDS             complement(11817..12089)
                     /locus_tag="IW748_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104098.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12300..12485
                     /locus_tag="IW748_001180"
     CDS             12300..12485
                     /locus_tag="IW748_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1104099.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(12627..14450)
                     /locus_tag="IW748_001181"
     CDS             complement(12627..14450)
                     /locus_tag="IW748_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1104100.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4372 a         3179 c         2825 g         4550 t
ORIGIN      
        1 gcttctctaa attcaagcaa attatcatcc atgattgctt gacgtacttg tttcattaga
       61 ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa tcccaaatgt ttcatcacat
      121 ttgatcaaat ggcggatata ggcacgtgta tagtttttac atgtataaca atcacatttc
      181 tcatcaattg gacggaaatc atgggcatat ttcgcatttt tcactactag acggccttga
      241 gaagtcatac atgtgccatt tcgagcgatt cgagtaggca agacacagtc aaacatgtct
      301 actccacgga taacaccatc gatcaatgaa tcagcagcac cgacacccat caagtaacgt
      361 ggtttgtttt cggggatcaa cggtgtagtg aattctaata cacgattcat ttctgattta
      421 ggttccccga cagataaccc gccgattgag taaccaggga aatccatccc gactagatct
      481 ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt ggatgattcc aaataatcct
      541 tgacggtctg gattagcatg agctttcaat cctcgttctg cccatcgaga cgtacgttct
      601 actgattttt tcacgtagtc gtaactttca tcgaatggtg gacattcgtc aaaactcatc
      661 atgatatcag agcccaattt attttggatg ttgattgctt tttctggtga taaaaacata
      721 cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt cttcgatatt ccgcatatca
      781 cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa tcggttgatc ccagttcata
      841 aatttatgca gtccgccagc ttcttctact aagtcttcac cagggcgtag ccataaatga
      901 tatgtattac ttaaaataac gccagcaccc atttctttca actcttctgg ggacattgtt
      961 tttactgtag ctaatgttcc tactggcatg aacattggtg tggggaaagt tccgtgtggc
     1021 gtgatcaatt cccctaaacg cgcgccagta tgtttttctt tcttgattaa acgatagcga
     1081 atggcaggtt gcgtcattca tttccctact ttctatataa aatatcttga caactcccac
     1141 catcataatc gtttcaagtg ctttttgcaa gttccctcgg cttccttcta ctttatttaa
     1201 tattcttttt tttatccttt ttttatgaat aacaaaaaaa agaaaaacat tatgacagaa
     1261 atattttttg atacatggta tattggtgtt attaaagaaa aggagggaaa aatcatgcaa
     1321 tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa aaggacgttg gggacaagct
     1381 gttttactaa atctaatccc tacattgatt ggtattgcta ctattcttct tattatgatt
     1441 cctattatct tgtttgccac caccatatat gatggatcag cgagcgtctc agaagcgaca
     1501 acttcaggag gcggtagttc aggaggcggt ggcggaatta tttccagcat cattagcgct
     1561 ttgttcatga gcggtatttc ttggacttat cttgatattt tacgtggaca aaaagatcag
     1621 atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt tgtttatcgg cggagtgatc
     1681 agcttagctg tgctgatctc gatctttaca acattatggg gtttgctatt agttattcct
     1741 ggaatcatta aaagctattc ttattctcaa agctatttta tttactacga tatcgtccaa
     1801 gaaacaggac aaacacctaa agtactggat acgatcactg caagtcgaaa attgatggat
     1861 ggttataaag gacaactttt ctggttagat ctttcattta tcggctggca tatcttagca
     1921 ttagcaactg ctggtattgg ttatctatgg ctcagcccat acatttctgc aacaaaagca
     1981 gcattttatg aacaattgcc aaaagatatt taaaagagag tgtgacttgt gaccgagggg
     2041 gtggcaggag aacaccgttt atcgagaggc agtaaaagaa acattcagcc cattagcaaa
     2101 aaggaggtgt gacaacattc atgtcacacc tcctttttgt atagttccag taaaaagaaa
     2161 atcaacttag aaacagttaa tcattcctct tcttctgctt ccggtaatcg atggaacttg
     2221 atcagttcat tcaccgcgat tggtacgata gataaaatcc ctaccaagaa ccagtgattc
     2281 aaactgatgt gtactaggcc gaatagttct tgcgtaaatg gcagcaaagc aacagccgtt
     2341 gtaatagcta atgccagtaa cgccatttct accaaagctt tattggtcgc taaattgact
     2401 ttgaagatcg attggctgct gcgtacattg aacacatgca gaatcgatga ataagctaag
     2461 atcaagaatg caacagtttg accgacatgc tgacttgcgt ctacccatgg gctgatagta
     2521 tcgacataac tacctaaata ataaccaaac aaggttacga tggtgaagac ccctgcattt
     2581 atggcaattt tttgcaataa acctcgagca aagatccctt catttttcgg aatgggtggc
     2641 tgatccatga tgccgtgttc cgctggctct cttcctaaag caaatcctgg aagtccatca
     2701 gcaactacat tgacaaacag tagttgcaca gctgtaaatg gtgctcccca tcctaacagc
     2761 atcgcaatca aaacaataaa gatctccgaa atattacagc ttagcaaaaa gttgatagct
     2821 ttacgaatat tctgataaac cgaacgtcct tgactgacag cgtctacgat cgtcgcaaag
     2881 ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca catctgttcc tgtgatcccc
     2941 atggcacacc caacgttact tgctttcaac gctggtgcat cattcacacc atccccagtc
     3001 atcgctacga ctgcacccgt acgttgccaa gatttgacga tccggatctt gtcttctggc
     3061 gttacccgag catagacaga gagagctttg acacgcgcat ctaattcttc gtctgataat
     3121 tgatgcagtt ctgatcctga aagtgcttct gacggatcat tcaagatacc taattctttc
     3181 gctatggcac tggcagtcac cacatgatct ccagtgatca tgactgtttt gatgccagcc
     3241 tttttcgctc gcttgattgc tcctttactt tctggacgtg gtggatcgat cattccgatc
     3301 agaccaatca aacgtaagtc tttttccagt gcttcagaag aaatatcttg cggttcttcg
     3361 tcataagtag cataaccaac agcaatcact ctcagtgccc gtttaccaaa gcgatcatta
     3421 acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg gagcaagaac atcgaatgct
     3481 cctttggtga ttgagatgta tttttcgccc atttggtgaa ctgttgtcat catttttcgt
     3541 tcagaatcaa aaggaagctc gtttactcgt ggatattttt cttctaattc tgcctttgta
     3601 tgataattct cttcaacagc ccttacgatg gctacttctg ttggattacc tttgataaca
     3661 aggtcgtcat cttcttgctc gaccgtcaca tctgtacata gtgcagccat tttcaatatt
     3721 tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta cttcatcttc tttgctccat
     3781 acccggcgga cgcgcatttc attttgtgtc agcgttcctg ttttgtctga gcagataaca
     3841 ttagcggttc ctagcgtttc aactgctggc aactgtcgga tgatcgcatg tttcctcgcc
     3901 atcttctgca caccgtatgc aagtgtcaac gtaacaataa cagtcaaggt ttccggtaca
     3961 gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa ggagtggttc tccttgcagt
     4021 tcaccaatca caaatactaa agccgcagcc gctaaagcga tcatactgat tcgtttgcct
     4081 agctgattca atcggacctg taaaggtgtt ttttgcatag tattttcgtt caaaagatcc
     4141 gcgatcttac ccatttctgt agccatgcca atggcagtga cgatcgcttt cccacgacct
     4201 gcagcaaccg tacatccttt aaataccata ttaagttggt cacctaatga ctcatcttct
     4261 tcagcaataa atgctgcatc tttttcaact gcttcacttt ctccagtcaa cgccgattct
     4321 tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg cagaaatagc atcaccggac
     4381 tctaaaacaa gaatatcacc cactaccagt tgctctgact caacagtggt cactttgccg
     4441 tctcgtatga cagttgccgt ttgtttattc atatcttgca aggattccag tgctttttct
     4501 gcgtttcctt cttggacaat cgccaatacc gagttgatca caacgatagc gatgatcagc
     4561 agtccttcaa ataaatcccc atgttctgta gcaaatgcgg tataaaagga aatcaccgct
     4621 gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc gaaagaattt tttgatcatc
     4681 gattctttcg gcgcctcatc gaatttattc aatccctgct tttttcgccg ttcatccact
     4741 tcttgtgaag acaacccagt atctggatct gcttctgtta ccttctttac ttcttctatt
     4801 gtctgcttgt attcttccat cctattcctc ctttaaaatc ttcgtacaaa aaaaggtact
     4861 tattttacta taacatcctt tttttattta cttcaaataa agggcttatc gattatttgt
     4921 taagaaagcg taaaatttaa agtgcttata tttgcaaacg ctaccaaagg atgctattat
     4981 atagatgtaa ggaaggagga taccatcaaa tagacagaag aatttctcga tagaacagtg
     5041 taagcaaaca aagttgttag aagtacaggt ttcacccaga catgcgtgta gagtacaaac
     5101 ctataaatag tgaatgcgta tggatgtttg gatcacgaag tatccgaaac aagaagtaat
     5161 gcgatacgta acaattgaat aacgtaaacg tctgaaaatt gaataggaac aagagatgtt
     5221 tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta acatgaagaa attcaacacc
     5281 gtgctttgca tgtatgatgg aaagttacaa tcatttctgt ttatttgaat ccttccgagg
     5341 tacaagaaag ccgagcttat cgagagctcg gctttttctt tgtcataaaa tctttgtaaa
     5401 gcttttttgt tattcaaaac tagcggcttt tcataacctt atttcacgaa catcgcatct
     5461 ccaaaactaa agaaacgata gcgctcttca atcgcatgct ggtaagcaga taaagtcaat
     5521 tctcttcctg caaaggcact aactaacatg actaaagtcg attttggcaa atggaaatta
     5581 gttgagaaag cttctaccac tttgaattca tagccaggcg taatgaaaat atcagtccat
     5641 ccgctgtctg ctttgatttc tccattaaat ttcgttccaa tcgtttctaa tgttcgaatt
     5701 gaagttgttc cgacagccac gattttcccg ccatttttac gtacttggtt caatcgctca
     5761 gccgcttctt ccgtcaaacg gtagaattca ctatgcatat ggtgttcttc aattttatct
     5821 acgctgacag gtcggaatgt acctaaccct acatgtagtg tcagatagac taattcaaca
     5881 ccctttgctt cgatctcatc aagtaattct ttcgtaaaat gcaatccagc tgttggcgct
     5941 gcagcagaac cattttcttt cgcatagaca gtttgatagc gatctgggtc ttccagacgt
     6001 tctttgatat atggaggcaa tggcatttct cccaatgatt caagaatctc tagaaaaatt
     6061 ccttcatact caaagtcaat gatccgtccg ccgtgttcca attcttcttt cactactgct
     6121 tgcaatctgc catcaccaaa agagattctt gtcccaactt tggcacgttt cgcaggtttg
     6181 atcaatgtct cccacgtatc tccttgtgta ttcgttagaa gaaggacttc taaatgcgca
     6241 ccagtatctg gtttttctcc atataaacga gcaggcagaa ctcgtgtatt attcataact
     6301 agcgcatcac caggctgtaa ctcttcaatg atttcatgga aatgtttatc ttccatctct
     6361 ccagttttcc gatccaaaac aagtaaacga gaatgatcgc ggtcttttaa tggtgtttgt
     6421 gcgattaatt cttccggcaa gtcaaaatca aaatcttctg ttgttaacat atcgtcactc
     6481 actttctatc atgtgcttca tcattctatc atgtttttcg ttctgttggc aaaatcagca
     6541 ctttttcact tctatttttt gttttcctaa ataaaaaaac taccctattt gtattttttc
     6601 attgttttag gcatactagt aatagatagt tcttcattga aaatagaaaa agaggtggca
     6661 ccatgattga atttcaaaat gtgtcaaaaa tttataaagg tggaaaagtc gctgtagagg
     6721 acgtcaatct ttcatttgaa aagggtgagt tcatttgttt tatcggtacg agcggaagcg
     6781 gaaaaacgac gacgatgcgg atgatcaacc gaatgactga tccaacacaa gggaaaattt
     6841 tgattgatgg aaaatccatc caagaaattg atccagtgga attgcgacgt cagatcggct
     6901 atgttatcca aagtattgga ttgatgcctc acatgacgat ccgtgaaaat atcactcttg
     6961 tccaaaaatt gttgaaagtc agtcaagaag aacggaataa aacagctgaa aaaatgattg
     7021 atcttgtgga attgccccgt gagatgttag atcgctaccc acatgaatta tctggtggtc
     7081 agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa tcaagatatc atcttgatgg
     7141 acgagccatt cggtgcgctt gatccaatca ctcgtgattc cttgcaagac cttgtcaaag
     7201 atttacaaga gcgtttggga aaaacgatcg tatttgtaac ccatgacatg gatgaagcga
     7261 ttaaactagc aagccgtatc gccatcatga gtgaaggccg tgtgatccaa tttgatacac
     7321 cagaaaatat tttacgtcat cctgcaaacg atttcgtaga agagttgatt ggagaagatc
     7381 gcctactaca agccaagcca aatgcgacaa gagtcggcga agttatgcta aatacagcaa
     7441 tcacgattac accggaaaaa tcattacaag aagcaatcaa actgatgagg gaaaaacgtg
     7501 tagatacgtt gttagttacc gataattcga atgtattaaa aggatttatc gatgtagaaa
     7561 cgttgaataa aaaacgagga aaagtatcca gtgtcggcga cattttgaat aaacatgttt
     7621 tttacgtaaa agaatccgcc tatcttcgtg ataccttgca acgcatttta aaacgcgggc
     7681 tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt aggtatcctg actcgtgcat
     7741 cattagtgga tatcgtctac gatgtgattt ggggagaaga tcccgcaact actgatgttg
     7801 cagagtcagc tactcctgaa gaatctaagg aggtctgacc tatgcagcag ttttttcatc
     7861 agcacggttc agaaattctc tcaaaaggtt ttgaacatct ttatatttca ggacttgctt
     7921 tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt actgacacgt tttccaaaag
     7981 ctgccacgat tgtgattggc ttaaccagtg ccttgcagac aatcccttct cttgctttat
     8041 tggcattgat gatcccgcta tttggcgtag gtaaattacc tgcgattatc gcactcttta
     8101 tttattcatt attgccgatt ttacgtaata cgtacattgg gatgaaaaat gtcgattgga
     8161 attatcggga tgtggcaaaa ggcatgggga tgactgagtt ccaatccatt ttttccgttg
     8221 aattgcccat tgctatgcca acgatcatgg ctggtattcg tttagctgcc gtttacgtga
     8281 ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg tttaggcgat ttgattttta
     8341 gtggactgaa taattaccaa ccagatctca ttttggcagg tacgatccct gtaacaattt
     8401 tagcactatt ggctgattgg cttctaggtc tcttggaaca ccggctgaca ccgatcgcac
     8461 ttagagagga gaatgaagaa tgaaacgtat actaaaaatg accatggttc ttttctctct
     8521 tcttatactt gcgagttgtt ccttccctgg tcttgccagc aatacagatg aagacaccat
     8581 ttcgatcaca ggtggaatca cttcagaagc acagatctta gcaagtctag ttgccggaat
     8641 ggttgaacat tatacagata aaaataccgc aatcatcaac aatctggcta ctacaactat
     8701 caaccatcaa gcgatgatga acggagatgc ttcaatttca gcggctcgct acacaggaac
     8761 agatttaacg actacattga accttcctcc tgaaaaagat ccgaaaaaag cgtttgctac
     8821 cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg ttcccgtcct atggttttga
     8881 aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa aaataccatc tatctaaagt
     8941 cagcgatctg aaaaatgtag cggatgaatt agtagctggt gtagatactt cttggatcaa
     9001 tcgtaaagga gacggttatg acggcttcca agaaacttat ggcttctcct tcaattcgat
     9061 attacctatg cagattggac ttgtttatga cgcagtcgaa gcaggaaaaa tggatattgt
     9121 tcttggctac tctacggatg gacggatcgc cagttatgat ctagtcatgt tggaagatga
     9181 tcgtcaattc ttcccaccat atgatgctgc tccagttgta gacaacaaga tattagctga
     9241 tacgcctcat ttaaaagaag ccttaaataa gttaggtcat accatcagta cggaaaaaat
     9301 gcaggaattg aactacgaag cagataataa tctagtggaa ccatcagtag tagcacaacg
     9361 tttcttacaa gaaaaccact attttgaagg gaagtgaagc aattggataa aatgaatgtc
     9421 tttcaacaat ttttctatta tttccaggaa aatggctcct atatctttgc acaatttatc
     9481 cgtcacttcc tgatttctat ctatggtgtc ctctttgcag cagttgtcgg gattccggtt
     9541 gggatcatga tttctaggag acgtaaatta gcaaactggg tgattcgttt ggcgaatatc
     9601 atccagacga tcccttctct agccatgatc tccattttaa tcattggatt aggcctggga
     9661 gtcaatgtcg tgattgttac tgtatttttg tattccctct taccgatcat caagaatacg
     9721 tataccggga tgatccaagt agacaaaaac atccttgatg tcggcaaagg catggggatg
     9781 actgctagac aacgactatt catggtagag ttaccactct ctgtttctgt tatcatggca
     9841 ggcataagaa acgcgttagt tgttgccatc gggataaccg cgatcggtgc attcgtcggt
     9901 gctggtggtc ttggagatat cattatccga ggaaccaatg caacagaggg cacatcgatt
     9961 attttagcag gtgcattgcc aactgcatta atggcgatca tcacggactg gcttttaggt
    10021 attttggaaa gacggctaga tccagccagc cgtacatcac gctgatgtta ttgtaaaaaa
    10081 caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt ttagttctgt catttttaag
    10141 gcaataatcc actctgattc ttccctcctt atgggcaaag agttgctttt cttagctatt
    10201 tatccattcc gtatactcca acttcatact gccaacgggt tcatatctgc atccaccgac
    10261 taagtttttt tcttatccgc taaggcattt tgccaatcca ttttcccatc actcgtcatg
    10321 gaattcatac cacatgatct ctaaatactc gtctgcttgt tctttgaatt gggcatcaat
    10381 tcttacatcc tttggcgtgg ctttcattta agccttttcc atcagtcctc gattgttcga
    10441 tgccttcagc tgcatcatca aaactggtga ctgccgtatc ggtaacttcg atcatctttt
    10501 atacaatcgg gaacttgcca tcctcatcct tggctaaaaa catatctagc cattccagct
    10561 tctcgccatg ggaggtttgc ggttcctaaa accggtacac gattcttatg gactgcatcc
    10621 gtcgcaacta gacttggcga tacgatgatc cttcaccaga agagcacccc cgtaaaccaa
    10681 ttgatcgata tattgccaat aagaaaagac attggcgtaa aagatatcga tccctcgtga
    10741 tgcattgccg ttgatactgc tattcatggc cgaaagcgcc acgactttaa cttcttccgt
    10801 ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc cgctctgcta aaggaacgac
    10861 actggtattg tatttcgtat ctgtatcttt tccaaaagac catgccaacg aatcctctgg
    10921 gaaccaataa gacgactccg gttgattgtc cattgctttt ttcaacatac ttttggctgt
    10981 ttctcgataa acgacatttc caattgtctt cgcctcactt tggcgataat cgatggtcgt
    11041 tgccactgcc aacactaaca acgatgctcc aattttactg attttccttg tcacacttct
    11101 tcctccttgt tttacataaa cccgtcaaca ttccattcct aaactcaaac aatgaccctt
    11161 caaaggtgga gatataggaa caaattaaag aaatcatgca ataaatccaa gaaggttggt
    11221 tttagaacaa aaaaagagtc tcctttttgg ggagactctt tgttaactat ctaaaaaggt
    11281 tggctcttct cacttcactc ataatgtgtc caggcagaat aaatttctac aactttattt
    11341 tcctcatcaa ctttgtaaac cactcgatgc tgtctattca gcctgcgtga gtatctgcct
    11401 tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca tatatggatc ttccttcaat
    11461 gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt gcttgatttt tcttaaatcc
    11521 actttcgcag agtttttgat tgcgactgta tagttactca tcaaagagct tcccaatcaa
    11581 tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg gtctcgtact ttatctaaag
    11641 tacctgtttg ctcaagaaaa agggtttctt gaatcgctct ccagtcttcc agcccaatca
    11701 atacagcatt gtttccacta cgatcactaa taatctcgat ttcagtatga ttttcattta
    11761 cctctttcaa taactgataa aaatttttac gagcagtgct aggattcatc gttgtattag
    11821 ccattgtctc tcctcctttt tctgatataa ttatagcgta cttgattaag tacgtcaaaa
    11881 aaatacaata aagcacttaa attcagcaaa cttttcctct tttttacata gagaaaaaga
    11941 ccccttttta gggaggatct ttgtccacgc tcacttctta ctcctttacg actagcggta
    12001 gccaaacgta aagtaattgg taacaacgta tcatctttag caaaggtcga atccaaagcc
    12061 tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc gttgcagtgt gacttgattg
    12121 atatcttgaa taacctgagg atacgcacga aagatttgta acggttattc aaaactgatt
    12181 ttggccgtca agtcttctac tgcctcaaaa atgattgcct ctttcgtctc ctgtcattca
    12241 tgttcactat cgatttgata cttgcttaga ccgttgggtt tgtgaatcac caattaaaaa
    12301 tggcactgag ttatggtaac aaaaaatggt cacttgacga ggctgagatt cctacttatg
    12361 aagacacatg gcacttaaga gtcgaggtaa aggaagaagc tgaaaatata ggaggctgta
    12421 cattatttgg caaaggattc ggaaattatg atcaagacat caaaatcaaa atgttttaca
    12481 gctaaatagg aagaataata acatttcata cgctgttccc atctttaatg gcttatagag
    12541 ggcaccttta gctgagaata agacaaaaca gaccctctag cttcaattaa gagctagagg
    12601 gtctgttttt gattttaaaa cttggtttat ttcttcaaat ggtagctata ggtgctgaca
    12661 acgatatttt cacgccaaga caatcgcaat cttaaccgca aacttgtttg gttatggaga
    12721 atattctgcc agcgtctgtt tggtacaaat tgaggaatca acacagtagt tgtatagttt
    12781 tgtttcgctg catttttact tacgatatct acataacgga taattggatt ttcaatcgag
    12841 cgataagaag agtggacgat tgaaaaacgg acatctggga aatgcttttt aaattctgct
    12901 tcgatttctt tttctttttc gacatcttcg tctagcgaaa catgcatcgc aacgacataa
    12961 tcaccgatcg aacgtgcgta attcaacgca ccgatattta cccgtgtcac attcccgact
    13021 aatacaagaa cggtattccc gtcataatca tgtagttttg catcattttc taaacgaagc
    13081 tgttctgcta cttttttata atgatcatgg atcttataaa aaatgaacat aacgattggc
    13141 ataatgatga agaaaggcca aatgtcccct aaccgataga caaacaaaat cgcaatgatt
    13201 gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt tgcttaacca tctttttcct
    13261 tcttttttcc atttgacgac catccctgtt tgagataaag caaatggaat gaacacgccg
    13321 atagagtata acggaatcaa gcgttctgtt gagccatgga aaataaacag caatacgatc
    13381 gaacctagcg ctagtgtgat aatcccattg gaatagccta gtcgatcccc acgatcttga
    13441 tacatgtgag gcatgaattt atcttttgcc agattgtatg caagaactgg aaaagcagaa
    13501 aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag caaattgaag gacgtaatac
    13561 aaaataccat gtccaaagac agctttacca atttgagaca aaacagtgac ttctttttca
    13621 ggaacgatcc cataccaata gttgataaat gtgatcccca cgaagaagaa acctaagatc
    13681 aatgccatca tcgtcaatgt agcagcggca ttttttgctc ttggcttctt aaagaatggt
    13741 acagcattac taatcgcctc caccccagtt aaagaggaag aaccagaaga aaatgctcgt
    13801 aaaatcaaag cgatagaaac acccggcaca gccgctcctg gaagtgcagt agcttgcaat
    13861 ggcacagcac ctgtcacgat attatataaa ccaacaacga tcagtaacga gatcacaagg
    13921 ataaatgtgt acaccggaaa taataggaac gatgcagatt ccctcagccc acgaagattc
    13981 agcatcatca gtagcaaaac gattgtcaca gaaatagcga cttgatgacc gtagagtgct
    14041 ggcaccgctg atgtgatcgc ttcagcacct gctgagacag atactgctac tgtcaacatg
    14101 tagtcgatca ataaagagcc accggaaatc aagccagcat tctttccaag attctcactg
    14161 ctgacgacat atgctccccc gccatgagga taagcatgga tgatttgacg atatgatagc
    14221 gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg gtagcgaata ccagatggct
    14281 gcagcagaca aagcgactaa aacgacaacg atttgttccg taccatatgc aatagatgat
    14341 aacgcatcag acgacaataa tgccaaagca gcaaatcggg ttaatttgtg ctcatcattt
    14401 tcagcagatt ttaaaggctt acctactaac aaccttttta agtaatccaa tttctaaact
    14461 cctttacaca caattttact tcaagtttta tagtcgaaaa tttatagtaa acaagatgtt
    14521 atagtacgct cactgacctt aaaagtcaat aaactttaag aaaataattg gaaaaaaaca
    14581 gaaaaatatc aagcgctttt tcaaacgctt taatacagca aaaatctaac atttttttat
    14641 gggctttttc tttgaattct ataataaaag gatagaactt gacttagaaa atcaagttct
    14701 atcctttttg atatcttaac ttttggaaga gagatgtaag gctgctttct acccatcatt
    14761 cttactactc gaagcatagc gcagttgtca caacctcttg ataaacggtg tttaaaagct
    14821 tgcatctgcg gtaataagct caaccaaatt aaaaacataa gaagctgttt ttatttggtc
    14881 gttcttatta cttgatgcag agcagctttt ttacaacctc ttggta
//
LOCUS       DACENJ010000053        14915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.053, whole genome shotgun sequence.
ACCESSION   DACENJ010000053 DACENJ010000000
VERSION     DACENJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.053"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(151..2148)
                     /gene="tkt"
                     /locus_tag="IW748_001182"
     CDS             complement(151..2148)
                     /gene="tkt"
                     /locus_tag="IW748_001182"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1104101.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2250..2501)
                     /locus_tag="IW748_001183"
     CDS             complement(2250..2501)
                     /locus_tag="IW748_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1104102.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2648..3271
                     /gene="lexA"
                     /locus_tag="IW748_001184"
     CDS             2648..3271
                     /gene="lexA"
                     /locus_tag="IW748_001184"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1104103.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3451..3669
                     /locus_tag="IW748_001185"
     CDS             3451..3669
                     /locus_tag="IW748_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104104.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3958..4470)
                     /locus_tag="IW748_001186"
     CDS             complement(3958..4470)
                     /locus_tag="IW748_001186"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1104105.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4484..6781)
                     /gene="recJ"
                     /locus_tag="IW748_001187"
     CDS             complement(4484..6781)
                     /gene="recJ"
                     /locus_tag="IW748_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1104106.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6923..7357)
                     /locus_tag="IW748_001188"
     CDS             complement(6923..7357)
                     /locus_tag="IW748_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1104107.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7367..8155)
                     /locus_tag="IW748_001189"
     CDS             complement(7367..8155)
                     /locus_tag="IW748_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1104108.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8152..9093)
                     /locus_tag="IW748_001190"
     CDS             complement(8152..9093)
                     /locus_tag="IW748_001190"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1104109.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9270..9857)
                     /locus_tag="IW748_001191"
     CDS             complement(9270..9857)
                     /locus_tag="IW748_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1104110.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10078..11391)
                     /gene="obgE"
                     /locus_tag="IW748_001192"
     CDS             complement(10078..11391)
                     /gene="obgE"
                     /locus_tag="IW748_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1104111.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11679..12689
                     /gene="gap"
                     /locus_tag="IW748_001193"
     CDS             11679..12689
                     /gene="gap"
                     /locus_tag="IW748_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1104112.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12774..13709)
                     /locus_tag="IW748_001194"
     CDS             complement(12774..13709)
                     /locus_tag="IW748_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1104113.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13895..14854
                     /locus_tag="IW748_001195"
     CDS             13895..14854
                     /locus_tag="IW748_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ1104114.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         4215 a         3292 c         2628 g         4780 t
ORIGIN      
        1 caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc gaaaacgaaa
       61 agtaatattg atttattctt ctaacacata aagaacagct gtctccaact tgcagacagc
      121 tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg tattcacgac
      181 attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag atgcaccgaa
      241 atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc caaatgatgc
      301 agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt ctttatattc
      361 agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa catctttgcc
      421 tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac ctgttgcaat
      481 caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc cttttttcac
      541 catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac gactcaatac
      601 aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac gtgtttcatt
      661 tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg ctagttgttc
      721 aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg ttaagacgta
      781 aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat aatcgacaaa
      841 gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac cattcatagc
      901 cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat aatgttcagg
      961 agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt ccgctgaacc
     1021 accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac ttgaaacacg
     1081 gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt tttctggtag
     1141 tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt ttttataatt
     1201 ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca tcgtttgatg
     1261 gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac cataaacttc
     1321 tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag atgttccttc
     1381 tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt tgtctgtttc
     1441 agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt ctttcactaa
     1501 gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga aagcttttga
     1561 agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt tccctagttt
     1621 catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt ctccgtcacc
     1681 gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat acgttgctgc
     1741 aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc ctaatggccc
     1801 tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg ttttagaatc
     1861 ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta aatgaagcaa
     1921 gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc ggtcagccca
     1981 gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat atgccatagg
     2041 tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg cttcaatact
     2101 taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa taacccctcc
     2161 tattaattta ggtttttttt acctttattt cttacacttt cattctataa gatgtcacac
     2221 gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt gtgtaagcct
     2281 tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc aggatcgacg
     2341 actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa atactctttg
     2401 cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt ctttttacgt
     2461 gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc tccttttact
     2521 ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa taaaatacga
     2581 acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt gaaagaaggt
     2641 gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat atatccatga
     2701 acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag ctgttcagct
     2761 ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag ggtttatcca
     2821 aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag aaaagattgg
     2881 gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac ccattttagc
     2941 agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg aagaaggcag
     3001 tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat tggatggtga
     3061 caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca ttgcaatgac
     3121 tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt tccgtctgca
     3181 gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat taggacgagt
     3241 agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa aactttttaa
     3301 aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct tctttcaaat
     3361 ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata tacgaacaat
     3421 tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct tggcttagta
     3481 ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc agctttactt
     3541 gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa gtaccgtcaa
     3601 tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta cagatccagg
     3661 caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa cttttacggg
     3721 ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta aagccagttt
     3781 ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg gtctttgctt
     3841 gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct gacttttgag
     3901 ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt tttctgctta
     3961 atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat ggagatccat
     4021 caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga tcagttcgat
     4081 cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca cacgttgacc
     4141 tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta atccataagt
     4201 cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa aaccgactcc
     4261 taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc caactaccat
     4321 atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc gataagcgtc
     4381 accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg gatagtcggg
     4441 aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt gttaaccatt
     4501 tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt ttattgagcc
     4561 gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg actttttgta
     4621 aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc ataaaaatta
     4681 ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga cgtatatcaa
     4741 ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt gtgcctaccc
     4801 cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg aatgaataag
     4861 accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca actagagagt
     4921 ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa acaatcgcag
     4981 cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct aaataaaggc
     5041 taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca aatacttgca
     5101 gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg ttccattcat
     5161 tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct tcttgagtac
     5221 caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca aatttcagat
     5281 gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga aataaaaact
     5341 gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca attcgctcta
     5401 ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct ctcatcaagt
     5461 caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg acattttcac
     5521 ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat aaatctcgca
     5581 tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat cctttagctg
     5641 atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc attattttcc
     5701 cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga tggatatgat
     5761 tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt gcttttcttt
     5821 cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca tcaaacgtcg
     5881 caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt agtctgggag
     5941 caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg ctttcttcaa
     6001 atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct aatgaaacaa
     6061 ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc gctactaaat
     6121 ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat gctactccga
     6181 ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga acaatcgcat
     6241 aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg tctacaccag
     6301 cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca gtgatgatca
     6361 actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca tacccatgct
     6421 caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt gtttctttca
     6481 ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg attttttctc
     6541 cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt atcagatacg
     6601 gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt tcctcatggt
     6661 aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca cggatcattt
     6721 ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt gcttgtctca
     6781 ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa agcagaaaga
     6841 aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga tcttttgtac
     6901 gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg ggcgattgag
     6961 ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct tgtaactccg
     7021 cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc tgttcttctt
     7081 ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc tgagtcagct
     7141 gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat acaatcagtg
     7201 caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag gaagtgaagc
     7261 caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata atcaaaacta
     7321 aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac tttttgttaa
     7381 aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat tttgatgcaa
     7441 cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg cggacgatca
     7501 tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa tagcttccgc
     7561 ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact gtcgtaacag
     7621 ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg actgcaaatt
     7681 tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata gaggcgatat
     7741 taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt gtcaaaacca
     7801 tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg aatgtaagga
     7861 agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc ccgacttctt
     7921 ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct accttgaaag
     7981 cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct acaaatgcca
     8041 ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa cagatttgtg
     8101 cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa ttcatgcctc
     8161 ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg tcgtgttttc
     8221 aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat atctcgcact
     8281 gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag cagcttgatg
     8341 agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa atgtgctttc
     8401 atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt ttctcgtatc
     8461 gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt ttccattgat
     8521 cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt acagtggtcc
     8581 agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat gatcatgttc
     8641 aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat aaacagaaaa
     8701 ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca gcggataact
     8761 taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc cttttggccc
     8821 gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga gcccgggaag
     8881 tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt ttctcggacg
     8941 aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca accaaacttc
     9001 attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt ttgcaggaac
     9061 acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct ttaagttcct
     9121 ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg ggttcaaatt
     9181 aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa acagcagcga
     9241 acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact ttcattgtcg
     9301 ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt cgcatgattt
     9361 tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa gcgagtaatt
     9421 tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc ttcatcagca
     9481 atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc gaacacggtc
     9541 tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc ggcaaaccat
     9601 cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat taatccaccc
     9661 gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa ttgatgtgtc
     9721 ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg aacagcatgt
     9781 aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa atttaaaatg
     9841 attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt attttacctt
     9901 taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg gctgtgacaa
     9961 aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga tcacttctgt
    10021 cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt tgcttgccta
    10081 ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt tcgcaccgcg
    10141 tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac gcatcactgt
    10201 ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac cagataagat
    10261 ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat tgaatccata
    10321 gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg ttacttctaa
    10381 caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc cagaaattgg
    10441 aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct gttctttgaa
    10501 tttcactaga ttttccggtg catccggcat atccattttg tttgccacga tgatttgcgg
    10561 acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa gatagtcttc
    10621 ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta ctcgtgttct
    10681 ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc cttcaatcaa
    10741 gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca ttcctagatt
    10801 aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg aaataacgga
    10861 tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg ccaatacttt
    10921 caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa tttccggtgc
    10981 aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac cttttgccac
    11041 aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct ccgcatctcg
    11101 gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac cgtgcatccc
    11161 tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc gaaaatccat
    11221 caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac caccgtctcc
    11281 gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca ttccgtctcc
    11341 gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca taattaagtc
    11401 ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca aaagtcttac
    11461 ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt gtttaccttc
    11521 ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag tataattttt
    11581 tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa ttgaggatgc
    11641 cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa gtaggtatta
    11701 acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc tcagatgata
    11761 ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa ttattgcaat
    11821 ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac tcaatcgtag
    11881 tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc ccttgggcaa
    11941 aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct gaagaaaaag
    12001 caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca gctggtgcta
    12061 tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac aaaatcatct
    12121 cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta aataatgaat
    12181 ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca caaatgctct
    12241 tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct gacaatacta
    12301 tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag ctacaaggaa
    12361 aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact gaattggttt
    12421 cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa aaacacacag
    12481 ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat gtcattggaa
    12541 caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct ggtgatttcc
    12601 aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc caaatgattc
    12661 gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa agaagccttt
    12721 gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact cgttcaatga
    12781 acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc tgtcttgtat
    12841 gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt cgttgcacga
    12901 tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg tataaagagc
    12961 attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa aaagtagcca
    13021 gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg aagactaaac
    13081 atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca atcttcaatg
    13141 attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt tgctgctccc
    13201 atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag cactttgttt
    13261 atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc cccttcgctt
    13321 tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg aaacatcttt
    13381 gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag agctgttttt
    13441 tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc ttttgaagtg
    13501 atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc cgtttctttg
    13561 gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga aggcacaaca
    13621 gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc taaagcgacg
    13681 cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc ttttttcata
    13741 taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt ttataacccg
    13801 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
    13861 tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga
    13921 cgaactgaca atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa
    13981 ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca agcaaggcaa
    14041 gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg gtagaaaagt
    14101 cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc ttggttggac
    14161 gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga gaacacttta
    14221 tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag gtaaaagaaa
    14281 acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa tccatgatcg
    14341 acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg atacgatcgt
    14401 tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta aagtcattat
    14461 cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta atcaatggtt
    14521 ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa aagaattttc
    14581 taactggaaa gccattagta accaacatga tattgatata tattttgcgg accctggaac
    14641 accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta atggactgcc
    14701 gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca gcaatcaacg
    14761 taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat ttttgagcta
    14821 tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata aaaaaacaaa
    14881 ataaaaaagc agaaataact ggctatttct agcgg
//
LOCUS       DACENJ010000054        14810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.054, whole genome shotgun sequence.
ACCESSION   DACENJ010000054 DACENJ010000000
VERSION     DACENJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.054"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..1695)
                     /locus_tag="IW748_001196"
     CDS             complement(1..1695)
                     /locus_tag="IW748_001196"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1104115.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            complement(1752..1880)
                     /locus_tag="IW748_001197"
     CDS             complement(1752..1880)
                     /locus_tag="IW748_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1104116.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            1879..2127
                     /locus_tag="IW748_001198"
     CDS             1879..2127
                     /locus_tag="IW748_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104117.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2319..2684
                     /locus_tag="IW748_001199"
     CDS             2319..2684
                     /locus_tag="IW748_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1104118.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            2681..3046
                     /locus_tag="IW748_001200"
     CDS             2681..3046
                     /locus_tag="IW748_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1104119.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(3138..4565)
                     /locus_tag="IW748_001201"
     CDS             complement(3138..4565)
                     /locus_tag="IW748_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1104120.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            5035..6870
                     /gene="tdc"
                     /locus_tag="IW748_001202"
     CDS             5035..6870
                     /gene="tdc"
                     /locus_tag="IW748_001202"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1104121.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            7175..8653
                     /locus_tag="IW748_001203"
     CDS             7175..8653
                     /locus_tag="IW748_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1104122.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            8619..9950
                     /locus_tag="IW748_001204"
     CDS             8619..9950
                     /locus_tag="IW748_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1104123.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            10064..12616
                     /locus_tag="IW748_001205"
     CDS             10064..12616
                     /locus_tag="IW748_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1104124.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(12623..14062)
                     /locus_tag="IW748_001206"
     CDS             complement(12623..14062)
                     /locus_tag="IW748_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1104125.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            14408..14770
                     /locus_tag="IW748_001207"
     CDS             14408..14770
                     /locus_tag="IW748_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104126.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         4658 a         2477 c         3033 g         4642 t
ORIGIN      
        1 ttattcaaac attttttttg cggcacgagc tagagtagga atatctttgt cgtaacgcgg
       61 tgttttgaca agacgggaca taggaatacc cgcaaaatgg aaagcagcga tttctccaga
      121 acgtacagca ctttgttcag taaagatcat ctgataaggc tgctcgacaa attctcctgt
      181 aaaggccaga ttggtcgaat gtttaggaat gacttttggt cgatcgcttt tggcacgatt
      241 attgaacaac gcggaagcat aaggcatata gacagggata ttatttatta cgctgtcgaa
      301 aatttctgtt tctttttcac gaatattcac tggtccagga tctacttttg aaagttgtcc
      361 aatcagttct tctagcattt cttttccatt cattttaata tactgtttat ccacaaattc
      421 gccacgtcgt cttggataaa ggaaataacc ccaaataact gtttcatttg gtttctgaga
      481 cgtaaaatgc ggttgatgat gcacgacgat agacatattg acatcttttt ggcctaacgg
      541 cgtgatcggt gtggttgaaa taaatgaatt caaggcattt cctggttctt gagtcgtgat
      601 acgggtgatt tcattcaaca acaaatggtt tttcgtcgtc agggtaaagc tgacccattc
      661 gcttgcatct cgatcagcaa agaatttgtc aggatttccc aagttgtaga aacgttcact
      721 ggcttttttc cagagactag aagctgcacc ataatccata ttttctacag ctggtgtttc
      781 gtaatctcct aaagtagctg aatctgtaat ggacccatta gtgaaaataa ctgctgtatc
      841 ttcatcgacc acaacgtatt cttcttcttg agtcagtgta ttggtaattt tcaaaccagt
      901 cacagtgatt tcatcttgca tcggtgtttc tttgaattcc cagtcagtga cacggcggtt
      961 caaaatgatt ttgcatcctt gttctttcaa gtactcgatc aaaggaagca taatactttc
     1021 gtattgattg taacgtgttc gattgacgcc cactagatgt tcgatttgag tgaattcata
     1081 aatcatttga tgcatatagc gacgcaattc ttgagcagaa ctacgtgttc tgaaagcaaa
     1141 tgttgtttcc cacataaacc agaagtttgt ttcaaaaaag tgtggatcat ctttgaaata
     1201 ttcttcgata gtgatattgt ctagtttttc ttcttctgaa tcaggcatag caatcagttt
     1261 agtcagaaga atacggtctt tattattgaa tcccagtttt ccgccatcaa tgattccttt
     1321 gccaccttcc atcaaacgtg ctttgtcaaa ggttcggtgt tttgcatcaa aatctctcgt
     1381 atcttcttca gcagtcatcc ctggctcagt aacagaaggt attcgactta acagatccat
     1441 caaatctaca taggtacggt aattaagcat gcgaccgcct cgagcaatat agcctttttg
     1501 gttttctaat ggatggtttt tattccaata ttcatccact acagtgtcgg tcggtgcacc
     1561 atcatttgaa ccgtgatcat ccaatgaata aaaggtgatc tgatcccctt tccatttgcc
     1621 ttcttgaatc aaataaacag ccgctgccat attggcaata ccggcaccaa tcattattgc
     1681 tttacttttt ctcattgtga ttcctccaat gtcttccaag tatgtccgta gtgattcaac
     1741 taaataggat gctagattga ttcgaattca tcatataaat gactgtcatc ttcgaatagt
     1801 cctgtctttt ttcctagata tgcagcgcca agggcaagca aacctaaacc gatgccgact
     1861 ttgattaatt ttttactcat gaaaaacgcc tccttgtttt ctctttctat atccaaggat
     1921 agttcgtttt tattagaatg ccaaatgata ggacgagaag aaaaaggaaa ttttgacaaa
     1981 aaagaagaag tgtctgattt tcctgtaaat aaaaataggg taggtaagaa gaaaagattt
     2041 gaggaggtgt atggttctat ttttagaaat ttgggtacaa aaaaagcacc gagacagtgc
     2101 ttctttgaaa agctgatgac ggactaagcc ttattttaac ttgctacgtt gttttggata
     2161 cttccacgat ttatggatgg agggagaaaa atgggagagt gaaaaagaaa aatttaagcg
     2221 aaccgttatc attagaacaa cttttaaata gacgaacaag gtaactttct tttacaataa
     2281 aagaatagga acaaattaaa agaaagtagg tagcgtatat gtttcgctca aatttaattg
     2341 aatttctaga atggatcatt gctattttaa atatttgttc tatcggtatc ttaattgtcg
     2401 ggatcgttct tgttctattc aaactttttg attggtctca tgtcaagaaa aactatgcag
     2461 aacgtaatac tcgtaatgca gatacaagaa aactgctagc aagttatatt ttgttaagct
     2521 tggaagtact agtcgttgct gatatcatcg agtcagttat caagccgact tggacagata
     2581 ttttgaagct tgcacttatt attatcattc gaacagtcat ttcttatttc ttgaatcagg
     2641 aaatagaaga caaagaaaaa caaagaagag gagaggtagg atgacgacag agttgatgca
     2701 gtttcttctt ccatatttcg aaaaacttgt actgggactt gatatctttg ctatctgttt
     2761 attggtttgg ggtgtttgtc tatcaatcaa ggattttttg aaatatttat ttgtccatac
     2821 aaaagtgcaa agcgaccgag tcagcgagaa caatgctatt aaaaagatgt tgggaggcta
     2881 tatacttcta agtttggaaa ttcttgtttc agctgggatc atcgaatcga ttatcaaacc
     2941 taccttgcag gatattttcc aactagcggc gttggtagtt atccgaacca tcatttctta
     3001 ttttttaaat aaagagatcg gacccgtaga tgtccaaaat aagtgaggtt gtgagcctga
     3061 cgtgtatcaa gagattgtta cagaaatggg catcttcaac aaataagagg ctgtgacatg
     3121 atttttgtca cagcccctta tttgttttgc acagctgaaa tgatagctcg atcgatcgta
     3181 ttgatcgaaa taggaataat attttcatat tggaccgcat ctggtgtata taatgtacgt
     3241 tcacataata cccggtttga ggactgctgc gcatacttct ttatatcaga tatacttgaa
     3301 taaattgttg gtgcaacttg aaaatcgaaa gtagttacag cttccaaggc ttcccgataa
     3361 atcagataat gaacatccga atttgtgacg atatagatag tcagatgttc ttttgtcaga
     3421 cggagaattc cgctcaattc gatcacaaaa gcccgcatgc aattaggatt caaccgtaga
     3481 tcataaggta actggctgga ccagctggaa aaaattgtct ggacttcttt atataaattt
     3541 gtctgttcct catttaaaag ataaaatttt tctggcatga ataattgata gttatcccaa
     3601 gccgaacgca tcagatgaat cagcgcacgt tcgaacggtt ggtttccgaa taattcttga
     3661 ttgaagtgag attcaaaggt agcaactagt tcttttattt cgggagtatt gtcaataaac
     3721 acttggtgta gctgttgaaa gtcttccgca atcaactgat aagaatggaa accataatta
     3781 caaagattga atagtgtcaa aataaaggcg aattcttctt ttttataata ggtttggaaa
     3841 tcagtgtttt cccatgctga ttcgacataa ttgtaaatag gacgtttttt gatttcttcg
     3901 ataaattttg aatctattgt cacagcacac acttgctggc gtaggaaact gatagcaaaa
     3961 ccaatcgata agatttcttt attttcttta tcataaaatc tgccactatt ttccgttacg
     4021 gattcaatga aacgatcggc acgttcaaaa aacgattcag gtagctccca agaattaaaa
     4081 ccgcctaaac gatacgacac attaagaagc agcagacgtc tttccatttc agtgaatcga
     4141 ggggagtagc ggtcgatttc gatcccgcag tcattaaaat aagctaatac ttgttttttt
     4201 aatgaataag ctttgctgac agagatgaat tcacattcag ttaattgaag ataatcaggt
     4261 tcatcttctt ccaaataaag cagacaagtt tttaaaaaca acgaattttg agacaattgc
     4321 tgcatgatct tgcatcgagg tatatgttct gaaaaatata aagtgtaacg gtccttttcc
     4381 cgatgtgtat aagcgatctg ttcttcaaat tcttttttta tgttattgat atcattgagt
     4441 aaagtggttt tgcaaacatg caggagattg gagagctttt ctagattgac ttctttattt
     4501 acatagaatt gcccacataa aaacagctgt cttaaaatat ttttctcaat atattcttca
     4561 atcatagtag agcctccgcc ttgtattttt ttgcacaata tgggaaatat tatgacatgt
     4621 tttctttttg aaatcatcag ttaatttctg aaaaagcata taaatgccgt taaaaaaggt
     4681 tttttatgtt ataaaatttg gaaaacagat aattctatta aaaaataaat tgcgaatttt
     4741 taacaatata aaaaaataaa aatatgttta gtgaatgaag gacagtcatt ttatttgttt
     4801 gttcgctttt tttaggttat ctattttaga agactgattt attgaaaaat tgttcgcttt
     4861 tttgcgttga attatcagaa aaatccgact ttatttattt ttgtttgtaa aaatctacgt
     4921 ggatttgatg ttataaaaaa aaaattctca tttttggcgc tatagtgaag aggtgttaca
     4981 gataaacaca tgtatctgca agacattgac caacaaaata tggagggaag aaaaatgaaa
     5041 gatatggata tcaaggccgt ctttataggc gacaaagctg aaaatggacc agtttataaa
     5101 atgttgttga acaagatggt agatgaacat cttggatgga gagaaaatta tattccttca
     5161 gatatgcctg caattagcga aggagataag ctcactccag attatctagc cacccgcgac
     5221 catatgatag aggtcttgga tgaagtcagt cagcgattac gcgcaggatc gatcccatgg
     5281 cattcagcag gccgttactg gggacaaatg aatgctgaaa cattgatgcc tgctttatta
     5341 gcttacaact atgcgatgct gtggaaccca aataatgttg cattggaatc ttctatggca
     5401 acttcgcaaa tggaagcaga agtaggacag gattttgctt cattattcaa tatgacagac
     5461 ggatggggac acattgcagc agatggttct attgctaatc ttgaaggatt atggtacgcc
     5521 cgttgcatca aatcgattcc tttagctgtc aaggaagtgc tgcctgaaaa agtgaaaaaa
     5581 atgtctgagt gggaactttt gaatttatct gttgaagaaa ttttggaaat gaccgaaagc
     5641 tttacggacg aagaaatgga tgaggtcaaa gcagcttctt cccgtagcgg aaaaaatatc
     5701 caaagattag ggaaatggct ggtccctcaa acaaaacact attcttggat gaaggcgtta
     5761 gatatttgcg gagtcggctt agatcagatg gttgctatcc cagttcaaga ggattatcga
     5821 atggatatta atgctttgga aaaaacaatt cgtgaattag ctggacaaaa aatcccaatt
     5881 ctaggtgtgg ttgctgtcgt aggaacgaca gaagaaggtc aagtagacag tgtagataaa
     5941 atcgtccaat taagagaaag gttaaaagat gaagggatct atttctattt acatgttgat
     6001 gccgcatatg gcggctatgc gcgttcactg tttctaaacg aagcaggaga atttgtgccg
     6061 tatgcttcct tagctgaatt ctttgaagaa catcatgttt tccaccactg cgtaacgatt
     6121 gacaaagagg tatatgaagg gttcagagct atttcagaag cggattctgt cacgatagat
     6181 cctcataaga tgggctatgt cccttatgct gctggaggaa tcgtaatcaa acataaaaat
     6241 atgagaaata tcatttctta tttcgcgcca tatgtgtttg aaaaatcagt aaaggctcca
     6301 gatatgttag gagcttatat tttggaagga tcaaaagctg gagcaaccgc cgccgcagtc
     6361 tggacagcac atcgtgtatt gccattgaat gtgaccggat atggtcaatt gattggggct
     6421 tcaatcgagg cagctcaaag attcagagaa tttctcgatc atctgacttt tactgttaaa
     6481 ggaaaaacaa tcgaagtcta tccattgaat catcctgatt ttaatatggt caattgggta
     6541 ttcaaagaac aaggctgtac agatttgaac gctatcaatg aattaaatga aaaaatgttc
     6601 gatcggtctt cttatatgga tggcgatgtt tatggtgaac ggtttattac ttctcatact
     6661 acatttacac aagaagatta cggtgattct ccgattcgtt ttgttgaaag aatgggtcta
     6721 acgaaagaag aatggaaaaa agaacagaaa atcactctgc tacgtgcggc tatcatgact
     6781 ccttatttga atgatgatcg aattttcaat ttttatacaa aaaaaatcgc caaagcaatg
     6841 gaaaagaaac taaacgaaat catccaatag aactgatttt tgtgtgaaag aataacaaga
     6901 gacatatgga gaacagacaa ttatttgctt catcaaaaca attgtatttc cccctgtttt
     6961 tcatatattc ctcgccaaga tattcttttt tataaaacgt tctataagtt ggatcgttcg
     7021 tttcatgtat caaacccagt tcactaagat cgttcaatgg atgtttcggt gatactgggt
     7081 tttgtactga atcgatgaaa ccttgtcatt tctgttcttg aacaaaaata aaagaaaaca
     7141 aaaacatatg tcgctattga aagaaggaat aaagatgaaa gatactggtt catcaaagca
     7201 aatcacgacc atgaccttta tcggaatgac ctgcgccctt gtagcaagtg tacgaaatat
     7261 tccagatgta gcagcaacga gctggacgat gattttttac atgctggttg cagtattatt
     7321 gtacgctttt ccaatctcat tgatctctgg tgaatttgct ggaatgtttc cccaaaaagg
     7381 tggtccagaa ctttgggtat cgaatgctct aggaaaaaaa tgggggttcg ttgtctcatg
     7441 gctgttatgg gtccaaatgt ttcctgggat ggtgatggtc gcttcagcac tcgctccgtt
     7501 gtttggtaat attatcgata atgtgccgct tggtttgaac agcaaattta cattagttgt
     7561 cattttagtc gtttattgga tcatcacttt tttgaatttg aaatttgata tggcaaaaat
     7621 cggcggaaaa gtcggtgttt ggttaggatt gtacgtgcct ttgacgatga tgctcttatt
     7681 agggtttgct gcgtggatca aagttgggat cgtaccaacc ggaacattag gcagtttttc
     7741 atgggacaaa ttaataccag atactacgac tgcatcctca tttgtttatt ttgcgccgat
     7801 catgtttatt tttactggta tcgaaatgtc ctctgtgtat atcactcgat tggaaaatcc
     7861 agttaaaacg tatattcgcg gagtatttgc cgcgttaatt tttatatttt tcgtcaatat
     7921 tttgaatgct ttagttgtag caaatgtcgt accaaaagga caaatggaat tgaataatat
     7981 cgcgcaatct atttcgattt attgccaaat tcttggtttg ccacatctta ttgtgaatct
     8041 gtttagttta cttgtcttca ttggtgtagc agtccaatta tctgcttggg cctcaggacc
     8101 agcaaagaca gtgacagaaa gtgctagaaa aggtgcgtat ccaccaaaat tcaatttttg
     8161 gaagacgaat caatttgacg tatccaaatc cgttattttg actcagtctg tcattatctc
     8221 aatctttgca cttttttatt tgctgattcc tggcgtcaat caagcatttt tgatgctcgt
     8281 aaattcaact actgttatct actgtatcgt ctatgtcatc atggggatcg cagttttgag
     8341 attgcgttat acacatgcaa agttgaatcg accatttaga attggaaaaa aagagaaaaa
     8401 gagcaatctt ggagtttgga ctgtcgttat tgttttattt gcggcaatcg gattttctgt
     8461 cggactgacg atgaaagctg gaacatggat caatctaatt gcagtcacag ccatcagtgt
     8521 cattttgttt gtagtaccac tctgtattga aaaaataaag aaaccttctt gggaacagga
     8581 agtcgccatg ctgcttgaaa aagaaaaagg tgaacttgat ggaaaaaccg gcaatgaaac
     8641 ggactctaac taatcgaaat atccagttaa tcgcattagg tggcgcaatt ggaacaggat
     8701 tatttttagg ttcagcaaag gcaattgcat cagcagggcc agctgttatt ctcgtttatt
     8761 tgatatgcgg tttggctatt tatggtttga tgcgggccat gggtgattta tttttagcaa
     8821 atagtacatt taactctatc agtgatttcg ttgactatta tttaggcaaa aagtgggcat
     8881 tttttgtcaa ttggacttat tggctttgct ggcttggagc tggacttgcg gaattaacag
     8941 caataggatt gtatatccat ttctggtttg tacatgtacc agctttgata agcggaggta
     9001 gcgcactaat gatcttaatg gcagtcaacc ttgttgcagt caagtggttt ggtgaattgg
     9061 aatctttttt ctcaatcatc aagatcttgg ggattttcct ttttatctta ttgggtatag
     9121 gattgagtgt ctcagatttt catacccagt ctatcggaat caaagaaaat tttggacaga
     9181 tcgatgaatt actaccgttt ggctttgctg gaatggtagt ctcatttcct atggttttat
     9241 tcagttttgc tggaattgaa atgatcggct taaccgttgg cgagacaagt gatccgcaaa
     9301 agaatttgaa aaaagcaatc aactgtctgc cttggcgtat tctctttttc tatataggaa
     9361 cgattttgtc gattttattt gttgttccat ggcattgttt agatttaaaa gaaagtcctt
     9421 tagtgactat gctgcaagtg atccattgcc aaggcggagc agcaattgtc aatctaatga
     9481 ttttggttgc ttctttgtcc tcagcaaata gtgcagtcta cagtactagc aggattttat
     9541 atcaaacagc aaaagaaaaa agtttgcccc attggttttg tgcgctttct aagaaaggcg
     9601 tacctttaca cggtatttta attacaggaa tgttattttt tgccggtctt tgtctacatt
     9661 tttttgtagc tgatagtcga ttgttttttc agcttttggc aggcatgaca acgagttgtt
     9721 ttctatttgt gtggtcttcg atcttgtgtg cacatgttca atttattaaa gaaaagaaac
     9781 ggaacactac acaaagatat aaagatcttg ctttgttggt gttttttact tcaattgctt
     9841 tcagcttgtt attcgaaagc acgacacgct ttattccttt tttattggtt atctggtttg
     9901 tattcttaaa ttttgtatat aaaagaataa gcaatagaaa tatcagttag gcccagatac
     9961 attttgcaaa acgaattatt tcagaccccc ctgaaataat cgatacgcct gaaaaaactt
    10021 ccctgcgtat aataacagta tcaattaaac aggaggggat aggatgaact ggtacagatt
    10081 gacggtagaa gatgtattga aaaactatca aacatcaaca gaaggcttgt cgcaagaaga
    10141 acgacagaaa cgcctgactg atcaaggata taatgaaata aaagcaaggc agcagacaaa
    10201 acgatggaaa aaaatagcaa agcattttac tgatctgctg atgatcgtgt tgatcattgc
    10261 ctcttttttg aaatttgctt ctagtgaata tattgaagga agtattattt tattcgtagt
    10321 gatcgtcaat ggactcgtta gttactggca agaaagaaaa gcggaagaat cattgaatgg
    10381 tttgaagcag ctgatgggac aagaagcgat agtactatca aacgggatgc aagaaaaaat
    10441 ccctgcgaaa gaactggtaa aaggagatgt cattgtctta cgtgccggag atgtcgttcc
    10501 agcagatatc cgattaatcg aagcacatga tttattagtg gaagaatcga ttttgacggg
    10561 agaatcagaa gcatctgaaa aaagtcatgc cgttttgaca gaagaagaat cgatcggcga
    10621 ccagaaaaat atgggttttt caggaacgct tgtacaatct ggttctgcag tcggtgtcgt
    10681 tgtagaaaca ggaaacacga cagaaatcgg taaaatcaat caagcattgc aatcggtcga
    10741 acaacaaacg acgcctctga ttaaaaaaat caaccgatta aacaaacaaa tttttcaagg
    10801 cattctttgt ttgagcctat tcttagttat ttttacgaca tttcgatatg gtatggattg
    10861 gcatatcctt ctttcggcta cgattgcttt ggtggtgtca atggtacccg aaggattgcc
    10921 agctgttttg accatgattc tatccgttgg agtacatgaa atggcaaaag aaaaggcgat
    10981 catcaaagga cttccttcgg tagaaacatt aggttctatg acagtgatct gttcagataa
    11041 gacaggaaca ttgacaaaaa atgaaatgac agttgtggat gtagctgcaa aagaagaagc
    11101 ctgcgtgttg tccattatga aaaactgtca ggaattaaaa acaaaagaag aacagaaaac
    11161 agaaaattta agtggcaatc cgacagaagt tgcgctgatt cgacacacag aaacagccaa
    11221 tctacctttg aaaaaaatag aagcaaagat tccgttcagt tctgaataca aatatatggc
    11281 aacgatgcat gcggaggaaa aaggagcggt gatttacgtc aaaggtgcac cagaagtatt
    11341 atttgctaaa tcaactcttt cttctgcaga acaagaagca tggagtcaga cagctgcaga
    11401 atttgcgaga aaaggtcagc gtgttttagg atttgcttat aaaaaagtag attcgaaaca
    11461 ggaactcacg catgagactt taacccaatt aacttttgca gggatcgccg gattgatcga
    11521 tccgccaaaa gaaagtgcag tcaaagctgt aaaggaatgc caacaagcag ggatctcagt
    11581 gaaaatgatt acaggggatc acaaagacac agcaaaagcg attgcggaac aaattggctt
    11641 aaaacatact gccaaagttt tagaagggat cgatttggat cttatgtctg atgaagagtt
    11701 gattcagcaa gtgccaatag tcgatgtctt tgctcgaacg acaccagaac ataaactgcg
    11761 aattgttaaa gctttgcaga aaaatggaga aatcgtcgga atgaccggag acggcgtaaa
    11821 tgatgcacct gctttgaaaa gatccgatgt aggaattgct atgggaatca aaggtagtga
    11881 agtaagcaaa caggcagctg atatggtttt aggagatgat aatttccata cgattgcaaa
    11941 agccgtaaaa gagggccggc ggattttgga taatttgcaa aaaaccatca atttcttctt
    12001 gccaactgca ttagctcaag gattgatctt gatttgggca cttatgctga atcgcccgct
    12061 acctttgtcc ccagtacaga ttttatgggt caatatggta acgacaatca ctttatctta
    12121 tgcactaggg ttcgagtcgc cagatccaga aatcatgaag cgcccaccaa gagatccaaa
    12181 gcaaggaatc ctatctggtt atcatttgtt tcggatcatc tatgtctccc tactgatcat
    12241 ggttcctgcc tatttattgg ctatgcagtt tgaagggcaa agcttacagc aaaccattct
    12301 tctgcaaaat atcgtcttag ctcaagctgt ttacatgatc aattgcagag agttgtccaa
    12361 gccgtcattg aaccaaggtc ttttgcaaaa caaagcgctt tttctttcat taggtatcct
    12421 tgctttattg caagggatgg ttatttttct accagtcggc cagcagctga tcggtacagt
    12481 aagtttaacg atgacacaac agttattgat tggagcaaat gtgattcttc tattcttagt
    12541 tgtagaaata gaaaaaggga tcatgaacaa actgtttcgc aaaaaagaaa aaacagctag
    12601 agaatacagt cgctagccgt tactataaaa aagttttgaa agtgagttca gtcaaacgat
    12661 tgagctcatt tttcgttgga atagaagaaa tgtatacaat agggatatct agagaatcta
    12721 gagggaaatt ggaaatcaag atatctgttt tttcaaaaga aatagtggaa agatcggtta
    12781 gttcgatagg taagaagcag acacgttttc ctaagtaatc ttttaattcc tgctgcaaga
    12841 acgccttcca tgaaggttct ccttggaaaa taaaatagac attgaacaaa ttcggtttga
    12901 tagaaaaaat cgtttgttgg atcagtagag acaagagttg gagaaagtaa tttggttcat
    12961 tgatttctat ttcttttttt agatcgttca gtaaattctc tttctgttga tacaagtgtg
    13021 catattgctg atcatatgtt tctaaaaaac gatggtattg acggtccaat gcttcaatca
    13081 atatcggcga ttcatgatat tttaatatga ggagagccaa gttatcagtc agagatgttt
    13141 tttttagttg atcaagagac agctctgaag ctagttcctc tacaaacgtt tcgaggacct
    13201 ctaaaaatgg agaatgatgc aggcagcgag cggtttcttg tccatagtta ttgttgtaat
    13261 tccaactttc cagaaagcag aaagaagcaa aagaaagctc agtgtcttta agataaatac
    13321 cttccgttaa atacaatttt ttcaatgcag agtcatacag ggaggagagt gtgctgttgg
    13381 tatggataga agaatctaat cggcaattcg cttttatacg gatcgtattg ataaagaacc
    13441 aacatgttcc gaaaatctct tctgtttcaa ccgctagttc agtttgactg aggtttctta
    13501 aaaattgaaa ataatgggat tcatggatcg aataatcctc gaaaaaatag ttgttatgag
    13561 tgaaaggaat cagcaatcga tgataaaaag tacgaatcgc acgctctttt ccaagaatac
    13621 aagctgtata tttcgtcaaa tcgatctgca agtcgtaact tgctaaacgc tgattgattt
    13681 tagtcacatg tcttttcaat gtcccataag aaagcccatg gtcggctaga aaatcaggga
    13741 cagcaacttt ttttgtaaaa agcagatttt tcagcagttc gacactgact gattgtttga
    13801 aaaaaatctc ccaaaggtca tttgttaata aattttgctg gttttgcaaa ctgattcctg
    13861 cttgatcgct gatgatgtcc cagccttcgg gcagctgaga gcgaatagat tgaaggtcag
    13921 aaaaaatcgt tcgttcggtt gtccctatga tttcagctaa ttcatttacg gttatctttg
    13981 tatgattcag caattgttct actaaagaaa catgacgtcg aatatctttt tcggtaatga
    14041 tttttttcat tagtgaatac atcaatcaga ctcccttcta ttctaagaat acggagaaaa
    14101 taaacggtgg tcaagtttca actgattttt caatcattgt ttatttttct ggagcattta
    14161 ctgatgataa gaatttcatc atgaacttga aaaatgatac cactaaaatc aaaaaatagg
    14221 tgggcaaaca ataataaaca ttttaatagt agatggtagc aagaccaaca caggcggatt
    14281 gaacatttaa gagttgataa aaaagaaaaa tatcgaagta tttgattagc aactacttgt
    14341 attcattttt ctattcttac gtggtatctt tgaataaagc gaatcaataa gaaagagatg
    14401 gaagcagatg ctaacgaaaa aagaactaat cgatcacatc aatgaaacat acaatttgtc
    14461 tccagaatat atttttaaaa aatttcctga ttattgcgta ttcagacatc aaaaaagcaa
    14521 aaaatggttt ggggtcctga tgtctgttcc agaagaaaaa atctatgatg atgcaagtga
    14581 ccataggatc atcaatattc ttgatttgaa ggtcgatcct gaacttggcg gaattttgcg
    14641 aggaaaagag gggtattttc cagcctatca catgaataaa gaacattgga tctctattga
    14701 tctacgtaaa atagaaaagc tagatgaact agcagatttg attgatggaa gttttaaatc
    14761 aacgcaatga atgtctagga caatagagaa gaggttgtga cagaaatgtt
//
LOCUS       DACENJ010000055        13957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.055, whole genome shotgun sequence.
ACCESSION   DACENJ010000055 DACENJ010000000
VERSION     DACENJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.055"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(105..1562)
                     /gene="ascB"
                     /locus_tag="IW748_001208"
     CDS             complement(105..1562)
                     /gene="ascB"
                     /locus_tag="IW748_001208"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1104127.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1559..3454)
                     /locus_tag="IW748_001209"
     CDS             complement(1559..3454)
                     /locus_tag="IW748_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1104128.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3512..5287)
                     /locus_tag="IW748_001210"
     CDS             complement(3512..5287)
                     /locus_tag="IW748_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1104129.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(5337..6833)
                     /locus_tag="IW748_001211"
     CDS             complement(5337..6833)
                     /locus_tag="IW748_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1104130.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(7027..8649)
                     /locus_tag="IW748_001212"
     CDS             complement(7027..8649)
                     /locus_tag="IW748_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1104131.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(8977..10290)
                     /locus_tag="IW748_001213"
     CDS             complement(8977..10290)
                     /locus_tag="IW748_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1104132.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(10535..10759)
                     /locus_tag="IW748_001214"
     CDS             complement(10535..10759)
                     /locus_tag="IW748_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104133.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(10779..11147)
                     /locus_tag="IW748_001215"
     CDS             complement(10779..11147)
                     /locus_tag="IW748_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1104134.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(11344..12054)
                     /locus_tag="IW748_001216"
     CDS             complement(11344..12054)
                     /locus_tag="IW748_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104135.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(12072..12569)
                     /locus_tag="IW748_001217"
     CDS             complement(12072..12569)
                     /locus_tag="IW748_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1104136.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(12606..13058)
                     /locus_tag="IW748_001218"
     CDS             complement(12606..13058)
                     /locus_tag="IW748_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1104137.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(13193..13798)
                     /locus_tag="IW748_001219"
     CDS             complement(13193..13798)
                     /locus_tag="IW748_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1104138.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         4106 a         2910 c         2242 g         4699 t
ORIGIN      
        1 atgactatgg tgacttgtga tcgaggagtt ggcaggagaa tactgtttat tcgtaaagaa
       61 ggggctgtga caagactttt gtcacagtcc cttatttcct tttattatag gatttcgcca
      121 tttgattgga tgacttgtct gtaccaatca aatgattttt tctttaaccg tttctttgtg
      181 ccattccctt cgtcatcttg atcaacatag atatatccat aacgttttga catttgtgaa
      241 gtagaagcgc tgacgatatc aatgcagccc cacgacgtat aaccaaataa atctactcca
      301 tctttgatag cttccttcat ctgctcaata tgctgcttca aatagtcaat gcggtaatcg
      361 tcttgaacgg ttccatcttc ttctactttg tctatagctc cgagtccgtt ttcagctatg
      421 aacagtggtt tttgatacct atcatataat tgattcaaag caattctcaa acccttagga
      481 tcaatctgcc agccccattc acttgatgga agatacggat ttttaattcc tccagttatt
      541 aagtttcctt ggaccccttc ccaattttct ggatgagcac tgactgcact agacatgtag
      601 tagctaaaag aaacaaaatc aacagggtag gctgctaaga tttcttcatc acctacttct
      661 atttttaagt tgacaccttt ttcttcaaac attcgttgcg tatacgctgg ataatatcct
      721 cttgcttgaa catcactaaa gaacaaagaa gttctttgct gctcaattgt tttttgaata
      781 tcatccggat cacaagaata cggaacaaga agctgatagc tgatcataca accgacttgt
      841 gaacctttga taatttcatg acaagctttt gtagctaaag cacttgctat aaactgatgg
      901 tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt tttcttcaat tactcctacg
      961 ctaacaaaag ggtgatgttt tacacagttg atctcattga aagtcatcca atattttact
     1021 ttatttttat agcgtttgaa tactgtttca gcgaaacgaa cgtaaaactg aataagcttt
     1081 cgattaggcc atccaccata ttctgtaacc aaatataacg gcatttcata atgggaaaga
     1141 gaaacgactg gctcaatccc atattttaga cattcgtcaa acacttgatc ataaaaaaat
     1201 aatcccgctt cattgggtgt ttcttcatca ccatgaggaa aaattcttgt ccatgcgata
     1261 gacatccgat agcatttaaa acccatttca gcaaataaac ggatgtcttc tttaaagtga
     1321 tgatagaaat catttccgtg tcttttagga taataatact tgctgttggg atcctgcgcc
     1381 gcttttactt gctgatgcgt catcatatgc cattgatctg tccatttttc tttaggtaaa
     1441 tcgctgtcga atgtatacac atcagacaca gataatcctt tttcatctac atcccatgca
     1501 ccttcacatt gattggccgc tgtcgctccg ccccataaaa atccttctgg aaattttttc
     1561 atatcgatcc ccctaaactt gctagttttc ttgtttgatt atttttctta tgactagtaa
     1621 tgatattttt gaatctacta ctatttgtaa caataaccat tgtggtcgga tcatatccct
     1681 ctttgattaa ttcttgcaaa tctacttcca tcaataaatc gcctttacgt actttttttc
     1741 catcagatac tgcactttga aagtatttgc catttaaatt cactgtatca ataccgatat
     1801 ggatcagtat ctctgctcct tcttcactga ctaccccaat tgcatgtttc gttggaaaaa
     1861 ctgttgaaac ttccccgttt acagaactgt agatttttcc gtccgttgga atgatcgcaa
     1921 acccttctcc taaagatcca ctagcaaatg ttggatcgtt tacttcagat aaatcaatta
     1981 tttcacctgc tgcaggagta cttaacacta tatcctttga ggattcttct tcgatttcat
     2041 ccttgtagcc gaaaagaaaa gttagcatac aggaactgat catcgctaag aaataactag
     2101 caactagcca aacaaatcct tcaccaatat aaatcgggaa agacaaaata cttcttgtag
     2161 caactgccaa agcaccggct cctgcggcac cagctactcc accaaatata gctccacata
     2221 tacaagcaat gtaaaatggt cgtttatatt taagagtcac accataaaca gcaggttcag
     2281 tgattccaaa taatgctgtg ataaatgcag ataaagataa actttttact ttcttatttt
     2341 tcgttttcaa aaatacccca agtactgctc ctgcttgcgc agctacggca gtcatacatg
     2401 ctggacctag cgtatcacga ccgtagaaag aaagattgtt ggtcattact ggtacaagtc
     2461 cccaatgtaa cccaaaaatc actaacactt gccaaaatcc gccgataaca gcaccagata
     2521 aaacagggct aaagttataa acagctaaat atccagctgc aatcagctga cttgcataat
     2581 ctgcaattgg acctacaact aataaaataa ttgggaccat cacgatgata caaatgaatg
     2641 gtgtcaataa atttcgaata ctttcgtgca agcttttgcg aatcttaggc tcaatataag
     2701 aaagaaccca tacagctaaa attgctggaa taaccgtact tgtatacctt gctagaatca
     2761 cgggcatccc tagaaatcgc aaggttatgc tatcactata tgcactgaca atcgtaggat
     2821 aaacgagagc tgctgcaata accatagcga tgaaacgatc tgtattgaac ttttttgcag
     2881 cagtatatgc caaaaatata ggcaagaaat agaaaaatgc atcagctgct gcattcaaaa
     2941 tgatataagt tcctgattca gtagttaata aattcaaact cgtacataag gcgagtactc
     3001 ctttgatcaa accacttcct gccaagacgc ctaaagtcgg ggcaaaaaca gcggcgatca
     3061 tgtctataaa agagtttaaa atgccttttt tctcagttcc tctctttcca ctagatgcat
     3121 catcttctag tttcatttgt ttggataata cgtcatacac ttcatttact gcatttccta
     3181 ctacgacttg aaacattcct ccagcttcga ctacagtgat cacaccttct agttgctcta
     3241 ccttctcctt atctgccttc ttattatctt ttagggtaaa gcgtaacctt gttgcacaat
     3301 ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg gacaatatct ttcgctaatt
     3361 gtgaatagtc catttttact tcccccttgt taattaatgc cgtagcaatc cttcttacaa
     3421 gataagtata gttcttagaa aacggtttct ccattcaatt agtacccctt ctaatagcga
     3481 acgaaactgt acaatagtgt gtatggttta cttagccgag caaatcactc actgcttgaa
     3541 agaaaacaag tatatcgttt ctcttatagt caaaggagac aaaaaaaaca cgcttgatct
     3601 tcctttgctg atgtataagt ggtatctcaa gctgatactc tgtgattttt gtttctactt
     3661 ggcttttgat aagacagcac aacaaagtct cggcatttaa cggaagcata actaggtcag
     3721 aactaggaga gattagttgc tgaatggaat ctatcttttt atggattttt atttgatttt
     3781 cttctttgta gtaatgatca agcagttttg ttttcttttt ttcttcaact atggtcagct
     3841 tttcacgcaa aatctgataa atctcccaag attcaaactc atctagaaca ctaggtagat
     3901 aaatcaaatc atcccactca aaaataagct cttgctcagt agtcaaaatt gcatcatatt
     3961 ccgttatttt gtttgattga tttttgaaaa gatacactgg ttctatccgg cattctttga
     4021 tccacatgtt catactttgt tgaatctgtt tttttagtgt attcaaaata ctaattggct
     4081 ggttactaac aatcagtatc tccccttgtt ttcgaaaagg attcaacgct tcggcaagat
     4141 acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa ttgaaaatgt attgggaaga
     4201 aacgccgaat taaataaatt tctaaaggat aacgggagat gatcttttgt acaaaatgat
     4261 tcatggtatt atgtccggca tccatcctcg ttttcatttg tttgatatgg gttagcaaaa
     4321 attctgtttc atggaacaat aacgaaacag gaagatctaa aaaacgaata atcgtttgct
     4381 caaatgctct tagattcttc tctatttctt tgtttttttc atcattaatc atcaaataat
     4441 ttaactctaa tagtcgattt tcaattgctt gtttttcttg attggaaaaa gaatagggta
     4501 gttctgatga taactttttt gtgagggtct ctaccatcgg aataaataat gtatacctgt
     4561 ttgttttttc taaaagttct tctcttcgaa gaatggtcaa aacgagatac cgagaaaatc
     4621 gtatgaagtc ctctcctgaa acaatataat tatatgatat aagaacctct tttaatgtct
     4681 tttggacagt cggcatgata ttggaaaaaa ttgtactgat ttgttgaggc aaccttgcgt
     4741 gttggatcac cgtttctgtt ccaacaagag ataagaaaat tctttttgaa ttttcttcag
     4801 caatgatttt tagtcctttt gtcgacgaaa cttccagttc taatttagga ttgcgctcga
     4861 tccaacgcaa aatcgttttt atttgtaatg atacggctgt ttttgaaaga aagaaaatgt
     4921 ctgctatctt ttgcatactg acataccctt tttgaaacaa taagtacgtc acaatagttg
     4981 aagctcggaa atccatgctg gaatagattt tatgtttcgt cacctcttgt atgatttgct
     5041 gtttagtttc agattcaatt gattctaaaa aataacctga acgtttattt gactggataa
     5101 tgattttctt ctcgtagata gcaagcatat ttttgatttc agtttggatc gttttcttgt
     5161 tggtacctaa agcttcagct aattcttcgc ttgttatcca atgattctta tttcttaata
     5221 ctacaagaat ttggagttgt cgtttattta agtccatttg cttacacctc ctctttgtta
     5281 taaacataca ggaaaaacct ctcaaatgac aatagtcact tgcagaggtt taaaaatcat
     5341 ttgaagaaag atctatattc aaggttttta ccattagact ggataatttg tttataatat
     5401 tcaaaactat cttttttcaa tctcctaaag cttccgctcc catcattttg tctatccaca
     5461 taaataaagc catatcgctt cctcatttcc cctgttcctg ccgatacgat atcgattggt
     5521 ccccaccata agtaaccaat gatttggcaa ccatctttta ttgcctcagc aatttcctga
     5581 agatgtattt ttagatactc acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt
     5641 gcttcatcta gtccaatgcc attttcaact atgaataatg gtaattcata acggtctgtc
     5701 aattcattca tgacataacg aaaccctaga ggatcaattg gccaactcca aggttcggga
     5761 gatttttcct tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc tacgcgcatt
     5821 tctgcttctt ttttatagac agcgctacga taataactaa atgcaatata atctgccgtg
     5881 ttagcttgca agcactgttc gtctccatct aacattactg gtgcacagtc ttcttccttc
     5941 catgtcctgt aaatataagc tggataatgc cctcggcaca tgacatcgga gaaaaaccat
     6001 gaattgcgtt taaattgcag tgaaccaaat acattctctg gatcacagtc atatgcataa
     6061 gtcgctaatg cagaaaagct acacataata cccatcttag catttggatc gattttcttt
     6121 aataattgag tagcacgggc gttcgctaga aacatatgat gacaggcttg ataaatcact
     6181 ttttttgtta gtgtagcaat gggttcaagt ttatctctag cagggaatgt aaccaatcca
     6241 gcagctgcat aaggcatacg aaaagcatta ttgatctcat taaaggtcat ccaatatttc
     6301 actttccctt ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat
     6361 tttcgatttt gccacccccc atactctcct accaatgcta agggggtttc ataatgacta
     6421 agagtaatta ctggctcgat atcatatcga tttaatgtat tgattagtct gtcataaaat
     6481 acgagaccag cctcatttgg cttctgttcg tctcctcttg gaaatattcg tgcccatgag
     6541 atgcttgttc gaaaagcttt taaacctaac tccttcagta aacgaatatc ctcttcaaat
     6601 cgatgataaa aatcaatcgc ttcatgagaa agataatgga gtgattcgtc taaagcaatc
     6661 tcccaaatct ttttttcttc attccactga atactaggat gctggttcat gatcccgata
     6721 agcgtatctg tcacattagg taatttgccg tcttccaacc atgcaccttc acattgatta
     6781 gcagctactg ctccgcccca taaaaaattt tctggaaata tttcctgatc cataactatc
     6841 tctcctcgct atcaacaatt tcttacttga agtatagctc gattcatcaa aacaagatat
     6901 tctaactatt tcggcagaag agaagaaaaa aattgaatgc tactcttcat ttttaatatt
     6961 tatatataac aaatacataa gacgatcccc gaaagaaaac actcgttaac catctaagca
     7021 aacgatttat tttacttcca acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc
     7081 tggtaactgt ttcagaggat aatttgacac aataaccctt aaagcttcac gtggaatagt
     7141 gaacgtctgc ttttcactgg acatattaca aataacgagc cattgctggt cttcatatat
     7201 gcgagaatag gcaaagacat gctcaggcgt cttttccact aattcatagt ctccccagac
     7261 gatgatgtca tgttttctac gcaaatcgat caatttcttg tacatataaa aaacagaatc
     7321 ttgatctgct aacgcttctt ctgcattgat ttctttataa tttggattga ctcgaagcca
     7381 tggaattcca gtagtaaacc cagcatgttc acgggcgttc cattgcatag gcgtcctagc
     7441 attgtctctt cctttcgcct tgattgattc caagatttcc tcttttgtaa atccagaaga
     7501 aatcctttcg ttatacatat tgattgtctc aatatctttt gcttccgcga tatcatcgat
     7561 tgggcaattc gtcatgccta actcttctcc ttgatagata taaggggtgc ctttcatcat
     7621 atggagcaag atagcaaaca tcttggcact ttcttttcga tacacatcgt catttcccca
     7681 acgagaaaca attcgcggaa gatcgtgatt attccaaaat aaactattcc atccttcatc
     7741 acctaaagat gtttgccatt tggataatac ttttttcagt tcagagatac tcaagggttt
     7801 caaatcccat ttgtctttgc cttcttgctg atctaatcct atgtgttcga attggaagac
     7861 catggataat tcatttcttt taggattgga ataaagttta gcgatttcgg tagttgctcc
     7921 ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa gttgcttgat tcatttcctg
     7981 aagatactca tgcagtttag gtccattact tgtgatttct ttatccggtt ctttgccaat
     8041 catatcgatc acgtccatac gaaagccccc aataccttta tcgatccaga aattcatcat
     8101 ttgataaatc tcttggcgaa cctgtttatt tttccaattc aaatcaggtt gacgcttgct
     8161 atacaagtgc agaaaatatt gccccgtttt ttcatccaac tgccaagcag aaccactgaa
     8221 tattgagcgc aatctattag gctcttttcc attgaccgga tcacgccaaa tataatagtc
     8281 ccgataagga ttttctttat ttttacgggc ctcaatgaac cacggatgtt catcagaagt
     8341 atgattcacc accagatcca tgatgatacg gatatttctt ttctttgctt cttcaatcag
     8401 ttcttccatt tcttccatcg taccgaattc tgccatgatt gcttgataat tactgatatc
     8461 atacccattg tcatcattcg gagactcgta tactggagaa agccaaattg catcaatacc
     8521 taaatacgcc agataatcca acctggaaat aatcccccgc aaatctccaa taccatcttg
     8581 atttgaatct tgaaaacttc ttggatagat ttgataaaca actgaatggt gccaccattt
     8641 ttgttccatg attttcctcc tataaatgga tgtttctatt ttttgcttaa tcatttattt
     8701 tagtctctct cactaaaaat tgtaacgctt tcaaatagct attgcacctt gttatcggta
     8761 acagaataaa acaaaaaaac agctgaggaa attccctcaa ctgttttttg aaaggattga
     8821 tttatgcagg gaagaatgct gatttcacaa cttctttata aacggtgttc actcagctgt
     8881 cccttggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg tttttttctc
     8941 taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca atatccacat
     9001 gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt ttcctctttt
     9061 agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat ctgaaatact
     9121 ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct ctaagtttcc
     9181 ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca atgctggtgc
     9241 agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta acaagaagaa
     9301 aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac cagctgatga
     9361 ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca tagctgttga
     9421 atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga aacctgcttg
     9481 gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca agaatgtcag
     9541 cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg caactaaaaa
     9601 tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa tcaaacctgt
     9661 gaccagacct aaaatcgttg tagctgccat cccgatgaag atgccacctg gtacacgccg
     9721 aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag aaccaacgtt
     9781 gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac cttcgctcag
     9841 accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca aatctgctgg
     9901 gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa aaatcaaaga
     9961 agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta aacaaacaga
    10021 ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat aacgggctaa
    10081 tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa acactgctcc
    10141 ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat aagccattga
    10201 gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca atttttctat
    10261 ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg aacattataa
    10321 ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca tatactaaaa
    10381 caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt
    10441 aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat ttctttattg
    10501 tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg attttaccat
    10561 ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga ttatttagtc
    10621 cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt cctgatttga
    10681 gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa taaccttcta
    10741 atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga ctcagctttt
    10801 cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat gccattgtta
    10861 tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc atccgtaaaa
    10921 aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac aaatactttt
    10981 gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt aaattcttct
    11041 ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa tcggcaaatc
    11101 atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat ttgacccttc
    11161 tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg aaagcgcaaa
    11221 caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga ttgctttcct
    11281 ccacaaactt tttttacaaa aaaaaaaacg gcaatgaatg ccgctcttct tatttcgatt
    11341 cttttataat tctgcttctg attcatcttt ggttagtgtg tgtctaacac tagttacgcc
    11401 aaatgtttga tcgatttttt ccttcaacgc ttcgattgcc tgtgcgccac gtgccgcttg
    11461 ttcagcattg atggattcaa tgaccagcac cgctgcttgt gtttcttccg tcagcatcgt
    11521 tctttgggct tcacgccagt tgagacagcg acagatcgct ccttgttcat catagtagat
    11581 gatttcttcc ggaagtgccg gagcatcgga atctgcacct aaaggcagga atgattcgcc
    11641 accttgggct tttcctaaat gcagatttcc tgcgatcttt tcgatatctt ctccgccgca
    11701 aggtacgcca aaagacaagg aaatactatt atagatatct acaagcggat tgattgggaa
    11761 aaattcccgt tcttgatttg cacgtttcag taaagcttca atagaagaac gtgcaccttt
    11821 tttagtcttg aatgtggtaa atgcctgacg ccactgagca atgacttcgt tttggctgaa
    11881 tgtttcttct gtaaggtaat ttcttgcttc ttttgctgcc tcattcaata attgatgata
    11941 cgtttcttca ctttcttttg ttacgtggtt atcgatacca tttacggata agatttcgat
    12001 ggttgcttca ggaaatactt cccagaaatt ggtatccact attacttttt tcatctgttc
    12061 acacctcttg attagaataa ttgcttgatg aatagatagt ctgtttgggg atcatctccc
    12121 atataaaaaa catgttcgct gtatttgata aatccttttt tacgataaaa agcgagcgca
    12181 acatcgttgt gttcccaaac accgagccaa atggctgttt tttgcttttc ttttgctttt
    12241 tcaatagctg tatctagtaa aaatgtaccg ataccttgat gtttaaattt ttttcgtaca
    12301 tagattcgct cgatttctaa aaaatgatca gccatttcct cagattgact cgttcctgta
    12361 tttagtttca ggtaacccgc aggctcttga tcgatactca ctaggaaaaa atcggtttct
    12421 ttatctttta gctcttctgt aagtttttct agtgcataag cttgtgtcaa gtaatcagct
    12481 aaatgctccg gtgaattatc ttttccaaat gtatctgtaa atgtttcgat actaatttgt
    12541 tgtagtaagt ctacatctga aggcgtcatt tttcgaattt cgtatttatt cacggcttct
    12601 tcctttcaat agatcctttt attgcctttt ttgacaaaat tccaatcttc gctaacattc
    12661 ttgcatactt tatttaaaag atattccaat tgttttgctt cttcgtcaga aagaccattt
    12721 aatgcgactg cattggagta gttgttttct cgaatgatga aaggatagat ttctgctcct
    12781 tttttagtcg gatagagttt cttgattttt ttattttctt tatcttccaa acgttcgatc
    12841 atcccattgg attctaattt cttgattgct cgggcagtcg ttgtacgatc tactttgatc
    12901 atttctgcta gtttttcttg gatgattcca ggattttcgc aaatacgtac taaataaaga
    12961 tactgtcctc ttgttaaatc gacttctttg aattcgatat tgctaataga atctagcgct
    13021 cgggcaatca ttccgatgtc tcgaaggatt tcagccattt catcccacct ttattttgtt
    13081 tgtaagacta gttcacctct ttttgttgca aatgcaacta ttttcaataa aaaaatcaag
    13141 ttctttttta ataactagta aagtatattc ttttcttctt ttacgaaaaa gctcaatcac
    13201 aatagatctg gtttttgttt tctaaaataa gtaactgttt cttcgttgtt cgcttttctg
    13261 atagctttgg caactcttct atactaaaaa atcccatgtt ggaagtttca ttattctcaa
    13321 taaacttggc ctcttcaccg tggatcgcac aagcaaaaac aagtttataa tactgaaaaa
    13381 gctgtggaat atctttttgt ttattcgtat caaaaactgc tcgcaactga gtagtttcta
    13441 ctgttaatcc tgtttcttca tagacttctt tacgcacatt ttcttcagga gacaatccta
    13501 tttcagcaaa tccaccagga agtgaccatt ctttcgtccg tttatcttcg attagtagga
    13561 ccttgccatc ttttttgata taggcacgaa catctacttt tggtgttgga tgtccttctc
    13621 caatcgttgt gagcgctttt atttcttcga aagtttcttt tcctatttca ctgatcagtt
    13681 ccaatgaaat cgtccggatc tcctcaaaac gctctttatc aaatacatct tttccataat
    13741 atagaccagc ttgagaaagt gcgagtagtc ttttgtattg agccaacttc ttgttcattc
    13801 tcttcccttc ttttttagca aatgtatgac tatgtaatca agtatagaag actggtttaa
    13861 ttacggcaaa taaatataat gaaatctgca ataaataaaa gaatgttaaa aaactcgaga
    13921 acaaaagaca ttcgacttct ctggggctgt gaataaa
//
LOCUS       DACENJ010000056        13906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.056, whole genome shotgun sequence.
ACCESSION   DACENJ010000056 DACENJ010000000
VERSION     DACENJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.056"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(2..1204)
                     /locus_tag="IW748_001220"
     CDS             complement(2..1204)
                     /locus_tag="IW748_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1104139.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1321..1836
                     /locus_tag="IW748_001221"
     CDS             1321..1836
                     /locus_tag="IW748_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1104140.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2035..2481)
                     /locus_tag="IW748_001222"
     CDS             complement(2035..2481)
                     /locus_tag="IW748_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1104141.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2528..4006)
                     /gene="msr"
                     /locus_tag="IW748_001223"
     CDS             complement(2528..4006)
                     /gene="msr"
                     /locus_tag="IW748_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1104142.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4360..4989)
                     /locus_tag="IW748_001224"
     CDS             complement(4360..4989)
                     /locus_tag="IW748_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1104143.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(4973..5437)
                     /locus_tag="IW748_001225"
     CDS             complement(4973..5437)
                     /locus_tag="IW748_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1104144.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5924..7192)
                     /locus_tag="IW748_001226"
     CDS             complement(5924..7192)
                     /locus_tag="IW748_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1104145.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGFTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7423..8160)
                     /locus_tag="IW748_001227"
     CDS             complement(7423..8160)
                     /locus_tag="IW748_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1104146.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8227..8796)
                     /locus_tag="IW748_001228"
     CDS             complement(8227..8796)
                     /locus_tag="IW748_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1104147.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8924..9796
                     /locus_tag="IW748_001229"
     CDS             8924..9796
                     /locus_tag="IW748_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1104148.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10138..11010
                     /locus_tag="IW748_001230"
     CDS             10138..11010
                     /locus_tag="IW748_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1104149.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11007..12965
                     /locus_tag="IW748_001231"
     CDS             11007..12965
                     /locus_tag="IW748_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1104150.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13216..>13906)
                     /locus_tag="IW748_001232"
     CDS             complement(13216..>13906)
                     /locus_tag="IW748_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HAQ1104151.1"
                     /translation="DDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSY
                     IAYTSFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQ
                     FVYAAGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKE
                     LEIIEASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSL
                     EAALKEILDQE"
BASE COUNT         4162 a         2863 c         2530 g         4351 t
ORIGIN      
        1 cttattcttc ttcatttgta gcaaaataat tacggtgata aatgatacaa catacactga
       61 taagaacaat ggcacctgca aaataaaacg catagctttc accaatatgt tcaccaatca
      121 cacctgcaat aaatggtgct aaagctccac cgaaaaagcg gacaaaacta tagctggacg
      181 aagcaacgtt tcgttgcaga cctggtattt ccatagcaat cgtcgtcaac agtgtattga
      241 taattccttg gaaaaatcct gctaacacaa tgctgatcgg tacaaggact ggcatagaag
      301 ttccagctcc taataacagc aaacaaataa tgaataagac aaatgctgct aaaattgtcc
      361 gataagtcgt aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca atagccagaa
      421 gcacacccca gccaaaaaat acaaaaccga cttctaattc agaatagcca atcaataaaa
      481 atggcgcata cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg attagtccaa
      541 ctgaacgaag tttatgattg cttaacgctt tgacaccttc ccaaaggcct acttttctct
      601 ttggtttctc agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa
      661 atgctacccc aaaaaatgga taacgccatg aaccactgcc caagacccca ccgactagag
      721 ggccaacagc cattccgcag ccaagtgctg cttcatacat catgattgct ttttctgtgt
      781 taccaactaa aatactgacg atcgttgcta aagcagtaga gacaaataaa gcattcccga
      841 caccccagcc ggcacgtaac ccaatcagca ttccgatatt cccagataat ccgccaagac
      901 cagcgaatac aacgatgata aacaatcctg tcatcaatgt ttttttcaaa ccgatccgac
      961 tagataagaa acctgtaaat agcatcacca caccagtgac taacatgtaa ctagtaaata
     1021 acagggtcgt ttcagctggc gtagcgttta actgtgcagc aatcgttttt aagattgggt
     1081 ctactagacc cactcccata aaagcgatca tacaggtagc tgctacaact agcgctgaac
     1141 ttttcccctt tgtttcttca tttgtttgtt cttgtatatc tatttgattc tccaatattt
     1201 ccatcccttt ctctctatta gcatcataca tgatttattt tacatgtaaa tatgcacatg
     1261 taaaacgtgg aaactttact gtgatataat aaagaaaata agacgagagg taggattttt
     1321 atgaatactt tagcctatat attattatgc gtgcttttat ccaaaccacg ttctgggtat
     1381 gaattgaagc agctgatcac aattttttgg gaagcccatc acagtcagat ctatacaacg
     1441 cttgccaagt tagaccagca gggttatgtg accgttttgg acaaagacga gcactctcaa
     1501 aaaaagatct accacttgac tgatgaagga cgtgctcttg tagaggaatg gatcaaagag
     1561 gaaactcccg ctcctacgca aaaagatgaa tttctagcaa aaatctatgc tttttcgacg
     1621 ctcgataaaa caacagctac tggactgcta ttcactagag aacaacagct gaacaaaatc
     1681 atgcaaaaaa atcaaaaaaa attgaatgac ctctcgctag acaagaaaca agaatttgga
     1741 cgttatgttg tgatccagcg cagaattatg ctttgtcagc aggaactcca atggtgtcag
     1801 caagtaagag aacaaatgca gcagtttttt gagtaaggga taaacagaac ctttctagaa
     1861 atatctattg aagagcaaat tatcttcaat aaaagagcaa tacagttgga aaagaaaaag
     1921 cgatccgaac gacaggccaa aacctatcgt tcggatcact atttttctag aactgcattc
     1981 aagagactat gacaaaaagc atgccaaggc cttttttcaa ataacagtgt tccatcattc
     2041 attactttga attgctttta gcacacttct tgaaagcggg ccaacgatga tcaactgcaa
     2101 cggtaaagcg gcaatcacat tcattttcca agcattcata taagcagaga ataattgatc
     2161 aggtattcct tgttccatga tcacaccgaa taaagacata catgtcacca ttcctaaaac
     2221 catcaagcat gaaatagtta agatcatttg gaccttgctt tctggattta ctggtagttt
     2281 aaaggcaatt tttttcgcca gcacaccgat gatgaagaca tccaaaatga aagccaccac
     2341 gaatccaggc actagaccaa tcaacaaact tgataaatga agatcttgat gcagccataa
     2401 attatacgcg ctcatcccca agaccatcaa aaaacacatc attgttgtaa aaattattcc
     2461 ttctttttta tttgtaggca atctgtacca ctcctttaaa tttgtcacga aaatcatcgt
     2521 agcacattta aaaatttctc gtaagtactt tttttatttt ctgccctttt atttcataaa
     2581 cttcatcagc cgtacgctcg acaaagtaag aatcatgaga agtaaacaaa atcgttcccg
     2641 gatatatttg cattagcttc tctaaagctt cgatcgttgc catatcaata aaattagtcg
     2701 gttcatctaa cagcaacaca ttacttggct tagtaaaaag caaagcaatc gtcagacggg
     2761 tcgcttctcc cccacttaat gtacataacg gtttcgtcaa agtttcctga gcaaacccta
     2821 agtgattcag caaactacgg acaagcgatt ctgaaaactc tgtttgtctc atgacaaaac
     2881 gaatgatgga ttcttcagat gtcatttgat aacccttttg ctgatatact tgaaagctta
     2941 ctttcggaga gagaaggatt ccttctcctt gtttgcggat atgttctaat aaggttgtct
     3001 tacctgaacc attttcaccg acaatcgcga tttttttacc taaagaaaac tgaaaatcac
     3061 catttaccag taatgttctc ccacttcttt ccaattggac attttgtccc atgatgggat
     3121 agcggctgtg gagttcaaga gattttggta ctggaaaacg gatttgtttc atctgctgcg
     3181 gcttggttgt ttctcctatt ttttggagtc ttctctctaa tgttttcgca ttcttttgga
     3241 tggctttttg tacactgtct ttttgtttag aggaagacaa ccgatccggt ctgatttgtt
     3301 gctttttctt tttactggac actttttcca acttttccgc ttgttttttc ttttcttgga
     3361 tcgatttttc caatcgtttt ttctctttca tgatcgactc tgcttctcgt agctgagtct
     3421 ttttctccaa ttccttttga gtgagatagg cactataatt ccccgaaaac tctcggattt
     3481 ctccgtcttt tacttcccag attttcgatg ctagctcatc tagaaaatag cgatcatgac
     3541 taacaaaaac cagcgtacca taataataac gaagctcttc aatcagtcga tcaatacttt
     3601 ttctatctag atgggttgtt ggctcgtcta gaagcatccc ttgttcataa gaagaaagaa
     3661 gacgtatcaa ctcgatcttt cgttcttccc ctccgctcca tttccgctct gaagtttctt
     3721 ccagtcttaa ctggaaatga ctggctaatt ccaaatttag gtcctttgct tcagcgagat
     3781 aggttaattg aggcaggtag ttgaaggtga tctctgtttg gattttccca tgatcgggaa
     3841 aaagttcacc ggctattagt ttgagcaaag tcgatttacc gacaccattt tctcctatga
     3901 tccctatacg atcttgctga tagacacgaa gagaatcaat agatagttct agctggttgc
     3961 caaagctgac ttgtagattt gttatattta ctgctaaatt ttccatatgt tttacactcc
     4021 tatttcatgt aaaacaacaa ttatcgatac taaaaaagag cagaaaaatc tgtttgcaaa
     4081 aaaactgcat cacatctttt cctactctga acgaaataac tatgactaaa tcaccaaatc
     4141 tgtcatagct ttcacctatt ctactgtcgt tcgaaaaaat ggacatactc catccatgaa
     4201 attgttgttt atacatgtct tttgaattta tttagttgtt attcaaaagt tcgaaacgta
     4261 atttcatcga tgcagtcatt tttccgacct ccttttttat taggttacca cacacgattc
     4321 aaaaaaagca atagctactg aatagtaact gaaaaccttt tattcgaacg tagtgatctt
     4381 ttctggtgct aaacgaacag actcgtaacc ttcttctacc gcttttccta ttgataaaat
     4441 aataactgga acataacggt ctttatccaa accaaatgcc tctgccaact gatctgcttc
     4501 gaatccacca attggatttg tttcatagcc atgatcacgc gcaactaaca tgaattgcat
     4561 agccgctaag ctagaatcga ctttgactac atcgttcatt tcttctcttg aaaaactctt
     4621 gtaataagga atgatcgcag ctaattgttg atctctgact tcttttggca tcttccctga
     4681 ttcgtaagct tggttataga tttcttcgcc atattcgtaa cattgcatat cgccaaaaat
     4741 catgaccatt gcagaagaag tatcattttg tctcgtattg aaacggatca aaggacgcaa
     4801 gatttctttt gcttcctcgc tttcagcgac cacaaagcgc catggttgca tattgacaga
     4861 agaaggtgca gtcgtcgctt ctttgatcat ttccagcatt tcttcgtgtg ggattttgta
     4921 tgtttcatca tatacgcgaa cggatcttct accagtcata attgttgaaa aatcattttt
     4981 ttgttccatt attattaacc tctttcgttt ctgttgttat attgcaaagt tgggtattca
     5041 atttatctaa aagtgttaga aattgtttca tttctacatc gctgaaagcc ggggatagca
     5101 cggaacctgt atctttatgg tccatttcac aatgctcgat tttcttctgg gctttttctg
     5161 ttagcgctac atacacttcc cggttgttct gaggatttct ctgacgcgaa acatagccat
     5221 ttgcttccaa taccttcaga tgacgagtaa ctgctccttg atcaatctgc aacttttctt
     5281 gcaaactgct ttgtagcaat ggattttctt ctttcagtct catcatgatc tcgtatctag
     5341 taagacttat accgatattt tcttcaaaga tctggacgat tttttgatcg gctaatttga
     5401 tttgatataa aaggtgacta atctcttcta aagacataat ttcctccttt ctaataattg
     5461 actcgtcaac gattgacttg tcaataatag aataaaaaaa gaaaaaagtc aaagaatcag
     5521 ctgtgtaata aaaaaaacgc tctcaataga aaagtataga gaaaagaaat tttttgtaga
     5581 atgaacggaa aaaatgataa agaaagcaca acgctaaaac caagattatt gattcttatc
     5641 aaactataga agatagctct tctgcagtag aaaattttac accgccaaaa atttccgaaa
     5701 tcgccttttg caagaatccc gtcgaatatt agaaattgga tcagtatgct taaacagtaa
     5761 taaaaaagtt atgacaagat gttgtcacaa ccttttttga gtaaacggtg ttcaaaaact
     5821 tgcatctgcg gtaataagtt caaccaaaca aaaaacatga aaatctgttt ttgtttggct
     5881 gttcttacta cttgatgcag ggcagctttt tcactacctc tttttaataa ccataaaatc
     5941 ctattccttc atatctccag cccatcggtg ttagccaatc tctttcctta gcactcaagg
     6001 tatagtgatg ggtaccagtt ttttcatttg gattaaatag tcgataaatt ggcttggaac
     6061 caccagaata aaaacttaga ccttcataac gccaaccgat ttttgttaaa gagttgatct
     6121 cagattgtgc cattgtgtaa tgatggtctc cactgtaagg attgtataaa cggtagacag
     6181 gttgtccgct gtttggtgct tgccaagcaa tcccttcata ttcccagcct acagttggca
     6241 gataattttt ttctccggca ttcaatgtat ataaatgttc tcctgtatga cggttataca
     6301 gacgatacat cggagaagtt gaagttggtg ctgtatcata ctgtgtcaga ttataggttt
     6361 cgattacatt gttcaatgta aaaccataat taggcgcagt ggcataacga cctgttagcc
     6421 aagccgtagc atctttgtaa gagttcgtat tgcttttcca tgcaccggaa tagtagtaaa
     6481 cgcctggctg aaaagaagtc gtcttcaata ccgttacgtt atcttgcaat gattctttgt
     6541 acgaaggata ttttctaaaa gcggcattca ccgtaatcca ttgcccattc acatattccg
     6601 aagtaggcat cgtaacagat tggccattat agcttccttt aatcccaaat agattgtaat
     6661 taggggcaga agccaaagta ctgttccccc agccactttc aacgatggct tgagcaatca
     6721 tgactgatgc atataagtca ttggcacttg ccaaactttg tgcagttgag gcaatactat
     6781 caatgaacgc ttgttgagaa ttagcagatg agcgagcata aaaagcatag ccagtatagt
     6841 tttcgaattc agacaaatct gcttgatggg tatgtgtcaa tgtttgccca tctgtcaatt
     6901 ggttcgtgat ttcttctgca ttctctggat tgttctgagc ttctgctgat gtactgtctt
     6961 cggtttgtgt ttgttctgtt gtttcagttg ttggttcttc ggttgaaatt gtattttctg
     7021 agacagtagc tgactgcaaa gtactctctg tatcgttttc aattcctgtt tctgaagaac
     7081 tcgatgtctg agttgcgaca gtttctgtcg attcttcagc tagcactgtt gtgttggcaa
     7141 ctgctagatt tcctaaaaga ataacgctaa ataataaatt ctttgctttc atcttttttc
     7201 tcctttgaat tatgaaactt ttcctaaata acagtgtaac taaaaaagta aaagcaagtc
     7261 aatgtccata tttaaataac atttgtttct ttttgataaa tttgcttttt ctacataaaa
     7321 agacgtaaat gaagaatatt cttctccatt cacgccttaa attgatggtc gtttgaataa
     7381 ctatttaaca agtaattctc tttggatatt atccgttatg gattatactt cctgtgtcgt
     7441 cggacggata atcatgttac tgacagacat ccggtctggc gtttctatcg caaatgctac
     7501 agcagaagct atatcttcgg aggtcaatcc ccattctttt tgcgcttcat gaagttcaag
     7561 agcagccttt ttgtcgctga tggtttgata taattcagtt tgaacagctc ctggagagat
     7621 gatcgtagat ttgatgttat tttgccgttg ctcttgacgt aatccttcca tgattgcacg
     7681 gacagcaaat tttgttccgc aataaacggc ggaatcagga taaacaacat gtcccgccac
     7741 agaatcagtt gaaatgatat gcccagattt ttgtttttcc atgattggaa gtacggcgga
     7801 aattccattt aacacaccca tgatgttgat atcaagcata ttttgccatt catcacggtg
     7861 accttcaact aatggagcgg ttggcatgat tcctgcatta ttgtacaata catcgattcg
     7921 accgaacttt tccatcgcta aatcgataac tgcttggact tgtgcaaaat ctcttacatc
     7981 tgctgtttga atatatagct cagcttctgg taaagattct tttattgctt ttaggcgatc
     8041 ttctcggcgt gcagctataa ctagtttagc ccctttttct gctaatagct tcgttgttgc
     8101 ttcaccgatt ccgcttgaag cgcccatgat gacaatcact ttttctgata aatcattcat
     8161 tctattctcc tcctgttttg aaaaatctct tgcttatact cactatctct tatttcatct
     8221 tactgttcaa ataatccgtt cgacaggttt gacgattttg gcagggattc ctgcaacgat
     8281 ggtattgtct gggacatctt tcgtaacagt tgcattcgct gcaacaatgg cattctcgcc
     8341 aatcgttacg cctggcagaa tagttgctcc tgctccgatc caagcatttt tcttgatgtg
     8401 gattggcttt actgacaagc ctcttcgttc ttttggatcg gttagatggt tcaccgtgat
     8461 gaggttgaca cgaggaccaa tcaatactga atcttctaaa cagatgccac ctaagtcaac
     8521 gaacatcact tgacgattga taaagacatt tttccctata ctaatatgtt tgccgaaatc
     8581 tgtataaaaa ggcagcagaa cagtcaccgt atcatctact ttgtcactaa tgatattttc
     8641 tagccgtttg cggatatctg ctggttcatg gtacccagta ttcagcgctg ctaaaggctc
     8701 ttcgttttca gcttggatct gatggatcac ttcaaagagc ggatccgtta ctcgaatctc
     8761 tttattgacg atttgttttt cgatgaggct tgtcattttg ctccctcctt ttatccttac
     8821 tttataacgg acggcttatc atgtctaata cttataaaac atacttaact attcctttta
     8881 actatactta gtggtatgct tttttaaaaa caggagggaa aaaatggata ttcgtatact
     8941 gacgtatttt ttagcagtag ctagagaaaa aaatatcagc cgagcagcaa aatctctgca
     9001 tatcacgcaa cctactctct caaaacaact gaaagattta gaagaagaac ttggtgtgac
     9061 acttttcact cggggctccc gagagataca gttgacggat tctggtcgat atctttattc
     9121 aaaaggaaaa gaaatcttgt cgttggtaga aaaaacggcg aacaatttaa caaatgacgc
     9181 aatcatctct ggagaaatct acattggcgg aggcgaaact aaagcaatct ctttcatttc
     9241 tgatagcctt cattcgttga tcaaggcgca tcctgatatc cagtttcatt tatatagcgg
     9301 gaatgcagat gatatcatag acaaactgga tagcggatta cttgattttg gactcgtaat
     9361 cgaaccgaca aacaaacaaa aataccagtc atttcgactg ccagcccaag atcgttgggg
     9421 acttttgata catcgaaatc atcctttagc aaagcaaacc gtcatccatc cgcaggaact
     9481 gaaaactgtt cctttgatca tttcccggca aacgtcagtg gacagtcaac tagctgaatg
     9541 gctaggtttc agcatagatc agttatcgat cgtcggaaca tataacttat tgtacaatgc
     9601 ttctttgatg gtcgataacg ggacttttgg tgcattagct ctagatggga tcatcaatac
     9661 ttatgggacg gaaaaaatat tcatcccact cgatccgcca ttacaagctt cactgaatct
     9721 catttggaaa aaagaccagc ctctttctcc tgctgctcgt aaatttttag atgtcctaac
     9781 ggagaaatac agttaagtag agtttttttc cttcgctttc agctttttaa caacgaataa
     9841 gataagcaaa agatccaacg attaaataac ataagtttct taatttattt cttcctttta
     9901 tttctgataa ttccagtaaa ttactataat tataaaaaaa gaattgacaa aacataactg
     9961 tctatataaa attgattacg ttaacaataa ttcgtttcat gtgactagat catactaggc
    10021 atgaaaaatc tctgaggaac atggtttccc taggaggttt ttcgtgtcta tttttgtttg
    10081 gccgcaatca actggtctag caaacgtgaa aaccaaatct tcaaaaaggg tgcgcgcatg
    10141 aaaatcaaaa agatctttaa tcaaaatgca gtattggtag atgacggtgg acaggaaaaa
    10201 gtagctattg gaaaagggat tggatttgac agaaaaagaa atgatttgat ttttgatcgt
    10261 gatgtcgaac ggatatttgt catggaacca gaaggtcaaa tcaagttgca aaatttactt
    10321 aatcagattg atgaacgctt tttatttgca gcagaaacga tcattgatca tgcggaaact
    10381 gttttgatgg aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca cattgctttt
    10441 tctgcggaga acattaataa cggtatcgtc atccgcaaca aacttttaag agaaatcgaa
    10501 gtgctttaca gtgatgaatt cagtattgct caatgggcag tagagtatct gacaaaagag
    10561 cttgggattc catatactta tgacgaagca ggatatatcg caatccatat tcacagcgga
    10621 cgaagcggtc ggaacaataa ccatcgcagt atccgtgaag tgacgattgt atctgacatc
    10681 atccatttag tggaatcaga attggaaaca gatatccatt cagaaccatt ttcactgaat
    10741 tattcaagac tggtgaatca tttacgacta cttctacaac gaacccatgc gcaacaatat
    10801 gctgttttag acacggaaat cgtagaaatg gtcaaacgaa aatacccaga aagctataag
    10861 atttctaaaa aaatccgtgt gttgctgacc aaagaatacc agctcgcgat cacgaaagaa
    10921 gaactgggtt acttggccat ccatatcgag cgtttaagaa gcgcaatcgt acaaacaaat
    10981 gttaacaatc atgaggagga aaaaaattga aagcatatct acaacgaatg ggacgttccc
    11041 ttcaacttcc agttgcggtc ttacctgctg ctgctttact agtagggatc ggaaactggt
    11101 gggcatctta tagtaacgac attgtcgctc attttttaca agctggcggt aacgcagttt
    11161 taggtcaatt acctttgctg tttgccgttg ggttagcact agggatgtca gtggacaaat
    11221 caggtgctgc tgccttggca ggtttggtcg catttgaagt tccagtaaat gttttaaaaa
    11281 ctgattcagt agctacttta ttgaatatca aaccagaatc agttgatcca tcttttgctc
    11341 agatcagtaa cgtatttatt ggtattattg ccggattgat tgcggcagct ctttataatc
    11401 gtttccacga agcaaaatta ccgatggctt tatctttctt tagcggcaag cgtctagtgc
    11461 caatcttggc tgcctttgtc atgttgctga tcagtgcagt gctactgctc gtatggcctc
    11521 cggtttactc tgctttagtt tcttttgcta aattcatttt gagtttaggc tggggtggtg
    11581 caggtcttta tggcttcttc aaccgtttac tgatccctac aggattgcat caagcaatga
    11641 actccgtgtt ctggtttgac gtagcaggaa tcaatgatat tggtaaattt ttggcttctg
    11701 aaggaacaaa aggcgtaaca ggaatgtatc aagctggatt tttcccaatc atgatgttcg
    11761 ggcttccagc aggagcatat gctatttaca gaaatgcacg tccagaaaac aaagcaaaaa
    11821 cagcttcctt gatgctagca gcagcttttg cttcattctt tactggtgtg acagaaccac
    11881 ttgaattttc atttatgttc gttgcttggc cattatatgt cttgcatgca attttcactg
    11941 ggatttcttt agcagtgagt gcattcttcc attggacagc aggatttgcc tttagtgccg
    12001 gatttgtcga ttacttccta tctttgaaaa atccgattgc gaatcagcca ttgatgttga
    12061 tcgtccaagg attagtaaca gcagtgattt actactttgg ctttaacttt gctatcaaaa
    12121 aattccatct gatgactcct ggccgcgagg aagcagatct ttcagatgac gatactgcaa
    12181 cgactaattc agacaataaa tatgcggcac aagcaagcaa aatctacgct gcattaggcg
    12241 gagccgacaa tgtaacttcg atcgataact gtactacacg ccttcgtttg caagtcaaag
    12301 atacaggcct gatcgatcaa aataaaatca aagccactgg cgtacctggt atgaagatca
    12361 tcgacggaaa aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt gcagatgaga
    12421 tggcaaaact acatggtggt gctgcagcac gacctgcaca aacaaacacc gtggttaaaa
    12481 cagaaacatt tgccggtgaa acgatcgaac aagatattta cgctatcgca aatgggaaac
    12541 tgattccaat cactgaagtt tcagatgatg tattcgcgga aaaaatgatg ggtgacggct
    12601 atgctgtatt accagaaaat ggagaaatct tctctccaat cgctggtacg atcacaaaca
    12661 ttttcccaac gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa gtcttgcttc
    12721 atatgggcat caacactgtt gacttaaaag gcgaaccatt cactttatat gtagaagaag
    12781 gtcaaaaagt cgcaagaggt caattgattg ctttagttga tcttgccgct attcaatctg
    12841 caggcaaaaa cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc caaagcttag
    12901 aaatcgaacc cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt tccaacaaag
    12961 cctgagccat cgtcttcccc agactttttt atcgataaac aaacaggagc tgagacaaaa
    13021 atcttgtctc agctcctttt tccgaataaa aacggcgttc tcctgccaac tccgcggtaa
    13081 taagtccagc taaacgaaac atatgaagag ctatttttta tttgactctt cttattactt
    13141 gatgcagaac agctttttca caaaattttt cttcatactc aaaactaatc ggctttttca
    13201 caacctcttt atacattatt cctgatccaa tatttctttt aatgctgctt caagagaagt
    13261 taatggtttt cccaatactt tttctaaatc atcagaggaa atagctaact gatccttttt
    13321 aatatcatgt tggatcgcta aaaacatgtc agctgcttct tgagggaatc cggcttcttt
    13381 tagattttcg acaaacgctg catcacttgc ttcaataatt tctaactctt tacctgtcgc
    13441 acgctctaat gccttggcta attcttcata gtcagttaga ttgccggata attctaagat
    13501 ttctggagag tcactttcta ctagtacttt tgctgcagct tctgcatatt cacgtttcaa
    13561 tgcccaacct gtttttccat ttcctgcagc ataaacgaat tgaccagttt ttaaagcctg
    13621 cccaatgata ggtaattcat tctctacata ccaattattc cgtaaaaatg tatgggaaat
    13681 acctgaatcc ttaatcattt tttcagtgaa ttggtgatct ggcgctaata cactgatcga
    13741 gtgatcggca tctgcaaaac ttgtataagc gatataagat accccagctg cttgtgctgc
    13801 cttcactaca ttcttatgtt cggcttgacg atttcctgga acgccagaga caaaaagcaa
    13861 tcgatctatt cctttcaacg cttctttcat tgagtctaaa tcatca
//
LOCUS       DACENJ010000057        13816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.057, whole genome shotgun sequence.
ACCESSION   DACENJ010000057 DACENJ010000000
VERSION     DACENJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.057"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(159..1469)
                     /locus_tag="IW748_001233"
     CDS             complement(159..1469)
                     /locus_tag="IW748_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1104152.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IW748_001234"
     CDS             complement(1481..2899)
                     /locus_tag="IW748_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1104153.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IW748_001235"
     CDS             complement(3139..4707)
                     /locus_tag="IW748_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1104154.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IW748_001236"
     CDS             complement(4711..5121)
                     /locus_tag="IW748_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1104155.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IW748_001237"
     CDS             complement(5118..6041)
                     /locus_tag="IW748_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1104156.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IW748_001238"
     CDS             complement(6038..6916)
                     /locus_tag="IW748_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1104157.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IW748_001239"
     CDS             complement(6903..8240)
                     /locus_tag="IW748_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1104158.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IW748_001240"
     CDS             complement(8243..9346)
                     /locus_tag="IW748_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1104159.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IW748_001241"
     CDS             complement(9420..10928)
                     /locus_tag="IW748_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1104160.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IW748_001242"
     CDS             complement(10894..11586)
                     /locus_tag="IW748_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1104161.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IW748_001243"
     CDS             complement(11608..12582)
                     /locus_tag="IW748_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1104162.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IW748_001244"
     CDS             12882..13754
                     /locus_tag="IW748_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1104163.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
BASE COUNT         4088 a         2931 c         2278 g         4519 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaagg
//
LOCUS       DACENJ010000058        13644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.058, whole genome shotgun sequence.
ACCESSION   DACENJ010000058 DACENJ010000000
VERSION     DACENJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.058"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..1035)
                     /locus_tag="IW748_001245"
     CDS             complement(94..1035)
                     /locus_tag="IW748_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1104164.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1161..2282
                     /locus_tag="IW748_001246"
     CDS             1161..2282
                     /locus_tag="IW748_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1104165.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2540..4042
                     /locus_tag="IW748_001247"
     CDS             2540..4042
                     /locus_tag="IW748_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1104166.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4109..4636
                     /locus_tag="IW748_001248"
     CDS             4109..4636
                     /locus_tag="IW748_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1104167.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            4847..5908
                     /locus_tag="IW748_001249"
     CDS             4847..5908
                     /locus_tag="IW748_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1104168.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            5911..6807
                     /gene="murQ"
                     /locus_tag="IW748_001250"
     CDS             5911..6807
                     /gene="murQ"
                     /locus_tag="IW748_001250"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1104169.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            6821..8803
                     /locus_tag="IW748_001251"
     CDS             6821..8803
                     /locus_tag="IW748_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1104170.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(8868..9314)
                     /gene="nrdI"
                     /locus_tag="IW748_001252"
     CDS             complement(8868..9314)
                     /gene="nrdI"
                     /locus_tag="IW748_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1104171.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            9525..10949
                     /locus_tag="IW748_001253"
     CDS             9525..10949
                     /locus_tag="IW748_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1104172.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11130..11903)
                     /locus_tag="IW748_001254"
     CDS             complement(11130..11903)
                     /locus_tag="IW748_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1104173.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(11900..12805)
                     /locus_tag="IW748_001255"
     CDS             complement(11900..12805)
                     /locus_tag="IW748_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1104174.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(12959..13279)
                     /locus_tag="IW748_001256"
     CDS             complement(12959..13279)
                     /locus_tag="IW748_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1104175.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4343 a         2442 c         2898 g         3961 t
ORIGIN      
        1 catcatgaga gttgtgtttt ctttttgatc ttttattggg cttaaaaaaa gagaaccagc
       61 tgctgattca gctgattctc atcagagagc cttttattct gcgccaagga ttccttcttt
      121 tgcatggact aattgggtca aaaaggcaca atatggtgta gcaatatcat atttttgtcc
      181 ttttcgccaa atggcaccat tgatgtagtc gatttcagtc aaacgatggt ttttgatcag
      241 gtcttggtac atagatgggt aatgcaaccc gatgttgtca ggatcataag tagaagcaat
      301 atgttggtac acttcttctt gatctagtat gattccttct tttgctgcga tagaagcaaa
      361 ttcagatacg attgttcgaa ccatgctctc tgccgctttt tgcgcgccta gttcagcaat
      421 attacaatcc aaaatcgtgc ataaaccatt caatgttcca tttacacaag cttttcgcca
      481 aatggaataa cgaacgttat ggctatagat cggatttaaa tttgcttctt gaaagacttc
      541 tacaactttt tttgtaaatg cttcgccttc tggttccaag ttctctagtt cgatttctcc
      601 atcacctaac aatgtgactt ttccgggacc agctagacca gctgtccaca tcgtgatccc
      661 aaataagata tttttctttg gcacatattt ttccagtaca tcttcatgac ctaatccatt
      721 tagtaaacac aaaacataag tattgtcagt aataatggat tggatcgaac aaaacatatc
      781 atctaattga ttggcttttg ttaatgctac gatcaagtcc acgtgttcgt tgctttcgat
      841 gatttcttct ggtgaataga ttggcagttt tgcgaccact tctttaccgt taaagtcagc
      901 aatcaaccca tttgtacgaa tggcttcaat gtgagcaggc caacgatcga tcaagaggac
      961 ctcatttccg ctttgatgta gcatcaatcc gattcggctg cccattgctc cggccccagc
     1021 tattgcgatt ttcatgtttt ttctctcctt tattcaatga gttccctttt agtatataga
     1081 aaaatggaag aaaatagcat tgttttgttt gaaaaaaatg aaaaaaagtg tcttacttaa
     1141 gaagaaagtg agggatacag atggatattt tggcggcaat tgattcattt aaaggaagtg
     1201 cttcttcatt tgaactaaat caggcagttc taaatggctg ggaagatcgt aaaggaagaa
     1261 aaatcaatgt gccgattgca gatggcggag aaaatactgc agaagcaatc tattacgcgc
     1321 tcaggggaac atggcgtacc attgatggga tggatctctt attccgtcag cgaaaaatca
     1381 gctatttatt gacaacttgg caaggaaaaa acaccgcagt catcgaatct gcacaagcat
     1441 tggggttaga tctacttgat aaaccaacgg atcaaactat ccgacttgct tccagttatg
     1501 ccttaggaga acttattaaa gatgcactgc ttcaagaagt agaacaaatt attgtcactt
     1561 taggaggaag cgggtgtagc gatggtggtc taggattact ccaatccttg ggtgcttctt
     1621 tagaagggta cacagaagga aatcctttat tatctactaa aaaacttcat tttgatcgaa
     1681 gaaacgttct tgtttctaaa gtaaaattga ttatcgctgc agatgttacc aatccatata
     1741 caggtcaaca aggagctgct tggcaatttg gaagacaaaa aggaggaata gatacgacgc
     1801 tagctttctt agataaacaa gcaggaaaga ttgctcagca aataaaagat ttctgggagg
     1861 tggatttaga tgatattcca ggaagtggag cagcaggcgg tattggtgga gccctttttt
     1921 tgttgggagc gaagatggtt tctggtttcg atttagtcag ccaaatcact ggattggaaa
     1981 aaaagatcga acaagcagat gtcatcatta caggagaagg gcggatcgat cagcaaacga
     2041 tccacggaaa agttccatac ggtgtagcag tgcttgcaaa aaaatatcga aaaccaataa
     2101 tcgctatttg cggaagtaga gaccaagagc tagaaggact ttctgatttt ctcccaattg
     2161 tcttaagtat tcatttgggt ccaataggtt tagaagaagc aatggaacac aggcaaacat
     2221 tagaaaaaac aaaaatctta ggacagacac ttagcagatt tttcaccatc ctcccgaagt
     2281 agttgacaaa atcattttct ttatgctatc gtaatttcaa atataggaca aagtgaggga
     2341 tccgtgcatt gaaaaattaa gtcgattgat gaaagctgca ttcagactgt accttgtgga
     2401 gacaaggaag cagtgtgcag attttcggga ttggcttggt tttttcgatg tatgggtctt
     2461 tttgtgtgta tccaaccctt gcatcctctt tagccagttc tgaaaatagg actggtcttt
     2521 ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa aatctgacat tcggctacga
     2581 cagccaaggc acattattat ttgaacaagc aaatctaaat tttgacacac aatggaaact
     2641 aggacttatc ggacgaaacg gtcgaggaaa gacaacttta ctgaatattc tacaaaacaa
     2701 actaccttat caagggcaag taatccatca gcaagaattt gcctatttcc cgcaacagac
     2761 aaaagataaa gaacgtttaa cctattacgt gttaaatgat attacggatt ttgagatatg
     2821 ggaaatcgaa agagagctcc aattgatgca aacagatcct gaaatcttat ggagagaatt
     2881 cagcacacta tcggggggag agaagacaaa agtcctactg gcacttttat ttgtggatga
     2941 cactcatttc ccgttaatcg atgaaccaac gaatcatttg gatatctctg gtagaaaaca
     3001 agtagcggct tatttgaaaa agaaaaaaca aggcttcatc gtggtcagcc atgaccgggg
     3061 atttatcgat gaagtagtgg accatgtttt agcaatcgaa aaaagtcaac tggaacttta
     3121 tcaagggaat ttctctatct atgaagaaca gaaaaaactt cgtgatgaat ttgaaatggc
     3181 tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag aaaacagcag ctgaaaaagc
     3241 cgaatggtct cgttcccgag aaggagataa aacaaagaaa caagtcggat tcatcgatac
     3301 tgaatctaga cgagtgaata aaggagcagt gggtgctgat gctgcacgga cgatgaaacg
     3361 atccaaagca atcgtgaatc ggatggagac ccagatcagc gagaaagaaa aactattaaa
     3421 agatatcgaa tatatcgatt ctttgacgat gaatagccaa gcgtctcacc ataagcgact
     3481 tttaagcgta gaagatcttc aattagggta tgaaaatctg ttattcgagc caattcattt
     3541 tacaatcgag cctcatcagc gggtggcgat ttcaggtcct aacggtgcag gaaagtcatc
     3601 cattatccat tatcttctgg gggcattcaa cggcaaggtt ataggagaaa aataccagcc
     3661 aaaacatctg agcattagtt atgcaagcca aaattatgaa gacaatcgag gaacgttggc
     3721 ggaatttgca gagaaaaacc aagtagacta ccaagcattt ttgaacaacc tccgaaagct
     3781 tgggatggaa agagatgttt ttcataacaa gatcgagcag atgagtatgg gccaacggaa
     3841 aaaagtggaa ttggctaaat ctttatcaca gccagctgaa ctatatacat gggatgaacc
     3901 attgaattat ttggatgtct tcaatcaaga acaattagaa caactgatct tgaacgtgaa
     3961 acctgccatg ttactagtgg aacatgatca aaccttcctg gataaagtat ctactgagat
     4021 tatttctctt gagagaatct aacccttgta atctcaagaa caacaaggta tggttaaggg
     4081 gaactttgcc aacgaaggag agaatcacat gctttatgta acgagacacg gagaaacaac
     4141 ttggaacgca caaggattag tttgcggacg ggcagatgtg ccgctgactg aaaaaggaca
     4201 gatgcaggca cagaaactag cagaaaaggt agtagatttg cctgttccaa tcacgaaaat
     4261 catccattcc cctcttcaac gagcaagaga tacagcacaa gcagttgctg atcgtctcag
     4321 cttgccgctg accgttgatg aacgattggt agaaatggat tttggggatt atgatggact
     4381 gccaagcaaa gatgagaact ttcaaaaagc acgtttagcg ttcgctgtac gattcccaaa
     4441 tggggagtca gtcttagatg tgtatgcacg gatcgttcct ttattgaaag aatgcatcga
     4501 agacgaagaa aatgtctatc tgttagtttg ccataatgcc ttgataagag tgatcaatgc
     4561 ttattttcat ccgatgccta atgaggggtt ctttacattc atggtagata atacagaatt
     4621 ggtttcttat gagtaattat gtttttgtgt tatttaaata aaatatttca aggcactgct
     4681 tttgcttagc agtgcctttt ttaggttatg acaattcttt tgataatata aactcaacct
     4741 ttagaaatga aatttaatta ttttaataaa agtattgaaa ttttgtttct ttagatatat
     4801 actctagtca tagaaatgaa gacggataca gaaaaagagg gataatatgt ttggcttttc
     4861 aatatttatg aacgattctt tgtcagaaga gaagaaacag tatatccgcc aaatggcatc
     4921 caatggcttt gtcggtattt tcacttccat gcatatacca gaagatgatg tttcggcata
     4981 tcaaaagcga ttgatcgaac taggaagcga agcgaaaaag aataaactag aattgatggt
     5041 cgatatttca ggagaggcat tggaacgagc gggtttttca ttagaagatc taacgccttt
     5101 gaaagcaatt ggtgtaacag gtttaagaat ggattatcat atttccaatc agcagattgc
     5161 cgactggtca catcaattga aaatcagttt gaatgctagc acgatcacgc ctaaagatat
     5221 cgatgaattg aaagaagcag aagctgattt ctcacagatt gaagcttggc ataattatta
     5281 tccacgtccc gaaacagggc tggacaaaga atggtatcag aaaaaaaatc aatggctcaa
     5341 aattcaaggt ctccttgtcc aaggttttgt accaggagat acagagttga gaggcccact
     5401 atatcaagga ttgccaactt tggaagaaca tcgagggatc catcctcttg caggcgctct
     5461 agatctatta gcaagtaaca cggatattgt ttacatcgga gatgcaggtc tgtcagaaaa
     5521 cgttcaggag caatttgctt ctttccaaaa agaacagaca gtcttgctcc atacggaacc
     5581 tgtagatgaa gaattttacg aatatatatt agggaagcat acgaatcggc aagacgatgc
     5641 aagagatgtc atacgaagcg tagatgcacg ttttcgagag attccgccta tcccagcaag
     5701 aaatacagct acgcgtatga aagggtctat tactttagat aacgaaaagt atttgcgcta
     5761 tatgggtgag atccaattaa ccaaatatga tttgccagcg gatgaaaaag tgaatgtagt
     5821 agctaaagtt atcaaagaag agctaccatt gatcaaccaa atcaaagctg gtatgaacta
     5881 tcaatttata agaaaagaag gaagataaaa atgagcaaaa tcaatttaga gaacctaaca
     5941 acggaacgca gaaaccaaga aacttttgga ttagatgaga tgagtatttc tcaagccgtt
     6001 caactaatga acaaagaaga tcagaaagtg gccgctgcgg ttgaaaaaga actgacaagt
     6061 atcgaaccag tgattgctaa cacgattgag tcctttaaaa aaggtggacg tctgatttat
     6121 atgggagccg gaacaagtgg aagactaggt gtgttagatg ctgctgaatg cgtaccgaca
     6181 ttcggtgtag aaccggaaat ggtcataggt ctaattgctg gtggagaaga agcaatgact
     6241 gtagctgtcg aaggggccga agattcttat gagctagggc aagaagatct aatcaagctg
     6301 aatttgacaa aaaatgatat agttattggt atcgcagcta gcggtcgtac accatatgtg
     6361 atcggtggat tagattacgc aaaatcgatc ggtgctgtca caggaacgat ttcttgcaat
     6421 aaacaagccg aaatcagtaa acacgcggat ttcccaatcg aagtcgatgc aggtccagaa
     6481 tttttgacag gctctactcg attgaagtca ggaacagcac aaaaacttat cttgaatatg
     6541 atttctacta tcagtatgat tggtataggt aaagtattca ataacttgat ggtcgatgtc
     6601 aaaccaacaa atgaaaaact aatcgaacgc tcaaaacgaa tcatcatgca agcgacagaa
     6661 gctgattacg aaacagctga aaagtacttt gaagaagcag atcaaaatgt taagctggct
     6721 atcgtcatga ttttgacaaa tagtgataaa gaggaagctc aagcaaaatt agctgaagca
     6781 aacggatttg taaaagggac cttatgaggg ggaaaagacc atggcaaaaa acgaactgac
     6841 tttggaagaa ctggcaaaac aaatttataa aggggcaggc ggcatgggca atgtagaaag
     6901 cattgtccac tgcatgactc gtgtgcgtat gaagatcatc aatgatgatc ttgtaaatgt
     6961 ctctgaatta aaagcgattc caggagtgct tggtgtagta gaagatgaac agcttcaagt
     7021 catcatcgga cctgggaaag tgaacaaagt agcacagcaa atggttgatt tggcaggcgt
     7081 gaaattagga gaaaaacttt cttctgtaag tacagctaca gcaaattcat cagcatctgg
     7141 aaaagaccgt gtaaacgaac gggcacaaga aatgaaagcg gcacaaaaat cgaaacaaaa
     7201 accatctaaa atcaaatcgg tattaaaaga catttctaat atctttgtgc caatgattcc
     7261 tgcatttgtc ggtactggga tcgtggcagg gattgccgca gttttagcaa acttagtcac
     7321 agccggaaca cttgatgcag caacatggca acaatatatc gatgtgatga acatcttgaa
     7381 aaacgggatg tttagctacc tagttatcta tacaggtatc aatgctgcac aagtttttgg
     7441 tgcaacacca actcttggtg gagttatcgg ggccgttgtg atgctgacag ggatgaatcc
     7501 ggaagcacca ctgaaaaact tatttactgg tgaagctctt gcagctggac aaggtgggat
     7561 cctaggtgtc atcttcgctg tttggctatt gtcaatcgtt gagaaaaaac tgcataaagt
     7621 agtacctgat gcggtagata tcatcgttac accaactctt tcgttgattg ccatcggtct
     7681 gattgaaatt ttcttcatca tgcctttagc aggtttcatt tctgatggca tggtcggtgg
     7741 gatcaactgg gtacttggcg ttggtggagc atttgccggc ttcgttctag gcacattatt
     7801 cttgccgatg gttatgttcg gactgcatca aattttgact ccaatccatg tgcaaatgat
     7861 tgatgaaact ggtcgtacgc tattgcttcc aattcttgcg atggctggtg caggacaagt
     7921 gggtgcagca ttggcattat ggattcgctg caaaaaagat aaagaattaa cagaaatgat
     7981 caaaggagct ttaccagtcg ggatcctagg aatcggcgaa ccattgattt acggagtaac
     8041 attacctcta ggacgcccat ttattactgc ttgtatcggt ggaggtatcg gcggggcagt
     8101 gatcggaatg atcggaaatg tcggggctat cgctattgga ccttcaggag ccgctttgat
     8161 cccattgatc tctgacggta aatggttagg ctacatcctt ggattattgg cagcttatgc
     8221 tggtggcttc gttgctacat tcttcttcgg tattcctaaa gaccgtttag aaaaagaaga
     8281 attagcgact gaaacagcca cagtaaatga agtgcctgaa aaagtagtac ctcaaacttc
     8341 agctgaaagt gtgactatcc tctcatcagt tgctgacgga acagttgttt ctttagataa
     8401 agcaagtgat ccagtttttg cacaaaagat gatgggtgaa ggctactttg tagaaccaga
     8461 aaacggacat atctattctc cagttactgg ggtagtcagc tctgtcttcc caacgaaaca
     8521 cgctattggg atcacaacgc caaatggttt agaaatcttg cttcatatgg gaatcaacac
     8581 agttgactta ggcggtacgc catttgatgt gaaagtaaca gaagggcagc aagtgacacc
     8641 tgatacgctt gtagcagaag ctgacttaga agcgatcaaa gcagcaggca aagaaacatc
     8701 catgatcgtc ttggtaacca atatggatcg tgtaaaaaac tttgtcttgg aaaaaacagg
     8761 taaagcaaaa gcaaaagcac ctgtgatgga tgtcaaagct taatatgata gaaacaatag
     8821 aaacgaaaga agaaactgaa atctattttt cagcttcttc ttttttatta tttattgttt
     8881 tctgctaaaa tctgataaat tcgtgtcaca tcagatggtg ttccccgtaa ttcataatct
     8941 gccaagaaag gaaagccaaa tgtttgtgca tattgtttgg ctgttagaca atactgatta
     9001 ttgaagtttt tatttccgct accgactaca ccaagacaaa gtttatgatt gtcttcatgt
     9061 tccagatatt cacgcatcgt ttcggtcaag atttctgtat caccgttatc taaaccattt
     9121 ccgccgtcta aataagtagg aacgaaagta aaaaatggtt ctgtttcttt cgtaaaatca
     9181 ctattttcat ggatttcttt taaagtaact tcaggattga caggatcttc tgcatgcatt
     9241 ttttcagcat attctgctag atgtttggca aaggcacgcg tatttcctga aatcgatata
     9301 tataaaatgt tcatggatca tcctccaatt ctttcctttt agtatagcat cttttttaag
     9361 ttttttggtg gtgtaatttg gaaagggact aggcgctttg cttgtataat gagaaaaaag
     9421 ccggtagaat tatatttcta ttaaagtttt tattaattaa gtaaaaataa aaaaggtttg
     9481 ttatactttt tgataaaagc ggttttattt tggaggatgc ttacatggat acagagcaac
     9541 agaaaaaagg aattggttta attccacttg ctgccttagt aattggttca gcgattggag
     9601 gtggcgtatt tggtattatg acggacatct ccggatcggc tggcggccca gctattttca
     9661 gttgggtact cgtaggaaca gcaatgttga tgctctcctt gtcgatcaat aatatcaata
     9721 aaaaacgtcc agatttaaaa ggcgggatct ttagttacgc agaagaaggt tttggccgtt
     9781 tcgctggttt tatcagcggt tggggttact ggctcacatg ctggttaggc aacgtagctt
     9841 ttgcaacatt gttgatgagc gcattaggat atttcttccc agtatttgaa ggtgggcaaa
     9901 atctggcatc gatcgtgatg agtacagtct ttttatgggg atatgcatgg ctagtgaatc
     9961 ggggagtcga aaatgctagt ttcgtcaatg ctatcgtcac attctgtaaa ttagtgccat
    10021 tatttttatt tattgtagta gctatcagca cgttttcgat ccaacaattc attgataact
    10081 tttctagcga cattatattg aatgcgaatg gacagccgat cccgttttcc agtcaagtat
    10141 tgagttgcat catggtgatg ctttgggtat ttgttggtat tgaaggagcc tctgtcgtag
    10201 gttcacgagc aaaaaaacaa tcagatgttg ggaaagcaac gattctaggg atattcggct
    10261 tgattattat ttacgtcttg gtttccatgc ttccatatgg gacaatgacg atgaatcagc
    10321 tgcaaacgat caaggcgcag cctgctatgg gctatgtgtt tgaaatgatg gttggtaaat
    10381 ggggagcagt cgtgatcaac ggcggtttga tcatttcttt agttggtgtt tggctctcat
    10441 ggacgatctt accgattgaa acaatgggaa acatggctga agacggactt ttgcctaaag
    10501 catggggaaa actcaacaaa aatggggcac caacttatgc tatcgtattg acgacgttat
    10561 gtacgaatgt ctttttactt tcattattat tcactgacag tgcttataac tttgcttaca
    10621 cacttggtac agcagctatt ttcttcacgt ggcttttctt aggattgtac caaatgaaac
    10681 tttcttatca gcggaaagaa tgggtccaat ttttagtagg gcttttagca agtgcctttc
    10741 aagtatgggc tatgctagtc gtagccttta atgaagtaat gcttgttctt gttcttttct
    10801 tgccaggcat ttatttttac ctcaaagcac gtaaggagca aaacgagcct gtacaacata
    10861 tccacttaga taagatttta cttataggtg tgacgatggt cggagtgatt gcaatggttt
    10921 tatttgccac tgggatgctt cacgtataga agtataaaag aagtataaag agaggttgtg
    10981 agcctgtcgt ttattcaaaa acatactagg aaaggagctg tgacacaaat gttgtcacag
    11041 ctcctttttc agaataaacg gtgtttaaga gacagccaat cggcaataag gctgaacgtc
    11101 tctttcacag cctctttttt gatgagtcat catacatttt ttcgcttaaa tacgagatat
    11161 cctaatgcta agaacaaaac gatgtaagct aaattaccga atagcatttg attagtagaa
    11221 agatgagtca attgcttcat gatctcttcg tttccaattt ggttttgcag attcaacata
    11281 ctgatcggat tccatttgat ccactcccat ttttggatga gtgcaataag gataccagat
    11341 atgattgacg aagcaaaata aaagacgatt cctgctgaga tggatgcgcc ggaactattg
    11401 ataaagcaag caatcatcaa cactaaactt aaaatcagcc ataaaccgat ataactaccc
    11461 aaagtataag tgaacaagct ttggatcagt gtttgaccag tagaaacctt ctccgtgaag
    11521 gaaatcgaag ggaaaagaat cagtttcatc aacacagtga caataatagc taaaaggtat
    11581 aaataaacag aataaataac taatgtgatc catttgctta ctaatatttc tcctcgagaa
    11641 tactttcgat aaaggagatt tttcaatgta ccatattgtg cttccatcga aatgatcgtg
    11701 cttgcagctg caatcatgat aaagacaatc caagatgtgg cactaaaaag ctgagcagtc
    11761 atttcaactg gatcaatgat cgtcgtgtat tttttggcta gaagtgctgt tcctatgagc
    11821 aaaacaacta atagaacaga taaaacagca gttgactttt tcttgatcaa tttaaataat
    11881 tcttgtttta caagcaatgt catttttctt cttccccctt tactgaatcc agaatttcca
    11941 atagagaaga ttccaaatca ttcgtcagat gctggacgtc tgtcacatgg atattttgtg
    12001 cagacaatag ctggatcacg gttcctaata attcttcgtt ttcttttaaa agcttgattt
    12061 tatcaggatc ttccaaagta tatccagctt ctgtcaagat cgttcgagca gctagatcat
    12121 ttgttgtaga gataacgatg aaatcttgcg ctgtttggtt caattcagcc attgtacatt
    12181 gtttgatgat ctttccttga ttgatcagga tcagctcatc gaccattttt tcaagctcgc
    12241 tgagtaagtg actggagatc aagaaagagg tgccctgagt agccaagtcg gtaatgattt
    12301 ttcgcacatc tcttgttgct tgtggatcca aaccgttcat cggctcatct aaaataacga
    12361 tagacggatg gttcagtaag gcaagagcaa ttcccaattt ttgtttcatt cccaacgagt
    12421 aggatttcgc ttttttgtga atgtactttt ccatcttgag ctgattcacg atagtgtcga
    12481 tagcagatac atcgttggtt tcagagaata acttcagatg atcatagcct gttaagaatg
    12541 gatagatacc cgggtattcg atcaatgctc ctacttgttc caacccttta tgactagagg
    12601 gagaaatctc attttcacca atacagattg ttccatctga gtaatggatc agtcctaaaa
    12661 tagctttcat gatcgttgtt tttccagcgc cattaggacc tactaatcca acgattttgc
    12721 caggggataa gctaaaactg acatcgctga gggcttgttt gtgaccaaag ttcttggata
    12781 attcttggac cgaaagactt ttcaaaaata ttcactcctt ttctatagat agtacaagca
    12841 tacacctgat gagtggagaa gtcgctaggt cattggactt atttcttatt ttttcataaa
    12901 aaaattgaat attaaataaa aaggcttatt ttttctgatc agtacttatt ccaagtgtct
    12961 agtggccgct agtaatcttg atgccaatga tactgatgag tagcatcccc acaaagaacc
    13021 aagtggaaag attcagctta tcatgaaaca agaccacacc aattatgacc gaaccaactg
    13081 cgccaatccc tgtccagata ggataagaga gactcatcgg caaacttttg attgcctttg
    13141 acaaaaagta aaaactagca atcatgccaa tcactgtgta aaaagaataa ttgattttag
    13201 aaaaaccatc actccatttc atcatcgttg accagaatac ttccaaaatt cctgctatga
    13261 ttaattccat ccatgccata cttacattcc tccttaacta taaaaaaata cacaaaaaga
    13321 ggaaaatcat cccttcaaag acctaatgga atgatcatcc tctttatcct acccggtggc
    13381 agattgtata tctttttctg tagtatagca cacctatcca gatatcggca atctcatact
    13441 ttttagtaaa gtgtaccaag cactgacgga tatatcgtta gcggaaaaga agatgaatgc
    13501 tttgcaattt catagaaaga agttgttatt tattaatcat ttaaaaaaaa cagtaactag
    13561 accttatttc tatatattat cgggcaataa attaaaaaaa gctttacctt acaataagta
    13621 agataatttt tgtgataaaa aaat
//
LOCUS       DACENJ010000059        13636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.059, whole genome shotgun sequence.
ACCESSION   DACENJ010000059 DACENJ010000000
VERSION     DACENJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.059"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..499)
                     /locus_tag="IW748_001257"
     CDS             complement(<1..499)
                     /locus_tag="IW748_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104176.1"
                     /translation="MCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIAEFIGSFRG
                     NVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMNDGGSKPQL
                     TVRDRI"
     gene            complement(1066..2001)
                     /gene="whiA"
                     /locus_tag="IW748_001258"
     CDS             complement(1066..2001)
                     /gene="whiA"
                     /locus_tag="IW748_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1104177.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2035..3033)
                     /locus_tag="IW748_001259"
     CDS             complement(2035..3033)
                     /locus_tag="IW748_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1104178.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3030..3914)
                     /gene="rapZ"
                     /locus_tag="IW748_001260"
     CDS             complement(3030..3914)
                     /gene="rapZ"
                     /locus_tag="IW748_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1104179.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4049..4780)
                     /locus_tag="IW748_001261"
     CDS             complement(4049..4780)
                     /locus_tag="IW748_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1104180.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(4784..6049)
                     /locus_tag="IW748_001262"
     CDS             complement(4784..6049)
                     /locus_tag="IW748_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1104181.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(6312..9128)
                     /gene="uvrA"
                     /locus_tag="IW748_001263"
     CDS             complement(6312..9128)
                     /gene="uvrA"
                     /locus_tag="IW748_001263"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1104182.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9138..11132)
                     /gene="uvrB"
                     /locus_tag="IW748_001264"
     CDS             complement(9138..11132)
                     /gene="uvrB"
                     /locus_tag="IW748_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1104183.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11391..12893
                     /locus_tag="IW748_001265"
     CDS             11391..12893
                     /locus_tag="IW748_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880041.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1104184.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIEKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            12895..13632
                     /locus_tag="IW748_001266"
     CDS             12895..13632
                     /locus_tag="IW748_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104185.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT         3941 a         2946 c         2341 g         4408 t
ORIGIN      
        1 caatacgatc acggactgtg agctgtggct tgcttccacc atcattcata aaatatccag
       61 cagagccact acttaaagtg attgttacat cttcactttc tattattaag aattcataat
      121 gaaatgcttt caactctcca ctattattta aaagttttat actccaactt cgtctaggtt
      181 gagttgtcac attccctcta aaacttccta taaattctgc aatcaccgta tcttcaatca
      241 aaattctttc ctcatctatt tcatattcaa gactaaacgg aaattctcca tatgtaatct
      301 ctggcaatgg tggtgctgta gttctccaaa taaatacaat aactgtgaat agaaaaaatc
      361 caacgatcga tataaatgtt atgagacctg ctcgaagtaa aacccgatat aataaaaatg
      421 ctgtatttcg ctttgctata tctagaaaaa taaaaagtac tgcaatcata atgatagtaa
      481 aagttgtaaa aaagcacatt ttttgccctc cctttataaa catcgattct caaattctaa
      541 accaaatctt gatagttcaa gttcaggtat aacgtatacc aaccatacat cccaattact
      601 atttatgaac ttatgattat cactgaataa atcactccat ccccttgtta aaacttctac
      661 attaattaca cctggttgtt ttcgactagc taatccaatg aacttatcaa ccatccttat
      721 ccaccaacca cgagaatctt gaccagaagt aagaaaaaca gttctcaagg ttgatcgagc
      781 agttccttcc agtttttaat taatctccag ttatagataa atacttgatc ctatctatat
      841 caggacttct atattaagca aaagtttatg tataaaacta aaatcttcat tcagttgtct
      901 ccccgctttt tatacgattg tattaaaaaa caatagaagc ctttcaacat tatgttactc
      961 cagtaaagtt ggaattatag tttgacggaa gaaaagcaag tatagctttt aaaaaaaact
     1021 gatacattcg catctcctgc ttcatgtatc agttttttct ttggattaaa ctgcttcttc
     1081 acgcagtttt tccgcaaatt catttatttt gcgaattcgg tgattaattc ccgattttga
     1141 tattgcacct gagggaatca tttcccctaa ttctttcaag ctgacctctg gatgttgaag
     1201 ccgaagttct gcgatttcct gaagtttttc aggaagggca tgtagaccga cctttgcttc
     1261 gatcaattcg atgttttcga tttgtttaga agccgcatca atcgtcttgt tcagatttgc
     1321 tgtctcacaa ttgactagac gattgaccga atttctcata tcccgtacga ttcgaacatc
     1381 ttcaaatcgg agcattgagt tggttgcacc aatcaatgtt aagaaatcag caattttttc
     1441 tgctcctttt aaatacgaaa tatagccatt gcgccgttca agcgttcgag catgtagacc
     1501 gtaataattt aacatatcgc aaatgtcttg gttatgctgt tcataaatag aaaatatttc
     1561 caaatgataa cgactcgttt ccggattatt gactgaacct gaggccataa aagcaccacg
     1621 taagtaagaa cgcatttttt gcgaatttcc cataatctcg tccgaaacgt gtccattgaa
     1681 catcatacca tccatgatat caagatccat caacacatct tttgtgcctt gcttcaaccg
     1741 gacgatatag acattgttct tttttaattt cattttttta cgaacgagta gctcacttcg
     1801 tacctgataa tgatctttca aaagagaata gatacgtctg gcaattgcag cattttctgt
     1861 ttggacgttc aaaataaact gctggttcac aatacctaaa gagccgttca ttcgcagcaa
     1921 agcagctaat tccgctttcg catgttctcg atgaacttcc aaaccagtta gttctttctt
     1981 cacatctgca gcaaatgaca tcctattctc ctcctttctc caacaataaa ttgcttaata
     2041 ttttgaaccg aaaactaaac ggaacaattc ttctactact ttgtctccat cgtggaagac
     2101 accaccatct ttcaattcta aaaagtcggt ggaaatcact cgacatcctt catttctcaa
     2161 tccgttgaag tcgtgtgcga cttgtactag atactcatca tagatctctg ggtccatata
     2221 atcttcaggc actttttccg tattgaccaa tactgtatcg ataaaaggct gattcaaatg
     2281 cttgtgtaaa acacgtatat gatcggcatc tgtgaaatgt tccgtttccc ctttttgggt
     2341 catgatatta cagatataga cggtttcagc agttgtcttg agcattgctt cgccgatttc
     2401 tggtatcact aaatttggaa ggatactggt gaataaactt ccgggtccca aaacgaccat
     2461 gtcggcgtcc ataattgctg aaacgacttt tcgagccgct ttggcttcac ttttatcatt
     2521 tgtgttgcag acatacacac ggtcaatggt tttacggttg atcgcgattt tagattctcc
     2581 tactgcagtc gttccgtctt taaaaacagc atgcaaggtc aaaggagttt ctgaagaagg
     2641 ataaacatgt ccatctacat gcatcatttt tgctaataat tgaatcgcct cataagtgct
     2701 tccccgcatc tcggaaactg cggcaatgat cagattaccg atcgtatgat tggctaaaaa
     2761 ctgatcttcc tttttgaaac ggtattggaa aatatcttca tagaattgtg gcatatccga
     2821 taacgccact aagacatttc tcaagtcacc aggaggagcc atagcgatcg attctctgat
     2881 tgcaccgctg cttccaccat catctgcaac agtcactact gctgtgatat ctgctccttg
     2941 atttcgtaaa cttttcagga taactggcag accagtgcca ccgccgacca ctacgatctt
     3001 tggttttctt atccggtagg ttttcatttt cacgaccggt tcaccgtctc tttcctttta
     3061 tccttgtccc gatgtgtcac attgacttta tagtttttcc ctaaagcatt tgccaaacgt
     3121 tcagtcaatg ccacagaacg gtgttgaccg cctgtacagc cgattgcaac tgtcaagctg
     3181 ctttttcctt ctttaatgta tccaggcaat acagtttcca gaagatccaa aaatttatgg
     3241 tagaatgatt gcgtttcatc aaaggacatc acataatcat agactggctg atcttttccg
     3301 gtcaatggtc ttagttccgg gatataatga gggttgggca aaaaacgcac atccatcaca
     3361 atatccgaat cgatcggcaa cccgtattta aaaccaaatg atacaaattc gatccgaaat
     3421 ccttggtctc cctttgattt gaatgactca ttgatttttt cacgtaattg tcgtggtgtc
     3481 aatgttgtcg tatcaatgat cacggaagct tgggctttca agtcgtctag tatcgcccgt
     3541 tctttacgta tcccttcagt gatcaaaccg tccatcgcca ttggatgtgc tcgtcttgtt
     3601 tctttgtagc gagccactag ttcttcgtct gaagcatcta aaaataagat agaagtgtcg
     3661 atgaagttcg tattttcgat ttcgatcagc atgttttgga tttcttcaaa gaaggaacga
     3721 gaacgcaaat ccactcctaa agccattttt gtgactttac ctgattcttt gatcaattcc
     3781 cagaattttg gaatcaatcg aggcggcata ttatcgatac aaaaatagcc catatcttca
     3841 aaactttgga tagctactgt tttccctgca ccactcatac ctgttatgat gactaattgc
     3901 aaattatctg tcatatttac gaacccctct cacattgtgc ataataaaat tatatcatcc
     3961 cgggcgtatc attaccagtt ttctttttct tttacaaaaa gatttggacc aaatatcttt
     4021 taggatattt gccccaaatc caattttttt acttttccga tgctgtatcc aatgcctcgc
     4081 ttctttcagc taatgcacgt tcgatcgttc gatccgctaa aatagattga gcatcgatca
     4141 ctgggtaatg attatcttca gcaaattcat gcatcaaaga taattctgtg caacctagga
     4201 tgactttttc acagtttagg cgctctaccg cttcctctaa gatttcgtaa tagagctctt
     4261 gattcaaata atccgattcc ttgatctcat gatagatcag gtaattgatt ttttcttgaa
     4321 gcgcagtatc aggaatcact gtttcgaaac caagattttt gacttcgcgt tcatagattc
     4381 cagctttcat acttccttct gttcctagaa tcgctactct ccctgtcgta tgctgacgga
     4441 caagttcatt tgctgcttct cttggcatat gcaagatagg aatatccgtt gccgcttgta
     4501 actcttcaaa aaagtagtgt gcggtgttac aagtcaacac aatgaagttt ggtcgcaata
     4561 aattttgttt ctcaatatca tccagcagaa acggcattgg attttcttct gatcgatcta
     4621 aaatataagc tgtacgatca ggaactgttg catgattgaa taagacataa ttcaaatatt
     4681 cttgatcttt tgtagctttc gttcggtggt tgatcaagcg aacaaaactt tctgtcgcca
     4741 tcgtgcccat tccgcctaaa atactgaaaa aattctccat gaactagcct tctttcacat
     4801 gaaaatactt tttgaatctt ggaatatagt tatggaacat atatttcatc aacaaccagc
     4861 gcttgattga tcggtctttt tcataaaata cagtagtacc gtaacgattt tctttgatca
     4921 tttgctcagc tagttttttg tcttcatttt cccgagcata ttctaaaaag atttttttgg
     4981 gcacacccat ccataatctt gctttatctg attgattcgt tccataggtc gtttcaacaa
     5041 acggtttgtt gaacactcga tcttctgtga caaaacgagc cagattcaag ccgttcaatg
     5101 tcacaaagaa actgctacga ccttgtctca aattgatttc aaataattta tattctccat
     5161 ctctaggatc atacttcata tcgaaattgg caaagcctgt atattcgatt ttttccaaaa
     5221 atgccttaat ggtttgatag attttttcgt tataatctgg catgatcaca acgtaattac
     5281 caatcgatgc tggtgtagga tcttctaata gtggatgacc aaggcacatc atccgaactt
     5341 gatgatcttc atctacatac gcatttagca cacgcatatt actatcatca cccgggataa
     5401 aatcttgtac aatcatttca cttttataac cggcttcgta tatccggcct aaaattaaat
     5461 cgaactcttc tctagtctct aaaataaaag ctttttttct tccttcaaat tggacagaaa
     5521 gatactccac actgtttgca ggtttcaaag caactgggaa ttcaaaaggc aattcctgtt
     5581 ccaaatgtcc atttactaac atttcttctc tgacgatcaa tgttttagga tacggaagat
     5641 catattcttc acaaacttca taaaagctta ctttattgat cagcctttca aataaagaat
     5701 aatcaatata tggacagata aaatattctg acagctcttg tttatgttgg gagatcaatt
     5761 ctgcatatcc atcaccacaa gcaatcaaaa gatatttttt actcttatca gaatatcttt
     5821 cttttgccaa acgaagcatc gtctcgataa atacagggtc tttatcaaat cctggaataa
     5881 cttctacatt gacaatttta ctgtagcggg taggggctaa ctgatccgaa gcatatgctt
     5941 ggcagatttt gccgtatgct tcattaaaag aacgtgccat accataaacg ttcatgtcgc
     6001 ttcctaataa tatagggata aattcttcat tttcaatact gttcatcatt atctctcact
     6061 tactcataat ctcttgtttt tcttttccaa ctacacaagg aatatcatac cataaagaaa
     6121 aaagcaatca aaggaaactc ctccgattgc tcaattcttt tttgaacgga ttgaacttat
     6181 aggaagtttt tatctatacc aatcagtgta gaaagaacta ccatgtaatg attctagaac
     6241 actgatatta ataaaacaag caaaaattca tttttttatg tttttttaac tatctcagct
     6301 atctatcctc tttataccat tatttttttc aaatagtgac ctgtataact ttcttttacg
     6361 ttaatgattt cttctggtgt tccagtcgca aggatcgttc ctccaccttc tccaccttct
     6421 ggtcctaaat caatgatata atctgctgtt ttgattacat ctagattgtg ttcaatcact
     6481 aaaactgtgt taccagcatc tactaatctt tgtaatacat gcaacaagcg ggcgatgtca
     6541 tcgctatgaa gtcctgtcgt tggttcatct agtatataga aattctttcc attagagatt
     6601 ttgtgcaatt cactggcaag tttcatccgt tgtgcctcac caccggacaa tgtcgttgct
     6661 ggttgcccca tcgtcacata acctaagcca acatcaacaa tcgtttgcag tttgcgatga
     6721 atctttggaa tgtgcttgaa gaattctaca gcatcctcta ctgtcatttc caaaatatca
     6781 gcaatgcttt ttcctttgta atgcacttct aatgtttcag aattatatcg tttaccatga
     6841 catacttcac aaggaacata gacatcaggc aaaaagtgca tttcgatctt aataattcca
     6901 tccccgcgac aagcttcaca acgaccgcct tttacgttga aactaaaacg ccctttctta
     6961 taaccccgca tttttgcctc gttcgtttga gcaaataacc cacggatatc atcaaataca
     7021 cttgtataag tcgctggatt acttctcggc gtccggccga ttgggctttg atcgatatcg
     7081 atgatctttt cgatactttc gtagccggaa attgtcttga atttacctgg cttagcagaa
     7141 ttcttattta atttttgcgc taacgatttc tttaagatac tattgaccaa tgtactcttt
     7201 ccagaaccag acacacctgt gactgctaca aattctccta atggaaattc aacactaata
     7261 tttttcagat tgttttctga agcaccagtg atcttgattg ctttcccatt acctttacgg
     7321 cgttcttttg gtacagggat tgattttttc cctgaaagat atttccctgt caaagaattt
     7381 tcatcattag ctacttcttc aggagtgcct gcagccacga tttgtccgcc ttgttctccg
     7441 gccccaggtc cgacgtcaat caaataatct gctgcccaca tcgtatcttc atcatgttcg
     7501 actacaacga gggtgttgcc aagatcacgc attttcttta atgagccaat cagtcgatcg
     7561 ttgtcgcgtt gatgcaatcc gatcgatggt tcatctaaaa tatacaaaac tccagaaaga
     7621 ttcgatccaa tttgtgtagc caatctgatt cgctgcgctt ctcccccaga taatgttccg
     7681 gaagcgcgac tcaaagttag atagtctagt cccacatttt ccaagaatga aagtctgtca
     7741 tttacttctt ttaaaatcgg tttggcaatt actttctctt gttcagataa cgttaagttt
     7801 gagaagaaat ctactgcttt ttgaatagac aactcatttg tttgcccgat attcgttcca
     7861 ttgatttgaa cagatagagc ttggggattc aatcgataac ctttacatgt ctgacaagtt
     7921 aattctgtca tataaagacg catttgctca cgcgtgaaat cactgttcgt atcatgataa
     7981 cgacgctcga tattcttcaa taccccttcg aaagtcgttt ctacatcgcg aacgccacca
     8041 aaatcatttt cataatggaa atggaaaaga tcatctgtcc cattcaagat gatctcctga
     8101 tgctcttccg gtaattcttc gaatggtgta tctaaatcga taccgaaact ttcacaagcc
     8161 tgcgctaaca tttgaggata atactgagag ctgattggat tccatggaac gatagcacct
     8221 tgactaagtg tcttcgttct atctggaatc actaaatctt gatcgacttc taatttgaca
     8281 cctaaaccat cacagtctgg acaagctcca aaaggcgcat tgaatgagaa cagtcttggt
     8341 tctagttcgc caacagtaaa tccgcaataa ggacaggcat agtgttcgct aaacaacatt
     8401 tcttcttcac cgatcacgtc aaccagagca tagccgtctg ctaatcgcag ggccgcctct
     8461 aaagaatcaa ataggcgaga acgaatccct tctttcacaa tgatccggtc gatgacgatc
     8521 gcgatatcat gttttttgtt tttctccagc tcaggcactt cacttaaatc atagatctca
     8581 ccatcgactc gaacgcggac gtatccctct ttttggattc gttcgaatat ttttttatgt
     8641 tgtccttttt tctttaccac gatcggtgcc aacacttgga tctttgtgcg ctcaggcaaa
     8701 gccattactt gatctgccat ttgatctact gattgactgg taatctcgat atgatcgttg
     8761 ggacaaatcg gatgtcctac acgtgcaaac aacaagcgta aatagtcatt gatttccgtc
     8821 acagtaccta ctgtcgaccg aggattttta cttgttgttt tctgatcaat agaaatcgcc
     8881 ggactcaatc cgtcgatgct atctacgtcc ggcttatcca tttgtcctaa aaactgacga
     8941 gcataagcag aaaggctctc tacgtatctc cgctgccctt cagcgtacag cgtatcaaat
     9001 gctagggaac tttttccaga accggacaaa cctgtaacga ccacgaattt atctcgtgga
     9061 atcgttacat ctatattttt caagttatgg gcacgtgcac catgaatcgt aattttatcg
     9121 ttagccatgt tgtcctccta ctttgaagct tttaattcta aaatcatatc tcgcacattc
     9181 gcggctgttt caaaatccaa agcttttgcc gcatctttca tttctttttc taatttcatc
     9241 aataacgtgt ctttttcttc cttcgtcatt tcttcgtacg agacttgtac cgcttctttt
     9301 gtatcatctt ccgattcttt cgtaatagaa atcaaatctc ggatttcttt aatgatcgtt
     9361 tttggaacga tcccatgttc ttcattgtac ttctgctgaa tcgtacgacg tcttgacgtt
     9421 tcatccatcg ctagacgcat agaatctgtt actttgtctg cgtacatgat tactttacct
     9481 tcttcattcc gagcagctcg tccaatcgtt tggactaagg aacgttcact acgtaaaaag
     9541 ccttctttat ccgcatccaa gatagcaaca agggaaactt caggtacgtc taatccttca
     9601 cgaagcaaat tgattccaac taacacatca aattcaccta atcgaagatc tcgaatgatt
     9661 tctgttcgtt ccagcgtttt gatatcacta tgcaaatatt tgactttgat ccctaattct
     9721 ttgaaataat ctgtgaggtc ttctgccatt tttttcgtca atgtcgttac aaacacccgc
     9781 tggtcttttt cgactcgttc atttatttcg cctactagat catcgatttg tcccatgatt
     9841 ggacgaactt cgatcactgg gtcaagcagc cctgtcggac ggatgatttg ttggataacg
     9901 gtatctgttt gttcatgttc atatggtcct ggcgtcgcag agacataaat gatctgattg
     9961 acatgttttt caaactcttc taaccgaagt ggccgattgt ctaaagcaga tggcaaacgg
    10021 aaaccgtaat ctactagcat ctgttttctc gctcggtcac cattatacat tcctctgatt
    10081 tgaggcatgg ttacgtgcga ttcgtccgcc acgatcaaaa aatcttcagg aaagaaatcc
    10141 aatagcgtat aaggcggttc accttcttta cgtccgtcca tatgccgaga ataattttca
    10201 attcccgacg tgtagcccat ttctaacatc atttcaatat cgtaattggt tcgctgttct
    10261 aaacgttgag cttccagaag tttgttttca tttcgcaaga cagttaaacg ctgttctaat
    10321 tccgctttga tattcgctac ggcatgctcc atatgttcat cattcgtcac aaagtgagtc
    10381 gccgggaaga ttgccacgtg ttccgtttct cctaaaactt cacctgtcag tgcattgact
    10441 tctcggatac gttcgacctc atctccaaaa aactccacac gtaaggcacg ttcatctctt
    10501 gaagccggaa aaatctccac gacatcaccg cgcactcgaa aacgaccgcg ctgaaaatct
    10561 atatcatttc tttcgaattg gatactgact aagtcacgaa tcaactgatt gcgatctagt
    10621 tctgctccct gtcgaatcga aactacttgt ttttggtact caaaaggcga acctaatcca
    10681 aaaatacatg aaacggatgc aatgacgatc acatcatttc tttctaataa tgaactagta
    10741 gccgaatgtc gtaatttgtc gatctcatca tttacacttg aatctttttc gatatacgta
    10801 tcgcttgaag gcacataagc ttcaggctga taataatcat aataagaaac aaaatattca
    10861 acagcattgt tagggaaaaa ttccttgaat tccccgtaca gctgaccagc caatgtttta
    10921 ttatgagcaa taatcagtgt tggtttattc actttctcaa taagattgga aatcgtatat
    10981 gttttccctg ttccagtcgc acctaataag atttgtgctt ttttcccgcc gactacgcca
    11041 tccactaatt ggttaatcgc ttcaggctga tcaccagccg gttggtattt cgagacaagc
    11101 tcaaagtgtc gggaggtatc tctctcaatc attcttcgtc ctccttttca ttcattcctt
    11161 ttcattttac gaccaataaa aagaaaagga aagcaaaggc attccttact aaaaaattat
    11221 accacaccga acatactttc gccacttttt tatcttatta tacatgttac gttttataac
    11281 ataaaatgtt agaaactcgc atttttttca ttttttcgag cttacagtct tgtctgattc
    11341 agcacttttc gcctataata ataaaatatt ggaaatttag gaggttgatt atgaaaagaa
    11401 agcatttatt tcactcattt gttttcttaa tgacaggact tgtgacactt gcgtttggtc
    11461 atgttgcaca agctgacaac actacaacgg cagagtcaag cacaagcgag acaatgcaaa
    11521 aaatagagcc caaaaaagac gtatacgtaa ttgccagtga ctctgctttt gctccatttg
    11581 aatttcaaaa cgcaaagggc gagtacgaag gcatcgatgt aaaattgatg gaaaacatcg
    11641 ccaaactgca aggatttaac atccgaatgg actaccgtgg tttctctgct gctttgcaag
    11701 ctttggaatc aggacaagca gacggaatga ttgccggaat gactgtaaca gatgaacgaa
    11761 agaaaagcta tgatttttct gattcttact ttgatagcgg gatccaatta gctgtcaaaa
    11821 aaggagatac ttcgatcaag tcttatgaag atttgaaagg aaaaacagtc ggagcaaaag
    11881 ttggtaccga aagtgctgaa tttttaagcg accatgaatc tgaatatggc tattctatca
    11941 aatattttga tgctgctgat caattgtacg acgggttaaa agttggcgca atcgatgcaa
    12001 tgatggatga ttatccggta atcggttatg cgatcaaaca aggacaagaa ttggaaacac
    12061 cgatcgaaaa agaatctggc ggaaaatatg catttgccgt taaaaaagga caaaatcctg
    12121 agcttcttaa tatgttcaat gaaggattga aagaattaaa aagaacaggc gaatacgata
    12181 agattatcaa agaatatgta gcggatggtt cagaaacttc tagtgagtct tccactgctg
    12241 atgaatcaac atttcttgga cttatccaaa acaactatca agcattgttg aaaggacttt
    12301 ggagaacaat tctcctaact ttgatctcct ttgctttggc tatagtaatc ggtgtgattt
    12361 ttggattatt cagtgtagca ccaatacgcg ctcttcgcac aattgctagt atttatgtcg
    12421 atatcatccg aggaattcca atgatggttc ttgcgttctt tattttcttt gggttgcctg
    12481 gtatcgttgg atttacgatt cctgacttcc ttgctggtgt tatcaccttg actcttaacg
    12541 caagtgctta tatcgcagag attgttcgag gcgggatcaa cgccgttcct gtcggacaaa
    12601 tggaagcttc cagaagttta ggtctttctt ataatcggac aatgcaaaaa atcattttgc
    12661 cacaagcaat acgcatcatg atcccatcat tcgtcaatca gttcgttatc tcattaaaag
    12721 acacaacgat catttccgca atcggtgtag tcgaattgct gcaaactggt aagatcattg
    12781 ttgcacgtac aacacaaagt acgtatgttt acttgatcat tgcgatcatg tacttgattt
    12841 tgatcacagc attgacaaaa ttagcaaaag ttttagaaaa gaaggtgaag taagatggca
    12901 gaaaaaatct tagttgagca tttagtcaaa aaatacggcg ataacacagt attaaacgac
    12961 atcaatgtat ccatcaacga aggagatgtc gtctgtatca ttggcccttc aggttcagga
    13021 aaaagtacat ttttgcgttg cttgaatcgt ttagaagaac cttctgacgg tgaaattatt
    13081 attgacggcg cccacttgat ggataaaagc acagatatca acttggtgag acaacatatc
    13141 ggaatggtat tccagcactt taacctattc cctcatcttt ctattttgga aaacatcatt
    13201 ttagcaccga tggacctgaa gaaagaatct agagaagaag cagagaaaaa agcaatcaaa
    13261 ttactgcaga cagttggtct agaagataaa aaagatatgt atcctgaaat gctttctggt
    13321 ggacaaaaac agcgtgtggc tattgctcgt gctctagcga tgaaccctga catcatgctt
    13381 tttgatgagc ctacatctgc actcgacccc gaaatggtcg gtgatgtctt gaacgtcatg
    13441 aaaaagttag cagaacaagg tatgacgatg gttatcgtta cgcacgaaat gggcttcgct
    13501 aaagaagtgg ccaatcgtgt catgttcatt gacgacggaa actttttaga agacggaacg
    13561 cctcaacaaa tctttgaaaa tccgcaaaat gaacggacaa aagatttctt gaataaagta
    13621 ttgaatattt aataaa
//
LOCUS       DACENJ010000060        13342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.060, whole genome shotgun sequence.
ACCESSION   DACENJ010000060 DACENJ010000000
VERSION     DACENJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.060"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1097
                     /locus_tag="IW748_001267"
     CDS             <1..1097
                     /locus_tag="IW748_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1104186.1"
                     /translation="LEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLARSVKL
                     HYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANLFLIF
                     YLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAAVPFK
                     KGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAGYQMT
                     HSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLLFSLC
                     MRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYGGTSY
                     LILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1189..1944
                     /locus_tag="IW748_001268"
     CDS             1189..1944
                     /locus_tag="IW748_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1104187.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2002..4041
                     /locus_tag="IW748_001269"
     CDS             2002..4041
                     /locus_tag="IW748_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1104188.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4150..5301)
                     /locus_tag="IW748_001270"
     CDS             complement(4150..5301)
                     /locus_tag="IW748_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1104189.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5455..5985)
                     /locus_tag="IW748_001271"
     CDS             complement(5455..5985)
                     /locus_tag="IW748_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104190.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6084..6434)
                     /locus_tag="IW748_001272"
     CDS             complement(6084..6434)
                     /locus_tag="IW748_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1104191.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(6591..8120)
                     /gene="ahpF"
                     /locus_tag="IW748_001273"
     CDS             complement(6591..8120)
                     /gene="ahpF"
                     /locus_tag="IW748_001273"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1104192.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8130..8693)
                     /gene="ahpC"
                     /locus_tag="IW748_001274"
     CDS             complement(8130..8693)
                     /gene="ahpC"
                     /locus_tag="IW748_001274"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1104193.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(8837..9499)
                     /locus_tag="IW748_001275"
     CDS             complement(8837..9499)
                     /locus_tag="IW748_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1104194.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            9683..11329
                     /locus_tag="IW748_001276"
     CDS             9683..11329
                     /locus_tag="IW748_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1104195.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11320..12402
                     /gene="uxuA"
                     /locus_tag="IW748_001277"
     CDS             11320..12402
                     /gene="uxuA"
                     /locus_tag="IW748_001277"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1104196.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(12510..>13342)
                     /locus_tag="IW748_001278"
     CDS             complement(12510..>13342)
                     /locus_tag="IW748_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1104197.1"
                     /translation="TEYEYIDFFIDLSVDILRPNLPICREQRILVNILARLSITKIES
                     EETSLEQYKLLKKANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFARNKLYNGF
                     LNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVE
                     DLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVI
                     SDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS
                     "
BASE COUNT         4396 a         2451 c         2529 g         3966 t
ORIGIN      
        1 ttttagaagt atacagtgcc agttcttatc gtttattgca agccgatgaa aatacaaaaa
       61 gtctattatt gcgtcaacta attttcatat ttttgagttg gagcgtgatc ttcttagctc
      121 gttcagtcaa actacactat ttacttcacc ctaaaatagc aggatacggt ttagccttat
      181 cgattttctt tttagtatta gtaagaatag ggatattcgg tgtcactgtc aacggcgcac
      241 aacgttggat ctctctgttt ggcattcaat tccagccttc tgaactggca aatctttttt
      301 tgatttttta tttaagctgg ttttttcgtg acggaaatag tagcccaaaa gatctaaaaa
      361 aaccattcct gattacagta ggtataactt ttctgatttt atttcagcca aagattgctg
      421 gagcattgat gatcctttcg attgcgtggg tcatattttg ggcagcggcg gttccattta
      481 aaaaagggat ctatctaatc gttacttttt ctgcattgct gattggagca gcaggcgggg
      541 tattatattt aggaaataaa ggttggcttc cacagatgtt taatcatgct tatgaaagga
      601 tcgcgacttt aagagattct tttatcgata gtcatggtgc gggctatcaa atgacccatt
      661 cgttttatgc actatataat ggtggcatat ttgggcgggg attaggaaat agtatcacaa
      721 aaaaagggta tcttccagag acagaaacag attttatttt ttcgattatt acagaagagc
      781 ttgggctaat cggtgcattg tgtgtgcttt tcttattgtt ctcattatgc atgagaatct
      841 tttgtttaag cagtcgctgt aaaaatcagc aagctggatt gtttctttta ggattcggaa
      901 ctttgctatt cgttcagacg ataatgaacg tgggaagtat cgcggggctg atgccaatga
      961 ctggtgtgcc attgccattt gttagctacg gaggaacaag ttatctgatc ctttctttag
     1021 gaatcggaat cacgttgaat atttcttcaa agatacaggc cgaagagttg gcgctctatc
     1081 gacctgaaaa acaataaaga gaaactgaga aactctcagt ttgaattatc ataaagtaca
     1141 atcttttatt tttcaagcga gaggttcttt tagaaagagg aattcgcaat gaaagtatct
     1201 caaaaaatca tattatctat tttgttactg cttacattag tagttggcta tttttcgatt
     1261 gagtttatcc atggattttc gtctgcaaaa cagagttcaa ctgtgaaaaa ggtagagccg
     1321 aaaagtatcc ctaccacact aaatgtggct ttgattggtt cagatgctcg ttcgaaagac
     1381 gaaaacggtc gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca
     1441 aaactgatct ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag
     1501 ataaatgcag cttactctta tgggggagtt gatttattaa atcaaacatt aaaggaaaat
     1561 ttcaaatttg aagctcccta ttatgcaagt atcacatttc aagattttat cgattgcgtt
     1621 aatgaactgt ttcctggtgg agtcaagatc gatgcagaaa aatctctaga tttagatggc
     1681 gtatatataa acaaaggaga gcaagtgatg gatggtaata ctttactcca gtatgctcga
     1741 tttcgggaag acaaagaagg agacttcggg aggatcagaa ggcagcagca agtgatcaaa
     1801 gctatctctc agcagctgaa agacgtaact tcaatattca agctacccaa agcggttggg
     1861 aaattacttg gtagtatcca aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg
     1921 gatttcctca aagacatatg ttagaataaa caggtataaa tagtgaaata aaggaatgac
     1981 aagcaagaga aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc
     2041 gcttatattg ccggcgcagt gatcttaata gcagctgcgg gtggcggtta tttttactac
     2101 cagcactatc aagaaaccca agcagtagaa gctggagaaa agacagttga gcaatttgtc
     2161 caagctttga acaagggaga ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca
     2221 aataaaagtg cattatctga aaaagagatc ttagataaat accagaatat atacggtgct
     2281 gctgatgtca aaggacttca gatatcgaat ctaaaagtag ataaaaaaga cgattctact
     2341 tacagttttt catataaagc aaagatgaat acctcattag gtgaattgaa agatctttct
     2401 tataaaggaa cattagacag aaatgatggt caaaccacga tcaactggca acccaacttg
     2461 gtttttccag aaatggaagg aaatgacaaa gtaagcctga ccacacaaga agcagcaaga
     2521 gggaatatta tagatcgaaa tggtgaacca ttagcaacaa caggcaaact aaaacaatta
     2581 ggagtcgttc caagtaaact tggagatgga ggcgaaaaaa cagccaatat caaagcgatt
     2641 gcttcctcct tcgacttaac agaagatgct atcaatcagg cgatttcaca aagctgggta
     2701 caacccgatt actttgtccc attgaaaatc attgatggag caacgccaga acttccagct
     2761 ggagctacca ttcaagaagt agacggcaga tattatcctt tgggtgaagc agctgctcag
     2821 ctgattggtt acgtgggaga tatcacagca gaagatattg ataagaatcc agaattaagc
     2881 agtaatggaa aaatcggtcg ctcaggtttg gagatggctt ttgataagga tcttcgtggg
     2941 acaacaggtg ggaaattaag cattacagat gcagacggtg tagagaaaaa agttctgatc
     3001 gaacatgaag ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa
     3061 acagcttttg acagtctagg aggaaaagct ggatcaactg ttgcgacaac gccaaaaacc
     3121 ggtgatcttc ttgcgcttgc tagctctcca agctatgatc caaacaaaat gacaaacggg
     3181 atctcacaag aagattacaa agcttatgaa gaaaatcctg aacatccatt catcagccga
     3241 tttgcgacag gttatgctcc tggatctacg tttaaaatga ttacagcagc aatcggtctc
     3301 gacaacggca ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa
     3361 gacagttctt ggggatctta tcaagcaacg cgtgtcagta gtgatgtatc acaagtagac
     3421 ttaaaaactg ctttgatcta ttccgataat atatatgcgg cacaagaaac gttgaaaatg
     3481 ggtgagaaaa aatttcgtac aggcttagat aaattcattt ttggtgaaga ccttgatttg
     3541 ccaattagta tgaatccagc acaaatttct aatgaagata gctttaactc agatatcttg
     3601 ctagctgata ctggatatgg acagggcgaa cttctaatta ctcctatcca gcaagcagca
     3661 atgtattctg tttttaccaa caatggcaca cttgtctatc ctaaattgat tgcagataaa
     3721 gagacaaaag ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat
     3781 ctgagagaag ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt
     3841 ccgttggcag cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa
     3901 gagaacagtt tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg
     3961 ttggaaaata aagaagatga tgattcagca accaaacgag catcggaact attgcaatac
     4021 ctcaaccaaa attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt
     4081 atcgtcacag tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt
     4141 ttgatttact cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat
     4201 aagtgtactt aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg
     4261 actttgctgt ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc
     4321 accgaccatc aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc
     4381 aagcaatttg gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact
     4441 aaagtcagaa aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc
     4501 atatcctaaa gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc
     4561 tatcccagca aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga
     4621 cattcctaaa caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc
     4681 tgcaaatgaa agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata
     4741 aattagctgt tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag
     4801 aaaagatagg ctgaaactta gaactaaaac gactaaaatg tcatctacca cagaagcacc
     4861 caaaattgtt gatccttctt ttgtattcac tacgtttagt tcttttaata cttctactga
     4921 aatactgaca gaagttgccg caagaataat cccaaagaaa atcgcttcat ttcccgaaac
     4981 ttggaatgct ttgcctgtca accagccaaa aacaaccggg aagatgatcc caagcaaagc
     5041 aacgaacata ccaggtcgga aatatttttt caaaagagat aaatcacttt ccagaccagc
     5101 taaaaacatt aataagatca caccaatctc cgaaaagtca tggactagaa tatcaggttt
     5161 gacccaattc aatccagctt ggcctagcaa aacaccaaca agtaattgtc caatcactgc
     5221 tgggatacct aatctccgcg agatatgtga gccaatcgtt gttgaaatca atatcaggca
     5281 taaaatacca ataaattcca ttctcatcat tcctttgtat aaaatatagc ccgaacttag
     5341 cataacatac attattgaaa aaataggaaa atatactcac aaaaaaacca acaatccaaa
     5401 agattttttt ggattgttgg tttttatacg gtctatttta ataactaaaa atgattaacg
     5461 tatagtcatt tttcttattc tgatgaaatt gataatctcc tggatgccca tgaaaatcaa
     5521 aataaagccg aatatttgga atagaagcat caaacttttg aatggattga acaataagac
     5581 gaatccgcca atcaacaaca gaatactact ataaacactc caacttttag cttttgtatt
     5641 catgctgaag aacaactgga acaagctgtt gatgataaca gctaatccaa gaagaatcgg
     5701 caggatagaa acgattgcag gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa
     5761 aagaccactg aacagaccga tactccaaga acctgttttc cttttaaaac ggtaagcctc
     5821 aaaaagattc aacaacccaa gcaaaacaag ataggcggtg atcagataac cgataaaatg
     5881 aaaaacagca gaagggttta gcacaatcgc aatcccagaa ataatataaa cagcagctct
     5941 taataaggcg tatttttgaa tattttgttt aatcgtctca aacatataag aaaacctcct
     6001 ttgacttatt ttaacgtgaa ctagtcattt taccaaatta aatgttacaa aatgagcgaa
     6061 ggagaagaat atctctggaa aaatcaactt ctgaggatct tttccatttt tttgcctttt
     6121 gctagctcat cgatcatttt atctaaataa cgaatttctt tcatcaagtt atcctcgatg
     6181 tcttctactc gtacaccaca aacaacacct gtaatcaagg aacgtgaagg attggggaca
     6241 ggggcttccg caaaaaattg ttcgaaagtc acttgttttt gtagttgata ttctagttct
     6301 gtttgggaat aacccgtcag ccaacaaatg atttcatcta cttcctcttt tatacgatct
     6361 tttctctccg cttttgctac atacatagga taaacgcttg caaaacttgt cgtaaaaata
     6421 cgattttgcg ccataatcag tcacctttct ttttttcaaa ttatttacta cttatagtat
     6481 ataagaaaaa gagaagaaaa agcgattatt caaaaaaaca aaaaggattg tgacaaaatt
     6541 cttgtcacca tccttttttc cgctctctga ctttttacaa tcttcatagc tcaagtgcgc
     6601 atcagataat cgaatgctcc taaagctgca gtagctcctg aacccattga aacaatgatt
     6661 tgtttgtatg gactatctgt acaatcacca gctgcgaaaa cgccggggat attcgttgcg
     6721 ccatttttct cggtcactat ttcatttctt ggattcattt cgatcgttcc atctagccaa
     6781 tccgtattag ggagtaagcc gactagaatg aatactcctg aaacagctag tgtatgcatc
     6841 tccatcgtac ggcgatcgat ataggagaga gattctacct gattcgtacc gctgatttct
     6901 ttcgtcgctg catgtttgat aacagtgaca ttcgcaagag agactagttt atcctgtaag
     6961 atttgatctg cttttaattc tggtaagaat tctagaacat atacatgttt tgctaaccca
     7021 gccaaatcga ttgctgcttc aacaccagaa ttaccaccac cgataacagc aatctctttt
     7081 cctttaaata aagggccgtc acagtgtggg caataagcaa ttcctttatt tttaaactct
     7141 ttttcgcctg gtacattgat agaacgccag tgcgcacctg tcgcgatgat ggcggttttt
     7201 gctttaagtg atataccgtt agctaagcca atattaataa aatgttgctc tttatttaag
     7261 gaaacggcct gttgattcat cataatgtcg atagggtaac tacgcacatg actttctact
     7321 tgctgcatca attttggacc ttctgtgtaa gaggtaccaa tcatattttc aatccctagt
     7381 gtttcaacga cttgtccgcc gaaagaatca gcgacaagtc ctgtacggat tcctttacgt
     7441 gctgcataga tagctgcact actagctgct ggtccaccgc caatcactaa tacatcataa
     7501 ggttcttttt caaacaactc ttcttcaatc aaagggccag tgattttttc aacaatttgt
     7561 tcgatcgtca tgcgtccgct actaaattct tctccattta aaaacacagt tggcactgcc
     7621 atgatttttt tatcatcgat ttctttttgg aacatcccac cttcaatcat cgtatgagta
     7681 atatttggat ttaatacagc taatatattc aatgcctgta caacgtcggg gcaattatga
     7741 caagtcaagc taacataact gtcaaaatgc aatgtttgtg ttattttttt gattttaaga
     7801 ctcaaagatt cactgatttt cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa
     7861 gaggaaaatt catgaccgag ggggatacca gcaaaaacga ttccactttc tttgccaact
     7921 ggattgatag aaaaactagg tgtgcgcatc gaatctcttt tttcccaaga gattttagga
     7981 gacatttctg ctatttcctt cgtaaattca gctagttttt gactattttc actattatcc
     8041 aaactaacag aaaaaacaat aggagattca atcagtgcta aatattgttc gagattttgt
     8101 ttagtttctt tgtctaacat atccaccacc taaatctttc caacaagatc aaaacttggt
     8161 ttcaatgttt cgccactttc tttccatttt gccggacaaa cttcacctgg gtgggtacgt
     8221 acatattgag cagcgcgaat cttatcaatc agtgcactag cgtcacgacc gattccatct
     8281 gcattgattt ccatagcttg aacgattccg tctggatcaa taataaatgt tcctcgctga
     8341 gctaatcctt gctcttcatc caaaacatca aaaatacgag aaatctgatg agacggatca
     8401 ccgatcatca cgtattcgat tttaccgata gcatctgatg tatcatgcca tgctttgtga
     8461 gtaaagtggg tatcggtaga gcaagaaaag acttcaacac ctaaagattt caatgtactg
     8521 tattgttctt gtaagtcttc taattcagta ggacaaacaa aagtaaaatc agctggatag
     8581 aagcaaagaa tactccagtt ccccataaga tctttttcac tgacttctaa aaattctcct
     8641 tgatgataag catgtgcatt aaattcttcg atttttttcc caatcaatga catagtaaaa
     8701 cctccaataa taattattta tttataatta ttgtaattta gttggagttg caaagtcaat
     8761 ggatattttg ttatttaata aagattactc aaaaacgaag gttaattata aaaatagaag
     8821 aggtcgcaag aaaatgctac tgctggaaat actcgggata acgttgagta acagtttcct
     8881 gttctttcag catcatgtgt aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt
     8941 tatcaactaa agccttgagt aaatcataat gttgttccat gatcgtttcc catggaagcc
     9001 cctgaatggt cactcgtaac cagcggaaac gttctaaatg ggtattatgg tcatctaacc
     9061 agttccaaac ttcttttctt cctgcaattt cgaaacaagc ttcatgaaat aaattatctg
     9121 cacggaaaaa agcgcgttcg tcatttattt tgactgcttt ttcttgttct tctaaaatcg
     9181 tttctagcac tattcgtgtt tgtgtgtcca atttggaaca acattcaatc atgatttgct
     9241 cttccaactg ttctcggaca aagcgagcat tttctgcaga aacaagatta attttggaaa
     9301 catacgtacc actttgaggt atcgtataca cgagttcttg ctgacgtagt tgaatcaatg
     9361 cttcgcgaat gggagtccgg ccgatagcaa gatgttcctc cagtgccttt tcagatattt
     9421 tccttccagg ttctaagtca gcatagataa tttgtttacg aatcgcttga taagcttgtg
     9481 attgcaagct gcttttcatc tttattcctc caaaatcatt tctatgaacg tagtataaca
     9541 gaagaggtct gaaaaacaat ctggtatgtc acattaacta gtaaatttgt ataaatagta
     9601 aaattttggt tgacatagcg atttttattg cataaaattc acttgaatac aaaaactaat
     9661 atgttagttt aggaggggaa aaatgattga tttaaacaac tggaggaact ttatccctga
     9721 attatctcaa aaagaagtag aaatacctca ttttgaccgt gcatcattaa gagaaaatgg
     9781 acaaaaaaat cctgtctgga tccatttcgg tggcggaaat ctttatcgag gatttcatgc
     9841 agaaatcgct caacgtttag cggatctagg cgaattgtcc actggtgtca ttgtttgtga
     9901 aacattcgat gaagaaataa ttgaaagggc ttaccaagat tttgatgatt tgattcttga
     9961 agtggtcatg ccaagcagtg gagagttcgt caaacgtata ttagctgcga cagctgacag
    10021 tctgtattgt catccagatt gttctgaaca ctttgataag atgattcgac actttgaaaa
    10081 tcctagttta caattagtta cttttgctat tactgaaaaa ggttatcgat tgaaagatac
    10141 aaataataaa tatataggac cgattgcagt cgatataaaa aatggtccaa agaagccatc
    10201 acatacaatg agtattcttg ctgtgctact ttataaacgt tttttatctg gagcttggcc
    10261 gattgcactt gtctctacag ataatttttc acaaaacggt agtcaattac gtacttcttt
    10321 gctaacgatc gcaaaagaat ggcaaaagga aggttatgta caaaatacat ttatccaata
    10381 cttatcgaat gaagaaattg ttgcgtttcc atggacgatg attgatcgta tcaccccgaa
    10441 tccttccttg tcagttagca aaatgttggc aaaagagggc ttttcaaata tgtcgatttt
    10501 ccaaaccgca aaaggaaata atattgctcc atttgtcaat acagaggaca tccattattt
    10561 aatcatggaa gatcattttc ccaatggacg tccgaaattc gaaaaagctg gggtattctt
    10621 gactgatcgt gcaacggtag ataaagcaga tacgatgaag gtgacgactt gccttaatcc
    10681 tttgcatacg tctttagcta ttatgggttg tttactgggg tataggagca tagctgagga
    10741 gatgaaagat ccagatttat cgctgctagt ttctgaaatc gggtacaatg aaggcctccc
    10801 agttgtggag tctccaggta tcattgatcc aaagatcttt ctggaagaag taattactgt
    10861 gcgcttttct aatccaaata ttcctaatat gcctcagcga atcgccacgg atacctctca
    10921 aaaggtggcg attcgttatg gtgagacgat taaaaaatat atggaaatag aagttaaaga
    10981 cccgcagaca cttgtattca taccattgac tctcgctgct tggcttcgat accttattgg
    11041 cattgatgat caaggaaaac cattcatccc gagtccagac ccactattga cagaccttca
    11101 gcataagctt tccgatattc aactgggtac gcaagataca tcacatattc atgagaaaat
    11161 ggctcctatt ttgtcaaata aagaaatttt tggtgtagat ctttaccaag cagagcttgg
    11221 cagaaaaata gaagaaatgt tcatcaagat ggtagcagga ccaaatgccg tacgaaaaac
    11281 attacaggat tacttacaag aacatggagg aaatttatca tggaaatgac ttttagatgg
    11341 tacggatcac aggaagaaaa gattacctta gatgaaatca gacagattcc tgcggtcaaa
    11401 gggattgtag gcacattgat ggatatacca gtaggagagg tatggcctag ggagaggatc
    11461 aaagaactaa aagaagaaat tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc
    11521 aatgtgcatg atgccattaa aattggatta ccttcccgtg agcagtatat cgaaaactat
    11581 aagcagacaa tccgaaactt ggcagaatat ggaatagaag tcatctgcta taattttatg
    11641 cctgtatttg actgggtgaa atctgacctt gactaccgct tagaagacgg ctcatccacg
    11701 ttagctttta tatctgatga tattccagat gatccaagag aaatcgtcgc gcgcatcgaa
    11761 cattccagca atgaatttgg attgccggga tgggaacctg agcgcttagc tcctatcaaa
    11821 tccttgcttg aagcctatgc atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat
    11881 tttctccaat caatcatccc aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca
    11941 gatgatccgc catactcgat ttttggatta gctcgaatcg taaagaatcg agaagattta
    12001 gattggttgt gtcgcatagt agacagccct tataacggaa tcactctttg tacagggagt
    12061 attgcagaag atccagaaaa tgatgtatat gggattttag cagaattttg ccaaagagat
    12121 cgaatcccat ttgcacatgt tcgaaatatt aaatttttag ctaatcgaga tttctatgag
    12181 gcaccacata aatccgaata cggttcatta gacatgtacc gcatactaaa aacaatgtat
    12241 gacaacggat ttaacggata catccgtcct gatcatggaa gaatgatctg gaatgagaca
    12301 ggaagaccag gatacggctt atatgatcga gcattaggtg ctgtttatct aaatggatta
    12361 tgggaagcaa tcgaaaaagg aaacaaaagt aaagataatt agtgaaaaaa actgtaattt
    12421 tattatgtaa ataagaagga tgttcaaatt cttgatacat aaatccggag gttgttaata
    12481 agcattcgga tttatgtatc ttatttggtc taggaagtaa cttgattttc ttttttcgat
    12541 tgatccatga gtgcttcttc ttttaattca aagatatatt ctgagatcct cataatcact
    12601 tttttccagt cttctggtgt gatgattcct tttaaaatat agattttttt gatatgagaa
    12661 ggcaattgtt tcaaagggca gtcgctgata acgatgttgc agttctccat aaaatcattt
    12721 tgtattttga tcgattcatt tgaaaaaatt tgttctatct taacgatcag atctttactg
    12781 atataaaaat cacgcgtata gttcacacca atcacaattc gcggctgttt tttgtagcga
    12841 tcatacaaat gtactaggtc ttcaaccatc cattgcacat tttccttttc taaaataact
    12901 ggtaacaagt gttgattttg ttctctgaaa tccatataaa attgttcaat ggcggtttgg
    12961 acttctttca atacgctatg ttcttgataa agcatgaaag taggaagggg cgtatttggt
    13021 tgattgaagt taagaaatcc attataaagt ttatttcgtg cgaatgccaa tgagtaaaga
    13081 atcttttctt tttcaggtat aaacaaatca aatacagtac aaagggactc caccatttct
    13141 cttgaataaa gaacatgagg aacgtttgct ttttttaaaa gtttgtattg ctccaagctt
    13201 gtttcttccg attcgatttt cgtgatagaa agtcgagcta aaatattcac taagatgcgt
    13261 tgttcgcgac aaattggcag attaggtcgc aaaatatcta cagagagatc aataaaaaag
    13321 tcaatatatt catactctgt ga
//
LOCUS       DACENJ010000061        13339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.061, whole genome shotgun sequence.
ACCESSION   DACENJ010000061 DACENJ010000000
VERSION     DACENJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.061"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            368..1165
                     /locus_tag="IW748_001279"
     CDS             368..1165
                     /locus_tag="IW748_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1104198.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            1143..1556
                     /locus_tag="IW748_001280"
     CDS             1143..1556
                     /locus_tag="IW748_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104199.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            1540..4185
                     /gene="mutS"
                     /locus_tag="IW748_001281"
     CDS             1540..4185
                     /gene="mutS"
                     /locus_tag="IW748_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1104200.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            4203..6311
                     /gene="mutL"
                     /locus_tag="IW748_001282"
     CDS             4203..6311
                     /gene="mutL"
                     /locus_tag="IW748_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1104201.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            6333..6893
                     /locus_tag="IW748_001283"
     CDS             6333..6893
                     /locus_tag="IW748_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1104202.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(6978..8966)
                     /gene="treP"
                     /locus_tag="IW748_001284"
     CDS             complement(6978..8966)
                     /gene="treP"
                     /locus_tag="IW748_001284"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1104203.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            9189..9902
                     /gene="treR"
                     /locus_tag="IW748_001285"
     CDS             9189..9902
                     /gene="treR"
                     /locus_tag="IW748_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1104204.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            9979..10425
                     /gene="msrB"
                     /locus_tag="IW748_001286"
     CDS             9979..10425
                     /gene="msrB"
                     /locus_tag="IW748_001286"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1104205.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            10595..11197
                     /gene="ruvA"
                     /locus_tag="IW748_001287"
     CDS             10595..11197
                     /gene="ruvA"
                     /locus_tag="IW748_001287"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1104206.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            11210..12211
                     /gene="ruvB"
                     /locus_tag="IW748_001288"
     CDS             11210..12211
                     /gene="ruvB"
                     /locus_tag="IW748_001288"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1104207.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            12240..12818
                     /locus_tag="IW748_001289"
     CDS             12240..12818
                     /locus_tag="IW748_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1104208.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            12870..>13339
                     /locus_tag="IW748_001290"
     CDS             12870..>13339
                     /locus_tag="IW748_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1104209.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKIT"
BASE COUNT         4492 a         2441 c         2914 g         3492 t
ORIGIN      
        1 ccagccaaac aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg
       61 caagctttcg aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat
      121 aagaagggat ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa
      181 gaagtagatg aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt
      241 cccatttttt tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt
      301 gaccttttct ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga
      361 atttagtgtg cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat
      421 tactgaatat cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg
      481 tgagaatgcc gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga
      541 cggcgtagat gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt
      601 tgtaaatgaa gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca
      661 agggatggtc tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg
      721 ttcttttatg gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc
      781 cagaaaacgt acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca
      841 agcaatgggc tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt
      901 tcagacaaat gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat
      961 gacaggtccc tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct
     1021 gactgcatta ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg
     1081 tatcttggaa ttggatgata cgacaggtca tgcaaaagaa atccagctga ttcagatcaa
     1141 cgatgatcga ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa
     1201 ttcaaaaaga agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct
     1261 acaaagaact tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa
     1321 tcaaacaagc gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaaa
     1381 aagaagcaat caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg
     1441 tagcttatag aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga
     1501 tacaagaaga aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat
     1561 actcctatga tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc
     1621 tatcgtttag gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt
     1681 ttggaattaa cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt
     1741 gtacctcacc atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta
     1801 gcgatttgcg agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg
     1861 atccagctag tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac
     1921 aattttttaa cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta
     1981 agtacgggag aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc
     2041 tctgcattgc agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa
     2101 cagctaagcg ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg
     2161 gaatttagct ttttgacaag cgaactagtc gatccgctgg aaaaagaagc aaccggcaaa
     2221 ctattgactt atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta
     2281 gaataccaac cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta
     2341 agccaatcta ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca
     2401 aaaacagcaa tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca
     2461 aaacaaatta aagcaagaca agaaatggtc gcttctttgc tagacagcta ttttgaacgt
     2521 atcgatttgc agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta
     2581 gctttcggta atgtcaatgg gcgagacttg atccagctga aaacttcttt agaacaagtg
     2641 ccaatgatca gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca
     2701 gaaatgcagc cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg
     2761 ccgttacaaa taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg
     2821 taccgttctg ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt
     2881 caagaaacag gaatcaagaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata
     2941 gaaattacaa aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact
     3001 ttagcaaatg cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc
     3061 gaagcagaag aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa
     3121 gtcaaaaagg ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg
     3181 ctacaaagtt ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt
     3241 gacaagcatc agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga
     3301 catcaggaat atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt
     3361 acaggaccga atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg
     3421 atggcgcaaa tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg
     3481 atttttaccc gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta
     3541 gagatgatgg aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt
     3601 gacgaactag ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc
     3661 gaatatatcc atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg
     3721 acggtacttg agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa
     3781 gacggagaag ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc
     3841 atacatgtgg caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc
     3901 ctgcatacgc tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag
     3961 aaaactaaaa cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac
     4021 catatacaag accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc
     4081 gaaaatgaaa cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca
     4141 atggatgcac taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat
     4201 cgatggccaa aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag
     4261 tagtggagcg accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca
     4321 gtactcagat tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata
     4381 acggagaagg cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta
     4441 agatccataa tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt
     4501 tgccaagtat tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac
     4561 aaggcaccta tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc
     4621 gtcaaggaac gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat
     4681 acgtgaaaac actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat
     4741 tgagtcatcc atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg
     4801 ctggaaatgg cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa
     4861 aaatgctgaa aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc
     4921 ctgaggtgac tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca
     4981 agaacttttc cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc
     5041 gtttccctat tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc
     5101 atccgacgaa gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta
     5161 acgccattca aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac
     5221 ggtttaagaa aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct
     5281 acgcttttga agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag
     5341 gtaccttttt cgtagaagaa ctaaagaaca catttgttga cagaattgtt gataagtctg
     5401 tggaaaatgt tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa
     5461 cagcgaaaac ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg
     5521 aaaaatctga aaagaaagaa tttcccattg tggataacat ggagaaaaca aatgaagaac
     5581 aagcagataa atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc
     5641 ctgaatttga tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag
     5701 aaacggaaaa accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa
     5761 cgtatctttt tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag
     5821 agcgcattaa gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag
     5881 aattgcttgt tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc
     5941 aaaaacactt gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta
     6001 ttgtacgggc ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga
     6061 tgatcgatat gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag
     6121 caatcatgat gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag
     6181 cacgtgtgct gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc
     6241 gtccagtatt gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag
     6301 acccgcatta agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac
     6361 caagaagaaa agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata
     6421 tcgatgaaac gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag
     6481 aaaaagcacg aactgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg
     6541 acacgactgt tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg
     6601 aattcatgct gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa
     6661 aaacaaagga acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga
     6721 cagacgaaga gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat
     6781 acggcatcca aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta
     6841 tcgtaggatt tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa
     6901 agcggactat ctgattttcg attgaaaatc catgatagcc cgtttttttt attgaaaaga
     6961 ttacttatct actctgacta agattttact tcagctttac cgactgcttc gcccgggcga
     7021 acaagtcctg attggtcaag agaaagtcct gcaacttttt cattattggt caaagcaaca
     7081 ataatgtcag ttttcttgcc agcttgttct atttctggca aatccatttc agcaatcagg
     7141 gtctctggtg tgacaaggta tccttctttc acgaatacat tgaaaggaac accctttagt
     7201 tcaacagtat ctaatcccat gtgaacaagt acttccagcc cctcattaga aagaatacca
     7261 atggcatgtt ttgtttcaaa gacactcgtc actttaccgt ttactggtgc atagactttg
     7321 ccgtttgacg gttcgacagc ataaccgtca cccatcattt tttgtgagaa aactgggtca
     7381 tttacttcag ttatttcctt gatggtccct gcagcgactg cgaataatgt ttcttttgtt
     7441 gcaactgccg taccagttgc tgaaacaggt tcgaaagact gtggcgttgc tgttgcgcca
     7501 tttgctgttt gaagttgtac atcagcagca ttttcaggaa cagcaggatc aatcttattc
     7561 aaaattccct gacgtctaaa gatgatcgtc aagccaaatg gaatgatgac agcaatgatc
     7621 attgcgatga tgaaaggtac ccatgtttct gcttggatag ctagtattcc tggcaaaccg
     7681 cctactccga tcgcgttagc tcgaacaccc atcaagttag caaacatgcc agctaatcca
     7741 gagccgacca ttgcagcgac gaaaggatag acgtatttca aattgattcc gaacatagca
     7801 ggttcagtta cgcctaagta acaagaaatc attgctggaa tcgagatttg ttcttctttt
     7861 ttattcccac gatgtaagaa gatgatggct aagactgcag agccttgggc gatatttgat
     7921 aaagcaatca tcggccataa attcgttgat ccgaaatctg caatcagctg catatcgatg
     7981 gcattggtca tatggtggag accagtaatc actaaaggag catacaggaa tccgaatacg
     8041 gcactaaaca accagctgat agaagacgtc aatccagtat ttacgatatt tgagatccag
     8101 cttccgactg tccagccgat aggacctaaa ataacgtgtg cagctaaaac agttggtaac
     8161 aatgaaaaca atggaacaaa aatcatcgag atcgactgag ggatatattt gcggaagaag
     8221 atttctagat acgctaacat aaaccctgcc agcatggctg gaataacttg cgcttgataa
     8281 ccaatcatct ggacttgagc aaaaccaaaa tcccaaaatg gaatatctgc tgcggctgtg
     8341 gaagctacac tataagcatt taataattgc ggagaaacta acgtaattcc taaaacgatt
     8401 cctaatattt gtgtagtacc catctttttt gcaatactcc aagtgatacc tactggaaga
     8461 aaatggaaaa ttgcttcacc cggtaaccac aaaaaggcat tgacaccatt ccaaaacttc
     8521 gagtgttcaa cgattgttcc accaagactt tcaaactgga taccttcaag tacatttctg
     8581 aatccaagga tcaatccacc gacaatgatt gcaggaatca gtggtgtaaa gatttctgct
     8641 aaaacggcaa tagcacgttg aacgggatga aggttttgtt ttgcagctgc ttttccttgt
     8701 tctttggaaa ccccttcaac tccagatact gcaacgaagt cgttataaaa agtagaaaca
     8761 tcgtttccta tgatgacttg gaactgtcct gcattcgtga acatcccttt tacgctaggt
     8821 atatcttcga tcgctttttc gtccgctttt cctggatcat tcaggacaaa acgcatacga
     8881 gtcgcacaat gactaactgc agcaatattt tcttttccgc cgatatcatg taacagcttc
     8941 tcggcatctg cttgatattt tgccatatca ataaacccct ttctcttaac ttgtctatgc
     9001 aagttgtttc ttcatttact atactttttt tcggagagaa ttgcaagcgt tttattaaaa
     9061 agtacgagag aaaatgatat cgttttaaaa agcttaaaat aagcttttct taaaaatagg
     9121 aacattgaaa tacaaaaaga gctgtagtat agtgaagaaa tgatggaaga acgagtgagg
     9181 gaaaaaacat gaataagttc aatgaaattt ttttagattt ggaacagaaa attttagcga
     9241 aagaatatcc tccgcattcg cttttaccca gtgagaacca attgatcaaa atttatggtg
     9301 tttcacgaga aacaattaga aaagcgctga atcttttgac tacggaaggt tatattcaaa
     9361 aaaaacaagg aaaaggttcg atcgtactgg atcggaatcg cttcgacttt cctatttctg
     9421 gattgactag ctataaagag ctacaggaga cccaacgtat tccaagtgaa acgatcatcc
     9481 atacgttaga agaaacagaa gtgacaaaag cattgaacga gatcactggc tgggagatcg
     9541 gtgcgcctgt ttggcatttg atacgcgaaa gaaaaatcaa tggcgaagta gttatattag
     9601 acaccgatta tctattaaag gaaatcgttc ctattttaac tccagcacaa gcacaaggat
     9661 cgatctatga atattttgaa aatgagcttt ctttgacgat tgcttacgcg caaaaggaga
     9721 tcacagtaga agaagtaacg gaaatcatga aaacagcgat ggatcttcac gatgacactt
     9781 atgcagtagt agtcagaagc ctcgttcatc tggaagatac ccgctgtttc gagtattctg
     9841 agtcggttca ccgattagat aaattccgtt tcgttgagtt tgcccgacgc cgaaaagtgt
     9901 aaatagttca aaagaaagca aattgttggc atctgtccac tttggcgcta tgataggatc
     9961 acaaaggggg catttttaat ggataaacca ataagaccag aaaaagaaga actgaaagaa
    10021 aagttatcac ccatggctta tgcagtcaca caagaaaatg caacagaacg accatttagc
    10081 gggaaatatg atgattttta cgaaaagggg atctatgtag atatcgtcag cggtgaaccg
    10141 ttattcagct ctgcagaaaa atatgatgca ggttgtggct ggccatcatt cagcaaacca
    10201 ataacgaaaa gacaagtgcg cgaaaaagcc gatttttctc atggcatgca tcgaatagaa
    10261 gttcgaagca aagaagcaga ttctcatctc ggacatgtgt ttaccgatgg accagtagat
    10321 caaggtggtc tacgttactg catcaattct gcctcactga aattcattcc atatgaaaag
    10381 atggatgaat taggttatgg ggaattcaaa tcacttgtag aataatggaa atacacaaag
    10441 agcggtacaa tacggggatt tactcgatcg tccgctcttt ttttattcaa gcgattgcta
    10501 tttgaaagaa acgctcccta gactcctttt ttcacaaggt gaaatgtgct acaataaagg
    10561 gaacgtatgt gtgcaaaaga aagaggacta gaatatgtat gaatatctga caggtgtggt
    10621 tacctttatc aacccctatt acatagtagt agaaaccaat ggtatcggct accagatcgc
    10681 actaggaaat ccttaccgct acagtagcaa actaaatcaa aaaataaaaa tttatgtcca
    10741 tcaggtaata cgtgaagatg cgcatcttct gtatggattt gattcgctag aagaaaagca
    10801 actgtttttg cgattagtaa gtgtttcagg aatcggtccg aaaagtgccc ttgcaatcat
    10861 ggcaagcgaa gaccactccg gtttgattca agcagtagaa tccagtgatg tcacttattt
    10921 gacgaaattt ccaggagtag ggaaaaagac agcacaacaa atgatcttgg atctaaaagg
    10981 aaaattcggt gagctgagta tcgatacacc ttttagtcta tttgatgaag caaacacaaa
    11041 agacgctaca gctctatctg aagcaatgga agctctctcg gctcttggtt acagtgatcg
    11101 agaaatcaaa cgtgtagaaa aacaattaaa agaagtagat aatcagacaa cggatgagta
    11161 tctacgccaa gcactcaagc taatgatgaa aaaataagga ggagaccaaa tgacagatga
    11221 atacttgtta tcaccagaat ctggcgaaaa tgaactgagt ttggaaaaat ctcttcgacc
    11281 acagctgctt tctcaatata tcggacagag aaaagtcaaa caagaacttc agatttacat
    11341 cgaagctgcc agaaatcggg aagaaccttt agatcatgtt ctgttgtacg ggcctcctgg
    11401 attaggaaaa acgacgatgg ccatggtcat tgctaatgag atgaccgtaa atattcgtac
    11461 gacgagtggc cctgctatcg aacgtccggg tgacttagta gcaatcttga atgaactgga
    11521 agcaggggat gtcctgttta tcgatgagat ccatcgatta cctagagtgg tagaagagat
    11581 gctttattca gctatggaag atttctatgt agatatcatg gtaggacaag ggccaaccgc
    11641 acatccggtt catttccctc ttcctccctt tactttgatt ggcgcgacga cacgggcagg
    11701 tatgctttct gcacctttac gagatcgatt tggcatcatt tctcatatgg aatattatga
    11761 cgaacaggat ctgcaggaga tcgttttacg ttcttcggat atttttcaaa cagagatcat
    11821 tgaagaaggg gcattggaaa ttgctcgccg atcacgagga acgcctagaa tcgctaatcg
    11881 cttgctcaaa cgggttcgtg attttgcgca agtccaagga gacgggaaaa tcgatcgtcc
    11941 tatagcggat caggcactta ctttgctaca ggtcgatcag gctggattgg attatgttga
    12001 ccagaaattg ttgcgaacaa tgatcgagtt atatggcggc ggaccagtag ggttaagtac
    12061 gctttcagta aacataggag aagagcgtga gacggtagaa gatatgtatg aaccgtatct
    12121 gatccaaaaa ggatttttaa agcgtacacc tagagggcga attgctactg cttatgccta
    12181 tgaacatttt ggttacgact atcatatatg atttttgctt ttagattgga ggaaaatgaa
    12241 tgagtgatac aacacgtacg aaacaattga tcaaaaacgt attgattgat ttgtgccaaa
    12301 cgcaatcttt tcgaaaaatc agcgtccaaa aaatcgcaaa cgaagcaaaa atcaatcgac
    12361 aaacatttta ttatcatttt acagataaat acgatttgct tcgttgggtc tattgtgagg
    12421 atgcattgca ttatttagat ccacaatgtc tttcacttga taattgggaa gagcaagcat
    12481 tgaaaatgct gaaagcaatt gctgagaaga aagaatttta tgcaaatact gtgctttctg
    12541 acccagaaat tttacaaaaa gagttttctc aactgatcca aaaatcattt atcaagattt
    12601 ttgaacaaat ggatgaagaa agacaacttt ctgcaagaga caaactattt tatgctcgtt
    12661 ttttttctta cggttgcagt ggtgtattag tcaattggat cacgaatgaa tatgaagaaa
    12721 caccgatgga aatcgctatg cagcttttcc gtttagccaa ggatacggaa ttatattctt
    12781 atcgtcttta tgcaaaagaa gagttggaag aacagtaata gaaaaatttt ttgttggaaa
    12841 agcaataatc gctaaaagga gatggggaaa tgacaaaaat tttatttgtt tgtttgggaa
    12901 atatttgccg ttcaccgatg gcagaaggct tgctgagaaa acgaatagca ttagaaggaa
    12961 gggaagcaga attttttgtg gattctgctg ctacaagcac gtatgaagtt ggaaagacac
    13021 cgcaccctgg aacgaaaaaa atattaaacc aagaaaaagt agatatgacg aatatggttg
    13081 ctcggcaaat cacaccccac gattttgaaa cttttgattg gatcattggc atggatcaag
    13141 aaaatgtaga agaactgaaa cgaagagcac cacgctcagc gcaggggaaa atccatttgt
    13201 ttttaagtag cattcctgga aaagaaaaag aaaatgttcc cgacccatac tataccaata
    13261 actttgatga gacatatcac ttgatcacag aaggcctgaa tcattggtgg aatatttgga
    13321 ttgatgaaaa taaaataac
//
LOCUS       DACENJ010000062        13330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.062, whole genome shotgun sequence.
ACCESSION   DACENJ010000062 DACENJ010000000
VERSION     DACENJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.062"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(353..2080)
                     /locus_tag="IW748_001291"
     CDS             complement(353..2080)
                     /locus_tag="IW748_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1104210.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2073..3266)
                     /locus_tag="IW748_001292"
     CDS             complement(2073..3266)
                     /locus_tag="IW748_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1104211.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3453..4097)
                     /locus_tag="IW748_001293"
     CDS             complement(3453..4097)
                     /locus_tag="IW748_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1104212.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4191..4325)
                     /locus_tag="IW748_001294"
     CDS             complement(4191..4325)
                     /locus_tag="IW748_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1104213.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4327..6459)
                     /gene="feoB"
                     /locus_tag="IW748_001295"
     CDS             complement(4327..6459)
                     /gene="feoB"
                     /locus_tag="IW748_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1104214.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFFMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6456..6929)
                     /locus_tag="IW748_001296"
     CDS             complement(6456..6929)
                     /locus_tag="IW748_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1104215.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7330..7554
                     /gene="nrdH"
                     /locus_tag="IW748_001297"
     CDS             7330..7554
                     /gene="nrdH"
                     /locus_tag="IW748_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1104216.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7713..9872
                     /gene="nrdE"
                     /locus_tag="IW748_001298"
     CDS             7713..9872
                     /gene="nrdE"
                     /locus_tag="IW748_001298"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1104217.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9922..10887
                     /gene="nrdF"
                     /locus_tag="IW748_001299"
     CDS             9922..10887
                     /gene="nrdF"
                     /locus_tag="IW748_001299"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1104218.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10976..12115)
                     /locus_tag="IW748_001300"
     CDS             complement(10976..12115)
                     /locus_tag="IW748_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1104219.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12424..13137)
                     /locus_tag="IW748_001301"
     CDS             complement(12424..13137)
                     /locus_tag="IW748_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1104220.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
BASE COUNT         4103 a         2762 c         2329 g         4136 t
ORIGIN      
        1 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
       61 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
      121 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
      181 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttgaacgt ttcgaagatg
      241 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
      301 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
      361 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
      421 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
      481 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
      541 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
      601 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
      661 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
      721 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
      781 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
      841 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
      901 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
      961 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
     1021 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
     1081 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
     1141 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
     1201 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
     1261 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
     1321 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
     1381 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
     1441 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
     1501 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
     1561 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
     1621 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
     1681 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
     1741 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
     1801 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
     1861 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
     1921 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
     1981 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
     2041 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
     2101 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
     2161 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
     2221 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
     2281 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
     2341 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
     2401 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
     2461 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
     2521 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
     2581 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
     2641 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
     2701 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
     2761 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
     2821 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
     2881 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
     2941 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
     3001 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
     3061 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
     3121 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
     3181 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
     3241 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
     3301 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     3361 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     3421 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     3481 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     3541 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     3601 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     3661 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     3721 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     3781 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     3841 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     3901 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     3961 gcattgtacg tactcgcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4021 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4081 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4141 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4201 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     4261 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     4321 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     4381 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     4441 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     4501 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     4561 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     4621 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     4681 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     4741 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     4801 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     4861 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     4921 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     4981 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5041 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5101 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5161 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     5221 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     5281 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga aaaaagcgat
     5341 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     5401 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     5461 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     5521 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     5581 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     5641 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     5701 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     5761 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     5821 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     5881 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     5941 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6001 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6061 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6121 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6181 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     6241 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     6301 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     6361 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     6421 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     6481 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     6541 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     6601 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     6661 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     6721 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     6781 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     6841 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     6901 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     6961 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7021 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7081 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7141 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7201 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     7261 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     7321 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     7381 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     7441 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     7501 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     7561 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     7621 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     7681 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     7741 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     7801 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     7861 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     7921 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     7981 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8041 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8101 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8161 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     8221 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     8281 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     8341 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     8401 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     8461 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     8521 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     8581 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     8641 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     8701 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     8761 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     8821 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     8881 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     8941 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9001 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9061 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9121 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9181 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
     9241 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
     9301 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
     9361 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
     9421 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
     9481 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
     9541 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
     9601 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
     9661 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
     9721 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
     9781 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
     9841 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
     9901 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
     9961 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10021 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10081 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10141 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10201 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    10261 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    10321 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    10381 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    10441 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    10501 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    10561 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    10621 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    10681 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    10741 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    10801 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    10861 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    10921 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    10981 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11041 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11101 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11161 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    11221 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    11281 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    11341 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    11401 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    11461 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    11521 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    11581 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    11641 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    11701 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    11761 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    11821 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    11881 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    11941 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
    12001 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12061 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12121 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12181 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    12241 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    12301 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    12361 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    12421 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    12481 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    12541 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    12601 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    12661 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    12721 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    12781 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    12841 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    12901 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    12961 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13021 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13081 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13141 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13201 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    13261 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    13321 tttcctttgt
//
LOCUS       DACENJ010000063        13314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.063, whole genome shotgun sequence.
ACCESSION   DACENJ010000063 DACENJ010000000
VERSION     DACENJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.063"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..92)
                     /locus_tag="IW748_001302"
     CDS             complement(<1..92)
                     /locus_tag="IW748_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1104221.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGV"
     gene            365..2836
                     /locus_tag="IW748_001303"
     CDS             365..2836
                     /locus_tag="IW748_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1104222.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2939..4675)
                     /locus_tag="IW748_001304"
     CDS             complement(2939..4675)
                     /locus_tag="IW748_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1104223.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4672..5376)
                     /locus_tag="IW748_001305"
     CDS             complement(4672..5376)
                     /locus_tag="IW748_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1104224.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5507..6010
                     /locus_tag="IW748_001306"
     CDS             5507..6010
                     /locus_tag="IW748_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1104225.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5970..6308
                     /locus_tag="IW748_001307"
     CDS             5970..6308
                     /locus_tag="IW748_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1104226.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6329..7747
                     /gene="ffh"
                     /locus_tag="IW748_001308"
     CDS             6329..7747
                     /gene="ffh"
                     /locus_tag="IW748_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1104227.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7859..8437
                     /locus_tag="IW748_001309"
     CDS             7859..8437
                     /locus_tag="IW748_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1104228.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8441..8962
                     /locus_tag="IW748_001310"
     CDS             8441..8962
                     /locus_tag="IW748_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1104229.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDVEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9041..9595)
                     /locus_tag="IW748_001311"
     CDS             complement(9041..9595)
                     /locus_tag="IW748_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1104230.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9848..10123
                     /gene="rpsP"
                     /locus_tag="IW748_001312"
     CDS             9848..10123
                     /gene="rpsP"
                     /locus_tag="IW748_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1104231.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10135..10386
                     /locus_tag="IW748_001313"
     CDS             10135..10386
                     /locus_tag="IW748_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1104232.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10511..11302
                     /locus_tag="IW748_001314"
     CDS             10511..11302
                     /locus_tag="IW748_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104233.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11472..11993
                     /gene="rimM"
                     /locus_tag="IW748_001315"
     CDS             11472..11993
                     /gene="rimM"
                     /locus_tag="IW748_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1104234.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11983..12744
                     /gene="trmD"
                     /locus_tag="IW748_001316"
     CDS             11983..12744
                     /gene="trmD"
                     /locus_tag="IW748_001316"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1104235.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12880..13227
                     /gene="rplS"
                     /locus_tag="IW748_001317"
     CDS             12880..13227
                     /gene="rplS"
                     /locus_tag="IW748_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1104236.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
BASE COUNT         4397 a         2436 c         2746 g         3735 t
ORIGIN      
        1 acgcctttat attctttttc aatcagccat tcttcatcaa aaaataaatg tttatctgtt
       61 aatccgagta attttctagt atgattatcc atagagcgac tcctttggta gggtgatgtt
      121 ttggtcgact aaattttacc aaaagtgagt ctctttttga gttgaaacga agaaaaagtg
      181 ttagtagaaa atcgccttcg attttccacc aacacaaaat attatagaac cctttttttt
      241 tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac tttccatatc
      301 tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca atcgaggtag
      361 ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac aaagatcatc
      421 agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag atcaagtcaa
      481 ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca aaacaaatca
      541 gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta tcttagccat
      601 tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta tcatagccaa
      661 tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg aactaaatgt
      721 tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa tagatataga
      781 agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc cagcagatgc
      841 agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg aagcagatga
      901 aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt ccggaaaagc
      961 ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga cagtcaaagc
     1021 caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc aattgatcaa
     1081 atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa gttacttttt
     1141 taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat
     1201 acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga ctaaactagc
     1261 aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa gggtaaatgt
     1321 cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc aaaaaatgat
     1381 cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat gcattgctga
     1441 ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag catattttac
     1501 aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc aaaaattcag
     1561 cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga
     1621 tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt
     1681 ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag cagtcacacc
     1741 attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca catttgatta
     1801 cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc tcaccgtatc
     1861 caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg catctaaatt
     1921 atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac gcgtcaagcc
     1981 agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag ctatgacagg
     2041 tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag cgatggcttc
     2101 cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg acttttcacg
     2161 catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc gatcagccag
     2221 cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag tgatctttgc
     2281 tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta ctatcggact
     2341 tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga aattcatcat
     2401 gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag tcggtgcgtt
     2461 ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag catctaccat
     2521 gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc tgaataaatt
     2581 cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca tctttttgca
     2641 taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga gcattctatt
     2701 tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt tgtttgaacg
     2761 taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc tatcgatatt
     2821 taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca accactcgtg
     2881 tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt gctctttttt
     2941 acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg tctacagtaa
     3001 tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat ccggtcccgc
     3061 ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc cgttcctgat
     3121 cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca tctgttgcct
     3181 gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt tcaatcagat
     3241 ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc tttccaatca
     3301 atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg atttgctgga
     3361 aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct tttgacagct
     3421 ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg atctcaagaa
     3481 aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg gtaaaaccaa
     3541 tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa tccttttgta
     3601 gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc tgtccggtac
     3661 cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct tctgtaaggg
     3721 tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc tgaatgagtt
     3781 ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc acgtttaaat
     3841 gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca atcaggaata
     3901 cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta gtacacgccc
     3961 gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat tcttcccatt
     4021 gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt ttttttagta
     4081 cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag atcattcctg
     4141 caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa ctctcagttc
     4201 tcggtaaaaa aacgcttgtc ggttctgcga tacgtaaaat ggcttctaac tggccacttt
     4261 ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt ttgcgcaaag
     4321 cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag tttcgctgtt
     4381 cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc attaaagtaa
     4441 tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc gctgcttgga
     4501 ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg tagctttctt
     4561 gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca ccaaaaaaca
     4621 aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat ttcatcgttt
     4681 gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg ggtgttttgg
     4741 atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc ggctttgtcc
     4801 tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca cacggtcttt
     4861 tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa tcagatggtc
     4921 gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg atagatattc
     4981 aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata ttgctttcat
     5041 acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag cacctatttc
     5101 taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa tcggtgtttc
     5161 atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg gaagcatcac
     5221 atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt cgcctccatc
     5281 tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga gcgttacaat
     5341 agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta aataggtttc
     5401 attctgttta tattttagca tagaatcagt acaaagggct attttacaaa agaaataaat
     5461 ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga ttggaatcag
     5521 tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag taccagaatt
     5581 gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga tcggcggatt
     5641 accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg tgtggaaaga
     5701 tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca aaaatggcgc
     5761 gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca tgaaggaaaa
     5821 aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa taaaaaaaaa
     5881 tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc tctctgatga
     5941 agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat cgaatgaacg
     6001 cgctctttga attttattcg accttattga cagaaaaaca aatgaactac atggaattat
     6061 attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta agccgacaag
     6121 cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa aaaaaactcc
     6181 atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact gcatatatta
     6241 cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt caagcaatcg
     6301 aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg aacgtctcca
     6361 gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg taaaagaaat
     6421 gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag tagtcaaaga
     6481 ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa gtttatcacc
     6541 tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg gttctgaaac
     6601 agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg gtcttcaagg
     6661 ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa atgaaaatgc
     6721 tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc aattgaaagt
     6781 attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca gcccggtaga
     6841 gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg tattgatcga
     6901 tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac aaatcaaaga
     6961 attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc aagatgccgt
     7021 caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca tcactaaact
     7081 tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag gcgcaccaat
     7141 caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc cagatcgtat
     7201 ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag cgcagcaaga
     7261 ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt cattcgattt
     7321 caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg aagatctaat
     7381 caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg tagatccaaa
     7441 agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa gagcaaatcc
     7501 tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa acagtgttgt
     7561 tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc agcaaatgtc
     7621 caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg tcaaaggaaa
     7681 actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa agaaaaaacg
     7741 gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc cggagctttt
     7801 ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg tggcacagat
     7861 gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata ctgtctgggg
     7921 tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata ttaatcaagc
     7981 cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag catatgatcg
     8041 attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac taatggaaaa
     8101 caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg ccaaagcagt
     8161 cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt tttctaatgg
     8221 aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa gtcctctttc
     8281 agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa gcacaacaat
     8341 ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt gtttccgcaa
     8401 agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa tcatatttta
     8461 tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc aatggctttt
     8521 tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa tcgatacgac
     8581 catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg aagcaattta
     8641 tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc cagatgttga
     8701 ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag aaggaaaaga
     8761 tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc aaatgattga
     8821 tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt tattccaaaa
     8881 aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg aatttatcga
     8941 attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat atgcagaaat
     9001 gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt aatttgttag
     9061 aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct tcaacgatat
     9121 taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt tgctgcaagg
     9181 ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca aagccgccga
     9241 tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca ctgtgtccat
     9301 aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa cggttgtatt
     9361 caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa cgagtccatt
     9421 cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga tcatcgattt
     9481 ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct gctacttttt
     9541 tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt gtcatacgat
     9601 ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg gagcaaataa
     9661 aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac aaaaaagaag
     9721 tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa gaaaaaaagc
     9781 tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat ggaggtggaa
     9841 ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga gtccgtttta
     9901 ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg aaactgtagg
     9961 tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact tagttcttga
    10021 ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt caaaagaagg
    10081 cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc ggatatgaaa
    10141 gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc tgatcaagtc
    10201 aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt ggctcctgaa
    10261 gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg cacaatcgtt
    10321 tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga tgataaggaa
    10381 aagtaaataa acgtgctgta ctcgaatatt cgagtgcagc ttttttgttt tttttattaa
    10441 ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag gaggagggag
    10501 tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt taagcgcttg
    10561 tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat ctgaaactgt
    10621 taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa caatggctac
    10681 ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc caaatgttcc
    10741 catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga cacatacaaa
    10801 gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga atgctgacga
    10861 attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg cttctagtca
    10921 agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc aagtggactt
    10981 aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc ttgaatggca
    11041 agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag atcccttact
    11101 attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac ctgaacaatt
    11161 tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag taagctggca
    11221 agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt tgaagatggc
    11281 agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat ttaactccaa
    11341 gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg cttaatacct
    11401 taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca aaaaagaaat
    11461 ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca caaggtatca
    11521 aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac aaaaaaggaa
    11581 cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc aaaagtcatc
    11641 gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc aatgatgtgg
    11701 aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat ctagctgaaa
    11761 atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga actgagttag
    11821 gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt caacgtccga
    11881 aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc gatttagaag
    11941 ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat tgatgtattg
    12001 actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg taaggctgta
    12061 gataaaggat tattagatat caatatctct aacttccgcg attattcaga taacaaacat
    12121 cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa agtccagccc
    12181 atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa aagagtaatt
    12241 cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt ttcacaagaa
    12301 gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat ccgcacatta
    12361 gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt aggtgcgatg
    12421 gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca gacttcagct
    12481 caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg cccggcagaa
    12541 tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt gattgaagaa
    12601 tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct tttggaaaca
    12661 cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga agaacaagaa
    12721 aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta ggttgataat
    12781 atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc cgctgtgaga
    12841 aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt aatcgaagaa
    12901 ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga cactgtgcgt
    12961 gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga aggtgttgtt
    13021 atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt ttctaacgga
    13081 gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat cgaagtagtt
    13141 cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca cggaaaagct
    13201 gctcgtatca aagaaatccg tcgttaatca ttgttatcag actgagaaag aacccttggt
    13261 attcaagggt tcttttttct tagcatgtaa tttgaacacc ctgatccagt tttt
//
LOCUS       DACENJ010000064        13296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.064, whole genome shotgun sequence.
ACCESSION   DACENJ010000064 DACENJ010000000
VERSION     DACENJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.064"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..291)
                     /locus_tag="IW748_001318"
     CDS             complement(<1..291)
                     /locus_tag="IW748_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1104237.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRA"
     gene            complement(468..540)
                     /locus_tag="IW748_001319"
     tRNA            complement(468..540)
                     /locus_tag="IW748_001319"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(505..507),aa:Lys,seq:ctt)
     gene            921..1490
                     /locus_tag="IW748_001320"
     CDS             921..1490
                     /locus_tag="IW748_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1104238.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1477..2337
                     /locus_tag="IW748_001321"
     CDS             1477..2337
                     /locus_tag="IW748_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1104239.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2527..3231
                     /locus_tag="IW748_001322"
     CDS             2527..3231
                     /locus_tag="IW748_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1104240.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3236..5071
                     /gene="walK"
                     /locus_tag="IW748_001323"
     CDS             3236..5071
                     /gene="walK"
                     /locus_tag="IW748_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1104241.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5068..6384
                     /locus_tag="IW748_001324"
     CDS             5068..6384
                     /locus_tag="IW748_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104242.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6385..7254
                     /locus_tag="IW748_001325"
     CDS             6385..7254
                     /locus_tag="IW748_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104243.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7309..8118
                     /locus_tag="IW748_001326"
     CDS             7309..8118
                     /locus_tag="IW748_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1104244.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8221..8850)
                     /locus_tag="IW748_001327"
     CDS             complement(8221..8850)
                     /locus_tag="IW748_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ1104245.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9020..10312
                     /locus_tag="IW748_001328"
     CDS             9020..10312
                     /locus_tag="IW748_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1104246.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10388..11050)
                     /locus_tag="IW748_001329"
     CDS             complement(10388..11050)
                     /locus_tag="IW748_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1104247.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11275..11559
                     /locus_tag="IW748_001330"
     CDS             11275..11559
                     /locus_tag="IW748_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1104248.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11610..13235
                     /gene="groL"
                     /locus_tag="IW748_001331"
     CDS             11610..13235
                     /gene="groL"
                     /locus_tag="IW748_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1104249.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4396 a         2304 c         2869 g         3727 t
ORIGIN      
        1 tgcacggcca ataatgacgt ctggggtcca accttcagtt gatctttttt caatgtattc
       61 tttttcctca gcaggaaaaa tgatttcggt tctcccacaa cgtcgcttat tttctttgta
      121 ttgttcaaag tattcaagtg ctgttccacc acatttgaga aagttataga cattgtaaat
      181 tgtttggcgt ccacgtttaa gctgcttcgc aacgatagca accggagttt cttgatgaaa
      241 atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca tttatgttca
      301 ctctccttgt atgctttagc gggtattaca atttgagtgt aacataaatg gcttttttat
      361 ttgtctcgct taattataca aacggcgaaa ataaaaaaag aacagcgtaa atgttttcga
      421 aatgctttcg caccaacatt tacctgttcc gtgggtttgg cacccaatgg gccgtgaggg
      481 gctcgaaccc gcgacccgct gattaagagt cagctgctct accaactgag ctaacggccc
      541 gtacaaaaag tacttaataa tagtagcact aagaatttta aaatgcaaga ctacatttaa
      601 agaaaatcag aaaaatcttt acttccttat aggaaatcat tcatagatta ttcacatttg
      661 ctcgctatta tataagcata ccaacaaaca aagttcttgc aagagaacaa accctaacac
      721 ttttttcatt ttttactcct ttcccgccgt ctccccggcg ggtctcctgt tcttaacgat
      781 aaaagacaag aaggcttagc cttcttgttt tttgttttca tggaatcccg ctaatcaaat
      841 cacctagtta aagcaactag attgaatgtt tatcataagt ctgatatcat gtttatgaaa
      901 tgaaaaaagg agcggttatg atggaaaaaa tgcgtcagca attaatggag tgggggaagg
      961 aactagaaac attccgtctg cctcactggg aagaactccc tgacttagaa ctttatatgg
     1021 accaagtcag aacgttagtt gatcgttatc tttcacctgt gatacaagga gaaaagcatc
     1081 cattgcttac ctcttctatg gtaaacaatt acgttaaact aggtttgata ccagcaccag
     1141 ttaaaaaaag atataacaaa gaacacgtag catttttact tgcaatcact acgttgaaac
     1201 aagtattgac tattcctgaa atcaaagaag ggatattgtt ccaaggaaaa acagtaggaa
     1261 ttcgtgaagc ctataatcta ttttgcgatg aacaagaagc agctgtgtgg atggtcagcc
     1321 aattagcaca aggtaaatct catccgcaaa aattcgacca ggcaacccct gtagaatata
     1381 ttgctgtaag agcggcaact ttatcgtttg caatgaaact tcttgcggaa aaaacgattg
     1441 ttctagaaac cgaatattta aaagaggaga aaacaaatga aaaacaataa aatcgcaatt
     1501 ttagtagatt caggtacgga tgtgccagaa gaattgataa aaaaatacga gatgtatatg
     1561 atcccattga aaattattta taaagatcag gtatatacag ataaaattga cattacctct
     1621 gaagagatct atcaacgttt gccagaggaa atccctggta catctttgcc ggatggtgag
     1681 tcgatcacag aaatctttga gcaaatcaaa aaagacggtt atgaaaaggt acttgcggta
     1741 acaatttcca gtggattgag cgggacatat aatgtggttc gtttaattgc acaacagcaa
     1801 gaagaattgg aagcatatgt actagatacg aaaaatattg gtattggtgc aggtttttct
     1861 gcgatccaag cagcaaaatg gctggaagaa ggagtagaat gggatcaact gatcagtaat
     1921 ctgaatgagt tagtcgaaag aacaaaagtt ttctttaatg ttgcgacatt ggaatatctt
     1981 caaaaaggtg gacggatcgg tttagttgct tctattgtag gaaccgcttt aaagcttaac
     2041 ccaatcatct catgtaatga agaagggatt tattacacag taggaaaagc taggggacgt
     2101 aaaaaaagtt tggataaaac ggttgcttac gtcaaagaaa aaatcggtaa tgcaaaggtc
     2161 ttcaacttag ctgttgccca tggggatgcg aaagaagaag caattgaaat ggaagcgaga
     2221 cttaaaagag agttcccaca agcaaaccaa atctactttg gccagatttc tccggcatta
     2281 gtagtccaca ctggtccggg attattgggt gtcggtgtac aagttttaga aggataaatc
     2341 aaaaaaagga aacactgagg tgaaaatcag tgttttcctt ttttattgaa gttagaacaa
     2401 aaaataataa agtattttgc actttcattt taattttctc ttttgtttta taggcaaaga
     2461 tgaaacaaaa gcagaaatac ggtataatga gaaggtatga caataatata taagaggtgg
     2521 ctctttatga aaaaagtttt agttgtcgat gatgagaagc cgatttcgga tatcgtcaaa
     2581 ttcaatttag ccaaagaagg ctatgatgtt tatactgcat atgatgggga agaagccttg
     2641 gaaaaagttg ctgaggtaga acctgatttg attttacttg atttgatgct acctaaaatg
     2701 gacggcttag aagttgctcg tgaggtacgc aaaacttatg atatgccgat cattatggta
     2761 acagccaaag attctgaaat tgataaagtc ctaggcttag agttaggcgc cgatgattac
     2821 gtgaccaagc cattttccaa ccgagaatta gttgcacgtg tcaaagcaaa tcttcgtcgc
     2881 ggtgccacgg ctgcgaagga accagaagag gcagcacctg cggaattgac gattggtgat
     2941 ttgacgatcc atccagaagc ttacatggtg acgaaacgag gagaaacaat cgagttaaca
     3001 catcgtgaat tcgaactact cttttacttg gcgaagcacc ttggacaagt gatgacaaga
     3061 gaacatttat tacaaacggt ttggggttat gattattttg gagatgtacg aacagtggat
     3121 gtaactgtac gtcgtttgcg tgaaaagatc gaagataatc caagccatcc aaattattta
     3181 gtgacacgtc gtggtgtcgg ttactatttg cgtaacccag aacaggagta attggatgaa
     3241 aagaaaagtc cgcttttttc gatcagtcaa ttttaagatc gctattacct ttattctgat
     3301 tttattgatc tcgatcgaaa ttattggtgc ctactttata cgtggtttag aacgttccac
     3361 gatcaataca tttattaaag atatgaatca aacggtggaa tcattagcaa caacaatcag
     3421 cccggaattg aatcgaaaag ataatgcaga tgatgaagag gtaaatgcga atatcaaaag
     3481 atttattgaa aacagtgcga ctagcgatat catcgaaata cgggtcgttg acgaaaaagg
     3541 gatcatccga ggaacaaccg atgtcaatga acaaagcgct gtgggaaaga aaaatgatta
     3601 tgttgatatc aatgatttca cgactaaacg ttatgttgct ttagataacg acgataaacg
     3661 ggtgaatatc aatgttcaac cgatcctgtc cccgaccggc gatgccgtta tcggggctct
     3721 ttacgtcaaa agtaatatcg aacaaaaata ttcggaaatc aataacacag cagtcatttt
     3781 ctttactgcc tcactaattg cggcatttat ttcaatgatc gtatctgttt tagttgctcg
     3841 ttcgattaca cagccgatcg gtgaaatgag agaacaagcc attcgaatcg ctagaggaga
     3901 ctacagtcgg aaagtcaaag tgcatggaca agatgaactt ggacagctgg ctgatacctt
     3961 caatcagtta gctgaacgta ttgaagaagc tcaagataca atggaagctg aacgcaatcg
     4021 tttggatagc gtgctatccc atatgacaga cggcgtcatt gcgacagatc gcaggggtaa
     4081 agtcattacg atcaatgata tggctgtttc cttgctagat atcaaaaacg aggaagcaat
     4141 cggacaatcg attttgacac ttttagatat cgaagaagaa tatacgttgc ggaaattatt
     4201 agaagatcca aacgaaatgg tacttgaccg ttcaaccagt acattggaaa gtgatcagat
     4261 gatcttgcga gtagactttt ctatgatccg tcgcgaatca ggttttatca gcggattagt
     4321 agctgtcttg catgatgtga cagaacaaga aaaaacagaa cgggaacgtc gtgaattcgt
     4381 ctctaatgtt tcccatgaac ttcgtacacc gttgacaagt atgcgtagtt atatcgaagc
     4441 attgagcgaa ggtgcgtgga aagacgagga agtagcacca aattttttga aggtaacgct
     4501 agatgaaaca gaccgaatga ttcgaatgat caatgacctc ttaaatctat ctcgcatgga
     4561 tacaggtaat acccagcttc aattagaata tgtcaatttc aacgagttag tgaattttgt
     4621 tcttgaccgg tttgatatga tggtgggaaa tcaggaaaaa aattataaga tccgtcgtga
     4681 atttacgcaa agagatcttt gggtcgaagt agatacagat aaaatcatcc aagtagtcga
     4741 taatatcatg aataacgcga tcaagtattc accagatggg ggaacgatta cttgtcgctt
     4801 attagaaacg cataataacg tggttttaag tatcaccgat caaggattgg ggattcctaa
     4861 aaaagatctg aaccgagtgt tcgagcggtt ctatcgagta gataaagcac gtgcaagagc
     4921 acaaggtggg acaggacttg gattagctat ttctagagaa gtgattaagg cgcatcgagg
     4981 cgctatttgg gcagagagca aggaaggaaa agggtcgact ttttatatct cgctgccata
     5041 tgaaccttat gaggaggaat ggtgggaatg agaatctctg aaaaagtcgt tcgtatagga
     5101 ctcattttga tggttgcgtt aagcatatac ttctcttatg caatctggct gagcccagca
     5161 ggaaaaagtt caatcaacgt agatgagaca aacactcaaa tgatcgagtc tcaaagcaat
     5221 cgaaaaactt ccgaagtctt tcttccgctt catgttacat ggcaccattc gggaatgatc
     5281 caagaaacca acagtgaaaa tctcgtgtac cgattgcaat tgatcatcga taaagcaaga
     5341 tttggaaagc tttctgaagt agtttcagga gacaaatcac agtttgaaca ggtgaaaaat
     5401 ctgaactctg gttttgagtt ggcctataat gcgccttttt tgctaagaga atacaaggaa
     5461 gcatttgatt tagatttaga tgtgtcgaac ttggcaaaag aaagcgaaaa tatgtatttt
     5521 actagaatac aattcgatca aggaaataaa aaaatccgat ttttgaatta ttcgaaccat
     5581 ctcgtatatg aagcaaatat ttcgttgaat tggacagata tcaataaaga attaacagca
     5641 aaagacacaa aatggacaga aatgacaaca gaaccatcga cgatcgacac gcaatatgct
     5701 acgaaacatt cgatcaaact gaaaaagtat agttatatcc tgtcacagca accctataca
     5761 ctttttagaa atgctttttt ccagaaacca gatagcgtga aaaataatga tgaaagcaat
     5821 gaattgattt tttacggtgg gaatgaaaca atgtctatcc attcggaaac gcaagtagtt
     5881 gattttcgtg gagagctgcc agagaaaaaa gaaaatgcta gtgtattttc tgaaagtttc
     5941 ccctatatta gcaaattagg cagttcttta gggaacctca gatattttga ccgcagtgac
     6001 aatacgatcg attatcggat attcgtggaa ggcttcccgg tttttgggac agacagcaga
     6061 ggacagattg gtgtggaagt tggcggtgaa acgaatggtt ctgtgcaagt gaatatccaa
     6121 accagtttga atacgattca agtgccgata ccttcagacg aagaagtaga acttgcaagt
     6181 acgaatgaaa tcatccagca gttaaaagct aagggagcag atagcaacaa gattggttca
     6241 gtgatcgttg gctatacatg gcaaaatgtc aaagaaacga atcgtgtcgt tgatctctta
     6301 cctgaatggt atgtgaaata taaagataac tggtattcag ctaacgacct tctggaaaaa
     6361 ttacctggat tggaggcgaa ttaattggat ttcaaaaaaa tcgaatggat atttttcgtc
     6421 gcttttttag ggttgaatgt gtttttattc agcagttatc atgacgcggt ataccaagaa
     6481 aacaatgtca ttcgctctaa tcagactgaa tcaatccaac aacgtctggc tagcgacgat
     6541 atcacttaca cggggaagat ttcgtctgag aacaagcaag gctattatct tagcgcggag
     6601 cagaccaatc ttagcacttc attagcgaat tatcgcaagg ctggtaaaag cggcttgctg
     6661 gcaagtggtg tgtttttaga cggtgatgtt ttgacgaaat ctttatccag ttcagatgct
     6721 gaaaagaacg tgatcgatcc agataaaatt agtagttcct tggaagattt tttatctaac
     6781 aaagacaata ttttattcgg taatgattat gtgtacacga aaaacttttc tcatttagaa
     6841 gaagaaccac tagaaatcca agcaagccaa gagtatgaag gcatcccatt caatgatgat
     6901 acagcaaaaa tcgttctttc tttagaaaaa aacgataatg attatagcat cacgaaatac
     6961 atgcagacgc atttatctga tatcgagcag ctgcgcgaaa agacggaact gtatacggaa
     7021 gaggaagcaa tcaatacatt gtacatcaat aacaaaatac caagaggatc aaaaatttta
     7081 tggcgtcagc tagcttattc ctgtattctg aaagtccggg aaaaaaatgt gtatgttcct
     7141 gtttggcatg tagcgatcga aacaagcgat aaagtggtcc aaattgagag tgtcaacgct
     7201 ttcagcaata cgatcgtgac aaacaatact gtaccaaaag tggaagatca ataaaaaagc
     7261 gagtataatg aaaaaagttt acaaagtaag aagggaagta ggagtcaaat ggctgaagaa
     7321 aaaccagctt ttaaaatcag tatcctagca agtggaagtt cgggtaattc actttatatc
     7381 gaaagtgaaa aaaaacggct tttggtcgat gcaggactta gcggtaaaaa aatcacttcc
     7441 ttgatggctc aaattgatcg gacaccggac caattagatg gaatacttgt gacgcatgaa
     7501 catcgagatc atatacatgg cgtgggtgtc ttggctcgta agtatcattt ggatgttttt
     7561 gccaatcagc agacttggga agcgatggaa ccactgattg gaaatgtacc agtggaacaa
     7621 aaacatctat ttgagatggg gaaagtcaga acattcggcg atatcgatat tgagagtttc
     7681 ggcgtctccc atgacgcagc ggctccgcaa ttttatcgtt tccataaaga aaatcggtca
     7741 ttcgtcatgt tgacggacac cggatattgc agtgattata tgcgaggtgt cattgaaaat
     7801 gccgatggtt atttgatgga aagtaatcat gatcttgaga tgttacgcat gggaccttat
     7861 ccgtggaatt taaaacagcg tattttagga gatagaggac atctttccaa cgaagacggt
     7921 gcacttgtca tgactgatgt gatcggtaat cggaccaaac gaatctatct tggccatctt
     7981 agcaaggaaa acaatatgaa agaacttgct catttaacaa tggaaaatgt tttgaaagaa
     8041 aaagattttg gcgttggaca tgattttgaa atctatgata cagatccaga tgaagcaact
     8101 gagctatttt cgatttaaac agttcaatca ataaaaatat aaaaagcaga agataaaatc
     8161 aatcagggtt ggttttatct tctattttta tttttttagc atattaaaaa aataaccgta
     8221 ttatcgactg actacactga tgtgctgctt ttcatgtttc tcatccagta tctcatctac
     8281 taatgcaatc gcgtaatctg ccatgctgat cgtactgtcg ccagcagcgt ttctttgtaa
     8341 ataatcatca cttaacaaat aagtacctgt acgtttgcca tgtggatcaa ataaagaagc
     8401 tggactgata taagtccaat gtagattttt agattgtttc aattttaaca atccttcgta
     8461 catattgaaa gctgttggat agaagggtgc atttttaggt acttggtcaa tcatacgagt
     8521 cgtcttttct gtatctaaga ataacgaaga ggcaccccca acgacaatta agcgtgtatc
     8581 ggttccttca agcatctcaa tcaaatggga caaagaagtt tgatgcattt cttcttttcc
     8641 tttgggagcg ttgaaagcat caacgacaac atcaaaagat gcaagatcag tagttgtcaa
     8701 ctcaaataaa tccttttgta taaacggaac atctgatggc aatttttctg gatgacgcac
     8761 gattgccgtc acgtctaaat ctctggacaa tgcttcgtct aaaataaatg aacctgcatg
     8821 gccagtagct ccaatgagtg caattttcat aaaaaacctc cttttttatt cctgtccatt
     8881 gtaagcagtt gctgaatatt cgtcaaagaa tccgattttt gaaaagctca gtgcgcattc
     8941 ctttcatggt tcgtgatcat tgggtatagt taaattaaga aaactgattg ataaaaatag
     9001 aagttgaagg agataagaaa tgaaagagtg gttagatcgt gcctttttac caatgataac
     9061 atctgttgtg aatcttagac ccttaactgc tttgcgtaat ggaatgactt acacgatccc
     9121 aataattatt gttggctcgc tgtttttctt atttgctcat tttcctgtgg attacatcgc
     9181 tcagtggata gacaaaacag gattttctat gtattttaca caagtatacc aggctacatt
     9241 tgatgtgata gccttttgga tcgtttttgc cattgcatac gcttatgcaa aagaagaaga
     9301 catcgatcgc ttaccagtcg gcataacagc cttgtctgct tatctactgc ttattcagcc
     9361 actaaatact cagatagata ctgtctggac aggtgctgaa gggatactag gagcagttac
     9421 aactgggctc atcatcggat ggggctatgc acgtttagta agacttgcga aagggtggaa
     9481 gcaaagagag aagatacctg aaaatgtttg gcagtctttt acttcattga ttccaatcat
     9541 tgttatagtc acttttacgc ttatcggttc agcggttttt caacagttgt tcagaatgac
     9601 tgttgttcaa ggagtatgga catatgtcca gcttcccatt caatcgttca tagacacgtt
     9661 gtttggtgtg cttttattag gtttttttgt tccgttttta tggttatttg gtcttcatgg
     9721 atctgcgatg gtcaatgggt tggttagtcc cgtcttgcaa gcgaattcat tagcgaatgc
     9781 agaaatctta gcctcaggac aagagttgac ggtggcaaat ggcggacata tcgttactca
     9841 gcaatttctc gatcagttca tgacggttac gggtgctgga ttgacgttag gggctgtatt
     9901 ttttatgacg gtttttgcga aatcgaagaa atacagagaa ttaggaaatc ttgctctttt
     9961 accagccttt ttcaacatca atgaatcaat tattttttcc acaccgatcg tgatgaaccc
    10021 gatgatggct gttcctttta ttttcgcacc ggtcgtttca ggattgatta cctattccgc
    10081 tttatatttt ggattcgttc ctttatttac agcagtccaa gtcccttgga caacaccgcc
    10141 gatcctctcg ggtttcttag taggcggagt gcctgcagcg attttacaag gtatcgtgtt
    10201 gtcaattagt tttttcattt atttcccatt tttgaaaaaa atagattctg caaactgcaa
    10261 aaaggaaaga cagacaaaaa atgatggagc agcagaaaag atgaacgact aaaaaacaaa
    10321 aatactcctg tcgctttaga ttgggaaaat caccaatcta ggatgacagg agtatttata
    10381 gtcatgttta tactagccct ttttggacag ctaattgaac aagaacaact aatgtgttca
    10441 ttcctacgtg tgtgatcatc gatgtccaga tccgaccagt tgctttgtac tctaggctga
    10501 agaagaaacc taggaaaaag tagattagca gatgaccatc attatgagca aacgcaaaca
    10561 gcaaagagct gatcactgct ccgacccaaa aatttgaata tcgactgatg atccccagta
    10621 tactgcggcg gaatacgaac tcttccatga tgggtccagc aacagttgtc gccaaaataa
    10681 aagcgggtgc ttccatgatc atctggatga tattttgtgt attctcggaa ggggttgctt
    10741 tgccaaacaa tacagattct aacatgacag cgatgctttg cagaatcaat gcgacaaaga
    10801 ttccaaccaa tccataaata acaatctttg gagcagaagc tttttttacc gctgattcga
    10861 tcgctaaggg ttcacgttgc ttaaaataaa aaatagctag gacaacggcg cctagtatat
    10921 acatgactgc agttgctgtg atcaattcag acgtagattt tacaagtcct atcgaagcta
    10981 ggatcaaggg tgagataaaa attagaccat agcaaaaaat gctgaggaag ctatattttt
    11041 ttaaagacat gtaattcctc cttttaaggg ataacaagca tgcaaggcaa gcttatatcg
    11101 attgatttca attatatcat aatttcaata agcagctgtc agaataaaat ttcttttctt
    11161 tgatttaaaa gcttgcaaaa aagagggaga atgagtatta ttaaaaatgt aggttagcac
    11221 tcgaaggtta agagtgctaa cagattaaaa aaagagttat cggagggatt tatcgtgtta
    11281 aaaccattag gtgatcgcgt cattatcgaa gtcgcaaaag aagaagaaaa aactgtcgga
    11341 ggaatcgttt tagcttctgc tgctaaagag aaaccccaaa ctgggacagt tgttgcagtc
    11401 ggtgaaggcc gtttgttaga aaacggtgaa aaagttcctg ccgctgtaaa agcaggcgac
    11461 caagttatgt tcgaaaaata cgccggtaca gaagtaaaat acgaaggaaa agaatatctg
    11521 atcgtcgcag gaaaagatat catggcgatt gttgagtaaa tttggaagat aaaaaaataa
    11581 tcaaaaataa tcaatgaggt gaatcaatca tggcaaaaga attgaaattt gcagaagacg
    11641 cacgcgcagc tatgttacgc ggggtagata aattagcaga tacagtgaaa gtaacattag
    11701 gacctaaagg ccgaaatgtc gtactagaaa aatcttacgg ctctccattg atcacaaatg
    11761 atggtgtgac gatcgcaaaa gaaatcgaat tagaagatca ttttgaaaac atgggtgcaa
    11821 aacttgtatc agaagtagca tcaaaaacaa atgatattgc aggagacggt acaacgacag
    11881 caactgttct gacacaagca atcgttcgcg aaggtttaaa aaacgtaact gcaggtgcta
    11941 atcctctagg cattcgtcgc gggatcgaat tggcaacaaa agcagcagtc gaagaattgc
    12001 ataatatctc aactgttgtt gattcaaaag aagccattgc acaagtagca gctgtttctt
    12061 cgggttctga taaagttgga catttgattg ctgatgcaat ggaaaaagtt ggaaacgacg
    12121 gtgtcatcac aatcgaagaa tcaaaaggga tcgaaacaga actagatgtt gtagaaggta
    12181 tgcagtttga tcgtggttac ttatcacaat atatggtaac agataacgac aaaatggaag
    12241 ctgttctaga aaacccatat atcttgatca cagacaaaaa aatctctaac atccaagata
    12301 ttctgccatt gttagaacaa atcttgcaac aatcacgtcc attgttgatt attgctgacg
    12361 atgtagatgg tgaagcattg ccaactcttg tattgaacaa aatccgtgga acattcaacg
    12421 tagtagctgt aaaagctcct ggtttcggcg atcgtcgtaa agcaatgctt gaagatatcg
    12481 ctattttgac aggtggtaca gtaattactg acgatctagg tttagaattg aaagatgtaa
    12541 caatcgaaaa cttaggaaat gcttctaaag ttgttgtaga taaagacaat acaacgatcg
    12601 tagaaggttc tggagaaaaa gaagcaatcg aagctcgtgt gcaattgatc aaaaaccaaa
    12661 ttgctgaaac aacttctgat tttgaccgcg aaaaattaca agaacgctta gctaaattag
    12721 caggtggtgt agctgttgtt aaagttggtg cagctactga aacagaattg aaagaattaa
    12781 aattacgcat tgaagatgca ttgaatgcta cacgtgccgc agtagaagaa ggaatggtct
    12841 caggtggtgg tacagctcta gtgaacgtta tcagcaaagt ttctgctgta gaagcagaag
    12901 gagacgtggc aacaggtatc aagatcgttg ttcgtgcttt agaagaacca attcgtcaaa
    12961 tcgctgaaaa cgctggttat gaaggatcag tgattgttga taaattgaag aacgtggaac
    13021 taggcactgg tttcaatgct gctactggag aatgggtaaa catggttgaa gcaggtatcg
    13081 tagacccaac taaagtaaca cgttctgcct tgcaaaatgc cgcttccgtt tctgccttgt
    13141 tattaacgac agaagcagtc gttgcagata aaccagaacc agcagcgcca gcagctcctg
    13201 caatggatcc atcaatgatg ggcggtatga tgtaagaaaa agcaacttgc ttatctacaa
    13261 ctgatcatta gtgaaagaca gataaaaaga ggctgg
//
LOCUS       DACENJ010000065        13199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.065, whole genome shotgun sequence.
ACCESSION   DACENJ010000065 DACENJ010000000
VERSION     DACENJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.065"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(130..825)
                     /locus_tag="IW748_001332"
     CDS             complement(130..825)
                     /locus_tag="IW748_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1104250.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IW748_001333"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IW748_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1104251.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="IW748_001334"
     CDS             complement(2024..2215)
                     /locus_tag="IW748_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1104252.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="IW748_001335"
     CDS             complement(2236..3126)
                     /locus_tag="IW748_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1104253.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="IW748_001336"
     CDS             complement(3113..3880)
                     /locus_tag="IW748_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1104254.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IW748_001337"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IW748_001337"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1104255.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IW748_001338"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IW748_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1104256.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IW748_001339"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IW748_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1104257.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="IW748_001340"
     CDS             8496..8834
                     /locus_tag="IW748_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104258.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="IW748_001341"
     CDS             complement(9002..9760)
                     /locus_tag="IW748_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1104259.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="IW748_001342"
     CDS             complement(9778..10596)
                     /locus_tag="IW748_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1104260.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IW748_001343"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IW748_001343"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1104261.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IW748_001344"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IW748_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1104262.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="IW748_001345"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="IW748_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1104263.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
BASE COUNT         3597 a         2825 c         2365 g         4412 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattac
//
LOCUS       DACENJ010000066        12993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.066, whole genome shotgun sequence.
ACCESSION   DACENJ010000066 DACENJ010000000
VERSION     DACENJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.066"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..116
                     /locus_tag="IW748_001346"
     CDS             <1..116
                     /locus_tag="IW748_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAQ1104264.1"
                     /translation="SEFSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(154..1302)
                     /locus_tag="IW748_001347"
     CDS             complement(154..1302)
                     /locus_tag="IW748_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1104265.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(1424..2020)
                     /locus_tag="IW748_001348"
     CDS             complement(1424..2020)
                     /locus_tag="IW748_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1104266.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2220..4862)
                     /gene="alaS"
                     /locus_tag="IW748_001349"
     CDS             complement(2220..4862)
                     /gene="alaS"
                     /locus_tag="IW748_001349"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1104267.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5250..5681
                     /locus_tag="IW748_001350"
     CDS             5250..5681
                     /locus_tag="IW748_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1104268.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5726..7075)
                     /locus_tag="IW748_001351"
     CDS             complement(5726..7075)
                     /locus_tag="IW748_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1104269.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7145..8308)
                     /locus_tag="IW748_001352"
     CDS             complement(7145..8308)
                     /locus_tag="IW748_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104270.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8437..8760
                     /locus_tag="IW748_001353"
     CDS             8437..8760
                     /locus_tag="IW748_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104271.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8943..10292)
                     /locus_tag="IW748_001354"
     CDS             complement(8943..10292)
                     /locus_tag="IW748_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1104272.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10361..11314)
                     /locus_tag="IW748_001355"
     CDS             complement(10361..11314)
                     /locus_tag="IW748_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1104273.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11416..12606)
                     /locus_tag="IW748_001356"
     CDS             complement(11416..12606)
                     /locus_tag="IW748_001356"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1104274.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3747 a         2728 c         2237 g         4281 t
ORIGIN      
        1 tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat aaaggagcag
       61 ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact ctttaaatta
      121 aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat tgtgatcaaa
      181 cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta agattctttc
      241 tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt gcagcgtttt
      301 tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt caagaagaat
      361 ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca ttggatccaa
      421 gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca gatcaataat
      481 cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg atgcaaagcg
      541 tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat catcgcaagc
      601 atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc ctaatagttc
      661 gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg ctaaagcaaa
      721 acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg aaagcaatac
      781 agccagtgta gtaaagacat cattataact atctgttgct gtagctctca atgtttcaga
      841 atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca aaaagatgga
      901 aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat ggatgatttt
      961 ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg aaacaaataa
     1021 cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg ctggctttgc
     1081 agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata gactgttcac
     1141 tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct tagagacaaa
     1201 caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt ttgtccgctg
     1261 ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta ccccttctag
     1321 tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc ctctcttcct
     1381 ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct ggtttcaacg
     1441 gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca cctattgctt
     1501 ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga gcagagtcta
     1561 caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct aacactaaga
     1621 ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt attgatttat
     1681 ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt actctgggaa
     1741 ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt ggcatggcat
     1801 aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca tcttttgggc
     1861 ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta atgaatccta
     1921 atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct aacaagatcg
     1981 cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc ctttttattt
     2041 atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga atccgaccat
     2101 ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt tgtcttcgcc
     2161 cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac gtaaacgccc
     2221 tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc agcaggattt
     2281 ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac tttcggtgcg
     2341 attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt cgtcattgcc
     2401 gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc tgaaatattt
     2461 ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac ttttacttgt
     2521 gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc accagcttgt
     2581 tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct tacttgctgt
     2641 tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac aattgttgct
     2701 acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt acttgttaca
     2761 gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt gaagattccg
     2821 atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca gccgccaata
     2881 tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc acccatgttt
     2941 ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca gattttttcg
     3001 ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa gtgggtaaag
     3061 tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg ctcgccaagt
     3121 acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat gatccgattg
     3181 cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg tgcttttact
     3241 tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac aactgctacg
     3301 atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc catttctgca
     3361 tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc attcaaaaga
     3421 gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt gtatccaatg
     3481 aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat tgatttttca
     3541 gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc aaaaccagca
     3601 tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac cgggaagcca
     3661 tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt ttcgtttttt
     3721 acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc attgaaacgt
     3781 tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac ttcaggataa
     3841 taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc ttgttcaata
     3901 cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac ataccctctc
     3961 ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg aatatgatct
     4021 gcgattactt tgtaagaaat atctgttact gggttttcac catattttac ttgtttgctt
     4081 agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt cgttgacgca
     4141 tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt tttatgagga
     4201 agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa gttccagatt
     4261 tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc tgctaaatcg
     4321 ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg gcctgcaccg
     4381 atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc aacttcttca
     4441 tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag tttttctgga
     4501 tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat ggcttcattt
     4561 ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg gtgccgtgct
     4621 gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt tgtgattctt
     4681 ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac accagagttg
     4741 atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc aatcgtatgt
     4801 cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact taattgtttc
     4861 atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag ccaaggacga
     4921 aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta aactcgataa
     4981 cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg attagtgact
     5041 attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat catatccttt
     5101 tcgtacttat ttagtatacg cattctattg atcactgtca atatctcaat ttttattttc
     5161 atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga atcaatacat
     5221 ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg gatgaatgga
     5281 tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa gggattcctg
     5341 cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta tttcttgatc
     5401 aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc ttcaagccgg
     5461 aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag ttgcttgaag
     5521 ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta gctgaaatga
     5581 cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct tttattgaag
     5641 atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag attgtgacac
     5701 gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt ttacgagcag
     5761 tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct gcaattgccc
     5821 aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc acaagaccaa
     5881 tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta ttacgatcgt
     5941 atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg cccaattttt
     6001 ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc gttcctttta
     6061 agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct tctggaattt
     6121 ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct aaatcagtcg
     6181 ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt ctctctcttg
     6241 gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag taatcggcaa
     6301 tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga tgaccaattg
     6361 tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc agccaatttt
     6421 caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga ttttccatgt
     6481 attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact aaaaactgca
     6541 gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc atatccaatg
     6601 tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc gcttgttcat
     6661 tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt tggtgatgca
     6721 gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca cggatctccg
     6781 gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct agttccctac
     6841 ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt tcaaacaaag
     6901 gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg atattttttc
     6961 cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg aagccctttg
     7021 cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta ttcattcgtg
     7081 cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa gcgtttttgc
     7141 tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca gtatatgtgt
     7201 gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc aagatattta
     7261 aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca ctcatctgca
     7321 acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac cagcttaaac
     7381 cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa gaaattcgtt
     7441 cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc catccattgg
     7501 ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac cagatatgag
     7561 aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc aaattaataa
     7621 gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat gagacagtct
     7681 ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata aatgtaaata
     7741 acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa tcattcacat
     7801 caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc gtcaattcaa
     7861 aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc cggaaataat
     7921 atcttaaata aatgccaaat ggtatgagta ataaaacatt aaaagctggt tccaaaaata
     7981 cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat accgtgttgt
     8041 ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct gtcatttggg
     8101 caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac aataaataaa
     8161 aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac tttcgatatt
     8221 cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca atcagcgcaa
     8281 aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg tatctctctt
     8341 atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct catttctttc
     8401 aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta ctttgttttt
     8461 ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag aaaaagaact
     8521 acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct agtcttcaat
     8581 cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca tgctttgtct
     8641 aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc tggttgcttg
     8701 gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa atcaaagtaa
     8761 aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca tcgtagacaa
     8821 aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca cgtactcctc
     8881 atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt cgaatgtttt
     8941 agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa tactatcccc
     9001 atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt ccctccaatg
     9061 attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa caaatcaggt
     9121 tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat ccaaatgaca
     9181 aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc aatagcgatg
     9241 actagctcta agatacctgc ggctacagga actttagttt tatcatgaag tttagcaaag
     9301 agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg cattccagta
     9361 agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt gaccagtctt
     9421 ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct gtttcctgcg
     9481 actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata aacgatcatg
     9541 attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt tttcaattct
     9601 cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc tagcaagccc
     9661 gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa cagttggaag
     9721 tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg aataagcttg
     9781 cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa attgatcaac
     9841 aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga aagaccaaat
     9901 agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc tggaaaataa
     9961 atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat ccgttcgata
    10021 tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc tgttagtcct
    10081 gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact ggcggatcct
    10141 gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac tgtgcccata
    10201 acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc tatttttact
    10261 tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata ccatagccaa
    10321 tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc tctaccaatt
    10381 tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat ttcggcgaaa
    10441 agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat tctgctattg
    10501 actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt ttttgaatag
    10561 atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca tttcgatgaa
    10621 ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca tctggcatat
    10681 acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct tctttgattg
    10741 ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag gttgcataag
    10801 aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca taataataat
    10861 cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga cgctgaagag
    10921 tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt gaacttggcg
    10981 taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc ccagtcacgt
    11041 tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga gcagccaaat
    11101 gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg taatccatta
    11161 catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt atatctgtcg
    11221 caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct gaaccaattt
    11281 gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat cttttttact
    11341 actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt aaatagacga
    11401 aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat aaactgctac
    11461 tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga tcctgcctgt
    11521 cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt cgatcagctt
    11581 cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa taggtgtttc
    11641 agacataccg atgttatagc ctactttttt caattcttct ttaaaataat tagcattttc
    11701 ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag ccaatgcagc
    11761 tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc tagctttcaa
    11821 ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta tagctttcga
    11881 taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga cggttcctgc
    11941 accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca gtccatattt
    12001 ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg agaaaacacc
    12061 atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct ttgctttttt
    12121 ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg ataagcggca
    12181 accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt cttttgtcat
    12241 tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga ttgctgcttc
    12301 tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca gcgaaccatt
    12361 gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa tacatgcttt
    12421 tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat tgatcatctt
    12481 ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga tcgtattgta
    12541 taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta gttctttgga
    12601 agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac atatatcgtg
    12661 tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg attatgagaa
    12721 ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact tatatagtat
    12781 gaacaattac cttttagata atgatctatt ttactctacc tgggcagata aatacaacac
    12841 aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt aatttattta
    12901 ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca atttttaatt
    12961 ttactctctg aatatttctt aaaagtttta ctt
//
LOCUS       DACENJ010000067        12841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.067, whole genome shotgun sequence.
ACCESSION   DACENJ010000067 DACENJ010000000
VERSION     DACENJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.067"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1130
                     /gene="dnaN"
                     /locus_tag="IW748_001357"
     CDS             <1..1130
                     /gene="dnaN"
                     /locus_tag="IW748_001357"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1104275.1"
                     /translation="KVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTLT
                     GSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQV
                     AITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPIL
                     TGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEEI
                     VEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERAS
                     LLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKAA
                     LRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            1359..1601
                     /gene="yaaA"
                     /locus_tag="IW748_001358"
     CDS             1359..1601
                     /gene="yaaA"
                     /locus_tag="IW748_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1104276.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            1588..2712
                     /gene="recF"
                     /locus_tag="IW748_001359"
     CDS             1588..2712
                     /gene="recF"
                     /locus_tag="IW748_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1104277.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            2709..4655
                     /gene="gyrB"
                     /locus_tag="IW748_001360"
     CDS             2709..4655
                     /gene="gyrB"
                     /locus_tag="IW748_001360"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1104278.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            4675..7146
                     /gene="gyrA"
                     /locus_tag="IW748_001361"
     CDS             4675..7146
                     /gene="gyrA"
                     /locus_tag="IW748_001361"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1104279.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            7382..7681
                     /locus_tag="IW748_001362"
     CDS             7382..7681
                     /locus_tag="IW748_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1104280.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            7731..8243
                     /gene="ssb"
                     /locus_tag="IW748_001363"
     CDS             7731..8243
                     /gene="ssb"
                     /locus_tag="IW748_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1104281.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            8269..8505
                     /gene="rpsR"
                     /locus_tag="IW748_001364"
     CDS             8269..8505
                     /gene="rpsR"
                     /locus_tag="IW748_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1104282.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            8648..10621
                     /locus_tag="IW748_001365"
     CDS             8648..10621
                     /locus_tag="IW748_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1104283.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            10627..11079
                     /gene="rplI"
                     /locus_tag="IW748_001366"
     CDS             10627..11079
                     /gene="rplI"
                     /locus_tag="IW748_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1104284.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            11353..12720
                     /gene="dnaB"
                     /locus_tag="IW748_001367"
     CDS             11353..12720
                     /gene="dnaB"
                     /locus_tag="IW748_001367"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1104285.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         4298 a         2303 c         2810 g         3430 t
ORIGIN      
        1 tgaaagttac tttaaaccga gctagcttta tgcaggaatt gcaaactgtt caacgagcta
       61 tttcaagcaa aaccacgatc cctattttga caggtgtaaa aatcacactg acacaagaag
      121 gtttgacttt gacggggagc aacgctgata tatcaattga aacttttttg tctgttgaaa
      181 acgaaaaagc aaatatgcaa atcgaatcta ctggttccat tgttttacaa gcacgtttct
      241 ttagcgaaat cattcggaga cttcctgaag aaacatttac tttagaagtt ttagaaaata
      301 aacaagtagc gatcacttct ggaaaagcga attttatcgt aaatggatta gatgcagata
      361 actatcctca tcttcctgtt gtcgaaagcc ataaccagat gaaattacct gtacacgtat
      421 tgactaaact aatcaacgaa acagtttttg ctgtctcaca acatgagagt cgtccaatct
      481 tgacaggtgt gcatttcatt ttatctgata attctttatt agctgtagct actgattctc
      541 accgtctaag tcaacgcgtg attccagtag aacaagcggc tgatcatttt gatattgtta
      601 ttcctggaaa aagtttgatc gaattatctc gctcattaac aaatgaagaa gaaatcgtcg
      661 aaatcagcat tatggaaaac caagtgttgt tcaaaacaga aacgatgtat ttctattccc
      721 gtttgctaga aggaaactat ccagatacca atcgtttgat tccttcaagt tttaatacag
      781 aggttgaatt ttctgttcct agctttttag cggcgatcga acgggcttct ttactttctc
      841 atgaaggccg taacaacatc gttcgtttat ctattcgtcc agatgcggtt gttttatacg
      901 gaaattcgcc agaaatcggg aaagtagaag aaagcttgag ttatacagca agcagtggcg
      961 atccattaga tatttctttc aatccggatt atatgaaagc agcgttgcgt gcatttggag
     1021 atatgagcat caaagtgaaa tttatctctg ctattcgtcc atttacattg gaaccaacgg
     1081 aagatggcgt tcaatttatt cagctgatta ctcctgtacg tacaaattaa caatgatttt
     1141 caatgttctt gaaaatttaa ttgaaaagaa atagaaaaaa taaaatttta aacagaaggc
     1201 cctaaaatct cttttaaggc tttctgttta tttttgttta aatcaccttt tttagattaa
     1261 atcgtcttat atggaaaaat atatggataa atttgtttta ttgcgaaaaa aaaggtataa
     1321 tagagtatac ttttaataat gaaaatgagg gatgggcatt gaaacaacaa gtcattctaa
     1381 atacggaatt catgacgttg ggccaaatgt tgaaagaagt cacagttatc ggtagtggcg
     1441 gacaagcaaa atggtatttg gcagaaaaca ctgtgttagt cgatggcgaa ccagaaaatc
     1501 gtcgtggacg caaactttat cccggcatga tggttgaagt tccagaagta ggaacttttt
     1561 ttatggtaaa aaaaggaaac gacgataatg aggctgaata agctgtattt aaaaaattat
     1621 cgaaattacg aagagctgaa catcgaattt tccaagaacc tcgtcatctt tttaggagaa
     1681 aatgcgcaag gaaaaacgaa tcttttggaa agtatctacg tattagctat gacgcgaagt
     1741 catcggacaa gtagtgagca ggaattgatt ggctggactg acgatcaggc gatgattcaa
     1801 ggagagatca caaaaggttc atccactctt ccattagaaa tcttattatc taaaaaaggc
     1861 cgaaaaacca agatcaacca tatcgaacag aaaaagttaa gtacttacgt gggacaaatg
     1921 aatgtcattt tatttgcacc agaagatctt tctttagtaa aaggcagccc gcagaccagg
     1981 cggaagtttt tagatatgga attaggccag atcaatccaa tctatcttta tgatcttgtc
     2041 caataccaag caattttgaa acaaagaaac cagtacttga agcaattgaa tgaaaaaaaa
     2101 caaactgatt tgctttattt ggatattttg tccgaacaac tagcagcgtt tggcagcaaa
     2161 gtattgaaag caagagcaca gttcgtcaag cgattggagt tttgggctaa ttctttgcat
     2221 cagcaaatca cccatcaaaa agagcagctg gaaatcgagt atctcaccgc agtcgattct
     2281 ttagaaacac atacgcaaga gcaaatccaa gaacaatttc tcgcactttt gaaccaaaat
     2341 aaaaagaaag acctttttcg gggaacaacg acagttggtc ctcatcgtga cgatctttcc
     2401 ttttttatta atcagaaaaa tgtgcagact tatggatcgc aaggacagca aagaacaact
     2461 gcactaagtg tcaaattagc agaaatcgat ttgattaaag aagagacagg ggaatatcca
     2521 atccttttgt tagatgatgt gatgagcgag ctggatgata atcgtcaact ccatctgcta
     2581 gaaacaatcg aaggaaaagt tcaaacgttc ttaacaacaa cgacacttga ccatgtaaaa
     2641 aataaaatga ctgttgaacc tgagattttt tatgttcaac aagggaaact aaaaggagaa
     2701 gaaactgaat gacagaagaa agaagtttag tagaacgcgc taaagagtat gatgccagtc
     2761 agattcaagt acttgaagga cttgaagccg ttcgtaaacg acctggcatg tatatcggtt
     2821 cgactagttc tgagggtctg catcaccttg tttgggaaat tgtcgataat tcgatcgatg
     2881 aagtattagc aggttttgca acgaaaatcc atgtgattat tgaaaaagac aacagtatca
     2941 ccgttattga cgacggtcgg gggatacctg tagatatcca ggcaaaaact ggacgtcctg
     3001 cagttgaaac ggtctttact gttttacatg ctggaggaaa attcggcggt ggtgggtaca
     3061 aagtttctgg tggacttcac ggagtaggat cttctgttgt taacgcgctt tctacacagc
     3121 ttgatgtcaa agtatacaaa gaaggaaatg tgtactatca agaatatcgt cgtggagctg
     3181 tagtggacga cttaaagatt atcgaacaaa cggatcgcca tggaacaacg gttcacttta
     3241 cgccagaccc tgaaattttt acagagacaa ctgagtttga tttttcaaaa ttggctaccc
     3301 ggatccgtga acttgctttc ttgaaccgcg ggatgcgtat atcgattgaa gacaaacgag
     3361 aagaagaacc agtcatcaat gaataccatt atgacggcgg gattaaaagt tatgtcgaat
     3421 acttgaatgc aaacaaaacc gtgatttttc cagaacctgt atatttggaa ggtgaacagc
     3481 aagatatcgc agttgaagtc tctatgcaat atacagacgg ctaccattcc aatatcatga
     3541 gttttgccaa caatatccat acgtatgaag gcggaacaca tgaatcagga ttcaagactg
     3601 ctttaactcg tgtgataaat gattatgccc gaaaacaaaa gctgatgaaa gaaaatgacg
     3661 acaacttgac cggtgaagac gtgcgagaag gattgacagc tgttatttct atcaagcatc
     3721 ctgaccctca attcgaagga caaacaaaaa caaaattggg caattccgaa gtacggacag
     3781 tcactgatcg gttatttagc gaacatttca tgaaattctt gctggaaaac ccaagtgtgg
     3841 gccgtcaaat tgttgaaaaa ggattactag cttccagagc tcgtctagca gcgaaacgtg
     3901 cgcgtgaagt aacgagacgt aaaggtgctt tagagattag taatctgcca ggaaaattag
     3961 cggactgctc tagcaacgat cctgaaaaat gcgaattgtt catcgtcgaa ggagattcgg
     4021 ccggcggatc agctaagcaa ggtcgtaatc gagagtttca agccatcttg cctattcgtg
     4081 ggaaaatctt gaatgttgaa aaagcaagca tggacaagat cttggcaaat gaagagatcc
     4141 ggtctttatt tactgctatg ggtacagggt ttggagaaga ttttgatgta tcgaaagctc
     4201 gctaccacaa attagttatc atgactgatg ccgatgtcga cggcgcgcat atccgtacat
     4261 tgctgctgac tcttttttat cgttacatgc gtcctattgt tgaagctgga tacgtctata
     4321 ttgctcagcc acctttatac ggggtaaaac aagggaagaa tattacgtat gtacaaccag
     4381 gaaaaaatgc agaagaagag ttgaaacaag tcgttgcttc acttcctgcc tcaccaaaac
     4441 caagcgttca gcgttataaa ggacttggag aaatggatga tcatcaacta tgggaaacaa
     4501 ctatggaccc aagcaatcgg atgatggccc gcgtcagtgt ggacgatgct attgcagcag
     4561 atcagatatt tgaaatgttg atgggggatc gagtagaacc ccgtcgtgca ttcatcgagg
     4621 aaaatgcaca ctatgtgaag aacttagata tttaatcatg aaggaggaaa aagaatgagt
     4681 gaagaaatca gagaaaacat ccatgatgtc aatctaacca gcgaaatgaa agactccttc
     4741 atcgattatg cgatgagtgt tattgtcgca cgtgcacttc ctgatgtcag agatggtctg
     4801 aaacccgttc atcgccgtat tttatacggc atgaacgaat taggtgtaac accggataaa
     4861 gcccataaaa aatctgccag aatcgtcgga gatgtcatgg gtaaatacca tcctcatgga
     4921 gactatgcga tctatgagtc aatggtacgg atggcacagc cattcagcta ccgttatatg
     4981 ctagtggatg gtcatggaaa ctttggttct gttgacggtg acggtgccgc cgctatgcgt
     5041 tataccgaag cacggatgag caaaatcgct acagaaatgc tgcgtgatat caataagaac
     5101 actgttgatt tccaaagcaa ctatgatgat acagaaaaag aaccagttgt actacctgct
     5161 cgcttcccga atcttttagt gaacggaaca acggggatcg ccgtaggtat ggccacgaat
     5221 atcccacctc ataacttgtc tgaggtagta gcggctatcg atttgctgat ggataatcct
     5281 gatgtgacga ccaatgaatt gatggaagta ttacctggac cagattttcc aacaggtggt
     5341 ttggttatgg gaaaatcagg aatccgaaga gcgtatgaaa caggtaaggg ttctattacc
     5401 gtacgtgcaa aagttgagat tactgagatg ccaaatggta aagaacggat catcgtgaca
     5461 gaattgcctt atatggtcaa taaagcaaaa ttgatcgaac ggatttctga actgcacaga
     5521 gacaagcgta tcgaagggat cacagatttg cgggacgaat cttcccgtga aggtatgcgg
     5581 atcgtcattg acatccgtcg tgacgccagc gcatctgtta tcttgaataa cttatataaa
     5641 ttgacatcct tgcaaaattc atttggattc aatatgttgg cgatcgaaaa aggtgtgccg
     5701 aaagttttga gcttgaaaca aattcttgaa aactatatcg aacatcagcg cgaagtcatc
     5761 acaagacgga cagtctttga aaaagaaaaa gcagaagcaa gggcacacat tttagaaggt
     5821 ttgcgtattg ctttggatca tatcgatgaa atcattgcga ttatccgcgg gtctaaatca
     5881 gatgacgaag caaaagcgac gatgatcgaa cggttcgatt tatccgatcg ccagtctcaa
     5941 gctattttag atatgcgttt acgccgattg accggtttgg aacgggaaaa aatcgaaaat
     6001 gaataccaag aactcttaaa actgattgaa gatttaactg acattctggc aagaccagaa
     6061 cgtgttacag aaatcatcaa gaatgaatta gcagacttga atcaacgatt tggtgataaa
     6121 cgacgcacag aattactagt cggtgaggtc ttaagtctgg aagatgaaga tttaatcgaa
     6181 gaagaagaaa tcgttattac attgacaaac aatggttaca tcaaacgttt ggcaaataat
     6241 gaattcagag ctcaacgaag gggtggacgt ggtgtccaag gaatgggtat ccacaatgac
     6301 gacttcgtca aaaatctggt ttcttgttct acccatgata cattgttatt ctttacgaat
     6361 aacggaaaag tgtatcgggc aaaaggatac gaaattccag aatacgggcg cacagccaaa
     6421 ggaataccaa ttatcaattt actaggtatc gattcgagtg aaagcatcca ggcaatcatt
     6481 gcggtgacgg gagaagcaga agaaggccac tatctcttct ttacgacgcg tcaaggtaca
     6541 gtaaaacgta cttctgtcac tgcttttgct aacatccgaa gtaatggtct gatcgcaatt
     6601 ggtttgaaag aagacgatga gctcgtcaat gtgctactga cagatggtca atctaatatc
     6661 attattggaa cacataacgg ctactctgtc actttcaaag aagaagcagt ccgcgatatg
     6721 ggacgtacag cttccggggt tagaggaata cgcctgcgtg aggaagatta tgtcgttggg
     6781 gcagccttaa tgaaggaaga tcaagaagta ttagtgatca cagaaaaagg ttacggaaaa
     6841 cggacaaaag tatcggaata tccagtgaaa ggccgcggag gtaaagggat caaaacggcg
     6901 aacatcactg aaaaaaatgg tccactagct ggattgacca ctgtttctgg agatgaagac
     6961 atcatgctga ttacagataa aggtgtcatt atccgtttca acgtatcgac tgtttctcaa
     7021 actggacgtt caacattagg cgtacgtttg atgaagatgg aagctgatac gaaagttgtc
     7081 acgatggcag cagtcgaacc agaagctgca gaacaaacag ttgaagaaca aggatcagaa
     7141 gaatagccat caagactaat tgtgtaagct agcaagaatg accgattaaa aaatctggtt
     7201 attcctgctg gcttttttga ttagttgaat ttctcttaaa atattcgaaa ctgttcattc
     7261 tgtattgctt ttgaccaaaa tagagtgtat aatcagagct tgtgagtagt ctaattaatt
     7321 agataactct ccttgctctt aggaacctaa gagccgaaag tccacaggga ggtgaatatc
     7381 aatggaaaac acgaaatatg aaatcatgta cattattcgt ccaaacattg atgaagaagc
     7441 aaaaactgct ttaatcgaac gtttcgatgc aatcttgaaa gataacggag cagaagttat
     7501 cgaatcaaaa gattgggaaa aacgccgttt cgcttatgaa atgaacggtt accgtgaagg
     7561 tatctatcac atcgttaaag tatcttctcc atcatcagca aatgctgtca atgaatttga
     7621 ccgtcttgct aaaatcaacg acgatatcat tcgtcacatg attgttaaag aagaagctta
     7681 attgttgttt catgtgaaac aatagaaaac gatgagagga gatgactatt ttgattaata
     7741 atgttgtatt agttggacgt ctgacgaaag atccagattt gcgttacaca gcaagtggta
     7801 cggcagtcgc aacatttact ttggctgtga atcgtaattt tacaaaccaa agcggcaatc
     7861 gcgaggctga ttttatcaac tgtgtgattt ggcgcaaatc agcagaaact ttagcaaact
     7921 atgctcgtaa aggaacactt ttaggtgtta ctggacgtat ccaaactcgt tcttatgata
     7981 atcagcaagg acaacgtgta tacgtgacag aagtagtagc tgacaacttc caattgctgg
     8041 aaagtcgttc tgcatctgaa aatcgccaac aaagcggcgg attccaaagt tctggtcaat
     8101 cagctggcgg atttggcgga aacaataact ctaatcaaac atctcaatca tccaacggta
     8161 tgcctgattt cgaccgtgat acttctgacc cattcggctc aagttcaacg atcgacattt
     8221 cagacgatga tttaccattc taaacgaatt tgataggagg gaaatagaat ggcacaacaa
     8281 agaagaggcg gacgtaaacg tcgtaaagta gactacattg ctgctaacca tattgaatat
     8341 atcgattata aagatatcga attactaaaa agatttattt cagaacgcgg taaaatcttg
     8401 ccacgtcgtg taactggtac aggcgctaaa aaccaacgta aaattactgt tgcaatcaaa
     8461 cgcgctcgta tcatgggctt gctacctttc gttagcgaag actaataaat cctaggaatt
     8521 gaatgactga tccttttgat cagctattca attccttttt ttctgtaaaa ataacctcta
     8581 ttcgtttagt cgctcgaatg acgaaagtta tggtaaaatc aaattagtca tatatgggag
     8641 gaaaattatg gaaaataaaa acaatcattt atccccgaaa cctctcttcc ttatcctgtt
     8701 tatattaatc gaagtattgg tagtcttaat tttaccattt cgttttgtta gcgcttcggt
     8761 cattctattg atgaatttat ggggtgttcg ctattggttg aaaatgaaac gttggcttat
     8821 tttaaataaa cgtgaaaaaa tccagcaggc ttcgactgta gctgaaacca atctagctta
     8881 tgtctcagaa gtcatgcctg taggagtgat tgcttatcag ccatcaagcc aaaaaatcga
     8941 atggctcaat ccattagcat tgaaaatcaa agatcaaagt ggattaacag aggaagaatt
     9001 gctagatact ttttttactg caaaagaaaa aaagaaaaac caagtctatc tagcagataa
     9061 aacatttcag tttgatatgg atacagtaaa gggtgttgtt tattttaatg atattaccca
     9121 aaaccagttg ttaaaactcc atcagacgaa cagtcagccg gtgattggta ttttatctat
     9181 tgataactat gcagatgcca ttgataaatt ggatgacaaa gagatttctt atttgaactc
     9241 tttcgtcact acactagttt ctgactggat gaatgaatac catgtctttt tcaaaagaat
     9301 caacgcagaa cggtttttct tcgtagcaca gatgatcgat ctgaaaaaga tgatggataa
     9361 aaaatttgat ttgctggatc gctttcgagc agaagctgaa aaacagagct cgcctatcac
     9421 ccttagtatg gggattgctt atggctcaag aaccctcgat gaaatcggtg gagaagctca
     9481 gaataattta gatgttgctt tagtccgtgg aggcgaccaa gttgtcgtga aagaggtaaa
     9541 agaaggagca aaacctgttt attatggggg gaaatctgct aagaatgtca aacgcacgcg
     9601 tgttcgttct cgagcgatga gtaccgcttt gcgtcggatt tttgatgaaa caggcgatgt
     9661 gtttatcatg gggcacaagt ttccggatat ggatgcttta ggctctgctt ttggtgtcag
     9721 ttgtctggca cgcttcaacc agaaaaaagc ttacatcgtt atcaacgaga acgagatcat
     9781 tccagatgta gaacggtgtc ttgaagaaat ccataagcat cctgaattag aaatgcaact
     9841 aatctctcca gaacgtgcca tagagctgaa aaaagaagag gacctcctcg tgatggtgga
     9901 ttaccataaa ccttctctat cgatctctca gggcctatat gagcaatttg ataagattgt
     9961 gatcattgat caccatcgtc gtggggaaga gtttccaagt aaacctctgc tgacttatat
    10021 cgaatcttca gcttcttcag cttcagagct agtttctgag ctgatccaat accaaagcag
    10081 caaaacaaat cgcttagatc gttttgaggc cacattattg cttgcaggga tggttgtaga
    10141 tacaaatagt ttcaatgtcc gtacaactgc acgaactttt gacgttgcca gctatttaag
    10201 gtcttgtgga gcagacgcat ccttagtaca atatttattg agctcggatt tagcgacata
    10261 cttggagatc agcaatttga ttgctggaag cgactatgtg acttctgata ttgttgtggc
    10321 ggcagggaca gacgaaaaag agtataatag tgttatagca gcaaaaacag cagatacatt
    10381 gctttcgatg atcaatatca atgcagcttt cgtcattaca aagcggacag acggcttgat
    10441 tgggatcagt gccagaagta ccggcacgat caacgtccaa atcatcatgg aatccttagg
    10501 tggcggagga cacttcacaa acgcagctac tcaactggaa aatacgacag ttgaggaaac
    10561 aaaacagaag ctatttgctg caatcaaaga aaatatgaat caaatatatg ataaggagta
    10621 atccaaatga aagtaatttt tttacaagat gtaaaaggaa aaggtaaaaa aggggacatc
    10681 aaagaagttc cagcaggtta tgcacaaaat tatctaatca aaaataactt ggcaaaagaa
    10741 gcaacgaaaa gcagtatcag tcagttagca ggacaaaaga aagcacaaga aaaacacgaa
    10801 gctgaaatct tggaagaagc taaaaagtta aaagaattct tggaaaaaga agatactgta
    10861 gtcgaattga aggcgaaagc aggggaagac agccgtttgt ttggttcgat cccttcaaaa
    10921 caaattgctg aagggttaaa caaacaatac aaaatcaaat tagataaacg aaaaatcgaa
    10981 ttagaaaatc cgatccgttc actaggctac acaaacgtac ctgttaagct tcatcatgag
    11041 gtgacggcta cgatcaaagt tcatgtcgta caagaataac aaatcttgat agcgatgttc
    11101 agctggtaag tataaccaga aggaaaaaag acaaaccgaa atttatctga gaaaacagct
    11161 gcagcaatga aagcaaaagc tgcaaatcga tttcagattg atttcggttt cttttttggg
    11221 aacagcaaaa tagcataaag aaaacgagta taataaaaga gaactatttt tttattacac
    11281 tttcggtacg aatgatgact tttctctcat tttcttgtaa aatgaagcaa ttgaagacaa
    11341 ggagcgatac gtatgaatga agtgtggcag gatcgaatcc caccgcaaaa tattgaagct
    11401 gaacaagcgg ttttaggctc tgtttttctt gacgcagaaa cgatcattga tgccatggag
    11461 tacatagagc caaaagactt ttatcgtcgt ggccatcaaa ttatttttga gacaatgatc
    11521 gagttaaatg atcgtaatga agcgattgac gtggttactg tcaaagaccg gctggaacaa
    11581 gccaatttgt tagaagatgc cggtggttta agttatctct cagacttggc attagcagtt
    11641 ccgacagcag caaatatcgt ctactatgcg aaaatcgttg aagaaaaatc attgctgcgt
    11701 acattgatcc agacagcaac aggaatcgtt acgaaaggat tcgagcaagg agaagaagtt
    11761 caaacaattt tagatgatgc cgaacgcagt atcttggagg tttcggaaaa aagaaatcga
    11821 agcggctttt tatctattgc tgaagtactg aactccacga ttgaaaatat cgatcgacta
    11881 gctcaaaaca atgaagaaat cacaggtcta ccaactggct atcaaggtct ggataaaatg
    11941 acagcaggtt tccagcccga agaactaatc attcttgctg cacgtccagc ggtaggtaaa
    12001 acagcgtttg cattgaatat tgctcaaaat gtaggaacaa aaacagatcg ttctgttgcg
    12061 atatttagtt tggaaatggg ggcagaatct cttgtaaatc ggatgctttg tgcagaaggt
    12121 tcgattgagg ccagccattt acgtacaggt cagctttcgg aagaagaatg gtcgaatctg
    12181 atcgttgcga tggggagtct ttctcgagca aatattttta tcgatgatac accagggatc
    12241 aaaatctctg aaatccgtgc gaaatgtcgg aaactagcgc aagaaaaagg caatctaggc
    12301 ttgatattga tcgattattt gcagttgatt gaaggtaacg gtaaagagaa ccgtcaacaa
    12361 gaagtatcag atatctctcg ccaactgaaa aaactggcga aagagctgaa agtacctgtc
    12421 attgctcttt cacagttatc tcgtggagta gaacagcgac aagacaaacg accagtgcta
    12481 agtgatattc gtgaatccgg atcaattgaa caagatgcgg acatcgtagc cttcttgtat
    12541 cgagatgatt attatgaaag agaaggcgga gatgacgatg atggaaacga gccaaaaaat
    12601 gacaacgtca tcgaagtcat catcgagaag aaccgaagcg gtgcacgagg aacagttgaa
    12661 ctactgttta tcaaagaata caataagttt tcgtcgcttt cgccaagaga agaattttaa
    12721 agaagaggtt gtgagccttg agcaatcccg agagctctcg taagaagatg caaggagtaa
    12781 ccgcaggaac gactagagta cttaatttaa acagatctcc aaagggattg tgacaaaagt
    12841 c
//
LOCUS       DACENJ010000068        12619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.068, whole genome shotgun sequence.
ACCESSION   DACENJ010000068 DACENJ010000000
VERSION     DACENJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.068"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(151..948)
                     /locus_tag="IW748_001368"
     CDS             complement(151..948)
                     /locus_tag="IW748_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1104286.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(945..2129)
                     /locus_tag="IW748_001369"
     CDS             complement(945..2129)
                     /locus_tag="IW748_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1104287.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2154..3401)
                     /locus_tag="IW748_001370"
     CDS             complement(2154..3401)
                     /locus_tag="IW748_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1104288.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3616..5361
                     /gene="dnaX"
                     /locus_tag="IW748_001371"
     CDS             3616..5361
                     /gene="dnaX"
                     /locus_tag="IW748_001371"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1104289.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5381..5695
                     /locus_tag="IW748_001372"
     CDS             5381..5695
                     /locus_tag="IW748_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1104290.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5720..6316
                     /gene="recR"
                     /locus_tag="IW748_001373"
     CDS             5720..6316
                     /gene="recR"
                     /locus_tag="IW748_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1104291.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6380..7024
                     /locus_tag="IW748_001374"
     CDS             6380..7024
                     /locus_tag="IW748_001374"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1104292.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7039..7368
                     /locus_tag="IW748_001375"
     CDS             7039..7368
                     /locus_tag="IW748_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104293.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7382..8320
                     /gene="holB"
                     /locus_tag="IW748_001376"
     CDS             7382..8320
                     /gene="holB"
                     /locus_tag="IW748_001376"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1104294.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8356..9180
                     /locus_tag="IW748_001377"
     CDS             8356..9180
                     /locus_tag="IW748_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1104295.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9173..9520
                     /locus_tag="IW748_001378"
     CDS             9173..9520
                     /locus_tag="IW748_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1104296.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9589..10461
                     /gene="rsmI"
                     /locus_tag="IW748_001379"
     CDS             9589..10461
                     /gene="rsmI"
                     /locus_tag="IW748_001379"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1104297.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10570..11691
                     /gene="dinB"
                     /locus_tag="IW748_001380"
     CDS             10570..11691
                     /gene="dinB"
                     /locus_tag="IW748_001380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1104298.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11745..12347)
                     /gene="nrdG"
                     /locus_tag="IW748_001381"
     CDS             complement(11745..12347)
                     /gene="nrdG"
                     /locus_tag="IW748_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1104299.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
BASE COUNT         4012 a         2366 c         2702 g         3539 t
ORIGIN      
        1 agccaagtaa aaacagtctc ttatgttttt aacttggctg gacttatcac cgtagatgta
       61 agcttttgaa cgccgtttat ttgcaacaaa aggggctgtg acacgatttt tgtcacagcc
      121 ctttttttgc gaacattcat ttagcaattt ttagaaactt ctgccgccac cgccaaaagt
      181 cccgccaccg ctagaatggg tagtacttcc gccaccgccg cttccaggag gtgggctttt
      241 ggggattctt cttgtcgtga caaaagaatt tgtcagacga tcggtttttt cagttaattt
      301 gatactggat ttttctcgga atggatattt gtagatgcct gatttcaatt gataacgcga
      361 aacagtcaca aaataaaaga caagactaag gataagagcc aaaacaacag caatgatgat
      421 ttctactgtc gtcaaaacct tatagcgtgt aattttgcca gtttcttcgt ctacacgata
      481 atgtcctcct ggaactccgt cttccacata tgttgctgat ttagacaggt aagcttttgc
      541 agcattgtaa taggcagcat ttgtcatctg atcataaaca tcatctaaga tcgaatcgat
      601 tctgctatca gtcaaataat cgatcatatt accagaagtc gaaatgtaga tttcacgctg
      661 tcccatatct aatagcaata ctgagccatt ttgatcattt ccgactgcgt ctcttaagta
      721 attatcagca aagtctcttg gttcttctga attagatgta gtcgttacga tcaagactcg
      781 tcctttgatt ttttcattca gcttttctgc ctgttgttcc aaagattgga tttgatctgt
      841 tgtgaataac ccagcctcat catttaccgt agctgtatct gcatgagtag ccaccggcca
      901 gccaacactt actagcaaaa gaaagaataa aaaaaatttt ttcatcatat caagtaacct
      961 ccgatcagaa agaggatcgc tagcccagcg aaaatcccaa gagcggtcag tcccaatttt
     1021 ttctggctga tcggcaggac accgctgacc tttcctgttt gtccattcat tgcgtagtag
     1081 tagacttttt tacttgagtc attgcttcga taagtaacaa gccataccgg cagtaatacg
     1141 taattattta cttcgtttgt gattgaagca gaagttcgca caccggtcaa tgttgtatat
     1201 ccatttgcgg tatcttttaa cagcttctct gaatagtctt ttaattcaga ttgaatctgt
     1261 gatttgattg cctcatattc gatatctctt ttttctgctt ggaacccggc taaatactgg
     1321 cttttaaaat caattgcttt atctaacggg aaaggttgca cgccttcgac catcttttgt
     1381 tgcgtatttt ttgataaggc attttttact agttctttaa aagaaagctt gcctgctctt
     1441 gaaacagcaa attgcttcgt ttctgtgtat tcgatgtcac ctatccgcca tatgcggata
     1501 actgtaccat tcgcctgcat ggcaccatct acttcagcat ctgtagccca ataagggaaa
     1561 tacacacctg tcaacttatc gatttgatcc ttattgaaaa aagctttggg aacaaaccat
     1621 tttttcttgg tccaagcgag aaatctgttg attgcttctt ctttctcgac agcaaaagga
     1681 agtactttat ttggcaagaa ttctccgctt aaacgtcccg atagtacgac gggattatgg
     1741 cagtagtaac aataagtcgc agcagttgtc gcatcagtca cgatctctgc accacagttg
     1801 ggacaaagaa aaagatctat cgctgcggta ttatctgcag attcgtccgc tgtatctgaa
     1861 aaaccgcctg cctcgtcaac cgctttgcgc tcggcttcat tcatatcggc aagctgttct
     1921 tgggctgtaa aagtaaaatc ttcggtaggt gaaggttctt ctttgctttc ttcgccagcc
     1981 atacgcgctt ctttttgctt ttgctcatat tggctgactt cttcttctgt atagatattc
     2041 aggcagtatt cgcaatgaaa tttttggtct ttgggatcaa aagtcaacgg accgccgcaa
     2101 ttaggacatt tatgtgtaag aacatccatc agctgcacac ctttctaaat aaattaatta
     2161 acggggattc ctttaaacgg tttaccacag tttggacaga atttagggat gccgttggaa
     2221 agatccacga tctcattgca ttcactgcat cgcatttgta gcttaggctg aacgttttct
     2281 acaggtttgg cagctccgca gttggaacag aattttccgg aattttctgt cccgcaaact
     2341 ggacatgtcc aagtatccgc atttccttgt gcagtttgtt gattttgttt ttctgcttgt
     2401 tgtttgattt gttcttggtt attttgagct gcttgagaaa gataagaacc atttgcattc
     2461 attcccatac ctacacccat aaagccggtc attgctcctg cttcattttt tccggctgct
     2521 tccataccac gtgcaatgga accttgcacg tatccttcac gtacgctagg atcacctagc
     2581 atcgcccctt cgttacgcat attgatcagt ttcacagaat catcggtgta agaaatgctg
     2641 gctacagcca cagaaacaat ctccatccca cgtaattcac gccaagaatc atccaatgca
     2701 gtatccatgt atttgcttaa ttccaaactc ttagatggta cataagaaat ccgttgacca
     2761 tctgcagaca tctgattgat tgctgattgt agcgctgtta ggaattcagc taaatactgc
     2821 tcattgatat cattgatttc cacttgtgtt ttgtttttag ggattgcgtt tgtataaaat
     2881 aaaatcgggt caacgacatg aatagaataa gtgccatgtg cccgtaagaa taattctgcg
     2941 ttatagaaat tgtcaaagta gttcaacggc gcgcttgtac cgaatttgat tccctttatt
     3001 tcttggagat tgatataaaa aacttgttgt ttctgcggtg tgacgccgcc aaatttgaat
     3061 cggtcaaaag tttcagcaat tgcttctttc agtgttccat tgaacatgga aggagctgcg
     3121 tcatttttaa tggtgtaata accttcctca gcggtataat cgatgatttt tccaccatcg
     3181 accaaaagca tcatcatgtt tggatagaca tggacgactg ttccgtctgt taaaacatct
     3241 tctgttcctt tacgattgga gccacgtttg tcatctttgc ggactttgac accttttgtc
     3301 atgaccgttg tatcactcat attgtctggt tcgatgactt ctagccattg atctgccaga
     3361 ccgccgccaa ccatacttgt tgctgctttg ataagaccca taaaaaattc ctcctctgat
     3421 tcagctagtg ccaaacaatt attgtttatt tattatacca taggaagatg tgaaacttct
     3481 cggactagag acggaattga aatggaaaat tgcgtattgt ttaaatacct gaaaaagccg
     3541 agaaggatag tagttttttt acctaatatc acgtataata agagatagtg aattttttaa
     3601 aaaaggggga tccttatggc atatcaagca ctttatcgag tctggcgttc gcagcgtttc
     3661 gaagacatcg ttggacaaaa agctgtgaca cagacactaa aaaatgctat tgtttcccac
     3721 aaaacctcac atgcctactt gtttacagga cccagaggaa ccggaaagac gagtgcagca
     3781 aagattttcg caaaagcaat caactgtccg aacagtaaag acggtgagcc atgcaacgaa
     3841 tgcgaaatgt gtcgatcaat tactgctggt acgcaagaag atgtgatcga aatcgatgct
     3901 gccagcaata acggagtcga agaaatccga tttatccgtg atcgggcaaa ttatgcgcct
     3961 acaaaggcag cgtacaaagt gtacattatt gatgaggtac atatgctttc aacaggagct
     4021 ttcaatgcct tgttgaaaac attggaagag ccaaaagaaa gtgtcatatt catcttagca
     4081 acgaccgaac ctcacaaaat accagctacg atcatttcac ggactcagcg atttgatttt
     4141 aaacggatca atacggcaga tattgtcgaa catcttgaat ttatcttgaa agaatcgact
     4201 atcagctatg aaccagctgc attgaatgtg attgcccgtt ctgcagaagg cgggatgcgg
     4261 gatgcattga gtattcttga tcaggcaatc tcgtttagtg aaggaacaat tactttagat
     4321 gatgccatgc aagtgacagg tagcttaact gacgaaatga tggacagtta cttgagtgct
     4381 tgcgtgaaaa aagaagtacc agaagcattg gaaacattga accaaattct cgcagccggc
     4441 aaagaatcac gtcgctttct agaggatatg ttacaatact gccgtgactt gctgatgtat
     4501 cagcaagcac ctaacctagt tgaggaacaa tcgagtacga tcagaagtac atttacagaa
     4561 ttggcacaaa ctgtttcagc ggatcagctg ttttcaatga taaagatttt gagcgatact
     4621 caaaatgaaa ttcgttttac caatagtgcc gctatttatt tggaagtcgc tacagtcaaa
     4681 cttgcacagc taccgtctgc ttctgttcct tcagcagatg tagcatttgc agatgatcag
     4741 atagtaaaac agctacaaca agagattaag gaactaagaa acgaaatcaa ggaactacgc
     4801 agtaatacag gaattgagaa tacaaaagta cagccggtac agcaaacaaa tcataaaaaa
     4861 cagacaagta cctatcgtgt accgacggaa cgagtatata aagttctgcg ggaagcgaca
     4921 agacagcatc tgaaccaagt aaaagaagta tgggaagact tgctgatgag tctatctgtc
     4981 actcagcgag caatgcttaa agcaagcgaa ccagttgcag ctggacctac aggactagtg
     5041 attgcgtttg attatgagat tgtttgtcaa agagcagcaa atgacgaaga attgcagctt
     5101 tcggtacaca acaacttaag ccgaatgatc aaagattatg cgcctgatcc tgtttatatt
     5161 acaagagaaa gctggccaga acttcgaaga aattatttga cgcaggcaca agaagaggga
     5221 acagaaaact cttttgcttc tgaagactca gaaggagaaa tcgagttgct tcctcaagaa
     5281 caagaagtac cagaagaaac acttgtagta aaagcagagg aactgtttgg ctccgaagct
     5341 gttacagtgg tagatgatta ataaaaagga gtagataatt atgatgcgtg gaatgggcaa
     5401 tatgcaaggc atgatgaaac aagtacaaaa aatgcaaaaa gaaatggcag aagcacaaga
     5461 gctgctaaat aaaacagagt ttacaggtaa agccaccaat gaattggtca aagcggtttt
     5521 tacaggcgat cgtcgcatga aagatatcca aatcaatgaa gaagtcgttg atcctgatga
     5581 tatcgagatg cttcaggatc ttgtcgtaat ggcagtcaat gatgcgatcg cacaaattga
     5641 tgtggaaact gagaaaaaat tagggaaata cgcaaaaggt ttgccaggat tttagtttta
     5701 aactgaagga gtaatgaata tgcaatatcc agaaccaatt gcgaaactga tcgacagcta
     5761 catgaaactt ccaggaatcg gtcagaaaac agcgacaaga ctcgcctttt atacgatcga
     5821 tatgaaagat gaagtagtaa atgaatttgc taaatctttg ttaagtgtaa aacgcgattt
     5881 gcatttttgt agtatctgcg gaaacatcac agaagaagat ccctgcgaaa tatgtagaaa
     5941 tccttcacgt gatcgaagcg ttatcctagt ggtcgaagag ccgaaagatg taatggcaat
     6001 ggaaaaaatg cgagaatatc acggacttta tcatgttctc catggtgtgt tatcaccgat
     6061 ggaagggaca ggtccagaag acatcaatat ttcttctctg ttgcagcggc tgcatgatgc
     6121 agatgtaaaa gaagtgatta tcgcaacaaa tgcaacaaca gagggagaag caacagctat
     6181 gtatctctca cgtctgatca aacccgcagg tatcaaagtg actcgtctag cacatggtct
     6241 gtctgtaggc tctgatatcg agtatgcaga tgaagttact ttattgaaag ctgtcgaagg
     6301 aagacgtgag ttataaattt ctaattaagt gttttttcag atacaatata aaagcagcac
     6361 aagagatggg ggcaacaatg tgaacggact atttattaca atcgaggggc ctgatggtgc
     6421 aggaaaaacc agcatcttga atgaacttta ccctcttttg aaagaagtag caaaaacaga
     6481 aatcattcaa acaagagaac caggaggcat tccgattgct gaagagatcc gagcagtgat
     6541 ccttgatcca aaaaatgatc ggatggatga acgtacggaa gcactgcttt atgcagcagc
     6601 aagaagacaa catctagtcg aaaaggttct gcctgctctg gcaaaaggga aaatcgtttt
     6661 atgcgataga tttgtcgaca gttctcttgc ttatcaaggt gcaggccgac gcatcggcgt
     6721 taccgagatt gctcggttga atgagtttgc tacagaaggt actacgccgg attttaccct
     6781 ttatcttgac gtagattcag acaccggatt aagaagaatc aagaaaaaca gacaaaatca
     6841 aatcgaccgt ttagattcgg aaggtttgga attccaccag cgtgtacgtc atgcttactt
     6901 aaagctggca gaagagaatc cggaaagaat ccacaaagtt gatgcaagaa aaagctttga
     6961 agaagtgctg caaacaagtt atcatacaat tattgaacag tatccgcaat tttttgaaaa
     7021 ctaggaaggt gaacgtttat gaaaattatt ttagcgatta tccaagacaa agacagcaac
     7081 cgtttatcaa atgaattgat tgatgcaaat atccgtgcca caaaactatc atcgacaggc
     7141 ggcttcctta aggctggaaa cagtacgttt atcgtgggta ttgaagatga ccgtgtagaa
     7201 gaagcattgg aaatcatcaa aaaaacgtgt gaatcaagaa agcaatttgt atctacacct
     7261 gttacactgg atatttcgat ggatgggggc gtcccttatc cagtagaagt agaagtaggc
     7321 ggtgccacag tcttcgtctt acctgttgaa ggattccatc aattttagga ggagctgagc
     7381 tatgaatcaa gaaataaact tggctcaaat gcaaccgatc gtgtatcagc agcttcaacg
     7441 gagttttgag catgggcgta tcgctcatgc ttatcttttt gaaggggaga aaggaacagg
     7501 aaaacatgaa gtaggtatat ggcttgcaca gcacctgttc tgtacccaaa tgaaagacaa
     7561 tctgccttgt ggaaaatgca acaattgtca gcggatcgaa aagaaagaac atccagatgt
     7621 tttggttatt gaaccggaag gacaaaccat caaagtcgat cagatcagac gtttgcagac
     7681 ggaattcagt cgcagcggtt acgaatcaag aaaaaaagtt tttttgatca aagaagctga
     7741 aaaaatgaat agcagtgcgg caaacagttt gttgaaattc ttggaagagc cccctggaga
     7801 ttttttagcg gtgttagaaa cagatgcagt aggaagaatt ctccctacta ttcaatctcg
     7861 ttgtcaagtt ctgcattttc aagaactttc gaaagaagca ctgatccatc gattgcaaga
     7921 agagcagatt ccattggaaa aagcaaaact tttggctttc ttaacaaaca gtttaggaaa
     7981 agcagttgaa atctctcaag atgaatggtt taatgatgct aaggacttga tttatcaatg
     8041 gttcgtttac ttgcaaaagc aagatactca agcctttatc tatgtccaaa aaaaattggt
     8101 aaaatcattt aaagaaaaaa cacaacagtt tacaggatta tctgtcttga tgttttatta
     8161 tcaggaagca cagcgtcaag cagtagcaaa cggcgaatat ggcaaagtga aacagataaa
     8221 ccagataatt gaacggatat tgatcgcaga gcagaaacta cgcagcaatg tcagttttca
     8281 ggctgttgcg gaacagttcg ttttgcatac gattttttag cttgccgcaa aggatgatga
     8341 tcaaggagga tggaaatggt agaagtagta ggtgtccgat atagagatgc aggtcatatc
     8401 tattattttg caccaggaga ctctgaatat gtttatagtg acaaggtatt agtcgaatct
     8461 cagcaatcct tacatattgc tactgttgtc cttcctaaaa aagagattga tcccaatgac
     8521 cttcctgaag ctatccgtcc gatcttgcat aaagcgtcac aagctgaatt agaaaaagac
     8581 aaaaaaaaca aaaaagatgc ttatgatgca ttgaaaatcg caaaagaaaa agtgcagttg
     8641 cgtgagttgg aaatgaaaat ggtcaaagcc gaatacacat ttgatcgaaa taaattgatt
     8701 ttttctttca cagcggacgg gcgaattgac tttagagaat tggtaaaaga tctggccgct
     8761 gtatttcgta ctcgtattga gcttcgacaa attggtgtcc gcgatgaagc aaagataata
     8821 ggcggaattg gtccatgtgg gcgtccatta tgctgttctt cttttttagg cgatttcgtg
     8881 cctgtatcaa tcaaaatggc aaaagatcaa agtttatctt tgaatccaac aaaaatttcc
     8941 ggtttatgcg gacgcttgat gtgctgtctg caatatgaga gcgaagaata cgagacagcg
     9001 aaaaaagaac tccctgactt tggaaaagta attgctacac ctgatggtaa agggaaagtc
     9061 gtagggttga atctgcttag ccgtatcgtt agtattcggc tgactggacg ggaaaatcct
     9121 gtcgaatatg aatgggatga attgaaagaa tttatccaag taggtgagat gaatggataa
     9181 gagatcatta tacgacggat tgaatcaatt agaatcagag ttgagaaata cattaacaga
     9241 acttgttgaa atgaagcatg ctttgcatga gatcgtagaa aaaaatacaa ctttggagat
     9301 ggaaaatcaa cgtctgcggg aacacctgcg ggaattgaac caagttgcac aagctccagc
     9361 tgaaaatgtg aagcaggaac tttctaagtc tcgtatgaat cttgaaaaaa tttatgaaga
     9421 aggattccat gtctgctacg atttatatgg atcaagaagg gacaatgacg agccttgtgc
     9481 gttttgtcta gaagtgatct atcgcgaaag cggtcgatga ccaataacaa gaggagactg
     9541 gagcggagaa atactgctcc agtcttttta ttaaggagat tgtgacagat gtataatcaa
     9601 aaaagtttca agggaaatcc ggtatatggg gatttatatc ttgtaccgac gccaattgga
     9661 aatttagaag atatgagcta cagaagtgtt cgtatgctgc aagaatccga tcttatcgca
     9721 agtgaggata cgcgtaatac acaaaagtta ttgaatcatt ttgaaatcca tacaccccaa
     9781 aaaagtttac atgaacataa ttataaagaa cgtgtccctc aattgattgc tttgttaaaa
     9841 gaaggaaaaa cgattgctca agtcagtgac gcaggaatgc cttccatcag cgatcctgga
     9901 catgagctag ttttagcctg cataaaagaa gggatttctg tgatctctat cccaggacct
     9961 acagctggga tgacggcttt gattgcttca gggctagtgc ctcaaccatt tttattttat
    10021 ggcttccttc cacgtaagaa aaaagaacag aaagaaacat tgaaaaagct aaaagaacag
    10081 acagttacgc tgatttttta tgagtcacct tatcggatca ctgctacatt gacgaatttc
    10141 ttagaaattt ttggaaatcg ccaagtcgtt ttgtgtcggg aattgacaaa ggtccatgaa
    10201 gaatacctta gaggaagtac ggaagagcta ctcgcttata tcgaagaaca tccagtaaag
    10261 ggtgaatgtt gtctgcttgt agaagggaat ttgttcgaaa tgccagtgac ggaagttgat
    10321 gaagagttgg gaacgttaaa ggaacaagta gaagcaaaga tcgcttcagg cgaaaaacca
    10381 aatgctgcta tcaaagcagt agcgctgaaa aatgggctga aaaaacaaga agtctaccgt
    10441 cagtaccatg aattagactg agatagtata agttgtctat aatgaaagac taatgaggaa
    10501 atcagctact aacttttcaa atctcttgaa cgcgaacgaa tgttcttgta taatgaataa
    10561 gaggtgagga tgatggagct gcaatttcct ttacataatg atacatcaag aaaaatcatt
    10621 catattgata tggatgcttt tttcgcttcg gttgaagaac gagatcatcc tgagtttcgc
    10681 ggacatccat tagtaattgc cagacacccc agtgacactg gtgggcgtgg agtagtgact
    10741 acagcaaatt atgaagcaag aaaattcgga attcattcag caatgagtgc acaaaaagcg
    10801 tatgagcttt gtccgcaagc catctttaag cctggcgacc atcataaata cagcgatatt
    10861 tccaaacaag tacgggaagt attctatcgt tatacagata ttgtggaacc agttagtatc
    10921 gatgaagcgt atcttgatgt gaccgaaaat aaaatcaatt gcaagtcagc catcaaaatc
    10981 gcccgaatga tccaagcaga tatttgggag agtgtccagc taacatgttc tgcgggagta
    11041 agttacaata agtttttggc caaattagca tcagactatc aaaaacctag aggaatcacg
    11101 gtagttgccc ctcaagacgc tgtgccattt ttaaaagctt tgccaattga aaaatttcat
    11161 ggggtaggga agaaaactgt acctagaatg catgagctag ggatttatac aggaaaagac
    11221 ctatatgaat gcacagaaat gatgttgatt cgaaattttg gaaaaatggg ctattctctt
    11281 tatcgtaaag tcagaggaat ccatgattct ccagtaaacg ttacaagaga gcgaaaatct
    11341 gtgggaaaag agcatactta tgggcagccg ttgcaaacgg aagaagcagt gctgactcag
    11401 ttgcgccaac tagctgaaaa agtagaagaa gcactgagaa gagtccaaaa acatgggaaa
    11461 acagtagttt tgaaggtcag gtacacggac tattctactg tgaccaaaag agtgacattg
    11521 ccagaatata tctataaaaa agaagcactc ttttatcaag caagccttat atgggaggaa
    11581 atcttgggag tagaaaaagg aatccgattg ttagggatta ctttgacgaa tctggacccg
    11641 atgacttatg aaaacatcgt ccttccatta tgggaaaatc aagaaatata aaaacagctt
    11701 tgagagttga aaccggaaat ccgttttctc tcccaaagct gtttttatat caagtcttgc
    11761 tttttgattt gttcataggt ttctgttgca tccgtacatt tttcccaaat cacaacttga
    11821 ttggattcta aagatttttg gacgtcaatg atccgctggt tgctacttcc gcgaaattgt
    11881 agttttaggt cacgtttgct taattcaaag cgtccgtcaa caaggatatc gatttgcgaa
    11941 aggagttcta atttatcttc tgaatcttgt agcaattcat caaaggtata gcctgtccat
    12001 gaccagatat cttttgtcgt accgaactct tttcggatac gtcgtacgac ttgcagacaa
    12061 acatctgtat tcaaaaaagg ttcgccacca agtaaagtta atccttgcac gtaatcgttg
    12121 cgcaagtcag cgatgatttg ttcttctaat tcaggtgtga atggtttgcc atatcggaaa
    12181 ttttgtgcag ctttgttgaa gcagccttcg caggcaaata agcagccact aacgtaaaga
    12241 ctgttgcgga caccttcacc atcgacaaaa ttaaacggtt tgtagtcagc aatatagttt
    12301 tggctgtagt cttttgctaa ccattctttt ggttttggat ttctcataaa aaaacttctt
    12361 tcatttaagg ttcaaagata tagctattga agataaaatc tactcgacca cataaataag
    12421 aacaaactga cctaaatcca ttgttcaaga aagcttgttc ttattttgat agagggcgtt
    12481 caaagataca aagccatcga aagagggtgg tgaaaaaaga cttgtcatag cctcttattc
    12541 tgaataaacg gtgttcaaaa gcttgcatct gcagtaataa gcccaactaa acgaaaaata
    12601 tgaggaacta tttttgttt
//
LOCUS       DACENJ010000069        12443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.069, whole genome shotgun sequence.
ACCESSION   DACENJ010000069 DACENJ010000000
VERSION     DACENJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.069"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1144
                     /locus_tag="IW748_001382"
     CDS             <1..1144
                     /locus_tag="IW748_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1104300.1"
                     /translation="IYYLLMVIMAVIAQDTLHASLSQAGFASGIYIIGTLLARLFMGK
                     QLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYLVRFLNGFGYGTVSTATNAIVTA
                     YIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMTDFRFIIWFSIVLVFFVTIACLV
                     FPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIAFLMGLSYSSVLSFLSSYTKVIH
                     LVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVMYPSYLFLTAGLFLLSVTTNSVT
                     LLISGALVGLGYGTFMSNGQAVCLKIVREHRISIALSTYFIGLDLGLGVGPYLMGMLK
                     SLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEGELIESLKNEI"
     gene            complement(1292..1657)
                     /locus_tag="IW748_001383"
     CDS             complement(1292..1657)
                     /locus_tag="IW748_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1104301.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(1804..2577)
                     /locus_tag="IW748_001384"
     CDS             complement(1804..2577)
                     /locus_tag="IW748_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1104302.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(2751..3611)
                     /locus_tag="IW748_001385"
     CDS             complement(2751..3611)
                     /locus_tag="IW748_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1104303.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3611..5029)
                     /locus_tag="IW748_001386"
     CDS             complement(3611..5029)
                     /locus_tag="IW748_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1104304.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5086..5535)
                     /locus_tag="IW748_001387"
     CDS             complement(5086..5535)
                     /locus_tag="IW748_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1104305.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(5980..6243)
                     /locus_tag="IW748_001388"
     CDS             complement(5980..6243)
                     /locus_tag="IW748_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1104306.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6342..7637)
                     /locus_tag="IW748_001389"
     CDS             complement(6342..7637)
                     /locus_tag="IW748_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1104307.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(7634..8905)
                     /locus_tag="IW748_001390"
     CDS             complement(7634..8905)
                     /locus_tag="IW748_001390"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1104308.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9070..9939)
                     /locus_tag="IW748_001391"
     CDS             complement(9070..9939)
                     /locus_tag="IW748_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1104309.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(10231..11841)
                     /locus_tag="IW748_001392"
     CDS             complement(10231..11841)
                     /locus_tag="IW748_001392"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1104310.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3477 a         2666 c         2150 g         4150 t
ORIGIN      
        1 gatttattat ctgttgatgg tcatcatggc agtcattgcg caagacacct tacacgccag
       61 cttgagccaa gcaggttttg cttctgggat ctatatcatc ggaacattgc tggcacgctt
      121 attcatgggg aaacagttag aactatttgg tcggaagttt accttaagag gcggcgcgat
      181 cttctatctc ctttcaacat tagcatatct ttatacgcca actatcgggg cattgtattt
      241 agttcgattc ttgaatggat tcggctacgg tacagtttct acagctacaa atgctatcgt
      301 tactgcatat atccctgcat ctaaaaaagg ggaaggcatc aattattatg gattaagtac
      361 tagtttagct gccgctgttg ggccattttt aggaatgatt ttgctgaata tgactgattt
      421 tcgtttcatt atctggtttt caattgtact ggtcttcttt gttacgattg cctgccttgt
      481 ctttcctgta aaaaatattc cattatctga agaacaaaaa atatctcttc gctcatggaa
      541 tatcaatagt tttattgaaa agaaagcact tttcattaca ggaattgcct ttttgatggg
      601 tctttcttat tcaagtgttt tatcattttt atcttcttat acaaaagtaa tccacttagt
      661 tgctgctggg tcatttttct tcgtcgtata tgctttggtc atcacattta cacgtccatt
      721 gactggaagg attttcgatg tcaaaggaga acaatacgtg atgtatccta gctatttatt
      781 cttaacagca ggattatttt tactgagtgt aacgacgaat agtgtgacat tgcttatctc
      841 tggagcatta gtcggcctag gttatggtac gtttatgtca aatggacagg ctgtttgttt
      901 aaaaatcgtc cgcgaacatc gaatcagtat tgctttatct acctacttta ttggtttaga
      961 cctaggctta ggcgttggtc cttatttgat ggggatgcta aaatctctta cttcatttag
     1021 aggattgtat gtgattgccg gagtcattcc attgatatgt acagcactct atcttgttaa
     1081 tggccgaaaa catagtcatg aagcagaggg cgaattgatt gaatcattga aaaatgaaat
     1141 ttgatcttga tgaatagata aaaaaaaccg ttcagatctg aaaataatct gagcggtttt
     1201 ttgagtaaaa atagtttatg attattctta attaaacatc tatttatttt taaagctttc
     1261 atttatcgat aagaatagtt taactgttga tttattccat tttccgttca tctggtacga
     1321 tgccttgact tagcaaccac ttgtggaaga accattctgc cacaccgatc aatagagcag
     1381 gaatcaatac tttcatccaa atatcgactg tatcattcat aaaatataga tagagccaca
     1441 agataactgc agctgaacca gcatctgcga tcgtcgctgc gatgttccct actttacgta
     1501 agatcaacag atcccctagc acatagatca tgactacaat aattacccca acaatcgttg
     1561 aatccataaa gctaacaccg tataaacctg tcagcacgac ccacagaatc gcccagatca
     1621 tgattgcctt cactaccaac gccttcacat gtttcatttt ctgcactcct ttctcctgga
     1681 taaaaaagta agattatcta acaagataac cttttcatta ttataccatc ggctgagaga
     1741 agcgagcaaa gataaggtcc tatctaaaaa tgaggaagcc acttagaaca ttcacgtaat
     1801 aatttatccg tacagacatt cgatatggtg ttcctttact ttcgcatact gttcttttgt
     1861 cagttcttta cttgtcacca gataatcgat ttcatgaaaa gcaatggctt gatgcatgaa
     1921 tttatgtgca attttttgct gtgtaactaa aacggcaagc cggtctgatt gttttctaaa
     1981 agcttttttg accattgcct ctccttcatg agtttctgtt actccttggc tgaaatctag
     2041 cccgcgacaa ctcattatcg ctaaatccgc ataatatgac aatgcatcgt tataggcttt
     2101 tgaaccgttg attgttgctc gtttcggaac aattgcccct cccaatacag atacttttgc
     2161 cgttgtatat tcactcaaca tagcagctgt gacgatgcca ttcgtaatga tctgcaaacc
     2221 atcaaatctt gctaggtttc tgactaagtg taaagaggtt gtactggaat ccaagaataa
     2281 gaccatatca tctcgaatca aagaagaagc tgtatctgca atcttacgtt tttctgtttc
     2341 ttcttctaat ttggtgatct tcatcaggta aggcacgtca atttttcgat tgtcaataag
     2401 catggcgcca ccgcgaactc tacggatcaa tccacttttt tccagaaaaa caaggtcacg
     2461 tttaatcgtg gcttcgctat aatttactaa ctcgatcaaa tcggtgactc ttgcaaattg
     2521 gttgtttttt agataattca caatctcctg ctgtctattc tccacttcat tcaacataaa
     2581 atcaccccat ttactttata tagtaacagg aaaaaagagg ttgtgacaga agtgattggc
     2641 ttcgagaaat aagaagaaat ttttcgaaaa aactacttct gaacatcgtg tgtggttaag
     2701 agggtatgac aagactattg tcataccctc ttgaccacac atttttattt ttagtataaa
     2761 ctagccttgc ctactgtgtt caaaatttcc attttatgtt tgaccacttc ttgtaccttt
     2821 ttattagctg ctggataaat tgcattcggc tcgtacatcg atggattttc actcaatatt
     2881 tctctcaaag cattgaaaaa gacgctttta agatctgagc tcaaattcac tttggcaact
     2941 ccgtatttta ctgcttcgct gacttctgcg tccggattac ctgaaccgcc atgtaaaaca
     3001 aatggaagat cgatttgttg gttcaattct ttcagtaagt cgatatttaa ttttggagtt
     3061 ctgtttttcg gatacaagcc atgcgctgtt ccaattgcta cagcaagtgt atcgattcct
     3121 gtttctttca caaaaatctt cgcttgatca ggatctgtat aaatgattgt ttcagcacct
     3181 ccttcagcag aaccattatt cccaatcgtt ccaagctcag cctccactga gacatttacc
     3241 gcatgagcta acgcaaccac ttttttcgta atctgtacat tgtcgtcgaa tgatttcaaa
     3301 gacgcatcga tcatcacgga agaatagccg tttcgaatcg cccgtaagac atcttctatc
     3361 gtaccgccat gatccaagtg gataacgatc ggcagtttac ttgtataagc ataggctctt
     3421 actgccgcaa caaactcatc acttaaatag gcgatttcat caggatgaat ttctaagatt
     3481 actggtgctt ctaatctctc tgcttcttcg atgattgctt tcatcatatc atagctgcaa
     3541 atattgaagg ctggtactgc aaattgattc tctttagcca ctttcaataa ctctttcatt
     3601 gtatataaca ttacgcttcc acctcatttg ttagattttc atttttcgtc cacttgtctt
     3661 gttttttctc tttcactgtt tttttcaata gtccaagcat aaaggctgta gtgactgtac
     3721 ctgatgcaat tgccaagaca tacataggta aattgttcat aacatttgga attagcatca
     3781 cccaaatccc tccatgaggt gccattgaag taactcctac tgccatggaa atccctgcag
     3841 cgactgctga acctgtcatc aaactaggta tcacgcggat cgggtcagat accgcaaacg
     3901 gtatagcacc ttctgtaata tacgaagcgc ctaaaatcca gcatgattga cctgaagtcc
     3961 gttcttcttt ggtaaattta tgtttgaata ataaagtggc taaagcaata cctaggggag
     4021 gtgtcattcc tgcagccata caagcagcaa tcggtgcatt tactccagct gacatcaaac
     4081 cgatcgagaa agtcgaagtc gatttattga tcggcccacc catatcagct gccatcatga
     4141 tcccgataag taatccgaac aatagcccgg atgtatttcc caagctattc agccaatttg
     4201 tcaaagcatc caacacccat gtgatcggtt gttcaatcac aaataccatc aaaagcccaa
     4261 taattaagat cgataaaaag gggacgacaa tcagctgata aatagcagaa aattcttttt
     4321 ttacgctaat tttatttgcc agaaaatcac agatataccc tgctagcacg ccaccaatca
     4381 ttccgccaag aaaacctgaa ccacctgtag aggccagtaa tccggcaaca attcctgggg
     4441 caaaacctgc ttttccagca atcgatgtag caatccccgc gcctaacgca ggaaccatca
     4501 tggcaaacgc tgtatctcct ccgatccttg aaaatgcagc tgctaacaca ttatagtctg
     4561 ctgagttggg atcagccgct gtaatcccaa aggcaaaact cagtgcgatg atgattccgc
     4621 ctgcgatcac aaatggaagc atgtaattca caccattcat gaaatgatta tagattgtcg
     4681 gctttttctc atttgcagct ggttcttttt cattagacac aacgaatacg ccaatatctc
     4741 tcaatgcttc gtctattact acttgcggtt ctttgattgc tcggcttgta gagacacgct
     4801 ttactttttt cccggcaaat cggtccaatt cgatttcttt atctactgca agaatgattg
     4861 catctgcctc tttgatttct tgttcagata actgattttc tgtttttaca ccttgtgttt
     4921 cgaattttgc ttgatggttt agatttcttg ctgttacttc taatttttct gccgacatat
     4981 aagtatgagc aattccagtc gggcaagcag ttacaccaac gattttcatg attattttcc
     5041 tcctttttct tcactatcga tactcggaca aagactctac tattttcatt cttgactaat
     5101 ctgtctgatg atatctgctg gcgtagtggc ttcttttatt ccctttctaa aatctgcatt
     5161 catcaatcgt ctggagagtg tgctgagaat ctctagatgc atatctgctg atttttcagg
     5221 aactacgatg gcaaaaatat attcaattgg catttcatct atcccgctat agtccgttaa
     5281 tttggctgta tgaaaaaaac acagtgctgg ttttgtcaca tatttgcttt ttgcatgggg
     5341 aatcccaaat ccatcttcta ttcctgtgga gatctcctcc tctcgtttga atagtgcctt
     5401 aacgactttt tgaacatttc ttaccttatt tgtttttttc aacacatccc ctattcgctc
     5461 aaataatgat ccttttgaat ctatcgatgg atcgaaaaca atcagttctt ctgcgatcaa
     5521 ttgatcaaac ctcataaaaa acctctcccc cacaaaaaat gttaacgttt tcttggaact
     5581 gaggtcagta tagcatcgct agctgtaccg ttcaatatta tgaatgaaca gaggaaaaag
     5641 acccatcatc cgcgtgtata agtcattaaa atgaacgatc tagctcacaa acaatcgcaa
     5701 gtctataaca aaacaaaaag ccttccttga aaggaaagct tagcttctaa ttttaggtaa
     5761 atggtttttt acttcgaaat aacccctctt catttcgtta atataaggtg tttttatcaa
     5821 gtggataatt aaaactatat actagttttt cattgttttg tgcattatga tcctacgact
     5881 ttttcagcat caaacgaagt tttaaattgg caaaaaacat aaaaaaacag actatcccta
     5941 tcagatagtc tgctaaacgt tgagttatca gcgtttttat tattccgcag cgtttgcgtc
     6001 tttgagaccg tattttttgt tgaaacggtc cacacgtccg tctgcttgtg tgaatttttg
     6061 acgtccagtg tagaatggat gtgagtcaga agttacttcg acacggatga ctgggtatgt
     6121 attaccgtct tcccattcaa ctgtttcttg tgaagttttt gttgaacctg acaagaattt
     6181 gaaaccagtt gttgaatcca tgaatactac tgggtggtaa tctggatgga tgttttcttt
     6241 catgattttt tcgctccttt gccctgctcc atttgctgaa ccagagttga tttgtcgatt
     6301 tacaacatta acatcttaac atgtttcagt caaggttgca attatctttt cagatttctg
     6361 gtcggatttt ttttaccaaa ggagacatct tgaaattctt tgaaaaattg ctgattgttt
     6421 tttgttttac gcaaaaagcg gatcagttgt tccgtatatt ctaatgaatc tccagtcata
     6481 ttttttcgaa gtttccaaat ttcttctaat tcttcacttg gcatcaataa ttcttctttt
     6541 cgggtacctg attttttgat atcgatggct gggaagatcc gacgttcaga taattcacgt
     6601 gaaagttgta attctagatt tccagttcct ttgaattctt catagatcac gtcatccata
     6661 cggcttcctg tatcgactaa agcggtcgct aaaatcgtca aactgccacc ttcttcgatg
     6721 tttcttgctg ccccgaaaaa tttcttaggt ttatacaatg cggctgggtc aatcccgcca
     6781 ctcaatgtac gtccgcttgg tggaacgact aagttatagg cccgagcaag tctagtaata
     6841 ctatccatca agataacgac atctcgttta tcttcgacta aacgcattgc gcgttccaat
     6901 actaattcag aaactcgagt atggttttgc ggctgcagat caaatgtaga agataccacg
     6961 tcgcctttca cgcttcgttc tagatccgtt acttcttccg gtcgttcatc aatcagtaga
     7021 acgattaatt ctacttccgg atagttttcg gtgataccat ttgcgatttc ttttaataca
     7081 gaagttttcc ctgcttttgg cggtgcgacg atcaatccac gctgaccaaa accgattgga
     7141 gaaaagatat cgatcattcg ggtagatagt ctttgcggtg ttgtttccag ttgaagctgt
     7201 ctttgcggat aaagaggtgt taaggctgga aaatgcggac gttcttttgc ttcttccgga
     7261 tcttttccat taacgctttc cacgtgcatc aaaccataat aacgttccga ttctttaggt
     7321 ggacgggctt ttcctgccac tttgtcccca tttctcaaac tgaatcgtct gatttgtgag
     7381 gcagaaatat agatatcttc tgcacttggt ccataattga ttggccgtaa aaaaccgtaa
     7441 ccatcttgac caacgatatc caatattcct tccatataat aaaatccttg tttctctgct
     7501 tgtgcacgaa tcacagctag agataattcc tttttattca tcttactgta ataaggaatt
     7561 ttaaattctt tggcataact ataaacttct tttaacgttt tgttctctag ttctgccatt
     7621 gtcaaataat cgctcattca gctcccactt cctctatcat ttcaattgat gcccctaagg
     7681 ctgttagttt ctcgataata tgatcataac cgcgtaagat ataatctaca ttgtaaatcg
     7741 tcgttgtccc ttctgccaat agtccagcaa tcactaaaca agcacctgcc cgaagatcag
     7801 aagccacgac ttctgtgccg tgaagtttat ttggaccatt cacgatgatc atatttcctt
     7861 ccacggtaat atcagcgccc attcgtgcta gttccggcac atggttcaca cgtttcgcat
     7921 agatcgtatc gatgatttct gaagtgcctg tagtcatcaa tagtaacgca gtaagtggct
     7981 gctgcagatc tgtggcaaat cctggataag gataggtcat gatgtttgcg ggttttaatt
     8041 ctttcgaggg gtaaacttcg atttcatctt cgctgatttt catatggaca ccaatttcct
     8101 gcaattttgc tataaagctt tccaaatgtt caaagatcac atttttcact ttgataccat
     8161 ttcctactgc agctgctaat gctagatagg ttcctgcttc gatgcggtca ggaatcatga
     8221 aatgccgaca gccatgtaat gtttcgactc cttcgatacg gatcacatct gtacccgctc
     8281 ctcgaacttt tgcccccata ttattcagta aagttgccac atctatgatt tccggttctc
     8341 gtgcagcgtt ttcaattact gtctgtcctt ttgcttttac agcagctaac atcacattga
     8401 tcgttgctcc gactgaaacc acgtccatga aaatccgatt gccacgcaat cctttatttt
     8461 ctgtccgtaa atacatggca ccatgttcat ttgtcacttc agcacctaga gcttcaaatc
     8521 ctttgacgtg taggtcaatc ggtctagggc ctaaataaca gccacctggc aggcctacta
     8581 ccgcttcgcc aaatttacca agcagtgttc ccataaaata ataagaagca cgcaaactat
     8641 tgatttttcc gtgcggcatc ggaatcgata cgacttctcg aggatcgatt tctagtatgt
     8701 tgttggaaaa atggacagtt gcgcccatga tctctaaaat ttcaattaat gaatgaacat
     8761 cttgtatgtc cggcacacct tccaagatca ctggggaatc agctaagatt gcagctggaa
     8821 tcaatgcgac tgcactattt tttgcaccgc tgatcgttac ttcaccagct aaaggtcggt
     8881 ttcctttgat tacgattttt ttcatctgtt tccaccttgt ttattaatct tttttcatgg
     8941 atatgcttgt attttatcat agataaaaaa aagaaacgag catggtctgc cgttttaagt
     9001 aaaaaagaat ggagagcgac ttcaaaagtc gttctccatt cctatatatc gtatctgtta
     9061 tgtggatgct tacgctttgt tagctgaacc gaaccagtcg atatgagatt tagcatcttc
     9121 aacgattgct gctttacctg gagctaataa tttacgaggg tcgaatcctt taccttcttt
     9181 atctttacca gcttcgatat atttacgagt agctgcagca aatgataatt ggaattcagt
     9241 gttaacattt acttttgaga tacccattga gatagctttt tcgatttgat cttgaggaat
     9301 acctgatccg ccatgtaata ctaaaggcat gtctggacct acagcatcag caattgcttg
     9361 taagtggtca aaagctaatc ctttccagtt ttctggatat tgaccatgga tgttaccgat
     9421 accgcaagct aagtagtcga tacctgtagc aaccatttgt ttgcattctt caatatcagc
     9481 taattcgccg ttaccgatga ttccgtcttc ttctccgccg attgaaccaa cttcacattc
     9541 aactgagata ccttttgcat gtgctttttc aacaacgtct ttagctaatt tcaagttttc
     9601 ttcaaatggt agatgtgaac catcgaacat tactgaagta tagcctaatt cgatacattc
     9661 caaagcagct tcgtaatcac catggtctaa gtggatagca actggaacag tgatgttcat
     9721 tgcttcaact aaatcagtga ttaaatcttt acagattttg taaccaccca tgtatttagc
     9781 tgcacccatt gatgtttgga taagtactgg tgcttgtttt gcttcagccg cttcaaggat
     9841 tgcttgagtc cattccaagt tgtttgtgtt gaatccgccg acagcgtaac cgcctttacg
     9901 agcagctttt aaaaattcag ctcctgatac tactggcata ataaaattcc tcctaagttt
     9961 tgtaaaacta aatttgttaa gacaattctt aaccaacgac cctattttac caaacatttc
    10021 ggtagttttc tactaaaatc aatgaaaatg aaaattttca agaagttttt tgcaaaaaat
    10081 aaaatatcca atagcgcttt aatcgaaaaa agtcttttgc tttctatctt ttttcctaca
    10141 aataataaca aatcagaaaa agagaaaagt gacaagaaaa ttgcttatcc ccttctcttg
    10201 ctacttttct ctttctatca agttcaaaaa ttattttgtt tcgtgatctg ctaatgatgc
    10261 tccgacaaat gctttgatta atttttgcgg gcgatttgga cgagaaatca attctgggtg
    10321 gaattggcat cccacaaaga attttttctc tggcagctct acgatttcta ctaaacggtt
    10381 atctggtgag acacctgaaa agactagccc ttgttcttca aacaattgac gatatttgtt
    10441 attgaactca tagcggtgac ggtgacgttc ttgcacaaca tcggctccat catatgcttc
    10501 agccgtcttc gttccttttt tgattttaca tgggtataat ccaaggcgta aagttccacc
    10561 caaattctcg atgttttcct gatcagccat taaatcaata atattgtttg ctgtatctgg
    10621 attcgtttca gctgatgacg catcttctaa tccaacgacg ttacgtgcaa attcgacaca
    10681 agccatctgc ataccaagac aaatacctaa aaacggaacg tcattttcgc gagcataacg
    10741 aatcgcttcg attttccctt ctactccgcg atcaccaaat ccaccaggaa ccaagattcc
    10801 atctgcgtct ttcaaacgtt cttctacatt ttctctcgtt aattcttgtg ctttgatcca
    10861 atcgatctcg atgtctgaat tataggcgaa accagaatgt ttcaatgctt caactactga
    10921 gatatatgca tctggtaact cgacatattt accgacaagc gcaatccgta cttttttctt
    10981 caagttcaag acacgttctt ctaattcttt ccattctgtc atgtctgctt ctggtgtatc
    11041 tagttttaaa tggtcgcaaa caatttgatc cattccttgt gcttgtaaaa ctaatgggat
    11101 ggagtaaagt gtatctacat ctggcgactc gataactgct tctggttcga catcacaaaa
    11161 ttgtgccaat ttgtttttca cgctttgaga aacaggttgc tctgtacgta ccaccaaaat
    11221 attcggttgg atacctaaac cacgtagctc ttttacactg tgctgcgttg gtttagtttt
    11281 catttctcca gcagctttca agtaaggaat caatgttgta tggatataca taacattatc
    11341 tgtaccgaca tctgctttca tttgacgaag tgcttcaagg aatggcaaag attctatatc
    11401 ccctactgtt ccccctactt ctgtaatgat cacatctgaa tcagtcattt tagctgctcg
    11461 catgattttt tctttgatct catttgtaat atgaggaata acttgaacag ttgctcccaa
    11521 gtattcgccc ttacgttctt tacgcaaaac ttcagaatag atttttcctg tcgtcacgtt
    11581 ggagtatttg ttcaaattga tgtcaataaa acgttcgtag tggcccagat ccaaatctgt
    11641 ttcagcacca tcatctgtta cgaatacttc tccgtgctga tatgggctca tcgtccccgg
    11701 atccacgttg atgtatggat caaatttttg gatagttact tttaagccgc ggtttttcaa
    11761 taagcgtcct aaagaagctg caacaatccc ttttccgatt gatgaaacga caccgccggt
    11821 cacaaaaata tattttgtca taagttcaaa aactcctttt taatttatgt gcaggaaaag
    11881 tctcagacaa taaacaaagc tccctattct tcaataagaa tagggagccg aaatttctgt
    11941 aaacttctga ccctctaaaa gggtgcccaa ggtgtataat acaagctgac taagggaaag
    12001 tcaagtactc aaatgaatac tatccgctta caattttttc tttttcgata tcctttgaga
    12061 aattatttct taagtccttt atgagtgcaa aaaaaattta acccacataa taagcttgat
    12121 ataaacatag ctaacgttaa acctaagcaa atcagaaaat cgttactgaa aaattttaag
    12181 tgaaataata gataaaaaaa taaaacaaac attatgatag ataatatccc acctaacaaa
    12241 gaactacttt ttcctacttg cagcaacagt ttatatagct tatcgtagaa acaatctttt
    12301 tctttcaccc ttatttacct cctttacgta aaaaacgtag tccacagttc tcggattttt
    12361 ggcatatctt ttctattaaa taatatcaaa aacaaaacaa ttccactaat tttttcaaag
    12421 atgacctttt tttcaacaaa agt
//
LOCUS       DACENJ010000070        12116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.070, whole genome shotgun sequence.
ACCESSION   DACENJ010000070 DACENJ010000000
VERSION     DACENJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.070"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1001
                     /locus_tag="IW748_001393"
     CDS             <1..1001
                     /locus_tag="IW748_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1104311.1"
                     /translation="FRVRFPTTDALAIIRDVLQLKSNYMLYEDYGKYGYESKYVLGDI
                     NIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMTAGGVMKRLDLAIND
                     KAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTGETLYLGSTSSELYM
                     CAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLTYRDAERTAFSIINH
                     YVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTLQKALHWLQRQVAPT
                     IKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERIDTAVPQENDGIF"
     gene            complement(1711..1899)
                     /locus_tag="IW748_001394"
     CDS             complement(1711..1899)
                     /locus_tag="IW748_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104312.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            1990..2199
                     /locus_tag="IW748_001395"
     CDS             1990..2199
                     /locus_tag="IW748_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1104313.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2311..3924
                     /locus_tag="IW748_001396"
     CDS             2311..3924
                     /locus_tag="IW748_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1104314.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            3959..5170
                     /locus_tag="IW748_001397"
                     /pseudo
     CDS             3959..5170
                     /locus_tag="IW748_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5193..6119
                     /gene="glsA"
                     /locus_tag="IW748_001398"
     CDS             5193..6119
                     /gene="glsA"
                     /locus_tag="IW748_001398"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1104315.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6327..7787
                     /locus_tag="IW748_001399"
     CDS             6327..7787
                     /locus_tag="IW748_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104316.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            8008..9771
                     /locus_tag="IW748_001400"
     CDS             8008..9771
                     /locus_tag="IW748_001400"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1104317.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9855..10313
                     /locus_tag="IW748_001401"
     CDS             9855..10313
                     /locus_tag="IW748_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1104318.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10422..11105
                     /locus_tag="IW748_001402"
     CDS             10422..11105
                     /locus_tag="IW748_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1104319.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            11252..11866
                     /locus_tag="IW748_001403"
     CDS             11252..11866
                     /locus_tag="IW748_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1104320.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11882..12088
                     /locus_tag="IW748_001404"
     CDS             11882..12088
                     /locus_tag="IW748_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104321.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         4185 a         1957 c         2559 g         3415 t
ORIGIN      
        1 actttcgtgt ccgctttcct actacggacg cattggcgat tatccgtgat gtattacaac
       61 tgaaatctaa ttacatgctt tatgaagatt atggaaaata cggatatgaa agcaaatatg
      121 tacttggcga catcaatatc atgtgttcca tgcaggagca tttaggtgtt ttattggaac
      181 tgaaaggcaa aggctgtcgg caaatggaat gttatctgct ggcacaggaa cgctcatggt
      241 atgacttcat gctggattgt atgacggctg gtggtgtgat gaaacggctt gatttggcta
      301 tcaatgataa agcaggaata ttggatattc caaagttaaa ggaaaaatac aaggctggcg
      361 aatgtatgtc ctacttccgt atgcagaaag attacagcgg tacagaaaaa tgcggtagcg
      421 atttaccaaa gaatacagga gaaaccttat atctcggttc aacaagtagc gaattatata
      481 tgtgtgctta tcagaaaaat tatgagcagt atgtcaagaa tggcacagaa attgaagata
      541 cggagattaa gaaccgtttt gaaatacgca tgaagaacga acgagcctat tatgcggttg
      601 tagatttact gacctatcgg gacgctgaac gcaccgcttt ttctatcatc aatcattatg
      661 tccgctttgt tgacagagag gacgacaagc caaaaagtca atggataaca aatgatgatt
      721 gggcatggtt tgtaggggaa aacagagagc cgatacgctt aaccacaaag ccagagcctt
      781 acactttaca aaaagcgttg cattggctac aaagacaagt cgcaccaacc ataaaaatgg
      841 tacaggcatt agacagagaa aatcgtacaa cgatactgaa agatatgatt gagcaggcag
      901 aactaaaaga caagcataaa catttattac aattagagaa atcaaccata gaagaacgta
      961 tagataccgc tgttccacaa gagaatgacg gtatttttta atgtaattga aaaatttcag
     1021 atttagtcag caaagagctg ttttcattcc ctatgtgaag tgaaagaaga aaaataaatt
     1081 ttttattaaa tctgcaacaa aatggctact tgcgtacaga tatggtgcga gaaaaggaaa
     1141 tctaaacttt ttttgaaaat aggtcattaa atcggggctt gctgtcccta tatgaagtga
     1201 aagaagaaaa aagttttgaa aaaatgacgg aaattcgtga ttagtggaca gtcttctgtg
     1261 aaaagaggaa aaacatcatc attttgtatt caaacaggca ttagcagttg agtgttgatg
     1321 tgaaaagatg aaaatacttt gttctcaact taacaattta aggtttccgt tccctatatg
     1381 gtgcaaagaa aatatttcat tctattgttg gcagtaacgg tatggtttgg gtagttaggg
     1441 tgtgaaaagg aaaatattct tttttcatca tcaacttagc aaaatgacat tttctatccc
     1501 tatatggtgc atgaaaagaa gttaaaattt ttccatttaa acttagcaaa tggacgtttg
     1561 ctgtacagat agtagtgaaa aaggagaaaa tcaaccgttg attgccaaaa tgaaccgttc
     1621 catttgtact aataatggaa aaagttttgg attttggtta cgtttctcct cttttccaac
     1681 gcgatatata ggaaagacga tttttttctt tcaatcgttc tttgacaact gaataaagct
     1741 attcaatacg tttgacagac agctagccgt tgaaacaaat acgccacgac ctttcccctg
     1801 caagagcgag cgaaaaccta tttgtgttct tgtaaaagat ttgtcctctt ttatcgccat
     1861 gacctgtact gtcagataat gatactcccg tacagccaca gcttgagcat taagagcgtc
     1921 gcacgcaatg ggtacggcta cataagaact atgcagaggt ggaactcctg tgggctatga
     1981 caaaagccgt tgaattgctt agaaagattt tacagaaagg gggtatgtgt tattgataat
     2041 gctgtaaaaa caattcaaaa gaaaaatggc aagtgtggca ttggcaacag aggaaagaca
     2101 aagattgtag taaaagagca tttttccgaa aagggcaaaa caatggaaga acttctaact
     2161 gatgtcatgc tggaaaaagc aaagcagaca atcgcataaa atcggtgcag ttgtaagaaa
     2221 tagattgaat gacaaaaagt acccgtgtta tactttactt gcaagcaggt attgtaaaca
     2281 cgggttagtt ataaaggagg ataactgaca atgaaacaac agatttacaa tactgcactt
     2341 tatcttaggt taagccgaga tgatgaatta cagggcgaaa gttccagcat taccacacag
     2401 agaagtatgt tgcgtttata tgcaaaagaa catcatttga atgtcattga tgaatatatt
     2461 gatgacggct ggtcgggaac aaattttgac agaccgagtt ttcaaagaat gattgaggat
     2521 atagaggcag gaaaaatcaa ctgtgttgta acgaaagacc tttcccgtct tggcagaaat
     2581 tatattatga caggacaata tacagaattg tatttcccta gtcataatgt ccgctacata
     2641 gcgattgatg acggtgtaga cagcgaaaaa ggcgaaagtg agattgcacc atttaagaac
     2701 atcatcaatg aatgggtggc aagagataca agccgtaaag tcaaatcagc atttaagaca
     2761 aagtttgcgg agggggctca ttacggtgct tatgctccgt taggatataa gaaacaccct
     2821 gacatcaaag gaaaactgtt ggttgatgat gaaacaaaat ggattattga aaaaatcttt
     2881 tccctagcct atcaaggtta cggaagtgca aaaatcacaa agcagttaag agcagaaaaa
     2941 gttccgacag cgtcatggct aaattttaca agatacggta cttttgcaca tatctttgag
     3001 ggaaaacccg aaagtaagcg ttatgagtgg acgattgctc atgtcaaggc gatattgaaa
     3061 agtgaggttt atatcggaaa cagtgttcac aatatgcagt ctacggtatc gttcaagagc
     3121 aaaaagaaag tgcgtaagcc cgaaagtgaa tggtttcgag tagaaaacac tcacgagccg
     3181 attattgaca aggaagtgtt ctatcgtgtg caggagcaga taaaatcaag acgcagacag
     3241 acaaaggaaa aggcaacgcc gatttttgca gggcttgtca agtgtgcgga ttgtggttgg
     3301 tctatgagat ttggaacgaa taagacaaat aaaacgcctt acagttatta tgcttgcagt
     3361 tactacgggc agtttggcaa aggtaattgt tctatgcact acattcgtta tgatgtgctg
     3421 tatcaagccg tattggaacg cttgcagtat tgggctaagg cagtacagca ggacgaagaa
     3481 aaggtattga acaagataca gaaagctggc aatgcagaga gaatacggga aaagaagaaa
     3541 aaggcaagta cattgaagaa agccgagaac agacaaaatg agattgaccg tttatttgcg
     3601 aaaatgtatg aagatagagc ctgtgagaag ataacagagc gaaattttgt gatgttgtcc
     3661 agcaagtacc aaaaagaaca gatagaactg gaacagcaga taacaagcct aagagaagaa
     3721 ctaagtaaaa tggaacagga tatgataggt gctgaaaagt ggattgagtt aatcaaggaa
     3781 tattccgtac caaaggaact gacagcacca ttattaaatg ccatgataga aaaaatcctc
     3841 atacatgaag caacaacgaa tgaggataac gaaagaatac aggaaattga gatatattac
     3901 cgatttattg gaagagttga gtaatcaaca agagtaatat ttttaactaa gggaaaccgg
     3961 cggtgtctat acttgggtca gtcgcacatt gggaaaacga ttcggttttg ccgcaatctt
     4021 tttccaatgg tttgagataa cagttggcta cttgaccatg ctgtatttct taactggtgc
     4081 attatcctat gcaacgggta ttggtgctat acaaaacaat aaatatctga aattggcaat
     4141 cctattgatt attttctggg ctattttact ctcacagtta aaaggaacca aatacacttc
     4201 attgattgct cggatcggtt ttgtagctgg tatcttgttg ccagcattag tcttgtttgg
     4261 cttagggatc cattatgtcg cttcaggcgc tccactacaa acgatactgt ctatgaaaac
     4321 attgattcct gatttttcga aattgcctac gctggttgtt tttgtttcct ttattttagc
     4381 atatatgggc gtagaaacat ctgccagtca tgcaaatgaa atggaaaacc ctaaaaagga
     4441 ttatccatta gcaatgtttg tattggttat tctggcaatc attttagata cttttggcgg
     4501 attgaccgtc gctactacag tacctcaaca tggtttgtct cttaatacag gggtgattca
     4561 atcgttagaa ttcctattaa gacacattga tccaaatctt gcatggattg cgaaaattct
     4621 tgcgattctt gtttgtttag gtgtcatcgg agaaattgct tcttgggtaa cctctccttc
     4681 aaaagcactt cacgtagcag cagaagaagg attacttcct gaatattttg caaaagaaaa
     4741 cacacacggt gttcctgctc atttgatgat cgcaaatggg atcgttgcaa caatctgggc
     4801 agccgtcttt acattaagcg gcggggggaa taatctttca tttcttgcag cgatgtcatt
     4861 gactgttgta atctacctga tgttgtactt tatgtttttc gcgggctatt tgaagctggt
     4921 ttttaaagat gacagtcttg aaagagccta tcaggttcca ggcggaaaag ttgtaaaagt
     4981 aatcgttgct tgtttaggtc ttttgacttc actttttgct ttttgtatct catttgttcc
     5041 acctgctagt atcgcagctg gacagcatgg gatttatgaa acgattctga ttgtttctta
     5101 tcttatcgtc ttagttattc catttacgat ctacgcatgc cgtcataaat tccagcatgc
     5161 agaaaaataa aaaatgaaag ctggttattc aaatggaagt aaaagaatta gaacaattga
     5221 ttggaaaaaa tttccactat tatcaaacag gacaagtggc ggattacatt ccagcattag
     5281 ctgaagtgga tcccaaacaa ttaggtatgg cgatttatga tttggataca aaacaactga
     5341 ttgagggagg agacgccgat gtgcgctttg ccatcgaaag catgtcaaaa gttcccgtat
     5401 tgttattagc aatcaaagac aacggaatag aaaaagtctt tcaaacaatc aacacggaac
     5461 caaccggttt tgccttcaat tctatcatga atatggaaat caatcatcgc aaacatccaa
     5521 tgaatccttt tgttaatgca ggagcgattg caacaacctc gttgattgcc ggtaaagatg
     5581 cagatgaaaa atttgagcga atcttgtcat ttatgaaaga gatctgtgat gatcctaata
     5641 tcacattgaa tgaagaaatc tatcattctg aatcaagaac aggtgatatc aatcgctctc
     5701 ttgcttacta tatgaaaggt aatcaaatga tcgaaggaga cgtaccggag atactcgatg
     5761 cttatttcag acaatgctcc ctcaatgtca ccgctacagg catagcaaaa ttagctgctg
     5821 tgctggcaaa taaaggaatt gctccttgga atggcaaaca aatcattact gaagaaagtg
     5881 ctacaatcgt caaatcaatt atgacaactg ctggtttgta tgatgaatca ggagagtttt
     5941 ccgtccatgt tggtgtacct gcaaaaagcg gtgtaggcgg cgggttgatg gcagctgtgc
     6001 caaatcgtta cggcataggt gtcttcagcc cggcactaga tccatttggt aacagcgcag
     6061 ctggtatcca attgttgaaa gatgtagtca aagaactaga cgctgacatc tttgaataac
     6121 cattcttgaa agaggctggg gtaaaagtcc aaacggctca aagctaccta caatgaggct
     6181 gaaattgtga gcttgtttga cttttgtccc agtttctttt tttattggtt tttgaaaggg
     6241 cttttctaaa taaaaaggaa acaaaaatac gatttacaga tatgattaaa taaaatcata
     6301 agaaaaactg gaggcgtgta tcaggaatgc tggaaaatta catcgagcga aatatattta
     6361 gaaaagtgta tctgtgtgaa caattatttg aattccaaga aatagacatc gaacaaacag
     6421 ctatttctct aagggtgact acaccaacga tccttcacga tctagaaagt ctagccgagt
     6481 gtctagaata ttgtataaaa gaacaagtaa gagagaagca taagtataaa ctcgtattca
     6541 aacatggtat tgctctttct gagctcacac agtttctgta cggtcaatct tattttttaa
     6601 agtttttaag ttattatttt aatggtatat tcacgagtac agaactggct gatcttgaat
     6661 ttatttcttt gagtaaagta tatacaatca aaaaaatcgt attggatttt tttaaagaaa
     6721 atgattattt gaaaaataaa gaaatcatta tcccagaatt tgactcgcgg aacatcttgc
     6781 tagctttagt acgttatgtc aattgggaag gctatgaaaa taaaaatccg tatgtagaag
     6841 aagcttgtaa cgagttgatt gattatgtag agactcattt ttttaaaaga agatattcgg
     6901 aagatgaaaa gtcctttatc atacgagaaa tagagatcgc aatcggaaga aaggcatttc
     6961 cccttggact gaaagaagaa gataagaaaa gcgcagaaac gaagccgtta tttcaattag
     7021 tctgtcaagg attggaagag caaaaaggaa ttctagactt acaagaagaa gatgtctatt
     7081 atattttttc tttgttcaac agtagaaatt ataccaatga gaacatggag ctgttaagaa
     7141 aagatttcga agttgtttat aaaaattttg ttctcaatga cctatcactg aatgaattga
     7201 ttttacagat tattagtatc gctgaaatcg acgatgcaga aaattttatt ttcaagcaat
     7261 cctttttaca atttgtccgt acgttgtggg cagatggaca agtattttta ccggaaagga
     7321 tctatcttct atcagaaaaa gaacaacttc tgtacggaaa aattgtagaa atattggaag
     7381 agtggaaaaa gaaaaatcaa atccaactaa gatggaatga aaatttcatt cgaaaattcg
     7441 tcaaaagtct ttctttgatc aacagtgaac aagcggggag acaggaaata gaaatttttg
     7501 ttgtttcaga aagttcagtc aaacaatttt tttatcgaac ccaatttcat ttgtatgcgg
     7561 aagaaggcat cgctgaaata aatcctattt tatttcgttc attagaagaa ttgcctgatg
     7621 aatgtctatg tgcgaaaaaa agagtggttt tgtgcgatgc tgctagctat caaaatggat
     7681 tagaaactga aaagacaaag atctatccaa tctcattaaa aggtgcaaac atccatttgt
     7741 atcatattgc tcaggaactt cacctattaa aaaaacatat ttcttgatga gaccttaatt
     7801 aggagacctt ctacaaagct ttttaattgt ctgtagaagg tttttatttg agtcctgatt
     7861 cgtgcgaaga tctacttata taacaagctt tctctgaaat ttttttaaaa tgctattgac
     7921 ttagagtcaa ctctaagtag tagaatagat aaagtgtaac aaatctattt tcattaaaag
     7981 aagatagtac ggaggagaga atgtattatg agtacagtat tacatgtatt ggatgcaatc
     8041 aatagccccg cagatgtcaa aaaattatct gttgaggaaa tgaaacagtt ggcagaagag
     8101 atgcgccaaa aaatcttgca aaaggacagc gatgtaggtg ggcacgttgg tccgaatcta
     8161 ggtgtagtag aattaacgat tgcgtttcat tatgtatttg attcaccaaa ggataaaatc
     8221 gtttgggatg tttcccatca gtcttatggt cataagcttt taactggtcg aaaaaatggt
     8281 tttgaaaaag gacattaccg tgaagtgagc ggatattcca accaacatga aagtaaacat
     8341 gactatttta cagtggggca cacatctaca tctatcagct tagcaacagg tttagctaaa
     8401 gcgagagatc tgaagaaaga atcaggaaat gtggtagcat ttatcggtga cggctcccta
     8461 agtggtggct tagcttttga aggattgaac aatggtggtg ttttacaatc caatctaatc
     8521 gtattagtaa acgataatga gatgtctatt gatgagaatc atggcagttt atataaaaac
     8581 ttagctgagc ttcgtcaaac caacggtaaa agcagcaata acctatttaa agcaatggga
     8641 tttgattatc gttacgtgga taatggaaac gatctggaaa cggtgatccg tttatttgaa
     8701 gaagtaaaag atattgatca tccgattgta cttcacgtcc atacggaaaa aggtcgcggt
     8761 tatcagccgg caatcgataa taaaatgaaa tatcattggc atgtaccatt tgatctgtcc
     8821 accggggagg caaagcaagt aacttctgga gaaacattta atatgatcat catggattat
     8881 ttggataaga aaatgcaaac aagtacagct cctattgccg cagtcaatgc agcgatacca
     8941 ggcgcctttg gactgaaaac attccaagaa aaacatccag atcattattt tgacgtgggg
     9001 atcgctgaac agcactctat cactttcgct tctgctttgg cttctcaggg agtacgtcca
     9061 gtcgtcttcc aatcaagtac tttcttgcaa cgggcatatg atcaattatc acatgattta
     9121 gcgatcaacg agaatccagt gattatttta gtcggtggcg gaacgatctc agctagtgac
     9181 cgaacacacc aaggaacatt tgacattcct tacttatcaa gtatcccgaa catcaaatat
     9241 cttgctccag caacaaaaga ggatatgatc tctatgctag attgggcgtt cgaacaaaca
     9301 gcgcagcctg tagcaatccg aattcctaca catggtgtag aacatggtac agctatcgca
     9361 aatgattata gtattccagc ctatcaaaca gtaaataatg gaggaaaagt agcaatttta
     9421 gctttaggcg gttttcttga attaggaaaa gaaacagtaa aagcttttag aaaaaaagga
     9481 atcgaagcaa cgttgatcaa tccaggaacg atcacagaaa atgacgtaga aacattagat
     9541 aaattaaaag aaacacacga attagtcatc acattagaag atggtagttt gtcaggagga
     9601 tttggcgaaa atatcagtcg ttattatggt gattcttcaa tgaacgtatt aaattttggt
     9661 gcagagaaag aattcacgga tagcgttcct gtagggcagc tttatgaacg ttatcattta
     9721 acaccaaaac aaatcgttga agatagtctg cgtgtcttga atattcaata attcattatg
     9781 ctcaccatcc agataattgt ttatactagt atctggatgg ttttttcatt ataaaaagca
     9841 aaggggtgga tgttatgtat cagcaagtag aagaatatgt caaaaagttc caagaaaaat
     9901 atcctagtta tcaatcagaa aaattaggtt tcttttcatt tggggataca gaaaaaatgg
     9961 cagacgagtt gagcgaacta gttgtaaaaa agataaagac tggtacatca agtggttacg
    10021 atctgtataa acaagagaat gagcctttac caaaagttgg tcaattagac gtggtcctga
    10081 atggaaaaaa tgaaccagtt tgtatcatca aaaatataaa tgtgaccatc ttgcctttta
    10141 gtgaagtcac gaaagagcat gcttggaaag aaggagaagg ggatcggaca ttagcttatt
    10201 ggcggaaagt ccatgctgag ttttttaaac atgcttataa agaagcgttg aacatagaat
    10261 ttacagaaga gaagctggtc gtatacgaag aatttgaagt aattttcttt tagtaagaaa
    10321 ggtctgctga aagaaatagc tcattattgt ctcctgaata gaaaagggtt acatttttat
    10381 atgaagctgt atttttatat aacattactg gaggagatat tgtggataca ctggaacaaa
    10441 tcaaaggcgg agtcgtcgtt tcttgtcaag ctttggaaaa cgagccgcta cacagttctt
    10501 ttattatggg gagaatggct ttggctgcaa aagaaggcgg agcagttggt attcgagcaa
    10561 atacgacaga agatatcaat gaaatcaagc gaatcgttga tttaccggta atagggatta
    10621 tcaaaagaga ttacgatggg tcagaagtgt ttattactgc gacagaaaaa gaagtggaag
    10681 aacttttgga gacgcaagca gaaatcattg cactggatgc aacttctcgt aaacgtccga
    10741 cagaaaaaac aactgctgaa cttgtccaaa tgattcacga acacggtcgg ttagctatgg
    10801 cagatatttc gacttttgag gaagcgcttc aagcgcaaga agatggtttt gatcttttgt
    10861 ctacgacttt agcaggctat acagaatatt cacgaaagac gcagacacct gattttgatt
    10921 tattgaatca aatcatagaa caagtcgata tcccagtaat tatggaagga catactgact
    10981 ctcctgaaca agtagagaaa gcatatgaat taggtgcttt ttcagtcgta gttggatcta
    11041 ttatcacccg tccgcaattg atcactaaaa gatatgtcga agccgcaagt aaagcaaata
    11101 actaaaaaac acgcacggca cagattcttg acaaaaaaga aaaattgata gatagtttaa
    11161 agcagattct aagaagatga aaaaagcatt acttttcatt ttgtcttcaa gatctgcttt
    11221 ttaacgaatt ttatagaaag aaggcgaaac catgtttgaa cggagtcctt cagaattgac
    11281 agaacgtgaa aattacaagt tgctgattgg ttcagtcatc cctagaccag tagcagtagt
    11341 aacgactaaa tcagcaaaag atatagtgaa tatcgcaccc ttcagttact tcaatatcgt
    11401 cagttcgaat cgacccatct tatccttggc gatccagaga aaacaagatg cagtaaaaga
    11461 cacagcaaag aatattcttg agacaagaga agctgttatc catatccttg atgaggacaa
    11521 cgtagaagaa gtaaataaaa cagcagccaa tctgcctaat gaggaaagcg aactgtacct
    11581 gactgacttg actttaactg attcagtaat catttcagta ccagcattga cagaagcaaa
    11641 agttcgtttt gaaacatcct tatatgaaca tgtcgagata cggaataaaa ataaagtaac
    11701 agctgattta tttttgctag aagtacagaa atatcattta aatgaaacag tctatgatga
    11761 taagacagga aggatcgatc caagaaaatt gaatgctgta agccgtttgg caggaaatga
    11821 ttatgcagca ataggagaaa ttttcacgat tgcacgtccc gaataaaaag gagcactagt
    11881 tatgttaaaa ataagctata aaccatcgac agattcaaaa gaaatgaaaa aagaatatga
    11941 aacagtgaat gattttctgc aagggcagta cttagaagtt ccgccattgc aagaccattt
    12001 tgtcgttact actgttactt tagatggtaa ggaaatagaa atgccagatc aaacgattag
    12061 cggattgttt aattacttta acaaataaat atttattccg aaaagtgaaa aaacat
//
LOCUS       DACENJ010000071        12070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.071, whole genome shotgun sequence.
ACCESSION   DACENJ010000071 DACENJ010000000
VERSION     DACENJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.071"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(129..1436)
                     /locus_tag="IW748_001405"
     CDS             complement(129..1436)
                     /locus_tag="IW748_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1104322.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1439..2020)
                     /locus_tag="IW748_001406"
     CDS             complement(1439..2020)
                     /locus_tag="IW748_001406"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1104323.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2136..2233)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2592..3884)
                     /locus_tag="IW748_001407"
     CDS             complement(2592..3884)
                     /locus_tag="IW748_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1104324.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(3881..4504)
                     /locus_tag="IW748_001408"
     CDS             complement(3881..4504)
                     /locus_tag="IW748_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104325.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4507..5343)
                     /locus_tag="IW748_001409"
     CDS             complement(4507..5343)
                     /locus_tag="IW748_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1104326.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5360..6247)
                     /locus_tag="IW748_001410"
     CDS             complement(5360..6247)
                     /locus_tag="IW748_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1104327.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6263..7546)
                     /locus_tag="IW748_001411"
     CDS             complement(6263..7546)
                     /locus_tag="IW748_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1104328.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8229..8912)
                     /locus_tag="IW748_001412"
     CDS             complement(8229..8912)
                     /locus_tag="IW748_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1104329.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(8930..9547)
                     /locus_tag="IW748_001413"
     CDS             complement(8930..9547)
                     /locus_tag="IW748_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1104330.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9557..10381)
                     /locus_tag="IW748_001414"
     CDS             complement(9557..10381)
                     /locus_tag="IW748_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1104331.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10374..10934)
                     /locus_tag="IW748_001415"
     CDS             complement(10374..10934)
                     /locus_tag="IW748_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104332.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11108..12064
                     /locus_tag="IW748_001416"
     CDS             11108..12064
                     /locus_tag="IW748_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1104333.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         3630 a         2651 c         2047 g         3742 t
ORIGIN      
        1 aacggtgttc aatcagctga ccctcggtat tagctcaaac aaacggcaaa atacgaggaa
       61 ctatttttcc gtttgtttct ctaacaatca ggtcaaaacg ctgatttcac aacctttctt
      121 ttgctctatt acttcttgaa ggcttcttcc aatatttctt catctttttt caatccatta
      181 aagatcaaat tcaatatgat agccgtaatg ctactcatta cgatgccatt tccagtgaac
      241 atttgcaatg tttgcggcat ttgactgaat aatgtcggcg taacattgaa acccagccca
      301 aatccaacag aaacagcaac aatcaataga tttttatcat tgctgaaatc aacttccaac
      361 agcatacgga ttccttggac tgccaccatc ccgaacatga ctaacattcc tccaccaagt
      421 accggctcag ggatgatttg tgctaacgca ccgattttag ggaataatcc taaaacaatc
      481 aagaagaatg cagagaaata gatgggtttt cttgttttga ttcccgaaag ttgaaccaat
      541 ccgacatttt gagaaaagcc agtataaggg aatgtattga aaataccgcc taagataacg
      601 gcaagtcctt ctgcacgata tccctttttc aattcttctt ctccaacatt tttcccagta
      661 atgtcaccaa gtgcaaaata tacacctgtt gattctacca tgctgaccat ggaaatgatg
      721 atcattagta aaatcgacca tacgtcgaaa gttggtttac caaaataaaa gggttgtgga
      781 acatggaaca acggcgcatc tccaatcgcg ctcagatcaa tcattcccat gaaagcagct
      841 aagatgcttc ctccgactaa accaatcaat accgcgattg aacgaatgaa cccttttgcc
      901 agcatttgca cgcctacaat taggagaatc gtcacaaatg caagtagcag atttgttttg
      961 tcaccaaaat cagctgctgt tgcattacct ccacccattt tctcgattgc tacaggaatc
     1021 aaagtcaaac caataacggt gataaccgtt cctgttacta taggagggaa tagtttttta
     1081 attttagaaa agacaccaga aactaacaca acaaagatcc cagaagcgat aatagatccg
     1141 tagatagctc ctactccttc attggttccg atcaagatca gtggagctac tgcttggatc
     1201 gcacatccta aaacgacagg caagccaatt ccgaaaaatc ggttaactgt caattgcaat
     1261 aatgtcgcta atccacacat aaagatatca attgaaatca aataagtcat ctgttcttga
     1321 ttaaagccta gtcctgtacc gatcaaaaga ggcaccgcta ctgctcctgc atacatcgct
     1381 agcagatgct gcagccctaa aaccgcagct tttccatttt gtgtttcttt ttccacgatt
     1441 atgcgtcctc ctcaagaaac tcaacttgtc cattcgtcaa ggaagcaata cgcgcaagag
     1501 aaacgactct caatcccatt tcttcaagta actgacgtcc atcttggaaa gacttttcga
     1561 tgacgatccc gattccttct acttgcgctc cagcttgctg gcataattcg atcaatcctt
     1621 ttgccgcttg tccgtttgcc aagaaatcgt cgataatcaa tactttgtct tctgaagata
     1681 aaaatttacg agaaatagaa atcgtacttg tcacttgttt cgtaaaagaa taaacagaag
     1741 ctgtcaataa ttcttcatcc atcgtcaaac tttttgcttt tctagcgaag atcattggaa
     1801 catctaattc tcttgcagca aataaagctg gtgcgatccc agaagcttca atggtcacaa
     1861 ctttcgtgat tccttgttct ttaaatactt ctgcaaaccg ttttcccatt tgttccatca
     1921 atacagggtc tacttggtga gtgacgaagc tgtcgacttt taatacgccc tcgcctaata
     1981 tacgtccgtc tttttgaata cgttcaacta attctttcac tttatttgct cctttatcat
     2041 ttattaatac cgaaataacc gcttttgtgc aaaaacctac tatgtttctg ttaaagaaaa
     2101 acaccctcca ctttatcata agaaaagaag ggtgaaatag ccaatctttt acttatagtc
     2161 cagcatttac ggtactggct agaaactgtc atccatatta tgacgatata taagtaggta
     2221 tgtaaaacta ataatagtgt aaattcattt tcttgcaact gttttgcacg tgaaggcagt
     2281 ttgccatatt tgaataaaaa atgttgtttc aaatattgaa aacagtctaa cactactttc
     2341 tgaaactgac aacgtggttt acttattttt ttgtaaaatc taatgatttt taatgcatta
     2401 ttcctttttg ttcgggttta aatagttatt tttattttct cctatctaaa aaagcagccc
     2461 acataaaaag aaggcatgac aaaaagcatg tcatagcccc tttttccgaa taaatagtgt
     2521 tcaaaagaca gcctatcggc aataaggcaa gattttataa actacgggaa gtaatttccg
     2581 taaatctatt cttattcttt taagctgaca agctcgtcaa tcactactgc ttttcctgaa
     2641 gcaatagaat gatttgctgc gattcctgtc aaaatcgaca aaataccatc tacgtgagtt
     2701 gctgcccgat tccacggatc ttcttctttt tcttcaccaa atagatcatc taataaacgc
     2761 ggatctcctc cgccatgtcc gcccgcttct tgttcgacga ttacctcata tggctcaccg
     2821 aacatcggat agacatggat ggactgaaga acggctgctc cttcatcctt tttatctcca
     2881 cctgcattga tgtaagactt ttcgatcact ttcatttcga tccgtccttt gctgccattg
     2941 aaagaaacat tgaatccttc ccacggcata tacgcgttta gcgaatacgt caaaatcgca
     3001 ccagtttcat atttgaccgt tacgcctagt gtatcttcaa tagagatatc atcggaaaat
     3061 acactgcggt cacgaatata tccactgtct ttttctgctt ctaaatacat ttctcgcaat
     3121 tcctcattgg ctgacaggtc tatagcaaat ggatcagctt tagctagttc gcttccatag
     3181 catcgactgt aaaatatttc tactccgcgt ttctccgcat tatgcatccc ataaaagttt
     3241 aatccaccga atgcaaatac cttttgggga cggctgttca accaaaaatt cactagatca
     3301 aaatgatggg tagatttatg aacaagaagc ccgccgctat tgtgcttatt tcgatgccat
     3361 cttctgaaat aatctgcccc gtgttgcgta tttaacagcc attcgaagtg gatagaaaac
     3421 acatcaccaa tcacatcact ttctatcagt tcctttatct tcgtgtgata cggtgcataa
     3481 cgataattaa aagtgactcg caggcttctt cccgtctgtt tttggaccgt tattatttct
     3541 tgagcttttt cctcatcgat agtcagaggt ttttcgcaaa taacatcata tccttttttc
     3601 attgcacgaa tgatataatc atgatgcgtt cgatctactg acgtaataat gacttcagcc
     3661 ggccgtacgt catccagtag ttgctcaaat tgtgatggca tacatgtctt tacttttttc
     3721 tgtccatact tgttcacaag tatccgattt gcataatcca tccttgtttg agatacatca
     3781 caaaaaccga ctagttcaca agtttgatga tacgtagagg caattgcctc ataaaaaaaa
     3841 cgcgctctcc caccaagtcc gacttgtaca tattttttct tcatttaaag tcattctcct
     3901 tttcttttaa gcaaaatact gtgacaaatg gcagccctgg atacagtaat gcagtgagta
     3961 aaaaatcatg ctgtatacac cagaacagtc catacaaaag gcataaatac cacagaaacc
     4021 ccttgacgtt ttttgacaac agccagatat tccttttgat caaataaaca agactatctg
     4081 ctttatcttc tttgcagata tcaattagcg tactaaaaaa actatgggat acaaaaagca
     4141 acagacacaa tagaaaagga atcagatacc aagctccaat ctttcccact aataaatacc
     4201 aatcaactgc aaaaataatg acaaacagct gaaacaacgt aatgatgaaa cacatccgtt
     4261 tgatttttcc tttcctccaa catacctttt tttctgccaa gtaatggaca gatagataat
     4321 aagaaggata catcatcaaa aaataaatca gatagacaac acgagcagac agcactaatc
     4381 ctaaagtgaa caaacatcct agaactaaag cgaaaagagc attactgata agactggtta
     4441 aaaaaatccg acagacatag tagttgagct tgtaaagaaa catctcttct attggctttt
     4501 tcatattcag cccttcagac cgcttgtact gatcccctgg acaagatatt tattgaagaa
     4561 taagaaaatc agaaaaaccg gtagcagcga caacgtagac atcgcaaaca tactcgcgta
     4621 agtcgtacta gatccagcgt ctgcaaataa tttgatggca atcgataccg tgtaagatct
     4681 cggcttgttt aaataaatca atggtcccat gaagtcgtcc catttccaat aaaactggat
     4741 gatgatcgtc gtgatgatcg aaggtttcag caaaggaaaa ataattttgg taaagacact
     4801 atacttgctg catccgtcaa taatcgctga ttcatctaac tctttgggga tgctgaccat
     4861 gaattgcatg atctggtaaa tgaaaaatgc ttgtccaaaa aaatgtggta aaatcagcgg
     4921 cacatacgtc ccaaccaaat tcagccgatt gtaaataata aactgcggaa tcaaaatcac
     4981 ttgtgcagga atcatcatcg ttactaacat gacgacgaac cagaatcttt ttcctttgaa
     5041 atcgattctc gataatgcgt aagcaataca agtcgagctc agaactgtcc ctatcgtcga
     5101 aactgcagag atgacaaacg aattcctaaa gaaagtaaaa aatgaaattc cagcaaaccc
     5161 cgcccatcct tttgcgtaat tttcccattg aaaagtctct ggaatcaaat tcagcgaacc
     5221 actcataatc tcttgatccg ttttaaaaga acctgcgatc atccataaga ccggataaat
     5281 catgactaga cctaatagca aaataaaacc gtattgaaac catttcgtta ctgtattatt
     5341 catttcttcc tcctctcatt cacacaacga cccatttttt tgatgatttg aaaatcagaa
     5401 aggtgacagc tcccataatc aacagcatga tccacgaaat agcactagca taccccatag
     5461 aattaaaaga aaaagcttgg ttatacacat aaagagcata aaagttcgtg gcatcattcg
     5521 gaccaccttg ggtaatgata aatgcctgcg taaaagtcat aaacgccgcg attgtctgca
     5581 taaccaagtt atacaaaatg atcggcgaaa gaagaggtaa cgtgatcttg taaaacgtct
     5641 gccatttgtt cgctccatcg atctctgccg cttcataata cgagcctggg atctctttga
     5701 tacctgcagc aaagatgatc attgccgaac cgaattgcca agcagacatc aaaacaagag
     5761 ggatcatgac cagtttttga tcgctaaacc aggaaagcgc aggtgcgcct aatcgttcca
     5821 ggaaaatatt cactaacccg tttttagaaa acaactcccg ccaaactaga gcgatagaaa
     5881 tactgccacc gatcaaacta ggcacataat acaaagaacg aaagaaagtc gttcctttga
     5941 tattcctcgt taaaagataa gcaatcaata gaccaaagat cagtttcaca ggtacagatg
     6001 caaatacata gagaaaagtg actcggatgg agttcaaaaa acgggcatca ctcagcatct
     6061 ttatataatt atccaagccg atccatgaga ctggatttcc caaacgataa tcggtgaacg
     6121 aataataaag tgacaagatc atcggtatca ctgttatcaa tagaaatcct aggatgaatg
     6181 gagaagcaaa tacgtaaccg accacattgt cttgatccaa gaaccgtttt agtttttctt
     6241 ttttcatgcc ttcctcctgc tgctatccta gaatgtttga cgcaaactca taaagactct
     6301 ctgctgcttc ttctgctgtt acttcttcat aaatgacttt ttcaactaaa agattgtatt
     6361 ggtcttgaat ttccggattc aaagcactat caaagacatt cacttcgccg ttgtccatat
     6421 cggaaatcat ttctaaataa tcaaatgtcg cttgcaaagt tgcatcatct gcacgagcga
     6481 gctgttctcg aatattcgac atgattggta caccacgttc tccttttaaa atttcattgg
     6541 cttcttcact gttttgaaag aaatcaataa acttagcagc ttcttcttta tgggctgagc
     6601 tttttgccat actcagcatt tgcgaagaac gtaaagggat accagattct ccatctgctt
     6661 ttctgcgagg tggattaatc aactgaatct ccatatcttc aggagcccct gacaatattt
     6721 ctgtcaactg gttacttgat agaaacacca ttgccgcttc tctatcccta accgcctgaa
     6781 aagacagatc cgtcgtactt gcagctccac cacctggttc tggatgggcc cctttgtcta
     6841 ccagattttt acgcagctgg aaaaaatctg ttagatagtc tggattttca aatcccaagc
     6901 tgttagatag atcttcccga ttataaaaat tcaatccttt ctcaacttgt ggtatttgaa
     6961 taatacagcc agctataaaa tcttcgaatc ttgaaatagc atagattcct aattcttcag
     7021 tgatttgata gctgatttgt tcaaattcat cccatgtcca atttgaagct ggttcaggga
     7081 tatttaattg ttcaaatata tttttattgt aagcaatcgc aaacgaattc accccgttag
     7141 acagtccaac taattgcccg tcgtaagtcg tcgttgctaa ataggattca tctgcattag
     7201 aaatatcaat tatcccttgc tccacatatt catctaatgg ttcaatctga ttgatgtatt
     7261 gaggaaattg attgttcagt tggaaaatgt cccagatatc atctgatgct gctagtgtac
     7321 ctaattttgt gtaatatcct tcataatcat aaaattcata atcgatcgtc acatggggat
     7381 ttttttcttc ataaagctcg attactttta ctgttgcatc gtgtcggact tgcgaacccc
     7441 accaagccat gttcagcgtg atttttttgt catcttcttg cccacaagcc gtcaagaaga
     7501 ataacataac acttaaagct aagatgattc tcattctttt cttcattctc tgcacctcta
     7561 cttccattgt tatttttcaa tatactgtat ttttatacag aacatattta ttaaacgatc
     7621 tttttcaagg ggaataaaaa ccaagacttg ccttatcgtt ctgcctagga tgaacaactc
     7681 aatactttca agaaaaatta gatagtgctg attcttcttt catcgctaaa aacacttctt
     7741 gcttcaatga tgggaggata attacgcaaa gaaaaaagga tatcagacaa ctgcccaata
     7801 tccttgacat agctaaataa gcatgcttct tcgtgtgcgc tcttccctaa aaaacagaaa
     7861 aataactagt aattctgtat gaaattaggt acaatataca cgtcgatgca caagcattga
     7921 ctatggcaac ttgattggtc agttcccgct gacttttcaa gccccgacaa tttagctgct
     7981 actaaattgt cgggtgcctt ttttagtttt tattcgtttt tcattctata gaaagcgctt
     8041 caataaatca agacattttt ttgttagaat gctaataata acaaaaggtt ttaactcttc
     8101 ttatgtacat tacaggtaaa aaaacaaaaa aatttctctt ttttttgaat tctgaacaaa
     8161 tagtactgga gtacaaaaga cgaggaagag ccactatctg ctcttcctcg tcttttgatt
     8221 ctcacctgtt attcatgatt ctttttctca gtttcccgat atttttccat tttacgacta
     8281 aaaagctctc cgctacttgg tggcgtatat gtgatggcat ttgctcctgc ttgaatggtc
     8341 tcagcaatac tttcttccgt tggaccacct gtcgcaatga taggaagttc aggatatttt
     8401 ttacgaaaat ggcgtactgc ttctgcagtt tctttacctg cactgatatt gataatcgaa
     8461 ataccagctg ctaacttttc atctatttct gtatatttcg acgttaccgt actgataact
     8521 ggaatatcaa ctactcgaca aacttctttt actgtctcta aaggcgtagg accgttcaaa
     8581 acgaccccta acgaaccttg cgcttctgcg aacatgctca tgtaggaaga acgagcacct
     8641 tgtgtcaaac caccacctac accggagaaa actggaatgt cagctgcctc aataatgctt
     8701 ttagtaatcg ctggatgagg tgtaaaagga tacacagcaa ttactgcatt cgcatttgta
     8761 ttacgtatga tcgcaatatc cgtagtaaag atgatcgaac ggatcaattt tccaaatatc
     8821 ttgatcccgc ttgcttctaa aatgatttcc ggtactctta cgatatcttt tctcaattcc
     8881 gtcatgattt ctggcgtaaa ttgttctgcc atcagccact tctcctttgc tatctccggt
     8941 catactgatt caattgatac gtctcgatga cctggatgat tttttgcgca taaccggggt
     9001 ctgtcgcata acctgcatct tgcaatgctt gtgcggcttc ctgatagttg gtggccgtaa
     9061 tcactttttc atacagcgca ggattccaat ctactccttg gacgaacagt tgtgtatggt
     9121 catccatgga ctgttcccat gaatcataca ctttgaagtc tccttggata gttatccatt
     9181 cttcgttaac aaattccttt gtttccaggc ttactttctt ctgatcccca taagccttga
     9241 tcccgaaaag attattgtat ttgctagcca aggtactttt tccccaattt gattctaata
     9301 tagcttgtcc taaaatgata ctaggaagca ctccataacc atcttgtagt tcttgggcat
     9361 gcggaacaag ccgttcaata aattgctgat gggattgcgc ttcattttct tgctgattat
     9421 cagtaaaagg agaagacaga ttcttcaagg aaaaaacaaa ggctaagagt atcactgcta
     9481 caccggcaac cactgctggc caacggatat tcttgcgctt ttgctttttc cacttttttt
     9541 tcgccatgat caattcctac ttttcattta attcattcag atattcttct agcgtcaatt
     9601 ggtgtttggt gatatactca gcgttttctt taccgacata acgtaagtgc caaggttcat
     9661 atgtgatctt agttatatct tcgtgtcctt ttggataacg aacgataaat ccatactgcg
     9721 cagcattatc tgcgatccat ttagctccag gctgatctcc gtaacttgca tccaatacag
     9781 tagaaggata actgttgtac cagttttgat cgacgacatc gactgccaga cctgtgtggt
     9841 gttcgctgta tcctggctcc gtaatcgttt ctttagtaat ctttgtcgcc tcctcttctg
     9901 atacaccttg acgactcatc acatcttgga cattttgttc gaaaacttgt tgttgggatg
     9961 atacagaccg gtaagctgag accatcacta atggaaatcc agctgcttca gcagccttgg
    10021 caaatgcttc atattctgga gcaatgcgtt tgtcgatcaa gtatccgtta cttaatgatg
    10081 ccaattgagt agactcatca atttcttttt ctaatttgtg atctggacca accaaaacta
    10141 gcttccaatc gtctgtttta acatctggca attgtttgga cgtacgatct tttgtttctt
    10201 tgtcctttac cttgctgctg gttgtttgac tgttcttttc tttactagca gttgttttgg
    10261 tgcttgttcg ttcgactgct tgattgccgg cttgaacatg cgaatcatca tcgctaaaaa
    10321 taacatagcc cattgctgcg atcataatca caactgctcc aaatcctaaa aatttattca
    10381 cttgaaaaat cctttcgttg ttcttcacgt tccccattcg cctcatgaac gtaagtttct
    10441 tccagatttt cattcaccca atcgagaagt tcgatttttt ctccttcgat ctgtttttga
    10501 acatctgctg gcagacgaaa attcaaaata tcatcatagc cccattcatc atattgaata
    10561 cttactttca agtcaaggta tctaccagcc tgctgattat ttttcaaagg gatcagctcg
    10621 acttttgctg ctttgctgct aagccatttt tgcgcgatgc gtgttttgat ttgcttgtac
    10681 tctgcaacgg caacgtttcg tttttcggga aaaataatcg tttgacgcaa aatcttttgc
    10741 gttagcatcc attcataatc agtgaaaaga ttttttactt tctgcatcgc ttcagatggc
    10801 agatttttct cgccatcttt ccatttttgc cattgttctg gactgatcag caagtattca
    10861 tctaaaaaat ttttttcagt ttcaaactgt tctaataata cactcataat aagatcaata
    10921 tatacttgat gcacgtttat cccctccttt taactattct acttaagttt tgatcaaaaa
    10981 gcacctctta aatcgctgct ttcatttttt ttaatgtttc aagtcctctc ctctttattc
    11041 ttcgtgattt ttgttataat aatagacaga caagaaactc aatccaacta tgaaggagcg
    11101 ttttcagatg aaaaaatatc aatggggaat cgttggtctt ggtaccatcg cacatgagtt
    11161 tgccgaaagc ttcaatcaag agacgagcga actaacagca gttgcttcca gaacttcaga
    11221 aaaagcggaa aacttcgctc accgttacaa tattccaaaa gcttacggaa gctatcaaga
    11281 aatgttagac gatgctgaaa tcgacatcgt gtacattgct gtaccaaata gacagcatat
    11341 cgaccatatc ttggcagcat tggaagcagg caaacacgtt ctatgtgaaa aagccatcac
    11401 gatgaataaa aaagaactgg ctgacgctat gcgcttagca gaagaaaaga atttgatctt
    11461 agcagaagcg atgacaatct tcaacatgcc actttatcag cagttacgct caataatgga
    11521 tacaggaaaa ctgggggctt taaaaatgat ccaagcacct ttcggcagtt acaaagaacc
    11581 agaccctaaa aaccggttct tcaatccaga attagctgga ggagctctgt tggatatcgg
    11641 tacgtatgct gtatctttcg ctcgtttctt tttgagctct caacctgaag tagttgcctc
    11701 tacaatggtt ccttttgaaa caggcgtgga cgaacaatcc gtcactatct tgcgtaacaa
    11761 agaaaatgaa cttgctgctg tcagcttgac ttttcaggca aaaatgccaa aagtcggtat
    11821 cgttgccttt gagaacggtt atgtgacgat tgctgattac ccgcgtgctg atcgtgcgga
    11881 gatcttattt acagatggta caaaggaatt catcgaaagc gggaatacat ctgaagcaat
    11941 gaattacgaa ataaagaata tggtacacat gattgaaggt gacttgccca atcgctctct
    12001 cttcttgaca aaagatgtca tcgaaatctt ggatcaaatg caagttctat ggaaaaatat
    12061 gtaattttaa
//
LOCUS       DACENJ010000072        12055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.072, whole genome shotgun sequence.
ACCESSION   DACENJ010000072 DACENJ010000000
VERSION     DACENJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.072"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(139..480)
                     /locus_tag="IW748_001417"
     CDS             complement(139..480)
                     /locus_tag="IW748_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1104334.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(548..2719)
                     /locus_tag="IW748_001418"
     CDS             complement(548..2719)
                     /locus_tag="IW748_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1104335.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGATETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            2930..3787
                     /locus_tag="IW748_001419"
     CDS             2930..3787
                     /locus_tag="IW748_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1104336.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(3858..4715)
                     /locus_tag="IW748_001420"
     CDS             complement(3858..4715)
                     /locus_tag="IW748_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1104337.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(4700..7402)
                     /locus_tag="IW748_001421"
     CDS             complement(4700..7402)
                     /locus_tag="IW748_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1104338.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(7415..8704)
                     /locus_tag="IW748_001422"
     CDS             complement(7415..8704)
                     /locus_tag="IW748_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1104339.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            8854..9900
                     /locus_tag="IW748_001423"
     CDS             8854..9900
                     /locus_tag="IW748_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1104340.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            9902..12055
                     /locus_tag="IW748_001424"
     CDS             9902..12055
                     /locus_tag="IW748_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1104341.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         3316 a         2550 c         2199 g         3990 t
ORIGIN      
        1 actcagagtt aaacggtagg ctcacgacct cttggtaaat ggtgttcaag agacagccta
       61 tcggcaataa gaagcaattt tgtcatcgct caaggccgaa cgtctctttc acaccctctg
      121 ttgtttcata aaatagtttt attcgctgta aaggtcgcga actggtgtca tgatgatacg
      181 gttcaagtca ttgatgattg tactgaagcc ttgctctttt tgcatcaagt cattgattac
      241 ttcttctgat tggattttca cagccatttc ttgtgctttc attgcatctt cgtcgctgaa
      301 ctcttgtcca ctcatttgtt tttcttgtaa ttcgatttgc atcgcttgga agtctttgaa
      361 cagtttgtgt gcttcttcgt ttgcttgtac tttctcgtac gcttctttca acgctttgaa
      421 ttctggtaat tcacggatct cacgttcgat ttgattcgcg ctatcgtaga tgtttgacat
      481 gttatgccct cttttctttt agtagttact ctctattgta acatggggtt attcgttttc
      541 aaaggggcta ctctccttga tatttccgcc agaggtcttt agccgcttca ccaattccct
      601 gaacgccttc tttcaccata tcgccgaagt ttttcagccc ttcttttgct tgttcaccat
      661 attgattcaa gttatcttgc caatttccag actctccatt agaatttcct gtgtttccaa
      721 cttgatctgc agctaccact tgtcctcctg tggcataggc atctgctaca ctaaatcctg
      781 tttttgaagt atacggaagg atgccttctg ccgctgattt gaagactttc ccgacaacat
      841 ttccacttgt cccttccaaa tagtgcagct tgctggattc ttcgtaaccc atccatgtcg
      901 aaatcactac gtctggcgtg taaccgatca tccattggtc atttgctttt gtggcatcga
      961 aattagtttc tgtggctcct gttttccctg ctatcgtata acctgcagga ttcgcagcag
     1021 ccgctgttcc atttgaaaat gtaccaagaa gcatgctggt catttcatcg gctgtttcct
     1081 ttgatatgac ctgttttggt tttttgtttg tgttatcgac gatcaccgct ccagtcgaat
     1141 caacgatttt cgtgataaga tgcggcgtat acatctgacc atcattggca aatgcactgt
     1201 aagcacccgc catgatcaat ggagatactc ctttttccag tccaccaagg gctaagccat
     1261 aatacttatc agattttgct aaaggcaggc cgaattcttc tgctttatca tatccttttt
     1321 gtaaaccgat ctcatgcaaa agccatacag taggcaaatt caaactttgt gcaacagctt
     1381 gatacatcgg tacttctcct tggtatgtcc cgtcaaaatt atgcgcatcg taataacttt
     1441 gcggttcgtc tttcaagata ctggaaggct tgtatccagc ttccagtgct ggtgtatata
     1501 cgcttaatgg tttgatcgtt gatcctggtg aacgacgcat ctgtgtagca aagctgaatc
     1561 ctctaaagac atgatctcct cttcgtccta ctagtgcttg tacaccacct gtattcggat
     1621 ctaaagctac tgaagcagat tggaccatgg caccatctga agcatttgct gggaataatg
     1681 cactgttttg ataagtagct tctaattgct tttgataatc ctgatctaaa gctgtataga
     1741 ttttatagcc tttattcatg acatcttctt catcaagacc atactcgtta actgcttcat
     1801 cgatcactgc atcaaaataa taaggataac gataaccaga atctgaatta tcgtatgtgt
     1861 cattaagata cgcgctgata tctgtctggc tttctgcttg ttcttgttct tttgtgattt
     1921 ttccgttttc ttccattaca cttagtactg tgtttttccg atcattcgca ttttttgggt
     1981 gatcgatcgg attataaata ccaggacctt tcagcattcc tgccaaagta gcagcctcag
     2041 acagcgtaac attcgttgca tctactccaa aatactttct ggaagcgtct tggacacccc
     2101 ataccccgtt gccaaaatag gcattgttaa gatacatggt caagatttct tctttgctgt
     2161 acttcttttc gatctcgatt gccaaaaata attccttcgc ttttcgatca aacgtttggt
     2221 ctaatgtcag atacgcattt ttggcaagct gctgagtgat cgtacttcct ccgccccctg
     2281 atgttccgcc agacaagacc attcggactg ctgcacgggc aatccctttg atatcaaacc
     2341 cgtgatgctc gtaaaaattc ctatcttctg tcgagattac tgcatcttgg atatagggag
     2401 aaatcgcgtt taattctaca tatgttcctt tttgggcgta cagtgtgcca gcttcttcgt
     2461 ttttcttatc ataaatgact gtcgattggc ttaatcctga ttttaacgtt tcgacatttg
     2521 cttgttttgc taaaataaag aggtagatac ttgtcactaa gacacagatc aaaccaatca
     2581 gcaaaatcaa tttgttcaca tgatattttt tccagatgcg ctttcgccat cgatggaatt
     2641 gtccgagata tggtctgatc caacgccaaa aactgatcaa agcagctttt atcttttcta
     2701 aaatgctttg aaaatccatt tgcttcttct cctgtctatt tgattcaatc gtcttatttt
     2761 accatagtga aagagtgttt tcttcctgct taagttgtag ttcaatatgt ttttttacgt
     2821 tagtgcaagt agagtaacat aagaatgaac agaaaaatgt aagttttttc ttaaatatgt
     2881 tttaaaaatg gtacaattag tctgcgattg cactgaaagg agtacacttg tgaaaatcac
     2941 gatcacttta cctatgaacc atcctgaaac aacaattcgc gacttgcttg aaaaggaatg
     3001 gttagttcct agaaaggttc gacacttttt gcgtatacgt aaaaacgttt ggatcaatca
     3061 acaacctgct ttgttccatc atgaagtaaa gggcaatgac cagatcacat tgttattcga
     3121 agaaacagat tataattatc aagaagttca attaggagat gcgaagcaca tccgtgtttt
     3181 atatgaagat gagcatttga ttatagtgga taagcctgca ggaatgaaga ctcatccaaa
     3241 tgaaccgaca gaaaacagta ccttactcaa tcatttagct gcttatctcg acactaaaca
     3301 gcaacgacct tacgttgtcc accgcttgga caaagaaaca agcggtgccg ttttatttgc
     3361 aaaaaatcca tttgtcttgc cgatacttgg aagaatgttg gaacaaaaaa tgatctatcg
     3421 gagatatcaa gccatcgtat ggggagaaat tgccaaagat atgaccatca ccaataaaat
     3481 tggacgagat cgccatgatc ggagaaaaag ggtcgtagat gataaaaatg gtcagagcgc
     3541 cttgacacat gtcactgtca acaaagtact ttctgaacaa acacaagtct attgtgtttt
     3601 ggaaaccgga cgtacacacc agatcagagt tcatttagct cataagggac atccgatcgt
     3661 tggagaccct ctctaccaaa atagaacagc tgaacgactg atgctccatg cattagaact
     3721 gcacatgatc catccattta ctcaagaaaa aatagtcggt aaagctttac ctggattatg
     3781 ggaatagaaa taaaaaagaa cagagtctta tcagcagata atgataagac tctgttcttt
     3841 ttgtgtaatt tgtctgctta cttttgatgt tcgtgttctg ctaacagcaa actaccgata
     3901 atcccacttt cattcggcag tccccaacga acgatatatt cacttacttg aggtgtttcc
     3961 atataaccac ccatcaattc aacaaattgc tgacggattt tttgaaggag atgatcctga
     4021 ttcattaccc caccgcctaa aataatcttt tcaggtgcta acgtcaacgt atagttgatc
     4081 agtgcttgag caatataata agcctgaata gcccaaacag gatgatcttg cggcagatct
     4141 tgccctttga tccctgtgcg tgcctcaata gacggtcctg ctgctaatcc ttctaaacaa
     4201 tctttatgat aaggacaagt accttcataa tggtcatctg gatggcgttt cacccaaata
     4261 tgtcccattt caggatgtgc gatccctgaa aaaatttctt cattgatcac gacacctgca
     4321 ccgattcctg ttcccacggt aaggtaaata caactatttt ttccttgtgc tgctccttgc
     4381 ttcaattccc catatgcagc agcattgaca tccgttgtcc aagcagcagg aatattgtag
     4441 cgttccttga ttgtgccaag aaaatcgaat tgtgcccacc cttttttggg agttgccaaa
     4501 acgtatccgt aattatcttt tgttttgtcg ataccaatcg ggccgaatga gccaatcccg
     4561 attcccttca attcataacg atcaaaaaat tcaaatacct gatccaatgt ttcttctggt
     4621 gttgtggtag ggatacttaa tttttctaaa atttctccac catctgaaac cgcacacaca
     4681 aattttgttc cgcctgcttc tattccgcca tacattcgtt ttcctcctcg aatccgattg
     4741 tccgaatctc ataaggttgg attattggga caaccgcttg cttcgttggt tcttcaagca
     4801 gattcaatat gacagttttt ccattgtctt tagttatttc caatttttct aaatgactag
     4861 acatattgaa acctcttact actactttat cactaaactt gctgcgtttc aaagctgtaa
     4921 ttgcaaacgt ttcacttttt atcgttaaat attgttgttt tgaaatcaat gttccttcat
     4981 gatgtttgat ttgttgagtg gaaaaaggaa tctgtgctgc gtatgcatgc aaataagtag
     5041 agaatttttc ttctggtcca tgtagttcaa tgctgtaatt gaaccgatgc tcacctagac
     5101 actgtgcttc tggtgttgga aaatatcccc aatcgcctag ttctcccaca ctgcgaagta
     5161 gcgtcactgc gatttgtccg tcttctgtta cttcatattc attcaaacca aagtttccga
     5221 ctgttacacc gtactcttca tcatgaatat tgacaaatgc ttgctgatgt tgcggattcg
     5281 tggggttttc ccaactttta gacacatggt ttgggcgctt caccacttca aaaatactat
     5341 ctgcttcgtg atgttccaca tgtaactttg taggaaacaa gacacgtaag cgatgatctt
     5401 tcatctgatt gtcgagagtc gtttcaaagt cgattttttt actatctttt ctgattgtca
     5461 tgattgtttt tatttgaagg attcgttttt ctttggatcg ttctgctttt cgataacgaa
     5521 attccacaac catctgctgt tctttttcta aacgctcgtc tgcagaaaca gggatttcca
     5581 tttcctgtag aatacttatt tttgctattt ccggtgtatc tactaatact tttacttcag
     5641 agttttcttt gttactggat aggattggct gatcacagaa aggttgttta aagatatact
     5701 catttcctat atcgccgata tcttcaaaaa tatgcagatt attcaacttt cgtttgtttt
     5761 tgcgatcttc cattgtaatg gtaccatttt tttcaatcgt caaatgtaga gggccgtttt
     5821 cgatgattcg tccactctga tgaatcattg tttccttttc ttctgctttc gcttcccctt
     5881 ctacaagcgc gaatgtttcc caagaaaatg ctggcatgtt ttctaatggt aaagtcactt
     5941 tgatcattct tgccatatat ggcacacgga aacggtcttt cggcaaatca taaccaaatt
     6001 ggacagtttc ttcagataaa atagctggta tagtggcccc actttttgtt tctacatgat
     6061 acttacgttt aggcaaattt tctaactctt gatatagttg ttcaggtatt ccttcagcga
     6121 atctttttct ttctaattcg atggttattt ctgcttcacc agttttatcc attcctgcag
     6181 tatttacaat gacaaatgga aagctgtctt ttgggaatcc ggtcgtatcg attgctgcgg
     6241 tcagctgttc taaggaatct tgtgcaagat actttccaac ttcatctgct ttttcaaaac
     6301 gaggtatcat ttcccgatgg acagaatcga ctgaacaacc gcaaatactg tcgtgaggat
     6361 gattttgcat taatgtcttc caagcataat ctaattgatc atggggatag tttccactaa
     6421 cttcataagc catcgtcgca agtggttctg taatattttc tagctgtcgc tggaccttgg
     6481 tattccattg ttttaagtaa acccgagcag atgctgtatt ggctaaagta taccagccat
     6541 ccgtttcttg acttgtcaat tctccttcta ctgagcctaa gtcttctggc acatcttttt
     6601 gaactgcttc cagatagtca gtaaaattag aatgaataaa ctcgtaatct ggaaacagtt
     6661 catttgcgag atggattgct tttgatatat ctttttgtac cggttgatga tcaacaccat
     6721 tcatcatcaa taagtggttc gtagaggcat attgttctgc atccgctaat ttttgcttcc
     6781 aaaaggcaag agctgcttct ttttctactg gaatctcatt cccgttgcta taccaattgg
     6841 caaatagcag accaaagatt gctgtttgat caggtccttt ccaccacatc tctgagtatt
     6901 gggaagaata gttttctgct tccaaaactt gattatcaaa gccaattggt tttacaccac
     6961 ggccaaacgc tgctgctgaa ataccagctt gtttcattaa ttgaggcgtc tgtcccatat
     7021 ttccaaatgt atcgggaaaa tagcctaaca tgacaggggt tccccattta cggctttctt
     7081 ccatgccaat cagcatattt ctcacgttcg attccgagct gataagaaaa tcatcttgca
     7141 agatataaaa tggtccaatt ctcaattttc cttcattgat tgcttgttgg attgcctgtc
     7201 ttttttctgg ccttacttga agatagtcat ctaaaataat cgtttgccca tccaaatgaa
     7261 aactgttgaa ggacggatca acttcaaata gctctagtag atcgtctatc aattcaatca
     7321 gtcgcatgtg atgctgttcg taagccatgt accattcacg atcccagtgg ctatgagaaa
     7381 tgatatatac tttctttttc atttacgata cctcctactt ttctactcta atatcaaagt
     7441 aatccatgac taattcgcaa aacatcatat ttgcccaaga aaaccattct ctcgtataat
     7501 tttcaggatt gttcacatcg aatccttcgt gcataaggtg tgttcctgcg tcagttgcta
     7561 caagtaaatt caatatccgc tctttttcag atttgtctga cgttgtcatt ccttccatag
     7621 ctaatgcaat cggccaaacg tatttttctg gcgtatggga ggatccaatt ccttttgcgt
     7681 actttccttc atagaaatat ggattttctt tactcaacaa tgttcttctt gtttgcagat
     7741 aatcttcctc ttgttcagaa caatatccca agtaaggtgc ggcgatcaga ttgggcacat
     7801 tactgtcatc catgactgtg gcgtttccca ttccatctac ttcatatgca taaatcgttt
     7861 gccctttttg attaaccgtt cgtccgtgat tctgaatccc ctcttggatc tctttcttca
     7921 atttctgtgt ttgctgtaca atttctttat catcgagtat cgtttgaaaa atttcttcca
     7981 cataacctaa cacgaccacc gcaaacatat tggatggtac cagatatcca taacggcaag
     8041 tatcatcgct aggacggaaa ccagaccatg tcattcctgt aaaagctgtt tttgcacctc
     8101 gcccgtcatt gatcagcgta tcttcttttc ttgtcgtgtc tcgtacaaat gtataaggag
     8161 agtgttcatg atcttgctct gttttgaaca cgtccaagat ttttttgata ccttcaataa
     8221 aagattgatt gaattgatca gtttttcctg tatttttgta aagaagatag gccaactgta
     8281 ttgggtagca taaagaatca atttcatatt ttctttccca gatccaatcg ttcatttttg
     8341 tatgatctgt ttggtggcca gcaccgttag cttcttcgtt gaaggcattc gcatatggat
     8401 cgatattgat ataaaaaaat tgctttttga ctaacccgct gatcatgtca gctaattcct
     8461 catcttcttt tgcaatcact aaataaggac gaacttgtgc agtagagtct cttaaccaca
     8521 tcgctggaat gtctcccgtc aataaaaagg tggttccgtc attttgcttt ttgactgttg
     8581 tcaacagagt attggcaaat gctgctgtaa aattctttgc ccagtccgca tggatatccc
     8641 cacatttttg tgtgatatct tccataaatt tctgcaccga ttttggtata tctttataag
     8701 ccactaataa aaccttcttt ctatttgata tatctttaaa gattatactt catacagcca
     8761 attatgtcta cgctttcaaa aaatatcttt ttatttactt tttcatccat tatggtatta
     8821 tttgaatatg atatatcatt aagaggtgtt tatatgaatc aaccattgta ccagacaata
     8881 tttaatgatt tgcaaacggc tattttaact ggtgaattac ctgtagacag ccaactacca
     8941 actgaaaaag aactttcacg tacctataaa gtaagcagaa tcacttccaa acgtgcattg
     9001 actgaattag agcaattagg acttatttat cgcgttcgcg ggaaaggcag tttcgttaaa
     9061 tccgcttctc cggaacagat tcttgcttct cctacaaaaa cacgacggat tctgtttctt
     9121 cttccatatg tcgctgattt gtctgttggt gattttagca agggattgaa ccctgtgatg
     9181 caagataaac agattgatgt catgatgacg acgcttgact ttctacaaaa taaaacagca
     9241 aaagagctaa tcaatgaatt tgacggattg atttattatg ctcttgatac agaacaacat
     9301 cttgacttac tttttgagct ttcattgaaa gaattccctg tcgtcatatt agataaaaaa
     9361 atctatgagt taccttttcc taccgttctt tccgataatt tccaaggcgg tgcgcttgct
     9421 gcctcttatt tgatcgaaca gggacattca aaaatcgctt atttgtttgg tacgcagact
     9481 catccacaat ccgttcgaca gcgttacctt ggttaccttg aagcattgga caaagctggt
     9541 ttgactttcc acacttcact ggatgacaaa agcgcgacat tagatgaatt gctttcttat
     9601 atcactaagt atgaagtaac tgcttttgtt tgtgaaaacg atgttgtcgc tatccagacc
     9661 atgaatctct tgagacagca tggttatcat atccctgaag atttttcaat tatcggattt
     9721 gatgatatcc aagcagcagc tcttgttgac ccgccactga caaccatcgc acaagatttt
     9781 gaacagttag gtaagcttgc gggagatgct ttggtcactt ggttagagac aaaagatcga
     9841 ccatccgata gtaaatttcc agtttcattc atcaaaagac aatcaacaaa ggagaaataa
     9901 aatgaatatc ggtcaaatag atactagaca tgcaacagcg aatcagccaa atttctctaa
     9961 tgggaactgc cagccttata ctggcgttcc ttttgggatg aattattttg caccgcaaac
    10021 tacggatcaa aacggcagct ggtggtttca tcctgatgac cgcgtttttc aaggctaccg
    10081 tcttacccat caaccaagtc cttggatggg tgatttcagt catatgctgt ttacgccgat
    10141 caatggaaaa ctacaggaga acaccctttt ccatgctcaa agttcttacc gcccagaaga
    10201 aagtatattt tgcccgactc atctgtcgat ctgtcaatta cgttatggca tctgttctac
    10261 attgatacct agcatgtatg gcggaatctt atctattgat tacagcaaaa atgacagcgg
    10321 tttactttta tcctttccgg gacgccatca gctctttgtt atagatcctt acacagtgga
    10381 aggaaaaatc acacattttg ctggttcaga agatcctgat ttttcttttt attttatatt
    10441 gagatttgaa tatcctttaa caattgatga gttagctgtt tccaacaaaa atagcgactc
    10501 gatcccgttg tattttgaac agacaaaaaa acaaactatc cgatttggga catcttttat
    10561 cagctcggaa caagcagctt ataacctgga actagaacaa aaccaaagca aagagactta
    10621 ccttgctcac agtcaagaac aatggcagtc ctattttgat cgtatacagc tcacacacca
    10681 tgatcaacaa caattgcgga cgttttatca taatttatat cgggtgtttt tatttccaca
    10741 aacattttat gaaattgatc ctagtgggca aaaaatccac tatgatacga ctagtcgatc
    10801 tataaaacct ggtgttctct atacaaataa tgggttttgg gatacgtata aaacggttta
    10861 tcctctttat tctttgattg ctgtagaaaa atacgaagaa atgctggaag gttttttgaa
    10921 cagctataga gaaagtggct atttgccaaa atggctgtct cctgatgaac gtggacttat
    10981 gcctgggaca ttgatcgatg cggtcattgc agatgctgct gtaaaagata tccgacgaga
    11041 cttgatgcct gaatttctaa cggcaatgga aaaaggtgct acggttcaaa gcaaacatcc
    11101 aaattatgga cgccaaggaa cagaagatta cttgacatat ggctatgttc ctaatcacta
    11161 ccacgaatca gtcaatcaca cattagacta tgcttatagc gatttttgta ttagccaagt
    11221 tgccaaggta ctagaagaag aggaaatgac gaagaaatat cagcagcaat cattgaacta
    11281 tcaaaatatc tttgactatg agtcaggatt tatgcgttca aaagataaag atggcaagtt
    11341 cagagaacca ttttcagcta atcgttgggg acaagattat gcagaaggaa gtgcttggca
    11401 aagcagcttt gctgtttatc acgatttttc aggactgatc ggaaaatatg gtggcagtga
    11461 gagatataaa gaaaaactca ttgagctgtg caatcagccc ccaacgtttg acccaggagg
    11521 atatggtttt gagatccacg aaatgagtga gatggccgcg atagaatttg gacaattagc
    11581 tatttcaaac cagccaagtt ttcactatcc ttacttgttc agttatatag gacaacctga
    11641 aatggcacag ccactgctta aacagttgat gactcagacc ttcgacgatt ctccaatggg
    11701 ttaccctgga gatgaggaca atggaagtat ggccgcttgg tatatcttca atagtctggg
    11761 attttatcct gtcacgcctg gaaccggtga atatgttatc ggtatgccat taattgacaa
    11821 ggctgttttg cttttgtcaa atggaaagga attgacgatt gaagcaacgc ctaatcggcc
    11881 gcagcaacaa tttatccata aaatcaaacg gatggacaaa gaatatacga aattatattt
    11941 tacccatcaa gatttgctga aaggcggcac gatctcttac caattaggga tcatccctca
    12001 ccctaagaaa tatcaagcaa aagatttgcc tttttcctta tcaaactata attaa
//
LOCUS       DACENJ010000073        11974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.073, whole genome shotgun sequence.
ACCESSION   DACENJ010000073 DACENJ010000000
VERSION     DACENJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.073"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..498)
                     /locus_tag="IW748_001425"
     CDS             complement(<1..498)
                     /locus_tag="IW748_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104342.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIF"
     gene            complement(598..1107)
                     /locus_tag="IW748_001426"
     CDS             complement(598..1107)
                     /locus_tag="IW748_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ1104343.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1192..1671)
                     /gene="rlmH"
                     /locus_tag="IW748_001427"
     CDS             complement(1192..1671)
                     /gene="rlmH"
                     /locus_tag="IW748_001427"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1104344.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2099..3388
                     /locus_tag="IW748_001428"
     CDS             2099..3388
                     /locus_tag="IW748_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1104345.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENIKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(3427..4032)
                     /locus_tag="IW748_001429"
     CDS             complement(3427..4032)
                     /locus_tag="IW748_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1104346.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4237..4701)
                     /locus_tag="IW748_001430"
     CDS             complement(4237..4701)
                     /locus_tag="IW748_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1104347.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4723..5043)
                     /locus_tag="IW748_001431"
     CDS             complement(4723..5043)
                     /locus_tag="IW748_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1104348.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5187..6266)
                     /gene="pepA"
                     /locus_tag="IW748_001432"
     CDS             complement(5187..6266)
                     /gene="pepA"
                     /locus_tag="IW748_001432"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1104349.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            6466..6816
                     /locus_tag="IW748_001433"
     CDS             6466..6816
                     /locus_tag="IW748_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1104350.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7039..7986
                     /locus_tag="IW748_001434"
     CDS             7039..7986
                     /locus_tag="IW748_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1104351.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8043..9461)
                     /gene="pepV"
                     /locus_tag="IW748_001435"
     CDS             complement(8043..9461)
                     /gene="pepV"
                     /locus_tag="IW748_001435"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1104352.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            9610..10455
                     /locus_tag="IW748_001436"
     CDS             9610..10455
                     /locus_tag="IW748_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1104353.1"
                     /translation="MLINEQLVKRIITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            10459..10899
                     /locus_tag="IW748_001437"
     CDS             10459..10899
                     /locus_tag="IW748_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1104354.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARIALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            10949..11143
                     /locus_tag="IW748_001438"
     CDS             10949..11143
                     /locus_tag="IW748_001438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104355.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            11279..11965
                     /locus_tag="IW748_001439"
     CDS             11279..11965
                     /locus_tag="IW748_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1104356.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
BASE COUNT         3581 a         2380 c         2193 g         3820 t
ORIGIN      
        1 aaatatttca ttgccatatt ccgtccttaa aattatattt tcagtgccag acaagtgatt
       61 tgtattgagt tgataaaaaa gatctccatc taaaaaaata tcattagaag aagatttccc
      121 cacagtaatg accattccat cagctctatg tgttacatac aaataattgt atgacctcat
      181 ttcacctaat gaatcaatta tttcttgccc tttagctcta atatattgat ctctattacc
      241 tatctcactc ttatttaaca aaagtttttt catgtcaaat ttaatcggct gttttatttt
      301 tgtaaattcg cttctactac taatccaatc cgagattaat ttttcaacat gtcccagagt
      361 aaactctgat ttattcgcta aatacttttt ttgttgtatt aaagacatct ttccccgaag
      421 ctcaaaattg agcctacatc gattctctat ctcttcaaaa agcatcttat cttcatcatt
      481 cttatattca tcattcataa aaccaatcca gctcctcgat taagataata ttttaatctc
      541 atacaaaaac tagctctcat caaaacaacc attgttttga tttctcatga aatttcttta
      601 tacttttctc cgattataaa ttcctaaccc cttgagaaaa ttacgcatat atttatctcc
      661 gcaaggctta aaatttcgtt ggccttcttt tctcaaaaat gccccgattt cgccttttga
      721 tggatagatt tctgcctcag caaaaacgtc taaaatatca tcagtggtca tagacaaggc
      781 gatttttaat ttttttagca acacattgtt gatatatttg atttccttgc ttttttgcgg
      841 cgtgtctact atcggcttat ctttttgcgg accacgcgcc aagagaatca ggccatttag
      901 gaatgcgtca aaaacttggt tgttgatggt tttttcatac acattatttt ctgcattatc
      961 tttgttctta tcctgtactt tggtcagtac cgcctgagct tcttctttag tcagtggtac
     1021 accgcctagc tcaaaagctt taatcaaatc cgtatctttc aaatcaatcg cataacgcaa
     1081 tcgcaataac ctatcattgt tattcatttg ttcctccgaa gtatttagtc tttactttct
     1141 tacgtacttt ttgtcttgct aagattttta ggatcaatga taaaaaccgc atcacttatg
     1201 ataaggcgcc cccgcattga tccggaaggc acggtagatt tgctcgacaa ggatcagacg
     1261 catcaattga tgtggatacg tcatttttcc aaaagagatc tgagcattgc ttcttttcat
     1321 gacggcttcg ctgagtccta aagagccgcc gataacaaaa acaaggtggc tttttccttg
     1381 gatacctaat tggtcgattt gtttagcaaa agattcactt gtaggatttt tcccttcaat
     1441 tgctaaggca tagacgtatt cttgttcttt gattttcgct aagatccgtt ccccttcttt
     1501 ttcttttact tgtctcattt gcgcttcgct taaattctcc ggggcttttt cgtcaggaac
     1561 ttcgatcaat tcgatttttg cataggcatt cagccgtttg acatactcat tgataccttg
     1621 gatcaggtat ttttctttta attttcctac tgagataatt ttgatattca tgttttctct
     1681 cctttggtct tcatgctcac tttgtttatt ataatcaatt ttttcgtcta tatgtttatt
     1741 ttggctattt ttgttaaaaa gatttcccca acagttatgc acaacatcca caggtttatc
     1801 cacagaaaac agtgttattt taccttattt catcacattc attaggcaaa catacgtttt
     1861 ataagaattt atccacttta tacacaggct gtggatatgt tttatccctt atttttccaa
     1921 tcctatccgc gtagttttac acagtatctg tggaaaaaga aaatcaaata ttttcatctg
     1981 aaaacaatga tttttcctaa agtttttcat aaaaagttca cagtctcaaa gtagactaaa
     2041 gtcaacaaat aagactatac taacagtaga taaatgaatg taattggagg aggaacacat
     2101 ggatagaaaa aatgtgacac caaagatgaa aaaaagtaaa aataatagcc tctggcgtaa
     2161 gttaggtctg gggcttgttg gagggatcgt tggaggacta ctgacagctg gcatttttta
     2221 tgcagcgatg ggcactggca acaatagttc tactgctaca agcggcaatc agaattctgc
     2281 cggtgaaaca gttgtggaaa atatcaaagt caatgtagat tctgatatca ccaaagcggt
     2341 agataaagtt caagacgctg tcgtttctgt tatcaactta caaagtcaaa accaatcaag
     2401 cggttttggc ggactattcg ggcagcaaga agaaagtagc agctcagatg atagtaattt
     2461 ggaagcttat agtgaaggaa gcggcgtgat ctacaaaaaa gacgggaata ctgcttatgt
     2521 agtcacgaat aatcacgtag tagatggcca gcaaggtctt gaagtcttga tgaaagacgg
     2581 tacgaaggta aaagctgaat tagttggaac agatgcttat tcagatttag cggtgttgaa
     2641 gatcaattca gacaaagtcg aaacagttgc ctcttttggt gactcaagtg ctctgaaggt
     2701 tggtgagcct gcgattgcga ttggttcccc actaggatct gaatatgcaa attctgtcac
     2761 ttcaggaatc atttcttcct tgaaccgaca agtgacaagt acgaatgaat cgaacgaaac
     2821 agtcaatatc aatgcgatcc aaacagatgc agcaatcaac cctggaaatt ctggtggtcc
     2881 tttagttaat atcgaaggac aagttatcgg tatcaactca agtaagatcg caagtacttc
     2941 tgaatctact tctaatgtaa gcgtagaagg aatgggattt gctattccaa gtaacgatgt
     3001 agtgaatatc atcaatcaac tggaaaaaga cggaaaagtg acacgtccag ctctaggtat
     3061 cacaatggtc gacttatctg ccgtttctac tcagcaacaa gaacaaatct tgaagattcc
     3121 ttcatctgta actaacggtg tcatcgtgac aaatgtccaa acagctacac cggctgaaaa
     3181 agctggattg aaacaatacg acgtcattac taagatcgat gacaaagaag tttcttctgg
     3241 tgtagaactt caatctgctt tgtataagaa aaaagtggga gattctgtca aaatcactta
     3301 ctatgaaggc tcgaaagaaa aaacaactac tgttgaattg acgatcgatc aatcggcatt
     3361 aaaacaaaat caaaatagtg aaaattaata aaaaagaggt tgtgacaaag acttgtcaca
     3421 acctctttat tttggaaatg cttctccgac tacggcatcc tcaggcaata ctaagattcc
     3481 tttttttgct ggagcatttg gcaaatgcaa ttcacgagcg gaacagatca ttccatagct
     3541 atctacccct cgcaaagttc ctggccagat catcattcca tctggcatca tagctcctgg
     3601 ttttgcaacg acaacttttt gtccagcttc gatattgggt gcaccgcaaa cgatttgcaa
     3661 tactgtacca ttgtctactt ctgtctcagt aatcgataaa tggtcagaat caggatgtgg
     3721 gacgcatgtt tttacgtacc cgacaacgat cttcggttca tgatcagctt caagctgttc
     3781 ttcgaatcct gcttctttga tcaatcgatt caatttcgct aattcctcat ctgttaagct
     3841 tacttggccg ttcccttcga ttgttagatg atcggagata tggaaaaagt tccaagcaac
     3901 aacttgacct tgttcatctt tgatccgagc aacattccat ttgcgttcaa cagtgttttc
     3961 tgttccttta tcgtcggcta taataaccat caaggtgtct ccgacgtgtg ctttgttata
     4021 agcaaaaatc atgttctttc ctccattatc ctttgctgag atttatctta acaaaaaatt
     4081 gcattcactg catgaaattt ttttcatgct ttatttccga agaagcctct tctggaacac
     4141 catttattcg taacaaggga ctgtgacaag acttttgtca cagtcccttt actgttttag
     4201 ttaattgatt aaaaaggtcg tacaagttcc acttgcttaa ttacttggct gttcttcttc
     4261 gtcagaaacg atcaagacat cacaaggtga atgacgtatc acgtagctgg ctacacttcc
     4321 tgtcataaag cgttcgactg catttagacc tgactgccca accatgatta gatcgatctc
     4381 atattttttt ggcaatttga ctgccatgga ttcacgaaca gaaccatatg caataattcc
     4441 ttctatattt gtgtaatcaa cagatttccc atattcttta cattcttcga tcatttcctt
     4501 ggctgtttca gtttgctgat cgactagatt ttgatcaata ggtgcatagc ccataaagtt
     4561 ataaaattgc tgatcgatta cgtttgctac atatactttc ccattattac ggcgagccac
     4621 ttctaccgct tttttgaaag cagcaagtgc ttgttcacta ccgtcgatac ctactaaaat
     4681 attcttatat tcttgattca tcttgacccc tcctaacaga tgtcagcctt ttgttacact
     4741 ttctaggaac gattcgattt cctgttttgt tttacgatct ttattcacta gacgacctaa
     4801 ttcttttccg tcttggatca cgacaaaact tgggatacca aaaatgttcc attcagcggc
     4861 tacatctatg tacttgtcac ggtctacttc aataaattgc caatcagaaa agtcattttc
     4921 aatttcaggc atttgcggtt tgataaaacg gcagtctccg caccagccgg cagtaaagaa
     4981 aaatacactc tttccagttt ctacgtagcc tgctaattct tctaaactat ttgggataat
     5041 cattctttgt cctctacttt ctgtttatgt atttttcaat taaattatac acctttcttt
     5101 cgaactaaca aacattgatc ctctttttat aaaaaaaatc tggcaaaaag caatcttttg
     5161 ccagatttta tagtttcact atcgcattat tttccgtata cgatcgtatt cactgttgat
     5221 ttatccaaac ctttcagtgt ttgaatcaac atttctcgtg cagcttcaaa atcgcgaata
     5281 ctgaacatcg tttgatgcgt atggatataa cgtcccacta cgccaatcac tgtacttggg
     5341 attccttcgt tttgtgtatg agcagcacct gcatctgtac cgccttttga aacaaagtat
     5401 tgataaggga tattgtttgt ttcagcgata tccagcaagt actcgcgcaa acgaggaagc
     5461 atgatcaaac ctggatcttg gatacgcatc aatgtacctt ctcctaaatg tccgaatgtg
     5521 ccatttttcg tcactgtatc atctgctgca gagcagtcta ctgcaaagaa cagatctggc
     5581 ttgaatttat tgacagaagc tttagaacca cgcaagccta cttcttcctg gacattcgcg
     5641 cctgcaatca atgtatgacc taatgtttca ttttgcaacg cttctaaagc ttctaatacc
     5701 attgtacagc catagcggtt gtcccatgat ttactgataa tattctttcc gttggctgtt
     5761 ttgatcgttt cagcgtaagg aacgattgtg tctcctggaa ggacaccgta actcattgcc
     5821 tcttctcttg attcaaaccc agcatcgaac aaaatatcgg ttacttctac tgatttttgt
     5881 ccgcttgttc ctcgcaataa atgtggggga acggaagaag aaatacatgg ataattctta
     5941 ccagtagagg ttttaagtgt aaaacgctga gcagaaacaa cgtaaggatt ccagccgcct
     6001 agaggtacga ctttaaaaag cccgttttct tggatttgtg tcagcatgaa tccgacttca
     6061 tccatatgtg ctgcgaccat tacacggggt gcatccgctt cttcatgatg gcgcagacca
     6121 aaaatgccgc ccaaaccatc taactgtaac tcatctacca gcggttccat atgactttcc
     6181 atataagcgc gaacatcttg ttcaaaaccg cttgttcctt gtaattctgt taactctttg
     6241 attcgttgaa atgttttttc ttccatatgc tattcgtcta aatttcagac taaactcctc
     6301 tcagacttat attgatctgc ggataaaata tgattttttc tgcagaccga tcgtatttat
     6361 tatagcttat tttttctggt agcggttatt tatttcaaag aaaatatgag agtgtggtaa
     6421 aataagcaca aggagtaaca agtgagaaga aggaggtatg tccacatgta tgaagaaagc
     6481 gaactaagct attttaaagg cgggttagca gttggaatcg gtctaggatt attgagcggg
     6541 gtagcttcaa cgatttggta tcatcgcaaa aaagtattgt cagcagacca agtacttgaa
     6601 aatatcaagt catcttttct aaaagaaggt gcaatcgaag gttcttggat ctcttttgaa
     6661 aaggaaccta ttaggaaatt cgccgttcgg tcgagtgctt atcggggagg tctttctcga
     6721 atagaagatg gcgaaactgt ttattatgaa tttttagcag atgctcatac aggaaccgta
     6781 ttagaaattt caagaaaaaa aggtgcaaca gattaatacg tcaaaaaagt gagagaacga
     6841 atacgcaccc actattcaaa agagaggtat catgtccttc ttttgtagtg ggtggttttt
     6901 tagatagagc tttccaaaca tatattccag tattttcttt ttaaataaat gatatttagc
     6961 gggaaaaact agcatttcct ttgattatga ggtattttta atttagcttt tgttttctat
     7021 agttaaggag agtgaaaaat ggaacaacaa gaaaaaattg ctgttctgat tccttgttac
     7081 aatgaggaag caacaatttc aacagtcatt gcagacttta aacgagaatt accagaagca
     7141 gatatttatg tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggt
     7201 ggagcaatcg taaaaaaaga acctcgtcaa ggaaaaggaa atgtgatccg ccaaatgttc
     7261 tttgatatcg atgccgacta ttatttaatg gtagatggcg atgatactta tcctgcagaa
     7321 gctgtacatg gtttgctcga gaaattacgt tctggtgaag cagatatggt cattggtgat
     7381 cgcctatcaa atggaaccta ctttgatgag aataagagac ctttccacga ttttggaaat
     7441 aaccttgtta gaaatacaat tactagaatg tataaaacaa aaattcttga tgtcatgacc
     7501 ggctatcgag gattcaatcg tatctttgtc aaaagttttc ctattatgag ttctggtttt
     7561 caaatcgaaa cggaattgac gatccatgct ttagataaaa aattcaagtt cgttgaaatc
     7621 ccaatcgatt atcgtgatcg tcctgaaggc agcgaatcaa aattgaatac attctctgac
     7681 ggattcaaag tgatcatgat gattattaaa atgtttaaag attataagcc tttgcttttc
     7741 tttgggattt ggaccatctt cttcttttta tttggattgg tcacggggat tcctgtgatt
     7801 cgcgaatata tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga
     7861 ttgatgactt tagcacttct ttcattagta acagggttga ttttggatac agtcgtcacg
     7921 aacgctaaga aagaatatga attgaatcta tatcatgttt atcatgaata ccacagtaga
     7981 aagtaaaaaa aggatcaaga ctgcaaaaat aatttgcaat tcttgatcct ctttttaatt
     8041 aattatttcg ccaaacgata gatggcatct gcatagattg ctgttgctct gaacaagtca
     8101 tcaagactca taaattcgtt tgcttgatgc attgtatcag tatatcctgg gaacatcgct
     8161 ccataagcaa cgccacgttt caacaaacgt ccgtaagttc cgccaccgat gatttgttcg
     8221 tagccttttt ctccagtgtg gtcttcatag acttgcaata atgtagccac caatggatca
     8281 tccattggta cgtaatgtgg ttccattacg cgagcacctc gagtaactgt tgcgccttct
     8341 tgccccactg tttgttcgat gtcactttgc aatccgtcta ctgttacacc ttttggaaaa
     8401 cggaaattca tattgatgaa gttcgcttcc tctgtaccat tttcttcaaa agcaaatagt
     8461 ccagcattca ttgtcaattt gcccattttt tcatcttcat aagcgactcc tagtttttct
     8521 ccataaaaat cttcatggac ataagcagct gctacatgga taaattgttt agcagatcct
     8581 aggaattcat agttatctaa gaacaatgcc aagaaagaac ctgcattgat accagattgt
     8641 gggcttgcac catgtgcgcc tttaccaact aattcgattt ttacatacgt attgtccgct
     8701 tcaatcgttc cgcttactgg atttgcttcg atgaattggt aaaatgcttc ttccatcgcg
     8761 atagctgcat ctgcacttgg tacttgaagt gctgcagttg ctgttcctgg caccatattt
     8821 tcgcgaagtc cggaaacaaa agatttcaag acataatctc ctgcattgtc gcctttgaag
     8881 cgtaaagcta aagaaacatt tcctttttca ccattgatga ttgggaattc agcatctggt
     8941 gagaaaccaa aatctggttc ttcttcatgt tcgaaataat aagccatatc gccccagccg
     9001 ctttcttcat cgctgccgac tacgaagcga actttttttg atagtttcaa gtcaagttct
     9061 ttaatgatct tcatcgcata gtaagctgcc atacttggac ctttgtcatc gcttgatccg
     9121 cgtgcaaaga tctttccatc cttgattact ggttcataag gatctgtgtc ccagccatct
     9181 cctgcaggaa cgacatccat gtgtccaaat atccctagcg tttcgtctcc ttcgccaaga
     9241 tcaatatgcc ctgcatagtt atcgacattt tttactttaa atccatcacg ttcgccataa
     9301 gctaacatat gtttcaatgc gtcgcgaggt ccaggtccaa atggtgcatc tggtgttact
     9361 tttgagtcat cgcgctcgct gttgacacgt aaaagatttt tcaagtcttc aagcaagtct
     9421 tctttacgtg cttctacttc tttttgccaa tcaattgtca taaagttaat cctccactta
     9481 tttaagtctt cgtgttcatg ataccacaaa ttacagaaaa aaatagttca aaaaatctga
     9541 caattccttt tcttatctgt ttttctctaa aaaaaatata cgctataatg agtaaaagga
     9601 ggtttttaga tgctgataaa cgaacaacta gtgaaaagaa tcatcacacc tcgtccaaaa
     9661 atttcacata aaggaacatt tggacgtatc gttttgattg gcggcaatga acaatatggc
     9721 ggagcaatca ttatgagtgc tttagctgcc gtacacagcg gagcgggtct cacgactgta
     9781 gtaactgatg aaaaaaatcg gagtgctctt cacagtcatc tgccagaagc aatgattgta
     9841 gtttggacgg atactgacca gtttctttcc gtaataaagc aagcagatgt actcttgatt
     9901 ggtcccggat tgggtacttc ttctaagagt ttggaacgtt tcaaacaagt actagaaaac
     9961 caatcaaaag aacaatggct ggtgattgat ggctctgctt taagtttact tgctgctaat
    10021 catcttttgc taccctatcc cgaacaaact gtacttacac ctcatcaaat ggagtggcaa
    10081 cggttgagtt ctctaccgat acaagaacaa cgggataaag aaaacaaaaa gaagcaacag
    10141 gaaatcggtg ctgtgctcgt tttgaaaagt catgagacaa aaatttatgg tcaccagcac
    10201 ttctacacca atcctttagg ttctgctgct atggcaacgg gcggcatggg tgacacttta
    10261 gctgggatga tcacaggatt tttagcccag tttcctcaaa atgaccaaac gattgcagca
    10321 gctatttatc ttcatagttt cattggagac cagctggcaa aagaacaata tattgttttg
    10381 ccgacacaaa tcagcgaagc acttccgaaa tggatgaatt atttttgtaa aaaatcaatg
    10441 gaaaacgagg catgagtcat ggagaaattt acaggttatg tgcgagaacc tttttattat
    10501 gaaacagatc aaatggggat tattcatcac agcaattata tccgctggtt tgaagaggct
    10561 cggatagcct tattggtaca tctagggttt ccctataaaa aaatcgaaga atctgggatc
    10621 atcatccccg ttctggaagt aaattgcagc tataaagaaa tgatccatta cggagaaaaa
    10681 gtgagcatcc atccaactat ccaaaagtat acggggacac ggttggattt tagctacgaa
    10741 attgtgggaa ctgaagacaa aaaattaaaa actactggca gtagtaaaca ctgtttttta
    10801 ctttctgatt cgcaacgatt agtgaagctg agcaaagtca atccggagtt agatcagtta
    10861 ttccaagcgt acgcttcttt ggatgatgta aattcttaat aaagaccgtc taagcatttt
    10921 tcttgtattg tgtcgagtag ttcgtataat gaaaagaaaa aagcaggtga tccccatgta
    10981 tgtcgttaaa gtaatgcacg gttacatcga taaaactggc tgtcgcactc gggaaaaaaa
    11041 cttagacaat ctgctcattt tcaaagacaa aaaagaatcc gaagcttttg ctaaaagaat
    11101 cggcggtcgt gtcaaaccga tacaagaggt acgaccagat tgattcaata tttttctttt
    11161 cgaggctcta ataggcatgc ctatcagggc tttttttgca gactgtgttt ttatgcccac
    11221 acgatttgta agcgatttat gttataatag attatatctg catagaaagg aaggttaaat
    11281 ggttatcaat aatttgcaac agcaagctta tgaatctata cgtaaacaaa tcatttattc
    11341 cgagttagca cctggaagta aaatctccga taaagtgtta gaagaacaac tagctatcgg
    11401 gcgtacaccg attcgagaag cactgatcca actgcgcaat caagagcttg taaaaaccat
    11461 tccacagtcc ggcacttatg tttcaaaaat cgatatccga tctgcttcat tagcccgtta
    11521 tacgcgggaa aagttggaaa aaccgatcct gcaagaatgc agtgcgaaaa tgaccaagca
    11581 agatcaagct aaactagaaa aaatactaga acaaaccgat caggcagtaa tcgctcaaaa
    11641 taaaaaagct ttttttgatt tggatactga ttttcaccga acttgttatg aaattgctgg
    11701 aaaacgagaa atttgggatt ggctagaaag ttatagtacg cacttgaacc gtttcagatg
    11761 gttacgtttg acgatttcag aattagactg gggtcgagta ttagatgaac atcagacaat
    11821 gcttcaatca atgattgatc ataactttga tgaagtcgga tttctttgca cgatgcattt
    11881 acatatgatt atcgaggagc aagaatacgt gattcataat taccccgatt attttgaaga
    11941 tattcaggac agacccatca aataagaggt tttg
//
LOCUS       DACENJ010000074        11874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.074, whole genome shotgun sequence.
ACCESSION   DACENJ010000074 DACENJ010000000
VERSION     DACENJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.074"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            129..743
                     /gene="gmk"
                     /locus_tag="IW748_001440"
     CDS             129..743
                     /gene="gmk"
                     /locus_tag="IW748_001440"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1104357.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            748..1053
                     /locus_tag="IW748_001441"
     CDS             748..1053
                     /locus_tag="IW748_001441"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1104358.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1306..3714
                     /gene="priA"
                     /locus_tag="IW748_001442"
     CDS             1306..3714
                     /gene="priA"
                     /locus_tag="IW748_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1104359.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3729..4220
                     /gene="def"
                     /locus_tag="IW748_001443"
     CDS             3729..4220
                     /gene="def"
                     /locus_tag="IW748_001443"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1104360.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4213..5151
                     /locus_tag="IW748_001444"
     CDS             4213..5151
                     /locus_tag="IW748_001444"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1104361.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5151..6509
                     /gene="rsmB"
                     /locus_tag="IW748_001445"
     CDS             5151..6509
                     /gene="rsmB"
                     /locus_tag="IW748_001445"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1104362.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6522..7262
                     /locus_tag="IW748_001446"
     CDS             6522..7262
                     /locus_tag="IW748_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1104363.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7259..9328
                     /gene="pknB"
                     /locus_tag="IW748_001447"
     CDS             7259..9328
                     /gene="pknB"
                     /locus_tag="IW748_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1104364.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9493..10392
                     /gene="rsgA"
                     /locus_tag="IW748_001448"
     CDS             9493..10392
                     /gene="rsgA"
                     /locus_tag="IW748_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1104365.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10405..11055
                     /locus_tag="IW748_001449"
     CDS             10405..11055
                     /locus_tag="IW748_001449"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1104366.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11056..11694
                     /locus_tag="IW748_001450"
     CDS             11056..11694
                     /locus_tag="IW748_001450"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1104367.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
BASE COUNT         3974 a         2209 c         2631 g         3060 t
ORIGIN      
        1 acgacctctt ggtacagggt agctttttta ccaatttcta gctataatta aagataacct
       61 tgaaagttgc agaaaaaaag tgcaaaataa acgataagta caaaaagact agttgaaagg
      121 acgtcgtcat gtcagagcgt ggattattaa tcgtgttgtc tggtccatcc ggtgtaggaa
      181 aaggcacagt acgaaaagca atttttgaga gtgaggataa tgattttcag tattcgatct
      241 caatgactac ccgtaaaatg cgtgaagggg aggtcgaagg cgttgattat ttcttccgaa
      301 gcaaagaaga gtttgaatca atgatcgaag ctggagagat gctggaatac gcagaatatg
      361 tcgggaatta ttatggaact cctttgtcat atgtcaacaa gacattggat gaaggaaaag
      421 atgttttctt ggagattgaa gtccaaggag caaaacaagt caaagaaaaa gtgcccgatg
      481 gtgtattcat ctttttgact ccgccagatt tagcggaatt aaaatcacgg atcgtaggcc
      541 gaggaacaga tgcagatgaa gtaatcgaag aacgaatgaa agttgcaaaa gaagaaatcg
      601 aaatgatggc tctttacgac tatgctgttg taaatgatga agtgccgcta gcagttgaac
      661 gaatcaaaaa cattattgcc agtgaacatt ttcgagtaga tcgcgtaatc ggtaaatata
      721 ttaaaatgtt aaaggagatg tagtcttatg atgttaaaac cttcaattga ctcactatta
      781 gatcgtgtaa attcaaaata ttctctagtg attttagcaa gtaaacgcgc gcatgaattg
      841 gatgcaagtg cacagcctac tctagattct tttgattcag taaaaagcgt cggtcaagct
      901 ttagaagaaa tcgatgcagg aaatgtagtc aatgatccac atcctgaatt aaaacgtgaa
      961 cgtttgaaga tggaagaaga ggaacgtcaa gcacaaaaag agcgtgaaca acacgaattg
     1021 gaatcacgta tccgtgaaga gcaaaaaatg taataaagaa aaatagagcg aacatctaaa
     1081 aagaaaagca aacccagctg tttccttttc aaacattcgc cacttattgt ctggtagaaa
     1141 aggctgtgtc tgaacttttt gacacagttc ttttttttct tatctgctgt taattttttg
     1201 ttaggatttg gcagaagata agacaaatcc atcgatctct agtaaaatag gcaaggaact
     1261 ttttgtcgct tgataagaaa agacaaaaaa aggaggaaac gaaccatgac attaacagct
     1321 gaagtcattg tggacgtacc tactatgcag accgatcagc cattcactta tttggttccc
     1381 tctgaaatgg aaacagcttt gcaagtcggg atgcgagtag aagtcccttt tggtaatggc
     1441 aatcgtcatg tccaaggatt tgttatggct atccaaaaat cagaagaatc agctaatcca
     1501 tctttgaagg cagttattcg tttgctagac cttgctccgg tggtcaatga agaattactt
     1561 tcattagctg attatatgaa aaaaattact tacgcattca aaatcacttg tctacaaacg
     1621 atgcttccga gtgtgatgaa agcagaatat gataaattga tctatccttt ggcagacaca
     1681 ccagaagttc aggtattgtt tgatcagcga gaagtgattt cctggaagga agcagaagaa
     1741 gaaggcagcc tttctcaatt gattcgctgg cgccaagagc aattagtaga tatcaaatat
     1801 gaagtccata cacgaaataa agtaaaaacg attcgtttgg tccgttcttt gttaactgaa
     1861 aagcaaatcg aagaagaatg ggcaaaatta cggcaaaatg ccaagaaaca aaaagaactt
     1921 cttttgtgtt tatctgaact gagccaagaa gaaccaattg cttatttcaa aaataaagga
     1981 atcagtactg ctgtcttgaa tcaaggaaaa gaaaagggct ggctggagtt tatagaatca
     2041 gaacgttatc gcgaccctta taaagaccga gtatttgacc aaacgacggc attggaactg
     2101 aatgcagaac aaaaaaatgc tgtcgaacaa atcatcacag caggtcagca gcaaaaagat
     2161 caggtctttt tgctggaggg aatcactggc agcgggaaaa cagaagtata tcttcaggca
     2221 atcgctgatg ttttgtcaga aaataaaacg gctattatgt tagtgcctga aatcgcattg
     2281 actccgcaaa tggtcgagcg attcaaagga cgcttcggag aatcagttgc ggtcttacat
     2341 agcggacttt cccaaggtga aaaatatgat gaatggcgca agattgagcg ggaagaagcg
     2401 caagtagtag taggcgcacg atctgctatt tttgccccgc tgaaaaatat cgggctgatc
     2461 attatcgatg aagaacatga atcaagttac aaacaagatg agacaccgcg ttatcatgcg
     2521 agagatctag caatttggcg cagtaaatat catcattgcc caatagttct aggaagcgcg
     2581 acgccctctt tagaatcaag agcacgagct caaaaaggcg tttatcagtt attgcagtta
     2641 aaccaccgag ccaaagcagc tgcacagctt ccagctattg agatcgtaga tatgcgtgaa
     2701 gagtttcaga atcatcggac ctctactttt tctgctaatt tgcaggaaaa aatacaaaat
     2761 cgattagaca aaaaagaaca aactgtccta ctgctcaatc gtcgaggata ttcgtctttt
     2821 gtgatgtgta gggactgtgg atttgttctt ccatgtccta attgcgatat ctctcttaca
     2881 ctgcatatgg atacacgttc aatgcgctgc cattattgcg gacatgaaga acctattccc
     2941 aatcgctgtc ctaattgtgg cggcaataaa atccgctact atggaacagg tactcaaaaa
     3001 gtagaagagg aactccgaga actttatccg caagctagga ttttgcgtat ggatgtagat
     3061 accactcgaa gaaaaggggc acacgaacag attttacaaa aattcggtgc aaaagaagca
     3121 gatatattat tagggacaca aatgattgct aaaggtttgg actttccaga agtgacatta
     3181 gttggggtgt tgaatgctga tacctccctg aatttaccgg attttcgttc tagtgagcat
     3241 acattccaat tgcttaccca agtttcagga cgagcagggc gagcggaaaa agcaggcgaa
     3301 gtcgtgatcc aaacattcaa tcctcaacat tacgccatag aattggctaa aaaacaaaat
     3361 tatgagcagt tttatcagca ggaaatgcat gtacggcatc gtggaggcta tcctccttat
     3421 tactttactg tgaaaataac cgccagccat cctgaagaac aagtagcggc aaagaaaatc
     3481 tttcagattg ccaatcagct aaaagaagtg ttaagccctc aaagcttgct attagggcca
     3541 acgcccagca tgattttgag agtgaaaaac cgatattatt atcaactaat catcaaatat
     3601 aaacatgaac cgaatcttcc tcatgttttg gatgaaattt tgaatggaag ccagaaagaa
     3661 cagcgacaag gtctttttgt ggcaattgac aacgaaccat taaactttat ttaagaaagg
     3721 gccaaattat gcgctatcct atattgatcc accctaatga taaattaaaa agaactgctc
     3781 agccaatcga tgtaatcacg gacgagacaa tcgctttatt agacaattta tatgagacaa
     3841 tgattgcgaa tgacggaata gggatcgctg ctcctcaagt aggacaaaac aagagaattg
     3901 ctgtgatcga agtagatgaa ggcgagaaat ttgaactgat taacccagaa atcatcgaag
     3961 caaaaggtga aagccttgac gtagaaggtt gtctcagtat tccgcatgtc tatggaacgg
     4021 tcaagcgagc agatgaagtg accgtgcgtt attatgatcg agacggagaa gagatagaag
     4081 tgacggcttt tggctattta gcccgtgcct tccagcatga aatcgatcac ttagacggta
     4141 tcttgttcat tgagaaaatg atacagcaga tcccggaaga agaacttgaa gaatatatgg
     4201 aggaacatct tgatgactaa gctagtattt atgggcacac ctgctttttc tgtgccgatt
     4261 cttgaagggc tgatagaatc aggttacgag atccaggcag tggtaacaca gcccgaccgt
     4321 ccagttgggc ggaaacgagt aattacgccg acaccggtaa aagaagcggc tctgaagcac
     4381 ggtatccgtg tgctgcagcc agagaaaatc tccggttcgc cagaaatgga agaaatcatt
     4441 gaattggtgc ctgatgtgat cgtcactgcg gcatttggcc aattcttgcc tgaaaagtta
     4501 ttgcaagtac caaaattagg ggcaatcaat gtccatgctt ctttacttcc taaatatcgg
     4561 ggaggtgcgc ctgttcatta tgccatcatg aatggggaga aagaaactgg tgtaacgatc
     4621 atggaaatga tcaaaaaaat ggatgcaggc ggaatctatg cgcaagaaag cataccaatc
     4681 actaaacaag atgatgtagg gacgatgttt gaaaagctta gccttttagg aagaaagcta
     4741 ttgctagaaa cattgcccaa tattttggat ggacaaaaac cagtacctca agacgagtca
     4801 gaagcaactt tttcgccaaa catcacacgg gaacaagaag caatcgattg gcataaaact
     4861 gcagaagaaa ttgacaacca agtacgcgga atgcgtcctt ggccaattgc attcacgacc
     4921 tatgagggca tacgctggaa attattatcg gttgaagtat tggcagaaac tacagacaaa
     4981 gcacctggta cgatcatccg gaaagacaaa aaaaatctat ggattgcttg cggtaaacag
     5041 accgtcttag ccatcaatga attacagcca gctggtaaag gaaaacaggc cgtacatgat
     5101 ttccttaacg gaagcggtca gcaagttcag attggtcagc aggtgaaata atgaaaaaaa
     5161 tccctaaaaa aatgaaaaaa tcagtacgat atatggcatt actgacatta gagcgagtga
     5221 acaagggtgg cgcctactcg aatcttttgt tgaatgagat gatcaaacaa agcgagctaa
     5281 atcaaaaaga tgtgggttta tttacagaat tgatctatgg tacgattagc catcagcgac
     5341 tattggaatt ttacgttgca ccattgatcg ctaaagcaaa aaaagtggat gactgggtca
     5401 agacgcttct ttatctttct gtataccaat tagaattttt agataaagtc cctgcacatg
     5461 ctattttaaa cgaggcggtc gaaatcgcaa aagtaaaagg aaatcctggg acagggaaat
     5521 ttgtaaatgg tgtcttgagg aattatcagc gtcaaggagc accagatctg caaggcattg
     5581 ctgatcctat cgaaagactt tctgtcaaaa tcagcctgcc tctatggatg actaaacgac
     5641 tggtggatca aatcggatac gaagaaactg aaaagctcgg tttatcgcta tatcagccta
     5701 gccatgctag tgcccgagta gatacacgcc gactttccag agatcaggcc atcactgttt
     5761 tgcaagaaga agagatcaca gctgaaaaaa gccaagtcag tccatacggt atagtagcga
     5821 aaaaagggca tttagcttct agctcattgt tccacgatgg cgtcatgacg atccaagatg
     5881 aaagttcgat gcttgtagcg ccgtctatgc aaatcgagcc tgaacatcat gtcttagatg
     5941 cctgtgcagc acccggaggt aagacgacgc atattgcttc ttttttagat ccgtcatgcg
     6001 gtggagaagt ggtcgcttta gatgtacacg aacacaaagt caaactcatt gaagataatg
     6061 cagcacgtct aggtgtctct tctgctgtct acgctcaaaa gatggatgct agagaagtca
     6121 gtaaacagtt cgaagcagag cagtttgatc ggattttagt agacgcgcct tgttcaggtc
     6181 ttgggctgct tcggcgaaaa ccagatattc gttataaaaa acagccggat gaattagcga
     6241 atttaccaaa aatccaattg gaaattttag aaagctgcgc accaacttta aagtcttccg
     6301 gtattctgac ttatagcact tgtacgatct taaaagaaga aaaccaagac gttgtttctg
     6361 ctttcttgga aagacaccct gattttgaga ggattgatgt tttgaccaat cctatgctac
     6421 aatcatcaat cgaagagaaa atgttgactt tgtatccgca tcagttttac acagatggat
     6481 ttttcatttg ttgtttgcgc aaaaagtaat gaggtgaaaa aatgcagatt gaatatcaat
     6541 cagatgtagg aagacgacgt aacacaaatc aagactatgc cagtgtattt accagccgtt
     6601 caggtctcaa gcttgctgta ttagcagacg ggatgggtgg tcatcgtgcc ggagacattg
     6661 ccagtcagat ggcggtaacg aatctaggcg aagcttggga aaaacaagaa gctgttgacg
     6721 atgaaaaaat cgcccaatgg ttcattcaat cgattcaaga agaaaatatg ctgatttatc
     6781 agcgtggaca agaacagccg gaatataacg gtatgggaac aacaatcgta gcggccgctc
     6841 tgtcagatga ctggttcacg attgccaatg ttggggatag cagagcttat ttgattcgtg
     6901 aagataaaat catgcaattg acggaggatc attctttagt caatgaacta gtcaaatcag
     6961 gagaaatcag cgaagaaatg gcagtgaacc atccaaggaa aaatatttta acacgttctg
     7021 tcggcatgcc tggaacagtc gaagtagatg tttctactca tctttggcaa ctaaaagatc
     7081 gactgctgtt atgttcagat ggcttgacga acatgctgtc tgaagaagaa ataggaacga
     7141 tcatcaatca agaaggccca ttgtctcata aagtaactga gctgattgat cgtgccaatg
     7201 aagcaggcgg agcggataat atcaccgttc ttcttatcga atataaggag gacagtgtat
     7261 gatcgagttg ggcaaaaaat taaatggccg ttatcaaatc actggaaata tcggtagcgg
     7321 tggtatggca aatgtatttt tggcgcacga tttaatcttg gatcgtgatg tagcagttaa
     7381 agtcttacga tttgactttc aaaatgacca gactgcgatt cgacgatttc agagagaagc
     7441 tttagcagca acagaactcg ttcatccaaa cattgttagt gtttacgatg ttggtgaaga
     7501 agacaacatg cagtatctgg tgatggaata cgtcaaagga atggacttga aacgatatat
     7561 ccaaacacat tatcctgttc cttatgaaac agcagtaaat attatgcagc agatcctttc
     7621 tgctatttct ttagctcata accatcaaat catccaccga gatttgaaac cccaaaacgt
     7681 actgatcgat aatgaaggtg ttgtaaaaat cacggatttc gggattgcta tcgctttatc
     7741 tgaaacatcg atcacgcaga cgaatacgat gctaggatct gttcattatc tgtcccctga
     7801 gcaggccaga ggaagtatgg caacgaagca gtcagatatt tatgctttag ggatcatcct
     7861 ttatgagatg ttgacgggta gcgtaccgtt tgatggtgaa tctgctgtaa caattgcttt
     7921 aaaacatttt caagatgatc ttccatctat aaaagcactt gatccaaatg tgccacaagc
     7981 actggaaaac gtgattttac gggcaacggc taaagaacct gcagaccgtt ataagtcagc
     8041 cgaggaaatg tctgatgatc tttctacagt attatcacca gctcgtgcaa acgaagaaaa
     8101 atggcagcct catgtgatgg ataatgaaac aaaagttatc acacccctta cggaagatgc
     8161 accaatgcct gattcattca aatctatgcc tctgccaaaa gacaagccag aaaacgaaac
     8221 cttaccagag gaggaagaag ttctgcctga tcctaaaaag aaaggacaga aaaaatggtg
     8281 gctgatccta ttagctattc ttgctgtttt aggaatcgga acagctgtat tctttgcttc
     8341 tggcgggcga ggagaagttg ttgttcctga tgtttctgat ctttctgaag cttctgctag
     8401 agaaacactc gcaaaagcga aattagaagt agcaacaaaa acagaagaaa ttgcagatga
     8461 caaaatcgaa gaaggtaatg tagtgaagac agacccggca gcgggaacga cagtcaagca
     8521 aaaacgcgaa gtcactctgt atattagtag cggcacgaaa aaaatcaaat tagatgatta
     8581 cagtggtaaa agttatgaag atgccgaagc aagtttgaaa gaattagggt tttctgctga
     8641 cttgattcaa aaagaagagg aattcagttc agaggtagaa agcggaatga tcatttcgca
     8701 atctccatca gaagatacag aagtcgatcc taaaacagac acgattactt tcacagtcag
     8761 caaaggacca gaaacagtga ctttgagtag tcatgtagga gaaaactacg aaactgttcg
     8821 taattggctt gttacacaag gattcaatat caataatatt agtgctactt atgactatag
     8881 cgacactgta ccatcaggtt cgatcatcag tcaggatcca gcctcgggtg aagttgtagc
     8941 agaagaaact tacatcaatt ttgtcgtaag ccaaggaaca gagcctaaat tgaatgatat
     9001 ctccggctat acaaaaagcg aagcacaaag ttatttagct agtgtcggtg cagaatatat
     9061 cgggcatgag acttccgaat attcaaacac ggtggataaa gataaagtga ttcgtacagt
     9121 accaggggca ggaacgacaa ttaccaaagg aatggttgtg aacgtaattt actctaaagg
     9181 accagatcca gcgtcttcta gttctgaaaa agactcttca gacagttcta gcagctcaag
     9241 tagctccagt agcacaagca actcttcaaa agaagaatct tccagctcca caagttcatc
     9301 agctagcacc tcagaaagtt caaaataagt gctgaataaa atatatcaga agggaatgcg
     9361 acattgattt gttgcattct cttttttgtg tttctatctc ttttgatcaa ttttcaagcc
     9421 atatactttg agaagtttgc atggaataac acctactttc ctcttcgtat ggtaaaataa
     9481 acaaaaacgg aggtggcact tatcaaagga caaatcagaa aagcaatcag cggtttttac
     9541 tatgtatacg caaacggaga gacctatcaa acaagaggaa gaggaaattt ccggaatcgg
     9601 aaaatcacgc cgcttgtcgg agatcaggtg atctttgaga gtgaaaatca aacagacggc
     9661 tatcttctgg aaatcttgcc gagaaaaaat caattagttc gcccaccagt cacaaatgtt
     9721 gatcttggaa tcattgtcac aagtatcgta gagcctgcat tttcttataa tctacttgac
     9781 cgctttctag ttaccttgga atacgagtcg atcgaaccga ttattttttt gacaaagacc
     9841 gacttgtcag acgacccaga aaaaatcgaa gagatcaagc aaacctatac taaaataggc
     9901 tatccggtga ttgtacccaa tcatacgggt gatacgacag aactgatccg ttacttccca
     9961 gaacggctat cggtctttat gggtcagtct ggagcaggaa aatcgacgct actgaatcaa
    10021 atatcaccgg aactgaattt agcaacagga gagatttctg atgcacttgg aagaggacgg
    10081 catacaacac gtcatgtgga actgattcct atctatggcg ggttagtagc agatacacca
    10141 ggatttagct cgattgactt tttgacaatt gaagcaggag aactgccgag acaatttcct
    10201 gaatttgtag aagcttctgt ccattgcaaa tttagagagt gcatgcatgc taaagaacct
    10261 ggctgcgaag taaaacatag agtagaaaca ggtggtatcg cacagacacg ttacgacaat
    10321 tatttgcaat tcttacaaga aatcgaaaaa agaaaaccaa tgtatgcaaa aaaagaaggt
    10381 aaacgaaaat aggaggaaac aagaatgaag atagcaccat ccattttgag tgcagatttt
    10441 gccaatttac aaagagacat tgagttagtg gaaaaaggag gcgcagatta catccacgta
    10501 gatgtaatgg atggccaatt tgtcccaaat atcacattag ggccaaatat tgttcaggca
    10561 atccgtcctg ttactaaatt accgttagac gtccatctga tgatcgttga tccagaaaac
    10621 tacattccag catttgcaaa agctggagca gatatcatta ctgtccacgt cgaagcaact
    10681 cctcatattc atcgggcact tcaaatgaca aaaagcttag gagttaaagc tggagtagtt
    10741 atcaatccag gaacaccagt ctccatgatc aaacatgttt tgcctttggc tgatcaagta
    10801 ctagtcatga ctgtcaatcc tgggttcggc ggacaaagct ttattgaaga aacagtagaa
    10861 aaaattacgg aactcgctga tctacgtaga gagaacaatt ggacttatgc aatcgaagta
    10921 gatggcggaa tcgttccaga aacagcaaaa atctgtaaag atgcaggggc ggatgtcttc
    10981 gttgcaggat catatatcta tggagccgaa aatccagtcg gacaaatcga acgattaaaa
    11041 gaggcattag actaaatgaa aattttatta gctgctggag gtaatccgca aaactggccg
    11101 cagtctctgc tgtccaaata cgatgcctat gtcgggatag accgtggtgc gctgtacctg
    11161 cttcaagcag gctacccagt agatctggca gtgggtgatt ttgattcatt gtcgaacgaa
    11221 gagcagaagg aagtatttgc aaaagccaaa gagtataccc gttcccctgc agaaaaagac
    11281 gatacagata cacagttagg tatcttaaaa acatttgaac gttacccgca agcggaagtc
    11341 actttgattg gagctacggg cggaagattg gatcatcttt tagccaatct ctggttaggt
    11401 atggaacctc gcttcaaacc ctttgcccat cagcttatta tcagagattg tcaaaattat
    11461 ttgtcttatt atttgcctgg agagcatata gtaagacaga taccagatat gaagtattta
    11521 gcttattgct gcttgactcc cgtctcgaat cttacattaa aaggcagcaa gtatgtattg
    11581 gaaaataaag aagtagaata tccgacatct tatgcaagta acgaatttct ttcagatacc
    11641 gcagaatttt ctttttcaga tggtatgatt gcagttattc aaagcaaaga ttgatccata
    11701 aacaaataaa aaaatttgtc tttcaggcta aaaatgacag aatctattat ttgctaaaaa
    11761 aaagcttaag aggagcaact taccttttgt ttcacggcat ttagtgataa aattgaacac
    11821 aagagtctaa ttaattcgag gtgtctttgt gaagaagttc aatcctaata aaaa
//
LOCUS       DACENJ010000075        11806 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.075, whole genome shotgun sequence.
ACCESSION   DACENJ010000075 DACENJ010000000
VERSION     DACENJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.075"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(90..668)
                     /locus_tag="IW748_001451"
     CDS             complement(90..668)
                     /locus_tag="IW748_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1104368.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(688..1356)
                     /locus_tag="IW748_001452"
     CDS             complement(688..1356)
                     /locus_tag="IW748_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104369.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1371..2441)
                     /locus_tag="IW748_001453"
     CDS             complement(1371..2441)
                     /locus_tag="IW748_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1104370.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2612..3976)
                     /locus_tag="IW748_001454"
     CDS             complement(2612..3976)
                     /locus_tag="IW748_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1104371.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(3973..5532)
                     /locus_tag="IW748_001455"
     CDS             complement(3973..5532)
                     /locus_tag="IW748_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1104372.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5588..8050)
                     /locus_tag="IW748_001456"
     CDS             complement(5588..8050)
                     /locus_tag="IW748_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104373.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGIPLQFDVTANVAKMTENGDKYDTSPN
                     KILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGGNLNPGA
                     YAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLTMGLTEG
                     RFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQGGLLSP
                     VALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIHYRLYSK
                     GTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTNMYPSFA
                     YKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQENVTFT
                     LTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLSKIGDTI
                     HLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTILVPKQEL
                     TLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLTSLFKND
                     QGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLLEVNSST
                     NAQPGATYQGMVTWELTAGPTS"
     gene            complement(8034..8369)
                     /locus_tag="IW748_001457"
     CDS             complement(8034..8369)
                     /locus_tag="IW748_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104374.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8383..9417)
                     /locus_tag="IW748_001458"
     CDS             complement(8383..9417)
                     /locus_tag="IW748_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1104375.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKVETT"
     gene            complement(9507..10187)
                     /locus_tag="IW748_001459"
     CDS             complement(9507..10187)
                     /locus_tag="IW748_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1104376.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10471..11574)
                     /locus_tag="IW748_001460"
     CDS             complement(10471..11574)
                     /locus_tag="IW748_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1104377.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         3395 a         2443 c         2037 g         3931 t
ORIGIN      
        1 aaaactacct atataaaaaa aacaagatat aacgatcttc gtcatatctt gcttcttcac
       61 ctaactattt ttctccctat ataacagctt tacgcttctc ctaatacacc attcaaatat
      121 aaatcgatca attgcgccac atttagcggt tcttccacag gtcttttcga ccgagtgatg
      181 taaggtgcta acactaagaa aaaatgcctt gctgcgattt cttttgaaat cccatttctt
      241 agcacagatt ggtttgctga aaagatttct tcaaaaggtt cacgataatt tttcttccat
      301 agaaagaaaa gatgatctct tgtttccttt gaaagattgc tttcaagatc gtgaagcatc
      361 agaaacaaat cgaccagatg tgtctcaatc aaaaactgcg ccatttttgt caaggttact
      421 tgaaatcccg tttcgcttgt ttgaacgatt tctctcaaag cttttccaac atcttttaag
      481 tggttttcta aaactgccgt atatagtttt tctttatcac cgaaataatg atagagattg
      541 ggttgggtga tcccaacttt ttgtgcgatc tctcttgttg aagtgccgag atatcccttg
      601 gacataaaca agtcagtcgc ttctttcaaa attttttgct gtgtatcttt gttgtatcga
      661 tggaccattt ttccacctgc tttttggtta ttctttttct ttcaatacac catcttccat
      721 cataaatatt ctatcgcatt tgtcaatcag ccgtgtatcg tgagtaacca tgatcgttgc
      781 tttatttttg tcttttgatt cttttgccaa gatatcgact acttctcgtg ccttttctga
      841 atccaaacta gcagttggtt catctgctag aataatggac ggatcattgt acaatgcacg
      901 agcgattgcg accctttgcc gttctccacc agatagttct tctggatact tgttgatcaa
      961 cttatccacg ccaagctgct caaataattc ttttgcttct ttttgacgat gttcatgttt
     1021 gatcttatct actaataata actgcttttt tactgtcaga aaaggaacca gattggaagc
     1081 ttgcaaaata aagccaattt ctttaaaacg tagtttaacg cgttcacttt ctttttgatt
     1141 actgaattct tgatcattga taaatacaga gccactgcta ggcgtctgca atccgcctgc
     1201 caaagtcaaa aaagtacttt tacctgaacc agaaggaccg atgattgcaa caaattcccc
     1261 tttatcgata ctcatggttg tttctttcaa tgctttaact acttggtcgc catcttggaa
     1321 acttttttct acttctttaa attctattgc tttcatgctc ttcctccttc ttatccaatt
     1381 gccttcaacg gatcgatttt tacgattgtc cgaacagaaa atagtgcgcc taacacagct
     1441 actaccagca tcaacaagct aatgacgcca aaaaacagcc aattgctttg gaatggaacc
     1501 gcatcaggta gaacaaggga tgttcccact gttcctaata gaccaagaag aataccaaca
     1561 aatgataaaa tgaatgtttg tgccactaca gaacgcgcaa taaatcctcc tgtgatccct
     1621 tgtgctttca ttacaccaaa gatattaatc ttttgcatcg tcagtacata aatgaaaatc
     1681 ccgatcacaa tagccgcaat aacgatcaaa aatccgatca taaatccaaa agtcaatact
     1741 tgcgcattat atccaggtaa ttcattgatg aatttagaaa ttttgatctg ttctaaatct
     1801 tcaggtaaat tactgatttt acctctgaca actattgcgt taatccgtgc attttcactt
     1861 gtatcttctt tttcgaagcg gatctcttgg taagcattga tggttgtata gagaactggt
     1921 gagacattga atttggcatg atccgtaaag ccagttagtt taaacgtctt gtcactacca
     1981 gataacttaa ctgtatctcc tacggctaga ccatattctt ctttcaaact aatatcgcct
     2041 actgcctcat catcgttatc aaaagctttt ccttcaacaa gattgggcat gatgaattga
     2101 tttttatcga taccgaagaa gctaacattg atttttcctt cttctgtact gtctttacta
     2161 gtcacgactc caggtgtctg ggcaagatac gccactttcc caccttcgac ttcttctgct
     2221 atcttcttag taatcatgga catccctaga tttgaatttg catctttcga aagcacaatc
     2281 gaatctgcct cccacttgtc gactgctgtt cgattatctt gagctaagcc ataagctaac
     2341 ccagtcaaaa aaaataccag gtaggcgatc aaaaacatca cccctgcaac taacgcataa
     2401 cgtaactttg aatgcataat ttcatttaat gctaaaaaca tttaactcct cctcctgaaa
     2461 taatcatttt atacaacaat tttatcactt gataaatttt tggcgaatat tatgctcaaa
     2521 aaattcttcc gctatttcat tatatgaatt ttttattctc gattgattaa tatttattat
     2581 atccccacac aataaaagac cgtgatattt ctcacagtct tttatctact ctattgctat
     2641 ttgctctctt agctagtact ttaaaagcta actcattgag gtagtgaaaa cgagaggtgt
     2701 gttcagacag aaatccaaaa tcctcaactg ctagctgtgg aatcgtagtt ttgtgtttca
     2761 cgatatccgc aagcaggttc aaatctgata ataagaattc ttggcttatc agctgtccgc
     2821 caattatttc taatgtatcc ggatggtaga tcagctttgc atttactgtt ttgttttctt
     2881 catcgtattg tggaaataga tcaaattctc ctgaacacga accggtattt tgttccaaga
     2941 atggcgcttc atcttctgtc agccctacag ttcctaaaaa gtagtctata atgactgctg
     3001 ctgttatctt ttgtctgagg tcgtaagcgg ttggctgacc actcaaagtt agcgctactg
     3061 ctcttgccat atgtacagct cgagtaacta aaggtaaaaa gagatgccgt ttatttttgt
     3121 caaaactgac tgggacgata tctccaatcg catagatgtc cggatcactt gtctgcagat
     3181 attcattgac tatcacagtc tcatcctcat acaaagccac tttttctttt aacaatgtcg
     3241 tattaggacg agtttgggca gaaaaaatga ccatatctgc ctttattgct tgagatagaa
     3301 gttgtacgga tactactttc tcttctgatt ttttccaatc gatcaagaat tcatttggat
     3361 aaaaattgat ttgcttagtt tgcagctgat tttgaaaact gttgatcata tccttatcga
     3421 aatatcttga taaaacattc ggcatataat caactagatg aataatgtat cctttatttc
     3481 tcaaagcatc tgctaattcc aatccaatat aaccagcacc gaagatcact acttcttttg
     3541 cttcttttaa agcggcaagt acttgcttag attgcactag atttttatag gtgtagacat
     3601 tttcagcttc aatttgttta tacaaggtga caggagagga acctgttgct acgattaatt
     3661 tatcataggt tatggtctcg ttttttcctt ctttggatgc gaggatgagc tgatgtttgt
     3721 ctggatcaag ttcagttact ctccattcgg tgatcagttt tgccccactt tcttgaagag
     3781 tccgcatgtt atgggcaact tcagctaaac tacgtatctt tccttttaaa taaaggttga
     3841 ttccatttga aacatagcct agtccatctt tatgatcttc atcgatcagt aaaacttctg
     3901 ctttcggttg aagttttctt agatttaggg cagctgtgat gcctgcatgg gaagcaccaa
     3961 tgatcacgat tttcatgttt tattcatcct taaattcaat ttttctaaat aacattcttt
     4021 taaaaattgg ttcaaatagg ggaaatcata attatattcg ttgctagtga atacaaaaac
     4081 tttagcggac ttctgtgacg ggtactcttt caattggcta gtcaaaatga tatcatactg
     4141 cttttctttc tcgtattcga ctaaatcata tttgatcgta ttcagcaatt gttcctggat
     4201 gatttctttc acgatatccg ccattagcct ttcataagga agatcataag caatttgcag
     4261 acgtaattct aactgcttga tcagcgtccg caaaatagat gcatagcgag caaataattc
     4321 aaagcgggca ttttcctcgt ctacttcagt aaaatcaaca atagcaaatg tttgtctgac
     4381 caatctttca cataaaacca ggaattgttt atcttctact tcagttaacc gctgttttaa
     4441 tccttcttgg ccaaaaatat tcatccatcc tctaaaatag gcaaaatgag cgtgattttg
     4501 catcactgtc aacgcaatct tttgcgaaat ctcttctgga aaaacataaa aagagaaata
     4561 ttcttctatc gattgtttaa acgttcgatt caattgagct actacgtcgt ctttgtattt
     4621 ttcgcttatc aaaaactcct ggacatgcga gttatgtgta tctaatgtaa agttactgat
     4681 catgaaaaga taaaaaacca acgtttcatt ttgattccaa ggaaaggcat attgaaagaa
     4741 gtaacgggtc aaaaatgtat ggaggtccaa taaaaattgt gtcgtttgaa acaatccaac
     4801 aggaagattg ctataatctg gtttggattt tttcgtacag cgcattcgct tcttcataat
     4861 ggaaagccat atttcaaatt tcaatcgtcc ttcttctgta atagtaaaac taaactcttt
     4921 ttgcagcaga tctaaaaaag tgtttcctga taaatcactg tattcttttt tgttttcaag
     4981 ataaggacgt ccaaaccaaa ataattggaa ataaaaataa caaatttgac tttctggacc
     5041 ggtcagccgc ccgcttttta ttttcagatg aaactcattt aaagaatcat ttaattcctt
     5101 cactctgcga gacaaagtag ccgaactgat attaagttcc atgacaagtt tctgccgatc
     5161 aaatgagcca ttttccaaca gataaagaag tatctgatat tttgtagaat agcgcaagta
     5221 gcaatgataa taaacatcga ctgctgctat atttccatgt ctgattaata aaatattccc
     5281 ttcttggata acaatttcca attgtttgat attttccaag ttagcttgta aataaaacag
     5341 atcttctttc aaggtagccc gatcaactga taagcgggta agtacgttat taacggaaat
     5401 ctctcctttt tcctctaata aaagttggaa aagtttgatt tgccgttgtt catttttttc
     5461 aagcaaactg aaaagttcca agtccactcc tccaagcatt tctgatttta acagcagaag
     5521 tttccttctc atccttgaaa aagaggatct gtgacagtta tcacagatcc atacaaattc
     5581 taatcactta gctggttggc ccagctgtca gttcccatgt gaccatgcct tgatacgttg
     5641 cgccaggttg agcattcgta ctgctattta cctctaagag taaaccatcg gtatcgggaa
     5701 agtcaacgag aacctctcct ttagctgttc catcactctc ataaatcaac gtgttttgac
     5761 cttgctgaat caattgtttt gatccagaat gattgaagta taattttgtt ccgcttaatt
     5821 cttgaccttg atcattctta aataaagaag tcaatgtccc tgtcagttgc caaggagacg
     5881 tgcttggaag ccgagtatcc cgcacatcaa aagaaaaatc gccttttgct tcataataac
     5941 ttgtcttcaa cggtttgggg agatcgttac caaatgtaaa atcatctggt acattaacta
     6001 atgttaattc ttgtttgggg acaagaattg ttttagcaga tgtttggaca actgggttag
     6061 gaagcttagc accatctgct aatgtaatat tgctggaaag tacagctgcc tttgtcagta
     6121 tgctgacgga ttgtacagaa gtatctacag tatttaattg atagttgtaa gtaagatgaa
     6181 tggtatctcc tattttgctc aatagatctg ctgggacaga aactccttgt tgaatgccgg
     6241 tatctgcgat agttgtacca tttaatttta aggaaccatt gatataagta gtattttcag
     6301 gaagtgagat cgtgattttt ttgtcggtta tcgcatcccc agattttgtt aacgtgaatg
     6361 tcacattttc ttgaatcgta tctcctgata gagcagacag gggatctcct gtctcaggtt
     6421 gtctgctaag gttttttagc tgactatctg cactcacaga tggaaaatta gccactgtta
     6481 tttgaggaag agtggtgatt ccagcttgtt cagactgcca tttgtacgca aaagacggat
     6541 acatgttagt aagtacatcg tccaccaagc gataatcaaa ataatattgc cctgaagcca
     6601 atgcaggtag tgtagaagta gctgtattta cacccccgtt atttgattgg atcgtttgga
     6661 aaagttgatc tgtccatcct gtcgctaatt tttgggttcc tttcgaataa agcctgtaat
     6721 ggatcgaacc cttttttagt tcagttgctg accaattggc cgtgacttgg tacgatgcag
     6781 aagaaaggag ctttccccac tgactatcgg ctgttaagtt tgttggtaaa accgttccgt
     6841 ctagcttgta ctgctccaat acctttaaag caacaggaga aagcaatccg ccttgcgcat
     6901 ctttcccact gtacacgata ttggctgtct gattcaatcc acttgtgctt gctggagtat
     6961 gagtcagtat tgctccttgt gtaacgtttt ttcctgtaaa atagttgact acgcgatcaa
     7021 agcgactaaa tggcaactga tcaaaacgtc cttcagtcag ccccatcgtc aatgtccgac
     7081 tattggtaga gcctggaagg ttctggttat ttccatccac gtattctgtg gtatcagcaa
     7141 atgtattata ttttcctgtt gaatcacttg ttgagatgta actattccac gtatttatct
     7201 ctgcacttgc agaaaatgca tatgctcctg gattcaagtt tccgccagtt aatttaggat
     7261 aaaatctacc tgcagctgga gctaaaatcg aattgaacat acgatcttgg ataatattat
     7321 tcgaatcata aaaatcaaca gaaaaaacgg cctttttaga catagaaggt tgtaatttac
     7381 ccttggtcaa aattttgttt ggactcgtat catatttatc tccgttttct gtcatcttcg
     7441 cgacattagc tgtaacatca aattgaagcg ggatacctcg cgtaataata gcaataatat
     7501 ctgccaatag agtatttgta tcccacgacc ccatcccaaa acgcaaggta tggcttcctg
     7561 ctgataataa actaccgcta gtaaccttga tgggggcacc ggcaatatac aagtaggcgt
     7621 tgttccagtc aattgctttt aatacatcac tgcttgatac atgttccggc aaattgatga
     7681 tcgccacaaa tgcaaatcct tgtgcagtga tattcaccaa atcaagtaca tttgttagtt
     7741 tcacatggct ttcccaatga aaacggaccg tgccaccttt ccaaggatca tttgaaccat
     7801 cgttcaaata agtagcagtc aatttaggca attggttagc cccagttagt tggacaagct
     7861 tcccgccatt gactgcacta cgtccaccat ttggaggtac gatattttca tacgtgtatg
     7921 gatcataaga aatggcagga ggcgttgctc caacagcttc tgccgaaggt gaaaagataa
     7981 atatacttaa aacaaaaagc gaagtaaaaa agaaggagcg aaaaaatgaa aatttatttt
     8041 gtagcaacat gttcatcctc cttttccttt cttctccata acaacaacca cgcaagaagc
     8101 cataattcta taccaatgat caaataaaga aaacggatct tttcttcatt cgtctccggg
     8161 aatacgccat gttgattaga ttctttataa atcggtagct gtgtctgctg ttttgatgcg
     8221 ccattgactg aaccacttgg atgcggaact ggacttagat cataaaatgt aatcgtacct
     8281 gaacttttgg tatctgcttg gactggctct tgccagctga agaacagcaa acttgcaata
     8341 atccctccca ataagatcga tgttttcaac gaaatcacct catcatgttg tttctacttt
     8401 tttctttttc ttgtggaccc acaaaaacag caaaatcaac cataacacta gaactgcagc
     8461 aaccaatcct gcataaagcc atgggaaaga ttgttcttgt actgcaaaaa cggaattttc
     8521 atttactttt tgtgcttttt cttttttgat cgtaaagttt tgtgtaaatc gccattcatt
     8581 actgtcgcct gattttgcta tgatttctgc gcggtaatcg ccggcttttg ctttatcacc
     8641 agctaaatca agattcaagt gaaaattcga atttggtgca actaaaaatt cattagacgt
     8701 attttcaata taaagctgat tttcataaaa gatcttgctc gtaaatatca aattgctgat
     8761 aatcctaggc tgaggatttt ggaaacgaag ctgaacagtt ggctttgcgt tgacctcgtc
     8821 caaatctcct gataaaagtt tcagatccgg ttctatcgtc tcttgacttt cttgtaaaac
     8881 gactgcgaga ttataagcaa tacgattttt gatttgtgct ccttctgtag cattttctgt
     8941 ttcagcttct tctttaggtg aaacatgaat acccgctaat acacgtccct taaagctttt
     9001 tgctgggatt ttcacttcta taggtatagt tgtttctgca tgtgctgata cattgatttc
     9061 ctttttcgca atgagcaaaa cgtcacggac atcaaacggc aatgtctcat ctaacgttgg
     9121 gtttttttga ctataatcga tcgttccacc atcactcgtt accgccggat tgacagagac
     9181 ctcaaatgtt cgttcttcat ctgaagaatt gctgataatt acttctagtg tctgattttg
     9241 ttccggtgcc actaataaat caaaataacc gattgcttta tctatttggt tctctggaaa
     9301 taccggtttg attccaaagt ctcctgctga atcctttgct tggctgattg acggaaaaat
     9361 gaaaaataga cttaaaatag aaaaaaacga aacaaacagt cgtttacttt gattcattgt
     9421 ccaccacatc ctttcaacaa caactgcggt aaaaaatcct tcacctatag tattttcacc
     9481 gcagtttgtt gaatttattt catactctat gctactgggc cagccacgag gttccaagtc
     9541 aaatcagccg tgaatgttcc agctgctgct ttcggcactt tcagtgtgat atttgctgga
     9601 tcataaacag aaagccatgt tcccagacct tgatcttttg ctgctgtaaa tattggtgtc
     9661 gctgcacttg ctttactcaa cccagttaca gtttgactag ttggtccagt ggattcgtta
     9721 tctgacgtac ttttaacagt ggatgttcca aaatctaatt cagcgccttg caaaacttgt
     9781 gaaccgttta caaaatctga aaccgaaact gtcacgttcc acccttgtgc ttggccatct
     9841 gcacctacac ctcgacgatc tgatacttgg agataaggtg tatcgttttt tgaagcgtct
     9901 acttgatacg tttttgttcc gctttcaatc tcgtgtgtac caaatggcaa ttcaggaact
     9961 acatccaatg ttaaagaacc tgtctggcca gttgctggat tttccggatc tgctggctct
    10021 actggttttg aaggatcagt agggttaaga ggctcaacgg tatttgttgg ttcagtaaag
    10081 gtaatatctc cttttgaagt cgcttgaccc tcagcaaaag ctggtgttgc caagccgcct
    10141 agaagaagtg ttgaaagtaa taatgtccca atcatgttct ttttcataat aaatcactcc
    10201 tttttcttag tgacctaagt atagtagaga cagtatgcaa aaaatcctca tgaattcatc
    10261 cgcttacttg aaattatgac cctttttgta taaaagagaa aaaaacaata aaatcgttta
    10321 catgaatatt cttccttttt tattaagtat gaaacaatat ttcaatttta ttttttcgtt
    10381 tcaaattatt ctattagtga catccttgaa ctataaaaat aggagtacgg catcatctgc
    10441 cgcactccta tttttttcgt catttatttc ttaaataaaa cgattctcac gcaattttat
    10501 ttttttgata tcttctactg tttgtgttcc agctaattgc atgaccattt ccaattctgt
    10561 tttaaagaaa tcaaacactt gatggactcc tgtacttcct cctaaagaca atccataaat
    10621 aacgggtctg ccaatagcaa caagatcggc acctgatgcg attgctttga aaacatgttg
    10681 tccacgtcgt acaccgctgt caaacacgat tggtacacgt ttatctactg cttctgccac
    10741 atattgcaac gaatcaaacg cagcaggccc cccatctaat tgacggccac catggttaga
    10801 tacccaaatt ccaccagcac cagattctaa tgatcgatag acatcttctt ctgattgaac
    10861 acctttcacg tagactggca aatcagaatg agcagcgata aattctacat cttttggact
    10921 aagtttttgt ttggaagatt tatatacagc atccatcgtt tggcctactc ctgattgata
    10981 agcctgaacg atcggcattg gcagcgggaa ggtaaacccg tttctgcggt ctgtttcacg
    11041 gtttccgcca actgttgcat ccgctgtcaa tacgattgct tttgctccat tacgcttggc
    11101 cacttcaaga atatccaaat tgattccatt atctttgctc atataaaatt gaaaccattg
    11161 cggtgctttc tccccgcctg cttctcttat ctcttctaaa gtacaagaag cgtaagaact
    11221 tgctgtatag atcgtcccaa aatctgctac accttttgca gaagctttct ccgccttcac
    11281 atgagctaat ccatgagcag caactggtgc catgatgatt ggtgcagtca gcatttcttc
    11341 gtcaaaatgt gtagttgtat cgggtaattc tacatctctt aatacatgag gaataatcaa
    11401 acgatgattg aatgcccgtt cattttcttg ataagtaaat aagtcgccag caccactgct
    11461 gatgtaacca taaccaccag ctggaatcac ttgtgaagcc gcgatttcta aatcttccat
    11521 attgataaaa tctactattc cttcatttgt acctgcttga tatgtttttt ccatctcaat
    11581 gccttctctc tataaaattt gaatgatgaa caatatgata gcgtttacac tttttatatt
    11641 actcttcgac attgatacag gcaaataatc tgtttacatt tcagccattc cagatgtcac
    11701 tactctggaa gaaatgattg taataaaaat agataaaaaa ccgatgggac tttgacaaaa
    11761 gtagtgtcaa agtccctttt tttgaataaa cggtgttcaa aagcca
//
LOCUS       DACENJ010000076        11592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.076, whole genome shotgun sequence.
ACCESSION   DACENJ010000076 DACENJ010000000
VERSION     DACENJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.076"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            173..472
                     /locus_tag="IW748_001461"
     CDS             173..472
                     /locus_tag="IW748_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104378.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(569..1003)
                     /locus_tag="IW748_001462"
     CDS             complement(569..1003)
                     /locus_tag="IW748_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1104379.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1235..1945)
                     /locus_tag="IW748_001463"
     CDS             complement(1235..1945)
                     /locus_tag="IW748_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1104380.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1935..2795)
                     /locus_tag="IW748_001464"
     CDS             complement(1935..2795)
                     /locus_tag="IW748_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1104381.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2800..3438)
                     /locus_tag="IW748_001465"
     CDS             complement(2800..3438)
                     /locus_tag="IW748_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1104382.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3453..3752)
                     /locus_tag="IW748_001466"
     CDS             complement(3453..3752)
                     /locus_tag="IW748_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1104383.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3795..5279)
                     /locus_tag="IW748_001467"
     CDS             complement(3795..5279)
                     /locus_tag="IW748_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1104384.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKATCLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5299..5760)
                     /locus_tag="IW748_001468"
     CDS             complement(5299..5760)
                     /locus_tag="IW748_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1104385.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5778..6845)
                     /gene="ulaG"
                     /locus_tag="IW748_001469"
     CDS             complement(5778..6845)
                     /gene="ulaG"
                     /locus_tag="IW748_001469"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1104386.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7063..7833
                     /locus_tag="IW748_001470"
     CDS             7063..7833
                     /locus_tag="IW748_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1104387.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8106..8933)
                     /locus_tag="IW748_001471"
     CDS             complement(8106..8933)
                     /locus_tag="IW748_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1104388.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9501..10736
                     /locus_tag="IW748_001472"
     CDS             9501..10736
                     /locus_tag="IW748_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1104389.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         3597 a         2391 c         1825 g         3779 t
ORIGIN      
        1 aaaaaaataa atgtttgtat accatatgat tatttcaata tgtatcctgg aattggagat
       61 ctcgtagagc tatatcaaga tgaatcaaat atacttgtca ttcatcatga aatatagaga
      121 cttataaaat tgtttatttt tataatgtat cagtatagaa aggaaataaa acatggatta
      181 tactgttgaa tttgacagtg aactacttaa gcaatttcca caagaagaac atttcatgta
      241 taaagaagca atttcccaca aagtgacaga gtatttggag cataattttt ttcgtataaa
      301 gccagtgcgt actcgttcgg caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa
      361 aaattatcgc atagcatttg ctttaaaaaa aaatcatgtg ttcatttttt atattagttc
      421 gaatttgcaa aaaattgtat ttgataaaga ggtgagtaaa aaaataaaat agctgagcat
      481 tggaggatag attgagtgat actaagtcta tcctcttgtt tttctaaaga actaagctag
      541 aattttttct tttgtacgtt attatatgtt atttgtcatt accaaaaagc accttttttc
      601 ttctagtatc tatgacataa gaaataccac ccatttcctt tacttgaaca aatcctactg
      661 attcataaaa tttttttgtt ttctcagtat catctgtcaa taattcgatt tggactggtc
      721 cataactagt gatcttctcc aaagcttcat tcaaaagcac tttgcctatt ccttggcgct
      781 gatacctcgg atgaatgagg atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc
      841 aacgaacgat tccgataatc ttcccttccc cattacgctt tatcagagat tcagattgtt
      901 caaatgctct agctaacctt gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat
      961 ataattcaga aatctcatcg atcataaggg aatcaatatt catttttttc ttcctttctt
     1021 atacaattct ccctctaata atacagttct ttgactattt aatctatcac atataactct
     1081 cctgtaaaga ttctctcgat tccttaaaat tcctctttcc ttttagaatg catacttttt
     1141 gataaataaa aaggttgtga taaattttct tcacaacctc tgtttctctc tatcaagtgt
     1201 tatcttttaa ttttctaccc ttaccctagc atctctattg cccgtagtat gcatactttc
     1261 catgtttgcg aaaataatgt ttatctagta aatattgcgg gatttcacat atattggggt
     1321 tgatcagctc gttttctttg gccatcagac aaatttcatc taagatcaaa ctgttttcta
     1381 ctgctttcat aggtgaatcc ccccatgtga acggtccatg tccatagaca agtactccag
     1441 ggacttcgtt tggatctaat tttctttcct taaacgtctc gacaatcaca ttccctgtat
     1501 gaacttcgta agcttctctt acttcttctt tcgttaattg gcgtgtacaa ggaaccttac
     1561 cataaaaagc atcagcatga gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc
     1621 acatgacgga gtgcgtggaa tgagtatgag taatagcagt tatatcttca aaagtttgat
     1681 acaaaacaac atgtgtgggg agatctgaag atggttttaa gctatcttct tctattatat
     1741 ttcccgacaa atctgttacc accatttgat ctgcttgcat gcattcatat ctgacaccgc
     1801 ttggcttaat gacgatgact cctagctcac ggtccacttc actcacattg ccccaagtca
     1861 atttgactaa tccgtatcgc ggcaatgcca gatttgcttc aaggacacgt tgcttcattg
     1921 ctgcaatttt ttcttcacaa gacatcatag ccagcctcct tcaatttaga acttaagtag
     1981 tcttttgcct gctggatttc tcttcgaaag tcttggcttt tttcactcca catttcaatc
     2041 agaaatgttc catcataatc tagacgtttc aaatgtctta ataatccaag aaaatcaaca
     2101 caaccgctac caaaaggaac atcacgaaac tggcctgaat attcttttgt tacagggtaa
     2161 gtatctttta agtggatagc agtgatataa tcgatccctt tctcaagttc tcgagccgga
     2221 ttattttccg gccatgcgga caaattgcct aggtctggat atacttgtag aaaaggtgaa
     2281 tgaatctgtt ctttgatttc taaaaatttt gaaatggagt tcataaatgg atcatccatg
     2341 atttccacgg aaagaatgat gccatattta gaagcttcct ttacgctttc ttttattcct
     2401 tcgataaagt cttctctgga attcattgat ttctcttcat aataaacatc gtatccagct
     2461 atttggatta ctttgacgct tagattatgc gccaaatgga ttgctttttt cattagttgt
     2521 ttcgctgctt ttttcttttc tggatcagca gaaccaaacg gaaatcgccg atgcgcactt
     2581 aaacaaatcg aatggatacg tacaccggag ctgaacatct cttctctcag ctcagctatc
     2641 ttttcttcag accaatctaa tcgtgctaac cgctcatccg tctcatcaat cgacatctcg
     2701 ataaaatcga agtccatttc ttttacaagg gaaaaacgtt ctttccatga aatatctttg
     2761 ggtaacgctt tttcataaat cccaatttga gccatttcct tacccccaga tacgaatgat
     2821 ctcatctttg aagttttgtg cggccttcat cggatcattt gctgctgtaa taccacgacc
     2881 agtgataaag gtatacacat cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc
     2941 accagttaca gaaacattaa agcctagttc aatcagtttt ttgatcttcg ataaatcttt
     3001 ttcaccccaa gtttcaccag ataacagcgc atcacggctt tgatggtaga ttacctgccg
     3061 gataccgatg ttatgccaat ccatggcttg ttcaaaagtc caattgccgt agagttctac
     3121 ttgcaattca cctatttctt tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat
     3181 gactgtcatc cagtctgctc ctgcttttgc aacattactc gccactgttc ctccagcgtc
     3241 tgcacatttc gtatcagcga ctagtttttt atctgggaac atactgcgaa tacagcgaat
     3301 cgctttttgt ccttcctgta agcaaagaat tgtgcccact tcaataatat ctactacatc
     3361 accaactttc ataacatctc ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg
     3421 caaattcggc ctgctcatct tagttccttc ctttacttgt ttttttggta ccactttgtt
     3481 tcagcaaatt tttcactgat ttctttgact gacatcacat ttttgatacc gataactgta
     3541 actccttttt tctctgcctg agtaaacata ttcaaaaaat tgactggcgt aaaaactaca
     3601 tcgtattggc cagctgaact ttttccctct gaaatggcac aatgatggat tttggtaatt
     3661 ggtacgttta gttctttcat tgctttttcc acacttctca tcatcatcaa gcttgttcct
     3721 gatccatttg cacacgatac taaaattctc atcttaatcc ctccattatg agtgttgtct
     3781 aatcagattt tcttttatac tctctgcttt tctgcatatg tgtcatagtc ttctacaatc
     3841 aaaaaataac catcaggatt tttccgatat tgtaactgcg gaatagccaa taaaccaatg
     3901 acaactacgg caattccagc ataacctagg actttcatga ttactgtaaa caatggccaa
     3961 gctgttgccc agtcgaacat cccgatataa ccaccgtatg ttgacatccc aatccatgta
     4021 gcgaataatg ctgaaccgcc tacttgtatg attcctgaaa gaaacgggaa caggcaagtg
     4081 gctttgaatc cacctcggtt attggcataa actgctataa cggcattatc aaagaacaac
     4141 ggaatgaaac cggctatgac gatcgttggt gatttcaata aaatcagtaa accaatcatc
     4201 aaaaactgtc ccaaagcacc aaacaagaac ccaatcgtta ccgcatttgg cgaaccaaag
     4261 ccaaaagtag cagctacgtc aattcctgga acagctcctg gtaaaatcgt gtttgagatt
     4321 ccttggaaag attgagttag ttcatcgacg aatgtccgaa cacctaattg caagatcgcc
     4381 agatataccg caaaatttaa agaagtcgtc ataatgtaga ataagaaact ctgcccttct
     4441 tgcatgaatt gcgcttgaat caaataatct ttgcctagta caatcagaat gatcccaaag
     4501 aaaaatagca tgagtaacga agtagcaacc atattctcat tgaaaatcga caagaatcct
     4561 ggtaatttga tatcttcaat ttttttgtct tgcttgattg atctattttt cttttcatcc
     4621 cgctttttca tccactcagc taattttgca aatataaaga ttccgaacat ttgctgatgt
     4681 gccactgcaa atccggcacc ttctgtcaat tcctgcgtga ttcctactgt taagttagaa
     4741 gctactgtcc aataacaacc taaaatcagt cccataaaaa tcaatgcttg tatcctacct
     4801 agatcaggaa agcaaaacag taaaatccaa aaagcggtag ctgcttgttg aacctgaacg
     4861 tttccggtag tgaaaacgga acgcagtttc gtatatttct gaaaacggac taataagata
     4921 tttaaaacaa atgcaatcag taataaaatc atcacatctc caaaggtacg gccaaatgtc
     4981 tccattaatc cagcatccac agcattttgt ccaaaataag gatctgtaac catggcatcc
     5041 aaattgaacc gttcctttag tccaaccaag attggccgaa aattattgac caaaccacca
     5101 gagccaacag tcaaaataaa gtaaccgacc gttgccttta agaatccagc gatgcattca
     5161 tgaagcgggc gtttcagcaa aatatagcct aataacacga taaaaccaat caggtatgct
     5221 ggctgcgtca agatatttgt tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc
     5281 ttttttctcc tctatcaatt aattatattc tgccatcaca acttggtaat catcgatgga
     5341 gttcgtagag atgagacgct caaccaatcc atctgtcatt aataattcgg ataatttttg
     5401 gatattttct agatgttctt ctggatcttt tgctgcgagt gtaaaaaaca aactagccgt
     5461 tttctcctca ttctcccctt cactttcaaa acggattggc tgtttcaact tggtaaatga
     5521 aatagctgtt ccaaaaacac catcactttt ttctgaagaa tgcggcattg ctacttgagg
     5581 tgcgatcaca atataaggac cgtatttttc tacacaatgg atgatctcct ccacatacgt
     5641 ttcatcaata atttccttat cgatcaatac aagacagctt tcggcgatag cttcacgcca
     5701 atccgtgatt tctttatctg ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa
     5761 gaaactcccc ttctttctta taaaaacgac ggaaacggtg tatcattttc aatcgagaag
     5821 acatcttcaa atcctcgata aaaattaaac tcaagatcat ctttgtttgt cggatagatg
     5881 aatttcccgc ctacttgcca gatataaggt ttaaatccat acttcaggcg atcctttttg
     5941 aatttccaaa tttcagtgat ctcttttgga tcagccataa aattagacca aatatcgtag
     6001 tggacaggaa tcactacttt tgcattcaat gactccgcca tgcgcagcat atcaacagaa
     6061 gtaactttgt ctgttatccc tcgtgggttt tcaccatagg cccctaaaca gacatcgatt
     6121 ttgtgttcat taccatgttt ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa
     6181 agattccctc ctgatgtttc gaataaatag tttactgcaa tttgatccat ctcctgaggc
     6241 atttttcctt ttaatacctc ttctggatct tcacaagtga ccaacgctgt ccgatcaaac
     6301 gcttctaaag ctactacatt gatgtctttg attttgactg catcacctgg ctttactgta
     6361 atcgtttttt cttcaggaat tccccatttt tgccacgtgc gaaccacttc ttttggccca
     6421 atgaatgttg cttcaggaca attttgatga actgctgcgg ctgtatggat atctagatga
     6481 tcggaatgga tatgtgtaac gattaaagcg tctacatttt ttatcgcaaa aggatcgatt
     6541 acgaaaggct gagtacgtaa attaggctgc atattttgaa cgccactcat acgcatcatc
     6601 tgatggcctt ttttcatcat gccattccca tgactacgtt ttcccgtccc acaccataga
     6661 tcacataaaa tattcgttcc ttcatgactt ttcagccaaa ttcccgtaca gcccagccac
     6721 cacatagcaa aagttccagg atccactact tctgtttcga tttcttcatt caagtatgtg
     6781 ccccactcag gaaatgtttc tactaaccac ttttcttttg ttacttcatt gacagtttta
     6841 ctcatttgct tgctcccccc atatcgttgt tccaacttac aagtaaatat taatgtgtat
     6901 cagcatccat gaaaaccctt ttatatgacc gtttgttttt tatatgattt tctttcagta
     6961 atcaatgatt gtttcagtat aataaagaca gatatgaatg tttaaatgaa caactagtca
     7021 aataaatcca tggatcaaga tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc
     7081 aatattgaaa aacgtcaaaa agaaattttg gaactgcttc agcagactga acaactatca
     7141 actttcgaat tagctgaatc ccttgatgta tctattagta cgatacgaag agatttaaat
     7201 ttattagaag aaaaaaacga gatcatacgc aattacggtt attgttctta taattacaaa
     7261 aaccagactg attttgatca atctggtcct gaaaggatta aacaagcgat tgccagagaa
     7321 gctagtaaat acattaacga ttatgatacg ctgtttatca attctagctc gactgcgttg
     7381 aaaacactgg attttcttca atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc
     7441 aattactctc cgcacaaaga taattgtagt tatattttaa ctggtggaga gatgcgcttt
     7501 ccaaaagaag cactagtggg cgacatcgca ttaaatacga tttcttcagt caatgcagac
     7561 atttgtatca ttggctgtag cggagtcgat gtagtacatg gggtcacaac aaagatatta
     7621 aatgaatcaa cgatcaacga aatgatggtc aacaagacaa tcaaatgtaa gattttagtc
     7681 gctgatcatc gaaaaatcgg actgacatca aagttcaaga ttgctgatat ttcagtcttt
     7741 gattatttga tcactgataa attttgttct gaaaaagtat tagaggagat cagaaatacg
     7801 gggataaaaa ccattcaagt agattcattt taagaaccag gacgtaaata tgtaaatagg
     7861 attatcaaaa aaatgaataa caaatttatc gataaatgaa aaacacgcta cgctcaaatt
     7921 gataaaggaa tcccaagatg tatccatcca atcgatacca tcataatcta ttgcttcttt
     7981 tataaccgtt atatgttgcc taaataggaa tcaaacaata tcgaaaaaag gaggtatgac
     8041 aaaagtcaca cctccttttt tcgaataaac ggtattcaaa tcagaacact gatttcacaa
     8101 ccactttatt tcaggtcgtt ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc
     8161 gctatttttt ttgcgtccat atatctttct aaataatcat cttctcctaa ttgcttatgg
     8221 caagaagcaa tttcataaag caagtattcc aatgcataag tcgatttatg tttcttatta
     8281 tactcaatac ccttctcaca taattggatt gctttatcat aatcctcgat tttacggtaa
     8341 taggtggcga tatcataata tacaatgcag accatcagat tatcaaaatc agtctcactc
     8401 aatattttaa gtgtacgctc cacataaact ttcgcaaaat ctaattctcc ttttgacaag
     8461 tagatcattg ccacgtttgc agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt
     8521 tccaacactt gattaaaata aaataatgct gcatctggtt ttttcgcata aagatgatca
     8581 agcagtcctt gataaaataa aatttgattt aaaagatcag aattacttac tttttcctga
     8641 tcgatcgttt gtaaaatttt ataagattct tggtattttg atacagaatt caatgattga
     8701 atctcattta tttttttcct gattttgtct tcttctgttt ctactaatat atcggagatc
     8761 tcgacattca gctttttgca aagcgcactt aagatattga tgcctggcga catattcttg
     8821 ttttcgatat tgctgacagt tgcttgttca caaatgcctt ctgctaattg tacttgtgtt
     8881 aacccctttt tctttcggta ttcttttagt aatgctccat ctactttaat caaaacaatt
     8941 tccttccctt taaaaaatta tttctccttt atatcataca caaaaaaaag ttctaaacaa
     9001 ccaaaaatgt ttagaacaga ataaaaattt taattttaaa ataatattca aattaaacaa
     9061 ctggcgtatc tgctccttga tttctttttg tgttatctga ataattatcg tttacagtta
     9121 taacacatag tgaaaattgt catggcgaga aagtctaaga atatttttaa acataactat
     9181 ttctttctta cgtgtgtata atgacgacag cacacctatc aattaatata attattttat
     9241 ttttttatgt tctcatgttt atttttaatg acacaacttc tcgtatttat ctggcacaac
     9301 atataaatcg atcaaaaaat tgttgcgtgt ttttttgaaa attggagttt caacttgatt
     9361 acaaaattta aatttagaag ttgtgaaaac agcgagagac agtttaatat tcaaaatata
     9421 cataggctac tcgaacgctg tgttttttag tatactagta agaaacgaag ggatagactc
     9481 attccaataa ggaggatttc atgaagacaa ttctttttgc tcctgcagta ttcaatttag
     9541 ctgagacaac acgaatgatt gaggttgcca aagctatgaa agaccgttat tactgtgtat
     9601 tctttggctt ttctaaaact tttgctcctt tgatcacaga aagtggcttt gagtatcatt
     9661 tactagcgcc gatccttacg aaaaaacaag aagaacaaat cataaaattt gaccagttgc
     9721 gtacacttaa aaaccctttt accaaagaaa tggttgcctc tcgtatcact agtgaacaga
     9781 gtctgattgc taagtttgag cctcgactga ttgtgacagg ttcgaatgtg acaatttttt
     9841 tatcggcccg caataaaaag attcctttag tatatgttaa accctatgct ttgagtgaac
     9901 ctttttttca atcagagcat gcgctgatgc cccacgagtt acgtttcttc ggccctttaa
     9961 gcaatcccct ttggcactgg acaaaaaagc gtatagtaaa tacccgatgg atccccaatg
    10021 ccttcaaaca cgctgcacaa aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga
    10081 tggatgctga tctaaatctc atcacaacgc ctaaactatt tttggaagaa gaacagctgc
    10141 cggaaaatta tcgaatcgtt ggtccaattt ttgctaagct cagtacttcc ttgcctgaag
    10201 aagtgctaac ttttatacag cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa
    10261 tgggtagttc aggcaatccg cgtatgttac gcaaaatttt gttcttctta caaaaacaac
    10321 cggatctagc ggttattgca cctattgact atctgcttga tgacattcaa tctgactcag
    10381 aagatctttt aataacagaa tatctgtcta gtcatctttt gcatgaattc attgattttt
    10441 cttttatcca cggcggggaa gggaccgtcc aaactgcctg tgaatccggc aaaccgttta
    10501 tcggtgtggg gatgcaattt gagcaaagct gtaatattga ctattgtgta aaatacggaa
    10561 acgcaatcgc cctcacaaaa ccagtgactg aaagaaaact gcagattgct ctgcgcgaag
    10621 tttcttcacc caagattcgt cagcaggcac gcacgctgca agccgttttt ccaataaatg
    10681 gtccacaaca agcaattcaa gaaatcgaaa ccttcctgag caaccaccca atatagtttc
    10741 aagtagaaaa agctttttta atagagactc atcagtaatt tcgctattcg agacccagac
    10801 agtgaaaata caaaaaaagt aaatagtgga ttgaaaagtt agagttactt aaattacggt
    10861 gtctaaaaaa cacaaaatca tccttaaaat aaaagatctg acgctatcca aatgagcata
    10921 ggtagcgtct gccatttatt cttagtcaaa ttctttgaca cctttctctg gttggatcat
    10981 ctcaacgttt cttcaaaaga aagaccataa taattaggca aatacatttt cttaaaatgt
    11041 gtcatataga tctagaagca tcgctttgta gagatttgtg ccgctagtcc cctcaacaat
    11101 tactaaatca aatgtaggat ttaccacttt atctttgata atcaacctcc ctcttctgtt
    11161 gagacaatga tctcgcaatc cgatgcatac tcctatataa actgccagca atccccacat
    11221 attgagtaga tcatgtcaga atgttctatg tcgtcagcaa cgatacacta aaagtcacgt
    11281 tcaccatgac tgattgctat tcccagcaca attatttcta tacacgtttc atgatttgaa
    11341 taaacactca catcatacat attaccattc atacaccata ctgcagcccc tactatatgg
    11401 ttgtaagctc ccaaatctat atttgacttg atcactttgg tagtagatct actaattcta
    11461 aatccattgc tgataaaatt tccatttttt cctccttctt tctgccaaaa cagttaaaac
    11521 aataataatc tttatgtaaa aaagggctgt gacaaaagcc ttgtcacagt cccatattcc
    11581 gaataaacgg tg
//
LOCUS       DACENJ010000077        11282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.077, whole genome shotgun sequence.
ACCESSION   DACENJ010000077 DACENJ010000000
VERSION     DACENJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.077"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..958
                     /gene="manX"
                     /locus_tag="IW748_001473"
     CDS             <1..958
                     /gene="manX"
                     /locus_tag="IW748_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1104390.1"
                     /translation="FAEGILQSGSMIFGEQENVKAVILKPSEGPDDLRAKLEEAVASF
                     DNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAYASRFSMESAHE
                     IAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGDGKIKYVLARVD
                     SRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVKANVIPISKMIE
                     VAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKVVVSKVLSMGQE
                     DVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            991..1794
                     /locus_tag="IW748_001474"
     CDS             991..1794
                     /locus_tag="IW748_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1104391.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            1815..2729
                     /locus_tag="IW748_001475"
     CDS             1815..2729
                     /locus_tag="IW748_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1104392.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            2812..3186
                     /locus_tag="IW748_001476"
     CDS             2812..3186
                     /locus_tag="IW748_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1104393.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            3325..3570
                     /locus_tag="IW748_001477"
     CDS             3325..3570
                     /locus_tag="IW748_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1104394.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            3742..4806
                     /locus_tag="IW748_001478"
     CDS             3742..4806
                     /locus_tag="IW748_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1104395.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            5052..5477
                     /locus_tag="IW748_001479"
     CDS             5052..5477
                     /locus_tag="IW748_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1104396.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            5470..5919
                     /gene="nusB"
                     /locus_tag="IW748_001480"
     CDS             5470..5919
                     /gene="nusB"
                     /locus_tag="IW748_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1104397.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            6016..6870
                     /locus_tag="IW748_001481"
     CDS             6016..6870
                     /locus_tag="IW748_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1104398.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            6871..8208
                     /locus_tag="IW748_001482"
     CDS             6871..8208
                     /locus_tag="IW748_001482"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1104399.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            8213..8437
                     /locus_tag="IW748_001483"
     CDS             8213..8437
                     /locus_tag="IW748_001483"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1104400.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            8439..9329
                     /locus_tag="IW748_001484"
     CDS             8439..9329
                     /locus_tag="IW748_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1104401.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            9338..10156
                     /locus_tag="IW748_001485"
     CDS             9338..10156
                     /locus_tag="IW748_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1104402.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            10263..10712
                     /locus_tag="IW748_001486"
     CDS             10263..10712
                     /locus_tag="IW748_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1104403.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            10730..>11282
                     /locus_tag="IW748_001487"
     CDS             10730..>11282
                     /locus_tag="IW748_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1104404.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEK"
BASE COUNT         3683 a         1876 c         2583 g         3140 t
ORIGIN      
        1 atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag aaaacgtcaa
       61 agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat tggaagaagc
      121 ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat ggggcggaac
      181 accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg caatcgtcag
      241 cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa tggaatctgc
      301 tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag taaaaccgga
      361 agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac ctaaaggagc
      421 acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag cacgtgttga
      481 ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc aaccaaaccg
      541 tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac taatcgaaca
      601 agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga tcgaagtagc
      661 aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc ctgaagacgt
      721 attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt caatggcgca
      781 ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag acgttgaagc
      841 gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac ctaatgattc
      901 acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa aagcgtaata
      961 aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat tttagtcgta
     1021 ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt tcatcaacca
     1081 ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg tatcgtactt
     1141 ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc tgtagcacca
     1201 gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg acaaggtgtt
     1261 aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg gttgttctta
     1321 acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc ggctgctgaa
     1381 gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt acaaggggtt
     1441 cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt gcaatcattc
     1501 cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg aatggtcgta
     1561 gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg gccattcttc
     1621 gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct aggtgcttta
     1681 ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc atcaaataat
     1741 ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta ttaatcttga
     1801 aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat cgtttagctg
     1861 ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg caaaacggtg
     1921 gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa gaagaacgtt
     1981 ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt gcttctccaa
     2041 ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct gttgatgatg
     2101 tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt ggtgacccag
     2161 tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta gctatgggag
     2221 gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt tgggcattca
     2281 tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat gacctttctg
     2341 gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc gtattagctg
     2401 cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact gtaaaattag
     2461 ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga atcaagagtg
     2521 catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca acattacaag
     2581 acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg ttatgtatgt
     2641 ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc gtagtcggtg
     2701 ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg cgttgtcccg
     2761 agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa aatggttcaa
     2821 tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg tctgacagat
     2881 tacgggcaaa tcatgatagg tgataatggt tttgagtttt accatgcgcg agatagacgc
     2941 aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt catgctaaag
     3001 ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata cacattttct
     3061 tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga agatagaatg
     3121 gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat gtttaaacgc
     3181 aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta ctttgcagaa
     3241 gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat gctaaaattg
     3301 attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt tggagccgtg
     3361 atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg tgtaacgaat
     3421 atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga atggggacca
     3481 aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt ggtagcagtt
     3541 gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac taaaaggagt
     3601 tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag aagttttccg
     3661 gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa aaaagcgtgc
     3721 aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa aaaaatgcaa
     3781 gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta cttaacgaac
     3841 tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt tattactgat
     3901 tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat taaaaatgtc
     3961 ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca ctcattagct
     4021 tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat catcgatgcg
     4081 gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga tgaagaagaa
     4141 atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca catcctgaaa
     4201 atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt ttatatgcgt
     4261 tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt gcgttcggct
     4321 atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat cacattggac
     4381 tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc tattggtgat
     4441 cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt agcagttatt
     4501 gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag agattatatt
     4561 tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat cggtttagag
     4621 atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc aggaaatgtg
     4681 atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat tgaggatgac
     4741 ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga attgatcatc
     4801 ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga ctttgttata
     4861 gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa gagataataa
     4921 agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag ttttcttacc
     4981 acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta ggcaaaggga
     5041 ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa gatttaggag
     5101 agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct tctaaagtag
     5161 aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta ttaggacgtg
     5221 cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa gtagatcttt
     5281 actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg caggaacggg
     5341 tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat atccatgtag
     5401 tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt gaagacgaag
     5461 aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc attgcaggct
     5521 ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt caacgcaatc
     5581 gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc tgtttatctt
     5641 gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt gattgaaaaa
     5701 catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt gatcttgcgt
     5761 atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc tttaaatgaa
     5821 gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt caacggtgtt
     5881 ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt tttctatgaa
     5941 tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac acaaaaaata
     6001 tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga aaaaatgcag
     6061 gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc cggattagtc
     6121 gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa agaacgtgta
     6181 gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat ttctgaagac
     6241 gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg tatattggtc
     6301 caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat ccgccctgaa
     6361 aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg taaaccggac
     6421 aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc tatcgatccg
     6481 gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc gatggctcag
     6541 ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga aaatctggct
     6601 gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca ttttgtcaca
     6661 aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg tgatgctaac
     6721 ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg ctggatcact
     6781 cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca aaccgtaaaa
     6841 tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac tgtcactaca
     6901 ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg cgtatattta
     6961 acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt cagcttaaaa
     7021 gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa attacgattc
     7081 cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata tgaagcggga
     7141 ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc tttatatcaa
     7201 gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg gcctaaaaaa
     7261 atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg agcggttatc
     7321 cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt tttgttccca
     7381 acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca gagagtagaa
     7441 gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat tgaggattta
     7501 tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac gccggtgatt
     7561 tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga tgttcgggct
     7621 gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga gttgatgcgc
     7681 attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca gcgcaaacaa
     7741 gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag attgtatgaa
     7801 ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac acagcaaatt
     7861 tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca agtggctccg
     7921 ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg cttagaagag
     7981 aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt tcaatctctt
     8041 gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac gttaaatgat
     8101 caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat ccattatcaa
     8161 gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa atatggaaaa
     8221 tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt tagaacaagg
     8281 agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat taagcaaaca
     8341 ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga cagaatcaaa
     8401 tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact gacttttcag
     8461 ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag catacaacgg
     8521 atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa agaatacgtc
     8581 ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt cccgtctatc
     8641 aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat gatttgcctg
     8701 cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta ttcggcgaag
     8761 cattagctat attggccgga gatggtttgc ttacaggagc attccagcta atcagtatgg
     8821 cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt tgtgcgggat
     8881 cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa aagctttctt
     8941 tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt tatgccttgc
     9001 tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt caacgattag
     9061 ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg attggtacaa
     9121 cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat acttacccgc
     9181 gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta tccgccaaca
     9241 agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg atgcgccaga
     9301 tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac gcgtagatgt
     9361 attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt cagtgatggc
     9421 tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa aaattccagt
     9481 gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag ggggattaaa
     9541 attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat tgttagatat
     9601 tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa aaatgagtta
     9661 tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc ctagagtcgt
     9721 cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct taggaattcc
     9781 agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc ctccattaca
     9841 tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat tcgaagcagg
     9901 cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc aaaaagtttt
     9961 agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat taagttattc
    10021 tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga aagtacccga
    10081 aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg cacacgaaca
    10141 gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg gattcttctt
    10201 tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg agcgaagacg
    10261 taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag caagatatcc
    10321 gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt actcaagcta
    10381 cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca gatggcggct
    10441 accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg gaaaaactat
    10501 taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg aaaacattgc
    10561 cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa gaattatttt
    10621 cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac caggcagttt
    10681 tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga tgctacaaga
    10741 aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc acgaaggaat
    10801 gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg ctttaggtct
    10861 gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa aatgtgtgct
    10921 tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg ttgaattagg
    10981 gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa ctggaaaaaa
    11041 tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa tcggcagtta
    11101 tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg aatcacatct
    11161 tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga gttatcagca
    11221 agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc aaaatgaaaa
    11281 at
//
LOCUS       DACENJ010000078        11124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.078, whole genome shotgun sequence.
ACCESSION   DACENJ010000078 DACENJ010000000
VERSION     DACENJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.078"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(107..1984)
                     /gene="ltrA"
                     /locus_tag="IW748_001488"
     CDS             complement(107..1984)
                     /gene="ltrA"
                     /locus_tag="IW748_001488"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1104405.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            complement(2672..4366)
                     /locus_tag="IW748_001489"
     CDS             complement(2672..4366)
                     /locus_tag="IW748_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1104406.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLLKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            complement(4529..5122)
                     /locus_tag="IW748_001490"
     CDS             complement(4529..5122)
                     /locus_tag="IW748_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104407.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(5123..5455)
                     /locus_tag="IW748_001491"
     CDS             complement(5123..5455)
                     /locus_tag="IW748_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104408.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(5505..7877)
                     /locus_tag="IW748_001492"
     CDS             complement(5505..7877)
                     /locus_tag="IW748_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104409.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEIKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMKDSRLNQ
                     FKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLSESGETENASVQNIVTREAEP
                     KPIQNVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLRDIKQDIKP"
     gene            complement(7874..10483)
                     /locus_tag="IW748_001493"
     CDS             complement(7874..10483)
                     /locus_tag="IW748_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1104410.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            complement(10483..>11124)
                     /locus_tag="IW748_001494"
     CDS             complement(10483..>11124)
                     /locus_tag="IW748_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104411.1"
                     /translation="EYEIQQQELKEQEEKQRQKEKEREQLEIRKREQDLAEQKMAIEK
                     QRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPMNNQTNLTPSP
                     QRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEEAQEQARKEYE
                     QQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSESNEVK"
BASE COUNT         2953 a         2307 c         1789 g         4075 t
ORIGIN      
        1 tatctccgtc ttttccccca gtccacaccg tacgtgcgac tttcaccgca tacggcgttc
       61 cctcgtctat aaaagacatt ctgttttccc gtcctacaaa tatttattaa gatatttctt
      121 cgttaccaac ttttaatctc aactcattga gtttttcttt ttgttttgga tttaatttta
      181 gatattttag aagttcctca atcgtttcac tttgtgtagc gtgtattacc cgatgaacct
      241 cctcagtaac aatcactagg ttttcatagc tatcactttt gctttcatga taagggataa
      301 tatgatgaca atgcatttca gataagataa gctcctcacc tgttactgca catcttccct
      361 tttgagcgat gaattttgaa attcgattgt ccgcaaattc aatcgttgct ctcttactaa
      421 gcactggatt gctacacaac cacgatatct tccattcttc tacggtttgt tgcttcttat
      481 gaatggcttc cctaccttct tcggtgtatt tattaatatt tcttttttta cacatcggat
      541 gtctgtgttg aatataccct actggtaata ttggaaaccc tcttatatat cgtatttgtc
      601 ctgaccgtag gtaaggcttg tatccttcgt actttccgta ttgtccttct tttgagattc
      661 cttgttctcg tagtcgtatt tcaaaacttg gtctgagttc tcttgcaatt tttcgcattg
      721 ctacactaca atgagtcgca attttgaagt aattatgaat cccaataacc atactgttat
      781 agactccaat atagataaag gtatctttgc tattaggact tttttgaatt cgtttgattt
      841 gtttttttag tttcacttta atcatgcttt gtgcttttgt acctatatgc gtattaacaa
      901 cctttttgtt acctttggtc actaatttca tcgtaaagcc taaaaattca ctttcttctt
      961 ttgaaagatt tgtgattttt gacttttctt cagatattgg taactttaga cgctcctgta
     1021 accacatttt agttgcatag aaaacttttt tcgcttcatt ataatttcta cagaaaattt
     1081 taaaatcatc tgcgtatctc actaggtata tttcttttag agttgtactc tttctgagct
     1141 tgtgatagat attaccttta tcccaagctc catttttcct aaaatgatgt tttagctctt
     1201 tggcatcttt ttcctcccac tgatttgcta tccaccagtc aaattcgttg agattgatgt
     1261 tcgcaagtaa tgggctaagt attccgcctt gtggtgttcc ttttgttggg tatatcaact
     1321 tcccatttgg aagtactacg ggtgctctta acattttcct aatgattaag agaagctgtt
     1381 tgtctcgtat acccatagac cagatttgtc tcatgagttt agtatgattg acttcgtcga
     1441 agaaaccttt gatatctaca tctaccacat attccatgtg actttgattc attcgggcca
     1501 tacaatctgc tattgcgtgt tctgctgatc tatttgggcg gaaaccatag ctgtgattat
     1561 tgaattttgc ttcacatatt ggttctaata cttgtaagat gcattgctgc gcgattctat
     1621 cccatattga tggaataccc aacggtctta atttaccatt atatttagga atttctacac
     1681 gccttacttt ccgagggtta taatgtgcga atcgcttcct aacaatttct acgaattcat
     1741 tacggttgag attttcaata tctttaatcg ttactccatc ttctcctctt gtcacactac
     1801 cgtgatttct cttgatatta cgatatgcta ggacaatgtt gttatcagaa atgataatct
     1861 gcattagatt cttgaaatta tctcccttca aacttttgga gtatatctta tcgaagattt
     1921 ccgtcatgcc ataatattct gcatgccgca acttcctatc ttttggactt gtttcttttg
     1981 tcatggttta tcacgctcca attggctcgt gaccttttta gtcatacgag aatcccatgg
     2041 ttgaacgggt ttcatgactt ttacagacga atcgagcctg ttcctccagt tccattacag
     2101 aaccttcatc agtcgtgact ctactttcac catcactaaa gtaagttatt tgtcattcac
     2161 atatttctta ctaccacttc atcgagtatg cactctccat gttgatggct tcccctgttc
     2221 ccttatatct atccacatgc cttaacttag atgctagctt ttgcccccta acttctccta
     2281 cgcctgtaac gcagcatggt ctttcataac aacgactttt actcaaccac aagttagcac
     2341 aatgagtgat tttttgctca ctgtctctcc catttctagg agctatgcct ttcggacagt
     2401 acattcgcta gttcgtcagt acgtttgtac atactcacca tattaagttt ttttggacac
     2461 ccgacttatc ggatacaccc tttacctcta aagaactttc gtggtattca ctatttcttt
     2521 taccttacgg tatccttcag ccgacttcac cgagcttcac acaacgttgg tctgcataaa
     2581 ctacttgttg catgtcggag tatcagtgga agccctttgt ggcgttaccc actcatttga
     2641 cttctcgtta taagagttat tacaatagtt tttagactac ttgcaaccca cagcttttca
     2701 ctgcagaaca ggtcgcacca ttatggcact catcaatcat cgccaagaag cgtttgaagt
     2761 cttgttcttc catttgtttt tctttaagta aacgtcgttg tcttcttcct tgaatcaatg
     2821 cctgattcca aatcaacgtc atagccatgt acatttgaca ttgttgtacc cctcgatcta
     2881 agcctgtaat agagtcaatg tcaaaaaaga ccaactgttg ttggtcaagg tgtcgaatcg
     2941 tactgtgacc atcaaaaatc atgccatacg tctcacataa ggattctacg atggattcca
     3001 tttcttcttt tcgattcttt ttctcttggg aagcacctgg taattccagt tgtcctaaaa
     3061 agtctaaaaa ttctttgaac gtcggataag cttctggatc tttccctgtg acttgcgtct
     3121 cagtatttga acgcgaattt gaggaagtaa agccgcgtgg tactgttcca tactcttcat
     3181 aaaaacgatt taagtaggtc ttaaattctg ctttcatcgt tccttttagc tgaggactca
     3241 ctaactgtac ctgatagact actttgtcta agtgtgtttg ataggacgct gcttcattga
     3301 tcttcaaatc tttgactgat ccatcgtcat ataaactgga tgcttcatca tacgtccgtg
     3361 tggcaaatat ttctaaagga ttgatcatcc cgtcagaccc gtctaagcgc accataatgc
     3421 cgttatattc ctttaagaag gggataaagt ctttcccctt ttcaaagccc caaataaagt
     3481 ctcctgcagc taaatgggct tccgcgatca ttttaagaac ggttgattta ccagctccca
     3541 tcattcctag aagcaaggta ttagtagaca accgcacact atttttatat gtcgcattcc
     3601 agacaaatgg gccacctgtc atggtttccc catattgagt ccctcctgga tcttttagcg
     3661 tttggtggtg gaacgggaat cctccgccaa tatttaacga tggtagtgat tgccctttga
     3721 tatttccaaa ccattcagct tgttcatcga acatggtaaa catcgattgc caatctcttt
     3781 tcgttttgtg cagataggtc actgcacgat aatccatccc ttttaaattt tcaatcagtt
     3841 cctgcgctcg ttcatctact tcttgaatcg tgtttccgta aacatgtact ctcactttga
     3901 cttgcttaga aatttcccca ttagaagtca atttacgagc atattccgct agttccaggt
     3961 attcttctgc gcttttattt cggtcagtcg cttttcgttc attatctgat cgatcattta
     4021 attcatctaa ggcacgattg atttgatcaa tcactttttg cttttcatct gtgctaacat
     4081 cgagtgtggc cactgtatga ggattgttca tgagattggc caaccaaaaa agcgtaggat
     4141 tttttgtgta gtgataaact gtcacacatc gtgaataacc attaccatac gtcacatatt
     4201 cttcatcaaa atttaaccca ccttgtggca tcacttgttt taaaaaatct aaatcatacc
     4261 ctttggattt caaaatttct atttcccgat ttcctagttg ctttaatgga tcaagtactt
     4321 tttgatcatt ttgtggtgaa aaagctgttt taattgcttc taacatgtac agtcacctct
     4381 tcttgtttga ttctttactt ggttaaaaga ttgccctttt cccaacttac ccaggcttaa
     4441 agtgtgagga aattttctag tggtcccttc cttcggaaca tctttttcta accatatcaa
     4501 tggatatttc attctctctt ctccattcct actcatcatt catattgttc attcgataca
     4561 gaatcttgat caattctttt ttgtagagtg gttttaaccc taaaatgcca ctagatcgca
     4621 ttaactgacg gtttctggtt tgtaaatcct tgattgtctt tccttcaatg acgaaaaaga
     4681 agcttaagtc aggacgttct tgttctgctc gtttcaattc ctggatttct tcgactaatt
     4741 tcttttcata tttttttaat cgttgtagat ttcttcctaa ggattgttcc atacgattct
     4801 tatctaataa ctgccgcttt tgcacgattt gttccgttag atccgtctct gtagtcaaac
     4861 taactaactt aattgttggc acatacgtac gcatgacggt ggtcaactga ttcattcgtt
     4921 ccagtttctc ttgttcattt aatccctcca cgtcagtgga aacgacacga taataacgtt
     4981 gaaaatgatc ttctttagta actaagactc cctcttctgt acaggattta taaggaaata
     5041 aatccaagat agatcgttct tctggttttc caaactgcaa aatagatttc ttatcaactg
     5101 aattttgtcc tttgactcgc atttattcat cctccttata cgtaccatga taatttcggt
     5161 taggatctaa cgcatgataa tggttcttgt ccgcaaaaaa aatagactcg ataatcgcaa
     5221 aaatttgtcg ttgacctggg ttattatagg gtcgccaaac aatcactaac gctatcgcaa
     5281 tccataagcc ataattcaag agtgcaaaaa agagtggtaa gtggacaaga ttattgacca
     5341 ttttcgccca aaaaaagcag ccaccaagcg ttaatagatc taaaaagaaa aaagcgaaaa
     5401 acttccattc tccactaaat cttggagggt ttttcatgtt tatatattta tccataattg
     5461 atttctcctt ttcatataaa gaaaaggcct aacttgtagg ccttttatgg tttgatatcc
     5521 tgtttgatat ccctcaatag ttgttctttc aatgcattgt tcctgttttt ggtatctctt
     5581 gtcacattta tttttgtcgg tgcagtaaat gccacatttg ctggcttgta acctgtatga
     5641 acgttttgaa taggttttgg ttcagcttct cttgtcacaa tattttgaac ggacgcattc
     5701 tctgtctcac cactttcaga taaattgtaa gaaacagttg tcaaattccc attttcaggc
     5761 aaacgacttg atttcatttg aacagtacct gcaggtgatt gatgaacaaa gctttgatca
     5821 ttctctttga attgattcaa acgactatct ttcattggta aattttgtgt cttaggcaaa
     5881 ccaattttcg ttgatttttc agttccaact tcgatgccat tatcatttgg cagatcgatg
     5941 gaatcatcat ccgtttgtac ttcttgattt cgttttggta aatctgaaat gcgcttttca
     6001 ttttcagaat taatgttaga tttataacga tattgttctg gatctgtttt tggtaattga
     6061 ccattttgtc cttcttctga attttcgtta gaatgcaact catcttggtg atctttggct
     6121 gaaggaatcg ctgcaccttc ttgattttct ttttcggttg ttgtattagc ttttactgct
     6181 gcattgactt gtccttcacc atacgcatct tttagtcctt ctttgatttc tgaagctttt
     6241 tctttttgtt tttctagtgt ttcggatgga ttcttcacag cttcagccaa ttctttagct
     6301 ctcgtgccgt ctaatttctc ttctaaggtg tctttggctt tttctgtaag atcttcagct
     6361 gttcctttta acccacgttc attgacgtaa ccaagacctt ctgcggcttt agaaacacca
     6421 tttttcgctt tcccgaacat ttcggaaact gttttcttac tattaggtac agttgtagct
     6481 ttatcgtctg attgttcttc cccgttatca ggggtaacat cttttcgatt accaaatcca
     6541 tttttacgaa tatcatttaa attgcttaaa ccactcttac cagcacttgc aaggttttta
     6601 gccccgctac ccacattttt cgcaacattt cctaaggcaa acatgctaag aagagaattg
     6661 ctgccgtttt tcagactcgt atcaacgccg aaaattttcg taactgattg agagcctttg
     6721 aatagcgcaa tggttaatac aattaaagcg acaacataca agaatccatt gatctttcct
     6781 gccgcatgca aatcaccgat acctgaaagc attaatgtat agattcgtaa ttctaaaatc
     6841 gtaaatgcca ataacaagaa ggattgagaa atatcttcta tagcactttt catccgttgt
     6901 cctgtctcaa agtccgttga cgcggcaatc actaaataga attttttaaa taccagctca
     6961 ataatgcaag taacaatcgt aaacaagata aataaatagg ctaccgacaa agaagattca
     7021 ccagctaaaa taatacctgg actactaatg tatcggcgat agcctgaagt atacacatat
     7081 tttgctaaac cttcatcact aatttgaaca gggatttctt tcccattatc gtctaactct
     7141 agtcgatact gtaatgcctt taaccgatct ttttgtactg tactaatatc acttgattca
     7201 gacattttct tagcttgatc aggtgtaata atcccattga tattcgctgt tttaaaattt
     7261 ttcgccgtga gagcatttct tttgtctgtt ggtatgtgtt ctactttgtt cggatcattt
     7321 tctagtaacg tactcacatc aatcacatta ttttgaataa tttgaaaagc aggtgaacca
     7381 gttttatctg ctttatttgc tgcagtcacg tctccgtaaa agtttttact aatatttagc
     7441 ccttcatcga ttaaagaact accaccaaaa atcaaaatca tggccataaa tagattaacc
     7501 aagatatttt tgatttttgg tggatgttta ctcataaaaa attttaagcc taaataaatt
     7561 aatgtgacga cacaaaaaag agccaatacc ttactaatca aactttgaaa cagttcatta
     7621 acaccagcgg cgttcaataa gtcttttaaa tcaaagcttt gataaattgt tttttctagt
     7681 aaagtattga gattatataa ccctttaatc aaaccccata aaaaagtatt acaaatccaa
     7741 ctgaatacat cttgatactg cagataatta ccataatctt gtaagacttt cgttacggtt
     7801 ttgggatctg catccccatc cccaaataag cctgaccacc agtcggcaat gccggaaaat
     7861 attcctccaa tgatcattct ttcaccgcct tttcagcttc tagcaaaagt cgtttcctta
     7921 taacctctgt aatttcttca ttttgacgca atttttcaag gtattcttgc aaacgatcta
     7981 tttgcttctc ttcataaaat tgaataagta acttttgtgc cgttgagttg tctaaagatt
     8041 gaacgatttt aataaaaatg ttttctttag cttcctcttc tatttgatcg tgcatattgt
     8101 gataaagtcg acgtgcatag atatcaggta gaaaataatc gtaatcaatt tgtaaagcct
     8161 taagatccaa gtccgcatgt gtacaatgta tgtctatatc attgatatcc cacgatggat
     8221 tgaaatcttc aatatattga taagcataag gaagattcgt ttccccatga ttaaaaatag
     8281 gatacggccg tattttatga cctttcaaat cttgtcgttt taaaggagcg agtacaatcg
     8341 tttcaccttc taaaaaagta gaaattcttt caggagttaa gatgcgatct gaatcaacat
     8401 tgcgattgat atgagtattc atacctaatg aattgatggt actgttctct tgttcaaccg
     8461 tcttattccc cacttttttg gaaaattctt ctatggtatt atcgtctgtc gacataatat
     8521 aaatatgtgt ctgacaattt tctttgataa tcgctgcgtt ttgttcccca tacttcgcat
     8581 aaagttgctg ataggattgg ataaacaaat cccacaacat gtttctacca gctgtgactg
     8641 tcatgattcc ttccatatta tccattggtg gcatattccc aaattcatct aaaataaaat
     8701 gaatcctacg gtgacattta tctcctttag tcttaacaca ttgcgtagat aattctttat
     8761 acaactgact aagaaaaata ctaacaatga tatggttaga tggatcataa tcaggtgtga
     8821 gcataaaaat agccattggt ttatcggtat agtgcatccg aatagactct actaataagt
     8881 tacttttatc ctcatctaat gttagaacgg cttctttttc taattctggc aaatgggttt
     8941 ctcctcgttt ttttcgataa acgatatttc cctcttcgtc tttcaatact ctttgagaaa
     9001 actcatacca agcaacatat tcttttcctc gatgatcgta ttttaagcaa acatgatctc
     9061 cctgttctaa atgacaatca aagttaattt cacaaaaacc tctatagcct actttgacac
     9121 gttcctcatg aaattttttt ccttgatgat aaaactctac aacaattcgc tggtttaaca
     9181 gactctcgtg tagtttgaga tccaaatact tcggaaaacc aatggactta taatcaatgg
     9241 ttgattgaga agtcattttc gcaattttcg tcagtgtaaa tggatttagt cctgtgatcg
     9301 ctgtcgatag aatgttgcct ttcgctttat cagaaccaat ttttgttgcc ccatattgtt
     9361 tttttgctat gtttccaggt ggtaactgat tcataaattc atctaaagca ttgagctgct
     9421 tgaattcatt atcaggatct tggtaattaa aaccaccaat ctcagaaaga agttcgacga
     9481 gattattcgg tgttaatttg gagtaatccc ctgtctgatc cgcttgttcc aataacgcaa
     9541 aaataagcgc attgacagct ttttgcgcat tttcgttaaa gtatttattg tctgaagcgt
     9601 tggcatcata ataaatcgta ttcgtgaaag tattggttaa ctgtgtcgct gtgtctatgt
     9661 ctcctcgctt ccagtatttc acgatgagtg ccaatggatt atcactcatt gattgcagtg
     9721 gatcggtgat attaaatgca tacacatcat accctctagc ttctagttca tctttactag
     9781 caacatataa ttctgttttt ggatcattaa caaccattga actttgtctt tctgcacgag
     9841 acaaattgtc aatggttgct aaaacttcta tctgtccttt ccctgaacgg gaagtaccaa
     9901 caatacaagt atttactgta tccgtctgaa tataccagta ctttttataa tgagtaattg
     9961 gtattccgcc ataaccagga aaacctccct ttgattctgg tatttgcgga taaatttgtt
    10021 ggatttcctt taacgttttt aatcggttat ctcctttttg cccatctgct agaggacgaa
    10081 accacatgtc cattttcaat cccataagta cagctggaac aacacaaatc gtagaaacaa
    10141 ctaaacgtgt gcctaaactt gttgttgggg tcaaaggatt ccaaaaacca gttgaatcaa
    10201 aatggaataa ctgcggcatt tcttgtaact gaaaaatatt cactccttgt aaacttcctg
    10261 gaataatctc aaatcgatga aatgcttctg gtattcttgt aacaacatag tttaaaggaa
    10321 gataagctaa gccactataa aaaatctttt gagaaatacg tttgtcatgt ttttctggta
    10381 gctcttctcc tgaaggagta tagcgataga aatgagaaag atacctattc ttttctaaat
    10441 tgaatcgttt gtcttttcgt ctcgttgttt ctctaacacg cattatttca cctcatttga
    10501 ttctgaactt tcggaagtgt ttgattgtac agttgaatcg cttgatcctt ctttagcctt
    10561 actaggatca ctctctttgc tattttccat acttgattct gttgttgact gattggtacg
    10621 attcaataat tcttctaatt gttgttcata ctcttttctt gcttgttctt gggcttcttc
    10681 cttgattgac gtcgttgtgg ccacagaatc agcctgtgaa gattgattga tatgattaaa
    10741 agcaaatact gttcctccac ctagaagcaa gcttaaactt gcaatacatc ccaccatcca
    10801 tccgttaaag gaacgctgtg gtgaaggagt tagatttgtt tggttgttca ttggggtttg
    10861 aacagcagct actgaaggcg ttggttgagg ttgtgctaga taatttaact gagcttgtcg
    10921 caatgtatcc atttgttctt gacgttcctt acgagcagct gcttcttttt ccttttcttg
    10981 ctctaaccgt tgtttttcta tcgccatttt ttgttccgca agatcctgtt cccgttttct
    11041 tatttctaat tgttcacgct ctttttcttt ttgccgttgt ttttcttctt gttctttcaa
    11101 ttcttgttgt tgaatttcat actc
//
LOCUS       DACENJ010000079        11070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.079, whole genome shotgun sequence.
ACCESSION   DACENJ010000079 DACENJ010000000
VERSION     DACENJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.079"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            51..1421
                     /locus_tag="IW748_001495"
     CDS             51..1421
                     /locus_tag="IW748_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1104412.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(1476..2372)
                     /locus_tag="IW748_001496"
     CDS             complement(1476..2372)
                     /locus_tag="IW748_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1104413.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(2653..3330)
                     /locus_tag="IW748_001497"
     CDS             complement(2653..3330)
                     /locus_tag="IW748_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1104414.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(3421..4326)
                     /locus_tag="IW748_001498"
     CDS             complement(3421..4326)
                     /locus_tag="IW748_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1104415.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTTVSWTT"
     gene            4501..5955
                     /locus_tag="IW748_001499"
     CDS             4501..5955
                     /locus_tag="IW748_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1104416.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            5971..7002
                     /locus_tag="IW748_001500"
     CDS             5971..7002
                     /locus_tag="IW748_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1104417.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            7009..8196
                     /locus_tag="IW748_001501"
     CDS             7009..8196
                     /locus_tag="IW748_001501"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1104418.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            8352..9734
                     /locus_tag="IW748_001502"
     CDS             8352..9734
                     /locus_tag="IW748_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1104419.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            9706..10092
                     /locus_tag="IW748_001503"
     CDS             9706..10092
                     /locus_tag="IW748_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104420.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(10292..10960)
                     /locus_tag="IW748_001504"
     CDS             complement(10292..10960)
                     /locus_tag="IW748_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1104421.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         3210 a         2168 c         2178 g         3514 t
ORIGIN      
        1 aacgtttgat tattttttct tgttgactca acaacgaaag gagtttttgt atggaagaag
       61 aaaagttaac tcgaagtctt agtgcccgtc atatccaaat gatcgcacta ggtggaacga
      121 tcggtgtcgg tctgtttatg ggtgcctcct caaccatacg ttggacaggg ccctcagtga
      181 tgggagccta tgcgattgca ggattctttt tgtatttgat tatgcgtgct ttaggcgaaa
      241 tgctttacgt tgatccttcg actggttctt ttgcaaatta cgcgagcgag tacatccatc
      301 ctgtggctgg ctacttgacc gcttggagta atatttttca atatattgtc gtaggaataa
      361 gtgaagtgat tgctgtagga tcttatatga attattggtg gcctgatttg ccggcaatta
      421 ttcccggaat cattgtcgtc ttgtttctta tgctggcaaa tcttatttct gtcaaggcgt
      481 ttggcgaatt agaattttgg ttttcgatca tcaaagtcat aacgatcatt ttgatgatca
      541 ttgcaggtct tggggtaatc ttatttggat ttggtaatca tggtcaggca gtagggattt
      601 caaatctttg gaaaaatggt ggattcttca ctggaggcgt caaaggattt ttctttgcat
      661 tatcgatcgt tgttgcttcc tatcaaggaa tcgaactgat cggaatgact gctggtgaag
      721 cagagaatcc taaaaagacg atcattgaag ctgtccagtc cactatcggt cgaatcttga
      781 ttttttatat tggtgctatt tttatcattg tgagtatcta tccgtggaat caattgagtg
      841 agattggttc accttttgta caaacttttt ctaaagtcgg cattacattt gctgcaggac
      901 tgattaattt tgtcgtgatt acagcggcat tatcaggttg caattcaggg attttcagcg
      961 caagtcgtat ggtctataca cttgccataa atggaaagat gtctaaaaaa tttctgaaac
     1021 tatctcgtca cggtgcgcct ttttatcctg tcgtagcaat ctcaggtgga atcttaattg
     1081 gtctgatctt aaattatctt ctaccaatgt tcatcaacca gtcagaaaat attttcgttt
     1141 tcgtatacag ctcaagtatt ttgcctggta tggttccttg ggtagtgatt ttgatcagcg
     1201 agattcgctt caaaaagaag cacgcagata aaatgcatga tcatcctttt aaaatgccac
     1261 tatctcctta tacgaattat gtgacacttg cttttttagc tatcgtattg attttcatgt
     1321 ttatcaatcc tgaaacgaga atctcattga ttattggact cgttttcttg ctttacatga
     1381 cgatccatta ctttgtacgc gaaaaaagaa ccggaaaata aaaaaagaac gaagcttcac
     1441 cttagtggaa gcttcgttct tttttattgc ttttatcaat tcaattttag cgtctcaccg
     1501 ggagtcagga aataattctc catgctcatc ccattcagcg acaagatcgt ttctactggt
     1561 acgccagttc gttcggctat ttgatttgga ccttctcctt ccaaaactac ggttgtcgca
     1621 gcctgtgaag tcgcgctgtt ggtattagtt tcagaggaat ctgttgtgtc ttgtgttgtt
     1681 cgagcttgca tttcagaagc ttctaccgac tctgctgtct cagaatcaga agatgttgtg
     1741 tcttgtgtgc ttgttgactc tgcttgaaga gaggcgctag tctgcaaata aatatcggaa
     1801 atcttagcga catgattcgt ataattagcc gataattgat tgatcaaagt atctaagtct
     1861 acaccgtctt tattttctgc tgcctgatac gctgcacgta attttctgat cccttcgtcg
     1921 gagattgctt gaagctcacg tgctttttgg ttagcaactg ctgataaacc agctattccc
     1981 tcttgatttc cctgaatttc tgtctggtat tcttcaatca atgcaggcgt cttttcttcg
     2041 atctttttcg aataatcatt tacgattgct tgaagttgct ctggcaaacc atttgctgtt
     2101 ttttcttcag aagtttgtgt ctcttttgtt ttagttgatg actcacttgt ttgtttcgtc
     2161 tctgttttct ttgtcgttgt ttccgttatg gtcgtacttt tcgcttctga cgaatttttt
     2221 ccatgaaaat atagacccgc aactcctcct gctatgatca aaaacacggc aactgctgcc
     2281 ccaatcatga tcatccgctt acgagctgcc tgtttttgct gtcttcttcg ggtcatccga
     2341 acattttctc cccactcatt ttcatcatac atttttttca ccaccaattt ttatgtataa
     2401 tagcgttatc aaacgcttca tattcttatt gtagcaaata attgtcgctg tgcacatttt
     2461 tatctttgtt atttataaga aaatttcacc agttgagatg attccaagat tgtccgtcga
     2521 tttctccttg cagctgcatt gttcgttcgt ctatctcagc catcagtttc tttatttcat
     2581 ataagagcgc acggtcttga tattctgatt tttcgcttaa ttcatctaat tgttggatca
     2641 gccaatcatt tgtcataatg aagttccttt catttcttgc agtgtaaagg tcaagtcttc
     2701 gatctgctct tttgcctcag tcagtgcttc tacgatatat gcttgttttt cagattcttc
     2761 ttcctgcatt ttctgatttt cctgtactct ttgaataaac cagtcaatcc gctgagtcag
     2821 cgatggtttg ccttccaatg agagatactg ctgataagca attagcttct cccaacgttt
     2881 ttcctgggat aaatacataa attgtgctaa aacgaatggc ggaagatagg tcatatggaa
     2941 tttatttgcg attcgctgat acggcctcaa aaattcggaa atcgtatatt cttcttttcc
     3001 tccagcttgg tattcttttt ccgccacacc tgttgtcaat acgattccta gttctttccc
     3061 ttttaataca tgtgctgctc gttccaagac attatcttgc caaattttta aatgagcagg
     3121 agaactatac cagtaaaacg gaaactggaa aatgatccga tcatgctggg ccaagagctg
     3181 tagttcagtc tctatatcta tttttccgtc tggataactt gattccaaat gatgccaagt
     3241 agctggtaaa ccagcagaag cttctttgaa aaattgctga tttaaagatt cttcgatcgt
     3301 tggatggcta atgatgataa gtgtacgcat cccttttcct cctttttagt cttcgcttta
     3361 cagtgtagca taaccgccac aagtttcttt tgccgatgct tgtcatttta accagactaa
     3421 tcaggttgtc caagaaactg tggtttgttt ttccaactgt tttttcaagg tatccaaagc
     3481 aatattctta aaatttttgt ttttcatacg tttacgatag ttcgtagccg tttctccggt
     3541 actctttttg aacattcggc taaatacgac tagactattg aaccctactt gataggcaat
     3601 actttccacg gattcatccg tcagagaaag aagaaacgcc cctcgattga tccgtacccc
     3661 gatcaaatat tccttgatac tggtacccat atgatttttg aatactcgcg acaaatggct
     3721 ccgattgacc gctaaatgct gggcgatttc ctcaacagac aatttttcag cataatgtgc
     3781 ctcgatgtat ttgactgttt caacagctaa agaggaatga accttttgta caccaccgct
     3841 gacctttccg ccatctttta aaaggactga taaaaaacgg taggttaatg ctgttaactg
     3901 taattcattt tccaaggttt cttccgtatc tttcatgcat tctaaaataa tgtcgatata
     3961 tttttgtatg tcagaagaga ccatcgtgta ttggtcttct ctgaaaagcg actgccgaat
     4021 gatttcttct gcagcactcc caccaaatga aagccagcaa tacatccacg gatcttctcc
     4081 atctgataag taaaacgtcg agtctcctgg acgaatcaag aacagatcac cttttttcaa
     4141 ttggaattgt tgatttttca catgataaga tcctttgcct tccatcacga catgcaggat
     4201 gtaatcattg cgaatagcag gaccaaaaga atgaaacggc atcgttttag agaaaccaca
     4261 aaaatcaaaa tacaattctt tttctacttg ttgatgttcc ttttttaaaa aaatcgattg
     4321 ctccacgtct atccctcctt actcctttag tataatgcaa caaaaagtta aataaaagtc
     4381 acagaaatat attaaaaagg aagcgatttc ttgttaagtt taagtcatta aaagatcttt
     4441 gttattcgtt gtaaccggtt tacttagtat tatatattga acaggagagg aatacagatt
     4501 atgaaaagca aaatgatcca acgtgtgtcc tacgctttag gcgcttttgg acacgacaca
     4561 ttctatgtta cattaagcac ttattttatg gtattcgtga caagccagat gttcgcagga
     4621 acagataagg caacgaatgc aaaaatgatt gctacagtta caagcttagt tgtaggtatc
     4681 cgcttagttg aaattatttt tgatcctttg attggtggca tcatcgataa tactcgtaca
     4741 aaatacggta aattcaaacc atggttagtt atcggcggcg ttgttagttc cgtactcttg
     4801 gtagcaatct ttaccaattt ctttggctta gctactcata atacgacttt atttatcata
     4861 ttatttgcaa tcgttttcat cttattggat agtttttatt catttaagga tatcgctttt
     4921 tggtcaatga ttccagcttt gagtacggaa acaaaggaac gtgaaaaatt tgctacgttt
     4981 gctcgtttcg gctcttctct aggtgcaaat ggtactactc tagtcgttgt ccctatcgtt
     5041 actttcttta cctatcttgt tactcataaa catattgaag gcgcttcagg ctggttctgg
     5101 ttcggtgtga ttgctgctgt tatttcaggc ggtactgcga ttatcactgc cttgggcaca
     5161 aaagaagtcg atactgcgat ccgtcagcaa aatgaaaaag ctgggatcaa agatgtgttc
     5221 aaagcagttg ctcaaaatga tcagttgatg tggcttgccc ttgcttatat cgcttatgcg
     5281 attgccaatg cagcaacaac tgccgtatta ttctacttct tcaaatatgt acttggccaa
     5341 ccaactgaat tttggatcgt tggtctagtt gctgccttac ttggtttagt agctgttcca
     5401 ctattcccaa tgcttacacg cgtcatctct cgtcgctacg tttaccttgg agggatcgca
     5461 atgatgattt cttcttactt tttctttacg atcgcaggtt caagtttgcc aatcgttatc
     5521 atcgggctag tattgttcta cttccctcaa caattgattt tcttatccgc tttgatgacg
     5581 atcacagact ctgttgaata tggtcaatgg aaaaatggta tccgtaacga agctgttact
     5641 ctatcaattc gccctttact agataaaatc gctggtgctt tgtctaacgg gatcgtaggg
     5701 tttgtcgcta tcgcttctgg gatgactggt aatgctactg ctgctgatat cactgctcat
     5761 ggcgtaacga ctttcaaagc ttatgcattc tatgctcctg ctgctttgat gattttctcc
     5821 gctattattt ttgcttggaa gattacattg acagagaaaa aacatgcaca aatcgttgag
     5881 aaattggaaa acaaattggc tccagctgat acaatggaag atgaacttca cgatgcagta
     5941 gatccttccc attaagaaag gatcttgaaa atgaagatta cagaaaaaga ttttggccaa
     6001 ggttatcact tgataacctt ggagaacaaa aacaagctag cactttctat ctctgattta
     6061 ggtgctagaa tcgtcagttt aaaatcaaat gatcgagaat tagttttggg atttgacacc
     6121 gctgaagaat atatcgaaaa agatccgtat atcggtgctt ctatcggtcg caccgcaggg
     6181 agaatcgaaa atggccactt ttctctcaat ggaaaaactt accaattagc cacagatcca
     6241 aaaactggac ataatctgca tggtggtgct cctggatttg aattaaaaaa atggtcttat
     6301 gtcattttga atggcgaaaa tgaagcttct gtcattttta ctactacaag tcccgacgga
     6361 gaacatggtt ttccaggtac aatggatgtg gaaatccgct atactttgac aaaagataat
     6421 atctggcgag tgacctctcg cggcaccagt gaccaagata ctttattcaa tccgacaaat
     6481 catgtttact tcaacttgac gggagatgct tctcagtcaa tcgatcaaca tgagctttgg
     6541 ctcaatagcg aagcttatgc tcctttgcga acagatagta ttcctatagg ggtaaaagaa
     6601 aatgctgcag gaagtgcttt cgattttcaa atacctaaaa aattagcgtc cgtttttgct
     6661 agtgatttgg atcaaaaaaa tcttgttgac ggaatcgatc atccattttt cttaaaagaa
     6721 accggcttgg gtaaagaagc cgctcgcttg actagccccg ataaaagaat ccaagtagat
     6781 attgctacag atgcttctag tgtggttatt ttcactgcaa atttcggcac agaaacacca
     6841 gaaatgcgca accgaaaact ggcacatcac ggtggcatca catttgagac tcaaacagct
     6901 ccaggtgcag agcgcttctc agcatttggt tcgattcatt taaaagccgg atcagtattt
     6961 gaaacagtga cagaattcaa aatcaaaaca agaaaggaat aactcatcat ggaaatcaaa
     7021 agaagcttaa acacaaaatt tgccgaaaca ttcggaccta aaaaaactac acaatacttc
     7081 tcaccaggac gcatcaattt gattggtgaa cacacagatt acaatggagg ccatgtcttc
     7141 cccgcttcca tcacttatgg aacgcaaggt gtagcagcac ctcgagacga taataaaata
     7201 ttagtttatt ccacaaactt tgacgatatc ggtgtcatta gttttacttt agatgagcta
     7261 gaatatgata aaaaagcaaa ctgggcaaat tacgtaaaag gaatggtcct taaattaaaa
     7321 gaagctggac actcaatcga tcatgggttt gagttattag tggaaggaac aatccctaat
     7381 ggtgcaggac tttccagtag cgcttcatta gaattattag taggtgttgt attagaagat
     7441 ctgtttgatt taaaaacaga tcgcttggaa ctagtccaaa caggtaaaaa agtagaaaat
     7501 gaattcatcg gtgtcaattc aggtatcatg gatcagtttg ccatcggctt cggtgagcta
     7561 gatcatgcaa tcttattaga tacaaatacg ctgaaatacg aaatggttcc agtgaaacta
     7621 gatggttatg caatcgtcat catgaatacg aataagcggc gggaattggc agattcaaaa
     7681 tacaatgaac gccgtagcga atgcgaagaa gccttggcac gtttgcaaaa aactttgcct
     7741 atctcttctt taggagactt agatgaagaa gaatttgaat caactattga ccaaatcggc
     7801 gatgatacat tgatacgcag agctcgccat gccgtttctg aaaatcagcg gactttgaag
     7861 gcaaaagctg aattagaagc tgggaactta gaagcatttg gtcaactgtt aaatgattct
     7921 caccactctc ttcgctatga ttatgaagta actgggatcg aattagatac actagtagat
     7981 gctgctcaaa aacaagaagg tgtcttaggt gcacgaatga caggagctgg atttggtggc
     8041 tgtgcgattg ctcttgtcaa agaagaaaat atacctgcct tcaaaaacaa tgtctatgat
     8101 gaatacatga aagttgtcgg ctatgctccg gaattttatg ttgcacacat tggaaatggg
     8161 acaacaaaga tcgatccaga agctgttcat gtttgataaa aaaatcaacg atctcagcta
     8221 aaaacaaata gataaaaata acctctcagc aatttccaaa tgtgagcctt ttgctcgtat
     8281 ttgaaaattg ctgtttttta ttgacacgga aagagaacgt gtgttctaat tgtgttgaag
     8341 aggtgattgt tatgtgggcg aatcaaaatc cggtatttgt ttatgaaaaa gaaccttcaa
     8401 gagatatttt atgtattgac tgcaaatctt tttacgcctc cgtagagtgc gttgaaaagg
     8461 atcttgatcc ccttacaact aaattagtag tcatgtccta tccgtccgat tcgacagaaa
     8521 cacggggtag cggattaatc ttggcttcca gtcctgcagc aaaaaaagct tacggcatca
     8581 ccaatatcag ccgtgccaga gatcttccct tcccctatcc aagtgattta tatattgttg
     8641 ccccaaggat ggcctattat atggaaaaga acagagaaat caattctatc tataaaaaat
     8701 atgcagacga agcgaaccat catgtctatt ccgtcgatga aagcttcctt gatgtaacaa
     8761 atagcatggc actctttggt aaaacaaatg cgtacgacat ggctaaactg atccataaag
     8821 atgtgtttga gcagaccgga atctacacga ctataggaat tggcgataac ccgctacttg
     8881 ctaaactagc cttagacaat ggctcaaaag acggaaagca aatgtttgct gaatggcgtt
     8941 atgaagatgt accagaaaag ttatggaaaa tctcagatat cactgacttt tggggaatcg
     9001 gcaaacgaac agcaaaacgt ctgaaacaaa tgggtataca ttctgtttat gatttagctc
     9061 acgcggatta ttatcacttg aaaagcaagc taggggtcat cggtactcag ctttatgccc
     9121 atgcttgggg catcgatcga agttttttgg gacaagaata tacaccaaaa tcaaaaagta
     9181 tcggaaatag tcaaatccta gatagagatt atacaagacg agaagaaatc gagatcgtta
     9241 tcaaagaaat ggccgaccaa gtcggcactc gcttgagacg atctgcagca aaaacacaag
     9301 tagtcagctt atgggtagga tactctttag gctatcttga tccaaatggc aagaccgggt
     9361 ttcaccagca aatgaaaatc gaagcgacaa atgctagcag agatctagca gatgctttat
     9421 tgaccatttt ccaccagcat taccactacc aggatattcg gcgtgtaggc gtgaactgtt
     9481 ccgacctcgt atacgcgaca ggtttacagc ttaacctttt tgaagatcct gaaaagcaga
     9541 taaatgaagc gaaagttgat tttgttgtcg atacgattag aaaaaaatac ggctttcaat
     9601 caatcatcca tgcgcatagt ctcttgcaag gaggacgcgc catcgcacgt agttctctcg
     9661 ttggcgggca tgcaggtggt atgagtggat tagaaggtgg aaaagatgag acggacagca
     9721 aaaaaattca ttgactataa cgagtatcga gatcggccat ttggattaaa atgggggact
     9781 gcttttgcga tggaagaact agtcaacggt atcaaaaaaa atgaagaaga agcacgcaaa
     9841 cagcctcttc ctaaacaaca aatgacattg caagaaatcg atagtatact gacagaagcc
     9901 tttttatctc gtaaagcagt aatcattcag ctcaatttaa aagatgaatt tggaagactc
     9961 ttggatgagg tcgaagggca cttcattggt gaagcctatg aagattactt tatgattgac
    10021 catcagccga tattgtggga agacgtacgg tatattgagt taaaggaaac aaaaaaatgg
    10081 tttgtgacat gagaggttgt gacagaagtg atcaacttca aaaaataaga aaggattttt
    10141 cgaaaattgc ttcttaaatt tttgtggaat tttggcttat ttttcgaaga agcaacttct
    10201 ggaacaacgt atattagata tcagaggttg tgaaaaagtc gcttagtttt gagtaacaag
    10261 ccaaggcgct gatttcacaa cctctgtcta tctatccatc tattcggtaa gattcatgca
    10321 tgacgtcttc tttatttagt atgtagagcc ctttttcttc tgaccattca acataaatca
    10381 gctgttttac atctgcatag ccttctttct tcaagttctc cataagccat gcttcagtca
    10441 attgattgga ctgaagcact ttatgctgaa tttgcccttc atctacaagc aggatcgata
    10501 aagctttgtc ttcatctgat tttttcaata cactcatttg accggcattc tctaggataa
    10561 catgtttggc attttcaaga aaaaagcatt cttgctgtct taagattgct cgcaactgct
    10621 ccatctcgat atggttattt gtcaactctg tcatgttgat gacgccgtct ttgattagaa
    10681 cagatggctc gcctttgacc caacgattga ccttttcatt tttttggacg atacgttcga
    10741 taccataaat cattactgcc caaagcgcaa tggcaaatag gacatgccct acatggacat
    10801 tgtcatcata ggttgattct tctaagatcc cacctaaaac aagcgtatag actaaatcaa
    10861 acggtgtgat ttctgacatg gatttcttcc caagcaaacg taccactaat aataaaccga
    10921 tcaacccgaa caccagtttc attgtaattg aaaaaatcat tttttctccc cctaactatc
    10981 tgttaattaa attatatatg ctttctcacg aacacgcatg gaatatacaa ggaagaaatg
    11041 tatcctttta ctaaaaaaag gactgtgaca
//
LOCUS       DACENJ010000080        10780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.080, whole genome shotgun sequence.
ACCESSION   DACENJ010000080 DACENJ010000000
VERSION     DACENJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.080"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(99..2225)
                     /gene="pnp"
                     /locus_tag="IW748_001505"
     CDS             complement(99..2225)
                     /gene="pnp"
                     /locus_tag="IW748_001505"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1104422.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2413..2682)
                     /gene="rpsO"
                     /locus_tag="IW748_001506"
     CDS             complement(2413..2682)
                     /gene="rpsO"
                     /locus_tag="IW748_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1104423.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            2933..3496
                     /locus_tag="IW748_001507"
     CDS             2933..3496
                     /locus_tag="IW748_001507"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1104424.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3749..4270)
                     /locus_tag="IW748_001508"
     CDS             complement(3749..4270)
                     /locus_tag="IW748_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1104425.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4273..5115)
                     /locus_tag="IW748_001509"
     CDS             complement(4273..5115)
                     /locus_tag="IW748_001509"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1104426.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5108..5833)
                     /locus_tag="IW748_001510"
     CDS             complement(5108..5833)
                     /locus_tag="IW748_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1104427.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6009..6305
                     /locus_tag="IW748_001511"
     CDS             6009..6305
                     /locus_tag="IW748_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104428.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            6873..8465
                     /locus_tag="IW748_001512"
     CDS             6873..8465
                     /locus_tag="IW748_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1104429.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8478..9218
                     /locus_tag="IW748_001513"
     CDS             8478..9218
                     /locus_tag="IW748_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104430.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            9386..10735
                     /locus_tag="IW748_001514"
     CDS             9386..10735
                     /locus_tag="IW748_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1104431.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
BASE COUNT         2979 a         2162 c         2153 g         3486 t
ORIGIN      
        1 gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt ccccctgtca
       61 ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc atttttcgtt
      121 gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg tttatcgatt
      181 tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac tttattgaca
      241 cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat caagttgacg
      301 aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc aactttgact
      361 tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc atcagctgaa
      421 gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc atcgatgatt
      481 ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg ttcgatctgg
      541 atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc aatcgttgaa
      601 gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc ttcagttagg
      661 atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat accatctttt
      721 gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg gatatcagtt
      781 aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc gactggcgct
      841 ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact tgcttgggaa
      901 gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg aaattcttct
      961 tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc gatttcccga
     1021 cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa attgtagtgg
     1081 tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg ctctcctaga
     1141 ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa acctgatcca
     1201 tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc caatttacgc
     1261 ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt ttcaagatct
     1321 tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc tgcaaatttc
     1381 tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc gcgagcaagt
     1441 ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa gatttctttt
     1501 ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc tactgcttga
     1561 acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa cagcaatgca
     1621 ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt gatggcttgt
     1681 ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac tgttggattc
     1741 aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc gtcaaatggg
     1801 atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc tggcgagcag
     1861 tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa gccttctgca
     1921 aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc cgtacttgga
     1981 cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat tttttcttca
     2041 taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc cacagctgca
     2101 cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg ttttgctagc
     2161 tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac ttgtttttct
     2221 gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa atgaaaactg
     2281 ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct agcttgatca
     2341 cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga atcccactct
     2401 ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt aacgttgaac
     2461 gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt tcattaggcc
     2521 gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt ggttgatttc
     2581 ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat ggcgagcgta
     2641 ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca cctctttcga
     2701 ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg caggtcatgt
     2761 gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt agttaatcat
     2821 taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt ggtaaactat
     2881 aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt ccatgattac
     2941 aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa aagaagtttc
     3001 tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt tcttaaaaaa
     3061 cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg gcttagctgc
     3121 tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc ctgacccaga
     3181 ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga gccattctgt
     3241 tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag aagtaccagg
     3301 atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg gtgaaaaaca
     3361 caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg accatatcaa
     3421 cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa aagaaggcgt
     3481 attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa gaaagcttta
     3541 taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc actaatcgaa
     3601 atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg atacgcatct
     3661 tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga tagaatagtt
     3721 tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct gcttcagcta
     3781 gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta tgcagatgga
     3841 tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt ctcataaagg
     3901 aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg taaaggggat
     3961 gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc agttcttctt
     4021 ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta ttggtttctt
     4081 ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca gctcgtagta
     4141 aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca aacttgctag
     4201 cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc cgtgtctctc
     4261 cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt gtttcatcaa
     4321 atcaacatac gtgtctgtta ttgtttcctt atatccctta tttttcagca gactaacaac
     4381 tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt cttctttttt
     4441 cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa ccaactctcg
     4501 atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta gttcttcctc
     4561 tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg gaaaagattt
     4621 ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac ccggcgtgac
     4681 gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg cgtactccag
     4741 accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct ggacattcgg
     4801 agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg tacaattgaa
     4861 atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat tgataactgg
     4921 ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt gggatcgcag
     4981 ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca tacggtcggc
     5041 aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt ctttttcggt
     5101 tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca accgctccgt
     5161 cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg attactaaga
     5221 ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact tgaaaactcg
     5281 ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc tccatgactc
     5341 catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct ccgcatgctt
     5401 gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat tttcgacaat
     5461 gacattgata cagactccca tgaaaattga cgagttgcgg gcttttagcc tttgcgtgca
     5521 aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga tgtgctattt
     5581 ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca aattgataga
     5641 atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc tgctccatcc
     5701 cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg gatacccctg
     5761 caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct tcgtctactg
     5821 ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg aacaaattta
     5881 ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga gcaatttatc
     5941 acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata aaagtgaggt
     6001 gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt atcgcctggt
     6061 tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat ggaaatcttt
     6121 gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca ttagccgtat
     6181 atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta atattagcca
     6241 tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag aaagcggcag
     6301 cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc tttttttatt
     6361 gtattacata catttttgtg tataattata ctatcgtttg caatggatag atgattcaca
     6421 ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt ttttcaaaaa
     6481 ccagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg tataaataac
     6541 tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa ccctgtggag
     6601 tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac ttttttgctt
     6661 ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt gatgctaagt
     6721 tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct ttttatatct
     6781 ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat ctagaccgct
     6841 cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt tcagttgccg
     6901 tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat gcagatgaaa
     6961 aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc ttcaactggt
     7021 ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt tacgccggcg
     7081 gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa ttagtcatcg
     7141 tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc gacgcgatca
     7201 tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc gatccttatt
     7261 acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa gcaaaaagtt
     7321 tagcagatct ttctggagca aagatcactg ctcagttaaa tacattccac tatactgtga
     7381 tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca gcaatgagaa
     7441 cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa ggtgtaacgg
     7501 caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat ggcttccaga
     7561 caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg gatatccaaa
     7621 aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt atggaccaag
     7681 cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc atttctgatt
     7741 tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga cttaccttgt
     7801 tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt ggtgtcttcc
     7861 gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc ggaaatgcac
     7921 ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa gcaatggtca
     7981 tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt gccgctttgt
     8041 ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg gggatttttg
     8101 cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat ggccaaacga
     8161 tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca ggaaatgagt
     8221 ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct gatttgttct
     8281 tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt acgatcgtcg
     8341 ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg gtagaaaaga
     8401 aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca aatttgcagg
     8461 aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta agaaaaagct
     8521 tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt gaagttgtga
     8581 cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc aatttactgg
     8641 aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct cctaaatata
     8701 atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac ctgttcaaca
     8761 atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa cgagataaag
     8821 aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat cgttatatcg
     8881 aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt gctcgggcat
     8941 tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta gaccctgaaa
     9001 tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg acaatgctta
     9061 tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt tttatggata
     9121 aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg aaagaagaac
     9181 gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg attgggacaa
     9241 agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg taaatttctg
     9301 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
     9361 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
     9421 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
     9481 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
     9541 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
     9601 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
     9661 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
     9721 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
     9781 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
     9841 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
     9901 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
     9961 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
    10021 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
    10081 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
    10141 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
    10201 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
    10261 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
    10321 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
    10381 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
    10441 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
    10501 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
    10561 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
    10621 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
    10681 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
    10741 tagttgtgac agaagtggtc agcttcgaga aataagaagg
//
LOCUS       DACENJ010000081        10751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.081, whole genome shotgun sequence.
ACCESSION   DACENJ010000081 DACENJ010000000
VERSION     DACENJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.081"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1468)
                     /locus_tag="IW748_001515"
     CDS             complement(<1..1468)
                     /locus_tag="IW748_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ1104432.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGDGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKKEGITVKDDGQTFEYTATN"
     gene            complement(1738..2985)
                     /locus_tag="IW748_001516"
     CDS             complement(1738..2985)
                     /locus_tag="IW748_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104433.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            3377..3793
                     /locus_tag="IW748_001517"
     CDS             3377..3793
                     /locus_tag="IW748_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104434.1"
                     /translation="MNKKRKLSVLITLISILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSSEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            complement(3810..4547)
                     /locus_tag="IW748_001518"
     CDS             complement(3810..4547)
                     /locus_tag="IW748_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104435.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(4969..5532)
                     /locus_tag="IW748_001519"
     CDS             complement(4969..5532)
                     /locus_tag="IW748_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104436.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(5519..6046)
                     /locus_tag="IW748_001520"
     CDS             complement(5519..6046)
                     /locus_tag="IW748_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104437.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(6069..7406)
                     /locus_tag="IW748_001521"
     CDS             complement(6069..7406)
                     /locus_tag="IW748_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1104438.1"
                     /translation="MLIVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            7526..8005
                     /locus_tag="IW748_001522"
     CDS             7526..8005
                     /locus_tag="IW748_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104439.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(8042..8274)
                     /locus_tag="IW748_001523"
                     /pseudo
     CDS             complement(8042..8274)
                     /locus_tag="IW748_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8277..8594)
                     /locus_tag="IW748_001524"
     CDS             complement(8277..8594)
                     /locus_tag="IW748_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104440.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(8591..9439)
                     /locus_tag="IW748_001525"
     CDS             complement(8591..9439)
                     /locus_tag="IW748_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104441.1"
                     /translation="MFSQEEIISKSQPDKNGTITMKKKIPCYRCSGSGQYKFMSMGKI
                     TYGTCFKCDGDGFLVVTEKSFTPEYRAKLNALNKKRADRKIRITREKESQKNFDRYGF
                     EKGYIYVVIEENSYAIKDELHHQGATYDPLLGWFFKEKQEQYKTQKVFVEEFSVINDE
                     GIIELKLEEYSERQQEILRAKRREEGEFIGTIAQRLELDLKLTNTVTIDTQWGNVYLH
                     LFEDKNHNSLIWKTNKFLYYDEWDVGEVRKVKATIKAHDYYRDTKQTWVKNVKIQKNE
                     SEGIKV"
     gene            complement(9492..9821)
                     /locus_tag="IW748_001526"
     CDS             complement(9492..9821)
                     /locus_tag="IW748_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104442.1"
                     /translation="MKLEGSLQNRMMEGRTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELIKTKNGWKVLKEDTHFYIGDRE
                     KYYDPSF"
     gene            complement(9949..10188)
                     /locus_tag="IW748_001527"
     CDS             complement(9949..10188)
                     /locus_tag="IW748_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104443.1"
                     /translation="MKELEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHFPKVRFYN"
     gene            complement(10279..10566)
                     /locus_tag="IW748_001528"
     CDS             complement(10279..10566)
                     /locus_tag="IW748_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104444.1"
                     /translation="MAEVKFGSKEWFEELNSMIQFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
BASE COUNT         3128 a         2188 c         1484 g         3951 t
ORIGIN      
        1 tgtttgtagc tgtatattca aaagtttgtc catcatcttt aacggtaatt ccttctttct
       61 tgaatacgcc tgtataacca gttggtactc cggtctcttc aaggctgtag gtcttgtttg
      121 ctacaatatc gaagcttgca agtccgtttt catctgtttt ttctgcttgt gtttgggatg
      181 aatcatccgt caaggtaaat tcagcattag ctaatggttt tccatcttgg tcaacctttt
      241 taacttttac tttacctttt ttaattgtgt tcgtagcagt gtaactaaat tcttgtccat
      301 cggtattaac agtaatacca tcctttttaa atgaaccttg ataaccttca ggaacttttg
      361 tttcttctaa gctgtatgtt aagccttgtt gcaaatcaaa cgtagctaac ccatcatctc
      421 cggttgtctt aacagattta gcattgttat tgtcagttag agtaaactcc gctcctttta
      481 aagcatttcc atcttgatca actttcttaa ctgttacctg accaacagct cttgtccatt
      541 tagcactcaa agatttagag tcctttgttt ctgaagtagt gatcataact acggcatctt
      601 gatacacacc tcgtccatca aaagtaaaag gattggttgt gctaacatta gcagtacctt
      661 taaatgacaa agtaccactt ggtgtagtca caggtacttt aatagtaaaa cttgaattta
      721 atttcacagt gttgttagta atttctttac catctgcatc ataaatcttc ataccagctg
      781 gttttgctga tgtaaaagtg agcttcgcat taccagtctt atttgatgct gttttatatg
      841 tgaaagtgtg atattttcct tcgtcagctg tcttagaagc tgtgatagaa aactcagtgt
      901 tgggcgtatc ttttccattc ttagcatagt cataaatttt ttcaaatgct ttttttacac
      961 gagctgtttc tgcggcggta tggtttggat gtgaccatac aatctgtgaa actttaaagt
     1021 ttccggctaa aacccatgaa actaattgtg ttgcatacca tgcttctgta tcatttcctg
     1081 cacccagttc ctttgctgtt tttgatggaa aaccaccttt aaacatacga tagaactcat
     1141 cactgccatc tcccttgtac ttcaagcttc cggctccact tcctgttggg gatggcaatt
     1201 ctgaatccat acaatatcca atatttccat gagaatcttt aaattgactt gaaatcatcc
     1261 attcatttgg atgattcggc atattaaccc taatataacc gtctgttttt acgccagttt
     1321 ctgtaaattt tgcacttgtc ggttttgtga catctgcttc agctcttatt gctgaaaaag
     1381 gcattataag cgtagataac accattacta aagtcgcaaa acaataaatt atttttgatt
     1441 ttaaaattat cgttttttca tgcttcatct ctcgttactc cttctttatg caagttaggc
     1501 gaagaaaatg agccacgata tgtcggtgtg gtaatgacct tcgcgcccat tcctaagtta
     1561 aaatcaccac acctattaat cacttttttc gttgaaagca agatgtttca acgctctatt
     1621 attcatagca tcaccccttt tagttctacc caccatgagc aacaagtttt ctaagtagtt
     1681 aattaaggac aaataaagac tatacttaac tacttagtat ataaacgatc tcttttatta
     1741 acgtaccgaa accgctaatt gagatacgtt atatgctgtt tcaaatgatc ctgcattaat
     1801 ccaacttgcc ccaaacacag gatcgacaac atggtaaaaa ccgtctttaa atccatcaac
     1861 ggctactaca tgtcgattcc aaatagcatc gccccaaaac attgatttat gttgagcagg
     1921 ttcaaaggca taagttaccc atgctactac aggatttcca tttacaatct catcttttaa
     1981 tccttgtacc gatgaaccag aaatatctac aacatttccg ccattagcaa caccccatga
     2041 aactacagga gccggattca taccttgata agttccatcc acattgtgtc tccattcacc
     2101 agaaaaacca ttatttggat tattatcagg agatagtggc attgatttaa caaaagtcat
     2161 taaatcttga tttgttgcaa cacctttata atgtaacgct tctagcaaag atgttccttc
     2221 gcaaagcatt gtgttacctt gactaataaa tggaacgcct agcacaacat tagttggttg
     2281 ttcgggttta cttggtgaag tagaactgtc agtttcatca ctcgaacctg tttgtgattg
     2341 atcactagaa gatgtactgg ttgattcatt tgcatcattt gatgttgatt gtgagttatt
     2401 tgttgatgtt ccagtatcag cttccgtagt gctactttca cttgttccct gtgatgcttc
     2461 tttctcactt gtagaattat ttgtattaga tgaacttgct ccatcaacac ttgaagcact
     2521 tgtttctgtc ttatcagaag tgtttgctgt tggttccgat tcttttgtag tctcggattt
     2581 tttgttctct tgctccgtat tttcaacagg atcaggttta tctttttgaa cagtttgact
     2641 aactacttcg ccatctgctt ccttaactgt taatgtatct ttactgatgt tgaaagttcg
     2701 tccaatcggg atagttcgtt gttctccttt tgccaaatca acattgttca attcggcaaa
     2761 ctcagcaaca gtagtcttgt aacctaagct tttaagcttc tctacagttg accaaatagt
     2821 atctcctttt ttgaacgcat aacttgattg tcctgttgta atttggattt gatccggtgt
     2881 gtttgcagtc cattcatcag catgtgaggt tactggtact gataaccccg caagtacagc
     2941 tgatgcaaat aaaaccatac caagcgaatt tttcttcata ttcattcctt ctttttgttt
     3001 ttattttctg tttgtgatcg caatgcttct attccttctt ccgttataat ataggttttg
     3061 tcacccactt tcttgaacga acctttaggc aaacgatttg gataatagtt gattatgttt
     3121 gtaacataat ttctaccttt acccaaaatc aaacttgctt cccttgcggt atacaagttg
     3181 tatttgtcta ggttcaattt ctttttgttt gcgatttaga cctacccctt tcgcaaaatt
     3241 tattgtataa taatagtacc attgcactgt cataattgca atattttttt ggggtttatc
     3301 ttcatttaat agcctaaaaa tgcttgttag ataaggaata tctaactaat caaaacaaaa
     3361 aaggaagtta agaaaaatga acaaaaaaag gaaattatca gtattaatta cacttatttc
     3421 tatattgact attcttagcg gtatcttgtc gctgaaaata ttcaatcaaa taaacacaag
     3481 ctcaaatgcc aaagatggaa tagtcgtaga aaatacctct aattctaaag aaactggtac
     3541 tactacaaaa aaagacaaat cttctgagat cactacgaaa aattatttaa taaagactgt
     3601 ttattttgat gaacgaaaaa cagcaattct caaagatgag gaaggatcag aattaaagct
     3661 acctataacg gtatccgata agttcaatgg aacgttctcc ttacaaaccg ctgaaggatc
     3721 acctgacaat ctcaaagttg ggcaaacaat cattcttggt aaaaacgacg aactgtattc
     3781 atttgtaaaa taaaaaaatg gagcaaaggc tagtcaagcc cttgctccat tttccattct
     3841 tcaagaggtg tagctatttc aacaccaaag tttaaatact gtggatgctg tagatacatg
     3901 atatccattc tattttcgat aaactctttt aacgttgagt agatgtattt ttctaatgct
     3961 atatctgccc aactaccata atcaagttta ttcgtaatct tgacgtttat ttcaatttga
     4021 gaaacatcaa tattttgaac atcaggaaac ttattcagtc ttagctcttc tctaaccgct
     4081 tctactagcg gttcatattg tccgcctagt tcgctaattg ttgaaactat ttcatctgaa
     4141 ttccaacgat cttccaatac ctttgctaca ttttctttag gcggttgtct ttctataaat
     4201 tcttcaggtg tttcatcaaa ctctactcta aagttcaatt tattttgatt gttagcatac
     4261 atataatcat ctctgtttga ttcaaaaaag ttacgaattc ctgtgtctga tttatcactt
     4321 aactccttat ttgctaaagt agcccaaaaa tcccaagttc ctttattttc agtatacaaa
     4381 gtaaaattaa tgtttatata gtttgcagtt tcaggatacc cttgctctct aagagcatct
     4441 ttaacaatca ctgctaagtc agtttcaaaa ttctccataa aaaattcact aaattctttt
     4501 ggattccacg tttcttctaa taacttttct aatttatgat ctgccattat tattctcccc
     4561 cactttactt tcaatcttat atctaaaact gcttaaaatt aagagctata ccttatcata
     4621 aaaggtattt tatgctttat ttaagtctac aattctatat tacaattatc atctagacca
     4681 ttttcttatg tcaaataaac catatatatt gtctttaata gttttatttg actttttaag
     4741 aaagtattta cttttgactt tttttacgtt ttacaacata tacgtattgt aatgttcagc
     4801 tcaatttatt ataatctaaa tgaattgatt aataaaaatt ctgaggttcg ggttttttat
     4861 caattctttt ctgtttgtgt ttgtgatgaa acaatccttt ttcggtaggt tttgttatca
     4921 tcgtcagcac aaagagctaa cgttggtacc gttagctctt tttttatttt actctgctct
     4981 tacatagcta tctaaatcct ccaaataaaa caattgattt tctcctatgc ttgttactcc
     5041 acgccaaatt gttgttgttg ctgttgttgg agtatttctt gtctgtgtaa tcaattcaac
     5101 cattttacct ttttgatttg ttgtatagta atcagcagta tagctagata ttggactatt
     5161 accagtgcct accatcgtgc cagaaatatc aacaggacta gactttacta tctgaggaaa
     5221 ttctttttgt atttccttca tattcttcgt atatttttcc catgaattcc agttgtaaaa
     5281 taaattaaag aatttcctac tttgttcctt aatttcagca tttcccaatt ctttgtattg
     5341 agcttcctgc atagctttca ctttctggtt attcttgact acttcgattt gtttactttt
     5401 aaggtcttcc aattctttga cattattatc ataagcattt ttcttttgaa atagtaccac
     5461 acaacaaagg atcacaatta tggaaagtat tccgatcgcc tttttagcag tagtgatttc
     5521 atgattttgc ataaaaagca cccccagtca ctttcttatc attatttagt gtgatggtta
     5581 atgtggtatc atacactttt tttccattgt attgaacttc aaaatcggtc aatgcttcat
     5641 acaacccact actagatgtc ttaattccaa agtttgttag cttaaaagta aaaggcttat
     5701 agtactcgat tttttctttc tcactactct tatcagtaat tggtggttta cctgttacaa
     5761 taaatagttt tctcaaaaat tcttcacctg attgatctgc aaacttattt tgaactgatc
     5821 cattcaacat ttcttgaata tcattttcta aatctttccg taatccatcc acctcatttg
     5881 gtttaacctc tttaaagaat gttagcttat cttcaatatg cacactgtgt ttttcagctt
     5941 ctatgtcctg attctgctta ttgaagaata ggattccaac aagcagtgct aataacatac
     6001 cagaaagcaa agaaatgata attgtatctt tcactttact attcatctat atcgtccttc
     6061 attccaattt atttttcgat ttcaaattta gcttttccta caccgctaaa gaaatgcaca
     6121 ttatctgatc cgtccactcc atcaatcaac gttaccgaaa ctccccttgt tggagcttgg
     6181 gttggtggaa cattaaagtt agcacataga aatgatccat cttcaaacac cgctataact
     6241 acaccagtat gccctgcatc tccaacagcc cataaccatc catccgaagc actaaaccca
     6301 taacctacga ttggtttgtt cgttattttt gctcctgccg atttgtagct ttcccataca
     6361 tagccaccat ttccagtagt cggttgattc ccatcgtata attggctcat ataagcccac
     6421 gtcaactctg tacactgacc ttgatatatt ccgttaaaag gtgaagttga ccaatctggt
     6481 tctttgaatt ttggtagcgt gatcgctttc ccatattttt tcaaagcttc ttcataagtc
     6541 atattcgcta ttacttgacc ttttttaccg ttaatttttc cttctttccc aattgatcca
     6601 gaaccagaat tagcgtcatc cgtagttgca caggcatctt ctactgttcc tgatgctgaa
     6661 cttgatgaat tattcgatcc atctccggta cttgaacctc cggatatttt attgatctta
     6721 ctttcatctt ttgcaccgtc catacccttg cttttcatgt acttccagat ttcttttgca
     6781 ttttcaccac gtacagctat tacagctgat tcggaatcag ctgaaatttc ccagttttta
     6841 tgaaaagtta ctgcattttc tacgacatca tcagaagcat ttaaagcata atcttttaat
     6901 attcctacga atcccccatc acctttgaac ataaagtcca attgggcttc tgtagtccac
     6961 caagaaccgc cattattttt cttagaccaa tcaactaatg ctgtatgtcg cccagctgtc
     7021 cattgaccat agccaatacc catcagacca tcagtaaatt gtttgatgaa gtcaacttct
     7081 ctctgtccat ctccgtaata cccttctact gctcgaggat caatacctga ttcttgtttc
     7141 cagttaccta aaatacctgc aatactagca cctgataagc catattctcc attcatgtat
     7201 ttgtaaatat cttcaatatt tttggctaca tcacctgtta cagcgccact tccagtatcg
     7261 tttccatcgc tatctgttcc tgacccacta ccttcagtag tacagttaaa ttgtgcatca
     7321 taagtagcta tcccatcagc atgtatcact tgcggattta ttgtgattac actcattgca
     7381 attcctgtaa attgcacaat taacataagc aacgtggcaa tctttctctt agattttttc
     7441 aataactacg cccccaaaaa gtgtactttt ttatgttatt atgataatat aataacataa
     7501 attaatagaa aggaaaagct aataaatgac tgataaaaca agtgatttac ctaaactagc
     7561 tccaaaaaca tctcgagtaa agagcaaaaa agatggaaaa acaaaaaatg ctgtaaagaa
     7621 aaagtcagta aaagaaacat cctcgttact acttgatatt ccaatgagtt cttttgatga
     7681 caatacggac gaaacttctg ttaaaattgc aactcacgtt ggaatgaaca atcaaaatga
     7741 ttctttttac aacttgttaa acagcctaag agagttggaa gataaaaaaa atcaggtaat
     7801 cgcatcaaaa gctcctgatc tcacaaccga tttggatgct gaaattatcc aaatcaaaca
     7861 agatgagcga tcagttaaat cgctactgaa aacctacttt gatggttttg ataaagaact
     7921 agctccttat agccaagcaa tcctaactaa accaatacaa gctccacata tttcaatttc
     7981 cctacaaaaa aagagtgatg attaaacaat cattactctt ttttccatta tagatttccc
     8041 tctattccat taaatcgaaa gtcacaccga ttactacaaa gattaacgtg accacattca
     8101 aaaacattaa aagatatgct gataatctca tctttttttt agttcactta ctggtaatct
     8161 aaacctttga acgattgcac tggaaatacc ataattagca atgacaaaca aaaaatacgc
     8221 aataatttca cttacaccta gatagatcag taaaataaat aagtcactta acattgttag
     8281 tttcctccca gtatatgatt ttcatttttg ggaagtcttt tataatccaa ttcctcaaaa
     8341 aaaggaacca tagtgtcact aatataggtt ttagtttcag gtaccgccaa aattccccaa
     8401 tcaagaattt cttcccatga tacatcatca aattctaaaa tattatgatc aactgaccta
     8461 ttaccaagtt catatccacc aaagatcacc atattatcaa tcatgtatag aatttcatca
     8521 ggcgaaccat gagttaacgt ttcgtcctct tcaagcagtt cgtttaatct ttgtttgtat
     8581 tcctctcttt tcatactttt atcccttctg actcattttt ttgaatttta acatttttaa
     8641 cccaagtctg cttggtatct ctgtagtagt catgcgcttt tattgtcgct ttaactttcc
     8701 ttacttctcc aacatcccat tcatcataat aaagaaattt atttgtcttc caaatcaatg
     8761 aattatggtt tttatcttca aataaatgca gataaacatt gccccattgt gtatctatgg
     8821 tgacggtatt ggtaagcttc aaatccaatt ccaatcgttg agcgattgta ccaataaact
     8881 caccctcttc tctacgcttc gcccttaaaa tctcctgttg tctttcgctg tattcttcta
     8941 gcttcaattc aataattcct tcatcattga ttacggaaaa ttcttctaca aaaacttttt
     9001 gagttttgta ttgttcttgt ttttctttga aaaaccaacc taataaagga tcataagtag
     9061 ctccttgatg atgcagttca tctttgatcg catagctatt ttcctctata accacgtaaa
     9121 tataaccctt ttcaaaacca taacgatcaa aatttttctg agattctttc tctcttgtga
     9181 tccttatttt tcgatcagcc ctttttttat ttaaagcatt tagcttagct ctatattctg
     9241 gcgtaaatga tttttcagtt acaacaagaa atccgtcacc atcgcactta aagcatgtgc
     9301 cataggtaat tttccccata gacataaact tgtactgacc tgatccgcta caacgatagc
     9361 aaggaatttt tttcttcatg gttatagttc catttttatc cggttgcgat ttgctgatga
     9421 tttcttcttg actgaacatc cttcatactc ctttctgagc gaggataaaa cctcgctcat
     9481 cttcagtttg tttaaaaact aggatcgtag tatttttctc tatcacctat ataaaaatga
     9541 gtatcttctt taagcacttt ccatccattt tttgtcttaa ttaattccaa tatttgacct
     9601 tcaggatttc tgctgtattc ccactcttga tctggaaaag tagctttatt gattgcccca
     9661 tccgcttgaa ccaaaattct gtttttgctt tttacttcaa tcactgtata aggatttctg
     9721 tcggtatata gaagctcggt tgctcccata ccaactacaa tctccttatt tgttgttctg
     9781 ccttccatca ttctgttttg taagctacct tctaatttca tttcagatca tcctttctta
     9841 acttatctac aaatatatta tacacaccga tacacatttt gtaaataaat aaatttaaca
     9901 aatagataaa aaacgatgcc tatttagcat cgtttcattt gttaatgttt aattataaaa
     9961 tcggacttta ggaaaatgat ctggaaaaat agactctgtt tctatttctt gaatgagaga
    10021 aactctctct tccatcaatt gtttatactt tgatgattct ataatttcac ttctagattt
    10081 atttaaacac ctcatggatt catagtaact aaacatccta tttctatttt catttaacag
    10141 ttccaatctt tcctgatttc ttttcttaat accttctagt tctttcaaat aatttatccc
    10201 ctttcatacc aattatgatg ccatattgga tgaaataatc acatactatt tttgaccaaa
    10261 taactggtca atttagaatt atttacacca gatatccttc agatttttta ctacttcata
    10321 gtattcttgt gagtcttgaa cctcttttac cgccacaacc gctgtcaagt ctttaggatg
    10381 aaatctgagt gctgatggtt ttatcatact ccatcgagta ttttctaatc ttcccttagt
    10441 ttcaagtaaa gctacttgaa tgatattgtc tcttttgttg tagtaaaaag tgaacttttc
    10501 tggaaactga atcattgagt ttaactcttc aaaccattct tttgatccaa atttaacttc
    10561 tgccattttc ctatctcctt taatttcatt tttcgtctat tgttaattaa ttgcattgcg
    10621 ttcctttata ctagctagat aatcgtttat tttagctata tttttgtctg cataccattt
    10681 tggataaagc atgtaagcat gactatcttt acatgctatt aatttcaata ctttattaat
    10741 tcgtccttct g
//
LOCUS       DACENJ010000082        10711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.082, whole genome shotgun sequence.
ACCESSION   DACENJ010000082 DACENJ010000000
VERSION     DACENJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.082"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(69..2144)
                     /locus_tag="IW748_001529"
     CDS             complement(69..2144)
                     /locus_tag="IW748_001529"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1104445.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2146..3063)
                     /gene="glyQ"
                     /locus_tag="IW748_001530"
     CDS             complement(2146..3063)
                     /gene="glyQ"
                     /locus_tag="IW748_001530"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1104446.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3447..4259)
                     /gene="recO"
                     /locus_tag="IW748_001531"
     CDS             complement(3447..4259)
                     /gene="recO"
                     /locus_tag="IW748_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1104447.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4402..5301)
                     /locus_tag="IW748_001532"
     CDS             complement(4402..5301)
                     /locus_tag="IW748_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1104448.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5316..5717)
                     /locus_tag="IW748_001533"
     CDS             complement(5316..5717)
                     /locus_tag="IW748_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1104449.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5695..6171)
                     /gene="ybeY"
                     /locus_tag="IW748_001534"
     CDS             complement(5695..6171)
                     /gene="ybeY"
                     /locus_tag="IW748_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1104450.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6188..8380)
                     /locus_tag="IW748_001535"
     CDS             complement(6188..8380)
                     /locus_tag="IW748_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1104451.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8404..9375)
                     /locus_tag="IW748_001536"
     CDS             complement(8404..9375)
                     /locus_tag="IW748_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1104452.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10080..>10711
                     /locus_tag="IW748_001537"
     CDS             10080..>10711
                     /locus_tag="IW748_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1104453.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLA"
BASE COUNT         2896 a         2270 c         1943 g         3602 t
ORIGIN      
        1 ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt ttatttacat
       61 attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc catggcagca
      121 atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc catgaccatc
      181 gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc ataattttcg
      241 ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg cagttctttc
      301 tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag ctccgctttt
      361 ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc ttcatctgca
      421 agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt ttgttctgcg
      481 ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa ttggcgcaga
      541 cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat gccaaaacga
      601 tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa aggaaaggcc
      661 cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa agcatatgga
      721 tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact atctaattta
      781 tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc actggatata
      841 ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt aagcagagca
      901 tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt ggttactaga
      961 tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc agacagtcct
     1021 acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc atagatgcta
     1081 ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc gattgaagct
     1141 ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa tactttttga
     1201 ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga aataaaatga
     1261 ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc tttcatagaa
     1321 gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc agaaaagaca
     1381 gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag atcaatgatc
     1441 cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt tcttttttgc
     1501 ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc atgcgcaaat
     1561 gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt gatatccaag
     1621 atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat aggacggata
     1681 tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct tttaatgaca
     1741 tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt cgttacatag
     1801 acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag accttgtccg
     1861 cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc aatttttttg
     1921 gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt tgttactttg
     1981 attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa ttggttttca
     2041 tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac aacatgagca
     2101 ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt cagcctcctt
     2161 cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt tttcgcgttc
     2221 tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa gataacctgc
     2281 acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat ggctgcattt
     2341 caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt tagcttcttt
     2401 ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa aagaatattt
     2461 ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat cagcccattc
     2521 taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta gtccataagt
     2581 aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt acgtgaattg
     2641 tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac ccatcgatgg
     2701 gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa ttcctaaaaa
     2761 acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca ttacgacttg
     2821 aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt cagctggtcg
     2881 gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc gtaagaatgt
     2941 atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca gcatgcagcc
     3001 gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca attttttact
     3061 catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac aggaaagaaa
     3121 acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc gcggttccac
     3181 cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg cctcttcaaa
     3241 aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt ttttactgat
     3301 cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga tcctctcagt
     3361 gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt gtcaaatagt
     3421 tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc tttttcaggc
     3481 atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc aatgaacttt
     3541 ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag gtcacggatt
     3601 gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga aatagccgaa
     3661 aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc gtccattggc
     3721 cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt tccttttgtt
     3781 tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg cagtatttga
     3841 acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg ctgcagagct
     3901 tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc tgcatctact
     3961 agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat gttccgaaac
     4021 gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa attaccgata
     4081 tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg ccgatggatt
     4141 cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac taatttatct
     4201 ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag ctggctcata
     4261 ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga ttatttatgt
     4321 tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt tctataccta
     4381 acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt cttgcagata
     4441 gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa atactttgtc
     4501 tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca gcatttttcc
     4561 acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg tggcctggat
     4621 atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga cagaatgagg
     4681 cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa cgatgaagta
     4741 ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg gcatttcact
     4801 gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga tcggaatcac
     4861 ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact cgtctggatg
     4921 gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt ttagacgttc
     4981 aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa ataagactgc
     5041 atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta gacgatgctt
     5101 tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg tagtatatac
     5161 tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa ttttttgacc
     5221 cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga ttgcgacaaa
     5281 ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa atgatatatt
     5341 ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac taaaactgct
     5401 ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc cgtaaacatg
     5461 tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag aaaaacagct
     5521 aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat aaataaagca
     5581 aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac ggttttgact
     5641 ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac gtttttatct
     5701 tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt tctcatcttc
     5761 tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta aaaagccaag
     5821 ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt caatagagat
     5881 catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa tcactgggat
     5941 ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg catcttttcc
     6001 acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa cagacatttc
     6061 tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat cgatttcttt
     6121 cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca ttatacatcg
     6181 cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc attttaggat
     6241 atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt tccacttttc
     6301 ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata cgctcttcga
     6361 taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt gcccgtactg
     6421 cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc ggttttggtc
     6481 caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct ttgatgtaga
     6541 aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc atttgcggca
     6601 tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata atatgtttac
     6661 tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag ttctcaacga
     6721 aaaagctagc gtgtttgatc ttgccgatat catgataata gcaagctact cgagttagta
     6781 aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc atcatactat
     6841 gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga tttggattgc
     6901 tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata tatgggtgta
     6961 gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca agcattaacc
     7021 aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca tccatcatca
     7081 caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc cgtttacgtc
     7141 tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc gttagaaagt
     7201 tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa ataccagcca
     7261 tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa gcagcgggat
     7321 aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat ttcatcagca
     7381 ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt tctcctgtat
     7441 tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca gctaaaacca
     7501 tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat tccaattttt
     7561 tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct tggaagatca
     7621 tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt ttctcattca
     7681 atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt tgttgtgctg
     7741 gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct tccaattcac
     7801 tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca tccctcacag
     7861 tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc ggtagttttt
     7921 gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc gcggcgatcc
     7981 gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca atcttttcgt
     8041 cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt tgcttgatcc
     8101 gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg actgcttctg
     8161 ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg ttcgcacgga
     8221 tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg acactactaa
     8281 atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat gctgcctttc
     8341 caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc tcctaagcaa
     8401 agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg caactacagg
     8461 gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga tttgctttaa
     8521 tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa tctgactcgt
     8581 atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca ttttcatctg
     8641 agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca atgtccgccc
     8701 ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc ggttcgtatg
     8761 atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag gatctacttt
     8821 ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag caggccgcgt
     8881 caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata ctactgcaag
     8941 aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt tggctaccga
     9001 ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat tacggtcttt
     9061 gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt gccccatttt
     9121 cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca attcctgcaa
     9181 tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga tctgaatgat
     9241 ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta tgtgcttgtc
     9301 gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct ctaaagaact
     9361 agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc ctattttacc
     9421 atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg cttaaccata
     9481 agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg tttgaataaa
     9541 aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc taacttagca
     9601 agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt gaaatattct
     9661 tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca gcaaagcgtc
     9721 gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag tgagaaatga
     9781 caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta gatcattgtg
     9841 gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg aatgaagaag
     9901 cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt cccaagtgga
     9961 aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat tagtggtatt
    10021 tctagtttct gcaacgttta ttttgttact caaaatgata attattggag gaaaaattta
    10081 tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca cttatcttcg
    10141 ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa atggctggat
    10201 caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc ccattattag
    10261 ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct aaacctgtcg
    10321 gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga ccattctttg
    10381 gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt ttgcctgaaa
    10441 aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt gcttatttcc
    10501 ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac tccttgtttc
    10561 ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg aatctaaacc
    10621 aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta gaaggataca
    10681 acacggttga tgcagtagct ttacttgctt t
//
LOCUS       DACENJ010000083        10649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.083, whole genome shotgun sequence.
ACCESSION   DACENJ010000083 DACENJ010000000
VERSION     DACENJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.083"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            81..1340
                     /gene="lysA"
                     /locus_tag="IW748_001538"
     CDS             81..1340
                     /gene="lysA"
                     /locus_tag="IW748_001538"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1104454.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1327..2127
                     /gene="dapF"
                     /locus_tag="IW748_001539"
     CDS             1327..2127
                     /gene="dapF"
                     /locus_tag="IW748_001539"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1104455.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2130..3011
                     /locus_tag="IW748_001540"
     CDS             2130..3011
                     /locus_tag="IW748_001540"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1104456.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3008..3724
                     /locus_tag="IW748_001541"
     CDS             3008..3724
                     /locus_tag="IW748_001541"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1104457.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3730..4425
                     /gene="dapD"
                     /locus_tag="IW748_001542"
     CDS             3730..4425
                     /gene="dapD"
                     /locus_tag="IW748_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1104458.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4441..5442
                     /locus_tag="IW748_001543"
     CDS             4441..5442
                     /locus_tag="IW748_001543"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1104459.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEKPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5439..6800
                     /locus_tag="IW748_001544"
     CDS             5439..6800
                     /locus_tag="IW748_001544"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1104460.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6760..7911
                     /locus_tag="IW748_001545"
     CDS             6760..7911
                     /locus_tag="IW748_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1104461.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7886..9055
                     /locus_tag="IW748_001546"
     CDS             7886..9055
                     /locus_tag="IW748_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1104462.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9230..10609
                     /locus_tag="IW748_001547"
     CDS             9230..10609
                     /locus_tag="IW748_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1104463.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3535 a         1866 c         2396 g         2852 t
ORIGIN      
        1 tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa
       61 gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca gcaagcacat
      121 tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg agacaaacga
      181 tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg ctttacgcct
      241 ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga ttcgggctag
      301 atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct agagatacta
      361 tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt gaaaatgatt
      421 tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa ttaagccaac
      481 gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa gcacatactc
      541 atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat gaaagtaaag
      601 agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta gaaggatttc
      661 atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa atcgacaaat
      721 tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc ggagggtttg
      781 ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta aaaaaactga
      841 ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg
      901 aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt ggtttcatca
      961 aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat aatatccgtc
     1021 cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa
     1081 cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag
     1141 cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg ggagcttatg
     1201 gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt tttgtcaata
     1261 atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta aggggggaag
     1321 tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt attagattac
     1381 gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga aaaatatgat
     1441 atagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta caacaaagat
     1501 ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata cgtaaatgat
     1561 cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac aagaaaaatt
     1621 acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc agatgattct
     1681 ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat agagatcaat
     1741 ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcataccgt tgtttttgtt
     1801 gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca tcatcctttg
     1861 ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga attgatcgtc
     1921 cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg ctgtgcctct
     1981 tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt tcacttggaa
     2041 caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc agcagttcat
     2101 gaatggacaa gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca
     2161 cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg atccaattcc
     2221 atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa tcttctacgc
     2281 tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg gctggccgtg
     2341 tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa aaagcgaagc
     2401 attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac aacaaaacaa
     2461 gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg attccgctta
     2521 ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta ttagtaaatt
     2581 tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg gcttatgtga
     2641 tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat gatgatttga
     2701 tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg aatatccaac
     2761 ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag caagcaaaag
     2821 aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc aatcctattc
     2881 ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc ctgcctttag
     2941 tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa gaaaggacgg
     3001 ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag tcgaagaagt
     3061 tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag aagcaactac
     3121 gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag ataatttaga
     3181 gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca cgacaggtta
     3241 tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg tgatgtatag
     3301 tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag cagctgcaat
     3361 gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa aagatgcacc
     3421 atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt
     3481 atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc
     3541 aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa tatttacgat
     3601 caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag cagcggactg
     3661 gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg acaaaggggt
     3721 gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc taaaaagacg
     3781 cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt
     3841 ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc ggttttagaa
     3901 gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt
     3961 cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat tatcagagat
     4021 caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa tattggtgca
     4081 gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg agcgacagtg
     4141 ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga accagcatcg
     4201 gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt gattgttgaa
     4261 ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg
     4321 gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag tgatgatcag
     4381 acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag atggagaaaa
     4441 atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca aacgatgctc
     4501 aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc ttcaaaacgt
     4561 tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga attgacagaa
     4621 acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt atccaaagag
     4681 tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc agcatggcgg
     4741 gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta tacattgaat
     4801 cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct taaagcttta
     4861 caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt ttctggttcc
     4921 gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg acgagaaacc agttttgtat
     4981 ccttataata ttagccaaac ggtgattccg gaaatcgatc agcctttgga aaatgggtat
     5041 acaaaagaag aacagaaaat gataaatgag acccgtaaga ttctgcatca gcctgctttg
     5101 ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg gacatggagt ctctgtggca
     5161 gttcaactgc aaaaagagtt tacgacggaa gaaattagag agtgtctcag ccagtttcca
     5221 gggattcaat tagaagatga tctagcagca cataaatatc cgacgagcat tcttgctaga
     5281 ggaacagata tggtttatgt tgggaggatc agaaaagaca cttctttaga gaacggattg
     5341 ttgttctata cgactgccga taacatacgc aaaggagccg ctgcgaatgc cgtacagatt
     5401 gctgcagctt taatgcagaa aatagcggag gtggcaagat gagtaatacg ctgaaagtag
     5461 cgaaattcgg cggaacatcg atgtctcatg aagagcaatt caaaaaagtc cgtacaattg
     5521 ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag
     5581 atgatgacaa agtaacagac ttactttatt tgatttatgc ttaccttcag catcatgttg
     5641 attggacacc cctttggaaa aggatttgtg atcgtttcat tcaagtaaga gatgttcttg
     5701 ccctgcagtt acctattgaa gatgaactag cacgcataca gaaacaactg gaagacgaaa
     5761 agataactga agattattta gtaagtagag gtgaatatct gactgctcaa ctgatggctg
     5821 agtatcttgg ttaccagttt attgacgcaa aagacctgat tttctttgac tatgatggga
     5881 aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa
     5941 tggttgttcc agggttttac ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg
     6001 ggggaagcga cattaccggc gcgatactgg cgaagatgtt gcaagcggaa agttatgaga
     6061 attggacgga tgtttcagga atcatgatgg ctgatccgcg aatcatcgat catcctaaaa
     6121 tcatcgagga aatctctttt agagaactga gagagatggc ttatatgggg gctaacgttc
     6181 ttcatgaagc agctgttttc ccagttcaag aggctgatat ccccatccaa ataaaaaata
     6241 ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg
     6301 gactgacagg aatagctgga cgcaaggatt ttctttccat cactttattc aaacgtcata
     6361 tgtccgatga aatcgggttt atctggaaag caatgagcat ttttgctaaa catgagatat
     6421 caatcgaaca tattccttca gggatcgaca atatcggagt gatcgtctca gcagaagcga
     6481 ttgccgataa gttatttctc atcacgaaag aattaaaaga aacacttggc gtggaagaaa
     6541 tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg gggtccgcat aaagaattga
     6601 ttggattatc tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc acatcgatct
     6661 tatcacaagg agcacaggag ttgaatctga tcatcggtgt accgaataat caatatgaaa
     6721 cagtagtgaa gggtatctat gaagggatgg tgaatacgga tgctgaaaga atatcgaatg
     6781 gcactccatc aaattcctga aatcgggttt catgaattca agacaaaaaa atatttatat
     6841 gaacaagtaa aaaacagcga ggccgttatc catgaaatcg gcgaaacagg actactcctt
     6901 tattttgata atcagcaaga agaaacgatt gcttttcgaa cagatatcga tgcactgcca
     6961 gtcaaagaag aaacgaatct ctcttttgct tctaagcatg aaggttatat gcatgcatgt
     7021 ggtcatgacg gacatatggc gatgctttta gggttgactg actttataac taaacacatt
     7081 gaggatctta tttataatgt agtactgatc tatcagccat cagaagaaat ttccggtggc
     7141 gcacaaagcg ttattcagtc aggattgttg gataaatatg aagtacaagc aatatttggc
     7201 tttcatctat ggcctggact agctaaggga gaagttttct ctcgaccagg acctttgatg
     7261 gcacaaagca gtgaaacaga cattatcatt aaaggccagt ctgcacatat tgcttctagt
     7321 gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt tgaagaatgt ttatgatttt
     7381 gaccggcaat taccggcaga gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga
     7441 acgatccgaa atgttttagc tggagaagtt attgtatccg gaagtatccg ttcttacagt
     7501 tcagatatgc aaaattattt gaagagggga ttagaccatt tagcagaaca gtttcactct
     7561 acagctacta tttcttttcg ctataatgac ggctatccag ctgtaatcaa ccatagaggc
     7621 ttatatgcag cactgttaga ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa
     7681 gccgaagatt ttggcatata tacgcaacaa tatccatgtg tatttttctt tttaggtgtc
     7741 ggtgataccc ctgctttaca tgatccgacg ttcgattttg atatggatgt tttagaaaat
     7801 ggtgtagaat ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa aatcgatagc
     7861 agttcaaaag aggagcgagt aaataatgat gaatttatcc aatcgattta gtcctaggct
     7921 ttctagaata gaagtatcaa aaatacgaca attcgatcag caaatatcgt cgataccaga
     7981 tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact cctgaacatg tcaagcaagc
     8041 aggaatagca gcgattgaag aagacttttc tcactatacc ggaatgagag gattagagga
     8101 actgcgggaa gcggcttgta tatttcagca acagcgttat ggattaactt atgatccgca
     8161 aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt
     8221 tttggaagaa ggagacaagg ttcttattcc agcgcctgct tattcaggat atcagccatt
     8281 agtggaacta gctggtgctg aattgatccc gatagatact tctgacacag ggtttgtctg
     8341 tcagccggaa caatttgaac gagcatttga acaatatgga tcagctgtca aagcagtcat
     8401 tttaaactat cctaacaatc ctactgggac gacattgtct gcaaaacaat tgaaagcaat
     8461 cgctgaggtt ttaaaaaaat atccggtatt tgttataagt gatgaagtat atgctgaatt
     8521 gacttatagt ggaacgcata tgtccatagc tacttatctt ccagaacaaa caatcgtgat
     8581 aagcgggtta tccaagtcac atgcgatgac tggttggcga gtgggcttta tttttgctca
     8641 aaaagccctt attgacgaac taattaaagt ccatcaatat ctggtgactg ccgctacaac
     8701 gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg gtgaaagata gcgagaatat
     8761 gaaagagcaa tatcaaacac gaagagacta tctggtccag caattggcgc ctttaggatt
     8821 tgaagtgacc cagccaaacg gtgcatttta cttgttttgc aaactcccag ctgccattca
     8881 aactgactct tggcagtttt gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc
     8941 aggaattgca tttggcccag aaggagaagg ttatatccgg atttcttatg ctagcagcat
     9001 ggaaaaatta catgaggcct gcaaacggat caaagacttt ttaacagaac agtgattcat
     9061 agattttatc aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga gacgattgtt
     9121 ttttaaattt agttcaaatg aacgcgtttc ttttgattca taactaatgt aatgttaagt
     9181 ttaaattgtt tagaatgatt ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat
     9241 caaagttgtc attgtaggtg catctcatgg agggcatcaa tcgatcttgg aactattgac
     9301 acgatacggt gaaaatgtag atatcacgct atttgaagca ggagactata tttccttcat
     9361 gtcatgtggt atggagctgt atttagaaga caaggtaaca gatgtaaatg atgtacgtaa
     9421 tttcagacca gaaaactttc cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa
     9481 aacgatcgat gccgataaaa aaacagtaac ggttactagc gcatcagata acgaaacaaa
     9541 agaatatcct tatgacaaat tgattttgag ttctggtgtc aaaccaaaca atctgccagt
     9601 accaggagca gatctagaaa atgtctactt gatgcgtgga tacaattggg caaccaaaat
     9661 caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg cgggttacat
     9721 tgggatcgaa gcagcagaag caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt
     9781 gatcgataga cctttaggca cttatttaga tgctgagatg acagatattt tagaacaaca
     9841 cctaaatgaa aacggtattg aagttttgac aaatgcaaaa atcgaagctt ttactggtaa
     9901 cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc ccagcagatc tagtcattca
     9961 agctgccggt gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact tagatgaacg
    10021 tggctggatc caaacaaatg aatacttgca aacgaatctt ccagatgtgt atgcagtagg
    10081 agatgctaca cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac
    10141 tgttgcacgt agagaagcac gttatgtagt aaaacatctt tttgaagaag taccaaacaa
    10201 accgttcggc ggagtcgtag gttcctctgc attaagtgtc tttgactatc attttgcagc
    10261 aagcggattg aatagcttta cagcagcaaa agcaggagta gatgtacgag tgtcttatta
    10321 tgaagatacg ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa aagtgtccat
    10381 ccaattattc tcggatccaa tgacccatca attgctagga ggagcagtat tatctaccta
    10441 tgatgttaca ggtcaaggaa atgtattggc actagcgatc caaaacaaac tgactttaga
    10501 agaactagca gaagctgact tcttcttcca gccaggcttt gaccgacaat ggagcatgtt
    10561 gaatctagcc gctcaacaag cgttaggaga agaaccattt gtagaataag aaaacgaaat
    10621 atttgaaaag aggttgtgaa aaagctgcc
//
LOCUS       DACENJ010000084        10542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.084, whole genome shotgun sequence.
ACCESSION   DACENJ010000084 DACENJ010000000
VERSION     DACENJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.084"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..578)
                     /locus_tag="IW748_001548"
     CDS             complement(<1..578)
                     /locus_tag="IW748_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104464.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLN"
     gene            complement(595..1920)
                     /locus_tag="IW748_001549"
     CDS             complement(595..1920)
                     /locus_tag="IW748_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104465.1"
                     /translation="MIVAAMSYHLTGNSNVASLIAVVGVVYAIVKACNNEYLAIWVMF
                     VLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKVKARSRYGIVYSVMLIFIL
                     IACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSERITKNEFIDLVCKFTRWT
                     MCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGYNST
                     NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALIACL
                     VSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDARAV
                     EYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRDKSV
                     AAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            complement(2050..3254)
                     /locus_tag="IW748_001550"
                     /pseudo
     CDS             complement(2050..3254)
                     /locus_tag="IW748_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(3287..3940)
                     /locus_tag="IW748_001551"
     CDS             complement(3287..3940)
                     /locus_tag="IW748_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1104466.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            complement(3974..5260)
                     /locus_tag="IW748_001552"
     CDS             complement(3974..5260)
                     /locus_tag="IW748_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAQ1104467.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            complement(5283..6284)
                     /locus_tag="IW748_001553"
     CDS             complement(5283..6284)
                     /locus_tag="IW748_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ1104468.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            complement(6311..7078)
                     /locus_tag="IW748_001554"
     CDS             complement(6311..7078)
                     /locus_tag="IW748_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ1104469.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            complement(7884..9197)
                     /locus_tag="IW748_001555"
     CDS             complement(7884..9197)
                     /locus_tag="IW748_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1104470.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            complement(9226..9906)
                     /locus_tag="IW748_001556"
     CDS             complement(9226..9906)
                     /locus_tag="IW748_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1104471.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
BASE COUNT         3178 a         2450 c         1508 g         3406 t
ORIGIN      
        1 atattcaata atgtttggaa atactttagt acatcatttg catatagtct ttgatcagtt
       61 cgcgttttca gcgcataact acaccccagt ttttgcgcca tttttacgcc ttcgtatgca
      121 gaagtaagtt gtagattaat attgtacgca cctgtttttt caggtttctt gcataataca
      181 acatctgcac cagcttcacg caacttgcat tctgtctctt cagataatcc tatccaagta
      241 gacactacaa gttttacttt aggatagtat ttattataca ttttaacggt atttagtgta
      301 aaatcaccgt cttctaatac tggaccttga agaactatac atatgtcctc aaaactttcc
      361 ccagcccatt gcatttgagc catgttgtct acgtttttag gtaaagttgt atattgaatc
      421 acttttctac tgttattgga gtaggttgtt acagtaggcc acaatattcc cttctccata
      481 taatctgatt tcccactaaa atacgctaac ggcgcattta tcacttttat gattggtgaa
      541 atcatatttg ctattttctt taatcgatct aaattcatct gtgctctccc ttacctattc
      601 aatatatatg aattcatcat tgaatgccga tatttctatc aaaattaaag aaatcataaa
      661 tccgaacggt cttgctgtaa tatacgctag caaattaaaa ttaagcgcca taaacaccat
      721 gaatgccata cccgcaaatg cggcgaccga tttgtctctt cgaaatccgg catctataat
      781 acttatagct aaagcaacta tatataacgc atatgcaatt ataccaaatc tataacgaat
      841 ttcaaaagcg cctaagtcct caagataata gggattatat tttttagact caaaatctat
      901 tgttttgcct gaatattcta cagctctggc atcatgattg tttggtatac caactccaaa
      961 tagttcatga ccttctaatt tattttcaaa ataatcttgt tcagcaattc tatatgcgat
     1021 attacctgtg tcactgctct gagactctac aaacgaagat ataacgacct ctctaatttg
     1081 ggtcacagac actagacatg caattaaggc aattcctaat ataccaaatc taataatctt
     1141 ggatggttta agcgaaatta aaagcataat aaccgaggtt attactacat ataatatacc
     1201 acttctgcct tgcgagatta taacaaatgt cagtaaacta aaaatgcaat tccatatact
     1261 tttatttgta ctattatagc cctctctttt atatataacc gcatatagat ttataaagaa
     1321 atataaaatg ataaatatcg gatctatata gattctagta ccccagcgtg cacctgtctg
     1381 taaaaaggtt atattgaatc ccatattaac aagaatccat tcaaaccaat atatacacat
     1441 tgtccatctt gtgaatttac acactaaatc aatgaattca tttttagtta ttctttctga
     1501 atatactatt tttactatcg gccaaaagga tattacaact agcaattctc tagctgttaa
     1561 gagcgttgta tgtacatctt gtccatagat tgaatgcgat tgcacacatg caatcaaaat
     1621 aaatataagc attacactat agacaatccc gtatctactc ctagctttaa ctttgatgaa
     1681 tctcaagcag agaaatatcg ctactataac attaaaatct gaccaatgca acacaccact
     1741 accaatgtta atatatggca ctatccccca tatatcagaa cctaatacaa acataaccca
     1801 tatagccaga tactcgttat tacacgcctt tacaatagca taaactactc ctactacagc
     1861 aattaatgac gctacattgg aattgcctgt aaggtggtaa ctcatagccg caacaatcac
     1921 acaaacataa aataacagga gttttctaaa ataatatgat attcttattt ccaagtttct
     1981 tttcctttct ctcagccatc tccatatatt tcctcctata aatcatcatc agataagctt
     2041 atatatatat tagctgttaa ttatcgcact aaactcatct tgtctagccg ttaaggagta
     2101 tttttctttt aagaataatt ttgcattctt tcttactttt tcattcaaat tcatgtcttc
     2161 aagcagtcta actacagccg ttgcaaaatc tttttcgtta tctgctataa ataactcact
     2221 tccatctttt gcgccaatcg tatcacaccc aactaatgta gatacaactg caatttcatt
     2281 attgaacgct tctaatattt ttattcttat accactacct tctaacaaag gtacaattag
     2341 caccgcttga tcgtcataaa tgtgtgaaat atcttttaca tacccacgat aatccacgcc
     2401 tgaatattta ctaaaatctg ttccaactgg cccgctaccg acaattgtga tttttacatc
     2461 cggaattctt tgttgaatat ttggataaat tgactctaaa aaccaaacta atccttgcat
     2521 attggatttc caagaaaggt tacccaaata taggatcgtg ttcttctcta tatttttttc
     2581 acccctgtaa gcatttcata agttggaagc atcgtataac tgttgtcatt aatctttttc
     2641 agtacttccg tatttgcatc acacacacta aagcaataat cggccctatt tactgcggcc
     2701 tcttcatact ttcgagtacg cttatactct aactgaagca acttcttttt taaacctcga
     2761 cttgattcag cttcctgtag gactaaatca tattctatgt tgtggtcttt taagatatac
     2821 ttaccaaatt gtgttttata cttctcccgt atgtattgac cataactttg catattcaga
     2881 tagtcaataa aaatgatatc ccaatatgca ttgttagaaa atttttcatc tattaactgc
     2941 agcatttttg aatcataaaa ctttgaaact atataaggtt ggcgtagaca ataccctatt
     3001 gctaaagctt ctaccaattt cattggatta tttcttatgt gtacatcatg tatcacagga
     3061 ttttcaaatt ttacactatt aaatttctct cgaaaaaaac tgacaccttc ttcaacatac
     3121 tgcggttctt cggtgaaaga caataccgtc acatcgtagt ctctagataa aacactgata
     3181 ccgttgtacg caccaatttt tccgccatta tctagaggat atggaaattg agttgttata
     3241 aatagtacat tcataatttt atcctttcaa tatgggctta gatattttat ctattcccaa
     3301 aaaatttatc ttccaacatt agacaccaac agtggtaaat tggcaaatgg tactcctgaa
     3361 tcatatatgt ttctgtttca ggagctttaa cacaaacatc tgctaccctt gcaagttctc
     3421 cacctttggc tccagttaag ccaattactt tcataccaag agctctagca acaacagttg
     3481 catttatcac atttttgcta ttgccggatg tcgaaatacc aaggaataca tcgccaggtc
     3541 ttccaaatcc aaacaactgc tgtgcgaaaa cacctaggcc atcaacatca ttaatatacg
     3601 cagtggaaag cgcttcatga gcaacgagag gaatcgccat caaaccatat tcaagattct
     3661 tagctaattc agaacccttt acaggatcaa ccgcaatcaa tttatctgca aattccctac
     3721 taacagggcg aggcgtctta aagcttttca tcagctcacc cgcaatatgc tcggaatcag
     3781 cagcagatcc gccatttcct gctatcagca gcttgccatc atttaaatag cattcctcca
     3841 taatcaggta tccgtcaaca atatcctgct tgattacttc aagagtggga tagcgtttaa
     3901 tcagcaaatc aatgtgtttc tgtaatctat tgtctatcat tgtttatact ctcctttcgt
     3961 gataaaacat cccttagcag atcttatctg caaattcact cagggttgct atggtcatat
     4021 cctgtccata ctcactctcg ccaaccaatg cagtcttaca gccggcggct tggcccgctt
     4081 taatatcatt ctcgccatcg ccaatcatcc aagactgact caaatctatg ttgaagtccg
     4141 tagctgcctg aattaacatt ccgggctttg gctttctgca ttcacaatcg aattttagct
     4201 ctgggcgctc ttcctcatat cccttatgtg gatgatgcgg gcaataataa actgcatcca
     4261 aataagcgcc cttatatcca agaagtgtct ccattttgtt atggatctct tccaattctt
     4321 caaaagaaac ttcacctctt gcaattactg gctgattcgt tattacaata gccagatatc
     4381 cagcggcatt gatctttttc actgcatctt cagtaccctt gattagttcg aactcatcaa
     4441 tatgacgcag aaagccaaca tattgattaa tcgttccatc acgatcaagg aaaacagctt
     4501 tctgcttatt ctgaagattt ttgcccttca ctcttccagc cttaaaatct tcacagacag
     4561 cataataacg atcaggggta cccatgtctt ttacgtattc tggactatca tagcagaaca
     4621 tttttccggt tcctgccaac ggctttaata actgacggtc caagtcaatc ttaggtgtgt
     4681 ttatctcgac atcaagaaca gcaggactaa tcacatggag tcccgcattt actctgttac
     4741 ggtaataagt tggacgggaa tcttccttta caagccatct gtctacagag ccatccttat
     4801 ctgcaatgat tagaccgctg tcatatggat gactgttagg gtgagtaaac aatgtcacca
     4861 agccaccctt ttcacggtga tatgctacaa atcggttgaa atcaacgtca aacatcgcat
     4921 ctgcattcag caggaggaag tcttcggtca aattatcctt taaacgaaac aatgcaccgg
     4981 catttcctaa tggctctttc tccaaaaagt attcaatttt aacgtcaaag ggctgaccag
     5041 taactggtga aattccagaa ccatcgccaa aatagttcat aatgatatcg ccaagatggc
     5101 taacggtaag aataatatct gtgaatccct ggtctctcag acattcgatt tcgtgctcca
     5161 ataccggttt gttttcaatt ttaatcatgg gctttggaat atcgcttgca accgatgaga
     5221 tgcgagttcc cttaccgcct gccataataa caactttcat attcttatcc tcgctgattt
     5281 ctttattttg gaatatacat ttccggtgtg taatgaataa cctgtgttcc accgttttca
     5341 aattcaaacg gaatatacat caaattactc attgccgaac gcacagcatt ttgtttttca
     5401 ggctgaacat aaaaaaccaa gaaaccgccg ccacctgctc cgagaagttt accgcccaat
     5461 gcaccagcag ccatgccctt ttcataaagt tcatcgatac ttccagtgct aatcgcacca
     5521 ccggtagcct ttttcaatct ccaagtgaga tcaagcagtc tgccgaaatc atctagatct
     5581 ctgttcttat cagtcagaac cgcttctgct tcatccacaa gctcatacat ttttttcagt
     5641 cttactcttt tgtcctctgt gccgctgaca ttatttgcct tctgaacatc agaggaaaat
     5701 ctggtgaatc cagtaaagaa catcatcaag ttgttgttaa gctgcttttt acgctcaggc
     5761 gtaataataa taggcaatac ttcatagcca tctgagttga aattgattcg gttaaagcca
     5821 ccataagctg ctgcaatctg atcttgccat ccgcctgctt cattacaaag gactctttca
     5881 agataaattg ctttatcaac gagtttcttc ttgtcagcgt acttgccctt caaagcatag
     5941 aaagcattca acatacctac ggcaaaagaa ctagatgtac ccagaccaga acgtgcagga
     6001 agatctgctt catatgtaag gcgaatttca tgcatatcta gcattttcat cgcattacga
     6061 attgcaggat gctcgatatc ttcaatattt gttactctct cagtacgaga atatgataac
     6121 tctgtgctgt aatcaaaaaa tctaggcaaa tgacgcacat tcacataaca gtacttatca
     6181 aaagtagtgg aaagcactgc accgccattt tctcgaaaat aatcttccat atccgttccg
     6241 ccgccaaaaa aagacatgcg aaaaggtgtt tttgtaataa tcatacttca ttgtcctcat
     6301 tgaatatagt ttattttttc cgatatttta gtgctaattt aaagattttt aaatcatcaa
     6361 ccaaatatcg ttttgccatt cttttagggt cctgagtaat tctaaacagc cactcaaatc
     6421 cataatctga catccatttt ggtgctcttt taacattccc tgcctcaaag tccaaactag
     6481 ctcctagacc aaaagacaca ggtactccca atctatcaca gttataaaac ataaattttt
     6541 cctgtttagg gcaacccaaa cccacaataa gcatatctgg atttgctcgt ttaatttggg
     6601 aatcaatttt aaacatttca tcttcgtctt tttcgaaccc ataaggagga gagtatgttc
     6661 ctacaacatt taatcccgga aatcttttca tcaggttatc tgccgcttta gccgcaacgc
     6721 cctctgccgc acctaaaaaa tacattgtgt atcccttttc tgcagccatc ttgcacagta
     6781 aagaaaatat atcggatccc gatatttttt cttttattgg tgtcttataa aactttgaaa
     6841 tccaaatcaa tggtttacca tccgtcaaaa ttaaagatgc gtttttataa acatcttgca
     6901 actcttttcc tgtttccaac tgaacaatgt gatccacatt cggagttaca acatatccgc
     6961 atctcctatc ttgaatcatt ttttcaacct ctaccaatac ttcacccatt gtaagattgt
     7021 caatttctgt gttcataaat ctcattcgtg acattagata tcctccctct atccccatct
     7081 aattttcagt aagtccgcat cgctcaacac ttaacatctt ccaatattcg ttggcaaaaa
     7141 catacaatat cttttttaca aatctaatga gtttcataac accatttgcc gactcaactt
     7201 gtccgtcccc cgatatacat aaaagaactt gaaaagattc ctcttttacc gagaacgaaa
     7261 accccgtctt tacttggatt ctttcgggct cacagtattt acatctacac agtatttcac
     7321 gagagccacc aggataaaaa tgaatgagct tatttctttt ctccaatttc aaagtggctt
     7381 ccatattcat tacctgaatc gctttttcaa agtacaattc tcacttatta tcattcttat
     7441 ctactcgatc ataatcgtaa actcgatagg taacatttga gctttcctgt atttcagcca
     7501 ctaatactcc agcgccaata ccatgaactg acataaaaaa gatctccctt gtgtacttct
     7561 tctttatata aatgtttccc aagtgatctg tccatcacag catttcgtaa catttcttct
     7621 gttacatcgt actcaaaagc ataaatcacc ccccggtgta gcttccaata cataccacaa
     7681 ctcggtttta ccattctgtc cttcatattt gatatttccg tacgtagtta tcgtctagat
     7741 gaactaagca ttcccacgta taagcttaat ggtgttaagt caattttttc caaattccgt
     7801 cttaaaccgt tctccacccc aaagataatc tttacccacg ggttgaaatt ttagtatctc
     7861 cataataact acctcgcgaa cttttagttc aaagaactct tatacttgtc agcgacgcta
     7921 atagtttcac caatctgaac ttcgataata tccagatttg tcacggcttc gattgtatgt
     7981 ttacatcctg cggcaatagt caccacatca cccgcacgaa gaatctgctt cataccatct
     8041 acaatcgctt taccctgtcc tgctacaaca gtccaaactt catcgcgaga attgtggtag
     8101 tggtagctca acttctgtcc ggcctgcata gaaactttaa cagtcatgga gccttcctga
     8161 acgtccataa cggtatatgt accccaggat ttttctgcaa acattacttc cgtactgatt
     8221 ttctcaacat acggcttcat atagccactg cgctctttat cagaaatcag aattccatcg
     8281 ccacttgctg caattaccat atctttgcag cccatagcaa gaataggaat attcaactca
     8341 ttcacaatct gagtattaac acaggtatca tccattacag ctttcccctt aatattatca
     8401 gccataactt cagccatcat gttccaagtt ccgacatctt tccaatcccc gttataacgc
     8461 atcacttgaa tgctcgtttc tttttcgacc acagcatagt caaagctgat cttggttaaa
     8521 gtctcatact tggaatacaa atcacgataa tcttcaaaat ccaccattgc gtgagctttt
     8581 tccaacaggt aaccaagttt gaacgcaaat acaccggcat tccaaacagc attctgagct
     8641 aaatatttct ctgccgttgc tttgtcaggt ttttccttaa actccttaac agtacttact
     8701 tcgttagaac tttcagggat gatataaccg tacttttcac taggatatgt aggtgcaata
     8761 cccatcaaag tcaaattagc cgtattctgc tgcaccaact tctcaagggc ttcaacgcct
     8821 tcgtaatagg aattatctac ataaggatca acaggacaaa ccgcaacact ttcatcatca
     8881 cctacgccaa gttcatcgtg aagatatgct gcagctaaca aaattgcagg gaaagtatct
     8941 ctacgacaag gttctacaca aatcgaagcc ttatctccca actgattatg gattgcacta
     9001 gcctgagact tacttgttgc aatagtgacc tttgcatctg ggtcaacagt tgtaatctgg
     9061 cgataaactc tttgaatcat agattcatat tcgccatttt catccttaaa cagtttgatg
     9121 aattgtttgc tacgaatatc gttggacagc ggccataacc gcctaccgct accacccgat
     9181 aataaaataa tattcacata tttcaactcc taatactata tataatcatt caccatatcc
     9241 aaataataca gcctgtaaag tcaagaagat aatcttccag tccgtgataa agcttctttc
     9301 attgatgtac ttcacatcaa gttccaccca ttcttcaaag gacagttcat tacgatgtgg
     9361 agcaatctgc caatagcaac taagaccagg ctgtacgaac aaccgctgcc tttcgtagtc
     9421 accgtattgt tccacctctc tcggtaaagc aggtctgggt ccaataatac tcatatcacc
     9481 tttaagaatg ttccagagct gaggtaactc atcgatactt gtctttcgaa taaatttacc
     9541 aactctcgtg attcttgggt catctttaat cttgaagacc ggaccatcca tctcgttttg
     9601 tgccaacaag gcttctaatt ttgcttcagc atctacacac atagatcgga atttataaaa
     9661 cttgaacagc tttccatttc ttccaactct atcctgcaca aaaatcgggc tgccaccggg
     9721 atcgtcaatc acaatggcga gcgctgtaat tagcatcaca gggctcaatg caatcaaagc
     9781 tagagccgag aagaagatat cttgtgctct gcgtccaatc caatagaatc tatgtcccct
     9841 aagattctct tccctatcaa ttgttacgtc tttctgaaca cctttctttt cttctatacc
     9901 aatcatcttt ttccattcgt ccctctattt ttacaagtct catcatctgt aaacgactta
     9961 aaggcctgtg atcatcgacc actctatctg aaacagttct catcgaacca tgttcattcc
    10021 tactaaaagc agccaactcc aacgccggca cattcaattt tcatatctat ctcctcctcg
    10081 tttaggggag aaatacgagt ttaggtcatt tttattgacc tcgtttgggg ctatattaga
    10141 atttcacatt gaaatgtact tacattctca acaccatcat tttccaaaaa gtattatgta
    10201 ggtacatatt gacctctaat cccaccccaa aggcaggttt gaaattcaat gattgagcta
    10261 ttctcacccc aaagatgagt ttatcgaaca atttattggg ctattactac cccaaactgt
    10321 ctatttacag caaatttatg attttataca ttgaaatttt ccaatccgaa aaacccaaac
    10381 taattttctg cgttgtagac attggtaaat acttgaacaa cttcacggct atcatcatca
    10441 catcctacga taataattct aattggataa agattatcat caataattta cttccacgta
    10501 aaaattctga actaaataaa atctattgat aatatttttt ct
//
LOCUS       DACENJ010000085        10358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.085, whole genome shotgun sequence.
ACCESSION   DACENJ010000085 DACENJ010000000
VERSION     DACENJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.085"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..507
                     /locus_tag="IW748_001557"
     CDS             <1..507
                     /locus_tag="IW748_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104472.1"
                     /translation="TTYGKSFRYLPFNLCDFLVYGETSELLKLYGIQRDNRASDFGKI
                     IYTEEKEFNKKIFTLLEGKQGVSPYDVFPDFDALYKKYMFAEYYIVYNYFLENIASNE
                     SYGDLMDAYYSYLKKYAVVADSEKLLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIY
                     ANYTPFSQ"
     gene            533..1618
                     /locus_tag="IW748_001558"
     CDS             533..1618
                     /locus_tag="IW748_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAQ1104473.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            1641..2456
                     /locus_tag="IW748_001559"
     CDS             1641..2456
                     /locus_tag="IW748_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104474.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            2440..4158
                     /locus_tag="IW748_001560"
     CDS             2440..4158
                     /locus_tag="IW748_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HAQ1104475.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            4184..5020
                     /locus_tag="IW748_001561"
     CDS             4184..5020
                     /locus_tag="IW748_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1104476.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            5025..6458
                     /locus_tag="IW748_001562"
     CDS             5025..6458
                     /locus_tag="IW748_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1104477.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILRENGIKEDMYNIKNIIFLSVLMIVLTIGMTILYFSTILR
                     YSIIIGLVIVAFLKRTQIMKILKNIKN"
     gene            6475..8136
                     /locus_tag="IW748_001563"
     CDS             6475..8136
                     /locus_tag="IW748_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1104478.1"
                     /translation="MERRHGEMRLLIPSAKIVPEELHHLGKLPAIIYPINQKIVFDYL
                     YDQYKDVCSAIDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCD
                     EPVIINFADTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNELKEDD
                     GKKHKLFSGVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNW
                     FDIGHADKYYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVE
                     YVRPRIFEYSTSYINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCSDFKR
                     YSVSGDNIQKSLKDMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEALL
                     SVSVPRELFDITQFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYELA
                     KLFHSVDGKYDFIIKDLFTIKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEIGSDL
                     KKIELIEALLFLSMIPLHGESLNHQMAMLATGLEILGRVVPDIYC"
     gene            8224..8622
                     /locus_tag="IW748_001564"
     CDS             8224..8622
                     /locus_tag="IW748_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1104479.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            8647..9363
                     /locus_tag="IW748_001565"
     CDS             8647..9363
                     /locus_tag="IW748_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104480.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            9578..10222
                     /locus_tag="IW748_001566"
     CDS             9578..10222
                     /locus_tag="IW748_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104481.1"
                     /translation="MCSGEVTSAKLSKQIYRVLKIYIIWSILYIPLVLKNWVQSDFSW
                     KTDVLLYIRDFLFIGSYYHLWFLPALAFALWLVRKSQVSKIKFLSGLLLCVVGLAFES
                     YWFILSGNDSIEYIRYCYKSVFVTTRNGLFIGVPCVIIGQHLAFHKNTILNKVFKYRN
                     FILCAVLAIGCLLVGESFIINQIALREVNNLLIFSIPLALVVVSVSLSLKIVHL"
BASE COUNT         3405 a         1355 c         2037 g         3561 t
ORIGIN      
        1 acgacttatg gaaaatcatt tagatatctt ccgtttaatt tatgtgattt tttggtatac
       61 ggagaaacta gtgaattatt aaaattatat ggtattcaaa gagataaccg agcatcagac
      121 tttgggaaaa ttatttatac tgaggaaaaa gagtttaata aaaagatatt tacactacta
      181 gaaggaaaac aaggtgtatc tccatatgat gtttttccag attttgatgc actatataaa
      241 aaatatatgt ttgcagaata ctatattgtg tataattatt tcctggaaaa tattgcaagt
      301 aatgaatctt atggtgactt aatggatgcc tattatagtt atttaaagaa atatgcagtt
      361 gttgctgatt cagaaaaatt gttactatat tggccgaaat atgttcaata taatgttaag
      421 ttcgaaaatg aacttgattt ctatgcgaag ttggattcaa aaaaatggat ggatatttat
      481 gctaattata caccttttag tcagtgatta tatggaaagg atgaggtgaa taatgaaaat
      541 cggaatagtg actattgtta atggggcaag ttttggtaac agattacaaa attatgcgac
      601 tcaaaaagtg ttggaaaata acggctatac agttgagaca attaataatt gttttgacaa
      661 agaaagtacc ctatccattg ttatcaaaaa cgctatttat acattattta agtttatacc
      721 gcatttcaaa aagtcaaaaa gtatctggaa aagaatgtat acattttatc aatgggacaa
      781 gcagtatata gattattctc atcgtaaaat tgatgcatct aaaaaaaaca gctttgtgag
      841 tataaagaat gattatgact atttcttggt tggtagcgat caaatttgga atccaaaata
      901 tgcagccaat aagggatttg aacttttatc atttgttcca aatgaaaaaa gagtttcatt
      961 atcaaccagt ttgggagttc catctatacc aagtgattta gaaaaattat atgtgacaga
     1021 gtggaataag tttaaatcaa tatcggtacg tgaaaatcaa gcggcaaaaa taattgagga
     1081 cttgacaggc acaactgcgg ctgttcacat tgacccaact ttaatgttgg agcgttcgga
     1141 atgggaagcc tttcaaaaaa aaccagacaa tattgatgaa aattacatct tgtcgttttt
     1201 cttaactaat ccgcctaagg atactgttga tgtattagat aaggtcgcta aagatttgaa
     1261 aataggtgtt ctcaccttag atgcgtcttg ggaatatttt caagatatta ctccgaatca
     1321 ttttgtttat ttgatttcac atgcaagaat gatttataca gattcattcc actgcacagt
     1381 tttttcattg ctatttggaa aaccggttgt ggtatttgac cgttttgata gtgaaaaaat
     1441 gaattctagg ttaagtacat tgttaaataa agtaaataaa gcacattttt gggtaggaaa
     1501 aaaagctgat gaagcaatga ttcgtgcaat agcatctgaa cctgaatatg acatagataa
     1561 gctgatggta tcagagaggg aatgtgtaaa agcttatctt aaagagtatt tacagtaaaa
     1621 ttttgaaatg ggatgaaaat atggagcatt taagtgattg ttttggtaaa atgaagattg
     1681 ttacgacttc atgcgcaaga gaatatttta aggatgattt ggataaatca gagtattcat
     1741 gttttgttga ttctcgagca gctatgtttt tcgcaatggg acaagcttta gctaaaagaa
     1801 ggaatgtagt tgtaatgctt gctgatgaat tgacatcgtg ttatacggca ctaacagaat
     1861 gtgcatttca acaagttgat ttaattgtgt ttgcttttca gacagtcaga catgaaattg
     1921 atttagcatc tttgaagaat tgtggttgca atctaattaa ctgtagcgcc caagacgata
     1981 ttgatagagt tgtgtccaac actaaggggt taaatcagac aacagtaatt aaaatcaata
     2041 tgaattgtga gtcggataaa gatcatatat gtgagtatat agacctgcca gacatacctg
     2101 tttttttcca cggtagctta tactcacctg caagtaaaaa gaaatatgag cagtatggac
     2161 aggtatcgaa gtatttaggc tatctcgtgg ggggaaaaga tgcaatactc ataatttctc
     2221 acaatgagtt tttaaacgat atcagtgtat ggattaatta ttattctttt ggagaaattc
     2281 aacccaaaat tattatattt gacagtccga atgactatca atataaatgg ctgtgtgact
     2341 tagaatgcag tgtagctcat tcaaccaacc gatttttgtc tgaggattta gccttattgc
     2401 aaaataacgg aatttccatt gtgttaagag gtgataatta tgatatcgtc agttaaaaat
     2461 gttcaaattt tagtatcttt attaaaacag cataatatta aagatatagt tatctcgcca
     2521 ggtagtagag attttcaatt ggtgcatagt gtagaaacag acagtttttt tagaacctat
     2581 acggttgtag atgagcgcag tgctgccttt tttgctaatg gactttctat ggcgattgat
     2641 aagcccgtgt gtgtatgttg tacttcttca actgcctctt caaattatat ggcggcagct
     2701 aaagatgctt ttgctaatgg catccaactt gtacttctta ctgctgatag agattaccgc
     2761 cgcttatatc agatggaaga tcagatgatt gatcaggtag atatgtatgg ggcttatgtg
     2821 cgttctaacg ttgatattcc tattgttaag gattctgatg atgaatggta tgctaccaga
     2881 aaaataaatg aagcaatatt ggagttaaat catggcgtta agggtcccgt gcagattaat
     2941 tatcaattaa tggatttaag tgcgccgttt gttgagacca tgccagagta tcgtaaaatt
     3001 acaagatatc aagaggatga tttactgtca tcagtagaga tttttaaaca gaaactatta
     3061 tcaaaaaaga gaataatggt tttatgtgga cagcaggaag aaaataattt aagtgcctta
     3121 ttggaagagt tctttattaa atataataca attataatca aggataacta ttctaatata
     3181 cacggtgaac actttattaa gactgttcta gtaacagaaa gaatgagtgt aaatgcattt
     3241 attgattatt gcccagattt ggtaataact attggtaaac atgtatggtc ttttgtgaaa
     3301 tatactctta gagataaaaa gcttaaattt gagcattggc ggatatctga ggatggtaag
     3361 attgttgatc cgttcaacaa gctaacatct gtctttcagt gctcagcgga attttttatg
     3421 gagactgtca ctgctggtgt gaatagcaat aataatcttg agtattataa tctatggaag
     3481 acaagattat caaaagtttg ctatccagag ttagtctata gcaattttta tgcaatttca
     3541 gaactagtaa aaaaaattcc agatagtgca ttagttcatt ctagtatttt aaatgcggct
     3601 agattgtatg actttgtatc tgatgataaa gcattactca gttttgctaa tttaggggct
     3661 gatggtattg atggttgttt accaacattt ttaggtcagg cagaagcaga taaaactcga
     3721 cctgcgtatt taatttcagg agacttgagt ttcttttatg ctatgaatag tttatgtggt
     3781 gacattccag ataatgcaca tattttcctt attaacaatc attctggaag tgaattccat
     3841 acaaattttg gtaagtttta caacggatca ttaagtgttg ataagcatat agctgcagga
     3901 catatttcaa acgcaaagga ttgggcatta gaaaataata taaaatacat tactgcggat
     3961 tctaaggaaa gtctcaaatc ggggttggat gtatttatta atacatttga cagtcctgtt
     4021 ttatttgagg tctttacaga tccggatacg gatacgtctg ttctggaatc tttttatagt
     4081 ataaataagg taatcacacc aaaaatgtat tttagaaagg cgttagatat tataaaaaag
     4141 catttacaca ttaagtagat gtttattgcg aggtgaaaca aatatggcaa taaaatctta
     4201 tattaaacgg ttattgcttt atatccgtca tggagatccc aaaataaatt tagatgtgag
     4261 ttatttatct gaaggtgcaa tgcttgctgg taagactgtg ataattacgg gtggtgcaaa
     4321 aggacttggt tttgcaattg caaaatcatt tatagctcaa ggcgcaagcg ttattattac
     4381 aggaagagac atggattcgt tgaataatgc taaatgtcag cttggagata tgtgttctat
     4441 cagggcgttt gatataactg atgctaaaat gtatcgaacg ttttttgaag atgcatacga
     4501 gacctttgga cgtattgata tactgcttaa taatgctggg atctcgttac acgaagatgg
     4561 atttatgagt gttactgaga aaacttggga taagcaaatg gatacaaatt tgaaaggtgt
     4621 ttatttcttt acacaggaat ttattaagtt tgttaaagaa aagaatcaaa tgcaagcaaa
     4681 tattattatg atgtcctcag aaagaggctt gtgtggtgat agtattccgt atggattatc
     4741 aaaggctgca attaactctt tagttgtagg cttgtctaag aaatatattt ctgagggaat
     4801 tagggttaat ggtattgctc ctggagtaac tgcaaccgct atgactggta tttctgaaaa
     4861 gggtaatttg tataatcctg caacgatcgg ttatagatat tttctcccag aagaagttgc
     4921 tcaagttgct ttgtttttgg caagtagtct ttcaaactgt atcagtggtg aaatcatagc
     4981 ctgtgaccag gggcaatatt taagtcgaaa ttgggggtaa taacttggga atattattaa
     5041 aaaaatataa tagccttagt gagcctgcaa aagcatctat ttggtttaca ctttgtaatg
     5101 tgttgcaaaa aggaattcta tttttgacta cacctatttt tacaagaatg ctttcaactg
     5161 cagaatatgg taagtatacg atttttatat cgtggtattc tattatagca attttcacaa
     5221 cgttgaattt atctgcaggt gtctataata aaggactaat taaatacgag aataaatatg
     5281 cattaacctc gtcattttta ggattatcgt catttatttc cctttgtgca ttgatgctat
     5341 accttccatt tagaacggtt ataaatgggc tactggattt atcgacggag tttgtcatat
     5401 tgattcttat tgaatcaata tttgagccgg cttttttgct ttggtcagtt aaggaacgat
     5461 ttgattacaa gtatcaaaaa ctagtactca tcacagttct tatggctgtt ttaaatccag
     5521 gtctcggtat tatattaatc ttatcatttg gggagtatgc tatttggaga gtacgagctt
     5581 atgtcgtggt aaatgttgta gtggggatgt ttattggcat aggaatactg gctaagggta
     5641 agaaattatt tgataaagag tactggacat atgcgctcag atttaatttg ccattaatac
     5701 cacattattt atcaatgaca ttgctgaatc aggttgatcg ccttatgata aatagtatgg
     5761 ttggagctag cgaggctgcg atttatagta tcgcttacac tatatctagt atgatgaata
     5821 tcataacaac agctataaac ggttctttta ttccttttac atacaaagca attaaagatg
     5881 gcgtgtatga aaaaattaaa tccgttgcta atcaactatt agtgttgatt tttgtcttgt
     5941 gttcgtttac tatgctgttc gggccagaat tgatttgggt tgtcggagga gaaaaatata
     6001 tggatgctgt ttggattatt cctccagttg cggcatcagt atttttcatg tttttatatc
     6061 cgctttttgg caatattgaa ttttactatg aaaagacaaa ctacgtaatg tttgcatctt
     6121 gtgtgggctc tatcgcaaaa attatttcaa actatgttag tattccccgt tttggatata
     6181 tttcggctgg ttacacgaca ttgatttgct atatgttgtt ttcctttgcc cactacatgt
     6241 tttataaaaa aattcttaga gaaaatggta taaaagaaga tatgtataat attaaaaata
     6301 ttatatttct ttctgtctta atgattgtgt taactattgg tatgacaata ttatactttt
     6361 ctacaattct aagatatagt attattattg gattagttat tgtggctttt cttaaaagga
     6421 cgcaaataat gaaaatcctt aaaaatataa aaaattaaca agcaaatctt gggaatggaa
     6481 cggaggcacg gtgaaatgag attattgatt ccatctgcta agattgtacc ggaagaactt
     6541 caccatcttg gaaaacttcc tgcaattatc tatccgatta atcaaaaaat tgtatttgat
     6601 tatttatatg atcaatataa agatgtatgt tctgctatcg atattgcctg ttacgaaaag
     6661 atggataagg tagcaagacg attagataag tatattaaat ctaaaacagt aaatattatt
     6721 cagcttaaag agttagggga tcttggaaga actatatatg attctctaat aggatgtgac
     6781 gagcctgtta ttattaattt tgctgatacc attatcaatg ataatatata ttccttggaa
     6841 tgtgacagct ttttctatgc tgaagactat tatagtaata cttggacatt ctttgaagaa
     6901 aaagacggag atattatttc tgttttagat aagaacgagt taaaagaaga tgatggaaag
     6961 aagcacaaac tgttttctgg tgtatttcaa attatggatg cccagtattt tagagagtgt
     7021 ttgcgtaaag ctttgatgtc aaatgtagta aatgtgaata gtttttatca agctttacaa
     7081 gagtatagca aacgatatga atttctctct attaaaacaa ataattggtt tgacattgga
     7141 catgcagata aatattataa ttccaaattg gaagttaagg caagagaatt taaccacatt
     7201 tcaattgata aagatcgtag catcctgaga aaaattagtg aagatgttga aaaatttatc
     7261 ggagaaatta agtggtatct taagttgcca gcacaggtag aatatgtgag acctagaata
     7321 ttcgaatatt ctacttcgta tattaatcca tatgtaagta tggaatacta ttcgtatcat
     7381 actgttcacg agttatttct ctatagtgac ttaacaaaaa aacaatggat agatatcttt
     7441 aatagaatac gatttgtatg ctcagacttt aagagatact ctgtttcagg ggataatatt
     7501 caaaagtcat tgaaagatat gtatctcgat aaaacatttc agagattcaa taagctcaga
     7561 aaggaccccc ggttcacaga attcttttct tctgatattc aaataaatgg agtcagatat
     7621 aaatctttgg accagataga agctttattg agcgtaagtg ttccaagaga attgtttgac
     7681 attacgcaat ttaatattat tcatggtgac ttatgctttg ccaatattat ggtagataac
     7741 acattttcgt tcattaaagt catagatccg cgaggtaagt ttggagattt tgatatttat
     7801 ggtgattata gatatgaact ggcaaaattg tttcatagtg ttgatggaaa atatgacttt
     7861 ataattaagg acttgtttac aataaaatat gatccaaaga aggcaatcat agactatatt
     7921 gttcaagatc gtaaacgaga ttatgattta tatgaagtat tttactctgt cttcaaagat
     7981 gaaataggtt ctgatttgaa aaaaattgaa cttattgagg ctttgctgtt tttaagtatg
     8041 attcctttgc atggagaaag cttaaaccat cagatggcta tgttagcaac tggtttggaa
     8101 attttaggac gagttgtccc ggatatttat tgttaatgct ttgagctatt gtttttctat
     8161 ttgatcagtg taaagccggg catataaatt gcctggctaa aataaaggag atgtcttagt
     8221 tatatgttta aagattacgc atttgttttt gatattgatg gaactttgtg ccccatcaaa
     8281 aagaaagatg agaaatatga agatttagtt ccgtatgcta atatggtgga gaagttgcga
     8341 tactataagg agaacggagc aaagattaca ctgttcacat ctcgcaatat gaattcttat
     8401 catggtaatt taggtgtgat caatgcaaat actgcaaaaa tcttgttggc ttggctagat
     8461 aagtgggaga ttccgtatga tgaaattctt tatggaaagc cttggccagg ccataaaggg
     8521 ttttatgttg atgatcgcac agttcgtccg gatgaatttt tatcgtgtac cctggatcag
     8581 ctcgatgaga tttgcaaaaa atccaaattg aaatctgagt aaaggagact ttataaagtg
     8641 aataagatga cggttattat tacaatggct ggtcttggca gtcgctttcg taaagttggc
     8701 tacactgtgc ccaaatttat gattgaagca aaaggtaaaa ccttatttga ttggtcctta
     8761 gacagcttgc ttgattataa ttcacatgta gaaaagtatg tttttgttgt gcgtaaggaa
     8821 gatgatgcta ctgtttttat caaagaacat tgcagtctgt atggaattga aaatgtcgag
     8881 gtagttgaaa ttgactatat gactgatgga caagccacta catgtatgct tgcaattcct
     8941 tattgcgcac ctgataatgc aattatggtt tataacatag atacatatgt tgagccaggt
     9001 gagatgaaat acgttgatat ttctggagaa ggacatattc cgtgtttcca tgcggacggt
     9061 gatcattgga gttttgccaa gttgaacgaa tatggtgatg tagtagaagt aagagaaaaa
     9121 gttagaattt ctaacaattg tactttaggt gcatattatt tttcttctgc aaaattatat
     9181 gcatcactct acgaagaata ttatgcagat gaaaggcatc tggaaaagaa tgagaaatat
     9241 attgcgccgc tatataatta tatgatagag aagggcatga aggtaaccat tagtatagta
     9301 gataaatcaa aagttcacgt actcggtact ccagaagagc tcgatgtgtt cttaaatgaa
     9361 tagggaaaat ataatcatag aaaatgttaa ttataaagct gcgtgtgtgg gggagtacta
     9421 ttcgatagat attctaaaaa tactaatggc tattttagtt gttgttcgtc atttaggtca
     9481 aaactattta gctccagatt aagcatttag tattggtatt gttgcaggac tgagtacggt
     9541 tgccgttcct acttttttca tcatcagttc ctttttgttg tgtagtggtg aagttacatc
     9601 cgctaaatta tctaaacaaa tctacagagt acttaaaatt tacattattt ggtctatatt
     9661 atatattcca ttagtattaa agaattgggt acagtcagat tttagctgga aaacagatgt
     9721 attattgtat atcagagatt ttctgtttat tggtagttac tatcacctgt ggttcttgcc
     9781 tgcactggct tttgctctat ggttagtacg caaaagccag gtttctaaaa taaagtttct
     9841 cagtggtttg ttgctatgcg tagtaggttt agcatttgaa tcttattggt ttatattatc
     9901 tgggaatgat tccatcgagt atattagata ttgttataaa agcgtgtttg taacaactcg
     9961 taatggatta tttataggtg taccatgcgt cattattggg caacacttag ccttccacaa
    10021 aaacactata cttaataagg tctttaaata ccgtaatttt atcttatgtg ctgttttagc
    10081 tataggatgc ttattagttg gtgaaagttt catcataaat caaatagctt tgcgagaagt
    10141 caataatttg ttaatatttt ccattccatt agcactggta gttgtatcag tatcactgag
    10201 cctaaagata gtacatttat aaaaataaca gtacatctgc gaagaattag tacgttatta
    10261 ttttgtttac atccgtatat agtagctgct ttaaatttta ttttcccaaa attggggatt
    10321 acaaattcat taatagaaat gacttgtgga atgataat
//
LOCUS       DACENJ010000086        10276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.087, whole genome shotgun sequence.
ACCESSION   DACENJ010000086 DACENJ010000000
VERSION     DACENJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.087"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..257
                     /locus_tag="IW748_001567"
     CDS             <1..257
                     /locus_tag="IW748_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1104482.1"
                     /translation="SQTIASKIQSMLEDDGIAFPVEAVDYKSIQNELLTAGIYVYVAQ
                     PDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            330..1691
                     /locus_tag="IW748_001568"
     CDS             330..1691
                     /locus_tag="IW748_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1104483.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1713..1973
                     /locus_tag="IW748_001569"
     CDS             1713..1973
                     /locus_tag="IW748_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104484.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            1987..2739
                     /locus_tag="IW748_001570"
     CDS             1987..2739
                     /locus_tag="IW748_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1104485.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2833..3438)
                     /locus_tag="IW748_001571"
     CDS             complement(2833..3438)
                     /locus_tag="IW748_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1104486.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3491..4465)
                     /locus_tag="IW748_001572"
     CDS             complement(3491..4465)
                     /locus_tag="IW748_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1104487.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4843..5622)
                     /locus_tag="IW748_001573"
     CDS             complement(4843..5622)
                     /locus_tag="IW748_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1104488.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5689..6960)
                     /gene="celB"
                     /locus_tag="IW748_001574"
     CDS             complement(5689..6960)
                     /gene="celB"
                     /locus_tag="IW748_001574"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1104489.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7113..8510
                     /locus_tag="IW748_001575"
     CDS             7113..8510
                     /locus_tag="IW748_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1104490.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8591..9031
                     /locus_tag="IW748_001576"
     CDS             8591..9031
                     /locus_tag="IW748_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1104491.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9179..9505
                     /locus_tag="IW748_001577"
     CDS             9179..9505
                     /locus_tag="IW748_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1104492.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9506..10255
                     /locus_tag="IW748_001578"
     CDS             9506..10255
                     /locus_tag="IW748_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104493.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3254 a         1817 c         2128 g         3077 t
ORIGIN      
        1 cgagtcagac aatcgctagt aagatccaaa gtatgttaga agatgacggc atcgcatttc
       61 ctgtagaagc agttgattac aaatcgattc aaaatgagct tctaactgct ggaatctatg
      121 tgtatgtcgc acaacctgat gaggaagtac ttgaacaagc aaaagatttg ggtattgaag
      181 tatttccagg aattccattt ttaactggaa tgggtgtaga acctatttat gacagtatca
      241 aagagttagt acagtaaagg aggattgttg gataaaaaag ttggttcata gttttattta
      301 ctatatttag aaagaaagaa ggtttattta tggaagtttt ccaaaccatt atcaattatg
      361 ttttgaactt aggatcagcc atttttgttc ccctgattat tttgatactt ggattgttgg
      421 cagggatgaa attaaaaaaa gcattcatgt ctgctttgac tttagggatt gcttttagcg
      481 gaatgtctat ggtcattggc tatatgtcta acgcagtaag tccagcatca gaagcattgg
      541 ctaaaaatac gggaatcagt ttgcctgcac ttgatcttgg gtggacaggt gcagcaagca
      601 tcacatggtc gtggagttat gcttttatat tttttgcagt aacgattggg gtcaattttg
      661 tactgttact ttttaattgg acgaagacgt taaatgttga tatgtggaat gtttggggga
      721 aagctttaac tgcttattta gtttattatg tttccggtag tttggctgca ggctttctta
      781 ctgcaatggt ccaagttata ttggaattaa agcttggtga tatgtttcaa aaacatatac
      841 aagatctcac agggattcca ttagtaacag tgactcattt tatgacatct gccgctgtct
      901 tgttgctgcc tttcaatatg atcatggata aaattcctgc tttaaataaa cgagcggata
      961 cgaatgcatt gaagaagaaa attgggatct tctctgaaaa ctctgtaatg ggattcatta
     1021 ttggattggg gcttggcttt gctgctgcgt atggcgtttc aggttctctt aatctagcga
     1081 tccaaacagc tacagcatta agtttgtttc ctatgatctc taaaatgttc atgcaatctt
     1141 tgtctcctct agcagacgct atgtcagaat ttatgaaaaa gcggtttaag gatcgtgaag
     1201 tatatatcgg attggactgg ccgatattag ctggtcgttc agaaatttgg gttactgcta
     1261 ttttacttgt tcctgtgttt attggttatg ccattatttt gccaggaaac caagtattgc
     1321 cattagcagg gattatcaac tattcgattg cggtcggcgg attactttta acaggaggaa
     1381 acttggctcg tatgctgatc ttgggaatca tcactatgcc tatttatttg tacggggcca
     1441 cttaccttgc gccaatactc agtgatttag cagaaaaaag cggagcagtc gagggaatca
     1501 agaaaggaca gttgatcaca tggtcatcca tagaagggcc ggagttcagg gttttatttg
     1561 cggaggcatt taataggaat attctcgcta ttgtaggatg cgtgattttt ctaggactgt
     1621 ttgttctact ttataaatat atgataaaag cgaaagtacc gagtgaacgt tatgctgaac
     1681 tacaaaaata gcattgaaat ggtgggaaag taatgaaaag agaagagaca gataaaataa
     1741 aatggacagt cgctctttgt ggtacacttc tgctatttct atatggactc tttactcaga
     1801 atatcatcat caatctattg gtcatttttt tcgcactagt gatctataaa tatggaaatc
     1861 acgttttatt cagagagtac gatgaaaaaa gaaaacggaa aatagaagaa tcaatgaaaa
     1921 taaaagaagc taccaaagaa atattgagag aaaaaagttt tataaaaaga taatggaggg
     1981 gtcattatgt cagatggacg tatgttattt gagagagaac gacaggacat ggcacgtatc
     2041 gtaagagtga ttttcgaaag gaaaaacaca aatgtcgcag gagggaattt ttcctttaaa
     2101 accactgatg atcaagggaa agactacatc atcatgacgc cgaccatgat gtcagaagcc
     2161 tatctaggag agatttctcc tgcacaggtt ctagtagtag aaccaagaac gcgtagaatc
     2221 gttgacggag tcggtcattt aactcgtgaa atcaatatgc atgaagcgat gtatgagact
     2281 aatccagcta tcaaagctgt cctacatgct catgcgccta atgcaatgtt ctgggcgact
     2341 actgggttgc ctatgcctaa tttgacagaa gccacagcta aagtaaaaga gattcctgtc
     2401 atggactttg agcctaattg ctctgaagaa cttgctgaat tgacaagcga ctatgtaaaa
     2461 aaacagcatc tcgaattacc gcatatgttg ctgctcaact ctcatggcgt attgatcaac
     2521 acaggtggag aatcaggaat cgaagctatc cataaagcac tgcaaatcct tgatacagtt
     2581 gaatggaatg cagaaattgc ctacaagcag acgatttttc aagcattagg gattactgac
     2641 ggtttttatt caaaaggtga gaaaatcggg ggattggaag agttgaaaca taaaacggct
     2701 atttataata aatctgttat tgcagctggt ggagactaac ctatctgaca caaaaaacta
     2761 cgactttcac aaaatctgat ccagatcgat atctgcttca ggttgtatta tgtaagtcgt
     2821 agttcttttc ttttatttag ttaagaggtg gtctgtccat tccctcgctt gcttgatttc
     2881 gtccatcgtc aactgatgac ctgttacagt tagctgaata gtcacatttg cttggcgtgt
     2941 ttcgaattga tcggccaatt ctttaaagga agcaggggaa acgattggat cgttatcacc
     3001 ataagaaatc cagacggttt tatcctctaa tgtggaacgg ctttgatgaa cacctagaga
     3061 catcggatgg aaaaacaacc ctttatttag ctcggtctca cgctccaata ataaatgagc
     3121 agcgatgttt gcgccatttg aatagccgac aagcagccag tcacttaacg gtatcagttc
     3181 tttttcgctg atttctttga ttttttcatg gagtttatct gtttcttttt ccaaactgtc
     3241 caggtcgaat tgattcaatc cattacgctt gaaaaaacga ttcatcccat cttcttggac
     3301 cgtgccacga aaagaaagca atgtactgtt tggtgcaaga aagtgagcga tatccagtaa
     3361 agaatgttca tcgccacctg tgccatgaag taagatcaaa tgcttgccac ttgttccttt
     3421 ttcaaaaatg taggtcattt tgttccctct tttctgtttc tatagatgca aatgaagtag
     3481 catgaagaga ttattcacgt tttttatttg catcacttgt atcgaaagga cggacataag
     3541 cttcaatggc ttcacgacgt tcttctaaga atggaggaag agatagtttt tctcctgctg
     3601 attctgctgg ttcatcttca aggaatccag gaccatcagt tgctaattca aacagtacgt
     3661 gatgagcggc taggaagtac tcagattgga aatagaaacg atcgacgaaa ccagaatttg
     3721 gcaattcaat cttattgatt acttgaatcc agtattccaa tgcttctttg tctgctacac
     3781 gcaatgctaa gtgatggata tttccaaatc cttcgattgc ttttggcaag tctgtccgat
     3841 gttcaataat gatgcttgca ccatttcccc cttcaccgat ttcaaataag tagaaatctc
     3901 ctgaatggtc tgtttctttg aatcctaaga ttttttcaag aatcaagcgg atatgctcta
     3961 attgactgac acggacaaag acaggtccaa gtcctgtgat cgcaaactct gtcggtacat
     4021 tgctgttttt ccaaggaatg ccagctgcta caccttgatt gttttcgtca gagatcaatt
     4081 gataatactg attatcgaaa tcttcaaatt cgatatattt tttgccgaaa cgttcattga
     4141 ttgctgaatg agtaacgtca tatttttcaa aacgttccaa ccaaaagtct aatgctgcgt
     4201 cacttggcac acggaaagaa gcacgagaga tcgtatctgt tccttttttt ccttttggct
     4261 gatttggaaa atcaaagaaa gtcatatctg ttcctggcgc accagtatcg tctgtaaagt
     4321 aaagatggta agtttcgatg tcatcttggt taactgtttt tttgattaag cgcattccta
     4381 aaatatctgt aaaaaagcgg tagatttttg tagcgctaga tgtcattgct gtgacgtggt
     4441 gaattcctct gatttgtgta tccatgataa atttcctcct gttaacttac aaaaagtaag
     4501 tagttgattt agacatagta tagagggtgc atctctattt tgcaattaat ttgtcttcta
     4561 aagtatcagg aaggaatcaa gaatggaaaa gaggagggac ggatcaataa aataaaaaga
     4621 cttacgacta gctgtatcga tttcaaaaaa actagcttta aaaagctaac tttagaatca
     4681 ttacgggtgg tcataagtct ttttatgctg agttattttt tcatttttga gctaatgctt
     4741 aggatagaat agaatgttga tttcacgatc tcttcttgtt acttgaaact gcttaccact
     4801 gtcacgatct cttggtaaac ggctttttca caacctcttt ggttaagttg aacgcacatc
     4861 cagcacagaa tagttccgaa catcaaatcg gtttcggctg gaagaatatt caatcggctg
     4921 tccatctttg agatagacga cttgctgaac ttccagtaca ggatcgtttt ctgcacagtt
     4981 caaatattgc tgatcatatt catcactttt atctgcagaa atagcacgat aagcccctgc
     5041 aaaatggaca ttcaattctt gatgaagatg atcatagata gaggctaaca aatgttcttt
     5101 ttttaaaccc ggcacaagat ggactggcat aaaggtatgc tctaaaacat atggctcgcc
     5161 atctaagatc cttaaccggt gaatattgta gacaggttgc tcggcatcga tcatcaatcg
     5221 ttcttggata tattgattag ggaattcaac atcaaaatga atgatttgac tcttcaaaga
     5281 acgatgtctg cgtttcattt ggagactcaa accttcatac tcgttagcgg gcgatgtgtc
     5341 tttttgcata aatggatgat tcaagatttt cgttcctgat cctcgctgcg aatagacaag
     5401 tccttccatt gctaagatat tgatggcttt tttgattgtc atccggctcg caccaaattc
     5461 ttttaccaag tcgacttgat taggcaaaaa actatcgaga ggatactctt ttgaacggat
     5521 acgttctctt aaaatatttg cgatttcttc gtattttggc acgttattca cctcttctag
     5581 agtataacga aagattatgc ttccgacaac tcgaagtagc atctggaaca ttgtttattc
     5641 gaaaaaagag gtcatgacaa gtttttgtca caacccctta atgatctatt aagcaatatc
     5701 taaatctggt tgtttatcta gtactttcag gaatgggtac cagataaagg caacgatgac
     5761 aaagtcgatg acttgcagca taccaccaag ccatgaattt gttgctaaga caccgcttgc
     5821 aatgattggc acagtccatg gaacagagac gccagttggt gcagggacaa gtccagcagc
     5881 cattaccaaa taattgaacg tcgttacgat tagaggtgcc acaacccaag gaataaggat
     5941 tgttgcattc aatacgatcg gtagaccgaa gatcgctggt tcatttacat tgaaaatacc
     6001 aggtgctaaa gctaatcgtc caacatcacg atactgtttc tttttaagaa caaaggccat
     6061 caataaaacg acagctaaag tcattccaga gccaccaata ccaacagtga aggtttccat
     6121 gaatggtttt gtgatgatgt gtggtaattc ttttccatgt tgataagctt ctaagttatc
     6181 aagcatcagt gtattccaaa tcgggtccat tacagagttg acgatgattt gtccgtgaag
     6241 tccgaagaac cataagaatt ggacaaagaa caaagcaatc aatgttgcag gtaaactact
     6301 tcccaaacca gttaatggtt tttggatcac ttcataaacg acgtcatgta agttagcatt
     6361 gaaaatacca gtcatcacag cattgattac taagaaaata gttagtgtca ctacagcagg
     6421 gatcaaagcg gcaaatgatt ttgttactgc aggcggaact ccatcaggca ttttgatctg
     6481 ccagccgcgt ttcgtgattc tgcagtagat ttctccagcc aagaaagcag cgatcattcc
     6541 aatgaacatt ccttttgcgc caagacgatc aagtgaaagc acaccagtca catcttcacc
     6601 gccagcggtt ttcattgcaa aaggcgtaag aatcaagaag ctggcaaaag acacggcacc
     6661 accgaaaata ccttcaacgt cataagattt tgataaataa tagccgatac caaaagtaac
     6721 aaaaaccgac atgatgctca ttgttgcatt ttgcccatta ccgaataaat tcgataaagt
     6781 tccctttgtt gcgtcactga aaaaaggcaa gttattgata acgacaacga tcgaaccgaa
     6841 catcgttaaa gggaaggaaa gcatgaaagc atcccgcagc actgtaaggt aacgattttg
     6901 ccctaataaa ttagctaagg gcatgatttt ctcagaaagc ttgtccataa aaccgttcat
     6961 ttttattcac ctcaaattta tttgcaatcg attacataca aagtataacg ccaaaaattt
     7021 taaatgtcta gtcattttca taaaaagtat tgatttattt tttagggttc gttatgatta
     7081 cggtatcaag aaaaaagaga ggaagtaaaa aaatgaaaac ctacacattc ccagaaaatt
     7141 tttggtgggg ttctgctgct agcggaccgc aaacagaagg aagagtaagt gatgatggta
     7201 aaggagaaaa tatttgggat cattggtatg aattagagcc tgaaaaattc ttcgatcaag
     7261 taggacctga aaaaacttcg caagtttaca caaaatacaa agaagatgtt cagttaatga
     7321 aagaaactgg ccataacaca tttcgtacat ctatccaatg gagtcatttg attccagaag
     7381 ggacaggtga aatcaatcaa aaagctgttt ctttttatcg agattatttt actgaattgc
     7441 tggataatgg tattgaacct tttgtgaacc tttaccattt cgatatgcca ctaccactgc
     7501 aagaaaaagg cggatggtta tcaagagaaa cggtcgacgc ttatgtttct tatgcagcag
     7561 cgtgctttga actgtttgga gatctggtca aaaaatggtt tactcataat gaaccaatcg
     7621 tacctgtaga aggaggctac ttgtatcaat tccactatcc aaatcatatc aatatgaaag
     7681 aagcggtcca agtaggctat catgagaatc tagcaagcgc aaaggtaatc aaagcctacc
     7741 atgaaggcgg acatgatggt gagatcggaa tcatcttgaa tctgacacca agctatccga
     7801 gagatgaaac gaacccagaa gatgtaaaag cggcgaagat cgctgacgca ttcttcaacc
     7861 gatcattcct tgaccctgca gtgaaaggag agtttccaca agagttgatc gaaattgtca
     7921 aagaattggc tataatgcca gagatccatg aaggagacct agaactcatc cgtgaaaaca
     7981 cgattgacct gctaggaatc aattactatc agcctcgtcg aatcaaagca aaagaaactc
     8041 caatcaatca cgaacatagc cccatgccag atgattattt tgataattac gacatgccca
     8101 ataaaaaaat gaatccatat cgtggatggg aaatttacga aaaaggaatc tatgatattt
     8161 taacgaatac aagagaaaac tatggtaata tcaaatgcta tatttctgaa aatgggatgg
     8221 gggtcgaagg agaagaacga tttgttaaca gcgaaggaat tattgaagat gattaccgga
     8281 tcgagtttgt tacggatcat ttgaaatacg tccatcaagc aatccaagaa ggaaccaatt
     8341 gtgtgggtta ccacatgtgg acatgtatgg acaactggtc ttggacaaat gcttataaga
     8401 atcgctatgg ctttatcgct gttgatttgg cacaagacgg taaacggacg atcaaaaaat
     8461 caggtcgctg gtttaaaacg gtctctgaaa aaaacggatt tgatgcctga tattttttga
     8521 taaaacgttc acaaaatgtt tgttctaaca ctgtaatcgt gctacactaa cagtgaagga
     8581 ggcgtttaca atggtagata cttatcgtaa tattttagta gcaattgatg gttctgaaaa
     8641 ggcagaaaaa gcatttcttg aggcaatcac gattgccaaa cgaaatcagg caacgttgca
     8701 catcctgtat gtcaatgaag taacaggcaa ttactttggt gattttgcat ttgttacaac
     8761 aagccttcag gaagaattgg atgaggttgc ggaaaaccag atgaaagaac atcgaaactt
     8821 ggcaattgaa aaaggcctga cagatatcga gacatatgtt ctttacggtt atccaaagac
     8881 actgattgct aatttccatg aatcgaaaga accaatcgat ctgattgtca tgggggcgac
     8941 aggtctgaat gcggtagaac gtgcactagt aggttccact acttcctatg tagtcaacca
     9001 cgcatcatgt aacgtcatgg ttgtaaaata aataaattaa gggaacagaa agcaaaaaag
     9061 cataacgctt cttttgcttt ctgttccttt tatttttttg taaaaaatta ctttacctga
     9121 tgaagtaatc atgctattct aattacaacg acagatggag taatatggag gaaagaaaat
     9181 gatttcaagt gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa
     9241 aaacgattct tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta
     9301 tgtgatcaaa gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat
     9361 tgtttcgtat cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga
     9421 aatagggcgt aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt
     9481 cgatcaattt ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt
     9541 tatcggagta cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat
     9601 ggaagaccat tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac
     9661 agtaatttcg acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa
     9721 acatcaagct gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa
     9781 ctactggaaa gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag
     9841 cttatctttt gcaagttttc ttttcttttg ttcagcagga tatgtgttca tgggtatttc
     9901 ttgtctgatt ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat
     9961 cacacgttta aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc
    10021 aaaagaaaaa gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg
    10081 attatgtatt ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag
    10141 tatcggtgcc tctatatttt ttgttacggt aggaacgggt gtcttcttga tcatttatgg
    10201 cagtctagtc cgtcattctt atcgtcaatt cactcaaagc gcttattact ggtgaatgta
    10261 ttcagatttg taaaaa
//
LOCUS       DACENJ010000087        10179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.088, whole genome shotgun sequence.
ACCESSION   DACENJ010000087 DACENJ010000000
VERSION     DACENJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.088"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            130..963
                     /locus_tag="IW748_001579"
     CDS             130..963
                     /locus_tag="IW748_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1104494.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            979..1716
                     /locus_tag="IW748_001580"
     CDS             979..1716
                     /locus_tag="IW748_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1104495.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(1871..2839)
                     /locus_tag="IW748_001581"
     CDS             complement(1871..2839)
                     /locus_tag="IW748_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1104496.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            3071..3904
                     /locus_tag="IW748_001582"
     CDS             3071..3904
                     /locus_tag="IW748_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1104497.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            3938..4777
                     /locus_tag="IW748_001583"
     CDS             3938..4777
                     /locus_tag="IW748_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1104498.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            4803..5363
                     /locus_tag="IW748_001584"
     CDS             4803..5363
                     /locus_tag="IW748_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1104499.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            5562..7283
                     /locus_tag="IW748_001585"
     CDS             5562..7283
                     /locus_tag="IW748_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1104500.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            7448..8590
                     /locus_tag="IW748_001586"
     CDS             7448..8590
                     /locus_tag="IW748_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1104501.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            8606..9817
                     /gene="thiI"
                     /locus_tag="IW748_001587"
     CDS             8606..9817
                     /gene="thiI"
                     /locus_tag="IW748_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1104502.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         3479 a         1714 c         2136 g         2850 t
ORIGIN      
        1 agtccattcc ctgtttttta tggtccctgt gctataattc taaataaagg ataaggattg
       61 gaaataatcc ttttatcatt aaaaatatga tgacgaagta gaataaaatt gggagtaggg
      121 gggaaagtca tggatatcca aactgtttta tctgatctga aattaaatgg taaagtgatt
      181 ccagttgttg gtggcgatgt taatcagacc tatcgtataa aaaccgaaca tcgtgcttat
      241 tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg aagcagaagt agacggatta
      301 aaagaactgt cagcattcgt acgtgtaccg gatacgtata tgctaggtga aacaagtgaa
      361 ggcgcttatt tgttaatgga atggatcgag ccgggaaaag gggatcaacg agatcttgct
      421 gcagctttag ctaatctcca tcagcaaaca gcgccacaat ttggttttcg taaagataac
      481 tatttgggta cacttgttca gaaaaattct tttgaagaag attggtggac ttttttcttt
      541 aaagatcggc ttgaatccca gatttctttg gcagaagaaa cgaatcgatg gaatgtacag
      601 cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga aatcggttga accaaaaaag
      661 atcactccac gcctcttgca cggagacctg tggagtggta atgtgttctt tgaccagcaa
      721 gggcatccag tttttgtcga cccagctgtt tcttatggga atcgggaaca agatattgca
      781 atgagccagt tattcggtgg tttccgaccg gaatttttag atgcttatca aactatattt
      841 ccattagaaa agggatggaa agaccgactg ccgatttacc agttatatta tcttttggca
      901 catttgaata tgtttggaga aagttacggt tcccaagtag atcaattact agaaaatttt
      961 taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa agcatgaagt ttcatattat
     1021 gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt taggagtagt tatcaaaacg
     1081 tctgaagatc aaagcgacca gttgggaaga ggcagtgaat tcgtcaatag ctttggactt
     1141 acttgggtca tcacgaatta ttccatggag attaatcgtc tccctagagt cggtgaagta
     1201 atctcagtaa cgactcaagc catggagtac aataagtttt tctgttatcg gaacttctgg
     1261 atccatgatg aagatgggaa agaattagtc aaaatagaat ctgttttcgt attgatggat
     1321 tttgtgaatc gaaaaatgag cagtgttaat gaagagatca ttgctccttt tgagagtgag
     1381 aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga ttgattcggg cttcatgttg
     1441 ccatatcgtg ttcgttttta tgatatcgat agtaatcagc atgttaataa tgcgatgtat
     1501 tttaattgga tcattgacgt attaggatat gactttttga ctacgcatgt tccagaaaaa
     1561 gtaattattc gatttgataa agaagtagag tacgggaatg agatcgaaag ccattttgaa
     1621 caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa tccgaatgca agaacagctg
     1681 tattgtgaag caaatattat atggaaaaat aaataaaaaa gaggttgtga cagaaggggg
     1741 caacttcaag aaataagaag aaatttacga aaattgtttc tcaaattttt gtaaattttc
     1801 gacttagttt tcgaagaagc tatttttgtc acagcctctc tttttaagtt tagctattag
     1861 ataatgatgt ttagttttgg ataaacgaaa tcaattcttc acccgacaaa ccatagttta
     1921 cagcaatcaa tagttttaaa cgtgcttttt gactattaat actgttacag aaaataatcc
     1981 ccattttttc tagttctttt cccccgcctt cataatcata aacaggctct gcgattccat
     2041 tgaaacaacg agaaacaagt actacaggga ttttttggac aagaagcttt tccaaagcag
     2101 ctaaggcttg tggagggaga ttccccgcac ctaaagcttc gattaccaaa ccgtctactt
     2161 tcgtatgggc gatggcttcc aataagtccc cttgcatccc agcatatgct ttaacgatcg
     2221 ggatagtccc atctacagcg gaaatgtcca gtcgctttgt ttctaaaagt tcctgcaaga
     2281 ataaaattcg attcttagtt actaagccga tgggacccaa tgtaggtgtt cggaaagtag
     2341 caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga atggatctca tcgttcatga
     2401 ccaccaaaac ccccttatct agcgcttctt cacaacttgc gacacgaatc gcactttcaa
     2461 aattataaag gccgtcactt ccaagttcat tacttgaacg cattgcccca gtcaaaacaa
     2521 ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc agtttcttct aatgtatctg
     2581 taccgtgtgt gatcaccact ccggatatac ctgatgcata agcggacaag atacgttctt
     2641 ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg aagattgaag atatcttcaa
     2701 ctaataattc tacatgcttg gggattgtga tttctgcatc taataatggg tttgccacat
     2761 caggactaac accgcccgtg agcatgtttt ctttcatggc gatcgttccg ccggtgtgta
     2821 gtacaagtat ttttttcatt ttacagactt ccttacgaat aattttgcat agccgttaaa
     2881 tcttattgta tactatataa aagagttttg ctatcagtga agaataacga atagcctaac
     2941 ttatcataaa aaagctgacc ttattaaaat ggaaaagact caaccttgac ctcctaatac
     3001 aatggagtat ctggttgagt ttcttttaca agaaagcagc caaggaaatc aatcacgaag
     3061 gtggccggaa atgatcagaa tcggattaac atcgtttaaa gaacatgaca aattaactgg
     3121 taaaaagaat aacactcttt atgaatatgc agggtttcta cccttagtgg aattagatac
     3181 tgcctattat ggaattcccc gaaagtcctc agttgagaat tggataagtg aagtacctga
     3241 atcatttcgc tttgtagtca aagtctatca aggaatcagc ggtcaaggag actggaagcc
     3301 ttattatcag accgaagaag agatgattgc gcatttttta gatgttatgt cgccaatggt
     3361 agatgctgga aagatgtttt gctttttagt ccaattcccc tcatcattca aatgtacgaa
     3421 agaaaatgtc cagtacttgc gcaaacttag aaagtggttt gagggttatc ctgtttctat
     3481 agaattgcgg gattattctt ggtacagcga tgaatttctt gaacgtacaa gaaactttat
     3541 gtctagagcc gctttttctt tagccatcat cgatgagcca cagttgctga atacaacgat
     3601 tccttttgat ccatttgtca ccaatccgaa ctttgctctt tttcgattcc atggtaggaa
     3661 tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa aaaagaacgt tatatcgtta
     3721 ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa gtagttgatt ctaagacaaa
     3781 agaggtaggt gtgattttta ataataattc tggtggtgat gcggctgaaa atgcattagc
     3841 actgcaaaaa atattaaata tcaagccaga tggactcaat ccaacccaaa tggatctgtt
     3901 ttaagggctt attttattca agaaaggggc gaagtctatg atcaaagcta ttttttttga
     3961 tattgacgga acattggtca ccaatcgttc aaaagcgttg gaatcaacaa aacaagcaat
     4021 tgaatatgct cggaaaaacg gcattttatg cggtgtagca acggggcgca gcccagtaaa
     4081 gatcaaagaa atcattgatg aattggaatt agatatgtat gtcgtgtata atggtcaact
     4141 ggtttttact gcagatcgca cgattatcga ccaccctttt gaacaaaaag tattggaaca
     4201 tattgttgaa tttgcagatg agaaccatcg acaaatcgtt tttggtgcga gaaatcggtt
     4261 agatggatca acgacgatgc tattaggaca atcgattttt atcaagcgac tagtcagctt
     4321 tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc ctccaggttt ttagccctca
     4381 ccgacaaaag gatcgttatg aaacgttaga gatattaaaa gaaccaattt atcaatgtat
     4441 attgctcagt ccggagtcag aacagaaaaa gttagaacat cgctttcccg agtgtacgtt
     4501 tcagcgttca aatacgtata ctgtagatat catcccaaaa ggtggttcaa aattactagg
     4561 catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa gaaattatgg cattcggcga
     4621 tcattacaat gatatcgaga tgctgaaagg tgtaggaatt ggagtggcca tggggaatgc
     4681 tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa tcaaatgaag aagatgggat
     4741 atacgaagga ctgaaacact tcggtctgat ttattagtca atgaaagaag gaaaaaatca
     4801 gtatggaagc agagacacct tttcaaaaag tagaaacatt ccacaaaact tttgatccaa
     4861 gaagaccaga gatacccacg gcttttacag aaaatgaagc tcatacacgt gctgcgttca
     4921 aaatagaaga acttgttgaa tttgttgcag ctacttcacc taataaagaa caactgcatg
     4981 aagctgttat gctgctgcac gaagcattag ataaagcaga agaaaaaata cttggtaaaa
     5041 cacagtgggg aagtgatccg ctggttgaac aagtagatgc actgacagat atcctttact
     5101 ttacttatgg ttctttttca ttactaggaa tagtgcctga tcgaatattc gaaatcgtcc
     5161 atcaggcaaa tatggggaaa ttatttccag atggaaaacc tcattatgac cctgtaacaa
     5221 ataaagtgtt gaaacctgaa aactggcaga tggaatatgc tccagaacct aaaatcgcgg
     5281 cagaaatcca acgtcagatt caagaagcga ttgctaagaa gaaatccgct gatataggca
     5341 tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt ttgttatctt tatacgatgg
     5401 aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa aatagtatgc tataatctag
     5461 aatagatgtc ttttgcacga gagacgtatt atttggattt atgccaagca gaacgccatc
     5521 tgttgcataa aagatgcata ctgaaaacgg aggaaccata aatgggaaat aatgttatca
     5581 tcggaatcgt cattgcaatc attatagtcg ccattgtcgt ctttttggtt agttacttga
     5641 tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa acgcaaggaa gaattatttg
     5701 acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat gcatctggtt gggcaaagcc
     5761 aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat atctacttcc tcgttcgctg
     5821 aactagaaag tcaaattttc gaagtggaaa acctcaatga aagttttcgg ttcttaaaag
     5881 gaaaaaaagc agttgttgaa gcagaagcta caatggacca aatggagaaa caagtgacgg
     5941 aaatccgtga aggtctaaag gaattgcggg aaacggaaga acgcaactca ctagctgttc
     6001 agcaggcact tgacgtctat gaagaactga aagaaaaagt cagagaagac gagcatgaat
     6061 acggaccagc tctttttgaa ttgcaaaaac agatcaaagt gatcgaaaaa gaatttacgc
     6121 agtttgtgac tttaaacact tctggagacc ctgttgaagc ccgcgaggtt ttggataaag
     6181 cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa aattccaccg ctgtatgagg
     6241 agctccatac cactttccct gaacaacttg aagaaatcag tgatacttac gagaaattga
     6301 tggaagaaca atatgtattc ccagaagaag atcttgctga agatatcgca aaagtaagtc
     6361 gaagaattga aaattcatta gccaatcttg aaaaaacaga agttgaaacg gtcgaatttg
     6421 aaaaccgtga aacagctgat ttgattgatt cgttatatga tatcttagaa cgggaaatgg
     6481 aagctcagag atatgtaaaa actaaccaat caacgattgc agagtatatc aaacatacaa
     6541 caaagaataa ccgtcaatta ttgattgagc tggatcacac atctcaaagc tatacattga
     6601 accacaatga aattggccgt gtacgaggtt tccaaaccga agtggaagaa atggaacgtc
     6661 aaaatgaaca gatgattcca cagatccgtc aacacgaaat tccttattca gaagtgcgca
     6721 cgttctataa aacagtcttc aaagtcttag aagatatcga aaagcaacaa gtagagatcg
     6781 acgactcatt acatgaattg agaaaaggcg aaaaagaagc gcaagaaaag atcgatacat
     6841 tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa acaacgtttg cctggcttgc
     6901 cgaatgatta cctagaattc ttttttgttg ctactgaccg tattgaagaa ctaagtgttg
     6961 tcttgaataa aattcgagta aacatggaag aggtcaatcg tttagttgct ctatgtgaag
     7021 aagatctaga attattagat aagaagacac atgatttagt agatgcagca gcattaacgg
     7081 agcaaatgct ccaatatgca aatcgttatc gtcatacaca cgaagaagtg agagaagcaa
     7141 ttgataaaag ctactattta ttcaataaag aatatcacta tcaagaagca ttagacgaaa
     7201 tcggtacagc attagagcga gtagaacctg gtgctttcaa acgcatcgaa gatttttatt
     7261 ttaatcatcc agacttagta taaagcaaaa aagttagtaa accagcaaga gcaagacgga
     7321 ttattgtctt gttcttgtct ttttttgtat atttgaaaat tgaaacaagt gaaaatttca
     7381 cctgctcttg aaagcacctt ggacaaccgt tataatagaa aagaatttca aaaaagaggg
     7441 gtttcttatg atatattttg ataacagtgc aacaacccca atctttccgc aaagcttaga
     7501 tgcatatgta aaaaccagtc agcggattat tgggaatcca tcaagcttgc atgatttagg
     7561 caatcaagct aaccggttgt tgcagcaagc aagaaagcag atcgctgatt tgatccatgt
     7621 agaaccagaa gaaatctatt ttacttcagg cggaacagaa ggagataact gggtattaaa
     7681 aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg atcatttcag gagtcgaaca
     7741 tccggccgtc agcgagacag cagaacaatt aaaagaatta ggaatcgaag taagtatcgc
     7801 cccagtggat aaacgcggat ttgtacgtgt cgacgaattg aaagaattaa taagaaaaga
     7861 gacagtatta gtctctgtta tggctgtgaa taatgaagta ggatcgattc agcccatcca
     7921 ggaaatcagt gatcttttgg atgattttcc taaaattcat ttccatgtag atgctgttca
     7981 ggcgattgga aaagtaccat tctcgcaatg gttgacagaa agagttgatt ttgcaacatt
     8041 ttcagctcat aaattccatg gcccacgggg aactggtttc atttattgga aaaagggtcg
     8101 tcgtttagca ccattactga caggtggagg gcaagaaaga aatcagcgaa gtgggacaga
     8161 aaatgttcca ggtattgtgg ctatggttaa agctttacgt ttatcattag agataaagga
     8221 acaaaaacct gagcagacac aacgcttaaa agtagctcta atcaatgcgt tgaagacgta
     8281 tgagaaagtg acgatttttt cagaaggaac tgactatgca ccgcatattt tatgttttgc
     8341 tctaaaagga attcgcggcg aagttctcgt ccatgcctta gaagaaaaac aaatatttgt
     8401 ttctactact agtgcctgct cctcacgtaa acatatggca agcagtacat tgcacgcaat
     8461 gcatgtacca aatgatctgg ctacatctgc tgttcgaatc agcttagatg aaaacaattc
     8521 gcttgcagaa gtcgagcaat ttatgattat tttcaagcag ctttataaga aattttctgc
     8581 aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa attatggtcc gttatgggga
     8641 actttcgaca aaaggaaaga atcgaaaaag ctttatcatg caactagcac aaaatgtcag
     8701 acaagctttg aaagactttc cagaaatcaa aatccatgct gatcgggacc ggatgcatct
     8761 tttattgaat ggcgcagacg ggcagctagt tattccaaaa ctagcaaaaa tatttgggat
     8821 ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc gaagttttga aacaagctgt
     8881 tcaagacatc atgaaagaaa tttatactgg gaaagaaaca tttaaaatta tcgctaaaag
     8941 aagcgatcac caatttgaat tgacaagtaa tgaattgaat caaacattag gtaatgctgt
     9001 ttttgatatt ttcccgcata tccaagttca aatgcgtcaa ccagatattc cattacgtgt
     9061 agagattcgg cgtgatggcg cttatctatc gtatgaaacg atcaagggag ctggaggatt
     9121 gcccgtagga acaagcggaa aaggaatgct tatgctttct ggggggattg attctcctgt
     9181 agcagggtac tttgccatga agcggggagt ggaaattgaa gctgttcatt ttgctagtcc
     9241 gccgtataca agtgaacaag cactgcaaaa agcaaaagat ctaacagcca aactagcacc
     9301 ttatgtggga acgatccatt ttatcgaagt gccatttacc gagattcaag aagaaatcaa
     9361 aaagtcagtg ccacaaggat attggatgac aattactcgt cgaatgatgt tgcgtttgac
     9421 cgatgaaatt cgccgtattc gtcatggttt agtcattttg aatggtgaat cattaggtca
     9481 agtggcttca caaactttgc aaagtatggt ggctatcaat gaagtaacga atacgccaat
     9541 cattcggcct gtagcgacaa tggataaact tgaaattatt gaagtagcag aacagatcga
     9601 tacatttaat ctttctatcc agccttttga agattgctgt acgatttttg ctcctccaca
     9661 gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat gaagagcgtt tagcaataga
     9721 tgaaatgatc caacgagcac tagctggctt gaagatggaa gagatacaag cagaatcgtc
     9781 taatgaaaaa actgaagaat tttcagaatt gctataagag gttatgaaaa tagtgttttg
     9841 accagagtat tagagaaaca aaacgaaaaa atagctccat gtattttacc gtttttgaac
     9901 taatgtcgaa gattagctga ttgaatacca tttatcaaga agttatggaa aagccggttc
     9961 ttttttacta actgaaaaat aaaattttat ttagagatca agctacagtt gataaatgat
    10021 ataaaaaaga gaatttccca tgaaatagcg aggttgtgac agaagtgggc agcttcaaga
    10081 aataagaagg aatttacgaa aattgctttt caaatttttg tgaaatttcg gcttattttt
    10141 cgaagaagtt acttctggaa caccgtgtat cagaggttg
//
LOCUS       DACENJ010000088        10156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.089, whole genome shotgun sequence.
ACCESSION   DACENJ010000088 DACENJ010000000
VERSION     DACENJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.089"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1110)
                     /locus_tag="IW748_001588"
     CDS             complement(<1..1110)
                     /locus_tag="IW748_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1104503.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKK"
     gene            1171..1911
                     /locus_tag="IW748_001589"
     CDS             1171..1911
                     /locus_tag="IW748_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1104504.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(1997..2755)
                     /locus_tag="IW748_001590"
     CDS             complement(1997..2755)
                     /locus_tag="IW748_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1104505.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(2752..3780)
                     /locus_tag="IW748_001591"
                     /pseudo
     CDS             complement(2752..3780)
                     /locus_tag="IW748_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(3800..6967)
                     /locus_tag="IW748_001592"
     CDS             complement(3800..6967)
                     /locus_tag="IW748_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104506.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(7191..8075)
                     /locus_tag="IW748_001593"
     CDS             complement(7191..8075)
                     /locus_tag="IW748_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1104507.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(8170..8607)
                     /locus_tag="IW748_001594"
     CDS             complement(8170..8607)
                     /locus_tag="IW748_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104508.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(8824..9735)
                     /locus_tag="IW748_001595"
     CDS             complement(8824..9735)
                     /locus_tag="IW748_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1104509.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2910 a         2085 c         1704 g         3457 t
ORIGIN      
        1 ttttttagag gtttttcctc ttttgaccat ctccgatagc gtcaaattcg ttggatttgc
       61 cttgttcgct atactaaaac gtgatccctc tttttcttcc agcagattga tcgtaatcgg
      121 actctgatcg gctacacgat gatcgaatgc gttagaaata aaaatagata aacagcgaat
      181 aaaaacaacc agatcaatcg ggattgtttt cacttcgaca ggaataacta cttggatcgg
      241 gattttctcc gattctgctt tttctagaaa ttttaaaaag acacccctga cagctgggga
      301 tacaatattt gttagctgtt gtatttgcgg tttttcaaaa atagctgcag aatgatcttc
      361 taatatttct aaaaattcca acgtttcttt tttattttcc tgaagagtaa tttttaatga
      421 aaacagtaga cttttataat catgtttgaa ttcctctaga tgatggtaat attcttcatt
      481 tatttgatac agttgaagcg attcctgatg ttgtctttct tttacttgga aactgatcaa
      541 cataatggaa aaaaacaaag aaaaaaaact gatgactatc gacaaaagag atagaagcag
      601 ataattagat gagcggaagt gaacacttcc caataccaag taaatcacgc cccagcttaa
      661 taaaatacaa atacctccaa aatagtgttc agttggataa gtaaaaagca agttgagaaa
      721 tctcgtctgt ttgtctatac tttttactaa tagatagaaa ataaatgcga taagtagtgc
      781 aaaaaaactt gctgttccaa gatgtctctt tcctaaataa tccatcagat caaaaaccag
      841 aaaaaagctg agtccagtga tagcaaaaaa aagattactg atcaaacagc tgataaacag
      901 gtttttcgta gtaaaataaa aaagaaacat caataataag ccaactaggt aaggaaagct
      961 cactttaggg ataggaagta aaagtgtcac caccatcaaa gctatacaac caaagctgtt
     1021 ttttactagc agttgtttcg aaaagaataa atagatacct aaatctaaaa tcaaagtaaa
     1081 ggtcaaataa agtgtcactg ttgcgaccat cttattcctc cattgttttg tttcaaaatg
     1141 tacacatatt tttttaatag gagcctgcat atgaaaaagt attttatcat agaagataac
     1201 cagtttcaca gcgaatggat catcagagaa ttggtcaatc atcatatcga gaatatcgtg
     1261 cagctcactt ctatctctca aatcaatcag ttgatccagt tgggcactat tgataatcac
     1321 gatgttttct ttatcgatat cgacttgaag caatcactca caggtattga tgtcgccgaa
     1381 caaatacgta aaaaaaatgc acatagtaat attgtttttt tcaccgctta tacgaatttt
     1441 gcagtagaga tcatcaatcg gaaaatcctt ccgattggat atatcagaaa agacaggaat
     1501 ttatctgaac aaattcgtca agtgctagaa cagatcaatt ggaaagaaca gcagatcgta
     1561 aatgtcgata aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta tcctcgggaa
     1621 attttatata ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa aacaactaca
     1681 gaagataaat tagtgaatac ttctttaaaa caattgaaga cagagttaga aaagtatgct
     1741 tactttttac ttttaaaatc ttactgtatc aacttatcgc aattgcagaa aattgattct
     1801 gctcattatg tgttagaatt tttcaacggt gactccttac tcgttggtcg aaagatcttt
     1861 gaaaagacaa aggaacgttt ccatgacttc caaaaaactg cttcgagttg atgaataaga
     1921 gctgagacgt aagcatagca gatttcaggg agtgggcctt gaatcggctc cactccctga
     1981 aatcgttgat atgattttac gcttcctctt ctgtaggagc atctgttaac gaccatgtga
     2041 atgttgaaac gtagttgtct acagcataat tggatacagg aacaaataat tccactgatt
     2101 gtctcccttg ttccacattc tctcctaatg aataaatcca tgtgccaatc ccttcatttg
     2161 ctcctgcagt tactagatct actgcctgat ctgctgggat ttcgattcct tcagcgccag
     2221 ttccagaaac ggtagatggc attccactgc tattagttgc gctatttcga atcacggcgt
     2281 ttttgaattt cacctttgaa ccagttagtt tattcgtatc agatgttgta ccaataaatt
     2341 ctgttcgttt gactgataat ttccaaccgg cataatttcc gcttttatcc gttacttgta
     2401 cgaaattctg tacttgtgtt tgtgctccag tactgtccgt tccgctttgt aatgcagcat
     2461 atactgtttt tgctgtacct aacttgattg ttccaaaatc aagatctgga gcttggtcaa
     2521 tcgtcagtaa tccggatgtt ccctgatttg gttcgtcagg tgtaaaagga gtatcaggat
     2581 catttggatc aacaggagga actggaacaa cgtcttcagg atttgcagca tctggtttgg
     2641 tcacagaaat cgttccttct gtagtggctg agctcttatt ctcagcaaaa acactttgtg
     2701 tacataataa tagacttcca gctagaacgg tacttaatgc aattgttttt ttcatttctt
     2761 tttccttctt tcttttaaaa taaataatcc agcaaataag ccaataagaa tgactccaac
     2821 aagtatcagc catcgccagt caaatgtttc tggctgattt tttaaatcaa tatccttttc
     2881 attcaattgg tccgctgcat catcagttat ttcaaaatcg gtatggagat tccagcgttt
     2941 ttctttccaa gcacccttca caaccgcttg ataatgtccc ggagctaatt tttttccttc
     3001 taatgggaca agtaaatcaa aatttgtatt gggtgctaca tctatttttt ctttttttat
     3061 tgaaaggtaa ggctcttttg tttctttttt gtagatttca accgatgtag tcaagccgaa
     3121 catataagaa gcatttggat tttgaagatt cgccttaatc acatttcttc catcgctttg
     3181 atccgcataa gcttttttta atttcaaatc tggggaagac tctttttcgc tatttcaaat
     3241 caagattgct ttcacttgag aaaactgatt gacgacacca actgtacttt tgttagaaac
     3301 gttctctttt ttttcttgaa acgtgatccc tcctgcgacc acaccagcca gctctttttc
     3361 tggcgctttc agtgaaagtg tctcagtgac cgcactttga ggaggaacag ttatttctcc
     3421 cactgttttt gctaagtttt tcatatcata acttaatgtt tcatcaaatt tactttttga
     3481 ttctccatat tcgatcacac cgtttgaatt cgtcgtagcg gagtggatca atgtctcaac
     3541 tgttactgat ttatctgttc ggttaaagac cgtgtagggg atttcctgtg ttttcccagg
     3601 ctccatcctt acatttatat atgttttgtt cttatcaatc tgattagccg gtaattcaaa
     3661 atctaccgca aaattgaatt cggaggcatg gacaggtgtc tgccaaagga tgaatccaag
     3721 gagaaggatc ataagtaaag agcagttgtt tcgatgactt ttattccata ttcgattcat
     3781 caatcatcct ccttgatctt tattctgtcg gcgcatctgc taatgtccat gttatctctg
     3841 ctgagtaatt accagctatc tgattttctt tggttaagac aagtttaaac gcccgaccgt
     3901 tatttaaata ttctgaataa tctgcttgca cattcgttga attttgtcct ctgacaattt
     3961 ccagttcttc ttcattcact aattgatact cctctccttt cttgaaatat aggacatcct
     4021 gcatagaaaa tcctgcatca tttgtgaaag gctgagataa ttttatcgtt aagatccacg
     4081 gatttttcgt ttctcgctga tcataaatcc ataatttttc ctgtatatcc atttggccaa
     4141 atgcttcttg tgtcaaacca gttgctttga tctgaccaaa atcgagtttg gaaggggcaa
     4201 gaaatgagag accaccttct actacgatct tcgtagcagg tgcaaaaggc ggggcatcat
     4261 gataaacaac tggcgtatca cttggattgt gattcccaat ttcatcgttg gtacttggct
     4321 tgttctgcac acccttagca gtagcttctc ctgcttggtc gtttaataca atttctaata
     4381 catctcctgc aaaaagttga ttttctggga tagaaagaac aaattcccct gtatcatcaa
     4441 tagttgtttc tgacaaaaca gcttgatttt ttttcaatga aaccaaggct cctgcttcac
     4501 ctgtacctgt gatcgtgcga gaagctgcat tgatcaatgt ggtacttaaa ttagcaggtt
     4561 caggaggaat cacatcgact gtcgttttgg cagagtccag ttctgtcact tcttggccac
     4621 ctcttacttt tttagcagca acaacttgca cttgatcctc cgtttttaat ctctcatcat
     4681 tgggtacttg aacttcgaac aagccgcctt gttcgccttc tccccatggg ttatatcctg
     4741 ccccttcatt ttcatctgtt tgtccttgcg tgagagcagt aatttctaca gatgatttcg
     4801 tcgtatctgc gtaagtaata cgtaaagtca cttctacttc gcctgtccaa gcatctcttg
     4861 gctttgcctc gacaccttcg ggaacaacga catgcccata gattttttta tctgcattcg
     4921 ttggcactct taaggattca atcatcggga gctgattatt ggcactgatt cgattataat
     4981 ttttcattcc ggcacctgtc tgagcaaatt catttgttaa acctgcacta gggcttgata
     5041 cgaaattgcc taagtcggtc ccattgatcg ataagtctgt catatcagaa aaataatccg
     5101 ggtctttttc aaagttatcg aatgttgcgc ctgtttttcg ccataaagcg atttctgaat
     5161 gggcgatctt taaagaagaa tctccatttc cttggaatac ccagccgcct atgccattgg
     5221 tctcgcttcc aatccgtttg ttttggaaat caaaataggt aggagattct aagtccacat
     5281 tcaacacatc cccactgatg atgccggtct tatcttcccc tgttttcccg accatgacta
     5341 aataagtttt ttcacctgcc gtaaaattta aatcaatatc tccagttgta tctacagttg
     5401 ctccggaatc tgcgcgtata tcgatatttg atttttctcc agtgactgta aaatcagctg
     5461 aacctggact ttctagaaaa ccagaagcgt cataaaattg gatcgcttga tttccttgat
     5521 tgagtctgtt cacgtaagat ttgccatcac ctgtatttct taccctcaat gagcctccat
     5581 tggttactgt cagcttttgc gtaccattct caaaacgaac tgcgctggaa cgcccactct
     5641 gcttatcgat gtcgataact gatccggctc cttctacgat caaagaaagg ccttttccta
     5701 caaaacgcat cccattgttc ccgggttgat cgtcatctcc agccattttg accgagattt
     5761 gtccaccact tgatacatgg atttgactag aatctccttg caagcgaaat ccgcccgtag
     5821 ttgctgtatt gatggtgatt ttcccaccat tcgatacagt gactttcgat cctgaacctc
     5881 tcatttggat gattccgcga ttgttgtcat tggtaatatt gatctttgta tttaccgaaa
     5941 attcgcttcc cgaaccactg acttcaaaca tgacattacc aggtagcgta tcgactgcct
     6001 ctccttcatc gctaacgcca tctgcccgat cataatcaag agagaccttt gaaccgctta
     6061 agatatggat tcctggcgct gtttttccac taccagtctc atctcctgaa tctagaaaac
     6121 cgtagaattt ccctagatac ggtccataaa gttgactacc attgatcaca ttcaattgat
     6181 agcttctaca aaaataggct ttacccggag cggcattcag tccttccttc actggttgga
     6241 catccaacac accggaaaca ccatctaatg tgacagttgc acgaggagcg taaatcagtc
     6301 ctaatttgga cccatcatac aaatcaagtg tcccggtaaa ttttatttct cccgtaaaat
     6361 gatatctgct tccccaaaaa tcggtcatat aagcaatgat aatggcattt ccatcgatat
     6421 ttggtgtcaa tccacctgga tgtgcagctg ttcctttaaa attctggaac tcgatcgact
     6481 gaggtatctc attgatataa atatggtgtg tgccgaaatt gtgagcgaat ccaccaaaat
     6541 caataacgat atctcgtttg atctctaatt ccctgtctaa agtcaaaggc tgaatcagtc
     6601 ggatcttagt tactgattcg tctgcgagaa gctgcgccag ttcttctgtt gtggcagctt
     6661 cgctgtaccc atccgctgaa acagctcttg ccagtgccgc ttcactgcta tcagacgttt
     6721 gttcagctac ttctgaggtc tcacttgtct tattttcttc ttgcctttta ttagtagaag
     6781 cctcactttg agattctgta gtttcagaag taatcgattc ttctgaaaaa gtagtcttgt
     6841 caaaactttg attctctgat tgcgttgatt cgtttgtttt ctctacatcg gcaaaaacgg
     6901 atgtcaccat caagagaggg aagacaaaca aaatcgacgc aatcagcttt tttacacatt
     6961 gattcattct ccaccatcct ctctgacgtt ttgacaattt tattttatcg ttttaatcta
     7021 tttccaattg tactttcata tttaaaatat gtatttttat ggaaaaattt ttctattagg
     7081 tgtaaatttc ttttttgtac aaaaaaaagg ctgtgacaaa agtcttgtca cagtccctta
     7141 tcccgaacaa gcggtgttct cccgccaact cctcggtcac aacctccatt ttaacaataa
     7201 acctgtaaat gtgagggcaa tactttgatt gtcacaggta atttatcccc ttcatctcca
     7261 tcaacattcg tcgtcaattc ttcctcatca gctaatgaca caactaattg ctcacaagta
     7321 aggtaggcta gatttttcgt agattcatct actcctttta ataggtctgg caatgtttta
     7381 agcgtatcga tcaaagaaga atcttttaaa taaaccaaat ggagcttacc atcatccact
     7441 tttgcatttg gaagtaatgt ttcaaaaccg ccgactgagt tagttagtcc tatcagcaag
     7501 gtactgctct caatttcttc cttctttcca tcaacttcta cgtgaaacga gtaggattgt
     7561 gtggaagcta actgtttgat tcctgacatg aaataagcta gttttccaaa tttcgtcttc
     7621 ttctccgcat ctacatcatt gattgattca gggatagagc caattgcgac gacattcata
     7681 aaatagtcgt catttatttt tccaatatcc aatggcttga ctgattcaat cgaaaaatga
     7741 ttgatcgctt cctcaggttc tagcggtatc cctaatgctc gagccaagtc attcaccgta
     7801 cccaatggga aaaatccgaa atttggccga tgttcttgtt cagcgattcc actgattcct
     7861 tcgttgactg ttccatctcc gcccatgaca aacacactat gatacccttc cgtagccgct
     7921 tcgcgagtaa agttttttgc atcccctgct ttttttgtat gcaagacgac cacttcatca
     7981 aacaccgatt ccaattttgc tatcgctaat tgctcaaatt cctttgcttg ttcaccgcct
     8041 gaactggggt ttaccactaa taaaactttt ttcatcactt tctttccctt ctgattgatt
     8101 tctttaatat aacgatagca taaagaaaaa aattaggctg tgatgaagta ctgttcatga
     8161 aacattcatt tattttttga caagataaaa catccatact atgaaactga ggaaaattaa
     8221 aacaatgata taggaaataa aagaaaatga taggcattgt atacctaata aagtaaaaat
     8281 cttaggtggc agataaaaac acaaaaatag caagagaatg taatagcgtt tcttccactt
     8341 tttgttatca gaccttatag cgatcagagg aataccaaat gcataactga aaaactcttg
     8401 atgtactgtt aatatgtcac ggccttgaat aataaatgcg acaatccccg ctataattag
     8461 tgtgcctaac accaaaacgg aaaattgatg aatatatttt atatacaaat gatccattgt
     8521 acttcctgcc tttttatttg tgagaaaaac aaatccgtat acgtgaataa cttctagaaa
     8581 taaaagaaat ttgcaaaacc actccattta tctacttcta cgtgcatttt ttgatattgt
     8641 tttctttatt taagattata cagaaaaata tctagggtcg ctggattcta agtaaaaaaa
     8701 atttttaaaa taaaaagaac taaatcaagt agtaattaaa ctactgttga tttagttcta
     8761 ataaaaagat ttagatgaac agttttattg atttaaatag ttcatatttt ttatttatac
     8821 cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa cattttcaaa
     8881 gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta ctttattctg
     8941 gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt
     9001 atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat attccgtagt
     9061 ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt tcaacttaaa
     9121 ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc
     9181 gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag agaaatcgtc
     9241 tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta cttggataat
     9301 ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt catcggtcaa
     9361 tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac tgtctttgaa
     9421 tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac ccaattgttt
     9481 ctttgtttga tcatattgag aatcgatttc tttggttgat acgtcatctc catatttatc
     9541 ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt gcattttttt
     9601 caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa cggctgtttt
     9661 ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg caagtgctaa
     9721 aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt gctaaaaaca
     9781 aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga tacgtcctga
     9841 atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt caagtagcac
     9901 atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct tgcgttccgt
     9961 atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt cttccgctgc
    10021 tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt tgttgttttt
    10081 attatgatat atttttatac aactaaaaat gaatatatta aaactaccaa aaataacttt
    10141 taacgctgtt tcatta
//
LOCUS       DACENJ010000089        10022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.090, whole genome shotgun sequence.
ACCESSION   DACENJ010000089 DACENJ010000000
VERSION     DACENJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.090"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..1363
                     /locus_tag="IW748_001596"
     CDS             68..1363
                     /locus_tag="IW748_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1104510.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            1353..2813
                     /locus_tag="IW748_001597"
     CDS             1353..2813
                     /locus_tag="IW748_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1104511.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            2949..4445
                     /gene="glpK"
                     /locus_tag="IW748_001598"
     CDS             2949..4445
                     /gene="glpK"
                     /locus_tag="IW748_001598"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1104512.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            4459..6282
                     /gene="glpO"
                     /locus_tag="IW748_001599"
     CDS             4459..6282
                     /gene="glpO"
                     /locus_tag="IW748_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1104513.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            6283..6999
                     /locus_tag="IW748_001600"
     CDS             6283..6999
                     /locus_tag="IW748_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1104514.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            7305..9234
                     /locus_tag="IW748_001601"
                     /pseudo
     CDS             7305..9234
                     /locus_tag="IW748_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            9360..9914
                     /locus_tag="IW748_001602"
     CDS             9360..9914
                     /locus_tag="IW748_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1104515.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         3369 a         1674 c         2279 g         2700 t
ORIGIN      
        1 ttatgaatac tgtgttttct gattgagaag ttagcagtag aacaccttat aagaaagagg
       61 aatgcagatg aacattgtaa ttgtcggcgg gtcgtttgca gggattcatg cggctattta
      121 tcttcgccaa ttgatgccaa caagtgaaat atatttgatt gaaaagcaat cgaagttagg
      181 atggattccc agcgggttca atttgatatt aaaaggaaag gttaccagtg aaaaacaact
      241 ttcttggatc acgaaagaag aactgactga ccgctatcga atccatgttt acacagaaaa
      301 gacagtaata ggattgaaag aaaaaaaagt cgttttagca ggaggaaaaa aattggattt
      361 tgatcgtttg attctagcga ctggttcaaa tcaaaagttt cgaaatattt ctgcagaatc
      421 aacgcttatt cacccagtta aaaagataca aaacgtaacg gctcttcagc aaaagatcat
      481 gaatgcaaag aaaattgcaa ttattggtgc aggacaagca ggaatcgaaa tagctgaagg
      541 gttggcatca agaaaaaaac aaatccatct ttacgaaagc cgaaagtcaa ttctttttcg
      601 ttatctagat cctgaaatga cagaaccgtt agtaaaggaa atgaaaaatc aagggctttc
      661 acttttttta gaagaacaag ttgtgtcttt gaccgaaaat gaaaccgcta ttgtagagac
      721 ggaaaaaaga aaggagaact atgatcttgt tttgctggct aatcattctc gtcccgacaa
      781 tcagatgtgg gaagaacaat taacattgaa tgatgatgga acgatctggg tagatgacta
      841 cttgcaaacg tctcaaaaag acgtttatgc gattggcgat gcgatccaag taacttttcg
      901 tccaacaaat gaaaaaatgt atgtttctct agtaaataac gctgtccgta cagcacgaaa
      961 tgtcagcaag acaattagtg gctcacagac aaaggatcag ggaacctatc gtccagtcgg
     1021 caatcagtgg tttggctatt tcttaggaag cgtaggtttg acagaagaag agagcatttt
     1081 ttatccccaa aaaattgaaa caggatacgt agactacaag cttacagcaa taaatcaaag
     1141 caaagttaaa ctcaaatttc tatttgattc tgaaaaaaag attcttggag cacagctcca
     1201 aagcaaaaaa gagatgttcc accttcttga ccaactgaca attgcagtag aagaaggatg
     1261 gacgtttgaa cagttagaag aacatgagct atttttccag ccagaatacc gtgtgccaga
     1321 tatggtgtgg tcaaaggtgg cgaatactgc ctatgaagat tgaagcatta ttagaaagaa
     1381 aagagcagtt acagatcctg attttgcgaa accttgttct gcaaggggga acagcctcta
     1441 tcaatggttt gagagagcat gtccaactgt caaaagcctc gtttgatcaa tatttggaag
     1501 atattgaatt gattggccga atgatggaaa aaaaagtaga agtacagcga aatgagtatc
     1561 aggcgctgct tgtgttggac gaagatgtaa gtttggaaaa aattctattg tttttattgc
     1621 aggaaagctt gaagtttaag atgctcgtct atttattaga acatcagcaa gtatccatcg
     1681 ttcgattagc gacagcgttt aatatcagtg agtcttctgt ttttcgaaag atcaaagaat
     1741 tgaaccagct cttagaggaa tttggattac aaatcaaaaa cggtcaatta tacggagaag
     1801 agctacagat acgatatttt tattatgaac tattccaata tatcccggaa gatcagcgac
     1861 cgcttttttt gcaaaataca ccagaaaaaa gaccgttcat tctaggattg gaccgtgtat
     1921 tagaaacaac gtttacggca tcagcagaag cacagattgc ttgctggctc gggattacga
     1981 aaaaaagact attgaatgaa aaaagtacgt acgcaacttt gaaagaaaag aaattactct
     2041 atcagtcgga ccggctttat caagcaatcg atccaatcat cgtgatgcat cttagtcgaa
     2101 cagcagcaga actgaatgta tacgaaacaa tgatgtttta cagctttttt gtttcttttt
     2161 cgatagtaga cgaagaaata ttttatcagt atgatttgac acggagcaaa aagttaccaa
     2221 ccgctgtttt agatacttac attcgggaaa cgatgttatg gcattatcgt cctcgtcgtt
     2281 tgaaaatcaa agaagaaaag gcagtaggct accaaatttc ccagatcaac aatgaatggt
     2341 ttttctttgt tggaaagatc gaagtctatg agcgtgatcg gcttttggaa cagcagcaga
     2401 aaatgctcgg gcattcctta acacagttac tggcgaaatt acaggaaaca gcggtgcagc
     2461 aattgccgag gaaaagagct gaagatagtg agcttagtta cctgatgatc cagtatgcca
     2521 atgttctttt gatgatcgat ttttatattg caaaaccagt agtgattgga atcgatttag
     2581 aaagtctgcc gatttatcgt attgcttttc aacaatatct gatcagagaa ctgaggggaa
     2641 tcggcggtat tgagattgga agttatgaag aaggtaaaga atatgatttg gtgatcacat
     2701 tttgccagag aaacaaaaaa cagtgtgaat attatctgtc agaatttgcg tcaccttacg
     2761 acattattcg attgaaaaga agaatagaga cgctgaaaaa agaaaagaat tgattaaaaa
     2821 atagcttcat aataaaaaac tgtcaaaaaa agaaaaaaag aataaaattt tgacagtttc
     2881 tttttttaaa acagtagtat gataagagta gttaaagaaa gcgtttgcat ataatggagg
     2941 gaatcacaat gacagaatca acgtatatca tggcgattga ccaaggtaca actagctcac
     3001 gagcaattat ctttgacaag aaaggaagac acatcggcag ttctcaaaaa gagttcacgc
     3061 aatatttccc gcaagaaagt tgggtggaac atgatgcaaa tgaaatctgg aattctgtac
     3121 agtcagttat tgccggagcg ttcatcgaat cagggatcaa accaaatcag attgctggga
     3181 ttggcataac gaaccagcgt gaaacaacag taatatggga aaaagataca ggacgtccaa
     3241 tctatcatgc agttgtttgg caatccagac agtcagctgg aattgcagat aacttaaaaa
     3301 atgaaggcta tcaagaattt ttccacgaaa aaacagggtt agtgatcgat gcctatttct
     3361 cagcaacgaa gatacgctgg attcttgatc atgtagaagg cgcgcaagaa cgagctgaaa
     3421 aaggcgaatt gatgtttgga acgatcgatt cttggttagt atggaaactg accgatggac
     3481 aagcacatgt tactgattac tcgaatgcca gtcggacgat gttgttcaat atccatcagc
     3541 tagattggga tcaggagatt ttagatttac taaatattcc tcgagtgatg ttaccaaaag
     3601 tcacttctaa ctcagaagtt tatggttata cgcaaggtta tcatttttat ggaagcgaag
     3661 caccaatttc agggatggca ggagaccagc aagccgcatt attcggacaa atggcattcg
     3721 aaccaggcat ggtcaagaat acttatggta caggttcatt tattgtcatg aataccggtg
     3781 aaaaaccgca attatccaaa aataatttat tgacgacaat tggttacggc ataaatggaa
     3841 aggtttacta tgctttagaa ggaagtattt ttgtggctgg ttcttcgatt caatggctga
     3901 gagatggact tcaaatgttg caaaaagcta gcgattcaga agacgcagcg aaacgctcta
     3961 caagcgaaga tgaggtttat gttgttcctg cattcgttgg gctaggtgct ccttattggg
     4021 atcaggcagc acgaggggct atgtttggct tgacacgtgg tacgacaaaa gaagatatca
     4081 ttaaagctac tttacagtcg attgcttatc aagtgagaga tatcatcgat acgatgcaag
     4141 atgacactgg aatcaagatt cctgtattga aagtagatgg cggtgcagca aacaatgaat
     4201 atctgatgca attccaaaca gatatcttga atgtaccgat ccaacgagca gaaaacttgg
     4261 aaacaacggc gctaggggca gcatttttag caggattagc tgtcggttat tggaaagata
     4321 cagacgagat ccgcgaattt tatgaagctg gcaagctatt tgaagttcaa atggaggaag
     4381 agcgccgaga aaaactttac aacggttgga aaaaagcagt aaaagctacc caagcatttg
     4441 aataaggagg aaatccatat gttttcaaac aagacaagac aagatagcat tcaaaaaatg
     4501 cagcaagaag aattggatct gttgatcatc ggtggcggaa tcactggtgc cggtgtggca
     4561 gtccagtcag cagcatcagg aatcaaaaca ggattgatcg aaatgcagga ttttgcagaa
     4621 gggacttcct ctcgctcgac caaacttgtt catggcggta ttcgttatct gaagacattt
     4681 gatgtggaag tagtagctga cacagttggt gaacgtgcag tcgtacaaag tattgcccca
     4741 cacatcccaa aaccagatcc aatgcttttg ccaatctatg agggcgaagg agcaacaacc
     4801 ttcaatatgt tctcggtcaa agtagcaatg gacctttatg acaaactggc aaatgtgaca
     4861 ggaaccaaat atgagaatta tacgctgaca ccagaagaag tattggaaag agaaccgttt
     4921 ttgaaaaaag aagggctaaa aggcgcgggt gtctatctag atttccgcaa caatgatgcc
     4981 cgtttagtga ttgataatat caaaaaggct gccgaagatg gggcttatct agtaagtaaa
     5041 atgaaagcgg ttggcttttt atatgatggc gatcaaatcg ttggcgtcaa agctcgtgac
     5101 ctgctgacag atgaagtgat cgaaatcaaa gcaaaattag tgatcaatac gagtggtcct
     5161 tgggtagata aagtacgaaa cttgaatttt acgcgtccag tctctcctaa aatgcgtcca
     5221 actaaaggga tccatttagt tgtagatgcg aaaaaactgc ctgtaccgca acccacatac
     5281 ttcgatacag gaaagcaaga tgggcggatg gtttttgcta ttccaagaga aaacaagact
     5341 tactttggta cgacagatac ggattaccaa ggagatttta cggatccgaa agtcacacaa
     5401 gaagatgtag attatctatt ggatgtgatc aatcatcgct atccagaagc aaatatcaca
     5461 ttgaatgata tcgaagcaag ctgggcagga cttcgtccac tgttgattgg taattctggt
     5521 tctgattaca atggtggaga taatggatcg atttcagaca agagcttcaa taaagttgtc
     5581 gatacagtaa gtgaatataa ggaaaacaaa gtttctcgtg ctgaagtaga agacgtgttg
     5641 aaccatttgg aaaacagccg tgatgaaaaa gcaccttcta cgatttccag aggtagttct
     5701 ttagaaagag aaccagatgg tttgctgact ttatctggtg ggaaaatcac cgattaccgt
     5761 aagatggcag aaggagcttt gcgattgata cgccaattat taaaagaaga atacggaata
     5821 gagacgaaag aaatcgactc taaaaaatat cagatttcag gtggaaactt cgatccaact
     5881 aaattagaag aaacagtgac agaattagca aaagaaggag tagcagccgg tttagaggaa
     5941 gaagatgcta cttatatcgc tgatttttac gggactaatg cccgacgtat ctttgaatta
     6001 gcaaaagaaa tggcacctta tcctggtttg agtctcgctg agtcagctcg gttacgttat
     6061 ggtttagaag aagaaatggt tttggcccca ggcgattatc tcattcgtcg tacaaatcat
     6121 ctgttgtttg aacgagatca gcttgatgag atcaagcaac ctgtgatcga tgcaattgct
     6181 ggatattttg aatggacaga agaggagaag gcgcaacaga ctaaacgttt agaagcattg
     6241 attgcagaat cagatctgcg ggaactaaag ggggagaaat aaatgaacag tgatatgact
     6301 caaatcatgg gggagtttat tgggacactg atcctagttt tgcttggaga tggtgtgtgt
     6361 gcggctgtga atttaaacaa aagtaaagca caggcttctg gctggattgt catcgcattt
     6421 ggttggggac tcgctgtaac aatggctgtc tatatttctg gattcatggg cccagctcat
     6481 ttgaatcctg cagtctctct agctatggcg atgacagggg caattagttg gaatcttgtc
     6541 gttcctttca tcattgcgca agttttagga gcttttgcag gggccattct tgtttggctt
     6601 tcttatttgc cacattggaa tgcaacgaaa gatgaaagcg cgattttagg aacgtttgca
     6661 acaggtcctg ctatacggaa ttatccagca aatgtgatca cagaattaat tggtacattt
     6721 gtattagttc ttgggttgct agcttttgga caaaacgaat ttgcacctgg aacgaacgta
     6781 ttcgctgtag gtggattgat cttagccatc ggtctttcat taggagggcc aacaggatat
     6841 gcaatcaacc ctgctcggga ctttggtcct cgattagcac atgcggtatt gccaatagct
     6901 aataaaggaa cttcagattg ggcatattca tgggtaccaa tcgcaggtcc aatgatcgga
     6961 gcaattatcg cggtaggtgt ctattcatta atggtgtaaa tctataaaac atactaaagt
     7021 ttgacaacca aaatgtgtct tgctatagtt aagtggaaaa taaataagac ctagaagaat
     7081 ggggtatgtt ttgtgagtgt agctggtatc taatcagtaa gtcgaatagc caataaacaa
     7141 agtttatttg gtgtgcttat gaaacggtac ctgtcaggaa aacaatatct ttgtattcta
     7201 ataaggatag gacatgactt ttttaagctg tgttctatcc ttttatttat ctctaggccc
     7261 ttatttattt tgggacaggt atctcttcaa gaaaaggaga gaatatggac aacgaaatga
     7321 ttagtcaatt acaatttgat cggatcaaaa aggaaatcca agcaagagca attggaaact
     7381 atagcaaaaa acgaatcagc gatatgacgg tctcgacaaa cctacaaact gtcttggatc
     7441 gtcaagaaga aacaagagaa gcacgcttga tcttagaaag cagtcagcat gttccattta
     7501 tggggttgcc gaggatcgat gcactgacag aacaagtcaa aaaagggttg attctgcagc
     7561 caaccgattt gatcgaatat gcggattttc ttagaagcag tcggatgatc acaaagtttt
     7621 ttgataagaa tcaataccag acacccttgt tgtttgctta tagcaaacat cttcctgatt
     7681 tgataaatgt agaagagttg attgaccaga aaataaaaaa taataaagtc agtgacgacg
     7741 cgtcacgtaa tttgcgtaaa gtacgcaaac aattacaact aatcgaaaaa gaaatccagt
     7801 caaaattact gaaattcttg cgtcatccga aaaacaaaga aatgatccaa gaagcaatga
     7861 ttgttcaaaa aggcgaatac tatacgattc ctatcaaagc cagctataaa aataaaatag
     7921 atggaacgat tatcgatgaa tcaaataaaa gaacgacggt ttttattgaa ccaactgtga
     7981 tttctaaatt gaatgaacac tatcagttgt taaaagctga agaaatcagt gaagaatatc
     8041 aagtcctggc ggcattgact ggtgctatcg cagaaaacga agaagtgatc gatttattga
     8101 tcgaaacgat cacggtgtta gatatcatct ttgctcgggc aaaatttagc cgagaaatca
     8161 atggaagcac acctagaatc aataaatcgg aacatatcat tatcaagcaa ggcagacatc
     8221 catttttacc tgagcatgcg gtaccattgg atgttgaaat cggtaaagac tatcgaggct
     8281 tgattatcac cggagcaaat gccggaggca aaacagtcgt gttgaagaca gtcggtttat
     8341 tgactttgat ggcgatgttc ggtatgcaag ttccggcaaa agaagggaca gagcttgctg
     8401 tctttgatga gattttcgtc gatgtcggtg accaccaaaa tttagaaaat gcgttgagta
     8461 cattttctgg tcatatgcaa aacatcgctg ctattttgaa aaagataaaa aggaatacgt
     8521 tggtactttt agatgagatt ggcagtggaa cagagccaaa tgaaggggct gcactagcga
     8581 tcgcaatcat ggaatctatg tatgagcaag gggcattgat cattgccacg acccattatg
     8641 gagaaatcaa gaaattcgcc ggagatcatg aagattttgt gccggctgct atggctttcg
     8701 atcgggaagc acttcgtcct aaatatcagt tgcgggttgg aagaaacagg agaaagccaa
     8761 gctttgtgga ttgctcataa aatggatatg tcgatgaaat tgatccatca agcagagcgt
     8821 tacttggcgg aaaaagccta tcggacacag aaaaaagaat ttcttcctaa gacagcagtt
     8881 acaaatagga aggagaataa aaaagaacgt cagctctttg caaaaggtga tcggatattt
     8941 gtcaatgaat accaaaaaga agcgttagtt tatgaagata taggtgaaga tacgattgat
     9001 gtatatttag acaaaaaaat aatccacgtg cctcgtcagc gtgttcgtct ggtgcgttca
     9061 gcagaagatc tgtacccgac aggttacgat ttagacagtc tgtttatcga ttataaaaca
     9121 cgtaaacggc agcgtgacct agagcgagga tcaaaaaaag ctcataaagt actagtaaaa
     9181 gaaatgcgca aacgacagga agaaagaaga gtaaacgatg aaaacagcaa gtgattatct
     9241 caaattagct cataatatca gtgtctgtaa agaaaaataa aaacagtgaa atcattggtt
     9301 ttattccccg tgtaagctaa aataaaaaga aaagagtgct gaggaggtag ggcgataaaa
     9361 tgaaattaag cacagactgg cgtaaagaaa tacaaacgat tccgaatctt ttatcgatat
     9421 tccgtatttt tttgctgccg atttatcttt attttgtgct gagacagtca ttttatatag
     9481 caggagcggt cattgttgtc tcaggtttgt ctgattatct agacggggtc attgctcgga
     9541 gatataatca ggtaacagac ttagggaagg tgcttgatcc gtttgcagat aaattgacac
     9601 aactttttct gattttgtct atggcatggt atcgaccgtg gctttggctg ttgtttggtc
     9661 tattcttgat aaaagaaggc tttatgtttg tagctggatt aatcgggcta tctaaaaata
     9721 tcaaattaag tggagcgaag tggtatggca aagtagcgac tgctgtcatc tatgttggca
     9781 tgatcctttt gctgcttttc ccagaattac caacgctgtg ggtgagagtc atttttgccg
     9841 tcatcactta cggtctttta caatcttttg tcttgtatgc tgtcgagtac cgaaagatgt
     9901 ttcaaagaaa ataaaaaagg ggtgtgacct atgtcgcatt cctttttttg ataaatagac
     9961 tcccagtagc tacttctcga taataagcca accgaaccaa aaacaggaga agttgttttt
    10021 ga
//
LOCUS       DACENJ010000090         9907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.091, whole genome shotgun sequence.
ACCESSION   DACENJ010000090 DACENJ010000000
VERSION     DACENJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.091"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(119..343)
                     /locus_tag="IW748_001603"
     CDS             complement(119..343)
                     /locus_tag="IW748_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ1104516.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(541..2121)
                     /locus_tag="IW748_001604"
     CDS             complement(541..2121)
                     /locus_tag="IW748_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1104517.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2522..3898)
                     /locus_tag="IW748_001605"
     CDS             complement(2522..3898)
                     /locus_tag="IW748_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1104518.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4026..5144)
                     /locus_tag="IW748_001606"
     CDS             complement(4026..5144)
                     /locus_tag="IW748_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1104519.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5286..5720
                     /locus_tag="IW748_001607"
     CDS             5286..5720
                     /locus_tag="IW748_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1104520.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5798..6331)
                     /locus_tag="IW748_001608"
     CDS             complement(5798..6331)
                     /locus_tag="IW748_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1104521.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6425..7603)
                     /gene="mutY"
                     /locus_tag="IW748_001609"
     CDS             complement(6425..7603)
                     /gene="mutY"
                     /locus_tag="IW748_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1104522.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7596..8393)
                     /gene="recX"
                     /locus_tag="IW748_001610"
     CDS             complement(7596..8393)
                     /gene="recX"
                     /locus_tag="IW748_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1104523.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8500..9882
                     /gene="rlmD"
                     /locus_tag="IW748_001611"
     CDS             8500..9882
                     /gene="rlmD"
                     /locus_tag="IW748_001611"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1104524.1"
                     /translation="MAETIVRTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
BASE COUNT         2896 a         2038 c         1700 g         3273 t
ORIGIN      
        1 aagcagaagc aactccttcg gaaataagct gaaattcacg aaaattgctt ttcaaatttt
       61 tgtgaattcc agcttatttc ttggagttaa acgcttctgt ctcagcctct ttttctttct
      121 agctttttga tttttctctt ttcttttgta cttttctctt ttcattttca gttctacgcg
      181 tgtctttatt tttaactttt tcctctttat ggatgattga tcgtactttt tgtgcaattt
      241 gctcgttttt gtcccagtct tttacatcta ataatcgaca taatcgtaac atctcttctt
      301 ttttctgttt ttttctttca cgtctttgcc attctgtttc catgtgtcac actctccttt
      361 ctttaatcat atgtacgcaa aataattttt atattttttt gattagacat ttctatgatt
      421 ttaatatagt aaaaagagcc gtcacggaaa atccgtcacg gctctaaaca aaatatgaga
      481 ggctgttttt gtttggctat tcttattact tgatgcaggg cagcttgtgt caagacttct
      541 ttacatcaaa ctttttaatt ttacattggg atatttatcc gcaaaccagc gttctgcaaa
      601 ttggttctcg aataagaaca acggttggtc gaaacggtca cgagccaaga tattgcgact
      661 tgaactcatc ttctcatcca aatcttctgg atcgatccag cgagcgattt tgtgtcccat
      721 tggagacatg attacttcgg cgttatactc atttaacata cgatattgga atacttcgaa
      781 ttgtagctga ccaacagctc caatgatata ttcttccgtt agatatgttt tataaagctg
      841 aatcgctcct tcttgaacta attgatagat tcctttgtgg aaggattttt gtttcatgac
      901 attttttgca gtcactttca taaacagctc tggagtaaat gaagggagtt cttcataagc
      961 aacttttagt tttccttcga acaatgtatc cccaatctga taattgcctg tatcatatac
     1021 accgatgata tcgcctgcta ctgctttttc gacgttttct ctcgaatctg ccataaattg
     1081 tgtgacattg cttaatttca gttttttatt tgtacgttcc aaccagacat ccattcctcg
     1141 ttcgaatgtt ccagaacaga ttcggacaaa agcgattcgg tcacgatggg ctggattcat
     1201 atttgcttgg atcttgaata cgaaacctga aaattcttcc tcataaggac ttaccatctg
     1261 atcatcttgt gttttgtgtc cataaggagc tggtgccaat tcaacgaaag tctctaaaaa
     1321 agtctgtaca ccgaagttag tcaatgcaga accaaaaaat actggtgtct gatcgccacg
     1381 agcaattttt tcttgattaa atgcatctcc agcctctttg attagttcta cttcttctaa
     1441 gacttgttca tataaggaat tttcagttag aggatgattt tcgggaagca gtccatcttc
     1501 atccaattct actaatcgtt catcctgata attttccgga cgataaaact ctacacgatt
     1561 atggtagata tcataaagac cttctagccc tttacccata ccgattggcc aattcatcgg
     1621 ataagattcg atatctaata gctcttctaa ttcttctaac aattccaatg gttcacgacc
     1681 atcacgatct agtttgttga taaaagtaaa tatcgggatg ccacgttttt ttacaacttg
     1741 gaataatttc tttgtctgtg cttcgatccc ttttgcacta tcgattacca tgaccgcact
     1801 atccacggcc atcaaagtac gataagtatc ttcagaaaaa tcttcatgtc ctggtgtatc
     1861 caagatattt actcgtttcc cttgataatc aaactgcata acagaactag taacagaaat
     1921 cccacgttgt ttctcgattt ccatccagtc tgatttggca aaattacctg tttttttgcc
     1981 ttttacggtc ccggcttgac ggatagcccc accaaacaat agtaattgtt ctgtaatcgt
     2041 tgttttccca gcatccggat gggaaatgat cgcaaaagtt ctgcgattgt ccacttgttg
     2101 ctttaatttt ggattattca taacctaact ccatttcttc cattcaatta tttatatttt
     2161 ctctaggcac agattgtccg atctcttaaa gaaagacaat ttcatatagg gctgtgacaa
     2221 aagccgtgta gcagtctctt tttccgagta aacggcgttc tagagacagc ctatcagcaa
     2281 taagcccacc caagccaaaa atacgaaaag ctgtttttgc ttggctgctc ttattactta
     2341 atgcaggata gctttttcac aacctttgtc aactactaca actgaatcag tatagcatgg
     2401 attcagcgaa tgaaaaagcc accaaaccat aagagtcaaa tatttttttg tcagctgact
     2461 aaaagaaaag acgtgttgct tgtttattca agcaacacgt ctttcataac caagtttcca
     2521 attatcttct tggctcgtca tcttcgaatt cttttctgaa gtatcttcgt tcttcttcag
     2581 gttccgcttc tgtttcttcc ggatcatgaa aaatgacacg gattttaagg attcgagaac
     2641 cttccatttc ttcagaaacc agcgtaatat tatcgacatc aaacgataac ttttcgcctt
     2701 catccggaat catgcctaat gctgtaatca aataaccagc catcgtatct acatcactca
     2761 tatgcagatc actgtcaaaa gcttcattga actcatcaat cagcatacgt ccttggatga
     2821 tatattcgtg ttcatcgatt ttttcataaa gattctcaac ttcatctgat tcatcatcga
     2881 tttctccgac gatttcttct aaaaggtctt ctagtgttgc taacccaacg acgccgccat
     2941 attcatctaa taaaatagcc atctgattct gcgtcttttt catttcatat aacagatcat
     3001 caataaagac cgtttcagga acgaataaag gctcctgcat gattttcttg atatctaaat
     3061 tatcaaagcc gaatttatgg gcagctttta gtaaattctt tgtatgaagg attccgacta
     3121 ctttatcttt gtcttcgtta taaacaggga ttcttgaata gttttcggat aaaacttcat
     3181 tcacattttc ttccacagtg tcgttgatat caatcataaa tgcatccgtt cgtgggacca
     3241 tgacttctcg ggcaactttc gtatcaagag aaaaaacccc ttggagcatt tctaattctt
     3301 cattattcaa tacgccttcg ttttcaagca tataacgcat ttcatctctg gtcatttttg
     3361 aatcttcatc atcaaaagtc atcggcgtga tacgcgccaa taaacttgtg gaagcagaaa
     3421 gcagccagac gaagggctta gctactacac caatcaagcg tatcagccct gaagtgaact
     3481 ttgcgacttc ttctgattta tttaaagcaa tacgtttagg gtacaattct ccaaaaacaa
     3541 tcgatacata ggtcaaaata gccagcacaa tgatatttgc aatatttttt gccgctgtgc
     3601 cgccacctaa taccggagct aaccgttccg ataaagtatt agctagtgaa gcaccagaca
     3661 aaatgttgac caaagtgatt cctacttgga ttgtcgatag gaaattattt ggattctgca
     3721 atatttttaa aagcttttga gatttcacat ctccttcatc agctttttgc tcgattcgat
     3781 tcttattgat cgaaacaaca gcaatttcgg aagctgctaa aaaagcattg atcaatgtca
     3841 aaacgatcaa taataaaatc tgcgctaaca gcgactgact ctcagggtca gcattcataa
     3901 tcgcattctc tccttataaa attaattttt agtttacgat agaaaaaaac gtggcactat
     3961 agccacgtta aaatggtatc acaaagcaat tttctttgca ataaaaacaa gtgtactaaa
     4021 actacctacg aggtcgtagc aattttcttc ttgttttcat tttgcttgtc ttctttgacg
     4081 attttgatta tataggaaat gatcgtcttg cagattgcgt aaaaaggaac acctaagaag
     4141 ataccaagca atccggcaat atttccagct actagaagaa cgataatgat cgttaatgga
     4201 tggattttca gtgatttccc aattacatta ggataaatga tattcccatc caactgttgg
     4261 acgattagca caaccacaca acaaagtaat gcttttaccg gatcattgaa gactgttact
     4321 agaaatgcag gtgctagtcc tagataaggt cctaaatatg gaattaggtt tgtcaatccc
     4381 gcaatgaccc caaataagaa agcataatcc acaccaatta ccatatatcc taaaaatgtg
     4441 aaggtaccaa cgaataaaca ttcgattgct tgtccgctaa tgtaattagc caatgtttta
     4501 ttcagttgcc ccaataactc cacaatttgg tttcttcttt tttctgggaa aaactgttgg
     4561 atgtttggca ctagtttttc tccgtctttc aacatataga acaagatgaa tggtgctgta
     4621 atgattacta cggttgccga agcgatcgtt ccaacgatcg aacctaaact attggatact
     4681 ccactaagga attgctgaat gattgtcccg taagaaatgt ctaattgctc aaaatattta
     4741 ttgatatcta cttccttgaa caaaggcaat tcggctacct cttttaacca gctttctacc
     4801 tgtttgatga aatcaggcat attagatgcc aatgaggaaa tttgctgtac tagacttggg
     4861 atgacactaa gtaaaatcca aataattgct gcaactaaaa ggagcagcac aattgcgaca
     4921 gcccatattc ttttgatttt tgctttcatc aacagcttga caataggatt caataaatag
     4981 taaagaaagc ctgcaatcag tacaggtgca aataacgtgg aaaaaaatgt cccgatcggt
     5041 tcgaaaataa agtttatttc tgatgaaatc atgatcaatg cagctatgat caataattca
     5101 agcgaccaaa acattagttt agactgtcta agtttttcaa acaatagatt tcctcctttc
     5161 tttcttacta gtatatatta tattcgttaa aaattctttt attctttttt tatttttgaa
     5221 aacttgccat actttttatt tgttttattt tccagtatac tatacgaaag gaaggtggct
     5281 agaccatgaa agttgtacaa acaaaagata caatgagcaa tatttactta gatgctgtac
     5341 ggatcaggca tcaagttttt gtagtcgaac agggtgtgcc tttgtctcga gaaatagaca
     5401 aagatgaagc ccattgtatc cacttcgtcc tgtactcaga taaaaaagaa ccgcaaggaa
     5461 ctgtacgtct tttgccttta gaaaacggaa aaatgaaatt acagcggatg gcgattcttt
     5521 ctgaatacag acaccaaggg ttaggaaaaa tattgattga agaagcagaa aactttgcta
     5581 agaatcaagg ctataatacg attttattag gggcacagtc cacagctgaa actttttacg
     5641 aaaaactagg atatacagct tacggcgatc cttttgaaga tgctggcatg cctcatattt
     5701 atatgaaaaa gactttataa aaatttattt cgaaaaaagc aaaaaacagc aagcttttga
     5761 gaaaaatctc tcactggctt actgttcttt tttgtgttta cttacgcgac aattgcttgt
     5821 aacggttata ccaaatatca atatagcctt ccgagaacgg gccatctcca ttattgatcc
     5881 agtcaaccag aatcttcaca ttttctttta ggatgaagtc aatatcgttc gggtaattca
     5941 tcattttgct gtgcatctca tattcatcga catccaatag tcgtttctcg ccgtcaggaa
     6001 ataccttgat atccaagtca tagtcgatat atttcaaggc ctcatcgtcc agcaaaaaag
     6061 gtgaagctag attacaataa taggaaaccc ccttttctct gatcattgct attacattga
     6121 accagtattt tttatgaaaa taaacaatcg ctggttcgcg cgttacccat cggcgtccat
     6181 cagattcagt gactaatgtg tgatcattca cgccgatcat agagtgttcg cttgttttta
     6241 ataccatggt atctcgccac gttcgatgca atttgccgtc gtgcttgtaa ctttggatcg
     6301 tcacaaactc tccttctttt ggaactccca tagcaaccct tccttaagca gcattttgtg
     6361 ctttgtgttt gggtgttcat tacaccgaca tcataattat tatatcaata aaaatgagct
     6421 ttgcctagaa aggattccta ttgtttcgat ttttctctaa ctgatctacc agtttcattt
     6481 ggaccttagg aaaaacgacc gaatcaaatg catcaggctt caaccacttc gaagttttgt
     6541 tctcggtaaa ttcttgttct gctccctcgg ttgcccgtcc gtaaaaaagg agaacatgcc
     6601 atttcagatg actaaaaata tgagtaactt ctcctaaatg tcgtgtctgc caaacaaaga
     6661 gttcctgctt ttcaaatggg agattttgct cttcttctgc tactaggtca tcaaacagat
     6721 cgatttctgg ctttgtttcc cattcttttt tcaatcgctc atactcctct tgtgtcactt
     6781 ccatcattgg aaatgtccac atattcgcca gcaatttttg gctatctctt tcttcaaaat
     6841 aatacgctcc agaatgattt tgaagagctg cactgatata ataaacgtct tttggctttg
     6901 ctttttttgt ttttacagga aaactcgttt ggattcctcg tttgttagct aaacaaaagg
     6961 cttggatcgg acaagtctca cactttggag aagttggtgt acagatagct gaacctagat
     7021 ccatcattgc ttggttgaat tcacctggat gcttttcatc aatgattttc cgcatcgctt
     7081 catcaaagat tttcctgcta gaagcttttg cgatgtctgc ttcaatgcaa aacaatctgc
     7141 tgactactcg catcacgttg ccgtcaactg caggttctgg aagtccaaat gcgatacttg
     7201 caatcgcgcc tgtcgtatat ggtcctattc ccttcaatga gctaatttct tcaggcgttt
     7261 gcggcatttc cccatcaaat tctgacatga tctgtttagc agcagcttgg atgttccggg
     7321 ctcttgagta ataaccaagg ccctcccatg cttttaaaag cttttcttct ggagcatttg
     7381 ctagctcttc aattgtagga aaccattcca tgaaacgata aaaataatcg atcactgtgt
     7441 ctactctggt ttgctgaagc atgatctcag agatccagat acgataagga tcacgattat
     7501 agcgccaagg caggtttctt ttttcttgtt cataccattg aatgaactga tcttgaaatt
     7561 cttttgtttc tttatctgtc catttttccc attctttatt cactttcctc ttccttttcc
     7621 gtaatgaatc gctggatcat atccaaatca aatccttttt gatataagct ttgctttact
     7681 tttagatttc tttttttagg ttcaaacctt aaatttcttc gccatagttt atctccttgt
     7741 ttgactaaat aatcatattc tatttcttga tctgcttctt gcggaagatc agctatcgct
     7801 tgttggatca cttcttgaga aaagcctttc gtcatcaatg tttgttggat ttttctcaat
     7861 aattcttttt ggctttttcc gtgatttttt ccaaacgctt tttcggctgt ctgatgtgca
     7921 ttttgaactt ggtcttcaaa tgtatattgg ctgagcgctt gttcaatgat ttcaggtttc
     7981 aaaccttttt gctgcatctg ctgcgctaat ttctttggtc ctttgtcact taagcgcata
     8041 ttcgtgcgca cataactttc cccatagacc aaatcatcga caaggtccaa ttcttttaag
     8101 cgggctacga ttttatttcg atcttcttga aggatttcct tttcttttaa gtatgttcgg
     8161 atttcttttt ccgatcgcaa ctgataactt aagtaattca ttgctaggtg aaaaccgaaa
     8221 tcagctccgc tactcttctt gatctcttgg atagtttctt cgtctaattc ccgtcctttt
     8281 aataatcgat agcgtactaa tacatcttca gatacttgta aagcttctcc ttcagataaa
     8341 tctacgcgat agaaagggcc tttgccttta ctgattctcg taacggttaa cataaacatc
     8401 ctcctcgaac atatattctc tatcatacca cagaaaatcc ccgtaatcag gtattatatg
     8461 ttagaataaa ccacagaaaa aagaaaaagg gagatactta tggcagaaac aattgtcaga
     8521 actggacaaa cggttccttt gaaaatcaag cgtttaggca taaatggtga aggaatcggt
     8581 tatttcaaac ggttgatcat atttgttcca tatgcactac caaaagaaga agtactagtg
     8641 aaaatcacga aagcaactcc tcgctatgca gaaggagaat taataaaagt caaaaaaaca
     8701 agcaaagatc gcgtagtggc accatgtcct gtttactatg aatgcggtgg ctgtcaatta
     8761 caacacttag cttatcaagc tcaattggat tttaaaaagg atctgctttt gcaagcattg
     8821 gaaaaattca aaccagcagg ctttcgatct tatcaacttt tgccgacgat cgggatggat
     8881 gagccttggc attatagaaa caaagcacaa tttcagttac gaaaaaataa gcagactcaa
     8941 aaaattgaag ctggtttata cgaagcaaac tctcatcagt tagtccctat aacagactgc
     9001 ttggttcaag aacctcgaac acaaaaagta atgaatactg ttgttcaact attgaataaa
     9061 tttgatgcac ccatctatga tgaacggaca aacagtggaa tcttccgcac aatcatggtt
     9121 agaatcggtg ttaaaactgg ggagttacaa gttgttttta ttactcgcag taaaaaattc
     9181 ccgcaaaaaa acgcaatgat ccgtgagatc aatgaacgac tgcccgaagt tgtttcaatc
     9241 atgcaaaatg tccaggcaca aaaaacgtct ctcgtgatgg gggatgaaac gatccactta
     9301 tggggaaaag aagccattga agaacaaatc aatgaagtga ctttcgatct ttctcctcgt
     9361 gccttctttc aactcaatcc tcagcagacc gaattgcttt atggcgaagg aatcaaagca
     9421 ttagatgtcc agcctaatga aacgatcgtg gatgcttatt gcggagttgg gacgatcggc
     9481 ttaagtgtag caaaacaagc caaagaagta cggggaatgg ataccgttcc tcaagcaatc
     9541 gctgatgcaa aaaaaaatgc ggaaagatta ggatatcaca acactcgata tgaagtcggg
     9601 acagcagagc aagttctgcc taaatggttg aatgaaggtt ttcagcctga tggtatcatc
     9661 gtagatccac cacgtacggg tttggataac gcattgatcc aatcattatt gaaataccct
     9721 ccaaaaaaac ttgtttatat ctcctgtaat gtttctacac ttgccagaga tctggttaat
     9781 ttaagtaaaa agttcaagat tgaatattta cagtccgtcg acatgtttcc tcagactgca
     9841 cgttgtgaag ttgtcgtgaa gatgacgaga ctcgctgact aaatgaaaga ggttgtgaaa
     9901 aagtctg
//
LOCUS       DACENJ010000091         9809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.092, whole genome shotgun sequence.
ACCESSION   DACENJ010000091 DACENJ010000000
VERSION     DACENJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.092"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(31..2151)
                     /locus_tag="IW748_001612"
     CDS             complement(31..2151)
                     /locus_tag="IW748_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1104525.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2381..3559
                     /locus_tag="IW748_001613"
     CDS             2381..3559
                     /locus_tag="IW748_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1104526.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3575..4333
                     /gene="aroD"
                     /locus_tag="IW748_001614"
     CDS             3575..4333
                     /gene="aroD"
                     /locus_tag="IW748_001614"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1104527.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4356..4841
                     /gene="ybaK"
                     /locus_tag="IW748_001615"
     CDS             4356..4841
                     /gene="ybaK"
                     /locus_tag="IW748_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1104528.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4912..6165)
                     /locus_tag="IW748_001616"
     CDS             complement(4912..6165)
                     /locus_tag="IW748_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1104529.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6282..7631
                     /gene="gdhA"
                     /locus_tag="IW748_001617"
     CDS             6282..7631
                     /gene="gdhA"
                     /locus_tag="IW748_001617"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1104530.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7743..9089
                     /locus_tag="IW748_001618"
     CDS             7743..9089
                     /locus_tag="IW748_001618"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1104531.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9397..9783)
                     /locus_tag="IW748_001619"
     CDS             complement(9397..9783)
                     /locus_tag="IW748_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1104532.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3126 a         1782 c         2010 g         2891 t
ORIGIN      
        1 tttgtcgctg ctccttttaa atttatttta tcattcggaa gaacttgtgg tcgttccgtg
       61 aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt tgtgttcact
      121 aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt tctctaagcc
      181 attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg tatagaaccg
      241 aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa ctttttcttt
      301 cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt caccagtttc
      361 gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat caccatcacg
      421 gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact gaatataatt
      481 ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta
      541 agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag ggacacgctg
      601 catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta attcagctag
      661 atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga tgactgaatt
      721 atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg tatctaaata
      781 attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta acgggaatcc
      841 tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa tgaattttga
      901 ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa atggtttatc
      961 atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag aatcaaagaa
     1021 ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac cagcgttccc
     1081 atggaatacc gcactaatgt aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa
     1141 agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt
     1201 ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga aaaagttgtc
     1261 aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa cttcatgctg
     1321 gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc tttctaaatg
     1381 gatataaatc acattttttc ctctagcgag cccgaacaaa tcgtcatttg gtgctgcata
     1441 atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt ctgcccgcac
     1501 ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca agccaagata
     1561 tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat cagcttccgc
     1621 cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa aagccattct
     1681 tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa cagtcaagat
     1741 aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg cactttctcc
     1801 cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa
     1861 ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat
     1921 catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa gtaataacaa
     1981 tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt cgattccgag
     2041 atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga
     2101 aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca agataatctt
     2161 cctttctgca cttcttaatc acagaagtgt cctgaaacca tgttacacag ctcacttatt
     2221 ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt ttatgctgtt
     2281 tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt cctatgtttt
     2341 ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa
     2401 aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag accttgctca
     2461 tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc aatttatcgc
     2521 aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc agcagaacat
     2581 cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt
     2641 ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag gagacgggat
     2701 cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt ataacgaaac
     2761 gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc cggaaatcat
     2821 aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc agttcattta
     2881 cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta
     2941 tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac ggggatcatt
     3001 agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata ccggcgcctt
     3061 ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg
     3121 cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg atcagcaaaa
     3181 aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac aatttttata
     3241 tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag tctttcgcgt
     3301 agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag aagacgggat
     3361 acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa caatgattga
     3421 agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc gtctgccaaa
     3481 agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt ttgtttatca
     3541 aattaaaaat aaccattaga aggagcgatg cagaatggtt cgggtaaagg gattgaatat
     3601 aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt
     3661 acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc gagtcgatca
     3721 ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta tccatgagat
     3781 gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg gagaacaaga
     3841 catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag gattagtaga
     3901 tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga tagaggaaat
     3961 caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa caccagcaga
     4021 ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata tcggaaaact
     4081 ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga ctagacgggc
     4141 aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg gtaaaatttc
     4201 tcgtctggtt ggcggaacga cgggttccgt tatgacattt ggttccttaa gcagcgattc
     4261 tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc aattattaaa
     4321 tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa
     4381 cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac gaatatgagt
     4441 gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc
     4501 gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt gctgtgatac
     4561 cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag
     4621 aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt ggctgctcgc
     4681 ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa agctatgaac
     4741 aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct caagatatct
     4801 gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt ataaaagaaa
     4861 gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac attatgctct
     4921 ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc
     4981 gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat caatgatttt
     5041 actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag ttacaaatga
     5101 tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata gttgaacaaa
     5161 atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt caagccgctc
     5221 aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga ccaagttctt
     5281 tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg
     5341 gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt tattgacaaa
     5401 acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat
     5461 cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa
     5521 agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca agggggactc
     5581 tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt acccatctcc
     5641 agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg caggagcatg
     5701 gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga tcaatttctc
     5761 tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga gcaatgcttc
     5821 gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct cttcttttaa
     5881 atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt gcgcaaacgt
     5941 actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa caggacgatt
     6001 gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg cttttttgtt
     6061 tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc
     6121 gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct cacctcaagg
     6181 aaagtgtagc atatctgaag aaagaaaaca gaaaaaaatg ttttgagcca aaagagaaaa
     6241 tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac gcaacagaat
     6301 atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa gaatttttac
     6361 aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat cccgaatata
     6421 tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa tttcgagtgc
     6481 cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc caatacaact
     6541 cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat ctaagtatct
     6601 tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg ccaatcggtg
     6661 gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag atcatgcgtt
     6721 tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt gatgttcctg
     6781 cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa tataaacgat
     6841 tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg ggaagtaaga
     6901 tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta ttgaacgaag
     6961 aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat gtagccattt
     7021 atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca gattctagcg
     7081 ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc aaagaaatca
     7141 atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat catgctggag
     7201 aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct actcaaaatg
     7261 aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc gtagcagaag
     7321 gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca ggcgtgtggt
     7381 attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta gaaatgagtc
     7441 agaacgtcca gcgattgact tgggaaagag agcaagtgga tgagcagctg gatcaaatca
     7501 tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac gaaaaaaatc
     7561 tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg gcagcagaag
     7621 gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt tatttgcttg
     7681 aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa ggaggagaac
     7741 aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac gaacaagagt
     7801 tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa ggtactggcg
     7861 caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa gaagaatttg
     7921 cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta gttgttatcg
     7981 gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat cacacattct
     8041 tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc ggaaattcaa
     8101 tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac ttttcagtaa
     8161 acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt gtattcaaag
     8221 agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac gctacaacag
     8281 ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa actttcgtga
     8341 ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg ttaccaatcg
     8401 ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca agcaaagcat
     8461 actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg cgcaacattc
     8521 tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga atgcaatact
     8581 tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa aaaggcatct
     8641 atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc atccaagaag
     8701 gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa tcaatcacaa
     8761 ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa gaagtggact
     8821 tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt gacgtgccta
     8881 acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca atgtacttct
     8941 ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc gaccaaccag
     9001 gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccaggc tttgaagatc
     9061 ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat ttaaataatc
     9121 agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt actcaaacat
     9181 actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag tcccttgttt
     9241 gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta agaacagtga
     9301 ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag caaaaaagca
     9361 ctcacttcga gtgctttttt gcgatatatt cttagcttaa gataggccta atttttcttt
     9421 tacagatgag ggtaccttat cgctggtaat tacttttttc tctatgatcc gcttttcgct
     9481 tgctttcaac tgcaagatcg ttccttcggg gtaagcagaa ccattggcag agtcaccaga
     9541 agtaatgaca tcgaaatcta actgtctttt ttcaccgttt tcagcataag catctacgtt
     9601 gtatttaaaa cctcttccaa ttacttcccc attatctgct ttgatatcag tttctgcagg
     9661 cattggagct ttgactaccg cataataatc tttacctaca taagtagact gatagtagtc
     9721 ccaacctttc catcctataa aagcaatcaa tatgataacg atcaacccta aaattttttt
     9781 catgaaagat ctcctttttt atttttatt
//
LOCUS       DACENJ010000092         9739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.093, whole genome shotgun sequence.
ACCESSION   DACENJ010000092 DACENJ010000000
VERSION     DACENJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.093"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            175..660
                     /locus_tag="IW748_001620"
     CDS             175..660
                     /locus_tag="IW748_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1104533.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            653..1336
                     /locus_tag="IW748_001621"
     CDS             653..1336
                     /locus_tag="IW748_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1104534.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1462..4107
                     /gene="polA"
                     /locus_tag="IW748_001622"
     CDS             1462..4107
                     /gene="polA"
                     /locus_tag="IW748_001622"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1104535.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4152..4988
                     /gene="mutM"
                     /locus_tag="IW748_001623"
     CDS             4152..4988
                     /gene="mutM"
                     /locus_tag="IW748_001623"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1104536.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            4952..5581
                     /locus_tag="IW748_001624"
     CDS             4952..5581
                     /locus_tag="IW748_001624"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1104537.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5633..5965
                     /locus_tag="IW748_001625"
     CDS             5633..5965
                     /locus_tag="IW748_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1104538.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6277..6768
                     /gene="nrdR"
                     /locus_tag="IW748_001626"
     CDS             6277..6768
                     /gene="nrdR"
                     /locus_tag="IW748_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1104539.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6791..8164
                     /locus_tag="IW748_001627"
     CDS             6791..8164
                     /locus_tag="IW748_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1104540.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8164..9090
                     /gene="dnaI"
                     /locus_tag="IW748_001628"
     CDS             8164..9090
                     /gene="dnaI"
                     /locus_tag="IW748_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1104541.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9219..9659
                     /locus_tag="IW748_001629"
     CDS             9219..9659
                     /locus_tag="IW748_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1104542.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         3361 a         1700 c         2146 g         2532 t
ORIGIN      
        1 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
       61 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
      121 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
      181 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
      241 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
      301 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
      361 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
      421 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
      481 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
      541 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
      601 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
      661 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
      721 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
      781 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
      841 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
      901 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
      961 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
     1021 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
     1081 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
     1141 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
     1201 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
     1261 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
     1321 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
     1381 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
     1441 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
     1501 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
     1561 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
     1621 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
     1681 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
     1741 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
     1801 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
     1861 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
     1921 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
     1981 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
     2041 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
     2101 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
     2161 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
     2221 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
     2281 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
     2341 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
     2401 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
     2461 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
     2521 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
     2581 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
     2641 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
     2701 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
     2761 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
     2821 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagataaa
     2881 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
     2941 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
     3001 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
     3061 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
     3121 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
     3181 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
     3241 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
     3301 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
     3361 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
     3421 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
     3481 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
     3541 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
     3601 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
     3661 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
     3721 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
     3781 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
     3841 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
     3901 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
     3961 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
     4021 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
     4081 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
     4141 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
     4201 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
     4261 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
     4321 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
     4381 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
     4441 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
     4501 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
     4561 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
     4621 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
     4681 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
     4741 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
     4801 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
     4861 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
     4921 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
     4981 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
     5041 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
     5101 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
     5161 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
     5221 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
     5281 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
     5341 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
     5401 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
     5461 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
     5521 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
     5581 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
     5641 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
     5701 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
     5761 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
     5821 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
     5881 cccagaaagt gaccacattt ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
     5941 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
     6001 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
     6061 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
     6121 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
     6181 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
     6241 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
     6301 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
     6361 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
     6421 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
     6481 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
     6541 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
     6601 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
     6661 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
     6721 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
     6781 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
     6841 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
     6901 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
     6961 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
     7021 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
     7081 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
     7141 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
     7201 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
     7261 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
     7321 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
     7381 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
     7441 aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
     7501 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
     7561 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
     7621 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
     7681 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
     7741 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
     7801 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
     7861 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
     7921 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
     7981 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
     8041 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
     8101 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
     8161 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
     8221 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
     8281 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
     8341 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
     8401 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
     8461 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
     8521 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
     8581 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
     8641 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
     8701 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
     8761 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
     8821 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
     8881 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
     8941 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
     9001 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
     9061 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
     9121 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
     9181 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
     9241 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
     9301 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
     9361 gacaaagcat atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa
     9421 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
     9481 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
     9541 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
     9601 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
     9661 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
     9721 taaggagata tgggaattt
//
LOCUS       DACENJ010000093         9704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.094, whole genome shotgun sequence.
ACCESSION   DACENJ010000093 DACENJ010000000
VERSION     DACENJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.094"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(50..2284)
                     /locus_tag="IW748_001630"
     CDS             complement(50..2284)
                     /locus_tag="IW748_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1104543.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2763..3029
                     /locus_tag="IW748_001631"
     CDS             2763..3029
                     /locus_tag="IW748_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1104544.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3029..4756
                     /gene="ptsP"
                     /locus_tag="IW748_001632"
     CDS             3029..4756
                     /gene="ptsP"
                     /locus_tag="IW748_001632"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1104545.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            4887..5966
                     /locus_tag="IW748_001633"
     CDS             4887..5966
                     /locus_tag="IW748_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1104546.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            5983..7206
                     /locus_tag="IW748_001634"
     CDS             5983..7206
                     /locus_tag="IW748_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1104547.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7295..8182
                     /locus_tag="IW748_001635"
     CDS             7295..8182
                     /locus_tag="IW748_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1104548.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8268..8498
                     /locus_tag="IW748_001636"
     CDS             8268..8498
                     /locus_tag="IW748_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1104549.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(8555..9520)
                     /locus_tag="IW748_001637"
     CDS             complement(8555..9520)
                     /locus_tag="IW748_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1104550.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3129 a         1712 c         1966 g         2897 t
ORIGIN      
        1 tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt tattcagccg
       61 tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg atttttccat
      121 ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa aattcagcga
      181 taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc atagatggat
      241 catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg tgaagttgct
      301 gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc atcaagtttt
      361 cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat tctggtgtaa
      421 agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc gcaccaaaac
      481 caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg atcgtatctt
      541 taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag atttgcaaga
      601 acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca agactgtaag
      661 gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca taacctggag
      721 gagaaccaat caatttagag acactatgtt tttccatata ttcagacata tcgaaacgaa
      781 tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc tctgttttac
      841 ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt ttgtttaatc
      901 caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct tgtccgatga
      961 catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt tcttttaatt
     1021 cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt tcggtaatca
     1081 caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta ttgatctgat
     1141 cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact ttttgttctt
     1201 ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc gtcaagtttt
     1261 tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag cgatcttgga
     1321 cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat tttacatgat
     1381 gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct tcgacagttg
     1441 gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt tcgatgatgc
     1501 ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct aaagctggtt
     1561 ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg actatttcgt
     1621 gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca atcaattttt
     1681 gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat acgacatcta
     1741 aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg attttttgtg
     1801 ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag acaggattgt
     1861 tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg cgtccgatca
     1921 caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat tctcccaata
     1981 aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt ccgccaaatt
     2041 gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac atggaacgga
     2101 aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta gtcagacctc
     2161 cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt tgtgtccgct
     2221 gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag ttttgacaaa
     2281 gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac atctcgtttt
     2341 tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag aaaaggtgaa
     2401 atctctattg accattcctg acctttgact cttattatac taaaaataaa aagggaagca
     2461 agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa acgtcaggag
     2521 aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg aagaaaagga
     2581 tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa aactgttgta
     2641 tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt aggattctct
     2701 gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg agaccgatta
     2761 atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct cgtccagcta
     2821 cactattagt acaaacagca agcaaattta actctgatgt aaacttagag tataaaggta
     2881 aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt caaggttctg
     2941 atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca atcgttgaaa
     3001 caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga ttgccgctag
     3061 tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa cattcagtaa
     3121 ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat tagctaaatc
     3181 tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg aagcagaagc
     3241 gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag gtcaaatcaa
     3301 gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag taacggatat
     3361 gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc gtgctgcaga
     3421 tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt taccaaatcc
     3481 atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa gtgatacagc
     3541 acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac gtacttcaca
     3601 ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa aagaaattac
     3661 tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg atgtaatcat
     3721 cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt acgcagcaca
     3781 aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg gcaagcattt
     3841 tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata ataatggtgg
     3901 agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag acttcccaac
     3961 agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag gaaaacctgt
     4021 agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc aattacctca
     4081 tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg aacaaggcga
     4141 cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg gtaatttacg
     4201 tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag cgatctttga
     4261 agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga tccaagtcgg
     4321 catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa aagaagtaga
     4381 tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag accgtatgaa
     4441 cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt tgatcaaaaa
     4501 tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag aaatggctgg
     4561 agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct caatgagtgc
     4621 gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga aaatggctga
     4681 acttgctgat cgtgcattga aagaatgcga tacgatggaa gaagtcgttg aacttgttca
     4741 tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag agctacgcca
     4801 gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa aaatagggct
     4861 taagtttgag tgacagtttg taggctatgt tatactatat aagtgaaggg ggacgtaagg
     4921 tgaaggtatt attatatttt gaaagtgaaa agatgcttgc aaaatctgga atcgggcgtg
     4981 cgcttgatca tcaaaaacgt gctctgacgg aagtcggtat ctcttataca ctagatgaaa
     5041 aagaagatta tgacatcctg cacatcaata catacggaat caatagtcat aatatggtca
     5101 ataaagccag aagagaagga aaaaaagttg tctatcatgc gcattctaca gaggaagatt
     5161 tccgaaattc ctttatcgga tcgaaccagc tttctcctat cgtaaaaaaa tatctggtag
     5221 gattgtacca aaaagcggac tatctgatca cgccaacacc ttattcaaaa cagttgttgg
     5281 aaagctatgg tatccgtgtg ccgattcaag ctatttccaa tgggatcgac ttagagaaat
     5341 atcaccctga tccaatgaaa gaacaaaagt tcagagaata tttcaaactg tctccggatc
     5401 aaaaagtaat catttgtgtc ggtttgtttt tcgaacgtaa aggaatcatt gatttcgtag
     5461 aaattgctaa gaagatgccc gaatacacat ttatttggtt tggtcatgtt cccatgtact
     5521 cgattcctcg gaacatccgg aaaattgtaa aagaagatca tcctgaaaat gtcctgtttc
     5581 ctggttacat tcgtggagag atcatagaag gtgcgtattc aggtgcagat ctattctttt
     5641 tcccttctta tgaagagaca gaagggatcg ttgttttaga agctttagca agcaaacaaa
     5701 acgtacttgt tcgagatatt ccagtgtata atggctggtt agaagatagc aagaactgct
     5761 atatggggaa gacaaacgaa gaatttatcc gactgatcca acagattacc aatcaagaac
     5821 taccgagtac gacacaagcc ggttatcaaa cagcaaaaga gcgaagtatc aataaaatcg
     5881 gtgaagaact taaaaatgta tacgaaagtg tactgaatga aaaagtctct agcaaattaa
     5941 aaaaagtaaa tcaggtcaag gactagtttg gaggcgaaaa acatgaaaat cggctttttt
     6001 accgatacct actttccgca agtcagcgga gttgctacat cgatcaaaac attaaaacaa
     6061 gaattagaaa gacatgggca tgaagtctat atttttacaa cgaccgatcc taatgcggat
     6121 aagttggaaa aagatgtgat tcgaatgcca agtgtaccat ttatttcttt caaggatcga
     6181 agagttgttg ttagaggcat gtggtatgct tatttggttg ccaaagaact agaactagat
     6241 cttattcata cccatacaga attcggagca gggatacttg ggaagatggt agctaagaag
     6301 ttgaaaatac ctctgatcca tacttaccat acgatgtatg aagactacct tcattatatc
     6361 ggtaaaggaa aagtgatcag gcaatcccat gtaaaatact tgtctagatt atttgcaaat
     6421 catacgacag gtgtagtttg tccaagtgaa cgtgttattg atacattacg cagctatggt
     6481 gtaatagcac cgcttcgcgt gattcctact ggtatcgaca ttgaaaaatt caaacgttct
     6541 gatatcactc agcaggatat cagcaaatta agggagtcac taggtcttca ggaaaaccat
     6601 ttgatgattc tttctttaag caggatttct tatgagaaaa atatccaagc cttgattaat
     6661 gggtttcctc aggtcatctc cgcttatcct aacgccagat tagttatcgt aggaaaagga
     6721 ccttatgtag aagagttaga ggaattgatc tcagaactgc agttagaaga atttgtgcag
     6781 ttcacaggag aagtggacaa caatgatgta gcactttatt ataaagcggc agattatttt
     6841 gtcagtgctt ctacttcaga aacacaaggg cttacttata cagaagcgat ggcagccgga
     6901 acaccttgtg tcgtggaagg aaatgcttat ttgaaccgat tgttcgatca tccttcatta
     6961 gggaaaactt ttgaaaccga cgaagacttt gcaccagcac ttattgatta tatttccagt
     7021 gaagttccta aagatccaaa agttttagaa gctaaacttt atgatatttc atctacacat
     7081 ttcggcgaag ctatgatcga attttatcaa gacatggttg tttatcatga agaagagatg
     7141 cgaagaaaag aagaagaaga ctcgattgaa cgaatcaaaa taaaattaaa ttcatttaaa
     7201 agataaaatt caaacatttt ttatgaaaaa atccttgttt ctagcgtata ctagcataga
     7261 agcaaggatt tttgattttt aggaggtcga aaaaatgaaa cttggtttta tcggaacagg
     7321 cgtcatggga agtgctgtcg cacgccatct actagaagca ggacacgaag tagctgtcta
     7381 taatcggaca aaagcaaaag ctgatccttt agtaacagaa ggagctatct gggcagatac
     7441 accaaaagcg gtagcagaac aaagtaatat tctctttaca atggtaggtt accccaaaga
     7501 tgtagaagaa atctactatg gacagtcggg gattttttct gcagatatct caggacatat
     7561 tttagttgat ttgactacaa gtacaccaag cctggcagaa aaaattgcaa aaacagcaaa
     7621 agaaaaaggc gcagatgcgt tagatgcacc ggtatccggt ggtgacttag gagcaaagaa
     7681 tggtacactg acgatcatgg tcggtggaga agaagccgtt tatgatcaag tactgccttt
     7741 attcaaagaa tttggtacga catttacatt gcatggttct gctgggaaag gtcagcatac
     7801 aaaaatggct aatcagatca tgattgcagg tacgatgaca ggaatgacgg aaatgcttgt
     7861 ctatgcacaa aaaaacggac ttgatttaaa gaaagtgatt gagacgcttt caggtggaag
     7921 tgcagctaac tggtctttaa gcaattattc tcctcgtatt ttaaaagaag attacacacc
     7981 tggttttttt gtcaaacatt ttatcaaaga tttaaagatt gcattagaag aagcagaaaa
     8041 aatggatctt gtattgccag caactaccca agcattgaaa ctatatgaag agttggcaga
     8101 taaaggtttt gagaatgacg gaacccaagc tttgatcaaa ctttggtggc ctgaaggaaa
     8161 aataccagaa aaaaaatcat aaaaaacgag tttttttaga gaaaatcaat gcaagtaggc
     8221 tatgcttttg atacaatata acagaaataa gaaaaggagg acctccgatg acacattcac
     8281 aattagtcgc aatcataaaa cgactggagg ctatggtcga gtcagcagat aatgaactgc
     8341 aagttcgccg ttttgaaaaa gaaggcgtag agaagtgtac agtaacttat gataaagcga
     8401 ccgatacatt tgaattaact gaaacagata ctcatcagca atacgaattt gataatatcg
     8461 atatcgtagc gatggaaatc tatgacttaa tccaataaat tttaggataa ttgacatttg
     8521 aagttgaaga tgcctaaaag aagccgagaa gcaactaatt tagttgcttt tcagcttctt
     8581 ttttgtttaa cagttcaatt tcaaactgtt ctttttctaa ttttttgcgg taactgatca
     8641 agatttgtac aggatcgatt tcttcttttg acaattgacg tttcaaagat aaatgagctt
     8701 ctttcttttc ataatagaag tcaggagaaa cgtcaccaga ggtaatcatt gtttctgcaa
     8761 gttgaccata acaaacgagg tagtgctttt tctgactctc atctgctgct aatggcatgc
     8821 tgaaggtgag cgatggtgtt cttaaaaact gcttttgttc ctggatccgt ctaataaaat
     8881 aagtaactaa ttcgatatct ttttgaagca taccggcttt ttcgaaattc aactgataga
     8941 tgaaatgttg ttgtcttttt ttcaagtaag tcagtagctt tttatttcgg ccttgaaaca
     9001 gcccttggag ttcctttttt ttctgttcaa aagtcatttc ctgtaggtca ggaagctgga
     9061 ctcgcaaagc taacaaacta atttcagaca accaaggcat ttgataagtt tcttccatta
     9121 tacgcaaaat agacggcatt tttttataga gacgaaaagg cccgtatgtt cgagccgtag
     9181 gagtatctgt taatacaaat cccttattgg taatatgtac ataattataa ttactgaaat
     9241 aattcatttg tcggttataa aaaggacggt aatgttggat caattgacac tcgagcaata
     9301 aagcatctaa ttccgtgtct actacgacga aatcaaaatc tgttatatga tgaatcatac
     9361 gtaatacttt ttttgaatgc tgtttattct gatgaaaata gctgcttact ctatttttga
     9421 gccgttttgc tttaccaaca tagatgacct taccatctcg gtctttcata agatagacac
     9481 ccggcagcat ggggagagtt ttcatttttt cttttaacat aggatctcct tttacgcttt
     9541 gaataaaaag actataacaa attttgaaat aatggacaag ttttagggaa ttctatagtt
     9601 ctgttcacag aaaatacttt gataatcaaa atatgtgaga tagatgattt ttctaataaa
     9661 agaaattata aatgctgatt tatcagttgt tttaaatgag taaa
//
LOCUS       DACENJ010000094         9689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.095, whole genome shotgun sequence.
ACCESSION   DACENJ010000094 DACENJ010000000
VERSION     DACENJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.095"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(285..3434)
                     /locus_tag="IW748_001638"
     CDS             complement(285..3434)
                     /locus_tag="IW748_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1104551.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYNTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3448..4692)
                     /locus_tag="IW748_001639"
     CDS             complement(3448..4692)
                     /locus_tag="IW748_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1104552.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(4685..6277)
                     /locus_tag="IW748_001640"
     CDS             complement(4685..6277)
                     /locus_tag="IW748_001640"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1104553.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(6786..7511)
                     /locus_tag="IW748_001641"
     CDS             complement(6786..7511)
                     /locus_tag="IW748_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104554.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(7665..7991)
                     /locus_tag="IW748_001642"
     CDS             complement(7665..7991)
                     /locus_tag="IW748_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104555.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8028..8393)
                     /locus_tag="IW748_001643"
     CDS             complement(8028..8393)
                     /locus_tag="IW748_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1104556.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(8430..8645)
                     /locus_tag="IW748_001644"
     CDS             complement(8430..8645)
                     /locus_tag="IW748_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1104557.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            8774..9109
                     /locus_tag="IW748_001645"
     CDS             8774..9109
                     /locus_tag="IW748_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1104558.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9185..>9689)
                     /locus_tag="IW748_001646"
     CDS             complement(9185..>9689)
                     /locus_tag="IW748_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104559.1"
                     /translation="FTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSKEKQLILDEAH
                     EVLQGLSWQGTYLMTEKELINTVCERLRNVRDNSVKNALIENRGYFVKQGFTLERASK
                     DIKKLYNVKASSSFLVYLAKGAGIDEVIKELILSGVELVPIYQFTSVTRDDGRRVRNR
                     RDPIILN"
BASE COUNT         2793 a         1974 c         1490 g         3432 t
ORIGIN      
        1 atctccgttt catttcagat acatttcact taagcggatt ccaaaaaaac aaaagaaaaa
       61 ggaaccccta gttagcctat gctttttatt ggttcctctc aaaacacggt catataacaa
      121 cggggtgggg taaatgaatg aaaaaggtgg aggaaacatt attcaatagc tgtcttaaat
      181 acttcgttat aaaatgtgaa caaataaaag acatctatcc ctattttcta cacttccact
      241 ttactaccat ctttgaatcg tacaattttc ttaactcttc tcgtttacct caattcattt
      301 tcaagtggaa tcagctcatc atctaacatt ttacgccatg ctcgcttcat ttcttgtggg
      361 tatttaaatg gcttcgcttc tggatggttg gctttatatc cttcgtaatc cttactgttg
      421 ataattgctt tcatattagg aattggatct tcacctggca tatattgcag attagaagca
      481 tgtaattctt tctcagaaac aaaccactca ttggaaaaga catgaatcaa atcatcactc
      541 gcttgtgtaa aatagcgacg tttaatgaca aagatatctt cattactatc taaagtcccc
      601 acatcaaacg catttaaaat ttgtcggtaa acttctttga ctccttctgt tttattcaat
      661 ttagccaaag ctttttcaat ttcttctcgt gcatctgaac tattcgccgc ataagttcct
      721 agtaattgat caatataagt agaatcaata tcatacaact tcgtggtatt ttcactataa
      781 aattcgatcg tggaaaaatc ttctccctct ttttctggtt cttcctctat taatgaaccc
      841 ttcacggtct cgtacacgcc aatttgttct tccaaaagtt gaatttgatt gttcaaggtt
      901 gaagatttca ccatgtcatc attgtaatcg tcataagtca ctaacgattc ataagcacga
      961 ttcaattctt ggaatgcttt gacgtactcg atacggattt caagtggcgt atgttcatca
     1021 atggattcca tggatgaaac ctcttttaac ttaccatatg cttgcttgaa tctttttttg
     1081 gattcctgat aacttggata aatcaaaccc gattcttctt ttgcttctga atacagtttt
     1141 gtcgcatcct cgacattctt ctccattgtg gctggctttc ggaaagtaac aattagtcct
     1201 tttgtttttt ctggatacgt acggttcgta cgtgaaaaag cttggatcaa tcctgcatac
     1261 tctagattac gatccacaaa tagggtttga atcgtaggtg cgtcaaaacc tgtcaataga
     1321 cgatccacta cgatcactaa atcaatttgt ttcccaaatt gtttgaattc cgctcttttt
     1381 cgtgccaaac gattattgat gtctccgtta taacgctcaa tgtctgctaa tgaccaggaa
     1441 gtattataat actgattata ttcctcaata attttttgca tttcttcttg ttggacactt
     1501 gaatctcggc tgttttcttc taatgaatag gtaatagcga ttcgtggaaa atcaggatcc
     1561 tccatcgttc ttccttcacg aatgggctga ttaggaaact cttttgttaa ccagtcaggt
     1621 tctttcgtca tttctttgat cgctcgataa tactttttgg ccatgtcaat ggaactcgtg
     1681 gttaagatcg cagacttcgt tggacggcca tttcgaaaat caaattttgt ataggcattg
     1741 tctggacgga acattttatg aatgactttt tggatatgtt catctgcttc aaaaacagta
     1801 gggtccaaat aactttcttt tttgacaggc tccatttgat caatcatacg attgatctgt
     1861 tccggtgaat actctgcata tgtttctact tcttttaatt tccgatagat tttattctct
     1921 aatgaagtcg gctccacggt gttttcatgc tctacttgaa atcctaaaac cgagccatct
     1981 tctaatgcat tcttgatggt ataggtatgc aagacctctc catactgatc atgggtcgta
     2041 cgtgccaatt gccctttggc ttgtttccga ttctcaggga aaatcggtgt acctgtaaag
     2101 ccaaaccaag tggatttcgg aaacattttt ttgatttctt ccatattttc agcacttaac
     2161 gctcgatgac actcatctac tacaaacacg atatgttgcc ccattaattt ttggaagcga
     2221 ttggttcctt ttttctcttc ttgttttttt gcatatttca acgccgcatc tagcttttga
     2281 cgggttgtaa taatcacaac atttgcattg gcatctgcca acagagtttc acttaactct
     2341 ttggcacttc ctgttcccac gatcaagcta ttcgctttgg cgttcccgga agaaatccca
     2401 gtattaaatt ccgaagcaaa tttcgtaaac tctgtggtgg tttgattatc taaatctttg
     2461 cggtccacta acatgatcgt ccgatcgatc cccgattttc gtgctaaaag cttagtagat
     2521 acaaaactag tcaacgtttt tcctgaacca gtcgcatgcc acacatatcc tgattgatgt
     2581 ttatttgcag caataaacaa ggcttgaatc gcatgtactt gataaggatg taacaccatg
     2641 aggattttat tgtcttgatc ttcactgaca atcgtataat ccgcaatcaa gcgatgagca
     2701 tccgggatat ttagcacttg tttgcaaaac tcatataagt tttctacctt tttattatct
     2761 ttcgttcgcc aactaaataa aaacttagga tgcaaatcct ttggtaaggc atttgcaaaa
     2821 taccgagtgg tttgttcatt ggaaaccaca aacaactgta aggtcgaaaa gatattgttc
     2881 cgaaacatac cttcttctgc atattttttg atttggttaa acgcctgata aaaactatct
     2941 tttgcggtta cttgtttcag ctcgatttgg acaatcggta atccattgat cagtaaggtg
     3001 acgtcaaaac gtcgatcccg tgcttctatg ttacttccac gttttgcaat ttgatggacc
     3061 acttcatagg tagagattcc tccaccaatg tcttgattag aataaagaat gagcgagaca
     3121 gaacccagtt gcggatcttc ccgttcaatc gtgatccgag ccattccatt ttcacctttt
     3181 aaccatttcg cagcatcaaa gggggttttc gttcgtagta acaattctgt tttgatcgtt
     3241 tcaaattctt tatcggtcaa aggaaaatca ttcagttctg cttgattatt tgagatgatt
     3301 ttttggcgta aattgaccca aagatcttct tctgatttta gatcgggacg atacgtccac
     3361 tgattatgcc cttcccccaa tacacggatc aactgttctt cgacttccgc ttcctcacgg
     3421 tgactaattt tcatttatct tccctcccta aacaaacatc ttttgtaaga agcctttttt
     3481 cgtttccttc agcaaatcta acttacgttg atgaagagcg atcgtgtcgt cgagttgttt
     3541 gaagaaagcg ccgatttttt gttgttcctc tttattccta gtaaaaggaa acttgatatt
     3601 agcgaaattc tcatagaata agtattctcg tacgcttcct tcagtgttgc gcttgacctc
     3661 tttgataaat gttggtgact tgataaactg aagtatgaac tcattatcta agtcctcgct
     3721 cattttcaca actacataaa gagagctaac aatgaccgtc ataccaagat tgttaaatgc
     3781 aattgatcct acgtttactc ttgctggatt ataggcgaat tcatttggtt caacgaattt
     3841 ataatttgtt ttttctaaat catccaacct actaccatca aactgttctg tttgagaaat
     3901 taaacctgct ttattactta ccgaataggc agggaagtct aaatctttcg tgttcttttt
     3961 tccagtcttc ttactaaact ctttgaactt acgctgttcc caatcttccg taaatccagg
     4021 aaaccgaatt tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt
     4081 tttcgtttcc tttaataaat ctagcttacg ttgttgaaga gcgatagtgt cgtccagttg
     4141 tttgaagaag ttcccaattt tcttttgttc ttctaatatt ggaaattgta ctatccattt
     4201 tttcacttct gacattggta cgagatttgt aattgctgaa cctggattag ctcctgtgag
     4261 ttttctttgc ataaaatctg cacgaataaa ttgaataaaa tattctgaat ctgtgatttc
     4321 tttatttatt ctaagtctaa tcaaagcctg attgaataca cccggtttta tttgctttgg
     4381 tactcttgaa attctcccaa ttgttccagc gccactcatg ataaaatcgc ctggttctaa
     4441 tttaaatttg attaattcat tatatttttt ttcagtaatt ttatatcttg tttcgtaatt
     4501 atcataaata gcattttgtt gttcatagac aacaaaattg ctattagata caaagaattc
     4561 tttctttaag gcactcccaa atggtcccct ccttactcca tcttttttat caagtaagca
     4621 ttcaaactta cgctgttccc aagcttccgt aaatcctggg aaacgaattt ctggttgtat
     4681 atcattactc atgtttgaac acctccagcg caccttgtaa ccacgcttca ttttcaggtg
     4741 ttgtttgcag ggaagagata gcttctaaaa gttctttttc tagcacttgc tgttcttgtc
     4801 gtatcttctt gatgtcattt ccgacatgaa ccatatcaat tggttcttct tcttcaaacg
     4861 tatctacata gcgtggaata tttaagttat agtcattttc tttgatttca tcaaagtttg
     4921 ccaaatgagc gtatttctcg acatcttttc tttcagcata ggtttcgagg attttttgaa
     4981 tgttttcttc cgataattta ttctgatttt ttcctttgac aaattctcga ctagcgtcaa
     5041 tgaataatac gtctcgagtt tggcggttct ttttcaatac aatcaccgtc gttggaatcg
     5101 atgtcccgaa gaacaagttc gcaggcatac cgatcacagc ataaatactg ccgtcttcta
     5161 gtaatttttg acgaatgacc ccttctgcag cgccacggaa taaaactccg tgtggcaaga
     5221 cgatggccat agtccctgtt tccttcaaat gatagaaacc atgaagaaga aacgcaaaat
     5281 ctgctttgga ttttggcgct aattttccat aacgattaaa ccgagaatcg tctaagaagg
     5341 tcgtatctgc tgaccaatta gccgaataag gtgggttcat tactactgca tcaaatgtgt
     5401 aaggttcatc tgttggccaa tccttattca acgtatcccc attgcggaga ttcatttctt
     5461 cggcatctac cccatgtaaa atcaagttca tttttgctag attgtatgtc gttgtattta
     5521 attcttgccc atgatattta acgttatctg ggtgagttaa gtagttacgc acatttaaca
     5581 tcaatgaacc tgaacccatg gttggatcaa aaacactaaa gaatcgtcgt tctttttgat
     5641 ccaacgtcac gatttgtgcc atcatatcag aaaccatatg aggcgtgtaa aattctccgg
     5701 ctttcttccc tgcttctgaa gcaaattgac tgatcagaaa ttcataagca tccccgatca
     5761 cgtccccatc gtgacctaaa acttccacgt cattcaattt cttaatgact tcggtaatcg
     5821 tcacatttcg ttgttgctca tctgttccta gtttttttga ttggagatcc acatcatcaa
     5881 ataacccatt aaattgattg taggtagatg ccaattgcac aaaggctttg tttagatcat
     5941 tcaattggaa cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga tactctggcg
     6001 aaatcgcata ccccaaaata tcaacgattg ttgcaatcag atcttctttt gattcttcat
     6061 ctgaaagtaa ttcgcgatac aacatcgttt gtttgcttac cgtatcatat tcctctaagg
     6121 attcatctgc taaaagaacc acttgttcca acaaacggtc ggataaatat ttatagaaaa
     6181 tcagtcccaa taaataattt ttatattctg acgcatccat tttgctacgg agattatctg
     6241 ccgcactaaa taatttttga tttaattctg cacccaaaaa gatacactcc tatctcttat
     6301 ttaaagttgc tatataccct cttattttag catactccct tttagaaaaa gcaaaaaaaa
     6361 aagaaagaaa cttattcttg ctaataaact acgcgaaagg agtctaacgt tgtttgatat
     6421 aacttagact gatcgttaac ttatttttta tatttctaca tagttatctt tagaaccatt
     6481 tcagatacat atgaaacaat tacatatgat tttttcataa aaaaacagta ctgtctagta
     6541 gtataaaata tacaaatttc ttaaaaattt tttcactaaa atcactacct gaaacagtct
     6601 aaaacactta tatatcaacg tttaatgaaa ctatctccgt ttcatttcag atacaaataa
     6661 atatagtcgg gctccaaatc cgaaaaaaaa tacccccagt agcttatcct aagctactgg
     6721 agatgtataa gtacggtcat ataacaacgg ggtagggtaa ataaatgaaa aagatggaag
     6781 aatcattatt caataacttt tttaaatact tcgttgtaat cagttacctg tccgttctta
     6841 aataccacag aataatcttt tttcccattt tcaagtaacc gaagtggaat atctttacca
     6901 actttctctg tcactgcatt actgatacct gttagattag ctaataaatc tgataaagat
     6961 tgagcatctg tagaaatctc tgtatcagta tttatcgtga ttcctttatc ttcatttaca
     7021 agtttccaag taggtggtat agctgtgtat gtttctacaa atgaattaag tttttcatta
     7081 agtgacttct cttttatatt tccatctaaa aactctgtat atatggcttg agctatacta
     7141 aaacaaagct ggccttgttc taataatttt acatcattag ttagtaaagc tgtgttcacg
     7201 ttatcaatcg cttcaagaag agtatctttg gtgtccctaa cagctttagg tgtgccttct
     7261 ggaatttccc tacttgatac ctcgtccatt ttatcttcca taaacccctt aaaatcatat
     7321 ctttctccca aatccataga agtttttgag aaagctttca tttctgagtc tatttcttca
     7381 ataatattta acatgtctga tacatattgt ttaggctgat tcgtttttaa tctatatgtg
     7441 gtaaatatct caattcctcc aattatcaat aaaattgcca aacaaatcca taataatttt
     7501 ttctttttca tttttcctcc tatacactgc tgttataaca gtgactaatg taaagatttt
     7561 cccagcaatc atcagactat tatttacagg gaatcaaaaa aactccttag taagcttaca
     7621 cctttaggag ttcctgtttg aacggtatgc ataatacatt taaatcaatt taacattaac
     7681 acctcagtat atggtatctc tacatataca cggtcttttt ttggatagta ctcgacattt
     7741 atattgatag cacttccatt gtctaacgta gctttagccc ggtacgtgtt atcaccatta
     7801 tcatgagtga tcgtcacatc atcacatgtc cgatttaagt tatactgttc ttgtaaaatc
     7861 tgagttacca atggtacaga tgacttctct agtccagatg aacccgtcat tcttgaaaaa
     7921 attataccta taatagccaa tacaacgata cctataatga caaacttctt attcatattt
     7981 actttattca actttttatc tcctatctcc aaaatgattt tctctcatca gtaactcatt
     8041 gacatcccca atagtacacc aaagatggct ccagctaccc ctgctatcat caaaatcgta
     8101 cccatttgtt catctttctc tcgatataag taacccataa taacagcaac agcaccaaat
     8161 accacaggaa taaatgctaa tgatactacc atagaaatcc aaccaataat caaatacagc
     8221 ttccctgtct cttgtccatg tacgatttgt tccttcattt cattaattgt ctgctcagta
     8281 cttttcttta ctgtgtctgt tccggttgtc tcatctgttt cttgtaatgc tccgcaaaac
     8341 tggcaatact tactttcatt tgttatttct ttaccacatt ttctacaata catgtcttct
     8401 cctcctaaaa tcattcaatt gcttattagc taactgttta ctttattaaa tacatcagaa
     8461 aacagctcat ttattggtac acttaatatc tcagaaagca ttttcatttg atagggttta
     8521 ggaatcgttc tccctgtttg ccacgaactt acacattgct gtgtaacatt catttttttg
     8581 gctaactcag tttgggaaag tcctttttct tttctgtatc ggctgagatt acttactttt
     8641 ttcatgattc tcccctcctg ttttattata tacaacaata tgatgtattt caactcataa
     8701 aatatataaa tacttcaaat gtcattacaa atacaacaac ttgttgtact ctactgagaa
     8761 ggggggtgta ttaatgaaac agaagcaata tgcaaaggtc attagagaaa agcgaaaagc
     8821 actcgggtgg acacaaaaag aattagctaa aaaaatcttc tctacacaac aggcagtagc
     8881 aagatgggaa aattctgtaa cagaacctaa cctcgatagc ttaacagctc tttctagagc
     8941 cctaggaacc cctgtaagcc attttctaga caatgttgac atagattatg aggaagagtt
     9001 tttggctctc tatcgctctc ttagtactga agatgcaatt cgaacaattg actatatgaa
     9061 gctattaaaa aggcaagaaa atgaacgcaa tcagctattg aaagattgaa aaataaataa
     9121 cccaccgaac tttaacggtg ggtgtattga gatgaaataa gattaagagc tatggataac
     9181 ctgtctagtt taatataatt gggtctcttc tattccttac tctccgtcca tcatctcttg
     9241 ttacacttgt aaattgataa ataggaacta attcaactcc tgataatatc aattctttta
     9301 ttacttcatc tatccctgcc cctttcgcta gatatactaa aaaactactg cttgctttca
     9361 cattgtatag tttcttaata tccttagaag ctcgttcaag agtgaatcct tgtttaacaa
     9421 agtagcctct gttttctatt aaagcatttt tcacgctgtt atccctgaca tttcgtaggc
     9481 gttcacaaac agtgttaata agttcttttt ctgtcattag atatgtccct tgccacgata
     9541 agccctgtaa aacttcatgt gcttcatcta aaatcaattg tttttccttg ctaagttctg
     9601 atcgggaatt ttgaggaaat actcttttag cctctttttc gttaaaacat atcttaaccc
     9661 attcataaga aaaatattga tatgtaaaa
//
LOCUS       DACENJ010000095         9678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.096, whole genome shotgun sequence.
ACCESSION   DACENJ010000095 DACENJ010000000
VERSION     DACENJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.096"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(777..2408)
                     /locus_tag="IW748_001647"
     CDS             complement(777..2408)
                     /locus_tag="IW748_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1104560.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2422..4095)
                     /locus_tag="IW748_001648"
     CDS             complement(2422..4095)
                     /locus_tag="IW748_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1104561.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4108..4425)
                     /locus_tag="IW748_001649"
     CDS             complement(4108..4425)
                     /locus_tag="IW748_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1104562.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="IW748_001650"
     CDS             complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="IW748_001650"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1104563.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6087..6872
                     /locus_tag="IW748_001651"
     CDS             6087..6872
                     /locus_tag="IW748_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1104564.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6875..7567
                     /locus_tag="IW748_001652"
     CDS             6875..7567
                     /locus_tag="IW748_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1104565.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7841..8212)
                     /locus_tag="IW748_001653"
     CDS             complement(7841..8212)
                     /locus_tag="IW748_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1104566.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(8612..8842)
                     /locus_tag="IW748_001654"
     CDS             complement(8612..8842)
                     /locus_tag="IW748_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104567.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(8936..9274)
                     /locus_tag="IW748_001655"
     CDS             complement(8936..9274)
                     /locus_tag="IW748_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104568.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT         3005 a         2073 c         1574 g         3026 t
ORIGIN      
        1 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
       61 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
      121 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
      181 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
      241 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
      301 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
      361 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
      421 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
      481 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
      541 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
      601 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
      661 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
      721 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
      781 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
      841 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
      901 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
      961 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     1021 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     1081 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     1141 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     1201 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     1261 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     1321 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     1381 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     1441 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     1501 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     1561 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     1621 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     1681 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     1741 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     1801 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     1861 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     1921 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     1981 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     2041 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     2101 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     2161 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     2221 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     2281 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     2341 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     2401 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     2461 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     2521 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     2581 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     2641 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     2701 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     2761 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     2821 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     2881 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     2941 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     3001 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     3061 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     3121 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     3181 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     3241 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     3301 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     3361 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     3421 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     3481 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     3541 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     3601 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     3661 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     3721 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     3781 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     3841 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     3901 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     3961 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     4021 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     4081 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     4141 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     4201 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     4261 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     4321 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     4381 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     4441 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     4501 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     4561 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     4621 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     4681 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     4741 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     4801 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     4861 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     4921 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     4981 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     5041 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     5101 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     5161 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     5221 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     5281 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     5341 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     5401 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     5461 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     5521 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     5581 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     5641 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     5701 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     5761 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     5821 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     5881 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     5941 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     6001 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     6061 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     6121 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     6181 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     6241 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     6301 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     6361 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     6421 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     6481 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     6541 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     6601 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     6661 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     6721 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     6781 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     6841 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     6901 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     6961 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     7021 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     7081 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     7141 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     7201 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     7261 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     7321 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     7381 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     7441 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     7501 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     7561 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     7621 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     7681 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     7741 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     7801 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     7861 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     7921 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     7981 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     8041 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     8101 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     8161 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     8221 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     8281 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caacccgttc
     8341 tctgagtgtt gattctataa tatttttaat ttcctatata aatcaaaaat attatagaat
     8401 caataagcta attaagaaac aaactttaga aactctagaa gaacttctga atttatatag
     8461 aagtgtgtag ctacttacta aatatacaaa agttaaaata aaatactctg gatactcttt
     8521 atactgcatt aaagttagta atattaacag caaagatatc gaaaaaacaa taacataata
     8581 tgtgtcaaaa tttttcataa taagaactcc cttactgttg catactaaca taatccaaaa
     8641 actcttctac ttcagctaaa gacattccac tttctttagt ccaaaaaaca gttttttcta
     8701 tcgatccttc tctgtaaact gtaaatgtcg gagtcgagtt aattgagtag cgactcttga
     8761 attctttcca ctcattttca tcttctctaa cttttgctac attcacgtaa taaatatttt
     8821 cttttccctt ttctaccagc atatcgaata gcttcctgct tttgatgatc gatttcttgt
     8881 gctacatcta gtaaattttc atcaatttct atatcataaa gtatttcact agtgtctaca
     8941 tccacaatat atacttttta tcagtagcaa tttttgagta atccgttaaa atgatctcag
     9001 tctcgtcact ttcatcagta taaatatacg ttaacaatga actcaccttt tcaatcggtg
     9061 ttagctctaa gcctactgta ttttttacta ttgagtcatt atttaccacc ataacttcgt
     9121 actgtatata ccatcctatt tggtttccat caatatcagt aattggctca ctagctaatg
     9181 gagtctccgg aacaatattt tcctgatatt tcttaaactc ttctgtagca cgaaaaaggt
     9241 tatcatgttc ctcacttgaa actggttgag tcatagaaaa tgttacttgg ctaaacagag
     9301 ggaaaataaa cactacaatc aacaaagtat aaaaaaaagc ccttcttttt cattttttat
     9361 cttccaatca caaattgaaa aacttattcc tgcaactttg agtctagtat cacaatcgac
     9421 tgactacaac tattcattta cattctactt taagttcctc cttaaccatt aattttatta
     9481 aagatacttg taatagtgat tctcacaaaa tttcatgaaa aatactttat tactgtatct
     9541 aaaattccaa tacacaactt atcgactatt atctgaaaac caaaacttat tattccttga
     9601 tgttattcta gcaatagtaa ctgctttttt atttttttgc atattttttc ggcgttactt
     9661 tttaaagtta actttttc
//
LOCUS       DACENJ010000096         9655 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.097, whole genome shotgun sequence.
ACCESSION   DACENJ010000096 DACENJ010000000
VERSION     DACENJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.097"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(167..796)
                     /gene="udk"
                     /locus_tag="IW748_001656"
     CDS             complement(167..796)
                     /gene="udk"
                     /locus_tag="IW748_001656"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1104569.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(880..1575)
                     /locus_tag="IW748_001657"
     CDS             complement(880..1575)
                     /locus_tag="IW748_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1104570.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1588..2304)
                     /locus_tag="IW748_001658"
     CDS             complement(1588..2304)
                     /locus_tag="IW748_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1104571.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2634..3239
                     /locus_tag="IW748_001659"
     CDS             2634..3239
                     /locus_tag="IW748_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1104572.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3564..3887
                     /locus_tag="IW748_001660"
     CDS             3564..3887
                     /locus_tag="IW748_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1104573.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4069..5439
                     /locus_tag="IW748_001661"
     CDS             4069..5439
                     /locus_tag="IW748_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1104574.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5569..6870)
                     /locus_tag="IW748_001662"
     CDS             complement(5569..6870)
                     /locus_tag="IW748_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1104575.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7100..9091)
                     /locus_tag="IW748_001663"
     CDS             complement(7100..9091)
                     /locus_tag="IW748_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1104576.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9235..9501)
                     /locus_tag="IW748_001664"
     CDS             complement(9235..9501)
                     /locus_tag="IW748_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1104577.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2821 a         2004 c         1753 g         3077 t
ORIGIN      
        1 aaaatataaa cagctatttt ccgtttcttc tttttaatac ttaggtcaag atgctgtttt
       61 cacaacctct tgttataaat aaaaaaacag ttggctggtg actgttcaaa aagcagaatc
      121 cttgcttttc aaacggtcgc agtccgttca actgtttcgc tgcgcactat ttatggttta
      181 agaaactagc tacttttgtt gtaatcaaat caatagcaac gtggttttct ccgccttctg
      241 gaacaatgat atccgcatag cgcttcgttg gttcgataaa ttgatgatac atcggtttaa
      301 caactgtcaa atattgttcg atcacggagt ccaatgtgcg tccccgttct tccatatccc
      361 gtttgatacg acgaatgata cgaatgtcat catctgtatc tacgtatact ttgatatcca
      421 tcaagtcacg cagacgttcg tcttctaaaa tcaggattcc ttctagaatg atcacttctt
      481 ttggttcctg aatgatcgtt gcctgacttc ttgtatgcgc tacatagtca tacacgggtt
      541 tttcaatggc tttatagttc agtaagtcgc ctacatgctg aatcagcaga tcattatcaa
      601 aggcaaatgg gtgatcgtaa tttgtgttca atcgttcttc aaaactcaaa tggctctgat
      661 ccttgtaata agaatcttgt tcaagcatca tgatggaatg gtccgggaag ttgttgaaaa
      721 tcgcacggct gacacttgtc tttccgcttc cggatccacc cgtcacacca atgataattg
      781 gcttgctttt tgtcattgac taaaacctct ttcgtatccg ttttttcatt ctaatctatt
      841 atagcgaaaa aacacgaaaa tgctatattt ttctgaaaat taatcctttt caatttccaa
      901 taatgaataa agttcctcta atgaatcgat tcgataagta ggctggattt cttcgatttc
      961 tggtaattta tttctattga accaaatcgt atcgatccct gccatatttc ctcctttgat
     1021 atcagaagtc aaagaatcac caatgatcac agtttgcttt ttatcaaaat taggaatgtg
     1081 ggaaaagaca tgatcaaaaa attctttcat cggcttttgc gctccgacag cttctgagac
     1141 aaagatatcc ttgaagtaat caagcattcc tgcatctgtt aatcgccgat gttgcgtgtg
     1201 cgacacaccg ttcgtcacaa tataaagatc agcatgcgga tggacctttt tcaggatttc
     1261 tagacttcct ggaacaagat catgaccttg gttcaagtag tggcggtagc gtttttccat
     1321 ttgatcacta tctactgttt tcccaaattg ctggaaaagt aacccaaaac gactgcctat
     1381 tatttcttct cgtgatagtc tgcctttttc aaactcctgc cataaatgat gattcaaagc
     1441 cttatagctg gtttcgattt ctggtgtcaa tgtgattcct tgttctgcaa acaaagcact
     1501 taaagcattg gcttcagtta attgaaaatc taacaatgta tcatctacat cgaacaataa
     1561 agttttatat ttcatctttt tgacctccta tttaaaataa atacttaact ttttcatctc
     1621 tgctaatgtc aacgatcgat actcgcctac tcctagctgt tcatctaatt ccaatggtcc
     1681 catgcttagc cgtttcaatg cggtcacttt ctttccttgg gctaagaaca tctttttgac
     1741 ttgatggaat tttccttctt ggatcgtcaa taaaaccgta ctttggtgct tgtctgcttt
     1801 gagtattttt aaatcagccg gcttacattg atacccatca gcgaatacga ttccattttc
     1861 aaatgctgtt acgtctgccg gtgtcaccct ttcgttcaca actacttgat aagtctttga
     1921 aacctttttt tctggaagta gcatttcata tcccaattgt ccattcgaag tgatcagcaa
     1981 tagtcctgtt gtatctctat ctagtcttcc cactggatat aatggttgga aacgatcggc
     2041 acttgcaatc agatcgatta ctgtcgtctt ttctggatct gatacagctg taacagcttt
     2101 ttcaggttta ttgagaagat aataggactc atttgtttct aagtgttcac ctgctacttc
     2161 gatttgatgc agtaaactat ctacatttcg attttgtcga aattcgactg tgccatctat
     2221 catcacttgc ttcaaagcaa acagccgctt catttccttg cgtgtcgttt ctaatttttc
     2281 ttctattaat ttatctaaac gcatgctgtt tcctttctta ttactactaa ttaattatac
     2341 cttgaatatt cttgcttgca aatacatcat catagttatt ccttgactta attaacagtt
     2401 cacgttacgg ttaaatagta aagtgaattg gatttctcat acacagcgta atgacctggt
     2461 ggagaaaagt tgaatcctga ctttctaagg ggagatgcgc gcatctctcc ttttgcgctt
     2521 ttttaagcgc cctgccttcg atgatgtgca cgagattagc ctttagaatt cacttaaata
     2581 aaatctgtcc gttaccaaag cagacagtca cattttaagg aggaattttg gttatgtcag
     2641 tatcattaga agtaagtaaa agagaagtac gtccccgttc attaagaaac aaacttcgtc
     2701 acgaagggaa agttccagct gttgtttatg ggtataaagt agaaagtacc cctatctcat
     2761 tcgatggttt gacactaagc aaattgttgc gtgaacatgg agcgaacaca gtcatcacga
     2821 tggacatcga tggacaaaaa gtcaatacat tggttcacaa gatccaaacg aacactttta
     2881 ccaatcattt cgaacatgca gaatttctat ctgtaaacat gtcggaagaa acagaagtcg
     2941 aaacagaagt cgttcttgtt ggcgaatcag tgggtgctaa atctggtgga gtgttagctc
     3001 aaaacttgta tacagtagtc gtatctgcta caccagacaa attgcctgaa cgaattgaag
     3061 ttgacgtgac aaacttggaa ttaggtgatt ctattacagt agccgatctt ccaaaaaacg
     3121 acgattacaa attcgtgacc gacagtgaag agcaaatcgc tgctgtagtc gctcaaagtg
     3181 aagaagaaag cacaggtgaa ggtggcgaac cagaagtgat cggttcaaca gaagaataat
     3241 actgaaacaa tcaggtggtg ttagaagcgg cgggtcagca gcttttaaca cgcctttttt
     3301 atttctaatc gtttattttc atatggggct tccacttttg taaatggctt tacaaacgaa
     3361 aaatcctaat ttaccaagct tttcctcgca tacttttatc gtacctttat aaaaatttat
     3421 actttgaaaa aaagtattca attattgttg catttttcct gattctttat tagaatgaac
     3481 cctcgttgaa gtaagtattt ctagtttatc ctaggattaa ttataaaaat aaaacattcg
     3541 ttgattcgaa ggaggttaaa cagatgtttg aaagttttga tagcagccgc aaccgttacg
     3601 cttcagttgg tgtcgtatca agtttgccag atgaattgat tgacagtata tggtttatta
     3661 ttgacctaga tcttaaggga gtcattccat tagaaaatat attaaccttt gatttgatcg
     3721 aaaatcgtgg aaaagtgacg atgcattttt ctcaagcagg aagcaatgtg gaaatggata
     3781 ttgatttgcc gtttgcctat tcgaaccaat ttcctgctca agtatacgcc tatgatgacg
     3841 gtacgagaga aacgatcctt ttaccaagtg agattagaca atattaaggt gatagataaa
     3901 cgtggatgtc ccttatttta cagaccaggc caagttgttg attctttgac tcaattcttg
     3961 gcttcttttt atggagtttc ataaattatc attgatttct aattggtatt ctcaggataa
     4021 acgctatata ctactaacta aattttaaag atataaggag tttaaaagat gcgcgattta
     4081 acaaaaggaa gccccgctaa actgattttg atgttcactg ttcctttact gatcggaaat
     4141 gtttttcagc agttctacaa tatgatcgat atgatcatcg tgggtcagac gctaggtaaa
     4201 aatgcgctgg cagcggttgg tgcaactgga agcctgacat ttttgatcat tggctttgcc
     4261 caaggtttaa cagctggttt agccattatt accgcacaac gttacggtgc aaaagattat
     4321 cggggtctga agaaaagttt tgctgctagt gttgtcatta gtcttgtcgt cactgtgatt
     4381 ttaactgtcc taagtctcct gtttattcgt ccgatgcttc aattgatgca aactcctcct
     4441 gaaatacttg atcaagcaca aacatttatt tcaattattt tattagggat ctttgcatca
     4501 gtcagtttca atttgctttc taatgtcatt cgcgcacttg gtgacagccg cacaccacta
     4561 ttctttctga tcattgcggt catcatcaac gtcgtacttg atttgatctt tattatctat
     4621 tttggcatgg gtgtggaagg tgcagctatt gctacagtaa tcgcacaagt ttcttccagc
     4681 gtactgtgtc tagtgtatat caagaaaaaa atgcctttgt tacagttacg caaaaaagat
     4741 ttcaaatttg aaaaagaaga gtttgctgta cacctaaatg ctgctttgcc aatggcgttt
     4801 caatcttcga tcatcgctgt tggtgctatc gtgttacaag cagcactaaa tagtctaggg
     4861 acagatgtcg tagctgctca agccgctgct ggtcgtatcg accaatttgc aaatcagccg
     4921 atgatgtcat ttggtattgc aatggcaact ttcactgcac aaaactatgg tgcgaaagaa
     4981 tatggccgaa tcttgcaagg ggtacgtcaa actttactga tgagtgtcgg atttagtctc
     5041 ttagcaggtg ctatcgtcat tttcttcggt catttctttg ttcaattgtt tgttagccca
     5101 tcagaaacac gtgtatttga attagcacaa acctacttca atatcaatgg gtctctttac
     5161 tggatcttag cagtcctgtt cattttacgc tacacacttc aagggcttgg acaaagcaag
     5221 atccctacga tcgccggtat gatggaattg cttatgcgtt cttttgcagc tatcgttctt
     5281 acaggaatgt ggggataccc aggagcagca gcagcttctc ctttagcttg gatcggttct
     5341 gttgccgtgt tgctttactc ttatatgcgt tcgatgaaac aattgaaaca catgggtgaa
     5401 gaggaacatt tcacattaga acatgcagaa gcaagatagc cttgattaaa agaaatcgtt
     5461 gcggctaaaa atgaaaactt ttattgaaaa tatcttcttc gtttaccgaa aacaggtttt
     5521 ttagataaca aaaaggctgt gacaaaaaat gtcacagcct tttttctttt attctatttt
     5581 tctttccgtg gactctactt tgaaataatc gatatgtgca tgctggtatt ctaataccgt
     5641 gaaacggaat gatgcaatct caatgattgt tcctgctttt atttctttat atcggctaga
     5701 agcaaagccc gcaagtgtag taaaattcgt ttcttcaaac tctggaatct ctacatgaaa
     5761 ataacgctgg aaatcatcta atgttagttt gccggagatg atataagagc catctgactg
     5821 ttccgctatc aatgcttcgt ccaaatctgt ctcatctcgg atttccccga cgatttcttc
     5881 taaaatatct tcaatcgtca ctaagccgct tgtccctccg tattcatctt ttaagatagc
     5941 aatatgcttt cccttttttc gcatgatcgt caataatttt tttataggga tcgtttcagt
     6001 tgttacgatc ggctcttcaa tcaactgttc tactgtcagc tggtcatctt tataagattg
     6061 cttgatcaaa tctggcaagg tgacataacc taaaacattg tcttttgatt ctaagataac
     6121 cggatagcga gtatgtcctt gttcaatcga agcttttatc gcttctttga cggtttctga
     6181 agcttcgaat acttgcatgg acgtacggtc tacttgtata tcttttgcca aggtatcatc
     6241 aaattcaaac acgttttcca aataatgata ttcttctttg gtcaactctc ctttttcata
     6301 agaattcaat gcgactaatg tcagctcttc ttgagacaat gtctcttctc cttcagatac
     6361 gagacgcaca cctaatagct tccctagccc tgcagctgaa tgattcaata cccaaataaa
     6421 aggaaacatt actttataaa aataatgaag tggtttaact acgaacaaga cgaccttctc
     6481 cgtattgacg atactatagc tcttaggaag cagttcgcca ataaccacat cgatatacgt
     6541 aactaaaata aatcccagcg ccacagagat tgtactgcta atagccgaac taaaaggtag
     6601 tttaccgatc actggttcta gtaaagccgc gacagatcct tcaccgaccc acccgatgat
     6661 caaaccggtt agcgtaattc ctaattgact agctgataaa tagttatcta aatggtgtgt
     6721 aacatgaaga gctagcttgg cgttctttac attttccttt tctagttgtt ccaatcttga
     6781 tcgtctgatt ttcactaaag caaattcact tgctacaaaa aatgaagtaa ttgcaatcat
     6841 caaaaataaa ataatgaaat tgataaccat attctcccgc ctttctcttt ttctattgta
     6901 ccctttttct acaaaaagat tgaaaataat gcgtattcat atttaggaat aaaaaaggag
     6961 atgcggcatt tttcagccac atctccttat cacttcaaaa tcattgcgtt acttgttgct
     7021 aaacagcttt ttcatgacct ctgataaacg gtgttcaagt agctgacatt cgacattagt
     7081 tcaggtcaaa gcgctacttt cacaaccgct ctttcaacca tgttttccga gtatttccaa
     7141 tgataagcgg aacagtttca tgttttactt cgctgtactc tagcccaaac catgtttctg
     7201 gcgaggtagc gatttttttg attgccagtt ttgcctgcat gatttgacgt tgccattttc
     7261 ctagagttgt cacattggaa actacttcat caatcaggac aaattggaaa tcgccaactt
     7321 ctcgccctgg tgtcagagag tatctttgtg gctgttttgg caaacggcct tgtttcatca
     7381 aatcatggat gacttggcga acatatacgt tgatttcctg ttggaccctg aaacctaaga
     7441 acagattcac ttggacaata aaatcagtgt ccatcatatc tacttgatat tctttcgtat
     7501 atggctcatc cgtcacttcc acatgaacaa accaatagac tcgtgctctt ttaggccgtt
     7561 tatctaaaat cgaatagatg aactgtcggc caactttatc ttcagccata tcaggaacca
     7621 agaagaccac atttgtctga tagattggta aagaagtatc atcacgtaat tctctaagct
     7681 gcgggatata atctctcaaa gacacatcct cggttgacga ttcgcgaata atgttccctt
     7741 gttcccaaat aaacatcacg actaagatga tcaaaccaat cagaacggct acaaaaccgc
     7801 ctcggaagaa tttgacaaca cttgagataa agaaaacgac ttctatcgca ccgaagaaaa
     7861 aggtaactaa atatgcaagc caacgagaat gcccattttg gataagataa aagtaaagca
     7921 gtgctgttgt catcaacatc gtcacagtga tcgatagacc gtaagcagct tccatatgat
     7981 gagacgttcg gaatgtgaca accactagag aacaggcgat ccataaaatc agattgactg
     8041 ctgggatata catttgcccg atactgttcc caggatacat gatctttaac cgaggcagca
     8101 gtttcaactt gattgcttct gaaactagcg taaacgaacc tgtaatcaat gcttgcgaag
     8161 caatgactgc agcgactgtt gcaaaagcaa cgccgatcac gatccaactt tgaggcatca
     8221 tttggaagaa tggattcagg ttcttgattt gctgtgcttc agggttttga taaacattca
     8281 acaaccacgc agcctgccca aaataattga taaccaaaca aattttgacg taaggccagc
     8341 tcagacgaat atttcgtttg ccgacatgtc caagatcgga ataaagcgcc tctgcacctg
     8401 tcgtagccag gaaaatattt ccaagaatga acaagcctaa tttgttatct gcactaaaaa
     8461 gcagctggat agcataataa ggattcagtg cacggataac tgtccagtct tgactgaagt
     8521 tcattaatcc cattacgcct aaaaaagtaa accaagcgaa catgattggt ccgaatgctt
     8581 ttccgacaat atctgtacca aagcgttgga ccatgaataa aacaaaaata ataactaaag
     8641 tgataatgac gatcacgttc tggttattac cgaaccggtc aaaaaactgc ggaattcctc
     8701 gcaagccttc aatcgcagtc gttaccgtca cagcaggggt caatacccca tctgctagta
     8761 aggcagctcc tccgatcatt gcaggaatga tcaaatattt cccacctttt cggacaagtg
     8821 tgtagagtga gaaaattcca ccttcaccat ggttatctgc attcaatgca atcacgacat
     8881 atttgattgt tgtcagtaga gtcaatgtcc aaaaaatcaa tgagactgat ccgataacaa
     8941 aatcaggatt gattcctgac aatccgccat tatcttcaac aatcgatttc ataacgtaaa
     9001 gcggacttgt cccgatgtct ccgtagacga ctcccatcgc tacaagcagg ccagccattg
     9061 atatcttttt ggtattgtgc gttgttgcca cgtttttccc tcatttcttg ttatatggat
     9121 atcctttagg taagcttgaa tgatagatga tattcttatc catgacaccc gctttcctaa
     9181 attgcttcta attatccaag taacacactg aagtgtcaac ttatttgaat aaaattattc
     9241 tttctttgga cgttctctga tacgtcttgc cacaaatgaa taaagagcaa ggcaaaaaat
     9301 tgcacccaat aatgctggta tcaaataaaa accgccgagt tccggtccca gtggtcctag
     9361 taaaaattcg ccgagccaac tacctaaaaa accgataatg atatttccag atacgcctcc
     9421 cgggacttcc tttcctaaaa ttgcgccagc tatcaaacct aatataccgc cgataaataa
     9481 cgaccataaa aaattcagca tctcctttcc ctcctttatc catatttctc acttttattt
     9541 tacgctttat tcagaaggac tccaaaaata tgcgccaaga aactccagaa aaaaagaagt
     9601 tgtaacaaaa gtgttcagct tatttccgta gaagctgctt ctggaacacc gtgaa
//
LOCUS       DACENJ010000097         9599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.098, whole genome shotgun sequence.
ACCESSION   DACENJ010000097 DACENJ010000000
VERSION     DACENJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.098"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(16..1947)
                     /locus_tag="IW748_001665"
     CDS             complement(16..1947)
                     /locus_tag="IW748_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1104578.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1965..2879)
                     /gene="pfkB"
                     /locus_tag="IW748_001666"
     CDS             complement(1965..2879)
                     /gene="pfkB"
                     /locus_tag="IW748_001666"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1104579.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2876..3628)
                     /locus_tag="IW748_001667"
     CDS             complement(2876..3628)
                     /locus_tag="IW748_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1104580.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3787..3996)
                     /locus_tag="IW748_001668"
     CDS             complement(3787..3996)
                     /locus_tag="IW748_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104581.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4007..5335)
                     /locus_tag="IW748_001669"
     CDS             complement(4007..5335)
                     /locus_tag="IW748_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104582.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(5700..6287)
                     /locus_tag="IW748_001670"
     CDS             complement(5700..6287)
                     /locus_tag="IW748_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1104583.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6370..7620)
                     /gene="clpX"
                     /locus_tag="IW748_001671"
     CDS             complement(6370..7620)
                     /gene="clpX"
                     /locus_tag="IW748_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1104584.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7867..9156)
                     /locus_tag="IW748_001672"
     CDS             complement(7867..9156)
                     /locus_tag="IW748_001672"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1104585.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9310..>9599)
                     /locus_tag="IW748_001673"
     CDS             complement(9310..>9599)
                     /locus_tag="IW748_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104586.1"
                     /translation="LPKPQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYP
                     VYDTYIEDPKRWFQLTVEACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
BASE COUNT         2667 a         2040 c         1633 g         3259 t
ORIGIN      
        1 tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata aaccataaat
       61 cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg ggtgactcag
      121 taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga aagctccgac
      181 taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa tcgggtcagc
      241 tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga tgttcgtcaa
      301 accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag caacgaaaat
      361 tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac ttccgccagt
      421 tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt tgattggacc
      481 gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag cattcgaacc
      541 actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag ggacattgac
      601 cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga ggatcgtttt
      661 aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata cgatgacata
      721 accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg tattagctaa
      781 tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg aagcaataaa
      841 tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt tgaaccagct
      901 agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc caataaattg
      961 gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag gtaacatgtg
     1021 tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat ccgcatttcc
     1081 ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac cactaagggc
     1141 tagattgatc aactcttctg gtttacggat accatcactg acaggacgat tgatcagttc
     1201 ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta caccttctgc
     1261 acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt tcgtttcgac
     1321 tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg ccatgtaagt
     1381 gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt ctggatgagc
     1441 agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt tttgctcttc
     1501 agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta attttcctac
     1561 gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat gtgtgtcgtt
     1621 tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt ctaaggcttc
     1681 ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa ctgcactgtt
     1741 tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg cttcgcgggc
     1801 tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa acattttttg
     1861 gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga tcatcgcttc
     1921 tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc aatttttgtg
     1981 atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc atcagaaaaa
     2041 gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc tttcgtacgg
     2101 ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga atttttcaat
     2161 gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa agcaccgtct
     2221 ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt cccatacgga
     2281 atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc gtgattgttt
     2341 ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc tgttgtatca
     2401 atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata aaatcctttt
     2461 cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc tactgttcct
     2521 aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa gccattgatc
     2581 tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt tacggttgtg
     2641 aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc agtgaaccct
     2701 cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg ggaaacattg
     2761 atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc gcctagtttt
     2821 aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt gtaaatcatt
     2881 gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct aatggacaac
     2941 gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac aactggttga
     3001 atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct aaacgtttaa
     3061 cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca ccgttcattc
     3121 ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta gatcctaaaa
     3181 cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc tccaaatcaa
     3241 ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt ccttgcagaa
     3301 aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct tcgtcattaa
     3361 tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg agtgattttt
     3421 cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca tgaactcttt
     3481 ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat tcagaagcat
     3541 tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc aagtctagga
     3601 ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa ataatagcat
     3661 attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaagtt ataaatcagt
     3721 caaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt ctatcatcaa
     3781 gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag aagcaggttt
     3841 tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga ccaaaaaacc
     3901 gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata ataatgatgc
     3961 cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat attttttgta
     4021 aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga tgctgctcac
     4081 ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg aaggtaacat
     4141 cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct gaaggaacat
     4201 agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct acttctagat
     4261 attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca gtcggatcga
     4321 taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa tacgcctgag
     4381 catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata tcataacttt
     4441 ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca ttctgatact
     4501 ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt ttcaataaac
     4561 cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa taggattgaa
     4621 acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg atacatatga
     4681 cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa atcggtttag
     4741 ggatgatttg atgataatag cgactataat acatttccca acacgaagtg aaacataaac
     4801 cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact tccccgtttt
     4861 gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat agccgttgag
     4921 agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag gcgaacaccc
     4981 catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt tctttgaaaa
     5041 cagagataga caatacattt tttatcaata attgattatc ttccaagcga aaactacatt
     5101 cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct agcattattt
     5161 tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag cggataaatg
     5221 ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt tttttttctt
     5281 cttcatttaa gtctgtgagc tggatagtca tcaataattt atcttcgcca atcattttct
     5341 cacctctttc aaatcttttt ataatttaat tataaccaat gataaagaaa aactaaaact
     5401 ttatgtctta gaaaccaatt tggacgatta catgatagac taagtaaaaa gaaacaaaga
     5461 ggtgtttaca atggggttga aatttgaacg tacagataat ttagataaac ttttcgaatc
     5521 atttgctgtc gatccggaaa aaacagttgc accaaaagaa gagatcgatc gatttctaaa
     5581 accacagcca caaaatgaaa agaaaaaaga caagaaacaa aagtaagtta agctcaaaaa
     5641 aagacttacc agccaaactt ttatgaaaga gtttgactgg taagtctttt ttattctttt
     5701 tatgattcaa ttgcttgttc gattgctgcc cacgcttctt cttttccctg ttttgtaacg
     5761 gaagaaaaga gaataaacgt gtcattgcga tcaaaatcca acgctttttt gatttcgctc
     5821 tcatgcttgt tccatttacc acgagggatc ttatctgcct tagtggcaac aacaattaca
     5881 gggatatcat aatacttcaa aaattgatac atctggatat cttcttgaga aggcttatga
     5941 cgcaaatcta ctaaagaaac gacagcacgt agctggtctc tagtcgttaa atatgtctcg
     6001 atcatctttc cccattttgc tcgctcagtt ttggatactt ttgcataccc atatcccgga
     6061 acatcgacaa aatgtaatgc gttctcgatc aaataaaaat tcaatgtctg ggtctttcct
     6121 ggcttaccgg aagtacgggc taaattcttt cgatcaatca atgtgttgat aaaagaagat
     6181 ttccctacat ttgatcgtcc tgccagagct atttctggca attgtgtatc tggatattgt
     6241 tttggtgcca ctgcacttat gataatttca gcttgatgga ctttcatggt ctctctcctt
     6301 aacaaggtga tgaataaagc aagcggctgt cgcaaaagcc aaaatgactt ttacggcagc
     6361 ctcttttcat caacccgctt ttttctctga atctttatag attactgttg gctcaccaga
     6421 caattcagct gcttcttttg tgataatcac tttttcgatg ttttcatttg aagggatatc
     6481 aaacatcaca tccatcatga tttcttcaat gatggaacga agtcctcgag caccggtatt
     6541 gcgttcgatt gcttttttcg caatggctct caacgcttca ggttcgaatt caagttcggt
     6601 attatctaga gaaagaagtt tttgatattg tttgactagc gcatttttcg gctcagtcaa
     6661 gatccgtacc agatcatctg ttgtcaattt ctctaaagct gccataacag gtaatcgtcc
     6721 gataaactca gggatcaatc cgaattttag taaatcttca ggaatgatat gctgcatgac
     6781 actttcgtct tcagaaagtt tttggttttg tgtaccaaat ccaatcgtct tttcgcccat
     6841 acgattcttc acaatcgttt cgatgccatc aaatgcacca cctacgatga acaatatgtt
     6901 cgtcgtatca atctgaataa attcttgatg tggatgttta cgtccgccct gaggtggcac
     6961 acttgcaaca gttccttcta gaattttcag caaggcttgt tggacacctt cgccggaaac
     7021 gtcacgagta atagatacat tttcgctttt acgcgcaatt ttatcgatct catcaatata
     7081 gatgatccct ttttctgcaa gttccacatt gtaatcagca gattgaagca atttaagcaa
     7141 aatgttttct acatcttccc ccacataacc agcttctgtc aaacttgtcg catctgcgat
     7201 tgcaaaaggt acattcaatg tttttgctaa tgtttgtgct aaaaatgttt taccagaccc
     7261 tgtaggtccg atcaagcaga tattgctttt ttgcagttca acatcatctg tgttttcttg
     7321 tgcttgctga ttgacacgtt tatagtgatt gtatacggca acagctaacg agcgttttgc
     7381 gcgatcttgt ccaatcacgt agttattcaa tacttcaaga atttctagtg gtttagggac
     7441 ttcagtgaat tcgcgtacag cttcttcata aaattcttca tcaatgattt ctttacatag
     7501 gtcgatacat tcattacaga tgtagacccc tggaccagct acgatctttt tcacttcttc
     7561 ttgggttttc ccgcaaaaag aacagcgtac ggtctcattg ctacttggat tgtcatacat
     7621 ggtcttcacc ccttaacttg caaaaagtat gcaagaaact atcaaagact ttacagtctt
     7681 ttactatcat aacatagttt agtaaaaaga aaaagtgttt aacaaaggat caactgacct
     7741 atacctttgc attttttact cttaacaaaa aataaggaac tgtgacaaaa tcgtgtcaca
     7801 gtcccttatt tcgaataaac aattttctag aagtagcttc ttcagaaatg atttattact
     7861 tgatttctat tctttcgctt ctgtcgctgt acctgtgatc aattcgattg cttttttcat
     7921 tgtcacatca tgttccagca tatcttctgt caacacgcgt ttgatttgtt cgattggcat
     7981 gttgtatgtt tctgataatt cttggatttc tgcattgatt tcttcttctg ttgcttgaag
     8041 attttcagca gctgcaattg cttcgatcac aagatttgtt cttgtgcgca tttctgcttc
     8101 accttcgaat tgtttatgca agtcttcttc tgtagaacca gttaattgat agtacatttc
     8161 aggagagata ccttgacgtt gcatgttgtt caagaattcg tccattgaac gatgtacttc
     8221 atcatgaacc atcacgtgtg gcaattcaac gatttctgcg ttttcaacag ccatacggat
     8281 cgctgcttca tcttttgcat cgtccgctgc tttttctttt gtttctacta attctttgcg
     8341 atatttttct ttcaattcgt ctaatgttga tacttcgtca tcaacatctt ttgcgaattc
     8401 gtcatctaat tcaggcactt cttttgcttt tacttcatgg attgtcactt tgaagactgc
     8461 ttcttttcca gcaagatctt ctgcttgata atcttctggg aaagttacat ttacatctag
     8521 actttcacct gatttatgac caactaattg atcttcaaaa cctggaatga atgagcctga
     8581 acctaattca agtgagtaat tttcgccttt tccgccatca aacgcttcgc catctacaaa
     8641 gccttcgaag tcgattacta ctgtatcgcc attttcagct gcagcatctt ctttgataac
     8701 taattcagct tgtgcttctt gttcacgttt gatacgtgca tccacgtcag catctgttac
     8761 ttcacggtct tgtttttcaa caactagatc tttatattgt cctaatttta cttctggttt
     8821 cactgttact tcagcagtga ttacccagtc ttgacctttt tccatgcttt caacatcgat
     8881 ttttggttga gaaacaggat cgatgccagc ttctttgaca gcagcttcat aagcttctgg
     8941 taatacagca ttcaatgcat cttcatataa agcttcttca ccgtacatac ggttgaatac
     9001 ttgacgagaa actttccctt tacggaatcc aggtacgttc aagctttttt tcactttatt
     9061 aaacgcagtt gttaatcctt tttggatttc gtcttgcgca atcgtaaatt ttaaaacacc
     9121 atcattcgtg cctttttttt caaatgttgc agtcatttaa ttccctccga gtttagaaat
     9181 tcattttata catcaaaaac gtttcatgca tacctttaaa tagtacacta accctttcaa
     9241 attgtaaaca tattgaatgg atagaatcct tgtttttcat aacattttta tctttttcac
     9301 tgataaaatc taatggaaga agatcatttg ctgcaaaatt ttttctcttt tttttaaaaa
     9361 atcttgtttt tcatcttctg taccttcata catcgttttt cctgtacacg cctctacagt
     9421 cagttgaaac cagcgcttcg ggtcttcgat gtatgtatca tagacaggat aaacaattgc
     9481 agattgcaac gtgaattctt cttttaacac ggaactaagc agcgcatctt cattcccgta
     9541 acgatctgaa aatacacata attgctggta acttgcctgc tgctgcggct ttggcagat
//
LOCUS       DACENJ010000098         9561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.099, whole genome shotgun sequence.
ACCESSION   DACENJ010000098 DACENJ010000000
VERSION     DACENJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.099"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1115
                     /locus_tag="IW748_001674"
     CDS             <1..1115
                     /locus_tag="IW748_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1104587.1"
                     /translation="FSLSSMITSFHISGTQAGFISTITNLGMLIGGIFFGIIADKYGR
                     VKVFSQTVLLFSIASLLMYFASNIYLVYLFRFIAGVGAGGEYGACMSLISESFSKKQI
                     GRASSVAGIGAQVGAALAAILAAVIIPTFGWKMLYVVGVLPVLMVLFIRRGLTEPKEF
                     KSVQSAEKKTKLSRLFESKHLAWQTCGLSIMVTVQIAGYFGLMNWLPSIMQNRLGLSV
                     SGSSLWMVSTILGMSLGMMTFGIIMDKLGPKLAFSLFLICSSLSVYLLVLAHSQWSLV
                     AAAVIVGYFINGMYGGYGAVISSLYPTEIRATANNFIMNLGRAVGGFSSIVIGFLMDH
                     YSLTAVILFLSGIYLVSLLVLTTLTGVTALRESLQH"
     gene            complement(1281..1703)
                     /locus_tag="IW748_001675"
     CDS             complement(1281..1703)
                     /locus_tag="IW748_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1104588.1"
                     /translation="MQTFLTKASQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            2293..2748
                     /locus_tag="IW748_001676"
     CDS             2293..2748
                     /locus_tag="IW748_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104589.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(2910..4082)
                     /locus_tag="IW748_001677"
     CDS             complement(2910..4082)
                     /locus_tag="IW748_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1104590.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(4288..5717)
                     /locus_tag="IW748_001678"
                     /pseudo
     CDS             complement(4288..5717)
                     /locus_tag="IW748_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(5714..7216)
                     /locus_tag="IW748_001679"
                     /pseudo
     CDS             complement(5714..7216)
                     /locus_tag="IW748_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(7213..9540)
                     /locus_tag="IW748_001680"
     CDS             complement(7213..9540)
                     /locus_tag="IW748_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1104591.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         2610 a         1854 c         1823 g         3274 t
ORIGIN      
        1 ctttttcatt atcttcaatg attacttctt ttcatattag tggtacgcaa gcgggtttca
       61 tttcaacgat cactaatttg gggatgctca ttggaggtat tttttttggc atcatagctg
      121 ataaatatgg acgtgtgaaa gtattttctc aaacggtcct tctgttttcg attgcttctt
      181 tattgatgta ttttgcttca aacatctact tggtttatct ctttcgcttt atcgcaggtg
      241 taggagcggg aggagaatac ggggcgtgta tgtccttgat ttccgaaagt ttttcaaaaa
      301 aacaaattgg acgagcttct tcggttgctg gtattggtgc gcaggtcggt gcggctttgg
      361 ctgcgatttt agctgctgtg atcattccaa cgtttggttg gaagatgttg tatgtcgtag
      421 gtgtactgcc ggtgttgatg gtcttgttca ttcgtcgcgg cttgactgaa ccaaaggaat
      481 tcaaatctgt acaatctgcc gaaaagaaaa ccaaacttag cagactgttt gaatcaaaac
      541 atcttgcttg gcaaacatgt ggccttagta tcatggtaac tgtccaaatt gccggctatt
      601 ttgggttaat gaattggctg ccttcaatta tgcaaaatcg attagggctt tcggtttctg
      661 ggtcttcatt gtggatggtc agtactattt taggcatgtc tttggggatg atgacttttg
      721 gcataatcat ggataaattg gggcctaaac tagccttttc tttgttttta atttgttcat
      781 ccttatctgt ctatctactt gtgttagccc atagccagtg gagtttggta gcagccgctg
      841 tgatagtcgg ctactttatc aatggcatgt atggtggata tggcgcagtg attagtagtt
      901 tgtatccaac agaaatccgc gcgacggcga ataatttcat tatgaacctt ggacgagctg
      961 ttggtggttt ttcttctatc gtcatcggtt tcttgatgga tcattatagt ttaactgccg
     1021 taatcttgtt tttgagcggt atttacctag tcagtctgct ggtcttgaca actcttactg
     1081 gtgttacagc attaagggaa agcttgcagc attgatagtt gagtctgtaa atgacaagaa
     1141 agaggtgtgg caaaagcttg catctgcggt aataagacca gccaaatcaa aaacatgaga
     1201 cactgtttcc gtttggctgg tcgtattact tgatgcaggg cagctttttc aactgctaat
     1261 ttccttctat ctggcggcta ctattctttg acgtccgtca tttcaaagta ctttactaat
     1321 aacttagcat aacgaaaaac tgtggataca ctcgtatatt gagcgtctgc ataatcttcc
     1381 aaatttttct caggatgttg atacatgaat tcaatcaaat gataattata tgattttctg
     1441 tagaaatgaa gataaataat gtagattggg aagttctcct gcttttccat agaaaagcgc
     1501 cgattattct cattcttcaa aatgacctta tcaggatatt tttggaacaa ttcttctaaa
     1561 tctgttagat aattcaagat agacttactt gataccttca attcgttttg tagttcttct
     1621 attgaacacc aacgttctgt ttcaagcagt aaatgaatca ttttaatttt cttttgagaa
     1681 gcttttgtta aaaaagtctg catattttct cctcctgtat ctactttact atactacata
     1741 caagaaaaaa aacggataca aatatcatat tgtcacaccg tcaatagtgc ataaacctgt
     1801 tattatttct ctttttttcc taagtattct attgatatga atcatttaat cgtttattat
     1861 ataaatgtca ataatctaat aataaaataa cgtacatatt ttattattgc atacttaata
     1921 acagaactat agcaaaaaat cccgtgtatc aaaacgggat tagattaagt ttcctcaatc
     1981 actattcata acacaattat tataacaagt aagtaaacaa ttgaaaagga catttattga
     2041 aaacggttgt tttttactta aggaggaaat ttagtgaaaa aattagttgc attgtttttg
     2101 gtcgcaggaa gtttgttgag tgggtgtggt gctactacgt ctactactca aagcacaagt
     2161 tcagctcagg cttccagcag cagtaccacg atctccgaaa gtacggcaga ttcatcgaaa
     2221 gaacaagaac aaattgaaaa gaaaaaagaa gaagagaaac aaaaagcgga aaaagctgca
     2281 ctagaaaaaa aagtgacaga agcggaatct gctttgtcac aagctgaagc aaatcctaca
     2341 aaagaaaact atgcaactgc ttcagcagct atccaagcgc tacctgaaaa caaacaagag
     2401 ttgacaagcc gtcttgctgc aattgattcc acaatcaaag caaaagaagc agaagaagaa
     2461 cggaaaaagc aagaagaaat tgcacgacaa caagcagaac aagcgcaaca agaagcaatc
     2521 gcacagcaac aagcagaaca ggcgcgacag caagcagcag ctgaacaggc ctctcagcag
     2581 gcacaagccg cagctcctga gcaaaataat gaacaaactg tctatgtcac accgaccggt
     2641 tcaaaatacc atacacataa atgtggaaat ggtacgtata cccctgccac tttaagcgaa
     2701 gctcaagctc gtggtttaac cccttgctcc aaatgttatg gaaactaatt caaaagttat
     2761 actataataa taaaattctt ctgaaacata aaaaaataga agctggcgtt ccagatagta
     2821 aaccaaaaat agcagtttac acgtccattt cgtcagcttc tctctttttt catttagtct
     2881 gttccctctg cttttttgga catcttcatt cattctttct caatcaaata gtacacctct
     2941 ttttcttttc gcagtttata tcttcctttt ttcaatttca ttgaaaactg tttcgaatgt
     3001 ttatgtttta catggatttt aagcaggagc tcctcatgtt gcaagcgata gttcctgcct
     3061 tttaaatcag aaaatctcac tagtccgtat tcatcagaag ctttcacaaa tggaactttt
     3121 tctctagtaa tatagtgttt gccgttgagc gtttttacag aaaagaccat tccttcgatt
     3181 ggtcgtccgt ttgtgttctt tacataaaaa gataaaggat agtttcgctt agcgatggcc
     3241 aaagaatcta aataagcgat gagtagccaa actaagccga ttgatccaag tagtataccg
     3301 ataaaccaaa taaaaaattt ccattttttc cattgatcaa ttccttggat cgcttttttt
     3361 gcctcttttg cttgataagg aatacgatgc cccgtcacaa gcagtcgatg tgtattgacc
     3421 atataaggag tacaggtaag caaagtcacg agatctctgc ctttttggat acccaaagaa
     3481 tctacttcat caggtaagac cacttggatt ttttctactt gatacgccaa tgtctttcca
     3541 ttgatctgga tgaaaaactg gtctcctttt ttcaacttag gcaaatctgt aaataatttt
     3601 gcttctggga gtccacgatg cccagaaaga acggcatggg tgtttttccc gccagtcgga
     3661 taagaacttt cttccaataa tgacgttcct ttttgcaaaa atatttccgt tgtctgatcg
     3721 aatataggta agctgacatt gatttttggg atttcgatga ctccaagcat atgttccata
     3781 aaaaaggcat tactaggtaa atcttttaat gcttttgcat caactgcttg gttgaaactg
     3841 cctatccctg gtgatacgtt ttgctctgcc aactgttggt ttttctttgt cattttttcc
     3901 tgctgttttt ttatttcctc tgaattctta ttcgatgctt gcttttgata atggatgatc
     3961 atttgctggg caagaaaatc attcagtgaa tcttctacaa aaggataaga aaagatcccg
     4021 atcccaacga ctaaaatgat tgccatgaag atgtccagta ttcttattct gcttttattc
     4081 atcatgtcca cctattttct aatatgtata tgaagaggtt gtgaaatcag cgccttgacc
     4141 tgagtattcg ctcaacaaac ggcaaaatag ttcctcctat tttgccgttt gttgagcgaa
     4201 taccaagaga ccgaccactt gtcacaacct ttctccccat ttgaatcact atcgtttatt
     4261 tatcgtatga tttttgtttc caatagttta agcttctgtt tcttgatggc gttttttgaa
     4321 gtagatcact gctcctccaa ctaaagcagt acctaccaag atgaatacga tgatccccat
     4381 accaccagtt gaaggcaaga ttcctttagg ttggttatcg atcgttgttt catcagaagt
     4441 tgttggatca tcatctactg taaattcagt gtacgcagct tcattcttag gtaatgcgta
     4501 gccttctggt gcttttgttt cttgaaggca gtatgcccct tgtttcagcc cttcgattga
     4561 aaatttccct tctgaatcag aaatcaattt cacttcttcc cagtcagaac ctgtgacttc
     4621 atctgaccaa gcatatgggt attctcctga ggcaagattt gctatttttt tcattaattt
     4681 aacagttgga tcttttgctc ttagaataga aaattctgca tcggctaatt tttcttgcgt
     4741 gttcacatct tgtttgatga agtggaaatc accgtagttt tggacatttt ctacagtaat
     4801 cgtgttatcc gtagttgtat tttcccgatt gactacaccg tttggattcc caagattggc
     4861 ttcatctgcg ccatggatga taccggttgt ttgttgcaat tttccgtttt ttactataaa
     4921 agtagtttct ttttgtggat tggttaattg ataaccatct ggtgcttttg tttcaactaa
     4981 agtataattg ccgtctatta atccattgac actgatggat ccttcagtat cagttgtgaa
     5041 tgtcaacgct gactcagcag gatcatagag agcgacacca gtgttttcat caaaaccggt
     5101 cacgaattga ttctctgaat ttttgatctg gaattgcgcc cctgctaact tatctacccc
     5161 tgaaaagttc ccagctttct ggattttgat attgctttct ttgatgacat ttttagcata
     5221 taggtgaatc gtgtctaaag gtttgtcagg gtctgatgaa ttcgggttat aaaagggtaa
     5281 gcttaacacg atatttggcg acgtttcgac accgggcttt ggtgtttcta caaagacgta
     5341 tacagcatct ttcccatcag atttacctgg aagagtcagg atagctaatc cttcaccaga
     5401 agtcgttaca cttgcgaagg gtgtagcgtc tgctggaact gctgtgtctt tacttggata
     5461 gtacgtagca gtgatgtcat atacgttgaa ttcaacgccg ttcaacggtt cgctattcgg
     5521 aaatgtcaat tccgttccgt tgtttatttg attttctggt gctgaagtga atttcaattt
     5581 atgcaaatta accgtaacat tttctggtgt aggtgatacg gcctagaaac ctctgcagtt
     5641 cctaggccga gaacgaaagg aagcagcatg atagcagttc ctaaaaacgt ccatattttt
     5701 gttttgatgt ttttcatttt atttccctcc tattgtttcc tttttgttgt tttttataaa
     5761 accaagctgt ccctgttgaa aggactagca acaaccctaa aattgagaaa cctccaagca
     5821 tctcttcgct tgtttttgga aagcttcctc catttttttc atttgatgga ccatttgtct
     5881 gcatctgaac tggcgttttt ccattattcg tcggatttgc ttttggttcg ttgatgattt
     5941 ccaatggtat aggatgaagc ggatcataac tgcctttttc aacttcaaag agaatggttg
     6001 attgactcaa tatatatcct ttaggcggtt taatctccac tagttcgtaa cttccaaaag
     6061 acaatccatg gacagaaaat gttccatctt tactggaagt aaaaatcgtt gctcttgaat
     6121 catctttggc tacccaatga tttatcccgt tttcttgcgc taagtattgg tcattttttt
     6181 tgactagaaa ttgagcatct gctagttttt gtttcgtttg ccgatccact ttgacaaatt
     6241 gtttgcctcc tgtttggatg gcaagatgtg tttctactgt ttgattgtca gtcgtcaaac
     6301 gtacatcgtt atcaaatgtc gttttttcac tgttttcttt caatgtcatt tggtaacgga
     6361 tcgttaattt tttcccagca aacgaactaa gatcttttcc agaatctgat aagacaaaac
     6421 catgttttgt tttttgtgtg tgaaatgtat gaatcttgtc tccaccaatc aaaaaatcga
     6481 tcgtttctgg ttctagccat aactgaggat cagctgtatc tttgatcgta taggttccta
     6541 tctcgttgat gttctcagga attgttacgg tgatgagata aggaacttta tccccatctg
     6601 caaaatctgt ctgcttattc tctactgttt ttgttaatgt gagtgcagca ggtatttttt
     6661 gttcgctctt tggatacaag tggatggtcg tcaatttatg attagatgaa tctgtcacag
     6721 gaagcacaac gacaaatggt gctgattttt cttttactta atctgaagtc tttatttccg
     6781 caaatcggta aacagcgtca cgaccttgct catctttgtc cgatgcacta aaagaagcaa
     6841 tgccctcttc ctcgtctact gtttttgtca cattttctgc cagtattttt tcactatcac
     6901 ttttttgtgc tagttttctt tgtgcgtctt ccacactact tccttctgac cgcagcttgt
     6961 aaaattcatc cgagacatca tatatagcga aagtaacgcc gttcaaccct tttactcctt
     7021 gaaagttatc cgggatattt attccgtcgt tttgactgat acttggcatc ttatcgcttt
     7081 cgaacattcg attatgcaaa gtaaatgtaa taggtgattc tctagctgac acagtcggag
     7141 aacctataaa aaagaccagt aggataaaaa agagaacagc aaatatatcc acacgtacat
     7201 tttttgtttt tttcatcgct gttcacctgc ttttttctta cgatggataa aataaaagaa
     7261 gccagaaaaa ataaagatca gtcctaaaaa gacaaagggc agtagaccta tcccacctgt
     7321 cgaaggaagc aagagagtcg gattgatcgt caacttagcg gtatctgttg cgataatcgt
     7381 ataattattg tttacacttc cttcgcctaa actaatcgtt actatttgtg atccaatatc
     7441 tgtccggctg ttaactgtta gggtataatc caatgattcg cctaagacaa caccggtaac
     7501 gtggagattt ccaaccgaaa tcgagtgact ttgggcatca taattaaagg cagcgtcatc
     7561 gacagtaatc actgcgttag ctttatagat ggtataagtt cctgatgtgt tggctatatc
     7621 agtaaatgac aacttgggat ttgctgcttt gactttgtct attccgctat tcgttagttt
     7681 aattgtatag ctgccagcat taaccatatt ttttgctgat tgtgcatctg ctgtgtaatc
     7741 catactgcta agtgccactt gtactggagt aagcgaacta gtgacaccct ctgctagctt
     7801 gacatggatt gacgggacac ctgcttttcc atcatatgtc ttactgccac tgagctctac
     7861 cgcaccgctg atttgcttaa tcgtatagac atcagatgtc tgcacgatag taaataaata
     7921 gtttgttctg gcagctagtt tagctagtcc gctttttgtc aattgccaag acgtataggt
     7981 tccaatttca gtaggcgcag ttacttcaac tcccttttca ttcatcgctt tgaaatcagt
     8041 tggattccat gtgatttgat caatactgag atcattgttt aaaagggatg ttgattctgt
     8101 tttatcatta aaggcatatt taacgatcgg tacaacagaa agaccatcat agtacttact
     8161 gccatttgca ttgattgata ctgttgcatt tgtcttataa tataggatga cttcagaaat
     8221 ctgttttcca tttgcgtcct ttacgattac atttccattt gtatctaaac tagctaaaac
     8281 actcattgct gattggttct ttgtttctgt cgctgaaaca gctgtaaagg tatatccgtt
     8341 gatagtaggc gtattagtcg tcaggtcttg tgcagcactt ccgtaagtaa gattcattat
     8401 tttagaagga gcaatcatct gtccaaaata gtcttgatac ttaatagtga ttggtgtttg
     8461 tttgcctgta taggtcaccg tatcgacgtt actttctagg gctgtgaaag aaccgtttgt
     8521 ttgtgcaaaa gaatagcccg ttactgctgt tggagtgata accactttgt cacccacatg
     8581 gattccagat aatgtagtcc cgtacgttcc gatttttgct gctccaccat tgactgtata
     8641 agaaaaagtt cctgtccgat tcgcattgac ctgaaccgtt gctgcttgat aggttggaaa
     8701 atttgaaaca tttcccttac catcaacgat ctgcccattt ttgatcgagt accccgtgaa
     8761 tggaatatca tttgctaact gtataccgat tgaaaaagta ctcatacctc ctccggcagg
     8821 aagccctccc catgttgagg gtgtaaaaga ataactttta tccgcattgg gtgtaactga
     8881 atcgatcgta tttccagcgg tagccacccc catatagata ctttttggga atgtgatagt
     8941 agtaccattt actgtggagc caataaacag attatggata tctggacggt tatcaataaa
     9001 agcgattgct ttggttacgc cttgtaacgc agttttatcc gcactcatcg atccgttaac
     9061 cgcaacaatg actccatctt tggcatatat cactgtatat ccatctggta tggtatgtcc
     9121 gttgaaattc gcagtggccg caacagtact aaatgtctgg cttctacctg tttctgaata
     9181 agctttctcc cctgctgctt ttgctgctgt gatttctttt tctgtcggat caatcagttg
     9241 agtcgaggat gtggttgagg ttgtctcgtt gttgctagac tgattggtgc tttctatgga
     9301 ctgtatttca gaatagggtg gactatttgt ctcctctgtc gtttggttag caaaatttga
     9361 cgtttctgta taaacgttta cataaatact tgataagctc cctaagaaag taaaaatcaa
     9421 gatgatcgaa atcataatgg gatgtaagta ttttctttta gatacagctt tgagtctaga
     9481 aacaaaatgt ttttgacata tacgttggat tttattttga gttttcttaa tcattttcat
     9541 ccctcctctc aataaaaaat c
//
LOCUS       DACENJ010000099         9448 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.100, whole genome shotgun sequence.
ACCESSION   DACENJ010000099 DACENJ010000000
VERSION     DACENJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.100"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..1257
                     /locus_tag="IW748_001681"
                     /pseudo
     CDS             1..1257
                     /locus_tag="IW748_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1364..2395)
                     /locus_tag="IW748_001682"
     CDS             complement(1364..2395)
                     /locus_tag="IW748_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104592.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(2402..3232)
                     /locus_tag="IW748_001683"
     CDS             complement(2402..3232)
                     /locus_tag="IW748_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1104593.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(3229..4035)
                     /locus_tag="IW748_001684"
     CDS             complement(3229..4035)
                     /locus_tag="IW748_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1104594.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(4041..4865)
                     /locus_tag="IW748_001685"
     CDS             complement(4041..4865)
                     /locus_tag="IW748_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1104595.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(4855..5955)
                     /locus_tag="IW748_001686"
     CDS             complement(4855..5955)
                     /locus_tag="IW748_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1104596.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            6133..6918
                     /locus_tag="IW748_001687"
     CDS             6133..6918
                     /locus_tag="IW748_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1104597.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6951..7334
                     /locus_tag="IW748_001688"
     CDS             6951..7334
                     /locus_tag="IW748_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1104598.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            7410..7541
                     /locus_tag="IW748_001689"
     CDS             7410..7541
                     /locus_tag="IW748_001689"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1104599.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            7510..7746
                     /locus_tag="IW748_001690"
     CDS             7510..7746
                     /locus_tag="IW748_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1104600.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(7824..8795)
                     /locus_tag="IW748_001691"
     CDS             complement(7824..8795)
                     /locus_tag="IW748_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1104601.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         2798 a         2168 c         1822 g         2660 t
ORIGIN      
        1 tatcaaacta ttactaggaa tcaaagatgg aaacattcaa atcactcatg tcgagaagaa
       61 aaaaatacat tcaatcaaca gtatcgttgt ttctgccacg ctcattaaaa cgattcagcc
      121 ctgtcctcag tgtaaacagg tgaatcaaca tgggctgatc gttaagaacg gaaagaaaaa
      181 atcgctggtc cttctcaata agtgtgcgaa tcaacggact tatcttgctt tggcaaagca
      241 acggtacctc tgtcgtggct gtcacaccta ttttacggcg aatacctaca ttgttgaaaa
      301 aaactgtttt atcgccaaac aagtacatta taaaatattg gaggaattga ctgaaaaaca
      361 agcaatgacg acgatttcta aacattgcgg cgtttcttgg tcaaccgttt cgcgaacact
      421 cgcttctctg ctccccatga caaacgtgaa aataaattgg ttgcctcgct gttcattggt
      481 agatgaattt cgttcattga aaaatcaagt cggtccctat tctttttctt gtatggatgg
      541 cgatactggg aaattactgg atattcttcc ttcacgtaag aagaaagatc tcatttctta
      601 ttttatgcaa tttgaacgac gtgcacgttt aaacgtaaaa atactcgtaa cagatatgaa
      661 cgcctcttat gctttgctga taaaagagtg ctttcctcat gccaaactcg ttgtggatcg
      721 ctttcacatc gtcaagcacc tcattcgcag ttttaaggat atttgactat gagtcatgaa
      781 aagcttcgac cgaaacgatc caatacaagc caaacactat cgacaagtga aggctttatc
      841 tcgacttctg atcaaacgac aggacacgct tgtttatgat aaatggacaa aatggcgtaa
      901 ttttggttgg gcctatttaa ccgaaagtga agtcgtagaa cgtctacttt ctacttctga
      961 tgaactacga atagcttatg cttactatca agaaattctt caagcttttt atgacaaaga
     1021 agcggacatc ttctttcagc ttgtaaaaac aatgcccaag tcggttccaa gagaactgca
     1081 tcatataaaa aaagcgttta taaactatga atccggcatt cgtttagcct tagagttgcc
     1141 ttactcaaat ggcaaaatag aaaatctgca tacccacatc aaagcattga aacgaatagc
     1201 ttacgggtta ggatgagaac cagaattttc ttaatcaatc atctgatcac ttattaaaca
     1261 taaaaaaaca tctaaagcag aaaatcaatt ctaccttaga tgttcttata tcactctatc
     1321 agtcctattt gacaaagagc cagaaatgtt tgccgactat acactagctt tgttgtgcaa
     1381 aatgctgagt atcaatttcc acagggattg ctagaatatc tttcagctga atgtataagg
     1441 catcgctaat tttctcttcc cgatcgttca accgatccac taaaatttct ttgccctcca
     1501 gcgtttgaaa aacataccgt tgaatgttgc ctaaaataaa tgattccttc catagaaccg
     1561 gaatcttaac cccctgagga accggttcaa attgaatcag ctccggtctt aaataataaa
     1621 tgcagcgatt gcccttagcg gaaaactcgt gggtatactg attcagctca taccattcga
     1681 aacgattata gttgccgata aattccgcaa caaatcggct ctgaggattc tgatacaatt
     1741 ccttcgaggt ggaaacctga gccacccgcc cagcttccat cacaaaaatc cgatcactga
     1801 ttcgcatcgc ctcttcttgg tcatgagtca caaaaatcat cgtcatccct agcgattgct
     1861 ggatagcgcg caaatcccgc tgcagctgtt ttctaattcg ggcatccaaa gcacttaatg
     1921 gctcatccag cagcaggact ttcggttcag tgaccaatga gcgagccagt gccacccgtt
     1981 gtcgttgacc gccagaaaga aactggacat ttttatctgc ctgctccgtc atatccacca
     2041 atgatagcat ctcaaaaacc ttttcctgaa tcacctcagg actcagcttc tttaccttca
     2101 aaccaaaggc tacattttca aaaacagtca tagttggaaa caatgcatac tgttgaaaaa
     2161 caaagccgat attgcgatcc ttcgtaggct tatcggcaat attctcattg tccagcacaa
     2221 tctcaccgct ctttggttgt tccaatcccg tgatacagcg cagcaacgta gactttccgc
     2281 acccgctggg acctagtaaa gtaaccagct cacccttttc aatctgaatt tcatcaatag
     2341 ctaaaatctg tttttcatca aaaaacagat tgagatcatg tatttctaaa aacattttcc
     2401 gtcacaactc ctttaagggc ttctttttcg actgcttctc ttccctcgtc tgcttaaggg
     2461 acagctgttg cttttgtcga taagtcagca taaaggtagt taaagcaatt cccaccataa
     2521 acagcaaata caaaaccatc acagcgctag ccaaatggcc gttttcgttc atccgccgca
     2581 gcatatagat gcgtaccgtc tgaaatcgac cgccaatcaa caggttcgtc agcatgtact
     2641 ccccaaaaac cgaagaaaaa atcatcagag aaactagggt cacaccgata cggatgtttg
     2701 gaaacagcac ctggataata atcgtagaca tcggggcgcc caaggtttct gctgcatcca
     2761 ctaattcctt cagattaatt aagcgcatct ggttgttaag actaagatag acaatcggca
     2821 acgccgtaat aaacaatgca cctaccaaca ctacaaacat tgggatacca gtttcagcat
     2881 acgttcgaag caaagccgtt accaaaatca ctcctggtaa agcatacggc agcaggagca
     2941 gcttttccag ccaacgattc aaacgaggga aatacaaatg aatccaaata atggtgggaa
     3001 tcatcaacaa cagaatcgtc accaagacca cacctgctaa taaaagtgag cgacccaccg
     3061 cagcaataaa ttccctgtcc gtcagcagct gttggaacca ttgcaaggta aaatcactag
     3121 gtaaaagcga ttttgaccat ttagtggagc tggcatacaa aatcgttcca ataatcggca
     3181 gcaagtaata caggacaaag cagaacaaaa tcggttttat taactttttc acgtctgcac
     3241 caccttgcct ttcttttgaa taatcaacat tgccgttacc agcaggacac ctaaagcgac
     3301 tgccaacgca ctgcctaaat taggcttggc gaacacatct cccgacacta aagccgcaat
     3361 tcgaatcgtt aaaagattta cattactgct ggtcagggca tacgctgttt cataggtccc
     3421 catgccatta gcaaataaga tgacaaaggt gctggcaatc gatggtcgca aaaaaggcaa
     3481 agccaccttg aaccaagcat acgctgatga agcgcccagc aattgtgcgg actccagcca
     3541 ttcctttttc acttccttca tgctgggata caaaaacaag acccctaacg gaatttggaa
     3601 ataggtatac gtgatggtca atccccacca agaatacaaa ttgaagtccg ctagaaatgg
     3661 caaaatcatt ttcagcacac ctacattccc caacaaaata ataaaagaga atgccagcgg
     3721 aatcccagca aaattagctg ccaaattgga aacaaaggtg attttctcct gcaatttagt
     3781 cgatagattc attaagcata aacataaaat aaacgtcccg attaacccaa tcacactgga
     3841 gctgacagca atcagtacgc tgttaacaaa ggcctgtgtg taatatttgt tgctgaaaat
     3901 atcactgtaa ttggccaatg tccactggtt agttaaatca ctgtgcaagc tgcacagaac
     3961 cataaaaagc agcggcagca gtaaaaatgc acagaagata aaggaggcag gaaccagaac
     4021 caagcgtttt ttcatctctt tcatcccctc atcaattctt gaatgtcctg cattttctct
     4081 gacggtgcaa ttcctaataa ccagcaaaac aatggagccg ccagcagctg tggtacagtt
     4141 tgttccttca gcttttggcg tttatttgaa atcaaataaa aaggaacttc acgatgtgca
     4201 gccaagctgc cgccatgcag tccgtgcgca tccatcccgt gatccgcagt caccacgact
     4261 tgataacctt tttgcagcca ttcaggaagg taaatagcca ataatctgtc cgcctgatta
     4321 accgctgctg catattcatg agaatcggcg gtaaatttgt gaccgacatc atcaatattc
     4381 atcgaatgta cgaccaaaaa gtctggctga tactgattca ctaaataatg ggcatccgca
     4441 aataaatgcg aatcagggta agtgtcctca ctataaaaga tgccatgctg aatcagtcgc
     4501 tcctcctgat gctggatgcg atcaaccaaa cgattatagg gggcttgatt gtacaactcg
     4561 ctgtaccagt ggtaagcggc ggctcctgtt tttccgccag cttccttaac taattcaaac
     4621 agcgaccact ctttggatcg ctgagcatgg taattactat agatgccatt ttcataggct
     4681 ggaacgccgg tcatcagtac ttcgtataac ggacgtgaat tgctgggcag ctcacttttt
     4741 accttcaata agcttgcttg ctggtgctca actaaatgat tcatgaaccc aagctgggcg
     4801 acggctgtgt cgtagcgaca gccgtctagt ataactacaa tcagtttgtc aagtctactt
     4861 tgcataggtc agtacctccg cctgccaaag ctctggaagc tctacagagg tcttgttcca
     4921 agcttcattg tcttcaactg gcaccgccgc ttcataagca tcctccggca gtaatttttc
     4981 agctacttct ttaggcagct ctaccttgcg aataggtcgc gcatagccct ttgccaaatt
     5041 gatttgccct tcatcagata aaatatattc ccgtgctagc tttgccgcat tgggatgagg
     5101 ggcattttta ttgattaagg ttgtataccc gctaatgatg gagccgtctg ttggcacagt
     5161 tacctcaaaa cgctcggagt caatttggtc acgataattt agtccattga agtcccaaac
     5221 aactgccaca tcgatttcac ctttttcaag atttgctacg ctggcatcaa cggtggacaa
     5281 acggcctttt tctgccattt ttttgtagta attcagacct ggctgaatat ccttttcgtc
     5341 acctccgttg gcaatcgccg cagccaatac agcaaattgt gcttggttgg caatcgtcac
     5401 atcccccacg ctaacttgat agtcaccctc ttgcagctcc ttccagctag tcggtgcttg
     5461 cttcacattc tctttatcgg tgataaagga aatggttccc gtatagctta acaaccaatc
     5521 cccatcatca tcctttgccc aatccggaat ttcctcccaa tagttggtct tataaggtaa
     5581 ggttaactct ttgctttcag ccaacggacc gaagctgata ccgacatcac caatatcggc
     5641 ggttccattt tttccttctg attcaaactt agccaattct tctgcactgg acatatccgt
     5701 atccacgtga cttaagccgt attctttttc aaggtcgttc caagtgccta cccaatttgc
     5761 ccaagtatcc ggcattccca agctggcaat ctctccttct tccttggctt tttctataat
     5821 cgtatctaaa gagtcgtctg ccgcagccga ttgcttcgtt gcttcacctg agccgcagcc
     5881 tgtcagcagg atacttactg ttgctgcaca aaataaaccg atagtcatgt tacgaatttt
     5941 ttcattcatt ttcattcatt cttctcccct ttcgataatt ccattctaac aaagctttgt
     6001 aatgagagaa tcagtttctt gaaaaggttt catgaacaat gtaaaggttg tatatggttt
     6061 gtaaatttcg acgaattccc gttaaccttc taaagctttt gcggtatgat agtagaaaaa
     6121 aggaggctcc ctatgcagta ttatgatcag catctccaca cgtatttttc ctttgattca
     6181 gaggaaaaat ttgaaaacta tttagcctat cagccggagt ttttcgtttc tacggatcac
     6241 tttgatttaa acaatccatc taccggaaaa gatgacattc cggattatcc agcctatata
     6301 aacaaactgg actctttaaa agaaacctat tccaccaaat ttctgcgagg tatcgagatt
     6361 ggcgttgttc caggacaaga agagcggatc gtggattacc tcgctcagca tccttatgat
     6421 ttaaagctgg taagtattca tcaaaatggc cgctttgatt atatggacga tgtggtttta
     6481 accaaagatc ccattcaaac taccaaggaa tattttgatc agatgttgca ggtcttgacg
     6541 gattttccag aaggagatat tttgactcac tttgattatg gattacgccg cttcaccttc
     6601 acagcggaag aattcgcgga gcattttgag gctcagctgc taacgatttt caaaaaggtt
     6661 gttgaacgag aattggcgat ggagttaaat gccaagagct tcttgaaata cggcaacgaa
     6721 gacctttacc gttatgccgt tcctttgtat caaagtgtcg gcggaaagct ctttacctta
     6781 ggctcggatg ctcatgttgc cgaagattat cagctgggct ttaaaaaaat gagtcagctg
     6841 ttggcagact gtcaggtaga aaagttgcta gtcattcaag gaaaagaacg ccgcctgacg
     6901 ccgctgccga ttttgtgatt cctatataat agaagaaagt ggtgaaaaaa atgagcaacc
     6961 tgtgggacta caatcaagag gcacccatcc attatttaat tgcgcgacat tgggatgcgt
     7021 tgaaaattga ggcggtttgc cgatccttgt tagcagccgt tccaaaacaa caactggaaa
     7081 attttttggt ggctgacagc ttgcagcggg aaaaagttca ggcttatttt gcagccttta
     7141 aagatcagcc tttggaatat ttgcatgcgc agtttcatct tttttatcaa gtagccgccc
     7201 cagatgacta caacgattta cgaggacagc tgcaattgac ctttcaagcg gatgaaaccg
     7261 cctataccgt tttattggga atggctcgcc ttggtgatca ggcgaaggtc gagtggcgga
     7321 ttttcgatat ttgatggcag gatcaaggcg tttcagttgc aaaccttcct ctctttcaat
     7381 tagaaaaacc gcagcaggaa gaagtttgtg tgctggtcac cgatgctggt gaaattcgtc
     7441 tccgcttatt tcctgaccaa gctcccctgg ctgtagccaa ctggcgctcc ctagctgagc
     7501 aaggcgttta tgacaacaca cctttcggct cgtgtcatta aggaatttgt tattcaaggt
     7561 ggcgctttgg atggttctgg tgatgaagct gtttccagct atgagggctt tttcgctgat
     7621 gaagtgcatc gcggacttta tcattttaat ggcgccttgg ccttagggga ccacggcccc
     7681 cacaccaacg gcaaccagtt ttttattgtg caaaacacaa aggcgcaggc tgacctgcta
     7741 atgtaaagca agcatacttg gagcttggag gactaccgga gctggatggt cgttatactg
     7801 tttttggaca ggtataccaa gggttagaaa tcgtagcagc gatcgcagct cagccgacaa
     7861 acagtgaaga tgtaccgatt gagccagtgt tcattcgaca aattaacttc gaaaaaacat
     7921 cgtaaaaaga cactcactga cttcagcaaa agctctattc ttgagctggc tgcagctagt
     7981 gagtgtcttt catttatcca taagataaag cttggattca ataaataaat aaatgttttg
     8041 gttaaagctg tgaccagcaa atgaggaacc gtgcgagcca gttgatctgc caatgcaaac
     8101 atttctttag gtgaaggcgc tggcgtgtct gccaacggat actccagtcc caatgcttgg
     8161 taaccgtgca taccatagca gtggtacggt agtaattcta acggtacgaa ccgatcaaga
     8221 tctgccacaa aacaagcaat cgtcagcagc tcggtcaggt tcatattgat acctggaacc
     8281 acaggaactc gaattcgcac ttcgcagtta ggatactcaa ttaaaaagcg acgaatattc
     8341 ttcaaaattt gttggttatc taagccggtc cattgtttgt ggagcttgct ggtaaacatt
     8401 tttacatcca caaacaatgt atccacatag ggagctacct tttggatttc attataagca
     8461 ccacaaaagc tggtttccag tacggtattg attccaatgt atttcgattt ttgcaaaatt
     8521 tctttagcaa aatccgcctg aaccagtgcc tcgcctccgc taatggtcac tccgccatcc
     8581 gagtgaaagt aaaaaacagc atccttctca atctcatcca ttacctcttc agcggtcatg
     8641 gtctggccaa atcctttttc aatctgcttg ctttgtgatt cgggaatcga acaccaggca
     8701 cagcgtaatg ggcagccctt taaataaaca acggtccgta aaccagcacc atcatgtaga
     8761 gaaccctgtt ctattcttaa tactgttcct tccatataga tttttgaatt acacactagt
     8821 ttaaactaaa acacacaagg aaaaacttac catttaatca taaaattagc tcatataaat
     8881 atactaggat gtaaaataga ctggtcaaaa tctggtcaaa aaagaggact aatacaggta
     8941 gtcctctttt ttgttatttt aatattctaa atcatttttt attggtttat tcccttattc
     9001 gatgtagact caaatcacta tatcactaga tatcaaacgg cgtcatacgg aatcaaaaac
     9061 atcaaatttg acctgttaaa tggtcattga ataacattca aaatcaataa aacggagtca
     9121 gacacggagt caaaaacgct gagagtaaaa aatatataca gccttgtagg taaagtacca
     9181 cagggctgta tattttttat ttcagattac cttaaatgat tccatttact ttttatctca
     9241 ttttttccta ctagttagta tcaaatactc tcctattttc tcaataaact atttttggat
     9301 acatggtatc tgaaacagct aaaaccttgt cattacagca aaaaatcacg ttatccagac
     9361 tgtagacaaa gtccatttta tggatgatgt ctacagtctt ttcttttata atagatttat
     9421 gaaaacgaat gcaaggagtg atagctat
//
LOCUS       DACENJ010000100         9427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.101, whole genome shotgun sequence.
ACCESSION   DACENJ010000100 DACENJ010000000
VERSION     DACENJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.101"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(259..1023)
                     /locus_tag="IW748_001692"
     CDS             complement(259..1023)
                     /locus_tag="IW748_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1104602.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(1134..2003)
                     /gene="mdcB"
                     /locus_tag="IW748_001693"
     CDS             complement(1134..2003)
                     /gene="mdcB"
                     /locus_tag="IW748_001693"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ1104603.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(2028..3104)
                     /locus_tag="IW748_001694"
     CDS             complement(2028..3104)
                     /locus_tag="IW748_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1104604.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(3128..3721)
                     /locus_tag="IW748_001695"
     CDS             complement(3128..3721)
                     /locus_tag="IW748_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ1104605.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(3725..5401)
                     /gene="mdcD"
                     /locus_tag="IW748_001696"
     CDS             complement(3725..5401)
                     /gene="mdcD"
                     /locus_tag="IW748_001696"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ1104606.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(5391..5690)
                     /locus_tag="IW748_001697"
     CDS             complement(5391..5690)
                     /locus_tag="IW748_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ1104607.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(5792..7438)
                     /gene="mdcA"
                     /locus_tag="IW748_001698"
     CDS             complement(5792..7438)
                     /gene="mdcA"
                     /locus_tag="IW748_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ1104608.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(7445..8356)
                     /locus_tag="IW748_001699"
     CDS             complement(7445..8356)
                     /locus_tag="IW748_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1104609.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            8476..9333
                     /locus_tag="IW748_001700"
     CDS             8476..9333
                     /locus_tag="IW748_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1104610.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
BASE COUNT         2651 a         2049 c         1685 g         3042 t
ORIGIN      
        1 ctaaactaaa atagtgcttc ctatttttcg tttggttgtt cttattatcg cagatgcaag
       61 cttttaaaca ccatttagtg gagaggttgt gacagagatg ttcagccttg agcgataaga
      121 agcgattttg aaaaatcgct tcccatattt tacaaaatcg tgccttattg ccgataggct
      181 atctctggca caccgtttat tcagaaaaag gagctgcgac aaggcttttg tcacaactcc
      241 gttcataaat atttcctgtc aatccagcat aatcggctga ataaaatcgg cttgccatct
      301 tttttctttc tcagaaaaat caaaatacca tatctgagaa aaatactgtc gttgtgtctt
      361 acttcctgag atcaatcgcc catcttttcg atcgactgaa taatcacagc atctgaaatc
      421 aattcggatg gcaaaactat tttcgtggat ctgtcgataa tttcctactt cctgaatagt
      481 tattttttta gtatgatttc ttacaccgtc tgctttgttt ttttgcagaa ctttctgatg
      541 ttcggcaaac agcttgtctg tataacagct tctggctttt tcaggagttt cttcttccca
      601 agcggattgg atcttcatga atatcttttg gatttccaga aactgtgctt tcttttcttt
      661 agccgttcct gacatgcggg acaatatctc cgctttcagc cttttctttt gtcttttatt
      721 cttgacgata gaaaaagcag aaacccctcc tactgacaaa acagctaatg caagaaatcc
      781 cgtttgctca taactagaag gacctgtacc tggaccataa tacccataag aatctctgcc
      841 gtaagaacga ctgccaaaat aactgccccc atatgaacga ccaccaaatg aacctctgcc
      901 cgaacttcga ccgctagatg tacctccacc atgaccaccc gcgacagcga atccttgggt
      961 cggcagagca aacaaacata ggcccactaa caaaatgatt gctaaataat tgattttttt
     1021 cattgtcttc ctcgttatct tttattacaa aaaactattt ttatgatagc cttgctaaaa
     1081 ggaagagtca ataaccagat gcataaactg tttttttagc aattttgtct tatttagtca
     1141 ttgagtaaca tcggacaaat gtatgtgtcg gcagtccata attcctctat cttcagcatg
     1201 aacaagcttg cagacaagag atctgcgctt cctccaggag agatttggtt ttcctgacaa
     1261 aagtgcaaca gtgctgtaaa tggacgattt ggtaaagcac tttgactagc agcatgagca
     1321 agtttttgca tttccactaa cgttttctga tcactgcgat gcaatacgca tgtgtcatct
     1381 agcgaactca tcaaaactaa caacatatgc atttgtttct gtacatatga atcattatct
     1441 agctccttag ctgcttgaat tgcaagcctg atatgcggat accctaatat cgcttcttca
     1501 tatgcaccat ttacgccata tttttgcttc accctatgac catgagtggt ttttttatga
     1561 cgatattttg tatcaggaaa agcagcaagc ttgctgacat catcaagtat tttcgataaa
     1621 gaaaaaggct gttccatact gattttttgt gcaatgacac tgatcaatag ccccattgcc
     1681 caaatcgctc ctttatgggt attgatgcca tttgtagcaa tcagcattgc cttttccgct
     1741 tttcgcccaa tctcagcaat tctttctctt aactcctgat caatagcatg atgccatgct
     1801 atttgagcca tttcttcaaa aaaaggagtt agcgtcttag cagacttaag aaataaagca
     1861 tagtccatat cggaatgtgc acccgcatcc agtgcatcca ctaatccagg ttttggactt
     1921 aatgttactt ctgcgatcaa tgcttcctct accaacgcag cgagttgctg agtcactgct
     1981 ttttttcgtt cgtcatttat catgacactc actccttctt cctactatta cttgctgaaa
     2041 ccttttttga tagctaccca aatgatatga agtaaccagc ttaaagcaaa tgcacaactt
     2101 gccgtcatta gataagctac aagaatcatg actcctgaag gtaactgcag ccaactatct
     2161 ttaaacaata ataacagtac ttggaaaaca aatacattgg ctaaatacgt acgataagca
     2221 taaacagaca accatttgaa caatggcaat tttggcgaat cgtttacaat cattttggaa
     2281 gcgcctatga agacagcaaa gatgatgact aaactgtata aactttggat cgtattcaag
     2341 taaggtgcat tcgcaaagct caagtctcct ggatgtgcga gcaatgaacg gacagaaaag
     2401 aacgcgataa ctaaggcaac aggtaagaaa agataacgta ctctgtccaa atattgatag
     2461 actttttcgt gataaacaaa tgcggctacc ccataaatca tgaaactagc aacaaaccga
     2521 tctaataagt accagctgtc atgataaggc ccagtcaata cttgtgtttg atagaacaga
     2581 taccatgcga catataaaat cgttgaacca atcaatactg gccatacata ttttttatta
     2641 gcaaatactt tgtaaccgag ccaaacaaaa aacggcatga acaattggat ctgcagcatc
     2701 ataacggtat accataaatg aggcgctcca tcacctgtga catatttcag taaaaagctt
     2761 gcagtatccg tatacggagt ttgttgttgg actgctggaa atagccaaag ataagccgtc
     2821 gtccatagaa gatacggaac gactagttca aaaaactttt ctttcaaaaa ttcccaataa
     2881 gacaaatgct gacttgtttt taccatatta taaacaattg caaaaataaa cattggtgct
     2941 gtgtatttaa cgatgacgta aaaggtacca agaaaagctg tatttgttgc gctgtggctg
     3001 tttgccaaag caaagctcaa tatcgtttgc atcataacag ccgttacagc tgatactttc
     3061 tggaagtcac ttacgttgat tttgattttt ttgttttcaa acatctcttt ctctctcttt
     3121 ttctttatta ccaaagttct ttggttaatt taggtccttc gactgttttc aatagaattt
     3181 ttttctctgg ctctctcaaa tactctttta gtgaaaaacc gccttgccga gtcactactt
     3241 gggtatcgaa ttgctcaaaa aaagcaggat gcgattgaat tacctgaatt ggcaattcaa
     3301 tcggtgaatt tgcgtacagc agcaaatcaa aatcactggt ttcttttact gccggaatcc
     3361 cagtcgataa ttcaaatcct aagctgccgc cgattcccca tttacatcct gataatatgt
     3421 ttctagcagc atgaaattgc tgataaacag gataatccat gattttctct tgtctatacg
     3481 attcttgttc gatgatgtcc cacggatgaa tgacttgttt gacggcatgt tttgagattt
     3541 ctgttgacca tctctgattg cgggcaggtc cacgtaaacc aatcggtagt atttctgcgt
     3601 gatccgtcga cgcacgacga actgttccga acatcttttc agttagaacg acccattcag
     3661 gcaaggtgaa cccttctatt tcatgtttat caaaaaggac aatatcatgt gcttgaatca
     3721 ttttttatgc atcccattct tcgtccattt tggcacggac tttatttgtt tcgacacgtc
     3781 cgaatgtttt ggctgtttcg tttgtgtatc gatttgtcag catcgtgtca tttgtattgc
     3841 ggacatcttg aatggcttca ttgattgctt cgatgacttt gctgacattt tgttctgaag
     3901 cagcttggtc ggtgatatct tctaggaaat gatagatcgc tcctagtttt gtataattat
     3961 tgatatcgta agcgatagct ggaacttttt cagcagctgc ttcgatttca gcaaccgaac
     4021 gttttgtcac acgtgcagca ctttctttag acatcgcttg gacggtaatt tctttattgt
     4081 ttaaagcaat caagcggctt gattgtaatc catgtgctaa aaatgcacct gaaatagcat
     4141 ttcctgcaat caatccaata actggatgtc cagcttgacg gagttttgca taagcagcag
     4201 cactattagc caaagccaca tggataccga tcaattcttc gttatagccg tatgcttggc
     4261 ttggtacatc gatcacaacg atgattggcc gtttcacttt tttgtcttta tcttcttcgt
     4321 aaacgtgatc caaaatttgt cccatgcgga aaccttcaac taaaccaact tcaccgtttc
     4381 gtacacggta catcgggttt ttaggatcag gtacgattga tgtcacgatg gcttcttcta
     4441 tgaatttact tgatttacca taatacacag tactaataga agaagttgga tttttcatgc
     4501 cagttagtgc cgcaaaccaa tcgtaaccaa cacttgctgc agctggttca tttccttctg
     4561 ctacttctgg taagtcaatt gattttgctt gatggttagc aattgcttca ttgtaatcgt
     4621 caatggccat tggttttgaa agatctaaag aatcaagtaa tgataaataa aatgctgagc
     4681 tttcactgcg atgcgtgtct tttcgttcgt ccattgctgc gatgacagca gctttgattg
     4741 cttctacact atcagtcact aattcatcaa tgatttttga ttctacccgt tgacgagatc
     4801 ctaatgtatt ccaaatcaag tctttgtcac tagaatcaaa ttctcgaacg cctgcttctt
     4861 gttcaataac ttctggtcca ttcaaaccga ctctcgcttt ttttgttgcg attaaataac
     4921 tcattaatgc tgaagtaatc gacataccgc caaatgatcc tacacgtcct ggaatcaaac
     4981 cgatgactgg tacatattgt tttagagcaa tgattacatt tccgatttct gaaatagata
     5041 acagtccgta gtttgcttct tgcaaacgaa caccacctgt atctagtaca atgactggaa
     5101 agatctcatt tcctttttta ttttcttcta acacatgttc aagtgcagca acgatttttg
     5161 caccggaaac ttcaccgata ccgccacctt ggaattttcc ttccatagcg attactactg
     5221 cttgtttgcc attgatcgtg ccacggctga caacgatccc gtcatcactt tcagggacga
     5281 ttccttgtgc tggtaaactt ggcgcgatga tttgatcgaa tggatcaatc aattcacgtc
     5341 cattgtcaag tagagcgttc actcgttctc tggcgtttaa ttctacaaaa ctatttttca
     5401 tctgaaagtt cctccattgc ttgcgtcaat cgtaggtaca caactcctgg tgtagcaccg
     5461 aagtcattga tcacgatatc tgctgaaatt ggataacgat caaaaaatct cgttaataca
     5521 ttgttccata cttcgccgaa gccatcgctt cctgtacaaa tttttacagt agattctgtt
     5581 ttttctgttg gaaacatcaa gatttccaaa tctcctgaac cgaccactcc tacatggatt
     5641 ttcttattga tcggctgttt cgttggatat ttataagcta attgttccat ggatctctcc
     5701 cctttttgta aaacagagca tacacgttct gcttattcgt ctttcttctt ccatctgggt
     5761 aaatggcgac aaaaggtttt tctcgccaat tttatgacca agttctgaat ttcattggtg
     5821 gttcatacag gccatctgac cattcgatca aatcatcgat ggtttgtgca gctaataatg
     5881 aacgtttcgc ttcactacgt ttgatgccta gatcttctgg taatttcacg atgccggctg
     5941 cacgcaagct atctagtgat tctttcgtgc tagtcattcc aagaggtgta acaccgccaa
     6001 ttgctgcaat tgctgcttgt cgttcttctt ttgtttttgc tttgtacaaa taagcaattc
     6061 cttcttctgt cacgatatgt gtagtatctt cgctataaat catcactggt gcattggcta
     6121 agcctgcttg tttttgaaca gctagtgcat ctaattcttc gacaaagact ggttttttat
     6181 ttgaaccata tgtttcaacc atttgaacaa ctaatttttg gcctttccct aatggatcat
     6241 cattgtcgcg aagtgattgc catgctggtg tagaatgacg acgaccgcca ggattatgtc
     6301 ccatgttagg cgcaccgcca aaaccagata aacgtccttt tgttacggta gaagagtttc
     6361 cttcgtaatc taattgcaag gttgaaccga taaacatatc gatggcatat tgtcctgcag
     6421 cttgtgataa tgttcggtta ctgcgcatcg tgccgtcttt accgacaaaa aagacatctg
     6481 ggcgagcagc aatatatttt tccataccaa cttcgccacc aaaactatgg atgctttcaa
     6541 cccaacctga ttcgatggct ggaatcatcg ttggatgtgg gttcaatacc cagttcttcg
     6601 cgattttccc ttttaaccct agtgattcac cataagttgg cagtaacaat tcgatagctg
     6661 ctgtattgaa tccaataccg tgattcaaag attgtacgcc gtgttctgcg tagattcctt
     6721 tgattgccat cattgccatt agaatttgca attctgtgat gttttgagga tctcgtgtga
     6781 ataaagcttc taattgataa ggtttatctg ctttcacgac aacgtctacc cagtcaccag
     6841 ggatatctac acgtggtaaa gaatcgacaa cttcgttgac ttgaacgatc acgataccat
     6901 ctttgaaagc tgcagcttcg atgattgttg gtgtttcttc cgtattgaaa cctgtgtaca
     6961 agttgccgtc tttatctgct ttatctgcag ctactaaaac aacatttgga atcaaatcga
     7021 tgaataagcg tccataaagt tccaaataag tatggatatc acctaatttc attgtgccgt
     7081 cttcaatcat ttgggcgaca cgcaagcttt gtgcaccagc ataagagaaa tcgatcttgc
     7141 ttgcgattcc tttttcaaaa atattcaaat gttctggtcg tgagatacta gacatgatca
     7201 aatgcagatc attcactttt tctgcatcta ctttttcaag actttctgaa agaaaacttg
     7261 cttgtttttg attgtcgcct tctaatacaa ctttgtcgcc gggagcaatc agtttttcca
     7321 ttacttctac gattttttca gtttctacat acttgccatt gagcaagttg ctgatttttt
     7381 ccaatcgctg cgcttttttt tcgcgtttag aagcccagtt gcggtttggt ttttccataa
     7441 ttagtcatct ccatttttga tatagaaacg tggcgtcatc aataccatgt tggtctagat
     7501 tgatcgtacg gatcccttta tctacaccaa attttgtatg aatcagcttt gtcaaagtat
     7561 tgccgggggg aaattcaatc gtcagatcca ttcctaattc ttttgccaag tccatcattt
     7621 ggttccactg cacagggaaa gctaggtttt ccaacaaatc tttacgaatc tcttctgcgt
     7681 cctgagtggc tcttccatcc gtatttttta tatacaaaca tctagcatca gatagatgga
     7741 cagactgcat atacggcttg aatttcttga ccgtatcatt catcaagaga cagtgagatg
     7801 gcacagggac tttcagcagc ttcgctgttt gcgcttggtg ccgtttggct tctgccaacg
     7861 taacggataa accttcgata ctcccagata acgtatgttg catcggacaa ttttcatttg
     7921 acagataaac tggatggtca ggatcaaatg tttttgctac aatttcctca gcttcgcttc
     7981 tcgtcacacc tactactacg cccattgcat aaccgcttgg ataagcttct ttcattaaga
     8041 ctgcgcggtg atagactagt tccgctgcat tctcaaatct caaagatcga caagcaacag
     8101 ctgcacaaaa agcaccaatc gaatgtcctg ctgcaatatc aggctgaata ccgttttttt
     8161 ccatttcatc taaataaaat actgcttttg tcagtaatgc tagctggata aaaaccgtat
     8221 cttgatagtt ttcttcttta tcaactaacc gataccccgt cacattttct aaacgttcca
     8281 tgtacatttc agggatatcc gctaacatcc ctaacttctg actgccttgt ccaggaaata
     8341 agaaacctgt actcatgagc ctcgcctcct cagcacttac tttttccact gctatcttag
     8401 tcaatttctg ctataataaa aaatgccaaa aaaagaaaaa accataacaa aaaggtatgg
     8461 atgggatgga taattatgaa attgcttcat ctgcagtatt ttaaaaaagt attcgaaaca
     8521 aaaaatgtta cccaagcagc caaagaatta tttatttctc aacctgcctt gagccgtgcg
     8581 atcaaacatt tggaaaacga attaggtgtc cctttgtttt accatagcgg cagaaacatt
     8641 gagacgaccg tttatgcaga agaattttat ccatatgcag tcaagacatt agacacatta
     8701 gaagaaggaa tcaaattatt gtcttcggtc aatgagaaga tcgttacttc cgtcatattg
     8761 tatttagagg tagcctctgt ttctattccg aacttggtcc gaatttttat ggaaaagcat
     8821 cctgatatcc aattaacgat catgcaacat catttaccag atgatattac cgatcctgta
     8881 ctgtacatca cttcggagat gaagcctggc atgaccaata ttcctatcat gaaagaacct
     8941 gtttatgtcg ccattcctaa gaaacatcct cttgctaaaa aagaacatct gcaattagaa
     9001 gatatccaac gcacccccat cttgatgctg tcaaagagca acgccttccg tcatacactg
     9061 gatgcagcac ttgaagcgaa aagtatcgaa ttgacgatca gttcgacgac agatgatcct
     9121 gcgacattac gttctattgt caagcaagga ttagctgtta gctttttccc aaaagtttct
     9181 tggtcttacg ataaaaatga tcctttcgtc ttgcgggaaa tcaaagattt gcccttgaca
     9241 cgaacaattt atttatcttc tagcttcaca gaagatcatc ctttgatcaa aatgattgca
     9301 aaaacgatgc gggaatttta tttaggaaaa taaaaaaaaa agagctggga caaaattctt
     9361 gtcgcagctc ttttttcgaa caaacggtgt tccagaagta atttcttaga aaataagccg
     9421 aaatttc
//
LOCUS       DACENJ010000101         9279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.103, whole genome shotgun sequence.
ACCESSION   DACENJ010000101 DACENJ010000000
VERSION     DACENJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.103"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..264)
                     /locus_tag="IW748_001701"
     CDS             complement(<1..264)
                     /locus_tag="IW748_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1104611.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAP"
     gene            589..1005
                     /locus_tag="IW748_001702"
     CDS             589..1005
                     /locus_tag="IW748_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1104612.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1002..1472
                     /locus_tag="IW748_001703"
     CDS             1002..1472
                     /locus_tag="IW748_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1104613.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1486..2259
                     /locus_tag="IW748_001704"
     CDS             1486..2259
                     /locus_tag="IW748_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1104614.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2273..3085
                     /locus_tag="IW748_001705"
     CDS             2273..3085
                     /locus_tag="IW748_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1104615.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3139..4104
                     /locus_tag="IW748_001706"
     CDS             3139..4104
                     /locus_tag="IW748_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1104616.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4220..6943
                     /locus_tag="IW748_001707"
     CDS             4220..6943
                     /locus_tag="IW748_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1104617.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANCMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            6956..7588
                     /locus_tag="IW748_001708"
     CDS             6956..7588
                     /locus_tag="IW748_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1104618.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8487..>9279
                     /locus_tag="IW748_001709"
     CDS             8487..>9279
                     /locus_tag="IW748_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1104619.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3139 a         1411 c         1816 g         2913 t
ORIGIN      
        1 aggggcgttt cctgatttta aatatcttaa cccgccggtg tggtcagaat gaccgtgtga
       61 aataatcact gcctccagat cattcaaagt ttcccccatt ttagtcgcat tatagactaa
      121 ttttttatta gcgccagcat cgaagagata tttcttccct gagtagttaa ttaagagaga
      181 taacccatgt ccacttttaa agttcaaatt tattcttgag ttttcaaata aaaccttgat
      241 ttccactata acatctcctt tcacatgaac gtgtattcat atattgtagc acattaagtt
      301 ctattttttg ttgtaattac cgaaaaaaat attaatggac gctttttacg ctttatttcg
      361 tatgtggaag aaaatttctt aggatgtggc gggggattgg ggcaaagcta aattccaatt
      421 acagtgatca tttaattaga cacctttaaa tacccacact ttcatcaaca ctataattta
      481 gggaaagaaa agtgttggca tgatatagaa aaacctttaa ttaacagcgt ttcctaaaat
      541 tggcacgcat cttgcttata gaaaagcgta aggataggag gaatgaaaat ggcaactata
      601 attcttgcca gccatgggga atttgctcaa ggattaaagc aaacagcgac aatgattatt
      661 ggagatagtg taccaattca tgccttgtca gcatttagag atgaagatga atctattttg
      721 gtacaaataa aaaaactttt agccacaata gatatagaag aaacctacat tttaactgat
      781 attctaggtg gtagtgtgaa taatgatatg ctcactatta ttaaagagta tgagggattg
      841 caattaatta ctggaatgaa cttaccttta gttatttcta ttgcaaccta taatggaaaa
      901 attggggaaa gtgagctaga aaagcttatt aaagagagtc aacaatcagt gattgattgt
      961 agaaaattat tagtagaaca acttactgga ggagatgacc tatgattaag ttagtacgta
     1021 ttgaccatcg tttgttgcac ggacaggtag tattttcttg gagtaaatca ttaaatatca
     1081 atcgtattat tattgccaat aaagaggcag cgaatgatga attcaaaaaa atgtctttga
     1141 atttatctaa accgactggc attaaattaa caattttttc agttgaacag ttactagaaa
     1201 aaatgcctaa aatcgaaaca cttaacgaaa atatcatgct tatttttggt aatacaaaag
     1261 aaattcgtca attttgtgaa gcctatccta aagtacaaga aatcaactat ggagggataa
     1321 tcaaaaaaga aggttccaag caatttagta atgccatttt cttgaatgaa tctgagcaag
     1381 aagatgccaa aaaattaaaa gctcttggca ttaaacaatt tattcaacag gtaccaactt
     1441 ctaaaaaaga agatttgaat acaatgttat aaaggaggaa tgaaaatgct cgttcagtca
     1501 attggtttag ggttaatcgg tgttttttgt attttagact caaggctttt aggaagaatg
     1561 aattttgaac gaccgttgat tgtcagtacg cttgttggtt tggtacttgg agacatggaa
     1621 aaaggattaa ttgttggagc ctctttggaa ttgatgtcct taggattagt aaatatcgga
     1681 gctgctgcac cacctgatat gaatatggca tcaattattg ctacagcctt tgccatttta
     1741 tcaaatgcga atgcagaagc agcattaacg attgccattc caatttcagt gttaggtcaa
     1801 atactaggta tcttaatgcg gacgattttg tcaaatttga ctcacgttgc ggatcgcttt
     1861 atcgaacaag ggaaatttag ccaagcaagg agaattcata tactttatgg ctcgggatta
     1921 tatgctctca tgtattttat tcctattttt ctagcaattt attttggaac gaatcttgtt
     1981 tctcagattg ttgatgcgat tcctgagtgg ctaacaaatg gcttaacggt tgcaagtaaa
     2041 attttaccag catatgggtt tgcgttgtta ttacaaacga tgttaacgaa aaaaatggtg
     2101 ccatttttat tacttggatt tttaattact gcttattctg gattgggaat cattggtatt
     2161 gcagccttcg catgtattgt tgcgtttgtc atggttcaag ttcaaggtaa aaactcaaat
     2221 ggtaaccaaa atgagccaga tccattagat gacttataag gaggtagatt aagtgaaaga
     2281 gaaagatcaa aatgcatgga aatatcggca gttcttatgg cgttcatggg caattcaagc
     2341 ctcatggaac tatgaacgac aaatgaacat gggttttatg tacggaattg caccaattct
     2401 agataacatt tacaagaatc cagaagatga agcaatgaag aaagaagctt acgagcggca
     2461 tatgatgtac tacaactgta cccctcaaac aagtgcattt gtgttagggt taactgcttc
     2521 tatggaggag caatactacg aagaccaaga aaactttaat ccggaatcaa tcagtgcagt
     2581 gaagacttct ctgatgggac cgctatctgg tgttggtgat tctttctttc aaggaacgat
     2641 tcgggtatta gcctttggac taggaattaa tttagcccaa caaggtagta ttttaggtcc
     2701 aattctagca atgttaattt cctttgtacc ttccttttta gttacttact atggtgggaa
     2761 aattggctat aacatgggga ataaatactt gtctaaactg tatcaagaag gtttgatgga
     2821 taaagtaatg tatgtttgct ccattgtggg gttgatggtc attggttcga tgattgccag
     2881 catgatcggg attacaacac caattcaaat gggtaaagct tttgtcttac aagatgtatt
     2941 agatagtatt atgccacaat ttttaccatt aggattaacc ttcttcatgt actggttatt
     3001 gcagaaaaag gtcaaaacag gatggatgtt ggcaatctgt ttagtcggag gaattatttt
     3061 tagtttgtta ggtatcttac aataagggaa taaccaataa atttggttga ataatataaa
     3121 taaggaggaa ttcaattgat gaattcaaaa gaaattgtta aagaaatcgt agcgaaaaaa
     3181 gaggtaaaat caatcgtatt tgttggctgt ggcgcttcaa aagcggacct ttatcctgca
     3241 aagtattttc tagaccaaaa tgcgaagcag ttacggatta gtcattacac agccagcgaa
     3301 tttaactatg caacaccagc atctgtagat gagtcaactg tcgttatttc agcttcttta
     3361 ggaggtacga ctccagaaac agttgaagct aacgcagtag ctaaagaaaa aggtgctaca
     3421 gttattagtc taacacgaag cattgactca ccgttaacaa aagatgcaga ttatgtaatt
     3481 tatcatcgat ttgcagagaa ctatgctgct aaattagaaa aaacaggcta ctgtttagaa
     3541 ttggcagtag aacttttaca tcaagtagaa ggttatgacc attatgaagc aatgcaagaa
     3601 ggatttgcta ctatctatga tcttgcccaa aaaagtgcag aatcagctcg taaagatgcg
     3661 aagaaatttg ctgaacgctt taaagatgaa aaagttattt atgtaatgag tagtggtgct
     3721 acacatgaag tagcatactc aacatcaatt tgcttgatga tggaaatgca atggattaat
     3781 tctggtacct tccattcagg agaatttttc cacggaccat tcgaaattgt ggataaagac
     3841 gtaccgttta tattattaat gaatgatgga aaaactagaa atatagatgc tcgagcatta
     3901 actttccttg aacgctttga tacgaaaaca gaagttgtag atgccttgga ttatggcttg
     3961 tcttcacata ttgctaaaga agtcttagac tactttaacc cattcgtgat tactgcagta
     4021 tttagagttt atgcagaaga attgtcagaa gcaagagaac atccattaac taagagacgt
     4081 tatatgtgga aactaactta ttaaaaaata gatcaaacta aataaggtgg cttgagaaat
     4141 taagccacct tgttttcttc aatgtgattt tatacatatg gtaaaataac tagtacatat
     4201 tgtgaaagca ggtggcaaga tgcagcgatt aaatgactat ctttatcaac taacattgct
     4261 tttgaaatct acgccttcag ataattattc agctcgcagt atttcgcaag ctttacagtt
     4321 gaatagaagt acaatcagta gctacttaaa tgaaggtgtt cgtgaagggc ttttcataaa
     4381 agtaaaaagt tacccggttt tatttttaca tagaacagct cttgaagaac tacatatcac
     4441 actgaataac tccgaaatag aatcaattga aagcttgtta acaatttctc aaaaacccgc
     4501 attagatcaa gtaattggct ctaaaggaag tttgaaagaa gcaattgatc aaattaaaac
     4561 ggcagtctta tatcctggaa aagggttgcc tttattattg atcggcgcta gtggatcagg
     4621 gaaaaccttt ctagcgaata aaatatatga atatgcagta gaagaaaagg tcattcacac
     4681 atcggcacct ttcatagatt ataattgtgc acaatatgtg agcaatccag agttactatc
     4741 ctctgcttta tttggctaca cgaaaggagc atttaccgga gcaagccagg agcatactgg
     4801 tttgctagaa aaagccgatg gaggagtatt gtttttagat gaagtgcatc gcttatctga
     4861 agaagggcaa gagaaattat ttacatttat ggatacgggt gaattctctc ctatggggga
     4921 taatagtatt cggaaaaaag ccgatgttcg attagtcttt gcaacaacag aaaatatata
     4981 tacgactttc ttaccaactt ttcttaggag attgccagtt attgttaatc tacctagatt
     5041 tcaacaacgt ccgtcgtttg aacgtctgtc attgattgat gaattttttg tttctgagag
     5101 taaaatttta gctaaggaac tatctgtttc tgatgcattg attcatttct taatgaatag
     5161 tgattttgaa ggtaacgtgg ggaatattaa aaatattatc aagtatgctt gcggaaacgc
     5221 atatatccac cagaagagtc agcaaacaat tcaagttaaa ttattggata tgcctttaga
     5281 gtacagttat aaattcaagg agcaactaaa taaacccaga aagaaaagag cagatcgaat
     5341 ctactatcca gaagaaacac gacaagtgca actagaaaga aacaatcagc tttttgttca
     5401 attttttgac caattactta ctggattctc tgaagtgatt gaaaataaca aggatattaa
     5461 actattttta gaagaaatgg ttaccaaggt aacacaagta atggatacgc ttatttttca
     5521 agaggattac gaaaaagagc agtctttata cgccattcta aactatcata ttcgtcaaag
     5581 tattgatttt atgcgagaaa gctacgggtt tgaacaagat ggtaaccggg tagtcgcttt
     5641 ggccaactat ctatatgtca aggataatca agtgattttt gaaaatagtc ttcaagaaaa
     5701 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
     5761 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
     5821 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
     5881 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
     5941 ctgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
     6001 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
     6061 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
     6121 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
     6181 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
     6241 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
     6301 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
     6361 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
     6421 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
     6481 taatcagatt ccatatattg gattagatcg cctgattaat ggggaagcaa ttggcgaatt
     6541 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
     6601 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
     6661 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     6721 taataatcag ctgtttttgt tgtaccttca ctgtagttgt atgattgaac gaattgtacg
     6781 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     6841 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     6901 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     6961 aatgggagta atttttgata tggatggggt actcatagac agcgagacat tttattttga
     7021 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     7081 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     7141 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     7201 taaagattta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     7261 attggcactg gcatcttctt ctccagttgt tgagatagag agtatgctca ttcaatgtga
     7321 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     7381 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     7441 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg tggcactaaa
     7501 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     7561 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     7621 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     7681 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     7741 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     7801 acattctatc tatttcagaa cgtaaccttc cccagttcat tgggattcat tagcatacaa
     7861 ccaacatttc aaaaagaaat tgcactacac cattattagc tattttgact aaccccaaaa
     7921 aacttgctat aattcaggtg taggtaaaag gtgctggtat caccttttac gaaactgctt
     7981 cggcagtggt aatgagtgtg gcttaaagga ctagtccttt aagcttttta ctttgttcgc
     8041 aatctctaag atcaagttaa tgatcgataa gattgctaga atattttgca acattctatt
     8101 tcagaacgta acctttccca ttccactgtg attcattggc aaccctcctc acaaccaacg
     8161 tttcaaaaga aattgtaaag ttgttggggt accacgaccg aacaatacta cacctgaatg
     8221 atagtaagtt gtctcattaa gtatagcata tcctcttttt gggtaaatag aaatcggtta
     8281 cattttttat ccaagaccaa gttttttcat ttggtcttat ttgctatacc caaacaattc
     8341 cgcagtatag tcgacggtat tgattttatc aggtgagtaa tagaaacttg ttttccttcc
     8401 ccaaaacatt acctataaag gagaaaaaat ggtatcgtaa ttatataggt ataggatgaa
     8461 atttttttct aagaggaggg ttgttgatga aaaatttaca gttaaaattt attgttgatc
     8521 catcgattgt acgctggttt cggattatca atcaatttga gcgcaatcaa cttgccacaa
     8581 aaggagaatt agcaaagagt aatcatgtat ctgaacgaac cattcaaaca gatgtcaata
     8641 aaatgaagga ctacttttct gaaagtgttc aatttgtttc aacgaaaagt gggtattctt
     8701 ttcagaagga aagaccggtt ctttttctca agcagaagga gcaactactg gagaacgaaa
     8761 ttttatttga gttgttaggg aatgtctttt atggcgaatt ggaagatatg acggagctaa
     8821 ttgatcgttt tcatttatct gaagcgactt ttcgtcggta tttgaagcag attcaaccag
     8881 ttttggaaga atacggctta gaattgagtc tttatcctct tgatttaaaa ggagaagagg
     8941 caaatattcg caagtttttt aaagacttct attatgaagg ggagatgacg ccccatactc
     9001 ttgttccacc aagggatctt catgaacttg tgcaagctac attctatgat aaatttgagt
     9061 atttctcgat tggaacaggg acttctcctg ctgaatttta ttatagtttg tatattgcga
     9121 ttgagcgtgt gagacaagga aaaacgatag ccattcctca aggtctgatt gagcgagtaa
     9181 ttgctagtga gaattttcgc ttaatgtatt ccttgaaaga agcgattcaa acaaattatc
     9241 aagttgcttt atcagaagag gagttttgtt ggttgtatt
//
LOCUS       DACENJ010000102         9252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.104, whole genome shotgun sequence.
ACCESSION   DACENJ010000102 DACENJ010000000
VERSION     DACENJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.104"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            10..537
                     /locus_tag="IW748_001710"
     CDS             10..537
                     /locus_tag="IW748_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1104620.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            600..1970
                     /gene="radA"
                     /locus_tag="IW748_001711"
     CDS             600..1970
                     /gene="radA"
                     /locus_tag="IW748_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1104621.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            2004..3161
                     /locus_tag="IW748_001712"
     CDS             2004..3161
                     /locus_tag="IW748_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1104622.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            3331..4791
                     /locus_tag="IW748_001713"
     CDS             3331..4791
                     /locus_tag="IW748_001713"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1104623.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            5113..5649
                     /gene="cysE"
                     /locus_tag="IW748_001714"
     CDS             5113..5649
                     /gene="cysE"
                     /locus_tag="IW748_001714"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1104624.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            5646..7058
                     /locus_tag="IW748_001715"
     CDS             5646..7058
                     /locus_tag="IW748_001715"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1104625.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            7055..7450
                     /locus_tag="IW748_001716"
     CDS             7055..7450
                     /locus_tag="IW748_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1104626.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            7443..8303
                     /gene="rlmB"
                     /locus_tag="IW748_001717"
     CDS             7443..8303
                     /gene="rlmB"
                     /locus_tag="IW748_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1104627.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            8307..8837
                     /locus_tag="IW748_001718"
     CDS             8307..8837
                     /locus_tag="IW748_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1104628.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            8914..>9252
                     /locus_tag="IW748_001719"
     CDS             8914..>9252
                     /locus_tag="IW748_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1104629.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFL"
BASE COUNT         3149 a         1693 c         2096 g         2314 t
ORIGIN      
        1 ggaaaaccgg tgagaaaaag aggatttgaa gttatttcta aatataaaga tcaaaatatc
       61 aatctaccgc agcgcgctac acatcatgct gctggctatg attttgaagc agcagaagag
      121 gtcgtgattc caagtatctg gaaagcaggc tggaaaaagg ggttggaaac agtcgcagat
      181 ggattgaaaa aagatgtatc agaaaatgaa aaacaattga aaccaaccct cgtgcctact
      241 ggaatcaaag cttacatggg agaagatgaa tttttgcagc ttgctaaccg ttcaagcaat
      301 ccgttgaagc ggtttttagt gttgacaaac ggagtaggtg tgattgacag tgattattac
      361 aataatgagg caaatgaagg ccatatcatg ttccaattca ccaactttgg tttcaaggat
      421 gtagtgatca aaaaaggcga gcgtatcggc caagggatct ttttgccgtt tttaaaagca
      481 gatcaagatc aaacgactgc tcaacgtcaa ggcggatttg gttcttccag taaataaaac
      541 gttaaagata cttaagcttg atttaatgtc tcatgattag gataaaagga ggcggtacaa
      601 tggcaaaaaa atcaaaagta caatttgttt gccaaaattg cgggtatgtg tcgcctaagt
      661 tcttgggacg ctgcccaaat tgcggaaaat ggaatacgat ggtcgaagaa atcgaacaag
      721 atacaacgga ccgcagaact cggacaagtt tgacaggaga aaaagcaaaa ccaacaaaaa
      781 tcgcagacgt cgtccctaaa aaagaacctc ggatcaaaac aaaattagaa gaactgaacc
      841 gtgtgttagg cggaggtgtg gtgccagggt ctatggtctt gattggtggc gatccaggta
      901 tcgggaaatc cactttgctg ttacaagttt ctcagcaatt agcagccata ggcggaaaag
      961 tcttatatgt atccggcgaa gaaagcgcag agcagataaa attacgcgcg gaacgcctcg
     1021 gaagtatcaa cacagaattt tatctatacg ctgaaacaga tatgaacgaa atcagtcgtg
     1081 ccatcgaaca tatctcccct gattatgtta tcattgattc catccaaacg atgacgcagc
     1141 cagacatcac aagtgtggca ggaagtgtca gccaagtaag agaaaccact gcagaactac
     1201 tcaaaatcgc taaaacaaat gggatagcca tttttatcgt cggtcatgtt accaaagaag
     1261 gctcgatcgc cggcccaaga atgttagaac atatggtgga tacggtgtta tattttgaag
     1321 gtgaacaaca tcatagtttt cggattttgc gagcagtaaa aaaccgattt ggctcaacga
     1381 atgagattgg gatttttgaa atgcatgagc atggattgga agaagtcgct aatccttcac
     1441 agattttctt agaagaacgc ctagacggtg caactggctc tgctatcgtt gtggcaatgg
     1501 aagggacacg tccaattttg gtagagatcc aagcattagt cacacctact atgttcggaa
     1561 atgccaaacg aacaacaaca ggtcttgatt tcaatcgggt atctctgatc atggcagtgc
     1621 tggaaaaaag agctgggttg cttcttcaga atcaagatgc ttacttaaaa gctgcaggtg
     1681 gggtaaaatt aaatgaacca gcgattgatt tagctatcgc tgtcagtatt gcttcaagct
     1741 ataaagaaaa aggaacacag ccaacggaat gtttcatcgg tgaaattgga ctgactggag
     1801 aaatcagacg tgtcagtcat attgaacaac gagtcaaaga agtccaaaaa ctcggcttca
     1861 caaaagtata cctacctaaa aacaacttag gtaactggga agcgccaaaa ggaatcgaga
     1921 tagttggagt agcaactttg gctgaaacgt tgaagcgtgt gttccgctaa ctaataaatg
     1981 attgtcgaaa cggaggaaaa acgatgcaaa aacgtgtcat tactttgctg atgatcattg
     2041 tcggcgcaag cttgggtatt tcatttttac cctcagcttg gcaggcaatc ggtcaacagt
     2101 caaacggatg gctgaataat acatttacca acagcctgtt aggcgcactt attttctttt
     2161 tattatcttt gctgttggcc aaacatatct cagctattat caaacaagta gaacaaaaac
     2221 taagtgaggt cagcttgact tatcttctct tcggtgcgat cggtgcgatc atcggattga
     2281 ctttaggtgt tgtgatctcg acccctcttt acaatatgga aattccattc gtcaatagtg
     2341 tattgccgat tttactgatg atcatcttag gctatctggg attgagaatg ggaacgacac
     2401 gaatcgaaga atggaagaaa gtttttggtt cacgaagcaa aaaaatcgag caagaaacag
     2461 ctagtcaagt tgagagtcaa ctgttagaac gaaaatcagg agaaaatttc cataaatata
     2521 aaatcttgga tacaagcgtg atcattgacg gtcggattta tgatattaca aagactggat
     2581 tcttagaagg aaccttgatg atccctaatt ttgttcttta cgaactacaa tacatcgcgg
     2641 actcagggga tagtttgaaa cgtgtgcgtg gtcgtcgcgg attagatatc ctaaatgcct
     2701 tgcaaaaaga agagggtata tctgtcgaaa tgtacgatgg agactttgaa gatatcagtg
     2761 aagtagacag caaattgatc aaattggcta agcttttaga tggtgtcatt gtaaccaatg
     2821 actataactt gaataaagtc tctgaattcc aaaatgtacc tgttcttaat atcaatgcgt
     2881 tggcaaatgc cgtaaaacca gtcgtcatcc ctggtgaaac gatgaatgtt ctagtagtaa
     2941 aagccgggac agaacgccaa caaggagtag cctaccttga tgacggcacg atggttgtcg
     3001 tagaagatgg acagcattac atgaatgaac gtatcgaagt cgttgtaaca agtgcccttc
     3061 aaacagctgc tggacgcatg atctttgcta aacctgcaca tgcaggacga ggaatcgaca
     3121 ataaaaaaga gacagagaaa aagactaaac aatcaaaata aaaaagaaat tcagccaact
     3181 ggtaaggaaa ttcaaccagt tggctttact atttacctta gaaaaggtaa aatttgtgct
     3241 gagtggcttt aaataaatga tatttgagaa gccgtaactg acggaatttt tgctgttagt
     3301 caaagtctag tttgaaagaa gaggaagaaa atgacgaaag tacgcgtacg ctatgcacca
     3361 agtccaactg ggcacctgca tattggaaat gcaagaactg cattattcaa ttatttgttt
     3421 gcccgccaca atgatggaga ctttattatt cgtattgagg atactgacca aaaacgtaat
     3481 atcgaagatg gcgaaaaaag ccagttggaa aaccttgctt ggttgggaat gaattgggat
     3541 gaatctcctg aaaatcctgg agaatacggc ccttaccgtc aatctgaacg taaagaaatc
     3601 tatcaaccgc tgattgatca gctgcttgct agcaatcgag catacaaatg ctattgcaca
     3661 gaagaagaat tagaagcaga acgggaagca caacgcgcaa gaggagaaat gcctcattac
     3721 gcaggaacat gtgcgaactt gacacctgaa gaacaagcag aaaaagaagc acaaggtcta
     3781 gaatctgttg tacgtttccg tgtgcctcgc aatacagaat acgcatttac agatatggtg
     3841 aaaggcgcta tttcgttcga atccgataat atcggtggcg actttgtcat acaaaaacgc
     3901 gatggtatgc caacttataa ttttgctgtt gcggtcgatg atcatttgat gaaaatcact
     3961 cacgttttac gtggagatga ccatattgcc aatacaccaa aacaactgat gatctatgaa
     4021 gcatttggct ggacaccacc aaccttcgga catatgacat tgatcatcaa cagtgaaaca
     4081 ggtaaaaaac taagcaaacg tgacgaatca atcttgcaat ttatcgaaca atatcgcgaa
     4141 ttaggctatc taccagatgc aatgttcaac tttattgcat tattgggctg gtcacctgtg
     4201 ggcgaagacg aaatcttcga acaccaagaa ttgatcaaaa tgttcgatcc aaaacgccta
     4261 agtaaatcac ccgcagcttt cgatgcgaaa aaactgcaat gggtaaacaa ccattacatc
     4321 aaagcaatgg atctagatgc atttactgac atgtgtctgc cttatctaat cgctgatgga
     4381 cgtgtagcag aaaacccaga tgaaaagaca atcgaatggg tgaagaagat cgttagcctg
     4441 tatcaacctc aaatgagcta tgctgcagaa atcgttgatc tgtctacgct atttttcaat
     4501 gaacatcctg tattgaatga agcagcaaaa gaagtacttg ctggtgaaac agttcctact
     4561 gttctccacg cattcaaagc acagctagaa caaatggaag tagtagatgc tccaactgtc
     4621 aaagctgcca tcaaagctgt tcaaaaagaa actggcgtaa aaggaaaaaa tcttttcatg
     4681 ccaatccgtg tagctgtatc aggacaaatg catggtccag aattgcctga aacgatcgaa
     4741 ttgcttggtc gtgaaaaatc attagcacat ttaaatcaag tactgaacta aacacgatga
     4801 acagaaaaaa gtaagcagct gatcattaca agagagatac cgggtggtgc aaggtatcaa
     4861 tgaatggttg ccgaaatgcc tctgggagtg gtcttggcga aacacagaaa acaggaaaaa
     4921 ctgttttata ggaaacaaag tagccaagaa cgtataacag gcgttagctg tcagagataa
     4981 tcattgatta tgaagaaaag tggaaccacg cggaaagcgt cttttgataa ggaaacttat
     5041 ttaaaaggcg cttcttttct ttataagaag atgatttcct acataaaatt cttacgaaag
     5101 gagtggaaag tcatgagttg gtttacccga gcgattgatg cagcaaagag aaacgatccg
     5161 gctgcacgtt cgacagctga gatcgttttg acataccctg gttttcatgc cttgttttgg
     5221 catcgtttct ctcatttttt atatcagcac cggctctttt tattggcgaa gatcaatgct
     5281 caattctggc gtttcctaac tggtgttgag atccatccag gagcaacgat tggaactggc
     5341 gtgttcatcg accatggaat gggaatcgtc attggcgaaa ccgcagaaat cgaggacgat
     5401 gtgattttat tccatggcgt aacattaggc ggcacaggaa aagaaacggg taaaagacac
     5461 ccaactgtta aacaaggagc catgctctct gcgaatgcgc agatattagg accggtaacg
     5521 atcggaaaaa acgccaaaat cggtgcaggt gccgttgtgt tgaaagatgt tccagacgac
     5581 gctacagcgg taggagtacc agcaaaagtc gttcgaatca aaggagaaaa agtgaggaga
     5641 tcagaatgat aaaaatctac aatacattga ccagaaaaaa agaagaattc atccctatac
     5701 aaaagggaca tgtcggtatg tatgtctgcg gaccgactgt gtataattat atccatatcg
     5761 gaaatgctcg tagcaccatt gctttcgata caattcgacg ctattttgaa tatcgaggct
     5821 ataaagtcga tttcgtatcg aatttcacgg atgtagacga taaaatcatc cgtacagcaa
     5881 acgaattagg tattacagca ccagaagttg ctgatcgttt catccgagca tttgaagaag
     5941 atacgaaagc gttgaatgtc gagcctgcta cactgcatcc aagagtcatg gatcatatgc
     6001 cagatatttt agcatttatt caagtgttgg ttgaaaaagg ctatgcttat gaatcttcag
     6061 gtgatgtgta ttaccgtacc cgtaaatttg aaaattatgg gaaattaagt gaccaatcca
     6121 tcgatgaact ggaagtcgga gcaagtcagc gtacagggga agaacagcag caaaaagaag
     6181 acccattaga tttcgcttta tggaaacaag caaaacctgg cgagatttct tgggattcac
     6241 catggggcaa aggacgtccc ggttggcata ttgaatgttc cgttatggca acaaaacatt
     6301 taggagaaac gatcgatatc catggtggcg ggcaagattt agaatttcct catcatgaaa
     6361 atgaaatcgc acaaagtgaa gcgaaaacag ggaaaaaatt cgctaattac tggatgcaca
     6421 atggctatgt aacgattggc gaagacgatg aaaaaatgag taaatctcta gggaatttca
     6481 tcactgtaca tgaaatgatc aaaaacgttg atccacaagt cttgcgtttc ttcatggcga
     6541 ccacacaata ccgtcgtccg atccgctaca gtgaaacgac gatgaaagaa gcagccacca
     6601 atttacaacg actgaagaat acgtttgaca atttgaaatt ccgccaagaa actgcctcag
     6661 agaatttggc agatgatgcg gataaaatcg ctttgttaga aaatttagaa cagcgattca
     6721 tcgaagaaat ggacgatgat tttaatgcag caaatgggat cacagtggtc tatgagcttg
     6781 ctaagtggct gaacacttat ctagaccagc cgaaagtttc tgccgctgtc ttggcacaag
     6841 gagaagcttt attctcgcaa tggctttcga tcttcggtat tttatttaca gcagaagagt
     6901 tattagatga agagattgaa caattgatcg aagaacgaaa tcaagcaaga agcaatcgtg
     6961 attttgtccg aagcgacgaa attcgtgatt tgttgaaaga aaaagggatc ttattagaag
     7021 atacaccgca aggaacccga tggagaagag aagcatgaga gattacacac aattgaacgg
     7081 tttagccttg gcttatgttg gagatgcgat ttacgaaatc tatgttcgtg accacctgat
     7141 tgatcaagga gaaacaaaac ccaataagct gcatcgtgcg gcaacgaatt atgtttctgc
     7201 gaatgcacaa gcctttttga ttcagcaaat gctggaagaa catcttttga cagaagaaga
     7261 agaactttat tatcgcagag gaagaaatgc taaaagccat acgagcgcaa aaaatgctga
     7321 tgttacgacg tatcgggtcg caacagggtt cgaagcgctc atgggctatc tgcatttgac
     7381 aaaacaaacc gagcggatgg aagaattgat ccgctggtgt attcagaaag ttggtgaaaa
     7441 aaatgggtaa agaaagaaca aacaaacatc aatcgaaacg tggatttgaa gacaaaaaac
     7501 aaaatagata ttcaaataaa aagcaaactg gacgaaatca gtcagtttct gatcaaaatg
     7561 aagaagcatt agaagataat ttcgtctttg ggcaccatgc cgcagtagaa gcgctacaac
     7621 aagggcgagg gaataaactc tttcttcaag aagattcacg aggagaaaaa gtagaagaac
     7681 tgaaagaaat cgcacgtgaa caagctgtgc cagtcaaatg ggttcctaaa caaaaattag
     7741 aaactttgag cgatcacggc gtgcatcaag gagtcgttct tgcgattaca ccttatgaat
     7801 atttatccct tgaccaactg attgaacgaa caaaagagaa aaaagaagac cccttctttt
     7861 tgattttgga tagcatcgaa gatccacaca attttgggtc gattttacgg acagcagacg
     7921 caagtggcgt cgatggtgtg atcataccaa aacaccgagc agttggaatc acacctatcg
     7981 taacaaagac ttccactggg gcagtcgaac atgtccctgt tgcacgcgtg acgaatttgg
     8041 tacaagctgt gcaacagctc aaagaaacag gattttgggt atttggcaca gatatgaccg
     8101 gcacaaactt ccaaaaatgg aatgccaaag gatcaattgc tttagtcatt ggtaatgaag
     8161 gcaaaggaat gagtccagga ttgaaaaaag aaatggatga attgctgacg atcccgatga
     8221 ccgggcacgt ccagagctta aatgccagtg ttgctgctgg tttgcttatg tatcaggctt
     8281 tttcccaacg tcagggggag tagaccatga aaaaacagct gttgattgta gacggctaca
     8341 acatgattgg ttcgtggccg gaacttgtcc agcttaaaaa gcaggacaaa atggcggatg
     8401 caagagaagc tcttttacat cgtttatcaa attatgcgaa atatgaagga atggaagtca
     8461 tcgttgtctt cgatgcccaa ctagttccgg gcatccaaca aaattataag aaataccagt
     8521 tggatgttgt ttttacgaaa gaagacgaaa cggctgacag ttatatcgag agaatcgctg
     8581 gtcagctgaa tgaccgtttg acacaagtga cagtggcgac aagcgattta gcagaacagt
     8641 ggatgatctt ttcccaagga gcattacgaa cttctgctta tgaattatat aagacgataa
     8701 aaaaaacgga aaaaacaata gcccaagaaa ataaagatat gcattttatt gatttccgac
     8761 gtaattctcc atggaatgaa gagcagttaa aaaaattgtc ccaaaaattg gacgatttaa
     8821 tgagaaaaaa taaataacat atcgttctaa tcagttcgat gaatcattga tgataagatt
     8881 cctccataaa cgaatttatg gaggaattat tttatgtctg aggagttatt gcgcgctgca
     8941 cgtaatggtg acgaagaagc atttgctgcc ctgtatcaaa aatattttcc acttttacta
     9001 cgtttgaaaa acacctatta tttaagaaat tatgtttctg aggattggga acaagaagca
     9061 aggatcgctt tatatcaggc attgagaact tacgaaaaaa gcaaaggtgt tagctttgga
     9121 agttattata aaagcattat aagcaatcaa atctattctc tgttgaggaa gcagaacgcg
     9181 ctgaagcgtc gtgatcaaaa agatgaagtc tcaatcgacc aaaaaataga gacagaagga
     9241 ccagactttt ta
//
LOCUS       DACENJ010000103         9137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.106, whole genome shotgun sequence.
ACCESSION   DACENJ010000103 DACENJ010000000
VERSION     DACENJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.106"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            43..1560
                     /locus_tag="IW748_001720"
     CDS             43..1560
                     /locus_tag="IW748_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1104630.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1550..2821
                     /locus_tag="IW748_001721"
     CDS             1550..2821
                     /locus_tag="IW748_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1104631.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            2996..3892
                     /locus_tag="IW748_001722"
     CDS             2996..3892
                     /locus_tag="IW748_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1104632.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3906..4769
                     /locus_tag="IW748_001723"
     CDS             3906..4769
                     /locus_tag="IW748_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1104633.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4775..5338
                     /locus_tag="IW748_001724"
     CDS             4775..5338
                     /locus_tag="IW748_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1104634.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5348..6871
                     /locus_tag="IW748_001725"
     CDS             5348..6871
                     /locus_tag="IW748_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1104635.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6883..7842
                     /locus_tag="IW748_001726"
     CDS             6883..7842
                     /locus_tag="IW748_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1104636.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            7928..9007
                     /locus_tag="IW748_001727"
     CDS             7928..9007
                     /locus_tag="IW748_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1104637.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
BASE COUNT         2980 a         1442 c         1933 g         2782 t
ORIGIN      
        1 aacgaggtgt attattcatg tataaattaa aggaggtcag ttatggaagc cacaatgttg
       61 attagtagtg tcaagcgtgt aggcaaagtt gatccgcggt tgtacagttc atttattgaa
      121 catatgggac gagcagtata tgaagggatt tatcagccag atcatccatc agctaataaa
      181 agtggatttc gccaagacgt cattcacttg gtaaaagaat tgaacattcc catgattaga
      241 tatcctggtg gaaattttgt gtcaggattt cgttgggaag acagtattgg gcccatagca
      301 aatagaccta gaaggttgga tttagcttgg cggacaattg aaacgaatga agttgggatt
      361 catgaatttt ataactggtg tcagcatgtg ggggccgaaa tcaatatggc tgtcaatctg
      421 ggtactcgtg gagtagatgc agctcgtaat ttagtcgaat attgtaattt tcctaaagga
      481 acttattgga gtgatttaag gcgttcgaat ggtcaagaga gcccttttgg aattaagaca
      541 tggtgtttag ggaatgaaat ggacggtcct tggcagattg gacataaaac agcggaagaa
      601 tatggacggt tagcagagga aacggctaag gcgatgaaac tcgtagatga ttcaattgag
      661 gtggttttat gcggaagttc gaatcggcaa atgcctacat ttggtgattg ggagttaaca
      721 gttcttgatc aagcgtacga tcaaatagat tatttgtctc tgcatcaata ttatggcaac
      781 caaaaaaatg atctttctca ttatcttgca cgttccttag acatggatcg gttcatttct
      841 gaagtgacag cgatgtgcga tacagtcaag gcgaagaaac atagtaagaa gcagatcaac
      901 ctgtcctttg atgaatggaa tatttggtat cattcagcgg aacaagatga gcaagcagcg
      961 ccttggcaga ttgctcctcc tttactagaa gataactata attttgaaga tgcattacta
     1021 ttaggctgtc tattaatcac tttattgaaa catgcagata gagtaaagat tgcttgtctt
     1081 gctcaattgg taaatgttat tgcaccaatc aggacagaaa caaacggaga agcttggaga
     1141 cagacgatct attatccatt tatgcaagct gctgtgtatg gacaaggaga ggtgttaagc
     1201 ccacaaattc gttcagcaaa ctatgcgacg gaagattttg aagaagtgcc gtatttagaa
     1261 agtatagccg taataaatga caaagaactt gtcatatttg cagttaaccg tggagaagaa
     1321 gaaatgaatc taactgttca cttgtctgaa ttatcattag agggagtgat tgatttttct
     1381 gaaatgagtg gatttgatac aaaagcaact aatcttccta aaagtgaaca agtaaaacca
     1441 agtcattcaa caaatgtaaa agctgaagat gggaaaatca attgtttatt gaaaccgcta
     1501 tcttggaatg tggtcagatg tcgtttatca gatgaaggag gaacaaaaaa tgaaaggtaa
     1561 aaaatggtta attggctctt tgttggtagc tgcaacaggt attttaggag cttgcggtgg
     1621 cggaagttca gcagaatctg attctgataa agaagtgact ttgacttttt ggaatgggtt
     1681 tactgcatct gatggtgaaa tattgcaaga aatcgttaat gaatttaata aaacaaatga
     1741 taaaaacatc aaaattgaaa tggatatcat gacttgggcc aatttgaatg aaaaacttcc
     1801 tacagcgatt tcttctaaaa aagcacctga ctttattgca ttaaattatc cagattttgc
     1861 acagtatgta gaaaacggag cggtacaacc attagacgat ttctgggaat atgatggagt
     1921 agataaatct gattttaaag aaacggctat tgaattaggt gaagtagaag gtaaacagta
     1981 ttttgtcccc atgcaggtac aaggcatgta tatgtattgg aataatgatt tgtttaaaca
     2041 agctggatta gatactgaac aaccacctac tacttgggaa gaattgacag aaatggcacc
     2101 aaaattaact gatagtagta aaaatgtatc aggttttgtc ttcaataagg atggaactgc
     2161 accgttatat aactggatgt tagcaaatgg cggtaaatta gtgaacgatg attatactaa
     2221 gtctgaattt gcgtctcctg agaaccttaa aacgttaaaa gcgatccaaa aaatgatcca
     2281 tgtagacaag actggtccag aatcgatttc tggcgccgaa atggacaatt taatgaatgc
     2341 tggtcaacta gccattgaaa tcaatgggcc atggttaaat aacggattga aagcaaacga
     2401 gattgattat aatgtgacta ccttaccaca agggagtgat tcagacaaaa aaacagctat
     2461 tttagatggc gtaggatatg ccattccggc aagtacagat aataataaaa aagaagctgt
     2521 ctatgagttt ttgaaatatt ggaatacaac tgaaattggt aaaaaatgga gtatcgaaaa
     2581 tggttttcct gcctacttgg attcagtagc aaaagatgaa gaagtaaaaa atgatcctat
     2641 tgtatctgaa ttatcgaaac aaatggacta tgcagagcca tatttaccag gttttaccca
     2701 aatctctgcg atcaataacg atatcatcaa tccactgatt gaaaaattat tagcaggaga
     2761 agatcctgag acactcatga acgatgcaga tgatgccatc aatcaattac tgcaaaaata
     2821 gtacagaact ttatacagga tagactgttg gcaagataca gatatctgaa tgatcgacct
     2881 agtatttgca gataggtgtt attgcaaaaa tcacagcgat tgttcggata tcttattcag
     2941 attcgtttaa accaagattg tatattttat caaaatgaaa gatgaggtgg actatatgaa
     3001 cggattcacc aaatattgga atcgtcctga tcgtgctgcg tatttgtttt taactccatc
     3061 ttttttagtt ttattattat ttactgtcat accgctttta ggaacatttg ctattagttt
     3121 tacagatttg aatattttct ttacatcaag aaattttgta agttttgaaa atttcacacg
     3181 tatatttagt gatgaacgag cagttaattc gttgattcat tctttatatt tcaccgtact
     3241 cgaaacgcca acacaaatta tcgttggttt attagcagca gtagcattat ctaaaaatac
     3301 cagattcaat cgtttttgtc gttcgacatt ttacattcca gttatttgtt cattgacgtc
     3361 aattgcgatt attttttcta tgttgcttga tccgaatgta ggggcgattc cttacttgtt
     3421 ttcacaagtc ggcttgccga tccctcaatt ttttcgcgat ccaacccttg ctatgccgac
     3481 ggtagccatc atgactgttt ggaagaattt tggtgttact atgacgattc tcttgactgc
     3541 tattcaggga atctctccct ctttgtatga atctgctcaa atggatggtg caacaaatcg
     3601 acagcagttt tttaacatta cgctacctca gatcgttccc tccttaggtt tttgtatttt
     3661 aacgaatctg attggttcga tgcaagtgtt tgaccaagta tatgttgcaa ccgctggtgg
     3721 cccacaattt aagacagaaa cagctgttca atatatttac agtcgaggat ttacagcacc
     3781 ttatgaattg ggctatgcat cagcactgtc ttcggttttg tttatcattg ttgcaactat
     3841 agctatctcc actaatgtgt acatgtcacg gaaagaaaaa caaatgaaat aggagggaaa
     3901 cctgtatggc accagaaagc tcgattacag ttactgtgaa tagtcgaaaa gcagttagtg
     3961 gtaaaaaaat cattcgaacg attagtatgc tagttatatt gttggttatt gcgtttcctt
     4021 ttttgtggct gattatttct tcttttaaac acgaaaaaga cattatctca tttccgccta
     4081 gaatatttgc agatagctat accctagata actatattaa ggtttggaca acgatccctt
     4141 tattagatta catcaaaaat acagtgattt ttgctggagg gacagtgatc acttctgtat
     4201 tctttgattc tcttgcgggg tatgcatttg ctcgtatgcg tttcaaagga aaatcagttt
     4261 tgttctattt cgttttgctg acaatgatga tcccattcca agtcttcatg attcctctat
     4321 ttattgaagt caatttgctt ggcatgcttg atacttatgc aggtttgatc attcctcgta
     4381 tgacaactgc atttggtatc ttcatgatgc gttccttttt tattacttta ccagattctt
     4441 tggaagaggc agcacgcatt gatggattaa gtgagtttaa tatttttttg aaaatcatgt
     4501 tgccattgag caaacctact cttttatcat tagggatttt cacgctgatg aatagttgga
     4561 atgacttgct ttatccattg atattgacaa gtagttcaaa aatgagaact ttaccagcag
     4621 ggttagcttt atttacaggg cagaacattt ctttttatgg acctgtaatg gctggaacgg
     4681 ttatttcaat gctgccatta ctagtggttt atatttttgc acagaaatat tttgttcaag
     4741 gaacagctat gtctggaatg aaagagtgag gaagatgaaa atagttgaga ctaaacttta
     4801 tggacagttg attgtggttt ctgattacat cttattaggt attctctggg taatcgtgtg
     4861 tttgcctgtt attactatcg tgcctgctac atgtgctgtt ttttatgtaa tggcgcaatg
     4921 gaaaggtgag ggatcaggaa atatcattta ttttttcttt caaggaataa agcaacattt
     4981 tggtaagaac ttgttcctta gtctgttaac gatcattatt tattttcttg ttaatcaatt
     5041 attagaagaa cagacaatcg tactttcaat tagtggatat gtagttttac ttatttattc
     5101 catgtttctt ttatcctgga tatatgtgat ttctgaaaac aaagatatca catttttaca
     5161 aatttttgaa caaagtgcat ggaaagtatt ttttgatttt ttgagaaata ttctttgttg
     5221 cgtattgtta ttaggatttg tactgctcat atttcttttc cctcctttta tttttatctt
     5281 tgctggtggc gtatggaaac ttgtatctat tatcttgact ataggaaatg aaaggtaggg
     5341 aaagctgttg gacgaagaag tgaaagtaaa aatcagagag gattttacgg taggagagat
     5401 cagttctcgg ctgtttggct catttgttga gcatatggga agtgtcgtct atacagggat
     5461 ttatgaaccg ggccatgaaa cagctgatgg tgacgggttt agacaagatg tcctctctta
     5521 tgtaaaggaa ttagaattag gtgtcattcg ctatcctgga ggaaacttta catctggtta
     5581 tgattgggtg gatactgtag gtaggaaaga acaacgacca aaaaaaattg atttagcatg
     5641 gcgaggtata gaaccaaatt cttttggact ccacgaattt tttaaatgga tcagtcatgt
     5701 acaagcgaaa ccgatattca cggtgaattt aggaacaaaa ggaattgatg aagcaaaaaa
     5761 tttagtagaa tattgtaatt ttccaaaagg aaccttctgg agtgatttaa gaataaaaaa
     5821 tgggcaaaaa aagccttttg gcgttaaatt atggtgttta gggaatgagc tagacggtcc
     5881 atggcaagtg ggggctaaga gtgcttatga atatggtagg ttagcaaatg aagcctcaaa
     5941 agtcatgaag ttagtggatg attcaattga aacaatctta gttggaagct ctacgccacg
     6001 tttaccatcg taccctgaat gggatcgtat cgcattagaa gaagcttatg agaatgtaga
     6061 ttatttagct ttgcataact atattgataa gtatgatgaa gaagacttga ctaaacctcc
     6121 taaaaatgag cgtgatgata cagcgacata ccttgcaaaa gcttatcgat tcgatcgtca
     6181 aattgaagaa atcgttgcta cttgtgatta tgtaaaagga tatttgagaa gtgagaaaac
     6241 aatgtatcta gcgtttgatg aatggaatgt acatgcccaa ccggaaaaaa gacataggga
     6301 ttttgaagtc ggtagtccca tcgattggtg ttactttact atggaagata cattattatt
     6361 tggttcatta gggctggcta ttttacgaca cgcagatcgc gtaaagatta gttgtcaatc
     6421 attgttagta aacacaatac ctttaatact gacagatgct aaaggacaag catggaggaa
     6481 tccaacttat tatgtgatgc aacacttatc gaaatatgct aaaggtcaag tattgaaaca
     6541 acaaatcatt tgccctgttt atgattgtga aaagataaaa aatgttcctt atgtggacag
     6601 tgtgatcgta gataacggca atcagctgac tattttcttg atcaatcgca caaatgaaca
     6661 acaaaagata acgattgaac atgatttcaa tttgctagat caaggagagc actacacatt
     6721 aaccagtagc gatctgttgg acaaaaatac acgtgaagaa ccagaaaaaa taaaacctga
     6781 aataaactat ttttcaatag aaaaacatga cgaattaaat gtagggatag aaaaatactc
     6841 ttggaatgta attagaataa agaaaatgtg aggaaatttg ttatgatgaa gacactttat
     6901 aataatccta tcgttcggca acgggcagat ccttgggtat ataaacatac agatggttat
     6961 tattatttta caggatcggt tccaggctac caagtgattg aattaagaag ggcaaagtca
     7021 ctgaatgaat tagaacatgc tgcaagcctg attgtatggc aggctcatga agaaggaccg
     7081 atgagtgaat tgatctgggc acctgaagtc cattatatca atggaaaatg gtatatttac
     7141 tttgctgcaa gcaatcataa aactatacgt gatgaatctc atcatcaccg catgttcgta
     7201 cttgaaaatc agcatataga tccaatgaat acagattgga tagaaaaggg acaaataaaa
     7261 acgaaaaggg aaactttcag tttagacgga acaactttct cttctcaagg aaagctatat
     7321 tatgtgtggg cccaacaaga tcctgaaatt actgggaatt ctaatcttta tctatccgaa
     7381 atggataatc cttggacgtt aaaggggaag cagatcctcc tttctatacc tgagtatgaa
     7441 tgggaaaaac ggggtttttc agtaaatgaa ggtccagcag tccttactcg caatgggaaa
     7501 atattcatta cgtattctgg tagtgcaaca gatgaaaact atgccatggg cctgctgtgg
     7561 gctgatgagc acaagaatct tttattaaaa gaatcatggc ataaattatc ggagccagta
     7621 ttcgtttctt ctgaaaaaaa taagcaattt ggtcctggtc acaactcgtt tacggtttca
     7681 gaagacgggt gctttgatat tttgatctat catgctcgac cagagaagaa ccaaaaaggg
     7741 gatccgttag acaatcctaa tcggcacgcg aacgcgcaat gctttacgtg gaatgagcaa
     7801 ggatttccag aatttggtga acctgttccg tatgccttat gagcacaaga aaccttgatt
     7861 tttgcttgaa taaaccttgc agactcgtta tgatatatgt acgtacaaat aaaaaggggc
     7921 aaggcaaatg gcaaaatatc aagatattgc agaagagata aaacaaaaga tccttcgtga
     7981 ggaatacgaa gtaaatacaa ccattccctc agaattcaag ctgcaagaaa tatatgacgt
     8041 cagcagacat acgattcggc aagcaatcgc tgtgttggta aatgagggct atctgcgtaa
     8101 agaaaaaggg tcgggcacat atgtagatga cagctataag aaagaaaaaa caccattgaa
     8161 cagaacaaaa acgatcggtg tcatcaccac atatctctct gattatattt ttccgtcgat
     8221 tatccgagga atcgaaaatg aattacgaga atctgggtat tcattgcttt tagccagtac
     8281 gaataacgat cacgaattgg agaaagagtg tttgaagaaa atgattgctt atggggtaga
     8341 tggtttgatc gttgagccta cgaaaagtaa tcagtataac ccaaacttag cactttatgt
     8401 tgctttgaga gaacaaggca ttccgatggt gatgatcaat gctgcttatg aagagttaaa
     8461 tgcctcttat atttgtttag atgatgtgaa agtcggtttt atggcagcag aatatttgat
     8521 aaaaaaagga catcagcgtc tgttgttcat cacaaaaatc gacgatcttc aaggaaaata
     8581 caggatgaaa ggttttatca gcgcgtgtga acagtataat gtgcgttttt tgacggaaga
     8641 tatcattact ttcaccacgg aaacacgaga aacaatcgct gaaaaaacag tacaacggtt
     8701 gaaagaagaa caagctttag atggcatcat ttgctataac gatcaaatag ctagtttaat
     8761 gctagataag ttgacagaaa atggctatca tattccagat gacctatcga tcgtaggaac
     8821 agacgattct tctttaagcc atttagggaa taaaaaattg acaacattga cgcatccaaa
     8881 agaaaagttg ggacaagatg ccgcacggtg gatcgtcaca acgattgaaa aaggacaggc
     8941 acagccaaat attctctatg aacctgtact agttgaacgg gaaacagtga aagaaaacga
     9001 aaaataagag acgggttttt accgcctctt attgctccta cggttactcg tcgcaatgtt
     9061 tgtgagagct tttgtcatcg ctcaaggctg aacgtttctt tcaccacctc tccactaaac
     9121 ggtgttctcc taccaac
//
LOCUS       DACENJ010000104         9000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.107, whole genome shotgun sequence.
ACCESSION   DACENJ010000104 DACENJ010000000
VERSION     DACENJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.107"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            166..603
                     /gene="zapA"
                     /locus_tag="IW748_001728"
     CDS             166..603
                     /gene="zapA"
                     /locus_tag="IW748_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1104638.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            661..1209
                     /locus_tag="IW748_001729"
     CDS             661..1209
                     /locus_tag="IW748_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1104639.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            1324..3684
                     /locus_tag="IW748_001730"
     CDS             1324..3684
                     /locus_tag="IW748_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1104640.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            3771..4085
                     /gene="trxA"
                     /locus_tag="IW748_001731"
     CDS             3771..4085
                     /gene="trxA"
                     /locus_tag="IW748_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1104641.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            4267..6045
                     /gene="uvrC"
                     /locus_tag="IW748_001732"
     CDS             4267..6045
                     /gene="uvrC"
                     /locus_tag="IW748_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1104642.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            6190..7089
                     /locus_tag="IW748_001733"
     CDS             6190..7089
                     /locus_tag="IW748_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104643.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            7082..8344
                     /locus_tag="IW748_001734"
     CDS             7082..8344
                     /locus_tag="IW748_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1104644.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            8505..>9000
                     /locus_tag="IW748_001735"
     CDS             8505..>9000
                     /locus_tag="IW748_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HAQ1104645.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKE"
BASE COUNT         3087 a         1483 c         1941 g         2489 t
ORIGIN      
        1 caactcacga cttttcgttt tctaagagat tgcgagacat agcatttttt taaagtttat
       61 catataatag ttaagtatgt gaaaaatatt tgtggagaac ggtgaaagaa ccaacttcat
      121 ggtacactaa ataaaacttt catcaaatag gaggaaatga ttcgtatggc gcacgaaaaa
      181 acacgctaca aagcagtgat tgccaaccaa acatatacta ttatcggacg ggaaaccaag
      241 catcatatgg atatcgtaac gaagctgatc aatgaacagt tagcagaatt gaaacaatta
      301 tcaccacaaa tggataatga acaagcagca atcttgatgg ctgtcaatgc gctttcggat
      361 caattgaaga aacaagaaag aatcttggag ctggaagaag aaacagcaga attaaagaaa
      421 aaaatgatca aatttacaga gcttgaaaat cgtgtgaaac gcattgaagc aatcgagaac
      481 gaagctcggg aagtattgaa agaaaatggt caagcagatc ataccattaa aaatcatgtg
      541 gaagcacaac agattttaaa cgagaaacga aaaggacaaa ttaaacaaaa atcttctaat
      601 taagatcctt ttatcaaaat aagaaaaatt taaaaaagtt agaagagaaa ggaacgtagc
      661 atgctgagtt tgctgatttt atttttattg ctaattgcgt ttttttctgg agcacgtcgc
      721 gggtttgctt tgcaaatcat attcgcgatt ggatatgtat tgtcatttat tgctgcccag
      781 catttttaca aaccattagc aagtcatttg gaattataca ttccataccc agctgtcacc
      841 ccaacatcaa agttagcatt ttttgatcag gtatttgctt ttcacctaga tgaagctttt
      901 tatgcaggaa ctgcttttct gattattttg ctaattggct ggctgcttac ccgatttgtg
      961 ggggtttttg tccatggatt gacgtatgtg ccgatcttga gacaggtaga ctggattgca
     1021 ggaggcatcc tgtcactaat catggcttac gttactatat ttttgatttt gcagttactt
     1081 gcctttgttc cattagatgt ggtccaaaat caattcggcg agcatagttt cgctcgtttt
     1141 atcgttgaga gaacgccatt tcttaccaac aagatgtatg agctttgggt aacgaatatt
     1201 ttgaattaag gaaaatagtg ctggaaaata tttcgttttt tgtacagagt agtttattta
     1261 agaaggctgc gtagcgtatg tccggcacag ctcttttttc atagtgaaag ataaggtgat
     1321 aaaatgaata accgaatcct tgaaacattg gaatttgaaa aagtcaaaca gatggtcaga
     1381 caatttgtag tgactgcaca aggaaaagaa gaactggcag aattggtacc agtgagtgag
     1441 aaacaaacga tcacaaactg gcttcaagaa actgaggatg gattgaaagt gcaacgtctg
     1501 cgaggcggga tccctatacc taaacttgaa aatatccgtc cccatatgaa acggatcgag
     1561 atcggagcag acttgaatgg agtggagctt gcacaagttt ccagagtatt gtctacgaca
     1621 agcgaattga atcgtttcat tgatgatctt tctgacagtg aaatcgaatt tgcacgtttg
     1681 tatatgtggg cagatcagtt agtcacgatc cctgttttga gccgccgttt aaaggaagca
     1741 atcgatgaag acggacgcgt gactgatgac gcttctcctg agttaaagag cataagacag
     1801 aacattcggc gaagtgaaca agctgttcgt gaacagttag atgggatcgt ccgtgggaaa
     1861 aatgcaaagt atttaagtga tgcgatcatc accatgcgaa atgaccgcta cgttatccca
     1921 gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc acgatcaaag tgcttcagga
     1981 cagactttat ttattgaacc aaaacaagtg gtggatttga ataatcgttt gcgtcaatac
     2041 caaatcgctg aacgaaatga gattcaacgt attttaagtg aactttctgc tgaacttgtt
     2101 ccccatcgtc aagaaatcat ccacaatgct tatgtgattg gtaaaatgga tctgatgaat
     2161 gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc cgggaatcag tgaagacaat
     2221 catgtcgtct tgaaacaagc aagacatcca ctgatcgatc aggaaaaagt agtttctaat
     2281 gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta caggacctaa cacaggtggg
     2341 aaaacgatca cgttgaagac tttaggtctg ttacaactga tggggcaagc aggattaccg
     2401 attcctgcag gtgaagaaag tcaaatcggt atttttgagg aagtatttgc ggatatagga
     2461 gacgaacagt cgattgagca aagtctatcg accttttctt ctcatatgac gaacactgta
     2521 gatattttat caaaagtcaa tgaaaaaagt ctggtattat ttgacgagct cggtgcagga
     2581 acagatccgc aagaaggtgc tgctctagca atcgcaatct tagatgatct tggtaaaaag
     2641 tctgcttatg tgatggcaac aacgcattat cctgaattga aagtttatgg atacaatcgg
     2701 gcaaatacga ttaatgccag catggagttc gatgtggata cactaagtcc aacgtatcgt
     2761 ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa tctctagccg attaggtctt
     2821 gatacagaag ttattgatga agcgaagcag ttgatgaatg atgagagcca agatttaaac
     2881 gaaatgatca cagatttaga gaatcgccgg aaaatggcag agaccgaata tctggaaatg
     2941 cgtcattttg tttctgaggc acaagaactt cacgatgatt tgaaagaagc ttacagctat
     3001 ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga aaaaagcaaa cgaagtcgtg
     3061 tcagaggcgg aagaaaaagc agaaaaaatc attgctgata ttcgtaaaat gcaacagcag
     3121 attgggcaag gaaatgtgaa agagcatcag ttgatcgatg caaagaccca attagcaaat
     3181 ctacatcaag aagaaacttt gaagaaaaat aaagtattga agaaggcgaa agaacaaaaa
     3241 acgttgaaac cgggagacga agtgttggtt acgacttacg gccaaagagg gacgctttta
     3301 cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat taaaaatgaa tgtgtccgaa
     3361 gacgagctga cgcctgttgc tcctcaaaaa gagccgacac aacgagttat ccatgctgta
     3421 cgatctgaat ctagtagtca cgtgccgaat caattggacc ttcgaggaaa acgatacgaa
     3481 gaagcgctaa gtgaggtaga tcaatatttg gattctgcca ttctagctgg ttatcctcaa
     3541 gtgactattg tccacggaaa aggaacaggc gcacttcgca agggaattac tgattatctt
     3601 aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg caaaccaagg cggaaatggt
     3661 gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa gcagatggtt cgctatccaa
     3721 gtatagaaat gctatactaa tcatagattt taaaaatgga ggtctttgat atgtcacaag
     3781 taattacaga taagacattt aatgaagaaa cagataaagg acttgtattg attgattttt
     3841 gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat tttggatcag ttggaacaag
     3901 aatatgatga agaagaattc agaattgcta aaatggatgt ggatgaaaat ccagaaactc
     3961 ctcaacaatt cggtatcatg agcattccaa cattaatgtt gaaaaaagat ggtcaagtag
     4021 tagaaaaagc tgttggtgtt cattcaaaag aacaactacg tcaaatgatc gatcaatatt
     4081 tataaaaact tacagcaaaa ataagctgag ggtgggactg aatccagttc cgtcctcggt
     4141 ttttttgcgg ttgagttaag gttattcaaa ttaatatttt ttgagtataa ttacgttgtc
     4201 aggctggatt gtttttttga cagacatgct ataataactt taattgaata tgaaggggag
     4261 taccagatga acgaacgaat caaaaacaaa ttagctttac ttcctgatca gcccggctgt
     4321 tacttgatga aagataaaaa tggaacgatc atttatgtcg gcaaagccaa aatattaaaa
     4381 aatcgtgtcc gctcatattt tactggcagc cataatacga aaacggaacg gcttgttagt
     4441 gaaatcgaag actttgaata catcgttaca gaatcgaata tcgaagcatt gctcttagag
     4501 atcaatctga taaagaaaaa tgatcctaaa tacaacatta tgttaaagga tgataaaacc
     4561 tatcctttcc ttaagataac gaatgaaaaa tatccccgtt tggtgattac tcgtaaagta
     4621 ctaaaagaca aagcactgta ttttggacca tatcccgatg tcggtgcggc gaatgagaca
     4681 aagaagatcc tcgatcgatt atttcctctg agaaaatgca aacctagtca gacgaaggag
     4741 ccttgtcttt actatcattt gggacaatgt ctgtgtcctt attatttcga cgtagatccg
     4801 aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt taaacggagg acacgaaaaa
     4861 atcgagtcag agatccatca gaaaatggaa aaagctgctg aaaacatgga gtttgaaaaa
     4921 gctgcagaat accgagacca gatccgtgcc attgagacaa tcatgactcg ccaaaaaatg
     4981 acaaatactg atttactgga tcgagatgta tttggttatg cgattgacaa aggatggatg
     5041 tgtgtccaag tgtttttcgt aagacaagga aagctaattg aacgagatgt ttcaatgttt
     5101 cctttctata acgaagcaga agaagatttc ttgacttacc tcggtcagtt ttatcaggaa
     5161 aatgagcatt tcattcctaa agaagtcttg attcctgata cgatcgacaa gcccagtgta
     5221 gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag gagaaaagaa aaaattagtt
     5281 gaattggcga ataagaatgc ctcagttgct ttgaatgaac ggtttgatct gattgccaga
     5341 aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa acgcgatgaa tatcccgacc
     5401 ccaatacgta tcgagtcttt tgataactct aatatcatgg ggacgaatcc tgtggctgcg
     5461 atggtcgtat tcattgatgg aaaaccggca agaaaagaat atcggaaata taaaataaaa
     5521 acagtcaacg gaccggatga ttatgcatca atgagagaag tcatctatcg cagatattca
     5581 cgagtgattc gcgaagggtt gcctttacct gatttgatct tgatcgatgg aggaaaagga
     5641 caagtagatg tggcaaaaga tgtcttagaa aatcaattag gaatcgatat tcctattgct
     5701 ggcatggcaa aaaacgataa acataaaaca aatgagttgc tattcggtat agatttggct
     5761 gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac tccagcgaat ccaagatgaa
     5821 gtccatcgat tcgcaatcac tttccatcgg cagctacgca gtaaaaacag ttttgcttcc
     5881 cgattagatg aaattgaagg gttaggacca aaacgaaaaa aaatgctgct taaagagttc
     5941 aaatcgttga aaaatatcac agcagccacg acagaagaat tacaagaaat cggactgccg
     6001 aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg aatgaggaaa gggcacttta
     6061 caaagaaagt aaagtgtcct atcattttat gtacaacaaa aagagcgttt acgttttgtt
     6121 ggtaaataat aaatcgctta cgtctggtgt ctaaggatat atgtgatttc ttgaagaagg
     6181 agtgaagaaa tgttagaagt caaggatctt gttaaaactt ttgggaacta cacagctgtc
     6241 gatcatgtct ctttccaaat ccccgatgga aaaatcatgg ggctaattgg ccaaaacggg
     6301 gctgggaaga caacgacttt tcgtttgatc ttggattttt tgacacaaga ccaaggagaa
     6361 gtgttatgga atggccatca tcttggggag aaagattatg atgtcatcgg ttatcttccg
     6421 gaggaacggg gactttatcc gaaagtaacg attcaagacc aactgcttta ttttgcagaa
     6481 cttagaggga aatcaagaaa agagattgaa ccaaaaatcg atttttggat ggaaaaattt
     6541 caggtaaaag ggaaaaagac ggataaagta aaatctttgt ctaaaggaaa tcagcaaaaa
     6601 gtccagctga tcgccacgtt aatccatgaa cctaaattga tcattttgga tgagccattc
     6661 agcgggttag atccggtaaa tgctgaattg ctaaaagatg gaatattgga attgaaaaaa
     6721 catggttcat gtgtcatttt ttctagccac aatatggata atgtagaaaa aatctgcgat
     6781 catttgatca tgttgcgaaa cggaaaaatg gttttgaatg gaaaagtaca tgagattcgt
     6841 gaatcatttg gccgtacgaa gttattcttg gaatctggtt taagccaaca ggaagtagaa
     6901 gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca atacgttaga gattacctta
     6961 gaagatcctg aagctgggaa agaaatcttt actcgtgcga ctcagttcgg ctacattcct
     7021 atgtttaacc agcaaccgcc aactttggaa gagattttca aattgaaagc aggtgaaatc
     7081 gatgcgtaag ttttggatta ttaccaaaga tgtctattta aaaaatgtta agtcgatttc
     7141 tttcttgatc atgatattag tcccttttat catgctgggt atcatttatg tagcaggaaa
     7201 ctttgctcaa caaaatagtg atgtcaataa aatcggcgta ttggctgata atgagcaaat
     7261 cagtcagcaa atgagccaag tcaaaacaga tgattttgag ttcaaagcag ttgattctga
     7321 agaaaaagca caaaaagagc tttcggaaga aaaaatcgat gcgtatttag tagtctcaac
     7381 tgagaatgat aagataaatg ggatgcttta tagcgaaaat tcattaggac aaagtacaca
     7441 actactgata caacaacaac tgactggctt gcaatcgatg cttcgagcaa gtcaattagg
     7501 catttcacca gaagaagtag ctagcttgag ccaacaagca agtttgactc gacaaaaagt
     7561 caattttgat tcaaaaggaa aaatgacagt tggagaagat aactcgacag tccaatatgt
     7621 cgtaagttat gtagcaacga ttgtcttgtt cattattatt ttgacgtatg cgcagatcat
     7681 tgcacaagag atcgcttctg aaaaaggcac gcggatcatg gaagtgatcc tgtccagtac
     7741 gactgcacaa aagcattttt acggaaaact aacaggtgtt ttacttgttg cattaacgca
     7801 aatggcatta tacggtatta tttttggtat aggatacagt caattcaaag atatggaaat
     7861 ggtcaaaaat atattagacg gtatctcatt ggataacttg attggaccat tcttgtggta
     7921 ttctcttcta ttcatgatct ttggcatttt gattttttct gttttagcag ctttatgtgg
     7981 ctctttggtc aacaaagcag aagatacagc aaaagcaatt ttgccagtga cttatctatc
     8041 tctaggcgga tatatgcttg gattgatact aggagcttca gatccaaaca atatcgttat
     8101 tcggatcact tcgtatattc cattcttatc ttcatatatt atgccggttc gcttagcaaa
     8161 tgagacagtc ggtatgggtg gagcattgat ttccttgttg gtacttgtag ccgtaacatt
     8221 cttattgatg atcttgtctg caaacatgta caaatcgaat gtactcgtgt atagcgaagg
     8281 cggattatgg agttcattga aacaatcgat ttcaattatg agaaatgaac gaaaaaaagc
     8341 ataaaatata gaaaaatata gaaggaagct ggacatttat tgttcccagc ttcttttttg
     8401 tatagaccta tttctatttt tttattatat tggatatata ttcattgaaa atagagcata
     8461 acaactttat aatgttgagt ataccaattt ttggaggcgt tttaatggac cgctatttag
     8521 atttactttc agagaagttc cctacaacgg aagatgtcat cacggaaatc atcaatctcg
     8581 aagcaatttt gcagcttcca aaaggaacag agttattttt gagcgatata catggcgaat
     8641 ttcctgcttt tgatcatatt ctgcgtatcg gttcaggaaa tcttaaagaa aagatcaaag
     8701 accttttctc tgaacaaatg acggaaacgg agatcagcca attcactatt tttacggctt
     8761 atccagaata tgtgttgacg actgattggt acaaaaaaca agataaatcc cagctgatcc
     8821 atcgtttgat cgatctttta cggtttacga cagtcaaata cactcgatca aaagttcgaa
     8881 aaggattgcc gaaagaatat agctatatca ttgaggaact cttgtatatc gatgatcgta
     8941 tcaacgggaa aaaagactat gtaaaaaaaa tcattgaaca acttgtcttg atgaaggaag
//
LOCUS       DACENJ010000105         8989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.108, whole genome shotgun sequence.
ACCESSION   DACENJ010000105 DACENJ010000000
VERSION     DACENJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.108"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(18..1247)
                     /gene="hflX"
                     /locus_tag="IW748_001736"
     CDS             complement(18..1247)
                     /gene="hflX"
                     /locus_tag="IW748_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1104646.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1249..2166)
                     /gene="miaA"
                     /locus_tag="IW748_001737"
     CDS             complement(1249..2166)
                     /gene="miaA"
                     /locus_tag="IW748_001737"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1104647.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2170..2907)
                     /locus_tag="IW748_001738"
     CDS             complement(2170..2907)
                     /locus_tag="IW748_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1104648.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAENPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2998..3525)
                     /locus_tag="IW748_001739"
     CDS             complement(2998..3525)
                     /locus_tag="IW748_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1104649.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3705..4298
                     /gene="clpP"
                     /locus_tag="IW748_001740"
     CDS             3705..4298
                     /gene="clpP"
                     /locus_tag="IW748_001740"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1104650.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4402..4887)
                     /locus_tag="IW748_001741"
     CDS             complement(4402..4887)
                     /locus_tag="IW748_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1104651.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5040..5237)
                     /locus_tag="IW748_001742"
     CDS             complement(5040..5237)
                     /locus_tag="IW748_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104652.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5332..7092)
                     /gene="efrB"
                     /locus_tag="IW748_001743"
     CDS             complement(5332..7092)
                     /gene="efrB"
                     /locus_tag="IW748_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1104653.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7089..8819)
                     /gene="efrA"
                     /locus_tag="IW748_001744"
     CDS             complement(7089..8819)
                     /gene="efrA"
                     /locus_tag="IW748_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1104654.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2537 a         1990 c         1502 g         2960 t
ORIGIN      
        1 gttaaaaaaa taaaatttta ttggtttttt cttaaccatt ttgaattggt ttttgcaaac
       61 cctttaactt catattcgtt tgtttcttcc atgtacgctt cgtccaaaac cattgtttgt
      121 cttctcaact cgcttaattt ctgtccttcg ttgcttggca cagaaagtgt ataaggggtc
      181 attagttcca tcagctgacg tttgattgct tcgatcaacg ccatcttacc ttctttcgtc
      241 tgagtagaaa tcaatacatt tgggaatagc gtaggcgtga acatcgctgg gtcgatctgg
      301 tctgccttat tgtaaacagt cagtatcggc atttctttca tattcagttc atccatcaat
      361 tttaagacag tctgctcctg taaaatacga tctggagaac tagcatcgac tacatgtagc
      421 aaaagatcca tattttgact ttcttctaat gtagaatgga aagcatcgat cagttgcgta
      481 ggcaggtctt ggatgaaccc gacggtatcc gttacagtaa tctcaaatcc ttcagcaaaa
      541 cgccatctct tagtcaatgg atctaacgtt gcaaacaatt gatctttcga ataagtatcc
      601 gcttgtgtca atagattcaa gatagttgac ttcccggcat tcgtgtagcc aataagtcca
      661 atctgaaaaa tctcgctatt ttttcttttt tggcgattgc gctctcgatg agcttctact
      721 tctttcaatt ctttcttaac agcaaaaatt ttattccgta tatgtcggcg gtctgtttct
      781 aactttgttt cacctggccc tcttgttccg atcccaccgc caagacgaga taatgttttc
      841 ccttgcccta ctaaacgagg cagcaggtat tctaactgag caagttcaac ctgcagcttc
      901 ccttctttcg aacgagcacg catcgcaaaa atatctaaga taagctgcac gcgatcaatt
      961 accggaagac caacagcatc ggtaatcagt tggctctgtc tcggagttag ctcatgatta
     1021 aagatgatca aatccgcttc atgcgcatcc gcttgttgaa ctaattcttg taatttccct
     1081 ttaccgataa tcgtttgccg atccacttgt ggtctctttt gtgtaattgt gaatacgact
     1141 tctcctgatg cggtattcgt cagctgaact agctcttcca ttgactctga aaagtaacgc
     1201 tggtttgctt cggtttctac ccctacgatg atcacttttt cttccatttt attccgcctc
     1261 cagccatttt ttgattgttt gctcaatctg acttttttga tttggctcac ttataagatc
     1321 aaaccactgt gcatccaaac ggttacgaaa ccacgtcaac tgacgtttcg cgtatcttct
     1381 cgaatgtagt ttgacttgtt ctgttgcttc ttccaatgtt tctttccctt caaaataagg
     1441 gaaaaattct ttatatccaa taccttgact ggcctggact tctccaagct catacacctt
     1501 acgcgcttct gctactaaac cttctgctag catcaaatcg actctttgat tgattcgttc
     1561 ataaagaata ctacgcttcg tatctaatcc aatcatgagg taatcataca attgctttgg
     1621 ctcttcttta ggtgccgtga tactatgtcc tgtcgtctct aaaacttcca gagctcttat
     1681 cactttgcgt tgattgttcc agtggatttt ctcagcagct gccgggtctt tttctttcaa
     1741 ctgattccat aatgcttggg aaccaattgt ttcagctaac gtttcatact tttttctaat
     1801 ctcaggattc gcttcttctg tcgctcctaa ctgataatca tatagaaggg attggatata
     1861 gagacccgta ccaccagcta tgataggtag gtgccctcgg ttcgcaatct cggtaatcaa
     1921 ttgtcgacct gaatgctgaa aatcagcggc tgagtaacgt tgagtaggtt cgatcacatc
     1981 aatcaaatga tgcggaacat tattcatctc ttcttctttg atttttgctg taccgatatc
     2041 cagattttta tagatttgca atgaatcacc actgatgatc tctcctccaa atagttgtgc
     2101 gagttccaca cttagcgctg ttttaccgac tgctgtcgga ccgacgatca ctaatacttt
     2161 ttccatattt tacctacttt tcgttgattg tttccaccga agagctttct ccggaaaatc
     2221 agttattatt ccatctattt tgagttcttg acatttggcc atttcttctt catcgttaat
     2281 cgtccatgca cgaattgctt ttggaaaatc agctgcctgg actggatgtt cgaacaacca
     2341 agtatttttt ggatgtactc cttctatata agggttttct gctgctagtt ggatcttttt
     2401 atcagaagta ctcatcaata agtccagctg tatctccggt tccagtaatg cgattttttc
     2461 caaagaatcc aaatgaaagc tgcaatatgc atgttgaaaa ggccaatctc tactttttaa
     2521 tgtttcggct aatttttctt cgataccggg atattcatac tgatcagttt taatttcaat
     2581 cattagcatc cctcggtaat ttttttggag gagcagtcct aacacttctc tcaattcagg
     2641 aactttagct gctggaaatt ctttgctgaa tttgccccct gcattcaatt ttttgagttc
     2701 ttccaatgtt ttttctcgaa tcagcccttt accgttagtc gtccggtcca cttcttcgtc
     2761 gtgcataacg atcaaataac catctttgct tagatggaca tctagttcga taccatctgc
     2821 acctactcgt acagcttctg caaatgcagg aagcgtattt tctgggtggg tgccgctgct
     2881 tccgcgatgg gcatagattt gtaacatctt tcttcactcc gttcagttct attgtatcat
     2941 taagaaaaaa agaaagcatt accacgctct catgtgcaac ggcaatgctt caaaatatca
     3001 gctccaaaca tcatctttcg agaagaaacc aatcggctgg cgcgtcatag tcttcaactg
     3061 agcttttttt tcattgatca tttcagtcag actatccgta aaattctcta aaatcacgcg
     3121 tccgccttta taagcatatc caccaatctt cattgtttca tctggagata aaatcacttt
     3181 tcgatgggag atattcttaa atagctcgtc taaaatataa tttggcagat aaaaactatt
     3241 ttgcgctgaa aatgattgga gatacgcaaa atcgtcaatc gtgatataac aatcatcaaa
     3301 tgcatcaaag ctgacatatt cttccatcag cgttgtccgt ttttgcaaga tctcgttgat
     3361 ctttttattt aattgattca gcgtctcgat ttctttgact ttcaattcca attcacgtct
     3421 acgctgttct ttattttcca agtgacgcat ttgcagtatc atgccgatat agacaactac
     3481 agcacctgtc agcactccga taaggccagt aataccaact atcaatctgt tgttcctcct
     3541 tgttttgttt ctctgcttat cataccataa agcgggttta tgcgactaat ttgcctaagt
     3601 tttttatttg accttttttg accaaaaatg tatactacat tttaggtatt agaaactaaa
     3661 ttatggtaca ctatttctaa acgaaatcaa ggaggaactc tattatgaat ttaattccta
     3721 cagtcattga acaatcttca cgaggtgaaa gagcttacga tatctactca cgtttattga
     3781 aagaccgtat catcatgtta agcggccaag taacagatga tcttgctaat tcgatcatcg
     3841 cccaattgct tttcttagac gctcaagatt ctgaaaaaga tatctatctt tatatcaatt
     3901 ctcctggtgg ttcagttaca gcaggtatgg ctatttacga cacaatgaac tttgtcaaag
     3961 cagatgtcca aacaattgtt atgggtatgg ctgcttcaat gggaagcttc ttgctgacag
     4021 ctggtacaaa aggtaaacga tttgctttac caaatgctga gatcatgatc catcaacctc
     4081 ttggcggcgc tcaagggcaa gcaactgaaa tcgaaattgc ggcacgccat attttgcaaa
     4141 cacgcgaacg cttaaacaaa attttagctg aacgcactgg acaaccgctt gaagtgattg
     4201 aaaaagatac agatcgtgac aactatatga cagctgaaca agcaaaagca tatggattga
     4261 tcgatgaagt catggaaaac agttcttcat taaattaatc tgtttcaaag gaagcttttt
     4321 taagctctct tatctaaacc aaataaaaaa ggatggtcaa ctcaaactga ccatcctttt
     4381 ttattttatg gatgctactg cctatctttg ctccttgaat tttccttgcg catgcatccg
     4441 acctatctcg acataatgct ggatgttttc tttgtttttt tgtatttctt cttttgtcaa
     4501 aggacggatc actttagctg ggcgaccaaa agccaagaca ttaggaggaa taacttttcc
     4561 ttctgttact aatgatccag caccaatcaa actattctca ccaataaccg catgattcaa
     4621 tatcgtggtc cccatcccga tcagcgcccc tttttcgatt gtacagccat gtagcatgca
     4681 ctgatgtcct accgtcacat tctccgcaat atctacaggt gcatcatgat cgacatggat
     4741 gattgtacca tcttggatat tcgttctttg tcctattttt atccaattac tgtccccgcg
     4801 taaaacagct tgataccaga tcgtcacatc ttctgacaat gtcacatcac caatcactgt
     4861 ggcatttgat gctataaacc gttccatatt tatttttctc ctttcatttt ttgtagaaac
     4921 taatcctatt gaataaaggt atcatagcat atatttattt cataagaaaa aaaacaggta
     4981 aaacctgctc aggccagatg tctatttaca gtatctggta actgacgttt tacctgtttt
     5041 tattgtacat ctatattttc ttctattact ttttttaaaa ttaattcgta cacttctatt
     5101 tgtgctgcca agcgttcgat ttgaaactca ttttcaaaaa aatcagctga atcaaaaggc
     5161 ttcgtctgac agacgtgcgt taaacgatca acttctcttt tggcttcagt aattttttca
     5221 tttaaaaact gttccatctc cgtgtcctca cttttcttga taactttact ttaccattat
     5281 caagaataga gaacaaaaaa taagttttaa actgttttat tcttatatga cttattctgc
     5341 ttcctcagca aattggctgt tatacagttt ttcataaaat ccaccttgtg ccaataaact
     5401 ctcatgcgtt cctttttcaa tgatctgacc ttgatccatc actagaatca gatctgcatc
     5461 acggattgtc gataaacggt gagcgatgac gaaacttgtc cgtccctcca tcacgcgatc
     5521 cattgctttt tgaatcaacg cttctaaacg ggtgtctact gaacttgttg cttcatctaa
     5581 aatcaagatt ttaggatcgg agaccactgc tcgggcaatc gtcagcaact gtttttgtcc
     5641 taaagacaca ttgtctccct cagaattgat ggccatatca tatccatctg gcatcgttcg
     5701 gataaaatga tctacgtttg ctgttttcgc agcatcaact acttcataat ctgtcgcatc
     5761 aagctttccg aagcggatat tatctgcaat cgttccttga taaagccatg catcttgcaa
     5821 gaccatacca aatagcgaac gcacatcact gcgactcatt gatttggtgt cgattccatc
     5881 aatccttatt gctcctttgt ctacgtcata aaaacgcatc aatagattga tcaatgtcgt
     5941 cttgccggca ccggtcggac cgaccacagc tacggtttga ccagctttca cttcaaaatt
     6001 caaatcttgg attaatggtt tcttcggatc atatgcaaaa tcgacatgat cgaatgtcac
     6061 atctcctaaa atcgtttctg gcagtgaagc atctactttg ttttcttttt cttctggctc
     6121 atctaaaatc tcgaatacac gtttcgttgc cgctgatgca ctttgcaaaa ctgaagacaa
     6181 ttgtgtcacg ttccccattg gctgactgat ttgccaaata tattggatga atgcttgtag
     6241 ttgaccgaca acgatcgccc cagtaataac ataaaagctt cctagaacag ccataccaat
     6301 ataagtagca taggcagtta attgaactag aggcatcatc aagccagaaa tgaatgctgc
     6361 acgaaaccca aagtatttca agctgtgatt aacttttttg aatccttcca atgtttcttt
     6421 ttcgcgtcca tatactttca atacactaaa acctgtcata ttttcttgga cgtagccgtt
     6481 tagctcacca agagaattct gcatgctttg aaaatatttc tgcgaagcat tgatcactcg
     6541 ttttgagatc cagatcgatg ttggaatcat gatcatagag atcagcgcca tcatcggctg
     6601 gatataaaac atcattgctg cagccaacgt aattcccaat atcgcattta cgataccaat
     6661 aaaagcttgt tgtaaagcac cgctgactgc atctacatca ttcgtcacac gagacaaaat
     6721 atttccttgc tggtttttat cgaaatatga aaccggaaga cgattgattt tttcttctat
     6781 gtctcttctc aaatcatgca tggcactttg aacgacattt gtcatcaaaa caccagaaag
     6841 taattgcgaa atacaatacc ctattccaac aactgtgatc caaatgagac atttgaatat
     6901 ataagaaaaa ttgattgatt cgccattggc aatattacgg ctgatttctg tagtaggaag
     6961 tcctgtaaca tatggcaaag ccacgttgaa agccactgtc agaactgtaa ataggatcac
     7021 taaaagaaaa gtctcacgat acggtctgat atatcgagct aaacgtttta aggaagaaaa
     7081 ggtttttttc atgctaattc ctcctttgat aattgagaag cggcaatatc ataataaatc
     7141 gggcattttt ccaacaactc ccgatgcgtc cccataccga cgacttctcc ttcatttaat
     7201 acgatgatct tatctgcatg catgatcgtt cccacacgtt gtgctacgat caatacagta
     7261 gattctgtcg tttctttttt cagacgagca cgtaacttcg catccgtttg ataatctaat
     7321 gcagaaaagc tgtcatcaaa aatatagatt tccggacggc gtatgatggc tctggctatt
     7381 gccaatcgtt gtttttgtcc acctgaaaaa ttggaaccac cttctgataa atgttcatta
     7441 aatccatctg gttttcgaga aatgaattct gaagcttgag caatctcagc tgcacgttcc
     7501 atttcttctt gcgtagcatc ttctttcccg tatcgcaaat tatctgcaat cgttccagta
     7561 aatagcagcg ctttttgcgg aataaaccca atcttatctc gcaacttgct tagccgataa
     7621 tctctcacgt ctactccatc gatcaagatc tttccttcac tcacatcata aaagcgaggg
     7681 atcaattgga tcaacgtaga ttttccgcta ccggtacttc caataaaagc aacggtctct
     7741 cctggagagg ctgtaaaact gacatttcgt atcactggct cttgcgaatg gcctggataa
     7801 gcaaatgtta cattttgaaa ttcgatatac cctttagttt ttgtatcaga tataccattt
     7861 ggatcttcta caatcgaagg tttggcatct agggcttctt ggatacgtcc agctgaaaca
     7921 gctgctcttg gatacatcat gaacacacta gcaaataaca tgaatgagaa caaggcatgg
     7981 aatatatact cgataaacgc aatcaaatct cctacacgaa gctcgcctgc atcgatttgc
     8041 actgccccgc tccagatgat caataccatt acgatattaa ataagaaata aaaacccggt
     8101 tgtgcaacag ccatcaaacg gaataagctt tttgaactag aagcataatt ctcattgact
     8161 acagcaaaac gtttttcttc aaacttttca ttgacgaatg cacgaatcac acgcaatcct
     8221 gaaagatttt cccgcaagat actgttgatt ttatctaagt ttttttgttg ctttttagat
     8281 aacggttcag acaccttagc aatcgctacg actgccaata atagtaaagg taatgcacct
     8341 actacataca aactgagtgt cacacttgta cgcatgacca tgtacaaact gacaatgaac
     8401 atcattggtg ccataaaacc gatacgtaaa atattttgca ggaacaacat gatctgataa
     8461 gcatcgtttg ttgtacgagt gatcaatgaa gatacgccta gattttcgta ttcctgatgc
     8521 gaataacttt ggatcttttc gaacaaatca tctcgaatat ctgctacgat attagtcgta
     8581 atcctcgaac caaaataacc taacaagata ttcaaaataa taccgattac agtaataacg
     8641 atcataaata tcccttgttg cttcacataa tcaaaatcat cattgcggat tcctacatcg
     8701 atcatacgtg ctaaaatcgt aggaagtcct aattcaatca aaataaaacc aaatacgcat
     8761 atgaaattga aaaacaagaa tttcttataa cgcatggtat aatgccacat tagtttcact
     8821 ttctcatctc cttttaatct tttgttacat tttcattact aaaaatgata gcacaacgag
     8881 cctattaata gaacacttct ctcataaaaa agtcattatg attatagcgg gatgataaac
     8941 attgattttt tctttcaatc tagttgattt ctatacttat ttgctgttt
//
LOCUS       DACENJ010000106         8978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.109, whole genome shotgun sequence.
ACCESSION   DACENJ010000106 DACENJ010000000
VERSION     DACENJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.109"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            102..251
                     /locus_tag="IW748_001745"
     CDS             102..251
                     /locus_tag="IW748_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1104655.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            262..1776
                     /gene="dltA"
                     /locus_tag="IW748_001746"
     CDS             262..1776
                     /gene="dltA"
                     /locus_tag="IW748_001746"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1104656.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1773..2978
                     /gene="dltB"
                     /locus_tag="IW748_001747"
     CDS             1773..2978
                     /gene="dltB"
                     /locus_tag="IW748_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1104657.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3023..3256
                     /gene="dltC"
                     /locus_tag="IW748_001748"
     CDS             3023..3256
                     /gene="dltC"
                     /locus_tag="IW748_001748"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1104658.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3259..4527
                     /gene="dltD"
                     /locus_tag="IW748_001749"
     CDS             3259..4527
                     /gene="dltD"
                     /locus_tag="IW748_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1104659.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4668..5750
                     /locus_tag="IW748_001750"
     CDS             4668..5750
                     /locus_tag="IW748_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1104660.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5750..6553
                     /locus_tag="IW748_001751"
     CDS             5750..6553
                     /locus_tag="IW748_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1104661.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6623..7363
                     /locus_tag="IW748_001752"
     CDS             6623..7363
                     /locus_tag="IW748_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1104662.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7374..8378
                     /locus_tag="IW748_001753"
     CDS             7374..8378
                     /locus_tag="IW748_001753"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1104663.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8423..>8978)
                     /locus_tag="IW748_001754"
     CDS             complement(8423..>8978)
                     /locus_tag="IW748_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1104664.1"
                     /translation="VQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVT
                     NLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLY
                     RKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGT
                     LTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         2849 a         1496 c         1848 g         2785 t
ORIGIN      
        1 caaaactgtt agtaaaaaaa cggtttctgt aagctagaca catgtttctt tttgtcaaaa
       61 ttagttacac tttgtgtgag gttatatgag gagggaatcc aatgaacaaa tggcgttcta
      121 atccaaaatt gaattattgg ggaaaattca tcattcgaac aattttttat agtgcagtac
      181 tgctgttcct gatttatctg tatcactata aaaatattca aggaggaact tttatctata
      241 acgaatttta ggaggaagag tatggaaatc aaaacgatta ttgaagcaat cgatgattgg
      301 ggaataaatg aacctgatcg agttgcttat caggcggatg agacgcatac ttttggtgaa
      361 ttgaaacaag catcagacgc acttgcttat tatttagaaa aaaaggtaga aggcgatgga
      421 ccaattgtcg tttttggcaa tttggagttt gaaatgattg tctcatttct aggggttgtc
      481 aaatctggcc atgcctattt gccgattgaa gagcatacac ccaaggagcg tatcctatcg
      541 atctttcggg tggctaaacc atctatggtc atcagtatcg gggactggat agaagatatt
      601 ccttcggttc ctgtcataac aaaaaaagaa tttcaagaaa tcaagaagat accagtagga
      661 ttttatccca gaaatagcgt aaaaggtaca gaaaattttt atattatttt tacttctggt
      721 actactggag aaccaaaagg cgtacaaatc agtcatgata atctagtcag ctttgttcaa
      781 tggacgatgg aagactttgg gatcaagcct ggtatgcatt tcttagccca agcgccattt
      841 tcttttgatt tatcggtatt ttctatttat cctgcattag tttctggcgg aatgctgaag
      901 ccgttaaata aagcagttgt tcaagacttt cgtcaattat tcgcgacatt gccagccttg
      961 aagttgaatg tgtgggtttc tactccatca tttatggata tttgcttgat ggaacctaca
     1021 ttcaatggag aaaatgtgcc ggaattggac atgtttttat tttgcggaga agaattgacg
     1081 aaaaaaactg cagaaagtct acttgatcga ttcccaaatg cacggatata taatacttac
     1141 ggtccaacag aagcaactgt agctatttca agtattcaga ttgaccagca tgtactagat
     1201 agttatgacc gactaccgat tggctacgta aaaaaagaca cgaaagtttc tattgtccaa
     1261 gaaggtaaac ctgtttcaaa aggagaaacg ggtgaaatca ttattgcagg accaagcgta
     1321 tcaaaaggtt acctgaacaa tcccgaaaag acagctgctg cgttcttttg tcaaaatgac
     1381 ttagcaagtt accatacagg ggatgcaggt agactagatg aatatgggct tttattttat
     1441 gaaggacgta tggatcagca ggtcaaatta catggatacc ggattgaact cggggatatc
     1501 gaacattatc tgctccaaga caaccgaatc aaacaagctg tcgtcgtacc aaaataccaa
     1561 ggaacaaaag tacagcagct ggttgctttt gtcgtattag aaaacaaaac gaaacagccg
     1621 gattttcagt tgactaaatc tatcaaagaa caattactag aagttgtaat ggattatatg
     1681 attcctcaga agttccatta tgtcgatctg ctgcctcaga cagttaatgg aaaaatcgat
     1741 cgaaaaaaat taacagctga ggtgaatcct gaatgatgtt acctcatatg atcccttatg
     1801 cagagccgtt ttattttttt cttttagcca ttgcgcttgt accgatcgtt ctatctttat
     1861 tgatatggga aaaaagatta ccagtttatc aatcagcagt gacacttttc tttttatttg
     1921 tcagctttgg aggtaattat tggcaacagg gagcggcgct gattgggtat gttttgtggc
     1981 agacgatact tgtatggggc tattttgttt atcggcaaag gaaaaatcag acaggatggt
     2041 tttttgccgc cgttatttta gcgataattc cactgttttt aacgaaagtg actccttttt
     2101 ttgaacatgg aaaagcatcg ttacttggtt ttttaggaat ctcttattta acctttaaat
     2161 ctgtacaaat gatcatggaa ataagagatg gcatgatcaa aacatataag ccatttcgtt
     2221 atatccagtt tctgctgttc tttccaacaa tctcttctgg accaatcgat cgctatagga
     2281 gatttgaaaa agatcagctc catccgcctg ttcgtgataa atatgttgat cttttgggaa
     2341 aaggcgtcca aaatattttt gtcggttttt tatataaatt cattatcggt tattattttg
     2401 gtcaagtact gcttcctgta gtgggacgca ttgccttaca gcatggtggt atttcatggg
     2461 cattagtagg ttatatgtac gtgtacagta tgtatttgtt ttttgatttt gctggctata
     2521 gcttatttgc agtcggtaca agctacatga tgggatatca gacaccaatc aatttcaata
     2581 aacctttttt atcatggaat atcaaagaat tctggaatcg ttggcatatg acgctgtcct
     2641 tttggttccg tgattatgta tatatgcgat tgatgttctt catgatgaag aaaaaatttt
     2701 ttaaaagcaa gatcgtaatg tcaaatatcg gttattttgc tttgtttctt ctaatgggta
     2761 tatggcatgg attgacgtgg tactacatcg tatatggact gtatcatgct tgtctgatct
     2821 gtctgacaga tgcatggatc cgatttaaga agaagcataa agagcagttg ccttctaaca
     2881 aatttacgca tggcttagcc gtatttttga catttcaagc agtatgtttt agcttcctga
     2941 ttttttcagg atttttagat aaactttttt ttagctaacg aatagtgaca tattaattta
     3001 tataacaaag gagaaaagga aaatggaaga acaaatttta tcaatcttag aagaaataac
     3061 aggaacagat gaagtaagaa aaaatcgtga tattgatcta tttgatgaag ggttgcttga
     3121 ttctttagca tctgttcaat tattagtcga gctagacgga gagctggata tccaagtccc
     3181 tgtatctgaa ttcgagcgag aggattggag cacaccgaat aaaatcattc aacaagcaac
     3241 tgcattgaag ggttgagtat gaaaaagaaa ctttttgcta ttttcggccc ggttgtttta
     3301 gctgttggct tactgtttgt gttttttacc tcctcctatc aagtagatat ctctaatcca
     3361 gaaacgatta gacgtgcctc ttcttccatg tcagtgaacg tgttgaaagg agatgcgata
     3421 aaaaattatg cattatctga aaaacaatat attccttttt ttggctcaag tgaattaagc
     3481 agaatcagtc catttcatcc gtctgttctg gcagaaaaat accagcgaaa ctatcgtcca
     3541 tttttgctgg gagcacccgg gacacaatcg ttaagccaat atatgatgat gcgttctgca
     3601 ggagatgcta tgaaaaacaa gaaagtcgtc tttattattt ctcctcagtg gttcgttaaa
     3661 aatggtgtaa aaactgatta ctttaatacg tattattctg aacttcaaac atacgactgg
     3721 ttgttttcaa tgaagaaagt aacgcctgct gatcgctatt tagctagaag attattaact
     3781 ttctcaaaag taaaagagaa tgatacgttg acggcgattc tgcaaacgat caaaaaaggg
     3841 aagctacctt tacctgaatc tttgaatcag ctgcgcagcc aatggaacat gctgaagaga
     3901 gaggatgaag tattcagcaa tattgggttg aaagatcgac agcaaaaaat cgatcatgag
     3961 tcaaaacgat taccaaaaca ataccaagaa acagaattat ccattttagc aaatcaaatt
     4021 ggagaacgag aaacgacgaa taatccattc gggttgaaaa acgattttta tacgcatcgt
     4081 atccgagccc acgagccaga gctcaaacag tcacaaaaaa attgggatta tcgtttttcg
     4141 ccagaatttt ctgattttca gctggtcttg gatcaattag caaaaaacca taacgaagta
     4201 ttgttcatca tcccaccggt taacgagaaa tggagcgatt ataccggact atctcaagaa
     4261 atgttacaag gatttgccaa aaagatcaaa tttcaattaa atagccaagg attcaatcga
     4321 atagctgatt ttgtcaatca agcgggtacc aactatttta tggaagacac gatccatcta
     4381 ggctggaaag gctggttggc tgcagatcaa cagatcagac cgtttttaga agaaaatcat
     4441 attactgcta gtaagtatca tcttgacgat gcttttttta gcaaatcttg gcaacatcaa
     4501 ataccagata aactacaatt aaaataaaga aaacaggcga gtgttgctcg cttgttttct
     4561 ttatttttga atattctgaa taaaagataa aaataccaag tttgcctgaa caaaaatcat
     4621 aagcaatggt ataatcacaa tatgaatata gaaatggggg aaaacatttg gatacaaaag
     4681 aagaacagct acttattgat ttaacttctg caaaaggagt acctggaaat gaagaagagg
     4741 taagagaagt attcagagaa tatgccaaac cattcgctga tgatattttt tatgacggtt
     4801 taggcagtgt gattgccaaa catggtgctg gcggaggccc caaggtcttt atttctggac
     4861 atatggacga agttggtttt atggtaacaa aaataaccga aaaaggattc ttggaattcc
     4921 aaactcttgg aggttggtgg gggcaagtca tgcttgctca gcaagttgaa atcaaaacac
     4981 gtgaagggaa aatcgtccac ggtgtgattg gttccaaacc accacatgtt ttaacacctc
     5041 aagtaagaaa taaaccttat gaaataaagg atatgttcat tgatattggc gcatcaagcc
     5101 aagaagaagc caaagagtgg ggcatacgtc ctggagatat ggtcactcca tatattgaat
     5161 acaaacgaat gaatggcagt aaatatttac tggcaaaagc atgggataat cgtatcggga
     5221 cagctgtttc tctgcgagta ttagagaatc tatctaaaga agcacaccca aatgttttat
     5281 tcgcaggaag cgatgtgcaa gaagaagtag gcttgcgagg tgctcgaaca agtacacacc
     5341 ttgtaaatcc cgatattgct tttgctttag atactggtac agcaggagac actccaggaa
     5401 tgacaccaaa agaagcagat tcgattttag gaaaaggccc acagattttg atttttgatg
     5461 catctatgat cccgcataaa aaattattga attttgttat tgatgtagca gaggaaatgg
     5521 atattccatt ccaatataca gtgatttctg gcggaggaac tgatgcagga caaatgcatt
     5581 tgacaagaga tggtgttccg tctgtagcca ttaccgttcc tgttcgttat ctgcattccc
     5641 atacatcagt gattcatgaa gatgattatt tgaatacagt gaaactagtc acagaattag
     5701 tgaaacggct tgatagtgaa acagttgaaa aaattcggag ttatgaataa tgaataatag
     5761 aatacctatt tctgaatatc ggaaaaaagc acgagtttct ttggaaaatc aatggggaat
     5821 caatacttgg cttgttttct tatctataat atttacgagc gtcatccaga acttttttga
     5881 cggtttcagt ccttttccgg aagaaagcat gcagcagcac ttattgagtt ttctagtcga
     5941 taatttactt ttattcgcat tcaactatgc attgttttac attgcgctag tagttgtacg
     6001 gggtggcaga ccaaaaagta accttttatt ttcagttttc caaagaaagt attttggttc
     6061 gatacttggc atcaatttga tcaattcagt catcaactgg ctactcaaca gtttaatctt
     6121 tctgcccatg tttttaatca gcggagtgag tacttatatg cagctgcttt tcagttcaaa
     6181 tgcggtacct acgggtgtca tgccagaaga ttttcttgat atcagctttg ttttatcgat
     6241 gatagggatg atttttgttt cagcactcat catgagtatt gttggcggtt tattccaatt
     6301 tgctgcatgg acaaagttcg actatcctga tttgtcggtt attcaatgtt tgaaatatgg
     6361 ctggttttta ttgaaagata gaattggtac gtatattttg ttgcagctgt cttttatcgg
     6421 ctggtatttg ctaggagcaa tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt
     6481 gaatgtgtca gttgcagaat tttatgaaca agcacggatt gaaaaaggag gtccagacgc
     6541 ctattttact tagcatgtaa gcagtttatt catcaaaaaa ataacttttg tggtaggata
     6601 aatctttgag ggggaatgta cgatgaaatt aactgtttta ggatgcttag gagcgtatcc
     6661 ttataaaaat caagggacaa caggttattt gcttcaatct gacgggttca atttgttgat
     6721 cgatgcaggt agtgctacgc tgatcaaatt acaagaacac cttgatccac ttgatctaga
     6781 tgctgtgatc cttagccatt atcatcatga tcatattgct gaccttggtg tattacagta
     6841 ttactggcaa cttcatccta agagaaatcc ggagacattg ctttcaattt atggacacac
     6901 aaaagattcc attcactttg aagaattgac gttggaaaat gtctcaaaag gacatcctta
     6961 ttttgaagct gaagaattaa aaattggtcc ttttctgatt acttttatgg aaacaatcca
     7021 tccagtagta tgttacgcca tgcgtatcgt agaagaaaca acggggaaag tattcgtatt
     7081 tacaggtgat tcaggttatt tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt
     7141 tctagcagat acctatcttt ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa
     7201 agaagctgga gaacttgcga aatcagctaa cgtaaagaaa cttgtactta cacacctacc
     7261 tcagtttggt gatttagaac aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat
     7321 tgatatagaa cttgctgcag ttgacaaagt gtttaacatt taggaggaag aacatgattt
     7381 ttgtaccaaa tgaaaaccac gacccaagaa tcaatttagc aattgaacaa tatttgctgc
     7441 aagaaatgcc tttagatgaa cctattttgc tcttttatat caatgaacct tcaatcatca
     7501 tcggcagaaa ccaaaatacg cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca
     7561 ttcatgttgt ccgtcgctta agtggaggcg gagctgtcta ccatgatttt ggaaacctga
     7621 actttagttt tatcatgcct gatgatgggg attcattccg tgactttgcg aaagtaacac
     7681 agccaatcat ccaagcactt catgaattgg gtgttgctgg ggcggaattg aaaggccgaa
     7741 atgatctagt cattgatggc atgaaatttt ctggcaacgc gatgtatgct accaacggtc
     7801 ggatgtttgc tcatggaaca attatgttcg actctgatat caatgaagta gtcaatgcat
     7861 taaaagtaaa aaaagacaaa atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa
     7921 cgaatatcaa accatttctt ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc
     7981 aagaaatctt gttgaaaatt ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat
     8041 tgactgatga agattggaaa aaaatcaatc gaatctctga ggaatattat cgaaattggg
     8101 attggaatta cgggaagtct ccagatttca atttctctcg ccaaaaacgt ttttctatcg
     8161 gttcgattga agtccatttg aatgtctcag aaggaatgat caaagaagca aaaatctttg
     8221 gcgatttctt cggactagga gatatctctg acgtggaaaa aaaattggtt ggacagaaat
     8281 atgataaagc atctctatca aaaatagtca gccaaattga tgtgaagaaa tattttggtg
     8341 cgatagaacc agaagaattg cttaccttac tttattgaaa ataaaaaata gcggaatcca
     8401 catatatgga ttccgctatt ttttatacag gattcgggta atttcgagag agttgaacag
     8461 tgatccgatc aatgaaagta tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta
     8521 cgtctttcat catcattaag tcaaaacgtt cctgatcatc taattcttct gtgatgcctc
     8581 gactaaatag gtttgtatcg taatgtgcac tatgttcgca acaaatgacg aaatgttcct
     8641 cttgttcgtg tatcgacaaa taaatttttc gatacagtgg attttcgatg gcatgagttt
     8701 ctctaaagga atgctctaaa gcgtggatca aaagttgagc cagatcgtca tcgttgatag
     8761 gaagttcttc gggaagatct gcttggaagg ttatctgaat attccgtttg gcagctaact
     8821 tctggtaacg cgctaagata agatttgtca cctggtggtt gcaaaaagaa cacagcattt
     8881 cgaaattttt tgcttcttct aatatctttt ccacatagtt tttagctttt gtttggtccg
     8941 tatgcattaa atcttgcaga ttctccatct cttggaca
//
LOCUS       DACENJ010000107         8966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.110, whole genome shotgun sequence.
ACCESSION   DACENJ010000107 DACENJ010000000
VERSION     DACENJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.110"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            599..1240
                     /locus_tag="IW748_001755"
     CDS             599..1240
                     /locus_tag="IW748_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ1104665.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLNFRNALLGSDIGNSSLESIEIEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYELTIV"
     gene            1359..2444
                     /locus_tag="IW748_001756"
     CDS             1359..2444
                     /locus_tag="IW748_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1104666.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSKEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            2441..4333
                     /locus_tag="IW748_001757"
     CDS             2441..4333
                     /locus_tag="IW748_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1104667.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            4340..4717
                     /locus_tag="IW748_001758"
     CDS             4340..4717
                     /locus_tag="IW748_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1104668.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            4868..5644
                     /gene="ant(9)"
                     /locus_tag="IW748_001759"
     CDS             4868..5644
                     /gene="ant(9)"
                     /locus_tag="IW748_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)"
                     /protein_id="HAQ1104669.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETS"
     gene            complement(5717..7264)
                     /locus_tag="IW748_001760"
     CDS             complement(5717..7264)
                     /locus_tag="IW748_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1104670.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(7366..7719)
                     /gene="tnpB"
                     /locus_tag="IW748_001761"
     CDS             complement(7366..7719)
                     /gene="tnpB"
                     /locus_tag="IW748_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1104671.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(7709..7903)
                     /locus_tag="IW748_001762"
     CDS             complement(7709..7903)
                     /locus_tag="IW748_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104672.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(8098..8829)
                     /gene="erm(A)"
                     /locus_tag="IW748_001763"
     CDS             complement(8098..8829)
                     /gene="erm(A)"
                     /locus_tag="IW748_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAQ1104673.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(8887..>8966)
                     /locus_tag="IW748_001764"
     CDS             complement(8887..>8966)
                     /locus_tag="IW748_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
                     /protein_id="HAQ1104674.1"
                     /translation="WKGDKSMGTFSIFVINKVRYQPNQN"
BASE COUNT         3070 a         1431 c         1671 g         2794 t
ORIGIN      
        1 gtgcatctcg ccataaacat tttctaaagc caccgcaaac cgtcctagcc atcgcctagt
       61 ccgattttgc ttgatctgtt ccatttctgt ttatttagtg ggtcaacacc gtaaccttaa
      121 atagccatcg cctatttaag attactaggt cttctttgaa ggtaaaaagt ctttcattct
      181 tcaagacttt taggctttca agctacgcct gaaagcattg gaattcatcg ctctttattt
      241 ggttttgcca tttataattc cttacgccca ttatctttct cgcctgtcat accttgtcag
      301 aagtgtttag agcttatctc atctttggat taatacaagt ggcgtaaatt tttttctaaa
      361 cgcaagcgtt acgaaaataa tttacttaaa cacttgtatt aatccggcag ataagaacgc
      421 attctaatca ctcccgacaa ggcaagacag gcgaaaaaga caaataaatg aacgaaagga
      481 agaaacataa aatgcaaaac caaatagcga tgaagaaaaa accacctccc tctaaaacaa
      541 tccaaacaaa aaagaatttt gaaaaaccaa aaatcaaaaa cagaaagaag cgttcaacat
      601 gagagataga attttagaag ttaatcaaga aatggtaaac aagttaacaa atatcagtgg
      661 gaactatgaa aagtacgatg gtactttact tgataactat atttttcacg atacacaaaa
      721 tatcagaatt ggcagaagta aaccgagagc gttcattctc atcaaagaga aatatttgaa
      781 tgaatggaat agcacctatg aaatggttat gactgataac tttgaaaagg tattgaattt
      841 cagaaatgct ttgttgggtt ctgatattgg aaatagcagt cttgaatcta ttgaaattga
      901 aaataagacc gagaatgaag gtgaaggatt gattaagaat gtcttgaaaa tcgaacaaaa
      961 atatatcagt gaagatagaa gtttttatag agtcacaagc tcggttggtc tagcaaactt
     1021 tgctactaac ttaattggaa acaatgctag tgaatctttg ctagtgatgt gtctgaatac
     1081 aaagaatgag gtcattgctt atagcgaagt tttcaaaggt ggaattaatc aatcagtagc
     1141 gataccaaga gagatttttc aactagcttt gctaaacaat tcagcaaaaa tcgcagtatc
     1201 acataatcat ccgagcggat atgaattaac catagtataa taaataatag tagttaaagt
     1261 agtttaagag gagatattaa agggatattt tactttaaat aaaaattaac aaggtttata
     1321 atttgtagta gttaaagtat ttaaagaggt gggaacatgt gaaggttcaa aggatagaag
     1381 tggagaataa gccgtatcca ttgtatttat tactagataa agaataccag ctaatagaac
     1441 cagtaatgaa atttattaaa tacttagata atactggtaa gtctcctaat accattaagg
     1501 catactgcta tcatttaaag ttgctgtacg agttcatgga acagagaggt gttattctta
     1561 atgatattaa ctttgagttg ttagcagact tcgtaggttg gttgagatat ccttcagcat
     1621 caaatgtaat tgatcttcag tcaaaaaaag ccataagaga agaaacgaca gtgaatacaa
     1681 ttttaaatgt agttatgagt tttcttgatt atttaagtag attaggagaa tttaaatcaa
     1741 ttgatgtatt taaacaagca aagggaagaa atttcaaagg atttttacat catgttaata
     1801 agggtagata ccaaaagaat gtcttaaagt taagggttaa aaagaaacag ataagaacat
     1861 tgagatcaaa ggaagttaag caaattattg atgcttgtca tacgaaaaga gataaattaa
     1921 ttttaatgct tatgtatgag ggtggtttaa gaatcggtga agtgttatcg cttaggcttg
     1981 aagatattgt cacttgggac aatcaaatcc atttaacacc tagagatgtt aatgttaatg
     2041 aagcttatat taaattaagg aaggaaagaa caatacatgt gagtaaagaa cttatgtcac
     2101 tttatacaga ttacttgata tatgagtata gtgaggaatt ggagcatgat tatgttttta
     2161 tttccttaaa agaaggctat tttgggaaac cactaaagta ccaaagtgtt cttgatctag
     2221 ttagaagaat agttaaaagg actggaatag aatttacatc acatatgctt cgccacactc
     2281 acgcaacgca gctaattagg gaaggatggg atgttgcgtt cgttcaaaag agattaggtc
     2341 acgcacatgt tcagacaacg ttaaatacct atgttcatct ttcagatcag gatatgaaaa
     2401 atgagtttaa taaatacctc gagagaaagg agcataagaa atgaatgctt ctagtaaaag
     2461 gaaaattatt agtcagagtg agattagcaa aaaaatagct gtaatgaatg aagaaatgca
     2521 ggggttttgg gctaataata gttgggatat aagaaaatgt ccacatcctt ctgccataga
     2581 attaagtaag aatcctgctt taaggaatcg ttgggttcgt tttgaacgtg ttaaaaatct
     2641 gtggttaaga acagaattga aatattttta tttttaccat ttaaacaatg gaatatggaa
     2701 tgcaaaaact gtctggatta gaaaaggaac agtaattaat aaaatgttag atttcttaga
     2761 tttaaagtat cctagcatta cttcaattac tgaagttcct attgaaaaag caatgacgga
     2821 gtatagaact tatttgacaa aacggggtgt tagaattacc actactaatt ataagattac
     2881 tgctaatcaa gaaaaaacac ctgtaaaagc taattcctac tatgttacta atctaaaaca
     2941 atttatggag ttttatgaga acttttattt tgatggagag gagtgggata aagacgtttg
     3001 ggatagacgt aacttacctt tgccagatga taaggttaac ccaacacaat atgaatatac
     3061 aattaacttt aaagggtttc ggaatacata ttttaaacaa cttgtaaaaa gatattgtaa
     3121 gttgagattg aacgtggata gcttttccta tgtaagtgat attgcccaaa gacttaaaga
     3181 gttctttaat tttctggaca tgaaatttaa acaagttcag agagtacacc aattaacgag
     3241 agtggaaatt gaagcatatt taagtgaact aaacatgatg ggaataaaac ctagtacaat
     3301 aactgggagg atctctatat tggaaggact atttagtacc cttcttaggc tagaatggga
     3361 tgatgttcct tccaaaatat taatttattc tgaggactat ccgaaaatac caagagcaaa
     3421 accacgcttt atagatgaat tcgtcctaga gcaattgaac agtcatcttg ataaattacc
     3481 cgaatatata gctacgatga ctatgattgt tcaagaatgt ggaatgagga taagtgaatt
     3541 gtgcaccttg aaaaaaggct gtctattaga ggacaaagat ggagatttct ttttaaagta
     3601 ttatcaatgg aaaatgaaaa aggagcatat agttccaata tctaaagagg tagctttact
     3661 tattaaagtt cgggaagata aagtttcaga ggaatttcca gatagtgaat acctctttcc
     3721 aagaaaagat ggatcgccat taaaacaaga aacatttaga ggtgagttaa ataaattagc
     3781 ttatgagcaa aatatagtgg ataaatcagg tgagatttat agattccatg cccatgcctt
     3841 tcgccataca gtaggaacaa gaatgattaa caacgggatg ccccagcata ttgtgcagaa
     3901 atttttgggg catgaaagcc cagaaatgac aagcagatac gctcatatct ttgatgaaac
     3961 tctaaaaaat gaatttacta aatttcagga aaaactggtt accaataatg gagatgtgct
     4021 tgatctagat gaagataatg aagtcgatga tgtagagctt caatggttca agaaaaatat
     4081 aaatgcacaa gtgcttccaa atggttattg tagattgcca gtagtagcag gtggttgtcc
     4141 acatgcgaat gcatgcttag attgcactca cttctgtacc agtaagcaat tcttaccaca
     4201 gcacgaagaa cagttagagc gtacagaaga gttattagcc atagctaagg ataaacaatg
     4261 gcaaagacaa gtagagacta atagccgtgt taaagagcgt ttagaacaaa tcattggaag
     4321 tttgacgggg taattatcaa tggataaaca agttagaaat acaacagaaa ttgtacgttt
     4381 ggcgaagcag aaatcaaaaa agacaaggga aaaagtagac aaagcgattt ctaaattttc
     4441 gattgaaggt aaagttatta attttaattc aatagcaaag gaagctaatg tttctaaatc
     4501 atggctttat aaggaacacg atattaggca aagaatcgaa tcccttcgtg agcgtcaaat
     4561 aacagcaaat gtagtctcaa aacccaagaa aagttctcgt tcggaggaaa tccttattaa
     4621 aaccttaaaa agaagagtaa tggaattaga aaaagaaaat aaaaaattac agaaccaaat
     4681 tcaaaaatta tatggagatc tgtataataa agaataatta ttaatctgta gacaaattgt
     4741 gaaaggatgt acttaaacgc taacggtcag ctttattgaa cagtaattta agtatatgtc
     4801 caatctaggg taagtaaatt gagtatcaat ataaacttta tatgaacata atcaacgagg
     4861 tgaaatcatg agcaatttga ttaacggaaa aataccaaat caagcgattc aaacattaaa
     4921 aatcgtaaaa gatttatttg gaagttcaat agttggagta tatctatttg gttcagcagt
     4981 aaatggtggt ttacgcatta acagcgatgt agatgttcta gtcgtcgtga atcatagttt
     5041 acctcaatta actcgaaaaa aactaacaga aagactaatg actatatcag gaaagattgg
     5101 aaatacggat tctgttagac cacttgaagt tacggttata aataggagtg aagttgtccc
     5161 ttggcaatat cctccaaaaa gagaatttat atacggtgag tggctcaggg gtgaatttga
     5221 gaatggacaa attcaggaac caagctatga tcctgatttg gctattgttt tagcacaagc
     5281 aagaaagaat agtatttctc tatttggtcc tgattcttca agtatacttg tctccgtacc
     5341 tttgacagat attcgaagag caattaagga ttctttgcca gaactaattg aggggataaa
     5401 aggtgatgag cgtaatgtaa ttttaaccct agctcgaatg tggcaaacag tgactactgg
     5461 tgaaattacc tcgaaagatg tcgctgcaga atgggctata cctcttttac ctaaagagca
     5521 tgtaacttta ctggatatag ctagaaaagg ctatcgggga gagtgtgatg ataagtggga
     5581 aggactatat tcaaaggtga aagcactcgt taagtatatg aaaaattcta tagaaacttc
     5641 gtaagcgccc catagaacag tacctgtacg tcaaataacc cgacctatag aatagaatcg
     5701 ggaatgtttg gcgtttttag ttacgagaaa aatgttgttg tacatccttg ttccaaggca
     5761 acagttgatc taaagtttct gctgaaagta cgggaagatt tggtatctta tcaaatagaa
     5821 actctagata ctttacagga ttcaatccgt taagttctgc tgttttatat acactcagaa
     5881 tgattcctga agttcgtgct ccttcaagac ttgtggaatg caaccaattt ttccgtccta
     5941 tcaccagctc tttgatattt cgctccgcac gattatttgt gagttctaaa cgaccatctt
     6001 caaaaatagg ttcaaaacca cttcgtagtt tacacgcata ttgaacggct gcatccaact
     6061 tggattttgc aacaggatct atcgttgcca tccactcata aaaagcaata aataatggac
     6121 gcagttcttg ttgccgacag cttaatcgct tctcaggaga aaagtctttc catttctttt
     6181 ctaggacaaa ccatttatcc aattgtttga ctgcttgagc tgcgggaata tccgtgtttt
     6241 tctttccttt tggaattgct tcgaaaaatt tccgccgaac atgcgcgaaa caatagacaa
     6301 gtcgtacact ttccacggca ttatacccgc tatagccgtc acagtgtaaa taccctgtaa
     6361 actccttgag aaaagttttc ggaacatctc cagcacgact ttcgtcgtgg tgataataaa
     6421 cgatgggatg ctgactgtct tttcctgtac aaaactgcca gtaataggta tctgttttgg
     6481 cactttcaat cacacgataa cttgtttcat ccccgtgtag aatctcttct tgattaagat
     6541 agactcttaa tcgttctcct aaagggtcta acgcgttttg cactgcttta atatgccaat
     6601 tggtgatatt atctctcgaa atatccaacc catgtgtttg tttccaatag ttctcttgcc
     6661 gataggcagg aactttctga ccaaatttca tccggatcgt ttccgccact aaactattgg
     6721 aacctaagct attagaaatc aaaggtttag gaacgatcgc ttttttaaaa gaagaaagtc
     6781 cttctttttc gcagctttta caacaatagg tatgttgata atgggtgaga caatccaaca
     6841 tagcctgatg gaaacgaatt tcttctcgta cttttgtccg tccgatatct ttcatctgtg
     6901 aaccgcaatg ttcacaaaat tgttcttcca aaggaaggag atgttctttc tctgttatag
     6961 gtaagtgttt gattttgtct gctttcgttc ctacaactcg ttgatgtcct tttactggaa
     7021 taatggtggc agctgcttct ggaacactta cagaaatttc ttcatcacta aaaagattca
     7081 attgcccatt cgtttcctca aagagagtct ctttagagcg tccaaataat ttgttggtta
     7141 aataattgac cttttcattc aatagttgga tttcagttgt aagattctga atggtttgtt
     7201 gttgtgcttg attggtttta tgggcttctt gaagcaactg aagcaacgta tctgtagttt
     7261 ccaaaatttt tcaccacctt gaatcgaact gattttgtta cagaatacca caagaaacgc
     7321 acaagaaata ggggttcaac acattttttg tgttaacccc ccagcttaaa aaactccttt
     7381 ttttcctggc aaaatagctc gaggttgatc gatacttaat ccttctaaca accatcgaac
     7441 ctgttggttc gttaaggttc gaacttcgtc tttcgtccga ggccatttta acttgccgtt
     7501 ttctatccgc ttataacaga gaagaaaacc atcaccgtcc caatagagaa gtttgaaacg
     7561 gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg taaagctcga gttgatactc
     7621 ttgttgaata agactggcaa gaccatcaat tccttttcgc aaatctgttt ttccgcacac
     7681 aatataaata tgtttggttt gaagtaaatc ataaagcatt ggacaacagt tctttcaaaa
     7741 tagcttgaag aatataattg ttaataccgt tatagatcaa aacttcagaa ccattctctc
     7801 ttttgatgcg catcgctaac ttggcagccg cgggtttacc tttttctggt gcactctttt
     7861 cgtttcctaa ttggacagga acaatctcat ttaatttata catagaaaac ctccagactt
     7921 cttgatagct caagtatatc ttgaggtgca tttgttagta aggtactgtt ctatggggcg
     7981 cttaccaata acaggtgctt acttttaaaa ctactgattt attgataaat attgaacaat
     8041 ttttgggaag aataaagcgt cctcttgtga aattagagaa cgctttatta ctttaattta
     8101 gtgaaacaat ttgtaactat tgaaaataga aagaaattgt tccttcgata gtttattaat
     8161 attagtgaca tttgcatgct tcaaagcctg tcggaattgg tttttagtga aaagaacacg
     8221 atattcacgg tttacccact tataaacaaa agatcgatac tttttgtagt ccttctttga
     8281 aatcaatggt tgatgtcgtt caagaacaat caatacagag tctacacttg gcttaggatg
     8341 aaaatatagt ggtggtactt ttttgagcat ttttatatcc atctccacca ttaatagtaa
     8401 acccaaagct cgttgcagat tttgcaatct tttcgcaaat cccttctcaa cgataagata
     8461 gctatattta gcctgacttt caaaggtaat tcttttgaca atatccgtac tgatgttata
     8521 aggaatatta ccatatatct tatagtttat atgttttggg aaggaaaatt ttagaatatc
     8581 cgtttgaatc acttttatat tctcagaggg gtttaccgct tctttagtca cttgacataa
     8641 gcctccatca atttctatag cagtaactga tcgactcatt ttgactagct ctttggtaaa
     8701 atgtcctttt cctgatccga tttctattac gttgtcttgt ttactgatat tcgtgtgatt
     8761 caatatttct tttacatgct ttttagaagt aataaaattt tgcgtgtctt tagggttttt
     8821 ctggttcatt ataaccttct ccttactggt tataatgaac tgacattaag cgttcattat
     8881 aaccaattaa ttttgatttg gttgataacg aactttatta ataacaaata tagaaaaagt
     8941 acccatactt ttatctcctt tccatt
//
LOCUS       DACENJ010000108         8945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.111, whole genome shotgun sequence.
ACCESSION   DACENJ010000108 DACENJ010000000
VERSION     DACENJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.111"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..368)
                     /locus_tag="IW748_001765"
     CDS             complement(<1..368)
                     /locus_tag="IW748_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104675.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFL"
     gene            complement(665..3046)
                     /locus_tag="IW748_001766"
     CDS             complement(665..3046)
                     /locus_tag="IW748_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1104676.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3036..3683)
                     /gene="recU"
                     /locus_tag="IW748_001767"
     CDS             complement(3036..3683)
                     /gene="recU"
                     /locus_tag="IW748_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1104677.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3747..4286
                     /locus_tag="IW748_001768"
     CDS             3747..4286
                     /locus_tag="IW748_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1104678.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4376..4795
                     /gene="gpsB"
                     /locus_tag="IW748_001769"
     CDS             4376..4795
                     /gene="gpsB"
                     /locus_tag="IW748_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1104679.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4817..5184
                     /gene="rnpB"
                     /locus_tag="IW748_001770"
     ncRNA           4817..5184
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW748_001770"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5393..6559
                     /locus_tag="IW748_001771"
     CDS             5393..6559
                     /locus_tag="IW748_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1104680.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6605..8101
                     /locus_tag="IW748_001772"
     CDS             6605..8101
                     /locus_tag="IW748_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1104681.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8182..8865
                     /locus_tag="IW748_001773"
     CDS             8182..8865
                     /locus_tag="IW748_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1104682.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2860 a         1595 c         1868 g         2622 t
ORIGIN      
        1 aataaaaaaa taccatttat aactaaacat aaactaatgg atttgggcgg tttcaggctc
       61 atcagaaaaa tatagattct ggtgaacaac aaccataaaa aagtgagaat cattgcatga
      121 gagcttcttt ccataaattt tcctttaaga gtagtgctta aaagtacaga aaaaataata
      181 aatgcaagga taggagcaat taaagtaaat ttatttaata ataaacaaaa tttttcagct
      241 tttttattct tggaataacg aggtattgtt ttcgataaaa aaagatggtg cagacaaatt
      301 atgataatta agcatactat cagataactc aaaatgaagc cttctttcaa aaaattattc
      361 aaaaccatat attgtatttt ttatttgata ataacaatgg gattattaag aagtggcact
      421 aaataaaaga ctagtgtgta tttagccgat ttaattataa acaagtaaat ggataaagta
      481 tatgtaatga taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca
      541 tgtatatgct ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat
      601 ttgtagctgt tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt
      661 tttactatct gtttccagaa gctgaggtgc tgctggatgg tgtatggtca tttgcagcat
      721 ccgctccgct agttgctggc tcactggaag aagcaggagg agcagatgaa gacggtgtcg
      781 agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg
      841 ctgtcgtgct ttcttttgag gactcagcag ttgattcgct ttgactagta gagctagagc
      901 tttgagtcgt tgtctctgtt gttgagcttc caggagtctg gacatagctg gaagatggaa
      961 tagttgttga tggtgtaatt gcattcgatc tagctgtata ttgatctttg tagtacagtt
     1021 caccgcccac acgaattaat ccgcttggca tctcccagtc tgtatttgtc acgttagctg
     1081 aaacatactg cataagctcg cgataaacat cagaggctac gtgtgacgat tctgaagtga
     1141 caggtgtcat ttttttattg taacctgtcc acacggaaat cgaataattt ggcgtatagc
     1201 ccgcaaatag gatatctgga tacaccccag aagaaatacc aagttttgca tactcgtcat
     1261 ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa cccagcgatt tgcgcattcg
     1321 taccagttcc ttcagtgatc gtatctttta aaatgtctgt gatcatataa gctgtttctg
     1381 gagacatggc tgtttttcca tctggctcat aagtttcttc tgttccgtct tggaagacga
     1441 ttttattaac atattgaggt ttgtaataag tccctccatt ggcgaaagcc gcataggctg
     1501 ctgccatttt taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc gtattactag
     1561 agatcgcatt cgattgatgg atcgtactgt attcgatccc aagatttttt aagaaagaag
     1621 ccactttatc tgaaccgact tcattgaata atttgactgc tggaacatta cgggataagt
     1681 aaagtgcttg gcgtaaagta atggtgccca tatattgatt atcccagttt cctacaggag
     1741 tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt ttttccagta gaatatttca
     1801 aatattcaaa agctggtccg taatctgtga caggtttcat cgtagaacca aaatcacggg
     1861 aagtattcac tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa
     1921 tctgggcttt tactttcccg gtattggtgt cgataagggt ggaggcaact tgcatctcgt
     1981 cgtctgggta agagacatac tgatccgtgt tgacgatatc atatagtttc ttttgggcgt
     2041 ccagatccaa attcgtataa atctctaaac cgtctgtgta gacattctta tctgtttttt
     2101 cttgtacttc attgatgact tctttgacat aattatcgac gatcttagta ttgtcatcgg
     2161 attgagttag ttcttgcaga ccatccgtta ccggtacatt aacagcttga tcatattctg
     2221 tttgagagat tttttcgttt tgaagcatcg tatacaaaac agtatctcgt cttttcttcg
     2281 cctgatctgg ataaacataa ggatcatagg cggaaggggc ttgaggcatc ccagcaagca
     2341 atgctgtttg cggtaaagaa agttctgata atttcttacc aaagtacatc tcagaagctg
     2401 tctccatccc gtacaagccg tttgacatat acactttatt tacgtagtag gtcaagatct
     2461 cttgtttcga ttttttttgt tccaatctta cagccatcca cgcttcttgg gccttgcgtt
     2521 tcagagtctg atcctcggca ctggtggaga agaaggaaag tttgatcaat tgctgtgtca
     2581 aagtacttcc gccttgcagt ccgccagagg taaagttaga gagggcagaa ccgatgatcc
     2641 gaatagggtc aaccccgatg tgtttataaa aacggcgatc ttctaccgac acgatcgcat
     2701 cttctaatgt tttcggtaat tcattcgcag aaattttttc acgcttttct gcgcctaaat
     2761 cttcgaatag ttcgccatct tgtgtatata gtttggaaga tacggtagcg tctaattttt
     2821 tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa aagtcctaca cctgctaaaa
     2881 agaggataca gaaaaatgag agtattccta aaaggatctt gataaataat ccttttttat
     2941 gtgttccaga ggactttcca gaacctttgc cagaattctt cttcacactc tttttaggag
     3001 taggctgttt ctgtcgtcta gagcttctag tttgttcatt tgccattctt gctttctcct
     3061 tcaatagata atgttgataa atagaagtca accgcgtcca aataaggaat ccgcggagaa
     3121 ataccagttt tgatctctat tcctagttct tcgatcaaag ataaaggtag tgatttgcgt
     3181 cctttttcct tttgggcgtc ccagtaagca atcaaatgaa cactattcaa ataaaaacag
     3241 cgcttcaaag acgaaaacca taacaagaca aagcagaccc cattttgttc gaggcatgct
     3301 ttcatatgtt cgatttgatg ggcatggaag tttttaaagg gaaaagatgt tttgttcttg
     3361 gtttcttttg cttcaaagtc caagtaaaag cctcgataga cgccattata gtctgtagtt
     3421 gaggcttggg taaagtaggc ttccttgatc acagcagcac ttctcttagg atagtctact
     3481 ttcacgattt ggataggggt aggcttttta tggataaccg ccaacccgcg ggaaagataa
     3541 tatttattac tttcattgat tgcttcttca aagcgcatcc cccgattgcc gaattcagtg
     3601 atctgatttc ttttttcttg aattttttcg acgggagagg cattcttata ataaggttgg
     3661 ccatttggat agcgcaatac catctttaat cacactccta ggccacatta tagcaaaaaa
     3721 gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa agtaatctat gtgtctgggt
     3781 atcgaagttt cgaactcggt gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa
     3841 atgttttaaa aaaagaactc catcagttag cagaagaagg gctggaatgg gtacttgtca
     3901 gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg aaagaagaat
     3961 atccggaact atctcttggt attttgtatc ctttttctga ttttggtaat aactggaatg
     4021 aagggaacca agaaaaaaaa caattagcag aacaattagc agattatgtc gaagctgtta
     4081 gccatcaacc ctaccaatca cctagtcagc taaagaatca cactcgtttt atacttgagc
     4141 atacagtggg ctgtctgctg atctatgatg aagaattccc tggaaaaacg aagtattttt
     4201 ttgaagatgc acagaattac caagcagatc atccctacga aattcggctg atcagtatgg
     4261 acgatcttca aaattttagt gaataatcga tattgtgttt gatttatcaa cttttttcct
     4321 atacaatgaa aaaggtagaa atgttattac gtaaatgaaa actagaggtg taaatatggc
     4381 gaatttggtt tatagtccta aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg
     4441 ctatgatccc gttgaagttg atgaattctt agacaatatc atcaaagatt atgaaactta
     4501 cagcaaagaa ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa aagtagctca
     4561 attatctaaa actcaaggag cagctcaaac acgcgtgcaa caaacagagg caccaaagag
     4621 cgcagcagtg accaattttg atatcttaaa acgcttgtca aatcttgaac gtgaagtatt
     4681 tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca gcacaaccta atccaaataa
     4741 ctacacaaat gcagacacaa gcttagatga taatgaaaaa acacgtcaat tttaattttt
     4801 gattaatgaa tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt
     4861 ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc ataagctaag
     4921 gtactctttg gagtaacggc aggaaaacaa gctaaggctt cggctatgct taagtatcct
     4981 tgaaagtgcc acagtgacga agcggctggg gaaacccatc cggtggaacg cggtaaaccc
     5041 ctcgagcgag caacccaaac aatggtaggg gcgctttttg tctggaagaa gaacaggaca
     5101 aaaaggcagt ataactgcag atagatggtt accgctttgt tttttctccc tggaaaagca
     5161 aagtacagaa catggcttat agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag
     5221 taatgcgtta taagaaacaa gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa
     5281 tcgttcttat tacaaaacaa acttttgtat caccggttat tcggaatttg agtaaaagtg
     5341 tggggtagga ctgatgtccc acctcctttt tttataaata aggagattgt gtatggaaaa
     5401 aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa
     5461 agaattaaga gatcttggaa tcgaatgcca agttgagaac ggacgtgcac gttttgaagg
     5521 aacaatggaa acgatcgcta cagctaattt atggttacgt acagcagatc gagtaaaaat
     5581 cgtggttggg gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt
     5641 gccttgggaa gattttcttc cactagatgc agcttttcct gtagctggaa aatcgatcaa
     5701 gtcgaaacta tatagcacac ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg
     5761 tctccgtacc tattatcacc gtccagcttc tgtaccactg acggaaactg gcgcacattt
     5821 tcagttggaa gtagctttgc tgaaagacca tgtgatggta actctggata cgacaggacc
     5881 aagtttattc aaacgtggtt atcgcttaga aaaaggtgga gcaccattga aagaaaacat
     5941 ggcagcagca cttattatgt tgacaaattg gcgaaaagac cgtccatttt atgatcctgt
     6001 atgtggttca ggtacgcttt gtattgaagc agccttaata ggccataata tcgcaccagg
     6061 attcaatcgt gagtttgctt gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa
     6121 tgttcgatca gcagccgaag aaaaagcaga ttatgatatt gaattagata ttactggttc
     6181 agatatcaat ggacggatga ttgaaattgc caaagccaat gcagaagaaa tcggattagg
     6241 ctcttcgatt acctttaagc aacaagcagt caaagacttc aaaacggata aagaatacgg
     6301 ggtcatcgtg gctaatccgc cttacggaga acgtctagga gaagaagaaa ctgttcggaa
     6361 attatataaa gaaatgggcg atgtattccg tccattaaaa acatggagta aatatatctt
     6421 gactagcgac ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt
     6481 gtacaatggt gcattgcgta cggatctatt ccagtattgg ggaactcgtc cgcctagaaa
     6541 aaaagagaaa gacgaataat cttactaatt gattcattca attggatgtt tggaggaata
     6601 gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc tacaacaagc
     6661 tttaggaatc ctcgattggg atacacaaac aggtatgcct gaaaaagcta gtgcataccg
     6721 aagtgaagta gacagctatc tttacagcat ttatttttca aaaaaaatcg gtccaaaaat
     6781 caaagaagcc attcgctatt tttctgaaca tccggaagaa ttgtcggaag ttggaactgc
     6841 cgtttttgcc aaagtaaaag aagagtatga tttggaacat cgcgtaccgg aagatcttat
     6901 gagaaaatat acagctgccg tttcttcagc acacggacaa tggcaaaaag ctagagaaac
     6961 acaacaattt tctgattttc aacacgcatt gaatcaaaat attgaattaa caaaacagtt
     7021 gataccttac tggagaaaag atgaaaagac gaattatgat gttttgctga atcaatacga
     7081 acccgggatg actgtcgaaa tccttgataa ggtatttgat cagcttaaag aaggaattct
     7141 ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct gaaacagact ttttaaatcg
     7201 gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac aacttggtta
     7261 tgattttagt cgtggtcgtt tagatgacac tgtccaccca tttatgactg gaattaatcc
     7321 gaatgatgca cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg
     7381 agtgatccac gaggctggtc atggtatgta tgaacaagac atcgatgata aatatgcgta
     7441 tacaccggtt tatgaaggta catcaatggg tatccatgaa tcacaatcgt tgtttaacga
     7501 gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg
     7561 tgcagaagga acattcgatg atattccttt tgacaaattt tatcgttcat tgaaccatac
     7621 aaaatcaagt ttggttcgta tcgaggcaga cagtttgact taccctctcc atatcatcat
     7681 tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg aactacctga
     7741 aatatggaat caaaaatatg aagagtatct gggaatacga ccaacaaatg atgtggaagg
     7801 catcctacaa gacgttcatt ggtcaggtgc aagctttggt tattttccct cttatgcatt
     7861 agggtacatg tatgcggcac aattacttca ttctttgcag caagaaatag atgtggaaaa
     7921 tgttctagcg tcagacgatt attctccaat caaaaactgg atgtcagagc atatccatca
     7981 atatggtgca tctagaaaac caaatcaatt aatcatggat gcaacacatg aaccgcttaa
     8041 tccgcaatac ctgatcgact atatgaaaga cctgtatttt aatgtatatc aggtaaaata
     8101 aaatacaatt tacaaagata gctgaaattc atctaatcta tagatgtttt cagctattta
     8161 cttaagaaag aagtggaacg aatggataaa ttacaagtga tcgctgatta tagtatggac
     8221 caattagcgc atgatcaaac aggacatggg agcgatcata ccaaaagagt tgtcaagtta
     8281 gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct
     8341 tacctacatg atacgattga tgataaagtc gttaaaaatg agaacgaggc aaagcaacag
     8401 cttcgtgtat tccttcgctc tttgccaata gtagaggaac agatctcgat gattttttcc
     8461 attatcgaaa atatgtcctt ttctaaaaat ctctctgaag cggccgaact atctctggaa
     8521 ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg cattttgcgt
     8581 actgcttatt tcggcggtgg acatgggcac ccgatttttg atcctgaact gtatccgcaa
     8641 acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa
     8701 aaactgttct tgctgtctga taaaatgaat actgattacg gatatgaaga agccaaacga
     8761 agagaaacct tcatgcgtga attcttagaa gaattttttc tggaatggtc tattgacgat
     8821 gaaaatttta ctccagacaa ttggatcaaa aataacaaaa aatgatggag gttgtgacag
     8881 aagtggtcag ctccaagata tttcggatca ttttccgtaa aagttacttc tggaacactg
     8941 tttac
//
LOCUS       DACENJ010000109         8887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.112, whole genome shotgun sequence.
ACCESSION   DACENJ010000109 DACENJ010000000
VERSION     DACENJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.112"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            106..690
                     /locus_tag="IW748_001774"
     CDS             106..690
                     /locus_tag="IW748_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104683.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            701..946
                     /locus_tag="IW748_001775"
     CDS             701..946
                     /locus_tag="IW748_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104684.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            952..1674
                     /locus_tag="IW748_001776"
     CDS             952..1674
                     /locus_tag="IW748_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104685.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKDQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            1805..2548
                     /locus_tag="IW748_001777"
     CDS             1805..2548
                     /locus_tag="IW748_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104686.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2554..2919
                     /locus_tag="IW748_001778"
     CDS             2554..2919
                     /locus_tag="IW748_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104687.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            2935..3465
                     /locus_tag="IW748_001779"
     CDS             2935..3465
                     /locus_tag="IW748_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104688.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3627..4016
                     /locus_tag="IW748_001780"
     CDS             3627..4016
                     /locus_tag="IW748_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104689.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     SKLAQSYLEEKAGAIKDKEEMEKFAIE"
     gene            4009..4398
                     /locus_tag="IW748_001781"
     CDS             4009..4398
                     /locus_tag="IW748_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104690.1"
                     /translation="MNEETELFIKKVNFLVEKYFFTKHKIMYLIKGLIGGIILWILTF
                     FSLFGGFTNYKKLVDENVKQISASESLRHQAEDLVSNPLFIGGMVEVIIISFVIGYLG
                     MCIFQIIFDLIRGKVFQGFKSEKINLR"
     gene            4428..4652
                     /locus_tag="IW748_001782"
     CDS             4428..4652
                     /locus_tag="IW748_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104691.1"
                     /translation="MEQTNHITEETRKFICLESFYSEGRYCNKGETYTAYPIEGGFKL
                     VFENGDMNFTTELFECVVETWSDVLLEVTK"
     gene            4705..4965
                     /locus_tag="IW748_001783"
     CDS             4705..4965
                     /locus_tag="IW748_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104692.1"
                     /translation="MAYENLQLVAAGVSGDIYLAKAKDGIMDTNYRRVATDDVLSSAT
                     EWFRTNEESAVHFKGIDGNIHSLFYTKDKEKAKKILDILKES"
     gene            5304..7544
                     /locus_tag="IW748_001784"
     CDS             5304..7544
                     /locus_tag="IW748_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104693.1"
                     /translation="MSKYKFLDINVGSAFLINLDMFEAWAQSSGYNWAFSDDMIEFLS
                     RTMSWESYKVKQFLDVIAKPIYTSADDHWSGRELATLKELAEYNKNYNIPFQVSYTTD
                     DFSVIYALGKESRYLIPNRYLEIKMNSDYTNYTLGELKALRQSDSEEFSTLKNEVSTE
                     NFRISDIEELKKQQKQQLEKQKKLIEDIVNKRVDELAVQEAKIEKLRQELYEKQQSML
                     AEANKMRFELEKKVEELNTKIFYLETEIDAIEGYFGESTTFVQLRSGQKSLSEDPVVM
                     FQKVRYLDEELGRYFSMREVEGTADTMLTFEQALSSRDDLFDLFSPNKKSISMIRISK
                     SGYGYTNGQMESTNMLEKYKKYHGETLAILLRNGDNLWICWLDENKISFKDDLFYQNK
                     VTEQASETKNMVKEIDWTHTRNLEERLKDAKKQKKAVRGEFASRYILFSILQGVLDNG
                     TMMTLPEKVNVIQAINQKSPYFIASVADGWLEDTRLPSFSELIDKYSFYKETGEDRVL
                     EAEYELNPDKFENMRDYPHKQNDDIYVLYRLRGWSPTSANYSYYNSDNRSNSESGRDL
                     AYSIDLKDGIYQINLVKQGKFHKQMFSRTFVGVDSTTGENIYDYYYERETEYYIRGQK
                     AYNKSDYWGDKAKRLTANFRVYESEFINLTFLNSVFIDYVITTKRIGSYSSTYASLVP
                     MLNSMSRFLKEREEKELQYIKEIMPDFEMHEWQIALSDWKLTNKVRRITPFQAKRFLK
                     AMKFVK"
     gene            7682..8479
                     /locus_tag="IW748_001785"
     CDS             7682..8479
                     /locus_tag="IW748_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104694.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYGESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSKEEVRELVETPVRKKIETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYNSNRFDRLEKFCRPYTVLKTKKFNKTKRVS
                     IKLPTGQISLGEKDYLIKVGKELIIKVPERLFKENFEIRETLVWKEE"
     gene            8519..8701
                     /locus_tag="IW748_001786"
     CDS             8519..8701
                     /locus_tag="IW748_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104695.1"
                     /translation="MAVNRKLQVLITDDFGGAEWFDVQSIDFEEEVIVIENVYGEEYS
                     YSLEGENVEDLRLIGE"
BASE COUNT         3446 a         1123 c         1745 g         2573 t
ORIGIN      
        1 tgtttgtatg acaagtttac cataaaaaaa tattaaaaag cgagacaaat ttatgttatt
       61 atgttatcat gatagcataa atatatagag cgagatgatg gaaaaatgga tttgaaacaa
      121 tcgttttaca tctggttata tcctgataat cctatgtcaa taatggctga tatttttgta
      181 gttagcatct tattgtattt attttattta attttctttc acaatttaat cgggtacatg
      241 attcttgatt ggaaaggaat gttcgatata gagagaaaac catttgctaa caatcaaacc
      301 tataccgtct tcgaaagtgg agattgggaa gaaaatgaag ttgatgtgat ccatgaaatt
      361 ggattagctt tatcaggaat acaagatgct gatgttttgg tatcaatgac aacggatact
      421 ggatacaacg cttatgaggt attttatctt atgtatttta tatggcgtga tggtcaagaa
      481 attatcaatg gtcgtgctga aaaaacaatc atttctaata ctgaaatttc tttctgtatg
      541 ggtgaagggg aaatgattgt atataaagat gggtcagcaa acattaaaac agcctttggt
      601 gatattgaaa caagtattaa ggaacttagg gaatatgtgc agaacccata taattttcaa
      661 tatttagcag gaagaataaa tttagtttag gagtaaaaat atgaaaaaaa ttataaatca
      721 accaacaaat cacttgataa ttgaaggtgt tattttcgta acaatgtatt ttactgtagg
      781 aaatggcatc tcaaatacaa tcattcaaaa aaatccacaa tacctattat ttagtcttta
      841 tgcttggatt gtacaaatgg tttgggtttc catattgatg ctgttgtttt attggatagt
      901 acgggttatt cttttattgt taaacaaaag aaaaaaggat aggtgaataa aatgaaatca
      961 actaaattga aggttgtatt gttatcgaca gcagcattaa taacccttac agcatgtggt
     1021 acaactaata gtgctaataa taacaaagac acaaagtcta gtagtacgat ggttactaaa
     1081 aacagcacta catcggcatc aacaacaatg tcttcaatga ctgaaaataa tgctgaaaag
     1141 tatcaaaaat acgaagacat taataaagat caaaagccgg atgaagttta taacaacttt
     1201 attaatacgg taaaaaatga tgaaaattat gggaatgatc cattgcaaaa taaaaatctg
     1261 actatcgttc tttacaaacc tgattgtcca gattgtcaaa aagcagaatc aaaaatcatg
     1321 caagaaataa aagaaggatt tagttatgct ccaacattaa tgcatgatat taccataaat
     1381 aaaccagctt acgagtatta cgcagttgct gtagatatta ctgatggtgt acctgagtgg
     1441 gtacaaaaca ttcctggtct ggctgatgag aacaatcaat atcatacgcc aacagttgca
     1501 gtagtaacac caaagaaagt taacggcaca ctatactggg atgtaatggg aatgacaaca
     1561 aatacaactg atgaaatgat tgaaagtaca actaaatatt atatggattt gggagcatat
     1621 aaattatatc aacaatcaga acctgttatg gacgatcaat ccaaagttaa atagttcttt
     1681 agggcgtaag ccctattttt ttaaaattaa gtgataacaa ataaatgatt tctgaaacaa
     1741 acgttcttac tcaactactc gaaacacaca agctaattaa ctagattata aaggagaatg
     1801 cgtaatgaaa gaaatcagtg caaatgaagc tcatattccg caaaagaatc aggttggtat
     1861 atataaggtg cttcatggtg aaggaattta taaaatacag ttacttaccg gttacaataa
     1921 cggtgcaaat gggaagtggt taaaggcaac aagtattcgt gaacttagaa gtgaagtaga
     1981 aaaaattagg cttggtctca caagtgaaga aatagctaaa ctgaagggaa ataattcttt
     2041 tgtaattgaa aaagaaaagg aaaaacatct actttatcgt gagctgaaaa ttgaagaata
     2101 ctatgaaatt gatttacctg aaaaattaag aattattgat caacgttcag aagaaaaatg
     2161 gaaaactgaa atatatccaa gcttaaatga ctacaagaca tcaggaaagc ccatgtataa
     2221 ctacacaggc tttttaacct acacacaata caaatatgcc tttcgcaaat taaaaggcat
     2281 taaacaagct gaaattgccc tgtatgagat gaaaaaagtg gatagattga aattgattca
     2341 agatttagtg caaaatacat ggaatcaagc gattgtcgag ctagaaaaag aaaatgggaa
     2401 aattttaact aataaatatc tgatcgtaaa tgaaattgaa gaattaattg gtttattttc
     2461 atgtgaaaaa ggtgaagtct tcttgaagtc atcaccaacc tattcaattt tggacagtcc
     2521 gtatcagttt atacaggcaa ggagatgagt tagatgtcta gcttgaaaga tgactatatc
     2581 caagcaaaac aagagtatga aaaaatacag atgttgaaat ctgagaaagt cgaattattt
     2641 ttaacacaaa aattagcaga ggatcatatt gctaacttag atgctaaaac tttagaggaa
     2701 tatgatcagc agtttaatca gatacatcac aatatcattt tgcttgttga gcaggcaaag
     2761 cgaaggtttg aaaaagcgga atatgatcta ttagatcaag gaattgtatg cttggataag
     2821 ggattgcgaa gatattatgg tgcagagtta aaaaagcaaa atgtagatag aaatgcacta
     2881 aaacatgaaa tcttagaatt gataatgcaa cgaatttgaa ggggtagaaa gcaaatgtca
     2941 caaaacatta gtgctgtaaa acaattaata gaagaagatt tagagaccac agttcagaga
     3001 ttagcggaac gaattcattt tgcaaattat caaaccatct ctaactggtc aagcaggaac
     3061 agaaatccgg aagaaattcc aatgaaattt ttttatcgaa tcggaaaaga atatggtgtt
     3121 gatccgataa aaattttcga gaagaccatg atttattact atcatggaac acctaacgaa
     3181 gtgttagaac atcttttgga agaggcaaaa gaaaaatcat caatattaga gctatcgcaa
     3241 aatgacaaag ttattcaagt gaaatgcgtt gatccaacag gaaagattat tgtcggtttt
     3301 gatattgaaa gaaaatccaa aatagatttt attactcaat taaaaaagat aaataattta
     3361 gatattctaa cgcttagacc agatcatgaa cagttgttta tgagttttaa aaaacaattg
     3421 aaagaaatac aaaaaagata taacaaggtg tttcctaatg cgtgaaaagc tcaaagaaat
     3481 cagagttttt tggataatta acaaagaatg tatcatctta tatactcttg gatttctcag
     3541 catgatcttg ttttggtggt tatatttaaa acagtaattg gagaggggca agaggatttt
     3601 tatcaagata aggatgtgga aactgcatgt ggaataatag attaaaaaca ggattgttat
     3661 taatagttat ttcctgcgct atgatgattg gtatgaggat acaaagggaa caatcctatt
     3721 ttgaagtttc tgctaacaac gtaatagaaa aatgctatta tggtcaacat tattggagtg
     3781 aggaagtcag agaaaatata gatcgtgaat atgttcaaag aattgtttgg gatgcatatt
     3841 ctatcaaaga ttatcctaaa agtttaacga gtagattatt ttacagcgaa aaagataatc
     3901 aaaaactctc agatttgatg atgaaaaaag taagtaaact tgcacaaagc tatttggaag
     3961 aaaaagcagg agcaataaaa gataaggaag aaatggagaa atttgctatt gaatgaagaa
     4021 acggaattat ttataaaaaa agtgaacttt cttgtagaaa aatatttttt cacaaagcac
     4081 aaaattatgt atctaataaa aggattgatc ggtggaatta ttttatggat acttactttc
     4141 ttttctttat ttggtggatt tactaattat aaaaagttag tagacgaaaa tgtgaagcaa
     4201 attagtgcat ctgaatcatt aagacatcaa gctgaagatt tagtaagtaa tccattattt
     4261 ataggaggta tggtagaagt gataataatt agcttcgtta ttggttattt aggtatgtgt
     4321 atcttccaga taatctttga tctaataaga ggaaaagtct ttcaaggatt taaatctgaa
     4381 aaaataaatt tacgttagag ataataggct atgagaggag aagaatagtg gaacaaacta
     4441 atcatattac tgaagaaact aggaaattca tttgtttaga atcgttttat tcggaaggta
     4501 ggtattgtaa caaaggtgaa acttatacgg catatcctat tgaaggggga tttaaattag
     4561 tttttgaaaa cggagacatg aattttacca ctgagttgtt tgagtgtgtt gttgaaacgt
     4621 ggtcagatgt gttgcttgaa gtcactaaat aatcaatgtt atctagtaga attaagcaac
     4681 taaaaaagaa agaaggaaga aaatatggcg tatgaaaatt tacaattagt agcagcagga
     4741 gttagtggtg atatatatct tgcaaaagca aaagatggaa ttatggacac gaactataga
     4801 agagttgcga cagatgatgt gttaagctca gcgacagaat ggtttcgaac taatgaagaa
     4861 tctgctgttc actttaaagg aattgatgga aacattcata gtttgttcta tacaaaagac
     4921 aaagaaaaag ccaaaaaaat tcttgatatt ttgaaggaaa gttagttaat taactaacga
     4981 tataagagaa ggcgagacca agtatttgct tggtctctat cattttaacc actagtcaat
     5041 taaataaata tcaaaaatga gtatggctaa ccacttgatc acgaacagca tcgctacaaa
     5101 tactgtatca gtatttgata gaatacatat tctattttaa aaaaagaaaa gaaatttatt
     5161 gatattatac acatcggtgt gtataataaa gaggaagaag tgattagaac tttgagcaat
     5221 tcaatcattg ctttcgtagg agaataatag aacagctcct aagaaatccg ttgttcagta
     5281 aatttttgag taaaggagac gttatgagca aatacaaatt tttagacata aatgttggtt
     5341 ctgctttttt gattaatctt gatatgtttg aagcttgggc gcaatcatca ggatataatt
     5401 gggcatttag tgatgatatg atcgagtttt taagtcgaac gatgagttgg gaaagttata
     5461 aggtcaagca atttttagat gtcattgcaa aaccaatcta cacatctgct gatgatcact
     5521 ggtcaggcag agagttggca acccttaaag aactagcaga gtacaacaaa aactacaata
     5581 ttccgtttca agtttcttac actacagatg atttttccgt tatttacgca ttaggtaagg
     5641 aaagtagata tttaatccct aataggtact tagagataaa gatgaatagc gattatacca
     5701 actatacgct aggtgaatta aaagctctta gacagagcga cagcgaggaa tttagcacgc
     5761 tcaaaaatga agtaagcaca gagaattttc gcatatcaga tattgaagaa ttaaaaaaac
     5821 agcaaaaaca gcaactagaa aagcaaaaaa aattaattga agacattgta aataaacgag
     5881 tagatgagct tgcagtacaa gaagctaaaa tagaaaaatt gcgacaagaa ctctatgaaa
     5941 aacagcaatc aatgttagct gaagcgaata aaatgcgctt tgaattggag aaaaaagtag
     6001 aagagttaaa tactaaaatt ttttatttgg aaactgaaat tgatgcgatt gaaggctatt
     6061 ttggtgaaag tacaactttt gttcagttgc gttcaggtca aaagagctta tcggaagatc
     6121 cagtggtgat gtttcaaaaa gtacggtatc tagatgaaga attaggaaga tatttcagta
     6181 tgcgagaagt tgaaggtaca gctgatacca tgctgacttt tgagcaggcg ctatcctcac
     6241 gagatgattt gtttgattta ttctcaccga acaaaaagtc aatctcaatg atacgtattt
     6301 ccaaatcagg atatggctac actaacggtc aaatggaaag tacaaacatg ctagaaaagt
     6361 ataaaaaata tcatggagaa actttagcaa ttttactgag aaacggtgat aacttatgga
     6421 tttgttggtt ggatgaaaat aagatcagtt tcaaagatga tttgttttac caaaacaaag
     6481 taacagagca ggcatctgaa accaaaaata tggtaaagga gatagattgg acacatactc
     6541 gtaatttaga agagcgattg aaagatgcta aaaagcagaa gaaggctgtt agaggagagt
     6601 ttgctagtcg ttatattctt ttttcaatct tgcaaggtgt tctagataat ggaacgatga
     6661 tgaccttacc agaaaaagta aatgtgatcc aagcaattaa tcagaaaagc ccatatttta
     6721 ttgcaagtgt ggcggatgga tggttagaag acacacgctt gccgtcattt agcgagttga
     6781 ttgataaata cagtttctat aaagaaacag gtgaagacag agtattagaa gcggaatatg
     6841 agctgaatcc cgataaattt gaaaatatga gagattatcc acacaaacaa aatgatgaca
     6901 tttatgtgtt ataccggtta cgtgggtggt cgcctacatc agcaaattac agttattaca
     6961 acagtgataa ccgttcaaat agtgagagtg gacgtgactt agcatattcg attgacttga
     7021 aagacggcat ttatcagatt aatttagtaa aacagggaaa attccacaaa caaatgtttt
     7081 cccgaacgtt tgttggagtt gattctacta caggcgaaaa tatctatgat tactattatg
     7141 aaagagaaac agagtactat attcgaggac aaaaagccta caacaaaagt gactattggg
     7201 gtgacaaggc gaagcgatta actgcaaatt ttagagtgta tgaatccgaa tttataaact
     7261 taacctttct taatagtgtt tttattgatt atgtaattac tacgaaacgt attggaagtt
     7321 attcaagtac ctacgcatcc ttagttccaa tgttaaactc gatgagccgt ttcttaaaag
     7381 aaagagaaga gaaagagcta cagtatatca aagaaattat gccagatttt gaaatgcacg
     7441 aatggcaaat tgctttatca gattggaaat tgacgaataa ggttcgtaga ataacacctt
     7501 ttcaagccaa gcgctttttg aaagctatga aatttgttaa atagaaaaat ccaccagaca
     7561 agctgatgga aactccccca attactaaca gttgtaattg gtgttagctt acaagttaat
     7621 tatggtgttt agtcaatccc aaattgtgaa acgacaccga taattggaaa ggaaaaatga
     7681 aatgtctgaa gatttacgag agctgaagtt acgaaagaag cagtatgatc agtatttttc
     7741 cggtttgttg aataaacata ttgaagaagg acttgatcat gattttggaa aagatgaata
     7801 ttgtttgtat tgcgagcgtt acaatgaaaa aatgtctgaa tatctacagc taaagaaaga
     7861 gatacagaaa ttagagaaaa aagtacatgc ttggaagtat aaagattcca tgtctggtta
     7921 tggtgaatct caaaataagc caagaaaaaa gaagcaagcg tatgttctaa agatggtaaa
     7981 tggggaagag ttaattgtat tagcttacag taaagaagag gttcgagaac tcgttgaaac
     8041 acctgtcagg aaaaagatag aaacaataaa tgagatagac gacagccaat acaatagcat
     8101 ctatattgaa acaagtcatg gtggatataa gaaattagca actttcctta gaagttctaa
     8161 agttgggata atttctaaaa aagacataag tgctgttacc gatagttttt gtactttgtc
     8221 tgttcctaaa gcatattttc aatacaacag taatagattt gatcgattag agaaattctg
     8281 tagaccatat actgtattga aaactaaaaa attcaataaa actaaaagag tttcaatcaa
     8341 gttaccgact ggtcaaatct cgctaggcga aaaagattat ctgataaaag taggaaaaga
     8401 gttaataatc aaagtgccgg aacgcttatt taaagaaaat tttgagatac gtgaaacctt
     8461 ggtatggaaa gaagaataat agtttggaag acatagcagg gtataaggag gaattaaaat
     8521 ggcagtaaat agaaaattgc aggtattgat tacagacgac tttggcggag cagagtggtt
     8581 tgatgtgcag tctatagatt ttgaagaaga agtcatagtt atagaaaatg tatacggcga
     8641 agaatactca tattcgcttg aaggagagaa tgttgaggat ttacggttga ttggtgagta
     8701 gaaaatagca gaaatatcaa aatggagatg aaatcagcgt gtatatgatt gacaagaaaa
     8761 caggaaaacg tattttcagc agtaaagacg aggtaattcg ggatttgagt gaaaactttg
     8821 caccaattga attatttttc catcagttaa atctagaaat tgaaaaatta ctaaaggaac
     8881 ctacaga
//
LOCUS       DACENJ010000110         8649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.113, whole genome shotgun sequence.
ACCESSION   DACENJ010000110 DACENJ010000000
VERSION     DACENJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.113"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            79..669
                     /locus_tag="IW748_001787"
     CDS             79..669
                     /locus_tag="IW748_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1104696.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            761..2266
                     /locus_tag="IW748_001788"
     CDS             761..2266
                     /locus_tag="IW748_001788"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1104697.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(2350..3702)
                     /gene="celB"
                     /locus_tag="IW748_001789"
     CDS             complement(2350..3702)
                     /gene="celB"
                     /locus_tag="IW748_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1104698.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(3702..4220)
                     /locus_tag="IW748_001790"
     CDS             complement(3702..4220)
                     /locus_tag="IW748_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104699.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(4236..4562)
                     /locus_tag="IW748_001791"
     CDS             complement(4236..4562)
                     /locus_tag="IW748_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1104700.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(4575..6554)
                     /locus_tag="IW748_001792"
     CDS             complement(4575..6554)
                     /locus_tag="IW748_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1104701.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(6575..6889)
                     /locus_tag="IW748_001793"
     CDS             complement(6575..6889)
                     /locus_tag="IW748_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1104702.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            7057..7350
                     /locus_tag="IW748_001794"
     CDS             7057..7350
                     /locus_tag="IW748_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1104703.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(7428..8540)
                     /locus_tag="IW748_001795"
     CDS             complement(7428..8540)
                     /locus_tag="IW748_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1104704.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
BASE COUNT         2755 a         1714 c         1472 g         2708 t
ORIGIN      
        1 ttttgaattc ggtttcgttt ttttggtaaa gtaaagaaat atacagcctt ttatggctaa
       61 catttatagg agatatcaat gacacaagaa ataaaaaaac tacccgttat atttttaggt
      121 cttttcttta tcacagttgg attgaattgg ttcttgctcc ctcacgatat agcttccgcc
      181 ggagtgggcg ccatcggaca tttagttgaa acggcctttt ctatccaacg ctcagttact
      241 gtttggagca tcaatcttat gatgctgctt ctagccgctg tcctattagg aaaatcagta
      301 ttttttaaga ctgtagcggg aagtctgctt tttcctgttg ttttagagat tgttccaaaa
      361 gtggaaatga tctcctcaca tttcttttca ctgatttcag gaagtctttt gttcagtctt
      421 ggggtttaca cattgtacac agtcggggcc tcaaatggcg gtataacgat accgccgatt
      481 attttccaca aattttttcg gcttccaatg ccgcaaggac ttttacttac caatagtctt
      541 atcgtctttt tgaattatta tgtttttgga ttgttggaga cactattcgt tctcctatct
      601 attacagtca gttcattgtt tatgaaaata ctcatccgct tgtcccctgt gtcaagaaaa
      661 tttgaataaa gctggatttt gtcatagcgg aaacaaacga tatattaaaa agagtgtgct
      721 ataataaacc aagaaattga ataaagcgag ggatcaatca atgagcttct ctttagaaga
      781 catccgtcat ttactattaa aagaaaatct attaaaagaa tttgtttcaa caagaggctg
      841 gcatcttgat gtaccagaaa aagctgaatt tcttcagtta agttatgatt caagaaaagc
      901 tgatgcttct actttgtttt tctgtaaagg aatgaatttc aaagaagaat atttggattc
      961 agcaatcgaa cagggtattc aatattatgt atcagaacaa ccatacgaaa attcagcagt
     1021 aggaatcatc gtaacggata tccgtaaagc aatggcgctt cttgctatgg ctttttacga
     1081 ctatccgcaa aataaattga aagtgatcgg attcactgga acaaaaggta aaacaactgc
     1141 tgcgtacttt acaaaagcaa tcttggatca cacaacaaat aaaaaaacag cattgctttc
     1201 tactatgaat acaacgttag atggcaaaac atatttcaaa tcccatttga caacgcctga
     1261 gtcattagat ttatatcgta tgatgtcaga agcagttgaa aatggtatga ctcatctagt
     1321 gatggaagtt tcttcacaag cttacaaaac acaacgcgta tacggcttga ctttagatgt
     1381 cggaatcttc ttgaatatct cgcctgacca tatcagtccg atcgaacatc cgacatttga
     1441 tgactatttc tattgcaagc gacaattgat cttgaattca aaaacagttg ttttaaatca
     1501 tgaaagtgac tattttgatt tacttaggga aacagccgat ctattccaga ttccaacgat
     1561 cacttatggt cgttcggaag aaagcaatta tcaagtgatc cgttcagata aaggtactca
     1621 cggttttact ttatcttctc atgaagatcg cttagctatc tgcgatactg cttatgatat
     1681 tttgctggct ggaggattca accaagaaaa tgcagccagt gcgatcattg ccgcagcact
     1741 aacaggtgcc tccagtcaag atgcacaaga cggattaaaa gaagctcgtg taccaggccg
     1801 aatggatcaa ttgatccaat ccaatggagc gcatgtctat gtggactatg cacataatta
     1861 tctaagcttg aaaacattgc tgaaatttgc caaaaatgaa catccagatg gccgtgtaat
     1921 tgttgtatta ggcagcccag gaaacaaagc aatctcccgt cgacatgatt ttggaaaagt
     1981 gctatcagaa acagctgaca tcgccttttt gacggcagat gacccagcat ttgaagatcc
     2041 gaaaaaaatc gcggaagaaa tcaatgaagc aattactaat ccagatttga tcgttcatta
     2101 tgaaatggat cgtcctgaag cgattcgacg cgctttagca gaaagcgctc cgcaagattc
     2161 agtcgttatt gcgggaaaag gcgtggatcc ctatcaaaaa atcaatggtg tagatgaacc
     2221 ttatgaaggg gattatgcaa tcgtgaagcg gttgattgaa gaataatggt atttatatac
     2281 tagtggtttc ccatttctaa gaaaaaaagg gactgtgatt ttttgtcaca gttccttttt
     2341 tcttagaaat cattttaact tttcactagc taattctgct gccttttctc tttcttcatt
     2401 taataattta ttatcataga atttgataaa tgggaaccag ataatgaatg cgatgattgc
     2461 acaaatgact gctaacacag cagctttcca atcaccacca ctaccaacaa atgctccgat
     2521 cccgactggt gaaggccacg gcatctgtgc aagcatcggt ttgacgattt ccagccgaat
     2581 ggcaaaataa gcgagtgtag cagaagccat cggagctaag aaaaacggaa ccgctaaata
     2641 gggattgtag acgattggca tcccgaaaat gattggttca ttgatattga ataatcctgg
     2701 tacaagtgag gcttttccta acactttcag ctgatcagat ttcgcgagga atgcaataaa
     2761 gatgatcaat cctaaagtcg cacctgatcc accaacggtt acataagaat tgttgaattc
     2821 tcctgccaac ggataatttg cgccttgtgc gttagctgcc atattagcta atacgatcgg
     2881 ggtcaagaaa gaagtgatga tattcgctcc atggatccct acgatccaca atgcatggat
     2941 caagaaataa atcaccatga caccaagcca tgtattcgtc aactgcgtaa caaacccaaa
     3001 tgggatagca atcattttga agatatccgt tcctagtgca actaataatc cattgatgat
     3061 catcaccaca aacgcaatga caaatgttgg gatcagagca gtgaaagaac gagaaactcc
     3121 tggcggaaca acatctggca tttttatgac ccaatttctc ttgacacaca tacaatacaa
     3181 ttcgaccgca ataatcgaca taataatagc tgtaaagatt cctgaagttc ctaaacgttc
     3241 gacaaaagag cccatcctaa taccatttac tacagtttct gtctcattga tagaagcaat
     3301 caagctgatt ttaccatcag tcatgatcaa ttcagggata cacataaaga atgcaaaaac
     3361 agatagtaat gcccccgtga gcggattaac atttaatttt tgttcatctc gttcaattga
     3421 cgttaattca taagcaaaca caatggcaaa gtaaatagat aaaatcccca tcgtcattgt
     3481 attgacgatc atgtacaagt cactaacttt gaaaaaagtt gcattaaaaa atccttctaa
     3541 aaatgggaac ggtaatggca aaatatttaa aaccaaaaac atcgagccta caatcgtgaa
     3601 aggaatagaa gccatccccg ctgccatgac agcccgaacg attttaagtt gcgcaatttt
     3661 ccccataggt cccatcaggt atttctgcaa aaaatcaaac attagttatt ctcctttcct
     3721 aaatataacg ctttttcata ttgtttaata cggatatact gttggtcttg gttgagtgaa
     3781 attttcttta aacgatactg tataaatgca ctggcaaaaa taccgcttac aacaagaatg
     3841 aaaacaaata actgtcctcc attgttattt gccataaaag gatacaagaa tgtaaatcct
     3901 gaggtaaaca acactggaat cactaataaa ttaaagacac tctggaacaa aaaataccac
     3961 tttgtccatg gaacgttcgt ttggtgtttt cgataaagct ttacttgttc tcctactgca
     4021 ggcaaaatag ctaaaatcag aatcatcggt aaactacttc ctaaactttt agctgataga
     4081 agcagaatca tccattgcat gttcatgaaa aagaaaaagg cagtcaggta acgtacgatt
     4141 agataacgat tgaaatacat gtttttcaaa ctaagttgct ttttttctaa ttcgattttc
     4201 ctttttactg tatcctgcac aatgatcctt cctttctatt tatttttttg atacaaataa
     4261 ttcatttcct gtgcaacttc caataatgtc atcgtcgtca tcagatgatc ttgtgcatgg
     4321 accatgataa tttccatttc tatttttgtc ccgctggcat attctttcag aagttgagtt
     4381 tgtgactgat gcgcatgaag aatctcttca tttgcttgtc gcaacttctc ttctacttct
     4441 tcaaacatct ctttacgcat cgagcgaaat gcttcatgta tcagtgtccg tgcatttccg
     4501 ctatggaaaa tgatatcaaa agaagtcact tgtagtgttt cactgtttat tttttcagcc
     4561 atctgttttc ctccttattc gatcagtttg ataaatttac tgctgaaatt ctcaaaaata
     4621 ggttcagcta agatttcctg ctgtactttt agctggtcaa ctaacctaac gattgcttta
     4681 gtcacgaccg taattccttc attttcgaca taagaaggcg aaactaaaaa aacaaaatga
     4741 atttcttgat attgatccca ctgcatccca gaaggaatca atgcaacggc tacctttgtt
     4801 tcgattccaa ctggaacagc gggatgtggg actgccactg tatcactgaa tatgatttgt
     4861 cccatatttt cacgttgctg gatctgcttt cgcatttctc ttatatatcc ttcttcttca
     4921 tttatgctca gcttttgcag caaatcatga agaatttctt cctttttcgg ttcgtacgta
     4981 taagtcttga aaaatttttc agacaactgt tcggtgtaaa tctgcttttt ttgctgtaga
     5041 gaaaatgctg gtatcggtct atctgaggaa tgatagggta tagattctcc aactaccttt
     5101 cgtattttac gaacatcatc ttcattcaaa aaaatactga catgtaaaac tggaattttg
     5161 aaaaacattg tcgaaagatc aatagaagaa ataatcaggt cgattccttt taaagttgtt
     5221 tcattgatct cataatagcc ttttacactt accactgtaa tatttttacc gaattcactg
     5281 actacgcgat tctttaatag ttgggcactg ccgtaacccg ttgcacagat aatcaatgca
     5341 tgtagtttat gcgcagctct ttctttttcc aatgccgcca tgagatgcaa agccaaatag
     5401 gcccattcat cttcattgat ggtatagttt ttcaaagccg gcatgttgct gaaatgcctt
     5461 tttactgcgt caaagacttt gggattttct ttctttatct cctcagtcaa tgggttctca
     5521 atgcgaatac ctcggctcaa tcgaatcatc atcggtgaca aatgatcgag tagtccattg
     5581 aaaagctgga gatcctctac taatgaaaaa tgaaattcct gagacaatct tttcagaact
     5641 ttcgctaatt cctttgatag ttcctgatca ttttgatttt ctttgtgatt cgacttagcc
     5701 atgagatgta atgtcaaata agcaatttct tcttcgggaa acgatacacc tgcgattacc
     5761 tcaatacgtt gaacgatccg ctgcgccact tgatactcta ctcgcttaga aacttcttgt
     5821 tcgatgccta attccttgat ttcaaaccct tctcggagcc gtttgatccc caaagccaag
     5881 tgtaaagtca gattctgtat gataaaatcc gaaactttta acttagcttc tcttgtttca
     5941 tcgagaatga taatgactag ctcttcaaaa ctgatgtctg ggaaaaaacg gctttgccct
     6001 aaatattctt tcaatgaatt tccataatgg tttccaaaaa aagtatccat gataaaatgc
     6061 cgtcgatttc tttcttcccc ttcgatccat gtcccttgac catgcttaga aacgattttt
     6121 agtgaatatg gaatcaatcg ctccttgatt tcttgcatgt cctttgtcaa actggaacga
     6181 ctgatataaa gctcttctgc caaatcatcc ataaaaagaa tggcatcttc caaaagcaat
     6241 tggttcaata tatacttttg tcgatcttcg gactcataaa atccttgcgg ttctctagca
     6301 tatccaggta ctatttcttg ccgactcaaa aacaagtcaa atgtcagtct ttgtaaaata
     6361 tgcagttggt agccataacc tggtttagca atgatcttac ctccgttttt ttctactaac
     6421 tctttgatcc gctgcaagta attacggatt gtccgatcag ataataacaa ttcagaagct
     6481 aaacgttgac tggtgacata ctccttcttg tgatcaatca aatatctcaa cagcaatttt
     6541 tccttctgtt tcattatcat ccactcctat tccatcatat gttctcttcg atcacctctg
     6601 ctaacttctg gatgcctgta gggattggga catacgcttg gaaagggata ctaacaacag
     6661 gctttccaac agattctcct aattttttga atttatccaa aaacatcgtc gtttgcgggc
     6721 tgatcagaaa taaatcaaaa tcactattct taatcatatt atcgccttct gtagctgaaa
     6781 ctgcatctac gagtacctct tctctttttt gcttaaagaa atccgttgtc tttgaggcca
     6841 tcatcgagga tgacattcct gccgcacaaa taatcaatgc ttttttcata aatcaatcac
     6901 tccttttatt tttataaacc aagcatatct tgtaagcgtt ttctattcaa aatatatatt
     6961 tccgtagcat tgcggaaaaa atgtgtatca tatcttgaat aataatttct ggcaccgtga
     7021 tatactagtg atagaatttt ttcaggaggg gatttagtga gtatcacagt gaatattttg
     7081 tatacaagta caaatggaca agccagaaaa tttgcagaag aaatggtcga aagaaacatt
     7141 gtcaaagcga ttcgtgcaga agaaggaaac gagaaatatg actattactt tccactagaa
     7201 gatccagaat ctcttttact gatcgaccgt tggaaagatg aagaagcaat cgaaaagcat
     7261 cacaaatccg aaatgatggc tcaaataaac gaactccgta aaaagtataa attaaaaatg
     7321 aaagtcgaac gtttcactga tttaccgtaa aaagatacac atggaatgat agcaatttat
     7381 catccatgtg tatcttcact ttttccttat tttttattcg atctcatcta atgattcaaa
     7441 ccgtgttttt ctcttatttt tcggataagt atttcagtca ccccaatata catgattcct
     7501 aaacataccc caaggatatt ctcaaaaatg cggatcgtta ctgctcctgg aaccccgaat
     7561 aaactggcag tgattgccaa agcaccaaaa gaattaaaaa ctgttttata tctataggta
     7621 gaacaaacac ctaaagctag accacctaat ataccgaccc agttaaaagg aataaattgg
     7681 ctgataccaa taaataaaac acatccaatc aatgagccaa tgattcggtc tactgctcgt
     7741 tctttgaaac caatcgacat caatccatag ctagatacaa tagaagaaaa tgcaaaccct
     7801 gcccacatca atcgctgaaa agggacatac tctcctgcaa cgatcaaaag actaatgcca
     7861 aatgcataga tgagcatcca aattttttcc tgcgaataca tgcctttcaa aaaaatctct
     7921 ttaaacaagc ttttgtcttt gttcttttct cgatgttttc tgtaaaggag aactgaaaac
     7981 caacaaaaaa acaaaaataa taagctactt gtttgtgtaa agaaattctt gttcaacgaa
     8041 tctttaggta acgaataaac aataaagaga tatgaaaagc cgtacaaaga agcattcccc
     8101 attttgggat cactagcggt tgccattaat atgctgctta gtaaaataaa atgaatcaac
     8161 atgcccataa ctgaccattt gatcagctgg acatacggtg ctactaatag tgagaacatg
     8221 acgattgcta aacctatgat cgaatgggaa atcttataac caaagtcaac aaaacgaata
     8281 ctcatcaaca tacagaataa aaccacagcg tatggtttat gcttcgattc aaataatgtt
     8341 gtaattataa tgataaaaca aatggcaaac aatacgatga ataatgatcg taaaaacaat
     8401 gaacaaatat acatgcactt ttctttttta gaagctgctt ctcgtatttt ttgttttaaa
     8461 ataaacggat ctagttgtag agactgataa aaatccacct gttgtcgtcc tttcttttta
     8521 cttttatttt ttacagtcat ctatgacagg ttgtaaatac tatggaacat catatggcgt
     8581 tgtcattttc ttgtcaacta tttttcctat tcatgttttt ataaaaaaac atatcgtttt
     8641 cttgtatga
//
LOCUS       DACENJ010000111         8449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.114, whole genome shotgun sequence.
ACCESSION   DACENJ010000111 DACENJ010000000
VERSION     DACENJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.114"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            87..695
                     /locus_tag="IW748_001796"
     CDS             87..695
                     /locus_tag="IW748_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1104705.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            863..2500
                     /locus_tag="IW748_001797"
     CDS             863..2500
                     /locus_tag="IW748_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1104706.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2572..3684)
                     /locus_tag="IW748_001798"
     CDS             complement(2572..3684)
                     /locus_tag="IW748_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1104707.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(3759..5132)
                     /locus_tag="IW748_001799"
     CDS             complement(3759..5132)
                     /locus_tag="IW748_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1104708.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5353..5970
                     /locus_tag="IW748_001800"
     CDS             5353..5970
                     /locus_tag="IW748_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104709.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6068..6523
                     /gene="argR"
                     /locus_tag="IW748_001801"
     CDS             6068..6523
                     /gene="argR"
                     /locus_tag="IW748_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1104710.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            6541..8043
                     /locus_tag="IW748_001802"
     CDS             6541..8043
                     /locus_tag="IW748_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1104711.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            8138..8344
                     /locus_tag="IW748_001803"
     CDS             8138..8344
                     /locus_tag="IW748_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104712.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
BASE COUNT         2905 a         1579 c         1746 g         2219 t
ORIGIN      
        1 atgtttcttt tacctatgaa atatttctaa atgataaagt taatagcgtc gaaagaataa
       61 aaaacggaga aaaaaaggag tttaatatga attcatttaa gaaaattgtt ttaggtacaa
      121 cttttgctgc tggtgctact gcaatgtttg taggaactac aaatgctcac gctgatgaag
      181 tttatacagt aaaatcaggt gattcattat ctaagatctc acaaaaattt gcaggagaca
      241 actcaatgat cgatgcaatt gctgaaaaaa attcgatcgc aaacatcaac cgcatttatg
      301 ttggtgaaca attaacaatc ccaacttcaa atgattcttc agcaacaaca gaaaacaaaa
      361 caacagaaaa tacagcatct acaactgaaa cagcaacgca agaacataca tacgttgcac
      421 ctgttgaaac agttgaagta gctcctgctg ccccagctgc tgcaacagcg ccaacttcaa
      481 gctctgcaaa agaatggatc gcacaaaaag aatcaggcgg ttcatacaca gctactaacg
      541 gacgttacat cggccgttat caattagatg cttcatactt aaacggcgac tattcagctg
      601 ccaatcaaga acgtgtagca gaacaatatg taacatctcg ttatggttca tgggatgctg
      661 ctaaaacatt ctggttagca aacggctggt actaagccga taaaaggaaa atgatgaaac
      721 cgagaaactt gtttattcaa gttctcggtt tttttgtgtg aaaaataact cgtttgcttc
      781 ccacagcgca gcagtacaaa taaaaactcg aataatgtat aataaagaaa acgttttttt
      841 gtttagggaa tgaggaggag ccatgagtac aaaagagagt ctcgaattaa acgaaatcac
      901 aagtggaaat ccactgttat ccagtattcg atcgattgta gaaacgacat tttacggtaa
      961 taatgttcat gaagtttttg ataggaagac agcttatcaa ctagcaaaag ggagtccagg
     1021 aacgatcatc acagatttaa caatctcaca tgctgaagaa ctagatttac cagctgatgt
     1081 taggacacta gtattcaatg atggaagcat tgtgggaaga acagcaagtg ctcggcggat
     1141 ctttgaggac ttagacaagg aacaaagcaa atacgagaaa attttaagag aagctgtgta
     1201 tcaaagtcgc aagcgccagt tttaccatac aaaagtaatc gttggcctat ctgaagaatt
     1261 ttctgtgcaa agtcatttgc tgattccaga aggatttgag aacaatttgt attcttattt
     1321 gctgaatttt cagccagcga attcgaagat cttagagact tatcaaaagt ctgtttcctt
     1381 tgatgaggga gatctttata tcttttgtga tcccgaatgg acacatgaaa catttccaaa
     1441 tggactcatt ttgtttgatg ctctccataa tacagcggtt gtgctaggat tacgttactt
     1501 tggagaatta aaaaaagcga ctttgacgct tgcttgggca ttgggaaatc gcaatggctt
     1561 tattgcctgc cacggtggga tgaaacaata tgctagtaat gaccagacct atacgatggc
     1621 tgtatttggg ttgtccggtt ctggaaaatc aacgattacc ttagcaaaac atacggaagg
     1681 tcactctgtt tcgatcttgc atgacgatgc tttcgttatt tctcgaaaaa atggttttgc
     1741 tactgccttg gaaccttctt actttgataa aacacaagac tatccgtctg atgatccggc
     1801 catttcttct ttccttactt gtcagaatgt gggcgtgaca tgtacaagta aaggagaaaa
     1861 agtcctagta acaggagata tacgaaacgg aaatgggcgg acagtcaaat cacgttatgc
     1921 gacacccgac cgagtcgatc atcttagcga aaagatcgat gctgtttttt ggatcatgaa
     1981 agatgattcg cttccgccaa ttatcaagat caatgaccca gttttagcag cgtcttttgg
     2041 tgcaacatta gcaacaaaac gatcagacgc ggaaaatatc actacaaaaa gacagctaga
     2101 tgagctagtc attgaaccat ttgctaatcc tttccgtgtc tatcctctta gggaagacta
     2161 tcaagctttt cgagagttgt ttttgaaaca gcaaacagcc tgctatgtcc tgaatactgg
     2221 atatttcgat gataaaaaaa tcaaacctga aacgacattg tcaatcgttg aaagaatcgt
     2281 gcaacaaacg gcagaattca agtcgtttgg tccatttgaa gacatccact atttacctat
     2341 accagatttt cctgtttctt ttgaggatac gtcctataaa aatctggtga aacaacgact
     2401 tgatattcgt ttacgattca ttgaagctca gataggaaaa gatctcttgc cagatgaagc
     2461 aaaagaaagt ttggagaatt tgattcagaa attgaactaa aataacagct ttttcaagaa
     2521 gtaatccatg cgcaatatcg ctcgggatcc ttacttgaaa aagctgtttt tctatgatgg
     2581 atttttcgta atataggtgg agatgtcctt ttgataatct tccgtaggcc aatgaccagt
     2641 atttaagaag ttggctaata gatagccgat ataaggacct gtggtcaagc cagaagagcc
     2701 tagaccgcta gcagccagta agcttggatc atctggcagt acgccgaaaa atggggaaaa
     2761 atctgacgta taagcacggg tacctacacg gaactcgtat ggccagtttt ttatctgatc
     2821 tggatcagtc aagaactgtt ctgtactctt agtcaatccc ttataagctt cgctagtagg
     2881 agccaaatcc cagccttgat cgttttcatg ggtagcacct aaaaggatct ttccatccat
     2941 gaaaggaatg aaatcagctt ctccgtcaag catagcaaca ggccagtctt gactcatctt
     3001 ataagatgtc tgaaatgcta agagctgccc tttttgcgga cgtatatctg cagcatagcc
     3061 taattcttgt aataaaggct taagtgccgg gccaggtgtc aaaattacac gatcagctgt
     3121 gtattctcca aacggtccct tgatgagcca agaatcgctc ttcttatgca aagtgacttt
     3181 tgcctggatc tgctccattt gtttttgtgc taaacgctga ccaagatgtt ctaaatatgc
     3241 ttttccatcc agacgaccac caccactgat acgcagtgaa actgctggtt tcaataacgg
     3301 aagtgcttga gatgtctgtt caggtgaaag ccattcgatg tttccaattt ctggcgcttc
     3361 ttcttttcgt ttctcggcaa gaacagccaa ttcagctaaa tctttttcag gtcgaagcaa
     3421 aagtgtccct gattgtgcgt aaatcgtttg atcaagttgg aaatctttta taagtttagg
     3481 gaaaaaagca gcgccgtctt tagccagacg ataccattgt ttgtttctgc gtttcgacaa
     3541 ccaaggggag ataatcccag cgctagcttt agttgcctgt cctgtccctt catcaaataa
     3601 agcaacattg aattttgatg tatctaagta actggctagg gtcataccaa tgatcccgcc
     3661 accgataatg gcaatttttt ccataaaaaa gcctcctttt acaaacaacc attttcacac
     3721 aaaaaaacgg cagatgcaac acctgccgta tatggaatct acgctttttt agcaaagaac
     3781 agataatagg cgaatcgagc aaagcttacg agaagcaaca gcaacaagac gcctaatgct
     3841 gcttttgatc ctaatgtagt agacacttca tagttatggg aagtatgatg ctggacgtaa
     3901 ttgataatga ccgctactaa tgaagtagcc acagcgccag aaaactgctg caaggtattg
     3961 aacattgcgt tcccgtctcc ttgtagttct tcattcagtg cattcattcc ggtggtcatc
     4021 atattgctgt aagataatcc gataccgatc atataaaaca catgacaagc aatcaataca
     4081 cccactgaag cgtgtcctat gagtaggaat aatgctagcc aaccaatcaa agaaagaatc
     4141 agcccagcaa aaattggctt cttcggacca tattgatcca gcattttccc ggaaaaagga
     4201 gaaagcaaag caccaaccgc tgcgccggga agcatcgcta atccagcgac aaaaggacta
     4261 cttccttgaa cgatctgaag gaaattcggc aagacaaaag atacaccaag taataaaaac
     4321 tgataaacta agaagccaat caaaaacaag cggaatgttt tatttgaaag aatagacagt
     4381 cggatcagtg gatgctccac ttttttactt cgatagtaaa aaaggaacca gccaataccg
     4441 ccgatgatta aagcaatcca gctggaaact gttcccaacg tactcaaaaa tgttagactt
     4501 ccactaaaca aaagacaaat tcctaaaacg cttatccaat ccaaataacc acttttttgt
     4561 actgccatct cgggaatagc aaaccagccg ataatcaaag aaagaatcaa aacgggcaca
     4621 agaaacagga agatatgatt ccatgtaaaa tgtgaagtca taatgcctcc ataagtgggg
     4681 ccaattgctg gagcaatggc cgttgtcaaa gtaccgacac ccatcatcgc gcctcgtcga
     4741 ttgagggggg caaatgtcaa gataatatgg aacatcaacg gaagagcgat acctgtcgct
     4801 gcaccttgga gcaatcgacc aaacaatagg accatgaagg taggagagaa gaaatcgatc
     4861 attatacctg ccaagaaaag cagatttgaa gctaaaaaga gttttttgag actaaaattg
     4921 cgattcaaat aagtagacag cggaacaatg atcgaaatca tcaaaagata aatcgttgtt
     4981 acccattgga cttgtgcagt cgttaaatca aactgtctga taagagttgg gaaagttaca
     5041 ttcatggccg tctcgatcaa tacgcccgca aaagacatca tccctgttgc aattactgca
     5101 ggtactaaaa gcagctgttt ttgttttgtc atttacatca cctctaaaaa atttcgtaaa
     5161 aaaagagagt gatttctatt ataaaaatag aaaccgcctc tctctcgaca cgccgaaacg
     5221 tgttatctgt acgatttatt tacttcactt attctagcac aaactgaaaa atagtgtcaa
     5281 cacgattagc caagtcactc aaaaagaggt atgataataa ataactaaat ggaatttcgc
     5341 ggaggaagaa aaatggaaaa aattgaatgg cgtaaagaca agagctatag tatgaaaaaa
     5401 agcccgcaaa tactagaggt tcctaaaaaa tcattcttta gcatcagagg gatcggcgac
     5461 ccgaatcagg aagactttaa acgaagagta gcctgtctat atgcagtcag ctatgcaata
     5521 cgcatgtcgc ctaaaaaaaa ttggctgatt cctgattatc agatgtatac tgtctatcca
     5581 ctggaaggac aatggggact gcaagaaaag tatttgcatg aaccagtgat gaaaaaagaa
     5641 catttttctt atcaattgat gatccgacag cctgactttg ttacagaagc gatagcacaa
     5701 gaagccattc agcgttctag caaacttccg gaagacttga aagaacaagt agtattcaaa
     5761 aagatggaag aaggtctttg tgcccaaatc ttacacgtcg ggccctatgc cgaagaacca
     5821 gcaacgtttg agaaattaga agcattcatc gcaaaagaag gctatgaaag agcttctaaa
     5881 gaacacaaag agatttacat cagcgatcca agaaaatcag cacctgaaaa aatgaagacg
     5941 attttgcgtg ttaaaataga aaaaggttag caacctgaat gaaaacaaaa aataaaagta
     6001 aaaaatacca aaatatgaag aaatattact tatcttttgc tataatcaac attgagaggt
     6061 gtctaatatg aaaaagaaac agcgacaagc actgatccgc cagatcatta cagaacaacc
     6121 tattgggaca caggaagagc tgcttgcgcg gctgcatgaa gcaggcgccg atgtgactca
     6181 agcaacgatt tctcgagaca ttcgagaaat gaagttagta aaaagtcaaa atgaaaacaa
     6241 aatagtacgc tatacattat ttaaccagcc atctgtttct ttaaatgaag aacgtttgcg
     6301 tacagcaatt agacgcgagg ttttgcgtat ccagtctgtt cagttcatgg ttatcgtatt
     6361 gactgaacga aatggtgccg atgtggtaac gaattggcta gatgaagtgg cttaccctga
     6421 agttgtcgga acgatggccg gtgtagatac attcattatt atttgccgct cagaagaaga
     6481 agcacagcga tttgcagaaa agctggaaaa aatgagggaa taaatgagga ggacttttat
     6541 atgaaagata tgaaagcgct gcaaaatgga tcggatattc gaggaatcgc attaccagtt
     6601 gaaggatatg aagtaaactt gacgacagac atggtaaaaa agatcggctg gggactagca
     6661 aactggataa agaaaaagaa gaaatcaacc gatccaaata aaaaatggac gattgggatt
     6721 ggtcatgaca gtaggataag cggaccagca ctgaaagagg cgttgatcca aagtctgaaa
     6781 gaacaaggga tacatgtcat cgatttcgaa ttagctacta cgcctgctat gtttatgtcc
     6841 actcaatttg aacagttctc gtgcgatgct gcggttatgc tgactgctag ccatcttccc
     6901 tattattata atggaataaa attctttacc tcagaaggag gagcagaaaa agaagatatt
     6961 gcttttatcc tatcacagca gcaaaaaata aaaagcgaac aaactggaag cgtagaaaaa
     7021 gcagaccttt tgacacctta tgcgcaagat ttagtcacaa aaattaaaaa ggggattgga
     7081 acactagaaa gcaaaccact aaatggttgg aaaatcattg ttgatgccgg aaatggtgct
     7141 ggtggattct ttgctgaaaa agtattgaat agcttaggtg cggattcaac aggttcccaa
     7201 tttttggaac cagatggccg atttcctaat catatcccaa atcctgataa taaagaagcg
     7261 atggaaagta tcaagcaggc tgttttagca aatgaagcag atcttgggat cattttcgat
     7321 acagatgtgg atcgttctgc agttgtcagt gcttctggaa cagtgatcaa tcgaaataat
     7381 ttgatcgcag tattaagtac aatcgtatta aaagagcatc caggaacaag aatcgtgacg
     7441 aattccccta cttcttctca tctacaaaga ttcattgaag aaaaaggagg catccaagtt
     7501 cgttatattt ccggctatcg aaatgtaatc aataaagcat tagaacttaa ccgtcttggg
     7561 aaagatacac agctagccat cgaaacaagc ggacacgctg cattcaagga aaactatttt
     7621 ctggatgatg gtgcatatgt catcgcaaag atcctgatgc ttttgccaga attaaaaaga
     7681 aaaggtatca cattagatga acaaatcaaa gagttgaaac agcccgtaga agtacaagaa
     7741 atccgattaa aattgttcag cgatgaacca cgcgaacaag gaaacaagat attagaagaa
     7801 atgaagcgac tgttggaaga agaaaaaggg atgaacttcg acccggaaaa tgaagaaggt
     7861 attcgatatg atctagaaga gccatacgga aacggctggt ttttgatccg tttaagctta
     7921 catgagccgt tactagtatt acagatagaa aatgatgaat tcggaaagaa caagttggcg
     7981 ttagtaaaat tagcaaaatt attcggacat ttttctgatc ttgaatcatt aacagcagaa
     8041 taaaccagtc tttttgaaag gctagttgct tggacggcaa aaaaagtggt atggttaaat
     8101 cgagtgctgg tagggagacc agcaaaagga ggaaaaaatg gtaacgaata tggttatttt
     8161 tttaacagta ttggtttatg gggtcaactt tctggtatat atgtatctta aaatcaatca
     8221 tataaaagat tatgtagaaa agattgctat ttattttgga acaaatatga cgctgttgtt
     8281 cacgagtagt atttttttgt tttttggaaa aatagtagaa gacggcattc tggtattcga
     8341 ataagcagaa tttttgtaga aagcgcctta cttgtcatac actataatta tagccaagta
     8401 gcaaatggtc aatcgaaagg agcagtacaa tgaaaaaagc atacgaaac
//
LOCUS       DACENJ010000112         8405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.115, whole genome shotgun sequence.
ACCESSION   DACENJ010000112 DACENJ010000000
VERSION     DACENJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.115"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            259..1011
                     /locus_tag="IW748_001804"
     CDS             259..1011
                     /locus_tag="IW748_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1104713.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1033..2187)
                     /locus_tag="IW748_001805"
     CDS             complement(1033..2187)
                     /locus_tag="IW748_001805"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1104714.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            2372..4813
                     /locus_tag="IW748_001806"
     CDS             2372..4813
                     /locus_tag="IW748_001806"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1104715.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(5143..5847)
                     /locus_tag="IW748_001807"
     CDS             complement(5143..5847)
                     /locus_tag="IW748_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104716.1"
                     /translation="MKKKQKQELLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(6034..6279)
                     /locus_tag="IW748_001808"
     CDS             complement(6034..6279)
                     /locus_tag="IW748_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104717.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(6296..7147)
                     /locus_tag="IW748_001809"
     CDS             complement(6296..7147)
                     /locus_tag="IW748_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1104718.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            7313..8317
                     /locus_tag="IW748_001810"
     CDS             7313..8317
                     /locus_tag="IW748_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1104719.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         2713 a         1475 c         1729 g         2488 t
ORIGIN      
        1 gagttggcag gagaacaccg tttaaggaag aagttgtaaa atcagcattt tgagctaata
       61 ctgagggtca tctgattgga cactgtttat tcggaaagag ggactttgac acgactttgg
      121 tcacagtccc tttttttatt tatgatagac ggaaagccaa tcattttatt attcatcaaa
      181 agagatagaa tagataaaag aggagaatga tttattccga aaagtgaaaa catataacaa
      241 aaaattagag gaaatcaaat gaatgaaaaa gaatgggatg aatttgctga agaatattat
      301 gaaatccagc aagaatcgca agtaactatt gctgaagatg taaaaaatta tctgaaaacg
      361 gagaaagtcc tgcctgctga aacagcagca gacgttgctg gaggatctgg cagatttctt
      421 tccttagcaa aagaaattaa tcgctatgat ttgatcgatt tttcttcagg aatgctagaa
      481 aatgcaaaaa aagaagcaga aaaaaatgag ttgccaaatg tttcctttat caaacagtcg
      541 ttagaagaat ttatccaatc aaatgagaca tatgaaatgg tttttacagc agcaaatcca
      601 gcattggtac atcttattca attagaaaaa ttgatgaaaa agtcaagcaa atggtgcgtg
      661 ataatcagag tagtggaatc aatcgatgat gttttttatc ctatcgaaaa gcaactagga
      721 atgatagaag aagatcctaa tacatcgcca gttgtaatgg atcaatttga aacatactta
      781 attgatgaag gattcaaagt gaagaaaaaa gattttactt attttacaca ggaatcaatt
      841 actgaagaat tgatgaagct atactatgac gcgtacgcaa aagatcctgt tgtgaccagg
      901 tacattcgca aactattcag tgaaaaacga gaaatcctta gtacgactaa gctgacctat
      961 agactgctcc tttggtccgc agataaaaaa agaacctttc acgactcgtg aagggttctt
     1021 tttatgattt tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa
     1081 aggtacatct gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat
     1141 cgtttcatat tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg
     1201 tgcgaagaga tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc
     1261 accagaaccg tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc
     1321 cagtagagaa gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct
     1381 gtaacggatg ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg
     1441 gtctaatacg ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat
     1501 cgcctgataa tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg
     1561 gaatgattcg atatacgtag aattagataa aggaccatca acaacaggaa attcgctata
     1621 atctggacgc cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga
     1681 taaaatacgc gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc
     1741 accaccgctt gccaagccgt aacgagcaat atcacttgct atgactagta cttttcgttc
     1801 tggatgattt ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc
     1861 ctcttttact tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac
     1921 cgcactcgct tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag
     1981 ctcacggtct ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc
     2041 ttcatttgca cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg
     2101 atcatcccca cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa
     2161 ggaaagacga tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta
     2221 ctttactaag gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt
     2281 gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa
     2341 gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc
     2401 aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg
     2461 cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca
     2521 agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc
     2581 tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc
     2641 tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt
     2701 gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga
     2761 aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt
     2821 ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag
     2881 agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc
     2941 cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga
     3001 ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt
     3061 ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt
     3121 ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat
     3181 caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc
     3241 cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat
     3301 caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga
     3361 aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc
     3421 acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc
     3481 ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca
     3541 aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag
     3601 tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac
     3661 actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc
     3721 aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac
     3781 agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg
     3841 cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta
     3901 tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga
     3961 aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt
     4021 catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat
     4081 gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt
     4141 ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc
     4201 tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat
     4261 tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac
     4321 gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac
     4381 acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt
     4441 aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt
     4501 tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt
     4561 agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa
     4621 cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca
     4681 agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa
     4741 aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat
     4801 ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac agttatttta
     4861 tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta gaatttttta
     4921 tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca ggtagaagag
     4981 aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc tgatatttga
     5041 gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat gactaaaaag
     5101 gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat cagtaatcgc
     5161 tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt atttccgttc
     5221 gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg ctaatttacg
     5281 aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt ctgtgtaaaa
     5341 tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg cattcgtatc
     5401 agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt cttctggcac
     5461 actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt catcgttttt
     5521 caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac ggaatcgttc
     5581 gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt tgtcatcgat
     5641 tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg ttaagatttg
     5701 acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca agctctcttc
     5761 ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga attgatttaa
     5821 taattcttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc ctttcgatta
     5881 taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta tacgcaaatt
     5941 acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa atggaacgga
     6001 ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca aggcaaaaac
     6061 aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca ttagcgaatg
     6121 atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc ttgcaggaaa
     6181 atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag tctgggtaac
     6241 ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt tcgacttaaa
     6301 attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga tcgatttgtt
     6361 tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg attctgcttg
     6421 gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc aaagcaactt
     6481 gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca tcgaagattt
     6541 taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt gcatactcaa
     6601 ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc ggcataattt
     6661 ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca ccgattgctt
     6721 tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca tttgcttgtt
     6781 cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga tctaaataat
     6841 ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca tagctgtggt
     6901 ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca ctttccttaa
     6961 tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata tggcgataac
     7021 ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt gtttgccatg
     7081 tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg cttgttaatg
     7141 aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc atttttcgaa
     7201 aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa tgattttgat
     7261 tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga aaatggatat
     7321 ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc cagtcttttc
     7381 acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa agaagatcca
     7441 aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca tgatcgttga
     7501 atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg ttcagcggtg
     7561 gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg ttgtcttgta
     7621 tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg aggcttacac
     7681 tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga atcgagcaat
     7741 tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga ctgattggac
     7801 ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt atttttctgg
     7861 aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag tgatccaaaa
     7921 agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca tggctgattt
     7981 gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg agatgattgc
     8041 ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag aagtacaaga
     8101 gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt ctgagaaaaa
     8161 atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac cagtagatgt
     8221 atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga tatccgtgat
     8281 gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat attaggtgtt
     8341 caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa agacgaatat
     8401 aacaa
//
LOCUS       DACENJ010000113         8355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.116, whole genome shotgun sequence.
ACCESSION   DACENJ010000113 DACENJ010000000
VERSION     DACENJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.116"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..282)
                     /locus_tag="IW748_001811"
     CDS             complement(<1..282)
                     /locus_tag="IW748_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1104720.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAI"
     gene            671..2437
                     /locus_tag="IW748_001812"
     CDS             671..2437
                     /locus_tag="IW748_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1104721.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2539..3798
                     /locus_tag="IW748_001813"
     CDS             2539..3798
                     /locus_tag="IW748_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1104722.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3945..5246
                     /locus_tag="IW748_001814"
     CDS             3945..5246
                     /locus_tag="IW748_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1104723.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            5249..6100
                     /locus_tag="IW748_001815"
     CDS             5249..6100
                     /locus_tag="IW748_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1104724.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            6113..6928
                     /locus_tag="IW748_001816"
     CDS             6113..6928
                     /locus_tag="IW748_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1104725.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            7079..8245
                     /locus_tag="IW748_001817"
     CDS             7079..8245
                     /locus_tag="IW748_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1104726.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2748 a         1447 c         1636 g         2524 t
ORIGIN      
        1 tattgcaatg attaaaggaa aaaccaagct tccgaaaagt actttaaaaa agggtttttt
       61 acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc
      121 tcggtcaaag cctaaagcag cttccagtaa tattcctaga ccgctgacac ctccagcagc
      181 aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat
      241 aatcttaatg tatgtccata taaattgtct cgtttctttc atttattcac ttccttaatt
      301 cattaataat actcttatct tattataact tacttttcac cttaagttaa ctttaattaa
      361 taaaaaaatt aggctagtct aaattattat gtttcattct tctagaatat acttctacgc
      421 caaaaactgt taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa
      481 ttcaggacac taaatagtac aggaagtaga ctaaaacttt taaaagtttt aagtctatct
      541 atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt
      601 gaaagcgttg tcttttgctt ctctatcact tatagtgatg acgtcatatt aaaacacaag
      661 gaggaaatca atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct
      721 atatacgaat acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt
      781 atatgtgcta tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg
      841 accaatgaaa aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag
      901 cgaaacaagt cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt
      961 ttacggtgat caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta
     1021 ttttcgaata ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga
     1081 aacagtatgg tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc
     1141 cgaacacact ttaccttggg gaagtaaaga tccagaccga gaagactttt tcgggggaga
     1201 tttgcaaggt gtgatggatc atttggatta cttgacggac cttggcatca atggcatcta
     1261 tttttgtccc atatttaaag ccacatccaa tcataaatac gatacgattg attattatga
     1321 ggtggatccc gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa
     1381 aagagggata cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca
     1441 gtggcaagac gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg
     1501 ttttttccca gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc
     1561 atatgatact tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt
     1621 gcaaaaatat ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg
     1681 gcgtcttgat gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt
     1741 tacagaaatc aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg
     1801 gctgcaaggg gatgaattcc atgcagtgat gaattatgca tttacagatt cgattaaaga
     1861 ttattttgca aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca
     1921 aatgctttac agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac
     1981 tgccaggatc ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt
     2041 tatgtttctt caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg
     2101 tgaagatgac ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga
     2161 gctattcggt tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc
     2221 tgaaggttct actcagtggc taatagtaaa tgatcgtgaa gataagctct atttcacacg
     2281 gaaactagag ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt
     2341 agaacaggaa aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc
     2401 agagatacga caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag
     2461 aaagcgtttg caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat
     2521 acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc
     2581 tctttaggaa tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg
     2641 gaagccgatg aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata
     2701 aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag
     2761 gacatgttcg atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac
     2821 gttatgatgg cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa
     2881 gtaaaactgg gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat
     2941 ggaaaaatct acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat
     3001 ttagtcgata cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt
     3061 tttgcttttg agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt
     3121 tattattcct atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca
     3181 aatccttcag atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca
     3241 gattggttca aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc
     3301 gccagccagc aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg
     3361 caaacataca aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat
     3421 ggacagcctt atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa
     3481 aacaaagacc tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa
     3541 ttctttgaaa tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct
     3601 aaaaacgatg aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca
     3661 aacatcccag aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca
     3721 tcaggttcaa aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc
     3781 atagaacaaa aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt
     3841 gcttcagata aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc
     3901 ttaaaagcag tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa
     3961 caagcaatat caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt
     4021 ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg
     4081 gtcagatgct tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac
     4141 caatggaaga tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa
     4201 tcattttctt gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc
     4261 gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt catacataat ataggtgatg
     4321 aaggttttcc ttatctattg acacttcccg cttatcttct catgattttt acgatcattt
     4381 tcccagtcct agttacattg ttcactgcat ttactaatta tgacttcaat catattccac
     4441 cagccagttt aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct
     4501 catatcgtaa tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa
     4561 caactttgca aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta
     4621 aattcaaacg tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta
     4681 ctatcatgag tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga
     4741 ttcctttcat aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc
     4801 cgaactggac gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct
     4861 atgtgatggt tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa
     4921 tcgacggagc aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag
     4981 tggcagcgcc aacatttatt acacagtata cggggaattt caataatttc tccatgatct
     5041 atctgtttaa caatggcggt cccggaagcg taggcggagg agctggttct actgatattc
     5101 ttatttcctg gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag
     5161 caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga
     5221 caaatgcgtt caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca
     5281 aaaacgactt tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat
     5341 ctatccgctt ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag
     5401 tttagatctt tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact
     5461 ttacgggact tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt
     5521 gactgtagta actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc
     5581 aagtttgatg ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt
     5641 ttatgtcatg gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta
     5701 tataggcggt ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc
     5761 aatagattta gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca
     5821 gattgtatta ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg
     5881 accttttggg gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt
     5941 tgctgtcggc ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc
     6001 tgctggggca attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa
     6061 ctttgttacc ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac
     6121 atttccgtta aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa
     6181 actattatca tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat
     6241 gcctgtgact cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc
     6301 cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga
     6361 aactgggtta caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt
     6421 gtctttatct gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca
     6481 atgggtgatc caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc
     6541 atctttccaa ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga
     6601 attttatgcc agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt
     6661 cctatttgtc atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag
     6721 aaaaagccaa ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt
     6781 tatgggaata ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt
     6841 gatgctaagt gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac
     6901 aagattcaaa gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt
     6961 gaaaaaaatg aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt
     7021 tgattctttt cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat
     7081 gttagagaaa actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt
     7141 aaattattgg gacggtacaa cgaaacaata cggagatacg gacactcctc cccaaatcaa
     7201 aatcaatatc catgaagaaa tcccaatgaa agagattaca agtaacgcat ctttggcact
     7261 aggagaagcc tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa
     7321 cgatgcttat cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc
     7381 aaagaaagaa aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt
     7441 aggtaacgac ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt
     7501 caatacacct gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga
     7561 caaactcttt atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt
     7621 gatgttcact gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga
     7681 acaatacgat ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt
     7741 attattaatg gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg
     7801 tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt
     7861 attgaaacca aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac
     7921 aaatgcttgg atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt
     7981 agttgataat attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga
     8041 ctaccaattg acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac
     8101 gaaagaaatg ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc
     8161 ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa
     8221 tgacatccca atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa
     8281 agaagctaag ttttatgatg ctccagaagt gaactttttt gtctaacttc taggattgtc
     8341 tctaaagctt agttt
//
LOCUS       DACENJ010000114         8336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.117, whole genome shotgun sequence.
ACCESSION   DACENJ010000114 DACENJ010000000
VERSION     DACENJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.117"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            264..1457
                     /locus_tag="IW748_001818"
     CDS             264..1457
                     /locus_tag="IW748_001818"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1104727.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1587..3059
                     /locus_tag="IW748_001819"
     CDS             1587..3059
                     /locus_tag="IW748_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1104728.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3142..3930
                     /locus_tag="IW748_001820"
     CDS             3142..3930
                     /locus_tag="IW748_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1104729.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3997..4665)
                     /locus_tag="IW748_001821"
     CDS             complement(3997..4665)
                     /locus_tag="IW748_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1104730.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4780..6492
                     /locus_tag="IW748_001822"
     CDS             4780..6492
                     /locus_tag="IW748_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104731.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6495..8267
                     /locus_tag="IW748_001823"
     CDS             6495..8267
                     /locus_tag="IW748_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104732.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2551 a         1391 c         1829 g         2565 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1501 ctttttttat ggcagttaac ctcttgaaaa agatagtaat tatttatcta tttttagatc
     1561 ctgtcataat gaaagggagg aataagatga aaaaagaaac aaatgttact ttggtcacag
     1621 tgagtatttt cattgcaacg ttcatgacgg ctattgaagg tacaatcgta acaaccgcta
     1681 tgccaacgat tgtcggttca ttacatggaa tggaaatcat gaactgggta ttttcgattt
     1741 atttgctgac aaacgctatg cttactccga tttacggaaa acttgcggat aaagtgggac
     1801 ggaaaccaat ctttattttt gggacattat tgtttattat cggttcgtta ttttgcggtt
     1861 tatctcaaag catgattggg ttgattgctg ctcgtgcgat tcaagggatg ggtgctgggg
     1921 caatgatgcc agtagctttg acgatcatcg gcgatcttta ttctattgaa aagcgagcaa
     1981 aagttttagg tttgaatagt tcagcctggg ggattgccag tgtttttgga ccgcttgcgg
     2041 gaggattcat cgttgaaacg atcagctggc actggatctt ttttatcaat gtgccgatcg
     2101 gaattctatt aattattttg atttggctat atttaaatga accaaaaatc atacatgagt
     2161 ctaaaccaat ggatattttt ggtagtttta gtttgatggc catgcttgtc tcattgctgt
     2221 tagggttcca attattagga gacgaaggaa tgagtccgct tgtttctgct ttgctgctag
     2281 gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa aagagctaaa gatcctgtca
     2341 tcatgatgca tttattcaaa aatccactat ttgttgtggt caacttggct gcagcattgg
     2401 ctagtggttt tttgatggga atcgatgttt atatccctat gtggatgcaa ggtgtattag
     2461 ggctgaatgc agcaattggt gggctggtat tagcaccatt atcactagtc tggatggtcg
     2521 gttctttcct tgcaagcaag tggatgactc aaatgaaaac tcattgggtc ttgagaattg
     2581 gactactaat cgcagctatt gggggcgctt ggttgtatct tatgcctttc agaagtaact
     2641 atttatggtt ttttgctata tcaacggtca tcggtatagg cttaggtgtg accatgacta
     2701 cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca gcttggtgta gcaacttctt
     2761 ttaatacgtt agttcgaaca attggtcaaa cagtcatggt ttctattttt ggtttattga
     2821 taaatggggt aactaaacga gaacttgcag cagcaaattt aacaaatgat gcagatatca
     2881 tgaataaact ggtgaatcca caaacggcta aatatattga tgcggatatt ttgttaccat
     2941 taagaaaaat tctttatgat gggttacatt ccgtttatct agttggcttg ttactcgtga
     3001 tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag tggtaaaata aaacagtagt
     3061 ttcggttaag atatgtacgt agccgaatca ttcaaattta aagttattca aaaataactt
     3121 cttactaagg aaggcgaaaa tatggcattt attcaagcaa atatttattc gaatgtgtta
     3181 gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa caaaaaaggt gattggcacc
     3241 agttcagagc aagagacaac ggatatacct gttttatatt tacttcatgg aatgggtggc
     3301 aatcatagtg tatgggaaag acgtacgtcg attgaacgtt atgtagcaaa ctacggatta
     3361 gctgtcatca tgccatctac tgatcttggc tggtacacag atacagttta tgacatgaag
     3421 tattggacat tcattgcaga agagctacct gagatttgtc atgaattatt cccgcaatta
     3481 acaagaaaaa gagaaaaaac atatgccgct ggattatcga tgggcggata tggcgcgtta
     3541 aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa tctctctttc aggcgctgtt
     3601 tctgtaggaa atcgaatgga tgatcttttg atggttcgta aagcatcttt ttgggaaggg
     3661 atctttggac cattggatca aatcgaaggt tcaaaaaatg atccgcaata cttattgaaa
     3721 gaactagtaa aaagcggaaa accagttcca agattttata tggcttgtgg ggaatctgat
     3781 tttctttatc aggcaaatca ggaaatggta tctgctatgc aggaacaagg cttggcggtc
     3841 acctttgaac aaggacctgg cgatcataac tggatcttct gggaccaatg gattcaacgt
     3901 gcattggcgt ggctaatgga agaaaaataa aaaaagagtc ttaaaaagat agctcctata
     3961 gagatcacaa acatagtgat ctctatagga gcttttttac tgctcgtttt ctcttctttt
     4021 attttgttgg cataagcctt caatataggt ttgtgcactc gttagaaaga gtgctttttc
     4081 attcaattct gcatgggact gcatactttc tttaaatccc tctaattgat tttcaactac
     4141 ataattacgc ataaatagaa ctgtttcttt caaaaattca tttccgtcca taattttttg
     4201 tcgaagctgc tgttcatgct gctgatccat taactgtcca atcaataaat tgtggagaga
     4261 ggcaatggcc tgatgacttt gggtgactga aaatccttca cgaacaagta attggatcat
     4321 ttgctccaaa tgttctaatc gactcggaaa agcgggcaca gtattgattt ctaattctgc
     4381 cccacaaggg aaacgtgtat aaaggtcata gtaattttcc atatactgca gcaactgttc
     4441 attccaaggc aaagttggat ctggaagcgc taattcttct tccattgttt cagccatttt
     4501 ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa gcaggtgctt gaacatgaat
     4561 tctgtttgcg atgtttcgca tggaaagctg cgaaagagct tgtgtttcag ccaggatatc
     4621 aaaggcggcc tgaatgatga ttgcttgaga aagctttatt tccattttat caacctcgtg
     4681 tattgcatta tctttttaaa catggtaaat tgttagtcgg tctaatttta cagtgttaaa
     4741 ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa tgaaattaat cttacgacat
     4801 gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt ccgtagcagt catggttgca
     4861 gcagcattat ggcaacctaa gttactacag caagtattgg aagctattat tacagaagat
     4921 aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc tggctttgct aggattagcc
     4981 gcaggtgtaa cgaacacgat tttctctgca aaagtagctc aaggcgtaag tgcagatatc
     5041 cgagaagaag catttcgaaa gatccagaca ttttcatttg gaaatatcga acagttctct
     5101 gctggaaatc ttgttgttcg tctaacaaat gatatcacgc aaatccaaaa tttagtgatg
     5161 atttctttgc aatcactttt tcgaattcca ttcttattta ttggtgcctt cattttagct
     5221 atgatcacga tgccgcaatt atggtggatc atcgtagcgc ttatcgttac ggtatttctg
     5281 attacggcac tttcatttac aaggatgggc aagcacttca tgatcattca aaaactgatt
     5341 gatcgtgtga atagcattgc aaaagaaaac ttactaggga ttcgtgtagt gaaatctttt
     5401 gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa gtgaggactt aacaaaacac
     5461 aacatcatag tcggtacatt attttctgtg atgatcccgt ccttcatgct ggctgccaat
     5521 ttggctgtgg ttggggcgat cttttttgtg agcgatcttg ttaaagatga tcctacgtta
     5581 attggtggaa ttgcttcatt catgaactac ttgatgcaga ttatgatggc aatcattatt
     5641 ggcggaatga tgatgatgat gacttcaaga gcagccgtat cgctgaagcg gattgcggaa
     5701 attttagatg ctgaaccaga tcttacttat ttagacgttc cagaacaaga gttgacgggt
     5761 tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg aggatacaga tacgcttaaa
     5821 gatatcagct tttctatcaa acctggagaa atggttggta tcgtcggggc gactggtgcc
     5881 gggaagtcaa cactagcaca attgatccct cgtctatttg atccgacaga aggcaaagtt
     5941 gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga gtttaagaca aacggtttcc
     6001 tttgttttgc aaaaagcaat tttattctca ggaacgattg ctcaaaactt gcgccaaggg
     6061 aaaaagaatg caacagaaaa agagatggaa catgcttctt ctattgctca agcaaaagaa
     6121 ttcatcgaaa agttatctga tcgttacgaa gcaccaatag aagaacgtag cagtaacttt
     6181 tctggtggtc aaaagcaacg tttatccatt actcgtggcg ttatcggtaa tccaaagatc
     6241 ttgatcctag atgacagtac gagtgcactg gatgctcgct ctgaaaagct ggtccgagaa
     6301 gcactcgatc gagatttgaa gggaacaacg acgattgtca tcgcacaaaa aattgcttct
     6361 gttgtcaaag cagaccgcat cttagtattg gatgaaggca gattagtcgg cgaaggaacc
     6421 catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa tctttgaaac tcaaaaagga
     6481 acggaggagt aagtatgact gatctaataa aagcaagtaa atttttctat cactatttga
     6541 agcggtacaa actctccttt ttctttatct ttatcacagt gatcattgct acctatctac
     6601 aagtaaaggc acctcaatac atcggggaag cttttcaaga attagctaat tacgtaggag
     6661 gcttgatgca aggcgtagac gataaaagca aatttgtttc tgtaatatgg aaactcttgc
     6721 tgttttatgt actagctagt gcagccaact ttatctatag tattttgttc acacaagtag
     6781 tagggaagtc aacgaaccga atgcgtatcg gtctattcaa caaattggaa aaattgacga
     6841 tccgtttctt tgattctcat caagacggtg aaattttaag tcgttttacc agtgacttag
     6901 ataatattca aaatagtttg aaccaagctt tgcttcaagt catcacgaat gctgttttac
     6961 ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga gttagcttgg gctacaattg
     7021 cttccactcc agtagccgtt ttgatagctg tcttgattat ccgcaaagca cggaaatatg
     7081 tggatcttca gcaagatgaa gtggggaaac taaatggtta tatggatgaa aaaatcagcg
     7141 gacaacgtgt catcatcaca aatggcttac aagaagaaac gattgaagga ttcttagagc
     7201 ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc ttattctggt ctactatttc
     7261 cgatgatgca aggaatgtca ctggtcaata cagcgatagt tatctttttc ggcggctggt
     7321 tagcattaaa tggtgatttg gaacgttcgg tggcattagg cttagttgtg acattcgtcc
     7381 agtattcaca acaatattac caaccactga tgcagatttc atctggatac agcatgatcc
     7441 aattagcgat tacaggggca agacgattga atgaaatatt tgatgaaaaa gatgaagtca
     7501 aaccagaaaa cggtaaagca tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag
     7561 attttggtta ttctccagaa aaactagtct tgaaagatgt ctcgatcaat gtggacaaag
     7621 gagaaatggt tgctcttgtt ggtccaacag gatcggggaa aacgacgatc atgaatctgc
     7681 taaaccgttt ttacgatgtc aatgatggtt cagtagtcat cgatggaata gatatccgag
     7741 attatgatct tgatgcatta cgtagacatg taggaatcgt tttgcaagaa tctgtattgt
     7801 tctccggaac gatcaaagac aatatcacat tcggcaaacc ggacgcttca gatgaagaag
     7861 tcatcaatgc agcaaaacaa gcgaatattc atgacttcat catgacgtta gaaaaaggtt
     7921 atgatacaga gatatccgaa gaaaataact tattcagtac aggtcaaaag cagttgatca
     7981 gtattgctcg aacgatcatc acaaatccat ctttattgat tttggatgaa gcaacaagta
     8041 atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa
     8101 gaaccagctt tgtgattgct caccggctga aaacaatctt gaatgcagat agaatcgttg
     8161 tcttgcgtga tggggaagtc attgaagaag gcagtcatca tgagttatta gcagaaaatg
     8221 gattttatgc ggaactttat cataatcaat ttgtatttga aagttaaatt aaaagaacct
     8281 cgttataacg gggttctttt tatatatttt atgttgtttg tttttataag gggcag
//
LOCUS       DACENJ010000115         8279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.118, whole genome shotgun sequence.
ACCESSION   DACENJ010000115 DACENJ010000000
VERSION     DACENJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.118"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            60..1583
                     /locus_tag="IW748_001824"
     CDS             60..1583
                     /locus_tag="IW748_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1104733.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            1820..3160
                     /locus_tag="IW748_001825"
     CDS             1820..3160
                     /locus_tag="IW748_001825"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1104734.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            3313..4050
                     /locus_tag="IW748_001826"
     CDS             3313..4050
                     /locus_tag="IW748_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1104735.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(4215..4436)
                     /locus_tag="IW748_001827"
     CDS             complement(4215..4436)
                     /locus_tag="IW748_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1104736.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            4467..5498
                     /locus_tag="IW748_001828"
     CDS             4467..5498
                     /locus_tag="IW748_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104737.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5485..6888
                     /locus_tag="IW748_001829"
     CDS             5485..6888
                     /locus_tag="IW748_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1104738.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            6954..7589
                     /locus_tag="IW748_001830"
     CDS             6954..7589
                     /locus_tag="IW748_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1104739.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            7638..>8279
                     /locus_tag="IW748_001831"
     CDS             7638..>8279
                     /locus_tag="IW748_001831"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1104740.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAI"
BASE COUNT         2724 a         1424 c         1779 g         2352 t
ORIGIN      
        1 gataaaataa cattagagcc aaaattgttg tctctaacgg aaaagaaagg aagaagaaga
       61 tggaacaaac aaaaaagaaa aaatttcaaa tgccttcagc gtatacggtc ttatttatta
      121 ttattgcagt aatcgctgta cttacttggt ttataccagc tgggcactat agtgtagacg
      181 atgcaggcaa catcgtcgct ggatcatatg aacgagtaca acaaaatcct caagggctgt
      241 gggatatttt catggcgcca atcaatggga tgctaggtgt accagcgggg gagctaacta
      301 aagaaacacc tgctgcaatc ccaatctctt tcttcatttt ggttgttggt ggatttttag
      361 gtattatcaa caaaacaggg gcactagatg ccgggattgc ttcagttgtg aagaaattca
      421 aaggaaaaga aaaaatgctt attccagtat tgatgctgtt atttgcatta ggcggctcta
      481 cttacggaat ggcagaagaa acgattccat tctatgcttt actgatccca gtcatgatgg
      541 ctgtaggctt tgatacagta gtagctgtag caatcgtatt agtagggtct caagtaggct
      601 gtctagcttc aacggtaaat ccttttgcaa caggcgtagc ttcacaagct gttgggattt
      661 cgatgggtga aggtattggt cttcgattct tgatgttcgt tattttgtta gctatttcta
      721 ttgtatatgt gtatcgttat gcatcaaaga tcgaaaaaga tccaacaaaa tcattagtat
      781 acaaccaacg taaagaagat atgaagcatt tcgatattga agcaatcggt cgtcaagaag
      841 taatcacgaa aaaacaaaaa catgttaatg tattgttctt tattactttt ggtatcatga
      901 tcatcagttt gattccttgg acaaccttga atgcgaattt cacgattttt gaatcattga
      961 caaactggtt aacaaacctt ccggtactag gtacggttct aggtaaaagc atgcttccat
     1021 taggtgactg gtacttccca gaaattacta tgttattctt agtaatgtcc atcgtagtag
     1081 caatcactta tggcatgaaa gagtctgaat ttgtctctga atttttagct ggtgcttctg
     1141 atttgatcgg tgtagcaatc gtagtagctg ttgctcgtgg tatccaagtg gttatgaaca
     1201 atggattaat cacagacacc atcttacatt ggggcgaaca aggattaagt tcattatctt
     1261 caagtgtatt catcattatt acatttattt tctatatccc tatgtcattc ttgattcctt
     1321 caacttccgg cttagctgct gcaacaatgg gaatcatggg acctatgggt gaatttgccg
     1381 gcgtcggtaa ggatttagta atcactgctt atcaatctgc atctggttta gtaaacttaa
     1441 ttacaccaac atctgctatc gtaatgggtg ctgtagcaat tgctcgtttc gatgtttcag
     1501 tgtggtggaa atttaccggt aaattaattg cgatattatt tgtagccatc tgtgtgatcc
     1561 taggtgtagc agcaatgttc taaacctaga caaatacagg tacaaggtct atcataagaa
     1621 tgaagaaacc gataaacggc tatcgataag cacgtggaca agtcaatagc tccattcagt
     1681 gagtgttttt tctttcatca tttgttaaga acattctgct tgctgatgga gcaggcttaa
     1741 aacgtcatta tttgagaaga agaggttgtg tcggaggaat ttgacatgac cctttttaac
     1801 atattaggag gataatatca tgaaaaaatt tgtgacagaa caacatcatg aacaagcatt
     1861 agaatcattg agcgaattga tccgaattcc ttccgtacta gatgaagcag attcaggaca
     1921 aggacatccc ttcggtaaaa aagtgattga agctttggat aaagtattgg aaatcagtga
     1981 aaagctagga tttagaacat ttaaagatcc agaaggctat tacggttatt cagagattgg
     2041 ctctggagat gaattattcg gtatcttgtg tcatatggac gttgttccag caggggacga
     2101 aaataattgg gaaacaaaac cattcgatcc aactataaaa gacggctggt tagttggtcg
     2161 cggatcccaa gatgacaaag gtccttctat tgcggcaatg tatgccgtca aagctttgat
     2221 tgatgctggc gtggaattca agacacgtat tcgttttatc tttggtacag atgaagaaaa
     2281 tttatggcgt tgtttagaaa aatataacga aaaagaagaa ggaattacgc aaggatttgc
     2341 gccagatgct gaatttcctt taatttatgc agaaaaaggc ttattgcaag cttacttgac
     2401 aggaccagga acgaacgagt tttctgtaaa agctggagga gcgttgaatg ttgttcctga
     2461 tgcagctcct tactctggag aaaagctttc tgaagtaaaa gaagcattga aaaaacacga
     2521 attcgatttc gaagaacaag gtgaaggcat cgtcgttctt ggtaaaagta tccacgcgaa
     2581 agatgcagca caaggagtga atgctatttc tcgtctggca attgctcttt cagaagtatt
     2641 tgattttggc ccaatcaatt tcttaggaaa attagttcaa gaaaatgcaa ctggtgaagc
     2701 tgtcgtgggc aaaacagaag atgaacaatc cggcgagcta acgatgaact ttgcaagttt
     2761 ggaaatcaca ccagaacaaa caaaaatcgg tgtagatatg agaatccctg ttacattcaa
     2821 gaaagatgat ttagtagcta aattaactaa aacagcagaa aaatatgggt tgacttatga
     2881 agaatttgac ttccttgatt cattatacgt gccactagac agtgagttag tgaaaaatct
     2941 attggggaca tatcgcgata ttactggaga catgactgaa ccgtttgtat caggcggcgc
     3001 tacttttgct cgtacgatga accagtgtgt ggcattcggt gcgatgttcc ctgatactcc
     3061 tgactttatg caccaggcga atgaacgatg ggaattaagt agtatgtata aagcaatgga
     3121 aatttatgca gaagctgtgt atcgtttatg tgctaaataa aaataaacat acaaaaaggg
     3181 cggatattca aatggatatt ccgtcttttt ttatttgatg ctggaaagtt tgcatttctc
     3241 tcttattttt ttaaggtata atcatacttg attaagaaaa aggggtcact cagatagaaa
     3301 aggtgggaga aagtgaaaat atttagacga atactaattg gcttagtcat cattttagga
     3361 ttactcggtg gagccggtta ttactatata cagcaaaata cgtacgaacc atcgtcagaa
     3421 gcgcaagtgt tggtaaaaga aggaaaaaac aaagaagatt acttattttt tgaaggagag
     3481 aaaaagaatc atggaaagtc tccattactt attttttatc ctggtgctct cgtagagcca
     3541 gaaagttata gtatttgggc gcaagattta gcaaaaaacg gcttttcagt cgctattgtc
     3601 aaaatgccac ttgatctagc agtgatgggc ggaaacaaag cggataaggt caaagaagat
     3661 tttccaaatt ccgattatgt gatcggtggc cattcattag gtggcgtaat ggcaagtcgt
     3721 tatgtcaata agaatcattc agacaaacat ttaaaagggg tattcttcat ggcaagttat
     3781 cccgacaaaa aaggaacatt gacagaatca gctgttcctg ttttatcgat cacggcgaca
     3841 aaagataaag tattgaatca ggaagcttat caagaagcta agcagtattt accggaaaga
     3901 acagagtatg tatcaatcaa tggcggaaat catggaggtt ttggcagcta tggtgagcaa
     3961 aaaggagatg gagaggcaga aatttctaac gcagaccagc aaactgtgat cgtcaataca
     4021 ttggaaaact ggctgaaaaa gttgaaataa tataaatggg tcctataggg tccgtgaaaa
     4081 aactagtcaa ttaactatag atagcaaatg aatctttcaa aataagtcgg gatttcacgg
     4141 aacaagagaa acagatcccg gcttattttc aagtttagag aacttagtgt acgtttttaa
     4201 agtttcgttt atatttatat tttattttct aataaaatcg ctcgaactgg acatttttga
     4261 tacgcttttt ttacatatgt ttcttcttcc ggcatcacga actgttgatg agcctctggt
     4321 tcttgggcaa ataaaacgat tccttcttca tcataatcga aaatatctgg cgcttcgatt
     4381 tgacaaagac cgcatgcaat acagcgttca ggtacaagtt tacatagtag tgacatttct
     4441 aaccatccta acttaaggag aataatatga ttgtttttat tttatcgcta ttttcatcag
     4501 gacacaagct gcggattagt tctctttacc aactccttgt gggaaaacga acgacttcag
     4561 tacttattta tggatttacc cacgaacttt tgtttatcca taacagtttt ccagaattga
     4621 agcaggataa gttttatcaa attttgcaaa aaattgcgca gcaaggttgg atcgaaataa
     4681 atgaaaatga agcaaagcta acgcctgcag gggctgatat gttgtccgaa catcaaatag
     4741 aacatactgg tttgcgtttt gaccgctatg gcagaacagg ggaaactagt tggcggctca
     4801 ttaaatttgc tgttcaagta atttctaatc tggcaacggg tattcaagac tatcttccag
     4861 cagagacaag tcctttttat actttccaac taaaaaaatg gcttagtgaa agccaattgc
     4921 cacgtgaaat tttgattgat actgcgtatg agagtttggt gcagctgttt tcggagatac
     4981 cagaagaagc agcagatttt ttagctaacc aattatctgg aaatgagcga acagggctgc
     5041 ttccttatca gctggcaaaa aacaatgatg agtcagaggt ttatctgcaa caaagcagat
     5101 gtatccacct tttgttagca caaatcgaaa aacggccaga ttcgctttgg cacgctttga
     5161 tcgattcatt attacagcaa aatttcaatc aaagcatgat gatcaccaag cagatgttta
     5221 tgaatggtca gacaattgac cagatcatgg caatccgtca tttaaaaaga ggaacagtaa
     5281 cagatcatct tattgagtgg gccttgtttt ttgatgattt tccatacgag tggattctct
     5341 ctgcagaaac aatcgaacgt ttggaaccaa ataaggattc cgtgcgagag tggcgcttta
     5401 gcgaatggaa tgtagatgga caattagatt atggagaatt tcgtttgtat caaatctatc
     5461 tattgagaaa ggaagcgata cagaatgtta ataaatgaat taaaaaaata ttttggattt
     5521 gattcatttc gtcccggcca agaagaaatt attcaggcgt tgcttgatgg agaagatact
     5581 ctcgctattt tgcctacagg aacgggaaaa agcctttgct atcaattgac ggggtattta
     5641 atggaaggtc tggtcatcat cgtgtcaccg ctgctttctc taatggaaga tcaagtgacg
     5701 caattacaaa aacgagggga gaaacgagta gctgctttca atagtttact ttcgcaatct
     5761 gaaagaagat acgtcctccg tcatctatcg caatataaat ttttgttttt gagtcccgaa
     5821 atgttgacta atccttctct tttagagcat ctgaaaaaac aaaatattgc gttatatgta
     5881 gtagatgagg ctcattgcgt gtctcaatgg ggagttgatt tccgtccaga atatcaacag
     5941 ttaggaaaaa tccgaaagct tttaggaaac ccggtcacac tggcactaac ggcgacagca
     6001 acagatttag tagcaagaga tatacgtcat gtactatttg atcgaccacc aaaagagatc
     6061 agacaatcag tcaatcggca aaatatctcg ctttttgttc ggaaaacgca gcaaaaagaa
     6121 caagaacttg aacacttcat ggaaagagca caaggagcgg caatcattta ttgtgcaaca
     6181 aaaaaagaag tcgaacgatt gtatcattta ttcagggaac gttttacagt tggatactat
     6241 cacggtggtc ttgatgcagc tcaaagaaga caacttcaac agcagtttgt aaaaaatcag
     6301 ctgcagtttc tcattgctac gaatgcattt ggtatgggga tcgataaatc tgatatccgt
     6361 tatgtggtcc attatgattt gccagacagt ctagaaaact atgtacaaga aatcggtcga
     6421 gcaggcagag atcaggaaga aagtgcagca attctttttt atcaaagtgg ggatgaacgt
     6481 atccattatt tctttaatca actttctaga gaacagcgac aaagttttga gctttatttg
     6541 gaatatgcca caaagcaagc tccctttgat gagctgcaaa aaaaatggat ggagctgatc
     6601 cagcagtctg aaaaaccaga aaattgggtg gaacggctca aacgtcagga aaaagaaaaa
     6661 gaattccgcc tgcagcagat gcttcgctat attaatgaag aaaactgccg cagaaaattc
     6721 atcttagctt attttggcga aaaactctcc gaaaagccgc agaattgttg tgatattgat
     6781 ggagcaaaag cagaacttct gccaaagagg aagctttttt ctaagactga acaacttcat
     6841 tgggaaacga ttttactaaa tttatttaaa aaaaacatgg aacattgatt ttttaggaca
     6901 agacactttt tcctatggta taatacacac gatagagttt aggaggtaaa acagtgagca
     6961 gaaaagatag acatcaatct acagaaaata acgaaacaca agaaccatgg gaacaaccaa
     7021 tctacgatac agatgatgaa gtttcatcaa gaagttctca gagacaacaa aagaaaggaa
     7081 atacattatt tctttcgcta tttttgattt tactggtctt atgtatcgct attccagcag
     7141 gtgcttattt ctggatcaga aatggatcaa gcaacaatgc aacagcagca acttcttctg
     7201 aaacttcgac atcagtagta gaaagttcta caagtacaac aaaagaggag tcaactgaga
     7261 cttcaacagt agaatctagt acaatcgcgg aagaatctac tgtgtctcaa gatacaggtg
     7321 taacgtcaga aagcagtatt gcagcagaga caacaccgac ttcctcaagt gaggcaactt
     7381 atcaagcagg ggattcaagc agcaatgcag cagaaacaac accttcttca tcagctgcag
     7441 ccggttcaac aacgactgtt caagcaggcg aaggtccaaa acaagtggct gagcgtgcag
     7501 ggatcaccac tgatcaattg tttgaattga atggcttaga tccaaacaac tttatgcttt
     7561 accctggtca agaattaaga attaaataat aatagccgcg ctttgcggct ttttttctga
     7621 gaaaaaggag ttatatcatg acacaaatca gtattgcaat cgacgggcct gcttcatctg
     7681 ggaaaagtac cgttgccaag atattagcaa aagaattgaa ttatatttat acagataccg
     7741 gtgccatgta tcgcgcagta acttatttag ctattacgaa caatgtatct ttttcagatg
     7801 aagaagggct agtttcgctg atcaaaaagt atcctatctc tttttcgcaa caagaagatg
     7861 gacagcatgt attcatagca tcaaaagatg tgactttaga aatccgtcaa ccagatgtca
     7921 caaaagctgt ttctgaagtc tctgcacatg gaaaagtcag agaagaatta gttgctgtcc
     7981 aacgaaaaat aggggaacgc ggtggcgttg tcatggacgg aagagacatc gggacagctg
     8041 tattaccgaa tgcagaagtg aaaatctttt tagtagcaag cgtaagtgag cgtgcagaaa
     8101 gacgtttcaa agagaaccaa gaaaaaggaa ttccgactga tcttgaaaca ctgacgaaag
     8161 agatacagga aagagaccat tatgattcaa caagggacgt ctctccgctg aagcaagcag
     8221 aggatgctgt tcgtatcgat acgactggca aatcgattcc agaagtcgtt gcagcaata
//
LOCUS       DACENJ010000116         8132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.119, whole genome shotgun sequence.
ACCESSION   DACENJ010000116 DACENJ010000000
VERSION     DACENJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.119"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..771)
                     /locus_tag="IW748_001832"
     CDS             complement(<1..771)
                     /locus_tag="IW748_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1104741.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            complement(791..1426)
                     /locus_tag="IW748_001833"
     CDS             complement(791..1426)
                     /locus_tag="IW748_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1104742.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            complement(1454..2251)
                     /locus_tag="IW748_001834"
     CDS             complement(1454..2251)
                     /locus_tag="IW748_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1104743.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            2567..3178
                     /gene="rpsD"
                     /locus_tag="IW748_001835"
     CDS             2567..3178
                     /gene="rpsD"
                     /locus_tag="IW748_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1104744.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            3318..3857
                     /locus_tag="IW748_001836"
     CDS             3318..3857
                     /locus_tag="IW748_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1104745.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(3932..4441)
                     /locus_tag="IW748_001837"
     CDS             complement(3932..4441)
                     /locus_tag="IW748_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1104746.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4821..5582)
                     /locus_tag="IW748_001838"
     CDS             complement(4821..5582)
                     /locus_tag="IW748_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1104747.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            complement(5579..5800)
                     /locus_tag="IW748_001839"
     CDS             complement(5579..5800)
                     /locus_tag="IW748_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1104748.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            complement(5819..7405)
                     /locus_tag="IW748_001840"
     CDS             complement(5819..7405)
                     /locus_tag="IW748_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1104749.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            complement(7405..8106)
                     /locus_tag="IW748_001841"
     CDS             complement(7405..8106)
                     /locus_tag="IW748_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1104750.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
BASE COUNT         2453 a         1726 c         1454 g         2499 t
ORIGIN      
        1 aatcacgctt aactctttca atgccttcca aattccatcc tgatcgtttg tatcagtgac
       61 catcttagct tctgcttgta cttctggcac agcattcccc atagctatcc caattcctga
      121 catgcgcaac atttcacggt cgttttgtcc atcgccaaag ctgatgatgt cttcatgagg
      181 gatgcctact tgttctgcta agtgcagaat tgtcgctgct ttagaaccat ttttaggtac
      241 gacatctaca ctgttctcat gccagcggac aaatcgaatt ttatcatatg aagagaactt
      301 cccatcatat gtttgatcga aaaatgctaa cgcttggtat atttcttgtt cctctgcaaa
      361 aaactgatct aattcaggca agacagcacc gaaagaatgc atcgcttctt ccatcaccgt
      421 tacatcattg gaagaagaac gcttgatatt gtctaagctg acaaaagctg tatcgatctg
      481 ctcatgatgc gcttcttgaa cgaacgcatc caactggtcg cgatctagta aattcttata
      541 tacttgttca tggtctaaaa aaccggccgc tccattacat aaaatgtagt tcgtaaaatc
      601 aagatcgcga atgatctctt gcgcatgaaa tcggctccgc cctgtagcaa tcgtgaccag
      661 atgaccggct ttgcgtaatt tttccaatgc ttctcttgtg ctatctaaag cttgtttgtt
      721 tgaatctaac aacgttccat caatatcaaa agcaaataat ttgcgtctca tctcttgtcg
      781 tctcgctttc ttataaatac ttacttgcct ctcgaacagc taacttactc aaatctgttg
      841 tcagctgtct ttttacccaa tcggcatcgg tcttactata atcgcgtaat gcccaaccaa
      901 ttgctttttg gataaagaat tcctttgttt gacgatcttc gtctattgct cggatcaata
      961 gtcctgtgtc tgtttgctgt ttataaccta attgcaacgt gattgctatt cgacgtagcc
     1021 aaaaatcttc ctttttttca aacaattgaa agacttcttc acgctgctct gggtaggctt
     1081 tacaccatgt gctgtatact tttctccaag agtcgatgct gtcccaccat tcttttttct
     1141 gaactagtgg caacaatacg actaagtctt caaatgtaag ccgtttgatg tttttctgga
     1201 ctaaatcgat cgctacatac tgatactctc tttcgatttg ttcataataa taatgaacaa
     1261 aagctaaaac ttcagaaata gataaatttt tgctctcatc cagtaacggt tgttgtaaaa
     1321 gagcgcgttc cggcttaggt actccagcaa aggaaaaaag atttttcatg tacttttcca
     1381 tttgtaacgc tttttcctca ttttttggaa aaactaaaac ttccatggca cttccccttt
     1441 aatgaacgac tttttagtcc acataggatg attttgactg attcagccaa gcatatccca
     1501 gtcccattac taaaccgcca ccaatataat ttcctgccaa agcaaaaaac aaattatgta
     1561 acacattgct gactgtcatt ccatccatct gtccgtgaga agcaaagtaa gtaagcgtaa
     1621 aggcaggaaa attcgctatc acgtgttcat atcctaaaaa agcaaaaatg aaaatgatga
     1681 atacgactgt gatcactttt ccgacatcct ctttcatccg catgctgatc agtacagcgg
     1741 tattgacaac gatattcgca aacatccctt ccaccaaaat cgttgctgaa ggttttgcta
     1801 gttttactgc tattgattct aacataaagt tgtccaacgt taaatcttga aatggcgtag
     1861 tcatagaaat tagaaatcca cataatatac cgccaatcat attgaaaaaa atacaggcaa
     1921 atagaatctt ggctgcctta cttaaagcaa gtctctgccg aaaaacaccc acggtcatat
     1981 aaagcatatt cgatgttcct aattcggcgt tcatgtatag aatcatgacc agaccccagc
     2041 tgaacataaa tgcgtataaa atctttccta gaccatgcgc gatatcttcc cccttcatcg
     2101 cgatcgcaaa tgccacagcc gttcctaaag tcaaaaacaa acaggcgaga accgctcgaa
     2161 cagcgtaacg tgaaaagctg ttttcaaaaa gattcgcctt cttttcaatt gaagcatcga
     2221 ttttctcaaa taatggtgat accggattca ttgattttct ccttaaactt ccttcgcttt
     2281 tttccatttg cacataccct ctcattttat aaagacagcc taggtttgta aagcgttttt
     2341 atgtactaat tatcttgaca ttcgtggatg atctatcata tactgtaatt tgtgtaaaat
     2401 aatgcagcag aaaaataata actcatccct agtttgttgc cccgtctcgg gttcgattgt
     2461 gtaaccgcgg ctgcaaaggt gaagattgaa gaacaaacta tatgattatg aatttttcaa
     2521 cggattattt tactccaaaa acttatattc attggaggaa attttaatgt ctcgttatac
     2581 aggaccatca tggaaaattt ctcgtcgctt aggcatctca ttgtcaggca caggaaaaga
     2641 attagcacgt cgcccttacg caccaggtca acatggacct aatagccgtg gcaaacaatc
     2701 agaatacggc atgcaattaa ctgaaaaaca aaaacttcgt cacatgtacg gaatgaacga
     2761 acgtcaattc cgtaacttgt tcgtaaaagc tagcaaaatc aaagaaggta aacatggtgt
     2821 taacttcatg atcttactag aacaacgttt agacaatgtt gtttaccgtt taggacttgc
     2881 aacgactcgt cgtcaagcac gtcaattagt taaccatggt cacattttag ttgatggcaa
     2941 acgcgttgat atcccttcat accacgtaga agtgggtcaa gtgatctcag ttcgtgaaaa
     3001 atcacaaaac atcgtaacag taaaagaagc tgtagaagca actgttggac gcccagcatt
     3061 cgtaagcttt gacgctgaaa aattggaagg ttcattgact cgtttaccag aacgtgacga
     3121 actatatcca gaaatcgacg aagctcttgt cgttgaatac tacaaccaaa gcttataatt
     3181 tcaaaagctt tcttgaaaaa gaccgctatc tattggatag ctggtctttt tttatatttt
     3241 ctatttacaa agtaatcact ttgttttaca atttcgttaa ccaaataaaa atattaagtt
     3301 aacgaaaaga gggcgttatg aaccgaacat tgaaagaatt gatacttgct gcggaatttg
     3361 ccgcgattat ttctgtcctt tctcagatga ccattccttt tggtttggtt cctttgacag
     3421 ggcaaacatt tgctattgga ttaacagcaa cttttctcgg aagaaaacta ggattgatat
     3481 ctgttggagt ttatctttta cttggcttga ttggtcttcc tgtattttca gggatgactg
     3541 gaggtatagc tgttttattt gggccaactg gcggatattt gattggattt ctcttacaaa
     3601 cgtggataac tggttggatg atcgaaagaa cggatgctca ttacttatat gcaatctttg
     3661 ccaatctaat gggctctctt gctgcattga tctgcggaac gatctggctg aaaataagtg
     3721 gtgatcttac ttttacaact gcatttgcca gtggactact gccgttttta ctgccggaag
     3781 caatcaaggc aactggagca gcttatatcg gtatcttgat gaaaaaacgg ctaaatcgat
     3841 ttcttcatac aaattaatca aaaaagcagc atggaagaag actcaacatt cagtctcttc
     3901 catgctgccc tattttaaat taaacagatc tttattttga caaatccact tttttaccga
     3961 tccaataaat aattatccca ccgacaatca ttgatgccaa ttctcctaaa gcaatcaccc
     4021 accaattcaa gaaaaaaggt agttgatata aaatcgttag ctgaccagct actgtaaaca
     4081 tggaaaaagc aaatacgcat gctgtcacgg ccattttgat tttcatgttt ttgatgcgac
     4141 tagtgatttt atagttgata agcaatacaa gcagcgtgct gacgcttcct actgcaacat
     4201 ctatcagtcc aagaggagaa gcaatattag ccaaagctac tcccgctgtt accgcgacga
     4261 tgtatctttt gttatacaag gacagatagt tgaacatctc cgagagacga atctgaatca
     4321 ctccaaagct aaagacagac aaaaccagcg tcactgccac atataaacca gtgactagcg
     4381 ccatccgagt cacatctgtc gttgtccagc gtttggctga acgggtatta tttttttcca
     4441 tgttctattt ctcctttgca agataatttt atcttggtaa tgaccgcgac caaaggatgg
     4501 gaagaaaatt ccaactgaag tgtcgcagaa ggtagtgaaa aagctgccct gcaacaagta
     4561 ataagaatgg tcaaataaaa atagctcctt atatttttca tttggctgga cttgttaccg
     4621 cagttgcaag cttttgaata tcgtttatat caagaggtcg tgaaaagccg cttagtttcg
     4681 aatattaacg attattaaaa agaatttttt gaactggctg ttcggcatcg aatgtcggga
     4741 acagtcatcg acttttttta tcatcaaccg ttgtttctct ctttagtttt atcatacaga
     4801 agcttgaaaa aaacacaagg ttattttaac aaccatccgc cgtcgattgg gatgatcgtt
     4861 ccttgtatgt agcttgcttg aggagaggca agaaataacg tcagctcagc tacttcttcc
     4921 ggctgtgccc atcgcttgac tggtgtttca tttgccaccc actctgccat ctcacctttc
     4981 ccttcaaaat cagccgcatt cattggtgtt tggatggctc ctggagcaat tccttttact
     5041 cggataccct gtcctgcgta atccagagcg agttgtttgg taaatccgac aatcgcatgt
     5101 ttcgcgcttg tataagcgat cccacctcca ccagcaatca agccagcaat cgaagccata
     5161 ttgatgatcg ttccgctttt ttggcggatc atatcaggaa ggatgatttt tgtcagctga
     5221 tacatgcttt tcacattcgt ctcataaatc agatcccaca gcgattcgtc agtttccatc
     5281 aagggtttgt aggcatccaa tatccccgct gtattcagta aaatcgtaac ttcaccaaaa
     5341 tgattttggc atttcgatac cgcttgttgg agttctttca tttgtcgaac atcacctagt
     5401 gcgtaggcaa aagtttcagg aaatcgcaaa gaaatttctt ccattttacc gaacttttga
     5461 tctaatccaa atacaaaagc atcttgttca agaaacgctc gggcttgcgc agcaccaatt
     5521 ccagaagcag ctcctgtgat aaatacaacc tgtttagcta gatctggata ctggtttttc
     5581 attctacgat cgcccaatca tctgctaaaa tatcacaaac tgtaggagcg aagctggaaa
     5641 aaccttcgtc acttgttttg atcaataagt acggcgtcac cggacagcca tcaaattcat
     5701 catcggatac gagaacaaca taaagttcaa atccactcca tcctttacga ataatctttg
     5761 cacctttttt caattctggt aaaatttctt caaatgtcat cttttttctc cttctttgtt
     5821 attgttttaa tagtttttgt ttttgaatca tttcaaattt ttgagatagt tggatgtaat
     5881 caattaattt tagttcttca gtcacaataa aatagtcaag atccgcccat ggtttgcggt
     5941 ggactttact tgtcgaaatc agaagatcaa cttctctccc ccgataatct gttggcagaa
     6001 aaatcagctg atactcatta cggaaaaatg tccgtaatcc ctcctctaac agttgctctt
     6061 caaataatgg gaaatctgtc attaaaagga tcgtgatctt tggaagctgt ggagtaaagg
     6121 ctcctaatgt tttaaacaca gtcagatacc gccccatcaa aaacgcatga tttttaaatg
     6181 ccagatgatt gggatgtttg tgtctacttt caaaaaaatg tacgagccat tgtttcaatt
     6241 ccttgttttc aataactaaa tgattaataa aatcactact attgccatca atcgtttctt
     6301 tcgcattagg aaaaagatag gcgaaaaaat gactgctgaa taaatagttt tctgcctcga
     6361 aggttagatt ttctgctggc agcccctgct cttcctgata aagagaaaga gcggcttttg
     6421 cttctgaaag ggctgtaaag ggcggtgttt ttgcttgttg atgaaaatca aaaattttct
     6481 tccgtatctt aggatcacta taatattctt cacgagtcat cagtacaaga aataatgcgc
     6541 ctaactcgtc ttctacttga aaataattag gaaataccgg ctccatgacc tggcaaaatc
     6601 gctggaacaa cagattgtca gaaatcagcc gtcttttttt ctcattcaat acaacataat
     6661 gcttctgtga ttttcttaaa aaaaaggcac ctagcatata ttccaatcgt ctttttttga
     6721 tctcgttcag acgaacatta aaaaaagcca tgatatgttc tgccatattc tttacgtcca
     6781 tttgagaaat cgtttcaaat ggccagattt tccctcgaaa caactgccag aatgtcatat
     6841 gcaagtaaag tcgaatgatt tcttcttcgc cttgagcgat atatgtacct cgttgcagcc
     6901 gaattccgta agtttgcagt tgctgtttcc attcattgat ttttcgatga gcagtagatt
     6961 cactaatgta atgcttttgc gcaaattggt acattgtatg gaattcttca aaaaaaatgt
     7021 cgtgcagcaa ctggaatttc agcgtacttt gcacgagata gatcaaaaaa cgctcatgtt
     7081 caattgaatc gagcgtatca taaaagttat aaccagaacg gtgatttttt gtaaacaaag
     7141 ggtgtgtctt accgctaaac tgctgatagt ttttagacag ctcgttcagg tatttcgaca
     7201 cgctgcgggc atccagctga acaacttgcg ctaattgtgc agcggtatgc caagcttgat
     7261 cctcaaaact ttctaggagg agaatcttat aatatagatc tttatcaaat tgtttaagat
     7321 actcggaaga caaatacacg tctttccctc cttttttatg ctcgaacgtt ttggttattg
     7381 tagcataaaa tagtggtttt cgcatcaaaa caagccagca aaaggctgaa agaataacgg
     7441 tagcaataaa acggtcaata ttccgttgac gcatatcgca agtccggaaa tgattccttc
     7501 tatttcacca atctcgattg cttttgctgt accaatagca tgagaagtac ttcctaacgc
     7561 cacaccgcga gcaactggac tttcgatctt gaataggcgg aaaatatgag tggcgatcag
     7621 cgaaccaaaa atacctgtag agacaacaat agcaagtgtc acggcattca ccccgcccat
     7681 cgtatgagat aaatcgacag aaatagccgt cgttacagat ttaggtagta atgagatcac
     7741 catctcttta tgaagagaga agaagtaacc gaaccctaca ctaaccaagc aatttgcgac
     7801 tattcctgct aaaatcgcag ccattacagg ataaaaattc tctttcagta aacggaagtt
     7861 tttataaaga gggatggcaa gtgccactgt cgcaggagta atcagcatag tcaaaaaatt
     7921 gcccccgata ttatagtctt tatagctgat cccagaaaaa tgcaaaaata agataataac
     7981 aacaatcgat aatacgagag gattcaaaac aggcagttta tatttccgca aaaatttgtc
     8041 agataatacg tacactccta aagtcaaact taagccaaat aatggattca caaaaaactc
     8101 agtcaatttg attctcctcc atttcgttta ca
//
LOCUS       DACENJ010000117         8106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.120, whole genome shotgun sequence.
ACCESSION   DACENJ010000117 DACENJ010000000
VERSION     DACENJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.120"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            46..231
                     /locus_tag="IW748_001842"
     CDS             46..231
                     /locus_tag="IW748_001842"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ1104751.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            474..1994
                     /locus_tag="IW748_001843"
     CDS             474..1994
                     /locus_tag="IW748_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1104752.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(2181..2753)
                     /locus_tag="IW748_001844"
     CDS             complement(2181..2753)
                     /locus_tag="IW748_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1104753.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(2760..3422)
                     /gene="rpe"
                     /locus_tag="IW748_001845"
     CDS             complement(2760..3422)
                     /gene="rpe"
                     /locus_tag="IW748_001845"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1104754.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(3438..3953)
                     /locus_tag="IW748_001846"
     CDS             complement(3438..3953)
                     /locus_tag="IW748_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ1104755.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(3955..4239)
                     /locus_tag="IW748_001847"
     CDS             complement(3955..4239)
                     /locus_tag="IW748_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1104756.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(4273..5601)
                     /locus_tag="IW748_001848"
     CDS             complement(4273..5601)
                     /locus_tag="IW748_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1104757.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(5615..6070)
                     /locus_tag="IW748_001849"
     CDS             complement(5615..6070)
                     /locus_tag="IW748_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1104758.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(6073..8019)
                     /locus_tag="IW748_001850"
     CDS             complement(6073..8019)
                     /locus_tag="IW748_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1104759.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2650 a         1538 c         1283 g         2635 t
ORIGIN      
        1 atggaatgga taaatatctt ttgtatacct tgttaaaaga gttgaatgcc aatgctcatt
       61 gatgcaactg cattgaaaca tatttttatc gtttgtggaa aaacagatct tcgaaaagga
      121 attgatggac tagcagcttt cgttatagaa gaatatgacc ttgatgttta cgatcaagcg
      181 cttttcttat tttgcggtac gaagaaagat cgcttcaagg ctctttacta ggatcaaaat
      241 ggatttgtat taatgtacaa aagatttgaa agtgggtacc ttcaatggcc gcgttccaga
      301 aaagaagtct tactgactgg cctttcaatc gaagaaaaat gtacgctaaa agaaacgaaa
      361 aaaggtgcga tatattagac gaaaaccttg ttaaatcaag gattttgtct gtttttcgtt
      421 gtcataaaat ggtataatag atgtaagaaa atcgctagaa tggtggtgta aaaatgacaa
      481 aaatggaatc tgaacttctt gtacaaatca agcaattaac acagttgctt gaaatccagc
      541 aacaggaaaa tactcttctt agagaacaaa ttgctgaaat gaatcgacgc ttgtttggac
      601 gaaaaaaaga aacaccaccc gtagatgggc aaatcgattt attagacgac tcaactttta
      661 atgaaccaga gcacactgga caagaaagcc aggaacccat tacggtatct tcttttaaac
      721 gcagaaaacg caagggactc aaagcacttt cattagaagg tctgcctgaa gttcctattc
      781 attatgaatt aaaaggtgcc gattgtttgt gtgaagactg tggtcatact ttacatgata
      841 tcggtgcgac aaggcttaga gaagagccat tgtatgttcc tgcccatata gaaaaacaag
      901 tcatctacca acatgcctat cattgcagag aatgtgaagc attaggagaa acaaaaatca
      961 aaaaagcgcc tgttcctaaa ccgttgatca ataatagtct aggttcctct tcaattgtga
     1021 cacagagtat ttgggaaaaa tttgttaaaa aggtgccggc atatcgacaa gaaaaagaat
     1081 ggcatgcact tggtttccct cttgatcgta aaaaaatcac taattggcat atcaaggtaa
     1141 gtgaaatggt tttagagcca atctacaatt gccttcatca agaattaagc aatcaggagg
     1201 tcttacacgc ggatgagaca agctatcggg tattgaatac tcataaagaa aagacctttt
     1261 actggttata tgcttcaggt aaaagcgaag aaaaacaagt tgtgctctac gagcacgcaa
     1321 attcgcgttc cgttgatgag ccaaaaagtt ttttagaatc atttagcggt ttcttgcata
     1381 ctgatggtta cgcggcctat ggaaagctgc cggtaaacaa tgtttattgt tgggcgcatg
     1441 ttcgcagaaa gttttttgaa gcattgggta aacaaccgtc cccaaaaagt catgccaaaa
     1501 taggattaga cttctgtgac aagatgtttc gaatcgaacg gacctttaat aagcttagcg
     1561 cagaagaaag gtttgtagcg aggaaggaaa cgttgcagcc tgtgatgact gaattttttc
     1621 attggtgtga gagttttgct tatttgccaa aatcgcagct tggaaaggcg gttcaatacg
     1681 cagtcaatca taaagacgga cttcagattg tgttgcttga tggacgcttg gaactttcaa
     1741 ataaccgagc agagcgtgcc atcaaagagt tagtgatcgg tcgaaaaaac tggctatttt
     1801 ctaaaagctt aaaaggcgct agatcaaatg gaattatatt gagtattatc cagacagctg
     1861 ttgcgaatgg gttaaacatc cgcaaatatt taaatcatct ttttacagag attcccaatc
     1921 tgtcttcgat gacacctgaa gcgctacgcg cttatttgcc atggaatcag cagatacaag
     1981 aaatctgtaa ataatataag ccacaaataa cctgatttga gtgtatcaaa tcgggctatt
     2041 tgtgtttagg taccattcta tgacacgctt acgctcaaaa ctactgaatt attaagttat
     2101 gtctaatcaa ataaaaaaac cattctcata tttgctgaat aattagcatt tagagaatgg
     2161 ttttttatct ttaaaatcta ctactcaaaa tctgcatgtc gagcaaacat cgtttctgag
     2221 ctttcacctg ttttatccat taaagataac actaaactat caaaggtgag aaatgctaat
     2281 tgttcaaaac ttgaacccat aggttgtgaa aaagtatctt cttctcgttg attttcatct
     2341 ttggtaatac ctggtaatac aagaacagaa tctgctaatt gaccaatcga cgagtctttt
     2401 ttcatggtaa ctaaagcaat gcttacacca ctttgatttg ctttggccgc taaatttttt
     2461 aaactacctg tttcacctga gccagaacag ataattaata aatctccagg ttgcgaatgt
     2521 ggtgaactaa tttcaccgac aaggctgact gaaaaaccta aatgcattaa gcgattcgta
     2581 aatgcttgca tggcaattcc agagcgtcct gcaccagcta gaaagatgtg tttagcttga
     2641 acaattttct cactaaattg ttctaactct acagaattaa tacgttttga gttattttgt
     2701 aattctaaga taatcgcttc taaatagttt ttattttggt ctttattggt catgatcttt
     2761 tacttcactt cctctaatga ttgatttagt ttatcaattt gttcttgcgg ggtatttttc
     2821 ccattgaaga tgaaaccgcc tgatacaaaa atatccgccc ctgctttaga acaaacttta
     2881 atcgtttgat tatcaatctt gccatctact tcaatatcat acgtataatc attttgtttg
     2941 cgtattacat ctaactgttc gattttaact actgtctctt gaataaaatg cttattctct
     3001 gttcctggat tgattgtcat tactaatact tgatcgacta aagataatac tggttcaata
     3061 aaactgactg gtgttgcagg attaaaagta atccctactt ttgctcctgc ttccttgact
     3121 aactgaatga ccttcattgc gtgtttggta gcttcaatgt ggaaggtaac aatatctgct
     3181 cctgcttcta caaatcttgt gacaattttt tctggtgtat caatcatcaa atgaacatca
     3241 aacacaacgt ctgttagtgg acgaatatct ttaataacac ttggtccaaa agccatatct
     3301 ggtacaaaac agccatccat tacatcaata tgcaaataat caacattctt ttcttttaag
     3361 atttcaaata actctttaac ttctgcgata ggtgcattta acaatgatgg agcaattgtc
     3421 attctctatt cccccgttta ttctcccata atcatgagat tgattgttct agttcgctct
     3481 cttagttgat cccaatcgac aaaatagaca cggcccattt ctcctaataa aagtttacct
     3541 tcttgaacaa ttaaagtcat tgagttggtt ccataaatag atgaacgtaa atgtgcatcg
     3601 gtatttagca tgacccattt ttctgcaggg taattgggat cagataatct tgttccaaaa
     3661 tcaatgtgtt ttggccccgg actgttgtat tgattttcac tcgtcatttt tggaataatc
     3721 gtttccatta cattgttgat gtctacatgt aaataatcat caccaaaaaa gtttgtgtca
     3781 tgcatcgttt cttcaaaaaa gagtgagcaa gttgtatgcg ggctcgaaat tactaacatt
     3841 ccattctcaa tacctgctgt atcaactgta tctaaaagtt cttttcgaat atcatgatac
     3901 gaaggacgcc ctgaaactgt ttcaatagtc aaagtgtcat gatgaaaacg catattaaac
     3961 acctaatctt tcttttacat ctgcataaat ggcatctgta ccaatacctg ttaataagcc
     4021 caagccattc gttactttag ctttaattac atcagggaca attgttgtac ttactacagc
     4081 atcatattca tcaattttat ttagctcatc agcaatttta gattgataaa ttttcacttg
     4141 atctgttaat ccttcttctt ccaaccattt tgataatttt gctactgcaa ttgtagaagt
     4201 agcatgacct gctccacaaa taactaataa ttttttcata ttgatctctt ctttcttatt
     4261 ttaattttat gattaagctt tcttcacttt gttcatgtag atagaagcac ctactaaaac
     4321 aactcctaat ccacctattc caattacacc aaagtaagat gctaataaaa caaacaaccc
     4381 tactggccat aatccacata acaacatagt tactaatcct gctgtcccca catcgaaact
     4441 tgctaatgta aacacatcag tgataattgg tgccaaccat gttgccatat ataatgttgt
     4501 aacaacatac aacgaacatc ctactaatga tctcacaata tttcctttaa aataaggaat
     4561 cattaaacag atggcgaaag caaagaatgc taaatctcca aaaggtaata ctttatttcc
     4621 tggaagaatt agtgctagca ataatgagat tggtacaagc aatagagaag ttgacataat
     4681 agttggatga cccacagtta acgctgcatc cattccaatg tttacttggc gaccatttaa
     4741 ctttttattc gtaaattctt tcgccgcttc tgcaattggc attaaacctt ccataaacat
     4801 tgctaccatt ttaggcataa tcttcatcac tgccgcagtc ataattccta agtttccaat
     4861 tcctgctacg tcatagcctg ccataatacc cactacaata ccgataatta ggccaataac
     4921 aactgtgtca cctaaaacac cgaaacgttt tgttaatgtt tctgcatctg catctatttt
     4981 attaaatcca ggaatacgat caaaaaccca atttactggt ttggcaaata gtactcctga
     5041 taatcccatc atatgtgtga tagagattcc tggatagccg aaaaattcgc taacagtttt
     5101 ttgtgtacga tctgctaaga accattgtaa aattactgcg acaatcgcgc caattaagcc
     5161 aaaccaaaag tttcccgtaa cagattgagt aatcatacct aggaaaattg cgaaccaaaa
     5221 gttccaaata tcaacattca atgtttttgt ccaacccaaa accacaaata tgaagttaag
     5281 aattaatgaa attggaatca ttaaaattcc caatgatgta gagtaagcca aaggtccacc
     5341 aacacctacg ccaatatcta ggatattcag gccaccaccg tattgttccc ccatttgttg
     5401 aattgctgtt cctaaaccat ctgataataa acttaaaact aatcctaaac caatagaacc
     5461 gataccaaca gtaacgccgg aaacaaatgc tttaccaggt tttaatccaa ataatatccc
     5521 gattacaaaa ataatcagtg gtaaaaagac gtttgaccct aatccaataa accattcaaa
     5581 ggcatttaat acaatatcca tctctctatt cctcctaata aactcctgca gaatgtacta
     5641 actcaataaa tgcttctttc gtggtacatg caagtaattc atttactttt tcttcatttt
     5701 gaaataggct aactaagttt tgtagtgtat caatttgttc atgaggttgt gacattgcca
     5761 acataaagac taaacgtaca ggtatttcat gagatttatc tgtcatatct gaaaatacca
     5821 gtggttcctt taatgaggca aaacagattt gagagctatt tacatgtgcg gaatctgtat
     5881 gtgggatagc aaacccaatt tgatttactt ctaatccagt tggaaattct tgttcacggt
     5941 tggtgatacc ttctaagaaa ctatctttca cgcaaccagc gttttttaat tgttcagaca
     6001 ttacttgaaa caattctttt tgatctttca cttctaattg aaataaacat aattctttat
     6061 ctagatacat ttttattctc ctttttgttg aaaaattctt agtaattttt cgctatcatg
     6121 aacttgtaac aagcgatcga ccatttcttt ttgcccaata aattggtaaa gttcttcata
     6181 tactcctcta atctcagata catcttcttc agctaaacta accataatga ctaatttgac
     6241 ttttcgatcg ccccaattaa tcgcattttt taatgctaca ataaaaatgt tggattcaat
     6301 gaccattgat ggattcgcat gcggtaaagc aacaccactt tctaaattag tattccccat
     6361 tttctctcta gagaagatag aagcacgaaa ctcttcagta actaatgaat tggcttctag
     6421 ttcggaaatc atcaaatcca aacattcttc tttgctttcc acctgtactc ctgtttttac
     6481 taaagatgat tgaacaaatt tagatatggt gggaacttta accgctttag gaatcagttc
     6541 attttgaata aatcgcgaag gttcttctat taaatattta gtataagctt ccataatatt
     6601 catataatct gttttactta ccaatggaga tacccttact aagggtatac cacctgcatc
     6661 aaaggaaaga gaggaaataa tcaaatcaac ttccccaatt tcagctaaat gaaacttatc
     6721 taaagttgat atttctacat tatcttttgc tggtaaaaac cgtcgaactt tattgtaaat
     6781 taattgcgca gaagaaatac cgtattggca aataatgatg atattatttg ctttggactg
     6841 atcttcaata gcaatctgaa aatgaatcaa aattaaggaa acttcatcgt ccgttaaagt
     6901 cacatcaaat tcttcttcaa ggatggacat ggcgtaccat actatgctaa agagttctga
     6961 atattgagat ttgatatttt ctaataacgg attttgaatt ttaatacctt tttttaaacg
     7021 aaggaccatt gctggcacat gatacaataa ggattgatat aatcgatcat tttgagtcaa
     7081 atcaattttt tcaatctcac tcattctttc aatcaattta tgaacgctca tagcgtactc
     7141 ttcattgctg gtttttaaat tatttgtcac acgatgtgca aatagttgac gacatagata
     7201 ttctttatct gcgtcataaa atggaagatt caattttgcc ttcaattctt caattaaacc
     7261 atttgccaca atgtacgttt ccatatagcg aatgctattg aagaggaatt cttcttcaac
     7321 agttaaatga tgacctttca gtaaacggtt acattggata actaaagtag aaatcaatga
     7381 ccgaacatag taatcagaaa ccttttcagt cagttcactg taatcattga atagcaaatc
     7441 atgaatgaca ctaataactt ttgaatcaaa caacatattt aaaacttgaa aaaatgtttt
     7501 tgattccatg tcattcgcat aatagaatac aagttgcttg attgctcgtt gaattgcttg
     7561 ttcattacca gtaaaactaa ttccttctgc agatgactgc aacttaactt ctccactgcc
     7621 aataatttta ttgataattc tcaggtcatt gtataacgat gttttactaa ccataaaact
     7681 ttgagacaaa tgagttaaag aaacaggctt atcaccaaac aaaacttctt taatgatctc
     7741 gacacgtctt ttttctggag aataaatatc tttttcaaga taattttcta ccgatgaaat
     7801 caaatgttca acgtttttcc tatcacctat gagtttgata cccaaagaag gtaatcgctc
     7861 aattgttact tggtaattaa ctacataatc ttccaacaat tcaatatctt gataaatggt
     7921 cttggtcgac actgccaatt tatcactaaa aaaagaagcg ggtttaaagt cttcttgtcg
     7981 acttaataat tttaataaat ttacatgtct ttcaatcaaa cttgttcacc tcttaacgct
     8041 agcatttgtt acctcttttc attatttatt ctagcactgg aacccccttc cctcaattac
     8101 atctat
//
LOCUS       DACENJ010000118         7999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.121, whole genome shotgun sequence.
ACCESSION   DACENJ010000118 DACENJ010000000
VERSION     DACENJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.121"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(212..709)
                     /locus_tag="IW748_001851"
     CDS             complement(212..709)
                     /locus_tag="IW748_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1104760.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(749..1558)
                     /locus_tag="IW748_001852"
     CDS             complement(749..1558)
                     /locus_tag="IW748_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1104761.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1892..2749
                     /locus_tag="IW748_001853"
     CDS             1892..2749
                     /locus_tag="IW748_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1104762.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2742..3644
                     /locus_tag="IW748_001854"
     CDS             2742..3644
                     /locus_tag="IW748_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1104763.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3677..4027
                     /locus_tag="IW748_001855"
     CDS             3677..4027
                     /locus_tag="IW748_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1104764.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4061..4999
                     /locus_tag="IW748_001856"
     CDS             4061..4999
                     /locus_tag="IW748_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1104765.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5077..5970
                     /locus_tag="IW748_001857"
     CDS             5077..5970
                     /locus_tag="IW748_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1104766.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6008..6571
                     /gene="amaP"
                     /locus_tag="IW748_001858"
     CDS             6008..6571
                     /gene="amaP"
                     /locus_tag="IW748_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1104767.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6585..6773
                     /locus_tag="IW748_001859"
     CDS             6585..6773
                     /locus_tag="IW748_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1104768.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6786..7688
                     /locus_tag="IW748_001860"
     CDS             6786..7688
                     /locus_tag="IW748_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1104769.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7720..7962
                     /locus_tag="IW748_001861"
     CDS             7720..7962
                     /locus_tag="IW748_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1104770.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         2777 a         1399 c         1562 g         2261 t
ORIGIN      
        1 gaaaaatagt tcctcatatt ttgccgtttg ttaagctaat accgagggtc agctgattga
       61 acaccattta agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa
      121 tctactttga tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt ttttattgtg
      181 tgttaaatat cgtaaatgaa gatagatatt tttatgattc gacgatagag acgttgaaat
      241 ctttcaacag ctctaaagcg gattctccgt gttttctact ggagactaac attggatcaa
      301 caaaaaagtc tgcttcagga aaagcactct gcaggcctat cagattagag agtacacaca
      361 agtttgtttc aatcccgact acttcgatct ctttttgttc ttctttgctt tcaaataaag
      421 tttcctggat ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc
      481 gttcattttc ttgtggagaa agaagaggga tcaattgcaa atcttctcgt tcttcttctt
      541 tgtccttcag ttcaactggt atatcccgtg tgaagagaat atattcatta ttttggcgtg
      601 ctttagctaa tctttgagca atacgttctg ccaattgatc tgactctgga atataaaatt
      661 cgctgtttgg atcaagaata tgattttgca tatcaataat aactaacatt ttctttcctc
      721 ctacattcat atcgtcattt tattcttttt aatagaagtt ttttaaaata taagcaacac
      781 cagcttcttc attcgtcagt gttacttggt ttgcaatttc ttttacttgt cgctgtgcat
      841 tccccatcgc aatactcaac ccgctgtaat tcaacatatc aatgtcgttt tcttgatcgc
      901 caaaagccat gatgtcactc gattcgattc caaggtaatc cgccagaaaa acaagtgaat
      961 gagctttagt gatacctgta ggcgtgattt ctaagaaatg cggatcagag aatactgatt
     1021 ggataccatc tgcttgcaga tctctttgta gcttctgtag tttttcttca ctcttatctg
     1081 ttaatgctaa tttaagatag ctaagagaag gatcttccaa catatttaaa atttgatcaa
     1141 aagaatggtt ctgtaaattg aaatgttcat aataagggtc atctttgact agatttgatt
     1201 catcgaatgt cagctgatca tcttgggttt ctcggctagc aatgatggat attttcgctt
     1261 cttgagcata gaagaaggct tttctcagtg tatcgctatc tatttttgaa cgtctgagaa
     1321 ctttcgccat ttttccatcc ttcatttcag caatcagtga accattggaa gaaatcaaat
     1381 agccaccgat tcctaattcg gaaaaaatat ctttagcaga aatgctgttc cttccggtag
     1441 cgacggttac gattccgcca ttttcatcaa atttccgaag tgcttggata ttttcttctg
     1501 gtatctttcc atttcgatca agcaaggtac cgtctaaatc aatcgctgct agtttcattt
     1561 tatcttccta ctttcttttt acatatctat tttcctgtta ccttaagatt aaatgagaat
     1621 ttaaaaaaat gaaactaact tgctttgtca ttatgaattt tttcaaaaaa cattcttaag
     1681 aaaaaggtgg ctatatatag tagtttttgg tcaatacctt tgaataaaaa gccatgaaaa
     1741 acgaatagtt agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa tattcaatga
     1801 aaacaaagca tatattttgc tttaaaacca aatgaggaat attctagatg tagaacaggt
     1861 tagaaaaaga ttagaaaaag gagtgatttt gatggagaag agatacggcg tagttcctgt
     1921 ggtaactgct tcagacgaaa attatgctcc ttacttgagc gtgatgatcg caactgcatt
     1981 agaaaattgc aataaaacaa gacgcataaa attttatgtg attgacgatg gtctatcaga
     2041 atatagtaaa gaaggattgg aagaaacggt aaataaatat tcaagtaatg caagtatcca
     2101 attcctgaca gtggaaaaag atatctacga agacttccta gtcagtgacc atatcacaac
     2161 taccgcttac ttaagaattt ctttaccaaa cttattagct aaagaggatt ataagaaagt
     2221 cctttactta gattcagatg tgttagtact agacgatatc gtaaaactat atgatgagcc
     2281 gttaaatgga aaaacaatcg gcgctattat cgatccaggc caagtgaaag ctttggaaag
     2341 attaggaatc gattccgatg acttgtactt caattcagga gtgatggtga ttgacattga
     2401 tcaatggaat aaaaaagaaa ttacagaaaa aactatccat tacctcagtg aaaatggcga
     2461 tcgaatcatc tatcacgacc aagatgcttt gaatgctgta ctttatgaag attgggaaca
     2521 acttcatcca aaatggaaca tgcagacttc gctgattttt gaacgtcatc cagcaccaaa
     2581 tgaaaaatat gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg ttcactttac
     2641 aggacatgat aaaccttgga atacgttgaa agatcatccg tataccaatt tatacttaaa
     2701 aaaattagca cacagtactc taacgaaagt aggcgagatc aatgaataaa aaagaaatag
     2761 cagtagtcgc aagttgtaac acaaaattcg taccacattt agctgcatta tttgtctcag
     2821 tattagataa ttgcaacccg tctaaatttg ttcggttcta tgtgattgac gatgatatcg
     2881 attttgaaag taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg aactcagatg
     2941 tggaattctt gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc gatcgtattc
     3001 cagaaacagc ttattatcgt attgcgattc cggaattgtt ccgcggcaca gaagttgaac
     3061 gtatcttata tatggattgc gacatgatcg ctttacaaga tatctctaaa ttatggcgtc
     3121 ttgacttcgg tgattcaatc gtagcagctg ttgaagacgc tgggttccat caacgactgg
     3181 aaaaaatgaa aatttcagct aaatcaatgc gttatttcaa ctcaggtttg atgctgatca
     3241 atgtgaaaaa atggttagat gaaaatatta cgcaaaaagt attagatttc atcgaacata
     3301 atccagaaaa attacgattc catgaccaag atgcattaaa tgctatctta catgaccgtt
     3361 ggttaccact tcatccgcgt tggaatgcac aaggctatat catggcaaaa gcaaaaaaac
     3421 atccaacagc agcaggtgaa cgtgaatacg aagaaactcg caacaatcct tacattatcc
     3481 atttttcagg acatgtgaaa ccgtggagca aggattttga agggccaacg aagaaatatt
     3541 acgaaaaata tgcagggatg acggctttcc gttgtgtagc taaattccct aaatatccaa
     3601 aatatgcaaa agttcaacca aaaacagatg tagttgttaa ataatagaag acaacaactg
     3661 aagaggggga gtaggaatgg aaattacaat gtctaaaaat gcagtagaga cgttaattga
     3721 aaaggttggg gaaaatacaa aaatcgcact cgcgcttatc aatgattccg atccatttct
     3781 aagggacaaa ggtgcttttg caaaaggcag cttttttcaa atcattcctt ttgtttcgga
     3841 atttggagaa tatgcgacta aaatcgagca tccattattg gacatctata catctaaatt
     3901 agagcaaaat tatttcggta aacgattgaa catggacttc aacaaacagc ttgacagctt
     3961 ttcattagaa aatgaaatcg cagtactcga ttataatatc aaattaaaga actgcttctt
     4021 cagctaatag tttcgagaaa gtccaaaaag gaggtataca ttgagatact actttgtgga
     4081 tgaagaaaag aatgtaatag aaacagatga aaaaaaatat aattggctcg tacttgatag
     4141 tgcaaagccc gaagagattg agcaagcaat cgaagaattc gagcttcctg atgatatttt
     4201 cgtaggaacc gaatatccgg aagaagtttc tcggatggag cgcctttatg agacgaaact
     4261 agagcatcca ttttctttgg tagtgattaa tctagatcag tcagataatc gtatcgaaaa
     4321 aaaattaacg ccaatatctt ttgtactttc cgataatctc ttgattactt gtatcgatag
     4381 aaaaaccgaa tttgtggatc ggctgctgac aaagcatgga aatcatttaa actcgtttga
     4441 aaagatcatc acgtatacat tattagatat ttacacccat tacgtgaaag aactaagaga
     4501 gatgaaaaag cgaattgacg ctttggataa agcagctcgt aaaacaacgg aaaatgaaga
     4561 actgtataaa caggcagatc ttgaaagaga catcgtatat atcgatcata ccttaagaga
     4621 tcaacgggaa acaatggatc aattatggaa ttctgaaact ttcatcaaac gtttagacga
     4681 tgaacagcta ctctatgatg tccatttgag acagcgacaa acagaaaaaa tgatcgagat
     4741 ctatcgagat ttattagaga gtattggtga tctattcaac gggatgatgg acaataatct
     4801 aaatcatatc atgaaatatc tagattcagc tgcattaatc atttccgttc cggcgttgtt
     4861 cgcagggatt tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt
     4921 tatactcgta gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg
     4981 gaaagattat acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgttt
     5041 tggcaataca atttaaaaac taggaggaat acagcaatga cagaaccaaa attaatcgac
     5101 ccgcgaaact tgtaccatac ggagaaattt cctaaacaag atcaggatac accagcttta
     5161 cagcagaata tgattcctgt tcctgattgt ggagaagaat cctatgaagg caataatcaa
     5221 ctagaaaacc gccgtgtact aatcactggt ggagattcag ggatcggtcg tgcagcagcg
     5281 attgcatttg ctagagaagg ggcagatatc gctcttcaat tcttcccagg agaagaaaaa
     5341 gatgcagagg aagtagcaaa atacgtgaaa gttgcaggac gtaagattgt cttactacct
     5401 tttgatcttc gtgacgaaca agcacctaaa gaaatcatag ataaagccgt gagtgaactt
     5461 ggcgggctag atacgatggt actaaacgca ggacagcaaa tttcttgtca gtctattgct
     5521 gagttaccac ttgagcaagt caaagacaca tttatgataa acatcatcgc tatgtttgct
     5581 ttagtgaaag aagctgttcc gcatatccca gcaggaggag caatcgtaac aacaacttct
     5641 gttcaagcat tcaatccaag cgaacattta ttagattacg cagcaacaaa agcttcaatc
     5701 gctaacttta cgatcggttt agcaaaacag cttgcaccta aaggaatccg tgtaaatggt
     5761 gtagcacctg gtccaatctg gacaccatta caactagacg aaggacaacc ttctgattcg
     5821 atccctgaat ttggtcagca aagtttacta gaacgtgcag gtcagccagg agaattagca
     5881 ccagtttatg ttttcttagc atcgaataaa gcaagttatg ttacagccca aatttacgga
     5941 gtaactggtg gagaagcgat caacttataa gaagttgatc gtttcacccc tatgaggagg
     6001 tgcgaaaatg aataaaggtg taaaaacgat cggagtgatc ggaacattac ttttgctatc
     6061 tgtgcttttg tttgcgatac tattaagtgc aacctatcca ttgccttttc agctagagaa
     6121 gtttcgattc ttttccatca ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt
     6181 tgcatttgct attttgacaa ttatcacgtt acttgttttc ctgttttatc caaaaacaat
     6241 cggaaacttt ctactgaaac atggtgatgg ggagttgacg ttggataaaa aagcaattga
     6301 aggaatggtt cgttctcact tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt
     6361 caaagcaacg aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc
     6421 actcattggt aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac aacacatatt
     6481 gggaacaaaa gagccagtga aagtatcggt gaaatacaca ggctttgaag aaccagctaa
     6541 acgaagcaat gaccgcaaac gagtagaata gggggggacc aaaaatgaaa gaactatttt
     6601 cgacatacaa gctccctatc atctttgggg tacttggatt gatattagcc gttttatgga
     6661 tcaccgtggg ctttttcaag accttattga tcttgatcat ggcagcgatc ggcgtcgcta
     6721 tcggttttta tctgaaacaa acgaaaatct tagatcacta ttttaaatct taggaggaaa
     6781 catccatgaa taacaataac aaaaataata acaataaaat gaacaaccaa aaaccagtgt
     6841 ctgcacatcc agacccaacg acaccaaacc aatcaaatgg tgtgaacgat gaaaagaaca
     6901 aaacagcacc agtatctgca catccagatc cagtagtccc taacgttcaa caaggagtag
     6961 aagagaagaa acatgatgca gaaaagcatg cacctcatca tccaggaact ccagaatcta
     7021 aagttccaga acaacatgat gcaatcgatc cttctaaacc tgtggaagag caaaaacatc
     7081 cttctgacca tgcgaaagca caagaaaaac ttagttctga ccacacaaac ggacaaggaa
     7141 aacttggttc tgatcataca aataaccaag gaaagcatga ttctgaacag gaagattcaa
     7201 tgcgcggtga gctgaattat gaagataaag ttgtgcaaaa aatcatcgga atcgcaattg
     7261 aacaagtaga cggtttatta tctgcaaacg gtggtttctt ctctaatgtg gcaggaaaac
     7321 ttgtgaatac agataatgta acggctggga tcgaaacaga agtcggtaaa aaacaagtag
     7381 cagttgatat ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc tttgaagaaa
     7441 tgcaagaagt aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg atcgaagtca
     7501 acgctaacgt agtggacatc aaaacaaaag aagaatttga aaaagatcaa gaaacggtgc
     7561 aagacaaagt aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat caaacagata
     7621 aagtaaaatc agcagctgga agcggcgcac aaaaagtaaa agaaaataca gaaccacggg
     7681 ttcagtaata aggaagtaaa acattaggag gaacgaaaca tgggatttat ttggtcactt
     7741 atcgtcggag gaattatcgg agcaattgca ggagcaatca cgaataaagg ttcttcgatg
     7801 gggattattg caaatgtcgt agcaggttta gttggatcag cacttggtca agcattatta
     7861 ggttcttggg gaccaagtat tgcaggaatg gcaatcgtgc catcattgat tggtgccatc
     7921 attttagtac taatcgtatc attctttgct cgaaaactgt aaaaagactt ttaaaaagag
     7981 gtcgtgaaaa agctgttct
//
LOCUS       DACENJ010000119         7947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.122, whole genome shotgun sequence.
ACCESSION   DACENJ010000119 DACENJ010000000
VERSION     DACENJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.122"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            71..2164
                     /locus_tag="IW748_001862"
     CDS             71..2164
                     /locus_tag="IW748_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1104771.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            2324..3190
                     /locus_tag="IW748_001863"
     CDS             2324..3190
                     /locus_tag="IW748_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1104772.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(3212..3649)
                     /gene="ndk"
                     /locus_tag="IW748_001864"
     CDS             complement(3212..3649)
                     /gene="ndk"
                     /locus_tag="IW748_001864"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1104773.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            4308..6986
                     /gene="mgtA"
                     /locus_tag="IW748_001865"
     CDS             4308..6986
                     /gene="mgtA"
                     /locus_tag="IW748_001865"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1104774.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            7073..7744
                     /locus_tag="IW748_001866"
     CDS             7073..7744
                     /locus_tag="IW748_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1104775.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
BASE COUNT         2613 a         1344 c         1674 g         2316 t
ORIGIN      
        1 acttttttag gaaattttca tttttcagga gcaaaataag aaaaaatgtg aatagagaga
       61 agtgatatac gtgacacaac ttaaaaaatt gtgccaaaat cgtttagcaa tcgttttgac
      121 agctgttttc tttttctggc tgaagacgat cattgcctac tatgcagatt tttcattagg
      181 tgtggaaggt acgatccagt actttatctt gtggatcaac ccaattgcga ctaccttatt
      241 gttttttggc ctttctttgt atattaaaaa gccaaagcca gcgttagcta ttcttttgat
      301 cattgatatt ttaaatacgc ttttgttgta cttgaatatt attttttatc gggaattcac
      361 agatttcatt acagtcaaat ctgtattagg cttttcgaaa gtgtcgcaag gattgtcagg
      421 aagctccttt tctttgatga agccccatga tgtcatctac tggctggata tcgctgtgtt
      481 cattgggcta ctggtttggt tgaaggtcaa gaaaatacct atcaaaagca accctgtcgg
      541 caaaccgatg gctatcgctg ttacctgtct ttcagggctt attttttcag gaaatctagc
      601 gctgagcgaa gccaaccgtc cacaattgct gcagcgaaca tttgaccgtt catatattgt
      661 caaatatcta ggaatcgatg cgtatactat ttatgacgga atcaaaacag gaatgactag
      721 cagtgttcgc gcgcatgcaa gcagtaatgg gattgatgaa gttttagatt atacaaagaa
      781 acattatgca gagccaaatc ccgaaacatt tgggatcgct aaagggaaaa atgtgatcgt
      841 tctccattta gaaagtttcc agcagttttt gattaatatg aaggtagacg gacaagaagt
      901 cacaccgttt ttaaatagta ttttccagaa tcaagcaacg atcagctttg acaatttctt
      961 tcatgaagta ggacagggaa agacaagtga tgctgaaaat atgttggaaa ctggcacatt
     1021 tggtttacct caaggctcat tattcacaga acttgggtca gacaatgtct ttcaagccgc
     1081 accagcgatc cttggacaaa agcaaggcta tacaagcgct gttttccatg ggaatgtagc
     1141 tagtttttgg aatagagatc atgtatataa aaacttaggt tatgacaatt tctttgatcg
     1201 atcttatttt gatgaatccg acgaaacgtt aggttatggg atattagata aagatttatt
     1261 cagagaatct gcccaatatc ttgagcatct acaacagccg ttttacacga aatttctttc
     1321 cgtaaccaac cacacgcctt attatacgga tgacaagaat ttcgactttc catctttgaa
     1381 tactggcaat agcacagtag ataattatgt tcgaacggca cattatctgg atcaatcatt
     1441 ggaacaattt tttacgtatt tgaaaaaatc tggtatttac caaaattcaa tctttgtgat
     1501 ttacggggat cattttggga tctcgaatac agataataaa gatttagcaa gcgctcttgg
     1561 taaagatcca gatacatggg atgaatttga caacgcacag atgcaacgag tgccattgat
     1621 gatccatatg cctggttata ctaaagggac agtcaatcat gaatacggtg gagaaatcga
     1681 tgttttgccg acattactac atctattggg aatcgatgat aaagattatc ttcattttgg
     1741 tacagactta ttatcttctc agcatgatca agtagttgct tttcgaaatg ggaactttgt
     1801 gacacccaaa tacaccgtcc taggaggaaa agcttacaat aatcagacag gagaaatcat
     1861 cgatacgaaa gcagcaggaa tcgatgagga aatctccaaa gatcaggaaa aagtcaagac
     1921 tgcgttaagt ctttcagata agctgaatca ggaaaatctc ttgcggtttt atgtgccaaa
     1981 cggatttgag acgattgatc cgaaaaaata tgactataaa aatgaagcgg aacgtaatga
     2041 agcaatcgaa aagcaaaaag gcgaacattc gacaagtgtc ttttcaaaaa atggtaataa
     2101 aacaacaacc aacttatatc cagtaacaag aacagacgtt gaacagcaaa aaaataatca
     2161 ataaacaatc aaaccaacct tcagtagtta gacaacattc taatgcttga aggttggtat
     2221 tttttatgaa aaaagcattt ttttattttt ttcgtgtatt tttccttttt aacgtatcta
     2281 tttattgaat gtggaaaaat ttaaattaaa aaggagaact attatgtatg atggtgaatt
     2341 gatcaagaaa cttcgagtca acaagaagtt gacacagtcc caattagcgg aaggaatctg
     2401 ttttaagact tctctagtcg ggattgagac aaattcagtg aaaaaaatgt catttgtaac
     2461 attgagatca tttttagagc gaatgaacat gacacttgca gaatatgaat ggatgcgaaa
     2521 ccagctggat gaaccaataa agttaaagaa aaaaagacga atgctagatc gggtacaaga
     2581 agacagcttt gatccttata aagagatcag caataaccgt aggcatttta agaaaacaga
     2641 agatctgtat tatttggttt tgaatcttca catggtcgaa aaaacagaag tatctgagac
     2701 agaatttgaa tttttacgtt atgaatgccg aatcattgaa gaatacttga tgcagatacc
     2761 agactatggt caatttgaac tatctattct agctgatttt ccttggatat tcagtgatcg
     2821 gttcattgag aacaattatt tgaaaatcaa aaaaagaatg cgacgattag cacagttgaa
     2881 caatcatgag cagtatctct ttactttttt aatgaacctg acttcgtttt atatagaaaa
     2941 tggtcggttg aaaaaggcac gtagtgtgaa tgaagatatg aaacggagct tagcacataa
     3001 agaaagaagt acagtgatct atgaaacatt gatggcagat tattatcaaa gattgatttc
     3061 tgctgcttta ggggaagaaa agacgaaaga ctaccaagaa ttcttcacag tgctcgaata
     3121 tatgttagga aagaatcaac ggaggtcctt agagaaaaaa ctgctggcca ttgctgaacc
     3181 cagcaactag accagcagtt tagaatttgt atcacttcgc cgctttttca gacggtttgt
     3241 ttatatcgaa tggcgggaaa aagcgggcga tctcttttac cgcagcatca cgggaatcag
     3301 aagcgtgaat gatgttttca gtccccggca gcgcgtagtc tcctcggatc gtacctggaa
     3361 cagcatcggc agcttttgtt gctccaacca tcttacggac gatatcgatc acttcctcgc
     3421 cgaccagcac aagataaacg acaggaccgg aagtcatgta atcgatcagt tcatcaaaaa
     3481 aactgcgctc agtcaagtgc tgataatgtt cttttgctaa ttcaggagtc atagtttcaa
     3541 acttcatttc agcaatggcc agtccctttg cttcaaaacg ttggatgatg cttccgacta
     3601 aatggcgtct cacgccgtct ggcttgataa taacaagtgt tctttccata acagcacctc
     3661 gtttcctact atatttctat tatagcggga ggtggaaaat gagacaaata aaaccgcgtg
     3721 agacaggaat ataacgtgtg tattttaaaa aaagtatgaa aaacatggaa aattgatttg
     3781 acatcctctt ttttagttgt tatattaacg aaggaaaacc gaataagatt tgttaggtga
     3841 ggctcctata caaacatagg ctactgccca aaaacgtcga gagacgccaa tgggtagaac
     3901 aggaattgtc gagataaggc ttttcataag gtagctaaaa gcaggtatct gctttttacg
     3961 ttgtacagtg tcaaaactca acgatgagat ctgagaagct ttttttaata tgctttttca
     4021 aacctttcgc tgagtatttg gcgagaggtt tttttgttgc cgcaaatccc catcggtgaa
     4081 aaataaaaag gaggtggtct aattggaaaa ataactattc gaaaaatgat tggaagtcat
     4141 gaattaaaat tacttagtaa aggagatgag aacaaatgga tgacagtcat tcgaaaaaca
     4201 aggatctaga ggaaaggcca gggctattat ctatctgttc tcagcccctt tcagtttttt
     4261 ctctatcgat ccgatgaata gctaaagacc aaaagcagcg agaacagctg tcttttggac
     4321 acaaaggaga gattgagatg gaaaaaaaga aagatacaca aaaaatctat gcagcttttg
     4381 ctggaaaaac gaaacaagaa ttatttgaac tattccatgc atcagcaaat ggattgacag
     4441 aagaacaaat cgaacgttca agagaaaatt acggtgaaaa tgcgatttca tatggcaaaa
     4501 aaacaccatt cattgtagaa atattgaaag catacatcac gccatttaca ttagtgttga
     4561 tcgcattagg aattatttca ttcatcacgg aatatgtatt agcagctcct ggagataaag
     4621 atcttttcgg tgtagccatt atcttcacta tggttatcgt cagcgggaca atgacgttga
     4681 ttcagtccgt acgttccaat caagcggctg aaaaactgaa atcacttgtg aaagtaacgg
     4741 cagctgtaaa acgtaatgga aagtacacag agattcctat ggaagaaatc gtctgtggag
     4801 atcttgttcg gctttcagca ggggatatga taccagcgga tatgcgttta ttgtccgcaa
     4861 aggatttatt catttcccaa gcagcattga caggggaaag ctatccagta gaaaagcatg
     4921 cgaaagtatg tgaagacgat cagcaaagcg aaacaagtta cgaaaatctt gtctttatgg
     4981 gaagtaatgt catcagtgga agtgcggaag gaatcattgt ttctgttgga aatgaaacac
     5041 tatttggtca tgtagcagaa acgctatctg aaaaaccaat tcaatcaagc tttgaattag
     5101 gcatccataa aacatccatg ctactgatca aatttatgtc attgatggct ccagcagtta
     5161 tcgtcatcaa tgggcttaca aaaggggatt ggctagaagc ttttcttttt ggactatctg
     5221 tagcagttgg tctaacacct gaaatgcttc caatgatcgt tacgacgaat ttggtcaaag
     5281 gagcaagtgt gatggccaaa aaaggaacag tgatcaaaaa tttgaatgcc atccagaact
     5341 ttggtgcaat cgatgtgtta tgtacggata aaacggggac attgacacaa gataaaatca
     5401 ttcttgaata tcatatggat tgtgaaggaa atgaagatga tcgtgttttg cgccatgcgt
     5461 atctaaatag ctactatcaa acagggctga aaaatttgtt agatgtggct atcattgatg
     5521 aagcaacaga aactttagat acagataaga tcaattatca aaaagtggat gaaattcctt
     5581 ttgattttga acgtcgtcgt atgagtgttg tggttgctga tccagctggt aaaacacaaa
     5641 tgatcacaaa aggcgcgatt gaagaaatgc tgaacatttc taaatttatt gatcttggcg
     5701 gtactgttac accattaaca aaagaacgaa aagaagccgt acttgcaaaa gtacagtcac
     5761 ttaatgaaga tggcttacga gtgattggtg tcgcacaaaa gaccaatcca agcgtcgttg
     5821 gagaattttc agtcaaagat gagtcagaca tggttttgat tggttatctt gctttccttg
     5881 atccaccaaa agaaacaacg aaacaagcgt tgaaagctct gaaagaacat ggtgtagctg
     5941 taaaagttct gactggggac aacgaattag ttacacgttc agtgtgccgt caagtaggtc
     6001 tagagatcaa tgaattggtt accggtgaga aaatctcgga aatgagtgat catgaattag
     6061 cacaagtagc tgaagaccat gaagtattcg taaaattgaa tcctcagcaa aaagcacgtt
     6121 tgacgacagc tttacgtaaa aatggtcata ctgtcggatt tttaggagat ggtatcaatg
     6181 acgctcctgc aatgaaagtg gctgatatcg gtatttctgt agatacagct gtggatattg
     6241 cgaaagaatc tgcagacgtg atccttttag aaaaagattt gacgattttg gaaagaggcc
     6301 ttctttctgg acgagaaact tttgggaata tcatgaaata tatcaaggct acagcaagtt
     6361 ctaattttgg caatatgttt tccgtgctga ttgcaagtac gttcctgcca tttttaccaa
     6421 tgctgccttt acaacttttg tttttgaatc tgatctatga tgtttcctgc atttccttgc
     6481 catgggatcg gatggataaa gaatatttgg acgagccaaa aaaatggaat gcatctagta
     6541 ttggggaatt catggtttac tttggtccaa caagttcgat atttgatatt actacttact
     6601 tgttgatgta ttttgttatt tgtcctgctg tagtgggcgg aagtttccat acattggata
     6661 gttcacagcg agtgattttc atcgcattat tccatgcagg atggtttgtc gaatcactat
     6721 ggacacaaac cttagtgctc catgcactgc gtacaccgaa agtaccattt atccaaagca
     6781 acgcaacatt tgccatgttt gttatcacta cattaggaat tattgtcggt tctgtccttc
     6841 cttttacaag ttttggtgct gaattaggct tgatgccact tcctggcaat tactggttat
     6901 ggttatttgc tacagtcatc gcttatctgg cgcttgtgac agctgtgaag aaaatctata
     6961 tccgtcgatt ccaagaactc ttataaacga gaaggctggt tctttgcttc tgttcttttt
     7021 ggaccagtcg tgctaaacta tgtgtattgt tttggaaagg attaatgtat agatgaaagg
     7081 tgcaatattt gatttagatg gtttgttagt tgacacagaa aaaacgtatc gtgatggctg
     7141 gctttgggga tttcagcaat ttgatatcca tatcccaaaa gaaatcgttg acgcttgggg
     7201 cggaaaaaac tggaaacaaa gttttgatat tttagtgaaa gcagcaggta gccctgaaaa
     7261 agtacaggaa gtccgagcga agcgtgaaga atatttttat caacagctga caagcggagc
     7321 catccactta aaaccatatg caaaagaaat attggcagac ttaaaagaac gtcatatgaa
     7381 actaggcatt gctacgacca ctgttacgaa aagagcaaca gatatcttgg atcactttaa
     7441 attagctgat tacttggaca cgtttacatt cggagacgaa gtgtcggaga acaagccttc
     7501 accgattcct tatctgacag ctttggaacg aacaggatta gaagctagtt cagcctttgc
     7561 agtggaagat tctttagtag gagcaacagc tgcttcacga gcaggattag gcgttgtttt
     7621 gatacctgat tccagttttg tccgaaatta taccgtcgaa gaaaaggaaa aattgaaact
     7681 tcttgcggaa ggaaaagatc ttcgaactgt tattgaaatg ttaaataaaa aaacagaaaa
     7741 ataaatatag aaaaaataat ttgaatttcg tacttgactt ttcagttttc ctcatgtaga
     7801 ttaatcccaa caacaaaata tcgatttcgt tgaaaaggaa agtaagtttg gaaactttgc
     7861 agagaggctc tggctggtga gaagagtcaa gtaagaaaag ctgaaaatgg ccttggagaa
     7921 taatttgtcg ataagtaggc aaattcg
//
LOCUS       DACENJ010000120         7920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.123, whole genome shotgun sequence.
ACCESSION   DACENJ010000120 DACENJ010000000
VERSION     DACENJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.123"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(25..573)
                     /gene="aac(6')"
                     /locus_tag="IW748_001867"
     CDS             complement(25..573)
                     /gene="aac(6')"
                     /locus_tag="IW748_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1104776.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1126..2229
                     /locus_tag="IW748_001868"
     CDS             1126..2229
                     /locus_tag="IW748_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1104777.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2422..3489)
                     /gene="rlmN"
                     /locus_tag="IW748_001869"
     CDS             complement(2422..3489)
                     /gene="rlmN"
                     /locus_tag="IW748_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1104778.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3719..6166)
                     /locus_tag="IW748_001870"
     CDS             complement(3719..6166)
                     /locus_tag="IW748_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1104779.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6355..7791)
                     /locus_tag="IW748_001871"
     CDS             complement(6355..7791)
                     /locus_tag="IW748_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1104780.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2197 a         1723 c         1355 g         2645 t
ORIGIN      
        1 tacttctgtc acaacctctt tttattattg agaatttggt cgaggaataa tcgtttttgc
       61 catccaaata tccggtttgt cccagccatt tgcatttggt aagacaccta cgattttata
      121 acctaatttt tcatagaatt catacggatg ttcacgaagg ttctggatgg aagccacttt
      181 atcaaatgta tgctcataca ggtccgtttg acttaacgtt gttccatggt ctaaatcatc
      241 cgtacctaaa taaatcgtga ttcctcctct ggaagctact tctttttcta agtaattgac
      301 taatcgagta cctatttggt tctttcgtcg ggagctttct acaactaatg gatgcaattc
      361 ccaacctgtg ataccgtatt gagggattgc accaataaat cctactaact catcttggtc
      421 taccgctgct accgcgattc gttctggatt catcatttct tctacttctt ctgccgagct
      481 gtctccatat tcttccggcc aagtcagtct cagtaaatca gaaagctgat ctttcaatac
      541 tggattatta cggtcaaatt cactgattat catacgtctt cccccttgtt cttgcttatt
      601 ctaacagtcc ttttgaaaaa acgaaatgtt ctcgctcaaa aatctttagc taccaatcag
      661 tatctctatt ctttcattat ttatcttact tataaaataa aaaaattata cagaaacact
      721 ttattcaaaa taggaaagat caggaaagcc atcaattgga ggaaaaaact ctgtttgttc
      781 atcatgatat agattatctt ttgctttaaa aaaaaccaat tatgaattga caaataaaac
      841 ctgttttcat ataataatct taatttttag aatttttaga taattacaaa aaagcaaagg
      901 gaatcggtag aaatactaag aggaacaaaa attcattatt gtcgttttca atgtttttgt
      961 acttgttcgt ttttaggata cttattgatg atttcaaggt gttcatgcca ccttattcat
     1021 cacttatcct gtcttaggtt cacctctact ccatctacag ccgcagtcag atcgatcact
     1081 ctttaaaatt ttaacccgtt tcaacgaaga aagaaggaaa tgaatatgag tcaaagtgtt
     1141 ttcttaccaa attataccat tggaacagat gcgtatgaaa aagcaaatgg aatcgtcaaa
     1201 gaatatggga caaaagtagt gctgattgga gggaagactg cactttcaaa ggcagaaaac
     1261 aaaataaaag tagctttgac acaggaaata gaggttgtcg ccagtctctg gtacgggggt
     1321 aattcaactt atggaaatat tgaacagttg atccagcatg aaagtgtcca aaaagcagac
     1381 gtcctgtttg cagtcggtgg tggccgtgct tgcgatactg taaaagtcat tggtgaaaaa
     1441 ctagctaaac cagtcttcac ctttcccact attggttcga actgttcgcc cgtcactgcg
     1501 gtatgtgtca tctataacga agaaggcgta atggacggac tcttctttcc ttcaaaacca
     1561 cctgtacaca ccttcattga tacagcaatt attgccgaag cacctgataa atacttatgg
     1621 gcaggtattg gcgatgcttt atccaaagaa gtcgaatcta ctttttctgc tcgtggcgat
     1681 gatctaggct acacggatag attaggaatc caaatcggaa aaatgtgcat tgatcgtctc
     1741 ttgacttatg gtacgcaagc attgaaagat agtgccgacg atcgtccctc atatgcaatt
     1801 gaacaagtcg tttcggatat cattgttact accggattgg tttctgtttt agttaacaat
     1861 aactataatt ctgctatggc acactcattc tattacggta cgactgttct agaaaaaatg
     1921 gagggatacc ttcacggagc atctgtcagt tatggtgtac tgaccttatt gatgatggat
     1981 cagcagttcg atttgtttga taaaatctat gtgtttatga aaaagaacca acttccagta
     2041 tcgttaaccg acatgaatct caatgtcacg accgatttag aagcaatctt agataaagcc
     2101 atggcaacaa atgatattaa acatgtgcct tatgaaatca cccgagaaaa atttaaacag
     2161 gccattcttg acgtagaaac ttatcatgct acacaagaaa gccagcaagg atataagaaa
     2221 atcagttaaa taattgctaa aacccccatc aagcaggctg tcatcaaatt gaggacattt
     2281 ctttgcttga tgagggtttt tcttgtcaaa aaaagcttta tagctttcgc tatagaatct
     2341 tttctcttat ttaagttgaa cagttcctta attaagaaaa ctgctccttt tatggtaatt
     2401 gaaaaaacga gtaggatacg attatttggc aactgttttt ttcatttgct tgcttcgcaa
     2461 ttgtccgcaa gctgcatcaa tatctgtacc atgttcttta cgtatcacac agtttacgcc
     2521 gtttttcttc aatacatcat aaaatctcaa tacatcagct tttggactgc gagaatattg
     2581 atcatgttca ctgactggat tatatggaat caagttgaca tatgctaatt ttttcttatc
     2641 ttttaataga tctgctaatt gttgcgcatg ctctggtcta tcatttactt gattcaacat
     2701 gatatattca aacgtgattc gacggtttgt tttctctaaa tactcatcca cagcttccat
     2761 caatttctca attgggaaac ttcggttgat ccgcatgatc gatgttcgca cctcattatt
     2821 cggcgcgtgc aaggaaatag ccagattgac ttgcaagccg ttattagcga attccttgat
     2881 tttgtgtgct aatccactag tggaaactgt aatgtgccga gcaccaattg ccaatccttt
     2941 ggcatcattg atgatatgca aaaagtccat tacattgtcg taattatcaa atggctcacc
     3001 gatccccatt acaacgacat gtgaaacgcg ttcgccaaga ttgcgctcat caaaataatg
     3061 ttggaccatc atgatctgtg caacaatttc tccagcagtc agatcacggt tctttttgag
     3121 cagcccgctt gcacaaaacg tacagccgat attacatcct acttgtgtcg tcacacaaac
     3181 tgacaagcca tattcttgtc tcatcagtac agtctcaatc atgtttttat cagggagttc
     3241 aaacaaatat tttactgtac catccgaagc ttcttggacg accatctgtt ttagaggatt
     3301 gatgataaaa tgctccgata atttttcgat caattgtttc gatagattcg tcatttctgt
     3361 gaaattactt acacgttttt gatacaacca ttcccaaact tgggcagctc ggaatttttt
     3421 ctcgccgttt tctataaacc aattgattaa ttcttcattc gtcaagccat aaatggactg
     3481 tttttccaaa gtattttccc tctttttctc aaagtttcgc tacatactat acatgattca
     3541 tttccttaat gcaatacttc acaaataaat cgaatagaac tttaatctca caaaaactaa
     3601 aaagacaaga catcataccg tattagaaaa gatggaaaaa aaccattttt tcttttggta
     3661 tcatcatgcc ttgtcttttt attggttatt cgtttttcaa cccttcatca cacaagtttt
     3721 acatgtcatt atcgtccggc aacgtttctg atgtttcttc ctgctcaatg aatactttcc
     3781 gaggcttgct tccttctgac gggccaacaa ctccatgagc ttccagttca tctactaaac
     3841 gtgctgctcg gttgtaaccg atccgaaatc ttctttggag aagggaaata cttgccgttt
     3901 gcatttcaac gacaagtgct tttgcttctt caaacagctc atcttgtggc tgttctggag
     3961 cacctgtcgt ttcaacttca tctgtcggca tcattttctc ttcataatga gcttcttgct
     4021 gatctgtaac aaattgaacg acccgttcta cttcgtggtc agaaatgaat gctccttgca
     4081 cacggatcgg tttattctca cccattggca agaatagcat atctcctcgc cctaagagct
     4141 tttctgcccc attagaatca atgatcgtcc gagaatctgt accgcttgat accgcaaatg
     4201 ccattctgga aggtacattt gctttgatga ttcctgtgat aacatccaca cttggacgtt
     4261 gagtagctag gatcatatga atccccgctg cccgagccat ttgcgctaaa cgaataatcg
     4321 catcttcaac ttcattgctg gctaccatca tcaaatcagc taattcatct acgatcacta
     4381 cgatgaatgg taaggttgga tgtttcgttc cgttttccaa gtttttctgt tgaacaaatt
     4441 cattgtaacc agaaatattt cttactccgg ttgccgcgaa tttttcatat cgttcttcca
     4501 tttcttgtac tactttttgt aaggcttgcg ctgcttttct agggttcgtc acgaccggcg
     4561 tcaacagatg aggtatgccg ttgtacatat tcaattcaac cattttcgga tcgatcatca
     4621 tcagttttac ttcatggggc ttagcacgca ttaatatact tgtgataatc ccattaattg
     4681 ctactgactt cccactacca gtcgaaccag ccacgagtaa gtgaggcatc ttcgaaagat
     4741 cagccgtttg tacccgtcca gaaacatcac gtcctaaagg tacttctagt aatttatctg
     4801 ggtggtttgg ttgtgcttcg atgatttctc taaaggaaac catactgatc tttccatttg
     4861 gtacttcaat accaatcagt gatttacctg gaatcggtgc ttccatccgg acatctttcg
     4921 ccgctaaagc aagcgcgata tcatctgtta agttcacaat tttacttact tttaccccta
     4981 cagccggctg gacttcgaat tttgtgacgg atggtcctaa gctggctttg acgaccttgg
     5041 catcgacacc gaaactttgg aaagtttgtt ctaatacgcc gatatttttt tcaatttttt
     5101 tatactcatc actttgatcc acagttggta tagaatcaag caaatcaact gtcggtaatt
     5161 catagtcttg attttcctgt tctgcttcaa tctcaaattc taatgaggta ccgtcatcct
     5221 ctggcaactc atctgaaaaa ccttctgctg tattggtctg ttttggtgta tctggtactg
     5281 gtttctctag attcgcagga tggatatttt cttgaaaact gtcgattgga acaaaactta
     5341 gctgctccgg ctctgtattt tctggttcct gccattcatc agcagtattt tccctttgct
     5401 ggctcaccgc ttttttcttc tcatattctg cagcttcttt ttctgcagct tcttttgcag
     5461 ctaagcgcct tgcttctttc gcttctgcgc gctgtttcat tctctcttcc tttgccgctt
     5521 gctttcgagc ttgcttggca ggatcacctt ccaataattc ctgtactcgt tctccaattg
     5581 attggatacc atttaacagc tgatagcctt ccagattact gaacaggaat gctcctcctg
     5641 ccagtaatag cactgctgct aaataacttc caggctgtgc aatcaagaag taagtaaaat
     5701 gataaaggat tgctccaatc atcccaccac caacgttatt gtctacttga ctttgtttta
     5761 tatcactggc taacaaatcc catgtcgtcc ccatgatatt tggttcccca gtatggagtt
     5821 tgccaaataa atgggcatgt aacaataata agataccgaa ataaaagagt atgccgccaa
     5881 accagcgtcg gtttttacca attgaaaact ctgtattctt aatgacaatc caaaatccta
     5941 atacggctaa taaaagacag aggatttggt aagtatttcc tgcaatgatc cgaaagccgt
     6001 ttgcaatcaa tattcctaaa aaacctaatt ttaaaaagcc gaagacagca aataaaatca
     6061 aggcaaaccc aacaccaatc gtcagcattt gttcctgctg ctgtttttgc tttttcgttt
     6121 ttttcgtctt tttccttttt ttagtaggcc gtttcttttg tgccatttat tatccctcat
     6181 ttcatcaagt ttcattatac aaaaaaaagt aagggaacta aagttttgtt tgaaagaact
     6241 gacgctttct ttcatttttc agcttatttt acagtttttc ttaatttcta aatgatttta
     6301 tcaagaaaag gcacggccat atccagccgt accttcttgt tttaagtatg tgaactaaag
     6361 gttctctcca ttttcttcaa tcagtttttg gtaccagtaa taactgtctt tcttatatcg
     6421 ttccaaagat ccttcttgtg tttcgtcttg atcaacatat acaaatccat atcgcttttg
     6481 ataaccgttc agccagctaa gcaaatctgt ataagaccag gtgcaatagc ctaggacttc
     6541 tgctccatcc gaaattgctt ctttgatggc tttgacgtgg ctttgtaagt aattgattcg
     6601 atagtcgtca tggatttgtt tatcttctgt caacttgtca tactctccta agccattttc
     6661 cgtaatcagt accggcatgc ggtaacggct tgtgatacga cgcagtccga tccttaatcc
     6721 ttctgggtca atctcccagt cccaatttgt tcgttcgaca aatggattct caactttttt
     6781 atacattcca ggaactccag tttcttctga tgaacctttt tttcctgtcg tattcatttt
     6841 tccagcacca actccgttta atggattgaa tgcattcgtc gccgtttgat agtaattgat
     6901 cccaagaaaa tctggtttag cagattctag caattctgca tcgccagctt caatcgttgg
     6961 tgcccatcct ttttcttcta agaacctcat ggcagcaatc ggatattttc cccacatata
     7021 gacatccatc caataattgg ccatcaggtc ttctgcattt tcagcagcca gaacattttc
     7081 aggattactg tcaattggat aatttggcgt atacgcaaag cttggtccga tttttccggg
     7141 catttctaac tcgtgaaatt tattgatgac agatgcattt gctaaattag caatatggtt
     7201 aacttcatac attcgtttcg ggtctgtcac acctggcgga tgtgctgcta acaaataacc
     7261 taatgaagtg aagatatttt gttcattcaa actaatccag taatggactt tcccgcggaa
     7321 tgcctcaaat aatgtttctg cataatgagt gaaatcttca atgatctgac gtgattccca
     7381 accattatat tcatcttgca atgcttgtgg aagatcccaa tgatataaag tcaacatagg
     7441 ttcaatatcg ttttcaatca attcgtcgat caatctctca taaaaagcga gcccagcttg
     7501 attgacttct cctcgtccat taggaaaaat ccgtgtccaa gctacagaga agcggtaagc
     7561 tttcagacct tgttctttca ttaaagcaat atcttcttta taacgatgat agtggtctac
     7621 tgccaaatca ccgttcgttc ctttaaatgt ctttccaggt atccgtacaa atcgatccca
     7681 gactgattct cctttcccat cttcttgcca agctccttct acttgatacg ctgcagaagc
     7741 agagccccac agaaaatctt ttggaaatga ggtaagttct ttgtgttcca ttcatctctt
     7801 cctttcctat aaccttcaat cgttcctctt ttagaataac acgaaacccc ttccacaaca
     7861 aaataaaaat atgtaaagga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa
//
LOCUS       DACENJ010000121         7790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.124, whole genome shotgun sequence.
ACCESSION   DACENJ010000121 DACENJ010000000
VERSION     DACENJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.124"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            106..390
                     /locus_tag="IW748_001872"
     CDS             106..390
                     /locus_tag="IW748_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1104781.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            393..1310
                     /locus_tag="IW748_001873"
     CDS             393..1310
                     /locus_tag="IW748_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1104782.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            1303..2085
                     /locus_tag="IW748_001874"
     CDS             1303..2085
                     /locus_tag="IW748_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1104783.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            2086..2766
                     /locus_tag="IW748_001875"
     CDS             2086..2766
                     /locus_tag="IW748_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1104784.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            2759..3655
                     /locus_tag="IW748_001876"
     CDS             2759..3655
                     /locus_tag="IW748_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1104785.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            3850..5499
                     /locus_tag="IW748_001877"
     CDS             3850..5499
                     /locus_tag="IW748_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1104786.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            6093..7028
                     /locus_tag="IW748_001878"
     CDS             6093..7028
                     /locus_tag="IW748_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104787.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            7021..>7790
                     /locus_tag="IW748_001879"
     CDS             7021..>7790
                     /locus_tag="IW748_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1104788.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         2406 a         1350 c         1717 g         2317 t
ORIGIN      
        1 aagaagggat tgtgaaaagt cttttgtcat aacctcttat ttggtagcta attgtaaatc
       61 ccaaacggca tgtattattt ttcataagtc cgtttttaag gattaatgtt ttcttttgct
      121 ttttatatgg tctggcttca gcgagaagtt ccaacgcata tacaaggacg tctgatgtca
      181 acgataacaa cgattaccat ggtggccagt ccacttggat tagcactgtt tggtgcgctc
      241 tttgattggc agacaactca accacgtctg cgagacagtc tgatttttct tgaaattcaa
      301 ttggaaaatt tacactttct ttatacattt ctggctgctt tctttatacc cgtcaactat
      361 acttcactta tcaataagaa agaaggataa gtatggattt tggaaatgaa acggtcttga
      421 aagcaactgg tataaccaaa aaatacggtg ctgccaaagc attagataaa gtatcgatcg
      481 aaatcaaacg tggaatgatc tatggtttga ttggggagaa tggagcgggt aagtctacct
      541 ttatgagaac tattatgggt ttgatctcga ttgatgaagg ttcgatcgaa ttatttggaa
      601 cgacggattt gcaggcagca agacgacgta tgggtcaatc gattgaaacc cctgctcttt
      661 atccagaatt aactgcaaga gataacctac gtatccaagc agcgaacggc ggtgtgtcgg
      721 atagagaaat cgaagacctg ctgaaaatga tgcgattgga aaatacaggg aaaaagaaag
      781 caaagaattt ctctttaggt atgcgccagc gtttagctat tgcaaatgca ctgatcacga
      841 atccagaatt tttgattttg gatgaaccga cgaatgggat ggatccagca gggatggcag
      901 aaatgcgaga aatcatccaa cgtttggtaa aagaacgggg catcaccgtc ttgctttcta
      961 gccacttgct agatgaattg tcacagatcg ccactcacta cggtattttg catgaaggac
     1021 acttgatcaa agaattatcg aaagaagaat tggcacaaga atctcgtcaa tttataaaaa
     1081 ttgacacttc ggcgacggaa caagcagtaa cggttctcga ctctcttggt tatagggatt
     1141 attttgtcca atctagccgt gttatccagc tttttgaagg aattgaccaa gtagcagcca
     1201 tcaatcaagc attggtagag gcaaaagttc ctgtggatgg tatccaccta gttgggcaaa
     1261 aactggaaga ttatttctta caattaactg gaggcaatcc acatgtttaa tctattaact
     1321 gctgaaaaaa taaaagtatg gaagagccgt aaaatgtggg tctcactagg actgatggtc
     1381 gtacttccgc ttttttattc ttggaacgaa tggtatatgc atacaaaata cggcaatgaa
     1441 ttaaaccaag caacggatac agtcatcaac ggtgcaacag ggattttaat ggtggaaaaa
     1501 atggctgcat ggattcttct ggctttcgca gcatttgcgt gcttttatat tggggaagaa
     1561 tttcaaaacg gtacgattcg caatacgtta tctttaggac ggaatcgaat gacttattat
     1621 gtgtcgaaac ttctcgttac gcttttgatc acaacacttg gcgtggtttt gattacgggg
     1681 ctagccatga ttgcttacac acttgctttc ggatttggtg aagttgaggg aatcaaaaac
     1741 tatggaaatt atgttctaaa agtatttcct gtattgcttc tgctgatatt agcgacatta
     1801 tctgttccag tggctttgac attcatcact agaagtacca gtgtcagttt gcttctttct
     1861 tttctttata tcatgggaac ggccttcgtt cccggtgttt tcgcaaaaat caaaggcttg
     1921 gaatttctaa cggagtggtt tacggaaacg tggctcatgt atacggactt tgcgcaacaa
     1981 gcaacttatt cgcaagcacc gaaaatgatt ctggtcagtc tagtaacaat cgtggtatca
     2041 tcagctctag gaatgtttat tttccaaaaa tctgacatta agtaggtgtt cctgctggaa
     2101 actattttga ttatcgaaga cgatgaagct gttcattctt tactggagga ggttcttgaa
     2161 cagagataca aacttcttga tgcttattca ggtacggaag gaaaattatt gttaagcaca
     2221 tatcctgtcg atttgatcct tctagatctc atgcttccag ggctttcagg tgaagcattg
     2281 ttagcagaaa tccgacagac atcgaatgtt ccgatcatcg ttctttcagc aaaaagcgat
     2341 caaagagata aagtttcctt gttggcagca ggagcggatg actatgtgac gaaaccattt
     2401 gatatcgaag aactgttgtt acgaatcggc atccaacttc gtcatcagac ttctgtccaa
     2461 gtaaaagatc tgtcgcaacg catctattac aaggaaatct tactggacaa agagtcgagg
     2521 gatgtaaatg tgaatggtgt ggctgtccat ttgacaggaa gagaattcga tctgctgaag
     2581 ctttttttag aacatccgaa aaaagtattt tcgcgagcaa atctttatga aacagtttgg
     2641 caagaaccat attttgatag tgagaaaaca gtgaatatgc atatttccaa tcttcgaggg
     2701 aaactagcaa aggcacatgc tgtgtatatt catacggtgt ggggagtggg gtttagattt
     2761 gattaagtgg ctgttgcttc tcgttgttat ctttatggga agttttattt tcttcctgca
     2821 aagggaaatc aagagattaa ctaagaaaat cgccactctt tctgaacgac aagaatatgg
     2881 tggtcgtctt tcagtcgaat ttcgagaaaa gaatctaatg gacctagtag atgcactgaa
     2941 tacatgggtg gatgaaaatg aacaaaaagg acaagcattt caagaaatgg aagaaaacat
     3001 cagactatcg atcgtcggcc tttctcatga tctcagaact cctttgactg ctattaacgg
     3061 ttatgtacaa ttactggaac aggtagagga tacggagaaa cgggagaagt atttaacgat
     3121 catcaaacaa tctgttgcaa gattactcag tatgacggat cagttttatg atcttgctcg
     3181 aatagagacg aagcaaaaag aaatggatct ccatacgctt tctttttcta aagaggtgga
     3241 agaagtattt ttctcttttt atgaacaatt ttctgagaaa aatatcgagg tagcgttcgc
     3301 tgaacactcg ataaatacaa ctgtattagc agatccgctc atgttgctgc gagtgctgca
     3361 aaatatcata cagaatttat tgcgttatgc agatggacaa gcaaaggtga cattttcaaa
     3421 agaggattca ttcattcgat taacagtggc caatcctatc aaaccaggag cgcaaacctc
     3481 tgttgaaaaa gtcttccagc gtttttatac cgaaaatacc agccgcacaa atacggaatc
     3541 tagcggaatc gggctttacc tgtcaaaaaa attggtggaa ggaatgggag gagagatgac
     3601 cgctagatta gacgggggca tcttctccat ctccgtcaag ttgcggaggg tttaattggc
     3661 tctaatttac ctgaagtgaa tgacaaaaaa ttgtatctcg ttttaaagac atcttctttt
     3721 ttggaaggtg tttttatttt ggtgtgattg atacgaatta ggtaacgaag acagttattt
     3781 ttgtaccccc ttttcaaata gcgcaacagt gctatactaa aactaacaaa tcaaggaacg
     3841 gagagggata tgggccaaag agaatttatc gttttaatta ttattgcagt tgttatttta
     3901 ttagttgttt tagacatttt ttcacgtttg aaagtaaagg aaacagttcg tagtaattgg
     3961 ggaaaaattc cttatcaacc gagatttgat aaagaagaaa gtttgaagga tgcgtggctc
     4021 acggagaaaa aattccgttc atgggatagt gaaatcgacg atcttacttg gtatgacttg
     4081 gatatgttcg aagtatttga agggatcaac agtacctatt ctagtgtagg ttcagaagcg
     4141 ttgtatcagc gtttacgtag ctttgatttt ggagaagatt atcagttaga aaaactaatt
     4201 gctttttatc aggaaaatcc tcaactgaga gaaagaatcc aatatcaatt cgctcgttta
     4261 ggaaaaaaag atcataattt tgcaaaacaa tatctagcgg atggaaaaag taaaaagatt
     4321 ggacgcgtag gtttatttat ttttcttggc ttgcttccgc tggtgggtgt aggtttgctt
     4381 cttttagggc aagtactagg aatctatcta gcaatcggtg gcttagtatt caatacggtc
     4441 tattatctag tgaagaaaca aacactagaa acagagctga atagcatgag ttatttggtt
     4501 tcaacgattt cagccgccaa tcagttagca aaaatgacga caccacttca aaaagagctg
     4561 aaacagcaaa ctaaaccatt aagagacatc cctaaattag gtttttcatt tcgaatgaag
     4621 agcgggagtg aagcagaatt gatttttgaa tatgtcaaca tcatgttcat gctgccattt
     4681 atctcctatc atttcgttct cactaaatta gaaaagcatt caactgaagc catcaagctg
     4741 tgggaattgc tgggagaatt ggaagttgca gcagccgttt tgaattatcg cacctatatg
     4801 ccgatcactt gccgaccaga gtttactgag ggcggtgtga cagcgaaaga tatctatcat
     4861 ccattgttaa aagaagcggt agttaatcca gtcaactggc agaagaatac attagtcacg
     4921 ggttccaatg catcaggaaa atctacgtat gtcaaaagta tagccatcag ctgtattcta
     4981 gcacagacga tccaaacagc gattgcagaa cagtttaccc tgcaacctgg acatgtgttg
     5041 acttccatgg caatcgaaga tgatttattt gaaggcgaca gttattttgt ttcagaaatc
     5101 aaatcgatca aacgtttgct ggaccaagtg gcttccaaag aacgctgcta ctgcttcgta
     5161 gatgagattt taaaaggaac aaatacgatc gaacggattg cttcttccgc ttcagtcgtt
     5221 cggtggcttg cggagtatcc atctttagca tttgtcgcca cacatgatat cgaattgaca
     5281 gagattttaa agaattattg tgagaatgtc cactttgaag aacaagtaac agaaggaaaa
     5341 ggaatcagtt ttgatttcaa attaaaagaa ggaccttcaa ggacccgaaa tgcgattgcc
     5401 ttgctgaaag ttttgaatta tcctaaatcc atagttgaac aggcccaaaa agaaagtttg
     5461 ttgttcgatg aacagcgtca gtggtatccg tttgattaac tttatcaaac gaagcatgat
     5521 gcgtgggtcc aaaaagacga ttatcaaaag aagtacattg atgccaaaaa agtggcagaa
     5581 ttcaatggga acgagtctgc tgtccagatg atcttggaag atttagcaaa agacgataca
     5641 aaataaacca cgctgtcttc cttctagcag cttttatcga aaaaacttag catcagcgac
     5701 gagacgcacg ttccatggca aagttctccg taaaacaaac agcgaaaaca gaaattgaaa
     5761 ataactattt ccattttatt tatttcggtt aaaataaaag aaaaaagctt tcgataaaag
     5821 gacggaggaa agcttaacat gaataaaaaa gacaagaacc tcagttataa ggatcttcta
     5881 tatgtcatcg tagtagttgc tgtcggtact tttatgattt taaaagttcc agagttgttt
     5941 agatagttat tctatcgaag atgatcatga actagaaaac acatctcctc tttgttagca
     6001 ggtgtgtttt tgtgtggaag ccaagttgtt ttttcatctg ctgtattcca tgatacaatt
     6061 aagaatcatg tctataagga gataggaaat aaatggcaaa tgcgcttgaa ataaataatt
     6121 tgaaaaagat ctatggaagt ggggtcgaag cattgcgggg aatcgatctt tcggtgaaag
     6181 aaggagattt ctatgcgcta ttagggccaa atggagctgg gaagtctaca acgatcggga
     6241 ttattacttc tttagtcaat aaaacatctg gaaaagtgaa tgtcttcggt tatgatctgg
     6301 acaaggatct agttcgagca aagcaacaga ttggattggt tccacaagag ttcaatttta
     6361 acccttttga aacggtccag caaatcgtcg tgaatcaagc aggctactac ggtgtctctc
     6421 gcaaagaagc gatgaaacgc agcgaaaaat atttgaagca gtccaatctt tgggaaaaga
     6481 gaaatgtccg tgcacgtatg ctttccgggg gaatgaagcg aagactgatg attgctaggg
     6541 cattgatgca tgaaccacgt ttgctgatct tggatgaacc aacagcagga gttgacatcg
     6601 agttgagacg cgagatgtgg gagtttctac gagaattgaa cgaaaatggt acaacgatca
     6661 ttttgacgac gcattatcta gaagaagctg aaatgctttg tcgcaacatc ggaatcatcc
     6721 aatcgggtga actgatcgag aatacaagta tgaaagagct tctgtcaaaa ctgcaatttg
     6781 aaacatttat ctttgattta gcgccatatg ataccaaacc tgtgattgaa ggatatgctt
     6841 gtacatttga agatgagcag acaatcgctg tcgaagttga acgaaatcaa ggggtgaacg
     6901 gaatcttcga tcagcttaca agacaaggaa tcaaagttct ttctatgagg aataagtcga
     6961 atcgtttaga agaactgttc ttgaaaatca cagaagaaaa taatcatacg gaggagaaaa
     7021 atgtttaatc tttattttac tgcattaaaa agtttagcta tcaaggaaac gaaccgctat
     7081 ttgcgtatat gggtccaaac actggttccg ccagtgatca cgacatcctt gtattttgtt
     7141 attttcggaa aaatgatcgg tgggcggatc ggtgacatgg gtggtttttc atatatggaa
     7201 ttcatcgttc ctggtttgat tatgatgtct gctatcacta gttcgtatgc caatgtttct
     7261 tcgtccttct tctctcaaaa atttcagaaa aatatcgaag aaatcttggt agcacctgtt
     7321 cctacgcatg tgattatttg gggctttgtg attggtggtg tgggtcgcag tattctcgtc
     7381 ggctctctcg taacgatcat ttccttgttc tttgtaccac ttcatgtgta ttcatggttc
     7441 atggtcatca ttacattatt gatgacggca atcttgtttt cattagcagg actgctcaac
     7501 gggatttttg ctcagtctta tgatgatgta tcgatcgtgc ctacatttgt cttgcagccg
     7561 ttaacttatc taggtggcgt tttctatgcc atctctatgt taccgccctt ttggcaagct
     7621 gtttcaaaag tcaatccgat cgtttatatg atctccggtt tccgttacgg attccttgga
     7681 acgatcgatg tgccagtagc aacatcgatg atcgtacttg tgttattcat cgttgttttg
     7741 tacgccgtta cttggtattt gatcaataaa ggaagaggat tgagaagtta
//
LOCUS       DACENJ010000122         7764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.125, whole genome shotgun sequence.
ACCESSION   DACENJ010000122 DACENJ010000000
VERSION     DACENJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.125"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1349
                     /locus_tag="IW748_001880"
     CDS             <1..1349
                     /locus_tag="IW748_001880"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1104789.1"
                     /translation="FMRGFIDWQIQQLNNQQLFKGNVAIIQPLEQGLGNMMNQQDNLY
                     TVILQGLSNGEIVDTSEIITVVEQMINPYDEWDAYLSLAENDDLAYIVSNTTEAGIQF
                     VETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGFTIIPCELIDRNGERLKEIILRYAEK
                     WDLEPAFVEWLEKENVFCCSLVDRIVPGYPREDAGVFNQRHNYEDQLMVKAEPFMLWV
                     IEGPKELENQLPFKQAGLNVIITDDMTPYRERKVHLLNGPHTAMVPLALLAELETVEE
                     VMKDPLFSAYVDQLFNLELIPMLSLPKDELAIYADQIKERFLNPFAHHKLEAISLNSV
                     SKFSTRLLPVFKKYIEEQNQVPPLITVSLAALLLMYRGDQVKPHDDEKTISEFTDAWS
                     DNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEIAAKIDQKGVRSVLQEMKGTMVHA"
     gene            1342..2841
                     /locus_tag="IW748_001881"
     CDS             1342..2841
                     /locus_tag="IW748_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1104790.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            2880..4274
                     /gene="uxaC"
                     /locus_tag="IW748_001882"
     CDS             2880..4274
                     /gene="uxaC"
                     /locus_tag="IW748_001882"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1104791.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            4433..5116
                     /locus_tag="IW748_001883"
     CDS             4433..5116
                     /locus_tag="IW748_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1104792.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            5118..5954
                     /locus_tag="IW748_001884"
     CDS             5118..5954
                     /locus_tag="IW748_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1104793.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            5965..7719
                     /locus_tag="IW748_001885"
     CDS             5965..7719
                     /locus_tag="IW748_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104794.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         2479 a         1499 c         1558 g         2228 t
ORIGIN      
        1 attttatgcg tggatttatt gattggcaga ttcagcagct gaataaccaa caattgttta
       61 aaggtaatgt tgcaatcatt caacctttag aacaaggtct tggaaatatg atgaaccagc
      121 aagataattt atatacggtc attttacaag gactcagcaa tggcgaaatc gttgatactt
      181 ccgagattat caccgtagta gaacaaatga tcaatcctta tgatgaatgg gatgcttatc
      241 tatctctagc agaaaatgac gacttggcct atatcgtctc caatacaaca gaggcaggaa
      301 tccaattcgt ggagactgat aatttaacag atactccccc acagagcctc ccgggcaaac
      361 tgacagcctt tttataccga cgtttcaaat taaacaaagc tggctttacc atcattcctt
      421 gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat cattttacgc tacgcagaaa
      481 aatgggattt agaacctgct tttgttgagt ggttagaaaa agaaaatgtt ttctgttgta
      541 gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga tgcaggtgtt ttcaatcaac
      601 ggcataacta tgaagatcaa ttgatggtca aagcagaacc attcatgctt tgggtgatcg
      661 aagggcctaa agaattggag aatcaactac cattcaaaca ggctggcttg aatgtcatca
      721 tcacagatga tatgactcct tatcgtgaac gtaaggtgca tttgcttaat ggaccgcata
      781 cagcgatggt ccctcttgct ttgttggcgg aattggaaac tgtagaagaa gtaatgaaag
      841 atcctttatt ctccgcatat gttgatcagt tattcaatct agaattgatt cctatgcttt
      901 ctttacctaa agatgaatta gccatttatg ctgaccaaat caaagaacgt ttcttgaatc
      961 cttttgccca tcacaaatta gaagcgattt cattaaatag tgtttcaaag ttcagcaccc
     1021 gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa tcaggtacca ccgttaatca
     1081 ctgtttcact agctgcgcta cttcttatgt atcgaggcga tcaagtaaag ccacatgatg
     1141 atgaaaagac gataagcgaa tttactgatg cttggtctga taatggaaca gctattcctc
     1201 gattattgca aaacgctgcg ctttggggag aagatttgtc tcaaataccg aatgtgacag
     1261 atacagttca agaaatagct gcaaagatcg atcaaaaagg tgtccggtca gtattacaag
     1321 aaatgaaagg aacgatggtc catgcttgaa accctgcaac caacaatgaa attaaatcga
     1381 cgagacagtg tggtcgtcgc attaacccct attccaaaac aaacacaact gacgattgat
     1441 cagcaaacga tcaccactct tgaagatatc ccgcaaggtc ataagattgc attgggtgat
     1501 ttgaaagctg gagacaacgt gatcaaatat ggctatccaa tcggtcatgt gacgacagac
     1561 gtaactgccg gtcaatggct gcacactcat aatgtaaaaa ccaatctttc tggtgaattg
     1621 gattatcgat atgaaaaaga tgtccatcca agtcattacc tttttgagaa ccgaacattt
     1681 caaggttact tgcgaaaaaa tggaaaagta gggattcgta atgatttgtt tttagtacct
     1741 accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca aacagttcaa ggaaaaacat
     1801 ccagatctgg gacagtttga ccatattacg attttaaagc atccttatgg ctgttcgcag
     1861 cttggaaaag atcatcaaaa tactcgagaa attttagcgg acgcagtaaa tcatcccaat
     1921 gccggaggag ttttagtatt tggattaggt tgtgagaaca atacagttcc tgaattcaaa
     1981 aaaattttag gtgaatatga tgaagaacga gtgaaatttc tagtggctca agatgtttac
     2041 gatgagatcg aacaaggtgt ggctctatta gaagagcttt taacagcggc ggaaaatgac
     2101 cagcgcatat cagtcccgct ctctaagttg aatgtcggtt tgaaatgcgg cggttctgat
     2161 ggtctatcgg gaataactgc taatccttta cttggtgcat tttctgacta cttgattgct
     2221 caaggaggca gcacgatctt aacagaagtg ccagaaatgt ttggcgcaga acaagtattg
     2281 atggcacgag cggaaaatga agaagttttt gactcaatcg ttgacttgat caatgacttc
     2341 aaacagtatt tcttgtctta cggggaacca gtttacgaaa acccatctcc tggtaataaa
     2401 gcaggtggga tcactacatt agaagataag tctttaggct gcacgcaaaa atcaggaagc
     2461 tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca gaaaaccagg gcttagttta
     2521 ttgcaagctc ccgggaatga tcttgtagct gcctctgctc ttgcttcatc cgattgccag
     2581 ttggtcttgt ttaccacagg aagagggact ccgtttggct catatgtccc aacggtcaag
     2641 gtatctacga atacaactct ttttgaacgc aaaggtcatt ggatggattt taatgcaggc
     2701 gtcttattag aacaaccaat ggaagtaatc ttagaatcgt ttgttcaaaa aatcatcgct
     2761 gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg ttcgagaaat tgccattttt
     2821 aaaaacggag taacactttg attttcataa aacagtataa aaagaaagaa ggaagattta
     2881 tgtttttatc tgatgatttt ttattaaaag acgattgggc gaaaaagctt taccattcct
     2941 acgcaaaaaa catgcccatt attgattatc actgtcactt agatccaaaa gaaatttacg
     3001 aaaacaacaa tttcagcaat ctgacagaag cttggttagg aggcgatcat tacaaatggc
     3061 gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac aggaaatgct tctgattttg
     3121 aaaaattttt agcttggtgt caaaccgtac caaaaattat cggaaatccg ctttacagtt
     3181 ggtcacatct tgaattgaag cgtttcttcg gtattgactt gttgataaat gaagaaaatg
     3241 cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc tcctgatttt cgccgtcggg
     3301 aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac agatgatccc gcagacgact
     3361 tgcactatca tcagttgctt gctcatgaag aaccagattt gaaggttcta ccttcattcc
     3421 gtccagataa agcactcaat attgaaaaag aaggatttaa tgcatgggtc acactactgg
     3481 aagaagcagc tgaccaaacg atcaccacct atcaagaact gatagaagtc ttagggcagc
     3541 ggatagaata cttccatcaa catggatgtc gtgtttccga ccacgcttta gacatcttac
     3601 gctataaaga agcggatgaa acagtattag aagagatttt tcaaaaagca cgagctaaca
     3661 aactattatc ttcagatgaa atcgatgctt atcgtacaga aacactgata catttgaccc
     3721 acttctatca ttctcataat tggacgatgc agctgcacat ccatgcttat cgtaactgta
     3781 atacacaaat gttggaaaaa ttagggcccg ataccggcta tgatggaatg aatgatcttt
     3841 ctttgactat tcctctgcaa aaattattga accgtgcaga agaaacggat cagttgccaa
     3901 agacgatcct ttattcattg aatccgaatg actatcctgc gattttagct ttgatgggct
     3961 gtttccaaaa agaacagaat ggaaaactgc agttaggttc tggatggtgg tacaacgata
     4021 ctcgagctgg tatgcgcgat caattgacac aatttgctga tggtagtgct ttggggaatt
     4081 ttgtcggcat gctgacagat tcaagaagtt tcctgtccta cactagacat gaatatttcc
     4141 gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg aggagaagca cctgaagatg
     4201 aacaactttt aggaagtcta gttcaagcga ttagttatac aaatgctaaa aattactttg
     4261 gcttttttgc ctaattgatt aatagtaaca gtaacgaatc aaaaacagga tagtgaaata
     4321 acggaatccg tttatccacg ttttagagaa gaagacggac caaatttgct ccgtcttctt
     4381 ctcttgctgt tctgcttcat tgcctataaa cctttatgaa agaagagata acatgcctac
     4441 tatccatatt gccggtgatt ctactgcggc agaaaaagat gaaaacaagc gtcccgagac
     4501 aggttgggga gaaaaaatct ctgcttattt ttcacccgag atccagatca ataaccaagc
     4561 aaagaatgga cgcagcacca aatcatttat ccaggaagga aggttagccc gtctagcttc
     4621 acagtttcag ccgaatgatt tcttatttgt acaatttgga cataacgacc agaaaatcca
     4681 agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa aatctgcaga tattttttga
     4741 atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca tctgtgacgc gacgagatta
     4801 cttagaaaac ggaacattga atccagatat acttggagac tatccaaaag cgatgcgtgc
     4861 atttgctgag aaacaccata ttcctctttt agatgtgttc tcaagatcac aagaattgtt
     4921 ccagaccttc tcaaaagaag agacaaaaaa attctacctg catcttatac ctgatacttg
     4981 taaaaattac ccagaaggac taaaagacaa tacccacttt agtccagaag gcgctgataa
     5041 agttgcccga ataattgttg aattgataaa agaatcccgt ctgcctcttg ctaattattt
     5101 acaaaaagga gtttgagatg tttccattaa atttaggcat ccgtgcccac gatttagatg
     5161 ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct tcacttgtcc catatccaat
     5221 ttgcaccaca aaaagcattt cctgaacaac ttaaattaac tgacatgaca cttgggagtg
     5281 ctgcctattt tggtgaatac ttccaaaaaa acaatatcga actttccatt ttaggatgct
     5341 atatcaacct atccagcaaa aatcaagaag ttcgtgcaca agcattggca actttcaaac
     5401 agcaaattgc tctgtgctct gcctatcaag ccaagatgat tgctacggaa accggtagtg
     5461 tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc ttatcagatc gccagaaatt
     5521 ctatcattga attagtcgct catgcagaaa attttggcgt aactgttgct gttgaagcag
     5581 ggatcaatca tccaatctat aactatcagt tagctaaacg tttaatcgat gaagtagctt
     5641 caccaaattt aaaaatcatt ttagattgtg cgaatttgat gcataaagaa aatcatgctg
     5701 atcaggaaaa agtgatccga ggtgccttgg acaatttaca tggttatatt acagcgcttc
     5761 atctcaaaga ctatatcatg gaaaatggaa agatccgcat ggtacctgtt ggcaagggct
     5821 gtttagattt ccgcccggta ctgcattata tcaaatatga gcgtcctcta atgtacgcca
     5881 ctcttgaagc tactcctgag atttatgttc ctgaagcaat agaacacttg cagtttattt
     5941 accaagattg ttaaggagaa gattatggag gaacgaatac agaaaagtta tcaaaaatat
     6001 catgcaaatg cttttcagac attgctgcat ctttatcagg gaaattattg gcgttttgcc
     6061 atgtcaggac tcttttttat tatcaaacat cttccatctt ggttaatgcc gatcgctatt
     6121 gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc agttgcaggc catttatttc
     6181 aacgcttttt tgatggcttg tctgattggt caaaatatat tgacgaatta ttttcatgta
     6241 aaataccata gcacttcgat ccgtcaagta gaagctggac taagagcgac ccttatcaag
     6301 aaaatccaag aactatctat tccttatcaa aaagaattgc aatctggacg aatccagtca
     6361 aagatcatcc gtgatgtaga agctatccaa acgttgtcct ctcaagtatt tgtcagcgga
     6421 ttaaatatcg ctgtcaattt attcgtagct ttaggtatca cgctttataa aagtcctgtt
     6481 gttttcatgt ttttcttatt gactgttcct ctcgccgcat tcgttactct ctttttccgt
     6541 aaaaggatca gacagtccaa ctcagacttc aggcaagaaa tggaagagac ttctgcaaaa
     6601 gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc atgggttaga aaaacacgaa
     6661 acaactcgct taaataagca ttttactcaa atctatgaaa aaggattcca cttagatatc
     6721 ctgcaatctg ttttcggatc agtcagctgg gctagttttc agatatttca gctgatttgt
     6781 ttgattttca ctgggatatt agctttacaa gggcgcatcc aacccggcga tgtcgtgatg
     6841 tatcagacgt atttcacgac gatcgtcaat tccgtttctg gaatgatcac gctgattcct
     6901 acgatttcaa aaggcatgga atctatcact tctattggcg aactgttgat tgctggcgat
     6961 gtagaaactt atcacggtaa gaaaaaaatc caacatttgg atggaaattt tcgttttgag
     7021 gatgtttctt ttctttatcc ggactcactc aaaccgatta tcgatcattt ttctctagag
     7081 gtgaaagctg gagaaacaat tgcttttgtt gggccatctg gctctggaaa atctacactc
     7141 cttaacttat tgattggatt tatccagccg acttcaggaa aaatttttct agatgatcag
     7201 cctttggatg aactggatat gcgaagtgtg cgaaattatt tagccgttgt tcctcaaacc
     7261 actttgttgt tctccgcttc gatcaaagag aacatcactt atggcttgaa aaacgtatca
     7321 aaagaacgtt tagatgaagt catcgaagct gcgcaacttt cttcactgat tgaccagctt
     7381 cctgatggtc tggatacctt agtaggcgaa catggcaaca agctgtctgg tggccaaaag
     7441 caacgtatct ccattgcacg agcgttgata cgtcagccaa aaatcatttt gctagatgaa
     7501 gcaacctcag ctttagataa ccagtcagag aaaaaaatcc agcaagccct gaattacctg
     7561 acagaaacac ccactacttt tattgtggct catcggcttt caacgatcaa agaggcagat
     7621 aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag gtacttacga aaaactgtta
     7681 gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac aaaaaatagg agctatgaca
     7741 agacttttgt cgtagctcct attt
//
LOCUS       DACENJ010000123         7664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.126, whole genome shotgun sequence.
ACCESSION   DACENJ010000123 DACENJ010000000
VERSION     DACENJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.126"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            53..1792
                     /gene="yfcC"
                     /locus_tag="IW748_001886"
     CDS             53..1792
                     /gene="yfcC"
                     /locus_tag="IW748_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1104795.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1949..3133
                     /gene="iadA"
                     /locus_tag="IW748_001887"
     CDS             1949..3133
                     /gene="iadA"
                     /locus_tag="IW748_001887"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1104796.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3244..3447
                     /locus_tag="IW748_001888"
     CDS             3244..3447
                     /locus_tag="IW748_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1104797.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3548..4210)
                     /locus_tag="IW748_001889"
     CDS             complement(3548..4210)
                     /locus_tag="IW748_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104798.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4223..5725)
                     /locus_tag="IW748_001890"
     CDS             complement(4223..5725)
                     /locus_tag="IW748_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1104799.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNLKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6273..>7664
                     /locus_tag="IW748_001891"
     CDS             6273..>7664
                     /locus_tag="IW748_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1104800.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIF"
BASE COUNT         2348 a         1367 c         1667 g         2282 t
ORIGIN      
        1 tatccatttt ttattcatca aaattactga aaaaagaaag gaagaaataa atttgaagaa
       61 aacaaattct tcaggaaaac cgaaacgcga cttcactaaa ttatcgacac cccatacgta
      121 tgtcattatt tttggtgtag tcatctttgc atggatactg acttttgtcg ttccagcagg
      181 aaagttcagc acgcaagata ttgaatacaa agatgcaaat ggcgaaacaa gtactcgtac
      241 tgttttacgt caagattcct tccgttatgc ttacgagctg gataagtcgt atgtgtttga
      301 tcaattagaa gaactgcaag atcatccagc agaacgtgaa aaactggatg ttccggaaaa
      361 aggattggaa aaagtcattg ctgacggaga aaaaaatctt acgcaggaaa aactagatga
      421 aatctccttg acagatgatg tgctttacga tcagtacggc gaaaatatct atgatacttc
      481 taaaaaattg cataaaacag ccaaaatatg gggaacagat gattttggcg gctttggttt
      541 cttaaatttt gtttttgaag gactagtatc tggagataag tatggatctg ctgtagggat
      601 cgctgcattg atccttgttg ttggaggtgc ttttggtatt attatgcgta ccggagcgat
      661 cgacgcaggt atctatgcgt ttatcagtaa aactaaggga ctggagcgtt tagctttacc
      721 gctattattc tttgcttttt cattcggtgg cgctacattc gggatggcag aagaggtaat
      781 tccattctca atggtgatgg ttccatttgt cattgcgtta ggatatgact cgatcgtagc
      841 agtaaccgtg acttatgtcg catctcaagt cggaaatgcg acctcttgga tgagcccgtt
      901 tagcgtagcg gtggctcaag ggattgctgg aatccccgtg ttgtctggtg cgacattccg
      961 attgattatg tgggtagttg ttactgcatt agccgctggt tatatgatga tatatgcaga
     1021 aaaaatccgt aaaaaaccag aaagttcgtt gacttacaaa tcagatgatt atttccgttc
     1081 tcatatcaaa aagacatctg atgaaaataa aaaattcatg cttgggcata aattgatctt
     1141 attagagatg ttagttgtcc ttgtctggat cgtttggggc gtgactcaaa aaggctatta
     1201 cattccagaa atcgcttctc aattctttgt gatgggatta gttgctggaa tcattgctgt
     1261 tcttttcaaa ttagatggaa tgaaagtcaa tgacatcgct tcttctttcc aatctggtgc
     1321 agctgattta gctggtacag caatcgtagt aggtatggcc aaagggattc ttcttgtctt
     1381 gggaggatct gatgcatctg ttccttcagc tttgaatacg attttgcatg ggatcgggac
     1441 ggcgttgacc ggtgttccag ctgtcattgg tgcttgggcg atgtacatct tccaaagttt
     1501 gttcaacttg gttgttacat caaattcagg acaagcagct ttgactatgc caatcatggc
     1561 tccactggca gatttagtag gtgtatccag acaagtagca gttcttgctt atcagcttgg
     1621 ttcaggtttt atggacgctt ttacacccgt atcagccagc ttgattggtg tgctaggtgt
     1681 tgcacggatc gaatgggcaa aatgggcaag attccaaatc aagatgcaag gattcctgtt
     1741 tgttttagga actattttca tcatgattgc cattgcaata ggtctgcagt agtactggag
     1801 acctgtaaat aaaaaagtaa tccaaacagt agataaggac tccaagaacc gtcattttac
     1861 ggtaaaatca aaggagtaaa gatcctatcg tttggattat tttttaagat cctatcatca
     1921 gttatactta aagaaagagg aaaaatgtat gaaaatcatt cgacaaattg aagtgtttgc
     1981 accagattac ttaggaaaaa tggatgtact gattgccggt gggaagattt tggctgtaga
     2041 agaacagcta gaaggcggct acgaaggagt ggaagtagaa gaactttcag gtgagggaaa
     2101 agtactaacc ccgggtttca ttgactgtca tttccatatt ttaggtggag gaggagaagg
     2161 cggttatcaa aatcgaacgc cagaagtcac attgagccaa ttgactacag ctggtgtaac
     2221 gacagttgtc ggttgtttgg gaacagacgg agaaggtaga gatatgactg cgttgatcag
     2281 caaagcgaaa ggtctggaag cagaaggaat ctccacttat gtatacgaag gttcgtaccg
     2341 attgccggta aaaacagtaa cagattctat catcaaagac tttttgacaa ttgataaaat
     2401 tatcggaatc ggagaaatcg cagtatccga tcaccgttct tctcagccaa gttttgaaga
     2461 gttcgctcgt gcagtagcag atgctcgtgt aggaggcatg ttatccggaa aagctgggat
     2521 catcaatgtc cacttaggcg gaggaaaaag aaagctagag ctattgacac gaatcgtaga
     2581 agaaacagaa attccgatca cacaattttt gcctactcat gcaaatcgca caccagaatt
     2641 atttgaagct tgcgtggcat ttgccaaacg tggtggcacg attgacttca ctgctagcga
     2701 agatcctgat ttttgggaga agacagatgg tgaagttcgc ttcagcaaag cactgaaacg
     2761 attgatcgag gaagatgttt ctttagataa tttcacgatg acctcagatg gacaaggaag
     2821 cttgccttat tttgatgaaa acaatcattt cctagggtta ggagtaggaa gtgccaaggc
     2881 attgctagta gggatcaaag aagctgtgca aaaagaaagt atccctttgg aaatcgcctt
     2941 acgagctata accagtaatc cagcacgtat attgaaattg gataaaaaag gaaaaatcga
     3001 aattggtgct gatgcagatc tgtgtatctt ggataaagaa acgcttgata ttgatacagt
     3061 tatcgctaaa ggagaaataa tggtccaaga aaaagaagtc aaagtatggg ggactttcga
     3121 aaaaagtttt taatcaacag ctctaactat cttttttaga aaacgagcgt tctgtttgtc
     3181 tgtatctgtt gttgatgtta ccctgaaggt agatcaataa acaaactaag aggaggaagc
     3241 acaatggcag atttaaaagg acgttctaaa gatatgaaag ataaagtagt cggtgaagga
     3301 aaagaagcat acggcaaagc aacagacgac aagagcaaag aaacagaagg gaaagcacaa
     3361 tcactaggtg ctgacatcaa agaaaaagct cgaaatttag gcgacgaagt caaagacaag
     3421 ttttctcgaa aagacgaaaa aaaataaaaa ccacttatta ctaattgaat caagcaaata
     3481 aaaaatccga accatatctg cgagacagat atggttcgga ttttttattt gatcattatc
     3541 ctgtttttta aacttcgatt ttttgaaatt ttttattttt tagttcaaag acttcatcac
     3601 agctttttgc tacttcaatc tcatgagtca cgatgataat acagcgattt tgttcatggg
     3661 cgatttcttg gaaaagtttg acgatatctt gtgaagttgt ttcatctaga tttccagtag
     3721 gctcatccgc tacgattagt tgatgttcac agcagatagc acgaacgata gcgactcgct
     3781 gctgctgacc tcccgaaagt ttggtcacct gtttttttgc taatatttca tcaattccta
     3841 cacgagccag tcgatctaaa gcaacttgtc gataattctc ttgcttggtc ttagtgattt
     3901 ccattgctgc tatgacgttg tctagagccg ataaataagg aagcaagttg taagcttgga
     3961 aaataatcga gacatcattt ttacgataac ttcttaaccc gattttgttc agtgaacgat
     4021 cttgatagta tacctttcct tcttttggtg catctagtcc agaaataagc gaaagaaaag
     4081 tcgtttttcc agaaccactt gctcctaaaa tagcgtacat ttttccagct tcgaaattca
     4141 gttctacatc tttataaagc gcatcttgcg gatcttgata ccaaaatccc aattgttctg
     4201 tccgtaacat caaattcctc ctttagttta ataggatttt tcttgggttt aatcttaata
     4261 tccctataga agctaaaaag acagaaaaca gactgattcc aaatgctact ccggctaata
     4321 gtcccaattg tgcaggtttg acattgattt ccaattcgct gacttgatca gaaacagcaa
     4381 atggattatt cgtggttcct tgttgaccat cgcgttgacc gccttgttct tgtctgttac
     4441 ctggcatttg tccaccgcca ggagcttgct ggttttgtgt cgtggtttct gtctgttggt
     4501 caagtagctg ctgtcccacg gcattcgcga cgacgtttcc gctaagcgaa gcaactccta
     4561 atgcaagaat catacaaata gccacttcgg taaacaattg gagaatgatt ttacttctgg
     4621 attctcctaa agatagcaag acgcctaact catgccgacg ctcccgaatg gtaatcataa
     4681 tgatcagagt aagtatgata ataccggcta cagccactag taaaacaaca tttttagaaa
     4741 aacttgccac gttgtttaaa ggttccaaca ttgactgata catggaatca tttgattgta
     4801 agctaaatgt atctgtgtcg atcagcttct cagcttcttt gagaaactca tccattttat
     4861 ctggatcaga tagagtgtaa atagcagaat ctatcgtgtt atcgtcagat gtcccattta
     4921 gttcatttgc aaaagtgtaa gaagtgtaaa gtgtattcga cggattcatg aagttgaatt
     4981 tcatacccat actgccgctt gtttcgctgg attcataaat tcctttgatc gtcatttcat
     5041 aagtggtatc ttcatcttca gtactcgtga ttgtgaaagt atctccgact gccaggtcat
     5101 ttgtttctgc tagagtagaa tcaatgacta cattgtttgt gcctttgtcg ctgtcatcta
     5161 ttccttcacc atccgtgatt tttgctgttc catcagaaaa attactattt tgtgcagttg
     5221 ctgacacacc actgatttga aagtctgctt gagtcatttg cggattcccg cccattcctc
     5281 cagcaggttc accgcctgca tcagtagttt ctgaactgtc ttcacttgaa tcagaactag
     5341 tgatggcagt tattccatct tttgccatag ctgaggcgga actttcaaag gaataactct
     5401 ttaaattatc taattcagct attttttccg cgtccgttaa ggaaactggt gtaagagaaa
     5461 aactgcctgg atctggacgt tcttcagtgg tagaaccgct tgaagaagtt tgttcctgtt
     5521 ttttaaatga agcttctcga ttggcactta aagtcactgt tgctccaact tctttttgtg
     5581 cttgattcgc tgcttcttct gcagcgcttc gaatcgtgag gccagctagc acaaaaataa
     5641 gaatggctgt gaacacggca aacaataaca atgaacgtcc ccattttact tttgtacttt
     5701 tccatgcacg tttcataaaa ttcatatttt gcatccctcc tagtagatgc atttgataat
     5761 agacaaccta tcttaaagct agcttaaatc ttatgctgtt tgcacaccat caacatgagg
     5821 ttctgtatat ataaaaagtc actatagtta ttgcaaaaaa tgtggtaaaa tgaaaaaagt
     5881 atgtttcaaa ccgacgaaga agaaatgaaa gaggaatagc aaaagaaatc ctagatgcta
     5941 cagagggatt tttacgtgca tagttgttct aacaattgct gatactagcg atttagttta
     6001 tttgttgtat aataatgggg aacagctgaa tacttatata agtattctgg ctatagcgtt
     6061 tcgcaaaaga tccattttgt taatgaagta tatccaaaat aaaatgccca tccaactgtc
     6121 agaacgcgga tgtgctggta attttcctgc tgattctcca taagttgaga aatagtgatt
     6181 ttgcatatgc ttcattggaa tggctcatat atttagataa agaagaaaat cgacatcttt
     6241 aaacttggat gtcataagaa gaggtgaagt ttgtgagtac cattaaaatt atcccattag
     6301 gcggcgttcg tgaaaatgga aaaaacatgt atgtcgcaga agtgggcgag gatattttta
     6361 ttttagattg tgggctaaaa tatcctgaaa atgagttgtt ggggatcgac gtcgtaatcc
     6421 ctgattttac gtatttagaa gaaaacattg atcgtgtagc tggtgtcttt ttaacacatg
     6481 gacatgcaga tgcgatcggg gcacttcctt acttgttgtc aaaaatttct gtaccagtat
     6541 tcgggacaga gttgacgatc gagttagcaa aattgaatgt cagtcgtaat gaagcagcca
     6601 aaggatttaa ggatttccat gtggtagatg agcatacaga aatagacttt ggtgaaacag
     6661 tagtgagctt tttccgtacc actcatacga taccagattc agtggggatc aatctgaaaa
     6721 caaaagaagg tagtatcgtt tatacgggag attttaaatt tgaccaaacg gctatcccaa
     6781 tgtatcaaac agattatgca cgattggcgg aaatcggcaa agaaggagtc ttagccttac
     6841 taagtgaatc ggcaaatgca gaaaatcctc agcctgtagc ttctgaagcc caaatagcag
     6901 atgaagtatt tgatacgatc aaatattggg aagggcgtat catcgtcgct tgtgtagcaa
     6961 gtaatttgca acgagtgcaa caagtattgg atgcagcaga aaaagcacaa cgaaaagttg
     7021 tcttgactgg tcaagatttc gaacgaatta ttcgcacagc gatgcgttta gaaaaacttc
     7081 aattaccaag tgaagatctg cttgttacgt tgaaagacat gaaaaactat gatccagaag
     7141 aactgatcat tttagaaact ggccgaatgg gtgaacccat caagtctctg caaaaaatgg
     7201 caaacggtac acaccgtaat gtcaagattg aagaaggaga tttggtttat atcactacta
     7261 cgccaagcat cgcgatggaa accatcgtgg cgaaaacgga agatatcatt tatcgagctg
     7321 gtggaacagt gaagttgatc tctgagaata tgcgcgtttc tggccatgcc aatcctaatg
     7381 acttgcaatt aatgatcaat ttgatgaaac caacatattt tgtgccagtt caaggagagt
     7441 atcgtgaatt agctgctcat gcagatttgg ctcatgcagt agggatgcca tacaaaaata
     7501 tctatattac tggacgcgga gacgtgctgg aatataagaa caagcgtatg agtgttgcag
     7561 gaactacaag tgcagaaaat gtaatgattg atggattagg cataggtgat atcggaaaca
     7621 ttgttttacg tgatcgaaaa atcttgtcag aagacgggat tttc
//
LOCUS       DACENJ010000124         7581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.128, whole genome shotgun sequence.
ACCESSION   DACENJ010000124 DACENJ010000000
VERSION     DACENJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.128"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..358)
                     /locus_tag="IW748_001892"
     CDS             complement(<1..358)
                     /locus_tag="IW748_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HAQ1104801.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQ"
     gene            complement(360..1124)
                     /locus_tag="IW748_001893"
     CDS             complement(360..1124)
                     /locus_tag="IW748_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1104802.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1132..1809)
                     /locus_tag="IW748_001894"
     CDS             complement(1132..1809)
                     /locus_tag="IW748_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1104803.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(1846..3231)
                     /locus_tag="IW748_001895"
     CDS             complement(1846..3231)
                     /locus_tag="IW748_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1104804.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3441..3525)
                     /gene="ffs"
                     /locus_tag="IW748_001896"
     ncRNA           complement(3441..3525)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW748_001896"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3548..4063)
                     /gene="tadA"
                     /locus_tag="IW748_001897"
     CDS             complement(3548..4063)
                     /gene="tadA"
                     /locus_tag="IW748_001897"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1104805.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4170..5027
                     /locus_tag="IW748_001898"
     CDS             4170..5027
                     /locus_tag="IW748_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1104806.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5108..5443)
                     /locus_tag="IW748_001899"
     CDS             complement(5108..5443)
                     /locus_tag="IW748_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104807.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5510..6880)
                     /locus_tag="IW748_001900"
     CDS             complement(5510..6880)
                     /locus_tag="IW748_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1104808.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(6958..7494)
                     /locus_tag="IW748_001901"
     CDS             complement(6958..7494)
                     /locus_tag="IW748_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1104809.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2130 a         1622 c         1222 g         2607 t
ORIGIN      
        1 tttgcaaata tttgataaac aaaatactcg ggatcaaagt gatgaaaaac acagcataat
       61 ccactaaaaa caaaattttc acttcataaa agtgatgggc tccgctagca gaaactggga
      121 aatctggcaa gctgaggatg gttttgaagg gattattgag ataattcatt aggtgatcaa
      181 aatttttcag taacgtctct tgatctaaag aagtataatc caaaatttgc aaatgtccga
      241 tatcaaatac gtatagtggt cgaaagttga tcgtcacacc aactgccaga ctgatcagtg
      301 tcagaaatag gctgaacaat ccagcatatt ccatccactg ccatctcgaa tttttcatat
      361 tagaagctcc attcatccag tgattgtttc acataggtag ctgggatagg caacccaggt
      421 acatcttctt cttttgtaaa acctgataaa acgagcaaag tatctatatt attgcgtatc
      481 ccagcttgga tatctgtttc atagttatct ccaaccatga ttacttcttc tttttgcagc
      541 cctaatactt caacggcttt atccataatg atggcttctg gttttccgat gtagatcggc
      601 ggcgtttggg ttgctgtctc tacaaaagag atgactgacc ctgctccagg aagaaggccg
      661 cgttcagtag gtatattctt atctgggttc gttccaatga aagtagcacc tttttgaata
      721 gcaagtgttg ctttaactac tttttcatag gtcagataat tatctaagcc taccactaca
      781 tagtcaggag cctcttcttc ccatgtaaat ccggcagcta aaatcaagtc aatcaatcct
      841 gcttctccga taacatagac ttttttccct tttccatctg ctttcataaa atcgattgtt
      901 gccaaagtag ctgtgtagac tgtctctgga gctacatgaa tgtcaaactc atctgctaga
      961 cgattagcta ctgtttctgg tgacttagtc gtgttattgg taacaaacaa gaaaggtaat
     1021 tttctttttt gcaattcttc cacaaatcgt ttaccggccg gtatgggttg tgtcccacga
     1081 taaatcgttc cgtctaaatc aattaaatat cctttatagt ccattcatcc actactcttc
     1141 cctttttctg atcgtaaatg ctgtttttgt tttttctact tgtttaggtt gttcttgttt
     1201 ttttactttt tcagaggttt ttgctgactc tgtttttcga tttttaataa ccggtttttt
     1261 gcgattagat gcgactggtc cttttttctc atcgatatac gcacgattat ggtttttttt
     1321 cttttttcgg cgctgagttg tcttttcttt tttaccgccg attctctcga tgacaaaata
     1381 cgcacaacca aaattgcaat attcgtaaag atagtcttcc aaggaatcga ttcgctgatc
     1441 tggatgcgcc cgtcgattat ccgcttggaa aaatcctttt aggcgtagtt gttcataacc
     1501 ccaatctcct acgatataat catatcgggc tagtacatca ctgtaacgtt ctccaagctt
     1561 ttctgcatta aatccttcac gataatcgga cacaagccga taatgtcttt cacctatcaa
     1621 aaaattactg tcatctaaca gtgtcactag ctcgcctttg actttttctg ctttaggctc
     1681 ctcaacgatt tcttccaaag cagctgtcag ttcttcggtg actgttgttt cctgttcagc
     1741 tgatgatttt ttcttagcaa tttctgtttt atgctcaacg ttctgctgtt cattttgttt
     1801 tgcagacatg cgttcaccac ctatcttttt tattatacct tgtttttatt tgattgggta
     1861 gtgagctttc aagtaatctc ctaccacact tcggataaat tcagggaaca aaaattcatt
     1921 ttttccagca atctcaatcg ttggaaaaaa tggaacaaac atcaaatgat ctacggtagt
     1981 aaaagtataa tttttattca gatcgattgg cttgttcttc caaaagactt gatggttaat
     2041 agaatcatat gtgattccat tatagaccat ctgcccaaaa atcttaccgc gaaatcccat
     2101 tcccttcata ggatagttac gtaaaaagtt acgatttttt tctatttcta aaactaagcg
     2161 gatcagatca tttccaccca atgtgacatt taacaagtgc atcggatgcg gcaacgcagt
     2221 gtgaagctga tcctgattga cccgcccttt tggaatttca gttaaaaaca gaccactgtt
     2281 taatattgct gcttccgtct ttcctcgttc tttcaccgcc tcaaaggcta cttggataaa
     2341 cgaatgctca ttgaagatat ctagactcag agaaaatggc aaatctgcta cttctttttc
     2401 ttttaataat tcatgcccac gttctagata actatttatt tcttgttcat cttctgtaaa
     2461 agctgtcatt gtttcggtag ggatggcttt ggctgaatgc ttaaggattt ttttctcttc
     2521 atcgatggtt aaatggactt caccgacata ctgcccgtac tttccagcgg ctgctatttg
     2581 gacatcattg attacgtgac cttctctgaa taaatgatgg gtgtgcgatc ctaaaataac
     2641 atcaattgct ggtatttctt ttgcaatttg gaaatcatcc tgaatcccga gatgactcat
     2701 caaaactaaa acatccactt gtgaacgtaa atcttcgact aattctggca aaatatcata
     2761 aggattccgt atgtcccagc cattcggatt ataagtaagc ggaaatggag cagtaaatgc
     2821 aattagtccg attttcgtct ggtgtttcgt tgtaatgatt ttatatggtt tcgtccatgc
     2881 cggtggctgg agcgtatttt tatcgaataa gttatccagg atgatatcaa actcagcatg
     2941 atcgtataaa tgattcaact cttctttgga atttcctacg ccttcattgt ttcctatcgt
     3001 cgctgcatca taaccaactt cattcatcag caagatattt tcctgtccat tcgttgcttc
     3061 tgacaatgga tgccaacgat ccacaaaatc acctagatct actgcaatcg ttgtcgtttt
     3121 ttctttcttt tcgttttctc gtttacgttg atccaaaaac cttctgatcc ttggccaatt
     3181 ttctaaatga gaatgcagat cattcgtatg taaaatcact attttttcca tgtaccctct
     3241 cgcctctctt tatctttatc ataccatagg cagctttttc ttccattgaa acaagctcta
     3301 tttcagagtt tttcctgcct tgaagcaaat tgtataagta caatattcga taaatcagtc
     3361 tggctaaaaa atcaggaaca ttcattttta cagtactata aaaataaaaa aaacagaccc
     3421 gaaagtctgt tgtcgtttca ttataatcag acacatggcg gaacctgctt agtgctgctt
     3481 ccttccagac ctgacacgct tcacaagccc accattgtcc tagcctttcg gcaaagaata
     3541 gtatacctta tttttcattc gttgaccagt ctttttttaa tttctttttt cgctggcgga
     3601 gctttcggaa aaagtcagtc aaaatcatcc cacattcgtc ttctaaaatg ccaccttcta
     3661 catatgcttg gtggttaaac cgctcgtcct ccaaaagatt catcaaggta ccggctgttc
     3721 cgccttttgg atcatacgcg ccgaaataaa cttctgctac acgagacaaa atcatcgcgc
     3781 cgctgcacat aggacaaggc tctaaggtaa caaaaagtgc agcatcttcc aagcgccagc
     3841 ttcccacttt ttcacaggct tccttgattg caagcatttc tgcatgtgta gtagcatctt
     3901 gcgtggtttc tctaaggtta tatcctcttc caatcacttt tccatccaat acaaccactg
     3961 ctccaatcgg aacttcatga agcatttctg ctttctttgc ttctgccaat gcaaggcgca
     4021 tccatttttc tttttctact gcatcaagat ttgtatttac caattaccct tccctgcctt
     4081 tgttttttct tgttttactt tagcatatga taaaaaggct gtaactaact tttttttcta
     4141 tggtaaaata aatacaagag gtgaacaaga tgaaaatgga taaattactt gctctttacc
     4201 cacaagcaca gaaaaaaaac tctccagcat cagaagcgga tatcctttct tttgcaattg
     4261 atgatgcttt tatctggatc aaacaagatt cattatctca acaagaaacc agccttttga
     4321 aagcgttatt ccctgtaatc aacgatcaaa aaaaacaccc atggtatcac tatcttttta
     4381 aagatgcttc ttgtgttcaa gaaaaaagct ttcgtttgat ccagcttcac gtcgaatcaa
     4441 aaggagaatt cttaaaaaaa gaatggcaag atactatttt ggaattattt cgctattctg
     4501 ctgatttctt tttttataca gacacagatg cgctcttagt cgaacaaata aaccgtgatt
     4561 atcttgatgc tactgaaatc aacggtattt ttctatcttt agatgctgat tttgatacga
     4621 caacgtctgt attcgttggt tctttccatt cacctggcac cgacctcgtc cctttgtttg
     4681 ctgaagaaag aaaaattttt ttaacagagc aaaacaatct ttatactcac agccgtactt
     4741 tttctatgca agatgtggct ttacattact atacaaaaga tactgtaaaa gatagtaagt
     4801 tgatcaaaag ttacatagaa ctgatcaaac aaaatgaaat ggaagtgatc attcaagaat
     4861 tatggaatga attaggcaat gtcagctctg ctgcaaaaac actgtttctt catcgtaata
     4921 ctctaaaata tcgaatcgaa aaatttcaag aacagtctgg tttcaatcta aaaaaagcca
     4981 acgatttact gttttgctat cttttattgc ttcatgaaca taattaaaga tagattaaag
     5041 gggttgtgac aataattttg tcacaacccc ttatttcgaa tgaatagtgt tcaaaagcta
     5101 gtttctctta catcattttt tcaacaaaat gcattagccg gtcttttaca tcttcggttg
     5161 aatctttgat tgaatcacca taaacagaga ttttcttttt tcctgatttg atttttgcta
     5221 acattcccat caaagaatcc aagtcactat ctgaaagacc atctacgatt tctagtaatt
     5281 tttcatttcc atcaaatttg tcatagacga atttttttac tttcgtccga gtagtcatat
     5341 gatggatctt ttccattact tttcccgata caacgactgc tgtcgctaca ctggcagcgg
     5401 ctgctacgct taatccgatt gttacttttg ttgatgtttt cataagtatc agctcctttt
     5461 tatctatctc catcatagca tagacaaaaa aataaacgaa ctaatccact cagtgaaatc
     5521 tcaatccttt agggaaaaag ttctttactg ttccttttac ttgtttccca aatccgacag
     5581 gtatcttatc taatactagc aaaacccatc cttgattccc atcacgttga aatgtttctc
     5641 ctgctgtgta agattgccat tccttttgag tgatcggcaa gcaaggtata ttctctatct
     5701 tttttgtagc aagcgctaat gcatagctag gttcaaatcg attctttttg aaatctccta
     5761 aatgcaaacc tgtacgtacg acttttaaac catcgagaga aggggctaac tcaggaactt
     5821 cccaaagatg gtcgttgaac actaaaagtc ttccagtagc ttcataatga aaatcatttg
     5881 aaaactccgt ccagagtttt tcctgctctt ttgtcatttg cactttactt ttttttcgtg
     5941 tcttcttttc tttatgcatc tgattttgtc catgaaaagt cagctttgca acaaaatgtc
     6001 cttcaccctg atctttatga ggccatatac gtattgtttt ttccaaacca gctacgcttc
     6061 cccattcaga acgtcctgaa ctaacggatt gagtcaaagg aatctcctca atcgttactg
     6121 gataattttc tacaagccag ctaatgatct cttcattttc ttctggtgca aacgtacaag
     6181 tcgaataaat cagttgtcct ttatttttta gcatttttat tgctgaagac aaaatctctt
     6241 gctgacgttt ttgacagtat agcggagatt cttctgtcca ctcttttatt gcatttggat
     6301 cttttcggaa cattccttct cctgaacaag gagcatcaac cacgatccga tcaaaaaagc
     6361 ccgaaaaatg agggacaagt tctgcgggtg catgatttgt cactattgcg ttcgaaactc
     6421 cccatcgttc tatattttct gataaaatct tcgcacgttt cggaaagatc tcattcgtca
     6481 ctaataaccc tttccccttc atttgagcag ctaattgggt tgatttacca ccgggagccg
     6541 cacacaaatc caaaactttt tcaccgggtt ttgctgcagc agctgttcca acgatcattg
     6601 cacttggttc ttggctatat tcatacccag cttgatggag gaaagacttt ccattgacag
     6661 tgccaagaaa tccctcattg ctgtagggag cgggttgtaa ttcttcgcta tggtaagttt
     6721 ggaccatgtc caatcctgcc ggttttagtg gattccaccg aaatcctttt ttcacgcttc
     6781 cttgttcaag cgcagaaaaa aaatcggatg cttcttcgcc taaaagaaga cgatattttt
     6841 tgataaattg ctgtggtaac gttgtcgctt cctccttcat tccaatgttt cgttagacat
     6901 tttacaatct tttttacgaa aaataaaggt ggctgatcta ctttttcatg ttgctgccta
     6961 aggtgactgt tcctttaaga tattttccaa gtattcatcc gtcacctcgt ttttatccca
     7021 aacagcgttc atcatttcaa gaaaatcttg agaagaatca tctgagcgca aacttcccaa
     7081 tgcattaagc cataccaatt caagcgactt ttcttctttg atcttcgtgt gacctttgac
     7141 atatgagaaa aatggccgct taaatagcga atccatgtct aaatcagatt ttgcaatgat
     7201 ttcacgaata ctttcatgga tagaaaaaaa atctttcaat aagtagacaa tgcttaaaac
     7261 atcatcggaa ctgcattcag ctaagtagtt ttcaaaataa acagtgattt cttctaatat
     7321 cttctctttt aacccgctga ttgatttgaa atgtgtataa atcgggtatg tagaacaacc
     7381 gcagaactct gctaactctc ttgcagtaat tgacttcatt ccatattctt ccacgtatac
     7441 catacttttg aacagcaaat attctttact ataatttgtt actctcccca tcattcttac
     7501 ctccgtctct tttcatctac ccataaataa ataacataaa taattctaaa aatattattg
     7561 ataattatca aaacatattt a
//
LOCUS       DACENJ010000125         7536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.129, whole genome shotgun sequence.
ACCESSION   DACENJ010000125 DACENJ010000000
VERSION     DACENJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.129"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..2192)
                     /locus_tag="IW748_001902"
     CDS             complement(27..2192)
                     /locus_tag="IW748_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1104810.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2452..4746
                     /locus_tag="IW748_001903"
     CDS             2452..4746
                     /locus_tag="IW748_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1104811.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFKLLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            4739..5404
                     /gene="pgmB"
                     /locus_tag="IW748_001904"
     CDS             4739..5404
                     /gene="pgmB"
                     /locus_tag="IW748_001904"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1104812.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFTKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            5397..6404
                     /locus_tag="IW748_001905"
     CDS             5397..6404
                     /locus_tag="IW748_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1104813.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            6426..7445
                     /locus_tag="IW748_001906"
     CDS             6426..7445
                     /locus_tag="IW748_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1104814.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         2348 a         1648 c         1412 g         2128 t
ORIGIN      
        1 caggacagct ttttcacaac ctctccttac attttaatgg agccgatcac tttaccagct
       61 gtcactgtac ctaattgctt gatcactact tcttctactt tatcgtttgt aaatacgatc
      121 atgatcgttg gatctttttc ttcgttttgg atcacatcaa gatccatctc agctaatttc
      181 gttcctgttc ctacttcttg gccttctttg acagcaatct caaaagctgg ctgattcatt
      241 tggactgtat cgattcccat atggatcaaa acttcgatac catctttcgt ttccagccca
      301 attgcatgtt tgctagggaa aatacttgtc acttttccag cgatcggcgc aacaacttct
      361 cttgatgttg gaataacagc aaagccgtca cccatcattt tttgtgagaa taccggatct
      421 tttacttcag ataaaggaat cacttctcca gaagctgcgg aaactagttc ttgttctata
      481 cctaagtatt gcttactacc tgcttgtcca tttcctgtag aaaccatttc tggctctgga
      541 atctctgctc ctgattgcag cagatcttcg atatctgatt tcaagacatc tgcttttggt
      601 ccataaactg cttggactcc attgtctttg acgatcaatc ccatcgcccc tgctcgtttc
      661 caggcttctt cagtccctac tttttctcga tcatttacac ttacgcgtaa ccgagtcata
      721 caagcatcga catctttgat attttctttt cctcccagca aatgaatgat ctggaccact
      781 tgctgatctg ctgttcctgt tccgctgtta gatacaacgg tttcttcact gtcattgtcg
      841 taatttccgt tacgtcccgg tgtagcaaag ttgaattttt tgatcaagaa atttgctaag
      901 aaatacgtaa caacaccaaa agcgatcaca cacaacacga agttgataag atctccacct
      961 agacccgctt tgatagctaa cggtgtccga gtcagcaatt cgatattccc aaatgaatgg
     1021 acccgcaatg gcaagatatc tgccatcgca aatgcagccc cttggatcac tgaataaatg
     1081 acatataatg gcacagcagc aaacataaac atgaactcca atggttctgt tacacctgtt
     1141 agaaagacag ccaacgctgc tgaaaaatac attgatttat atcttgcttt cttatcagga
     1201 tcgacattac gatacatcgc aaaagccatt cccatcaaga tacctgaaga accaatcatt
     1261 tgtcctactt tgaaacgagc cggcgtccag ttatgcaaca caaactcata ttgcgacata
     1321 tcccctgcac ctttaagatt cactaaatca gtggcccaag ccaaccataa cggatcttgc
     1381 ccaaatactt gtgtccccgc ctgtgcgcct gataaaatct catacgatcc gccaagctgt
     1441 gtatagttga taggaatcgt cagcatatga tgcaatccaa atggcaatag taaacgttct
     1501 aatgttccaa ataaaaacgg tgctagaatc ggtgcgcttt cttgtgattc tgcaatccat
     1561 aaaccaaaat tattgattcc agcctgtacg tttggccaca ccacagcaag aatcaatgcc
     1621 acgattgttg accataagat aacaacaaac ggaacaaagc gctttccatt gaaaaatgac
     1681 aaagcatccg gcaattttct ataattatag tacttattat aagccattgc cccgacaaat
     1741 ccagcgatga tccctacgaa tactcccata ttcaaagctg gtgcttccaa tacacttgta
     1801 aaaaatcctt taaccatgat cttcgtaccg aacagagtgt gggtaaatgc atcttcattc
     1861 gcaagcatgt cacttgtgac accaaagata gccccagtga tccgattgat taaaataaat
     1921 gagatcccag ctgcaaatgc tccgcctgct ctttcttttg cccaacttcc tcctattgct
     1981 aaagcaaata aaatatgaag attaccaata attgcccaac caatattttc aattactccg
     2041 cccgttgtga caagcggcgt ccaatcagga ttgatcaagg gaattgattt ccctatactg
     2101 atcatcaacc cagctgccgg cataactgct accacaacca ttaaagcctt tccaaatttt
     2161 tgccaaaact caaaactaaa tatctttttc attccgttta gctcctttac gcaatcggtt
     2221 tcgttaaacg tattatagcg tgcgttttct tttttgtaaa tgctttattt tttatttttc
     2281 tgtctatacc attctataaa acctataata cgtgtgttcc cttctataat tttctcctaa
     2341 aaatttattt ttttaagctt ttttctatga aatattttct tttgtctatt gacttgtgca
     2401 atcggttgcg tttattatga acgtataaaa cagctcatta ggaggagaaa aatgaaacag
     2461 atcaaacgac tatttgctat tgatccatgg aaaatcagca cgacacaatt agataaaaaa
     2521 aatcttcgac tgcaagaatc tttaacaagt attggaaacg gttatatggg gatgcgcggg
     2581 aactttgaag aaacttactc aggggaccac catcaaggta cttatttggc gggtgtctgg
     2641 tatcccgata agacacgtgt cggctggtgg aaaaatggtt atccagaata ctttggaaaa
     2701 gtgatcaacg ccttgaactt tatcgcgata gatatttcga tcaacgatca gctgatcgac
     2761 ttggcttctt tggaaccaga agacttttac tgggaattag atatgaaaaa tggtgtatta
     2821 tctcgtacat tcacaatcac tacttctact aataaagtac gcatttcctt cgaacgtttt
     2881 ctgagcattg tcaaaaaaga atcagcttat atctatttaa ctatagaaat gcttgaaggt
     2941 agcggaacag tcaaactcgt atcaaaattg gatggaaatg ttcaaaacga agacagcaac
     3001 tatgatgaac atttttggaa tgagattgat cgtggaacta tagaagaaat cggctatttg
     3061 acgactaaaa ctattccaaa caactttggg attgaacaat ttactgttac tgcagcaatg
     3121 cgccatttcg ttaatggaaa atcagtcatc cctgactatc acgaagatac tttagcatta
     3181 catggttgct tgtctgcaga attatctcaa ggtgagacat ggtcagtgga aaaagaaatt
     3241 atcgttctta ctagtagaga tattccagaa gcgcagcaag cagaaaaagc tgtctcagaa
     3301 ttaacgaacc agacaaacaa ttatatccaa gcaaaagaag agcaaacagc ggcttgggca
     3361 gaacgatgga agcttgccga tgtggtcatc gaaggagacg aagaagcaca acaaggtatt
     3421 cgattcaatt tgttccaact attctctact tactacggcg aagatgaacg attgaatatc
     3481 ggccccaaag gattcacagg agaaaaatac ggcggagcca cttactggga tacagaagcg
     3541 tatgctgtcc ctctttatct tgctttagcc aaacctgaag taactaagaa tctgctgaaa
     3601 tatcggcata atcaactgcc gcaagcaatc cacaacgctc agcaacaagg attaaaaggc
     3661 gcgctttatc ctatggtcac cttcactggt gtcgaatgcc ataacgagtg ggaaatcaca
     3721 ttcgaggaga ttcaccgaaa tggcgcgatt gcctatgcaa tctacaatta tacgaattac
     3781 accggcgata cggaatattt gaaaaatgaa ggactggaag ttttagcaga aatcgcacgc
     3841 ttctgggcag accgtgtcca tttttcaaaa cgagcaaata aatacatgat ccacggtgtg
     3901 accggcccaa acgaatatga gaacaatatc aataacaatt ggtatacaaa tacgatcgca
     3961 gcttgggtct tgcgttacac gcgagaaaat tatttaactt atcaaaaaga agctgctatc
     4021 actatcagcg aagaagaact agccaaatgg aaagatatcg cagagaacat gtacttccca
     4081 gttgacgaag aattaggaat ctttgtccag catgacacat tcttagataa agatctcatg
     4141 cctgtatccg atattcccac aagcgatctt cctctgaatc agcattggtc ttgggacaaa
     4201 atccttagat cttgcttcat caagcaggca gatgtactac aggggattta tttcttcaat
     4261 gatactttta caaaagaaga aaagcaaaaa aactttgatt tctatgaacc aatgactgtc
     4321 catgagtctt ccctttcgcc aagtatccat tcgattttag cagcagaatt aggaatggaa
     4381 agcaaagctg tagaaatgta ccagcgaaca gctcgcttgg atttggataa ttacaataac
     4441 gacacagaag acggcttgca catcacttcc atgactggta gctggttgtc gattgtccaa
     4501 ggatttgctc aaatgaaaac tgcccatgat cagttgagat tcgcaccatt tctgccaaaa
     4561 acttggaaag cttattcttt ccatatcaat tatcgcggac gtttattgtt catctcagtc
     4621 acagatgaac aagttacttt caagttatta gaaggttctc ctctagaaat ttacgtctat
     4681 gaacagtctt attctttagc ggatcgtcta gaaataacgc taagaaagga ggaagcaaat
     4741 gtttaaaggg gtattgtttg atttagacgg tgtcattacc gatactgctg aattccatta
     4801 tcgtgcttgg cgaaagcttg gtcaggaaat cggcattacg atcgaccaca cattcaacga
     4861 acagctaaaa ggcgtcagcc gagaagactc actctctttg cttttagctt atggcggaaa
     4921 agaacactct tttacaaagg aagaatttgc agaattagcg aaaagaaaaa acgattatta
     4981 tttagagatg atccagacga tcgaaccaaa agatgtcttc cccggggtcg tgcctttact
     5041 ggattcttta aaagaagaaa aaatcaagat tgctcttgct tctgcaagta aaaacgggcc
     5101 attcctactt gaaaaaatgg gtcttacacc ttactttgat gctattgccg acccggcaga
     5161 agcaacaaac ggaaaacctg cgccagatat cttcctcctt gcagcaaaag ctgtcggact
     5221 aaaagcagaa gaatgtatcg ggatcgaaga cgcgcaagcc ggtatccaag ccatcctctc
     5281 aagtggcgct caaccaattg gcgtgggacg tgctgaagac cttggagagg agatcccgct
     5341 tgtccctgat acgacttttc ttacgatcga ttacttgaaa gaaaagtggc atgaccatgg
     5401 ataatccaga gatctccgtc gtaccagttc cagagactga aataaagaaa atcacattga
     5461 agaacgacta cttagaagtc gttcttctca attacggtgc ccgcctgcac caaatcttcg
     5521 caccagacaa agaaggaaaa tcagaaaatg ttttattgtc ttacgatcgt ttcgaagatg
     5581 ttctaacaga caaaagcttt tttggagcaa ctgtcggacc tgtagccgga aggatccggg
     5641 atgcttcttg gggtgagcat agattggaga aaaactgcgg atcacaccat atccacggtg
     5701 gcacgaaagg ctggtctttc caattttggg aagtagaagt tttcaaaact ccatcatcaa
     5761 taggtgtggt cttttactta aaagatgaat tttctaccta tccaggacca atcactgcta
     5821 cgatcactta ccgtttaaca aaaaacgaat tagagatgac aacctctgct agtagccttg
     5881 ttgaaaccat ctgcaatccg accaaccatg cctacttcaa cctttcagga aatggaacaa
     5941 gagacatcta tgaacatcag ttaacggtct ttttagatgg tgtgcttgaa ttagatcaag
     6001 aaaaactgcc tactggaaat tggacaaaaa aagaaaaact gccaatcgac tttagaaaat
     6061 ccccaacact gcaagagatc cttgcatgtt atccagatgg actagatgat gtatttcttt
     6121 tgcatcatcc taggctttcc aaaacttcct tacaattatt cgagaagcat tctggtcgcc
     6181 aaatgactat agcaacttct aataaaagta tggtattatt ttctacgact ggattcgaag
     6241 ctgatttctc tgtcaatgga aaacagatgc attcaaacta cggattagca atcgaaccgc
     6301 aagaaatccc tgatatcgtt catttcccca aatttggatc gatcaacctc catcctggtc
     6361 aagaacggat cagtcagacg atttatcggt tctctgcaca ataacaagag aaaggtgttt
     6421 ttatcatggc tattaccgta aaagatgtgg cgaaaaaagc cggtgttgcc acctcgaccg
     6481 tctctcgtgt catcaacgat catcctagta tttccgagac aacaaagaag aaagtccgaa
     6541 aaattatgga tgaactaggt tatgttccta acatgactgc ccgcaatcta ggaaaacgca
     6601 tttccagtgc catcggcgta atcttacccc ctcttgattc aaaagagcgg atcggtaacc
     6661 cattttatct tgagatcatg gaagcaatca acgaagaagc gcggaattat gatatgacta
     6721 cagcaatcgc tactgctaaa aattttgaat tgctgttaga gaacgtccag cgtatgcatt
     6781 tacaaaaaca agtagatggt tttattctag tgtattcaga tagtcaagat actgtcattg
     6841 attatttata ccaaaaccac atccctttca ctttgatcgg acagccttat catcatgaga
     6901 acgaaatcgt ttatgtagat aatgataacc agcttctagg taaacaagca acagaatttt
     6961 tgatccagaa tggacataag aatatcctgt ttgtgacaaa cacgactcat gaaagtttgt
     7021 atttcgaacg atatttcggt tatcaaaaag caatgatgat ggctggtctt cctgcgttat
     7081 ccagtgtagt actagagcat ccggaggatt atctgtcctt tgagaaaacg ttgaaagaaa
     7141 acgaagccac agcattggtg gtcattgacg atatcttcgc ccttcgcacg atgcagttgg
     7201 cgcaaatgta tggctatgtc attccagaga cactctccgt cattagcttc aacaactcga
     7261 tcttctcaac attaacacac ccttacctta caagtatcga tatcgatatc tccgaattag
     7321 gacgtgtcgc tatgcaaaag ctgcgcgaat tgatccatga agaattgagc agtggcgtac
     7381 gtttggtcat ccctcatcgc ttgatcaaaa gagagacagt cgttccttta gaaaacccat
     7441 actaaatagc tgactcacta aatacgctga ccatttatcc tatggtcagc tttttatgta
     7501 aaagaagata ctacttctaa cacattgttt atctgt
//
LOCUS       DACENJ010000126         7528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.130, whole genome shotgun sequence.
ACCESSION   DACENJ010000126 DACENJ010000000
VERSION     DACENJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.130"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(328..552)
                     /locus_tag="IW748_001907"
     CDS             complement(328..552)
                     /locus_tag="IW748_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104815.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            752..3520
                     /locus_tag="IW748_001908"
     CDS             752..3520
                     /locus_tag="IW748_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1104816.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3601..4020
                     /locus_tag="IW748_001909"
     CDS             3601..4020
                     /locus_tag="IW748_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1104817.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4032..4502
                     /locus_tag="IW748_001910"
     CDS             4032..4502
                     /locus_tag="IW748_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1104818.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4519..5298
                     /locus_tag="IW748_001911"
     CDS             4519..5298
                     /locus_tag="IW748_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1104819.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5285..6106
                     /locus_tag="IW748_001912"
     CDS             5285..6106
                     /locus_tag="IW748_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1104820.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6123..7172
                     /locus_tag="IW748_001913"
     CDS             6123..7172
                     /locus_tag="IW748_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1104821.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7185..>7528
                     /locus_tag="IW748_001914"
     CDS             7185..>7528
                     /locus_tag="IW748_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1104822.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIG"
BASE COUNT         2513 a         1256 c         1569 g         2190 t
ORIGIN      
        1 ctatggtttg gcgcagattc tatcaggttt gatggaacga agacaacctc ccgacatcaa
       61 gtcaaaagct acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc
      121 aatgtttcta ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca
      181 ataaaaagga tggtgacaac agtacctctt tactctctga aagcatcata actcgttttt
      241 cctcattatg ccaataaatt gttgttatac cacatataag gatttgtaat agctaaaaac
      301 agacaggaaa gttgaaatct gtgaatctta ttttgtatgg aaggatttat agaacgcaaa
      361 aatcaagata aagatgaata aaaatcctgt ttcggctata tattgcgtat ctacgccaat
      421 aaaacgagtt tgttctgtca tagccatata aagcaaaggc aaaaatacaa ctacagcgag
      481 ataagatccg atgatttctc gaaaagtttt ccaggaagta tgtttcctgt ttttatattt
      541 atccgatttc attgaatagt catagcctcc ttattctttc atatgttgaa attccagtat
      601 aaaaactgga aggaactgta cgttttttag aataaaaatt ataagggata agtaggctgc
      661 tgtctataat aagatatatt tttcaattct ttattaaaga ttttattgac aaaatgaatc
      721 cgttttctat gatggaggag gggtgaataa gttgttgaag aaggaaattt tatgttactt
      781 agagaaccag acagcctttt tcgatttaga acatatgaac gaggtcttta cagcacaaag
      841 gttagctgat cattttgggg taaaaagaaa cacagtcagt cattatttga accagttaaa
      901 tgatgagcaa ctactagtca aaatcaattc tcgaccagtg atttatttcc acaaagaagc
      961 ttttgaaaaa caatttttta agttacgcac gaatttttat ttttctatcg atgatctcaa
     1021 agaggaacag ccttttttcg cgcaaccaaa agacttattc tcttttatga tcggatacga
     1081 cgccagtttg aaagaaagta tcgaacaaat caaaacagct ctttactatc cagatggcgg
     1141 attaccactt ttgattacag gcgaaagtgg cacaggtaaa agttatttag ttaatttggt
     1201 ctatcagtat tgtctgcttc atgatctgct agaagattct gcaccattca tcacttttaa
     1261 ttgtgcacaa tatgcagata atcctgaact tttgactagc aatttgtttg gacatgttaa
     1321 gggagcgtac actggtgcag aagaaaataa aaaaggggct tttgaagcag cagatggtgg
     1381 gatcttgttt ttagacgagg tacatcgctt aacaccagaa ggtcaggaaa aactgttcac
     1441 ctatcttgat caaggtgtga tttatcgtat gggagaacct aatttgtcac gccggatcaa
     1501 aacacgtctc ttttttgcta cgactgaaga gattactagt catttcttaa caactttcat
     1561 tcgaagaata cctatccgaa tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg
     1621 gctagaactg atttactctt tttttctcga agaacaaaga aaaatgggga gccctttgtc
     1681 agtcagtgga ccagctttgt cacttttaac aagtcagaaa ttatccggaa acataggaga
     1741 gctgaaaaat atcgtcaaag tttcagcagc aaaagcatat gctgaacagc gtgaacagcc
     1801 tgaaatccat gtcacgatcc atcatttgca aaaagaacta cttgcgcagc ctattactaa
     1861 aaatacagca cagcaaggaa tctatattac gcaagaccag accttagagc aattaatgga
     1921 gaagcaacag cccgaacagc agcgaatcgt taaaagtttt gagcaaatcc taattgattt
     1981 caagaaaaat aactgtctgc tatattcttg cgaaggaaaa ttaaagcaag aagtgattca
     2041 attatttgat tttttgctgt ttgagaccag tagacaagaa aaacacgagc tgctggttta
     2101 ttggacacaa tctatccgag agacctttcg acaaatggaa actgcctatc aaatcaaatt
     2161 cgatggaaac agtgtctatg ccttgagtta ctatctctat cagcgaagca ctatcaaatg
     2221 gttaccggaa gaacatgata ttatccaact aatcaaagaa ttagaacaac aagtttctca
     2281 gtcttatcca tctagttatc actatgtcca acgaattctg gaattaagta agatcaaatt
     2341 agatattgaa gacacaagta tggatcggat tttactgacg atctatctaa aaaaagcgaa
     2401 atggtcaaaa gaaaaacgac tgccaaaagc gatgattgct gctcacggtt atgcaactgc
     2461 tagcagtatg gcaaatgtag taaatcgttt attacaagag gatctatttg aatcatttga
     2521 tatgccacta gatgtcacac cacagcagat tgcttcagaa atccttgatt attgcgaaaa
     2581 cagcgatgtt tcaaatggat tgatcatatt agtagacatg ggatctctga aagaaatcca
     2641 tcagtttttt aaaaaacagt tatccgtacc gcttttgatt ttgaataatg tgacgacacc
     2701 tttagcaatc acagtagggg aatgcctgca aaaaaatgta tcattagaag agatagcaga
     2761 agagactgtt cgacagatcc agccagaatg gcgactgctc tatccagagg aaaacaaacc
     2821 caaagcactc attactactt gcttcactgg aatcggtacg gcggtccact taagtgaact
     2881 gctagaaaaa agcttaccta cggcttgtca gttgaaaatc atcccatatg aatatcagca
     2941 attaaaagat aagaaaaaca gtgacccatt attttccata tacgaagtcg taggaatgat
     3001 tggcaccaca gatcctgata tcaccaatat cgactatctt tcattagaag acctcatctc
     3061 gggggaaaag atgtctgtac tagctgaatg gttggacact actatgaatg cttcagagaa
     3121 ggaaatattc aatcagcgca tcattcgtaa tttttcattg gaaaaagtac tggattccgt
     3181 gactattcta gatacagaga aggtgatggg agaaatcgat cattttatga gagaattaga
     3241 ggtgcagttg aatcgaagca tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag
     3301 ctgtctcatt gaacgattga tccgacaaat gccaatcgaa acctatcaag gattagagaa
     3361 atggaagcag tgtcaaaaag aagggttatc tgctattaaa tctgcgttta gtgtcattga
     3421 agagcattat agtgtcatga tccccaattc tgagattgcc tatatctatg atattttgag
     3481 tgaagataca gagtttctct cgatagaaga agaattttga acataagcaa gttgttgcca
     3541 ctaattttgg catgcaactt gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa
     3601 gtggatagag aaattatctt agcttcccat ggaaaatttg cttcagggat attagattca
     3661 ttagagctga tctatgggaa aaatcattct attactgtat tcgattgcta tactgatgag
     3721 aattttgatt tagcggaaac agtaaggcag ctttttatga ggtacaaaga taaagaaatc
     3781 attgttctga cggatctttt tggcggaagt gtcaacaacg aattcttaca atatatcaat
     3841 cgggatcatg tctatctcgt tgccggattg aatttgccac tattgattga gctagtatct
     3901 cggttggacg ctgaagtaga gacaactgtt ttgatccagc aggtgctaac tgattcaaaa
     3961 aaaatgatcc agttttgtaa cgagcggctc aatcagaaaa tagaagaaga cgaattttag
     4021 gaggaagaaa aatgattgta ttgacaagag tcgaccatcg gttattacac ggacaagtag
     4081 ctttttcatg gacacaaaca ataggcgcag actgtatttt gattgctaat gatgatgtac
     4141 ctacgaatga gatacgaaag acaacgatca aattagccaa gccacaagga gtcaaactag
     4201 tgatcaaatc aattgatgac tcgattgccg cgttgaaaag cggggtgaca gataaatata
     4261 agttattcat cgtagtggaa tcgatcgagg atgcatataa gctggcagaa gcgtatccag
     4321 aaatcaaaac catcaactta ggagggatca aggcaaaacc tggtactcgc agcatcggaa
     4381 aagcggtaaa tattttacca gaagaggagg aacgcttgaa acgattaatt gacaaacaaa
     4441 tagaagtaga aatccgtcaa gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt
     4501 aagaaaggaa gaacctcaat gattgtacaa gcagtcttat taggaattgt tgcatttgtc
     4561 gcacaatcag aatatgcact agggacatcg ttgctttctc gtccgattgt gacggggctg
     4621 tttacaggaa tcgtgttagg tgatgtaaaa actgggatca ttatgggcgc aactttagag
     4681 ttagcattta tcggatcgtt ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt
     4741 gggattttag ggacggcttt tgctattaca gctggagccg gtacagaaac cgcgctttta
     4801 ctaggtttgc cgattgctac gttgacattg atattgaaaa atatttactt agggcttttg
     4861 attccaatta tgaaccataa agcagacaat tatgcagaag aaggaaatta taaaggagtc
     4921 gagcatatgc acttgcttgc cggtatcggt ctttccttga tgcttggtgt tgtggtaatg
     4981 gtttcttata tggtgggaag caatacgatc ggaaatctat taaatatgat tcctgacttc
     5041 gttcaaaagg ggttgtcagt agctacaggg ttgattccag ctcttggttt tgcgatgctt
     5101 gctcgtttgc tgatcaacaa acaagttgca ccatattttt tcttaggttt tgctatagct
     5161 tcttacttag agattcctgt gaccggagta gctatttttg gtgcgatttt agcagttgtg
     5221 gtagtcaata ttatgaatgt ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa
     5281 gaagatgaag aattctgaga tcaaaaacag caaaataacg aaaaaagagc taaatcacgt
     5341 attttggcgt tcttttcaaa tggagttttc ttggaattat gaacgtcaga tgaatatggc
     5401 ttatgtttat gcaatgattc cgattttgaa aaagttatat cagtcgaaag aagaaatggc
     5461 agcagctttg aaacggcatc tagagttttt taacacgacc ccacatatcg ttacactgat
     5521 tttaggaatc acggctgcaa tggaggaaga aaataaggat gatcctgaat ttgatgtgac
     5581 tgcgatcgat agtatcaaaa catctttgat gggacctctt gcagggttag gagattcttt
     5641 cttttgggga actttgcgat tgattgccac aggtgtgggt acctctttag ccatgcaagg
     5701 aaatatttta ggccctatct tgttcatttt gattttcaat attccgcaca ttctgtttcg
     5761 ctatctagca acaggttggg gatatcgctt aggaacgggt tttttgaaaa agatccaggc
     5821 aaacggtatg atggaaagtt tgacattagg cgcgtctatt attggactga tggtcgttgg
     5881 agcaatgact gccacgatga tcaccatcaa tattccttta aaaatcggtt caggagaaaa
     5941 tgctgtgact gttcaaagta tttttgatga tatcgttcca aatatcttat gtcttggagc
     6001 atttggcata gtgttttatc tattgaagaa agaagtgaaa ccattaacga tcttgatcgg
     6061 actagctttc tttggaattt ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga
     6121 aaatgtttac gatgcaagat tatatctatg aagaaaagga agtactaagt acgatcttga
     6181 aaaagaatga tttttcgaca agagaacata tgaaaaaaac agtgaattta ctgatcttag
     6241 caacgggttc atcttataac gcttgtttag cagcgaagcc agcattggaa tcttatggtg
     6301 atcttactgt cgatattcaa gaaccttttt attttttcaa ttatggaaaa ctttctcctt
     6361 caatcgatac tgtgattgct gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag
     6421 taaaaatgat acaaaaacaa gggttacctg ttgttgctat cacaaatgac gtgcaaagtc
     6481 cacttgcttt agaagctgca caaattattg atttaggtgc cggggtagaa tcagtaggct
     6541 tcgttacaaa aggctattct gcgactgttt tacagttact actcttaggg ataggaattg
     6601 gaataagtaa aaataaaatc agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca
     6661 aaataatcaa ccatttacca gcgattattc aaaaaacgga agaatttttt gatggatatc
     6721 aaagtttatt tcgtttagca cagcgtttcg tcaccattgg atatggtcca aactggggaa
     6781 cagctaaaga agcagagacg aaattgactg aaacaatccg cgttccgtca caaggatttg
     6841 agttagaagc ttatatgcac ggcccatacc tggaggcaga tgcttcgcat ttgctattct
     6901 ttattgaagg ggaaagtgta aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc
     6961 ggtacgttgg agagactctc accattacca caaaaaaagc aagaaacgag aaaacacttg
     7021 gattagcaat agaatgtgat gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac
     7081 tattcgctta taaaaccgca gtagctaaag gtattgactt gaacaaaaag atttttgaag
     7141 actttgatac agtcttgaaa agtaaattat aagaggtgga aatcatgctg aaattcaatg
     7201 aagaagaaca aatcaaagca ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa
     7261 tcatcgataa actttatgcg gaaaaatttg acgctgtcta ttatctagga atcggtggaa
     7321 cgtatgcttc ctcgatgcaa gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct
     7381 ttgtacagca tgctgcagaa tattacacta ctggaaacaa aagattaaca aaagattcta
     7441 tcgttatttt gtcctctgtt accggtacaa ctcaagaagt agtgaaagca gtagaacaaa
     7501 tcaagaaagt aggagcgaca ctgattgg
//
LOCUS       DACENJ010000127         7373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.131, whole genome shotgun sequence.
ACCESSION   DACENJ010000127 DACENJ010000000
VERSION     DACENJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.131"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..563
                     /locus_tag="IW748_001915"
     CDS             <1..563
                     /locus_tag="IW748_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104823.1"
                     /translation="SALAVFLCILFFHLTQRGIPMIAALSAVFSLRQDLTTTVSFGKS
                     RIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVSDGINNNSGIISAIA
                     TLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPETEKKEELSEDLVELQ
                     KKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(651..2150)
                     /locus_tag="IW748_001916"
     CDS             complement(651..2150)
                     /locus_tag="IW748_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1104824.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(2166..2690)
                     /locus_tag="IW748_001917"
     CDS             complement(2166..2690)
                     /locus_tag="IW748_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104825.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            2988..3737
                     /locus_tag="IW748_001918"
     CDS             2988..3737
                     /locus_tag="IW748_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1104826.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            3728..4513
                     /locus_tag="IW748_001919"
     CDS             3728..4513
                     /locus_tag="IW748_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1104827.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            4482..5198
                     /locus_tag="IW748_001920"
     CDS             4482..5198
                     /locus_tag="IW748_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1104828.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(5300..6319)
                     /locus_tag="IW748_001921"
     CDS             complement(5300..6319)
                     /locus_tag="IW748_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1104829.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(6338..7366)
                     /locus_tag="IW748_001922"
     CDS             complement(6338..7366)
                     /locus_tag="IW748_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1104830.1"
                     /translation="MTDMSIVSAITSTVFIILLGFVCRKKGIFSESVGKILSKVVLTV
                     ALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVL
                     TIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAM
                     FVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAG
                     LSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPV
                     NFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQM
                     IHGIGLFS"
BASE COUNT         2206 a         1503 c         1325 g         2339 t
ORIGIN      
        1 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
       61 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
      121 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
      181 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
      241 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
      301 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
      361 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
      421 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
      481 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
      541 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
      601 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
      661 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
      721 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
      781 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
      841 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
      901 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
      961 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     1021 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     1081 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     1141 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     1201 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     1261 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     1321 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     1381 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     1441 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     1501 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     1561 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     1621 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     1681 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     1741 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     1801 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     1861 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     1921 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     1981 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     2041 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     2101 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     2161 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     2221 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     2281 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     2341 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     2401 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     2461 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     2521 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     2581 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     2641 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     2701 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     2761 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     2821 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     2881 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     2941 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     3001 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     3061 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     3121 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     3181 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     3241 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     3301 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     3361 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     3421 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     3481 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     3541 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     3601 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     3661 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     3721 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     3781 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     3841 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     3901 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     3961 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     4021 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     4081 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     4141 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     4201 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     4261 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     4321 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     4381 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     4441 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     4501 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     4561 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     4621 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     4681 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     4741 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     4801 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     4861 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     4921 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     4981 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     5041 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     5101 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     5161 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     5221 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     5281 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     5341 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     5401 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     5461 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     5521 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     5581 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     5641 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     5701 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     5761 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     5821 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     5881 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     5941 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     6001 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     6061 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     6121 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     6181 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     6241 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     6301 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     6361 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     6421 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     6481 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     6541 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     6601 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     6661 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     6721 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     6781 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     6841 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     6901 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     6961 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     7021 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     7081 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     7141 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     7201 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     7261 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     7321 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gat
//
LOCUS       DACENJ010000128         7318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.132, whole genome shotgun sequence.
ACCESSION   DACENJ010000128 DACENJ010000000
VERSION     DACENJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.132"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            240..869
                     /locus_tag="IW748_001923"
     CDS             240..869
                     /locus_tag="IW748_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104831.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(993..2102)
                     /gene="ugpC"
                     /locus_tag="IW748_001924"
     CDS             complement(993..2102)
                     /gene="ugpC"
                     /locus_tag="IW748_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1104832.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2311..2733
                     /gene="mgsA"
                     /locus_tag="IW748_001925"
     CDS             2311..2733
                     /gene="mgsA"
                     /locus_tag="IW748_001925"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1104833.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            3056..3592
                     /locus_tag="IW748_001926"
     CDS             3056..3592
                     /locus_tag="IW748_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1104834.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(3632..5395)
                     /locus_tag="IW748_001927"
     CDS             complement(3632..5395)
                     /locus_tag="IW748_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104835.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(5398..7113)
                     /locus_tag="IW748_001928"
     CDS             complement(5398..7113)
                     /locus_tag="IW748_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104836.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         2198 a         1398 c         1442 g         2280 t
ORIGIN      
        1 gagttagttg gtcttattac ttgatgcaag cttttgacca ctattatgcc gaacaaggag
       61 atgtgacaca aattttgtca catctcctat ttttgtttct tgatcaaaaa attcttatac
      121 ttgatactca cactcatttt gtatggattt agaaaagaat atgtgtcttg acagttatta
      181 gtaagtgagt tatactaagt cgacatttat gtgtcaagac atattaggag agagaaaaaa
      241 tgagaaaaaa ttcagttcga aaactaacga tttctgcttt attgattggg atgggggtca
      301 ttatcccaat ggtcatgcct aaaatcatga ttccaccagc ttcctttact ttggcgtctc
      361 atgtgccatt atttattgcg atgtttttta caccaggagt tgcagtggct gttgcgctag
      421 gtacgacatt tggtttcttt ttaacaacgc cggttatcat cgcattgcga gcattgtcac
      481 atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt atcgttttaa
      541 aaaatggaca gtttactttt gctaattggc gtttccaagg gttcaatttt gtgatcggtg
      601 tgattcattc tttagcagaa atgctagtag tcagcatctt ttactttatt ggtaatatgc
      661 ctgaaacata ttattctcaa ggatattttt atacagtatt catcttgatg gggattggtg
      721 ggcttatcca cagtttagtt gattataata ttgcttattt tatagcagga acactcagca
      781 aatattttga tatccccgta tttacggcag caaaaaaaga aaatcggaaa gagtcgattc
      841 aagggtacgc gaagaaaacg acatattaga catgatctct caaaaaaaaa actctcatct
      901 agtaatggat aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa
      961 catcttctgg cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt
     1021 tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt
     1081 ttatggaaat ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa
     1141 agcatcgttt cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca
     1201 ggtgaagctt caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata
     1261 acttcttttc cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga
     1321 agcttaagcc catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc
     1381 atggcaggag atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct
     1441 ttcggtgtac cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata
     1501 gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga
     1561 agtttggcaa tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc
     1621 tcatccatta agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg
     1681 cgttgtccac cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc
     1741 tctgctgcat tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag
     1801 ccaaaagcca tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc
     1861 atggcaatat cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt
     1921 tctccttcag agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa
     1981 ccggaaggac caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt
     2041 acagaataat tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc
     2101 ataatacgtt ccacactttc ttttttagtt ttattaacta tttaaagtat aaatgaaagc
     2161 gttaactaac ttcaatgtca tgttgaacaa gaattttttc ttttttttgt caatgtgcta
     2221 gttttaagct ttaataaata tccataagaa ataaaaactg atacactgga gtaaataagt
     2281 atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga
     2341 aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa
     2401 ctttttgcta ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat
     2461 cggtttaaat ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat
     2521 cagttagatt tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat
     2581 gtaacagcac ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga
     2641 acagctgagg tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc
     2701 catgaaatag acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt
     2761 taatgttcgc ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac
     2821 gaacgtattg gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa
     2881 atatgctata gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg
     2941 ttaagtgcta agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt
     3001 attttcgcat tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa
     3061 gaaacaccgg ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt
     3121 cactttgtcc cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat
     3181 tccacaagtg atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt
     3241 agctgattta gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt
     3301 gaatgcattt attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg
     3361 gaaaaatgcc attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc
     3421 attatggctt gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg
     3481 cctgttaaaa acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg
     3541 tagtattcct tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat aaaaaacaag
     3601 cagtcttccc atcccagaag actgcttgtt tttattgtgc tgtgaattgt gcttgataga
     3661 gccgtcggta ataaccatct ttttgaagca gctcatcatg ggtgcccatt tcgacaatag
     3721 ctccctgatc catcactaat atctggtccg catttttgat tgtggacagg cgatgggcaa
     3781 tcacaaaact ggtttttcct tccatcattg ctaagaaggc ttgttggatt tttttctctg
     3841 tcaatgtatc gactgagctg gtagcttcat ctaagatcaa cataggggga ttactgatca
     3901 tcgttcgagc aatcgtcaac agttggcgtt gtccatctga tatctttaac ccttgaccgc
     3961 caatcttagt atctaatttt tccggcaaac gttctacaaa ttcatacata taagaggctt
     4021 ttagggcatc attgatttct gcgtcacttg cttccggatt cccaaaacga agattttctc
     4081 gcaatgtact gtcaaatagc caagtatctt gtaacaccat tccaaaactt ttacgtaatg
     4141 tatcacgttg gatagagcgg atgtcatggt catcgactgt aattgttcct tttgttgtgt
     4201 cataaaaacg catcagcaaa ttaacaagtg tggattttcc ggcccctgtt tttcctacta
     4261 tagcaatggt ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa cgctgcttgg
     4321 ggtcataaga aaaagaaaca tgatcaaaag agacttttcc tttgatattt ttgggttcca
     4381 aggcataggc atctggcaaa tcaggctttt cttctggctg atcgatcaaa tcaaatgctc
     4441 gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt tcgataaatg
     4501 gtttagaaaa ctgacttgag tagatcgtaa agctggaaat cacaccaaca gtgattgacg
     4561 aaccactctt caatgccaat aggccgccaa ctaaaccaat cgataaataa gcaaggtgat
     4621 ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt tgtccttttt
     4681 catatagatc agcatttagc agttcaaatt gctgttgact agcttcttct tgctgaaaag
     4741 ctttcacgat tttctggtta ccgattcgtt cggtcacaaa gcccgagata tccccaataa
     4801 tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa ctcacaagga
     4861 aaataattgg tgtagaacaa agaacgacca atgtcagaag tggacttaga cgcatcataa
     4921 aaataaacga aagaatgatg attgccactc cagaaaataa ttgattgaag gccgcagaaa
     4981 ttgcaatgga aatattgtcc atatcatttg taaatcgact gacgatattt ccgtgggagt
     5041 tttgatcata ataacgaagt ggcaatcgat tcagatgagc aaatgcatct tttcttaatt
     5101 ttgcaacgga taagtaagct aaccgattgc ttaacacttg gatgagccat tgactgacta
     5161 cgttgattaa aacgatacca ccgaatagaa taagtaaatg gaacagtcga ttgaagcgga
     5221 cctgtccttc tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc atcacaactg
     5281 ttgcaatccc attgatgatt cctagtatca cagctagcca aatgtctttt gcataggcag
     5341 taaggtaagg aatgaatcgg cggagcgtct gcaaggataa tttttttact ttcataacta
     5401 ttcttcctcc tgtgatgcga cgatttcctg atattctgct gattgagcaa gtaattcttc
     5461 atgggtaccc tgagcagcaa tcttaccttg atccatcact aagatatgat gtgcttcttg
     5521 aatagagcgg acacgttggg agataataat cactgtactg ccaagataag tctgtagcgc
     5581 cttacgcaaa tccaagtctg tttgataatc caacgcactt aaagagtcat ctaaaatcag
     5641 taatgctggc ttggcaatca aagctcttgc aatcgtcagt cgctgttttt gtccgccaga
     5701 gaaatttttc ccattttcca tgacttttgt atctaaacca tttggaagct gacggacaaa
     5761 ttcttctgcc tgagcgattt tcagagcttc ccaacattct ttttctgtgg cgtctttctt
     5821 accccattga aggttttcac gaatagtccc ggtaaatagt acagaagttt ggggaacaag
     5881 agcaatttgc tgacggagat gatccagctg ccaagaacga acatccattc cgtccattat
     5941 aactgtaccg gaactgacat catagaaacg cggaatcaat tgtgtcaaag tcgttttccc
     6001 gcttccggtt ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa atgtaatatt
     6061 ttggagagaa agaccagaat caggagtata acggaagtca acatggtcaa aagcgataat
     6121 tgcttcagaa gaaaaatcgg gagtagtact gacctcagct tcaagaatcg aattttctgt
     6181 gtctagcacc tctttcaccc gatttcctga agcttcggca cgtgtgaaga tcacgacgag
     6241 gtttgaaaca acgatcagtg ccaaaagcat ttgattcata taattgatca aagcaagaac
     6301 ttgtccttgt tgcaagctac caatatttac tttgatcccg ccaactgtca gcaaacagat
     6361 gatcccgaca ttcatgatca aggttgtagc aggtgataat agggcagaga tattggaaac
     6421 acgtaaataa ttgttcgcta gatcatttgt gcttttatcg aacttctctg tttctttttt
     6481 tgtctgcgca aatgcgcgga tgacacgaac accgctaaga ttctgactaa cgtgttcatt
     6541 gagctgatct agtttttctt gcactttttg gtagagagga acggtttttt tgatgataaa
     6601 aaataaaatg attgagaaaa taggcaataa tgctaagaaa aacagaccta cttcccagtc
     6661 gatcacaaaa gccatgacca ctgacccgat acttaaaaac ggtgctctga tcaataaacg
     6721 aatgaccatt gctaaagcta gctgaagctg attgatgtcg tttgtcattc gggtgatcaa
     6781 ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt ttttgattag
     6841 ctgattacgt aactctgtac caaatccttg tgaagctact gaagcatagt attggcaaat
     6901 gatagcacaa atcagaccaa tcacagacat agcgaccatc cataagccca tttcaatgat
     6961 ttttcctcgg tcattcattt ttagtccatt atcgatcaag gaagccatca ttaaaggcag
     7021 gacaagttcg aagacagctt caagaaattt gaagaacggg cccaaaatga tttgcttacg
     7081 atagtctttt gcataacgta ataactgaat catatgattc ctcctattat ttatttgtcc
     7141 acaattgaaa aaacaagtac taccacattg tagcttagga aatttagaat gaaaagagaa
     7201 caagcctttt cataaatgaa agttttatta tgaagaagct ttagaaattg aatagactat
     7261 tttttttaaa aaaaagaaaa tgagaaaaca tgccagtctc ttcattcata ggaacgtc
//
LOCUS       DACENJ010000129         7286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.133, whole genome shotgun sequence.
ACCESSION   DACENJ010000129 DACENJ010000000
VERSION     DACENJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.133"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(15..839)
                     /locus_tag="IW748_001929"
     CDS             complement(15..839)
                     /locus_tag="IW748_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1104837.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(812..1492)
                     /locus_tag="IW748_001930"
     CDS             complement(812..1492)
                     /locus_tag="IW748_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104838.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1515..2453)
                     /locus_tag="IW748_001931"
     CDS             complement(1515..2453)
                     /locus_tag="IW748_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1104839.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2698..3549)
                     /locus_tag="IW748_001932"
     CDS             complement(2698..3549)
                     /locus_tag="IW748_001932"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1104840.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3703..6276)
                     /gene="mprF"
                     /locus_tag="IW748_001933"
     CDS             complement(3703..6276)
                     /gene="mprF"
                     /locus_tag="IW748_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1104841.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6417..7223
                     /gene="proC"
                     /locus_tag="IW748_001934"
     CDS             6417..7223
                     /gene="proC"
                     /locus_tag="IW748_001934"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1104842.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         2226 a         1602 c         1257 g         2201 t
ORIGIN      
        1 cttttcttta taatttagtt attttttctt ctgaaggcaa ccaatattcg tttgatgata
       61 tttacaatca ggaacaaagc aataaagact aatgtgattg ttgctcctgg aggtgtatct
      121 agataaaacg agctagttag tcctgacagc atccctagaa atcctacaca aacactgata
      181 gcaatcacga cattgaagct tttgcctaat cgcataccga ttgctgcggg aagaaccatg
      241 attgcggata tcaaaagtgc accggcaatc gggatcatca cagcaatagc cacaccagta
      301 atcacattga acatcatcga catcaaatga acgggtagcc catccacgtg ggcggtatct
      361 tcatcaaaag tcagaacata catcggccgt ttgaataaaa agaacaatat accgatggca
      421 acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac
      481 agataggatt ggatacttgt tgctgaattc cctccgctca aattcatcaa tactaacgct
      541 agcgccaaac caccagccat taaaatggca atggagattt ctgaataggt tcggtagatc
      601 gttcgcagat actccaatat cacagctgct aatacaacga ctagcatggt cgtaagtgta
      661 ggattaaggt tcaaaaagaa tcctaacgct acccctgcta aagagacatg agaaagcgta
      721 tcagccatca gcgattgtct tctgatcact aaaaacacac ctagcatcgg ggcgattccg
      781 gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt catatgaaag caacgccatt
      841 gagaatcctc ctttctcacc agtcggatct gtctgtccgc atactgtttg atatcttcat
      901 gatcatgggt gatcattaga actgatttcc catggacatg cgcactatgt cggagaagac
      961 gataaaattc atttctggat tcttcatcca tacctgttgt tggttcatca agaacaaata
     1021 agtcagggtc agtagcaaaa atacgcgcta aactgattcg ctgcttttgt ccgccagaca
     1081 attcacctat tcgccgattt cgcatatccc acatccctac agcttctaaa gcacgctgta
     1141 catgttgatg atctttttca gagagacgct tgaaccagcg gcctcttgga tatcttcctg
     1201 aacggactaa ttcgatcacc gtactaggaa aacctgcatt gaaagatgca acttgttgag
     1261 gaatataacc gatacttaat ttttttcctt cttgattttt ctcagcaatc tttatcattc
     1321 cttttgaagg tttcagtaat cctaacgatg ctttcaccaa ggttgacttg gctgctccat
     1381 tttctccagt taaaatgaca aattcacccg catctacatg ataagagaca tcttctagta
     1441 caggttcatc atcatagtaa aaggtcaaat cagatatttc gatataacgc accttacaac
     1501 ctccattcta aaacttaatt gatactttct tttaaagctt tcagattttt ttccatgaca
     1561 gaaatataat tttcgcctgc tttgatttgt tcattagtca agctttctaa aggattcaac
     1621 acttctagct tcactcctgt ttcattcgac aatgtctcag ctaccttcga agaggcattt
     1681 tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa attcttttag ctcagctaag
     1741 cgacttggtg tgggttcctg atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg
     1801 tattgattcg ctaaataagc aaaagcagca tgctgagtga caaatgtgcg gtttgttgca
     1861 tcttttagct catcggagta tttttgatcc agtgcttcaa gctttttgat ataagcatcg
     1921 gcattcttcg taaaagtttc ctttttatct ggatattttt cgcctaattc ttcagcaatc
     1981 gttctgactt cttttatagc taataccgga tcgagccaaa catgtggatc gtattcatgt
     2041 gaatgaccat gcccttcttc tgattcttca tgttcgtctg cttcttgcga ttctaaacgg
     2101 tcgattcctt tagctgcttc gatcatcaag gtctgtttgc tatccactga atcctttaac
     2161 gaatccacaa aaaattccat gtctgaacta ttatacacga aaacatctgc atcggaaatt
     2221 tctgctcttt cttttgcact tggttcgaaa tcatgcggct ccgttccagc agggatcaaa
     2281 agtttcacgt ttccttcatc ccctacgatc tcttttgtaa aatcatacat cggataaaaa
     2341 gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct tgttcgtatt tccacatgct
     2401 gcaaacagta aagcacctat cattaccgta gctgtcaaaa gatatttttt catttatatg
     2461 ctcctttcaa tgaacaaatc ggaattttac gatttattaa cgtgaactaa tgtatcaaaa
     2521 taaaaaaaca cagtcaatag aaatccatca atttctgatt taagcatgta tattcttgat
     2581 taaatcgtaa aatggatgag ttgctgctca tatctagcca tttcactaca ataaaaaact
     2641 gaagaatacc attttaggat tcctcagttt ttcatcacct attttacttc ttatatttta
     2701 ttgcagggtt cgtactaaat aaacttcctc acaaaaacct tttaacccat tatatactct
     2761 ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa
     2821 tgcagcatct gctccgtact tgatcatacg ctctttgatt tgatggatga ctggatgccg
     2881 ctttcccgtt atatcttcta aactgtttcc catttttttg atcatctgct gataatcttg
     2941 ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt tggatggctt gcgtatctac
     3001 ttctctaaac actgtacgtg tagagacact cattcttggt ttgaccaaca caacccagca
     3061 ctgtggcata gaaggtaata cttggatttc ttcccctttg ccaccgacaa atgccgtact
     3121 tccgtagaca cagtaaggaa catctgtacc gacttccaaa ccgatttctg ccagttcttc
     3181 taacgaacag cccagattcc ataattggtt gatccctctt agagcagctg cacagtctgt
     3241 acttcctccg cctaatccag ctgctactgg aatattcttc ttaacaaaga tcttgacacc
     3301 ggttttgata ttgtatttat tttttagcaa taaagcagct tgataaacat tgttccgttc
     3361 atctaccggt aaaaatgctt tatttgtttc tacaatgatt ttgtcttctg ttatttcttc
     3421 aaaaaataga tgatctgcca agtctacgct cgacatcacc atttccaact catgataccc
     3481 atcttgccgt ctatataata catccagtcc taaatttatc ttggctggcg ctttttcaat
     3541 caattccatc ctctcaccca ttcgaaaagt ctaagtcgca agtattctat catagggaaa
     3601 aataaaagta tagcttatgt ctgcctagtg gttgattttg tcatcgtttc aacagctttc
     3661 acagaatttt ctcttcataa tccaaacaag ttctagtgat tcttataatt ctgtgagcac
     3721 ttcttctgcc aaactcggaa catttccttt ttcttttcct atcagactga gtagctgcat
     3781 ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa taacgtcctt cccaatgatc
     3841 gacatatttt aatttctttt cataagcaga ctggaaggaa tagaaaaaat taccataatt
     3901 atatacgata tgcatgactt tttcttgaaa gaaagaaaaa tctgtttctc caacattagc
     3961 taatggagct aagcctagat tggcttccga gtagcccatt ttctcgtata caataaacag
     4021 attggctaac agatagtcag ctaattcctc agattcttta ggtaagaccc gcaacaaatc
     4081 atatcccgcc tgcttcttcg tcatcggctg agcggtgatg aagccaacga ctttttgatc
     4141 tttacggaca ataccgacac cgctagataa tacatatgat ttatcaaatc gtcccacaga
     4201 gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa acttcattta cagcttgatg
     4261 taattcttcc ggcagttgat cgtaccaagt aaaatggtat ccttctttca tcaaacgttg
     4321 atattcaagt tgctgactca gccgagacaa gtcttctgtt gctagatcaa caatccctgc
     4381 ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc atcactacgt agctttctga
     4441 tatcttataa aacgcaagct gatagcctaa acaatctgct tgtttcataa actgattcgt
     4501 tgctgcccgc atcattttag gattgccaac tggatctcct aaaacaaagc atttgtttcc
     4561 tttgatctga taaccaaaaa cgacttgatc ttggtcgtct tcttggtaat aaaaataact
     4621 gtaacctgac aagcgaagct gatggcttgt agctgtgcct ccgtatgttt ccaataaacg
     4681 gtcaaaacgg tctttttcgt actcactgcc aagcttcgta gtatcttcag ctaaatattg
     4741 ataaagcgcc actaaagcta gcaatgagat gcccaaacca ataagccctg agaaccacac
     4801 atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc
     4861 gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa
     4921 gattatagct ccccaagaat aaacgaattt tgttcgataa aattcttttc tggataacca
     4981 cacagctaac agaatcaaga catataccaa tatcaactgc caagataacg tacgtgtgat
     5041 tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc agaaatgcct ttttgacttt
     5101 tactgaaagt gctctagcaa ggccgagtaa taaaaagcca accaatagat tcactgtctg
     5161 atctaaaaaa ttgaatgaga acggcaataa tacttgaaat agccgactga ttgcagacag
     5221 attagtgatt gttgatagaa gtaccatcat gatccccgca aaatagacag cacttacgag
     5281 cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat
     5341 tttgactcct gtatgtgtga tgaacaatag aagtcccgtc ataaaaggta gaacataata
     5401 aaacaaccga taatataata gccaaactac tgctacatct tgccgaacag acaattgact
     5461 cattcctaag atcatcaaaa cgtcaaaagt ccccattcca cctgggacca tcgtcagcat
     5521 tcctatcaat gtagcaatca tgaacatagg atagatcgaa agcatcgaaa tatctattct
     5581 catcaacttg ccgatgatca aaaataccgc aagggctccc gtccattgtc caaaggatgc
     5641 cagtagcaga ccaagcaagc cttttggata aaactcgaag aacaatgtct ttctctttag
     5701 ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg ccgaaaagcc agatccaata
     5761 gcttcgaaaa atactttctg actgaataaa aaagacatca ataaatgtaa gaaacgacca
     5821 tacagacaaa ccagtcagca taaatagtgc gacttttgat acagtggcta aaaccatttt
     5881 tctatttgtc tgctttccat aaaaactagc ccgcagactt gctcctacca caccaccaaa
     5941 accggcgaga ttgttgatcg tgtttgttac ccacgcagca agaagaaact cttttctttc
     6001 catttttggt tttccttgtt tttctaagac tctgatcgtc acccaatcat acaacaacat
     6061 cggagtgaca cccagcagtc cagccagaat catgagcagg atcgtcatgc ggctttgttg
     6121 tcccatcctt tgccaaatat cttgccaact catgccatta gcaatatttg cgacttgatt
     6181 tgccacaaag actaacacgg agccgaaaaa aatcagtttt aaaatcagac tatgttcctt
     6241 tatccattgc agcatcaagt atcgtttatt tttcacattt tcccattcct aacttgtata
     6301 cttttgtgtc ataccccaat gttatcttat ttattttaat ttttccactt tagacctgtt
     6361 ctttgcttgc gatgatagaa aaagaaagat atcctaatgc taaaggagag gataacatga
     6421 aagtaggatt tatcggcaca gggaatatgg cacaagctat tattgatggt ttattaaaaa
     6481 aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg acattttgaa aatgcacaaa
     6541 aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat
     6601 cagatatcgt tcttttagcc gtgaaaccaa aaatcattga aaaagtattg gaagaactaa
     6661 gagaatatag ccagaaagtc ttattcattt ctattgcttc aggaaaatca ttaacagatt
     6721 tagaaacagc gcttgaagat cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg
     6781 tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga agctgccacg gaggaacagc
     6841 ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt ctatccttta gcagaatcag
     6901 atttcagtac attcattggc cttgctggca gctcacccgc attcgtttac atgctgatcg
     6961 atgcgatggg acggactggt gtactgaatg gtttgtccaa acagacagcc actgagattg
     7021 ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc aagtgataaa aatccatggg
     7081 aacttgtaga ccaagtttct tctcctggcg gtacaactgt tgcaggtgta gttgccttag
     7141 aagaagcagg attcatccca gcaatcatca aaggaatcaa tacgacgatc gagaaagatc
     7201 ttgagatgtt gaataaaaaa taatgatagc aatgtttttt cgtaccttac aaaaaaagaa
     7261 gagctgtggc ttttgccgcg gctctt
//
LOCUS       DACENJ010000130         7242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.134, whole genome shotgun sequence.
ACCESSION   DACENJ010000130 DACENJ010000000
VERSION     DACENJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.134"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..313)
                     /locus_tag="IW748_001935"
     CDS             complement(<1..313)
                     /locus_tag="IW748_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1104843.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MG"
     gene            complement(306..779)
                     /gene="tsaE"
                     /locus_tag="IW748_001936"
     CDS             complement(306..779)
                     /gene="tsaE"
                     /locus_tag="IW748_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1104844.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(902..1882)
                     /gene="pta"
                     /locus_tag="IW748_001937"
     CDS             complement(902..1882)
                     /gene="pta"
                     /locus_tag="IW748_001937"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1104845.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2035..2715)
                     /locus_tag="IW748_001938"
     CDS             complement(2035..2715)
                     /locus_tag="IW748_001938"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1104846.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            2841..3710
                     /locus_tag="IW748_001939"
     CDS             2841..3710
                     /locus_tag="IW748_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1104847.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            3736..4251
                     /locus_tag="IW748_001940"
     CDS             3736..4251
                     /locus_tag="IW748_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104848.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4298..4861)
                     /locus_tag="IW748_001941"
     CDS             complement(4298..4861)
                     /locus_tag="IW748_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1104849.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(4916..5095)
                     /locus_tag="IW748_001942"
     CDS             complement(4916..5095)
                     /locus_tag="IW748_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104850.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5172..5681)
                     /locus_tag="IW748_001943"
     CDS             complement(5172..5681)
                     /locus_tag="IW748_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104851.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(5777..6157)
                     /locus_tag="IW748_001944"
     CDS             complement(5777..6157)
                     /locus_tag="IW748_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1104852.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6487..7236
                     /locus_tag="IW748_001945"
     CDS             6487..7236
                     /locus_tag="IW748_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1104853.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2061 a         1488 c         1293 g         2400 t
ORIGIN      
        1 gtcccattcc ccaatattcc tgcagaatac tgataccgac ttctcccacg tgagacaaac
       61 gaaattgact gtctgccttg acagatgctg ttccaataat cgtcccttca tagattgcaa
      121 gcagcaataa attattattg ctttcgtata tataatccaa ctcttcttct aaggtttctg
      181 gtggtaacag gagttctgct tcatccaaaa ctaaaaattc tgtctcctta ttaacttgtc
      241 ccatcatcaa aagaatgtct tttgcatctc ctggcactgc ttcccgcaag atgatcccaa
      301 cttcttcact catgattttc agcccatttt tctagaattc gctgtttgaa ggtttcactt
      361 tgctccccgt atgagattag tttcacatac cgcttttcta aatctgtatc acttttttct
      421 aaaatcagtt ccaaataatc ctccggcagt gcttcttcta atagatttcc ccattcaaca
      481 acggaaagcc catcaccttc aaaatattca tctaacccta aatctgctcc gctagctgct
      541 acccgataaa tatccatatg atacaacgga atccgacctt gactatattc tctaataatc
      601 gtgtatgtcg gacttttgat catttgcgtt atccctaatc cttgggcaat tcctttcgtc
      661 aacgttgttt tacccgcacc tagatcacca gtcaatatca gattgtcacc tggcattgcg
      721 gaatgaccaa ttgcctctgc gaattgttct gtcattgtta atcctgatag ttcgatcatt
      781 tttcatcttc tcccgctctt ctttcttttt gccagtatat cgcgtaatga agttttttta
      841 aagaaaaaat gaactagtca agaacagcag ctgctgtttt tggctagttc gcgtgtattt
      901 gttaatcaat caatgtttgt gctgctgtga taatcgataa tttatataca tcttcttcat
      961 ttgctccacg agacaaatca gaaaccggtt tgttcaagcc ttgtagaatt ggtccgattg
     1021 cttcaaagtt accgaaacgc tgagcaattt tatagccgat atttcctgac tgcaattctg
     1081 gaaatacaaa gactgttgct tgaccagcta cttctgaatc tggcgctttc agttgtgcaa
     1141 ctgaaggaac ataagaagca tcaaattgca attcgccgtc gatcatgtat tgtggcgcta
     1201 gttttttagc gattttagtt gcttcaacta ctttgtctac ttctggcgat ttcgctgagc
     1261 cttttgtcga gaagctcatc attgctactt ttggttcgat atcgaacaat tctgctgttt
     1321 ttgcagaatc tacggcgatt tcagccaatt cttgcgcagt tggattcacg ttgatcgcac
     1381 aatccgaaaa gacatatttt tcttgatctc ttccacgtac catgatcatc gctccgcttg
     1441 tccgactgat gccaggcttt gttttgatga tctgaagtgc tgggcgaaca gtgtcgccag
     1501 tcgaatggat cgctccagag accattccgt ctgcaatccc catatacgta agcatcgtac
     1561 caaagtaatt cacatctttt aaaatctttt ctgcttgttc tttggatgcc tttccatttc
     1621 tacgttcaac aaaagtatcc accatttcat cccacttatc gtaattatca gggctgatga
     1681 tcgtaaagct agatgttttg attcctctgg catgcgctgc atttcttatt tcttcttcgt
     1741 tccctactaa aatgggttcc acaagttctt ctgctttcaa ccgggcagct gcgcctaaaa
     1801 tacgagcatc agtagcttcc gggaaaacga ttttgatccc acggcgaaca attttaaatc
     1861 ttaaactatc aaacaattcc acaagatgac ctccagacgt ttttatatgt attatcatac
     1921 actattccag aaaaaaagaa cagtttattt tatctgtatc agtcaaaaat gctggaaaaa
     1981 ttaagttttt cacatacaaa gcgaactgca ctataacagg aaagcgctgt tttattacac
     2041 tgtttcaggc agttgccaat caatcggttc ttctcctaac gcaaggagcg catcattcgt
     2101 tttcgagaag ggtttggaac caaaaaagcc acgacttgca gataacggac tgggatgtgg
     2161 agatgttatg atgatatggc gactcttatc gatcattttg attttttctt gtgccggttt
     2221 tccccacaag ataaacacga ttggtttttc ccgttcattt aatttttcaa tgatcgtgtc
     2281 tgttaatctt tcccaacctt tcccgcgatg agaatacgct tgcccttccc gaacggtaag
     2341 aacggtgttg agtaaaagga ctccctgttt agcccatgac actagatttc catgttgaac
     2401 aggtgcgatt ccaagatcat cataaagctc tttatagata tttcttaacg atggcgggat
     2461 ctttacgcca ggttgcacag aaaaagataa gccatgagct tgattttctc catgataagg
     2521 atcttgtcct aaaatcacca ctttaacttc ttcataagga gtcaattcca atgcttcata
     2581 gatatgatac atatctggat ggatcttttg ggacgcatat tcttttttta aaaattctct
     2641 tagatgcaga taataatctt ttgaaaattc tgaggacaaa acttcttgcc aactgttatg
     2701 aatgatttct ttcaaaaact ccacctccta ataaaaagaa taactcacat tttcttcaat
     2761 cacaagggac aatcttcacc ctcagcactt gattaatatg gtaaactagg aacaagaagc
     2821 agaaaggaac gataatcatt atgatcaaat taattgcatc agatatggac ggcacattat
     2881 tagatgccca tatgagtgtt tctcctgaaa atgccgaggc gatacgtttt gcaaatgatg
     2941 ccggtgttga gttcatggtc gctactggtc gaaactatca agaagctcgc gcagctttag
     3001 atgaggtagg aatcgattgt gcgatgatca ctttgaacgg ggcacaggta tttgataaag
     3061 aaggaaattc attgtttacc gtgtcgattc caaataatca agcaatctct gttttagata
     3121 ttctagatgc taacggtatt tactatgaag tagcaaccaa cgatggtctt tattctgaaa
     3181 atcaagcgaa acgaattgaa agttttgctt ccatggtggc tacccattta cctcacctta
     3241 cgtataaaat ggcgattgcc atggcctcag ctaagctgga gttattgcac atcacatacg
     3301 tggacagtat tcgtgctatt ttggaagaaa agaatctgga agttttaaaa attatttgtt
     3361 ttcatacaga agggccagct gtattaggtc cagttggaaa gcagatttct cttttaggcg
     3421 atctagccgt tacttcctct ggtcaaaaca atatcgaagt gaaccataaa aacgctcaaa
     3481 aaggaattgc tgttgcccat gtcgctcacg agcgtgggat ttctttagaa gaagtcatga
     3541 cgattggcga taacttcaat gatgtcagca tgctgcaagt agcaggagtc agtttcgcaa
     3601 tgggaaatgc agaaatcgaa gtaaaagatt atgccaaata tacaacggac acaaaccttg
     3661 aatctggcgt aggaaaagcc atattgcgcg cgattgcaga agacttgtag ttttatctag
     3721 aaaagaggga tatagatgtc agaatttttc attcaggaaa agcagcttgg acgtgcaact
     3781 cggacaattg tcaaaaacga aaaaggacat tccctgtttt tgatggttgg tcgctgggga
     3841 acaaagggcg atgcattgtc tctttatgcc atgaatggcg atctcgttgc tcacatcaag
     3901 cagataagcc tgacattcgg tacgcgtttt gagctgtata aagagttcga aaaggtggga
     3961 accatgcaga aaatattcaa ttggcccggt gacttttatt atattcgtag acttcactgg
     4021 acagtccaag gtgatatcta taatcatcaa tatcaaatcc atcatttcaa tcagcaaatc
     4081 atgaaaatgg ataaagcaac acttttttca ggagattact atgtattgga tgtctcaaat
     4141 gatgaagatg ctccagtttg tatttgtatt gctgcggttt tagattactg gctctaccgt
     4201 aaagacaaaa gtcgcgataa gcctgtttct taccgactta acttaagtta aaaagatgta
     4261 cctgaaatgc ggaaatacgc atttcaggta catcttttta gatattcttt tgcatatgat
     4321 cataaagatg accactgatc atccgctgac cgacaacttt gaagtcatgg cgttcgtata
     4381 attttttagc ctttggattt ttttcatcca cacttagacc gatgacggag cgattagctt
     4441 tcttcgccaa tttaggtaat gcttctaata atcgcgaacc gatgccttgt cctctaaaat
     4501 cttctgatac actgatagag tccaaatacc attcgttggg aaacgtttct ttatcaatga
     4561 acaaacgtgt agacgcatct agctgatatt tctctaagat aggtaccagt ggctgatcga
     4621 tgattgattc ttcttcatct gtataaccgt aagcaactcc tgcaacttta ccgtctattt
     4681 cttctacgat acctcgtgaa taactgtaac gataatttgg atcagtgatt gcttcttcta
     4741 aaatctctaa agtcttttgt tcccctgtct gcttcaaaaa tggcagttcc atatctttta
     4801 gaatcactaa gatcaatggg gcgatttctg gagcatcttc ttttctcgca aaacgaatca
     4861 tagtttcctc tccttttctc cattctaaca tgcagaaaaa gaaatcgcaa aatcgttatt
     4921 ttaaaaaatc ttcaatcttt tcagtaatga aataaccatg agtaacgatt cttgccgctt
     4981 cagttcggca ataagggcag acgactggca tttctttatc attatgttcg actacattat
     5041 tgacttcttc gtatgcaaat ttttttccgc aattagggca tttcattttt tccatgttat
     5101 ctcaacttcc tttcatcttt actctcttag cttactctta tattgtaaaa aagagaagca
     5161 atatccttct tctatgcaga tatccatacc tgacggtcct gttctccatc tattaatcgg
     5221 actttataat aaggacgttc atctttgact agaagtgtcc actctttcac ttttgctttt
     5281 ttcccgtagt agctgactgc ttccttcatc gctgcttgcg gttgtttgaa ctcttccgtt
     5341 ttttctatat caaaaagatg ttctgcggcc ttttccgtgt catttcgtac ggttgttttc
     5401 ccagtggatg gattgactgc tatgacttgt ccttcacaaa aaaactggta ttcatatgtt
     5461 gcattcgtag gattctccaa atctttttca actgcaaaat gaatagcaga tatgttatgt
     5521 tcttttgttt cgttttttac gattgctgct gcttttttca atgagatccg atattcctgg
     5581 tcactaatat catccatgtg aaaagcggca ttgagcagtc ccgtcatacc ggtaatccct
     5641 attacgatga gggcagcaac accaaacatc atttttttca ttcttcattc tcctgtttct
     5701 tttttttaaa gaatatcaag aaatgaacga atgaaaaatc gaatgaaacg aaaaaaaagt
     5761 gacaattttg ttatctttat tcgtgcagtt ctaatggcaa tccgtctgga tcaaagaaaa
     5821 acgtcatttt ttttcctgta aaggtatctt ctctaatcgg ttctgattcg atccctaagg
     5881 actgcaattc tgcaattgtt tcttcaatat cgtctacttt gaaagcaaga tgacgcaagc
     5941 cacaagcttc tggatagttt ggtcgttgtg gcgcattagc aattccaaaa atctccaatt
     6001 cagcacttcc tagtttcaga tctaatttat agtcatctcg ttctttgcgg taattttctc
     6061 tgattacttc aaatcctaat ttattaacat aaaaatcacg tgatttctga taatctgaaa
     6121 caatgatggc tatatgatgg atcgttgtaa ggttcatatg agcactccta ttcttcgttt
     6181 aagctcggat ctatattaat ctccagttcc attgtaaatg tgtcagattg aaaaaaatag
     6241 ctgtttttcc tttttataag aaaaaaggca aaaaaaacag aaagtaggtc ctccaagaca
     6301 ctcttcctgt tcccaatata ataaatgaaa gtaagaaatt atattttcaa gaaaacaaac
     6361 agaaaattat cataacacaa attatttata attgaaagac tagttttttt tcaagaaaag
     6421 catacaatag attgtgttag taaatacaga acacactaca tttagtgtct taaggagtcc
     6481 tcatacatga agtcatattt tcagttttta gctcatcagc taaaaggctg gccgcaacaa
     6541 aattattatc ttttcttttt cagtcttggc tgccaggtca tgactcttgt caatagtccc
     6601 atcacttggc tgtccgttat tacatttatc ggtaccacgc ttggtgtatt gtgtatttta
     6661 tccatcaatg cggcaaaatc agtcaacggt attttaggta ttttgtctgc gatttgtttt
     6721 attatcgttg ggttttcagc aaagaactat ttaagtatcg gtgaacagat tgcttatatg
     6781 atcacattgg atatccccgt attgttaagt aaagaatgga atcataatat ggcttcaaaa
     6841 atccgaaaat tcactggaaa aagctggttt atcgcaatca tcagtacttt ccttgtctat
     6901 cttgtttcag gattcctgat tggtcgttat acagatgatc ctcggccgtg ggtagatgcc
     6961 attgcttttt ctattagctt gactgctggt atcatcagtt tcttgcgtta caacaaccaa
     7021 tattactggt ggttagcttc agggcttgct caaatggtcc tttggtatat ttcatttacg
     7081 catggatctg caagtttggc gatgttcgtg aacagctctg tttacttgct gaatgatgtc
     7141 cttgcattta ccgtttcacc ttggtataac caaaaagaac gtgaacgtat gcaaaaaatg
     7201 gaagaagaat actacaacgc acaacaacac gcataaaaaa ac
//
LOCUS       DACENJ010000131         7191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.135, whole genome shotgun sequence.
ACCESSION   DACENJ010000131 DACENJ010000000
VERSION     DACENJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.135"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(34..294)
                     /locus_tag="IW748_001946"
     CDS             complement(34..294)
                     /locus_tag="IW748_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1104854.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(321..1622)
                     /locus_tag="IW748_001947"
     CDS             complement(321..1622)
                     /locus_tag="IW748_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1104855.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(1914..2372)
                     /locus_tag="IW748_001948"
     CDS             complement(1914..2372)
                     /locus_tag="IW748_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1104856.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(2385..3710)
                     /locus_tag="IW748_001949"
     CDS             complement(2385..3710)
                     /locus_tag="IW748_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1104857.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGRDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(3727..5139)
                     /locus_tag="IW748_001950"
     CDS             complement(3727..5139)
                     /locus_tag="IW748_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1104858.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(5156..5920)
                     /locus_tag="IW748_001951"
     CDS             complement(5156..5920)
                     /locus_tag="IW748_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1104859.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(6024..6899)
                     /locus_tag="IW748_001952"
     CDS             complement(6024..6899)
                     /locus_tag="IW748_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1104860.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         2323 a         1503 c         1062 g         2303 t
ORIGIN      
        1 taaccacttc tgttacaacc acttttagaa catttaaagc ttcgatgtga caaaccatct
       61 atgctgctcg atcataaaat tgatagcaaa aattccacaa agtacatatg tcccccatga
      121 aaacaaaaca ttcatatgcg gtacaatgat ctttggaaga atcacgatca aaaatgatgc
      181 cagtgcccaa atccatcgat tgatttgaat atgatatgta gctagcatat tcaatataag
      241 gtgactagcc gttaataaaa agataaaaac aaaaaataaa atcagccaat acattgtttc
      301 ccaccttcta aaaaccaaac ttagacagtc tggacttcgt tttctccacg actttgttca
      361 tctgacagca tctttttatc ataagctttt acaaatggat aatagataat aaatgctatc
      421 aatgtgttga tcatgatcaa gataacaaca cgccagtcat ttcctgctga aagataaccg
      481 ccgattggag ctggtaatgt ccatgttggc aaaacagtaa atctattgac catttctaat
      541 ttgactgcaa aataactgat aatagtcaaa actaaaggaa ctaagttgaa cgggatgaac
      601 atgtaaggat tcaacatcat aggagctcca aaaattatgg gctcattgat attgaagata
      661 ccaggaataa tagaaaaacg accaacctct ttcaagaatt ttgatttaca gaagaatata
      721 aacaaaacag ataccacgat cgttgctcct gcaccaccaa ttgttaccgt ccattgataa
      781 aactgttcag gagcgatata gggtaattgg gtggcagctg taccatctgc caatgccttt
      841 gcattctcat ccaacatgat cagccacatt ggacgaacaa cagcccctac aatactcatg
      901 ccatggatac caaatgacca caacaaatgg atcaaaaaca ttggtagtaa aacaccaagc
      961 aaattattcc ctaaaatatt ggttaaaggc tcgaataacg ataataaggc agcgtttaca
     1021 tcaaagcccg cgacgcttcg aatcagccaa gtaattgtta aaatgactgc acctggaatc
     1081 aatgcctcaa aactacgggc aacagcaggt ggcacttgtt ctggcatttt gatagtaata
     1141 tttcgatttt taaagaaacg atatacttcc actgcaaaaa tcatagataa gatccccgca
     1201 aacatcccag atgctcccaa agaactcata ggtaagaccc agccaagagg tgcctcttca
     1261 ggagttaaaa cgtttacagg aacttgtaac ataaagaacg ttcctaaaga taatatacca
     1321 cctgtcacac tatctaatcc atatgattta gctagatccg aacccataac aaaagttgca
     1381 taaatagcca tcaatcccat cgtcatccta aagggtaaaa caatcgttgc cgaataaggg
     1441 gcaataaatt cattccatcc aggaaacggt aaattgataa agaccataaa aaatgtccca
     1501 atcaaaatca atgacaatgt tgaaacaacc ccattacgaa ttgctaataa atgacgctgt
     1561 gtaccaattt ttgccattgg tgggacaagt ttctcttcga cccatttcat cagcttttcc
     1621 actaaaacca ctccttttca ataaaatatg aaccattcat tgcatgacaa cgcaaagaat
     1681 gttcataact aattatataa ttaagaaatt ttatttcaat atattctctt aaataatcaa
     1741 atttattttt aagaatcctg aaaataagtt ttctaaatca taggagcaac aaaaaaattc
     1801 gataattaac aaaaatcaaa atgattaaat ctagtgattg aaacatatca tttcaaaaaa
     1861 attttgtaaa ttcacccata aatatcaccc tatttaactt ttttcatcgt acactaaaat
     1921 tcaaaacaac tatctttaat ctttacttct tggttttgtt ttaataaatt tccttcaaaa
     1981 atgcctggag aaactacaat gatcaccgta tctttttgtg cttgctccaa cttcttcaaa
     2041 tctataatag ctaacagtgt cccttctttt acctcctggt tactttcaac ccgtatctga
     2101 aacggacttc cttcgagttc aactgtatct aaacccatat ggatcagtat ttttctgcca
     2161 gattcacttt cgatcgttat ggcatgtcgg gtaggaaata tattatcgat tattccttta
     2221 actggcgcat ggatatatcc atcatgattc gttatagcgt atccaactcc tatcattttt
     2281 gaagaaaaga cctcgtcttt tactgctgtt aatggaataa tttgaccact agctacagct
     2341 tttagaatgt ttcttgtttt aaagaaatgc actttttatc acctctactt taatgctggc
     2401 caatattttt tgtttacttt gatcatttca tctagtattt tttttgcgac tctggcattt
     2461 ggtacggttt gattcaatgt aaatgcttgt aaagcttttt gataagaacc ttcaaagtaa
     2521 gcatctacta gtaacttctc tactgcaact tgtgcctcca tcaatccttt atgaaaatca
     2581 ctgattggtt ttcttagtgt aattggctcc acaccagttg caccaacata tgccggtatt
     2641 tcaacaactg catctgaccg tagattcgga atcgcccctt gattaggaac gattagcata
     2701 aagcgacgac gatcatcatt caacaaggaa atagctatat cgacgatata ttgtccatgc
     2761 tccccaaaat taaagttcaa tacttcttcc acaataccgc ggcgtatttt atcagccatt
     2821 tctttggtat ttttctctcg tccatccatg accatattag cacgtgtata ctctttgtca
     2881 gtatgctcaa cgaccatatc aggatataag taatactcta agtaattatt cgggatatta
     2941 cttggaaaat ttttcgtgat gactgacatc atttgaaagg ttttttgcca agttttatct
     3001 cctatattaa aatcggcgac ttgcatttct tttgttatta gtttttcaat aatctctggc
     3061 ataatttctc gttgcaattc tttatcataa atcgatgtat accagccaaa gtggtttaaa
     3121 ccataatacg taacgatcca attcttacga tcataaccgt aattcactgc aatcgtttct
     3181 tcaattgaaa tagtcatatc acaggcatta atcattttta cattcggata tttacgacga
     3241 acagcttcag aaacgatcga ctctggattt gtataattca aaatccatgc tttgggggca
     3301 tagtcttgta tatgatcgac aatttccaat aaacctttga ttgagcgcat cccataagca
     3361 aatcctccga gtccacacgt ttcttgtcct actaatccat gtttaagtgg aatcttttcg
     3421 tccttttctc gcatctccaa tcctccaaca cgaatttgtg agaaaacaaa atcgcgtcct
     3481 gtaaaagcaa gctttggatc atccgttgca cgaatgcgaa tatttgaaaa accttttctt
     3541 ttcagcataa atttaacaat caagtacata tccatatttc tttcttcatc aatatcatat
     3601 aaacgtattt cagataatgg caaccgtttt tcattgttca aaatggcttg aacgattcct
     3661 ggtgtatacg tactcccgcc tccagctata gtgataatct gttttttcat catcatcttt
     3721 tcctttctat tcttctaata atttattgaa ttctgccata acataagtca cttctaagcc
     3781 gatgataatt tgataatttt tttcagttgg tttaatgatc ccgcttatcc ctaattgctt
     3841 gattcgctgt tcctcgacca tggtgggatc cactacctct acccgtaggc gtgtattaca
     3901 actatagaca tccaggatat tggcttttcc tccaaacgct tgtagcattt ttgttgccat
     3961 atattcataa tttttacttg ctaaccctaa ctctgtttct tctaaggtta cttcattgcc
     4021 aaactctgcc ttagcttcac gtcctaatgt ctgaatgttt ctcttattga tcaaataata
     4081 aaaagtaacg taatacaagg caaaaaagaa aattccgatt ggaagaatca aaatagcatt
     4141 ggtagcgatt cgaaaattga tgagataatc aacaatacat gctccgaatg taaaaccaat
     4201 tcgtatattg aataagtaac agacaatccc tgctaaaccg gcaaaaagtg catggatcac
     4261 gtacaacata ggagaaagaa acatgaaact aaattcaaca ggctcagtga tatttgcaat
     4321 aaatgaagtt cctgcaccgc ttcccatgat tcctttgacc atctctttat tttctttata
     4381 agctgcttta tagatagcta gacatatagc tggtatacca aacatcatta ctacaaaaaa
     4441 aactgataaa aacagtccag cctgaggatc tccgttgata aagcgtgtca tctctcctct
     4501 gaaaacttcc ttgctaggag atgtatagct tcctaaatcg taataaatat acgtattcaa
     4561 cacatggtgc agtccaaagg ggatcagcaa acgattcaaa aacataaata tccccacacc
     4621 taatgctccc atatcaacga gcagttttgc aaaattatca atcccaattt ggatgagcgg
     4681 ccagataaag cccaaaaaaa cagctgcgat tgtttgtaga atcatcacca tagtcaatgg
     4741 gaatttttcg cccgcaaaat aagaaaaaac tactggtaat ttttgttctt taaagcgatt
     4801 atacgtccat gccgctacta aaccggaaat aattccacca aaaatcccca tattcacttc
     4861 tgggtccata atttctaagc cttttttcaa ggtagcaatc gctaaaaaac cagtaagtgc
     4921 aggtattcct ttgtccttat ttttgacaaa accgattgca actcctatag caaataagat
     4981 atccaagttt ccaaaaacgg catttccagc tgcaccaatt actgggatgt tcagcatatc
     5041 gtcagctgat aaacgcatga tcaatcccgc taatggcatc gctgcaactg gaagtagcat
     5101 cgctcttcca agcttctgca catttgattt aacattcatt tttattcctc ctctattaaa
     5161 tatcctcata tgttactaac tcaatcaaag gatcattttt cactttttga gcaaatgctg
     5221 aatgaattaa aaattcgact tggtgggtac gaggatacac aaaacttgaa ccatttaaca
     5281 attcctgatc caaatacgct gcgtgacaca tgacttcgat cgttttatct tctctcaata
     5341 tcatgagtaa acttgccaaa tattcttcca cactcgtatt gatttgtcgt tttttttcat
     5401 cagtttctcc tacgacatcg aaacaaggct caaaataatc gacatgacga acttcatctt
     5461 ttcgttcaaa gttgctccga accggcaaat catatttttt tgctaatgta acaactacct
     5521 cctgattttc atccaaagta tgagtatgat gatgactgtc taaatgagtt ggtatgatcc
     5581 ccgcatgata aattttttga atctgagcat tccattcctg atacagttga tctttgtcaa
     5641 tgttaaatgg atgctgataa taagataagg atctgaactc tccgctttcg ataagagtat
     5701 cgacagtttt gaggagtggt ttgccacacg tcaatgttaa atggactcct acacctagcc
     5761 taggcatttc ctttcttagt ttcacagcat gctcgaaacc cggcatattt gccatcaatg
     5821 tagttgatgt caaaattcct cgctgataag catccataat cccataattc acaccatggc
     5881 tatagccaaa atcatccgaa ttaaaaatca cttttcccat ttcattctcc ttcttttttc
     5941 tactaaaata ggcgcaacca aataatcata gtccatttac tatgtgtcat ttggttgcgc
     6001 ctatagatag ttacgccctt attttaatag attcttgcta gatgaattgc caaatagatg
     6061 atttcatttt cttgaatggc aagttgaaat tgctgttcga tgatatcttt aatctttagt
     6121 gctgtacaat acgctttgct atgtttaaat gtttcagtaa aaaaaccaat attttctact
     6181 ggcgtttctt cctgataaat caagcgttta accaggaatt tgatatgaac cattaagcga
     6241 tgataagtca gtgattcttt atcataaacg tttgaatagg atttttctag atgggacaaa
     6301 atcgtggtcg tcatttctac tatttttaag ctctttaaac tattaggctc tcctagctca
     6361 gcattaatga tatgcatagt gatgaatcct acttcatttg accctaactc ggtttgatat
     6421 cgatgattga ttcgctcgac cacttctact gctatatcat attcatttgg ataaaggaat
     6481 ctcatttctg gcaacataat tgctgtgaca tctctatctt ctctttttcg ttgaatagca
     6541 aaataaatat gatctgtcaa tgtaatatag atatttgaat ctaatggaga ggagatccta
     6601 gctgttatca gttcaatcac ttcttccgca aaatccataa attcgattgg aatctgcgag
     6661 actatctcac tgaatcgttt gatcttttct ttagaatcta atacaaataa tttgtcagca
     6721 tcctcaacat caaatggctt actcacttta gcagaaaagc caatcccttt ccccatcgca
     6781 attttttcta ttccatcgtc atcgatcaat acagcattat gattcagctt tttcatcaca
     6841 tacaccgtta attcacctcc tctatatgat aaaacaaaac aacttgcaga ctccttcata
     6901 catatttttg tctccaaaca atttttaaag acaaaaaacg acctaagaaa gaaccctttc
     6961 ttaggtcgtg cctaattgaa tagtagcacc ctacataatt tacaaaaata gtttaacaca
     7021 taataaaagc gttatcaata tcttttttac tcgttttatg ataatacttg tatcgaattg
     7081 atttggaggt aattacataa aaacaaaaaa aatatagcaa ctctctataa gaaagttgct
     7141 atattgataa ttataccggc ggccggggtc gaaccggcac gtcctcaacg g
//
LOCUS       DACENJ010000132         7053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.136, whole genome shotgun sequence.
ACCESSION   DACENJ010000132 DACENJ010000000
VERSION     DACENJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.136"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            297..1850
                     /locus_tag="IW748_001953"
     CDS             297..1850
                     /locus_tag="IW748_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1104861.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(2231..4015)
                     /locus_tag="IW748_001954"
     CDS             complement(2231..4015)
                     /locus_tag="IW748_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104862.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(4030..5751)
                     /locus_tag="IW748_001955"
     CDS             complement(4030..5751)
                     /locus_tag="IW748_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104863.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            5851..6612
                     /locus_tag="IW748_001956"
     CDS             5851..6612
                     /locus_tag="IW748_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1104864.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         2240 a         1242 c         1360 g         2211 t
ORIGIN      
        1 cagaatgaac ttcattctgt ctcagatttt ttgttatttc aaattgatta attgattgat
       61 caaaaaggtt ttgattttca tgttctcaaa cacgttcaat gtctttttct tctgaatggt
      121 cttgtaagag atctctgtaa tcatcaaatt tttgtctatt aatcttagta ttttttgatg
      181 tataaactac ctgaaattga gagtacatac caaattcata aattttttat tagtgggact
      241 agtgattgta tggattttga cgcataatga atgaagtatg agaaaaggag agacaaatgg
      301 atcaagaact atttattatc gaagtattat cagctaaaca aaaaattatc gagaaagcga
      361 aaaaaagagc gaaaagaata atgttcaagc ttttaagctt agtatttttc tctggtttgc
      421 tttttttcgg agctgtatcg ttcctatatc gccatatgag tgcagtagtc cgttcagctg
      481 aagggatgca aaaattaaat aatttgtatc acttttcgtg gggagttggc ggaataacaa
      541 ttgttgtgct tattgttttt cttctactta tttggctccg ttaccatcaa ttgtcgcgtc
      601 atttcgatca atggcttagt caaagagcac aacagttttt acataataaa ccaacttctc
      661 aagatgatga ctatctttat tttgagaaga cttcgacgag tcaagctttt cggatacaaa
      721 aaagtgattg ccagttattg ctgagatcac ttgatgattt acctatctac ttaggcgaaa
      781 gcaaatcaaa attcggaatg aacacattcc atattttttt actagaaaac gaacccagat
      841 gggaaaaaag ccagtcttcg cctggtggat acctgacaac taagatgagt acattagtca
      901 ctggtttttt cctcattctt ttagctggaa caatctatta tgggagatac actcagccaa
      961 aaatgtcttc tcatataaat gctgcttctc acatgacaat gaactcaact aacgatacgc
     1021 atatacaaaa gacaaatgaa gggttgttag ctgatcctaa gcaaaaggta aatttgacaa
     1081 cacaaaagat aaatgatctc aaacttgata cagagacgca ctctctctat atgacaacag
     1141 atagtggtgc taattgggta tttgtgccga ttgattcgca atggttgcgt tttggcgaat
     1201 acaccctgac aacggggctg attcctgaag gttattggat ggacaacaca tatgatattt
     1261 ccgccgattt ctcatggttt atctattctt ctggggatga tgtacgtctg ctgacttcaa
     1321 gagataatgg taaaacatgg caaacgtcta ttatcaccca atcagttcag ccgatacgtt
     1381 atcgaaaagc ggtattttat ggcaacgatg gtctatttat ctgtacatca gccagtggga
     1441 tgtctgcaga aactttgtac atttacacta ctatggatgg cggaatcact tggataaaaa
     1501 gtggaagtac aatgatcaat cagccagtac aaaatgtcag cttcgttaca acttctttag
     1561 gttttgtttc gacacgtgaa agtttgtttt atacaaacaa cggaggacgc tcattcaaag
     1621 aatcagttct gtcgattccg gaaaactatc agacaggggg gatagatctt tttcaatcac
     1681 caaatgaagt tactcaaata agcgcgaata cgttagagac aaaattttat ttgttgaaaa
     1741 aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt ccgttcaact gataacgggg
     1801 aaacatggca attcgttcag caactatcgc aagtagaaca aggagattaa agaagagaga
     1861 ttgtgagcct gacgttcagc accgagtcac aagaaacaaa ttcaaaaaac agcttctcat
     1921 atttgttgaa tagtgtaact cgtgattcgg agttgacaag agcaacaaga agcgattttg
     1981 aagtatagct tctcgtattt tacaagatcg tgtcttattg ccgataggtt gtctcttgaa
     2041 caccgtttat tcgtaaaaag ggattgtgac aagtcttttg tcactactta ccgaacgaaa
     2101 ctgaaggagc tatccaaaaa gtatcattag tattattctt tgtgtttcta gtggcaccac
     2161 tagtacattt aaaaaagcta tcgatcatgg actaatcata catgatcgat agctttttag
     2221 ataatgtttg ttacgctatc tgaaattgac tattataaag actggcataa agcgttggtg
     2281 attggagtag ttcagaatgt gttccttttt cgacgatatc gccattcttc attacaagga
     2341 tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat cacaaaactt gtcctatctt
     2401 ttgtcacagc ttccattgct tgctgaatca atgcttctgt tcgtgtatct acgctagaag
     2461 ttgcttcatc taaaataacg acaggaggat tcgctagaat gactcgagca attgtcaata
     2521 attgttgctg tccttgagat aacgtgccgt tttcgctaga aatgatcgta tcatatccgt
     2581 ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc agccgcttcg ataatttgtt
     2641 ctcttgtggc atccattttc ccgtaggcga tattatcagc taccgttcct tcaaacagcc
     2701 aggtattttg taaaaccatc ccaaaagaac gacgtagttc tccacgtgaa agcttggtaa
     2761 tatcaagacc gtcgaattcg atctttcctt tattcagttc ataaaaacgc atcagcagat
     2821 tgactaaagt cgtttttcct gcaccagttg gaccaacgat cgctaccgtt tgttttggct
     2881 ttactgaaaa atcgacttga ttcatcaata ttttatctga tgtgtaacca aactgtacat
     2941 ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct tgcatctgga aggtctggaa
     3001 tttcatctgt ttcatctaat atctcaaata tcctatcgat tgctgccatt gcggattgga
     3061 tcgagttgat cacataagaa gcggtagaaa ttggttcaga aatctgattg atgtactgaa
     3121 gataagcttg taagaagccg atcgtcacac cacctgatag aacaagcata gcaccgaata
     3181 ccgcacttat gatgaaggcg agttgattaa tgaaacgaat ggccggatag atagcacagt
     3241 tcataaattg agcacgttta aaagcgttgt attgcttttt atttacttcg tgtatagcag
     3301 cttctgcatg agcttgctga ttgaatgttt tgaccactag attaccagct aagatttctt
     3361 ccattcgatt atttaaaaca cccagttcat actgattctg ttcagaaatc tgtttgtttt
     3421 tgttcgcaat cttagttgta acaattgaac tgccaacgat cagcaataag acgagcaaag
     3481 taagcttgct ttcgatataa aacagcatca gaccagcagc agctattgtt acgacagaag
     3541 taaaaaattg attgatacct gtcaataaca cttgagaaag ttgattgaga ccggtagttg
     3601 tccgactaat gatgtcacct acttgatgat tgtcaaaaaa cgacatcggt aatgttttga
     3661 atttttttgt gatttctttt cgcaccctca atgtgatctt ttcgcttaac gctgccattg
     3721 tccgttcctg aataaatgaa gtgatgctgc ttaagactga acagccgatc agcaataaga
     3781 cagggaacaa caatgcttgt ttgatatcac tgaaagaaag ctgtccaata cctgtttttc
     3841 ttatttctga taagagatca tcgatcccga ttcccatgat caagggcatg gctacgacta
     3901 ataggttccc gaataaactg catagaaaca atccgtaaaa atatttatgt tcaggtttga
     3961 tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt ttttttcttt tccatcgtat
     4021 cccttctttc tatattcctt gtgattttgc aaactcctga taagtttggc aattagacag
     4081 tagttcttca tgagtacctt gtccaacgat ttttccttca tctaaaacaa tgatgttatc
     4141 tgcttgttgg attgtactga ggcgttgagc aacaatcacg agagttttct ccgacatgtg
     4201 tttatgaagg gctgctctta atgctgcatc tgttttatag tctaaagcgg agaaactatc
     4261 atcgaataca tagatctctg ctggtttgat caacgctcga gcaatacata tccgctgttt
     4321 ctggccccct gaataattag aaccgccttg agcaacaaag ctgtccaatc cgtcaggaag
     4381 ggtgtccact agttcttcta attgagcgat cttgatcgct ttagacaact cttctgttga
     4441 ggcatattca tttcccatct gcaagttgct taaaattgtc ccgctgaata agaaagcctt
     4501 ctgagggaca taactgatgt gggagcggat ctgttcttga gtcatttgag taagaggaat
     4561 accaccaagg gttacttttc ctatcgtagg gtcactaagt ctcaagagaa gtttcgccaa
     4621 actacttttt ccagatccgg tcccgccaac gatcgctgtt gtttttcctt taggtattgt
     4681 gaaatcaata tcttctagaa ctggatcaga gatttcagta tatttaaatg tgacatgttc
     4741 aaaagcagct gcatttgtct gatcaaaggt tggttcagct actgttttga tcgtctcttc
     4801 ctcaatttcg atctctgctt gcagtatttc ttctatccgt cgtaatgagg caaacgattt
     4861 agggataatg accaatacca tagctgcgat catcaagtaa gcaagtgtca gcatagagta
     4921 ttcgactaca gcagtcacga caccgatttg taatgctcct tctgctacaa gatttccgcc
     4981 aaaccacaaa atagaagcat aaacgattcc catgagcaaa aaagcaattg gagtgaccca
     5041 agcaaatatt cggttcactt tgatcatttg attagcatag tcagcaaagc tcttattcgt
     5101 tcgcttttct tcatatgatt gattatcaaa ggcacggatc atattaatcc cagtaaagaa
     5161 ttctctcaat gtgatggtga tccgatccat ttttggctgg atactcaaag ataaaggtgc
     5221 ccctcgtttc atcagtcgat aaacaatcca tacataaaaa acaacagaga tcaatgtaac
     5281 ccatgttagt aatagagaat aacgaaacat catgagaatg gtgaaaacgc aaataaccgg
     5341 tgctggtaag atcatttggc aaaataaaac aatcatttgc tggacattat cgacatcatt
     5401 cgtcattctt gtcaaaagag atccagaact aaaacgactg atatccttca ttgacagctg
     5461 ttggaattta cggaaatatt tctcccgcgt ttggaatcca aacctagcgg aagccaatgc
     5521 tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga aacgctacaa gcagcatttc
     5581 aactccaatt ttttttataa ctgaggcttc gccacttgct atacctgtgt cgatcagctt
     5641 tgctactaat tttggtacac ctaatgtacc ggcgatttct aaacagatca tcacaaaggt
     5701 aaggagagcg agtcctttgt ttttctgtaa gaatgttttt aagatagaca tattattcct
     5761 ccttttttcc tgatactttt cgattctata ccttcaagta acttgaaggt caatagaaaa
     5821 aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa tgtatacgat tggccaagta
     5881 tcggaactat ttgatttgcc aaaatcaacg atccgctatt gggatgaaca aggattaatc
     5941 cgttcaagta gacaagaaga gaatgattat cgattgtttg atatcgatga tatttttatg
     6001 ctttatgata tcgtttttta tagaaaatta gatataccga tcaaacagat gaagaattta
     6061 tatggaaaaa cattgacgga gttatatgag acacttgatg aaacagaaag aagaatccat
     6121 aaagaactgg tggtgatgaa acaaaaacaa aaagagataa gggagcaaaa aaaacagttg
     6181 aaattgatga tcgatacaaa cgaggaagaa tttcctgtag aagagatccc ttttgactgt
     6241 atgatctcaa cggagtttga agatattgtg gaaatcaaaa aatttctacc taactattca
     6301 agctttggga tgatgagtat gccagcttcc agttcacaga cgatgtatgg attttttatc
     6361 gaccctagtg aagtacattt atttactcaa gacgtgattt gggagaaaaa agatacggct
     6421 gtttatcgtc gattcttatt gaaatccgag atgaaccatg cggaacgtaa taatatttta
     6481 gaaatcagag aaagaatgta tgaaaaagga tggaaaacag gagaagtgat cggccagtat
     6541 ctattgacga atacggatga aaacaatatc agaactgaat attatcatgc atggatagaa
     6601 atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa tgatggttgt tttagttata
     6661 aagaaaaatt ttctggttcg attacgggtt taatgtgggg gattattagt cgtggataga
     6721 tagaatttta gttttttaga ggaaatatgt ctttaaaaat ggaatggttg caattcttag
     6781 cgatatatac gatgtaattg tcctcttaaa aaagtatgga aaaataattg tattgggtgt
     6841 tatgtataat tcatggtgaa ctgagactaa tgagaagaaa aattcatatt gttatgagtg
     6901 agattgtatc agatgagaat gataaattaa ataggtgcat aaaagcatta cacttaaaat
     6961 acattgttgc ttacattatt acaatatgtt aataaggcaa aaataaatag atggattgaa
     7021 taaacatatt ttttaatcaa atagtcccta ctg
//
LOCUS       DACENJ010000133         7043 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.137, whole genome shotgun sequence.
ACCESSION   DACENJ010000133 DACENJ010000000
VERSION     DACENJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.137"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            187..516
                     /locus_tag="IW748_001957"
     CDS             187..516
                     /locus_tag="IW748_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1104865.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(803..1288)
                     /gene="vanZ"
                     /locus_tag="IW748_001958"
     CDS             complement(803..1288)
                     /gene="vanZ"
                     /locus_tag="IW748_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1104866.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1441..2352)
                     /locus_tag="IW748_001959"
     CDS             complement(1441..2352)
                     /locus_tag="IW748_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1104867.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(2780..3388)
                     /locus_tag="IW748_001960"
     CDS             complement(2780..3388)
                     /locus_tag="IW748_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1104868.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(3394..4425)
                     /gene="vanA"
                     /locus_tag="IW748_001961"
     CDS             complement(3394..4425)
                     /gene="vanA"
                     /locus_tag="IW748_001961"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1104869.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(4418..5386)
                     /locus_tag="IW748_001962"
     CDS             complement(4418..5386)
                     /locus_tag="IW748_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1104870.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            5718..7010
                     /locus_tag="IW748_001963"
     CDS             5718..7010
                     /locus_tag="IW748_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1104871.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT         2117 a         1490 c         1231 g         2205 t
ORIGIN      
        1 tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa aacgcacttg
       61 caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa ttaaggtaga
      121 ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga atgtgaggtg
      181 agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt aaatggaata
      241 aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa atgtttcagc
      301 gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat gtgcgtctgt
      361 gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt acgtttttta
      421 aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta ctctttagcg
      481 aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat ctatctatga
      541 aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag gaaatttatt
      601 aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt actatagtgt
      661 taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac gggcgagttt
      721 ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg tatgaaagat
      781 cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta cggtaaacga
      841 gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat tttttttgat
      901 tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca acagtatttg
      961 taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt atttcaaaag
     1021 taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct ttaaaattga
     1081 cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc tctctgaaat
     1141 tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta aatactgata
     1201 aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa tataaagcta
     1261 gcaatcctct agataatatt tttcccaata taatttcctc taattctttt gtttaagttg
     1321 tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag ctaacgttac
     1381 cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat tccgccatcc
     1441 ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta caccgtctat
     1501 attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg tatttttagt
     1561 aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa tggttttttc
     1621 ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca taatcgcact
     1681 atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg ttaactctgt
     1741 tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt ctatccactt
     1801 tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg ataaacctga
     1861 attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt ggtaaagcac
     1921 actttgctca tcaaagtctc gatagccact attaataata aaatgactaa cgccaccctt
     1981 tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca tataaatatt
     2041 actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat tcacgatatc
     2101 tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca gatttccttg
     2161 ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc cactattttc
     2221 taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct gagaaaacag
     2281 tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca ataaaaaaaa
     2341 caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga aatatttcta
     2401 gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc cccgtaaatt
     2461 acagtcaatt atttcgccct actggattta ctattatcac caatgtagat gaattttcat
     2521 aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg ggcggtgtga
     2581 aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca gcgcacgcag
     2641 ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat atataaattc
     2701 cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta tatagtttgt
     2761 ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg gggtatggtt
     2821 cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca ctgttttcca
     2881 tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca tttgccgcat
     2941 gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc tcacccgtgt
     3001 ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat tttgaagcca
     3061 cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt tcctttgtca
     3121 ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga ttaggacggt
     3181 aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt gccttcaaaa
     3241 gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg tcaaccggtt
     3301 ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg tgtactattt
     3361 catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta atacgatcaa
     3421 gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg gataacgact
     3481 gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc cgttatcttg
     3541 taaaaacata tccacacggg ctagacctct acagccgagc gctttatata ttttttttgc
     3601 cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg ttataactgc
     3661 gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc cgtactgcag
     3721 cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata ccgcacaacc
     3781 gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt gtcttgccga
     3841 ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga aggatgagcc
     3901 tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg gcctatcatc
     3961 tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa cgatgtatgt
     4021 caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa aagggatacc
     4081 ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat gcaaagctga
     4141 aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa gtaatccgtg
     4201 cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc attccgcgca
     4261 aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata acggctcgta
     4321 tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata cgtcatgctc
     4381 ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc tgtctccttt
     4441 caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct tgctcggtat
     4501 aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta agtaaaaatt
     4561 gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct ccttccaata
     4621 catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac tcataggtat
     4681 ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt ctctgtattt
     4741 gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc gtaacgatat
     4801 cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt cggctgcgac
     4861 tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct ttgcctatct
     4921 ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg tcgctgtcca
     4981 acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt actgccataa
     5041 gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca ttgtcgacag
     5101 tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg cttcgggtag
     5161 aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa atctctgatt
     5221 tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat tccgacacgt
     5281 tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg aatgcatctg
     5341 cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt aagaccaacc
     5401 ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt caaatctttc
     5461 tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa gaacgagccg
     5521 tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt gagattttcc
     5581 taaaaaatta tggctctata ctttttgatg ttggtggatg acaccagcat cttttttatt
     5641 ttccaaaaca tatagaaaag acaccttgaa atcctttaga attaatttgg cgaaccaacc
     5701 caaaaggagg aatcaagatg tcttacactc accttatcaa agaaacatta gatattctag
     5761 acttatcagt tacttttaac gaaaattgtc tcacaaaaga aaaatacaaa gggcaaatct
     5821 gtcatattta tcgtggaaac ttgatttata cagcccaaga atgcatccac tgtaaacacc
     5881 agattgcttc agatattgtg cgttggggta caacaaccgt ccggttgtta atgaacgatg
     5941 tttctgaata ccgtacctac ctcgaattga aaaagcaacg cttcaaatgc aaagcttgtc
     6001 agcggacctt tgtcgcagat acttctgttg cgaaaaaaca ttgtttcatc agtgagaaag
     6061 tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc aatgacagag atcgccgctc
     6121 agaaaaatct atcggtctct tctgtttacc gtattatgaa gcgattttat cgtccgttga
     6181 atccttttcg gacaccgtta cctaaggtac tttgttttga tgaattcaaa tcagttcggg
     6241 gtgtttcagg tgctatgagt ttcatcatga tggatggaca aacacagcgc ttattggaca
     6301 ttgtcgagaa ccgacaactg ccttttttaa aacgttattt tagtcatttt tcacgagaaa
     6361 ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc tccttatgta tcgctcgtta
     6421 aaaagctgtt tccaaaagca cagctgatta ttgaccggtt tcacatcgtc caacatattg
     6481 gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca actattaaaa agcaaagaac
     6541 aaaaacacta tcaattaggt aagcaattaa aaagatactg gaaacttctt caaaaagatg
     6601 aacgcaagct tgattatact cgacgccttt ggcgacctgg cttcaaagct catttaacag
     6661 agaccgatat tgtggatcgc ttactgaaag ggagccctgc tttgcgtgta ggctatcagt
     6721 tatatcaaga ctttctttac gctgtaaagg aacgagacta cgtatcgttt gaagaactac
     6781 ttaccaataa tatcatgctt cccgaaggct atcagacgac cttgagaacg ttccagaaat
     6841 ttctgccaca aatcaaaaat gctttacaac aaagttattc aaatggccca cttgaatgtc
     6901 tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg ctttcgtagc ttttataatt
     6961 tcaaattgcg catcatgatt cgtcatggca acgcattgat tttcaactaa aaaagatcaa
     7021 gaggaactga tccacttgat ctc
//
LOCUS       DACENJ010000134         7017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.138, whole genome shotgun sequence.
ACCESSION   DACENJ010000134 DACENJ010000000
VERSION     DACENJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.138"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(35..1381)
                     /gene="gor"
                     /locus_tag="IW748_001964"
     CDS             complement(35..1381)
                     /gene="gor"
                     /locus_tag="IW748_001964"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1104872.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1515..2756)
                     /locus_tag="IW748_001965"
     CDS             complement(1515..2756)
                     /locus_tag="IW748_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1104873.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(2774..3697)
                     /locus_tag="IW748_001966"
     CDS             complement(2774..3697)
                     /locus_tag="IW748_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1104874.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(3918..6410)
                     /locus_tag="IW748_001967"
     CDS             complement(3918..6410)
                     /locus_tag="IW748_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1104875.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6417..6884)
                     /locus_tag="IW748_001968"
     CDS             complement(6417..6884)
                     /locus_tag="IW748_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1104876.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         1913 a         1546 c         1138 g         2420 t
ORIGIN      
        1 tcaataaaaa gctgttggaa ctgttaatat taaattatcg catcgttacg aattcttccg
       61 ctcctgttgg atggatggct actgtattgt caaagtcttc tttagttgca cccatcttga
      121 tagccacggc aaaaccttgc agcatctcat cgacaccaac accaatagca tgtaagccga
      181 cgattctttc ttcttttccg acacagatca atttcatttc gcatttttgg cgataatcat
      241 ttaatgcaaa gtacattggg gtaaagcgtg aacgataaac tttcaattca ttttcgccat
      301 atttttccaa tgcttcttct tctgtcaaac caattgttgc aattggtgga tgcgtaaaga
      361 cgactgtcgg aacaagttta tagtcaagat aagaatcttt ctttccatta aataaacgtt
      421 ctgataaacg tctaccagct gcgattgcaa caggagtcag atctaattta ccgatcacat
      481 caccaacagc aaaaatatgt tcatccgttg tattttggta tttatctact ttgataaacc
      541 cttttttgtc caattccaca tttgtatttt ccagacctaa tttttcggta ttcggtacac
      601 gtccaccggc aaaaagtaca caatcaccag ttaaggtttc accattttca aatagcaccg
      661 tatattcgtc attgttttta gtaatttctt tagcaatatg atgtgcgtaa gtttttattc
      721 cagcttcttg gtacatttgg atcaaattgt ccgaaagcat tttatcaaac gttctaagtg
      781 gtcgttcttt tcgatatgcc cacatgactt cactacctaa accacgaaat acacctgaaa
      841 gttcagccgc aatgtatcca gcacctaaaa cgattgctct cttaggtaac tctgttaatt
      901 caaaaaatcc atttgaatca attgcgtatt ctccaccagg aatggtcatt ttacttgcac
      961 ggcctccagt agcgatcaag attttaggag cacgataggt ttcaccgttt acttcgatcg
     1021 tttgactatc gataaaagta gcgtaacctt ttatagcctc gacatgattg ctatctaatc
     1081 ctctttgata agcaccgtgt agaaaatcaa tatatttttc tcgtctttct actagaccgg
     1141 cgaaatcaac attttttaaa tctgcttgga taccgtaact tgaggcatct cgttcaattg
     1201 tttccaaaat cgtacttgct tgccacataa cttttttagg tacacaaccg acgttgacac
     1261 atgttccgcc caattcattc gcttcaatca gcaaaacttt tgcgccatgc atccctgctc
     1321 tgtttgctga agcaataccg ccagagccgc caccaattac gatgtaatca tatatttgca
     1381 ttttgttcct ccttattgaa gagatcaatc aaaaccttta tttaacgatc ctttttctac
     1441 agaaaacaag tgtaacactt ttcttgaatt ttgtatgcga agatgcttaa aacagggata
     1501 tatcagcttt ttccttattt acgttttcgg atcatatctc caggtttcac cggctgtgcg
     1561 actggcatag ttaaaatcat cattggattt ggtgctcggt caatggattc gccttcttca
     1621 ttatgcataa cttcaacggt ttgactgaaa tgactgaatc ctggaccata aaattcgatt
     1681 tcatctccta cagaaaacaa gtttctctga cgtatcgtag caatctgtgt tgcctcatca
     1741 taagataata cttcaccgac aaatttatat actgggattt tacgacgttc accgaataat
     1801 tgttcgtttt cagttggtgt attgtagtaa aaacctgtag ctaattctcg ttgtgctact
     1861 ttccataact cgtcgatcca ttcttgcttg cagacatagt tttcaggatc ttccatataa
     1921 ctgtctacag ccgctttata gacatttgac accgtagata cataatggat cgatttcatt
     1981 cttccttcga tcttgaagct gtctacgccg ttttcaatca attctggaat atgttcgatc
     2041 attgacatat ccactgcact catcgagaat tcttcttcca cttcgcctgt atcagttaaa
     2101 ctgtttcgtt cttgaccaaa aggcatatcg aacaatccat acttccagcg acaagattga
     2161 gaacatccgc cacggtttgc atcccgcatc gacatatggt tggacaatgt gcatcgacca
     2221 gaataagaga tacacatggc accatggata aatgcttcga tttcggtatc cgtattttta
     2281 cgaatctctg ccacttcctc cattgaaact tcacgagcta aaacgacacg ttccaaccct
     2341 tcttctttcc agaattccaa tgtctcaaaa ttagttgctg aagcttgagt ggataaatgg
     2401 atcggcagac ctggggcttc tgtcgcacaa atttcgatca aagctggatc agaaacaatc
     2461 actgcagaaa taccgacatc acgcaaactg cggaaaaatt cccctgctcc atcttcattt
     2521 ccttcatggg tgaccatatt agcagcaacg tataccttcg catcatgttc ttttgcaaaa
     2581 gcaactcctt cagccatttc ttcataagta aaattacctg cacggcttct taaaccatat
     2641 gcattcccgc caatatatac tgcatctgct ccataatgga tcgcggtttt taacttttcc
     2701 agtgttcctg ctggcgctag gatttctggt cgtttcaatt ttctttttac tgtcatttct
     2761 ttcatcatac tccttatttg atagcagaag gatcaatata aaagaatcct gtatctaatt
     2821 cacggttttc aggatgcaat ttactgattt cgctagtcat tttttccgct ttttcttgat
     2881 cccagctacc ttctaaaatt tgtttttttg cttcggcgaa tagtcgagtg atgtttacga
     2941 atttttcgcc tggagtaaat aatccgtcca atttccatgt acagtaatga tgctgataaa
     3001 gttcattcaa ttcattcgag agattcagat cattgctggc aaaaatatgt gtgccatgac
     3061 tatcttcata aatagaataa tgtgtctctt cttttttagg ttccgagata aaaagacctc
     3121 tttcacgatc tttttgttcg tcttgtttcg tatagttata gtaattctgt agcagtggac
     3181 gtttggattg atggatgcat gtagcaccat acacaagggt ttctacagga atatcgagtt
     3241 tttcttccat cgccttcatc tcttcaaaag gaacttcacg agcaagtact gctccaactg
     3301 cgcctttttt cgcccagaaa ttgatttgac gtgcgctcgt tactaatgtt tcagcatcat
     3361 aaatatacgg tatacgcaat tcaggatttt ttttcatcgt ataaataata cctgtatcac
     3421 caactgtgat cacatctacg tttatttctt ttaaaaaagc caaatattca ggtacatttt
     3481 tcattttttc aggatgaatg atcccattta cagcaatatt cgctttttta ccatacttgt
     3541 gtgctaattc taccaattgt cgctgttctt ctcgtgtaaa tgaagcaggc agacgcaatc
     3601 caaattgctc ttcaccgaaa tatagtgtat ctgcacctgc agcaagcaac tgttctgctt
     3661 gttcaatagt ttctactgtg gttatgagtt cgatcataat tttctcctcc tacaacttca
     3721 taatagtagc atagctctaa aaactttcca atacttcttc tatttaaagt cggatttttg
     3781 aaaattattt cacaaaaaat caaaataaag ttcgatatat tctgtccaaa tataaaaaga
     3841 gattgtgaca aaaatcttgt cacaacctct taatcaagtc ttactattat aaaaattcag
     3901 ctacacaggc caagtaacta tagggaacct actgtttcct ttttaggtcc gcgaacattc
     3961 aatgtgatct taccttttga agcacctact gttacgtggt cacctaaacg aatttctccg
     4021 ccaagcaaag cctcgctcaa tcgatcttca atttcttttt gaagtgcgcg acggattgga
     4081 cgtgcaccat attcaggatc aaatccagct ttcccgatca catcgattgc agatggtgtg
     4141 attttcactt gaatatcttg ttctttcaat cgtttgataa tcgctttgct catgatttgg
     4201 acgattgtat ggatttcatc tttactcaat gaatggaaga cgacagtctc atcgattcga
     4261 ttcaagaatt caggtcggaa agctttcttt aactcttcta agatacgttt ttgcatggct
     4321 ttgtgatctt tagtaacatc ttggacattg aatccgacat ttttttcttc tcgaatttgt
     4381 gtcgctccga tatttgaagt cataatcatg attgtatttc taaagtccac ttttctacct
     4441 ttagaatctg ttaaatgacc atcatccaat acttgcagaa gtaaattgaa aacgtctgga
     4501 tgtgctttct ccacttcatc taagagaatc actgaatatg gtttttgacg aattttttct
     4561 gtcaactgtc caccttcatc ataccctacg tatcctggag gagacccgat taatcggctt
     4621 gtactgtatt tttccataaa ttctgacata tcaacacgga tcagggcatc ttcacttcca
     4681 aacatgactt ctgaaagtgc tttggccaat tctgtttttc cgacaccagt tggtccaagg
     4741 aacatgaatg aaccaattgg acgatcagga tctttcaatc cgcttcgtgc acggcggatc
     4801 gcacgtgaaa ctgctttgac agcctcatct tgaccaacga cacgttcatg cagaagtcct
     4861 tccagttcta acaagcgttc gctttctttt ttctccaact gctgtagagg gacaccagtc
     4921 cactccgata caactgtagc aacatcttct tctgttacac gatcagaata accttgctcc
     4981 tgttttgctt caattgcaga aactttttgc agctttttac gtagtgcttt ttcttgtctg
     5041 cgtaattgag cagcattttc aaagtcttgt ttttggatcg ctgcttcctt ttcttgtact
     5101 agttcatcga tttctagttt gatcacagtt gattttgtta agtgatcggt ttgatccaaa
     5161 cggactttcg ctgctgattc atcgatcaaa tcaattgctt tatcgggtag ttgacgagaa
     5221 gtgatataac gaacagaaag attgactgct gctcgtacag cttcatctgt gatctccact
     5281 ccatggtgct cttcatatct tgtacgtaat ccttgaagaa tcacttctgc ttcttcagga
     5341 gttggctcat ctacttgaat gcgagcaaaa cgtcgctcta atgcagaatc tttttcgata
     5401 tatttttgat attcatctag ggtcgttgct ccgatcgttt gtaattctcc tcgagctaac
     5461 gccggtttca agatattcga agcatcaatt gctccttctg ctccacctgc accaattaag
     5521 gtatgcaatt catcaatgaa taagattact tgaccatcat tatagatttc gtcgatgacc
     5581 tttttcattc gatcttcaaa ttcaccgcga tatttcgtac cagcaaccaa agatcccata
     5641 tcaagcatca tcaggcgctt ttcttgcatg tcttctggca cttcacctaa aatgattttt
     5701 tgagctaacc cttcagcaat ggctgttttg cctacaccag gttcacccac cagtacaggg
     5761 ttattctttg tccgtcggct aagaatctgg ataagccgtt tcacttctct cgaacgacca
     5821 acaactgggt ccagcttgtt ttcttttgct aatttcgtca aatcacgtgc taatgaatct
     5881 agcgtaggtg ttccttgcgg tgtttgccgt ttatcttgta catttacatt tctacgacga
     5941 ttcattcctt ttccagcaga atcaccagct agccccattt tttgtttcag aacttgacgg
     6001 gcttttgaaa ggctgatacc taagttgatc atgattcgag tagccatgat actttcatct
     6061 tttaataagc tcatcaaaat atgttctgtt ccaacttttt gagaaccagt tttcttcact
     6121 tcgctgctag ctaaagcaaa aacttgtttc attcttggtg aatatggaag aaagactcct
     6181 ttagggtaag aacggatacc accataacca atcagatgtt caatttcttc ataaatttcc
     6241 gtttcattca aatccatttc acgtaatact tttccagcaa ttccattttg ctcgaccact
     6301 aaggctagta aaacatgttc tgaactaact gattgatgtt tcaaccgttt tgcttcttct
     6361 tgtgcaattt ctaatactgc ttgggctctt tcagtaaaaa gttcttccat ctaatctcac
     6421 ttcccatctt cataacttaa acgttccaga aatgctcgga gaatccgagc acgtgtatga
     6481 tcttcatatt cattgaaatt caatgtgttt tttgcaattg cactcaacat caagtttcct
     6541 tctttttttg taattatttg gtcttcataa agtttttgaa taatagcaaa agcatttttc
     6601 tcactgattg tatcatcaaa taactgattt atctgctcaa gtaattgttt tttatctgaa
     6661 atccttactt ttgcaattcg tatataacca ccaccaccac gtttgctttc tactagataa
     6721 ccacgttgga tggtaaaacg ggtattaatc acatagttga tttgtgaagg aacacagtta
     6781 aagaggtctg ccatttcaat ccttcgaatc tcgatcattt cactattttc cagaattttc
     6841 tttatatatg attcaattaa atccgaggta ttctgatgtg ccatcttatt cattcccttt
     6901 gactaatatt gaccttaatt ataacgaatt tgataataga aagaaagaag aacgcttttt
     6961 gttaaataaa tcctttagaa tcaacaaaaa aaaatacgga atatactatt ccgtatc
//
LOCUS       DACENJ010000135         6957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.139, whole genome shotgun sequence.
ACCESSION   DACENJ010000135 DACENJ010000000
VERSION     DACENJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.139"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            170..955
                     /locus_tag="IW748_001969"
     CDS             170..955
                     /locus_tag="IW748_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1104877.1"
                     /translation="MRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLGKLKYSSYI
                     KVVNYFVNTIFSEFSEKEKNEISEQIRKNMSYCSFSSADCKEKNEEYVVQLLTQIYFF
                     SRIFPYDTTHLTIRATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFW
                     MLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSK
                     YNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(1028..1675)
                     /locus_tag="IW748_001970"
     CDS             complement(1028..1675)
                     /locus_tag="IW748_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1104878.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1683..2369)
                     /locus_tag="IW748_001971"
     CDS             complement(1683..2369)
                     /locus_tag="IW748_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1104879.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2375..3448)
                     /locus_tag="IW748_001972"
     CDS             complement(2375..3448)
                     /locus_tag="IW748_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1104880.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3670..4344
                     /locus_tag="IW748_001973"
     CDS             3670..4344
                     /locus_tag="IW748_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1104881.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4349..5689
                     /locus_tag="IW748_001974"
     CDS             4349..5689
                     /locus_tag="IW748_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1104882.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(5838..6824)
                     /locus_tag="IW748_001975"
     CDS             complement(5838..6824)
                     /locus_tag="IW748_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1104883.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2144 a         1304 c         1209 g         2300 t
ORIGIN      
        1 tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc caatcgaaaa
       61 aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt ttcaggaaga
      121 aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca tgagagaatc
      181 ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga taaatatcga
      241 taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat
      301 aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa
      361 tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat ccgctgattg
      421 caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt ttttctcccg
      481 tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg cacagcagat
      541 gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat tttattccac
      601 tctactgaat cagaatctga tttattattt atctagctat atcttttgga tgcttatcgg
      661 taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca gcgacttaag
      721 tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg tgcataaaat
      781 tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc ttctcgagta
      841 tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata tattgattga
      901 tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt tatgaataat
      961 caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata cacatagctg
     1021 tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag tcttttaaca
     1081 actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt acgagggtca
     1141 tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa tgattgattc
     1201 ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct ctacgtttgg
     1261 ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag atactttcgt
     1321 gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt
     1381 tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt ttgatcgtat
     1441 acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc gacatcccca
     1501 tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa aatatctcca
     1561 gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa gtttcttttg
     1621 gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt gtcatgaagg
     1681 attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg ataaacgcga
     1741 tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt ttgtttctca
     1801 tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc caaactagct
     1861 gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc aattgccact
     1921 ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga ctggatatct
     1981 aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt aacttggtca
     2041 actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct ttggagaata
     2101 aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc agttagtgag
     2161 tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga aatggtaagg
     2221 aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc ttcctttaca
     2281 taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa tgttttggta
     2341 acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct gtaattgggt
     2401 ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca atgatcaaaa
     2461 tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa
     2521 tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc ccaacaataa
     2581 aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt gctttcatta
     2641 ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg accacggcag
     2701 caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg gcagaatagc
     2761 caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc gatgcttttt
     2821 ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt tcttcttcag
     2881 aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac gcatagatga
     2941 cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt
     3001 aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga ttttgagaga
     3061 tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt aataaaaatg
     3121 cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg attgctccgc
     3181 tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct cctcttgtta
     3241 attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct tgtgcgtccc
     3301 aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct gacaacatga
     3361 atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt aatttagaat
     3421 agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag attgatagtg
     3481 acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt
     3541 tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca tgagtcataa
     3601 aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc aaatcaatga
     3661 ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc caataatata
     3721 aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag tgaaggactg
     3781 ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat gccggaaatg
     3841 aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc agtattgatt
     3901 ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg tgcagatgat
     3961 tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa
     4021 cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag ttgtgattta
     4081 aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa agagtttgaa
     4141 ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca gatatttgac
     4201 cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta tatgagcaac
     4261 ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt acgtaatgtc
     4321 ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa agcaacgtag
     4381 tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt tgggattcat
     4441 tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat tagagaaaat
     4501 aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag gcgatccctt
     4561 tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat taatggaacc
     4621 atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg agatattgaa
     4681 taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa
     4741 tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg ccctctcttt
     4801 ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca tccaaatttt
     4861 agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta tgattttgtg
     4921 tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta gtctaagcat
     4981 gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg cttctcatga
     5041 attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta ccaagcctaa
     5101 tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct
     5161 cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc agcagctgct
     5221 ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac catttcaaga
     5281 gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat tagaagtacc
     5341 aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg cattaaaata
     5401 tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag aatggctgat
     5461 tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga tttttgatcg
     5521 attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc ttggcttggc
     5581 aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca attatcttcc
     5641 acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat gcttatccct
     5701 aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat aaacggtgtt
     5761 ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac ttctgtcaca
     5821 acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac atcaccgctt
     5881 gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac atcttcatgt
     5941 tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac atctacttgc
     6001 tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa
     6061 gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac cggtacttct
     6121 accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt tgtcacatca
     6181 tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc tagatttggt
     6241 ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc aggtacgtcc
     6301 atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa gcctgtcgtg
     6361 ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc cattgtgctt
     6421 gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg ctgacgatag
     6481 ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg atataaagga
     6541 tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt atcttttatt
     6601 ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt ttcaacatct
     6661 ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt ataaataacg
     6721 atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc
     6781 ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat tcctcctagc
     6841 aaatagattt atgattggta tattgttact tataaattta ttctatagga gacgattgtt
     6901 ttctacaaat atttagattg gaatttgatt aaaacaattt aattgaaatt tgaaaac
//
LOCUS       DACENJ010000136         6855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.140, whole genome shotgun sequence.
ACCESSION   DACENJ010000136 DACENJ010000000
VERSION     DACENJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.140"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            9..284
                     /locus_tag="IW748_001976"
     CDS             9..284
                     /locus_tag="IW748_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HAQ1104884.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            393..2219
                     /locus_tag="IW748_001977"
     CDS             393..2219
                     /locus_tag="IW748_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104885.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSIGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELVVSSEETEEVEEIETVSENEEETEEIFDFEDEEEVENVQKGDLS
                     TSSQNGLEAVNDPQVPPYISSQTPNYSPQYQAPNVLQPLRKHETVQLVTLKEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDIAWKKPYDLDVKKNKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            2232..2948
                     /locus_tag="IW748_001978"
     CDS             2232..2948
                     /locus_tag="IW748_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104886.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKG
                     HYENINAQISCPLDRNGETTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     TKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEIKSFDKRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            2961..5819
                     /locus_tag="IW748_001979"
     CDS             2961..5819
                     /locus_tag="IW748_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1104887.1"
                     /translation="MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFD
                     PKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVI
                     QSYSQLETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLD
                     TDSSVTSSAEQERVITVERASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMP
                     YRYQFLAEDFDTQRDINEIDIACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPI
                     SEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLSEQEMKINIYLKESQKE
                     AIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQIMIL
                     EQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI"
     gene            5870..>6855
                     /locus_tag="IW748_001980"
     CDS             5870..>6855
                     /locus_tag="IW748_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104888.1"
                     /translation="MQWLKYKWPIIVSLIIILFMAYPQRVFALPWITDNKEVLKFLTK
                     YQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFSFVESSSLGAVY
                     QSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALILLMPTMISSGIQ
                     FAKTFYKDSTTITKADGGVAWSLIEDGVTDLVYINKNDAYDRLGKDGVQKNALKEKNF
                     FSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDGWMSMFSDSFKG
                     GYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLVFATDI"
BASE COUNT         2606 a          879 c         1342 g         2028 t
ORIGIN      
        1 gaaataaaat gaaaaattta gttatcagca agtttgcaat gttaccaaga atggattttt
       61 tcgataatgt aagtaagttt atgtcaaata tgacttcaaa actacaagga atttcactat
      121 ctgtaattat cttttgtatt gtggttactg gtttgatgtt tcttttcgga gaagggccaa
      181 gtcgtactgc taaaaaatgg ttaacttata tcgtgattgg ggcaataatt atcttcggag
      241 cttcaactat cggacaaacg atcaagtccg ttggtggttt ctaaggggaa gcttcttaga
      301 actaaaaaat tcgcaagtat tgcatgaaag agcattgagt gattaatgct cttttttatt
      361 ttgaaaaact ataaactaag gagatttaaa ctatgaacca acgattcgaa tataattttg
      421 aagatgtaga acctaagtta ctgaaaaaag gaaaaggatt aaaaaagaaa aataaatata
      481 ctctcaaaca agcagaagca ttagtaggat ggattcaaca caattggtca gacaagacaa
      541 caattacctt gatctgttca gacacagaag aaggaacaaa gtatgctttt gatggaattg
      601 aaattactcc attttcgatt ggcggacaag aaaaattatt gtttcctgat attgaaggac
      661 aaattttaca agcgccggac agcatgattt ccgatcctaa taaactgatt ttggatatta
      721 acgatcaata caaacacgaa ccagacgtag cagaaccaac aaaagaatca aagtcaaaat
      781 taaatttgaa gctcccttca gtagtattac catttgctaa gaagaaaaat aaacttatga
      841 acgaattgat tgaaccagaa gaaacggata tttatgaaga agaattagaa gaacaagaag
      901 agatcgaaga attagttgtg tcttctgaag aaacagaaga agtagaagaa attgaaactg
      961 tttctgaaaa tgaagaagaa acagaagaaa tctttgactt tgaagatgaa gaagaagtgg
     1021 aaaatgtaca gaaaggcgat ctatctacaa gtagtcaaaa tgggttggaa gctgtaaatg
     1081 atccacaagt tccaccatat atatcttcac aaactcccaa ttactctcca cagtatcaag
     1141 cgcctaatgt cttacaacca ttaagaaaac acgaaacagt gcagttagtt acgcttaaag
     1201 agtactgtga tttgggagaa gaaattgaaa cttcaatttc tcaaatggtc gaaaagttga
     1261 aaccagaatc tttattcaaa ttcataggga ttccttctac aggtcttaca aatactcgta
     1321 ttgatgaata tagaagtaac catgctatgt tgcgattagc tgaagtaaaa tttgaaaatt
     1381 taagagagca ttattcacgg caaattattt cttttaaagc agaagcccta gatcgtttaa
     1441 gaactgcggt agatattgct tggaagaagc cttacgattt agatgtgaag aaaaacaagg
     1501 ccgaagagtt atcaaaaatg gaagcagaag cagaagctaa attaaatgag ttcgtgaaac
     1561 gtcaggaaaa caatgtaaat gataaaatca aaaagtttga gatcgaacaa gaaatagatt
     1621 taaaaaactt tattgcaaga caagaggcag ataaagcggt atttattgct acagagaaag
     1681 atcggactca aaatttaatt gattctaaag cggaatcaat caatgcagat ttacttgaaa
     1741 caaaagataa atttattgat gaagaaatgt acaaaatgaa aattgaaatg aacaaaaatt
     1801 tatttgatgg caagcaaaaa attaaatcag aattggctac tgaactcgct aaggcaaatg
     1861 ataatgtttg ggaaaatgcg ctgaaattta ttcaagaaat tcaagatgaa attgaagcta
     1921 aaacaccaat ttgggctgct gaaatcaagg agtttaatag tttagaagaa caagaacatg
     1981 aacgtatgaa gcaaactaaa gaacttcaat taaaagagga aagtattgaa atcaagcgtt
     2041 tggaagctga aatgaatttg aagcgtatgg aagaattaga tcatgaaaat aaaatgctta
     2101 aaattaaact tgaaactgcc ctttcaaaaa atgagttatt agctatggaa caagatacaa
     2161 agcaagtggt gaatggatca aacagagttg gtgcacttaa tttatttagt aaacgttaaa
     2221 ggagaacgga tatgagtgtt tttaataata aagttatgtt agggaactgt tatgtgaacc
     2281 acattggttt ttacgatgga ggaacataca gagttttatt cttacgttta tctgttttta
     2341 gttactcaaa agatggaaag gggcattatg aaaatattaa tgcacaaatt tcttgtcctt
     2401 tagatagaaa tggggaaacc acgactacaa gtagtcaccc taaagccttg atgaataaat
     2461 ttatctttga aaagttggat cgcaatttgg ttgaggggat gaagataagt attcatggaa
     2521 atattcgtgg aacagaacaa gtgcttctta acagtggttg gaaaaacttt ggtcaattat
     2581 cgcctttaga aaaagaagaa tatcaaaaaa ttcctaaaaa tgaattagta acaagaaatc
     2641 aaacatatat ctatatagaa gaggtaagta tgccagaaaa agaagaattt agaaagtctc
     2701 aatctaaggt aacaaaagaa gatgtggaag ttgcagaacc gaatcaagca gatgaaattt
     2761 tatccgatga tactgatcca gtgaatgatg aaggagaggt tattgaagtt tttgaagctg
     2821 aaattatcga agaggaagaa gaaattaaaa gttttgataa aagaatgtat cgagagaaac
     2881 caaaaggtta tgtgaatttt gaagatatgt attatgacga atattctaat gaaccaccga
     2941 tcaattagtg ggggtcaaat atgaagctag tacaaaacaa tgaagtgaaa gaaataaaaa
     3001 acggctttgt taaaaactcg aacaaaattt tgggaaagct gaaaaaagta ggtagtttca
     3061 aaaaaaataa atatcatact ggaaaaattg agggagaaga tttaccaaaa agtttacaag
     3121 ataaattgaa tgttccgctt gtatttatag gtgttttttt aggttcaata gtttttctag
     3181 caataacgta cttattgaac tttttaaaag ctttgttagt tgcaatcgga cagtcagaaa
     3241 gtgggctact tggtgtagag ttcgatccta agtttagttt ggcatatcta aaaccacttg
     3301 gggatacgaa acaatacttt atgtgtgctg tggttgcggt aataggagtc gcttatttaa
     3361 tatatgggaa attaaatcat gaaagtgaga aaaatcttgt ttacggtcaa aaaggagatt
     3421 caagactttc aactatcaaa gaaattaaga gagattacca tgagatacca gaaaaaacag
     3481 aagtgtatga tggtattggt ggtgtgccaa ttagtcacta tcaagataaa tattatattg
     3541 atagagacac agtaaatacc gttgttttgg gagcttcacg atccggtaaa ggggaaacat
     3601 ttgttgttcc tttattagat aatcttagcc gagcaaaaat acaaagtaat atggtcgtga
     3661 atgatccgaa aggagaattg tttagcgctt caaaggaaac acttgaaaaa cgaggttatc
     3721 gtgtattagt gctaaatatt gatgatccac ttgaatcaat gagttttaat cctttacaac
     3781 tagtaattga tagttgggct aacaaggact atcacgaagc tagtaagcga gcgaatactt
     3841 taacttcaat gctctttgct tcaggaatgg gtacagataa tgagtttttc tataaatcag
     3901 ctaaatcagc ggtcaatgcg attattttaa cgattgtaga acactgtttt aacaatgatt
     3961 gcattgaaaa aattacaatg tacaacgtgg ctcaaatgct gaacgaatta ggaagcttat
     4021 tttacactga tccaaagact ggaaaagaaa aaaatgcttt agatgaatat ttcaatactt
     4081 taccacaagg gaatcaggcc aagattcagt atggttctac ttcatttgcc ggtgataaag
     4141 ccaaaggttc gattttatct actgcttcac aaggtattga aatgtttaca agtgatttgt
     4201 tcgggaagtt aacaagtaaa atgtctattg atttgaaaga gataggcttt cctaagtcca
     4261 tacaatttaa agtcaatacg aatttagttg gaaagaggat tagtatttca tttttgagaa
     4321 aagaaaatga tgtaattaga ttaattaaga agtatcgagt gaaagtaaaa gctttagggc
     4381 tatgtgtttt aaattttaat gaatatcttc aggatggaga tatgttggat atacgatatg
     4441 aagaagagaa atcgagagca tggtatcgta ttgaatttcc caaaaaacaa gcaaatagtc
     4501 atgatacttc tttagtcact aaaaaattca agtcaggtaa aaattattct gacttagaaa
     4561 tcagcacctt acgattaaaa tacacggatc agccaactgc tgtgttcatg gttattccag
     4621 attatgatcc gtcaaatcac gtacttgcaa gtatttttat cagtcaactt tatacagaat
     4681 tagcaagtaa ttgtaagcaa acaccggata aaaaatgttt taggcgagtt catttcttgt
     4741 tagatgaatt tgggaatatg ccggctatag acaatatgga tgggattatg acggtatgtc
     4801 taggaagaaa tatgcttttt gacttagtga ttcaatccta tagtcagcta gaaacaagat
     4861 atgataaagc ttttaaaaca attaaagaaa attgtcagaa tcatatttta atcatgtcga
     4921 acgatgagga aacgatagaa gagatttcaa aaaaatgtgg tcataagaca acaataaata
     4981 gaagtgctag ttctaaacat ttagatactg atagctcggt cactagttcg gcagagcaag
     5041 agcgagtaat tacagttgag cgtgctagtc aattgattga aggggaacaa attattttaa
     5101 gaaaccttca tagacaagat acaaaaagaa ataagattag accatttcct atttttaata
     5161 ctaaagaaac aaatatgccg tatagatacc aatttttagc agaagacttt gatactcaaa
     5221 gagatattaa tgagattgat attgcatgtg aacatatcaa tctatcttta caagataacc
     5281 aaattccata tgcaaggttt atcaaagatt taaaaacacg tttggagtat tcaattatta
     5341 ataatattcc tattagtgaa gaagactatc aggattatct aaatttatca ggatcacaac
     5401 aaacaattaa tgaagaagag ctgttaaaac tggtaaatac ggaagtaaaa agtcaagata
     5461 tggaaggtca agttttatca gaacaagaaa tgaaaattaa tatttatcta aaggaaagtc
     5521 aaaaggaagc aatagaatta gtaaaagtaa ttaatggaag gttagctgat gaaaattcag
     5581 cagatatggt tataaaaacc attaattcac aaatccttga tacgcctttc attcaatctt
     5641 taatcaatga ttctatagct attcataatg ttcaagagag taaggatcaa attatgatcc
     5701 tagaacaaca attagctaat gcttatgagt tagcagtgtt taaaacgaaa aattcaatac
     5761 tgaaaaataa aattatcaag ttgaaaaatg ctatgactaa gctagcaaaa gcaatttaga
     5821 gagaagggaa gaaatattga ctgaatctat taattggaga aaactaaatg tgcaatggtt
     5881 aaagtataaa tggccaatca tagtttccct aataatcatt ctatttatgg cctatccgca
     5941 acgagttttt gcgttgccgt ggattacaga caataaagag gtattaaaat ttttaacaaa
     6001 atatcaagat tatcttcagt ttactaaagg tggttttgga agcattgtcg ttcaaagttt
     6061 atcgtggggg ctgattagag gtgcatattt tgtttgtaaa tcagcagaaa accttgttcc
     6121 acaatcgcta agtttgttta gctttgttga aagcagtagt ttaggtgctg tttatcaatc
     6181 tgtattaaac acggtggttg taggattaat gatcctttca ttgatgtttg ttggttacaa
     6241 gatgattact ggaaaaggaa cagtggattt aaaatcggtt ggaatgaacg tagtgatgtc
     6301 tatagcatta atacttttaa tgccaaccat gatttctagt ggtattcaat ttgctaaaac
     6361 attttataag gactctacta cgattacaaa agctgatggg ggcgttgctt ggtcattgat
     6421 tgaggacggt gtaactgatt tagtatatat caataaaaac gatgcttatg atcggttggg
     6481 taaagatggc gtacaaaaaa atgcgttaaa agaaaagaat ttcttttcct acgatttcag
     6541 tgaagtaatc acagttgata cagctaaaga tttagaagat gataataaaa aagctgaata
     6601 tcttcaatct aggttgacac cagatggcga aggaaatatg caagccgtta agattgatga
     6661 tggttggatg tcgatgttct ctgatagttt caaaggaggg tactacaggt ttactaaaaa
     6721 tacttttgca ttgactttag gattactttc gttagctgta gcctatgttt ttagtatttt
     6781 tatcatagtc accgctattt tagaactggc atttaagcgt gtgttagggg ttcttgtttt
     6841 tgcaacagat attga
//
LOCUS       DACENJ010000137         6693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.141, whole genome shotgun sequence.
ACCESSION   DACENJ010000137 DACENJ010000000
VERSION     DACENJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.141"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            45..677
                     /locus_tag="IW748_001981"
     CDS             45..677
                     /locus_tag="IW748_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1104889.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(679..1584)
                     /locus_tag="IW748_001982"
     CDS             complement(679..1584)
                     /locus_tag="IW748_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1104890.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1669..2322)
                     /locus_tag="IW748_001983"
     CDS             complement(1669..2322)
                     /locus_tag="IW748_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1104891.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            2644..3588
                     /gene="mvk"
                     /locus_tag="IW748_001984"
     CDS             2644..3588
                     /gene="mvk"
                     /locus_tag="IW748_001984"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1104892.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3581..4558
                     /gene="mvaD"
                     /locus_tag="IW748_001985"
     CDS             3581..4558
                     /gene="mvaD"
                     /locus_tag="IW748_001985"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1104893.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4571..5656
                     /locus_tag="IW748_001986"
     CDS             4571..5656
                     /locus_tag="IW748_001986"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1104894.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5653..>6693
                     /locus_tag="IW748_001987"
     CDS             5653..>6693
                     /locus_tag="IW748_001987"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1104895.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRT"
BASE COUNT         2057 a         1369 c         1341 g         1926 t
ORIGIN      
        1 ttcccgcttc tttgctatac taaagaaaaa cgaggtgaac ccacatgaaa aaaatcgaac
       61 ctattccacc ttctcttcct cttcttacaa agggagagtt ttatttagaa gatgaggaga
      121 tcttctctgg tcttctgatc aacgggggtg accaaagcta tctcgatgcc aaaaatatgg
      181 tattaagaga gagtcatatc caaaaactta cgatgcaaaa aacagtttta cgtcattttg
      241 aatgcagtaa tgtcattttt gaaaaatgtg atttttccaa tctggagtgg attggtggca
      301 gtttccacca agtcgtgttt catcaatgta aattaacagg tacgaatttt gccgaaagtt
      361 atcttcgaga ctgtactttc actgattgta ttgcttcgat ggcttccttt agtagtacaa
      421 acttgaaagc tgtctccttc gatcattgtc aattagaaga cagcgaattt tatgaggtca
      481 cttggaaaaa cctttttttg tctgacaatc agttgaccgg ttcgaactgg tttcgaactt
      541 ctctgaaggg attagacttt acgactaatt tcttcacagc aatcgcttta tctcaagatt
      601 ctttggcggg actaatcgtc aaccaagagc aagcacttgt gatcgcccaa gcccttggtt
      661 tgcagatcaa agactagatc aacgaatccg ccgccacagt gcaataagca tccaaataga
      721 taagaccaaa ctgatgatca aggcgattcc ccatccgaaa acggaatggg taaagggtaa
      781 ctggagattc attccgaaga atcctgtcac aatagttgga acggtcaaga tcagcgacca
      841 gaccgtcaaa aatttcatcg tgtcattcaa gttgttattc agcaaattat tataagtgcc
      901 agagagctgg tctaaaatct gcgaagctaa gttcgtcatt tcaactgctt gtcgtgcttc
      961 gatcagcgca tcgtccaact cttctttttc cttttctgac aacttgcgat aaatagccaa
     1021 tgccttgatt tgttctaaaa gtactacatt ttgttttgtt cctgtaacta agtaaaccaa
     1081 accgatctct aaatctgaaa gttgcagcag attcttgttc gttgtttttt ctcttaattt
     1141 cagattaagc cgctgacgtt cactattcac ttcctcgatt aaaggaaaaa aggaatcaga
     1201 aatcaaaaac aatgtacgaa acaataaact gtatacactc atgtctggca ttcgttccaa
     1261 aagcgatcga attagtcgaa tcacgtactc ggtattcggc gtggtaaatg taaacaaacc
     1321 gtcttctttt aaaataaaag cgataggact agtttcataa tgattatcga ctttttgctg
     1381 gtgcggaaca tttgatacaa gcagtaacgc ttcatcaaaa gaatcgtact ctacgcgcgc
     1441 ccgttcattt ttatctagag aataagtcag catctcgtca ctaatgccat attcttcttt
     1501 taagttttta acagctagca catcgttttg ctgtacattg atccaaaaat tacggtcttg
     1561 attaaaatag tgtttttccc gcatactcta tcctcctgac atttgtttcc agtataaaga
     1621 aaaaagtcgc tcaaataaag gtttaccctt tatttgagca actgtaaatc aaatggctac
     1681 tttggtttgg cgaatctctg ctaatttttc ttcgatcatt ttcaagactt cttcttttgc
     1741 ttgaggatct tccacaaaat tatagcggtc tccatcgatt tggatttttg gactttcttt
     1801 gtattcttca taccattgat cgtaacgtct gttcaattct ttgtaataat cgtatagact
     1861 tggatcaaag gacaattgtt catacgaacg accacgtttt tcgatacgct ccaacatcgt
     1921 atcaaaagat acacgaatat gaactaataa atccggatgt tttttatggg cagcataggg
     1981 aagttcttcc atcatgtttt ccagcagttc atcatacaca cgaacttctg tttctgttgc
     2041 tcttcctaga tctgcattta gatggaaaag cagtgaatct tcataaatcg aacggtccaa
     2101 tacattattg tcatctttca ttgcttgttt gatgcttgca aaacgtttat tcaaaaaata
     2161 aatttgcagc aaaaaagcat actgttcagg gtttgcataa aataagggca agacttcatt
     2221 atcatcaatc gattcataaa atgcctgact atcaaagtgt tcagccatca tttctgttaa
     2281 acttgatttt ccggcaccaa ttgtacctgc taaaacgatc acgctcattt acctccagct
     2341 cacttcatca ctaaatattg tgtttaactc caaccttttt aaatataccc ccaatatcta
     2401 gtagtgtcaa ttgtattttt ttctagtcaa agaagaaaaa aagtcaaaaa attcttaaga
     2461 aaaaagtgtt ttttctttag aaaacgtatt cttagtttta cttctgagat ggttttagta
     2521 aaataaagga ggaatgtttt ttgagaccct tagtggacaa aaaaattcgt gaagcagcgc
     2581 aactcgtttt gggcgcttta ttttttgctt aaaaatcact tttacgtgag aaggagttag
     2641 attatggcaa actatggcca aggagaatca agcggaaaga tcatattgat gggcgagcac
     2701 gcggttgttt atggagaacc agcgattgcc tttcctttct atgcaacaaa agtcaccgca
     2761 ttccttgaag agctggatgc aatggacgat caactggttt cttcctacta ttcaggaaat
     2821 ttagccgaag ctcctcatgc attaaaaaat atcaaaaaat tattcattca cttaaaaaaa
     2881 cagcatgaca tccaaaaaaa cttgcaactg accattgaaa gcacgattcc tgctgaacgt
     2941 ggaatgggat caagcgctgc agtcgccaca gcagtcactc gtgcttttta tgattactta
     3001 gcatttcctt tgtctcgtga aatactatta gaaaatgtcc agctttcgga aaaaatcgcc
     3061 cacggtaatc ctagtggaat cgatgcagcc gctactagca gcttgcagcc gatttatttt
     3121 acaaaagggc atcctttcga ctacttttct ttgaacatcg atgctttttt gattgtcgct
     3181 gatacaggaa tcaaaggaca aacaagagaa gccgtcaaag atgtcgctca cctctttgaa
     3241 acccagcctc atgaaactgg acaaatgatc caaaaattag gatacttgac gaagcaagca
     3301 aaacaagcaa tcattgaaaa ttcaccagaa acgttggcac agacaatgga tgaatcacaa
     3361 tcacttctgg aaaagctgac aatcagcaat gattttctta atctattgat ccaaacagca
     3421 aaagataccg gggcattggg tgccaaatta actggcggtg gacgcggtgg ctgtatgatt
     3481 gcattagcac aaacaaaaac aaaagcccaa gaaatcagtc aagcacttga agatgcgggt
     3541 gctgctgaaa cttggataca aggattagga gtacatacct atgtttaaag gcaaagcacg
     3601 cgcatatacc aacattgctc taatcaaata ttggggtaag aaaaatgaag aactcatcct
     3661 tccaatgaac aacagccttt cgttaacatt ggatgcattt tatacagaga cagaagtcat
     3721 cttttctgat agctatatgg tagatgaatt ttatctagat ggcaccttgc aagacgaaaa
     3781 agcaacaaaa aaagtcagtc agtttcttga cctttttcgt aaagaagccg gcctctcact
     3841 aaaagcttca gtaatcagtc agaatttcgt tcctaccgca gctggattag cctcctctgc
     3901 cagcgggcta gctgctttag caggcgcttg caatactgct cttaagcttg gattagatga
     3961 tctctctctt tcaagatttg ctcgacgcgg gtctggttca gcttgccgaa gtattttcgg
     4021 tggtttcgtc gaatgggaaa aaggccatga cgacttaagt tcttacgcta agccagtccc
     4081 ttccgattct ttcgaagacg atttagcaat ggttttcgtt ttgatcaacg accagaaaaa
     4141 agaagtgtcc agcagaaatg ggatgcgtcg gacagtcgaa acatccagtt tttatcaagg
     4201 ctggttagat tccgttgaag gggatctata tcaattgaaa caagcaatca aaacaaaaga
     4261 tttccaactt ctcggagaaa cgatggaaag aaacggacta aaaatgcatg gaacgacttt
     4321 agctgcccag cctccattca cttactggtc tcctgacagc ttaaaagcga tggatgcggt
     4381 gcgacaattg agaaagcaag gcattccatg ctactttacg atggatgctg gccctaatgt
     4441 caaagtttta gtggaaaaca gccatttatc tgaggtacaa gaaacattta ccaaattatt
     4501 tagcaaagaa caagtaatta ctgcacatgc tggtccagga attgcgatta ttgaataaag
     4561 ggagattttt atgattgaag tatctgcacc aggcaaactc tatattgccg gagaatatgc
     4621 agttgttgaa acaggccatc cagcagttat cgctgcagtc gatcaattcg tgacagtcac
     4681 tgtagaatct gcacgaaaag tcggaagtat ccaatctgca caatatagtg ggatgcctgt
     4741 acgttggaca agacgcaacg gagaattagt tttagatatt cgggaaaatc cttttcatta
     4801 tatccttgct gctattcgct tgactgaaaa gtatgcacaa gaaaaaaaca ttcttttatc
     4861 cttttatgat ctgaaagtaa caagtgagtt agacagttca aatggccgga aatatggatt
     4921 gggttctagc ggtgccgtga ccgttgcaac ggtcaaagcc ttgaatgttt tttacgcgtt
     4981 gaatttatct cagttggaga ttttcaagat tgcagcacta gccaatttag cagttcaaga
     5041 taatggttcc tgcggcgaca tcgctgctag ctgttatggt ggctggatcg ctttctcaac
     5101 cttcgaccat ccttggctcc aagaacaaga aactcagcat tctatcagtg agttacttgc
     5161 cctggattgg ccaggtctat ccattgagcc attgattgct cctgaagatt tacgtttatt
     5221 gattggttgg acgggtagcc ctgcctctac ttctgatttg gtcgatcaag ttcaccgttc
     5281 gagagaagat aaaatggtgg cttatcagct tttcttaaaa aatagtacag aatgtgtcaa
     5341 cgaaatgatc aaagggttta aagaaaataa tgtaacgttg atccaacaga tgattcgaaa
     5401 aaaccgacaa ttactgcatg atttatctgc aatcactggg gtcgtcatcg aaacgcctgc
     5461 tttgaacaaa ttgtgtaatt tagctgaaca gtatgaagga gccgcaaaat cttctggtgc
     5521 aggtgggggc gattgcggaa tcgtaattgt tgaccagaaa tctggcattc ttcctttaat
     5581 gagtgcatgg gaaaaagcag aaatcactcc actgccgtta catgtctata gcgatcaaag
     5641 aaaggaaaac cgatgaatcg aaaagacgaa catgtttcac tagctaaagc ctttcatgat
     5701 aaacaaaaaa acgaatttga cttcgtacgg atcatccata accctttgcc gcaaatagca
     5761 gtttctgatg tcgatctaag tacacaggca gttgggttta cactaagcag cccattttat
     5821 atcaatgcga tgactggcgg aagcgaaaaa acaaaaaaaa tcaaccaaga tcttgcaatc
     5881 gtcgcaagag aagcagattt gatgattgcc actggttcag tcagcgctgc cttgaaagtc
     5941 ccttctttag ccgatactta tacgattatg cgacaagaat atcctcacgg gaaaatcatt
     6001 gccaacatcg gtgcagggac ttctgtcgaa agagccaaag aagctatccg attgtttcac
     6061 gcagatgcct tacagattca tttgaatgca cctcaagagt tggttatgcc tgagggagac
     6121 cgtgatttta ccaattggaa agtacttatt caagaaactc aaacagccat tgacgtcccc
     6181 ctcatcgtaa aagaagtcgg ttttggcatg acaagagaaa cgctcaatga tctagctgct
     6241 ttaggagttc atacagttga tatcagcggt cgaagcggaa caagcttcac ccagatcgaa
     6301 aacgcccgtc gctccaagcg ggaactgagt tatttagctg actgggggca atcgacagtc
     6361 tcttcattgc ttgaagcaaa tgaagcagac acttctatgg aaatcctagc ctccggaggt
     6421 atccgcaatg cctatgacat atttaaagcc ctttgtctcg gcgcaaatgc cgtgggtact
     6481 tcaggaaccg tactgacgca tctgatgaat cacggcgtag aagaaactat tatactgatg
     6541 aagcaatggc aagaagagct tcgtctgctt tatactatgg tcggcgcaac gaataccgcg
     6601 gccctacacc aacaatctct gatcttttcc ggaccagtga aggattggtg cgaagcaaga
     6661 gggatcgact tagtgaagta tggaagacgc aca
//
LOCUS       DACENJ010000138         6543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.142, whole genome shotgun sequence.
ACCESSION   DACENJ010000138 DACENJ010000000
VERSION     DACENJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.142"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..269
                     /locus_tag="IW748_001988"
     CDS             <1..269
                     /locus_tag="IW748_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="modulates transcription in response to the
                     NADH/NAD(+) redox state; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1104896.1"
                     /translation="IDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYSQSAIDEIVKGN
                     VTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            662..3307
                     /locus_tag="IW748_001989"
     CDS             662..3307
                     /locus_tag="IW748_001989"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1104897.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3617..4930
                     /locus_tag="IW748_001990"
     CDS             3617..4930
                     /locus_tag="IW748_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1104898.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            4920..5573
                     /locus_tag="IW748_001991"
     CDS             4920..5573
                     /locus_tag="IW748_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1104899.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            5628..6299
                     /gene="radC"
                     /locus_tag="IW748_001992"
     CDS             5628..6299
                     /gene="radC"
                     /locus_tag="IW748_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ1104900.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
BASE COUNT         2121 a         1097 c         1407 g         1918 t
ORIGIN      
        1 taattgatgg agaaatgatc tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa
       61 atgtgacagt agcaatctct acggtaccaa gtaaatattc acaatctgct attgatgaaa
      121 ttgtcaaagg aaatgtaacc gctatactta attttgcgcc tgacagagta actgtcccag
      181 cttatgtcaa tgttcaatac attgatttga caaccgagtt gcagacgctt atttactttg
      241 atgagaatta ctcagaagtt ttttcttagg aaaatttgag taggcttatt gatcttgcga
      301 gaactcgtcg taattctttc aaaaattttt tagtactaaa tgaagtggtt ttttcacgac
      361 cacttgttga ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga acaagaggag
      421 taactggcac attcattctt agagagagaa ctatctgctg aaaggttctc aatgacaacc
      481 agtgaaggta gcttgggagt ttctgtcttg aatggagtag agatagacga gtcgtgatcg
      541 ttaactcact tgagaggcaa taatgattga ttattgcaat ttaggtggta ccgcgtaaat
      601 catacgtcct agaatacgaa agtattttag gacgtttttt agtttaaagg aggaaaacag
      661 catgacggaa gaaaataacc tatcaacaaa atataatccg caagaagttg aagctggacg
      721 ttatcagcat tggttagaac aagatttgtt taaaccgagc ggagataaaa aagcaaaacc
      781 ttattcaatc gtaatcccgc ccccaaatgt tactggaaaa cttcatttag ggcatgcttg
      841 ggatacgacc ttgcaagata tgctgattcg tcaaaagaga atgcaaggat atgatacttt
      901 atggctgcct ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct
      961 tagagagcaa gggatttctc gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg
     1021 ggaatggaaa gaagagtatg cgagccacat ccgcgaacag tgggctaaaa tgggcttatc
     1081 tttagattac agtagggaac gttttacatt agatgatgga ttatctgaag cagtacgtaa
     1141 agtttttgtt accttatacg aaaaaggttt gatttatcgt ggtgaatata tcatcaactg
     1201 ggatcctcaa gcacgaacag ctctatctga tatcgaagtt attcataaag atatcgaagg
     1261 cgccttttat catatgagct atgaactagc agatggatca ggcgtagtag aaattgcgac
     1321 aacccgtcca gaaacgatgt tgggagatac ggcgattgct gttcatccag aagacgagcg
     1381 ttatcaagcg ctcatcggca aaaaagtgat tctaccttta gtcaataaac aaatcccaat
     1441 cattgcagat gaatatgtag atatggaatt cgggacaggt gtagtaaaaa tcactccagc
     1501 acatgatcca aacgactttg aggtaggtaa tcgtcatgat cttccacgcg taaatgttat
     1561 ggatgatgat ggtacgatga atgaactagc aggaaaatat gctggaatgg aacgttttgc
     1621 tgctcgtaaa gcaatcgtgc aagatctgaa agaaatcggt cgattgatta aaatcgatac
     1681 gatgatccac agtgtgggtc attccgagcg gacaggtgtc gttgttgaac ctcggctgtc
     1741 tactcaatgg tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata accaagaaac
     1801 agatcaagca gtggaattct tccctccaag attcaatcaa acgtttttac gttggatgga
     1861 aaatgtccat gattgggtaa tctcacgaca attatggtgg ggacatcaaa ttcctgcatg
     1921 gtatcacaaa gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa
     1981 ctgggtacaa gatcctgatg ttttagacac ttggtttagt tcagctttgt ggcctttctc
     2041 aacaatgggc tggccagata cggaaagcga agattataag cgatacttcc ctactagcac
     2101 tttagtaacc ggttatgaca tcattttctt ctgggtaagc agaatgattt tccaaagctt
     2161 ggaatttact ggcgaacgtc catttgagaa tgtcttgatt catggattga ttcgtgacga
     2221 acaagggcgg aagatgagta aatcattagg taatggaatc gatccgatgg aagtaatcga
     2281 aaagtatgga gcagatgctc tccgctggtt cttatcgaat ggttctgcac caggacaaga
     2341 tgtccgcttc agctatgaaa aaatggatgc ttcttggaac tttatcaaca aaatctggaa
     2401 tgcttcacgt ttcgttatca tgaatatcga aggaatgacc gtagaagaga tcgatttatc
     2461 tggagaaaaa tcagtggctg accggtggat ccttactcgt ttgaatgaaa cgatcgaacg
     2521 agtaactgag ctatttgatc gttttgaatt tggtgaagca ggtcgacaac tgtataactt
     2581 tatctgggat gatttttgtg actggtacat cgaaatgagt aaagaagtac tttatggaga
     2641 tgatgctgtt tcaaaacaaa ccacccgtag catcttagtc catacacttg atcagatcct
     2701 acgtttattg catccaatca tgccattcgt tacagaagaa atatgggaac atattcctca
     2761 tcagggaaat tctctagtag tggcagaata tcctgttgta cgtccagagt ttgatgatga
     2821 aacagcttct aaagggatgg aagtattgaa agaattgatt cgagccgttc gaaatattcg
     2881 ttcagaagtg aatacaccat tgtctaaacc aattacgttg ctaattaaaa caaacgatgc
     2941 taaaatcgat caatttttag ttgaaaatac aagctatatc gaacgcttct gtaatcccga
     3001 agagttgacg atatcaagtg agatcactgc gccagatcta gcgatgtcag ctgttctgac
     3061 aggtgcagaa atcttcttgc cgttagcagg attgatcaat attgaagaag aaatcaaacg
     3121 attagaaaaa gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc
     3181 taatgaacgt tttgtttcta acgcgccgga agaagtagta gaggcagagc gtgcaaaaga
     3241 aaaagattat ttagagaagc aaacggctgt caaagaacga atcgaaagtt tgcgtactat
     3301 tcaataagaa tagaaaaaga gagtgtgaaa tcagctagct attactaaaa gctagctgat
     3361 tgaacatcgt tacaggaaag agtttgagcc tcactcttag cttcgattca taaggagatg
     3421 tgacataaat tttgtcgcat ctctttttta gtctactctt attatgtagg agaaggaggg
     3481 aggtggatca tgtatcaaga gtcaataaag tagttgataa aatcgttcct ctctctgttt
     3541 cttttctctt tgtcatactt ttatttttct tatgtgttct atttgttata ataaaagaga
     3601 aatgggggaa ttgttgatga cgattgaaga agcaatagaa tggatccata gtcgattacc
     3661 atttggttca cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc
     3721 agagagaaaa gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc
     3781 ttatttgaga agcctattag aagaggctgg gctgacagta ggtacattta cttcacctta
     3841 tatcgaaacg ttcaatgaac ggatcgcaat caatggcaaa agcattccag acaatgattt
     3901 gatccagctt gtaaaaaaat atcagccact gatcaaacaa cttgatcaat ttgaagcagt
     3961 agctggtatt actgaattcg agacattaac agcaatggca tttgattatt ttttgatgaa
     4021 gaaagtggac gtagcgatta tcgaagttgg cttaggcggc ttattggatt ctaccaatgt
     4081 agctgtacca atgctgacgg gaataacaac gattggactt gatcataccg aaatacttgg
     4141 agatacaatt gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag gcgttccagt
     4201 agtaacagga aatattgccc cagaagcttt tactgtcatc caacaaacag ctaaagcaaa
     4261 aaatgcaccg ctttatgtat acggggaaaa ttataaaata aactatctgc atcctgatga
     4321 gatgtgggga gaagtatttg aattttttaa tgaaatagga aagatccctc aattaaccac
     4381 tccgctttta gggcaacatc aagctgaaaa tgcaggagta gccatcgaat tgtattttgt
     4441 ttactgtcaa ttggaaaagc tgcctttccg agaaaaagat gttttgaagg gattgaagaa
     4501 atctcgatgg ccaggaagaa tggagcgttt gagtaacgag ccattagttg ttttagatgg
     4561 tgcccataat acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg
     4621 atatcaaatc aacattttgt tttccgcctt acgaactaaa aatgttagag aaatgctaga
     4681 acgattgcag caagtaccaa atgtccatat ttatttgacg acctttgaac atccaaaagc
     4741 catcgaatta agtgaagtat cagatttagc aagtgagaga gtatcaatcg tttcattgtg
     4801 gcaattcgga ttagcagata tattggaaaa gatgacaaat gaagacgtat tagttgtgac
     4861 cggttcactt tatttcattt cagaagttcg ccatttatta atacaattag gaggagatca
     4921 tgaagtataa tgcagtgatt tttgacatgg atggtttgct gttcgacaca gagttagtgt
     4981 attacgaagc cacacaagta gtagcagaca agatgggttt tccttatgat aaagacctgt
     5041 atttaaaatt cttaggtgtt tcagatgaag aagtatgggc gaattatcat atgattttct
     5101 cggaatttgg caaaacaagt gtacaaagat tcatagatga ttcataccag gaaacgcttg
     5161 aacgtttttc gcaaggagcg gtacaaatga agcccggtgt gttggaactc ctctcttttt
     5221 tagaagaaca tcagattccc aaagttgtcg cttccagtaa ccaacgagca gtcattgaac
     5281 ttttattgaa gaagaacaat ctgtttgaac gatttgagac gattgtttct gcagaaaatg
     5341 ttcaacgagc aaaaccagac ccagaaattt tcttgttagc taatcagaag ctaggtacag
     5401 cgaagaaaga aacattgatt ttagaagatt cacaaaatgg tattttagca gcttctgcgg
     5461 ctgagatccc ggtagtcatg gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa
     5521 aaacagctgg ggttgtttct tctttacatg aaatccctgc attgttaaaa taaaaatcca
     5581 tctcttttcg tatcttattt tagataagat agaaaggaga tgtttttttg ggaaaacgat
     5641 tgatcaaaga agtacctact agctctttac caagggaacg tatggaaatt tatggcgcgg
     5701 aagctttatc ggatcaggag cttttggcaa tcttactgcg taccggtcaa cacccttata
     5761 gtgtgatgtc gattgctgga aatctgttaa aaacgtttgg aggactggcc tctttacgac
     5821 aggctacttt gcatgagtta gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa
     5881 tcaaagcctt gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac
     5941 cggttcgtac cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac
     6001 aagaacattt ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag
     6061 tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg
     6121 tacgttattg tgcagcaagg atcgttttag cgcataatca tccttccgga atatataagt
     6181 tagtacacat aaattatata aggaaattta ctgttatatt gccatctgga aatactatta
     6241 tgttatcgat aattgaacgc cataatgcat ttttttcttc ttccgaagat tctttataaa
     6301 tttcatcgaa ttcttttgat agaataaatt cgacattttt tatatctatt ttagctggct
     6361 ctacttcaat ttcatttaat tctttcatta aagcttctcg atcttttttg tattcctcta
     6421 agtctataac ttcattcaag taggctttct tcaatctatc taatttattc tcaatatttt
     6481 tcttactatt tttcttttta ataatctttg gttgtttttt tgaatagttt atttgtattt
     6541 gtt
//
LOCUS       DACENJ010000139         6484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.143, whole genome shotgun sequence.
ACCESSION   DACENJ010000139 DACENJ010000000
VERSION     DACENJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.143"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            146..1312
                     /locus_tag="IW748_001993"
     CDS             146..1312
                     /locus_tag="IW748_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1104901.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1547..2590
                     /gene="hrcA"
                     /locus_tag="IW748_001994"
     CDS             1547..2590
                     /gene="hrcA"
                     /locus_tag="IW748_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1104902.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2684..3247
                     /gene="grpE"
                     /locus_tag="IW748_001995"
     CDS             2684..3247
                     /gene="grpE"
                     /locus_tag="IW748_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1104903.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3298..5127
                     /gene="dnaK"
                     /locus_tag="IW748_001996"
     CDS             3298..5127
                     /gene="dnaK"
                     /locus_tag="IW748_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1104904.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5278..6444
                     /gene="dnaJ"
                     /locus_tag="IW748_001997"
     CDS             5278..6444
                     /gene="dnaJ"
                     /locus_tag="IW748_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1104905.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2223 a         1125 c         1463 g         1673 t
ORIGIN      
        1 ctccgatgac aataagcaag catttttaga gctaggtttc cttgattcta aatatgcggc
       61 ttatttcaat gagttggtgc ttgaacgcga ttttaagaag gtcgaaaaca ggcacgactt
      121 ttcgacgtaa ggagaaagaa tttcaatgat agaaacaata aatcccgcag aaaacaaggt
      181 ttctgcgtac attcatatcc cattttgcga gcatatctgc tattactgtg actttaataa
      241 agttttttta gaagggcagc cagtagatga gtatgtggaa atgctgctga atgaaatgag
      301 attaatgctg gaaagtcatc ctacagcgaa tttggaaaca ctttatgtag gaggcggaac
      361 accaacttct ttatctgcta aacaacttga tcgactatta tcaggtgcta gagagatcct
      421 gccttttcaa aatggtaatg agtttactgt agaagcgaat cctggtgatt tgacccgtga
      481 aaaacttcaa gtgatgaaaa actatggcgt caatcgttta tccatgggag tgcagacatt
      541 tgacaatcgc ttattgaaaa aaattggacg taaacatact gcagaagatg tgtatcaaac
      601 aatgcaattt ttagaagcag aaaattttac caatgtaagc atcgatttga tttatgcatt
      661 accgggacaa acactagaag gatatcggga tactttggaa aaagctttgg ctttggatct
      721 gcctcactat tctttgtact cattgatcct cgaaaacaaa acaatgttca tgaactgggt
      781 acgacaagga agactcgaac tgcctgatca ggaaacggaa accagaatgt ttgaagagac
      841 gattgaagca atggaaaagc atggacgtca ccaatatgaa ataagtaact ttgggctctc
      901 cggacatgaa tctcaacata acctcatgta ctggaacaat gaccactatt ttgggtttgg
      961 agctggtgct agcggttatc ttggagatat tcgttaccgc aacaaaggac cgatccaaca
     1021 ctatttacgc ccattacgag caaatgaacg cccggtattg gaagaagaaa aattgacacg
     1081 aaaaaatcaa atagaagaag aaatgttctt aggactaaga aaaaaaacag ggattactaa
     1141 aaaacatttc tatgatcgct atcaaaaaac aattgaatcg gtgtacggtg atgtgctaag
     1201 ggaattagag caagaagggt tacttgttaa tgaagaagac cgtgtttatt tgacaccaag
     1261 agggactttt ttggggaaca atgtgtttga gcgcttttta tttgaaaaat agtttgaaga
     1321 atatttgttt tttatcgaaa cttgaaaaga aaaagtttag aaagtttgac aatgaggtat
     1381 tgaagtattg atataactgg atttacatca attatttaga attagcactc ttgtgtttag
     1441 agtgctaaaa aatggagtct ttctcttgac aggagaaggg cttcttgcta tatttataag
     1501 tgaattagca cttagaatat atgagtgcta atgagaggtg aggaagatgt taacaaagcg
     1561 acaaaacgac attttacgtc tgatcatcca acactatacg agtagtggcg tgcccgttgg
     1621 ttccaaaaca ttaatgtctg agggagtcaa ggccagctca gcaacgattc gcaatgatat
     1681 gaaagcatta gaagatgaag gattgctgtt gaagactcac tcttcttctg ggcggattcc
     1741 atctgcttta ggctatcgtt attacgtgga tcatttgtta aaacctgctc gcgtagcaga
     1801 agatgatttg ctgctgattc gtcagtctct aggcagagaa tttcatgaaa tcaatgagat
     1861 tattaagcaa tctgctgaga tattatctga gctgacaagc tatacgactt tttcattggg
     1921 acctgaaata aaagaccgcc gactgactgg ctttcgtatc gtacccttga ataatcgaca
     1981 gattattgct atcgtcgtaa cagatatggg gaatgtagaa agccaagtgt tcactcttcc
     2041 cgaatatatg ggaagtcagg aactagaaaa aatggtacag attgtaaacg accgtctagt
     2101 cggagatcct ctgatgacgg tgtaccataa acttcgtacg gagattccga tgattcttca
     2161 taaatatttc cagacgacag aagggatgtc tcagttattt gacaccgttc ttggtcatgt
     2221 ttttgaagaa aaggtttatg tcagtggaca aatgaatctc ttggattatg agccccgtca
     2281 agatcttgat cagttcaaat cgatgttttc ttttatgaaa gactcagacg gacttacaca
     2341 aatgatcact ccgatggata gcaatattca tatcaagatt ggtgctgaat taggcaatga
     2401 tttattgcag aatatgagta tgattcaagc aagttacgag atagatggtc atggaagagg
     2461 tacaatagcc ttgttagggc ctactagtat gccttattct cggatgttcg gtctagtcga
     2521 tgtttatcga agagaactag cagctaaact tgctgattat taccgctcgc ttgacttgtc
     2581 aaactcgtaa tgtgaataac gaagctattt gcagctgatc gttctctctt tcttagtaag
     2641 agcaatcaaa cagctgactg aataatgaaa ggaagactgt atcgtgaaag aaaatcaaga
     2701 agaactagat aaagaaatga cagatgctca gccagaaccg gaaatcgatg tcgaagctgc
     2761 agaagattcg ggtatttcag aagctgaagc ggaagaattc gaaacagcga aattaaaagc
     2821 tgagttagaa gaaatggaag atagatactt gcgggctcgt gctgaaatcg caaatatggc
     2881 aaatcgagga aaaaatgagc gtgagcaatt acaaaaatac cgttcacaag atttagccaa
     2941 aaaattactt ccatcaattg acaacctgga acgtgcatta gccacagaag tgtcagatga
     3001 tcaaggggca ggtttgaaaa aaggcgtgga aatggtcttg gaaagtttgc gtaacgcact
     3061 ggaagaagaa ggaattgaaa aaatccctgc taaaggagaa gcctttgatc ctaatcttca
     3121 tcaagcagta caaactgttc cagcgactga agatacacca gccgatacga ttgtagaagt
     3181 actacaagaa ggctacaaat tacatgatcg cgtgttaaga ccaactatgg tcattgttgc
     3241 acaataattg tgcggatata aaacttacac tttcaattaa agaaggagac gttaattatg
     3301 agtaaaatta tcggtattga cttaggtacg actaactctg cagtagcagt attggaagga
     3361 aacgaagcaa aaatcatcgc taacccagaa ggaaaccgta caacaccttc tgttgtatca
     3421 ttcaaaaatg gagaaatcca agtaggggaa gttgcaaaac gtcaagctgt gacaaaccca
     3481 aatacgattt catcaatcaa acgccatatc ggtgaagcag gttacaaagt agatgtagaa
     3541 ggtaaatcat atacaccaca agaaatctca gcaatgatcc ttcaatatat caaaggattc
     3601 gcagaagact atttaggtga aaaagtcgaa aaagcagtta tcactgtgcc agcttacttt
     3661 aatgatgcac aacgtcaagc aacaaaagat gctggtaaga tcgctggtct agaagtagaa
     3721 cgtatcgtca atgaaccaac tgcagctgct cttgcctatg gtttagataa aacagatcgc
     3781 gacgaaaaaa tcttagtatt cgaccttggt ggcggtactt ttgacgtatc tatccttgaa
     3841 ctaggagacg gtgtctttga tgtattgtca acagctggcg acaaccatct aggtggggac
     3901 gactttgata acaaaatcat cgactacatg gtagcagaat tcaaaaaaga aaacagcatt
     3961 gacttgtctc aagacaaaat ggctttacaa cgcttgaaag acgctgctga aaaagcgaaa
     4021 aaagatttat ctggcgtatc aagcacacaa atcagcttgc catttatcac agcaggagaa
     4081 tctggaccat tgcatttgga aatgacattg acacgtgcaa aatttgatga attaacagct
     4141 gacttagtag aacgtacaaa agtaccagta cgtcaagcgt taaaagatgc aggtctgtca
     4201 caatcagaaa tcgacgaagt tattttagtc ggtggatcta ctcgtatccc tgcagttgta
     4261 gaagcagtac gtaaagaaac aggaaaagaa ccaaacaaat cagttaaccc tgatgaagta
     4321 gttgctatgg gtgctgctat ccaaggtggc gtgatcactg gtgatgtgaa agatgttgtt
     4381 ttacttgacg ttacaccatt gtcattaggt atcgaaacaa tgggtggagt gttcactaaa
     4441 ttgattgacc gcaatacaac gattccaaca agtaaatcac aagtattctc aactgcggca
     4501 gataatcaac cagcagtaga catccatgta ttacaaggtg aacgcccaat ggctgctgac
     4561 aacaaaactc taggaagatt ccaattgaca gatattccag cagctcctcg tggtattcca
     4621 caaatcgaag taacattcga tattgataaa aacgggattg taaatgtatc tgcgaaagat
     4681 ctaggtacac aaaaagaaca aaaaatcacg atcaaatctt cttcaggtct aacagatgaa
     4741 gaaatcgaac gtatggtgaa agatgcagaa gcaaacgctg aagcagataa agctcgtaaa
     4801 gaggaagttg acctacgtaa tgatgtagac gcattgctat tctctgtgga caaaacattg
     4861 aaagaattag aaggcaaagt ggacgaagaa gaagtgaaaa aagcagaaac agctcgtgac
     4921 gaattaaaag ccgctgttga agcaaacaac atcgaggaaa tgaaaactaa acgcgatgca
     4981 ttgaacgaaa tcgttcaaaa cttgactgtt aaactatacg aacaagctgc acaacaacaa
     5041 gctcaagaaa atccagaagc agctcaaggc ggagcagatg atgtcgttga tgctgatttt
     5101 gaagaagtcg atggcgacga taaataatca gttcactgaa tttcgagaga aggccaaagt
     5161 tgctactttg gctttttctt ttgaaaaata gtttgaagta tcggtttttg tttcaaacaa
     5221 aatatgatat gataacaagc gacgtgaaaa tgatgagaac caataggagg aaagcctatg
     5281 gcaacaaaac gtgattatta tgaagtctta gggctctcaa aaggagcatc agaagatgaa
     5341 ataaagaaag cttatcgtaa gctttcgaaa aaataccatc ctgacatcaa taaagaacct
     5401 gatgcggaag aaaaattcaa agaagtatca gaagcatatg agattttaag tgacccgcaa
     5461 aaacgcgctg catacgacca atacgggcat gcaggcacag atccaaatta cggtgctggt
     5521 ggagctggtg gcggatttgg tggattcggc ggcttctctg gtggcggctt cggtggtttt
     5581 gaagatatct tcgactcttt ctttggagga ggcggtcgat cagtcgatcc aaatgcgcct
     5641 cgtcaaggtg ctgatttgca atatacggtc gacttgtctt ttgaagaagc gatttttggt
     5701 gtagaaaaag aaatcaagta taatcgggaa gaaatctgtc atacttgtgg tggaaacggt
     5761 gcaaaaccag gcacacaacc aactacttgt cataaatgtc atggtgcagg tacgatcaat
     5821 gtagaacgcc aaacgccact tggacgagtg atgagccgtc aaacttgtga tgtttgtcat
     5881 ggcacaggga aagaaatcaa agaaccttgt ccaacctgtc atggctcagg acatgagaag
     5941 aaagcacatt cagttaaagt gaatgtacca gctggtgtag aagaaggaca acaaatgcgt
     6001 ttagctggtc aaggagaagc tggagaaaac ggcggaccat ttggtgattt gtatgtggtc
     6061 tttcgtgtag aagaaagtga tatctttgat cgtgaaggtt ctgaaattta ctatgaattg
     6121 cctttaagtt ttgtacaagc agctttgggg gatgaagtcc aagtaccaac tgtccatgga
     6181 gatgtgaaat tgaagatccc tgctggaaca cagacaggta cgaacttccg tttacgtgga
     6241 aaaggcgctc cgaagttacg tggtggtgca acaggcgacc aacatgtcaa agtaaaactg
     6301 atcacaccga aaaatctaaa tgaacaacaa agagaagcat tacgtgcatt cgctgaagct
     6361 ggcggcatca aagttgaaga acagcaagaa gacggtttct tcgataagtt taaagatgct
     6421 tttggcggga aaaagcggaa gtagtttttc tagtaaaaat ataaagtaga cttgcccaaa
     6481 tttt
//
LOCUS       DACENJ010000140         6392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.144, whole genome shotgun sequence.
ACCESSION   DACENJ010000140 DACENJ010000000
VERSION     DACENJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.144"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(247..435)
                     /locus_tag="IW748_001998"
     CDS             complement(247..435)
                     /locus_tag="IW748_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1104906.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            705..1067
                     /locus_tag="IW748_001999"
     CDS             705..1067
                     /locus_tag="IW748_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1104907.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1119..2801
                     /locus_tag="IW748_002000"
     CDS             1119..2801
                     /locus_tag="IW748_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1104908.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2918..4954
                     /gene="recG"
                     /locus_tag="IW748_002001"
     CDS             2918..4954
                     /gene="recG"
                     /locus_tag="IW748_002001"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1104909.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            5003..6004
                     /gene="plsX"
                     /locus_tag="IW748_002002"
     CDS             5003..6004
                     /gene="plsX"
                     /locus_tag="IW748_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1104910.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6126..6368
                     /gene="acpP"
                     /locus_tag="IW748_002003"
     CDS             6126..6368
                     /gene="acpP"
                     /locus_tag="IW748_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1104911.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2140 a         1106 c         1438 g         1708 t
ORIGIN      
        1 ttttttcata tttattttcc accaaatttc tatgaagttg tattcagttt atcatatcct
       61 tgtttttctc tgcttaaaaa gagtctagtt ttcacaaaaa aataaaattt agaataaata
      121 acttcataat ttgttcgtat aacaaaaata aacgcaaaaa aagggtagga atcaaaagtc
      181 ccacccttaa aaatttttat taaataaaaa accgttcact atctgaacgg tccaagtatc
      241 attcaattaa acgcgttcaa cttttcctga tttcaaagca cgagttgaca cccaaacttt
      301 tttaggtttt ccgtcgacca taacgcgaac tttttgtaag ttaggtttaa cagtacgttt
      361 tgttgagttc atagcgtgtg aacggttgtt tccacttttt gtcttgcgac ctgtaaagta
      421 acaaactttt gccatggtaa tttcctcctt tgccttgatt tggagcaata gaataattca
      481 gctcatacca gactaacata tcatatagaa tgggaatttg caagtacatt ttcatagata
      541 caattgacaa ttgagaagaa taattgtaaa ttaaacaagg ttatacctga aattttgcaa
      601 ggtttttgag tactttgggt aactgccttt tatttcaagt tgcgctatga taaaatatga
      661 tagaagaaaa atgggagttt tttacccgtt aaggaggctc attcatggct gtaaaaattc
      721 aaacgtcagc tggaaccatt gaaattacca atgatgtaat tgcaacagtt gttggtggcg
      781 ccgcgacaga tgtgttcggt gttgtaggaa tggccagcaa aaatcaaatt aaagataata
      841 ttactgagat tttgcgtaaa gaaaattact caagaggagt agttgtccgc caagaagata
      901 atggtattgc tgtagatgtt tatacgatta tcagctatgg tacgaaaatc tctgaagttt
      961 cccgcaacgt ccaagaaaaa gtgaaataca atctagaggc attattaggc gtaactgcca
     1021 attcagttaa tgtctttgta caaggtgttc gtgtactgcc agactaggca ggagactggc
     1081 tggtaactca gcagtcgatc tgaaggagga ttatctaggt gagtgtaaca gaaataagtg
     1141 caagccagtt ccaggaaatg gtccaggcag gtgcgaatcg tctgcaagta aatgcagaat
     1201 acgtcaattc attaaatgta ttccctgtgc cagatggcga tacgggaaca aatatgaatc
     1261 tatctatgac tagtggagct aaggcagtag tagattcaac ctcagaaaaa gtaggagaat
     1321 tagcagcaat gctttcaaaa ggattgctga tgggtgcgcg cggaaattcc ggcgttattt
     1381 tgtctcaatt gtttcgtgga ttttctaagc aaattccaga tgttgtctca ttaaatgctt
     1441 ctgatcttgc tgcagccttc actcatggag ttgaaactgc gtataaagca gtaatgaaac
     1501 cagtagaagg aacgattttg acagtagcta gagaagcagc gaaagctgga gaaaagaaag
     1561 caaaaagtac tgatgatgtc gttgaagtaa tgacagctgt tgtaaaagga gcaaagcgtg
     1621 ctttagctaa aactccagac ttgcttccag tcctaaaaga agtcggtgtc gtagacagtg
     1681 gagggcaagg actgctgttt gtttatgagg gctttttaag tgcattgaat ggagaattcc
     1741 aagcggatga aacatatgaa ccttctccag cagaaatgga tgatatggtc aatgcagaac
     1801 atcatcgcag tgtccaaggt caaatggcga cagaagatat ccattttggt tactgtacag
     1861 aaatcatggt aaaaatcgga gaaggaccta cagtcgatag tacattcgat tacgaagaat
     1921 tccgtaacta tttagatggt ataggagatt ctttacttgt ggtcaatgat gacgagatca
     1981 tcaaagtcca cgtgcataca gaaaacccag gagaagtctt gaattatgga caaaaattcg
     2041 gttcattagt caaaatcaaa gtcgacaaca tgcgcttgca gcatgagacg attttggaac
     2101 acgatgaaga agtgaacgag tttgcgaaaa aagaaatacc acgtatcccg tatggtatcg
     2161 tagcgatcgc tgctggtaaa ggcgtccaag agctgtttga aagtttaggc gctaattatg
     2221 ttatcagtgg tggacaaaca atgaatccaa gtacggaaga cattgttaaa gcgattcaag
     2281 aagtaaacgc agataaagtg attgtcttgc caaacaataa gaacatcttc atggcagctg
     2341 atcaagcagc agaagtagca gatatcccag tagcagttgt tccttcaaga acgatttccc
     2401 aaggattgac agctatgctt tcattcaatg atcaagcttc tttagaagaa aacaaagaaa
     2461 cgatgacaga aatgttgtct agcgttgtga gcggtcaagt cactcacgcg atcagagata
     2521 cctcaattga cggtgtagag atcaaagaag gagacttttt gggcatgatc gatggaaaaa
     2581 tcctgatctc taacccagat atcacagcaa cagcgttaag tacactagaa caaatgatca
     2641 atgaggacac agagatcgta acgatcttgg caggtgatga aggaacagtt gaacaagcaa
     2701 atgaatttgc tgagaaacta acagaaaaac atcctgattt ggaaatcgag atccaccaag
     2761 gagatcagcc tgtctatcct tatctatttt ctgctgaata atttctgtgt cagaatatct
     2821 ttctaacaca gtattcattc ataaaaggcc ttcggttagt gatcaggtat gatcgctgac
     2881 caagtccttt ttttgttaaa ggagggagga aaaatttatg cgttctttag atgatccaat
     2941 cactatgctg gcaggagtcg gagaaaaaag agcagctagt cttgccagct tgggcatcca
     3001 aacgattgaa gacttactga cttattatcc ttttcgctac gaagatattc aggaaagaaa
     3061 tctaaacgag atccaggatc aagaaaaggt tactttgaaa ggaatcgtag tctcgcctcc
     3121 tgtgatgaat cgatttggtt ataaaaaaag ccgattgcaa tttcgtatga tgcaagaaca
     3181 tgatgtgttc aacgtttctt tttttaatca accttatcta aaagaaaaag tcattttatc
     3241 ggaggaaatc gccgtatacg gtaaatggga tgcaaagcgt aaagccttga acgggatgaa
     3301 gattttaggt tctcaaacaa tggatgactt ttcgccgata tatcacgtca acaaatcaat
     3361 tcgtcaaacg acattagttg atctaatcaa aaaagggttt caagaatacg gcacattgat
     3421 tgaagaaaat ctgccaaaca accttgtgga gaaatatcga ctgcttgacc gagcttctgc
     3481 tgtaagaagt atgcattttc caaaagacca cgaagaaaac catcaagcca aacgacgtgt
     3541 cgtttttgaa gagttctttt tatttcaaat gcgtcttcaa ggattgaaaa aagcagaaaa
     3601 agcagaaacg aatggtttgg agatccttta tgatgtccaa aaattgaaac aatttactca
     3661 aaagctacct tttgaactga caaatgcaca aaaacgtgta acgaatgaaa tctgtcggga
     3721 tcttcgcagt ccgcagcata tgcagcgact tttgcaggga gatgtaggta gcggaaaaac
     3781 agtggttgcc gcgattgcgt tatacgctac cgttacagca ggtttccaag gtgcgttgat
     3841 ggtgcctaca gaaatcttgg cacaacaaca tatggaaagc ttgcaacagc tgtttgatcc
     3901 caatgaagta accatcgcat tgattacggg gtcaacgaaa gcaaaagaac gacgtgagct
     3961 ccttgaaaaa ctagagcaag gggaaattaa cgtcgtgatc ggcacacatg cattgatcca
     4021 agatggagtc gaatttcaga atttaggtct agttattacg gatgagcagc accgttttgg
     4081 cgtgaaccag cggaaagtac ttcgtgaaaa aggctggcga ccagatgtct tatttatgac
     4141 agcgactccg attcctagaa cattagctat tacagcatat ggtgagatgg atgtatctat
     4201 cattgatgaa cttccagctg gacgtatccc gatcgaaaca cgctgggtaa gaacacctca
     4261 attggacagt gtactggatt ggacgaaaaa agaactggca agaggacatc aaatgtatgt
     4321 catctgtccg ctgattgaag aatcagaagc attagatgta aaaaatgcag tggaaatcta
     4381 tgaaaaactt cgtgaactat ttgcaccaaa atatgaagtt ggcttgttac atggtaaaat
     4441 gaagaatcag gaaaaagaag taatcatgga agccttcaaa gacaaccaga tgcagatatt
     4501 agtttcgact actgtaattg aagtaggggt aaacgtgccg aacgcgaccg tcatgctgat
     4561 catggatgcg gatcgatttg gattggcaca gctgcaccaa cttcgtggac gtgttggacg
     4621 cggaagtgat gcttcctact gtatcttagt tgctaatcct aaaaatgaac taggtgtgga
     4681 acggatgaaa atcatgacag agaccaccaa tggttttgta ctaagcgaaa aagatctgga
     4741 attacgtggt cctggtgaag tatttggctt ccgtcaatct ggtctgcctc aatttgcgat
     4801 tgctgacttg gtgacggatg gaaatgtttt ggaggtagcc agagaagagg ctgcagccct
     4861 ttggcaaata aaggattggc agatgcttcc tgagtatcag cctttagcgc aaagcctcca
     4921 aatagaaaat caggataatc gttatttcga ttagcagact gtgctatact ttatactgat
     4981 gacggaaaaa ggagtggaaa caatgaaaat tgccgtagat gcaatgggcg gagatcacgc
     5041 acctcaagca attgtagaag gcgtggcttt agcgcagcaa gattttccag aagtagaatt
     5101 cttactttac ggaaaagaag cagaaatcaa gaaatactta acaaatgaaa aaaacatcac
     5161 gattatccat acagacgaaa aaatcaatag tgatgatgag ccagtaaaag caattcgacg
     5221 aaaaaagact gcatcaatgg tccttgctgc acaagcagtg aaaaacggtg aagcagatgc
     5281 tgtattttca gcagggaata caggcgcgct actagcagca gggttgttca tcgtaggacg
     5341 tatcaaaaat atcgaacgcc ctggcttgat gtctaccttg ccagtagtag ggaaagaagg
     5401 tgccggtttc gatatgctgg atcttggcgc taacgcagaa aacaaaccag aacatttgtt
     5461 gcaatacggt attttaggtt ctttttatgc acgtaaagtc cgaggtatcg gacgtcctcg
     5521 tgtcgcactc ttaaacaacg gaacagaagc aacaaaaggt agtgaagtga cgaaaaaagc
     5581 ctatgaactc cttcagaatg aaacaagctt gaattttatt ggaaacgtag aagctcgtga
     5641 gctactcaat ggtgtggctg atgtagtggt cacagatgga tttacaggca acgctgtttt
     5701 gaaatctatt gaaggaacag caatgaatat gatgaatcta ttaaaatctg ctattctaaa
     5761 tgaagggatc aaaggaaaaa tgggcgctat gcttttaaaa gacggcttac gttctttgaa
     5821 agcagaaatg gattattcca aacatggcgg tgccgttttg tttggcttga aagcaccagt
     5881 cattaaaact catggagcaa caggccccga tgctgttcgc tatacgatcc gccaaatcca
     5941 cacaatgctt gagacagatg tagttggaca attagtcgaa caatttgaaa aaagcgaaga
     6001 atagtatttg gatatctgaa gcgatccgtt ccattgaaaa aaaggtggac attcctgaaa
     6061 aatgccagta aaattagcca tggtgtaggt aattgacaag tgtatgattc ttggaggtga
     6121 aaaagttgac tcgcgaagaa gtatttaaca aagtagcgaa aattatttca aatcattttg
     6181 agatagatac cgataaagtg acggatgaat tgaacatcaa agatgattta aaagctgatt
     6241 caattagtat tatggaattc gttttagagc ttgaagatga attcggtacg gaaatttcag
     6301 atgaagacgc tgaacaaatt gaaacagtcg gtggcgcagt agattatatc agcagccatc
     6361 ttaaataatt tcaaataaaa gcaaagaagg at
//
LOCUS       DACENJ010000141         6386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.145, whole genome shotgun sequence.
ACCESSION   DACENJ010000141 DACENJ010000000
VERSION     DACENJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.145"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..238)
                     /locus_tag="IW748_002004"
     CDS             complement(<1..238)
                     /locus_tag="IW748_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104912.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAK"
     gene            325..1023
                     /locus_tag="IW748_002005"
     CDS             325..1023
                     /locus_tag="IW748_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1104913.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1201..1542
                     /locus_tag="IW748_002006"
     CDS             1201..1542
                     /locus_tag="IW748_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104914.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1535..2206
                     /locus_tag="IW748_002007"
     CDS             1535..2206
                     /locus_tag="IW748_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1104915.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2212..2898
                     /locus_tag="IW748_002008"
     CDS             2212..2898
                     /locus_tag="IW748_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104916.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            2901..3650
                     /locus_tag="IW748_002009"
     CDS             2901..3650
                     /locus_tag="IW748_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1104917.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            3662..3931
                     /locus_tag="IW748_002010"
     CDS             3662..3931
                     /locus_tag="IW748_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104918.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            4094..4393
                     /locus_tag="IW748_002011"
     CDS             4094..4393
                     /locus_tag="IW748_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104919.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            4393..4695
                     /locus_tag="IW748_002012"
     CDS             4393..4695
                     /locus_tag="IW748_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104920.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            4735..5100
                     /locus_tag="IW748_002013"
     CDS             4735..5100
                     /locus_tag="IW748_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104921.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            5727..5915
                     /locus_tag="IW748_002014"
     CDS             5727..5915
                     /locus_tag="IW748_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104922.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            5923..6117
                     /locus_tag="IW748_002015"
     CDS             5923..6117
                     /locus_tag="IW748_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104923.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(6150..6362)
                     /locus_tag="IW748_002016"
     CDS             complement(6150..6362)
                     /locus_tag="IW748_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104924.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
BASE COUNT         2351 a          989 c         1333 g         1713 t
ORIGIN      
        1 ctttagcaat taatttctta aatggagtag ctgaccatct gcggtttaag ttagcggatg
       61 aaagttctaa attaactaac atagctggtt ctgttgaata gttagaaatg attacttttg
      121 tataaatcat atctgcagcg aagtaagaag cattaagttc aacttctagt tgagggcgca
      181 ttccttttat ttttatccct gttaaaaacg tactcaatat aaaacttatt attgacatcc
      241 attgaaaaat tgttaagtga aaaaaattca aaataaacac caccagtttt taactaaatt
      301 ataccaaaaa ggagagaaga aataatgcaa gaattagtaa ttttgaaaaa taaagaagct
      361 gtgactacga gcttacaagt ggcagaaagc ttcgagaaaa aacatcaaca tgttttaaga
      421 gatattgatg cactaaaaaa agatgtgtcc aattttggac agatgtttgt agaaggtaat
      481 gaaccagatt catatggcag aaatcgacga gttttcttca ttagtagaga tggatttttc
      541 ttgctggcta tggggtttac aggaaagaaa gctatctact tcaaacaaaa atacattgaa
      601 gcattcaacg aaatggaaga tgttattcgc aagaatactg ttcctcaaac aattgaagat
      661 atgatgatct atcaattaga agaaatgaaa gatgttaaaa aagatgtttc catgcttaaa
      721 gatactatgc gaattagcgg acaacaagag tttgaaatta agcaaaaagg aaatatgaaa
      781 gttatgggag ttctaggggg aaaagaaagc cgagcttatg aagaaatcag caaaaaagta
      841 ttctcaaaat tttggtctga atttaaacgt accttttcaa tcccaagata tggcgagtta
      901 cctcgtaaga gattcgatga tgctgtttca tttattgaaa tgtggttgcc agaaactgcg
      961 atccgtatgg aaattgatca actgaacaga caacagagac tttttggtga tgacaatgaa
     1021 tagagctgaa gcgctaagaa tagggacggc aattgctaat tgctggtgga aatactataa
     1081 accaatcatc ctaagccaac aacatattga caagcaaaaa gcatggcaac aaataaaaaa
     1141 gtgactccgc cggcaagcaa agagtcacaa agaaaacaca tcataaggag attttagcat
     1201 atggaaaaag aactttccac tctagatcaa tatttgattg atcctgattg gggcaagccg
     1261 aaaattgagg aaacaagtgg tcgaaaaatc agacgaaatc ttttgacgaa tgaagaacta
     1321 gcttgggatc aagatgattt aggcaaccat gtaactattt gggatcatgt ttatcttatc
     1381 catctatcga agcattcgaa taaacctgaa tatatttacg tcatcgaaga tggcttgatt
     1441 gatgcgctag aagagtacga cagagataac ttgattgata tctcttatta cggaccaggt
     1501 aagaaataca ttgctgaaat ggaggcagaa tttgatgagt gaaatcaaag ggacaacgaa
     1561 ctttgaaaaa ctttttagtc gtaagttaaa taaaattctc aagaaaaaag gaaattttga
     1621 ttatttatct tgggctcacg cgtgggagat tatgaaaaag aatgatccac aggcaacggt
     1681 aactattaat gagtacaaac actacagggt tgtttctgga actcatcaag actttcttgt
     1741 tgaggaatat aaaccttttc ttatggatga aactgggact tatgtatctg tctcagtaac
     1801 ggttaaagga cacacggaaa ccgaattatt tcctgtttta gattatcgaa accaaccagt
     1861 tgttaaacca aatgcaatgc aaatcaataa ctcattgaag cgatgctttg tgaaagcatt
     1921 ggctctacac ggactgggat tatatgtatt tcaaggggaa gatattccaa caccacctag
     1981 aatcgataca aagaaattaa acatgctaga gacgattcta gaagctttca atgagcagat
     2041 gggtaaagat atgaccaaaa ccttaatcga atatgttaat gagcagacag ataaattagg
     2101 gctcttagct gataacgttg aaactattga acagttaagc tatgagcaat gtgccttgat
     2161 ggagcgagca atagcagcta agagaaaaga attagataag aagtgatatg agtgtttaaa
     2221 ccattaatcg attcatattc agcggttctg aaaaagttca aaggaaaaga cataagcgca
     2281 accatcaatg aggaagtgaa cattgatcga ctaaagacga tgtatgacgg ctacgatggt
     2341 gatcgagtca ttgaaattcg ttttattgat cctagacgtt tcaccgtaca gcaacgaaac
     2401 ttcatctatg cgctgatagg cgatattttt atcgatacag gcatgccaac ggacttctgg
     2461 aaggaattct tctactttcg ttttgaaggt gtcacagggc gcaaaataag cctgaaagac
     2521 gaatcgaata caactgtgag tgatgccaat gtcttagcaa atatcatctt agatttcatc
     2581 tttgaacatc atattccttt caaagaaggc tatgagattt tacctgcgaa tcaagaatat
     2641 tacttctaca aatgcatcac aaaaagagtc tgctgcatct gtggcaaaac aggagctgac
     2701 atcgatcact ttgacaaagc gctaggaaga cgaaagcgca aagaagttga tcatgcagag
     2761 tacacatttg cagcactctg cagaatccat cacacagaga agcacaaaat aggtgtgatt
     2821 aatttcaaaa ataaatatca aatcaaaggg atcaaattaa accagaaaac aatcaaaaag
     2881 ttaaatattg gagggtaaaa atgacagaac atcgaagtta ttatgcgatt ataccagcca
     2941 acgtaaggta cgacaaaaga cttaaaccaa atactaagtt gttatacgga gagataacgg
     3001 ccttgtgtaa tgaaagaggc ttttgttggg caggcaatga gtactttgca gatttatatg
     3061 gtgtgaataa agagaccata tcgcgatggg taagtgattt gattaagttt ggatacttga
     3121 atcgggaaat catttacaaa gagggtacca atcaaataat caataggtac ctacgaatta
     3181 atcaataccc tattgacgaa aaacgcaata cccctattga cgaaaaagtc aaagataata
     3241 atacatctat taataataca tttaataata caaaagaata tataagagag ttaccgcctt
     3301 cgaaaaaatc gaaggctaag cccgtccgtc ataaatacgg agagtataaa aatgttcttt
     3361 tgtcagatga gcagatggag aaactcaaaa cagaattccc taatgattac caagagcgaa
     3421 tcgaacgact gtcagagtat tgtgaatcat ctggtaagac ttataaaaac tatttggcaa
     3481 ctattcgaag ttgggcaagg aaagaaaaaa gtgaacctaa gaacgcaagc agtggataca
     3541 agcgcacagg aagacgagag aagcttcctg aatgggcaat cgaccaagaa gcctatctca
     3601 agaaaaaagc gctagaacga gctaatagac aatcaaaagc accattctaa gaggtggaaa
     3661 aatgaagatc gattatctag aactaattaa tgaaatagcg aattataaaa agggcgagga
     3721 attagacgtc ctgagagacg tatatgatca actcgaagaa gctggaattg aaggaattaa
     3781 gaatgatcgt tcgagttgga gtaaactcag atactatttc gcactctata tcgatggaac
     3841 gcaattaaga aatttagcat ataccaaatt actattcatt gattgtgtta aaggcttgca
     3901 aaaacatctt aatgaacttg agcaggtgta aacaagatgg acctaaagac atttacagca
     3961 cagatcgaat taatgcatca agaagcttta agacaaagtg tgtcgtacga agacaagtgg
     4021 ctcaacacat ttcatggcgg acgtgagagc gcacttgatc aagtactcaa attactgaaa
     4081 ggagaatgtc gggatggata agaaagcggc aatgcagcga attatcgaat tgacttattc
     4141 agaagattgg caggaagata aaaaaatagt tgcagaagtc caaaagctcg gtaaatcaat
     4201 gtggactgaa aagcccaaac ggagaacgcc gagaaagatt gcaatctggc atggtgatcg
     4261 aattctagta acgggtaccg ctaaacagtt atctgaaatt actggtctga gcaaaaatat
     4321 tatttgggat agagctagga gcttatggat tgattcaaaa ggaagacagt ttaggtatgt
     4381 ggaggagaaa taatggacga actaatcgcg aaagtagagc agtgggcaaa agataaggga
     4441 ttggatcaag cggatttcag caagcaaatg ttaaaaacgg tcgaagaaat cggggaagtt
     4501 gccgcttctc tagctagaaa agatgaacat ggtttaagag acggaattgg cgatgtagta
     4561 gtaaccttga tcatcttggc aatgcaaaat gatatggatt tatacgagtg cttggatttt
     4621 gcttatgacg aaatcaaagg tcggactgga aagatggtca atggtgtttt cgttaagtcc
     4681 agtgacctga aataattcca ttaatggaag aaataactga ataggaggat agacatgatt
     4741 gatgcaaaac aagtgcaaaa acagaaagat ggaatgttga tgtttgaagc atatgttcta
     4801 ccatttctta atcaatttga agttttggaa tgctctgcca gcggagaaga attggagtat
     4861 gttgtaattc gtgaaaccaa agaaaacgtt cagaagctaa acgaatttct ttgcactatt
     4921 aactgttggg atatgatagc tccaggattt ttatgtccag ctatgggaga atttttggaa
     4981 tattgtcgtc ttgaagatgc aggcactttg gatttagctt atctggtata caactaccta
     5041 aacatcaaca cagatcattt gtggtttggc actgccgaaa gaaaatgggt agtccgctaa
     5101 ccaccaatat caatgaaaaa aggcggtttc ctgaaagtgg aattgccgcc attgctgtaa
     5161 ggttctttac gcgactcaag gtcacagaaa tataaatgca aatctctttt gaagtgatcc
     5221 aaagctctcg gaaaatggaa gttttttgtt tcaaagaata aatgagattt gcgtcaagaa
     5281 cctgagagtt atttccaaaa aagcttccaa agaaccgaat gagctaagga catcatgtgt
     5341 aaacacataa tatagctctc ctttcacggc taatcataac atgaaaaata ttcgatgtca
     5401 gtaggaaaaa aggtgaatta tgaaaagaat attaaattat cccggcagta acagattctg
     5461 caattaaact ccgaagctga tgtatcagta tcaactgtag aatattatga gcgtggagaa
     5521 agtcaagcag tcacaaatgt aaaaatcgat aacgtcaacg gtcttatctg tttagatgaa
     5581 ggcgatagta tgaatgaaat tgctgcagac catgaattat cagaaactgc tttaattgat
     5641 caaatcgaat tagctagaca atactttgtt ccatatgcag atacctggtc caagcataag
     5701 aatgacatca tttttccaga ggaacaatga gagcaactga tccagtaatt atccttgagg
     5761 aagccaaatt tatttggact cacgaagaga tagagcaagc acgcttgctc ttttctcaag
     5821 gagttaagcc gagcaaagta gctgaaataa tgggtcaaaa gattcttgat gtcggattgc
     5881 ttttgctcca tctagcagaa aaaaatttga tttgaggtga aaatgatgat tcaacttgca
     5941 ggcatccaaa cagggaaaat ttatttttct ggagaaagca aaagcgaagc caaccaatgg
     6001 ctattgaaaa cttacacgaa caataagaag ctaaggaaaa aatatcctga tttgttgcta
     6061 aaggatgatc agattatgcc ggaaccgatg attttgaccc gtaaggaggg aagttgaaac
     6121 taaaaaacca gccgtgtggg ctggagttgc tatttctttt taataatgag tttttctaaa
     6181 tttaattcat tctcacgagt ttgcgtccaa taatcagaat aatattttaa ttgtttaaga
     6241 gtaggaatgt cgtcttcggt aaaattagaa aataattctg gagaattatc tttttttaat
     6301 tgttctaatc ctgattcttt aaaacgttgt tcaacgagtt ttactaaatt gttgatattc
     6361 atgataaatc acctttttaa atttaa
//
LOCUS       DACENJ010000142         6366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.146, whole genome shotgun sequence.
ACCESSION   DACENJ010000142 DACENJ010000000
VERSION     DACENJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.146"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(46..1176)
                     /locus_tag="IW748_002017"
     CDS             complement(46..1176)
                     /locus_tag="IW748_002017"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1104925.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1825..3027)
                     /locus_tag="IW748_002018"
     CDS             complement(1825..3027)
                     /locus_tag="IW748_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1104926.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3087..3290)
                     /locus_tag="IW748_002019"
     CDS             complement(3087..3290)
                     /locus_tag="IW748_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1104927.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEQLFHIQVAE"
     gene            complement(3432..4664)
                     /locus_tag="IW748_002020"
                     /pseudo
     CDS             complement(3432..4664)
                     /locus_tag="IW748_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4827..5639
                     /locus_tag="IW748_002021"
     CDS             4827..5639
                     /locus_tag="IW748_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1104928.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1898 a         1307 c         1002 g         2159 t
ORIGIN      
        1 aaagccgact ttttgggacg cttttttttg ccaagcctca catttttata gccaaacttc
       61 ttttgcgact tgctgcaata attggatttt cttccattgt tcttcttctg ttaaatggtt
      121 tccttcttct gttgaagcaa acccacattg aggactgata cttagatttt ccaatggtac
      181 aaattgttgt gcttctttta ttcgttcttt tattttctcc gtatcttcca aatctggaaa
      241 cttagaagta actaatccta aaacaacttt actttctttg ttactccaat gtcttaaagg
      301 agtaaaatct cctgctcggt cagaatcgta ttccaaaaag aaaccatcat aatttgttga
      361 aaacaactct tctgcaatgg gttcatatcc accggaaaac agccaagaag aagcgtgatt
      421 cccgcggcag atatgtgttg tgatcgtcaa atcttctggt ttgtcttcca gtgcgttatt
      481 gataatctct gtacaaatat gggccatttc tttcggatca attccttttt gaagaagtgc
      541 ttctctttta gtttgatcag ctagaaacgc ccaatttgta tcatcgaatt gcaggtaacg
      601 gcaacctttt tcataaaaag catgaatcgc tgctttgtaa gtagaggaca agtcctcata
      661 aaaatcagat agatcaggat aaatctcctt gatttttgaa gaaccatcat tagataggat
      721 ctcttgtcgt aaaatcatcg tgggactagg gatcgttgct tttgcaacaa aagtatcatc
      781 tgtttccact acttctttca gaaaagcaaa atcttttaaa aatgggtgat ttggattgaa
      841 agataattta tctactaccc gaatattata agctggtgct gcttttccgt ggaatgtttg
      901 attatatcca gtttctggaa catagccttc gataccattt agttcttcta aaaaatcaag
      961 atgccaaaat ccacgccgaa attcaccatc tgtaattcct ttgatgccga ctgctttttg
     1021 gtcttccact aattttttta tttcctggtc ttcgattgca cgaagttctt cttttgaaag
     1081 taagccttct tgaacttttt ttcttgcttc tttgatcgat tgcgggcgta agaaacttcc
     1141 gacatgatct actgtaaatg gtctattttt gctcatttca ctcactcttt tctttgtttt
     1201 ttgtaaaaaa aaatcctttt actagcaaat actagtaaaa ggacgattat actcgtggta
     1261 ccaccttttt tcagaaaaaa cattctttct ctcagcctga caataaaatc agtggtcttg
     1321 gtaacgaaag actgttcgtt tttgcctacc tttcgacaaa aaatcctcta aggccatttt
     1381 cgacagtcaa cttctagctt tttacaccaa ccaaagcctc tcttgaacca ttttctgtct
     1441 actttccttc tcatcggata ctattagaat aaaattagaa tagcatgttt gaaagaaaag
     1501 tcaataggga ataagaacaa acaaaaacta atttgttatt cttagtaatt tgtttgtgga
     1561 atacaataaa gcttttctaa tatgataatt gttcagtcgt cctttcatct tagatcactt
     1621 ccttaaaatg atcatacccg tttcaaccac ataaaaaagg acttaatttt gaaaaatttc
     1681 ccatcaaaac taagtccttt ttattatagt tatgcaaatt tgcttatttt ctcgctattt
     1741 ccgatacatc ttgaacagga gcaattcgac tgaaaacact ggtgcaaaag catttatttg
     1801 tcttatggta gaaccgtgga aaactcagac tggtaataaa ttgtcatttt ttgaaagaat
     1861 agcgccctct tgaacattat caatcgcact taccacttgt tgatctgact ttgcatataa
     1921 gtgaccgtaa gttcctaaag tgatttgaat attcgcatgg cccaatcgtt cttgaataga
     1981 tagcggttga atattctgct caattagaaa tgcaacatga gaatgtcgta gcgcgtgaag
     2041 cttaatcggt ttgacacccg cttttttagc agcttctttt aaccagttcg caaaagaata
     2101 aggtggcgta aatgttccgt ccaactgagc aacatattca aaatttccaa tacgtttttg
     2161 caagtcatac cactgtctga gttttaagga aagcttcttc cccaacccaa tcgttcgata
     2221 agcacattta gttttgggag tcgaaaagta ccaatcatct tttgtattat aaatcaatgt
     2281 cttctcaatc gttatcgtgt ttttctcaaa atctacattc gaccaagaca aagcttcaag
     2341 ttctccaatc cgaatgccag tataaaagag catttcatac acaatcttcc gatggtgggt
     2401 ttgaatctta tcatccttca attcattcat cactttttga aactcactgt gttcccaaaa
     2461 ctcaactttc ttattctcag aaggaagcga agaaacatct tcaacgggat ttttagcaat
     2521 caccccctct ttaacagccc gatccaatac aatttttagg tgccctcgaa tccgattgat
     2581 ataagatttt gataacgttt cgccattaac ttttttccgt gaattacgtg actctgtctg
     2641 gcttagttca aataaccaat cctcaatatc gtgacttgta atatctcgaa cacgaaaatg
     2701 atagaaaaac tgcatacgct tttcgaaaat gaattgcgca ttctcatatg tttgactttt
     2761 gacctgtctc tcgtaccatt ttacaaacct atccttgtaa aactcttcga aagtcatttg
     2821 attggtagaa cacagttgat taggaattgt cttgttaaaa tcatgtgttt ccatgtactt
     2881 gtcataagca attttggcat ctttttgtga agcataacct cgtttagtct tctgaacctt
     2941 tcttccattt tcatctatac ctagatacgc tcggaagaac catttttttg tctttttatc
     3001 ttggtaaata tttttaattt ttggcatcgt tccattcctc cctgtacttg agaaaagcta
     3061 ctttaatagt accgcgcttg tactgtttat tccgcaactt gaatgtgaaa aagctgttca
     3121 attactcggg atggcaccac accaacttgg cgattattat aaaaatcaat accttcaatc
     3181 cttgcaagat atgccttgct ctctttaatc attcgtgcag cttgactagg tttaaagccc
     3241 atctctatca aaattgcacg atttaccatg aaatcacatt ttgtttccat tctctcacct
     3301 cctgattctt tactcctttt aaaataatcc tctttataat ctattgtttc tcattcgatg
     3361 gttgtgttat actcgtcttg tcgaggcgga gtacgttgca caacttgttg tgtaagcagt
     3421 actttgcttt tttagtagct tgttttctca tttgatatac tttgtaattt tcgttcataa
     3481 tactcaacct cctcctgata atactccggg tctgccttca tattttggag aagtttgatc
     3541 tgtcttggat tcatttctcc ttgttcaata atcaaaggca acaaggaaga atgaaacgtt
     3601 tgatcgacct gtgcaataaa aactaatgat ggtgaaacac catgaattaa ccaactcata
     3661 gttttctcaa aactttgagg aattgtatct aaagaaatgg tgattgcgcc ataattgcta
     3721 ataaaggctg cccatttggc atcaactttt gcacctagat catttggatc acggtcataa
     3781 aaacaaattg ctccattaag taactcaaag aacagtttgg ttaaatcatg ggtttttgaa
     3841 ataatctttg ccaagctttg cgctttctct tttcgatacc gtagttcaaa gcgattcttt
     3901 acttttgcct cttctaaagg aattcctcgt cgcttccgtt gttcatggtc cttctgataa
     3961 aagcaaaaac gacactgact cttctttgag cctaaataca aggtctcacc atttgattca
     4021 ttgtttatga tatccgtacc gccatgagat tcactcactt gaaaagtggt ccacacatgg
     4081 cctagtatta ccttatgctt aagttctgga atgctgagac tccccacaaa atcatctaaa
     4141 gtaaaatcaa tccgtgtaaa gttcccctga taatctaata ccttacgaaa aaactgttgc
     4201 caatttgttt ttcgtgtttt tagccatctt gctaagtgtt tacagccttg acctgaaaac
     4261 tccatcacag ttcctttaac cgcatcgtca atggaatacc gaatcgtgat tacttgattc
     4321 cacacgtatt ggccaatata gccctttggc gcataatcca gttcctcaaa acgttcaatg
     4381 ggcatttcaa taatctcttc aattagcttt aaatgttgat tcgagaaaaa gtgaatggtt
     4441 agataatcaa tcaaaacacg gttaactgga acgacctctt cttgcaaact atccaacaca
     4501 agattcaatt tttcttcact taattgtctc gttccttttt ctactttcga taataactat
     4561 ctcgaaatcc ccactttttc tgctaaatat cgttgtgtcc accccctttc ttctcgagaa
     4621 actcgaatgg cttcaccgag atcatgttga ttaacttggt tcatcgacaa acctccttct
     4681 gctatttact tgtcaaagaa cctattgaac ttattttaag tacattttta ttgtacgcct
     4741 gtatttattt taagtcaata tgttttgact ttttttaagt acacacgtat actaaattta
     4801 agaacttatc ctgaggagga gttttcatgg agatagacaa gcaagcagtc ggcaatcgaa
     4861 ttcggcagat tcgccaagaa ttaaagttga gtatggagaa atttggcaaa ctaattggtg
     4921 atttaccgcg aagtacagtc aataactggg aacgtgggat caaccttcca aaaaccgaaa
     4981 cccttcacca aatcgcagag ataggtcatg tcaccaatga atatcttttg tatggtgatc
     5041 aagagaatca atacatttta gaaatgctcc aaaaaaaggc aggtaagctt gatcctaaga
     5101 ttgaaggtct catagtagac gaaatcaaac acgctgacct tgttagtgaa aaagaaatgg
     5161 atcgaatgat tgaatttttt attacaaatc tcattccacc aactgaacaa gaccagttta
     5221 ctttccaatg tatcgatgaa aaaaagcagc tttatcttgg ttcaactaat tttggtaagg
     5281 aagcccagct ctatttgcaa catgataatc aaaatcatat tctgcacatg atgcccttta
     5341 ccttctccaa ctttagtgtg gatcgcatac tcgtctattt agcaaatcaa aaatcctatt
     5401 cctattttgg gaaacattta cccaaggaac taggtgagaa agccatcctt ctatactcaa
     5461 ttaatcaatt gaccagcgat gtgcgaattg ccccgttggt ctattcagaa gaaaccgctt
     5521 cttatcagta cacagaggac aatcaatatc tactagaaca gcaatatctt tatctaccat
     5581 tcgtgatgga agtcgagaaa gaacgcctgt taaatgcagc gtatccatca tcaaaatgag
     5641 gtgacaaaat tgataagcac gtgacaaaga aaatactgct aggacactcc ccaatgggta
     5701 gttgtcacag tttcctgttc tttgttagcc ccctgttaga accgggggct ttattcctgc
     5761 agtccggcta cgccttcctg caatagacaa aactccagat cgggtaattc gcttgtggcg
     5821 aaacccgacc tggagtttgt ctctgtattt tgaaccctaa aaattaatca aacagactga
     5881 tattctcata atcagcgcga aataagccat taagtacagt tttcctacct atttttgata
     5941 acccgctggt tagttaatat tccttttccg agagagataa atgattggtt aaatctatat
     6001 acatctcaac ctttaaaaat gttttcgaaa tacttcatca gtacatgtca tacaaattac
     6061 cagtgcattc tcaaattcta gttctttaat tgatttcagt tgaggagcag ttacttttgg
     6121 cggaaatttc catttttgtc caaacctctt cccccgatga aatttctttg ttgcaattat
     6181 tgcaatgaaa aatttttgtt tcaaaaatat acataattac ttcctccaat aaaaactatc
     6241 tacctctcaa attatttttc tctaataaga aaaacagaaa aggtaataag atacaacagt
     6301 gatgattcct aataaagaaa agactacaat aatatttttc taacttatcg ccttcaaaga
     6361 aatagt
//
LOCUS       DACENJ010000143         6350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.147, whole genome shotgun sequence.
ACCESSION   DACENJ010000143 DACENJ010000000
VERSION     DACENJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.147"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(39..1334)
                     /locus_tag="IW748_002022"
     CDS             complement(39..1334)
                     /locus_tag="IW748_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1104929.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            complement(1539..3347)
                     /gene="pepF"
                     /locus_tag="IW748_002023"
     CDS             complement(1539..3347)
                     /gene="pepF"
                     /locus_tag="IW748_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1104930.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(3523..4521)
                     /locus_tag="IW748_002024"
     CDS             complement(3523..4521)
                     /locus_tag="IW748_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1104931.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(4747..5400)
                     /locus_tag="IW748_002025"
     CDS             complement(4747..5400)
                     /locus_tag="IW748_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1104932.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(5732..6130)
                     /gene="spxA"
                     /locus_tag="IW748_002026"
     CDS             complement(5732..6130)
                     /gene="spxA"
                     /locus_tag="IW748_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1104933.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1887 a         1294 c         1040 g         2129 t
ORIGIN      
        1 ggacagaaca gaaagaagaa aacctctttc tgttctgttt atttggttaa aaatgttttg
       61 ccatgacgaa tcataattcg tagtttgaaa ttgtaaaagt tgcgaaagcc gtacgcattg
      121 cgttttaata ctttgatgtg gttgtttaag cattccaatg gtccgtttga atagctgtag
      181 tgaagcgcat tttggatctg tggcagaaac tttttaaacg ttcgtaaagt ggttgtatag
      241 gtttctggca gaccgctaat gtctctagtt aaacaatcct caaatagtga ctgatctctt
      301 ttctttaccg cataaagaat gtcttggtat aactgatagc cttggcgtaa gttgggacaa
      361 gcagctaaca gtcgatcggc aatctcactt tcagtcaaat aatctttaaa actggcacgc
      421 caaacccgtt tttcatagtt taactttgct tcttcttttt gtaggagttt ccaatagcgc
      481 ttcaattttt tccctaaccc tcgttgagag tgggaatttc ctttaagtcg taccgtggtt
      541 tctttcactc gatgattcaa gaaagttctg ccaatatgtt ggacgatgtg gaaacgatcg
      601 atgatcaact gagcattcgg gaaaagcttt ttcaccaacg aaacataagg cgcatacata
      661 tcaataacaa tcaattgaac ggcttcacga gtcgctaatg aaaaccgaga aaaatagcgt
      721 tctaaataag gcaagcgtcg attttcgaca atatccaata atgcatggct ttgtccgtcc
      781 atcatgataa aactcattgc actggcaaca cttcgaaccg atttgaattc atcaaagcat
      841 aagacctttg gcaaagtctg tttgaaagga ttcaatgggc ggtagaatcg cttcatgaca
      901 cgatagaccg aagaagtgga aatatttttc tgacgagcga tttccgtcat ggaagtattc
      961 tcttttaagc gagcaatgac agaccaacga actttttgac taatgaaaca gtgtttttca
     1021 gcaacagagg tatccgctac gaatgttctt tggcaagact tacatttaaa tcgttgtttc
     1081 ttcagctcca gataagtgcg atactcagac acatcattca gtaacaaccg aacggttgtc
     1141 gtcccccaac gaatgatcct agaggggacg acacaaagac aatgggaaca ctcttctggc
     1201 ttgtacagaa gagttccttt gtaaatcaag caaatttgcc ctttgtattt ttctttagtt
     1261 aaacaatttt cctcgaaata gatatttagg tctagaatat ttagggtatc tttgataagc
     1321 tgagtatgag acatcttgaa tcctcctatg aattggtagc caaattaatt ctaaaggatt
     1381 tcagggtgtc ttttctatat tttcttatac aaaaaagatg ttggtgaagt tcaccaacat
     1441 caaaaagtat agagccttaa aaaaatacgt cgactccctt acaagagagc cgacgtattt
     1501 ttttcgtttc tattcgagaa aatcaaaaat agaaagcctt ataaacgatc gatcaattct
     1561 tctaattcat ttagacgttg ttcaaacatc gacattgcat cttcaatata tgcagcctgt
     1621 gtcatatcaa ctcctgcttt tttcattact tccacaggat aatcgctgtt acctgctttt
     1681 aggtaagcca agtagttctc tagtgcctct ggttcctggt ttagtatttt cttcgccagt
     1741 gctgaagcag cagaaaagcc agtcgagtat tggaaaacat agtaattata ataaaaatgc
     1801 ggtatccgtg accattcgaa tttaatttct gggtcttctt cgactgctgg accataatat
     1861 tttgcattca atttaccata actatcactt aggtattcac tagtcaatgg cacacctttt
     1921 tcatcttccg tatgcatgaa atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt
     1981 cctttaaacc catccaagta atggttgagt acataagccc gtacacgagg gtctttttct
     2041 gtttccaata aatactccgt taggatattt tcattcgttg tcgaagcaat ttctgctaaa
     2101 aagatggagt agtcgccgta cacataaggc tggttcgaac gagtgaaata actatgaaca
     2161 ctatgtccca tttcgtggac aagcgtaaat agctgatcca gcgtatcatg ccaattcaat
     2221 aaaatatatg gatttgtgtc atagcttccc gaagaataag caccgcttcg tttcccttta
     2281 ttttcgacaa catcgatcca acgttcagag aatgcttttt ctacgatggc catgtattct
     2341 tcacccattg gtttcagtgc ttctaaagct ttttcttttg cttcttcgta cgtaaaggta
     2401 attggagctt cccctaagac cggtgtataa agatcataca tgtgcagttt ttccacttct
     2461 aataaacgtt tccgtaattc catgtatcga tgtaacaaag gcaaatgctt gtttaccacg
     2521 tctaccaaag tatcgtatac actttcagga atatgattat tgctcaaaga cgcttctctg
     2581 gcagagctgt aattacggac tttcgcttta aaattatgtc cttttatatg tgtgcctaaa
     2641 gtagaagcaa atgtatttct aaattgttcg taaacactgt acaacccctt aaatgctgct
     2701 tcgcgcaccc ttcgatccgt gctttctagc aactgaccat acacgccatg agataattgg
     2761 actatttcac cattttctcc ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca
     2821 gcaaatgtat cacttgaagc atcaaagatt tcacctgctc cagcaagaag agattcctgc
     2881 tcagcagata agacatgggc tcgattatct actatttgct ggatataatg gcgatagact
     2941 tccaattttg gttcttcttt gaaatattgc caaatctggt catctgacaa ttgcaatatt
     3001 tctggttcaa accaggaaac ggcttcacta actttcgaaa acagactgct tgctcttgca
     3061 taaagcgctt ggtaatctgt atttccagtg tcttgatcgt ttttaaggtg cgcatataca
     3121 taaatgactt cagtttggcg ataaaccctc aatacgaatt caatcgcatt taaaaattga
     3181 gaagcgcctt gatcaagtgt tcctttgtgt ttttcagatt gttttaactc ttctgataat
     3241 tccaaatatt tctcatcaaa ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt
     3301 aaattttcag gcaattcttc tcgttttggc aactgcttta cttccatgaa atccactcct
     3361 tttcttccct tagctctatc tatcaaaata gatcatagtc taacatccac atgtcacagc
     3421 atctaacttt tctactcatt catgtgtctg cttccatcaa ttttagcata actaaatctt
     3481 gttttacata gaaaaccgat tcctagccta tttttatact tactattttt tcaaagaaga
     3541 caagtgtatg cattcgttaa aaaactgctg atagaccatc tcttcatcaa tcataggaaa
     3601 aagccattcc cttttatgtt cttttatttg gcaaagccat gagttgaaat caggaacttt
     3661 ctggttttcc aaggcttctt cgtacaacat tcgataaaga aaaacctgtt cttgtaaata
     3721 aaagaaaaac cgactttttt gatagatcca cgatgataga tgcaaaagat gtttatgacg
     3781 caagtagcat tgttcttgta tggatactgt attcttttgc ttgtgaataa gttggcgtgt
     3841 cagccatttc ttcccatctt cactcacttt ttctattttt ttcatttggt tcattttaat
     3901 ttcgttacta gtgaatattt ctttaaggtc cctactagaa aaagtccagc agctactgca
     3961 aaaggaaata tgccctgata cagtctctgt aatatgatga tacaaataaa gcttttgttt
     4021 cttgagatca gctttccagc aatgcactcc tctttttcta ttatagtagc agcaactttt
     4081 ttctatcaat gagaattgcg attgtccaag aaaagaatta ccaagtatcc accatacttg
     4141 gtaactgtta ttcaaataat tttgggttct ttcaataaat ctctggtgag acagtctaga
     4201 acattggatt tctattgcta gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg
     4261 caaataggct tccaaaaata ctggttctac tgactgtccc agccaatcca tgaagcattc
     4321 ctttaactgt aagtgctctt ttgattctct ttcactaaag accgaacagt tatctgattt
     4381 ttgatgggca aaatgagcac attttttctt tccctttttc aaaatcactc tttttcgaca
     4441 gccacgacaa taatatttct ttcctttttc tgcatgttta gcaaaacagc acttattata
     4501 ttcattcatt gcaagcagca tttttctccc tctgaactta tttacgtcta agaatttatt
     4561 tacacgaaaa aagagggcgt gaaaaagcta ttctgcatca agtaataaga acagctaaac
     4621 aaaaatagtt cccaatattt ttcgtttggt tgggcttgtt accgcagatg caagcttttg
     4681 aacacattta tacgaaaaaa agactgcgac acaatttttg tcacagtctc ttactataat
     4741 gctgatttat acaaaataaa atttcgttcg ctcgatagca ttacgcgcca tcagacattg
     4801 tccatattca ttcaatgttt ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg
     4861 agcaatctga ttctctacat ccgcttggtt gagattttcc atccagaagt gaacgactag
     4921 gtaatagcgc tcttcgaata aatacaaatc agtccaagca gactctagtt cgacatctgt
     4981 tgcaagttgg atcacttgtt caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc
     5041 ttctagggcg cttccagata attcttcctc cggatcgctg ccaataatct gctgacgaag
     5101 aaagtcactc acatcttctg aatcaacagt agaatcttct gattgagaaa tttcttcatc
     5161 cggaagattt ttactgataa acagttctaa tccgtctcct tttggcaaaa cttggaaagt
     5221 gacagcttcg cttcctcgga actcctcttc gatatcaact tcttccaaaa tactataaaa
     5281 gaaattttcg atttctttgt gattgccaag taaatccaaa aatgaaacgc cccgttcctc
     5341 taaatcttca tgaccaatta gtacacggat cgtattttca ttaatatgtt ccatttccat
     5401 tttgctacac ctcactttgc cacaatgttt aatatagatt cattgtagcg aaatcagctt
     5461 aaatgtaaag aaaaaaaatc tcgctgttaa gcgaatttaa taaaaccagc ggatttttca
     5521 acctttcaag ctatcttatt taactataac atttttggca gacgaatact atatagccga
     5581 cttttcagct aattttccct ctctattttt cgtgcaatca tcatcttcta ctaccttttt
     5641 atacatctaa tcaagaatac tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt
     5701 aatagtcttt tccaagtttc gtattatcct tctataatcc agccatcaat tgtgcttgac
     5761 gtaattctaa ttgtcgtaca tcgcgcggta ggaaccgacg aatttcatct tcattaaaac
     5821 caacttgcag acgtttatca tcaatcatga ttggacggcg aagcaatcca ggattgtttt
     5881 gcacaagttc caataattct tgtaacggca gatcatctaa atccatgttc agtttttgga
     5941 atactttgga acgtgtcgag atgatttctt ctgttccgtc ttctgtcatt tgtaaaatcg
     6001 cttttaattc agaactgttc aatggttctg aaaaaatatt acgttcaaca aatggaatct
     6061 gatgttcttg caaccacgca cgagcctttc tgcaggaagt acaacttggg gaagtatata
     6121 atgttaacaa cgtgcaccac tccttttcgt gataagaatc acttttttgt aacagaagat
     6181 cataataatc tcccatctgt ttctattata cataatcaac ttttaaatag ctaccttttt
     6241 ttcataattt ttttaaaaaa atgaaagtta tctaaggtta atatacagta aaaccagcat
     6301 tctcgctttt tttatcattt aaaaattaga gttttcaaat ctaattcaaa
//
LOCUS       DACENJ010000144         6288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.148, whole genome shotgun sequence.
ACCESSION   DACENJ010000144 DACENJ010000000
VERSION     DACENJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.148"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(239..916)
                     /gene="nth"
                     /locus_tag="IW748_002027"
     CDS             complement(239..916)
                     /gene="nth"
                     /locus_tag="IW748_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1104934.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(923..1621)
                     /locus_tag="IW748_002028"
     CDS             complement(923..1621)
                     /locus_tag="IW748_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1104935.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1889..3073
                     /locus_tag="IW748_002029"
     CDS             1889..3073
                     /locus_tag="IW748_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1104936.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3079..3522)
                     /locus_tag="IW748_002030"
     CDS             complement(3079..3522)
                     /locus_tag="IW748_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1104937.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3748..4035)
                     /locus_tag="IW748_002031"
     CDS             complement(3748..4035)
                     /locus_tag="IW748_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104938.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4206..5558)
                     /locus_tag="IW748_002032"
     CDS             complement(4206..5558)
                     /locus_tag="IW748_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1104939.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5831..6277)
                     /locus_tag="IW748_002033"
     CDS             complement(5831..6277)
                     /locus_tag="IW748_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1104940.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1691 a         1257 c         1052 g         2288 t
ORIGIN      
        1 atcctttgga atactggact tattttatag gtatattgga aggcattatt ttctacatcg
       61 ggtattttcc caataaaaat aacaactttt atttttatcg caataaattg taataagtta
      121 ccatcctagc tactgagtat gtacatttac tttttcatgc aaaaaagata aacgaaacag
      181 cttttctcca taataacaag ataattcata catttatttg caattaaaga aaccacgtct
      241 acaggcgtgg tttctttttt agcgcctttt cttttagacg catccgattt ttcccatctt
      301 gacatattga tagcaaagga catacctcgc acttgggatt tcttgccgtg caatgatatc
      361 taccaaaaaa aatcagtgta tgatgtgtct ttacccataa ttcttgtggt actttccgca
      421 tcagtgtttc ttctacttcc atgaccgtag catctagttt gcagatcctc aggcgttttg
      481 agacacgttc cacgtgggta tctactgcga ttgctggtat cccgaatgca tcccccaata
      541 cgacatttgc tgtctttctg cctacaccag gaagagacat caattcttct cttgacgttg
      601 gcacttgccc atcgaaacgt tcaatcagct gctgtgcaca cgctttgatg ttttttgctt
      661 tatttcgata caaaccaatc gtttttattt taagaatgat ttcatcgatt gaagcttctg
      721 ctaacgcatc gggtgtcgga aaagaggcaa acaaatctgg cgttgcttta ttcactgata
      781 catctgtcgc ctgggcactt aagatcactg caatcaataa ctcaaatgga ttattgtgct
      841 tgagttctcc atgtgcttcg ggaaacattc catacatcgt ttctagagct tccattgttt
      901 tttgtttgct taacatattc cgctactcct ccaaccaatt tttcagtgag actttcggca
      961 attcttcttg ttgagctgac tgttctactt ctctttgaag aagctgctgt tttcttcgtt
     1021 tttggtcttc ttccacttgt tgttttgtac gtagattttt tctttcccac gatagcaaaa
     1081 tgcggtcaac gtaattaaaa ctataagcct gatttaatac ggcttcccgc agtgctagct
     1141 tcaaaatttc cggctgataa tggtcttctt ctagccattg tccgatccgc tggaactcga
     1201 tcgaagaaag cgggcgtcca aattcttgtt caaataattg atacactgac tggatttctt
     1261 tttcttgtgt tttttcttcg tatttttttt cttgtgtttt taaatactca cctaatagat
     1321 catagatggg gtaaagatta tatcgatctg cttttttacc gttgacaatc gtcgtttcta
     1381 tcttgataaa tccatttgtt acgagacgat ccagtatttg aaaaatctga tcttgtttga
     1441 ttcccatatc catagcaatc atctgcaaat cagggaaagt atctccttca agctgtgcca
     1501 tatgcaactg tagaacaaat aaaaattctt cagattgtag accgatccta tgataatttt
     1561 taagtaaaag attagatatg actgtttgtc cagcgcttaa gtaattttct aaattcaaca
     1621 tgatttctcc tccgctcaat cacagtatac gtaaaaaaaa catgggaagc aagaacaggt
     1681 ttcttttcta tttacttcac tgatcggtca gatttacaat gtcgtctact tgatttttgt
     1741 ttgttcatgt ttttgatgcg tatcttcact tttcggaata aataaattaa gtagatttcg
     1801 ctgttcctta gaatgttgac aaggaaacaa aattactata cactattttt gtttccaagt
     1861 taacattatt gcataaagga gtttttacat gtacagtttc acaaataaac attctaaagt
     1921 ttttggatac gtctctgtct tattcactgg attaggattt actatcgtca gtcctgtact
     1981 tccgttttta acgctgcctt attctcactc catacatcag caagctttct atatcacttt
     2041 gctcatgtct gtttatgctc tagctgcctt cttatctgcc cccattctcg gtagcttaag
     2101 tgatcatttt gggcgtcatc cgatccttat tatcagttta ttaggatcaa gtatcggata
     2161 tttgcttttt ggtttaggaa attcgatatg gatgttgttc cttggtcgta tcattgaagg
     2221 attgacggca ggagaaatct ccaccttata tgcttacttt gccgacatca cggaacctaa
     2281 tgaaagaacc aaagtttttg gatggatggg tgctttagtc ggtataggaa caacgctggg
     2341 tcctattata gggggtctat tagctgagtt ggggaattct gttcctatat ttattggagc
     2401 actctttact ttcttaaatg ccgtttatgg ctacacattt atgctagaaa gccttccaat
     2461 aaaaaaacgt tcagtagatt taagtttctc ccatgtaaga ccttttcacc aattacaaca
     2521 attattcaag atttcatccg ttattccttt attgacagca ggttttgctg tttggttggc
     2581 agctggctct cttcaatcta tcttttctca attttcgatt gacgcctttc agtggaaggc
     2641 tgggttggtt ggtttaagct tctctttgat tggaatcttg gatactgtct cgcaattgtt
     2701 cattatgcct cgattgctca aaaaattttc agaacagcag atcacaagaa tagggatgtt
     2761 cagtgaaatc ctcgcctatc tattcatcac attatctgga attcttctac tgccgatttt
     2821 gtttttattt gggattatct gttatggttt cggtgattct atttttactc ctgtattcaa
     2881 tggccaacta tccaattctg tctcagataa ccagcaaggt cttgtaatgg gcggtaccca
     2941 aagtatacaa tctctatcgc gcgtcatcgg cccgctaatc gcaggacagc tttatgctgc
     3001 agcaccattt cttccgacgg tattcggatt tttgcttatg atttttgcct attatcacta
     3061 caataaaaaa tagtagcttc atttgctact tcgtataaat tctctgtatc gaccgataaa
     3121 acgaaatact gtgtcaaatt cttcttttga catattttca aatacagtag catctcgctg
     3181 tacccatttt tgatggagtt gctcgtgtct attccttatt ttttgtccga ttggcgtcaa
     3241 ttgaaaatag atttcttttt tattctcttc gctttgataa gtgtttatga tccctctagc
     3301 tatcagtttt tttgttagct tactgatggc tcctctagtc atgtaactag cattcgctaa
     3361 ttttgtcacg ttaggatcag ggattttccc gatatgttca atcacttcta gttcatttag
     3421 agtaaatcct gcaaattctt tttccatttc tggtttatta cgatgaataa tctcattagc
     3481 taaatccaga tattcttcta ttagcagttg tcttttatcc atttctacac cttctttttc
     3541 tttcattata ttgttttttt gtttcctatt caacaaagat aaaaatattt tgtaggaaaa
     3601 cccaatctga aaccaagaaa aaaaggggtt gacaaagctt gtcataaccc ctttttctga
     3661 ttaaaagata gtaaagagac agtcatcggt aattgtgatg attttttgtc atcacttaag
     3721 gatgagcggc cttttcaaaa actgtcttta aagatcatca tcagtttcta gcacatatgg
     3781 ctgaccttca tatgtgtact ctgtattcaa tgtgccgtca ttttcatctg agtagatgcg
     3841 tctactgaaa ggaaaacgat gagaaacatt atctttggcg tcatttgctg gcatcgtgta
     3901 atattcctct tgcgtttcat tcaactgaga gagtacttga tctgccaact catcaatatt
     3961 gatcgttaat ttcttcgaag cacttttata atcttctaaa aatgcatctt gttggacaaa
     4021 atattcttta ttcatcttct ttctcacctt tcttttcaaa atcaaataaa gctgcttgtt
     4081 cttttatgta acagctttat ctttgctgct tattgtgaca aaaaattcga agaaagacaa
     4141 ctaattgtac ttttcacaaa aaaagacaga gggtcgtgac aaaattgtgt cacaaccctt
     4201 tttctttatt gtgcagtttc gatttcgcct agcgctgctt gtgcaagtag gttcaagtaa
     4261 ttccatgggc ggtcaaaatg tggttggaag aagaaatctg aaagtgcgag atcttctaca
     4321 gtcattttat tttgaacagc taaagacatg gtatttgctg attgtgtaat gtcgtatttg
     4381 gacatgaact gagcacctac gatacgattc gttccttttt catagactag ctccatcatt
     4441 actttttcag tagttggcat gaactctgga cgataattat cttcaaatac agtcgcttgg
     4501 acttctagat cgttcaaagt tgcgctttct ttggtcacac ctgtcgaacc gatcgtccag
     4561 ccaaataggt aaagtcctga agttccttga gtaccacgat acgccatttt atgatctgtc
     4621 aagttatttc ccacaagcaa accttgacga acggcatttg ttgccaaagg aatatagttt
     4681 tgcgtgccac ttggattgta gttgactact gcagagtctc cagctgcaaa aatgtctgga
     4741 ttacttgtct gcatgtattc attcactttg attgccccgt ttggcagtgt atctactttt
     4801 ccttcgatca gttttgtatt tggacggaag cccacacata aaatgaccat atctgtatca
     4861 aatgtatctg ttggtgtagc tacttgtttc acttttcctt cttcatctgt gataaattca
     4921 gtaacatttt cgcctagcgc aagagtaaca ccgcggtctg tcaattcttt ttctaagata
     4981 tctgtgaatg gtttatctag atatttattc aagatgcgat ctaatccatc aatcaatgtc
     5041 acttgttttc cagattcgac aaaagcttct actaattcaa taccgatata tccaccacca
     5101 acaattacta cacgttttgc ttctttcgct tgtgcaataa tttcatttgc ttgattgtag
     5161 tttttgcata ggagtacatt tttcgcatcg attccactaa tcggtgggat aataggccat
     5221 gaaccagttg tcatcactaa tttatcataa gaaaccgttt ctgtttctcc tgttttcaga
     5281 tcttttgccg ttactgtttt attttctgta tcgacgtctg ttacatcgtg ttccatttta
     5341 actgttgctc ctaatgaaga taattcttca ggatttgagt agaacaagcc tgctgggtcc
     5401 tttactactc cgccgacata aagtgcgata ccgcaagata agaaagaaat attgtcattt
     5461 ctttcatata ctgtcacttc tgcttctgga tgatttttta aaatactttt tacagctgca
     5521 gtacctgcat gagtacagcc aattactact actttcattt atggtccctc cgattaacat
     5581 tttgtccttc gacaaaacta ctataacaga aataaaacgg atacaacagt ttaatgcttg
     5641 tgaattcaca aaggtttgtt tgtagtttca ttccttttca agcataagaa cccaatatct
     5701 acaagattct tattttaata atattcactt ttatatctaa gattgtgaat aaaagacaaa
     5761 aaaataaaaa agctagcgtt ttttttacac agctagcttt ttttaggtat aacacttgta
     5821 gaaactgttt ttacatttgc gccttttttg ctgactcttt acaatccgga cagagtccat
     5881 acaattccaa ccgatgttca ttaatctcaa aatcagtcag tctgcttgca gccatttcta
     5941 tatcttcaag ccctgggtaa tgaaaatcaa caatcttacc gcagttttca caaattacat
     6001 gataatgacg ctttgaactg aaatcaaatc ggctagaagc atcgccgtac ttcatttctt
     6061 gaacaaatcc aatcgaagta aataagcgaa ggttattgta aacagtcgca acactcatat
     6121 ttggaaaccg atgttcaagt gctttataaa tctcatcagc cgttggatgg gaatgatgtt
     6181 caatcaaata ttctaaaacc gcgtaccgtt gaggtgtgat tcgaatgttc tcttttttta
     6241 acttttcgat aacctcttcg actaacttat tttccatgta aacacccc
//
LOCUS       DACENJ010000145         6274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.149, whole genome shotgun sequence.
ACCESSION   DACENJ010000145 DACENJ010000000
VERSION     DACENJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.149"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            199..1830
                     /locus_tag="IW748_002034"
     CDS             199..1830
                     /locus_tag="IW748_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1104941.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSNIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1833..2912
                     /gene="uxuA"
                     /locus_tag="IW748_002035"
     CDS             1833..2912
                     /gene="uxuA"
                     /locus_tag="IW748_002035"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1104942.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLQTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2986..4413
                     /gene="uxaC"
                     /locus_tag="IW748_002036"
     CDS             2986..4413
                     /gene="uxaC"
                     /locus_tag="IW748_002036"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1104943.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLGYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4506..5153
                     /gene="eda"
                     /locus_tag="IW748_002037"
     CDS             4506..5153
                     /gene="eda"
                     /locus_tag="IW748_002037"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1104944.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5273..>6274
                     /locus_tag="IW748_002038"
     CDS             5273..>6274
                     /locus_tag="IW748_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1104945.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLIIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQFKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     "
BASE COUNT         2163 a          834 c         1285 g         1992 t
ORIGIN      
        1 gaaaaaaaga aaaaaataag tttcgataaa atcagtccaa aaagaaggag tattttattt
       61 ttctgaataa aaaatacaat aattctgata tggtagaaaa aaagcaagtt tattccattt
      121 ttatattgac aacggttaca acgggtttta aaatataaaa tgtatcaaaa ctaacatatt
      181 agtttaagga ggttaattat ggtttcgatt aaaaattggc aaaattctat cgaagaattc
      241 aatgaaatgc agattctact gccgtgtttt gatgtcaaag aagttaagca agaaggccag
      301 caacatccag tatggcttca ttttggtgga ggaaatcttt atcgaggatt tcatgcagaa
      361 attgctcaat gcttagcaga tcaaaatgaa ttagaaacag gagtagttgt gtgtgaaaca
      421 tttgatgaag aagtagtgga aaaagcttat catgactaca acaatgacat tttggaaatt
      481 gttatgagtg gagatggtga actcaaaaaa aggcttttgg cagtaacggc tgatagttta
      541 tattgcaatc cgaagaatat agcggactat caaagagtgg ttaaatattt tgaagatcct
      601 aaattacagt ttacaacgtt tactattact gaaaaaggtt acagtttaaa agattctaaa
      661 gggcaattct tttcggtagt caaagctgat atcaaatctg gacctagcaa tcctcaacat
      721 acgatgagta ttgtaacatc cttgttatat agcagattta atgctggaga gttgccgatt
      781 gcgatggttt ctactgataa tttctcacag aatggtcaac gttttcaaga tagtattttt
      841 acgattgcag aagcttggca aaaagaagga tatgtttctg aaaaatttat tgcttattta
      901 aaggataaag aaaaagtgac ttttccttgg agtatgattg atcgaattac gccaaatcct
      961 tcagagaatg taaaggaaga tctattgatg atggggctcg aggatttaga tatcattcac
     1021 acaaaaaaac ataccaatat tgctgctttc actaatactg aggtagttca ttatttagtt
     1081 attgaagata gttttccaaa tggccggcct aaattggaaa aggctggtgt gattatgaca
     1141 gatagagaga cggttgataa agcagacatc atgaaggtta cgacaagctt gaatccactt
     1201 catactgctt tggcagtaac aggttgtctg ctaggatatc aatcaatttc agcagagatg
     1261 caggatgatg atttggttgg tttaatcaaa ggaataggtt atttagaagg tttaccagta
     1321 gttgaaaatc caggcattat tgatccaaaa caatttattg atgaagtaat taataaacgt
     1381 ttgcctaatc cctatattcc agatacacca caacgaattg caacagatac ttctcaaaaa
     1441 gtagcgattc gctatggtga aacaattaaa aagtatgtcg aaaaagacgg aaatgcagaa
     1501 aatctaacat ttattcctat cgctattgct gcttggttac gttatttact ggcagtcgat
     1561 gatcaagggg aaaattttgt gccaagtcct gatccgttat tggtagagtt gcaggataaa
     1621 ttatcagtat tgaaattagg tgaacaagat ccttcaaata ttcattgtgc ggtacaggaa
     1681 attttagaaa ataaagaaat ttttggttta gatttatatc aagttggggt aggaaagaaa
     1741 actgaaaaaa tcttttgcga aatgctagta ggtcctcaag cagttcgtaa aacaatcaat
     1801 aaatatataa cagaagttgg aggaaagtaa ctatggaaat gacttttaga tggtatgggg
     1861 caaatgaaga aaaaatctca ctacaagaaa ttcgtcagat cccagcaatg aaaggaattg
     1921 ttggaacatt aatggatata ccagccggag aggtttggcc gagagaacga attaaatctt
     1981 taaaagatga agtcgaagcc aatggactta ccttgaaagt tattgagagt gttaatgttc
     2041 atgatgatat caaaattggt ttacaaacac gagatcaata cattgagaat tataaacaga
     2101 cgattcgcaa tttggcagaa tttgggattg aggttatctg ttataatttt atgccagtat
     2161 ttgattgggt aaaatcggat ttggattatc gtttagatga tgggtcttct acgttagcct
     2221 atatttcaaa aaatatccct actgatccgg aggaaattat tagaaaagta gaaaattcta
     2281 ctaataattt tgaattgccg ggttgggaac ctcatcggtt atcacaaatt aagcaattat
     2341 tagaagccta taaagaagtt gatgaagaga aattacgaga gaactttgca tattttctca
     2401 aagcaattat cccgacatgt gaagaagttg ggattaaaat ggcagttcac ccagatgatc
     2461 caccatattc cattttcgga ttacctcgta ttgttaaaaa tcgtgacgat ttagattggt
     2521 tgtgtaatgt agtggattct ccttctaatg gtattacatt atgtactgga agtattgcag
     2581 aacgaccaga taatgatgtc tatgaaattc tatctgaatt ttgtcaacgg gatcggattc
     2641 cttttgcaca tgttcgtaat atcaaatatt taagtgacaa tggactcgat ttttacgaag
     2701 cgccgcataa atcagaatat ggttctctag atatgtataa gatattaaaa acaatgtacg
     2761 ataatggatt taatggctat attcgtccag atcatggtcg gatgatttgg ggagaaaccg
     2821 gtagacctgg ttatggatta tatgaccgag ctctaggtgc agtttattta aatggtattt
     2881 gggaagcgct tgaaaagggg gcgagtaaat aattatataa tggagtttat ttagaggaaa
     2941 aattttctct tctaaaaact aacggaagga aaggagtgtt taaatatgtt tttagataag
     3001 aactttttat tagagaatga aatagctaaa agactttttc ataaatttgc aaaacaccaa
     3061 ccaattatcg actatcattg tcatttagac ccagcatatt tgctagaaaa taagcaatac
     3121 aagaatttga ctcaggtatg gttaaatgat aaaggagctg gagatcatta taaatggcga
     3181 ttgatgcgag ctaatggtat agaagaagag ttgatcagtg gtccaggtga tgattatgaa
     3241 aaatttttag cttttgtaaa aacagtggag aaggcgccag gtaatcctat ttttgaatgg
     3301 tctcatttgg aattacaacg ttactttgat attgatttga caatttctga gaagaatgca
     3361 ccagaaattt ggcgacgtgc taacgaaaaa atccaaacgg attcctatcg cccgaaagaa
     3421 ttgttggaga tgatggatgt gaaagccatc tgtacaacgg atgatcctac agatgacttg
     3481 gggtatcata ctgagctttc taaacaggat aattctttca aggtattacc tactttccgt
     3541 ccagataagg tctggagtat caaatcatca ggcttttcga actatatttt aaaacttagt
     3601 caagcagcgg ctataccaat caaagacttt aatgggttga agcaagcatt gagccaacga
     3661 gtggatcatt ttcatcaaat tggctgtcga ttagcagatc acggtttgaa tacttatttt
     3721 tatgaaatag cagatgaaaa aatactcgac aatctgttaa taaaagcttt agcaggaaaa
     3781 acggatttcc atttatcaga aattgcacaa tttgttacag gtattcaatt acatttgatg
     3841 tctgagtata caagatatgg ttggacgatg caaatgcatt taaatgcttt aagaaataat
     3901 tcaagtatat taaaggaaca aattggagta gatgtcggtg gtgattcgat gggggatcaa
     3961 gctagtttga cacaaaatgt tgtagcattg ttcgataccg ctgaaaagag tgggtgctta
     4021 ccaaaaacaa ttttatattc attaaatcca actgattggc ttccactagc aacagcaatg
     4081 caaagcttcc aagggggtat gattcaaaaa atccaattgg gatgtgcttg gtggttcaat
     4141 gatacagctg aaggaatgcg tcaacagttg actacgatgg cgcaacaaag tttgttgtcg
     4201 aattttacag gtatgttgac tgattctcga agttttctaa gctatcctcg ccatgagtat
     4261 tttcgtcgag tattatgtca attactaagc gagtgggtta aagcaggccg aattccagat
     4321 gatgaaatgt atcttagtca gatagtggag aatatttgtt atcgtaatgc gtataattat
     4381 tttaattttt ttgaaatcgg aagtagaaaa taatacttga aataaaagct taaatttatt
     4441 ctttttaagt tagtacagct agacaaagta tgtatccata aaagaaggag atagaggaga
     4501 tagatatgaa aaaagtagaa attttaagta agttagaagc ggctggagtg attgcggttg
     4561 tacgtgggaa aaataaggat gaggccatta aagcaataaa ttcaattatt gcgggtggaa
     4621 ttaaagggat tgaactaact tttacagttc ctaatgctga agaaataatc aaagaaattg
     4681 ctaaaaaata tgctgatcaa aaagatgtaa taatcggagc cggtacggtt ttagatgcgt
     4741 ttactgctcg tatcgcaatt atggctgggg cagaatatat cgttagtcca tcttttgatc
     4801 gacaaacagc tgagatttgt aatttatatc aaattcctta tttaccaggt tgtatgacaa
     4861 ttactgagat aaaggaggca cttactgctg gcgtagatat cattaaacta ttcccaggtt
     4921 ctgcttttgg tccatccatt gtaaaagcct atcaagctcc attaccacac gtaaatataa
     4981 tgcctactgg cggagtaagt ctagagaact tagaagattg gtttgaagta ggagttgttg
     5041 cagttggtgt aggtgggaat ttattggctt ctgttgaaaa aggtgatttt gaagaagtaa
     5101 gtcgagtagc tagtaaatat atagataagt tttatgagat aaagaattat taggagaaaa
     5161 aacaaataat actctgaaat gtaaaaaata aatactgact ctttacgctt cagagtatag
     5221 tacgtacttt cataagaatg aaaacgctta acaaaagaag ggagagaatt gtatggaaag
     5281 aaaatttggt ataagagaca aaataggata tatgtttgga gattttggaa acgattttac
     5341 tttcattttt gctagttctt ttttaatgat cttttataca aaagtattgg ggataagcgg
     5401 agctgccgtt ggaacattat ttttagtagc aagatttatc gatgcagtta ctgatgtaac
     5461 tatggggcgt attgttgata gatcgaaagc aactaagcag ggtaagttta aaccgtggat
     5521 tttaagaatg agtggtccag tcgctttagc aagtttttta atgtatcaaa cttctatgat
     5581 ttcagcttct atgagcgtta aggtagtata catgtatgtt acgtatatat tatgggggag
     5641 tattttttac acatctataa atattcctta tggatcaatg gcatcagcta ttacttctat
     5701 tacagaagaa agaacaatgc tatcgacatt ccgaaatgtt ggagctactt tagcaagttt
     5761 gatcattggt gtattaacac cattatttat ttatacaaga gatgattcag gcgctcaaat
     5821 agttcgtggt ggatcaactt ttacaattgt tgcgggaata ttttcgatat gtgcacttat
     5881 ttgttattta atatgttata aactaactac tgaacgagta agaattgacc aatctcaatt
     5941 caaagtttca ttgttaaaat ctttaaaagg gttagttaaa aatagggcat tactttctat
     6001 aattggtgcg gctattttct tactccttgc tcaattatta attatgagta tgaataatta
     6061 tgtatttcct gattactata atgatgcaca gggaatagtt ttaatgaatt ttatcaatcc
     6121 tattttggtt ttaatagttg tttcaccttt aactttgttt ttaagtaaaa aatatggaaa
     6181 aaaagaactg gcatcagtag gaatgttctt ttcagcaata gtatatttga tcttattttt
     6241 gataaaaact agtaatatgt atgtattctt aatt
//
LOCUS       DACENJ010000146         6114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.150, whole genome shotgun sequence.
ACCESSION   DACENJ010000146 DACENJ010000000
VERSION     DACENJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.150"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            293..1480
                     /locus_tag="IW748_002039"
     CDS             293..1480
                     /locus_tag="IW748_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1104946.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            complement(1612..3798)
                     /locus_tag="IW748_002040"
     CDS             complement(1612..3798)
                     /locus_tag="IW748_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1104947.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4016..4699
                     /locus_tag="IW748_002041"
     CDS             4016..4699
                     /locus_tag="IW748_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1104948.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4780..5499)
                     /locus_tag="IW748_002042"
     CDS             complement(4780..5499)
                     /locus_tag="IW748_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1104949.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5811..6017)
                     /locus_tag="IW748_002043"
     CDS             complement(5811..6017)
                     /locus_tag="IW748_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104950.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
BASE COUNT         1672 a         1180 c         1061 g         2201 t
ORIGIN      
        1 gttttaacct tccggttttg attggataac cctatgaacc atcgttattt taatttggta
       61 ttaactcatt tcttttgaga taatatattt attggattga gaggctaagc cgttcccccc
      121 ttgggccatt gaacccgcac tcgagagact ggctcctctc tctgataagt ggattgcgta
      181 tgagttagat gttgaaagtc cttactttac tccgtatctc gtacaaggta gtgacgcttt
      241 tcagttgagt ggatttcaat ccaaaattct ttttttgaaa ggtcagatat acatgtactt
      301 tttaggcatt gatatcggta aacgcactca cgttgcttct atcatgaacg aggaaggaaa
      361 ggtattacta aaaggttttt catttcctaa caccactgaa ggagcagagt cattaattga
      421 gcgaatggtc gactattccg gagcacccag tgactttgtt atagggatgg aagccacggg
      481 gcactattgg ctgtccatct tttcatacct tcacgaatca gattatttga ttcacgttgt
      541 gaatcccctt caaacagacg gatggcgtaa aggtacagaa atacggaaac gaaaaaacga
      601 tattattgat tctgttttga ttgctgactt aatgcgctat ggctcatttg tcgaaacggt
      661 cttatccgat gaaaatgttt tttctttgaa acaactttct cgttaccgaa cttatttagt
      721 aggtacagcg agcgacttca aaagaaaaat tatagccgta ttggatcaag tctttccaga
      781 atacgcgacg atttttacca agcagggcgt tttcggaaaa gcttctaaag agttattgct
      841 ggcgtttagt gccccgatcg acttagaaaa catttcctca gaaactttgg cccaatactt
      901 agctgagtta agcaggaacc gaatcaaggc agaaaaagcg gaccaattga agcaagcagc
      961 ttctaactcc tttggagtaa aatttgctca agaagcgttc accttccaac tacgttcaat
     1021 gattgaacaa ttaaaattca ttgaaagcca aataaaggaa acggaaagag aaatcaagca
     1081 cataatggac actttaaatt ctgttattct cacaattcct ggaattggtc ctataaacgg
     1141 tgcgactatt cttggtgaga tcggagatat tcaaaaattt tctaacccga aaaaactcgt
     1201 tgcttacgct gggatagacg cttccgtgac acaatcaggg caatatgagg caacgcataa
     1261 tgttatgagt aaacgagggt ctccatattt gcgaaaagcc ttgttttcgg cggctttagt
     1321 agcttctcaa tgtgatcctg tttttagtgc gttttaccag aaaaaagttt ccgaaggaaa
     1381 acatcatcta actgccttag gtgcggtttc tcgtaagctt tgttacgtga ttcatgctat
     1441 tttgactaaa aacgagcctt atataattgt ttctgattag ctattgaaca ttcaaaaaaa
     1501 ctaatggtaa gaagcttatt tgttgtaccc ttttttcgga attatctgtt ataaaatatt
     1561 ttttgatttg gctaattttt acttgactct atatagttag tctctcttct atcactttcc
     1621 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1681 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1741 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1801 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1861 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     1921 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     1981 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2041 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2101 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2161 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggatat
     2221 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2281 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2341 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2401 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2461 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2521 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2581 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2641 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2701 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2761 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2821 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     2881 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     2941 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3001 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3061 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3121 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3181 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3241 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3301 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3361 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3421 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3481 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3541 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3601 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3661 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3721 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3781 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3841 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     3901 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     3961 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4021 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4081 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4141 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4201 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4261 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4321 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4381 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4441 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4501 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4561 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4621 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4681 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4741 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4801 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4861 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     4921 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     4981 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5041 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5101 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5161 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5221 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5281 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5341 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5401 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5461 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5521 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5581 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5641 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5701 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5761 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5821 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     5881 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     5941 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6001 aattgttcat tttccataag aatccctcca tcacctttta caaggtcagt aattttttaa
     6061 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaa
//
LOCUS       DACENJ010000147         6096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.151, whole genome shotgun sequence.
ACCESSION   DACENJ010000147 DACENJ010000000
VERSION     DACENJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.151"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            193..442
                     /locus_tag="IW748_002044"
                     /pseudo
     CDS             193..442
                     /locus_tag="IW748_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(686..1516)
                     /locus_tag="IW748_002045"
     CDS             complement(686..1516)
                     /locus_tag="IW748_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1104951.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            1724..3058
                     /locus_tag="IW748_002046"
     CDS             1724..3058
                     /locus_tag="IW748_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1104952.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3099..4010
                     /locus_tag="IW748_002047"
     CDS             3099..4010
                     /locus_tag="IW748_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1104953.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4007..4861
                     /locus_tag="IW748_002048"
     CDS             4007..4861
                     /locus_tag="IW748_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1104954.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            4858..5475
                     /locus_tag="IW748_002049"
     CDS             4858..5475
                     /locus_tag="IW748_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1104955.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSFYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            5477..6019
                     /locus_tag="IW748_002050"
     CDS             5477..6019
                     /locus_tag="IW748_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1104956.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVICINCTNKA"
BASE COUNT         1809 a         1092 c         1228 g         1967 t
ORIGIN      
        1 aaaatagatt gccgtaagag atctttttca tttcattttt ttctgaaaac ccgttgtttc
       61 tgtcagtgaa gcagcgggtt tttagattta ttgtctttag acctaattga gcgcgccgag
      121 cgattgaaat acaaaaccac ttgctatgcg ggtggagcaa taaagggaaa tagtttagcc
      181 cgtacaaagt agatgtgtaa ataccacatt gtattcacac caaagtatag aagaaaaata
      241 atttataatc aatatcgagc caatattcag gagatcatca agagattgtg caaatctaaa
      301 atataaattt gggtatcgac acttttgggt ggaaggatac tatgtgacaa aagtaggaca
      361 aaatgaagcg accatcaaaa agtacattca agaacaagaa aaacatgata gtgcgttaga
      421 taagctgagt gtacgagagt aagacgatcc ttttaagggc taaggagtag tacataaacc
      481 ttttaagagg tgggcaaaac tggcaaaggc aatatggctt gaacataaaa tatgaaagcc
      541 agcgtcttta ggcgctggcc ggtgatttga gcttatagct ctagagaaaa ccaccctttt
      601 gaagggtggt tatgattaat gactttagtc agttttgctc acaaagcgtg tggaacaaaa
      661 aacgggtgtt tctacgggta gatatttatt tcttgaatgg aggtgtccca tacatttttg
      721 gtgttactgg ttctaatttt ggtgcagcgc cttcgatctt aggttctttc aaccattgat
      781 attcgccttc gccatccagt gctttgccac tagcccaaga accttcacta ctttggtctc
      841 cctctgagaa gttatagaag tcataagcaa tgtcttttcg ttctttgtca cgcgggaaag
      901 tacttgggac taagattgaa ccttcttttt cttctaattc tttcactgca cattcccata
      961 gattttgatg atagctatct cttgcaagga gaacagacag catatctttt acgccacgat
     1021 catcgatcat ttcgtaaata cgtgctactt gaagtcttcc ttgtgattcg gcatttaaat
     1081 tagctctaaa gtctgctaac aagttgccac tcgcaatgat ataggcaccg ctccatggat
     1141 tgcccacgct gtcagcagga cgaggtccta agccagctac gatcgcgtgc tgtggattca
     1201 ttccagagat aatggcaccg attgttggat ctttttgcat cgctgcttct tgatcttcaa
     1261 caggcgcacc ctctaataaa cgagcaacca ttgtcgccaa catttccacg tggccgattt
     1321 cttctgttgc aatgtccatc agcatatctt tatatttttc ttctcctcgg cagctccagc
     1381 cttggaacag gtactgcata gctacactca tctcgccata ttggccgccg atcagctcct
     1441 gcagatattt cgcgattaat ggattcggac gatctggttt tgcttcaaac tgcaattctt
     1501 tctggtgtct aaacatcaaa attcctccta atctattttt aatacattct cattctgcta
     1561 cgaaagatta aaaaattcaa agaaagcgct cgcgaattat ataaagtatt ggtatcatta
     1621 ataatttttt tagaatcata ccttataaat tgttattttc agaacaaaat gatatcgtta
     1681 tattaaaacc ggcatcggtt acattaaaat ttggaggtaa aatatgaaaa aattattgtt
     1741 tacagggttg ctggcaagtt cagcattgtt tttattagga ggatgcggat caagtaatgc
     1801 aagcgatgcc agttcaagtg cagcaaattc ttctgaaaaa acaagtgatg gaagaaaaaa
     1861 tattgatttc tggtcctttt ggggatccgg tgcacgaaaa gaagctatcg aagaaatcat
     1921 tgaggatttc aatcaatcac aaaacgagat ccatgtaaat tataagtatc agccatgggg
     1981 tgacatctgg actaaatctt tatctgcaat aacagctgga aatgcacccg atgttattgt
     2041 acaggacata aattccgttg cccaaagagc agaagcacag caagcaacta atctctccaa
     2101 atatgtagaa aaagacactt ctaaggattt ttatccgcag ctttgggata cagtagaata
     2161 tgaaggtgat ccttatgcga tcccattcaa tacagataca caagtcattt tttataataa
     2221 aaagttattt aaagaagctg gaatcagtga acaagatcta ccgacaactt gggatgaatt
     2281 agaagaaaca gcacgtaaac tagataaaaa agaaggaaaa gaatttacac gcatcggttt
     2341 ttatccatta tggaatttag gggctgatgt atgggcgcta aacgcagata acgggactag
     2401 ttggtttgac aaagatgaaa aagtcaagat tgatactaaa aataaagtgg aagccttgga
     2461 gtggatccaa gactggcaag attattatgg gaaaaatacg atcaataaat tagaagccga
     2521 atttgggtct ggtgtatctg atccatttat ttccggatta gtagccatgc gtgcccaaaa
     2581 tatcaattat tactctagtt tgatggaaaa tgcaccggat gattttgatt ttggcgtgat
     2641 gcagctccca gaaaaagaag aaggttcagg ccattggtcg tggggtggcg gattcgtctt
     2701 ggaagtacca tacggagcaa aagatcctga ggcctcttat gaattcatca aatatctttc
     2761 ttctccagaa gtacaagaaa aattcggcga aaaaagtttt gatatcatgg cgagcaagac
     2821 agccaatgag aatttagtga ataatgacaa cctaacggat aaaggaaaaa tgatctatca
     2881 aatggcggac gaaaacttta aaaatacggt gatcacacca gttcctttag atgctccgga
     2941 ttacacgaca ttagtgaatg aacagattga ccagattctt ttaggaacaa aatcaccaaa
     3001 agaaggattg gcagatgcac aaaaagcagt agaaaactta gtcaagcaaa atcaataatc
     3061 catttttgac tgtctaggta cagaggaggc aagaaaattt gaagcgttta tctatccgaa
     3121 agaaaaaaga agcaagatgg gcctatttat ttatctcgcc ttttattatc gggttcttgg
     3181 tatttatgct agggcctatg cttttttcag ttattggtag tttcaccgat tataatttga
     3241 catccaagat gaatttcatt ggtctagcaa atttcaaaag aatgttcgtt cacgatgatt
     3301 tattttggaa atcgttatac aacaccgttt atttcgtcat cttcaatgta ccgctgacga
     3361 cgattttcag tgtgttttta gccacagtac tcaatcaaaa aatcaaagga atcagtgtat
     3421 tccgtacagc tttctattta ccggcagtct tatcaggagt agcagtctac atcttatgga
     3481 tgcagctgct ttctccatct gcaggattga tcaacacatt tctgggctgg ttccatatcc
     3541 ctggaccagc ttggctgttt gatccacaat ggacgaaacc tgcgttgatt ttgatgaaaa
     3601 tatggagttc tggaggagca atgctacttt acttagctac attacaaaat gttcctagat
     3661 ctttatatga atcagcagaa atcgatggag caggcgtttg gggaaagttc aaaagtatta
     3721 ctttgccatt aatcactcct attatttttt acgatgtgat cacctctacg atcggttctt
     3781 tccaaatttt tcaagaagct tatgtcatga cgaaaaatgg aacaggcgga cctgcaaatt
     3841 ctttgctttt ttataatctg catatgtgga acaaggcgtt cgtagctttt gatatgggtt
     3901 atgcgatggc tatgtcaatg atcctgtttc tgatcgtgtt ggttctgacg tttgtcaatt
     3961 tgaagcttgc acctaagtgg gtctattatt ctggaggtga cggcaaatga tggattattt
     4021 caaaaagacc agtgtgaatc ggcaaacaaa aaaaatacta agttatgtcc tcatgacagt
     4081 aataggaatc attttgatca ttccattgct ttggatggtg tttacttcat tgaaaccaat
     4141 ggaagaaatc gttcgttatc ctccgacctt tttcccagaa aaaatcgtat gggaaaacta
     4201 tctggatacg attgccgcct ttcctttttg gcgctatgca agaaatacat tattcatcac
     4261 tgtgctagta gtgatcggta atgtcctttc aaattctttt attgcttatg gatttgcgaa
     4321 gcttgatttt cctgggaaaa agctgatgtt tgctttggtt ctttctacga tgatgatccc
     4381 ggggtttgta acgatgatcc ctcaatatgt attattctcc aagatcggct gggtaggtac
     4441 ttatttgccc ctgatcgttc catcgttctt cggcaatgcg ttcaatatat ttctgatgcg
     4501 tcaattttac ttatcgatca ataatgaatt gatcgaagct gctgaaatcg acggagcgaa
     4561 tcatttgtat atttggagtc atttgatgct tccattgacg aaacctgcct tgattaccat
     4621 tgcgatcaat tccttcaacg cagcttggaa tgatttctta ggtcctctgt tatatatcca
     4681 agatcaagaa aaatacacgt tacagattgg cttgcaagtt tttcagaacc aagcaacgac
     4741 acaatggaat tatctgatgg caggagcaac acttgtcttg atcccgacga ttttgctgtt
     4801 cttttttgca caacgttatt tcattgaagg gatggatttg acaggaggtt caaaaggatg
     4861 atgaatcgat cgattggtaa aaaagccaat gattatttct tagaaatcat tgaatggatt
     4921 ccacgtctta tccatttaca gctactatgg ttcctgtgcg tcttgcctgt tttcacatta
     4981 ggaactgcaa gtcgaacagt tctgtatatg atttatcatt acaaaaaaaa tgaagagaaa
     5041 aagctcggtt cactattctg gaaaaagttt cgtgagaact tctcttttta tggaaagcaa
     5101 gatagtttag caagtgttta ttttcttctt ctattgatcg attatcagat tttccgctat
     5161 tggggtgggg gaattggcta tacattgatg tatactacgt tgtttttgct tctattaagc
     5221 gcgcttctct ttagttacaa aatcttgctt caactggaac aaaaagaagc ttcttggatt
     5281 actgcatttt tcttgttttt taatgatttc aaaagcgcgc ttttctattt ggcaggcacg
     5341 ttgatccttc ttgtcgcatt ctggtttgca ggaccaatct attttgcttt attaggtttc
     5401 agtttcttgt tttattttca agtcctgctt tttatccatc gtacgcaaga aaaggttctt
     5461 aaaaaggagg aataacatgt tgcgtttgat tgctaaaatc gatgatagta ttatcaacaa
     5521 gttcgccaag caacggaaaa cggatagtcg aaaaatcaat tttttgaaat ttatcagtaa
     5581 tatctctaat ggtggtttta tttggttgct ccttgctgct gttttagtct tgatacctga
     5641 tactagagaa tatggtctag ccattcttat cggcagtttg atctattttt ttgttggttt
     5701 gctttttatc aaaaaaacga caagacggaa aagaccttat gaaaaaaatc gtcggcttgt
     5761 cctttacaca agtaaaccga agacttcttc ttttccttca ggacatacag cgctagcctt
     5821 tttatcagta gctgcgttgt tcgaattaag tccagttgca ggaatattag cctttttatt
     5881 tgcttgtttg atcgcttatt cgaggatttt tttactttta cattatccta ctgacatttt
     5941 aggaggagct attttaggaa gtgttattgg catcctatgc gctgccgtta tctgtattaa
     6001 ttgtacgaat aaagcatgag tgcgtaaatg cgcatttgtg ctttattttt ttgtttcgag
     6061 gaaagcgtag ttgtttattc atttgtctta ataact
//
LOCUS       DACENJ010000148         6065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.152, whole genome shotgun sequence.
ACCESSION   DACENJ010000148 DACENJ010000000
VERSION     DACENJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.152"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            53..430
                     /locus_tag="IW748_002051"
     CDS             53..430
                     /locus_tag="IW748_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1104957.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            418..756
                     /locus_tag="IW748_002052"
     CDS             418..756
                     /locus_tag="IW748_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1104958.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            890..1333
                     /locus_tag="IW748_002053"
     CDS             890..1333
                     /locus_tag="IW748_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1104959.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1595..2410
                     /locus_tag="IW748_002054"
     CDS             1595..2410
                     /locus_tag="IW748_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1104960.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2420..3082
                     /locus_tag="IW748_002055"
     CDS             2420..3082
                     /locus_tag="IW748_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1104961.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3287..4951
                     /gene="alsS"
                     /locus_tag="IW748_002056"
     CDS             3287..4951
                     /gene="alsS"
                     /locus_tag="IW748_002056"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1104962.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            4964..5674
                     /gene="budA"
                     /locus_tag="IW748_002057"
     CDS             4964..5674
                     /gene="budA"
                     /locus_tag="IW748_002057"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1104963.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            5804..>6065
                     /locus_tag="IW748_002058"
     CDS             5804..>6065
                     /locus_tag="IW748_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1104964.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQ"
BASE COUNT         1971 a          960 c         1302 g         1832 t
ORIGIN      
        1 taaataaaat acataagtaa agtggcaaga aattaagaga aagagtgatc agatgaatat
       61 caaacaagtc agtgaagaaa agggcatttc ggccgataca ttgcgctatt atgaacgaat
      121 cgggttgatt ccaccagtta atcggacaaa aggcggaatc cgtgattata cagaagaaga
      181 cctaaaatgg gtcgacttta cgatctgtat gcgaaatgcc ggattatcga tcgagtcttt
      241 gattgaatat attcgcctat ctagcgaagg agaagcaact gtatttgagc ggcgtaagct
      301 tttgattgaa gaaagtgaac ttttgacgaa aaaaattgct gaaatgcaag aatgtttgga
      361 acgtttacaa ggaaaaatca agaaatatga tcgtgtgtta tctagaaata aaatggaatg
      421 tatcaagtaa aaaaatcaag gagcatcgct atggagttac gcttactaaa atatttttgg
      481 acaattgcag aagaaggaac aatctctcaa gccgcagaag ttctacatct tacacaacca
      541 accttaagcc gacaactgaa ggagttagaa gaggaactag gaacagaact gtttgtacgc
      601 gaaaaccgca aaatgatctt gacagaagca ggttattttc taaaaaagcc gagcagaaga
      661 aattttagat ttaacgaatc aaaccactca agaattcgag aacagaaaaa aacaattatt
      721 cagcggccga tttacgatcg gctgtgtgga agctgataat tcgaatacat tggcactatt
      781 actagaaaag ttgatccgtg attatcccag cgttcaattt catatccaca gcggaacaag
      841 tgatgatatc acggagtgac tagaaaaaag cctgttagat ttagcgattt tgctagatcc
      901 agtagataca gagaaatacg aaacgatttt gttaccaaga aaggaaaaat ggggattgct
      961 cattttagaa aattctgttt tagcaaaaaa agacgtgata acaaaagaag actttgcgaa
     1021 tgtacctctt ttgatctcaa agcgtccaga aatacagcga atgtttgctg actggttagg
     1081 cataaaaaag gagcagttgc tgatcacagg aacgtacaat ttgattttca atgtatttgc
     1141 gctaattgag aatcaggtag gtgctgcttt agtcatagaa ggtgctgtaa gtcatctttc
     1201 aaagcgagtg acattcgttc cgcttgaccc aatgattgaa acgaactgca tgctagtctg
     1261 gaaaaagaat cggattttat cacctgtagt caatgaattg attcaccgtt tcaaagaagc
     1321 ttttgaacag taataagtat ctctaatcga agaaatttca atacaaaagc gagacgtatg
     1381 acagtcttgc ttttgtattt tttcattttt aaatcaaaaa aaataaagag atttacataa
     1441 cttgttaaat aagcgcttac aaacataaga agaacggaaa tgaatcagct ataataaccg
     1501 ttgttctgga ttgtaatcat tacaaagaaa tgaagattat aactttggag gtgttcgaaa
     1561 tgaaagaaaa aaagcaaaaa ttcgttcaaa aaacatgccc tttattcaat ggttgggtat
     1621 gattctgctg attgtattag ctttattgac gatcattcct tttttatgga tggtttctgc
     1681 ttcattcaaa gcaaataatg aagtgttcac gattccgatt caatggatcc ctaaaacttg
     1741 gcatttagag aattatctgg ttatttggtc tcgtattcct ttagtgacat tttttaagaa
     1801 tacagctttt ttaagcgttg tgatcacgtt gattcagttg tttacttcta gttttgctgc
     1861 ttacgggttt tctaaactaa attttaaagg gcgcgatatt ttatttttag catatatcgg
     1921 cacgattgct gtcccttggc agtcttatat gatcccgcag ttcattatga tgagaaaagt
     1981 gggtttaaca tatacgttgt ggtcgttggt gctattacaa gcgttcaatg cttttggtgt
     2041 gttcttgttg aagcaatact acagtagtat cccagattct ttatgtgaga gtgcgcggat
     2101 cgatggctta tcagagtggg gaatctatcg caagatcatt ttacctctta caaaaccggc
     2161 gattgccagt ttgacgatca tcacattcgt taatacatgg aacgattata tggggccatt
     2221 catttatttg tcttctacgg aaaataaaac aatccagtta gggttgaaaa tgtttattgg
     2281 gctatttgat gcagagtacg cattaatcat ggctgcctca gttgtttctg ttcttcctgt
     2341 tgcttttata tttttagcga tgcaaaaata ttttgtagaa gggatcgctt catcaggaat
     2401 gaaggaatag gaggtgatta tgaaagagca gcgttttgac aaaaatgttt atatggaatt
     2461 cttttattgg ttgtatcaat cattggcaag tagcctttgt ttttggtgtg tcaatatctt
     2521 gtatatagca gctgcgttcc ttttgtcgat agatggaagg aatgtctttt cctttatttt
     2581 ttctttactt tttattggtc caggttgcat cgtgatgata ggtatgacag aagaattagc
     2641 aaaagaaaaa agtttttcac cagtacgttt gttcataaag acgtttcgcc aattttggct
     2701 aaagggattt ttttattggc tatttgcttg gatagttagt gtgataatga tttttgattg
     2761 tttctttttt atacgttttt cttatggaaa atggttgatt ccgttgtttg ttttacttgc
     2821 ttgtttatca gtttccttta gcatcaacta ctggtatttt caggtgagaa atccagcgtc
     2881 gaaaccaaat caagttctac ggatagcgtt ctactatacg ttgaaaaaat ggtatgtgag
     2941 tttactagat tttttactat tgacaagttt gtttctcttc tttttcgtaa agccccaatg
     3001 gtgtattcta ctaggtcctt ctatcgtatt tggtttgatt tacttcaaca atcgtaaatt
     3061 gatgagaact atggatctgt agactttata aattgaaact ttcaattaag aatgacagtt
     3121 tttatgtaaa aactgtcatt ctttttcatt ttttgaaaca tatggctaaa caaatgaaat
     3181 aaaacgtgct atacttttga atgaaacagg tttagaaact gtatcaatta tttgtgatta
     3241 tttctaacta ataattaaaa ataaagcata aaaaggacga tgttcaatgg acgaaaaaat
     3301 gaaacaaggt tcgaaatctg tagtagaaag tttgattaat catcatgttg attatgtatt
     3361 cggtatacca ggtgcaaaaa tcgatggtgt atttaatgaa ttagaggatc aaggtcctga
     3421 attaatcgtg actcgtcacg aacaaaatgc tgcatttatg gcacaagcaa tcggacgtat
     3481 tacaggagag ccaggagtag ttatcgcaac aagtggtcct ggtgctagta acttagctac
     3541 aggtttggta acagcgacag cagaaggtga cccagtttta gcaattgccg gacaagtaaa
     3601 acgaagcgat ctattgaagt tgacccatca aagcatggat aatgcagcac tgttccagcc
     3661 aatcacaaaa tacagtgcag aaatccaaga tccagaaaca atttcagaag tgattgccaa
     3721 tgcttatcgt atggctaaga gttcaaaaaa aggcgcgagc tttatcagta tcccgcaaga
     3781 tgtagtggat gcacctgtcc aagggaatgt catcaagcca ttgagcgatc ctaaattagg
     3841 ttctgcttct gcagatgata tccgttacct agctgaacgc attagagaag caaaactacc
     3901 ggtactatta gtaggtatgc gaggatcaag tgaaaaagaa acgttagcca tcaggcaatt
     3961 agttggaaag acagcattgc ctgttgtaga gacattccaa gcagctggag tgatctcacg
     4021 tgaattagaa gcgcatttct ttggtcgtgt gggtttattt agaaaccaac caggagatat
     4081 gcttttgaaa cgaagtgatc tagttattgc tattggctat gatccaatcg aatatgaagc
     4141 aagaaactgg aatgcggaaa aagatgcgcg gatcattgtg atcgatgaag cgccagctga
     4201 aatcgatcct tttatgcagc ctgaaagaga attgatcggt gatatctcag ccactcttga
     4261 tttattgaca ggttcattag agcctcaaca agtgtcagaa gatgcaaaag aatatttagc
     4321 atctcttcaa gcagaattaa cggagcggga cattgttcaa tcaaaaggag aagcaggtat
     4381 cttgcatcct ttagaagtta ttaacacgct tcagtcaaaa gtaacagatg atatgactgt
     4441 taccgtagat gtaggtagtc attatatttg gatggcacgc cactttagaa gttatgaacc
     4501 tcgtcatttg ttattcagta atggtatgca aacgcttggt gttgcattgc catgggcgat
     4561 ttctgctgca ttggtgcgtc caaatacaca aatcgtatca gtttccggcg atgggggatt
     4621 tttattctct gcccaggatt tagaaacggc ggtacgtaaa aaattgaata tcgttcattt
     4681 gatttggaat gatggacact acaatatggt agagttccaa gaaaaaatga aatatcagcg
     4741 ggcgtcaggt gtagactttg gtccagttga ttttgtgaaa tatgcagaag cttttggcgc
     4801 taaaggaatc cgagctacaa gtgtagagga attagaaaaa gctttagaag aaggatttgc
     4861 gacagaagga cccgtaatta ttgatattcc gattgattac cgtgataatg agaaattggg
     4921 agaaacaatc ttaccagatc aattctatta aggaagtgtc gacatgactg aaaaaatctt
     4981 ataccagcac gggacattag gagctttgat ggctggcttg atggatggaa cagaaacgat
     5041 tgctcatata ttagaaaaag gaactctggg attgggaaca ttgcatgggc ttgatggtga
     5101 agtcatcttt ttagatggcg tagcttatca aggaagatca gatggctcag ttgcccaatt
     5161 agatggaagt gaacttaccc cctatgcagc aattacagat tttacgccag atactagttt
     5221 ttctgtctca aaaactgcag atggagaaca attgaaaaaa gacatattag taagagaagc
     5281 cggggaaaac ctatttttgg cagtgaagat aactggatta ttcaaaaata tgcacatccg
     5341 tatcatgccg aagcaagaaa aaccttacag aagactagca aaaatttcag agtctcaacc
     5401 agaattccaa caaagcaatg tccaaggaac attagtcggg ttctttacgc ctgaattatt
     5461 ccaaggagtt gctgcagcgg gatttcactt gcattttatc gatgatacac atacatttgg
     5521 cggacatgta atggattttg aagtggctga aggaaaagtt gagattagcc gcatctcgtc
     5581 ccttgtacag cattttccag ttgatgatcc gacttttgtt cacgaaacga ttgattatgc
     5641 tgatttagca gcagaaatcc aacaagcaga atagaaaaag acagcgagat gcgttcaagg
     5701 tgattaacct gacgtatctc gtttttttga ggaggaaacc tagcgtttac aaaaaaactg
     5761 agctataatc ttctccatag atcaaaaagg agcggttttt atcatgtgga cagaaaaaca
     5821 gaaaaaagat gcatacgaaa ttttattatt acagcaacaa ggattattag aagcagaaga
     5881 taactggtta gcaaatttag caaattcatc tgcattgttg aatgagactt tgcctgagac
     5941 tgtctttgca ggatattatt tattcgatgg taatgaattg attttaggac cgttccaagg
     6001 gcgagtttct tgcacacgta tcaagatggg aaaaggagtt tgtggagaat cagctgaaaa
     6061 acaag
//
LOCUS       DACENJ010000149         5978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.153, whole genome shotgun sequence.
ACCESSION   DACENJ010000149 DACENJ010000000
VERSION     DACENJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.153"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            190..546
                     /locus_tag="IW748_002059"
     CDS             190..546
                     /locus_tag="IW748_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1104965.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            556..1455
                     /locus_tag="IW748_002060"
     CDS             556..1455
                     /locus_tag="IW748_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ1104966.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            1972..2703
                     /locus_tag="IW748_002061"
     CDS             1972..2703
                     /locus_tag="IW748_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1104967.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2848..4875
                     /locus_tag="IW748_002062"
     CDS             2848..4875
                     /locus_tag="IW748_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ1104968.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            4983..>5978
                     /locus_tag="IW748_002063"
     CDS             4983..>5978
                     /locus_tag="IW748_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1104969.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKD"
BASE COUNT         2068 a          999 c         1243 g         1668 t
ORIGIN      
        1 aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc
       61 tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata aattgagaac
      121 agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct atgacatgag
      181 gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa gaataaatta
      241 gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt tgcagaaagc
      301 caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa agatacacaa
      361 acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat caaaatcaaa
      421 gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc aacttataat
      481 agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa
      541 gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt ggacatcaat
      601 aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca caagatcact
      661 cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc tgttgtcgaa
      721 tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga gttggcagat
      781 gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa tgaacggtat
      841 attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga ctatcagttt
      901 tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg
      961 ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa
     1021 aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt
     1081 cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa caaattaaat
     1141 gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa tatcaaacaa
     1201 gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca cacgatttac
     1261 agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt tgtcttgaaa
     1321 gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg tgcccatcac
     1381 ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca agcgatgaca
     1441 atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg taaaacataa
     1501 ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt gcggaaaata
     1561 atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag atgcaagcgt
     1621 gtattctttt tttgaacgat aatcacaaaa aaaaatataa tatacatttt ttatttaatt
     1681 ataattaata ttgattgaag tctaggcagt caagtcacat tttgatagat aggaggggga
     1741 cttacgaatt attttaaagg ctatgacagc tgtatttcta gattgtgttt gcatcaagtt
     1801 gcaataaaaa ctagaaatca aatcgggctg ataatcattt ataactaatc gatacatagg
     1861 accaaatata ggtctatgtt cttttatttc ataagtatcg taaaacagaa ttttgctatg
     1921 gattaatcca atcgactgat atgtttagta aataaagaaa gaggaatgtc aatgatgaaa
     1981 aaagaaatcg tattaacagc agcaatgatg ttattcagca tggtagcaag tacgactttt
     2041 gtttctgcaa ctgaagtata cccaaaggaa tataacacag aaggaacgat tacatttgaa
     2101 gcaggagatg agggtgtaac tcctcctgtt gatccagaga atcctgatcc caataaacct
     2161 gtcgatccat cagatcctcc aagccctgga acaggagggg cattatcgat tgattatgga
     2221 tcaaaattca aatttgggac tcaaaaaatt tctactgcgg ataaaacgta ttatgcagct
     2281 gctgatgtga tgaacgatgg gtctagaaaa ccgacatatg tccaagtgac agataggaga
     2341 agtaccttat ctgggtggaa attaagtgtc tctcaaccag aacagtttaa aactgccagt
     2401 ggggatgaac tagttggagc acagctgaaa tttacgaaag ggcaggctgt ttctttggtt
     2461 gatccaacat atacgccgca aacggtgaat tctgaattaa cgttgacacc gggaggaaac
     2521 aatacgttag caattaatgc taaatcagga acaggtgtgg ggacttgggt ctatcgattt
     2581 ggtgcaaatg aaaatgaaaa tcaagatgcg gtacagcttt cggtacccgg taaatctgtc
     2641 aagttagcgc aacaatattc cactaaatta gtttggacat tagaagatac acctaacaac
     2701 tagtttactg aaaagaactg ttaaaaataa atacgtagtc atattgtctt gcgaggtaat
     2761 cgagcaaaaa ttgtcaaagg ctaggctaaa tttgtttgga tacgaatagt tcatctagga
     2821 atttaacgat caggaggaaa agatatattg aaaaagagga ggatcgtgct cattttaagc
     2881 agtctcttag tgactaatat ctttccgatc atagggtact ctttggaatt gaatcagttg
     2941 gagcatggaa atagaagaga cgaagaaagc aaactcactt cgacattcga cagtttaata
     3001 gaatccaacc agacacaaga aagcagtgag gatttaagta tttcgagtag tggagagacc
     3061 actgacaacg tagaagctga gggttcggac aaacaaggac tagaagatgc accaatttct
     3121 gcggttgagc tgcctgacga tcccaatatt atttctatcg ataaaatttt tcaaacacct
     3181 atcggtacta gcacgtccat attggaaaat gggaagttac tgcagttgaa cccagcagaa
     3241 aggtctcaaa gaggagctat ttggtcaaat aagaagatta gtttactctc ggattttaca
     3301 tttaaatctt atttatactt aggaaatcaa cgagatagag cgggagatgg catgaccttt
     3361 acattgactg gcgatcctag aatggctagt aatcccagtc aagttattgg atcgccaggt
     3421 atgggaattg gctcatattc tactcgttct ggccaacctt acattgcgaa tgcgctctcg
     3481 attgagtttg atacgtataa aaatagtggt tcttctgatc gaatggatcg agagataact
     3541 gctgataata gagggcatgg acatgtcgct tttgtcacgc ctaaaccaaa taataataat
     3601 tattctggag agcatactgg cgtgacggta gctcctacat ttctttctaa tggcacatgg
     3661 cgcttgttaa taattaaatg ggacgcggag caaagaagtc ttacttatga tttggaaggt
     3721 gtcggtcgct cttctcggat tatacctgat ctcattaggc agtttggttc tacagaagta
     3781 aattgggggt ttacttcttc aacaggtact ttttttcaag aaaatgctgt cgctgtcacg
     3841 cgacttccat cgaatgtcac atcaacagct gatgtaaaag tcaacgatag tgattatggg
     3901 gccatcgctg aagctggagg aaacgataag ataactatca gaaatacatt gaaggtcgag
     3961 ggggattttc ttgaatataa gaaaatgcag actagtatcg acctcccaca agaattaact
     4021 attcccgagc atatcttgat caatggaaat caaattgatg ccaaagatat cttgataacc
     4081 ggaaatcgag tcacgctaaa tctgacagat tattttattg ctggagatac gttaataatc
     4141 gaattggaga caactttgca ggaagtaatt cctgaaaaag tactcactat gcattttgaa
     4201 taccttgaaa acgggaattt gattgagcaa tcgaatgaaa taaacctaaa gatcgcaaag
     4261 ctgaaggaga tagatatcca tgtttattat aaagataaag acagtcagat tgatattgct
     4321 gaaatgaaaa caatcactgg gaaaatagga gagaagtata aagaaactcc tattgatatt
     4381 gaagggtatg tttttgactc ggattcagcg aatacagaag gcatcttctc cgaaaataca
     4441 gaggatatct atttttatta cagaaaaggc aaactttatc tctcaactgc tcctcaatat
     4501 gtcaattttg gaaaacataa aatatccagt agaccattaa gtgaattcgg acagttttca
     4561 ggtgggttga aaattataga tgaacgggct tttggaagtt ggaaattaca attgaaacaa
     4621 gagaaagttc ttacaaatgg agattttgag cttccagaag ctctttcctt tgttacctct
     4681 gaaaatacaa cggttatagg gacatccgct gttacgattt tcgaaagtga tcaaaagggt
     4741 gaaagcgatt tgtcctctct tttagattct acagctcatc gtggaatcag aatagatatt
     4801 cctgtagaaa accaaagaat cggaaccttt gaggggaaac tatcttggat acttgcagat
     4861 gtcccaggaa attaacagga agagaagaat ataacaaaat tattgagttt caagaagggc
     4921 acaggtacca taggctcttg tgtcccacag agacaagatt ttgttatagt cttctcttac
     4981 atatgattaa aggagcgatt ttcttgagac caaaacaaca attatttata acactcctag
     5041 ttattttagg ggcacttttt aacaatatat cagtgtcagc tagtgcattt aatttttcag
     5101 tgacgccgat aacttcagag aatcaaatcg acaagagaaa aacttacttc gatcttcaac
     5161 ttgatccgga tcaggaagta gaagtaaaag cggaattgag aaatgataca gaaaaagaag
     5221 tgaaaatcga tatttctgtg aatagcgcaa ccaccaattc aaatgtgatg gttgaatacg
     5281 gaaaaaacga gattgaaaaa gacatgagcc tgatctttga tctggtagat tatgtgagct
     5341 atccacagat agtgacgcta aaacctaaaa gtgtccaaac cgttgctttt catgtcagga
     5401 tgccgaatga gcgatttgat ggtgtattgg ctggtgggat cacattccaa gaaatccaga
     5461 cggaaaagga ccaaacagaa actaaggatc aaagcttatc gattgagaat gaatacacct
     5521 atattattgc tttgttgatg caacagacac tgaacgaagt tgctccgaac cttactcttc
     5581 atgaagttaa accagatcaa atcaatgcaa gaaatgtcat cttagcaaac gttcaaaatg
     5641 atcaaaaaac ctatatcaat caagtagtta ttgaaacaaa aattacaaaa aaaggacatt
     5701 ctgaagtttt atatcaagag gaaaaagaag gattgcagat tgcgccgaat acaaattttt
     5761 cttttccaac tgctttaaat ggtcaaccgc taaccccagg tgaatatcac cttacaatga
     5821 ctgttttagg aaatgaaaat gaaaatggaa aattttcgag aaaaaaagga aatactacga
     5881 tcaactatac caatcaatgg gtatttgaaa aagattttac aatagacgga aaagtagcca
     5941 aggaattgaa cacgacggat gttacgatta aaaaagac
//
LOCUS       DACENJ010000150         5958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.154, whole genome shotgun sequence.
ACCESSION   DACENJ010000150 DACENJ010000000
VERSION     DACENJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.154"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            281..805
                     /gene="infC"
                     /locus_tag="IW748_002064"
     CDS             281..805
                     /gene="infC"
                     /locus_tag="IW748_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1104970.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            877..1077
                     /gene="rpmI"
                     /locus_tag="IW748_002065"
     CDS             877..1077
                     /gene="rpmI"
                     /locus_tag="IW748_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1104971.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1130..1489
                     /gene="rplT"
                     /locus_tag="IW748_002066"
     CDS             1130..1489
                     /gene="rplT"
                     /locus_tag="IW748_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1104972.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1741..3048
                     /locus_tag="IW748_002067"
     CDS             1741..3048
                     /locus_tag="IW748_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1104973.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAIFA"
     gene            3125..5254
                     /locus_tag="IW748_002068"
     CDS             3125..5254
                     /locus_tag="IW748_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1104974.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKQQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            5229..5918
                     /locus_tag="IW748_002069"
     CDS             5229..5918
                     /locus_tag="IW748_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104975.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
BASE COUNT         1925 a         1060 c         1323 g         1650 t
ORIGIN      
        1 gggaatcact gctagaaagt ctgctaatct aatcattttc agaaaaggaa gacaaactgt
       61 atgaaaaaca ttgacaaaaa gattagtttt tgctatacta tgatggttga gaaataaaag
      121 cagaagcacc cgcttctcgc cttagttgat aatgtcactg ggctagatag ttttcactgg
      181 ttctattagg tgaagtacgc gcgggttctg taagagaact cgttttttta ttgccgttta
      241 ttcggtaagc ttttctttct ctcataatat ttggaggtga atgaccatag caaaggatat
      301 gatggttaac gacggcattc gtgcacgaga gttacgattg atcggttcag atggtgagca
      361 attaggagtt aagacaaaag cagaagcatt gaatattgca gaacaagcaa acttggatct
      421 tgtgttagtt gcccctggtg cgaaaccacc agttgcgcga atcatggact acggtaaata
      481 ccgtttcgaa caacaaaaga aagaccgcga agcgcgtaaa aaacaaaaag tgatcaatgt
      541 aaaagaagtt cgcttaagtc ctacgatcga cttgaatgac ttcaatacaa aacttcgtaa
      601 tgcacgtaag ttcttggaaa agggcgataa agtgaaagct tctatccgtt tcaaaggccg
      661 tgccattacc cataaagaaa ttggtcaaaa agtcttgaat cgcttagctg aagaaactgc
      721 ggatattgca acagtggaac aaaaagcgaa aatggacgga cgcagcatgt tcttgacgct
      781 ggcaccgaaa aacgacagta agtaatttga atgactaact ggctgttgga cagccgatag
      841 tcggataacg aaagattttg aggaggaaat atagtcatgc caaaacaaaa aacacaccgc
      901 ggatcagcaa aacgtttcaa acgtactggt aaaggcggat tgaaacgctt ccgcgcgttt
      961 acaagtcacc gtttccacgg caaaacaaaa aaacaacgcc gtcaattgcg taaagcaagc
     1021 atggtttcta gtggcgactt caaacgtatt cgccaacaac tagcaaaaat gaaataagaa
     1081 gtacaaataa cagaatctga agatttattt ttattaggag gaattataca tggcacgtgt
     1141 taaaggtgga acagtaactc gtaaacgtcg taaaagagtg ctaaaattag caaaaggtta
     1201 ctacggttca aaacacactt tatttaaaac agcaaaagaa caagtaatga attcatacaa
     1261 ctacgcatat cgcgatcgtc gtcaaaagaa acgcgacttc cgtaaattgt ggattgctcg
     1321 tatcaacgca gcagctcgta tgaatggctt gagctactca aaattaatgc acggtttgaa
     1381 agttgctgaa atcgacatca accgtaaaat gttagctgac ttggctgtta acgatgcagc
     1441 agcatttact gcattggctg accaagcaaa agaagctttg gctaaataag ctaaacaatt
     1501 ataaagagga aagaagataa cgaaaagtta tgttctttcc tcttttttta tttacaatcc
     1561 tccatcaaat gaaacaggac aataacgtat aagtctaaaa tagcataatt aaggtttttt
     1621 aattgaaaac tgaacattta tggtcaccct ttaatgaaag cgctaactac ataagttaga
     1681 atgagtttga ctgatatatt agtttgctta tcagaaaaac aaaatggacg gaggaataac
     1741 atgagtgata ttattattgg tattttgtta gttatctcat tctttttcat ggtctggtac
     1801 tgtgtaaaag gctacaactt gatggtcggc tttgccatca tggctactgt atggatggga
     1861 ttagcgctag tcggaaacac gttttcacca aacccggcga tggaagggca aggagtaatt
     1921 gatgtgttga cgcatatcta tacgacgggg ccagctgagt acgccaaatc gatcttggta
     1981 aatgtcttct ttggtgcctt tttcgggcgt gttctagtag atagcggaat cgcagctacc
     2041 ctgattcgaa aggtagttga acttggaggg gacaagccaa gaatcacgat gagcttacta
     2101 tgtattgtaa ccgctgttat attcatgtcg atgacaggaa tcggaccagt gatttctatt
     2161 gcagttattg tgttgccaat cttgatgtca ttaggtattt cagtaccggt tgcactgttc
     2221 tcatttatgg gctcgattat ggctggaatc tttgctaata tcgtcaattt caaacaatat
     2281 caaacgattt atgctggttt caatccagcg gctgaaagct atacttataa tgactatttc
     2341 caaattggta tgattgggat gattgtaagc ttagttgtgg tactaacggt cgctaatata
     2401 tcaatgaaca aaaagaaacg ctatgcaatg gcagcaaacg taccagctga aggtggcgac
     2461 gcaccaatga tctcatggtt agccgtatta ttaccagttt taggggtcgt tcttttagat
     2521 cttccaatca tcttaggttt tattctagca ggtatctggg cattgctgtt tactggaaaa
     2581 ttgcgcggag gttataaaga aatctgtcgt cagtttgcga aattatttac agatggcgca
     2641 gtagatgttg caccaatggt tggtttcttg atgaccttag cgatgttcaa taattcagct
     2701 gcgtatgctt ctccttattt ttcagctatt tttggagatt ggataccgca atcaccatta
     2761 gtcttggcaa tcgtatttgc tatattgaca ccactaggat ttttccgcgg tccaatgaat
     2821 ctagtcggtt caggctcagc tattttagct gtcgtattag ctgttaatcc aacgatgtcg
     2881 ccagcctttt tgttcccgtt atttgcaatc acaacgattg ctcctcagca tttagatatt
     2941 acacaatctt gggttgcttg gggattaggt tatacaaaag taacttctcg agaatatatg
     3001 aagaaatcca tcccaactgg ttggatcatc ggagcgatct ttgcctgatc acgttcttat
     3061 tgtacgggaa cgcctagtaa acaaaacgtc gataaaaacg attagatgaa tggaggaaaa
     3121 gaaaatgaaa cgttttgtgc gaatgggaat tgatgttggc ggtacacata cgaaagcagt
     3181 cgcaatcgat aatgcgacgc acgaaatcat cggaaaatct tcagtgaaaa caacccacga
     3241 tgatgtccgt ggagtagccg caggtgtcgt tcagtctttt caaaattgtt tgcgggaaaa
     3301 taatatcagt ccagaagatg ttgtgtttgt tgcacattct accacacaag cgactaatgc
     3361 actgattgaa ggagacgtag caaaagtcgg cgtaatcggg atggcaaaag gtggattaga
     3421 aggtttttta gcaaaacaac aaactagatt gaacgatatc gatttaggga ataagaaaaa
     3481 aatagagatt gtcaatgcat tcttacctgt caaacactta aatgtagacc gtgtttcaga
     3541 aacgatttct tctttagaaa gagaaagagc ggaggtcttg gtttcttcta tggcatttgg
     3601 agtagataat ggggaaccag aacgagttgt ttatgaagca gcatcggtaa aatcgatccc
     3661 tacaacgatg gcttctgaca tcacaaaatt atatggattg acaagaagaa ctcggacagc
     3721 agcaatcaat gcatctattt taccaaaaat gctagatacg gcaacctcta cagaagattc
     3781 cgtacgagaa gcaggggtca atgtttcttt gatgattatg cgcggagacg gcggagtaat
     3841 ggaaatcaat gaaatgaaaa aacgacctgt tttaaccatg ctttccggtc cagcagcatc
     3901 agtcatgggc tctttgatgt acttgagagc gtctaatggt gtttattttg aagtaggcgg
     3961 aacaacaacc aatatcgggg tcattaaaaa tggacgtcca gcaatcgatt attcaattgt
     4021 aggtggccac ccaacatata tctcttcatt agatgttcgg gttctaggtg tggcaggcgg
     4081 ttcgatggta cgggctaatc agtcaggaat catcgatgtc ggtccacgtt ctgcacatat
     4141 tgccggtcta gattatgcag tatttacgga gacagaaaaa atcaaaggtc ctaaggttga
     4201 atttttctct cctaaagaag gagaccctgc tgattacgtc aaggtagtga tggaagacgg
     4261 agaagaagtg actattacaa atacatgtgc agccaatgtc cttggattag tgcaagaaga
     4321 acatttttct tatggaaatg tcccatctgc acgaaaagcc atccaagcat tagctgatta
     4381 ttgtcatacg acggttgaag acatcgcaga acagatcatg gaaaaatcct atgctaagat
     4441 tgaaccggtg attttagaat tggcagacaa gtatcacttg gagaaagatc agatttctct
     4501 tgtaggtgta gggggcggcg ctgcctcatt gattacttac tttagtaata aaatgggtgt
     4561 gaaatattcg attcctgaaa acgccgaagt gatttcttct attggtgtag cacttgcaat
     4621 ggtccgagat gttgtagaac gtatcatccc ttcgccaagt aaagaagata tacgatcgtt
     4681 gaaaaatgaa gcgatgaata aagcgattga atcaggcgct acaccagaat cgatcgaagt
     4741 ccacgtagaa atcgatccac agacttcaaa agtaacggct atcgctactg gttctacgga
     4801 agtgaaagca accgatctaa ccaaggagat cacaacagaa gaagcactag agcttgctgc
     4861 agaggatatg cgattaaata aaaatgaagt ttgcttattg gaaaatacac ctttctttta
     4921 tgtatgcggc gaacagaatc gttcaaaaaa tgctggaagt cttcggatca tcgatcaaaa
     4981 aggatttatc aaggtccaac gtggacatgc ttcatgtatg aaaacaactg cagccaatta
     5041 tatgacagct gtcgaacaac tttgggaaga tatggcagtt tatcaaactg aattgattgc
     5101 ccgaccagaa ttttatctct gcttaggagc aagagtctct gattttacag cgacggatct
     5161 ggaacaacta cagcttttga tggatctgga agtatctact atggaaccgg aggaagaagt
     5221 gatcgtcgtt gccggaaata tcaaacaaac ataagttaga agagttacta aacgaattac
     5281 aacagatccc cgaagaaatt tggggattct accagtttcg gcgagattta ttttggaaaa
     5341 agattccttt gtccaagcag aaaattctta tacaacaatc aatcgattgt ggtatcgaaa
     5401 cggcatgtag catcaaaaaa aagtaccctt ttgccgatgt aggagaaatt tgtgaacaaa
     5461 tggctgttcc aatcgtttcc tgtgaatcag agcaaataaa cgaacgaatc acttttgcaa
     5521 cttatgcaga agatgaaggg attcgattga tgacggagcc gttagaaaaa ttaaaatgtt
     5581 caggactagc ttcgatctct aaagaaacag cgcaagcttt gattattgga catgaactat
     5641 ttcaccacat cgaagcttct gttaagggaa tttatacgca aagcgaaaaa attgtgctgt
     5701 ggcggttgcc tttttataca catcaatcaa acattcgtgc attgagcgag atagcagcga
     5761 tgagtttttc aaaagagatg aatcaaagcg atttttcgcc ttatgtatta gaggcagtct
     5821 tactttggcc atataatgaa attcgtagcc aagggatctt agaagaaata aaaaaaatag
     5881 aaaaaagatg tacggagtat gatttagatt acaaataaag agactgtgac aaaagtcgtg
     5941 tcacagtcct ttttttcc
//
LOCUS       DACENJ010000151         5958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.155, whole genome shotgun sequence.
ACCESSION   DACENJ010000151 DACENJ010000000
VERSION     DACENJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.155"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            184..2277
                     /locus_tag="IW748_002070"
     CDS             184..2277
                     /locus_tag="IW748_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1104976.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            2302..2604
                     /locus_tag="IW748_002071"
     CDS             2302..2604
                     /locus_tag="IW748_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1104977.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            2631..3920
                     /locus_tag="IW748_002072"
     CDS             2631..3920
                     /locus_tag="IW748_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1104978.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            3939..4346
                     /locus_tag="IW748_002073"
     CDS             3939..4346
                     /locus_tag="IW748_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1104979.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            4369..5238
                     /locus_tag="IW748_002074"
     CDS             4369..5238
                     /locus_tag="IW748_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1104980.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            5240..5860
                     /locus_tag="IW748_002075"
     CDS             5240..5860
                     /locus_tag="IW748_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1104981.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
BASE COUNT         2009 a         1023 c         1265 g         1661 t
ORIGIN      
        1 tgtatagatg ggcaaaattt ttgtaataat aggcgtatgt ctttttcgtt gaggagatga
       61 aaaagcatat ggcttatttt ttgtttgctt tgcttacttt ctttccactt caagtggcag
      121 aaatatctac tttttattat gtaagcggaa ccatatactt gaaatcagaa ggagatcatt
      181 tctatgaaaa caagaacaat tgaaatactt cacagaatca tgacagccca atatccagta
      241 aaagcaaaac atctagcaag tgagttcaat gtcagtcagc ggacgatccg tcaggaagtt
      301 cttgaagcga atatctggtt gagcagtaaa aagcttccgg agatccgtac aattcgcaac
      361 aaaggttttt tgttgaagct ggaaaaggaa aagcaagctc gattagaaac tgaaataaaa
      421 gatgtgaaag acggcttttt gaatcggcgc gaacgtattt ttgatctagt tttagccgtt
      481 gcttacagcc gaatcccgat ccatctaaac aaaaaagaag aagaatttca aatctctaaa
      541 agtacattag atgaagatat gagacggtta cgagcaacac tggtcagtta tgggatagag
      601 atcgtcagtt atggaaagca aggactggtt tataaaggtt cagaacgatc tatccgtacg
      661 atgatctatg attttatcaa tcagaatctg ggaatgaccg acttctttat gaatcaagat
      721 gcacaattaa caccggctca aaaaatcttt ctccattatt ttcctttggt ggaaatcaaa
      781 aaaatacagg agatctattc caataagttg ttgaaacaag aagatgatat ttataaaaaa
      841 caaatcctac tttttacatt gatctggatc cagcgaatca ggaaacatga atttatctct
      901 gccattaatt ggaaaaatac agaaataaaa gacagtagct tttctgcatt tattgaccac
      961 atgatcgagg aatttcaatt ggacaatgtc ccgcaagtag aaagaaatta tcttcgtttc
     1021 actattgaga cgttcaatgc tagagatatc tccaattcaa tcgagtgggt acaagctcaa
     1081 ttactaacga tccaattgat ccaatttgta gagaacgaga cacacgtacc tttccatcta
     1141 aaggaagaag ctttattcga aaatctctat aagcacatgg ctgcactgat tgtaagaatc
     1201 aagaatcata tccaagtcat gaatccattg aaagaaaaca tccgaacaaa ttatttccca
     1261 atctaccgcg cagtcactca ttttgttcct acgatcgaag aagtaattgg tggcaagata
     1321 ctggaggacg agatagcttt tcttgtcatc cacttttcga cgatcgccag tgtaatcaag
     1381 cgagacttga attatgttta taaatcagtc gttgtctgta accatgggat ggctactgga
     1441 aatctgttag cagagaatct gaaagaaaag tttcctcaga tcgagattac tgcagtttta
     1501 agttcaaaag aaatcgaatt agtagataaa ttggatgtca atctggtttt cagcaccttt
     1561 caattgaatt atcgaaataa gccgctttta gttgtcgatc cgattttaac tgagacgaat
     1621 atcttggtta tagaagactt tttgaaaaga aatgaagaac tacagcggtt agagcctaac
     1681 agtcatgaat cgacggagtt attcactcaa ataatagatg tcatgaaaga aagtggtggc
     1741 cgagtagatc gaatcatcta tggcaagtta gaaaaattat tcgaaacaaa caatttagaa
     1801 atcaacaaaa gggagatcca gcctatgtta aaagatattt tgacggacag caatatcatg
     1861 atcaaagaaa aagccactac ttgggaggaa tcgattgaac tagctgctag accattggtt
     1921 aaagaaaaca ttgtcgaaga gcgctatgtc gaagcgatga ttcgtgcagt gaatgattac
     1981 ggaccgtata tcgtgattgg caaacacatg gcactagccc atgcccgacc agaagatggg
     2041 gtcaataaat taggagtcag cgtagctacg attgagcaac caatcgattt cggtaatcct
     2101 gaaatggatc cagtaaagat tattttttgt ttggcagcag tagactccta ctcgcatctg
     2161 aacatcatga aagaactaat tgaattgatc aacaacgaag agaagttgga tcgattgatt
     2221 gaatgcacaa atatcgaaga tttcaaacaa ctgctttttt cagtagttga gaaataataa
     2281 atgatacggg ggagaacaaa gatgaacaaa ttgaacattt tattcgtatg cggtgcaggt
     2341 ttaggaagta gttttgcagc acagatgtct gctgaggatg ttttgaacaa attaggagta
     2401 gaagccaaac tggaccattg tgatatttct tccgcagttt ccatgaaacc agatgtgatc
     2461 atcacagcag aaaattttcg ttcgcagttc gaaaagttcg cagttgatcc taaaacggcg
     2521 atgatctatt tgaaaaatat tgtttcaaaa aatgaaattg aagaaaaaat caccccagtt
     2581 ctgcaagata agggagttat ctaaatacaa aaagaaaaga gggtaaaaac atgggcgtta
     2641 tcaattttat catcgaaaat attttgactc aggcatcgat caccattgca ttgattgcta
     2701 tgttgggtct actactgcag aagaaatcag caggacaagt catctctggc acgttaaaga
     2761 cactcctagg atttcaagtg ttgagtgccg gttcgagcat catcgttgga agtttaactt
     2821 atttcgggaa aatctttact gaaggatttc atatgcaagg gatcatccct tctattgaat
     2881 ctatcaatgg tcaagcaatg aatgatttgg gtttaggtag agatattgca ctgacgttct
     2941 tagccatttt tgtattcaat attattttgg ctcgtttcac aaaatggaag tacatttttt
     3001 taactggtca agcaatttta tggatggcaa cgatgacgac agtctttggc tattttgccg
     3061 gtctgcgagg gatcgtattg atcttggtcg gtgattttat cggagcttgt ttcgccattg
     3121 ctatgccggc agtagcgcaa ccgatcattc gtaaaatcac tggatcaaat gatattgctt
     3181 taggtcattt ttgtacgatc gggtacttat ttgaagccgg tgttgctaag ctttttggtg
     3241 agaaaggcga aaataaaaag tcgatcgaag atatcaagtt gccgacacat ttcgaatttc
     3301 tgcaagatac ttatttgtct gtcatggtcg tgatggtccc gctatatatc atcactgttt
     3361 tatttgcagg cgaaccattc gcttctgaat tatcaggaga tcaaaactat atcatgtttg
     3421 cctttttaca agccatccaa ttcgtagtgg gtgtgtatgt attgctagct ggtgtacgtc
     3481 tactgttagg tgaaatcgtg ccggcatttc gagggatagc tatgaaatta gttcctgatg
     3541 cgatccctgc cttagattgt ccagttttct ttccttatag cccaaatgct gtcattttag
     3601 gtttcattac gacaacgatt ggtacgatta ttgccatgtt tactttgccc atgttcggct
     3661 tagcgatgat tttacctggt atgctaacca atttctttgc cggagggaca gcagggatat
     3721 ttggcaacgc agttggcgga cgccgaggag cgatcattgg cggtattgct catggtttct
     3781 ttattacatt attgcccgct ttgctagtaa cgatctttaa ttcgatggga ttcatcaatg
     3841 ctacagcaac cgatgtagat acagtagccg cagcactctt atatgcctgg atcttaagcc
     3901 cagttttgaa agcattttaa ttgaaagtag ggaaaatgat gtttggattt tttaagaaaa
     3961 aagaaaaaga accgaaaaaa gttctggcaa aagaagaaaa agccctatcg ccagaagcga
     4021 ccgaaaagat tcaatcagag attacacaat tgaaacaaga gatttccact acccaagata
     4081 agcataaatt agcgaaactt tatgaacaag ttggacttaa attttctgaa ttgtatgtga
     4141 acgatcaagc catccaatat ttagaaaaga gtttggaaaa taaacagacg atcggagatg
     4201 ggtataaaaa actgatgagc ctctataacc agaagcgtgc agatgctgct cgtgctggtg
     4261 atgatcaagg gatcgattac tacatgggca aaatggatga gatgcgacaa attgcaaaac
     4321 aagtaacgat caaaggaaac aaataaaaag ataaggtggc aataaaagat gtatacaaca
     4381 ttgaaagaag taacaaaaac agcagaagag ttgaatttta cagttggagc ttttaatacg
     4441 cataacctag aaatgcttcc tgctatgcta ggcgcagcaa aagaaatggg ttcgccgatc
     4501 attattcaaa caagtgtcga taccgctaaa tatatcggca tgcatgtgtt agtcaatgta
     4561 atgacagcta ttgcagaaga agaaatggtt gatgcagtct tgcacttaga ccacgcccga
     4621 gactttgatg atatcaaaga agcaatcgac agcggatata cttctgtcat gtatgatggg
     4681 tcccatctgc ctttcaaaga aaatatcctg aagacaaaag cagtagtaga atatgcccat
     4741 gcgcacggtg tttccgtaga aggagaactt ggaacaatcg gcggtacaga agaaggaatc
     4801 catgtcgctg aaaatgacaa ggtatataca agaccggaag acgcagtgaa atttgtagaa
     4861 gctacaggcg tggatgcatt agctattgct atcggtacga atcatggaca attcaaatca
     4921 aaaacagaag taaacattcc cttgctgaaa gaaatcgatg cagtagttga tgtcccccta
     4981 gttatccatg gaggaacagg tgtgaaagaa gaagattatc ctgaattgat caataacggg
     5041 atccgcaaat tcaatgttgg aacggagctt ttagtcaact ggacgaaagt agctaaagaa
     5101 acctttagtg aaacagaaat caacaagtct cttcgccata atgtgattcc agcaaatgaa
     5161 gcagtaaaag aaattgtcaa gcataagatt ggactgttta tgaacttgga aagtccaatt
     5221 aacttaggaa ataaatgaga tgaaaaagta tttcattctt ctggctggta gtcctggtac
     5281 aggaaaaaca tatcttatga ataaactacg ggaacgattt ccagatatgt atgccatcac
     5341 gctcgatgaa atcaaggaat actatgcaga atccatcggg tttaacaatt tggaagaacg
     5401 ggcggaacaa gaaaggacga aagtttatcc tttctattac aaagcacttg atttgtatat
     5461 ggaagcagga aagaaagtga ttgtgtccga gtatcctttt agtgataagc aaaaagcacg
     5521 actccaagaa ttagcagata gatacggata tcaggtgatc acgattcgcc taacagcaga
     5581 ctttgaagtg ttatggcaac gtcggtacaa acgtgatcgt gaatcagatc gtcatttgag
     5641 ctatatcatg gatcattatc actatggtga cgtattggaa gatcgaacga aagggaccaa
     5701 tcatattacg aaagaagcat tcaaaaagat catcgccgac cgaaaatatg acaaattcca
     5761 gttaggaaca ctatatgaat ttgatgtaac agactatact caagtggatt acactccact
     5821 attggataaa ttagaaaaag aaatcaatga caataagtaa caaagaaatg gcttaatatc
     5881 gaaaaattgg cttttgaacg ctgtttaccg agaggtcgtg agcctgtcgt tcagctccga
     5941 gtcacaagga gcaatttc
//
LOCUS       DACENJ010000152         5931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.156, whole genome shotgun sequence.
ACCESSION   DACENJ010000152 DACENJ010000000
VERSION     DACENJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.156"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(143..628)
                     /locus_tag="IW748_002076"
     CDS             complement(143..628)
                     /locus_tag="IW748_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104982.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(767..1012)
                     /locus_tag="IW748_002077"
     CDS             complement(767..1012)
                     /locus_tag="IW748_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1104983.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1205..2005
                     /locus_tag="IW748_002078"
     CDS             1205..2005
                     /locus_tag="IW748_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1104984.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2024..3892
                     /locus_tag="IW748_002079"
     CDS             2024..3892
                     /locus_tag="IW748_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1104985.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3885..4376
                     /locus_tag="IW748_002080"
     CDS             3885..4376
                     /locus_tag="IW748_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1104986.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4364..4918
                     /gene="srlA"
                     /locus_tag="IW748_002081"
     CDS             4364..4918
                     /gene="srlA"
                     /locus_tag="IW748_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1104987.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4936..5931
                     /locus_tag="IW748_002082"
     CDS             4936..5931
                     /locus_tag="IW748_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1104988.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2073 a          947 c         1197 g         1714 t
ORIGIN      
        1 agtgagtata ttcataagag aaatagctgt aacgaattat taggttaatg caaatgctta
       61 tttatactcc gtataacttt ctcagaaaaa atgataccaa taatgacttg ctagtactgt
      121 aaatccgtat aatatcatct aatcaaaatg gtgttccatt tgcatctgtc atcgtaatcg
      181 atccttcggg tgacactcga taaaaaccaa cgcttccaga tcctccttgg gaacggattt
      241 gtttgctgga agctgtgaac aaataatctc gatcaatttt ttgataaaag ctaataacaa
      301 tatcgtcact ttgtaattcg gaacggtggg caataagaaa tagaagtgct ttgtcagagt
      361 tttctaatgg aagagaaggt acagtgcttt cttgcatttc agatgttgaa gaaatgctaa
      421 acgactgaat acttgattgg ctatgttgcg aatctacatt accagtagaa gtattagtaa
      481 tggaagtagt agaagtactt ttagttgaac caacctcagt tgttgaaatg gctgctgaag
      541 attctttaac agaattcttt gttttatatc cacaggcaga aaaaagtatt acagttccca
      601 acaagcctgc gtaaatccct ttatgcatga tagctcctcc taaaatagat tatctttatt
      661 cagtatatca cttaaacaaa gaaaagcaga tggtttaaaa tgaaaaaacc ttcccaataa
      721 agggaaggtt catgaagata ataacttttt taataattat agctttctaa tatctttcat
      781 caacttttct agtaatcatt gggaaaaatt tcaagcagta gatcgcacaa atacctacga
      841 cgatataaat gatcttcgca aaaatagttg ttgaaccacc agcaatcata gctactaaat
      901 caagttcgaa caagcctacc aacagccagt tcaaccctcc cacaatcaat aaagttaatg
      961 caatagcatc taagttttca ttagaattct cctttcttaa atgataaaac aattatatat
     1021 caaaaagaga aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat
     1081 gaaaattttt tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg
     1141 tcttaatttt ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc
     1201 acatatgtcg gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc
     1261 aggaattgga agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt
     1321 tgatgtaact gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc
     1381 ttctaaaacg gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga
     1441 tggtctagtc aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc
     1501 aaaaagcaag tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa
     1561 aagtgtatat ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt
     1621 gatcgtgaat ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc
     1681 tgctacaaaa gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag
     1741 gaatgttcgt gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca
     1801 agaatatgaa gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg
     1861 ttacagtcga acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga
     1921 tctagtctgc tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat
     1981 ttcaggaggt aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag
     2041 atggtaccaa ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca
     2101 aaaagaaatc aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga
     2161 aattgccggt attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga
     2221 ttttgatttt tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc
     2281 ggcatccaaa agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg
     2341 tatggatgat ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa
     2401 gacattaaaa gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg
     2461 actaagaata ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc
     2521 ctacttttca gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt
     2581 tcagaagaag acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat
     2641 ttctttgaat agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg
     2701 gtgttttgaa tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga
     2761 actaactttg agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac
     2821 aagtataacg atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat
     2881 gatgcgtatt cgacgattag atctcattga tctggatgag tccttcttat atcaagagat
     2941 gaaacagcac ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt
     3001 attttatggt gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat
     3061 acaagcatta gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac
     3121 gttgtatttg gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc
     3181 cattgttcat acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat
     3241 attggggagg gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct
     3301 aaacaaatat ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc
     3361 ggtgatccat ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc
     3421 aaaaaaaaag cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga
     3481 aaaaaagacg tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta
     3541 tgtggatgaa gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt
     3601 taacaaagaa tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc
     3661 agtagggcta ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt
     3721 atttgccata cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga
     3781 tatcctattt gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca
     3841 agaatcattt ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc
     3901 tattcatagt tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac
     3961 taaagcattt caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta
     4021 aacgacctgg gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc
     4081 aagttttaga agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac
     4141 ctgtctatgc gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg
     4201 aagatcgttt gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg
     4261 caggaaatga aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc
     4321 ggatatggaa aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa
     4381 atttgctgag ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat
     4441 gacaggaatc gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt
     4501 tttaggtgaa gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg
     4561 ttaccttgtg ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat
     4621 ggctcgtttc cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg
     4681 tcatacgagc aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg
     4741 gattgctcaa gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt
     4801 gcttgtggga ctgctgatga actttattgg tggttggata actgatttta ctactggatt
     4861 tgtatgcaga cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag
     4921 aaaggagagt tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga
     4981 ggtcctttga ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga
     5041 ggtgagaggc caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat
     5101 gggttcaaaa catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg
     5161 actcttagat gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca
     5221 ggaaaaagtg gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg
     5281 ttagaacaaa tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca
     5341 tctccagata aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag
     5401 cagcaaaact ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat
     5461 caatcagcaa gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc
     5521 gtttcccttt tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt
     5581 atggcacctt tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg
     5641 ccatttttat ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg
     5701 atcggtgtag agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt
     5761 gctatcaata ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca
     5821 aaagctgaga cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc
     5881 atcccacgag ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DACENJ010000153         5869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.157, whole genome shotgun sequence.
ACCESSION   DACENJ010000153 DACENJ010000000
VERSION     DACENJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.157"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            641..1375
                     /locus_tag="IW748_002083"
     CDS             641..1375
                     /locus_tag="IW748_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1104989.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IW748_002084"
     CDS             1391..2560
                     /locus_tag="IW748_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1104990.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IW748_002085"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IW748_002085"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1104991.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYAEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IW748_002086"
     CDS             3650..4576
                     /locus_tag="IW748_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1104992.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IW748_002087"
     CDS             4595..5845
                     /locus_tag="IW748_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1104993.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1964 a          956 c         1201 g         1748 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacaac aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgcagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgt
//
LOCUS       DACENJ010000154         5786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.158, whole genome shotgun sequence.
ACCESSION   DACENJ010000154 DACENJ010000000
VERSION     DACENJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.158"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(199..1890)
                     /locus_tag="IW748_002088"
     CDS             complement(199..1890)
                     /locus_tag="IW748_002088"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1104994.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(2310..3257)
                     /gene="arcC"
                     /locus_tag="IW748_002089"
     CDS             complement(2310..3257)
                     /gene="arcC"
                     /locus_tag="IW748_002089"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1104995.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(3372..4391)
                     /gene="argF"
                     /locus_tag="IW748_002090"
     CDS             complement(3372..4391)
                     /gene="argF"
                     /locus_tag="IW748_002090"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1104996.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(4482..5711)
                     /gene="arcA"
                     /locus_tag="IW748_002091"
     CDS             complement(4482..5711)
                     /gene="arcA"
                     /locus_tag="IW748_002091"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1104997.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         1539 a         1242 c         1021 g         1984 t
ORIGIN      
        1 agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac agtgtttaag
       61 agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac
      121 gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc agaacgctga
      181 tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca aacgaaggtc
      241 ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt tttgttcatc
      301 atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta atttgattgc
      361 atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg tttcagggta
      421 tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat agtctgctgt
      481 ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat gtgtatattg
      541 cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa agtcaaatgt
      601 attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc
      661 tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt tagcagattg
      721 gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag ttgataattt
      781 tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag accagtcaaa
      841 gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt cattccctac
      901 tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag cagctaagtc
      961 acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat tcaggtcata
     1021 agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa
     1081 aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca acgaatcgaa
     1141 acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat cacggaacca
     1201 ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc aagcacgagc
     1261 ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt ataaacgaag
     1321 caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt tgtaagcgac
     1381 gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga ttggctgata
     1441 accgattttt tccataatga aaccgatcga attaccgata actgtcgaac gcaaatgtcc
     1501 cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg ttccttgatt
     1561 cccgatgttg ctatccccat aatgttcttt ttctttcact acggtttgca aaactttttt
     1621 gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt cgattttttc
     1681 aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg gcgcttttcg
     1741 gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag ctgattttgg
     1801 attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag cgtcatagac
     1861 agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt tcccttgatt
     1921 gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta gagacgagat
     1981 ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga tatcggtaga
     2041 cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc ttcattcggc
     2101 ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg ttcaacaaat
     2161 cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg ctagtcgttt
     2221 cataaaaaaa aaatccagat ggcctaaagg agaagaaata ccacctggat agctataaat
     2281 catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt tccttcttca
     2341 gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc gtttggtttt
     2401 gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg agcgaattgc
     2461 ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt cttttgatct
     2521 ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag caaatccgca
     2581 tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc ttctcgtcct
     2641 tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga gactgtgata
     2701 actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat gggtttaggg
     2761 gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac agtggagtca
     2821 gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg tttacttgga
     2881 tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga aaccacatct
     2941 ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca gtaaccgata
     3001 gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt ctcagaatca
     3061 gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg ggagatgatc
     3121 aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat taacgcttct
     3181 tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa agcgacaact
     3241 acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt tctagataac
     3301 aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc gtgagatgaa
     3361 tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc catgatcgct
     3421 ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt gctgcggaag
     3481 acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa
     3541 acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg atctgtgttg
     3601 tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt gatacgttct
     3661 tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac gtctgctccg
     3721 tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt ttcagcaaag
     3781 ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc aacgatacgc
     3841 atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt gtttcgacca
     3901 tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg gatagttaaa
     3961 aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca aactggaaca
     4021 ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa ttcgattccg
     4081 tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt acctaattgg
     4141 atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc agtagtaaat
     4201 gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt tccttctaga
     4261 tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa
     4321 tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc ttggaatact
     4381 gattctttca tattaaacat tctcctttag attgttttta tttaagatta aagaggaaat
     4441 cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa gtcttcacgg
     4501 acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc acttgaattg
     4561 atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata atttcggtta
     4621 taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt ccattgttct
     4681 cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc atctaatcca
     4741 agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat atcatcacca
     4801 tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc tggatggata
     4861 gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat tgcacggttg
     4921 ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa taggtggcgt
     4981 gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc tactacttta
     5041 tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg atccaaccat
     5101 acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc tgtaaacatc
     5161 gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt tcccattgat
     5221 gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa tggatagtca
     5281 tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt tgtacgaacc
     5341 ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa ctcatgaaga
     5401 ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt caagaattgt
     5461 tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta aagagtctcg
     5521 acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc aatcggaaga
     5581 tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt ttccacttct
     5641 tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa aacgtgaata
     5701 ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat atcctccttt
     5761 tgtaatcact ttcataattt tgtctc
//
LOCUS       DACENJ010000155         5735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.159, whole genome shotgun sequence.
ACCESSION   DACENJ010000155 DACENJ010000000
VERSION     DACENJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.159"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..827
                     /locus_tag="IW748_002092"
     CDS             <1..827
                     /locus_tag="IW748_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104998.1"
                     /translation="KNKKINYSVDILLVDNPDKNKFETETKDLRTIGYTSSEKVLTNK
                     DMTNNQVNYSAVVRTVKERENNNPVKEFNESINYKFTDSTKTKTGYGISVDLEPTYRN
                     EIGKQIDMKVYSYPMNELIDSKIDEIYQSGGKETEVKMDRLKNDGKVESGISTQNLSF
                     QFPHMNIERKTGNIYADSQRNDESIKYELIDGGRKLYVPIWCDLGNYSIAIKSSNFGG
                     NYISLDMNQNIDVYAYMYATIDSETKKDDELLLEPVYPESTLPDGWSKNEIDWLNK"
     gene            887..1249
                     /locus_tag="IW748_002093"
     CDS             887..1249
                     /locus_tag="IW748_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1104999.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSIVFTDMVHKMLNGEPINFNFDYMLEKAQSNQIVAFIVSIYYFIEKSIFSLKDF
                     FFEMLTKLTTFYNKISKN"
     gene            1483..1905
                     /locus_tag="IW748_002094"
     CDS             1483..1905
                     /locus_tag="IW748_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105000.1"
                     /translation="MIYVAFFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETAD
                     YKFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            1917..3332
                     /gene="cpaF"
                     /locus_tag="IW748_002095"
     CDS             1917..3332
                     /gene="cpaF"
                     /locus_tag="IW748_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1105001.1"
                     /translation="MRVVEEKKISPKIEETPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSASEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            3349..4164
                     /locus_tag="IW748_002096"
     CDS             3349..4164
                     /locus_tag="IW748_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105002.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYLNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            4164..4961
                     /locus_tag="IW748_002097"
     CDS             4164..4961
                     /locus_tag="IW748_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105003.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKKDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIRPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            4979..5341
                     /locus_tag="IW748_002098"
     CDS             4979..5341
                     /locus_tag="IW748_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HAQ1105004.1"
                     /translation="MQEQSVMEIIKWFIGLMMIMAMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDRRFTIESDKLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVTLSFNGNSVSQIR"
     gene            5513..5734
                     /locus_tag="IW748_002099"
     CDS             5513..5734
                     /locus_tag="IW748_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105005.1"
                     /translation="MEAIQENRKDNQETSEKNKKKRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
BASE COUNT         2124 a          708 c         1102 g         1801 t
ORIGIN      
        1 caaagaataa aaaaatcaat tatagtgttg atattctttt agtggataat ccagataaaa
       61 acaagttcga aactgaaact aaagatttaa gaacgatcgg ttatacttct tctgaaaaag
      121 ttttaacaaa caaagatatg acaaataacc aagtcaacta ttcagcagta gttagaacag
      181 tgaaagaaag agagaataat aatccggtca aagagttcaa tgagagtatc aattataaat
      241 ttactgatag cactaaaaca aaaacaggat atggtatatc agttgattta gagccaacat
      301 atagaaatga aattgggaaa caaatagata tgaaagtcta tagttatccg atgaatgaat
      361 tgattgacag caaaattgac gaaatctatc aaagtggggg aaaagaaaca gaagttaaga
      421 tggatcgctt aaagaatgat ggtaaagttg agtcgggtat tagtactcaa aatctttcat
      481 tccaatttcc acatatgaat atcgaaagaa aaactggaaa tatatatgct gacagtcaaa
      541 gaaatgatga atctattaag tatgaattga tcgatggcgg tagaaaatta tatgtgccta
      601 tttggtgtga tttgggaaat tattctattg ctatcaaaag tagtaacttt ggtggtaact
      661 atatctcgtt ggatatgaac cagaacatag atgtttatgc ttatatgtat gcaacgatag
      721 atagtgaaac aaaaaaagat gatgaattac tgcttgaacc tgtttatcca gagtctactc
      781 taccagatgg ttggagcaaa aatgagattg attggctgaa taaataagtt tgaaaagttt
      841 ttatctatct gatagaatta aataatgata tagggatggt gatagaatga gggttcaaag
      901 ctatggtaac agtgtctttg atgttaaaag agaatttcaa gattctagaa gaaaaaccaa
      961 aaagctgata gcttacttca ttctatcagc tatcgttttc tatattgtca agtttgtttt
     1021 ggaattaaac tcaatagtat tcacagatat ggttcataaa atgttaaatg gagaacctat
     1081 taatttcaac tttgattaca tgcttgaaaa agcacaaagc aatcaaattg tggcttttat
     1141 cgtgagtatc tattatttta ttgaaaaatc tattttttct ttaaaagatt ttttctttga
     1201 aatgcttacc aaactaacaa cgttttacaa taaaatttca aaaaattaag gaggaagttt
     1261 gaatgttaga tcaaaataca gacagaagtt ggtggatgat tggtgccgtg atcgtaggtg
     1321 ccatcttaat cgggacagct aaagtagctt ttcccgaagt gtttaatatc gtcattacat
     1381 ttttcaaaaa catgatccct acaaacttag gatagttcca ctaaaaaatt cacgtagttt
     1441 gatgcgtaga aaaataataa agaatgaaaa aggctacata aagtgattta tgtagccttt
     1501 tttttggatg gaagtgatgt aatgctaaga gatatgccca aaatgattct tttaatgttt
     1561 gttgggttct ttttaatttt agtgattgcg tactattcat taaatggcta tacacaagat
     1621 tcgtctgtga aaggtatgca agaaacgtta agaactacag caatagcaaa cagggatgat
     1681 agtattcgag tagaaaaagg acgatttgct ttaattaagt ctgattttga aaaagatttt
     1741 aaagaaaaat ttgggaaaag taaaaatcta aataatgaaa ccgctgatta taaatttgat
     1801 tatcttacag atgggaagga cggtattaaa gcaattaaag tgaaggttga aagtggatca
     1861 aaagaatatc aagcaacttg tattttaaat gttgcaaatg attagaggtg tgaaatttgc
     1921 gtgttgtaga ggaaaagaag atttctccaa aaatcgagga aactcctaaa agtaaaatta
     1981 atatcgcaag tattattaaa aaaaatggaa ttaaagaaaa ctctgaagcg atagaaggaa
     2041 accttgattt tttaactaag cttttagaag aagatagtgg aaattatgaa ccaccaattg
     2101 aagatgcaaa attaaatatg attcgtgatg atttaaccag aaactattcc caagaagttt
     2161 tacaagcttt aactgatgat gaaattaggc gaaaacttgt acaagttgtt ttgaaagaac
     2221 atggtacttt ctttaaaaaa gataaagagg ttgcagaata tgtagtacgt gaatgtattg
     2281 gaacagggat tattgagcga attatgacca acgatgaaat tacagatgtt ggttggaatg
     2341 gctctcaact tagcattgaa actaactcta acaaatggtt gtttgacggt gataaattaa
     2401 atatcacgca agagtatatt gaacgtgtta tttctaaata tgctgttgta aatgatcggg
     2461 cttttaatag tggtaatcct atcatggatg gaatgtttaa aaatgttcgt atatcagcta
     2521 cacacataga taattcacca gatggctcta cgttttctat gcgtgtggca aggcctagat
     2581 tagcattgaa taaagaaaac tttgaagatt ttgctcctga ttatattttg gagctatttg
     2641 agaaaatgat gaaaacaaaa gcaaatatct gtatatcagg ggagacaggt acaggtaaaa
     2701 cggaacttca aaaattactg ctaagtttta ttgataatca tgatcgtatg attatgattg
     2761 aggacgttca agaaactcac gctaaagaat tatttccgga taaagatatt ttctcttgga
     2821 tcactactcc caaaaaagca atttcagatt tagtaaaagc ttctcttaga aataatccac
     2881 gatggatcat tgtttcagaa acacgtggtt ctgaagctta tgaaatgctt caagcggttt
     2941 tgtcaggtca ctatgttgta acgacacttc atgcggttaa tgcacgtgcg atacctagac
     3001 gttttgtaaa tatgtgtgca agtggtttta aaataaacga aaatgcggtt gaggaagaca
     3061 taaatcgtta tttcgatttt ggtgtccata tcaaaaaaac agtggttgat ggtaaagtga
     3121 tccgttattt gaatgaaatt gttgaatttt ctgctagtga accgactaca gtttttaagc
     3181 aaaaatttat caatggaaag tttgtcattg aaaatggtag tctttccgaa gattttaaag
     3241 aaaaaatggc tgaaaaattt attacgtttg aatttcctga agatcaaact aatacagaag
     3301 aatcacttcc tgaagaagaa gaggtgattt aagttgaaag tacaggctat gacaaagcga
     3361 aaaggtctca tggatgtttc ggaactaaac gcctatcaag tagcgtatgg taaaccgtta
     3421 gattatagag attatttgaa ctatgttgga ttaccttgtt tgtttatggg tggcttttct
     3481 tttgctttat gttactactg gtggattgct ttgatttgtg gagtaattgg aggcgtatat
     3541 ggctttaaag ttataatgcc taaaagtatt aaacgctctt atgaattaaa gagtttacag
     3601 gaaagaaata aatttattaa caacatgact caaattttga ccgatgatag taaaacagtt
     3661 actagagcgt tatctatagc aaaacagcga acaaaaggtg aattaagaga agatattcaa
     3721 atcttagaat cacgtttaca aggtgctgat aaatttcaga ttgctgaagc ttttcgtgaa
     3781 atcaacaata aatattatag tgatgttgtt tttacacaat actttgagca attggaaaca
     3841 gctatttacg aaggtagaaa taattcagat actatgaagc aaataaagag ctatcataat
     3901 gatgtgaaac gtaaaacaga aggttttatg aaaatcaaag acggctatct tcaagatatg
     3961 aagcaaatga tgtttattgt agctgttttt attggagcta taacttttgc ttttggtttt
     4021 tctactttct atgaaggttt tgctcgatca cctatcggat gggtatttgg atctatttac
     4081 tttttgatta tggttagctt catgaagtca tttttcaaac attattttga tgatgaaata
     4141 atgagtttgg gggtaagtaa ttaatgaaag ttaaggcttt aaaaaagaaa agaaaatcaa
     4201 gtattagccc cttgaaaatt acgaaaacag aacataaaag cttttggatt aaattactag
     4261 gtgatgttta ttatcaaaaa ttacgtgaaa tgaacaattc caatattgcg attattaagt
     4321 ggcaaaaaca acggttaaaa aagtcattga tttttatcct agtcggattc ttaatgtatc
     4381 tatttttagg gaaagaatgg tattacttag ccggtggaat tgctacttca gtaggttttt
     4441 atgtttggca agcaaaatca atcaatggta tctataatca atttcggttt gaacgacact
     4501 tgcaatttag taaatttaca cggctattga tcccctatct gaaacaaaaa aaagatggcg
     4561 gaaatttata tggtgttttt gcaaaaatcg taaaaagact agattatgat accgataaaa
     4621 agctattaat gaagcttatg caagaaatga cagatcagcc taacgatatt agacctttta
     4681 cggaatatgc agaacagact tctggaacag acatgtccgt attatttatg tctacagttt
     4741 atgatattcg acaaggttcg gcagatttgg atgttattga agagttggat cgtttagctt
     4801 ctgaagagtt aatggccgga atcgattcaa tcattgacta taagtcgagg cgttttgtct
     4861 tctttcctac aaaaattgca atgacttcat ttattttagt tgtcggtttt gcagtaggcg
     4921 ttcttttcat gaacctaaaa gatattaact ttgggcaata aaggaaagga gaaagtcaat
     4981 gcaagaacaa tccgttatgg aaatcattaa atggtttata ggtttaatga tgattatggc
     5041 aatggtttct ataggacttt tttgtgttca attacaagat attaatactt tcaagcaaca
     5101 agtaaattat caaattgaac gaaaaggagg tctgacgaca gaagctgttg aagcaatcga
     5161 caaatattcg gctactaatt atgatagacg ttttacaatt gaaagcgata agttaaatca
     5221 gaaagtatct tatggagaag aaattgatta tacgatcaaa gcaactattc caattcaaat
     5281 tttaccgctt cctgatgtaa ctttatcgtt taacggaaat agtgtttctc aaataagatg
     5341 agaggaagga taaaatgcaa gagaataatg tttccagaat aatttatttg attggcggat
     5401 tgataatttt atccttagct attgccggtc ttgtagagca ttggccggaa ataaagttat
     5461 taataagaaa cttttacgac aataaaatga ttaaaatgta ggaggtatag atttggaagc
     5521 aattcaagaa aatcgtaagg ataatcaaga aacaagtgaa aaaaacaaaa aaaaaagaaa
     5581 aaagaaagta gataaattta cggaaatgac cgataaaatc attgaaaaga aaggacttaa
     5641 acctaaagac tttgaccgtc aagcaatcaa agaagcacga caaaaactgt atgatgattt
     5701 tgttaaagaa aacatatcac agcttttaga ctaac
//
LOCUS       DACENJ010000156         5668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.160, whole genome shotgun sequence.
ACCESSION   DACENJ010000156 DACENJ010000000
VERSION     DACENJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.160"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1982)
                     /gene="copB"
                     /locus_tag="IW748_002100"
     CDS             complement(<1..1982)
                     /gene="copB"
                     /locus_tag="IW748_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1105006.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLA"
     gene            complement(2002..4188)
                     /locus_tag="IW748_002101"
     CDS             complement(2002..4188)
                     /locus_tag="IW748_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1105007.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4188..4397)
                     /gene="copZ"
                     /locus_tag="IW748_002102"
     CDS             complement(4188..4397)
                     /gene="copZ"
                     /locus_tag="IW748_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1105008.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4410..4850)
                     /locus_tag="IW748_002103"
     CDS             complement(4410..4850)
                     /locus_tag="IW748_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1105009.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(4924..5463)
                     /locus_tag="IW748_002104"
     CDS             complement(4924..5463)
                     /locus_tag="IW748_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ1105010.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1460 a         1272 c          992 g         1944 t
ORIGIN      
        1 gctagatcaa gaaaatgcaa aatatctttt ggatcgctgt ttgtcaatac gacatccgca
       61 gaatcgattg cgatatctgt ccctgcaccg attgccatcc cgattgaagc acgggctaaa
      121 cttggcgcat cattgatccc atcacccacc atgattactt ttttcccttg gtcaaggtat
      181 ttctggacaa cagcttcttt atcatctggt aataaaccgc cataatattc ttcgatccct
      241 aaataatctg ctactgcttg ggcagcgttt ttattgtcac cagtaagcat gacaggagtg
      301 atttttcttt ctttcaatgt tcggataaat tcttttgctt ctgtttttac tacgtcacct
      361 aaagcaacga ttgcagctag atgattttct aaaatcagga agctgatggt atttccttgt
      421 tcttggtaag gtttcaataa tgcttgttct acgtttaatc cgagacgctc aacttctttt
      481 tcattaacta ttttcacttg ttgttttttg actgttgcct ctagcccgac tcctgaaaga
      541 ttttttagat tttcagcttg ataaggcttg atttcatgtt ctttcaaata attcataatg
      601 ccaacagcca atggatgatt ggcattcgct tccaaggctc ctagatattg aaggatttca
      661 tttttattga acgcatcatc aagtacttcg acacccgtta cagtaaattt tccttctgtt
      721 agtgttcctg ttttgtccag catgatcaca tctagatcat tggcttgctc cagtgcattg
      781 cggtttttca aaagtaatcc gtttttagct gctattgatg tggagcgagc aacaactaat
      841 gggatagcta aacctagagc atgaggacag gcgatgataa agactgttac catacgttct
      901 aatgcattag gaagatctgt caagaacaac caggcgatga atgccaggat acccacaatt
      961 aatgcgacat aaaacagcca ttttgccact ttgtcagata atgactctag cttggatttt
     1021 tctccttgtg cttttcgcac catttccata accttcgcta gataaccatt ttcaccagtt
     1081 ccagttactt cgatttcgat cgttccatcc ccattgatag aaccgccaat cactttgtca
     1141 ttggcttgtt ttttgattcc tcttgattcg ccagtcacag ctgattcgtc aacaattgtt
     1201 tcacctttca agattacacc atctgttggc attttatcac cagaccggac gatcaaacga
     1261 tcaccttcgt ttacttcttt caatgaaact gtctcttctt ttccatcttt cgtcaagcgt
     1321 ttcactgatt ctggcagtaa ttctgctagt ttttgtaaag cattgctggc attcgacaca
     1381 gcattcattt caatccagtg acctagtagc ataataacga tcaaagtagc caattcccag
     1441 aagaaatcca tgacgtgtgt atgtggattg agcagatttg cgataaacga atagacacta
     1501 taaatatatg caactgtaat ccccatcgcg atcagtgtca tcatagcagg acttttttgt
     1561 ttcaattcca tcttagctcc acttaaaaat ggctgaccgc catagataaa tagtatagtt
     1621 gctaagatca atacgagcca atcagaacca ggaaacgtta cttgaaatgg aaattccatt
     1681 cccatcattg gtgaaaaaag aatgattggg atagctagga taagcgatag ccaaaatttt
     1741 tgtttgaaat tccccatgtg catggaatga tccatgccgc cgtgttcgtg atccatgtgc
     1801 atcgaatggt ccatttgttt gctgtttgct atatctccat ggttatgtcc catgtgcatg
     1861 gaatgatcca tctgactatg gtcatgtttc atctccatct gtttttcatg agattctgta
     1921 ctttctttgt ttactacttc ttttttatgc tcatgttttt tctccatatc gttcccattc
     1981 atttttactc catcccttct cttacttttt catgtgtcga tttaagctca gggagttcaa
     2041 caatacactg acggaactaa acgccatcgc tcctcctgca atgattggat tcaaaaagcc
     2101 tagtgcggca aaaggaatac caatcgtatt atagacaaaa gcccagaaaa gattttgttt
     2161 gatttttctc aatgtcaacc tagacaactc gattgtttgt gcaatggatg ccaaactgct
     2221 attcatcagc gtcacatcgg ctgtttccat cgcaatgtcc gttccacttc ccattgccat
     2281 accgacatca gctaatgcta aggccggtgc atcattgatt ccatcgcctg ccataccgac
     2341 ttttttgcca tctttctgaa gtttttctac gtaggctgct ttatcctctg gaagtacttc
     2401 agcaaagata tgctttggat caataccaac ttgtttacca atcgtttcgg ctgccagttt
     2461 gttgtctcct gtcagcataa aaacatctaa tcctttattc tggagctgct tgattgcttg
     2521 tttggcttct agtttgattt gatcagccac ggcaatccac ccgatcactt gcttttcatc
     2581 agctaaaaac atgactgttt ttccctgctg ttctaattct aacgcgtgtt cttggtattc
     2641 atcaaatgat aggttcaatt caattaatcg tttacgtgta cccgcaaaat agcgtactcc
     2701 attaatcgta ccgctgatcc cagcacctgg gtgggctgta aaatcaatca tatcgtatgt
     2761 tgctgcttct tgcagttttc cgtattccac gattgcttta cctagtggat gttcagaagc
     2821 ttgttctaaa gtataaaaaa tagataggac ttcttttgaa ccgactagat ctgtaacttt
     2881 gggctttcct tctgtaatcg ttccagtttt atccaaaaca atgctgtcta agtgagcagc
     2941 tgcttctaat gcttctccgc ctttaattaa aattccattt cgtgcaccaa cacccgttcc
     3001 taccataata gctgttgggg tcgctaaacc taatgcacat ggacacgcaa tgactaatac
     3061 agagacacta tggatcaatg cgagttgcca atcaccagtg atcaaacctg tagctatgag
     3121 cgtgataaac gcgatgacta atacgatcgg aacaaaaatc cctgagatcc gatctgcgat
     3181 tttttggatt ggggctttgc tcccttgagc atcttccacc atttgaatga tttgcgctaa
     3241 tactgtctgc ttgccgactt gtgtgacttc catgtgtagc agaccattcg tattgattgt
     3301 cccgccaaaa agcgtgtcat ccggattctt ttctacagga agattttcac cagtaagcat
     3361 actttcatct atcgcactgc ttcctgaaat aattcgacca tctgctggaa tctgctcacc
     3421 aggacggatg actagttgat caccaacgac tacttcttca atcgctaacg tttcttcttt
     3481 cccgtttctt atcacttggg cggtttttgt ttgaagagac atcaattgtt tgattgcatt
     3541 acctgttttc gtttttgctg tatgttccaa atatttgccg agcaaaatca gcgtgatgat
     3601 catactgctg ctttcaaaat aaagatcttg ggggtgtcca cggaaaaatc cgttgtaaat
     3661 gctcaatgca aaggcagccg atgttccaat tgctaccaat acatccatat ttggtgcttt
     3721 cgtcttgatt gcatggtacg cacctttata aaaacgagca ccgacataaa attggacagg
     3781 agcagacagc actaactgga cgattggaaa atgaaaaaag tgaacaatcg ccgcatggct
     3841 tcctaacatc atagcaatca ttgacaacat caacggcaaa gtcaaaaccg tactcaatat
     3901 aagatcgaat agcatttttt tcaaatacgc ttgcttttct tctgcaattt tttgtttgtg
     3961 ggcttcatcg tacaaaattg caccataacc gatgttttct acgctttgga ttaatttttc
     4021 agtggtcgtc ccttcatatt tgacacttgc tttttcagtg gccaagttga ctgtggcatt
     4081 gaccacacca ggttgtccgt tcagctcttt ttcgatccta gcagaacaat ttgcacaagt
     4141 catacctgta atgacgaacg tatccatttt ggtttctgct gttgccatca aatcacctct
     4201 gctttataac ccaatccatt gattgcttga caaatctttt cggattgaac ttttgtttca
     4261 tcaaacttga ccgtaccgtt tgcttttttc aaatttactt tcactttttg cacgccgtca
     4321 agtgcgctta ctgtttcttc tactcttgct gcacaatgat tgcagctcat accttcgatt
     4381 gaaaattgtt gtttcatttt ttcttcctcc tattgatgtt gtttacattc acattgacct
     4441 gggatacagt tacattcgat cgtatctact ggttgctttt gttccagttg tttcaaaatc
     4501 tcattgatat cttcttgagt caaagtggct tcttctacta gatccgcaat cgtctctcca
     4561 atcctttttg cacagatatg ggaaaaaaga ttttccgtcg cactgcgaac tgtttccatc
     4621 tccgagacag ctggatgata aatgaatttt tttccttctt gttctgtcca gatcacttct
     4681 ttttttacca aacgtcccag caaagttttg atcgttgctg gtttccaatc catcgaatca
     4741 gctaagatct gcgtgatttc ttgggctgtc gtctgtccaa gtgtccaaat aacacgcatg
     4801 atctcccact ctgaatcact gatctttact ggcgttttga ctttttccat ttatttcaca
     4861 cctttcgttt acaaatgtaa tcattagctt atgaatacag attacgcttg taaacaaaaa
     4921 ctgtcaatta tttttgctct ctattttcaa tttagttaaa aattcttgtt tctctcgtct
     4981 tgttttaagg atgatcagtt tatttcttgc attagctact tggatcagct gctgtatttt
     5041 cggttcattg ttttcttcaa aatgccagat aaaagataaa aattctaagt attccctgtt
     5101 tagtttctct gaaaatgatt caggcatatc tggccgagat tttttgttta tctttctttt
     5161 tagagaacgc caaactactc ggtagatggc gagataacgc ggcactttaa gccaaatgat
     5221 cttatctgct tctttaatcc tttcctctaa tgtagaagta taatttcctt caacaatcca
     5281 tgacggatta gataccataa acgattgctg ttcttgcaaa aaccactttt ttgcctgaat
     5341 actgtagtct gttgtatgcc ataaaaggtc tagcggcagt agcggaaagt cggtgatttc
     5401 tttgatcttc cttgcgaggg tcgattttcc cgacccactt tgtccaatca cgatgatctt
     5461 cattcttctc cctcacttct ctttggaatt tggttcgatc ataccactta taacgccgca
     5521 aaaaaacaag ggactgtgac aaaagtcgtg tcaaagtccc ttgtttcgaa taaatggtgt
     5581 tcaatcagct gaccttcagc attaactcaa aaaacggcaa aatatgagga gctatttttt
     5641 cgtttgttcc tctaatactc aggtcaaa
//
LOCUS       DACENJ010000157         5650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.163, whole genome shotgun sequence.
ACCESSION   DACENJ010000157 DACENJ010000000
VERSION     DACENJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.163"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            58..537
                     /locus_tag="IW748_002105"
     CDS             58..537
                     /locus_tag="IW748_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1105011.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            756..1436
                     /locus_tag="IW748_002106"
     CDS             756..1436
                     /locus_tag="IW748_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1105012.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            1444..2775
                     /locus_tag="IW748_002107"
     CDS             1444..2775
                     /locus_tag="IW748_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1105013.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            2790..3752
                     /locus_tag="IW748_002108"
     CDS             2790..3752
                     /locus_tag="IW748_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1105014.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            3863..4201
                     /locus_tag="IW748_002109"
     CDS             3863..4201
                     /locus_tag="IW748_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1105015.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            4280..4987
                     /locus_tag="IW748_002110"
     CDS             4280..4987
                     /locus_tag="IW748_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1105016.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(5090..>5650)
                     /locus_tag="IW748_002111"
     CDS             complement(5090..>5650)
                     /locus_tag="IW748_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1105017.1"
                     /translation="TQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLCFPE
                     ATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQI
                     TKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNT
                     VLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         1851 a          976 c         1244 g         1579 t
ORIGIN      
        1 ttttgaatac agcatccctc gctttataat aaacgataga ataaagggag tgaatcaatg
       61 atgagtcaaa caaaagtcga acgttattta gaagaacaaa agatcagcta tcaaccactt
      121 gattttagtc atgtggtaga agaatctttc acagaagaac tacaaaaaag agggattgat
      181 ccagctttga tatgtaaaac tctcgtagtg aaaggaaaca aaactggggt gatcatcgca
      241 ttgattcctt tgaacgatca cttagattac aaaaaaacga gacaagtgac gaaagatcgt
      301 aaagttggtt ttcctggaat ggattttgta ttaatgcata caggatatcc ccacggagca
      361 aacaccccta ttggaataaa actcgcacat cctgattatt gttttttagc agacagttct
      421 ttaaaagaaa aagaagaagt aattgtgtca agcggtgaaa tcgggagaag ccttcggata
      481 aaagtagccg atttagaaaa gatcgtccat cctatttacg ctgatttaat tagttaaggg
      541 acgagaagag aagctaagca gagcagagac ctcaaacttg agattatatc tgcttggtac
      601 ttggtgcatc ctgtattatc tgttacaatt gctttgtaca tactgtacta tcaatccaat
      661 gaaaatatat ttagcagcta tattttttca ttgatgtgca agtgatgaaa tcgtttttaa
      721 taaaaaagat acagagaaaa cgcgaggtgt aaaatatgaa aattacgtat catggacatt
      781 cctgtttaac aattgaaatg gaaaatggcc aacgcgtgat gattgacccg tttattacag
      841 gaaatccatt gacagactta actgcagaag aagtaaagac agattggttg ctgattactc
      901 atggtcatag tgaccacata ggagatatga ttcctattgc aaaagaaaat caagccacaa
      961 ttatcagtat tgttgagatt gctgattatg cgaacagcag aggagtagat tcattcggta
     1021 tgaatatcgg tggaaaacat gcgtttccat tcggcgtggt aaaattcgta catgcacagc
     1081 atagttcaag ctatgaagta gacggtatcg ttcagtatat gggcgagcct agcggaatta
     1141 ttattcaagc agaaggaaaa acgatttatc atgcagggga tactgcttat ttcagtgatc
     1201 taggattgct tgcagaagaa ttcgatattg atgtcgcttt cttgccgatt ggcgataact
     1261 atacgatggg gccagaagat gcagcaaagg ctgcgcaaac aattcgtgcc aagaaagtag
     1321 ttccaatcca ttacaatact ttccctgtca ttgaacaaga tcccgaatct tttgtagagt
     1381 tactggaaga tagtgaagga aaaataatga gcgtctcgga aacgctggaa gtgtaggcag
     1441 aaaatggcga caaaacatga tttgatttta gaatatattg aaagtttacc tgttggcaat
     1501 cgaatttctg taagaagcat cgcaaaagaa ttgaatgtca gtgaaggaac agcgtaccgt
     1561 gcaatcaaag atgcagaaaa tgtcggacta gtttcaacaa ttcaacgagt agggaccatc
     1621 cgtatcgaac gcaaattaaa aaaacatatt gaacgtttga catttggcga ggtcgtcagg
     1681 atcattgaag gggacgtttt aggcggggcg gcaggattag acaaggtctt gaataaattc
     1741 gtcatcggcg cgatgactaa acatgccatg gaacgctata ttacgccagg atcactaatg
     1801 atcgtcggca accgggcgga agtccaaaaa ttagcattgg aagatggcgc cgcagtattg
     1861 atcacaggag gattcaatac gactgctgag attgcaaagc tagctgatga tcttgaaatg
     1921 ccgatcttac gcacctctta tgatacattt acagtcgcaa ccatgatcaa ccgagcgtta
     1981 agcgaccaat tgatcaaaaa agatatcatg cttgtcagtg atatctatat gccagctgaa
     2041 aagactcatt atttgcagca gacagatacg atttctgatt atcagcgttt gtcagaagaa
     2101 acgcagcatt cgcgttttcc agtcgtcaac cgccatcatc gtttgatggg gattgttacg
     2161 gcaaaagatg ttttagggaa atcgccaaac cagatcatcg atcgggtgat gacgaaagaa
     2221 ccaatcagtg taaagaaaac aatgagtatt gcctctgtta gccatcaaat gatctgggat
     2281 gggctagaag tcatgccggt cgtttccgat gacttgacat tggaaggctt gattacccgg
     2341 caagatgtaa tgaaagcgat gcagcttgtc cagcgacaac cgcagattgc agatacgatt
     2401 tcggatcaga tatctggaaa tatccatacg attgatactg accgtgaagg aaatcgtttg
     2461 gaaaacccta agttcaaatt cgtagtagca ccacaaatgg tcaatggcgt tggaaccatt
     2521 tcttttggcg tacttagcga aatcatttct gatgtcgcac aaaaaacaat ggtgatgaac
     2581 caaaaacgaa atatcttgat tgaacaagtg aacctacatt atttgcggct gatccaattg
     2641 gaaagcgaat tagacattcg tccacgtgta ttggaaatcg gcagacgttc cgctaaactg
     2701 gatatcgaag tgtttattga aaacaccatc gtttccaaag caatcgttgt ttgtcaagta
     2761 atggaacgtt cttagaaagc aggagtgaaa tgacagtaca gcaagaaata gtagatacaa
     2821 taaaaaaata cgataaaatc attatccaca ggcatttgcg ccctgatcct gatgcattag
     2881 gatcacaagt aggattagcc gaactattac gggaaagttt cccagaaaaa gaaatacgtc
     2941 aagtaggtga aacgattgaa gggcttcgct ttttagcaga aatgcaagaa gttgaagatg
     3001 tcttctacca aggagcgcta gtcatcgtga ctgatacagc taattcaccc agaatcagtg
     3061 atcaaagata tcagttggca gaaaaactga tcaaaatcga ccatcatccc aatgatgacc
     3121 cttatggaga tcttgtgtgg gtaaatacaa aagcaagtag ctgtagtgag atgattgcag
     3181 aatttgcttt gatgtttcct gaatctttaa agatgaacgt taaggctgca cggttacttt
     3241 atgcaggaat cgtcggagat acagggcgct ttttgtatcc ctccacgacg tcccgtacat
     3301 tagagattgc ttcgatattg agaaattatc catttgatgc aagtgctttg aacagagaga
     3361 ttgaacaaat gccgatgaaa gtcgctaaac tttccggtta tctttatcaa aatatccagg
     3421 tggatgaaaa tggtgcagga agaataatcc ttccgcaaga aattcttgat aaatatggaa
     3481 tcgatgattc agagacagct tcgatcgttt cacttcctgg agtgattgac gaagtgctgg
     3541 cttgggggat ttttgttcag cagccttcag ggtattaccg cgtacgttta cgttctaagg
     3601 ggccgatcat caatgaattg gctaaacgcc atcatggagg aggccatcca ttagcaagtg
     3661 gtgcgaatgc aaaagattta gcagaagtag aggaaatcta taaagagatc caagatatat
     3721 gccaatcgta taagagcaga aaaacagaat gatacttcgt tcgaaaacgt gccttttgta
     3781 gtaaaaatca caaatgtatc ttttcaagat cttctttttc cgttacaata gtgctcacta
     3841 aagattttaa aggagaaaga agatgacatt accaaattgc ccagaatgtg gctcagagta
     3901 cacatatgaa gatcgaggct tgttgatttg cccagaatgc gggaacgaat ggaacaaaga
     3961 agaacaagaa actgtcatag aagacggatt agtggtatta gatgcaaatg gaaaccgtct
     4021 gttagagggc gatagcgtga cagtgattaa agacttgaaa gtaaaaggtg cgtcaggtgc
     4081 cattaaacaa ggaacaaaag tcaagaacat ccgtttagta gaaggcgatc ataatatcga
     4141 ttgcaaggta gaaggatttg gaccgatgaa actgaaatcg gaatttgtga aaaagaatta
     4201 aagaatattc aaggtctttt cacattcttt tcctactact aatgataaaa taaagtaaac
     4261 gaaaaggagt gaaacgaaaa tgtatggtta tggaatgtat ggttttttag atccgacatt
     4321 tcttttggtc attcttggat tagttatttc cggaattgcg tcagcgtacg tcaatagtac
     4381 tttccgaaaa tacgatcaag tgagaagcaa aaacaatgtg actggtacac aagcagcaca
     4441 atatatatta caaagtcaac agataaataa tgtcggtgtt caacaaattg ccggagatct
     4501 cacagataac tacaattcag gtaataagat gttgagtttg tctcaagcaa ctgcacagtc
     4561 tacttcagtt gctgcgatag gtgtagcagc tcatgaatgc ggacatgcgg tacaagatgc
     4621 ttcgggttat gtcccattga agattcgtgc agcacttgtt ccagcagcta actttggttc
     4681 gatgatttcg ttccccttga ttatggtagg tgtattgctt agctggaact ctacgttaat
     4741 caatatcggg attttcgctt tttctttagc attacttttc caattggtga cattaccagt
     4801 cgaatttaac gcgtctcggc gagcaatcca aattttgagt gaaggcggac tactgacgga
     4861 agaagaagta ccgatggcaa gacacgtatt atttgcagca gcactgactt atgttgcagc
     4921 agcgctttcg acattcctgc agctgttgcg cttgatctta ttgttcggcg gcaaccgaag
     4981 agattaaaat aaagaagttg tgaaatcagc gctttgatct gagctaatac cgagagttag
     5041 ctgattgaat accatttatt tggaataaga ggatgtgaca agaattttgt cacatcctct
     5101 ttgttttgtt ctcataacct tttttcatat acagttcatg aaccgctttt attaacttgc
     5161 caaataacac tgtattgtgc gttgtatcca gatagaaagt ctccgtttca tctgaaattt
     5221 tctcatgctg atctgtaatg atcaaatcag catgttctga atatgcggta attttaacag
     5281 catctttgcg aaataactgc tgcaaatggc tttgaagatg ataatttcct gtaaactgtt
     5341 tggttatctg caaatggatg agtaactgct ctttcgtttc tgtttgtagt aagtgttgga
     5401 tcaattgact aaggtattga ctgatttggt ttctttcccc attttttgaa aaaattgtat
     5461 cgattttttc tcttaatttc ttttcctgta taaaagaact agaatgattg gacgtggctt
     5521 ctggaaagca aagtgtagaa aaactactgc tggattcacc caacagttcg aaaaaaacaa
     5581 ctgccaacac aagaaaataa aaataaattg gtattttatt ttctaaatgg ttgagaggaa
     5641 actgttgagt
//
LOCUS       DACENJ010000158         5638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.164, whole genome shotgun sequence.
ACCESSION   DACENJ010000158 DACENJ010000000
VERSION     DACENJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.164"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2386
                     /locus_tag="IW748_002112"
     CDS             <1..2386
                     /locus_tag="IW748_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1105018.1"
                     /translation="DLSESLGFIHENPHQADSDAEVTGQLLLLIEERMRKLPIITLEQ
                     IARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDGLALQKKEVELFTSVHYGER
                     TYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDESKDLMIEAATGMGKTIGYLL
                     PLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQPIQPVIIKSYRHYIDLQRF
                     KGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTNLNHSFFADIAHRGIGFLAR
                     NQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEPLLPASPFLLIDEVHHLPEI
                     AEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEHEALRWLSIYREELSALCEA
                     QICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLESGTAIDHLQLFYQEIQQLQS
                     QMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMDQWLEDWRDHLIHWFVPGNT
                     AQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGPNRHYLSKRLGIEAAPLKVI
                     ASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTGLIEQEDRPILVLFTSHDVL
                     QKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEKSVLFGADSFWEGVDLPGDA
                     LQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESVPKAALKLRQALGRLIRTET
                     DRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEIYQDIHEFLKKNEEGS"
     gene            2433..2960
                     /locus_tag="IW748_002113"
     CDS             2433..2960
                     /locus_tag="IW748_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1105019.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            2981..4171
                     /locus_tag="IW748_002114"
     CDS             2981..4171
                     /locus_tag="IW748_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1105020.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4211..5509
                     /gene="asnS"
                     /locus_tag="IW748_002115"
     CDS             4211..5509
                     /gene="asnS"
                     /locus_tag="IW748_002115"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1105021.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         1874 a         1044 c         1248 g         1472 t
ORIGIN      
        1 tgatttgtca gaaagccttg gatttattca tgagaatcct catcaggcag atagtgatgc
       61 cgaagtgacg ggtcaacttc ttttgctgat cgaagaacgg atgagaaaat tgccaatcat
      121 cacgttagag cagattgcgc gattaagtag acacacaggt atggatacga gtcgtttcat
      181 ttatcatgtg attgaagaaa tgaaagaaaa accagagcca cttgatccct cattagaaat
      241 cgtcgatggg ctagctttgc agaaaaaaga agtggaacta ttcacatccg tccattatgg
      301 cgagcggaca tatccaagaa aaaaacaagc aaaagaaaaa atgtttggta aaacactgat
      361 gtatcgaaaa gaacagaaca gactgatgaa tgcggtctac gaccatttca caaaagatga
      421 atcaaaagat ttgatgatcg aagcagctac aggaatgggt aaaacaatcg gttatctgct
      481 gccattgagt tatttggcta cacctgaaaa acccgctgtc atcagtaccg tttcgctagt
      541 tttgcagcag cagatcatag aaaaagacat tccattgctg aatcaattgc tagatcagcc
      601 catacaacct gtaattatca aaagctatcg ccactatatt gatttacagc gttttaaagg
      661 cacacttgtg gaaccgccag aacaaaaaca atatgctttg tatcaaatgg cggtcttagt
      721 ttggctgacg caaacgaaga ccggtgattt ggatgaatta catttaacaa atttgaacca
      781 ttcattcttt gcagatatcg cgcacagagg tattggattt ttagcaagaa atcagtcatt
      841 ttacgaacaa gactttgttc gtttcttgca aaagaaaatc aggcaaagta attttttgat
      901 tgtcaatcat gcgtttttaa tacaagaaac acagcgaaaa gaaccattgc ttccagctag
      961 tccatttttg ttgatcgatg aagtacatca tttgccggaa atcgctgaaa aagtaaatga
     1021 caaacgatta agtattaact atttccataa acaaatccag caattagaag aaccaaatca
     1081 gctgtttgac cgaatacaaa gctatttacc gaaagaacat gaagccttac gctggttatc
     1141 gatttatcga gaagaacttt ctgcgctatg cgaagcacag atttgtatgg cagaaggatt
     1201 aatgagttta gtgagcggca agcaacctaa cggacagtat ccagaagaag tcatgatgac
     1261 aaaagagatg ttggatcagc tgccactgga atctgggaca gccattgatc acctgcagct
     1321 tttttatcag gagatccagc agctacagtc acaaatgcgt ctgacttttg agattatcca
     1381 agaaagctgg acaaaccaga tgcggatcga tttagcaagt ttatatagct tatttgatca
     1441 attagacaag cagtacttgc tgatggatca atggctggaa gactggcgag atcatttgat
     1501 ccattggttc gttcctggga acacagcgca gcagatgatt tttcatctcc atgattttca
     1561 agcggctatg ttgccgtcga ctgtttggta tcctcgatat gatcgtattc tttatattgg
     1621 aggaacgctg aagatcggcc ctaatcgaca ctatctctct aagcggcttg gcattgaagc
     1681 agcacctctt aaagtgattg cctctcctta tgattatgag aaacaggcaa ggatctatgt
     1741 gccaaaagag gcacctgccg tccatcaagt cagcaatcaa gcatatatcg attatttaga
     1801 gacagcactt acaggtctga tcgaacaaga agatcggcca atacttgtgt tattcacttc
     1861 tcatgatgtt ttgcagaagg tctatcagaa aatgcatctg tctatgctag aaaaaggacg
     1921 agaaatacta gctcaagggt ttggcggaag ccgggagaag cttttgaaac gcttcatttt
     1981 gtcagaaaaa agcgtattgt ttggcgctga cagcttttgg gaaggtgtag atttgcctgg
     2041 ggatgctttg cagatcgttg tagtcacacg tttacccttc gataatccgc ttcgcccgat
     2101 ggtcaaagct aggaacgctt atttagaatc agaggggatc aatccttttt accaggaatc
     2161 cgtacctaaa gcagctttga agttacgcca agcattaggt cgcttgataa ggacggagac
     2221 agatcgtggc gtattgctta ttttggatcg aagactagtc actgcaaaat atggtgcacg
     2281 gatcatccat gcattaccga aaaaagtacc gatcaaagag ctagctatgg aggaaatcta
     2341 tcaggacatc catgaattct taaagaaaaa tgaagaagga agttagagga gctgtctttt
     2401 ttcataatta aaatctgcta taatgaaaag gaatgaagat aaaggagcga cgacccttga
     2461 agaaaaaaca gacaaaggaa aaaacgatta atcgcgtgtt acttggcttg atcgtatttt
     2521 tattagcggt catcgtgatc agctcgattt tttacttgcg ttccagtcgt ccggcggtgc
     2581 aggcgaagaa agaagcagta gaaatcgcaa agaaatatgc aaatcttgag gaagccgatc
     2641 atttttattg gtttactcga aagaagacat acttttcact gacaggtaca aatgataaag
     2701 gaaaagaaat cgccgttatc attcctaaat ctggtgaaaa agtaaaagta ttggatcaaa
     2761 caaagggact gactgaaaac gaagcaaaac agacaattgc tgcggcccat cctgaaatcc
     2821 aaatcgaaaa agctgcttta gggatgtatg atgatcagcc tgtgtgggaa gtagtaggaa
     2881 aagaaacaaa tgggaaactg aattattacc tgattggctt tgaatctgga gatgaagtca
     2941 agacaatcaa gggaatttaa aaggtaaagg aatgaatggg atggagttat caaaaagaac
     3001 aaaacgatta gaaccgtctg tcactttggc agctgcggca aaggccaagg cattaaaagc
     3061 aaaaggcaga gatgtgctga gtttgactgt gggagaaccg gattttgcga caccagaaaa
     3121 catccaagaa gcagcaatcg aagcaatccg caacgggaaa gctagctatt atacaccaac
     3181 agcaggaatc ccagagcttc gacaggcaat cgttgattat ctgaagaaaa actatggact
     3241 tgtctatgaa ccttcacaaa cagtcgtaac agacggagcg aaatttgctt tgtataccct
     3301 tttccaaacg atccttgacc cgcaagatga agtcatcatt cctgtgcctt actgggtcag
     3361 ttacggtgag caagtaaaac tggcagaagg acttcctgtc tttgtgaaag gggaagaatc
     3421 caatagctgg aaagttactg ttgaacagct agaacaagca cgttcagaaa aaacaaaagc
     3481 cgtaatcatc aattctccat cgaatccgac tggaatgatt tatagcaaga atgaactgca
     3541 ggcaatcggt gaatgggcag taaaaaatga tattttgata gttgctgatg atatttatgg
     3601 tcgtttagtt tataatggca atgagtttac tccaattgca acgatttcag aagcaatcaa
     3661 aaatcagact atcatcatca acggcgtgtc taaaacatat gcgatgacag gctggcgcat
     3721 tggttatgcg gtgggaaatc ctgtgatcat caatggaatg atcgctgttg cttcccagtc
     3781 aaccagtaat ccgacagcag tcagtcaata tgcagctgtt gaagcattga aaggcgagca
     3841 agatacagtg gaagaaatgc ggaacgcctt tgaagaacgc ctgaatacgt tatattcgct
     3901 tgtggcagaa ctgccgggtg tttctcttga aaagccacaa ggagcttttt atctttttcc
     3961 taatgtaaaa gaaacgctac gcatgtgtaa atatgaaaat gtcacacaat gggtagaaga
     4021 tcttttagaa gaaacagggg tagcgcttgt gactggcgaa ggctttgggg caccagaaaa
     4081 tgtccgcatg agttatgcta ctgatcgcgc tacgttagaa gaagcggtac gcagaatcaa
     4141 gcaatttatt gagagtaaaa gtcaaaatta atcctaaaga aattcaagga atataaaagg
     4201 agagtcatac gtggaacaaa ttcaaatcat cgattcgaaa aaccatgtgg gagaaacagt
     4261 aacaatcggc gcttgggtcg caaataagcg atcaagcgga aaaatcgcat ttttacaatt
     4321 aagagacgga acagcctttt tccaaggtgt agttgtaaaa agcgaagtgt cggaagaagt
     4381 tttccaaaca gctaaaaatc tacaccaaga aacatccgtg ttagtaactg gagaaatccg
     4441 tgaagacagt cgttcaaaat ttggttatga aatcggaatt acaaatgtgg aagtggtaag
     4501 cgagagccac gattatccga tcacaccaaa agaacacgga acagacttct tgatggatca
     4561 tcgtcattta tggctgcgtt cttcaagaca acatgcgatt atgcagatca gaaatgaaat
     4621 cattcgtgca acgtatgaat tctttaatca acatcatttt gtcaaaattg atccgccgat
     4681 cttgactggt tctgcaccag aaggcactac ggatctattc gaaacaaatt actttgatca
     4741 aaaagcttat ttatctcaaa gtggccaatt atacatggaa gctgctgcta tggctttcgg
     4801 aaaagtcttt tcattcggtc cgactttccg tgctgaaaaa tcaaaaacac gacgtcattt
     4861 gatcgaattc tggatgatcg aaccagaaat ggctttcact catcaagaag aaagtttaga
     4921 agtacaggaa caatatgtag cattcttagt acaaaatgtt ttagatcatt gcgaatatgc
     4981 attagatgta ctaggacgag acaaagaagt cttaaaaact tatacaaaat tgccattccc
     5041 tcgtattact tatgatgatg cgatcgaatt actgaagaaa aatggatttg atgacatcga
     5101 atggggcgaa gactttggtt cacctcatga aacgttcatt gcgaattcat tcgaacagcc
     5161 tgtctttatc ctaaactatc caaaagccat caagccattc tacatgaaac cacatccaac
     5221 aagagaagac gtggtgatct gtgcagatat gatcgcacca gaaggttacg gtgaaattat
     5281 tggcggaagc gaacgggcaa cagactatga tttcttgcta gaacaaatcc gtaatcacgg
     5341 tttggatgaa aaagaatatt catggtatct tgatctacgt aaatacggtt ctgttccgca
     5401 ctctggtttt ggtctaggtc ttgaacgaac agtcacttgg attgctggta tcgaccatgt
     5461 acgtgaagct attccattcc cacgtttgtt gaaccgaatt tatccataag aggtgatgaa
     5521 aaagctgttc tgcatcaagt aattagaaca gccaagcaaa aacagctgtt catgtttttg
     5581 aattggcggg acttattacc gcagatgtaa gcttttgaat accgtttagt ggagaggt
//
LOCUS       DACENJ010000159         5517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.165, whole genome shotgun sequence.
ACCESSION   DACENJ010000159 DACENJ010000000
VERSION     DACENJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.165"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(24..977)
                     /gene="menA"
                     /locus_tag="IW748_002116"
     CDS             complement(24..977)
                     /gene="menA"
                     /locus_tag="IW748_002116"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1105022.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(990..2093)
                     /locus_tag="IW748_002117"
     CDS             complement(990..2093)
                     /locus_tag="IW748_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1105023.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2183..3118)
                     /locus_tag="IW748_002118"
     CDS             complement(2183..3118)
                     /locus_tag="IW748_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1105024.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3420..5354
                     /locus_tag="IW748_002119"
     CDS             3420..5354
                     /locus_tag="IW748_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1105025.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT         1604 a         1085 c         1098 g         1730 t
ORIGIN      
        1 tttttcacaa cctcttcatg ctgttaaatg gcttgcataa tgattgttag taatagcccc
       61 aatacataac tggcattgaa taagaccata tttttgatgg aatagctgaa actgataggc
      121 tggggtaacg tttgcttgaa ttgatccaaa tgcttcttca caattggcaa ggtcaagaag
      181 gtgaccaaga tcggccattc atagataccg ctcaacaacc ccattaaaat cacgaaatag
      241 caagcataca tcaatagctg gaataagact gttcccgcag gacgtccgat ataaaaaact
      301 agtgtatagc gatgattttc aatatcacgg tcaagatccc gcaaattatt agccaacatg
      361 atattggcaa tcgtaaacac caagggtaag gaagctaata cgattgctgc tatagcccaa
      421 agatttccta ccaaatcaaa ttgtccagat gaaaagttga ctgtcagatc atagacatct
      481 gaaccgaatg tattgacata aacgaccatg gcaaaaattc caaggcccat agtcacccca
      541 ctgaaaattt cccctaaagg cattcgagat aaaggaatcg gtccgaatgt atagaatact
      601 ccaataaaac aacagataaa tcccatcaaa agtaaaagcc aacccgtatt gatcgttaga
      661 ataacgccga taacaaaaac aaatgcaatc atagctaaaa tcatgtttcg aatcaataca
      721 ggagatagat ttctgcggcc aatgacattt tcttcatact tatatacttc agaatgagcc
      781 ttttcataat ccatgtaatt attgatagcg gtagtcgcta aatcaaaaac tagcattcca
      841 ataaaaaata aaaaagtgtt tagccaatgt atctcatgaa aaaaagcgat agagaataat
      901 acccctaccg caaaaggaaa tacactagcc acctttgttc gtatttcaac tacttctaaa
      961 aagattttta gtgtcatggt tcgtactcct tattttagtt cagaacggtc acccatcgtg
     1021 taatgggagt cctccgataa tttgaaggtg tctttgattg gatcggtcac atatacttta
     1081 tcgtcttttg tcacgaagat cgcttccgtc ccgttattca attcgctttc gacgtattcc
     1141 agtccttttt tcacacccat tgaaaagact gctgtagaca acccgtcacc atcaattgat
     1201 ttatctgtta tgatcgtgac acttgcgata tcattgtcga acggataacc cgtttttgga
     1261 tcaaacaaat gatggtattt tttgccatct acttctaaat aacgctcgta aattccagag
     1321 gtaactaatg ttttgttccg ttctttaata gaacctaaaa cagatccgcg agccatgttc
     1381 ggatcttgga tgccgactgt ccagtcttgg ttttcgcctc ttgggctgtg gcctaagaca
     1441 tagacattac cgcccaagtc aacgatggca gtggtcactc cttgtttttt caagactttc
     1501 actacttcgt ccgtaatgta acctttggcg attgcaccta aatcgatgat cattcctttt
     1561 tcttttagat aaacagtttt ttctttgtca tttagttcga tcttatgata atccactaat
     1621 ttcaatgcct gatcgatttc ttcttgggaa ggttttctgg cgtcatcaaa accgattcgc
     1681 cataattgtg tgattgcacc aatcgccata tcgaatccgc cttgagaatc ttggctatat
     1741 tcgtaggccc gcttgactaa cgtgtagacg tcatcagaaa cttttactgg tttgatccca
     1801 gcttgctcat tcacttcatc gatctcagaa cctttttgat tgatcgtgat tttatcacca
     1861 agttctttta ctcgatcgaa ggcaggtttc aatgctgatt ctttgccttc atcatacaca
     1921 cggatacgaa cgtaagtccc taacaagaac tgttcatcag aatatggatc tttcaatatc
     1981 ttggtagctg ctgaactttc agtggttgtt ttatttgctg agttgctgca gccagttagc
     2041 accataaaag agatgcccag tgtgattaag gaaaatagta gttttttccg catctttttc
     2101 ctctcttcat tttgaaaggt gagacgactg cctaatctgg ctatcgtccc accctccaac
     2161 ttttattagt atgattatgt gattattttt ctgttacgat attatctact tcgattgttt
     2221 gagtgtcgcc tttttcagct gcattcacta attgttgtgc gtacatgatg aatgaatgtg
     2281 agctgtgtgt agcacctgta acaacttcta cattagcagg tgagccatct tcttcgccca
     2341 ttgctttgac gaattcgtcg ttcagagtag ggatgtaagt ttttggttct gtaccgcttt
     2401 ttgctttcat attttcatta tattctgtat catcttgttt tgatttaccg tctttgtcga
     2461 tataatcaaa gtttgattca gttactttgc cgccagcaac tgtcatttcg aattgcacac
     2521 ggtagccgtt agagtagttc ttttcttcaa gcttgtaagt gccgtctttc aaatcagcac
     2581 cattgtcgat ttcgattgta tctgtgttac cagcttgagc agcttggatc aattgttgtg
     2641 cgtagttttg gaagctttca gatgagtgag tagctccagt tacgacttcc acaccgcttg
     2701 cgctttgtgc ttttaagaat tggtcattca actcaggaat gtatttttct gggtttgtgc
     2761 cagattttgc ttccatgttt ttgttgtatt ctgcatcttt tgtttttgat tcgccgtttt
     2821 cattgacgtt atcatatttt gattcagtga ttttgccgtc ttttacaacc atttcaaaga
     2881 ctgcacggta accatttgaa taattttttt cttctaattt atatgtgccg tcttgcaaat
     2941 ctccgccagc tacaactttt gcagatgatt ctgttgtgct ttttgcagtc gaagattgtg
     3001 ctgtagatga ttcagaagaa gagtttgagc ttccgttgtc atctgcgccg caagcgccta
     3061 acactagagc tgaaaatgct aatacggcaa atccagttac aaaacgtttt ttcttcattt
     3121 taatacccca ccattccatt tctagtccac aatttttctt tgtgattcat tatacattct
     3181 aatcttttta gcctataagt caactatttt tgataaataa cccatgtaat atttcaaacg
     3241 ttttctttca gcaaatttcg tgaaaaaacg ctttatttta aaatttgaaa aaaatccaaa
     3301 aggagttaga caaaagaaca gaacatctat ataatatatg gagattgtgt aataagtatc
     3361 aaactaaaag aattatcata tattcattat atagatagtc tataaggagc ggatgatcaa
     3421 tggcaaagca aaatattgtc gttgtaggag caggatatgc cggtgtttca gctacaaaat
     3481 acttggcaaa gaaactgaaa aaagaagatg tagcaattac tctgatcgat cgtcattcgt
     3541 accatactat gatgaccgag ttgcatgaag tagctggcgg acgtgtagaa ccagaagcaa
     3601 tccaatacga tctgcagcat ttgtttgctc gtcaaaagaa tgtacaactt gtcacagaca
     3661 ctgtaatcgg tatcgataaa gaacgaaaaa tcgtcaagac caaattaggt tcctatcctt
     3721 ttgatcagct gatccttgga atgggcggag agccgaatga ctttggtaca cctggtgtca
     3781 aagcacacgg attcaccctt tggtcatttg aagattctgt aaggattcgg gaacatattg
     3841 agaagacagt agcaaaagct gcattggaac cagatgatga actgcgtaaa gccatgctga
     3901 ctttcgttgt ctgtggttct ggatttaccg gtattgaaat gattggggaa ctgatcgact
     3961 ggaaaaatcg cctagccaaa gattataaat tagatccaaa cgaaatcaca ctgatggtcg
     4021 tagaggcaat gccgacgatc ttgaatatgt tggatcgaaa tgatgctgca aaagctgaac
     4081 gctatttgcg taagcacaac gtggaattat tattgaatgc accaatcgta gaagtggctg
     4141 ctgatcatat caaattgaaa gacggacgca acattccgac tcacacactg atttggacag
     4201 ctggtgtcaa aggaacttct gacgctgctg actttggatt agaatcagca cgcggacagc
     4261 ggttgatcgc caatgaatat atgcaggcaa aaggctacga agataaaaac atctatatca
     4321 ttggtgattt agtcttttat gaagaattcc caggaacacc gacaccgcag atcgttcaag
     4381 ctgctgaaca aacaggacac acagccgcag cgaatatcgt ggcttcgatc aaaggcggag
     4441 aaaagcataa atttaaagga aactatcaag gatttatggt ttctattggt gccaaatggg
     4501 gggtagctaa cctatttgat aagatccatt taagcggatt tttagctatc gtaatgaaac
     4561 atatcgtgaa tttgaaatac ttctttgata ttcgttctgg ttattatatg ttccaatatt
     4621 taatgcatga gtttttccat atcaaagatg atcgcaacgt gactcgagga cattcttctc
     4681 gttacggcaa tgtcttatgg agtgtgccac ttcgtatttt ctatggttta gtttggttcg
     4741 ttgaagcttc caaaaaaatc attgggaatg gtgaagtgtt caaaccaagt acttggtttg
     4801 gtgaaggatc atggttcact ggaaacagtg tgttcccttg gacatgggaa tacaatgtaa
     4861 cagatgttgc cagtggtgct tctgctgctg gtgatgcaac cacagctgcc agcggagcag
     4921 gggcaagtgc cggagaagca gcgactcaag ctgcacattt tggtttgagt tatgcatacg
     4981 gtgaagaacc aatggctgtg attgagaagt caccagactg gttcacaaag atcatgcaag
     5041 tgatcatgcc aaacaatgat gtagcaatgt tcttccaaaa attcatggta ttctttgaaa
     5101 ttgccttggc attagcattg attgctggat tattcacatg gctatgcagt gcgacaacga
     5161 ttgtattggt gatcacgttc tgcttatcag gcatgtttta ctgggtaaac atttggttca
     5221 tttttgttgc tttcgcattg atgaacggtt ccggacgtgc gattgggttg gatcgctggg
     5281 taattccatg gctacaacgt acactaggta attggtggta cggtagacca agttcccagt
     5341 acggcaagaa ataaatagat aaagaacgtt atttgaatgc aagatttcaa tagaaagggg
     5401 ctgagacaaa ccgtcacagc ctccttttta tgtaaaatta aaggaataag atgcccgaga
     5461 taagcctcca tgagcaagac acaggtgtca caacctctgt tacaacctct tgttata
//
LOCUS       DACENJ010000160         5511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.166, whole genome shotgun sequence.
ACCESSION   DACENJ010000160 DACENJ010000000
VERSION     DACENJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.166"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(67..537)
                     /locus_tag="IW748_002120"
     CDS             complement(67..537)
                     /locus_tag="IW748_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1105026.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            867..2507
                     /locus_tag="IW748_002121"
     CDS             867..2507
                     /locus_tag="IW748_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1105027.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2661..3983
                     /locus_tag="IW748_002122"
     CDS             2661..3983
                     /locus_tag="IW748_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1105028.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4051..4485)
                     /locus_tag="IW748_002123"
     CDS             complement(4051..4485)
                     /locus_tag="IW748_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1105029.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4550..5386)
                     /locus_tag="IW748_002124"
     CDS             complement(4550..5386)
                     /locus_tag="IW748_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1105030.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1645 a         1099 c         1003 g         1764 t
ORIGIN      
        1 tttttcacga cctctgattc acggtgtttc tttttcatca acatttttac gaaatactgt
       61 ggaagactat tgaaataagt gtggattgtt tctttgtgtt tcaatcgttt cttgccattc
      121 ttcatttaat tttggattac cgttttcttc cttgaactca taaggtccta attcaaaaat
      181 cccagacagt atttgattca cgatttcata tagttcagtg tgatcagaca catgattctt
      241 gaagatcatc tcattatcac cacgtttgaa cctcacattc gggctctcat gatgaacctc
      301 tatgcatttt tctccttttt ttaactgacg gacttctgaa aacatctcat aaatatcttc
      361 tccagtaaaa ccttcagcaa gcgccagact gtccacaaga ccgcctactg tcagttcaga
      421 gattattgct tgattggaat ctaattttaa tgtatagctt gtcgcttcta ctacgccaca
      481 tgcattgcaa ccgccttctg atagttcgct ttttctaatc aattgaactt ctttcatttt
      541 ctcttcctcc tcttaatttt ctacttatgt atttttatca cctgacatag gtgactccta
      601 tcaacgctat catgtgcttt tttcctcaga aatcctattt atccattcca tctatccctt
      661 attcttctac ttttctttct ctagcatata agagatacgc atagtattca agcaaggatt
      721 gtcgatataa aaagaacaaa tactgctatt ttgatttctt ttggctatat tacatataaa
      781 taaaatttat catacctgct ctttttgact atatcatcat ctgacttcta tacttcgttt
      841 gtattaaaac ttaggaggaa tcattcatgc ttacagggtt aaacttatta aataatcctt
      901 ttctaaataa aggaacagct tttacaaaag aagaacgggc aaaatatgga atcactggta
      961 tgcttccaag tactgtccaa acactggaac aacaatctgt tcaagcttat ggtcaatact
     1021 taagcaaaca aacagatttg gaaaaacgta tcttcttaat gaatctattc aatacgaatc
     1081 ggacattgtt ttataaattg atgggcgaac atttagttga attcatgcct gtcgtttatg
     1141 atcctgtcgt tgcggattca atcgaacaat acaacgaaat tttcttgaaa ccgcaagatg
     1201 ctgcatttct ttcaatcgac gaaccagaaa acatcaaagc aagtttgaag aacgcggcag
     1261 atggacgaga cattcgtttg attgttgtga ctgatgcaga aggaatctta ggtatgggcg
     1321 actggggtgt gaacggtgtt gacatcgcta tcggcaaatt gatggtctat actgctgctg
     1381 ctggtattaa tccagcacaa gttttgcctg tatcgatcga cgctggtact aataatgaag
     1441 aattattgaa taatccctta tacttaggca atcgtcatgc tcgtgtagaa ggcgaaactt
     1501 actatgaatt catcgatcaa ttcgttcaat cagctaccga attattccca gaattgttat
     1561 tgcattggga agattttgga cgaggaaatg ctgcagcgat cttagaaaaa tacgaagatc
     1621 aaatcactac tttcaacgac gatatccaag gtacaggaat tgttgtccta gcaggtgtat
     1681 taggcggatt gaatatatcc ggtgaacaat tgaaagatca aataattttg acatttggtg
     1741 ctggtactgc tggtgtgggt atcgcaaaca tccttttaga tgagatgatc cgtcaaggtg
     1801 tacctgaaga agaagcacgc caacatttct atcaagtaga taaacaaggt ttattgtttg
     1861 aagatacaga aggattgact cctggtcaaa tcccattcgc tcgcaaacgc tcagatttca
     1921 ctaatgctga tgaattgaca aacttagaag cagttgtaaa agcgatccat ccgacaatca
     1981 tgattggtac ttctacccaa cctggagcat tcactgaaga aatcgtgaaa gaaatggctg
     2041 ctcatacacc acgtccgatc atcatgcctt tgtctaatcc aacaaaattg gcagaagcaa
     2101 aagcaaaaga tttaatagaa tggacggacg gaaaagcatt agttggaaca ggtatccctg
     2161 ctgctgatgt tgaatataat ggtgtaactt accaaatcgg tcaagcaaat aatgctttga
     2221 tgtacccagg tttaggactt ggtttgattg cttcaacagc tacacgagta aatgctgaaa
     2281 tcatttctca agctagtcga gcattaggcg gaatcgttga tgtaacaaaa ccaggagcag
     2341 ctatcttgcc tccagtagca aaaatcactg aattttctca aacgattgct gaaactgtcg
     2401 caaaatcagt tgtcgcacaa aacttgaatc gtgaagaaat cacggatatc aaagaagcag
     2461 ttgaatctgc taaatgggtt cctgaatata aatctttaga agattaagat cgttatagta
     2521 aaagaacata cagaaacttt gtatgacgtc taaaaagaaa aaagcgactg cggcaaaaca
     2581 gcggccagcc aactcctgct cttttgtcgc ttttctttgg cttttttcat atgatacaga
     2641 ataagaaagg atctgtaaaa atggcacaaa aaacaacaac tgcaactgca gttgaggaaa
     2701 agaaaaaaga aggtttttgg tccacaaaaa tcaccggcgt aagtttgcca ctttatttga
     2761 tcatggtcct tgtcctgatc attgcaatgg ctttagggaa attaccgacg aatatgattg
     2821 gaccaatcgc agttttggtt attttcggta atttatttca ttttatcggg acaaaaatcc
     2881 ctatcgtcaa aagttatttg ggtggtggtt ccgtttttgc gatcttcgct tctgctgcga
     2941 ttgctacatt tgggatctta cctgaatatg tagtgaaatc cacagaaaac tttgtcagca
     3001 atatgggctt ttgggacttt tatattgctg ccttgattac tggtagcatc cttggtatga
     3061 accgtaatct attgatgaaa gcttctgttc gttttattcc cgttgcactt atttcaatgg
     3121 tcgtctcctt ttttgccgta ggactcgtgg gcatgcttat cggaaatggt tttgcaaatt
     3181 ccgttttata catctcatta cctgcgatgg ctggtggtgt tggagccgga gccgttcctt
     3241 tatcaacgat ttatgcaaat gttttaggta cggatgcttc ttctatcatt tcccgcctga
     3301 ttcctgcgac agcaatgaca aatgtcttag ctatcatcgg tgcagcatta gttgctcgtg
     3361 ctggacaatc aatgccaaaa ttcaacggca atggtaaatt aatggaaaaa ggcgatcttg
     3421 gcgatggaaa acctcgaact gtgaaacctt ccattccaca gttaggtgtt ggtttgatgg
     3481 tatcattgac tttcttcatc ttaggacaaa ttggtaacta ttttgttcct aaagtccatg
     3541 cttacgcatt tatgattatt atcgttgtca tctgtaaaat cgccaaccta ttaccagagt
     3601 attatgaaga tgcagctatc atgttcaata acttgattgt caaaaacttg acagcagctg
     3661 ttcttgccgg tatcggtatt gcgttgctga atctgaatgt attagcttct gctttgacat
     3721 ggcaatttgt tgtcttatgt ctgacaagtg ttatcgcgat ttcagttgtt tcaggatttg
     3781 tcggtcgttt gtttggttta tatccaattg aatcaactat tactgctggc ttatgcaaca
     3841 actcgatggg cggaacagga aatgtagccg tcttatctgc agctaatcgg atggaactta
     3901 ttgcttttgc tcaaatgggc aatcgacttg gtggtgccat cgttttgatc atctctggtt
     3961 tcttgatgca attactttct tagtcaaaaa aacaaaaata cctgatcatc aagtatcggc
     4021 ttggtgatcg ggtatttttt tgataaagat ttacaggatt ttcaaaaatc ttttttgcat
     4081 tttatcggaa tgataaccga tgtgttcata aaattggtgt gcttctactc tgtattctgc
     4141 agaattaagc cttattgtcg ttattcctcg ttttttcgct tcttcttcta cgccagtcat
     4201 caaagccttt ccgatgcctc ttttttcagc atatttagac acagcaagtg ccataatatt
     4261 gaacattgga tcgctaaaaa tcgattcata ggactccgct tgtacatagc cagctgcttt
     4321 tttagaagta ggatcttcat aaaccaagaa aaaatttttc tctggctcct tcagcagttt
     4381 cttcatttgt ctcaccgtaa cggttaatgg tatatcatac cctaattgat ccgcattgat
     4441 ttcacgtagt gcttctgcat cttctaaacg catttcccgt aacatccaaa tcaccagcca
     4501 atttctgatt ttttaaaatg aattttttta tcatagtata ctcttttttt catttagaac
     4561 aatatgtttc agtgattttg acttgcgtta gtctagattc tcctaatgtt tgtaagatga
     4621 caaaggcatt gtaacaattg accaaaagaa ccacgaggac aattacttga aaaataaagg
     4681 tcactttttt tggagacaaa accctgctaa atcttgctcc ccacaaacct ccaataacag
     4741 cagcggggat aacataaaac aacatagata aatcaaagcg cataaagcta cttgttaaag
     4801 caatcgttac caattttgcc agttgagaaa agaaaatcgt agatagagaa tatagtgttg
     4861 cttcttttat tggcaatgca aacatcagca tcaacaaaga aacattgact ggtccaccac
     4921 caatacctaa aaaactagct aaaaagccca gaaccccccc acatataagg taccaagtcc
     4981 atgacgtcaa atgaaatttt ggctgatgat gcttggtgta gaagaacgca aaaaccaagg
     5041 tcaataccgt caaaaaaatt tggatcagct gcacatgctt ctcgttttca aataactgta
     5101 aaaaaacttc gaatacaatg ttgccgacta ctcctccaac aacagctcca ctagataccc
     5161 aaccgacaat ctgccaattc agtgatttcc cgcttgatac ttgtcgcaca gtcgaaacaa
     5221 tggacatcgt gaataccgca actgttgagt aaaaagaaat ccctgcaaca gaatgggcgc
     5281 cgataagatc aagaatcggt ttgatcaaca cgcctccacc catcccggaa atagcgccaa
     5341 ctgtgttcgc cagaacgatc accacaaaat aaatcagacc aatcatcttg tatttccttt
     5401 ctcttgttta tatcttttag cgattgttac tcttttcttt tattatacag taaaagaaac
     5461 aaacgaggct tcgaaagctt ttctactttt taaggaaatt tagtgctaaa c
//
LOCUS       DACENJ010000161         5500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.167, whole genome shotgun sequence.
ACCESSION   DACENJ010000161 DACENJ010000000
VERSION     DACENJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.167"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            74..2134
                     /locus_tag="IW748_002125"
     CDS             74..2134
                     /locus_tag="IW748_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105031.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2217..2786
                     /locus_tag="IW748_002126"
     CDS             2217..2786
                     /locus_tag="IW748_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1105032.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2961..3767)
                     /locus_tag="IW748_002127"
     CDS             complement(2961..3767)
                     /locus_tag="IW748_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105033.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3905..5419
                     /locus_tag="IW748_002128"
     CDS             3905..5419
                     /locus_tag="IW748_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1105034.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1954 a          954 c         1118 g         1474 t
ORIGIN      
        1 atttatataa tgaaatcatc ttatgaaaat tactacttca tgtttagaca agagtaggga
       61 ggactaagaa taaatgtgtt caaaaagacg ttcaaaacaa ctaaataaac acgcttgtcg
      121 tttagataag gaacggtcgt gttctgaaaa acactacgtt aagtttatga tgcgcacgat
      181 gctgatcagt ttatctgtag gtgtctatat gcatgtaccc gttcaggcaa cgacacaaac
      241 catcaaggtc gtacgtacct ctgtttctga gaccactgat tcccctatca ctattactca
      301 tactttcact gatgcaaatg gaaaacagct acttcctgca ttgacttcct ataataaagt
      361 cagttatcct ccaacgataa accactactg gttaattcct aacaaaacat tatggacatc
      421 ggaaaccgga agcagtgtgc cgctttctat cttttccgat atgactgatc ctacaaaagg
      481 tgatcagtta aaacagatca ctgatatttt gaatcatgct ttgacagata taaaggacgg
      541 tggaagcgca caagtttctt atgtatatga aaaagattat tcacagtttg aaagaaagaa
      601 aatcgatgtt ctggcaggga caaaagtttc gatatctgat ctggtagaaa ggctgaagaa
      661 cgcagatgga acacaaggag atatgtccaa agtaatttca aacaatggga atatcgtgga
      721 tacgactaat acaggaacat ctactatttc cttgtcctat tttgatccaa ttgctttaaa
      781 aacaatgact gcatctgctg tgatccatgt tcgtgctgat caaacagcta tcaaaggaaa
      841 agatcttcac ttaagaacag gagagagtta cgcccctaaa gatcttgttg aatatgtaac
      901 aaaaagcgat ggaacttcag gaaataaaga agaaatcaaa atgaacggga cagtagatac
      961 tacaactccg ggagaatatc ccgttgtatt aacttatata gactcgaaaa catcaaatgt
     1021 ggcagaaaca acagctgtag tttatgtaga agcatctgct gcttcatcga ctgtcatcac
     1081 tcattttata gatagtcaaa caaataaaga acttcaagca gaaacacata tgagtagtgt
     1141 aaatcgtatt cctgatttgc gcctacctgc tgaattttct gatcgagtgc caaatataga
     1201 tttatcgact tatgaacaaa atgggaaagt cacttctatt tctgattgga tgcaagcaaa
     1261 cggattagac agaaaaagtc actggtcgga gatccttgaa gtacttcact cagaactgat
     1321 gaataatagg aagcaatcag gaggggaaat tacctttact tatgtttata taccagacaa
     1381 tcgttcatta gaaggtcatg atcttattgt tcatccaaac gaaaaaataa acaaagaaga
     1441 gttgatccaa tcagcaacaa attccttagg taaacccgta ccgttaaagg atgtatcggt
     1501 tacaatcaat gaagtaccat tggaggatgg ttatttgacc aaacaaacag gtgattttac
     1561 gattatgtat agttatcatg acccgtattc actccaagaa ctccatgctc agtcactatt
     1621 acatgtttta ccattaaaca aacagataga tcaaaaagaa caaaaaagac aagaagaaag
     1681 cttgtcagaa aaaaaacaac aaatggctca tgcagtttct ccaacaggga aagtaaaaca
     1741 aacaagaagc ctagagttag caagaatatc tagaaaaatc tcagaatcta ataaaacgat
     1801 aaggcaagaa gctcatttgg ccccacaagt ttctggattg gaaaatgaaa ttttagagcc
     1861 taaaataaaa caacaatcag aaaaagaatt tgttgctaaa cagtcggaag atcagaagga
     1921 atcagatgac cagaaggaaa cgaagaaaaa acgcttgtca actattgatt ccaccgatga
     1981 gcatctgcag ccgcaagtac cacttcctag tccgggaaca cccgcacctg gtggaggtat
     2041 tccttcttct gctttaggag atttcatgcg aggtatggca ggaacatggg tatacgctaa
     2101 tcgaaatgaa tttgacgcag atgactggtt ctgatttata tctctatctg ttatagtcaa
     2161 ataagtagct gagtcatatg ttatttggca taatggagac gaggagggat agaaaaatgt
     2221 tagataaaac gttaccttac gcagaaatct ggatgacaag accacttcaa caaaatgttc
     2281 caatcacgcc tttagcggaa ggttttgaat ttcgatttta tcaaaaagaa gatgaatctg
     2341 cttgggcgga gattgagact tccgttgggg aatttcaaac gacagatgaa gcacttgagt
     2401 attttcaaca aaactttgct ccttatcctg aagaacttga aagaaggatg ttttttgtag
     2461 agacaaaaaa aggagaaaaa gtagcaacct gtaccgcttg gcgaaaaaaa tcaagagagc
     2521 aagtgtatcc ggtttttcat tggttagcag tgaaaccaga ttaccaagga aaagggctcg
     2581 caaaagcatt gactgccaga gtcttgcaag aatttcctac cctccatacg gacggaccaa
     2641 tctatcttca cacacagaca tggagccatc aggcgattgc tctttataaa aaaatgggtt
     2701 ttacttttat tgctgaaaat ctagacggca cacctaatcc agattatgaa aaagttatgc
     2761 aggttttaag taaattagat aaataacgaa atggtgaaat aagtcctcag tattgggata
     2821 aaaaacgaaa tagtcattcc tcatatctta cttttttgag ttaatactat cggataacta
     2881 atggcatgcc atacattcaa aaaagaaggg gcagtgacaa aagtattgtc actgcccctt
     2941 ctcttgtttt ttagcatgtt ttatcgtttg atttttttag gaataaaatt cttgatgaac
     3001 gtaaattttt ggtaatcgtt tcgactatag aggtaaccaa atacactgaa aacaactgct
     3061 ccaataaaat taacaaataa atccttcata gtatccatga ttcccacgtc taagtaacca
     3121 ttttggatga ttgttttatc tgttttacca tctgcatttt gactttcaat gattgtccga
     3181 gtgatatctg gaacatgata gacagtgttt ccgtctttac ttaactccac actattgatt
     3241 tgctggatga tcgtatcttt ttgcatatcg aattgcagta atttatctgc tgaaaattcc
     3301 acaaactccc acataacacc aactgtcatc gaaaaacaga aggaaacaac tgctagtaat
     3361 aaaggagtca agttcatggt ttttgtattt ttattgagca aataaacaag ggaaaagccg
     3421 acacccccag ctaaaaatcc gctgaatgca tgtagaaaag tatcccacat tgaaaaatag
     3481 ccataaaaat cacttaactc tcctaaaact gttgaactaa aaataaaaga taacaaaatg
     3541 atttctagca tatttggaat ctgtatcttt aatatctttt ccagaaaata agggatttca
     3601 aataaaatta atgtcagtaa taaaagcatt gtatttcccc agtgttggtt ccagacctga
     3661 tccactagca taataacgac gatgatacgt aataaaatat agattccgtt tgtgattcta
     3721 cctgcagttt ttgtactgtt cgtaaaaaag gttttcaaat gattcataaa ataactcctt
     3781 catgtatatc cttaattata ctaagaagcg tgtgtttacc aaagctatat gacttaaaga
     3841 aaaacagagt tgatattgtg aaaaaaatca cttaatatag tataaggaga aaagggggat
     3901 acaaatgaaa aaattagttg ctggtttatt aacagccgtt gcacttcttg ctatgagcgc
     3961 atgcggcgca gatcagaaaa atacagcgca gacaagttct agtacgaagg aatctgccga
     4021 agtatcatca ggcgcttctg aacaagtata tacagatcca aaagaaatga aagaatctta
     4081 tgatgtcgtg attattggag ctggtggtgc agggatggca gcagctatcg aagcaaaaga
     4141 tgctgggttg aatccagtaa ttttggaaaa gatgcctgtt gccggaggaa acacatctaa
     4201 atcatcaagc ggtatgaatg cttcagaaac aaaatttcaa aaagaagaag gaatcaaaga
     4261 ctcgaatgat gccttctatg aagaaacgct aaaaggtggt ggaggaacga atgataaaga
     4321 aatgcttcga tactttgtcg atcactctgc tgatgcggtc gattggctag atcaaaacgg
     4381 tatcaaactg gacaacttaa cgatcactgg agggatgagt gaaaagagga cacatcgtcc
     4441 gtctgatggc tcggctattg gtggatactt agtggagggg ttattgaata acgtccatga
     4501 gcgggaaatc cctatttttg tcaacgcaga tgtgacagac attaagaaaa atggggaaac
     4561 ggtcaatgaa gtgactgtac atgttcaagg agaagaacca aaagaggtca ccggaaaagc
     4621 aatcgtagtt accacaggcg gttttggtgc cagcaaagaa tttattgaaa aatatcgtcc
     4681 cgatttagct gattatatta cgacaaatca agaaggatca actggagatg gtatcaccat
     4741 gattgaaaaa ttaggtggac aaaccgttga tatggataaa atacaaattc atccaactgt
     4801 gcagcaggat gagggggtct tgattggtga ggttgttcgc ggagaaggag caatcttggt
     4861 cgatcaagag gggaaaagat ttgtcaatga aatggataca agggataaag tatctgcagc
     4921 aattacagca ttgcctgaaa aatcagctta tttgattttt gaccaaggcg tgcgtgatcg
     4981 tgccacagct attgaatttt atgaccaaaa aggatatgtc acaactggaa agacaatgga
     5041 agaattagca gaaaaaatcg atttaccaaa agaaacacta gcaaaaacag ttgatggatg
     5101 gaatacggat gttgcggaga aaaaagatgc tcaattcaac cgtgccacag ccatggaaca
     5161 tgacttatcg tctccgaatt attatgcgat caaaatcgca ccaggaatcc attacacgat
     5221 gggcggtgtg aaaatcaata cgaacactga agtattagat aaagaaaacc agccaattag
     5281 ggggttatat gctgctggtg aagtcacagg tggcttacat ggaaacaacc ggatcggtgg
     5341 aaattcagtt gcggagatca ttatttttgg gcgacaagcg ggcatacaag cagctcgatt
     5401 tgcttcggaa aatgaatgaa atagaacgta aataaaaaga ggttgtgaaa tcagcgtttt
     5461 gagctaatac cgagggtcag ctgattgaac actgtttagt
//
LOCUS       DACENJ010000162         5430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.168, whole genome shotgun sequence.
ACCESSION   DACENJ010000162 DACENJ010000000
VERSION     DACENJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.168"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(54..404)
                     /locus_tag="IW748_002129"
     CDS             complement(54..404)
                     /locus_tag="IW748_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105035.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(397..1722)
                     /locus_tag="IW748_002130"
     CDS             complement(397..1722)
                     /locus_tag="IW748_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1105036.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            complement(1891..2475)
                     /locus_tag="IW748_002131"
     CDS             complement(1891..2475)
                     /locus_tag="IW748_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1105037.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(2686..2889)
                     /locus_tag="IW748_002132"
     CDS             complement(2686..2889)
                     /locus_tag="IW748_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105038.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(2899..3321)
                     /locus_tag="IW748_002133"
     CDS             complement(2899..3321)
                     /locus_tag="IW748_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105039.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            3635..4249
                     /locus_tag="IW748_002134"
     CDS             3635..4249
                     /locus_tag="IW748_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1105040.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(4414..5418)
                     /locus_tag="IW748_002135"
     CDS             complement(4414..5418)
                     /locus_tag="IW748_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105041.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
BASE COUNT         1680 a         1021 c          803 g         1926 t
ORIGIN      
        1 ttctgatagc tgatcaaagg ttatatactt tagctcatat tgctggataa tgttcattct
       61 aatcgactcc atttattatc ctggtaaaat tcgatatgac gtatttcgtc gtagtgaatt
      121 ttgtcttttc caacgtagat ccccagctca tcactaccta agatcttgcc aatgatatct
      181 tctggatagt atccttcagc gtttcgttct tctttttgaa tagatactga tctatttttt
      241 atgactgcag cgttcaatac ttccgcaatc tcttcagtcg tcatttgttc ttttgctaaa
      301 ttttgactgg ttcgttgttc attgtcggca tcaatttctt cagtatgatc agataaataa
      361 aaccctgccc actttaaacg tccacgatca ttgtaatcaa tcatttttaa ttcccccatt
      421 tccgcccgca tgaccaccaa caagtgtgct tctagtaatg cttctagcgc cttctaacat
      481 tgaagaagca tgtacaatcg acgtaaaacc atatttttgc cgaatcttgt caataatttt
      541 atctaaattt tcttcgtcaa tttgcttttg tggatcacta aataaatcca gctgaagcga
      601 atcggtgtac acaagcttgc tataggttat tccaacatgc cttacttctt gtccttcata
      661 atacttatca aacaagaaaa gacaataatt ctgcaattcc tttgtattat ctgtgatagg
      721 gatcttcatc tggtgtgaaa accctggtct cgtttctaag atcgacgtgc caatgtgtaa
      781 atggacacaa cctgttttac agttatgctg cctaatgcgt atcgctactt gttccgccat
      841 ttcttttacc accagctcga tttcatttct tctagtataa tctcgtggta acacttgtga
      901 atttccatat gatttttcct ttgtcggttc tggtgggatt gctatatctg ttcgatcgat
      961 cccattagca tggaagtaca gctgtaatcc aatcacacct aatctattct taatcgtata
     1021 aggattccag ttagcaaggt ctctaatgct caaaattccc atttggttga gccttttttt
     1081 cattcgcgaa ccaattcccc aaaagtccgt catttcggga atattccata ctttctcagg
     1141 aacgttttcg tacgtccatt ccgcaataaa tccttcatta tgctttgctt cattgtctaa
     1201 ggctaattta gccaaaaggg gattatcgcc gacgcctacc gtagcaatca aacctaactc
     1261 ttctttaata cgttcttgga tcatttgagc gagcttcttc cttcgctgac ttcgtgtttc
     1321 ttcagtcgta aaaagattca gtgattttgt cacttttaaa atcgattcat cgatcgagta
     1381 aatcagtaga tcttcatcag ccacatatct tctgaaaata ttatttacct gcatatttcg
     1441 cttgatatat aggttcatac gtggtggaac aacgtgtagt gttttaggaa atggttgtgg
     1501 taagtcacgt ggtctactca catttgtaat accataccgc ttttttgctt caggagaaga
     1561 agctaatatc aatcctgaac cagtattatc agatcgactc ataacaacaa gttctgttgt
     1621 taatggatct aaatttcttt ctatacattc gacacttgcg taaaagcttt tcatatcgat
     1681 taaaaagtaa tcatttactg gctcttttga ataatccatc ataaataaca ctccattctt
     1741 ccacattata caaacatatg ttcgatgttg taaagaagaa atagctaaat atttgagaat
     1801 aagttatcag tagacactta caaaaaattc tcatactatt gtcacttttc tagataaatg
     1861 gcaaaaattt tcagcattaa atgacaaaaa ctaatgagaa tttgtctttc catttttctc
     1921 ctccattttt gccaaataac gataaatggt ggttcttgag acgttccacc gttccgcaat
     1981 agtcttaata ggtgtaccgc tatccactaa agtcttcagt aattccaaat cttgttcaga
     2041 caatttttca ggacgaccac caaaccgtcc tcttgcacga gcagctgctc gtcctgctgc
     2101 ggatcgttcc aggatcaaat tacgctcaaa ttctgcaaat gccgcaaaca aatgaaacat
     2161 gagctgtcct gtagaacttg atttgtccat cgtaatattt tcttgaatac tatgaaaact
     2221 cacaccacgc tcattcagtc gattgacgat tgaaatcaaa tcttccatat ttctacctaa
     2281 acgatctaag cgccaaacga caagtgtatc gccagagcga acaaaatcaa tggccatttc
     2341 taatcctggt cgattacttt ttgccccact catatgatca gtaaaaattt tttcacaacc
     2401 aaatttattt aggctgtctt cttgtaaatc caaattttgt aaaccagtag atacccgggc
     2461 gtatcctatt ttcaaaaaaa tcactccttc attaatttta ttgtaacata actttatcca
     2521 tatagtttaa ctgaacatag atattgttat gggtttatga acatgaaatg aattgttttt
     2581 ttgaaaaata aagcaaaaaa agaaagtgtc cagaaatggg tgattttatg aacactttca
     2641 attagaaaaa aagaatgtcg actagacact ctttttttaa aatcattatt tttctttttg
     2701 atatgttttt tggtctcttt tttttctttt ttcttcagaa tcttctagat tagaaattga
     2761 tttttgaatt ttttgtgtct cctgtaaagt tcgctgaact tgtaaagctg attcttcttt
     2821 cggtaaatta attttactta gaatatcgct atcttgataa aaatttggtg tgttcacaaa
     2881 ttctggcata aaaaatccct actttctagt taaggttcct gaataagtcg caatccaatt
     2941 gtttccagct gatttataac tttgtaaata caattttcca tagtaccaat aaccatttct
     3001 tttttctttc caattccgtg attgaggagg tctagaaggc caaccaactt tatcaaaaat
     3061 catcgttcct gtcactcgta tcgttttaga agctgctcga gcaagtaatg gagaaactat
     3121 taaataattt tcagtaacag ttaagtcgtt agttgggtta ccaatggcta ttccgttcct
     3181 atctaaagga gttaattctt ggttattatc tactacagta gttggttcat ttgtactatc
     3241 agcaaatgta ggaattgtag ttccagttac actaattcca atagttaaaa tacctaaaga
     3301 taaaataaac ttatttttca ttgtctatca cctcgaaatc aaaaataaga aaacgttttc
     3361 ttaataaatc ttaacagata aatcaagtta tatactagaa aattcataaa atatattttt
     3421 aatgtcataa tcgtttaaat tttcttgatt gacatttatt tacaaaaaat attgccattt
     3481 aaaaagaaaa gtgacacata ctatttcctc catcattagt ttttgtctat ataaggttta
     3541 ccctaaatag acactggtat aacattgaaa aatatggctt cctcgtgtat gattagggtg
     3601 tattttaaat agttgcagaa agtaggtaaa agttttgaga gttgcataca tacgtgtctc
     3661 atcgatcgat caaaatgaac aacgccagat tgaagaaatg aaaaaattcg gtgcagagag
     3721 aattttcatc gagaaacaat ctggcgcaac gattactcat cgacctgttt tccaagaagc
     3781 attagattac gtccgagatc aagatatttt tattgtggaa gctattgatc gacttggaag
     3841 aaattatgac gagatcatcg cttcagtcaa ttacttgaag aagaaaaatg tgaagctgat
     3901 cattactagt ttaccgatca tggctgaagc aattggcaat cctctcctgg ataaattcat
     3961 taaggatctg atcatccaaa ttttggccat gatcgctgaa caagaacgga ctgaatcgaa
     4021 acgtcgccag gcacaaggaa ttaagatcgc aaaagcaaac ggcgtctaca aagggcggcc
     4081 caaattatac agtgcagacg caaaagatcc ccaacgtcgc cttgtttaca aaagcatcgt
     4141 tgaagatctt aaaaatggtg ttgcgattgc taagattgct aaagactaca atgttacgag
     4201 acaaaccgtg tatagaataa aaaaagacag catggtcaat gacaaatagt tgatcatgct
     4261 gcttttatta tcttctcatc tttaatttcc tacatataat tactatctat aaaattaagt
     4321 cttactcaga acagataaag cataaaattc caacaaacca ttttttactt aactaaataa
     4381 agagtctgtt aatataatct tcacttattt acgttaatcc cctactgtat ttgttggttc
     4441 cttatctata ttttctacaa aatccttatt atcttgtgat ttagcttcgt ataattcttt
     4501 gtatgtcgac acattggtta tactaattcc tatagctagt tcttgagact ctacttttct
     4561 ctctagctcc tttgacaaag tgatttgaga tttatttggt tccgttggag tcaattctat
     4621 agattcttca taaaaatctc tatacataaa gctatcatta attttataaa aaaaattaaa
     4681 tgtttttagc actgccatat aacattcaaa acattcttct atagttggtt tacttccttg
     4741 aggagaatga accaatttat tacgtttttc tatgaaattt ttaatttcct ttcttatttc
     4801 tttcggaaac tcaatattaa agtacccttt aaacatgctc cctaataatt tgtctacagg
     4861 gggagattgt aaattctcaa atattagagt taaatcaggt ttctcttgtt gataaaattc
     4921 ctttacccct atttcacagg ctgtaattat cgaaataaac ctatctctag tatttgatat
     4981 atttttactt cttttaagga gtataagaga aaaggggata ctttcgtgtc catgcatgta
     5041 attaactaat tgttgagctt cagaagttat atctctaatt atatagttaa cggaattagg
     5101 aagtgtcttt aactctaatg aaatttctat ccaattacta tcttctaatc tgtatatgct
     5161 cgaagtataa tttgattcgt cagcaagcat cgctacatcc caaacactga tcataaaatc
     5221 ttttaaacgt tcaatcattt cagcaatatt gctaaaatca gctaattttt ctttagaata
     5281 aaaaaaaaca tttatatcag aatcaataaa ttctattttt tttattacta tatcgtcatc
     5341 attatcttga ccatttatgt ttattgttcc ctgagtgggg agtacttctt tcaaattcaa
     5401 gttaaattta tattccatta cttctcctcc
//
LOCUS       DACENJ010000163         5413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.169, whole genome shotgun sequence.
ACCESSION   DACENJ010000163 DACENJ010000000
VERSION     DACENJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.169"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(243..677)
                     /locus_tag="IW748_002136"
     CDS             complement(243..677)
                     /locus_tag="IW748_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105042.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            825..1966
                     /locus_tag="IW748_002137"
                     /pseudo
     CDS             825..1966
                     /locus_tag="IW748_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2092..3171)
                     /locus_tag="IW748_002138"
     CDS             complement(2092..3171)
                     /locus_tag="IW748_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1105043.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3274..3714)
                     /locus_tag="IW748_002139"
     CDS             complement(3274..3714)
                     /locus_tag="IW748_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1105044.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3771..4181)
                     /locus_tag="IW748_002140"
     CDS             complement(3771..4181)
                     /locus_tag="IW748_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1105045.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4361..4795)
                     /locus_tag="IW748_002141"
     CDS             complement(4361..4795)
                     /locus_tag="IW748_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1105046.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4895..>5413)
                     /locus_tag="IW748_002142"
     CDS             complement(4895..>5413)
                     /locus_tag="IW748_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1105047.1"
                     /translation="GSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFT
                     DAFVVSLNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLR
                     LSAEIGRGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAE
                     VMGRGFTYHPIE"
BASE COUNT         1958 a         1006 c          904 g         1545 t
ORIGIN      
        1 ttacatattt ttaatagaaa atataattta tctatcatac cgctatttag tcggtcaagt
       61 acttcttttt taaaaaaagt attcatcata aaagccacta cttgcggatc ttcatcataa
      121 aattctgtag cagtacttga aaagatgttc caataaatat ctaatgctgc atattgaatt
      181 tgtacctctt ttccagaaaa agactttctg ccattattag aaaaggagat agacaaaccg
      241 tactatgcta aaaatgcatt aatctttttc attttcgtat acaaataaga ggcactatag
      301 tttgtttgct gactgaaact gataatcgaa tttgtctgac agctgaacaa agaagtcaag
      361 atctgataac tgactgattc gttgcagtat cttccaaata gacgataata gcagtaagcc
      421 ggatcaattc cttcaaaatt caactttaaa aaaggttttt gcttatcaat atgcagttct
      481 gttagattga gacgctcaaa gtcttgttct aattctttga tgtatagtaa aaaatttgat
      541 tcagacatat ggaaatgatc tgctaaatat tgtgtagtaa taaagaaacg atgttcagat
      601 aggaaaagaa aaatatctaa ttttgtattg ctttttttct tcaagataaa cgttttaatc
      661 gcttcttgag tttgcatgaa agaggctcct ttttacttaa attgttaaaa cttgaaaata
      721 ttactgtttt tttcataaat ttagacaaaa aaacagtaaa catataggta gaatctctac
      781 tatcttattt cttgcataca aaaaaatcaa ggagtgagag agttatgaga tattcaacca
      841 tcttaatatc aacatttgca gccttaagct tatcgcttac tataaatcag acagtccaca
      901 cagaggagac agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta
      961 cctttcagga tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa
     1021 cagcgaccat caaagcaaaa attgactatt cgggagactt agcacctata ctacaatcag
     1081 ctgttacatt tgagaatgtt gatcccactt tgtcgattgg ctatgacgag aacactttga
     1141 ctatccaaca caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa
     1201 aaccagcgct ttttactgtc aaggtatcag atggactacc gaacgatcat catttaacgc
     1261 cgtatagtca gcggcagaca attgagcaag attcagccat cgactcagga ggagatcttg
     1321 ttgaagaacc aacagataaa ccagaaaatg aaaataagcc agaagtaccg cctacagaaa
     1381 atccagacgg agaacagaaa ccggaaatag aacctggaga agaacccgat acagaaactc
     1441 aaccagagcc agacaataaa tcaaaacctg aaataacccc tggagaaaaa ccagatgtaa
     1501 ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat ccagacacag
     1561 ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc agaagaaaaa ccagatgtga
     1621 ctccagaacc agatacggac gcaagagacc aaggaatacc agaaaaaata aacaaaaaaa
     1681 caattcaaga ggatggaaaa aaagagagta aaaaatcaaa tctagcaatt ctaaaaataa
     1741 atgaagagca gctgaataaa aaatcacgaa tatttgatag cgcccaatca gctgaaacct
     1801 taaaaagtag caaggataca acctttgctg tcctgaaacg aaaaataaac agctaccaaa
     1861 atccggagag tcacaaaaca aagttatact gtggagcgga atcattctat taagtatagc
     1921 aacaatgctt tctgcaaaaa gatttaaaca aaacagatct ctctaacatt tgaataatgt
     1981 ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa atgaccccaa aataccagat
     2041 tgaaatctga tatttggggt cgttcatact ttatctttct tcatatttat tttaagggat
     2101 atctgtttct tttggaagat ggttcgggat ctttttcact ttcaatacat ctaaaaagaa
     2161 ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca aagaaccgtt cgctaacaag
     2221 taagaccaca aatgtataaa aaatcggcag ttctgttttg ctggacatca acttcggatt
     2281 cagcacataa gactcaatta gatggacaat caaaatcgta atcaggatat aaacaacatc
     2341 tcggatgcct ccttgggaat aagctatgaa agatagagga atacaagaaa tgataacacc
     2401 tgctactggg atcagactta aaatgaaaat catgatcgct aaactcagca gctgatgaaa
     2461 gccaaataca gccaaggcga ttgtagtgag caacgtattt aaaagagcga caacaaattg
     2521 agcttctaat accaaaccaa atgtattgac aaacttatcg gcaaaatagt aaatatcttg
     2581 aaagaaccaa gaaaattcac tttttaaaaa caatcttgag aatacaattg tttttttctt
     2641 ttcaatcata aaaaagaaac ttaagatgaa tgacatagca aacgccactg ccatttttcc
     2701 tatatcctgt aaatagccaa gcgcaatcga agcgccattt tgaagctgtt caaaaaaatc
     2761 gtttcgctca atataagtat ggatatattg taaaacagga tctgcatctt ttggctgatg
     2821 ctcgtaaaaa ttaataacgg actggatcat gtcaaatgtt tgatgaataa gcatcgggat
     2881 atactttgtg aatgccagat agataaggta aacgatcagt gtataaatca tgatagctaa
     2941 aacacctgta gggattcgga caaatcgctg aatgatttga acaagatgaa tcatcaaata
     3001 agtaaagata aatgtgagca aaatggttgt aatcatactt ctaactaaga aaagaacaaa
     3061 aacgacaaat aataaaacag tgagacggcg tattttttca ttcgccaaaa aacgctgata
     3121 aacggtcaaa tcgacaacct ccaaataaca aaaaataaac acgttattca ttatatgatg
     3181 gaatatgaaa ataagaaaga gcaaaaagaa aaaccgctaa atgacgttat cttatgagtt
     3241 ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt cttgatgaaa
     3301 attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct gttcaagtgg
     3361 aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg cagcaccttg
     3421 agcagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac gattaatata
     3481 atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca gacacaaatc
     3541 atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca tcacaagata
     3601 ctgggtatag gtaagattca gcggcttgag gaggggagtg tagtgtctaa tgatttcttt
     3661 agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt ccatttttcc
     3721 acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt ctattcacta
     3781 actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg acacacttta
     3841 tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat agatccttca
     3901 atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac ttgtgcactg
     3961 acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc actgttaaaa
     4021 catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc gacgcgttta
     4081 ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgcacttc tccagagcgt
     4141 ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaaattacc acctttaatt
     4201 atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa atggattggc
     4261 ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag caaacatata
     4321 caaaaaatat cagagacaaa aaataatctc atcataaaca ttacatatat ttctcaaaaa
     4381 gttgttttct ttcttcaaaa attacttcac gatggacctg atgaagcatc cataaataac
     4441 caaaaggatc tctgaacatt gagttagtca caccaaagtc catcatttct gtcatttctt
     4501 gaatctctac acagccattt cgtaatgctt tattatgtgt gtcctcgata tcagagacga
     4561 tgatattgaa ccagatcgta tttggatgtt cagcagtagg agcagtcaaa ccaagagacg
     4621 tgttttcatc cagcatatgg aatctcgtac catacaaggt aaaaatgact tcattattgc
     4681 ctctagggta atctgttgcc tcaatcagtt gtatttcatc aaagattgtt tgatacagtt
     4741 ctagtgcttt caaactatca tttaatacaa aatcgatttc cgttccttta tgcataaaga
     4801 tctcctctcc ttttcagtcg aacagttact agattctttt attataccag ctaaagaaaa
     4861 cgcattcaaa aaaatagcgg atttacttac caactcattc gattggatga taagtaaatc
     4921 cgcgtcccat tacttctgct acgctgctga caatgacaaa ggcacttgga tcgatttctt
     4981 ctatcaatgg tttcattcgg gaaaattcat gatcagtcac aacgatcaat aaaacctctt
     5041 ttgactttcg attatacccg ccttttgcct ctagcagggt aaggccacgt ccaatctcag
     5101 cacttaagcg aaggcggatg tcttccaaat aatgttcact catgaccata acagatttct
     5161 ttcgattgat acctgtttcg atataagtca tgacaattga agtgattgca atagataaaa
     5221 tagcataaag aaactcttct attccaaaaa taaacagatt aagagaaaca acgaatgcgt
     5281 ctgtaaaaaa taagccaatc gaagtattca agtggaaata ttttttaaaa atgaggggag
     5341 ggatcgtcgt accgccgcta gaagagttgt ttttatataa aatcgcaact cctaaagcaa
     5401 aaatcgcact tcc
//
LOCUS       DACENJ010000164         5412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.170, whole genome shotgun sequence.
ACCESSION   DACENJ010000164 DACENJ010000000
VERSION     DACENJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.170"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2187)
                     /gene="nrdD"
                     /locus_tag="IW748_002143"
     CDS             complement(<1..2187)
                     /gene="nrdD"
                     /locus_tag="IW748_002143"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1105048.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            2513..3280
                     /locus_tag="IW748_002144"
     CDS             2513..3280
                     /locus_tag="IW748_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1105049.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            3270..5312
                     /locus_tag="IW748_002145"
     CDS             3270..5312
                     /locus_tag="IW748_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1105050.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         1604 a          947 c         1047 g         1814 t
ORIGIN      
        1 tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca tcggacgagc
       61 ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt ttggatcatg
      121 atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc
      181 acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc
      241 aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt attcacagta
      301 gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca atttttcaaa
      361 tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt ctttatctgt
      421 gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt cagttagact
      481 ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc atgcatctgc
      541 attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc
      601 ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac
      661 agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa gacgtttccc
      721 gaaagcacca tacatatata aaattggcgc atttgctgga atggcttctt tacaacgttc
      781 tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta actgccagaa
      841 tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg taacaacacc
      901 taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc cttgcaagaa
      961 tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt tatcgtaatt
     1021 cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt tgatgtcgta
     1081 attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca gtttagggaa
     1141 gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa ttgctttttg
     1201 aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga aaggagtttg
     1261 tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag catcgaagat
     1321 atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat cgatccattc
     1381 ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa gcaattcatc
     1441 tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa taatctgtga
     1501 gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat tgccgatttt
     1561 gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca tcggtgtata
     1621 aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat ttgctatatg
     1681 tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca gatctcgctg
     1741 tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt ggtccttgtt
     1801 gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa aatctcgttt
     1861 ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta gtaatatatg
     1921 ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc ttttgatttc
     1981 ttggacaacc aagtctgcaa tcatttgaat tttttcataa gtaagtggtg ataatgagcc
     2041 gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac
     2101 atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct gagtaacgac
     2161 agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg ttcttgtcta
     2221 tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt attcaatgtg
     2281 ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa aaaggtgaaa
     2341 acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa ataattgaat
     2401 aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat tattcgtaga
     2461 tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa
     2521 gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc ccgtcaaagc
     2581 cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta tgggcccaag
     2641 tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc ctacgatggg
     2701 cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt
     2761 tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata ccctaaatgc
     2821 aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg aaatggaaaa
     2881 gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac ggtatcccga
     2941 tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg ttgttcagcc
     3001 acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg ctacagaatt
     3061 gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt tagtcaccca
     3121 tgatccatat acagcaagct actgcgatag aattttattt attaaagatg gtgtgatttt
     3181 ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa tagacatgca
     3241 ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt aaaagttttt
     3301 ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc gtaatgatct
     3361 attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca agtcaagata
     3421 cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt ttgctttttt
     3481 tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata ggcatctatc
     3541 agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa atcatgataa
     3601 ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc aaactctttt
     3661 caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt atctccattc
     3721 catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta tctgtatatt
     3781 caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa gaacaagcag
     3841 atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc gggttactac
     3901 ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca tggatgatca
     3961 gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt ttcgtttgtg
     4021 tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc atattgagta
     4081 aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg caagttcatt
     4141 tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt tctttaatga
     4201 caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa gtttcctccc
     4261 cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt ctagcagagg
     4321 aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg agtatctata
     4381 gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa acacaatatc
     4441 aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca cctggtcata
     4501 ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat ggatctagta
     4561 ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt gtattaggtg
     4621 acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt tttgaaaaag
     4681 caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa
     4741 tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt tattattctt
     4801 atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt ccggatgacc
     4861 aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct tccagctatt
     4921 cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg
     4981 ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta gcagaagcag
     5041 aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa agaaggatca
     5101 gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata ttagggatca
     5161 ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc gattactggt
     5221 tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat gtcattacga
     5281 gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag ataataaata
     5341 gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat ttttgtcaca
     5401 acctctttat tt
//
LOCUS       DACENJ010000165         5293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.171, whole genome shotgun sequence.
ACCESSION   DACENJ010000165 DACENJ010000000
VERSION     DACENJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.171"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            25..339
                     /locus_tag="IW748_002146"
     CDS             25..339
                     /locus_tag="IW748_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1105051.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            360..737
                     /locus_tag="IW748_002147"
     CDS             360..737
                     /locus_tag="IW748_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1105052.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            747..1520
                     /locus_tag="IW748_002148"
     CDS             747..1520
                     /locus_tag="IW748_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105053.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1542..2945
                     /locus_tag="IW748_002149"
     CDS             1542..2945
                     /locus_tag="IW748_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1105054.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3127..4311
                     /locus_tag="IW748_002150"
     CDS             3127..4311
                     /locus_tag="IW748_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1105055.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4308..4598
                     /locus_tag="IW748_002151"
     CDS             4308..4598
                     /locus_tag="IW748_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105056.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4595..4816
                     /locus_tag="IW748_002152"
     CDS             4595..4816
                     /locus_tag="IW748_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105057.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            4858..>5293
                     /locus_tag="IW748_002153"
     CDS             4858..>5293
                     /locus_tag="IW748_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105058.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNG"
BASE COUNT         1775 a          894 c         1065 g         1559 t
ORIGIN      
        1 aaaacagaaa ggataggtaa aaatatggaa cttaaatatg tcattcccaa tatggaaaaa
       61 acatttggac aattagaata tgctggtgaa gggaacattg aacaacgtcg aataaatggc
      121 agaccaacca ttctctctcg tagttacaat ttatactcaa cgattcaacg agcagatgat
      181 attgttgtca ttcttccctc cgaagctggc gagaaacatt ttgaatccga cgaaaaagtc
      241 aaactcatca acccacatat taccgcagaa ggttataaaa ttggcaatcg aggatttacc
      301 aattacatta tgcacgcaga cgatatggtg aaagcataag aaaggaaagg tgaaaaaata
      361 tgagattagc agaaggtatt gtgattgaaa aagaccagac atttggcaca ttgaaatttt
      421 ccgccttgcg tcgtgaggtt cgtatccaga atgaagatgg tacggtttcc gaagaaatta
      481 aagaacgtac ctatgactta aaatcaaaag gacaaggacg catgatacaa gtcagtatcc
      541 ccgcttctgt tcctttgaaa gaatttgact acaacgcaga agtggaatta atcaatcctg
      601 tggcagatac cgtagcgacg gctacttttc gaggggcaga cgttgattgg tacgtgaaag
      661 cagacgatct tgtattaaaa ggacagacaa aacctcatca gaaatataaa aaagaagcgt
      721 ccgaaagtga aaaataggtg ttgcttatga caaacacacc attgattttg aaagaaattc
      781 cgaaggatga agccatttct tttattcggc aataccatta ttcaaagata ctccctagat
      841 tgtgtaaata ctttctaggg attttttcag aagaaaaatt attaggcgtt gtggaattag
      901 gctggggtac gcaaccccta caaaccatac gcaaactctt tcccgacagt tcccttcaaa
      961 ccacagatta tttagaaatt ggaaagatgt gttttcttcc agaaatgaat caaaccaatt
     1021 attttggcag tcaagctctt tcagctctaa ttaagtggtt gaaagaacat acggactgtc
     1081 attttttata tacactggca gatggaattg agggaaaatg tggctatgtc tatcaagcgt
     1141 ccaacttctt ttactgcggg tatttcaaga caagtgtcta tcgtgataag cagtcatggg
     1201 aaaagataca ccctcgaagt gctcgccttt tattagagga aaatgctcgc tttgaacagg
     1261 tagagaaaaa gcattggctt tcccaggcat tttgcgaata caaagggatt gagaaaatca
     1321 atggacgcat gtttcgctat ctctatccgt tgacaaaaga agcgaaaaaa ctgttaggac
     1381 acactctcta tcgacgtcat tattatccga aagaaaaaaa tttacgcttt gaaaagcgaa
     1441 tcgcttatca gaaatatgaa gccatttctc aaccaacttt tgataaacag gctcgtatct
     1501 acaatacaca attattttga taggtggaaa ggaggatttt tatgccattg cttcatgaca
     1561 gaggagagcg aatcaaagcc aacgataagc acttggtcta tcattttgta ctaatttcat
     1621 tgctggtagt ttttctagtc gtcctactcc cttttcattg gagagcgtta caatcggtga
     1681 actggcaagc agttcagtta gatacatttt tctatcaact tcatacgcca tacttttgga
     1741 gcagtattct cactgctctt tttgtatgcg gacttatcag ctggctttat tatcactatc
     1801 gtatagattc tataaaacag ttagagcacc gtcaaaagtt agctcgtatg attttagaaa
     1861 atcattggta tgaagccgaa gaaactcaat cagagggatt ctttaaagat ttacccagca
     1921 agaaaaccaa acaacgaatc cgccattttc ctaaaatata ttatcgaatg aaagatggac
     1981 taatccatat tcatgtagaa atcaccttag ggaaatatca agatcagctt ttgcacctag
     2041 aaaacaaact agaaacagga ttgtattgtg aattagtgga aaaaacgttg aaagattctt
     2101 atgtagagta tgtcctactt tatgatacta ttggcaatcg aattggcatt gaagacgtgg
     2161 tagctcaaga tggacgtgtc aaattgatgg aatcggtcta ttgggagttc gattctctcc
     2221 ctcacatgtt aattgctggg ggcactggta gtggaaaatc ctattttatt ctcacattga
     2281 ttgaatctct cctgcataca aatgcaaagc tttatattct tgaccctaaa aatgctgatt
     2341 tggcagactt aggggcggtc atgaataatg tctattaccg taaagaagat atgttggcat
     2401 gtatcaatca attttacgaa gatatgattg atcgcagtga aaccatgaaa caacacccta
     2461 actataaaac aggagaaaat tatgcttatc ttggtttgtc tgccaatttt cttatttttg
     2521 atgaatatgt ggcaatgatg gatatgctag gtcgtgaaag taccaccgtt attcataaac
     2581 tcaaacaaat tgtaatgtta ggacgacaag caggcttctt tttaattctt gcctgtcaac
     2641 gtccagacgc aaaatatttt agtgatggga ttcgggacaa ctttaacttc cgtgtggctt
     2701 tgggtcgtat gtctgaaatg ggctttggta tgatgttcgg aagcgataca caaaaacaat
     2761 ttttcttaaa acccatcaag ggaagaggtt acgtggatac aggaaaaagt gtgattagtg
     2821 agttttatac accccttgta ccaaaaaggt acgatttctt aggagaaata ggaaaagtca
     2881 ttcaaaaaaa gcaatctgag cctgtaccgc acgaagtgaa aggtacaggt tcagacttgc
     2941 tttgattggt gtggcgttag ccacgccaat cctttagccc cctctatcta acaggggggc
     3001 acaaatacaa ctgaaaacag gcaattctca tcaaccaatg ttgatggtac aaaggattgc
     3061 ggaaaatatg aaaggtgtaa actcaagggt catagtttac atctttttta gattggaggt
     3121 ttgcgaatgg aaaacgaaaa aatttggatt aaggaattga aagaaaagcg actggcttat
     3181 ggcgtttctc aaaataaact ggcggttgct tctcatatta ccagacctta cctatcggat
     3241 attgaaacag gcaaggcagt ccctactcaa caggtgaaag aagatttact gaacgctttg
     3301 gaacgcttca atcccgataa cccattggag atgttattcg actatgtacg gattcgattc
     3361 cctaccaatg atgtggctca aattatcggt gaggttttac gtttgaatat ggattatatg
     3421 ctacacgaag actttggcta ttattcttat cccgaacact atcgtttcgg tgatattgtt
     3481 gtcctagtct ctcatgatgt aagtaaaggt gttttattag aactgaaagg aaaaggctgt
     3541 cggcaatttg aaaactttct attggctcaa catcggagct ggtatgattt ctttcaagat
     3601 tgcatggaac ataaaggtat tttcaaacgc ttggatttag ctatcaacga taaaacaggg
     3661 attttgaata ttccagattt ggcaaagaaa tgtaaacagg aagaatgcat ttccgtcttt
     3721 cgaagcttta aaaattatcg cagtggtgaa ttggttcacc gtgatgaaaa accatacatg
     3781 ggaaacacac tttatattgg ctcattaaaa agtgaagtct atttctgtat atatgagaaa
     3841 gattatgagc aatacgtgaa aaatgatgtt cccttagaag acgcagaagt aaagaatcgg
     3901 tttgaaattc ggttaaaaaa tgaccgagcc acccaagcca tgaaagattt attggcacat
     3961 caacaggcgg aaaaaacagc ctttgaaatt atcaatcgct atatccgctt tgcagataaa
     4021 gacgacacta aacgtcggag tgattggaaa actaatgaac gttgggaatg gtttatcggt
     4081 aaaaatcgtg gagcattacg cctaaccaca cagccagagc cttattcttt tgaacgaaca
     4141 ttgaactggt tacatcatca agtcgcccct acattaaaaa tcgcaagcat tttagatgtt
     4201 ctcaacggta caaccattat ctccaccatg attcaggaag ccaaactgac agaaaaacat
     4261 gagaaattaa ttgaacaaca acatctagcc atggaggatt tgattacatg agccttttaa
     4321 caaaagaatt aaagaagtta gggtttcagt gtggcattga gtttcaagcg tatatacaaa
     4381 atacagggaa atatacgtct ctcattattg agggaaaacg gcaagctggt gataccatct
     4441 atacctatga tttttacaag gtgagatttt ataataatta taccaatcga gtaaccgtct
     4501 atggagagca tttaacccct tttcaattat taaggcgtgt aaaaagctat atctattatc
     4561 gagaaaaata tcttaaggaa aggaggacaa tcacatgaat tttgggcaga atttgtacaa
     4621 ctggttcttg gataatgcac agtcattggt attgttagcg attgttgtca ttggactgtt
     4681 tttaggtttt aaacgagaat tttcaaaact cattggcttc ttgattattg ctctaatcgc
     4741 cgtcggactt gtgttcaatg cagctggcgt gaaagatgtg ttattaaact tatttaaccg
     4801 aatcattggt gcttaacaga tatttttcat gataaaatag gaagtatata ttttgttatg
     4861 aaaggattta tgaatatgtt tacaaatgat caatttaaat ctcttttaat taaaacatta
     4921 gaaagaaaga gctcttcaaa ttattatgaa ggaggtaacg agattgtttc taaaatgaaa
     4981 atatcagagg taactttaga tgagactgac gattttactt attataaagg atataaaaag
     5041 ggctggaata cgctttgcac atatcttaaa ataagagttc cttttgatga tttagacttt
     5101 tttgaaagtc ataaagattt gattacccag acagcaagct ccatatatga taaacaaggt
     5161 gataatgtat tagtggatac tattttagta ccacttccag agaattacga agtaattaac
     5221 tttagtcagt tgaaaattag tgatgtggtt tcacaagcta ttgaagacgc tgagtctttt
     5281 atgagtaatg gtg
//
LOCUS       DACENJ010000166         5240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.172, whole genome shotgun sequence.
ACCESSION   DACENJ010000166 DACENJ010000000
VERSION     DACENJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.172"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1773
                     /gene="acm"
                     /locus_tag="IW748_002154"
     CDS             <1..1773
                     /gene="acm"
                     /locus_tag="IW748_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1105059.1"
                     /translation="EIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFY
                     YKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQ
                     KVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQI
                     NFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIP
                     NVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSS
                     NELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEP
                     VPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLV
                     VTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFT
                     NLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPE
                     KTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSS
                     FAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2045..2530)
                     /locus_tag="IW748_002155"
     CDS             complement(2045..2530)
                     /locus_tag="IW748_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1105060.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3010..3651
                     /locus_tag="IW748_002156"
     CDS             3010..3651
                     /locus_tag="IW748_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1105061.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            3670..4305
                     /locus_tag="IW748_002157"
     CDS             3670..4305
                     /locus_tag="IW748_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1105062.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4323..5138
                     /locus_tag="IW748_002158"
     CDS             4323..5138
                     /locus_tag="IW748_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1105063.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1839 a          856 c         1077 g         1468 t
ORIGIN      
        1 gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc attcaccgtg
       61 acgagcggtg ataaaacagc tactgtaaat gtgacaaagc cagcttctgg ttcttcttct
      121 agcgtttttt attataaaac aggagacatg ctacctgaag atactaagca cattcgatgg
      181 tttttgaata ttaacaataa cggcacttat gttgaacaac cagttaaaat cagtgatgaa
      241 atccaaagtg gacaaagatt agatccaagt acgtttgaaa ttaatcaaat acacttaggt
      301 gaacagaagg tttatagagg agaagaggga atccaacaat ttttacaaga tttccccagc
      361 gctacattta atttttcagt aactgataat tacattgaaa taactatacc aaaaaacttt
      421 gtcaatctta ggaaaataat ggtgagttat aagacgatca tagaaaaccc agaacaaata
      481 aattttgaga atcattcgga agcttggttt aaagaattta ataaacctgc tgttgatgga
      541 gaaagtttca atcatacagt taaaaatatt tcagcttctg gtggagtcaa cggtacagtt
      601 agaggcgaat tgaagatttt caaatatatc aatgacacag agattggtat cccaaatgtg
      661 actttcgaat taagacgagc agatgaacag cctattcaag gacagtctag tatcctgttg
      721 acttcgaatg aacagggaga agctagtatc aaaggcctgc aagtaggaga ctatgttgta
      781 aaagaaaaag aagcacccaa ttggattgat tttgatccac taagttcaaa tgaactcaaa
      841 ttctccataa acgaaaatga tacagaggga gtcagtttac ctatttataa caagaaaaag
      901 gttacgaaca tcacagcaac taaaatttgg aacggtggaa ccactccaag accgagtatt
      961 tattttaaat tattcagagc aagtacaaac aattgggaac cagttcctga tgcagaaaca
     1021 aaacgattag acaatggaat aacttcagtg acttgggaag atatccaaca gtatgatgat
     1081 agtggaaacg aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc
     1141 ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc
     1201 atttcagtct caggacaaaa aacatgggaa gacaatgaga accaagacgg caaacgaccg
     1261 acgacgatca cagtaaatct attagccgac ggtcaaccga tccaacacaa agaagtaagt
     1321 gaaaaagatg attggcgcta tcgtttcaca aatctgccaa aatacagaga cgggcaagaa
     1381 atcatctata ctgtaacgga agacaacgtg cccgaataca gtacgacgat tgacgggtat
     1441 aacatcaaaa acagttatac accggaaaaa acaaatatat ctataccagg tagccatata
     1501 acaccgtgga aaaattttcc agagaagaca gagagagaga aaattagaaa tctattctct
     1561 caaaaattgc aaatatttgc tcatacagga gaatccagcg aacaaagaag caattatatt
     1621 agtaagagag ctgtacctaa acagataaca aataaatcaa aaagcattct tcctaaaaca
     1681 agtagtaaaa aatctagttt tgctgtaatc attggtttat tgatagtaac tatctgtgct
     1741 gggatattct tacaaaaata taaaaaagtt tagttagaat ataatatttt ttgttaaata
     1801 tgactgaaat caaaagaata aaattccgat ttataatcga aaaaagaagc atttttatat
     1861 atgcttcttt tttcgtagaa tcaataaata tggatttggc taactatgga agaatactcg
     1921 tattagtttt cagcgttcat actagttgtt acattgtttg attggccaaa agaatttctg
     1981 atatattttg tgctaaataa agaaaaagac gcttgaattg ggaaataaga aaactactct
     2041 atcattacaa attttcataa actggcagat gttctaagta aacaagtatc ttttcaaagt
     2101 cggaaaaatc cataatcaac gtagtggtat tgatatttgg atgaaagcct acggtatctt
     2161 gacgatcaat agcagcatcg atcacgactt ggattttttt ctctttgtcg tgcatcaatg
     2221 ctaatggagt cactgtacct gctggaacgc ctaggaggtc aggaagctgt tcttcagaaa
     2281 caaaagataa gcgcttttct tttaattgtt cagctagttg ttttaaatcg gcgtgctttt
     2341 catcaggcaa gatgacaaga tagatttgtt ttccttttct tgatttcaat aatagatttt
     2401 ttacttgcgg accaggtagg gaaaaaggga catttttgac tgacgtaatc gcaggatgat
     2461 cgactttttg aaaagagata cccagttttt ttagtagttc ataagcttct tgttcggttg
     2521 cacgactcat cgcgcatcac cctccttttt ttattagtgt atcataaaaa agagttgaca
     2581 aatgaaaaat aatcatgtaa attacacgta aagttaaaac aaatgacatc gaaaagaaga
     2641 gtagccgtta ggtgttttta gagagtctct ggttggtgga aagaggcagc agttaaccgt
     2701 gaaacacatc ttggagtcga ttttctgaag cgtatagtag gaaaatccgg ggagcccgtt
     2761 acagctgcgg tcgttgctag atgaccagat aaggctggct tttccgtgag gagtcggtaa
     2821 aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg gatcaaagga ctttttgttt
     2881 tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa ggaaaaaata aacagaggtg
     2941 gaaccacgta taatacgtcc tcttggcatt tgccaagagg atttttttat tttaaaggag
     3001 gaaaagaaga tggagtattt gattgaagtt atgccgcaat tattagtagg agcactcaca
     3061 acattgaaat tatttttatt taccttgatt ggctcattac cattagggat tgttttagca
     3121 ttaggattgt ctttgcattt taagccgcta cgctacttgc ttcaaacata tgtatgggtt
     3181 atgcggggaa caccgctgtt gttgcaactg atttttattt tttatgggtt gccgacgatt
     3241 gggatcgttt ttgatcgttt cgaagcagca ctcattgcct ttatcttaaa ctatgcagct
     3301 tattttgcag agatctttcg tggagggata caatcgattc ctcaaggtca atatgaagca
     3361 gcgcaagttt tgcaattaag tccctttcaa acggtgacga aaatcgtcat cccacaagtg
     3421 acaaagattg ttttaccttc tgtagggaat gaagttatca acttggtcaa agattcttcc
     3481 ttgatctata tattaggttt aggtgacgta atgcgtgctg ggaaaatcgc gatggaacgc
     3541 gacgcgactt tactgccttt gctaggcgtg gcaatcatct atcttacatt gactggtatt
     3601 ctaacaatcg taatgaaaca agttgaagga aaattaaatt attaccgcta actgatagga
     3661 ggaagaaata tgattgaatt gaagaaagtg aacaaagcat ttggtgaaaa aaaaataatc
     3721 aaagatttga atctaatcat tccagatggg aaaacattag ctatcgtagg tccttccggt
     3781 ggcggtaaaa ccacgctttt acgtatcttg gctggcttag aaagttctga cagcggagaa
     3841 ttttatttag acggtgtacc gttcaaccca ttcgagctta aaatgaaaga acaagttgta
     3901 ggagtcgtat tccaagactt ccagctattt ccacatctga atatttttga aaacattacc
     3961 ttagcaccaa aaatggtatt gaaacagaca aaagaagaat acaccaaaaa agcagaagaa
     4021 ttagcggctt cattagggat tagtgattta ttgaatatct atccttatca gctttctggc
     4081 ggacaaaaac agcgggtagc gattgccaga gcattggcta tgaatccacg ggtattggct
     4141 tatgatgaac caacaagtgc tttagatcct gaattacgcc agcaagtcga gcgtttgatc
     4201 ctatcattga aagaagatgg ggtaacccaa attattgtca cccatgattt agtatttgca
     4261 gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc attaagaaag gagaagatag
     4321 agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg tcttcttctt tttgcaggct
     4381 gttcgaacaa aaaaacttca gcagataatt gggcgactat caacgaagag aaaaaaatcg
     4441 ttataggttt agacgataca tttgttccta tgggatttag agataaagac gggaagctca
     4501 caggatttga tattgattta gccaaagcag tctttgaaca atatgggatt acggtagact
     4561 ttcagcccat tgactggtca atgaaggaat ttgaactgaa caatggaacg atcgatttga
     4621 tatggaacgg ctattccaaa acgccggcaa gagaaaagaa agtgcaattt acaaaacctt
     4681 atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc aggaatcaca aactttggac
     4741 aaatgaataa taagagatta ggtgcgcaaa atgggtcatc tggttatgat gtatttacga
     4801 aacaaccaga agtattaaaa gagatagtca aaaaccaaga tgctgttttg tatgatagtt
     4861 ttaatgaagc attgatcgat ctgaaaagca gacgggtcga tggtctgctg atggataaag
     4921 tatacgcaga ctattatttg aaacaaagaa acgaactaga agacttcaac ataacaaaag
     4981 gttcttatga gagtgaggat tttgcagttg gtgcacgcaa gtcagatagt acattagtga
     5041 aaaaaatcaa tgaagcccta gatcaactag agaaaacagg tgaattccaa aaaatatccg
     5101 ataaatggtt tggcgaagat gttacgccga gaaattaaca aataggggac tgtgacaaaa
     5161 gacgtgtcac agtccctttt tccgaataaa cagtgttctc ctgccaactc cttgtgactc
     5221 ggagctgaat ggcaggctca
//
LOCUS       DACENJ010000167         5199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.173, whole genome shotgun sequence.
ACCESSION   DACENJ010000167 DACENJ010000000
VERSION     DACENJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.173"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1868
                     /locus_tag="IW748_002159"
     CDS             <1..1868
                     /locus_tag="IW748_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1105064.1"
                     /translation="TPPEKVNITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTK
                     YNKADYGDVEFTLGNITDQVLPTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKIT
                     SAVDENGEITFADQPAYVNSKGNVYVIYESKSAAGLVTQKAKPMVVIAPMTDNTGSGF
                     LKDIHLYPKNIVSKLSFELTKFGDDGTEQSKQTPLKGAKFELYKGEPGKGSKLGDLVS
                     DDQGKLTATDLTLGKYYFVEVPSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITN
                     DGVTSDLKASYVNYKAPVIDKTVTNGTGQEHSFQVGDAVNYQGTIHIPTDIAGGADGI
                     TVNGVKSETSPYSVFKWGDTAGQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSEN
                     GFVIDFIVNNGQVSDTVASLHGQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQ
                     EEHHEKTKADVVTYGAKFLKVDSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDK
                     DGVKEAVWVDDVANAAILKSDKEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKV
                     DKDSYKEENRITIKNNQKASVPMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            1881..2633
                     /locus_tag="IW748_002160"
     CDS             1881..2633
                     /locus_tag="IW748_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1105065.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVSKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            2649..3404
                     /locus_tag="IW748_002161"
     CDS             2649..3404
                     /locus_tag="IW748_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105066.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLVSNGETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSEKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            3417..3677
                     /locus_tag="IW748_002162"
     CDS             3417..3677
                     /locus_tag="IW748_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105067.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            3674..>5199
                     /locus_tag="IW748_002163"
     CDS             3674..>5199
                     /locus_tag="IW748_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1105068.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRQDAVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     "
BASE COUNT         1893 a          868 c         1047 g         1391 t
ORIGIN      
        1 aaacgccacc agagaaggtc aatatcacgg ttcataaatt aatgtacgat caaagtacgc
       61 aattgaatgt tgatattgac gggatcaaaa atgatggtta tacgcacgat cagtatccta
      121 caggtgtaac gaaatacaac aaggcagatt atggtgatgt tgagtttacg ttaggaaata
      181 ttacggatca agtattaccg actgaagatt ctgatctaac caacgcgaaa gtggacgaaa
      241 tcgtgaaaga cgtagaagac aagggatcta acagtgagta cgtcaagaat gcaaccaata
      301 aaattacttc agctgtagat gaaaatggtg agattacttt tgcggatcaa ccagcgtacg
      361 tgaactcaaa aggaaatgtg tatgttattt acgagagtaa gtccgcagca ggattagtaa
      421 ctcaaaaagc aaaaccaatg gtggtcattg ctccaatgac tgacaacaca ggttcaggtt
      481 ttttaaaaga cattcactta tatcctaaaa atattgtttc taagttgagt tttgaattaa
      541 ccaaattcgg tgatgatggt acagagcaaa gcaaacaaac ccctcttaaa ggcgctaaat
      601 tcgaattgta taaaggagaa cctggaaaag gaagcaaact aggcgatctt gtttcggatg
      661 atcaaggaaa gctgactgct actgatttga cgttaggaaa atactatttt gtggaagttc
      721 cttcagaagt cgtagtggga tcagataaag aaccaacagc cgatcaatat ttattaggcg
      781 cagacgctcg caacgatgcg cacaataaat taacctttga aattacgaat gatggcgtga
      841 caagtgactt aaaagcttca tacgtaaact ataaagcacc agtgatcgat aaaacagtaa
      901 caaatggaac aggacaagaa cattctttcc aagttggtga tgccgttaat tatcaaggaa
      961 cgattcatat cccaacagac attgcaggcg gagcagacgg tatcacagta aacggcgtga
     1021 aaagcgaaac ttctccttac agtgtattta aatgggggga tacagcagga caaggacttt
     1081 cttatgtcgc tgctaaagca aatatcaaag taacaaataa agatggatca gtggtattaa
     1141 aagaaaatac agattataag atccaaaatt cagaaaatgg ctttgtgatc gattttatcg
     1201 taaataatgg ccaagtaagt gatactgtag caagtttaca cggacaagat cttcaaatga
     1261 cttataacat gtatgtaaat gacagcgcag ctgttgccaa tccattaaca aatagcgtgg
     1321 atttcgttta taacaacaat ccatttaatc aggaagaaca ccacgaaaaa acgaaagctg
     1381 acgttgtgac ttatggcgcg aaattcttaa aagtcgatag cggactattt ggaacaggaa
     1441 ttaaggcaac tcctcttgaa ggcgcagaat ttgcagctaa gaatgcagaa ggtaagtact
     1501 acggtggact tactgataca gataaagatg gtgtcaaaga agcagtatgg gtagacgatg
     1561 tagcaaatgc agcaatccta aaatcagata aagagggtca ttttgaaatc actggtttaa
     1621 ctgaaggaga ttactcactg gaagaaacaa aagctcctga aaactatcaa aaattaacaa
     1681 aagagatctc tttcaaagta gacaaagatt catacaaaga agaaaaccga atcacgatca
     1741 aaaataatca aaaagcttcc gtaccaatga ctggtagcaa cggtttccaa tcttacgtat
     1801 tgatctcttg cttgttgctt ggggcaggcg caatttctgc agttgtttac tttaagaaga
     1861 aagcctagaa ggatcagcta atgattatca gacaccctaa gaaaaagcga atcatgggta
     1921 aatggatcat tgctttctgg ttactttcag ctgttggtgt cttacttttg atgcctgctg
     1981 aagcgagcgt ggcgaaatac caacaaaatc agcagattgc cgcaatcgat cgaacaggaa
     2041 cggcagcaga aacggattct tctttggatg tttctaagat tgagttaggc gatcctgttg
     2101 gtattcttac aattccaagt atttctttaa aactaccaat ttatgacgga acttccgaca
     2161 aaattttaga gaatggtgta gggattacgg aaggaactgg cgatattaca ggaggaaacg
     2221 gtaaaaatcc tttaatcgca gggcacagtg gactttataa agataatctt ttcgatgatt
     2281 tgccttcagt taaaaaggga gaaaagtttt atatcaaagt tgatggtgag cagcacgctt
     2341 atcaaatcga tcggattgag gaagtgcaaa aagacgaact acaaagaaac tttgtcactt
     2401 acttagaacc aaatcccaat gaagatcgag taacgctaat gacatgtacg cctaagggga
     2461 tcaacacaca ccgtttccta gtgtatggga agcgagtaac ctttacaaaa tcagaattaa
     2521 aagatgaaga aaataagaaa caaaaattat cttggaaatg gcttttagga agtactgttt
     2581 ttctttcagt catgatcatt ggttcgctat ttgtttataa aaagaaaaaa taaaggaggg
     2641 taaccaacat gagaaataga aaatggctat ttgtttgtgc agccgctctt ttttctttca
     2701 ctgcctacac acagttgacg tttgcagaca gtcaagaaag cacggatagc acagaacaaa
     2761 caatccaaac gtcagatagc agcacgattc aaagcagcga tgaaaccgtc gattcgtcaa
     2821 cagtggattc ttcagaagaa ccgaaaattg ctgaagacga tcaaatagtc aaacagacca
     2881 ttcacgttca aaagattatt tcttcagaac aattagacaa ccaggggcat gaaaaacaag
     2941 aaggagtaaa tggcgcgaag ttttctgttt atgatgtatc ggacatttta caaaagatgg
     3001 atgttaagga tctcactact gaccaaattg aatcccaatt aaaagatcga gtgaaaaaac
     3061 tatccagtga tcaattgaag ctagtttcta atggagaaac aaagacaatt gatcaacaaa
     3121 caggtgtctt tgaattctcg gtagaagtac aggcaaatca gaaacaagct tattatattg
     3181 tcaatgaatc aagtcctgaa aacatttcta atagcgagga tattttgcta ttaacacctg
     3241 tttctgaaaa aaacggtgaa ttcttaaagg atgtgtggat ttatcctaaa tcagaagctt
     3301 cgcaacctaa agaagaagta aaaaaaatcg tttctactgg tgtaaagaaa aacttctttg
     3361 agaattgttg ggattttgtt actcacttgt tttctagaga ttaaggaaga aataagatga
     3421 aactgatcaa attattatta ttcaatcttt cttgtttaac cgcagtcttt ttgtactgcg
     3481 gtttattatt ttctcaatta tctattttgc caagctacca actataccta atgaggattt
     3541 taaacatacc agctgcttcc atgccgctag atattatgga aaagctcatt aggaatccat
     3601 tatttactac gatcaatttg atcgttattg tcggaatttc actagtcatt tataaaaaag
     3661 aggggatgaa gggatgaatt ttaaacactc gtggaccaga cggttaaaat atgtttttcc
     3721 cataatgatg atgttaggta cattgtttgg gttaaaaaca gccaatgtct ttgctgaaaa
     3781 agttattgtc gatccgtcaa atccaatcca gaacgtaaag aataatttta ttattccaaa
     3841 agatgttcct aatgcaaaaa caaatattac tgtaaacgga gcgtccaaag tttacaaata
     3901 tcatgttgat gccaacaaag gtggttttac agacgactac gtaggattag tggagggaaa
     3961 caaaaatata cgctatcctt ctttccaaaa ggaatacggt gaaacaaacc ttgttttaga
     4021 aggcgtaaca accaacacca aagtcaatat tgactatgga aaaatcggta cgtataacgg
     4081 gaaaaaagtc aatatcaagc tagttctatc taatattcac ttatactcgg atacgttgcc
     4141 gtggaatcta ctagataaca actatacaaa aactcatttt cgagacgatg gctataaaaa
     4201 tacgaatggt gcaatgagta aatctaaaaa aagaacagta ctttggatct cagacaatct
     4261 tttctcagga attgtttacc attcaacaca aatgaatgtg cagctagtag ccacatatga
     4321 ggacggtagc cccgttcaat tttcaggaga tacatttatc agttttaact ctttgaaccc
     4381 tgcaggagga aaaagcacgg atcttaaagg agaatatgcc cactatgaca aaatgaatac
     4441 gacagattgg tatgtcagac aagatgcggt tctttcagaa tttaaatctt tctacaacaa
     4501 tctgaacgtg gtaggtggcc acccaggagg aagctctaag ctgacacaag cggataatga
     4561 tttcaataat ttgcatgata aattaggaga tccgaagttt ggtcaaggta ctgtatcctt
     4621 taaaatatct gaagcaaatc cgacgtttgt gattggttct tctaatgttc aaacctggtt
     4681 tactttatct tctgcgacaa tctttagtgt tgttcctgat caacctgaaa aaacaggggt
     4741 tgataaaaat ggaaataacg tgaacgataa aatgttgcaa gtaggagaca cgattcaata
     4801 tcgaatcaaa cagaaggtga atcgattagg agtggattta ctagcggttt acgatcgatt
     4861 tgagttaatc gataatttac caaaagaagt agattacgtc gatgcacacg ttgaaagtgg
     4921 tacaaataaa aaattcgatg tatctggtga agtgacctat gacaaaacaa aacaccaggt
     4981 aaaatatgca gcgaaagctg atacattaaa aaatcgaatg aagtataacg gcgaaactta
     5041 cgaattagtg atcaatgtaa aagtcaatga attggccaat caaaatagtg tagccaaaaa
     5101 tcagggaacg tcgatcatta ataaagtaga aaaggataca aatatcatta ccgtttattt
     5161 ccctaaaatt ccagtgaaag aagtacaaca aaatggaaa
//
LOCUS       DACENJ010000168         5117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.174, whole genome shotgun sequence.
ACCESSION   DACENJ010000168 DACENJ010000000
VERSION     DACENJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.174"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            129..1118
                     /locus_tag="IW748_002164"
     CDS             129..1118
                     /locus_tag="IW748_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ1105069.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            1407..2105
                     /locus_tag="IW748_002165"
     CDS             1407..2105
                     /locus_tag="IW748_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ1105070.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            2255..3571
                     /locus_tag="IW748_002166"
     CDS             2255..3571
                     /locus_tag="IW748_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1105071.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            3588..5003
                     /locus_tag="IW748_002167"
     CDS             3588..5003
                     /locus_tag="IW748_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1105072.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
BASE COUNT         1689 a          809 c         1104 g         1515 t
ORIGIN      
        1 agtaaaaaac aaatggcaaa aaaatatacg aaaaaatcat tttgattaac tccttttctt
       61 taaaatgtag taaactaaaa atagaaaaaa gaaagcgttt taaaaagatt tgatgaggag
      121 gagaaaaatt gtatattgca gatagtgcac gttatgaaaa aatggaatat cgtaaatccg
      181 gtcattcagg tttgaaatta ccgattttat ctctcggatt atggcaaaat tttggtgatt
      241 atgatcctat ccataatcaa agagagattt taagaggcgc attcgacatg ggaattaccc
      301 atttcgacct tgcgaataat tatggaggcc ctgctggagc tgcagagaaa aactttgggc
      361 gtatttttag agaagatttt caagcctacc gtgatgaact gattatttct agcaaagcag
      421 gttatcatat gtgggaaggg ccatatgggg aatggggctc tcgtaaatcc atcatttcaa
      481 gttgtgacca aagtctccaa agaatggggc tagattatgt tgacatcttc tatcatcatc
      541 gcccagaccc tgatacacct ttggaagaaa cagcagaagc tttgatgcaa cttgtaagac
      601 aaggaaaagc attatatatt ggtatatcca actataatgg agaagataca aaaaaaatga
      661 ctgaaatttt aaaaagaaag gaagcaccgt tcattattca tcagatgcgc tacaatatgt
      721 tttctcgtgc actactagaa gacgatctat ctccagtatt agaagaagaa gggctagggg
      781 caatcacgtt cagtccgctt gctcaagggt tgctgacaaa tcgttatcta catggtattc
      841 ctgaagattc tagagcacat cgtaaggaaa taccattcct ttcagaagaa caagttggat
      901 caactctgga aaaaatcaaa gcactgcaaa caatcgccgt atcacgagga caatcattag
      961 cgcaaatggc ccttgcatgg aatttgcgac aaaaatctgt tacaagtgta ctggtcggag
     1021 caagtcggtt aagtcagtta caagaaagtg ttcgaatgat ggataattta gatttctcac
     1081 cagaagaaga attgagaatc gaccaaattc tagaatagaa acgatgtaat caaacttttt
     1141 atccatatat taaaaaaaac aaaagatgac atcaagaatc gaatgaagat cgtctgttat
     1201 tgaacatcat attttttgtc gttcaaaata aaaatgatcg aaggctagga gtgaatttta
     1261 tcctagtctt ttttatggta aatatcttct tcgtttgtat gtctatgaac ggattaatca
     1321 attttgtata caaaaaatca tcagatgatt caaacgtgtt ataataatta cgaaaaaata
     1381 catcagatga aaaggaggta tgtggtttgg aagaaaataa aaccctagta gaagtaacta
     1441 ctgacaagat ccttagatat ttaaaggaaa tgcagttgca agtaggagat aaactgccga
     1501 atgaatatga attgtccgat tatttagagg tggggcgaag cacggtgcga gaagctgtgc
     1561 gggctctagc ttccaggaat atccttgaag ttcggcaagg agcggggaca tttgtcagtg
     1621 ataagcgagg gattgccaat gacccactcg gtttttcttt gattgaagat caggatcgga
     1681 tgatacgaga cttatttgaa ctgagatatt tattggagcc taagatggca ggtttggcag
     1741 cggaatatgc aacgaagaat caaatcgtcc aaatgaaaaa attaacgcaa aagattgaaa
     1801 cttcttttcg agaaggcaca caagaacatg ttcaattaga catcgatttt catacgatga
     1861 ttgcagaagc tagcggaaac gtagccttgc tgcatattat cccaatcatt aatgaatcaa
     1921 ttgctttgtt taataaaaat tatgatatcc agcatctgaa aatcgagacg atcgatattc
     1981 atacagagat cgttcaggcc atcgaaaggc ggaatgcact tgctgcgttt gatgcaatga
     2041 ctgtccatat ggcaataaat cggagagaat tacagaaaat aataggagaa aaagaaacta
     2101 attgacagtt catctgatgt attttatgat aaatacatca gatgaatttt tgttttatat
     2161 ttggtaaaaa agaggagtaa atagtttctg ttcatcgaaa tgtaagcgct gactaataga
     2221 caaaaagaga tgacgtcaag gagaagaagc aggaatgagg ggatctggaa tgagttttat
     2281 ggataaatta gccaatgttt tagggaacgt ggcaacgaga atcaataatt taaggtatat
     2341 catggtgata aaaaacgcat ttgctgcatt gattccggta attattacgg gtgcttttgg
     2401 cacattgttt tcagcaatgg tctttgactc ggaaaatggc ttagcacaga taagtttctt
     2461 gcgtttttta gaagaattaa agcctatcgc atctgccatc agctatgtca cactaagttt
     2521 cttgaccatc tatgctgttt ttctgatagg gatcgaatta gccaagctga ataaagtgga
     2581 tggtgtattc cctggtatca tcgcagtgat gtcttattta tctgttaatc caactgttta
     2641 tgaattagtg aatgaaaatc agactgttat agtagaaaac gtattggcta aacaatatac
     2701 agatacgaaa gggttatttt tgggaatgtt ggtctccatc ctttctattg aactttattg
     2761 ctggttgagg aaacagaaga aactacagct gagaatgcct gataccgtgc caacgaatgt
     2821 ttctgctagt ttttctgctt tattcccgac gattctgact gtatcgggta tcgcggctgc
     2881 gggatttatc atcaaagaac ttacaggaat gtacctttat gatatcattt acaatgtcgt
     2941 acaacgccca ttagaaggtg tcgtacaagg gttaccgggt attttgctat tgatgtttat
     3001 tgcgcaagtc ttttgggtga ttggtatcca tggaaatcaa atggtgaagc cgatacggga
     3061 accgttgctt ttagcggcaa ttgctgtaaa tacggaagct tttgaagctg gaaaagaagt
     3121 accgaacatt attactatgc cattttggga tatgtatatg agtatgggcg ggtctggagt
     3181 cacgatcggt ttactgattg cagtattgtt agttggaaaa cgggaagata tgaaacaaat
     3241 caccaagtta tcccttgctc ctgggatctt caacatcaat gaaccagtta tttttggtat
     3301 gccgatcatg ctgaatccaa ttttagctat tccgtttatc ttaacgcctc ttatcactgg
     3361 aacgattggt tattttgcaa ccgctttagg atttgcggcg aaagcagttg taatggtccc
     3421 ttggccaatg ccgccgatcg tcaatgctta tctggcaaca gcaggaaata tcggtgcagt
     3481 tattacgcaa cttgtttgta ttattgtttc tattttgatc tatctgccat ttgtaaaagt
     3541 agcaaatcgt gtggaaaatg atgggatcta aaaaacagga ggaaatcatg aaaattacta
     3601 ttccaaaaaa ttttattctt ggtgctgcat cttctgcttg gcaaacggaa ggttggaaag
     3661 gaaaaaaaga aggacaggat tcgtttatag atagctggta taaaaatgaa cattttgttt
     3721 ggcataatgg gtatggccca gccgtagcaa ctaattttat ggaacaatac cagcaggatg
     3781 tggatctgat gaaatccatc catctgacac attatcggac ttctatcaat tgggctcgtt
     3841 tttttactga ttatgagaac tgtatagtag atgaagaata tgctcagcat ataagtgaaa
     3901 tgatcgactc tttgatcgaa gcagacgtag aaccgatgat ttgtttggaa cattatgaag
     3961 tcccagcttt tcttttggaa aaatatgatg gctggtcttc aagaaaagta gttgattttt
     4021 atgctaagta tgctcaaatt gcctttgatc gttacggtga tagggtaaaa aactggttta
     4081 cattcaacga accaatcgtt ccccaaacgc gttgctattt ggatgctgtt cgctggccgc
     4141 atgaacaaaa cacaaaaaag tggatgatgt ggaattatca taaagtttta gcaacggcta
     4201 aagcagtaga gatttaccgc agcggaaatt atggtggaag gattggctgt attttaaatc
     4261 ctgaaatggt ctatgctcga tccaattctc cagaagacca aaaagctgct tatatgtatg
     4321 atttgtttta taatcgagta ttttttgatc cgatggtaaa aggatactac acagaagagt
     4381 tcctggagtt gtgtcaaaaa caatcgattt atttcgatcc tgcaacagag gatttagaaa
     4441 cgattcagca tcatacagta gatttccttg gaatcaatca atattatcct aaacgcgttc
     4501 gcgcccctcg ttatcaatgg aatgaagaga cacctttcca tcctgaacga tattatgaag
     4561 tctttgattt acctgggaag aaaatgaata attccagggg atgggaaatc tatccgaaaa
     4621 tcatgtatga tatagccatg tatctaaaag aaaattatca taatatccct tggctgataa
     4681 cagaaaacgg tatgggaaga gaatatgaag aacagtatat ggatgagaaa ggaatcgtgc
     4741 aagatgatta ccgtatcgaa tttatcggac agcatttgca ttacttgatc aaagcaacag
     4801 aagaaggcgc aaattgcgaa ggctatatgt tatgggcatt cactgactgt gtgtcaccaa
     4861 tgaatgcatt taagaatcga tatggattag ttcgaatcga attgaacgat gaacgaagca
     4921 gaacattgaa aaaatctgct tcgtggtatc agactttgat tgataagaga gaattggatt
     4981 tgcctgacga aatctataaa taaatagaag ttttttgtag atgttcattg tatttactgg
     5041 ttatttgaaa taaaagaggt tgtgaatcca gcgttttgag ctaataccca gggtcagctg
     5101 attgaacacc gtttatt
//
LOCUS       DACENJ010000169         5100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.175, whole genome shotgun sequence.
ACCESSION   DACENJ010000169 DACENJ010000000
VERSION     DACENJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.175"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            76..1953
                     /gene="tdc"
                     /locus_tag="IW748_002168"
     CDS             76..1953
                     /gene="tdc"
                     /locus_tag="IW748_002168"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1105073.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            2196..3617
                     /locus_tag="IW748_002169"
     CDS             2196..3617
                     /locus_tag="IW748_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1105074.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            3726..5094
                     /gene="nhaC"
                     /locus_tag="IW748_002170"
                     /pseudo
     CDS             3726..5094
                     /gene="nhaC"
                     /locus_tag="IW748_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
BASE COUNT         1521 a          937 c         1095 g         1547 t
ORIGIN      
        1 tagtttacag aatagaagaa caggtgtaat gtagggctcg acaaaattta aatatttttt
       61 taggagggtt tcactatgag tgaatcattg tcgaaagatc taaacctaaa tgccctattc
      121 attggggaca aagctgagaa cggccaaatc tataaagctt tgttgaatga attggtggat
      181 gaacatttag gctggcgtca aaactacatg cctcaagaca tgcctattat tacgccagaa
      241 gaaaaaagca gtgctagttt tgaacacact gtgaataaaa caaaggatgt tctatcagaa
      301 atttcagcgc ggatgcgtac ccattccgtt ccttggcata atgccggacg ttactggggt
      361 catatgaact ctgaaacatt gatgccatct ttactagcgt acaattttgc tatgctatgg
      421 aatggtaata acgttgccta tgaatcttct cctgcaacaa gccaaatgga agaagaagta
      481 ggaatggaat ttgcaaaatt aatgagctat aaagatggct ggggccacat cgttgctgat
      541 ggttctttag caaacttaga aggactttgg tatgcccgca acatcaaatc attgccgctt
      601 gcaatgaaag aagtgacacc tgaactagtt gctggaaaaa gcgattggga attaatgaac
      661 ttgtcaacag aagaaatcat gaatctgtta gacagcgtcc cagaaaaaat cgacgaaatc
      721 aaagctcact cagcacgtag cggaaaacat ttagaaaaat taggaaaatg gttggttcca
      781 caaactaaac actattcatg gctaaaagct gccgacatca tcggtatcgg tttagaccaa
      841 gtgattccag tgccagttga ccacaactat cgcatggaca tcaacgaact tgaaaaaatc
      901 gttcgtggtc tagcagctga aaaaacacca atcctaggtg tagtcggtgt tgtaggatca
      961 acagaagaag gagcaatcga cggtatcgat aaaatcgttg cattgcgtcg cgtattagaa
     1021 aaagacggta tttacttcta cttacacgta gatgctgctt atggcggata cggccgtgcg
     1081 attttcttag acgaagacaa caacttcatt ccatttgaag atttaaaaga tgttcattac
     1141 aaatacaatg tctttacaga aaacaaagat tatatcttag aagaagtaca cagtgcatat
     1201 aaagcaatcg aagaagcaga atctgtaacg attgacccac ataaaatggg ctatgttcca
     1261 tattctgccg gtggtatcgt catcaaagat atccgcatga gagacgttat ctcttacttt
     1321 gcgacatatg tatttgaaaa aggcgcagat atcccagcat tacttggggc ttatatcttg
     1381 gaaggttcaa aagcaggggc aacagcagcc agcgtttggg cagcacacca tgtcttacct
     1441 ttgaatgtca caggatatgg taaattaatg ggtgcatcaa tcgaaggtgc acatcgtttc
     1501 tacaacttct tgaatgactt gtcatttaaa gtaggagaca aagaaatcga agttcatcca
     1561 ttaacttatc cagatttcaa tatggtagac tatgtattca aagaaaaagg caacgatgac
     1621 ttagttgcaa tgaataaatt gaaccatgac gtatatgatt attcttcata cgtgaaagga
     1681 agcatttacg gtaatgagtt cttaacttca catactgact ttgctatccc agactatggc
     1741 aacagcccat tacaatttgt gaaccaacta ggattctcag atgaagaatg gaaccgtgcc
     1801 ggaaaagtaa ctgtattgcg tgccagcgta atgacaccat acatgaacaa agaagaacac
     1861 tttgaagaat atgctgaaaa aatcaaagca gctcttcaag aaaaattgga aaaaatctac
     1921 gcagaccaat tattggcaag cgaagcaaaa taagcaaggt aatcatgtta gaaaacagct
     1981 tgaatactaa ctgtttctga cgcatgtttg gatctgaaga gcaaggggag acctaagctc
     2041 ttcgatccct ttgttttgag gttgtctttg aagaaaccct tcaagcaata aactcaaaga
     2101 atcccctcaa atattcgctg aattgtttcg tttattgctg aaactacttc aaagacaccg
     2161 ttattttatc caaaaaatca aggagagtgt caacggtgag taaatctgaa gtaagtaaaa
     2221 tgggtcttgg gacctttatc ggtctgacaa tggcactgtg tgcaacggtt cggagtattc
     2281 ctacgttagc cgcagtcggt tggacattga ttttttattc aatttttgca gttttgtttt
     2341 ttgccggtcc gatatcaatg gtttccggtg agctttccac gatgcttcct caagaaggcg
     2401 gtcctcagtt atgggtaaaa acagcactgg gcagcaaatg gggatttgtc gttgcatggc
     2461 tattatgggt gcaaatgttc ccaggtatgg taatggtcgc ttctacttta ggaccattgt
     2521 taggaaacac atttggtaat gtagaacttg gcaataatca ctggttcgtt ttaggttgta
     2581 ttttagttat ctactggatc atcacgatct tgaacttgaa attcgatatg gcaaaagtcg
     2641 gcgggaacat cggtgtatgg ctaggggtat atatcccagt agtcatcatg ttcgttttag
     2701 gtgttttggc agccttcaaa gtcggactag tctcaaacgg ctatctaggt gacttttcat
     2761 ggtcaaaagc tttccctgac ttggaacata ttgattcatt gaaatactta gctggtatca
     2821 catttatttt tgttgggatc gaaatgagtt ctgtttacat gcctcgtttg aaagacgcaa
     2881 caaaaaatta tacaaaaggc gtatttatcg ctttgatcgg attagtttta ttgaatgtca
     2941 tcaatgcgat gcttgtagca aatgtcgtac cagatggaaa aatggaatta gcaaatatca
     3001 cgcagcctat cttgattgac tgtcaaatcc taggcttacc agaagtgatc ggaaatatct
     3061 tcagcttcat ggtatttatt ggtgtgcttt tacaattatc agcttgggtt actggaccat
     3121 caaaaacaat catccaagta gctcgtgaag gattccttcc accaaaattc ggattccata
     3181 aagaaaacaa atacggcgta tctagaaatg tcgttctaac acaatcaatc gttatttcat
     3241 tatttgcttt gctttatggt gtaatggatg acgtaagtgc tgtattcttg acacttacaa
     3301 atgctacaac tgttatttat tgtatcgtgt acattttgat tgctgtttca ttgcttaaaa
     3361 tgcgtaagaa acatccagaa ttcgaacgtc cttaccgaat tggtaaaaac ggaaacggat
     3421 tagcttgggt agtcagctgc atgctgatct tctctatcat cgttgtcgta tttgctacat
     3481 taggtacagc gacattgagt gatgcattat tagtagcagc tatcacagtg gtcatgttcg
     3541 ttattccatt gatcatcaat cacttcaaga aagattcttg ggggattgag gtagaaaaaa
     3601 gtttagaaga aaaataaaaa aataagtctt tcagccgacg ttttgtcgct gatttagtag
     3661 gaggtcctgc caagtgaata gttttttcat ggcagggcct tttcatttga aataggagat
     3721 cgacgatgaa taaaaatatt tctttaaaag aaagtatcgc tgtattgatc gtgttgttgg
     3781 cactcctagg agtactgatc atcggattcc agctctcgcc acatatcccg atccttgcag
     3841 ctttcatgtg tttgttgttt tatggcagat ttaaaaagtt cacatgggat gagatccatg
     3901 acgggatcat caaaggaatc actccaggga tcattccaat cctgattttt cttttgattg
     3961 gtgtcttagt tgcttcatgg attttatctg gaaccatccc tacgattatg gtttatggat
     4021 ttaaacttgt ttctgtgcgt ttctttttac cgacagcatt tcttgtttgt gcaattgtcg
     4081 gagtgactgt cggcagttca tttacaacga tttcgacgat tggaattgct tttttgggga
     4141 tcggtcattt acttggattc aatgatgcaa tgactacagg cgcagtcgtt tccggtgcct
     4201 ttttagggaa taactgctcg cctttatcag atacgaccaa cttggcagcg ggtattggag
     4261 gagttaatct attcgaacat atcgctggta tgcgttatac ggatttccca gcattcgtcc
     4321 tttcgttgat tttttatacg atattaggac ataacagtca ttcagccgat ctagctagta
     4381 tcaatgaaat gatccaaaac atgaaagcga gcttttggat ctctccttgg acattgattc
     4441 cactagttat tttatttggg gggggcaatc aaaaaaattc cggctatccc tacattacta
     4501 gcgggatcga cagctgctct cgttctttct tttatccatg agaaaagttt gacggtcgga
     4561 aaagcagcag acatcttgat gaatggttat gtcgcaaata cacctgatcc aaagctagat
     4621 gaactgcttt ctcgaggcgg gatcatgagt atgttaggtt ctgctagttt gattttactt
     4681 gcattggcat taggtgggtt actgatcaaa tatctgatcg ttgataccat cgttcaagaa
     4741 ttaaaagaaa agatggaccg accttctcgt ttagtcgggt ttactgcctt gagctgtatc
     4801 ggaatcaatc tgatcgttgg agaacagtat ctgtccatca ttcttccagg agaaacattc
     4861 aagcgttctt ttgatgacgt tggcttatca aaaacctatt tgacacgaac actagcggat
     4921 tcaggtgcga ccgtcaattc tctcgtaccg tggggagtca gcggcacatt cattatggga
     4981 acgatgaaag ttagcgcgtt gcattacttg ccattcgcct ttttctcgat tcttgcacct
     5041 gtctttacaa tattaggcgg ttttttattg aatcgtcgac gtgtaaaaaa ttgaaggaag
//
LOCUS       DACENJ010000170         5099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.176, whole genome shotgun sequence.
ACCESSION   DACENJ010000170 DACENJ010000000
VERSION     DACENJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.176"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1182..1847)
                     /locus_tag="IW748_002171"
     CDS             complement(1182..1847)
                     /locus_tag="IW748_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1105075.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1973..4009
                     /locus_tag="IW748_002172"
     CDS             1973..4009
                     /locus_tag="IW748_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1105076.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4013..4651
                     /locus_tag="IW748_002173"
     CDS             4013..4651
                     /locus_tag="IW748_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1105077.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4679..>5099
                     /locus_tag="IW748_002174"
     CDS             4679..>5099
                     /locus_tag="IW748_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105078.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTG"
BASE COUNT         1678 a          779 c          938 g         1704 t
ORIGIN      
        1 ctaatatatt catatgagct aattttatga ttaaatggta agtgtttcct tgtttgtttt
       61 agtttaaact agtgtgtaat ttaaaaatct atatggaagg gtctgtgggg taatacttaa
      121 ataaaggcta ggaacctgat aacatcaagg ttcctagcct ttttataata aaatgaagcg
      181 aaaataattt cttttaaaga gccgagatta tcagctatat aagagtgaat ttgatgcaaa
      241 aaacagagcc gaaacagtct tggtgttcgt agctctgctt atatgtttcg aatttaagac
      301 aaagagttgg caaaagatgt tgataaggaa gataagataa ctgtgttata taaactaggt
      361 aactaactgt aacaagctaa ttttaagaga ttcttattag aagacgttta ggaatataac
      421 taagaaaatg attatacagg taattgtcat ttcaataatg ttttctaatt ttcgcgtctt
      481 tgttttaaga aaaaggccag aatcagttgg aaaaaagttc gtacgatagt gaagaggggc
      541 aaaatttcct actaatattc ccactaacaa gagataaaac ggccaggttg gtgtgattcc
      601 tctataaaaa aatgcccatg ctaccgaaat cccatagatt aacaactgaa caatttttac
      661 aatgaaaaaa ttgttctgcc attactcaaa gtctcttcct ccttgtatcg tttatttgtt
      721 tcattttatc attatgatag gcgcttgaac aatccatttt gtattttatt gagtactctt
      781 ggataaacta ccagcgatta tcatatttat ttatgttact gtaagccatc atataaaagt
      841 aaatgagcag atcatagaaa tataaaaaag aactaaaatt gatctaaatt atgtgcagct
      901 tgactacgta ccaatctata ttacttacta gattggtcgt taatctattc ggatcgaatg
      961 agcaaaatat ttttgtgaat gtccatcctt gattatgatt ccaaaaagaa tcattgggag
     1021 gctgtggcgt aaatattata ctataagcca gaaataccta tcattctgaa cttttttatt
     1081 taggaacttt catctcaagg ctttttcaat taatttgtac ccctgttttt tggataagat
     1141 catgtgcatt gattttttct atctctacgt tcatctccta cttaatgcct atctggctcc
     1201 tgagggcttc tttttaaggc gtcgtcatag tcttggctga ggatttcttg gtaatctgag
     1261 ggaatggctt cccaagtgag caggcgaacg ttcatgacgt gttttccttt gtgtttgatt
     1321 acagcatggt ttttctcttt ttctacctct ccagcgtgaa aagacagcca tgtaccatgg
     1381 ccaaagatat cattacatac ccaacgttcg atggaattgt ccacgtatcc tacagggagt
     1441 gtggtatagc cgatttgtgg tttcaaaaaa ggattgaatc gatcgatcgt ttcatttttg
     1501 tattgataat tgataagaaa aagatagacc aaacaaaaga cgagcagaaa gaaagatagt
     1561 gtctttttga gtgagaaggg ttgttttgat gaataagatt tcttgttaac aagaggttca
     1621 tctggcaatg ttggttctat tgattcggta gctgattcaa tagaaggaaa atcaatcggc
     1681 agacctaaag tatgatgtaa aagtgacagc atttcaaggt ctggcattgt acgagaattt
     1741 tcccaactag aaatcgtttt atcggaaaca ccaatttttt cgcctaattc tttttgcgta
     1801 agcttacaat ttctgcggta agttcgtatt ttttctccgg tgttcatctt cttctccttt
     1861 cgattctaca gtatatagat ttattctaca tgacctatac acttgataga tacgtttttt
     1921 tgatgaaaaa cggtatgatt ttatcaaaga atcatttaag taagggagag tggtgataat
     1981 caaaaaaaag ttcacagttt tactcacaat catagtgacc ggtatcatga gtggttttgc
     2041 cttggttagt ataaataaac aggtactcta tcggacaaat gaatttttcc atcaaacaga
     2101 tacttatact ccgattcgat tgccacaaca ttttattggg caagaagaat atgagcagct
     2161 aatcatggta tttgaaaatg tctcatcaga aactgagctt tcttatatga aaaaaacagt
     2221 aaatgaatcc aatgaagtag atacatcttg gaacttcaag atacagccaa agcttgaaat
     2281 gacattgtat tatcaagggg atgtcaaatc ttctcacgag aaggtaccct attctaatac
     2341 ctcctttcgc gtaacgtcca ttgaagaatc gatcaaaata aaagattttg aaggtgaatt
     2401 tttcttgatg acgagagaaa aagaagccta tgaacggttc atttcactat tcactgatcg
     2461 attcaatcaa gcatttcaca ctgattatgc cactaatatt tttgaagact ttgaaaatag
     2521 ctattcgtat tcggttattt caaatacaga cgtatataat tatcgagtgt atttaactat
     2581 tggattgatt cttttattcc ttttgttatc atcctttttc ttattgatga ataaagaaat
     2641 cagtttattg ttgaataatg acttttcatt acatcaagta ttgtggtgtt tattggggaa
     2701 aaagaaaatc attattacat tcataattgc aggaatcatt ggtggtttta tttttatgta
     2761 ttataaggag caatttcttt atttgttcca gatgtacgtt ttcttgttca gcggaatata
     2821 tggtatctct ttgatttctc ttttattagt tgaaaaaatc gcttcgttaa gagaagcgtt
     2881 caactatatc caatacattc tttttacatt tggattgttg ttttctcttc ttttgaccgt
     2941 tgccaatgtc gaattagcaa ctatcgtttt ttcttcttca cagttagctg tttcccatga
     3001 accaccaaaa gaaatcgctg gtgaggacta tcatgtattt tatccagtaa ccatcgggaa
     3061 aaaccacgtg gattttattt attccaatcg ttttgcatca gcagcagatc aggaattata
     3121 tgaaactttg aataaaaacg gaagtatatt agtcaatgta tcagattact tgaatgaaga
     3181 aaatgaacag tttggcagag ggataaaaat aaatctgaat tatcttaaga aatttccttt
     3241 gattgatgtc tctggaaggt tgatccaaat aaccgaaaaa gaaacgcacc ctgttatatt
     3301 gatccctgaa cgatatagac atacagattt aaaaaatgac ctggcgcaaa taacagaata
     3361 ttatcaagaa atcagtgaaa aagaaccgaa atttctattt ataaaaaaca atcaacccat
     3421 ttatacattt attccttcta tcccctggat cgaacattat ccggttgttg aaatactgac
     3481 attgaaaaac agtatgtatt tggaaagaaa tattctcagc ggagaaatct atccaccgtt
     3541 aaagatcaag ataggctctt tgtcgaaaga aaggttattt gagctgattg aagaaagtca
     3601 gttacgtgat aacttgcagt tatcatttcc gtatactcag actgaagaga tagctgtcaa
     3661 agtgttaagc agatcatttc attacttgat acagatattt ttactcactt tcttttcttt
     3721 cttcttgttg agctatgtca tgttatgcat atattttgtc tacaattgtc ggaaaatcgc
     3781 tatctttcgt tcaagtggtt attcgctctt tgaaacgtat aaagatttct tcatgatgaa
     3841 cctcatcaaa tggggaacga caagtgtaat cttccttttt cttatagaaa gagagcctaa
     3901 atatcttttt aatatttttt tctttagctt cattgatttt attctttcag ttgtttttat
     3961 tttgtctaca gaaaagaaaa gtcagttatt gctgatgaat ggaggttaac aattggagcc
     4021 gttgatagtg ataaaaggtg cgaaaaaaat cattcgaaac aaaacgattc ttcatttgga
     4081 tcgtttcact gcggatgctg gggatcttgt gactgttgtt ggagaaagtg gagcaggtaa
     4141 aagtacgtta ttgaatatat taggattgaa tgatcagttc agttcaggac aattctattt
     4201 gttcgatcaa gagacgaaac aattaactgg gagagagaag cgcttgataa aaagaaatac
     4261 catatctttt ctttttcaag attttgggtt gattgaatat gaaacaatcg attttaattt
     4321 agatattgga ttgaagtttt cgaatttaaa tgtttcagat aagcgaacag caaagtatga
     4381 ggcattgaaa aaagtaaata taaacaagaa gctaagtacg cctatctaca cattatctgg
     4441 tggtgagaag caaagaatcg cctgtgcgag agtcttattg aagaatagca aaatcatttt
     4501 agccgatgaa ccgactgggt cattggataa aagaaataag gataaagtag ttgaaatctt
     4561 atcgcaacaa gcttcccaag gtacgttagt tatcgtagtc acgcatgatc catatctgac
     4621 caaaatagga aacaagaaga tcatgcttta aaaatcaaaa aggaaaggat gtcgttttat
     4681 gaaaaaagca gttattattt gtgcactagg gataggtttt gttggaggaa acgctatgtc
     4741 atctgaagtg gaagcaaatt cagttcacgg tgaaatctat tacaaatctt taaagccagg
     4801 agagattcat tttgatgcta aaggtaatat tattaagtca aatttaccag tagaccggga
     4861 aatttctaga acagcaatta atcatagctc tggaagatgg gtttattata gtgatacaac
     4921 ttgtacgaga aaaacaggtc attctaacca ctatagtcgt gtctataatc gtcacggtgc
     4981 aaaagcgaaa gttggaaact ctattcgatc agcaaatgca acaaaaggag tatgggccta
     5041 tgcaagtgcg acaggaaata gaactgacac attctcatgt tggtacaatc caaccggtt
//
LOCUS       DACENJ010000171         5020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.177, whole genome shotgun sequence.
ACCESSION   DACENJ010000171 DACENJ010000000
VERSION     DACENJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.177"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(46..1137)
                     /gene="phnW"
                     /locus_tag="IW748_002175"
     CDS             complement(46..1137)
                     /gene="phnW"
                     /locus_tag="IW748_002175"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1105079.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            complement(1354..1833)
                     /locus_tag="IW748_002176"
     CDS             complement(1354..1833)
                     /locus_tag="IW748_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1105080.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            2192..2974
                     /gene="rpsB"
                     /locus_tag="IW748_002177"
     CDS             2192..2974
                     /gene="rpsB"
                     /locus_tag="IW748_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1105081.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3073..3954
                     /locus_tag="IW748_002178"
     CDS             3073..3954
                     /locus_tag="IW748_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1105082.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            4090..4812
                     /locus_tag="IW748_002179"
     CDS             4090..4812
                     /locus_tag="IW748_002179"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1105083.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            4815..>5020
                     /locus_tag="IW748_002180"
     CDS             4815..>5020
                     /locus_tag="IW748_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1105084.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITP"
BASE COUNT         1694 a          934 c         1039 g         1353 t
ORIGIN      
        1 cagccaaagt ccacagttgt tcctgcccaa tcaaaaatta cagttttaat cattaacgtt
       61 ctccttatag tctttgataa ttgctgtcag ttccaaaata tcttccggat agatctcgcc
      121 aatatttccg atacgaaaac attcatgatc gctgattttc ccgggataga tcacaaatcc
      181 attttgcttt aaatactgat aaaaatgatt aaaatcaaat cgccagtcct tagggtaaag
      241 aaaagaggtg atgaatggcc cctgatattc ttgaatgaaa ggctggaacc ccatctctgt
      301 cattttttgt cgtagcacat gattattggc gagataacgt gaatatcgag tttcgattcc
      361 tccttctgcc tctagttcct tcagcgcttg ttgaaaagcc aaaaccgtat gggtagggga
      421 agtgaaacgc catttgccat tctcgttttc catcacgcgg tactggtcat atagatcaag
      481 tgacaaactt cggctaacat tttttcgttt ttccagttcc gctttattag caagtacaaa
      541 agaaaagcct ggaacacctt gaatacattt gtttgagctg gaaatcaaaa aatcaatccc
      601 tagttcggct acacaaatcg gtatcccacc aaagcttgac atcgcgtcca ctataaacaa
      661 aagatctctt tcttgtgtga cagcagcaat ctcttccaaa ttattcaaaa taccggaagt
      721 agtctcgcta tggaccattg ctagtgcgga gatagacgga ttctcttcaa tcagctgtgt
      781 aatcacatga gcttggagga tttgatgttc cgatacctca taaacggtat agttcagttt
      841 cagacattcg cacatgttga tcattcgttg tctatatgcg ccgtttgaac aaatcagtac
      901 agtatcttct ggcctgatca cagaacttaa taccgattct accccgaagg agctgctacc
      961 ttgcattaag accgttgtgt atattccttc cgatacttga gcaagatcca ataatttttt
     1021 tcttatccat tgtgtaattt ctttgtattc ttgatcccaa gtacaatgat cgatcagcat
     1081 agcatttttc actgtttcag aagtcgtcaa tggtccagaa gttaataatt tatacatatg
     1141 atttcctttc atttcttttt tgaaatacac aaagagcaag aagctgctct tgttaacatc
     1201 caatataaat gatgattatt gtgattcaat caaggtgttg aaaaatttga ataaaaaatg
     1261 atgtattttt gtaaagagtg taaaggttta ttcaataagg aaaatattga aaataaaaaa
     1321 acacgcattc catattagga atacgtgctc tgattaaaaa agtgatactt cagttggatg
     1381 gtgaagaaat tcttctactc gttttgcgtc ctcctcattt tccgagatga ccatgatcgt
     1441 atcgaatcca gcaattgtac tgacaataga gggaggtttg atctcatcta aaacagatgc
     1501 aaataaatga gcattgtctg gcaaggtatt gacgatcgtc aaaaagtgga ctcgttcgac
     1561 tttcaataca atgtcttcaa tcatttgaac cagccgatcc tcttcctcag tcctagtctc
     1621 aggagttcct ttttcgcgaa acaatacgaa gcgggaaacg ccattttcat caggtgcctt
     1681 cacgattttt agatcacgga tatctcgaga aatcgttgcc tgcgtggcgg taacgccata
     1741 atgcccaagt ttgtttaata gttcttcttg cgtacggatc gggaattctt caatgacttg
     1801 tttaatcagc aaatgtcttt gagctttgcg cattttcttt ctcctttacg aaagatttca
     1861 catgtatcat aacaaaaaaa agaaggattg cccaataata ttcgtctttt atacctaaaa
     1921 ttattttatg gaaaaaggat aataaagaaa caaagagaaa tctactgttt ttcttgaatt
     1981 ctttttatct ttcaggtaaa ctattgaatg ttgagcagaa aagtatattt gctcttgcga
     2041 tacggatctt ttgtctgtat aatagctgag ggtgctttat gcatcagcaa atccacattc
     2101 ttattggatt gtcggtaggt gcttgaaaaa gtttgccggc aaggtagaag ataagaagag
     2161 gaaaaataaa accaaatcgg aggaaacaaa catggcagta atttctatga aacaattact
     2221 agaagccggc gtacactttg gtcaccaaac tcgtcgctgg aatccaaaaa tgaagaaata
     2281 tatcttcaca gaaagaaacg gaatctacat cattgacttg caaaaaacag ttaaattagt
     2341 agatgcagct tatgattaca tgaagagcgt tgctgaagaa ggcggcgtag cattattcgt
     2401 aggtacaaaa aaacaagcac aagaagcaat caaagaagaa gcaactcgtg caggacaata
     2461 cttcgttaac catcgttggt taggcggaac attgacaaac tgggatacta tccaaaaacg
     2521 tatcagccgt ttgaaacaaa tcaatgcaat ggaagaagat ggaacttttg aagttctacc
     2581 taaaaaagaa gttgctggtt tgaacaaaga acgtgaacgt cttgaaaaat tcttaggcgg
     2641 tatcgctgat atgccaagaa tcccagatgt aatgtacatc gttgacccac gtaaagaacg
     2701 tattgctgtt caagaagcac acaaattaaa tatcccaatc gtagctatgg ttgatacaaa
     2761 ctgcgatcca gatgaaatcg acgtagtaat cccttcaaac gacgatgcaa ttcgtgccgt
     2821 taaattgatt acttcaaaaa tggctgatgc tttcatcgaa ggaaaccaag gtgaagatca
     2881 agtagttgaa gaagatttca cagaagaaaa cacagcaaca tcaatcgaag aaatcgtaga
     2941 tgttgttgaa ggcgacaatt cttcagcaga ataattttga atgatggagc tgtttcaaag
     3001 gctgggcgac aagcctcact ctcctttgaa gcagcttttt ttaaaaagaa aaacacacag
     3061 gaggagttaa acatggcaga cgttacagct aaaatggtaa aagaactacg cgaaatgact
     3121 ggcgtaggaa tgatggatgc gaaaaaagca ttagttgaag tagaaggcga catggaaaaa
     3181 gcagttgatc ttttacgtga aaaaggaatg gcaaaggctg cgaagaaaaa tgaccgcatt
     3241 gcagctgaag gattggcttc agttgctgtg aaaggtaaca ctgcagcaat cgttgaagta
     3301 aactcagaaa cagactttgt ttctaaaaac gaaatgttcc aagatttagt aaaagaaatt
     3361 gcagaacttg ttgctgaaaa caaaccagca gacatggaag ctgcaatgaa aatcaaaaca
     3421 gataaaggta caattgaatc tgacttgatc gaagcaactc aagttatcgg agaaaaaatc
     3481 agcttccgtc gctttgaagt tgtagaaaaa gacgacaatg ctgcatttgg tggatacttg
     3541 catatgggcg gacgtattgc cgtattgact gttctagatg gaacaactga cgaaacagtt
     3601 gcaagagacg tagctatgca cgtagcagct atcaatcctc gttatgtaaa cgaatcacaa
     3661 attccagaag ctgaattaga acatgaaaaa actgttctta cagaacaagc attgaacgaa
     3721 ggcaaaccag cgaatatcgt tgaaaaaatg gtagaaggac gcttgaagaa attcaaagca
     3781 gaaatcgctt tggtggacca accattcgtt aaagatcctg atatgactgt tgaaaaatac
     3841 gttgcatcaa aaggcgcaac tgttaaaaca ttcgtacgtt tcgaagttgg cgaaggtatc
     3901 gaaaaacgcg aagataactt tgtagaagaa gttatgagcc aagtgaaaaa ataattctgt
     3961 tgaataacag acgggaacgc acggtttgtg cgctcccttt ttttaagcaa aacaagaaaa
     4021 accagtctta gagtgtcgaa atcatctaag tatggtaaaa tggcaataga aaatattgga
     4081 ggaatgaaca tggtcacacc gaaatatcag cgtgtagtct taaaattgag tggagaagct
     4141 ttagctgggg acgcaggatt tggaatcaaa ccaccagtaa tcaaagaaat catccaagaa
     4201 atcaaagaag ttcacgagct gggcgttgaa atggcaatcg ttgtgggcgg cggaaatatc
     4261 tggcgcggac aaatcggagc acaaatgggc atggaacgtg ctcaagctga ttacatgggt
     4321 atgttagcta cagttatgaa tgccttagct ttacaggata cgttggaaaa cgtgggtgta
     4381 ccgacacgcg tgcaaacttc gattgaaatg cgtcaaatcg cagagccata tattcgccgt
     4441 aaagcagaac gtcatttaga aaaaggacgg atcgttatct ttgcaggcgg taccggcaac
     4501 ccgtacttct cgacagacac aacagcagca ttacgcgcgg cagaaatcgg cgcagatgtt
     4561 atcctgatgg ctaaaaacaa tgtagacggt gtttattctg ctgatccgaa agtagatgca
     4621 aatgcagtaa aatttgaaga actgactcat ctggaagtta ttgccaaagg attacaagtc
     4681 atggattcaa ctgcaagttc actaagcatg gataatgata ttccactgct tgtcttcaat
     4741 ttaaatgaac acggaaatat tcgccgtgca atcttaggcg aaaatatcgg aacaactgta
     4801 agggggaaat aaaaatggca gatgcaatta tgacagaagc aaaagaaaaa atgaaaaaag
     4861 cagcacaaaa tcttcaacgt gaacttgggc aaatccgcgc aggccgtgcg aacgctagcc
     4921 ttttggaccg tattactgtg aactactacg gtgcgccaac accattgaac caaatggcat
     4981 caatccaaat tccagaagca cgtgttttaa tgatcacacc
//
LOCUS       DACENJ010000172         4986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.178, whole genome shotgun sequence.
ACCESSION   DACENJ010000172 DACENJ010000000
VERSION     DACENJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.178"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..182)
                     /locus_tag="IW748_002181"
     CDS             complement(<1..182)
                     /locus_tag="IW748_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1105085.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="IW748_002182"
     CDS             517..951
                     /locus_tag="IW748_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1105086.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="IW748_002183"
     CDS             974..1294
                     /locus_tag="IW748_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105087.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="IW748_002184"
     CDS             1435..1626
                     /locus_tag="IW748_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105088.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="IW748_002185"
     CDS             complement(1697..1954)
                     /locus_tag="IW748_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1105089.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="IW748_002186"
     CDS             2112..2375
                     /locus_tag="IW748_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105090.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="IW748_002187"
     CDS             2399..2587
                     /locus_tag="IW748_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105091.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="IW748_002188"
     CDS             complement(2670..2855)
                     /locus_tag="IW748_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105092.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="IW748_002189"
     CDS             3070..3441
                     /locus_tag="IW748_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1105093.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="IW748_002190"
     tRNA            complement(3591..3663)
                     /locus_tag="IW748_002190"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="IW748_002191"
     CDS             complement(3709..4137)
                     /locus_tag="IW748_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105094.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="IW748_002192"
     CDS             complement(4197..4955)
                     /locus_tag="IW748_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1105095.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1737 a          782 c          977 g         1490 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atcttt
//
LOCUS       DACENJ010000173         4969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.179, whole genome shotgun sequence.
ACCESSION   DACENJ010000173 DACENJ010000000
VERSION     DACENJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.179"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1083)
                     /locus_tag="IW748_002193"
     CDS             complement(<1..1083)
                     /locus_tag="IW748_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1105096.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIE"
     gene            1272..2033
                     /locus_tag="IW748_002194"
     CDS             1272..2033
                     /locus_tag="IW748_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1105097.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2148..2588)
                     /gene="fabZ"
                     /locus_tag="IW748_002195"
     CDS             complement(2148..2588)
                     /gene="fabZ"
                     /locus_tag="IW748_002195"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1105098.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2808..3563
                     /gene="fabI"
                     /locus_tag="IW748_002196"
     CDS             2808..3563
                     /gene="fabI"
                     /locus_tag="IW748_002196"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1105099.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3673..4686)
                     /locus_tag="IW748_002197"
     CDS             complement(3673..4686)
                     /locus_tag="IW748_002197"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1105100.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4851..>4969
                     /locus_tag="IW748_002198"
     CDS             4851..>4969
                     /locus_tag="IW748_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1105101.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDA"
BASE COUNT         1514 a         1004 c          881 g         1570 t
ORIGIN      
        1 ttcaattaat ttaaagcttg accatggttt caaaaagtca aggaggaata attcgtcatc
       61 ggagattctt cctacaacat tgacacgtcc gtcgtttttc atatccttca aggcgatttg
      121 tgtttctcct ttgtattgac cgtacccttc attgtcaatc agtacatccc catgatgcat
      181 atctcgcgta ttatgaggcg ggaaatcttt atctttgtag tatacacgag tcatcgttga
      241 acgaaggata tattctgagc gatcccctcg ataactatga aggttatcaa acaagcagat
      301 tcgttcgttt tctgtaatac cttcttccgt atctactttc aatgttggat aatccgcgtt
      361 aaatgcttct gccatttctt tcaattcttc ttcacttgcg tacgcatttc caatagaaat
      421 atcatcaatc aatcctgtaa ggattaaatg cttcacttgt gtcgcaattt caagatctcg
      481 gtggtcttcc agcgtgcaca agccatcttg cgtaggccaa gggccaaact cagcagattg
      541 cgaattgacg aatgccatcg tattcaaatt atattttctg aatttttctg aacaaaaaac
      601 aaagtgttcg tatcctaaac cagagtatcg atgaggataa aaattatgag aacctaataa
      661 attatccgtg tttggcgaat aactcatgat gttgtcaacg taattcgtgc ccgaactcat
      721 attgatctcg attttgatac caaaaggatt gcgcgtcatt tttgcttctt ctgctcctgt
      781 gaatccgata tctaagcgga ccccgtaggc acccatctta tggaaaaaag ataagtcatc
      841 gtaagaaatc tccaactgtt caaataacgc tggattgata tcaaccatta cttccatgcc
      901 taggctattc gcgtaatcaa ctacttcttt aaattctgaa agaacttttt ctttatcatc
      961 attgatctgc agcaaactgg taaaaacccg tttataccca tacttatgag ctaaatccaa
     1021 gtacgctttg tccttttcaa atgttgaccg ttctggataa atcgagattc ctaattttcc
     1081 catattacca tttcctcctg catcttttcg tttttgtatc cacttacatt atagcaaaca
     1141 caaagtaaat ggtctacacc ttttcttttt cttttaaaat aaatgataaa ttccaataat
     1201 ttaagtaggt attttccctc tattttgtta tcattaaaga taacaaaata gtaaattgga
     1261 aaggaacagt tatgaagaaa aataaagtta ttttaagtat tagtttagca gcagcattgc
     1321 tgtttgctgc tggttgttct gcaaacaaca cgacttcttc tagttcgtct acagaaagtt
     1381 caaaaagtac atctactgcg gaaagcacga ctgccagtag cgtcgatttg aacgcattgg
     1441 aattaccaca attgacgaaa gatgtcgccg aaaatgaaga tctggtcgaa atggtgacga
     1501 ataaaggcac aatcaaaatc aaattatttc ccgaacacgc accaaaagcg gttgaaaact
     1561 ttatgacaca tgcaaaagac ggttactaca acggactgac tttccatcgc gtcatcaaag
     1621 atttcatgat ccaaggtggc gatcctaaag gcgatggcac tggaggggaa agcatttggg
     1681 gagaaggatt tgaaacagag atcaacaacc acctttataa tataagaggt gcgttatcta
     1741 tggctcgctc acaagatcct aacagtaacg gcagccagtt ctttatcgtt caaaatacag
     1801 atgatatgca tgatggtctt ttaaaagacg actatcctca agcaatcatc gacgcttata
     1861 aaaacggcgg ttatccttca ctagatggcc attatactgt cttcggccaa gtgattgaag
     1921 gaatggatgt cgtggatgct attgcaaata tcgaaacaga tagttcagac aaaccaaaag
     1981 aagaagtaaa aatcgataaa atcaatatac tgcaagaagc aaaacaaaaa taaaaaaata
     2041 acgtctgaat cctgcataga tgacaacgta tgctaggatt caggcgtctt ctttttacat
     2101 aaaaagcaag taagcttcct tttgttttgc tatatcaggt tttatagtca agcattttgg
     2161 ttgacgatga acgtgaactc gcactcacag acttttttat catctacata tgctgcagat
     2221 aacgctgtac cgactgatcc cttcattttg gtgatttcaa aatgacattt cagcacatct
     2281 cctggaacaa ctttttgacg gaatttggcc ttattgatcc caccgatata agctcttttt
     2341 cctagaaact cttctgattt taggataagt atcgaacctg cttgtgccag tgtttctaga
     2401 atcaacgcac ccggcatcac tggattgtca ggaaagtgac cttggaaaaa atcttcattg
     2461 attgttacat tttttgtcgc aacgatcttt tttcctggtt ctaaagtatc tacataatca
     2521 atataacaaa taggataacg attaggaatc aattccatta cttcagtagc agataatacg
     2581 taagacataa ttaaactcct tctttcaggc aattgccatt ttcattactt tgactatcaa
     2641 agtatcatat tcctaaatat ttagcaatct ttttttaatt taagaaaaaa tagtttgaca
     2701 atcaaaacat tttgactaat aattaagaaa tcaagaagtt attttagcta ttattctcta
     2761 tttttttacg actatcaaat ttttatcaga aacaaaggag agtcattatg tcatttctag
     2821 aaggaaaaaa aatcgtcatt atgggcgtcg ctaacaaaaa aagcattgct tggggttgtg
     2881 ctgaagcaat gttgaaacaa ggggcagaaa ttatctatac ttaccaaaat gaacgaatga
     2941 aaaaatcatt agtcaaattg gtcggcgaag aagcattcac tgtagaatgt gatgtttcta
     3001 gcgacgaaag catcgaaaaa gcaatgaatg ccatcggtga atatgctggg gagatccacg
     3061 gccttgtcca tgcagttgct tacgccaaca aagaagaatt gtccggaaat gtgtctgata
     3121 tttccagaga cggctatcaa ttagctcaag atatcagcag ctactcttta attgctgtat
     3181 caaaatacgc acaaccgttt cttgcaccaa acagcgggat cgttagcatg acttatctag
     3241 gttcagaacg cgcaatcccg aattataaca tgatgggtat tgcgaaagct tctcttgaag
     3301 ctgctatccg ttatttggca gctgagttct cgcctaaagg aattcgggtc aatggaatct
     3361 cagctggggc aatcaaaaca ttagctgtca caggtgttaa agactatcaa aaactgatcc
     3421 agttatctga atctcgtacg cctgatcagc aaggtgtgac gatcgaagaa gtcggtaatg
     3481 cttgtgcttt cttaatcagc ccactttcca gcggaatcat cggtgatatt atctacgttg
     3541 acaaaggtgt ccatctttca taaacttttg ttgcattctt taaagtaaaa catataaaaa
     3601 tcaggaaaaa actttttaaa gtctcttcct gattttttct ttttacgttt ttttcaactc
     3661 attactttga aattattcca cgacttcaat ccagccttct ggtgcttcta tgtctccaaa
     3721 ttgaatacct gtcaattcat catacaaccg ttttgttact ggtcctactt ccgtttcact
     3781 ataaaataca tgaagattgt tattgtgata aatcccaccg attggagaaa taacagccgc
     3841 tgtaccacat gcaccagctt ctgcaaattg atctagatca tcaatgaaaa tatctccttc
     3901 aattgcttgc agtcctagtc gttcttcagc taactgcaac aaggaatatt tcgtaatact
     3961 tggtaagata gagggcgatt gcggagtgat gaattttccg tcttttgtaa ttccaaagaa
     4021 atttgctgca cctacttctt cgattttcgt atgtgtggct ggatcaagat aaacacaatc
     4081 tgagtaatcc agttcttttg cttctgttcc tggaagtaaa ctcgctgcgt aatttccgcc
     4141 gacttttgct gcaccagtcc ccttaggagc agcacgatcg taatcagata caataaaatt
     4201 cgttggtttt agtccacctt tgaaatacgc tcctactggc gtacagaaga cgacaaataa
     4261 atattctgga gctggatgga caccaatgtt ttcaccgacc ccaatcagta aagggcgcaa
     4321 gtataatgta gcgcctgttc catagggcgg cacaaaatct tcatttgctt ttaccacttg
     4381 cttgacagca tcaacgaaca tcttttcagg gaccattggc atcagcaatc gtctagcact
     4441 ttgattcatc cgtcgactat tttgttcagg tcgaaagaga ttgaccttgc cgtcttttcg
     4501 acgataagct tttagacctt caaaacattg ctgaccataa tgaagcgctg gagagccttc
     4561 atgaatcgtc aggtaattat cttctgtcaa ttctccttct tcccattgtc cgtctttcca
     4621 tttggcaata aaacgccaag gagttttgat ataagaaaaa cctagattgt tccaatcgat
     4681 ttgcatatac ttaccacctt cgttattttc tccacatttt atcattatcc atattatttg
     4741 tcattttatt ttcagctttt ttccttcatt atagttattt ttgctatttc cttttttgaa
     4801 aacttcttgg tacaatacag aaagaatcaa attttagaaa agaggatttt atgttttcat
     4861 tattagcaac tgccggtacg acagactctt ctggcgagac attgacagat caagctgtca
     4921 gcaaagtaaa tgcgtttcag cggttttggg acgggattga ttgggacgc
//
LOCUS       DACENJ010000174         4888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.180, whole genome shotgun sequence.
ACCESSION   DACENJ010000174 DACENJ010000000
VERSION     DACENJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.180"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            377..1039
                     /locus_tag="IW748_002199"
     CDS             377..1039
                     /locus_tag="IW748_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1105102.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            1274..1414
                     /locus_tag="IW748_002200"
                     /pseudo
     CDS             1274..1414
                     /locus_tag="IW748_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            1440..1982
                     /locus_tag="IW748_002201"
     CDS             1440..1982
                     /locus_tag="IW748_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105103.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFATYESGVSAYDETGFDGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGYSLDDKTRLRLFDIEGKQIGLNKFCKNGKEFATKDF
                     IKGYRLLLENYGINLEESVK"
     gene            1983..2550
                     /locus_tag="IW748_002202"
                     /pseudo
     CDS             1983..2550
                     /locus_tag="IW748_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2562..2810
                     /locus_tag="IW748_002203"
     CDS             2562..2810
                     /locus_tag="IW748_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105104.1"
                     /translation="MEEMDFLDLENLVEFGYTWKGMIGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            2829..3068
                     /locus_tag="IW748_002204"
     CDS             2829..3068
                     /locus_tag="IW748_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105105.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAQQKGYPDKMLKQIGYYM
                     PEERIEKESCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            3085..3669
                     /locus_tag="IW748_002205"
     CDS             3085..3669
                     /locus_tag="IW748_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105106.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEEYMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEQELILERQRILFSEQDSKRLYGLYDLQLNGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            3771..4052
                     /locus_tag="IW748_002206"
     CDS             3771..4052
                     /locus_tag="IW748_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ1105107.1"
                     /translation="MKVKSPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            4042..4383
                     /locus_tag="IW748_002207"
     CDS             4042..4383
                     /locus_tag="IW748_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1105108.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVWSLKAISKSRENISFKKVGMLSVSDTKKL
                     NSFIERLKRKV"
BASE COUNT         1824 a          569 c         1063 g         1432 t
ORIGIN      
        1 ccattttttt cattatgaat gagataaagt aatttctact actgcgatac tggtgcacat
       61 aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt tagaataaac
      121 ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa ataatatgtt
      181 ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc gataaaggac
      241 agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc taaagggaag
      301 ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt cgataaagta
      361 ggaatggggg atctttttga atacaataga atcacagttt gataaggttg cagaagatta
      421 cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata tgtctccaaa
      481 gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga aattagcaag
      541 ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc ttgccaaatc
      601 taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac ttaattttaa
      661 tgagaagttt gattttattg taagccgaac aacctttcat catttggatg atatagccag
      721 cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta ttcttgataa
      781 tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa tccaagagtt
      841 tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata atcacaacac
      901 atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac aaaattatta
      961 cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt gggcaatggg
     1021 ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt gtgattgttt
     1081 tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc ctgtatctta
     1141 tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag taccttccac
     1201 ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg taatttactc
     1261 ttgataaaat gtttttccaa gtgaaaatga caaaagtttt actacgaatt tgaaaaacgc
     1321 aggaaaaaca attggtattg aattgttaga ccatatcatc gtcacaagca aaaacacaag
     1381 ctattactca tttcaagaaa atggttcttt ataaaaatta gagaaaagag gaaaacaaga
     1441 tgggcgaaag aacacaagcg ttaattacag tcaaatatgg taacagcaaa acgaaagcaa
     1501 gcgtgtcgat tcattatcag tgggggtatg gtcgatatat gctgatggac gtattgaatc
     1561 aaggaattta tttaatcaat aaagcatacg acttacgaga taaatcaaga gaagaattag
     1621 caaaaggaat ctttgataga agtacagggg ttatcaattt tgccacctat gaatcaggcg
     1681 tgagtgctta tgatgaaaca ggatttgatg ggcgctatga accttatcaa gaaacgattg
     1741 aagatgtgat gaagttttct gataacaatg atggctatgc tcatttagag atggtgttcg
     1801 atgaaaatgg atatagtcta gatgataaaa ccagattgcg attgtttgat attgagggta
     1861 aacagatagg actaaacaag ttttgtaaga atggaaaaga gtttgctaca aaagatttta
     1921 ttaagggata cagattgttg ctagaaaatt atggaataaa cctagaagag agcgtgaaat
     1981 aaatgtttca aaaaagaaaa gaccaattcc ctaatttaga agtgaaaagt caaaaagtga
     2041 ttgatggtga aatgattgaa ctattccaaa aaaaattatg gaacgataga aactaccgca
     2101 agttgcaaat actattttca aggaaagaga aacacgttta aaggctttgc cgattctgat
     2161 gaagaattgg caaagaaaat cactgattgg cttgttagga gtgaacatgt tgctagagaa
     2221 gataccgtta gaaaaacgac tgatggggca gtcttaacag gtactaaatg tgtttgttgt
     2281 aatcatgaat ttgtagaaga tgaaggttgt taccgaatca atgaagatgg cgattatctt
     2341 tgccagagtt gccatgataa agaattccca tctcaaagaa gttggtttga atatatgcta
     2401 caagattacg agatggaaac agaagatggt gaagaagtaa tttgtaccaa agaagaattg
     2461 agtaagcgta gcgatgatga gattgaagaa tggtgtgtgt ggttagcaga atcagagaat
     2521 tgtgttgcct actacacaac agcagaatag ggggaatcag catggaagaa atggattttc
     2581 tagatctaga gaatttggtc gagtttggtt atacatggaa aggaatgata ggtattagca
     2641 gaagtcttgc taatgccttt tatgaaagaa attatgcagt ctatgtcctt tatgatgaca
     2701 atacagaaag ccttgttgat gaagagtata aattggattt agagaatgtt ttatatggga
     2761 tagaaaaaga agatttagca aaatatattt tttcatggtt gggtcaataa acgaaagagg
     2821 gtagcaacat gacaaaattc aaaagtaaag gtatgcaatg gtgttcttat tattatgttg
     2881 ggaatgatga agagcttttg agaaaagccc aacaaaaagg ttatccagat aagatgttaa
     2941 aacagattgg ctattacatg ccagaagaga gaatagaaaa agaatcttgt gaggttcttt
     3001 ttgatagtga acagctatta gttttgttaa ctcaaaataa attgatttgt caatttgaaa
     3061 ataattaata tggggtgaag aaccatgaat gagaataaga tggtattaag tgaatacgca
     3121 agattgagaa ccattaattg gttgaatggt atgcaaagta gtggcgcttt tgatggattt
     3181 gatgaagagt atatggactt tgaaagttta tcgaatgagg atattagata ttattttatc
     3241 gaatggtatt ttacagaacc agatgaagag gtggaagatg aacaagaatt aattttagaa
     3301 cgacaaagaa ttttattcag tgaacaagat agtaagaggt tgtatggttt atatgacttg
     3361 caactgaatg gattaaaagc gcagtacgtt gctgatagtt acgatgatat ggtagaacag
     3421 ggggcagata gggttgtcac ttattcagaa gatgatgaag aggaatggga aaactattca
     3481 gatgatgaga tatgctttaa tgatgggtat atgcctattg aaatcaccga agaagaatat
     3541 gataaagtgt ccgatagcga tgagattggt atgtgtacgg ttgtaagtgg attggatata
     3601 gatgtccaaa gagtagaggt tgcataccag aagatgaaag aacgtataaa aagtaaagta
     3661 gaatcttgat ttgattgttt gcaaagtagc caattaagag tataatataa tcataaatgc
     3721 atttaatgga tataataatg agtatattat actcaaaaga ggtgaacgga atgaaggtca
     3781 aaagtccaaa agaaaaaagc agaattcaag tcggaataga caaggattta aaagaaaacg
     3841 cagaaatgat attggaagag ttagggttaa atccaacaac agcaatcaca atcctttata
     3901 agcaagtggt cgctagaggt gagtttccag tagagataaa gctaagcgaa gaggaaaagc
     3961 aaggaatcag attgcaacag ctaactatgg atatgccagt tgatgttcta gatacagatg
     4021 aaaagttaga agagtggttc aatgaagcct aatgagatag taacggtcta tgttgcattt
     4081 acagataaaa atggtggtaa aagaagacct attttggtgg ttagtgataa agaagataga
     4141 gtagaatttt ttggaatcac aagtcaatat gagaagaaat cagaccaaat aaagagagtg
     4201 tattttccta ttgcagaatg ggaaaaagca ggtcttaaaa agcaatcatg gattgacgta
     4261 tggtctttaa aggctatttc taaaagccga gagaacatat catttaaaaa ggttggtatg
     4321 ctatcagtta gtgatacgaa aaagctaaat agttttatcg agagattaaa aagaaaagtc
     4381 taacaaggct tttctttttt gtttatatgt atattaagat atacgtttat ataaacgtat
     4441 ttatgtatat aaaaatacac gtaaatatat acgtgattat aaacattaat aaaatattaa
     4501 gaattgttaa atagtgataa atagtaatat acgaattata agatatattt tatgttaaat
     4561 gtatgtaaat aaataaaatg actgacggat gttgaatagt gataaataac aataattcag
     4621 acggatacag cgagtgaagt agcttgctac gaacgaacag gcagaatgaa acggcagttt
     4681 cattagagca agtcgcttgc gacgtagcgt tactgctgat gtaactggca agttacatag
     4741 agagagtttg cttgcaaacg aacgttacct acttgaatag aaataaaaaa taacagatta
     4801 gtgaattttg aataggagcc acaactcaac caaccgcttg cggtgggtga gtcccacgga
     4861 ggaaaagacg aacgaacggt cgcgtagc
//
LOCUS       DACENJ010000175         4861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.181, whole genome shotgun sequence.
ACCESSION   DACENJ010000175 DACENJ010000000
VERSION     DACENJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.181"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            196..651
                     /gene="tnpA"
                     /locus_tag="IW748_002208"
     CDS             196..651
                     /gene="tnpA"
                     /locus_tag="IW748_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1105109.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            916..1014
                     /locus_tag="IW748_002209"
                     /pseudo
     CDS             916..1014
                     /locus_tag="IW748_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1237..1467)
                     /locus_tag="IW748_002210"
     CDS             complement(1237..1467)
                     /locus_tag="IW748_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1105110.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1697..2515)
                     /locus_tag="IW748_002211"
     CDS             complement(1697..2515)
                     /locus_tag="IW748_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1105111.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            2676..3365
                     /locus_tag="IW748_002212"
     CDS             2676..3365
                     /locus_tag="IW748_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1105112.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            3379..>4861
                     /locus_tag="IW748_002213"
     CDS             3379..>4861
                     /locus_tag="IW748_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1105113.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQ"
BASE COUNT         1633 a          730 c          890 g         1608 t
ORIGIN      
        1 gggtcggtac aaaacttaaa agttttagac ccttttattt tcttaatgac ttcagtcagt
       61 tttgtgcgta aagcgtgcga aacaagaaaa ccgcgcgttt ctacgcgtgg aagaagtatc
      121 tttagcgata aaaactcctt tcgatatact agtgagtggc taccaaccgc tcatcagatc
      181 gaaaggagtt ttttcatgtc aaatgacgat aaaagtttag catatactcg ttggaattgt
      241 aagtatcata ttatttttac accaaaatat caacgaaaag taatctatgg gcaattacga
      301 aaagatcttg gaaaaatact taggaagttg tgcgaaatga aagatgtgga aatcatagaa
      361 gcacatgcaa tgctagatca tattcacatg cttgttcgag taccaccaaa aatatgtata
      421 tccagtttta tggggtatct gaaaggtcgt agtgcggtac taatccacga gcagcacgca
      481 aatctcaaat ataaatacgg aaacaaatcc ttttggtcga agagatacta tgtgagtaca
      541 gtcggattga atcaaaagac aaatgaaaaa tacatccgtg aacaagaagc tgatgatcgc
      601 attcgatata ttataagtaa aagagagtac gtagaaccat ttaaaaaata aagagaagta
      661 agtgagtgaa gatgagcagt tggcggtctt ttaaaaatcc ccttttaagg ggcagcgagt
      721 gtcgacccct tctaggggga ttaaaaatac acccgtcctc acgggtgtat gggttactga
      781 tagacttgat tttaactggt gtcagaaact tatgtccaga aagggtgatg aaattttggc
      841 aatttacggc tattcaagaa ttagcacaag caaccaagat tacaaaacac aaattcaaac
      901 attagaaaat gctggcgcag aaataatctt cctagaaaat cactggatta tcgaacacct
      961 ttggaagtat ttttgagtta cgtaagtatt gatgatctgt ctaacttaat ttgacaatta
     1021 agagtatatt tacttcagat ggtaaggcaa caaaataaaa tacaataggc agatgaacta
     1081 ataaataaaa tctgtattaa atacagataa attaatataa aaatcaaaag gatcataaac
     1141 ttttaacaag tgatgaaatg ggtggaaatt gattcttata gacagtttgt ctcataacat
     1201 tgaagtattt tgcaatttta gcattgaaac acttttttaa tcttcaacgt tgttcttata
     1261 aacaagatga ttgggaatct tttgaattaa cactgtatag cttagaacac cctttataga
     1321 aaacattcgc ttagcaatct taataattcc ttgtgcccga tgtcgtttag attcagtagg
     1381 ttcctgctca tcgatcatgg aaaaaatttg aaggatcaga tccttaataa atttatttaa
     1441 gagaggatga tccattgctt catccatact cgataaacta gtgataatga gctacacgtt
     1501 tttctttcaa gttgatcaat agcttccacg ataaaaatat ctttagataa aatcaagaac
     1561 cgtaggaact acagggatat ttggtcaata agagccaccg ctattagtaa aaaataagct
     1621 atcaagtatg ctctcatccc aagaattgcc tacttcaatc gttaaaaacc gttaggttta
     1681 gctaagtggg ggtagttcac tcaatacttt tatcaataat agagcgcaaa actttttctc
     1741 ttaattcaac tgaatcaata ttattgtttt gttcataacc atgaactaat agatcacaaa
     1801 tataaagttg tgatattttt ccagctaaag atccaccatt aagaaattct tcaatggctg
     1861 tttgtaaaac taagtctgca gactttccta taggtgaatg aatatagtta gttatagcaa
     1921 taatttttgc gccatttttt ttagctattt tcaatgaatc ataagtatct ttcgttttcc
     1981 cagataaaga aaaaatgatt actaaatcat taactgttaa taaagaagcg acttgggctt
     2041 gaaaatgagg gtctagtaca gcttttgctt gaactcctac gcgaagaaac atattttcta
     2101 aatcaagact agtatttcca ctagatccca ctccaaatat atatatgttt tttgattgag
     2161 tgatcatttt aatggcttca ttcaattttt cttcgtctaa aagcctaata gtggaataga
     2221 gagcttctgt taaactatta gcaatttcat catagtactt tacactagtc ggatgttctt
     2281 ttttttgact aaagtcttcc tttgcaattt caatttttaa atcagaaaaa ccagaaaaac
     2341 caattttttg acaaaagcga ataatagtag catcgccaac ttttgtgttt ttcttaatat
     2401 cactcatcgt actataaata atagttttcc cagaatttaa aataaaatca gcgacttttt
     2461 tttcagactt cgttaaaaat ggataatttt ctttaattaa atagatataa ctcatacaaa
     2521 ctcctctaca atttcaactt ttctccatta tactccaaaa taataaaaaa aactcctaaa
     2581 tactccattt ttatctttac ttttatgaaa gcggatgcta tattgttttt ggagttgtta
     2641 tccaaaaata ataaaaaatt ggagtgttta ggataatgga taaaaagagg tttttagatc
     2701 ttatttcaaa aggtttgatt gtttcctgtc aagcattacc tggtgaacca ttttacaaag
     2761 aggaagaaag tttaatgcct tattttgctt tggctgctca aagggctgga gcagttggta
     2821 tacgtgcaaa ttctgttcga gatattattg aaattagaaa gtgtgtatca ttaccaatga
     2881 ttggaattat taaaagagat tatccaccag aatctccatt cattactgcc actatgagag
     2941 aagtagatga attagtagaa accggtgttg aggtaatcgc tcttgattgc actcttagaa
     3001 atcgacatga tggactatct ataaattctt ttattactaa aataaaagaa aaatatcctc
     3061 atcagttatt tatggcagat atttctacat ttgaagaagg caaaaatgca tataaagcag
     3121 gaatagattt tgtaggaact actttaagtg gatatactga ggaaagtaaa aaacaagatg
     3181 ggccagatat agatttgatt aaaaaattgg tagaagaagg gatttgtgta attgctgagg
     3241 gaaggattca tactccagaa caagcaaaag agataaaaaa aataggagtt gcaggtattg
     3301 ttgtgggtgg tgctattact cgaccacagg aaattgcatc aagatttgtt aaagcattaa
     3361 gttaggggga gaaataaaat gtttaaaaaa ttttcacaat taggacgcgc tttcatgtta
     3421 ccaattgcta tattaccagt agccggatta ctattgggac tgggaggagc gttaacaaat
     3481 gagagtgcta taaatgctta tccattttta gatcaaccgt ggttgcatac aattttaagt
     3541 attatgagtt atgctggaaa tgcagttttt gcaaacctag cactaatttt tgcaattggt
     3601 attgctgtag gacttgcaaa tggagataaa ggaacagctg gtttagctgg tggagtagct
     3661 tatctagtat atactgcaac tattagtggg tttcttgaac ttttttcagc aaaagatgca
     3721 acactagata caggtgtagt gggatcaatt gttgttggtg gtactgttgc atttttacac
     3781 aatcgttata gaaaaattga attaccacaa ttcttaggtt tttttggagg atcaagattt
     3841 attccgattg tttctagttt agctgcaatc attattggtt ctttctttta tttaatttgg
     3901 ccaccaattc aaaatggttt agtagttgct ggtgaacaaa ttgcccaaat gggtagttta
     3961 ggaacttttt tatatggttt tttattgcgt ttaacaggtg ctgttggact acaccacacc
     4021 atttaccctt tgttctggta tacatcttta ggtggtaccg aaactgtagc tggatcaaca
     4081 attgctggag cccaaaatat tttctttgcc caactagctg atccaaacca cactggtctg
     4141 tttacttatg gtactagatt ttttgcagga agatttgcaa caatgatgtt tggattacca
     4201 gcagcatgtt atgcaatgta tcgtgcaatt cctaagaaaa atcgtaaaaa gaatggtggg
     4261 ctttatttta gcggggccct tacatcattt ttaactggaa ttactgaacc agttgaatat
     4321 atgtttttat ttgttgctcc ttggctttat gtgattcatg cattcttaga tggattatct
     4381 ttttattttg ctgatatctt aaatattcgt gtagggaatt ccttttcagg tggtttgatc
     4441 gactttttat tattcggtgt acttcaaggg aatgacaaaa ctaattggtt aaaattaatt
     4501 ccttttggta ttgcatgggc atttatatat ttctttgttt ttacattctt tatcaaaaaa
     4561 tttaaggtag ctattcctgg aatggaagaa gaatcagaag ttcattctga aaaagtaata
     4621 aacaaaaata catcattaca tgaagagtct cttatcatta ttgaagctct tggtggaaaa
     4681 gaaaatattg agtctgttga agcttgtgca actcgtttgc gtgttgctgt tacgaatggg
     4741 gagattattc aaaaacaaac aataaaagac actggtgcaa ctgctgtctt cgaagtgaag
     4801 ggtggcatcc aagctgtttt tggagggaaa gctgacttat taagtcaaga aatcaatcaa
     4861 a
//
LOCUS       DACENJ010000176         4795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.182, whole genome shotgun sequence.
ACCESSION   DACENJ010000176 DACENJ010000000
VERSION     DACENJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.182"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(367..1158)
                     /gene="msrA"
                     /locus_tag="IW748_002214"
     CDS             complement(367..1158)
                     /gene="msrA"
                     /locus_tag="IW748_002214"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1105114.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1349..1984)
                     /locus_tag="IW748_002215"
     CDS             complement(1349..1984)
                     /locus_tag="IW748_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1105115.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYRLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2250..3302)
                     /locus_tag="IW748_002216"
     CDS             complement(2250..3302)
                     /locus_tag="IW748_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1105116.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3315..4253)
                     /locus_tag="IW748_002217"
     CDS             complement(3315..4253)
                     /locus_tag="IW748_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1105117.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1556 a         1004 c          732 g         1503 t
ORIGIN      
        1 tatagttatt gtttgcgctt ttatacggaa tttagttcat cattaatgtg gaattcgatt
       61 aggtgtctat taattatcaa gatgattctc tacaaaattc catagaaaaa tcaagattct
      121 acaattcgaa ttctctttca catggaaata aaggctagta aaaaaacagg agttgtgaca
      181 aaagacttgt catacctcct gtttccttgc gacttgaagc tgaatgcttt gcgccacaac
      241 cttttcctcg gtgttctcaa agcaactcct cgaaataatt cataactgtc caaaaatatg
      301 aaaagctatt tttgtcagtt gctcattatt ttgtcggagc tgaacgcttt gcgccacaac
      361 cttcctctat ctatgtcgct gaaaaaataa atgaagctga tgcgaatgct tgatcttttt
      421 atatctttta ggttgcttct gataaaattg ttgatgatga ttttcagctg gccaaaaagt
      481 ctttgcttgg cgaatctctg taacgatagg acggctaaat cggttagatt gaatcagttg
      541 ttctcttgat ttttcagcca attgcctttg cttttcatta acaacaaata gaattgaacg
      601 atattgtttc ccccgatctt gaaattgacc aaaggcatct gtagggtctg aaatttgcca
      661 ataaatctcc aacagctctt cataggttat ttgacgtgta tcaaatatga tctcaacagc
      721 ttcaacatgc ccagtatatc caccactgac ttgatcgtaa tttggttttt tggtctctcc
      781 acctgtataa ccagataaca ctgagattac acctggtttt gtttcaaaag gctcaaccat
      841 acaccagaaa cagccaccgg caaaaatcgc ccgttcttgg tataagggtg aagttatctt
      901 atgtttggca taatcataag cctttgcact cgctgactcc cctgtgattc ttaaataaaa
      961 atccatcgca tctggtgtca aattatctcg tagtgctagc ggacgcaagt tgacctctag
     1021 tttttctatc acctgcttta tcgacatttt tgctaggtct tcttttgcct cttcaaattg
     1081 tgttctttcc cattctcttg tatttcgatt taaaataata ttataaaggt ctatttccat
     1141 ctgctccaca tctaacatga agtcaccctc tttcaagttt tcttcttatt cattcaaaaa
     1201 atgactacct gaaatttagc acaacgcata acaagttaag actaacttgc ttttcgctct
     1261 cctcatctct attcttagtc gaacacatcc acacgaatgg ttacccatag agaaattctt
     1321 ttacttaatc agcttcacac tgctttttct actttcgtag ttgaaaaaaa cttaaacaaa
     1381 tcatcggcaa atagttaaag aaaaaactta agcgttgaaa gaagcccaat tgcttaaaga
     1441 aggcaaactg actaatcgac ggaatccgtg ctaatctgta aagacctgca attagtaaac
     1501 taataactgt taaaacaaga taaaaatggc taagcgtacc ttgaccactt tgacgataaa
     1561 gcagaacaag taacaatggg aaaagtagaa agcctgcata gcctagccca gaacctatat
     1621 tatggatcca agtcgataga ttccaagagc tttcattcgt attgatactg aataacccag
     1681 taaaaataca atcacctatc ccatataaac caactgttgc aacagcaagt attgcaagtg
     1741 tttttgatgt tccaataaat aggtgataca acgcaggcaa cgaaagaaca aaaaaaagtc
     1801 cagatacaac tgaccagacc aagaaagcac gtctgactgg actatctacg tcaccaaaca
     1861 cactaattac ggtagtcatt tgattcagtt tgggataaaa aaggccaagg atatacggtg
     1921 tcgataaatc actaatcaca cctaataata aaaaataaaa accatatttt tgcaaaaaaa
     1981 tcattttctt ctcctacttt ttagtgatta ttcgatacat ccaacaatca aattttttct
     2041 cattttaaca catattttta ttctagttgc cttttgaaca atcaataaca taaaggtgag
     2101 tttcttctac attttaaaaa atcgaatggc actgaagagt tcagttgtca gcatcaatct
     2161 cctactcaaa aaggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc
     2221 attgattgaa aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga
     2281 tcatacagtt taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg
     2341 atgactaacc aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac
     2401 ggaccaacaa gtccgaccac gccgaaacca gcagaagcag gcgtacctga aatagaaaat
     2461 agagcgacag gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt
     2521 tttctaaata gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca
     2581 cctgacttat taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc
     2641 atcgcagccg caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt
     2701 gaaataggag agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc
     2761 ggttgtaagg tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca
     2821 aaagggaata aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac
     2881 acaatcgaca ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat
     2941 gctgtcagca tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca
     3001 cccaaatcag gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc
     3061 atcattctca tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt
     3121 gttgctagtt gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta
     3181 aaatatttta aaatggcacc taatactgca ttaggaatca atccaataat gattcctgtt
     3241 gctgtaccag cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc
     3301 attcaatctc tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc
     3361 aagaaatcac aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca
     3421 ttgatataat cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga
     3481 taatgtaacc caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg
     3541 acatctagtt caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc
     3601 accattttat gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg
     3661 catagtccgt tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg
     3721 tcagaaaaat gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct
     3781 gcttcttttc catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc
     3841 tgaccaggac cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt
     3901 ggtacaaatt tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct
     3961 gtatcttttt tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg
     4021 gtaaatagaa taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt
     4081 ggtagttttg cttctacctc tttaccatta aaatttgctt gtaatccatg ctctttgatt
     4141 tgttggacat gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc
     4201 attaacccaa agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct
     4261 tcctcctttt tctttctatt atattcattt gagaatcatt tcacaacctt gttttgttat
     4321 tcttctctta tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata
     4381 aaaactggaa tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata
     4441 gaaacattca tagaaaaaac ctcactcctc tgattataaa agagggggca ttaacctcgt
     4501 gtatactaaa accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat
     4561 tgtagtacga tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa
     4621 caatacactt atttaaaaac ttctttccca gattttcttc catttttaca gttataacta
     4681 gtattatcat gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact
     4741 tttttattaa ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctg
//
LOCUS       DACENJ010000177         4759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.183, whole genome shotgun sequence.
ACCESSION   DACENJ010000177 DACENJ010000000
VERSION     DACENJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.183"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            140..886
                     /locus_tag="IW748_002218"
     CDS             140..886
                     /locus_tag="IW748_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1105118.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            883..1722
                     /locus_tag="IW748_002219"
     CDS             883..1722
                     /locus_tag="IW748_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1105119.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            1723..2673
                     /locus_tag="IW748_002220"
     CDS             1723..2673
                     /locus_tag="IW748_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1105120.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            2814..3566
                     /locus_tag="IW748_002221"
     CDS             2814..3566
                     /locus_tag="IW748_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105121.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKNADQQIQFVQLKKEKLYYGLYSR"
     gene            3585..4736
                     /gene="queG"
                     /locus_tag="IW748_002222"
     CDS             3585..4736
                     /gene="queG"
                     /locus_tag="IW748_002222"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1105122.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         1625 a          790 c         1023 g         1321 t
ORIGIN      
        1 tgattctaca gaaaattttc ataaaatgcg aggaaaattc gaaaaaaact ttttcatcat
       61 tctacttgaa aatgctttcc attttagtta aactatattt aaataaaaag ttaggtaaac
      121 ctaaaaagga gtgtaagatt tggaacgtac ggccattgta attcaagacc tttcagccgc
      181 ttatcaaggg aaaactgttt tgaaaactat caacttgacg ataaatccac aaaaaattac
      241 cggtattatc ggaccaaacg gtgcaggaaa atcaacattt atgaaatctt tgctagagtt
      301 gataccagct actactggta aagtcacctt ttctggacag tcggtaaact cagtccgtaa
      361 aaaaatagcc tacgtggaac agcgaagtga attggatctt tcctttccaa ttgatgtatt
      421 aggcgttgta cttttaggaa catatccatc tttacgaatt ggacaaagac ctgggaaaaa
      481 agaaaaagaa cgtgcaagac aagctttgaa aaaagtaggg atggaagaat atgccaaaag
      541 acagatcagc gaactatcgg gtggacagct tcagagagtt tttattgcaa gagctctagc
      601 ccaaggagca gaatggatct ttttagatga accattcgta gggattgatg cgttaagtga
      661 acgaaagatc tttgacatct tgcaggaatt gaagaattca ggaaaaacga ttttgatcgt
      721 ccatcatgat cttcataaag tagaagaata tttcgatgag gttattcttt taaataaaca
      781 gctgatcgct tccggtccag tacaagaagt ctttacatcg aagaaccttc aattggccta
      841 tggtgagatc atcagacatt tagtaaaagg aggagagaaa aaatgatcca atcctttatt
      901 gatggattaa tgaattatca attcctgcaa tacgctttgg tcacttccat gcttgtagga
      961 cttgcttctg gagtcatcgg ttcttttatt attttacgag ggatgtcttt gatgggagac
     1021 gctatctctc acgcagttct tccaggtgta gccatttctt atatgtttgg ttttagttac
     1081 atctttggag ccactgcttt tgggatgcta acagcagcag tgattggttt cgtgactcag
     1141 cggagccgat tgaaaaatga tacagcgatt ggaattgtct ttagctcctt ttttgcatta
     1201 gggattatct tgatttcata tgcccagagt gcgacagatt tgtatcatat tttatttggg
     1261 aatgtcttag ctgttaggga aagcgatttg ttgctgacag cactcgttag tggaatcgtc
     1321 ttgatatttg tctttttctt ttataaagaa ttgaagatca catcattcga tccgacaatg
     1381 gcaaaagctt atgggctgaa cacatcgctg atccattatc ttttgatgtt ctttttgaca
     1441 ttagtggctg tagtcagttt acagacagta ggaacaatct tggtgattgc catgttgatc
     1501 acaccagccg ccacggctta cttgctaacg aaccatttac tgaaaatgat cattacagct
     1561 gcaggaatcg gtatgctaag tgcagttgtc ggtgtgtttt tcagttatag ttacaattgg
     1621 ccatcaggag ctacgatcgt gttagcatgt accgcatttt ttatccttgc ttttttattt
     1681 tctccaacaa aaggaatttt atttcagaaa ggaagcagat agatgaaaac aaaaaaatca
     1741 ctctttttaa tcttggcagt tagtttcttg gtattagccg gttgcggaaa gcaggcttct
     1801 gaccaagcgg atgaaggaag taaagaaaag ttgtcagtcg tggctaccaa ttcgatcttg
     1861 gcggacatgg caaaagaggt cggaacagat caaatagata tccacagtat cgtaccagtc
     1921 ggaacagatc cgcatgaata tgaagtacta ccagaagaca tcaaaaaggc aagcgatgca
     1981 gatgttattt tatacaacgg tttgaatctt gaaacaggta acagctggtt cgataacttg
     2041 atggaaacgg ctaaaaaaga agaaggaaaa gattattttg cagttagcaa aaatgtagaa
     2101 cctctttatt taactagcgg tgaagaacat acaaaagcag atccccatgc ttggctagac
     2161 ctatctaacg gaataaaata tgtggaggaa atcgcacgta tattctctga aaaagatgca
     2221 gaaaatgcga cactctataa aaaaaatgca gaagcatatg tggaaaaact aaaagaatta
     2281 gatacacaag cgaaggaaag ttttgcttct atcgaagaga acaaaaaatt attagtaaca
     2341 agtgaagggg cattcaagta tttttcacga gcatatgatt tgccagcagc ttatatatgg
     2401 gagatcaata cagaaagtca aggtacgcct gatcaaatga aagcgatcat tgatcagata
     2461 agagcaaaag aagtaccggt tttattcgtg gaaaccagtg tagactcaag aagcatggaa
     2521 cgggtagcaa aagaaacggg tttgaaaatc tatgataaac tgttcactga ttccatagca
     2581 aaagaaggag aacaaggaga ttcttactat cagatgatga aatggaatat tgaaacgatc
     2641 catgaaggtt tgtcacaaac aaaagaaagc tgaaaagtgc aaggtctagc ttgaggagca
     2701 gaccttgcat tttttataag agatatgaaa aaaaattatg ctgtttttcg tcgtacagat
     2761 accttttttg aagaaattcc gttatagtta gagtaaggaa ggaaggtgtg gctatgaatt
     2821 ttacacgtaa agataaatat acgattaaag aagaagtctt tcgattgatc agccagcagg
     2881 aaaataccga caaggatcaa gaccttcaat ttcttgaagc agaattagct aaattattca
     2941 tagcaaatca aaatgagaca acagaaccag atgaagagct gtacataggc aaatggttgt
     3001 ttgatgaatt tcagccttta gatgaaaata ccggtatcgg ttattttgtg ttcctttctc
     3061 cagtcggctt cacacctggc gtattagtta aacaaaacga cgaaataacc gtcaaagcat
     3121 ggcctgcatt attttcagtc atcaaagcag aaggagtaat caaaaagatc cagtcggcct
     3181 attataaaag tcaatttatt gccaacagca aattaatgaa tatgttttat aatcgtctag
     3241 tagaaattgc taggcctggg aaaatcccgc aaacattcac gtacacacag caaaaaaatc
     3301 tctatgaaga agtattgtat tcccgcgatc tgtctgagtt ttcgccatgg gaagaggtaa
     3361 ggctccatga actgttaagt acaaaaccag tttcattgaa tttttttaat gcattagata
     3421 acaaaagaaa attaatttca acaaattatt gggaagtatt tgctgcaaga aaaaaatgga
     3481 tggaagaaaa gaagaatgcc gatcagcaga tccagtttgt ccagctgaaa aaagaaaaac
     3541 tatattatgg gttatacagc cgttaacagc aagcgaggaa gaccatgaca ttaaaagaag
     3601 aaatcataca agaaagcaaa cgattaggaa ttgacaaaat cgggttcacc acggcggaac
     3661 cttttgattc attaaaagaa tcgttggaag aacaaaaagc agctggccat acttctggct
     3721 ttgaacatcc aaatatcgat gagcgtcttt atccagaaat gacatttgat cagccgaaat
     3781 cgatcatttc cattgcactt gcgtatccta ctaggatcca tgaagaagtg cctagggatg
     3841 aaaaacgcgg gcagtttgct cgtgcttctt gggggatcga ttaccatgat atactcagag
     3901 accgtttgaa ccgattgatc gaattcatcc aaacaagagc agaaaaatgg caaaaagaag
     3961 aggaatggcg tctagctccg caagtagata ctggagaact gatcgatgtc gctgtagcac
     4021 agcgagctgg tttaggattt attggacgaa acggattgtt gatcactgaa gagttcggtt
     4081 catttgtcta tcttggtgag atcgttacta atattgtatt tgagcctgat aaaccaggag
     4141 aatttggttg cggtgactgt atgcgctgcg tgactgcttg tccgacaaaa gcattgcttg
     4201 gtgacggaag aatgaatgcc cagcgatgtt tgtcttatca aactcagaca aaagggatga
     4261 tgccggaaga atatcgtaaa aaaatgcgaa atgtcattta tggatgtgat atctgccagt
     4321 tagtctgccc ttacaatcaa ggaaaagatt ttcattttca tgaagagatg gaaccaaaag
     4381 tagacgaaat atatccaaaa ttaaagccaa tgctttcgat gtctaataaa gacttcaaac
     4441 agcaatttgg gcatttagct ggttcatggc gtgggaagaa acctttgcag agaaacgcat
     4501 tgattgcttt agctaatcta ggtgatcgaa cggcattgcc tgagatttca gaatgtttaa
     4561 cagatgtccg ccctgtcatc agaggaacag cagcttgggc tattggacgt ctaggaacaa
     4621 aagaaccgga aaaatggctt gatgtattag atgaaatatt agctaaagaa acagaagaag
     4681 tcgttataaa cgaattggaa catgcccaga aattattgcg gagaaagtta aaataaaaaa
     4741 atagttaaca ttattcaaa
//
LOCUS       DACENJ010000178         4699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.184, whole genome shotgun sequence.
ACCESSION   DACENJ010000178 DACENJ010000000
VERSION     DACENJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.184"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(6..707)
                     /locus_tag="IW748_002223"
     CDS             complement(6..707)
                     /locus_tag="IW748_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1105123.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(815..2224)
                     /locus_tag="IW748_002224"
     CDS             complement(815..2224)
                     /locus_tag="IW748_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1105124.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(2272..4137)
                     /locus_tag="IW748_002225"
     CDS             complement(2272..4137)
                     /locus_tag="IW748_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1105125.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(4258..>4699)
                     /locus_tag="IW748_002226"
     CDS             complement(4258..>4699)
                     /locus_tag="IW748_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1105126.1"
                     /translation="LFVKQKTGVELSEDEAGFIAFHFVNSQQGNEDMQVTMTATTMVR
                     DILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLKGEGSDEQDEFLYALVQGKYPK
                     AFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVSEKKSIQS"
BASE COUNT         1346 a         1077 c          802 g         1474 t
ORIGIN      
        1 tttatttatg tttcataatc aatatggtaa ggatgcagct ccgtatggta gaccgcctcc
       61 gaatattcaa tcatttcccc aatttgcgta gacgatttcc gtatccgttt gatgccgatc
      121 tgtgcgtctg ttttcagaat tccttgttct tcttctgtta aaaatactgc cgagaaatca
      181 tcagaaaatg agtcaatagt atatcctttt ttcgtcagca aacgatacaa agattctttg
      241 ccaaattctt ctctgctgaa ttgcatcatt tcttgtggaa gataatgaac aaaataaata
      301 taaggctgat tgttcaaata atataagcgc ttaaaacaaa cagcttgctc accgaaaaag
      361 gaaaacaaag gatcttcctt ctttatatgg accaattgca aatccagcgt ttctttccgg
      421 acttcatatc gggagttctc caagatttgc gtaaagcttg ccgctttgga taatcgattg
      481 taaggacgat tgcttagaac cgtcgtccca cgaccacttt tctttgcgac atattcatct
      541 gatgccagca gttcgattgc ctttcggaca gtgattttgc tgacttgaaa cagttcttct
      601 aattcacttt ctgtcggcaa catcgaaccg actggatatt ttcctgagaa aatatcttct
      661 ttaattttat ctgcgacttc caagtatagt acacgtgtcc ttttcataac gtcacccttt
      721 gctttactat tactaaaaga atagcacata aaaaataaaa aaagaacaca aatcacatgt
      781 gagaagtgtg ttctttttca ttcagctgct ttaattacaa gtttacgcca tttgaggcaa
      841 ttacttcttt ataccaatca aaagattttt tctttgatcg attcagtgtc ccatttcctt
      901 catcgtctag atccacatag ataaagccgt aacgtttact catttctcct gttgatgcac
      961 tgacaagatc gatacatccc caaggcgtat agcccatcaa atctacaccg tctgctactg
     1021 cattagccat cgcttcgata tgacgacgta aataatcgat ccgataatca tcaataatcg
     1081 tgccatcctc ttccatatga tcaatggctc caagaccgtt ttcaacaatg aataatggtt
     1141 tctggtaacg attgtataat tcattcaatg cgattctcag cccttctgga tcgatctgcc
     1201 agccccattc acttgcctca agaaacggat tttttactcc tcccagcaag ttcccttctg
     1261 aagttgctgc ttgtggatca gtctcattga ttgccgtaga catataataa ctaaatccga
     1321 tgtagtccac tggatattct tttagcaact ggaagtcttt ttccgtttgc tctaattttt
     1381 ccggatcgac tccatatttt ttatgggtac gcgctgtgta tgcagggtat tctccacgta
     1441 cttggacatc cgtgcagaaa aaattgaatt cttggttttg gatcattgtt gctgcctgat
     1501 tgactggatt ggcatcgatg ctgtaagttg ttgcatagat aatcatgcag ccgatttgga
     1561 tatccgaacg caattcgtgg ccgattttta cggctttgct acttgcaacg aattgattat
     1621 gccatgcttg gaaaatattt tggtactctc ctgctccatt gctttttact aatccttgac
     1681 ttaaagctgg aaagtgaaag gccgaattga tttcattgaa agtcatccaa tatttgactt
     1741 tactgctgta tctctctaat actgtctgag caaatcgttc atagaagtcg atcagttttc
     1801 gatttttcca gccaccgtat tcttttgcca aatgaagagg catttcgtaa tgagaaatcg
     1861 tgatcacagg ttcaatatca tattttaagc actcatcaat cagctgatca taaaattcta
     1921 atcccgcttc gttaggagta ccttcgtctc catttggaaa aattcgtgtc caggcaatcg
     1981 aaaaacgata acatttgaat cccatttccg caaacaaggc aagatcttct ttgaaacgat
     2041 cgtaatgatc gattcctcga tgattcgggt aaatatattt ttcttgatca atcgtccagt
     2101 caaattcttc actgccgata atcgcaaagc gctgcttacc tccaggcata gcatctgcaa
     2161 cggatagtcc ttttccgtct cgatcataag ccccttctaa ttgatttggc agcagttgct
     2221 ccaccccata aaaatttttc tgaaaaacgt gattgcatcc tgatttcctc cttattttac
     2281 aattgccaga acatcttcgt ttgcaataat ctctttttgg tctaattcaa gtatatcggc
     2341 aaatgcatca gtattcgtaa tgacaactgg agtgattacg ctatagccag ctgctctaat
     2401 tgcttcgatg tcaaaacgaa tcaataaatc tcctgctttg acgagatccc cttgtttgac
     2461 taagatctca aagccattcc catttaattc tactgtatcc atcccgatat gaatcaaaat
     2521 ctccgctccg tcagtcgttg tgatccctac agcatgtcca gttggaaaaa tagtcgtgat
     2581 ctctccgtct gcaggtgcaa ctagttctcc tacttctgga tcaatagcga taccttttcc
     2641 catcacgcca gaagaaaaaa cttggtctgg tacttcactc aatggcagta ctttacccgt
     2701 taaaggactt tttaaggtat gccgtgctga agtaatcggt tctcctgcat tcgcatgttt
     2761 gttttctaat tgattgtctt gtgtttgttc gtcaaatcct aaaatcaatg tacctacgaa
     2821 tgcaagaaca aaagcaattc ctgtagctat tactgctact gtcacatttg actctacacc
     2881 atccaccgta ttaatgaaag tcggaatcgt caataagctg accaaactgc tggagaaagc
     2941 ttttactccg gaaaatccga taatagctcc accaatacct ccagaaatac aagcggcaat
     3001 gaatggtttc ttcaaaggta atgtcacacc atacacagtt ggttcagtga ttccaaataa
     3061 cgaagtcact gtagaagaaa tccctaatgc tcgaagtttc gtatcttttg ttcgcaatgc
     3121 tacagctaaa gcagccccac cttgtgccaa gatggctgga agaagcattg gcatcaatac
     3181 atcaaaccca tattgttcga tattcaaaaa catgatcggt acgaatcccc aatgcatgcc
     3241 gaacatcacg aatacttgcc ataaacttcc catgattaga cctgctacga ttggactaaa
     3301 tccataaatc gagttgaaga gaccgcctaa agcattaccg gcaaccgtcc cgattggtcc
     3361 taaagcaaga aatgttaatg gaaccatcac tactaaaacg acaagcggaa ctaaaatcat
     3421 ttgcagaaat tgcggaatca ctttttttac gaatggctct aatttacttt gaacccatac
     3481 ggctagaatg attggtatga cagaagaagt ataaccagat ggtgaaagaa tcactgggat
     3541 cccaaagaaa ctgatagctt ctcccgcacc agcaactgct gtgatagctg gatacacaag
     3601 tgccatagca attgctactg ctaaaaactg gttggctttg aactttttag ctgctgtaaa
     3661 agcaagcatg acaggtaaga aggtaaagac accatctgct gcagcaaaca atatttgata
     3721 agtaccgcta tcagctgtca gccagcccat caccgttgcc aatgataaga atcctttcaa
     3781 taccccggcg gctgccatcg cccctagaaa aggtgtaaat atactagaga taatatcgat
     3841 cagacgattc aaaatatttt tggactcttc ttttccatcc tccgaaactg caagaccttc
     3901 ctcatccacg atcgattgat aaacatctgc tacattactg ccaatcacta cttgatactg
     3961 accgccactt tgtacgactt ggatcacgtc cgggtcttct tttaactttt gcgtatctgc
     4021 taaagattca tcttttaatc tgaatcgcaa ccgcgtagca caatgtacta aacttttgac
     4081 attcttttgt cctcctaccg cttcccatac acgacgacca actgcttgat tatccattgt
     4141 tttttccttc tttctttttt atttaaaaaa taaaaaccca gattgaaaaa aacagtcaca
     4201 aacattcgtg aacctatttt ttcaatccag gttttgcctg ccatacagta acaatcctta
     4261 tgattggatg ctttttttct cacttactac tctttgaata tgaatcgtta aataaatctg
     4321 ctctgcgatc cccatttcct gtttttgcgt ttgtcgaaga tactcattga tccgttcgac
     4381 acaatgaaac gcttttggat actttccttg aactaatgcg tagagaaatt catcttgctc
     4441 gtcagatcct tcccctttga gataacgctg ggcaaaaaac tgcagatgag taatgatacg
     4501 ctgataattc aaagattctt catcgaacac gatgccgaag aatttactga tgatactcag
     4561 tatatctctt accattgtgg tagcggtcat cgtcacttgc atgtcttcgt ttccttgttg
     4621 gctattcaca aaatgaaagg caataaaacc ggcttcatcc tcagaaagtt ccacacccgt
     4681 cttttgtttc acgaacaaa
//
LOCUS       DACENJ010000179         4685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.185, whole genome shotgun sequence.
ACCESSION   DACENJ010000179 DACENJ010000000
VERSION     DACENJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.185"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            394..1527
                     /locus_tag="IW748_002227"
     CDS             394..1527
                     /locus_tag="IW748_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1105127.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1558..1878
                     /locus_tag="IW748_002228"
     CDS             1558..1878
                     /locus_tag="IW748_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105128.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(1907..2371)
                     /locus_tag="IW748_002229"
     CDS             complement(1907..2371)
                     /locus_tag="IW748_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1105129.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2503..3757)
                     /locus_tag="IW748_002230"
                     /pseudo
     CDS             complement(2503..3757)
                     /locus_tag="IW748_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(3786..4103)
                     /locus_tag="IW748_002231"
     CDS             complement(3786..4103)
                     /locus_tag="IW748_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1105130.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(4129..4449)
                     /locus_tag="IW748_002232"
     CDS             complement(4129..4449)
                     /locus_tag="IW748_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1105131.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         1489 a          944 c          841 g         1411 t
ORIGIN      
        1 tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt
       61 tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac ctctttttat
      121 ttatgtctgt ttctttttga aaacaactat tttttcaacg tttactctct tgccaatcca
      181 acatcatcta acggatacaa ctatttcaat gattaccatt acactaaaaa atcggaaaca
      241 cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa aaaaagcgtt
      301 tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac taataatgca
      361 gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa
      421 cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac agatgcaatc
      481 atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca tcatggtgaa
      541 gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg
      601 ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt taatattagt
      661 tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga atcaaatcca
      721 gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca ttggggtggt
      781 caagcggaat atctaagagt tccttatgca gactctactt catttgttgt accagataat
      841 gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc ttattggagc
      901 gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc tggtccagtc
      961 ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat cgcagtagat
     1021 ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac ttatttctta
     1081 gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc
     1141 attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc gaaaaattta
     1201 gtaagcttac aaagcggcac aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa
     1261 tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta cccgctgcaa
     1321 gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat ccatctaatg
     1381 ccaaaaattt acgcaatgat tgcagaagga ctattcgatc ctacacaaat cattactcat
     1441 tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa agaagataaa
     1501 aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat tttctttatg
     1561 cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga tatcgaaata
     1621 cgaggacgtc gcttccgctt ctatttccaa aatcgttaca cattgatctc actggctgtc
     1681 gatttattaa ccgggatttt ctatattttt ggaagccttg cttccttaac taaaatcccc
     1741 gatcgatatg gcacctattt ttatttggct ggtgctattt tcttgacgat tcgtcctatt
     1801 ctcagaatcg taaggaacat ttttatttac gacgaaaaaa aagcaatgaa gagaacaagc
     1861 aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct cttttttcac cctatcgtat
     1921 cactttgata cagacgagcg gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc
     1981 actcgtatcg ttcataaaac gtggtatgat ctgtaattaa gtaaagtttt tcgtacccaa
     2041 gttgatgcat ttcttttctg atttccgcta atatccgctt ggctatcccc tgcttacgct
     2101 tttccggttc aacaaacagg gcacaaagat ttggtgtcag atcagggcgg ttatggaagt
     2161 cgttttcaat gacgcccgct cctccgacga tggtttcttt ttcatctaag accacatacc
     2221 attgaggaat actttcctta ttagcgattc cagtacgaat actttcctca tacacaacag
     2281 caggaactcc ccattttgat gaaaaccaat tcgcgctttg ttttactaat tctgggtgtt
     2341 ctcttaattt gatccagtta tatgtaacca atgtttaaac ctccgactaa aataaaacgt
     2401 ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga caacaatagt gatgtaaaaa
     2461 aaaaaacgga aaacaatcac ttgttctccg ttttttgatt gatcaagcat ctaaagcttc
     2521 ttttcttctt tcttggatcg tagctacctt gacgaacggt agatagatca aaatagaaag
     2581 aaccaaattg acagcggcta gtacaccacc tgcaatactt tttgttgcca atacaccgcc
     2641 aataattggt ggagttaccc atggaggcat aacggtagct gctggcacta aacctgtaga
     2701 agtcgcaaag taagcaacac taactaagac cattggtgtc aaaataaatg gaatgaacat
     2761 gatcgggttc aacacgatag gtaacccaaa catcgttggt tcattaatat tgaaggacac
     2821 ctggcgcaat cgacaagttt gttacaacca tatatggttt gtttttacgt cctactaaat
     2881 aaatagctaa cagtaaacct aaagttgcac ctgttcctcc taagttgaca aagctgtcaa
     2941 agaaaggttt gttcacgata aacggtgcgc tatgcccagc tgcgattgca ttaacgtttg
     3001 cttcaatggc tggcgcattg attgtttgca tcaaaggatc gaccatattc gctccgtgta
     3061 aaccaaagaa ccataagaat ggtgtgataa atgcaagcag taaggcagat ggataagagt
     3121 ttgcaagtcc cataaatggt tcttggacca ctgtatagaa agaacctacg atatcatcaa
     3181 caccgaatgc agcaaaaatt gcagcaacta aagcaaacgc agagatagtg atcattgctg
     3241 gtagtaatga agcaaatgct tttgcaactg ccggtggcac accatcaggc attttgatga
     3301 tcaatttttc atttccgctt aaacgtacaa agatttctgt tgaaataatc ccaacaataa
     3361 tagcaataaa caaaccgttt gagctcatac ctaatgctcc gcctaaagcg aagaaagagc
     3421 ctaaagaaat cacacctgcg gaaacaccgt cttttcggta gccgttagct aaatgataag
     3481 caagcaagaa agcaatcaag acagaaagaa tggcaaatgt accgttccaa gcagcagccc
     3541 cgaatccttt ccatgattca ccaccaaaaa ttgagttcat caattcttgg aacccgggaa
     3601 tcggtaagtt attgatcatt actgctaatg ctcctaagat cattaacggc atactcacca
     3661 taaatgagtc ccgaatcgca actaaatgcc gttgtgcacc aatttttgat gctacaggta
     3721 taaaatgttt ctccataaat gcaacaaatt tatccataaa tcttccctct tttctttact
     3781 gtagattacg cgttcaatcg acgatacaga tcaatgatct cttttgctaa atctgtaaaa
     3841 gctatagatg tcatcagatg atcttgactg tggaccgtca aaagcgtgac ttcaacgtga
     3901 tttccttgtg cttcttgtgt cagcatctct gtttgagagt gatgtgcttg gactaaagat
     3961 tcctctgcat ctttgatttt ttcatctgct aaagtgaaat cacctttttt cgcagcagca
     4021 atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc catgatggct
     4081 tctagatttt tttgatcttc catttattgt atcctccgat aatttttatt atttgtttat
     4141 tagtttttct gcttggtcga gtactttttc accattcatc atgccatagt cagccatatt
     4201 gattacatct aaaggaatgc ctttaggtgc tagcttttca gcaaattgat tttgcataaa
     4261 gcgtacttgc ggtcctaaaa gtaaaacgtc tacattttta gattctagtt gattatccgc
     4321 atctgaagca gatacagcaa agatgtctgc ttccattcct tttgcttctg ctgccttttg
     4381 catttttgtg actaggagac ttgtactcat tccagctgaa catacaagca tgattgtttt
     4441 cttttccatt ttaaacacgt tcctttctat acttacactt gtatataagc aagtagcgtg
     4501 ccaactttaa aagcgcttta aaaagttgat atcatcacat ttaaggtcta taccaagttg
     4561 catacacact aaaaaagaaa gcgcacacta aacacacaaa ttaattttta ttaccttttt
     4621 tcttttaacg tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt tttcaaataa
     4681 acggt
//
LOCUS       DACENJ010000180         4681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.186, whole genome shotgun sequence.
ACCESSION   DACENJ010000180 DACENJ010000000
VERSION     DACENJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.186"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(104..1336)
                     /locus_tag="IW748_002233"
     CDS             complement(104..1336)
                     /locus_tag="IW748_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1105132.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="IW748_002234"
     CDS             1591..2160
                     /locus_tag="IW748_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1105133.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="IW748_002235"
     CDS             complement(2338..2778)
                     /locus_tag="IW748_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1105134.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="IW748_002236"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="IW748_002236"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1105135.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="IW748_002237"
     CDS             3732..4655
                     /locus_tag="IW748_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1105136.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1418 a          866 c          926 g         1471 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat atattaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 a
//
LOCUS       DACENJ010000181         4677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.187, whole genome shotgun sequence.
ACCESSION   DACENJ010000181 DACENJ010000000
VERSION     DACENJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.187"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1858)
                     /locus_tag="IW748_002238"
     CDS             complement(<1..1858)
                     /locus_tag="IW748_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1105137.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRK"
     gene            complement(2056..2289)
                     /locus_tag="IW748_002239"
     CDS             complement(2056..2289)
                     /locus_tag="IW748_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAQ1105138.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            complement(2436..3290)
                     /locus_tag="IW748_002240"
     CDS             complement(2436..3290)
                     /locus_tag="IW748_002240"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1105139.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            complement(3275..3613)
                     /locus_tag="IW748_002241"
     CDS             complement(3275..3613)
                     /locus_tag="IW748_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105140.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            complement(3841..4458)
                     /locus_tag="IW748_002242"
     CDS             complement(3841..4458)
                     /locus_tag="IW748_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1105141.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
BASE COUNT         1333 a         1106 c          809 g         1429 t
ORIGIN      
        1 ctttccgacc tttcaactta tacttcatta atctcatgcc attgaaaatt accaccaaga
       61 tactaccttc atgaaccaac attccgatag acatattcat ccattcgctg aagaagacgc
      121 tggtcaacaa gaccaacact actccaactg aaatcacgat attttgtttc atattatttg
      181 cggtggcttt tactaatcct aatgcgtgag gcaagttgct gaagttcgag ttcattaaaa
      241 cgacatcgga tgtttcaatc gctacgtccg ttccgcttcc cattgcgatt ccaatgtctg
      301 ctaaagctaa ggacggacta tcgttaacgc catctccaac aaaggctaca atttgaccac
      361 gttgttgaag ttttccgata taagccgatt tgtcttccgg caacatgtag ccgtgagctt
      421 cggtcaaacc aagttcgccg gcgaccacat caacggttcc ttggttatct cctgaaagaa
      481 cgactagatt tttcacgccc aagtctttta attcctgcaa attagctttc acacccggac
      541 ggacttgatc gcgaatgccc atcagtactt ttaattcacc gtcgaccgct gtcaaaacga
      601 gagagttccc ttgttgttca aaccgtttga catctttttg cacttttttg ctgagagtga
      661 cattttcttt ttccatcaag gccacattcc caacagccac tctatgtcca gctacacttg
      721 aaacgattcc gccgcctttc acaacttcag ttccttcaac aggataaaag tttgtttctc
      781 caatttgatt taagaccgct tttgctaatg gatgatctga ttcccgttcc acactggcca
      841 gatagccaag cgtttcagca ggatcttttt catacagttc agtcgctgca acggttgggt
      901 ttccgacagt taaagtacct gtcttatcaa atacaattgt atccacatta ctgaagtctt
      961 gaatgacttc actccctttt aaaagaacac cgttacgagc accattccca ataccggcaa
     1021 cgtttgagac aggcactccg ataactaatg cgcctgggca acctagaacc aagatggtga
     1081 ttgctaactc aatattttga ctgaacgccc atacaacaat tgccagaacc aagacagctg
     1141 gtgtgtagta tttagaaaag cgatcaatga accgttccgc ttcggattta gaatcttgtg
     1201 cttcttccac gagttcaata atttttccaa atgtggtgtc ctcaccaact cggtcagctc
     1261 tgatttgaat cgttccgttt tctaaaatgg ttcctgcaaa aacttctgca tcagctagct
     1321 tgttgaccgg aatagcttca cctgtaatgc tagcttcatt gatatgccct tcacccgtaa
     1381 ggactatgcc atctactgga actttcgcac ccgttttaac gagcaagata tccccttcat
     1441 ctatatcatc cacttctact tcttcaaatt cgccattatc catttgtttc aaggcgcttt
     1501 cgggtgccat ttcagtcaat tctttgatag cagatcgcgt ttggttcaat gttcgttgtt
     1561 ccaagtagtg tccgaataag aataagaacg tgacaatagc cgattcttca taattttgaa
     1621 taaaaagcgc accaatcgct gcaatactga ctaagacatc aatactgatg actttgactt
     1681 tcattgcttg aaacgcttga atggcaattg gcgtaatccc aaagacagag gcgatgatca
     1741 aagaccactc tgaaagtcct acgttttcta aaacaaagtg actgaagaaa ccgagtacga
     1801 ttaacaatcc actgatgacg gtgataacat ttttcttgct taatatatat tgttgcatgc
     1861 tttttccctc caaatttaat ttctctttta ttgataaaca cagtataaga taattgccta
     1921 agtaattaat tgactgacat caagtttaga aagttctctt ttatcgtttc taagcacctc
     1981 gaatcggatt gcaacaaaaa aagcaatcct ggaaaaatag cttttcttcc agaattgcct
     2041 agttcattta atctgttaaa cggccttgac ttttgcttta agaaccggat agcctacgtt
     2101 ttcgatagct ttttgaatct cttcaatcga agtgacagct gaatcaaaat tggttttgac
     2161 tttgctggag ttgaataata ctttaatgct gtctttatca ataccgttaa ctgatttcac
     2221 ggcaccttca attttttgca tacaacttgg acaagacaac gtttctaatt gcaatatagc
     2281 tttttccata attcctatct cctttatgtg tttttatttt atcggcagca gattaatgaa
     2341 cactgccttt tcctctttac aactgtagta tagctcactt ttagcaaacg aaaattgacg
     2401 catatcaagt tttgcacttc tttttatctt tttgctcaat tcaccatctt aatagtcttt
     2461 ttctgcccct tattgccatc agaataataa gaaattggtg gataatcttg tctgcgtata
     2521 aaccatacta caatcgcgcc aataaacagg gccagagaca aggcttgcga aacgcggatc
     2581 cactcgccaa tccataaact gtccgttcgc ataccttcaa tgaagaaccg acccactgag
     2641 taccacaaaa cgtaactcaa agcgacttct ccttgacgca aaagctgttt tcggttccgt
     2701 atagtgacga tgaggacaaa gcccaacaaa ctccacaatg actcgtataa aaaagtcgga
     2761 tggtagtagg taccattcat attcatttgt tcaatgataa attcaggtaa ataaagattt
     2821 tccaagaatt gacgagtaac cggcccacca tgagcttcct gattcataaa attgccccaa
     2881 cgaccaatgg actgggctaa caatacattg ggcgctaaaa tatcaagaac gagtgccaaa
     2941 gaagtacctt ttttctttgc ataccaatat agcgtacccc ctcctgcaat caatccccca
     3001 taaatcgcaa tgccgccatt ccaaatcgca aggatttgtc ctggattttg cagataataa
     3061 ccaagttcaa ataacacata ataaagccga gcgccgagga gtccaatcgg aattgcccat
     3121 aaggccatat cgataatggt atcctcttcc agtccttttt tctttgcttc cttggtgctc
     3181 aactggagag ccgctaatat gcccgctgca ataatgactc cataccaatg aatcacaaca
     3241 gaaccaatct ccaccacatg gggattcaaa acgcctaata cgaatcccat tctatttgct
     3301 ccatttcttc aatttttcgc gtcattctag tcatggtgat gctgaacaag attccggcta
     3361 agctccatgg aaaccaaaca gtgaatacac ttttatctcc ctggtcactc gcttcgtaat
     3421 acgccacttt tccgtgtccg attacgccgg ctccttttac aacttcctct tctttttcca
     3481 tagctaacct atctgcctca acaacgttag acgtttttgc ttcgccgcca atcgtgttct
     3541 tggtgaaagg gacgacaaag aatgcccccg ctgctgacaa cgtgatggca actatcaccc
     3601 aaaaacaatg cacattcacc ctacttattt ttacgttttg ttcgacaacc ctatcttaaa
     3661 gaaaaataaa acaaaaaaac ttgagggtta tcatgtttgt gcttttattt ttccaatgaa
     3721 tgaaggtcgt tctgtcttaa aaaagccaaa aacggacaga tggaagcaaa agcactgagt
     3781 tctgtcccgt tgttaatgaa gagcgacaga acaccaacgt gtagaaaaaa gtgtgtccga
     3841 ttattcgctg taaagcaata aatcatctaa atcctgaatc ttgatccttt tcccagacag
     3901 ctgttgaatc aatccttcgt cctccaattc tccaaatttg cgactgatgg tctcaggtgt
     3961 cgttcctaaa taggaagcaa tgtccttttt tgccatcggc agggtgatgg tgggagagtt
     4021 gcccatttca ggttccacat tttctgccaa aaacaaaact aaacgggtaa tgacactctc
     4081 cacggctact tgtgtcgttt gacgttctga actttctaac cgtttagaca tttccgccaa
     4141 tagttttaaa gaaatagctg ggtattgttc cagaaaatct tggacatctt tttgctgaat
     4201 catacagaca acggtatctc gggttgcttc ggcatattcc tcgtgtactg catccgggtt
     4261 gaacaaggtc cattctccgg tgaaatcacc agggttcaag atccgcacta gctgctcttt
     4321 gccagagtct gccaatcgat aaatacggat tttcccacga ttgataatat ataaagtatc
     4381 ctctttttct tgtgaacgaa acagaaactc tcctttttga tactgtttcg tgtgggcttt
     4441 ttcagcaatg taattcatcg cgctatcctc taagtgattg aatattggaa ccaagcggat
     4501 acaggctgcg tggtcgccaa tttcatgatg ggtatgttta gtcaattcat tctccccctt
     4561 tatttatttt gtttatattc gctgctttta ttatataagt ctcttactac aaaaaaattg
     4621 atgcacatca agaaaatgga aaaaggactg gaaagaattc caatccctaa atcagct
//
LOCUS       DACENJ010000182         4628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.188, whole genome shotgun sequence.
ACCESSION   DACENJ010000182 DACENJ010000000
VERSION     DACENJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.188"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..769)
                     /gene="xth"
                     /locus_tag="IW748_002243"
     CDS             complement(17..769)
                     /gene="xth"
                     /locus_tag="IW748_002243"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1105142.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            882..1802
                     /locus_tag="IW748_002244"
     CDS             882..1802
                     /locus_tag="IW748_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1105143.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            1875..2810
                     /gene="murB"
                     /locus_tag="IW748_002245"
     CDS             1875..2810
                     /gene="murB"
                     /locus_tag="IW748_002245"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1105144.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3108..4586
                     /locus_tag="IW748_002246"
     CDS             3108..4586
                     /locus_tag="IW748_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1105145.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1628 a          740 c          901 g         1359 t
ORIGIN      
        1 ctttttcaca acctctttat ttcaaatcta attctactgg acaatgatca ctgcccataa
       61 tgtccgtatg gatctttgca tcagctagtc ctggctctaa atctttgctc gtcaaaaagt
      121 aatcgatccg ccaaccagca ttgtttttcc ttgaattgaa tcggtagttc caccaagagt
      181 agacaccttc ctgggttgga taaaaataac ggaatgtatc gataaacccg ttatccaaca
      241 gtcgagaaag agccgctctt tcttctggag tgaatccagc atttttttga ttcgttttcc
      301 agtttttcaa atcaatcttt tgatgagcga catttagatc cccacacacg atgactggtt
      361 tttgtttttt tagattttcc aaataattat aaaaggcttc ttcccattct aatcggtaat
      421 ctaaacgttt taattcgctt tgagaatttg gcgtgtaaca ggttacaaga aagaaatcgg
      481 aatattccaa agtaatcaga cggccttctg tatcatggat atcgataccc attccgtaag
      541 tggcattcaa tgctggtttt ttagcaaaaa tcgctgtgcc ggaatagcct tttcgttccg
      601 cgtaattcca atattgataa taacctggaa gatctaaatc aatttgtcct gcttggagtt
      661 tcgtttcctg cagacagaaa aaatcggcgt ctaactcatg aaaaacttct aaaaaatttt
      721 tatttacgat ggcacgaagc ccgtttacgt tccaagagat aaatttcaat aaaagcacac
      781 tccttcgaat taatttcctt acctttgcga tactattgta cctcatttta gtaaaaaatg
      841 gtgtaagatg aaagtaacta aatgatccag gaggaaagta gatgaagatt ggtgtaatcg
      901 gtgtagggaa tatcgcagaa aaagcatatt taccaactta tgcgaaaaaa caaggaacac
      961 ttgattttta ttttgcaaca agaaataaag aaacaaaaga aagaattcaa aaagagtacg
     1021 ggttccaaca tctatatgaa acgatcgatg agcttttaga agaaaaaatc gaagcatgta
     1081 tgatccacgc agcaacaaaa gtacattatg aattagccaa aaaatgtcta gaaaatggta
     1141 tccatgttta tattgacaaa ccactgagtg tccatctaaa tgaagttcgt gaattgcaag
     1201 aattagctga taagaaccaa ataattttga tgatcggttt caaccggcgc tttgctccta
     1261 tggtagaaga attgaaacgt atcccagata agcggttgat ccacctgcaa aaaaatcgaa
     1321 tagcggcaaa agaaacgaca gagttcgtcc tttatgattt gtttttacat cttattgata
     1381 cagctgtgta tttattagat gaacccattt tgagaacaaa ataccagata agagaaacaa
     1441 aggaattttt ggaatatgcc attttacaat tagaaacaca aaatcaaacg gctattttaa
     1501 cgatggattt agcaagtggc gctaatatag aaaaatatca agtaacaagt aaaaatggta
     1561 cttatgaagt aaacgatctg acccagcttg tcattcaaga tgctgatgga aaaaaagtga
     1621 aagaatttgg cgactggaca aacacgctag tcaaacgcgg gtttgagcca atggtagagg
     1681 ccttcttttc tcagatccgt ttaggtaaat cggaaacaga attgcttaaa caaaaagaag
     1741 ttgtaagaag tcatgaatta tgtgaggaag tgcttaaaga tcactaccgt catcaacttt
     1801 gaacaagact ggcaagattg tattaagcgt gatagaataa tgaggacgtt tgagaaaaga
     1861 aaggaaaatc ctcagtgaat aaagaagaaa tagttgaaag cttagccacg attactatat
     1921 tgtttgatga accattgatg aactatacgt ttacgaaaac aggtggacca gctgatgtcc
     1981 ttgcattccc gaaaaaacaa gaagaagtaa agcaaatcat cgattattgc cgaatccatg
     2041 atataccttg gatggtatta ggaaatgcaa gcaatctgat cgttcaagat ggcgggattc
     2101 gcggtgtagt catcatgctg actgaaatga aacagattca tgtcaaagga acgatggtca
     2161 ttgcggaagc aggtgcctca ctgattgata cgacttatgc agctttggca gaatcattga
     2221 ctggatttga atttgcttgc ggcatcccgg gaagtgtcgg tggagcggtt tatatgaatg
     2281 caggtgcgta tggcggagag atcaaagatg tttttgctga agtggatctt ttgcttgaag
     2341 atggaacatt gaaaactttg acaaaagacg aaatggtatt ttcttatcgt cacagtaaag
     2401 tccaagaatt acgtgctatc gtattggaag ctcgattcag tttgcaaaca ggggattatg
     2461 aagcaatcaa agcaagaatg gatgagttga ccgaattgcg tcaatcaaaa cagccattag
     2521 aatacccttc atgcggaagt gtctttaagc gcccagtagg acactataca ggtcagctga
     2581 tccaacaagc aggattgcaa ggcctgaaat ggggcggtgc acaagtctct gaaaaacatg
     2641 ctggattcat cgtcaatatc gatcatgcga cagcgacaga ttatattgaa ttgattgcgc
     2701 acatccaaga agtaatcaaa gaaaaatttg atgtgtcttt agaaacagaa gtgcggatca
     2761 ttggagaaaa acaagcaata tcagaaacaa gcagaagttt agccaattaa taagtaatgg
     2821 aacttaaaat aagtaaaaga gcaggacggt ggccgtcctg ctcttttttg tggttgttta
     2881 cgcaaatggc gcaaatcttg tacattttaa cacaataaaa caaaaaatat agaatatttc
     2941 ctcataaatt tttccatttt ttgcaatatt ctcaaaaaaa agataaaaaa tgagaataac
     3001 tgatgaaaaa acaagcataa tccatcataa aatcagtata ggtgaaaaga tgaactttca
     3061 tctgcatatc aagtaattgg tatgaggcat tttttgaggt gtaggatatg aagttggatt
     3121 accttataga agaggaagat agagaaaaac tgctgctagc tagatattta gaatttgaga
     3181 aaaagaatta tgtagtgctg cctaagttat atgaaacatt aggtttgtct aaatttaaaa
     3241 caagaaatta tatcatagaa ttaaataaag aatttactga attcaaaaca aagccagaga
     3301 tccaaatttc agaagaagca gagatccatg tcgaacgtct gaatttgacg gatatcaaaa
     3361 atttaagaag aatctacttt caacgatcaa aaattgctcg actgttaacg gagatcgtgg
     3421 attcaaaagt aacgatcgaa aaatttgcag agaatcattt tttaagcata tcaagagcct
     3481 atgtaaaaag aagagaatta gtctcttttc ttgaacagca tcaaataaag ttaaagaaaa
     3541 acatattagt tggggaagaa ataaacatac ggaacgtatt atttggggta tatttcgggg
     3601 tctttaatgg tttttctatg ccatttagag aaagcgtcat gaatgaagca aagagtatcg
     3661 tgcaatattt tgaatatctg ttcaatttaa aactgtctgc aacaaaacga atcaaattca
     3721 atttgctaac cactattagt ttgttgaggg cctatgggaa tcaaccaatc ggaaaacgtt
     3781 tcttttcaga tgaatacttt aagagctcag aaggaatgaa gatcatcaag gatttatcac
     3841 atatttcttt tcttcatatc cctaaagagc agatcattga tgagatttct tatatcatgt
     3901 tgtttttgat cacggaagaa gaatttgaat tagatggcgc atttggactt gcggaagctc
     3961 attttcatga tatccacatg attagtcaaa aaatgtcagt ggaattgatt gatcaattac
     4021 cattttcgat tgaaatctct gaagaagaaa gactatatat acgaaaagaa cttcgtatgg
     4081 gtatgatgca tgtcaatcga aaaaattgga tattcgactt tgagatgact agctttacgt
     4141 ctaaaaagca gctacagttt ttcttcgaga cgtatccaat attcagtaca gcaatccaaa
     4201 aggtcataca agagaataaa cgctatttct tgggaaaaga tgaacgtttc atgattcgct
     4261 tattttatga ttatctcttt ttattggtcg gtatttgtcc ggtatcatat tttgaagcac
     4321 cgattcatgt ctgtattgat ttttcgcaag gatctgctta taccgattac attatttctc
     4381 aagtgaaagg atttaaaaat tacaatattg tgattgagag tcgaatcact tctcgtacga
     4441 atatttattt atctgattgt ttacttgaaa aatttaataa agaacagata atttggaaaa
     4501 atccaccgac tccagatgac tgggaatatt ttggaaatac catcatcaga gaaaaaaata
     4561 gacatttaac tgaaaaaatc agctagtatt tatggatggc agctttgtct cacatattta
     4621 ttagctga
//
LOCUS       DACENJ010000183         4612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.189, whole genome shotgun sequence.
ACCESSION   DACENJ010000183 DACENJ010000000
VERSION     DACENJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.189"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            237..2081
                     /locus_tag="IW748_002247"
     CDS             237..2081
                     /locus_tag="IW748_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1105146.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2137..3603
                     /locus_tag="IW748_002248"
     CDS             2137..3603
                     /locus_tag="IW748_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1105147.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3640..4482
                     /locus_tag="IW748_002249"
     CDS             3640..4482
                     /locus_tag="IW748_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1105148.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1486 a          750 c          992 g         1384 t
ORIGIN      
        1 ttaaaaaatc attttattat ttacgtttac attctaatat gttaaattga atataactta
       61 aacaggcatg tgactggtaa tgcaggcagg acctaaataa gttgaataac aatgtatgcg
      121 tttcaataca cgtttgcctt gttgtcaatt tatttagttt ctgcctgttt tttgtgttga
      181 ggatggccac ttcgactcaa aagttcatca ctcaaaaaag aaaagaggga aacaccatga
      241 attacaaaca aacagcagaa gaagtattgc agtacatcgg tggggaagaa aacatatccc
      301 atttggagca ttgctcaact cgtttacgtt ttacgttaaa agacaacagc aaggtaaata
      361 tgagccagtt agaaaaagtg gatggcgtta tgggcgttcg ccaaaatgtg caatgccaag
      421 taatcattgg aaacgatgtt gttgaagtgt atgatgaagt gaaagcgtta ttaggtgaca
      481 atgtttcaaa tactgaagaa gcaccaaaac aaaaacaaaa attaggttca gttctattag
      541 attttattat ttctattttc caacctttaa ttcctgcgat tgcaggtgga ggagtattga
      601 agtccttgtt gctactagct tctatcttag ggctgattag tgaccaatct caaacctatc
      661 agattttaaa tttggtcggc ggggcaccgc tttatttctt gcctattctg gtggcaatga
      721 ctacggctaa taaattgaaa gtcaatcaat tggttgcagt atctgctgtc ggtgcattga
      781 tccttcctga aatgacagct atgttggcgg aaggtgctac attcttatct tttggattgg
      841 ataatatagc gtatgcatct caagtattcc cagccatctt aactgtttta ttttatgctc
      901 aacttgaaaa atggtttaca aaagtttcac caaaaccaat tcgtattttc tttgtaccta
      961 tgatgagctt attgattaca gttcctattg ctttgttgct tctaggacca ttaggttata
     1021 atgtaggaac agctttctct tctatcattg tgtctttgta caatcaattt ggatgggttg
     1081 caaccggagt cttagcagct gtactgccat taatggttgt aacgggtatg cataaagcaa
     1141 tgatccctta cgctgtttca tccatgagtg aattgggtgc agaacttctt tatctcccgg
     1201 cttcattggc acacaatatt tcagaagctg gcgcatgctt tgctgtcagt atcaaaacaa
     1261 aagacaaaaa attgaaatct acggctgttt ctgcgggtat atctgcatta ttcggaatta
     1321 ctgaaccagc tctttacggg gtaacgattc tgcacaaacg tgttttatat gccgttatgt
     1381 tttctagcct gatcggggga gcatttgcag gaatcgttgc acttcaagcc tttgctttag
     1441 tgggtcctgg gattgcaagt atcacgatgt tcgctgacaa agctgaccca atgaatctgg
     1501 tttgggcatt tgcagtatcg gggatttcat ttgtggttgc ttttgctgca gcgttggttc
     1561 tttataaaga taaagaagtt gtcaatgaag ttgaaactaa gatggaaacg gccgttgttt
     1621 cagatgctgt tctttcaagt cccgtgaatg gaaaagtaat ctcactatca gaagtaaaag
     1681 acactgtttt ttctagtggt atggtaggtg atggagtagc aatcattcct gaagaaggga
     1741 tattagtagc tcctgaagat ggagaaatca caatggtatt tgaaacgaag catgctattg
     1801 gtttaaaaac ggctaatggt gcagaacttc tttttcatat tggtattgat actgttcagt
     1861 tagaaggaaa agggtttgag gcatttgttc gttctggtga aaaagtagcc aaaggacaaa
     1921 agctgattgc atttgatatt gatttaatca aagaagcagg attcgacccc acagttatct
     1981 gtgtggtaac caataaggat aattacacta tccgttcatt taacctagaa aaattggtaa
     2041 ccaatgatag tgatctctta gctatcagtc cgatagctta aacacaagat agtgaaaaaa
     2101 aatatattga atgaaggaat agaggagaat agaataatga caaacacaac aagttttcca
     2161 aaaggatttt tatggggcgg cgcgatagca gcaaatcaag tagaaggtgc ttggaatgta
     2221 gacggtaaag gtctttcagt agcggatatg gctacttata aaccaaattt agacaacaaa
     2281 gattttgctg ctcatatgca tgtcaccctg cagtctataa aagaagctag tgaagacaaa
     2341 tctgataaat ggtacccaaa aagaaggggc gttgattttt atcatcacta taaagaagat
     2401 ttagctttat ttgccgaaat gggctttaaa accttgcgcc tctctattgc ttggactcgc
     2461 ttgttcccta caggagaaga gctggaaccg aatgaaaaag gggttgcatt ttatgaaaac
     2521 gtctttaaag aaatgcaacg tttaggaatt gagccgattg tgacactatc tcattatgaa
     2581 atgccattag ctttagcagt taaatataat ggctgggtgg agcgtcgagt aattgatgat
     2641 tttgttcgct tcgctaacgt ttgttttgat cgcttcggca aatacgtaaa atattggctg
     2701 acatttaatg agatagacag catccaccgt catccattta caacagctgg aattatccaa
     2761 gagaaaagcg cagaaggtca agaagctcaa gatatctatc aagcattgca tcatcaattt
     2821 gttgcgagtg cgttagtgac aaaagatgct catgaaaaaa ttccaggaag ccaagttggt
     2881 tgtatgttaa cgaaattgat gacttatcca ttaacttgcg caccagaaga cgttgagctg
     2941 accttgaaaa agaacttaga aaataatttt tatgcagatg ttcaagttaa gggagaatat
     3001 cctgctctaa tcgaaaagga tttggaaaat cggggaattc agattaaaat ggaagatgaa
     3061 gatcgagaaa ttttaaagaa acataccgta gattttgttt ctttcagcta ttacatgtcc
     3121 atggcagaat cagctgatcc aaacgcagaa cgcacaccgg gaaatactgt tttaggggtt
     3181 aagaatcctc atttgccttc aacagattgg ggctggcaaa tcgatccaaa aggtttgaaa
     3241 atttcgttga tcgaacttta tgatcgttac caagtaccgt tgatgattgt ggaaaatggt
     3301 atgggagcag tcgatcaggt agtagatggt aaaatccatg atccgtaccg catcgactac
     3361 tttaaagcac acttccaaca aatgaaagaa gcaatcgatg aaggtgtaga tttgatagct
     3421 tatactagct ggggaccaat tgatttagtt agtgcaggaa cttctcaaat gtctaaacga
     3481 tatggattta tttatgtgga tgcagacgat gaaggaaatg ggacatataa ccgcttgaaa
     3541 aaggattcgt tcaactggta caaagaagtg atcgaaacaa acggtgcatc cttattcgag
     3601 tagtctcgat ctttttaagc agcgataggg ggataatgag tgatttcaat aaaaaaagta
     3661 ttgaattcaa gtgtggtact tgttgaagaa aatggcaaag aaatgattgc attaggtaaa
     3721 gggataggct ttggacagaa agctggggat gaaatatcag gagataaagt agataaaata
     3781 tttctgcctt ttgaagaaaa gaaatcttca caactattag agttagttga agaaatacct
     3841 attgaatttt ttgaaatcac tcgtactatc gtttttgaag cagaaaaagg attagataaa
     3901 aaactgaatc ctggagtcta tttaacctta acagaccacc tgcattttgc agttgaacgc
     3961 gcacaaaaag gggtaaatat tgcaaaccgt ttgtactggg aaataaaaat ctactatccc
     4021 aaagaatttc aaattgggaa gtacgcactc acccttttga acgagcaata tggaattgat
     4081 cttcctaaag aggaagcctc aaacattgct ttccatttga tcaatgcaca atcggaagat
     4141 cctgatagcg gcgctggatt caagtatgca aaaatgatcg gcgggatcgt caatttggtc
     4201 cggtattcgt tgcagaaaga tatcgatact aattcgatcc attatgcccg cttcattaca
     4261 catgtacgtt tctttgtaga aagatattat tctgatgctt tgttggatga tggtgaggaa
     4321 gaattctaca atcaaatgtg gatcttatac ccgtctgcca tggaaagcgc agctaagatc
     4381 aaagactaca ttgaaaaagt ttatcataaa acaattccga acgatgaagt tgtataccta
     4441 ggtgtccaca tcaatcggtt gatgaaacat gctaatgggt gagcgctcag taatactatt
     4501 gatgctttta aaatctttag agatggatta tagtaaaaac aaggtgattt ccagctgttt
     4561 gttatctata agagacattt aaaaaggatt aacgtaagat aataaaaaat ct
//
LOCUS       DACENJ010000184         4573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.190, whole genome shotgun sequence.
ACCESSION   DACENJ010000184 DACENJ010000000
VERSION     DACENJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.190"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(165..1451)
                     /locus_tag="IW748_002250"
     CDS             complement(165..1451)
                     /locus_tag="IW748_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1105149.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1444..2712)
                     /locus_tag="IW748_002251"
     CDS             complement(1444..2712)
                     /locus_tag="IW748_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1105150.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2964..3425
                     /gene="mscL"
                     /locus_tag="IW748_002252"
     CDS             2964..3425
                     /gene="mscL"
                     /locus_tag="IW748_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1105151.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3484..3930)
                     /locus_tag="IW748_002253"
     CDS             complement(3484..3930)
                     /locus_tag="IW748_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1105152.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4023..4268)
                     /locus_tag="IW748_002254"
     CDS             complement(4023..4268)
                     /locus_tag="IW748_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105153.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4382..>4573)
                     /locus_tag="IW748_002255"
     CDS             complement(4382..>4573)
                     /locus_tag="IW748_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HAQ1105154.1"
                     /translation="KEAIENCEIQFSKIEAKIFHYYMQGVDFVDMAECLAMDHKQVMS
                     AYGRVKLKIKKHLKFYNEE"
BASE COUNT         1312 a          924 c          786 g         1551 t
ORIGIN      
        1 ttgattttat tttaatataa gttgattaaa agagagcatg atttgtttta ttttctcact
       61 aatttttcca acacaactaa gaataccata aaagctgatt ttggaggagg ttcctactac
      121 aagtctaaaa cgaaatttaa gacttttcct cataaaatac tggtttattt tggtctcatg
      181 taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag tacgtctttc
      241 atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc aaataaactt
      301 tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata ttgtccaagc
      361 attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc agcgaatcca
      421 agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc tccgctgaag
      481 tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc atcgataatt
      541 ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat ttggaacaac
      601 agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa tgtgtctaat
      661 cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc actttgtttg
      721 atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg agggaaattt
      781 tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat cttaccaact
      841 acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata caagtcttga
      901 gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa tgggtgtgta
      961 ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc ttcatacatt
     1021 tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt gaagtaaggt
     1081 gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt cgtagaaaac
     1141 agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg tttcccaaat
     1201 aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa atgggcaatt
     1261 ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt atcgatcgaa
     1321 ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg caacaaatac
     1381 acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg ctcatattct
     1441 gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt cgatttgctc
     1501 agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc attcttcatc
     1561 agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg ccgcttgcgg
     1621 agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag acagttccaa
     1681 ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat gaagtacttg
     1741 atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga acgtatccac
     1801 gctactagag gcataataag ctagactttc tttctctctg acattcatga aaagtttaga
     1861 gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt ctggttcatc
     1921 gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg gctcacgtac
     1981 ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg gataaactgt
     2041 ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg gatcaacatc
     2101 gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt aggacgctag
     2161 cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga tcttttgttc
     2221 cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt gtttatcttc
     2281 cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg tcaactgatc
     2341 aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat ctaccgcttg
     2401 agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc ctgcgtttag
     2461 ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat aaagctctgc
     2521 aagttgcgca cttaactttg tctgatccgg ataattcaaa ctatttgttt ccaacatgct
     2581 gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa aacggataaa
     2641 aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc cttctgccaa
     2701 ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac tcttttaatg
     2761 ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca ggcaattcgc
     2821 cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt atcaaaagaa
     2881 acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat actaaagaca
     2941 aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc atgcgtggag
     3001 atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc atcgtaaacc
     3061 aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg atcacaggta
     3121 cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa ggcgtcacat
     3181 ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt tttgtcttat
     3241 tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa gagcaagtgg
     3301 aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat cttcttgcca
     3361 aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc gatacttcta
     3421 aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc tttttttaga
     3481 ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat cttccaaata
     3541 cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt attcttgtgc
     3601 gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg tttcttgaac
     3661 aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac gtccaaaatg
     3721 gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat cagtatcttg
     3781 aaaaaacgta tgccaagctt gtccatcaat gtcatcaact tcttgataag gacactgttg
     3841 ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg ctgttgacaa
     3901 ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt ttttattctt
     3961 agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatctcagg ctcgtttgct
     4021 ttttaaactg cttcagttaa aaattcgatc tcaactgtcc gagtaagtac atctgaatag
     4081 ctgtaagata ctcgttcaaa agaattttct tcaggatcca aatcaacaac gaatactgaa
     4141 ggataggttt ctgttaaaac gccgcggcgt tctgtttgac gctttcttcc tgtctgagca
     4201 accaacatga ttgggctgcc gatacgacct tctaaatctt tcttgattga agctaaagtt
     4261 gttggcatac agttcacctc tactatcaac tataccacat atttttaaaa ttttcaagtt
     4321 taccataaaa aaaaagaaat gcaataaaaa taaataagaa gctcattaag atttttctaa
     4381 attattcctc attataaaat ttcaagtgtt tttttatttt gagttttact ctcccgtaag
     4441 cgctcattac ttgcttgtga tccattgcta aacattcagc catgtctaca aaatcaacgc
     4501 cttgcatata ataatgaaaa attttagctt ctatttttga aaactggatt tcgcaatttt
     4561 cgattgcttc ttt
//
LOCUS       DACENJ010000185         4505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.191, whole genome shotgun sequence.
ACCESSION   DACENJ010000185 DACENJ010000000
VERSION     DACENJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.191"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..851)
                     /locus_tag="IW748_002256"
     CDS             complement(<1..851)
                     /locus_tag="IW748_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1105155.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQP"
     gene            complement(853..2292)
                     /locus_tag="IW748_002257"
     CDS             complement(853..2292)
                     /locus_tag="IW748_002257"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1105156.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2477..4423
                     /locus_tag="IW748_002258"
     CDS             2477..4423
                     /locus_tag="IW748_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1105157.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1427 a          772 c          751 g         1555 t
ORIGIN      
        1 attggttgtc ttattgttgt caattgtggg aaaaatttct caataaaaga ggtaccatcg
       61 tatccaataa tttttaaatc ttctgggata ttaagtttaa gttgatgagc aattttcata
      121 gtgagaatgg ctgttaaatc atcagaaaca aaaataccat ctggtttgtg atttataata
      181 gttgacttta tttccatttc acgacggata gtagacagat tattaggaat tttgaaaatt
      241 tttccatttg gattttgaaa agaaaaacct agtgcacgta attctgtggg agaatccgtg
      301 ttatcatttc ctgtaatcat aatggtattt tgacaaccat ttttttgtaa agtcttagct
      361 gcaagttttc ccccttcaaa gttatctgaa gaaacaatgg aaacattagg agctaaattt
      421 ctatcaaaag caacaatagg tgctctcaca cgctcataat cagcaattcc aagattatgg
      481 cttgatgaga ttatgccatc aacttggttg gcttcaagca tttcaatgta ttctctctct
      541 ttagcaggat cattttgact attgcaaata atcgttttat atccatgttt aaataactct
      601 atttcgagat actcaatgag ttcagagtaa aagatattac taatattagg aaaaataaga
      661 ccaataagct gaggtgactt tccttgaagt ccacgtgcta aattatttgg tttataacca
      721 agagttttca tagcttcatg aactttttgt tttgtttttt ctgataaata tcccttatta
      781 ttaatgacgc gtgaaacggt tgttgggcta actccagcta attcagcgac atcagttaat
      841 tttgctacca ttttaatatt ttaattcaaa gtattttcca gtaggttcgc ctgatttgat
      901 ttgaatacca ttttggtgat tttcagggaa tacacgacta gtaaacactt tttctccttt
      961 attaataaaa atctcaatga ttgagttatc tacaaaaatg ttagcagttg tttctttagt
     1021 atcaattgag cattcacgaa tcgtaccaaa gtcagtggca tattgaatgc ctgctttgct
     1081 gcgatctaaa ataatttttc cttctttggt atctacaatc aagctaagtc catttccttt
     1141 actatcagag aaaagaagta actccgattt ctgatttgga ggaaaagtta gttcaagctc
     1201 ataagtatta tttgtttctg cttttgctgt aaattgttca gaactagcac gtaatgatgt
     1261 aatagcttct actggatatt ggtaaagttt accatctttt attgaaagtt cttttacaag
     1321 actcattgct ccctgataat catacttatc agttggataa tcgatatcag gtagtccaat
     1381 ccaactgaca atcagagcac gaccatcagg agtattgaat ccttgagtcg catacgcttc
     1441 aaatccataa tcaagatttt gaatttctga agcgcccaca agttttactt tttctatgtc
     1501 aagcgattga cagatcttaa aagtattcgg atagatattc ccataattta gctcagattt
     1561 atccaaccct tgaggacaat aaatgagaac aggttttttg tctacaaata ctagatttgg
     1621 acactcaatc atgtattctg aaccagtgcc gccaaaatca aggttgccaa cttcctccca
     1681 tgtttccacg ttattgtcat ccgctttgta aagtttgata aagcctgatt tttctaaatt
     1741 ttgtgctcca acaatcgcat acaattgtcc ttcataatta aacatttgtg gatcacgaaa
     1801 atgatcagtt acatcagcag gtttatgaat aagaacattc tcacctttcg taatgttgta
     1861 gtctttatcc atccatgctc cgatttgaag tgtatcacgt tcccaatttt catttcgaac
     1921 gtttcctgta tagagtaaga atagattccc ctctatttca tatgcagaac cggaataagc
     1981 gccgtggcta tctcgttttg tgtctggaag aagttctgct cctgttacat gaaaatgaac
     2041 taaatcatct gattcattgt gtacccattc tttcaaccca tgagcagctc caaatggcca
     2101 attttgataa aaaagcgtat attttccgtt aaaaaaagaa aaaccattag gatcgtttaa
     2161 caatcctgtt ttaggttcga tatgatagct agcacgccaa ggtgatcgtt ttacattatc
     2221 cttaatttta tccatctcat ctttagtcca atcagaataa gcacgatatc gtatttctcg
     2281 aggtaagttc atattttact ccttcaattt atctttttac aactagtatg gtaaacgttt
     2341 tccgttgaaa aatcaatcat ttcatttaaa aaaataaact atatgacaaa cgtttgacat
     2401 atttttaaga tatgctaaaa taaatttgca tttcagtgaa ccgttttcac gtaaaaataa
     2461 aaaaggagtg attcgaatga attacagtaa agtagcttcc gacgtaatac aagcggtcgg
     2521 aaaagataac cttattgcag ctgcacactg cgctacacgt cttcgtctcg tactaaaaga
     2581 tgacacgaaa gtgaatcaaa aagctttaga cgaaaatcca gatgtgaaaa gcacatttaa
     2641 aattgatgga caatatcaag tgattatcgg tgcaggtgat gttaatttcg tttatgacga
     2701 acttatcaaa aaaactggac tttcggaaat ttcaacagat gatttaaaac aaattgctag
     2761 taaagataaa aaattcaacc ccattatggc attaattaaa ttattatcag atatttttgt
     2821 tccaattata cctgcccttg ttgcaggtgg tttattaatg gcattacaaa actttttaac
     2881 agctgctgga ctttttggac caaaatcaat tgaagaaatg tatcctgcga ttgaaggtat
     2941 ttcctcaatg atccaactga tgtccgctgc accattcatg ttcttaccaa ttttagtagg
     3001 tatttcagct gctaaacgtt ttggagcaaa ccaatttctt ggtgctgcaa ttggtatgat
     3061 catgacaaca ccagatcttg gtggctcaac tgaatattgg aatatttttg gttatcatgt
     3121 ttcacaaaca aactatgctt atcaagttat tccagtttta gcagctgttt ggatattatc
     3181 tatattagaa aaattctttc ataaaaaatt gccatcatct attgacttta cattcacgcc
     3241 attattatca gtaatgatta ctggattttt gactttcaca gttattggcc ctgtaatgtt
     3301 attgttgtct aatggaatta cagacattat tgtttggcta tataacacta ctggatttat
     3361 tggaatgggt atttttggag gaacatattc acttatagtc atgactggtt tacatcaatc
     3421 atttccagct attgaaactc aattactttc agcgtggaca aatggtatcg gacatggaga
     3481 ctttatcttt gtagttgcat ctatggcaaa cgttgctcaa ggtgctgcaa catttgccat
     3541 ttggttctta actaaaaatt ctaagacaaa aagtcttgct tcctctgcgg gtgtctcagc
     3601 acttcttgga attacagagc ctgccttgtt cggagttaac ttaaaatacc gctttccatt
     3661 cttttgtgct ttaattggtt caggaattgc tacagcagta gcaggattac ttaaagtagt
     3721 tgctgtttca cttggttcag caggtttctt gggatttctt tcaattaatg caacatctat
     3781 tccattttac gtattgtgtg aattgattag ctttgtagtt gcttttgcaa ttacttactt
     3841 ctacggtaaa acaaaagctg ctggtatctt taaagcagaa gaaattgctg aacaaacacc
     3901 agttaattct tcaaaagtag caacaacaga tccaatatct actgatgttg aatcaacaca
     3961 aacaaaagtt tcatcagaaa caattgttag tcccatttct ggtaaagtga ttagtctttc
     4021 ttcagtaaat gatccagtct tttcatcaga agctatggga aaagggattg ccattaaacc
     4081 aaatggaaac acggtccatt ctccagtaga tggtactatt caactcacgt ttgaaactgg
     4141 acatgcttat ggtattaaat caaataatgg tgctgaagtt ctaattcata ttggtattga
     4201 tactgtatca atgggaggaa aaggatttac acaaaaagtt actactaatc aaaaagttag
     4261 aaaaggcgat attcttggag catttgaccg ttcaatgatt gagaaagaag gtcttgatga
     4321 tacaacaatg attattgtga ccaatacttc ggattattca gaagtaaata tagtagctga
     4381 aaatacagtc actgagggta ctgatttact tgaaatcaaa taaatatatt tccctgagta
     4441 aagatggaat tttctttcca aaatcaaata atatagatta atcaaatatt tgagaataac
     4501 atttt
//
LOCUS       DACENJ010000186         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.192, whole genome shotgun sequence.
ACCESSION   DACENJ010000186 DACENJ010000000
VERSION     DACENJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.192"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW748_002259"
     CDS             complement(543..2048)
                     /locus_tag="IW748_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1105158.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW748_002260"
     CDS             complement(2045..2731)
                     /locus_tag="IW748_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1105159.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW748_002261"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW748_002261"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1105160.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACENJ010000187         4390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.193, whole genome shotgun sequence.
ACCESSION   DACENJ010000187 DACENJ010000000
VERSION     DACENJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.193"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(20..1576)
                     /gene="rny"
                     /locus_tag="IW748_002262"
     CDS             complement(20..1576)
                     /gene="rny"
                     /locus_tag="IW748_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1105161.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1987..3036)
                     /gene="recA"
                     /locus_tag="IW748_002263"
     CDS             complement(1987..3036)
                     /gene="recA"
                     /locus_tag="IW748_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1105162.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3145..4386)
                     /locus_tag="IW748_002264"
     CDS             complement(3145..4386)
                     /locus_tag="IW748_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1105163.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT         1130 a          997 c          766 g         1497 t
ORIGIN      
        1 tcttcttttg tattttttat tatttagcat aatcagttgc acgagtttcc cgaataacga
       61 ttactttgat gtgtccagga taatccagtt cttcttcgat tcgtttacga atatcacgta
      121 ctaaacgaac agcgtctaaa tccgagattt cttctggctt gaccattacc cgaacttcgc
      181 gtccagcttg aactgcgtaa cttgattcta caccggcgaa gctgttcgaa atattttcca
      241 gacttcttaa acgacggatg tagttttcta atgattcact tcgcgcacca ggtcttgctg
      301 ctgagagcgc atctgctgca gctacaagga cagagataac agaagtagct tccacatctc
      361 catgatgaga agcgatcgcg ttgattacta ctgggttttc tttgtacttg gcagctaatt
      421 ccgcaccgat ctcaacgtga gatccttcaa tctcatggtc aagtgctttc ccaatatcat
      481 ggagcaaccc ggcacgtttt gctaattgga tatcttcgcc aagctctgct gctaagatac
      541 cagctaattt tgcaacctcg accgaatgct tcaagacgtt ttgtccataa ctagttctga
      601 atcgcagacg tccgatgatc ttgattagat ctggatgaag tgtatgagca ccgacttcaa
      661 acgctgcctc ttccccgtat tcgcgaatac gttcatccat ttctttacga gatttctcga
      721 ccatttcttc gatacgcgct ggatggattc gtccatcttg tatcagtttt tccaaggtca
      781 tccgtgcgat ttctcttcgg attggatcga atccggaaag aacaactgct tccggtgtat
      841 catcgatgat taaatcgata ccagtcagcg tctcaagcgt tcgaatatta cgaccttctc
      901 gtccaatgat acgacctttc atttcatcat tcggaagggt aacgactgaa acagtcgttt
      961 ctgaaacttg atctgctgcg cagcgttgaa tcgctaatga taataagttt ttcgctttgc
     1021 gatctgcttc ttctttggca cgctgttcag attcttttac cattagagtt aactcatgat
     1081 tcagctcttc ttcagtcgat ttcatgatga catctcgtgc gtcctctttt gataaaccag
     1141 caattttttc tagttctttt tgttgatctt caatcatttt ttcaacgtct ttttctcgct
     1201 cttcaattaa ttgctgtttt gaactaagtg cttcttcttt actttcaagt gagttctcgc
     1261 gtttttctaa cgagtcatct ttacgattca gcgtttgttc gcgttgaatc aaacgattct
     1321 cttgagattt taattctaac ttactttctc tcagctcact ttcaacttca gaacggtatt
     1381 tctgattttc ttccttagct tctaacaatg cttctttttt tagagtttct gcttcttttt
     1441 ttgcactatc gatgattcca gcagctgatg attgtgcacc agctatttct ttctcgtgac
     1501 gtgactttgc gaccataaac cctaaaccaa gaccgacaat taaaccgatg atagcgagga
     1561 gaatgttgaa taccataaaa tccacctcca tactatcttt ttcattttta tcatctatac
     1621 agcttctatg ataaattatt ctaaactcgt caatatgcat cttcgcatat atgtgtacaa
     1681 aaaacatgca caactttatt ctaatgtttg taccccgtag tgtcaacttt aaataaacaa
     1741 agaaacggct ttctacttat tcattaaacg atggttttta aaataaatga cactagtttc
     1801 atataggtaa acctttaaat tgagaaactt ttctattaaa ttattctttg tagatgaaca
     1861 gatattttta ttttatgtac ccactcgtat aattaaataa ctcacgattt ctttattcaa
     1921 taaaaaaacg gcaggtcaag gagagtacct actcttcaga ccagccgttt ttgtttatca
     1981 taatttttat tcttctacta aaggcaattc ttcttgtcct ttttcatctt ctgggacagc
     2041 gacttcttca ccaataccat aagctgctct aactagagca gaaacttctg ccatcatttc
     2101 aggatgatta gccatataga ttttagcgtt ttcccgtcct tgaccaatcc gatcttcttt
     2161 ataagaatac caagcaccgc ttttatcaac aatgtctttt tctactgcca tgtctagaag
     2221 ttctccttct tgtgagatcc ctaaaccata catgacatct acttcagcga ttttgaaagg
     2281 aggtgctact ttgtttttta cgactttgat ctttgtacgg ttaccaacaa tatctgtacc
     2341 ttgtttcaat tgttctgcac gacgtacttc caaacggatc gttgcgtaga atttcaatgc
     2401 gcgtcctcca ggagtgattt ctggattccc aaacatcacg cctacttttt cacggatttg
     2461 gttgatgaaa atagcgattg tttttgtttt gttgatcgaa ccagagagtt tacgcaatgc
     2521 ttgagacatc aaacgtgctt gtaacccgac atgtgagtca cccatttcgc cgtcgatttc
     2581 tgctcgtgga actaaagcag caactgaatc gactactacg atatctacag ccccacttga
     2641 tactaaagca tcagcgatct ctagaccttg ttctcctgtg tcaggctgtg aaagaagtag
     2701 ttcatcgata ttcacaccta atttttgtgc atattgcgga tctaacgcat gctcagcatc
     2761 aatgaaagcg gccgttccgc catttttttg tacttctgca atagcgtgta gtgcaactgt
     2821 tgttttacct gaactttcag gtccatatac ttcgatgata cgtccacggg ggtaaccgcc
     2881 gacacctaaa gcgacatcta acgcaagaga gccacttgga attgtagaga tttgttggtc
     2941 gattttttcg cccaatttca taatagagcc tttaccatag ctcttttcaa tttttttcag
     3001 tgcagcatct aaggctgctt tacgatcatc tgccaatacg ataatcctcc ttatatttac
     3061 aaatcagagc aacatttcat tatccttatc atatcttttt tagtgaaaaa aagcaagaaa
     3121 aaaccgaaca aatattcgca tttatcattt tcttaaaagc tgttttctca aaagatcaaa
     3181 tccttgcata accgcattat atctgatagc agcacgatct ctggcaaagt gatatttttc
     3241 cgaaaccacg ccgtctttag atgaaatagc gatccatact gttcctgcag attgaccttc
     3301 taacgtgtcc ggtccagcca ctccggtgaa agctacactg taatctgttc ttgctatctc
     3361 acgtgcttga atggccatct gctccacaca ttctttgctg actgttccat attctgctaa
     3421 tagctccgga tcaatctcca gaaaaccagc ttttgtttgg agcgagtagg taacgaatcc
     3481 gcctggaaat acttcagata caccagcaat gtcgcctaat gcggcttgga aggcgccggc
     3541 tgtcaaactt tccgctgctg ttacagtctg cttgttttct ttcagtagtt ccactacgac
     3601 ttttgctagc gaattatcat cgccgtatcc ataaaaatat tcccctactc gttcttggat
     3661 tttttcttct aatgctaaaa gagcttgatt ccctgcctga acatcatttg ttttcactgt
     3721 cagtcgtaat gtcacttcat ttggtttggc atatggtgcg atcgttggat tgatctgtgt
     3781 ctcaatcaga tcttttagct cagtgaccag tcgcgattca ccaatcccat aaaagcgcaa
     3841 aactttagaa attagttttt cttcagaagg aaaacgttcc tctagtaaag gacgtacttg
     3901 ttccacaaac attggtttca actcattagg cggaccggga agaaggatat acgcatgtgt
     3961 atctgtttga taaaaagtcc ctaaagcaag ccctgtacga tttggtagag gaaccccgcc
     4021 ttcgataatc tgggactgtt gcagattgtt tttggtcatt tttcgatggg ttgtttcaaa
     4081 atatgccaat aattttttgt atccttccgt attttgtatg agagacttac cgaggtgagc
     4141 cgcagttgct tcttttgtca ggtcatcttc tgtcggtcct aatccgccac ataagagtat
     4201 gagttcactt cttgtttcag ctagagcaat caactcttct aaacgctgct gattatctcc
     4261 tacaactgtt tgataataaa cttcgatacc tagatcagct agctgttctg acaagaaagt
     4321 ggcattcgta ttcacgactt gtcctagtaa aagttctgtt ccgacagcta ttatttctgc
     4381 tttcattact
//
LOCUS       DACENJ010000188         4363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.194, whole genome shotgun sequence.
ACCESSION   DACENJ010000188 DACENJ010000000
VERSION     DACENJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.194"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..235)
                     /locus_tag="IW748_002265"
     CDS             complement(<1..235)
                     /locus_tag="IW748_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HAQ1105164.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEE"
     gene            379..729
                     /locus_tag="IW748_002266"
     CDS             379..729
                     /locus_tag="IW748_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1105165.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            710..1426
                     /locus_tag="IW748_002267"
     CDS             710..1426
                     /locus_tag="IW748_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1105166.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            1426..2874
                     /locus_tag="IW748_002268"
     CDS             1426..2874
                     /locus_tag="IW748_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1105167.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2944..3453)
                     /locus_tag="IW748_002269"
     CDS             complement(2944..3453)
                     /locus_tag="IW748_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105168.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(3443..4168)
                     /locus_tag="IW748_002270"
     CDS             complement(3443..4168)
                     /locus_tag="IW748_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105169.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1518 a          735 c          887 g         1223 t
ORIGIN      
        1 tttcttcgcc gtctactcga atgaaagaac cattcatggt gaccgcagaa gtaatgcccg
       61 catcttccat gatagagtgg atttctgctt ctgtacgtcc tgtagcgatg acggggagaa
      121 cattgttttc ttttaatgcg ttcatagctt ttgccacttc gggtgtgatt tttgatttcg
      181 catctagtaa tgttccatca agatcaaaga aagtgattgc acgataattt gacacaaata
      241 aaactccttt tttattggaa taagtttatc ttaacaaaga atgaacaaaa gacaaacctt
      301 tcgtgaagaa ttcctgaaca tattggttca gtcaacaatt tagagtatac tataagaaga
      361 gaatttagga gggaaagaat gatttttttc agtattggta tcttattctt aataattggc
      421 agtctttttc ggattctgcc ctcaaaagga aatctgcctt tttatgggta tcattcgcct
      481 cttgccgcaa aaacagacgc tcattggcgg ctggctcaaa agacaagcgg aaactggttt
      541 tttcttatgg gcttactgat ggcattgatc ggctattatt taaagacatc tgggcatacg
      601 aattattttc tgattgaaat gcttctgcta gtatttccaa ttatgcctat ttttattatg
      661 acagaaaaga aattacagaa atatgacctg gaaactggag ggaacgataa tgaatatttt
      721 aatgattgaa gataatcaat ctgtttctga aatgatgcag atgtttttct taaatgaagg
      781 ttgggaagca actttcaagt atgatggaaa agaagggctc gatgcctttt tagaaaaccc
      841 acaacattgg gatatgatta cactagacct gaatttgccc acactggacg gtatgctagt
      901 aggaagagag atccgcaaag tttcaagtac tgtgccgatt atcatgctga cagctagaga
      961 ttctgaaagc gatcaggtga tcggcttaga gatgggggca gatgattatg tcacaaaacc
     1021 tttcagtcct ctgacgctta ttgctcgaat gaaagctttg catcgccgta gtgagttggc
     1081 agaagcaaga gaagaagcag tagtttcaga tgatcatttt gatatcgaga ctagtcattt
     1141 caagatgaat acgaaaacac gagaagcgta tctggacaac cggccaattg aaggactgac
     1201 gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa ccacgtcaag tattctctcg
     1261 ggagcagttg cttgaattag tctgggatta tcagtacttt ggtgatgaac ggacagtgga
     1321 cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc ggaccacaag tcatccaaac
     1381 tgtttgggga gtcggctaca aattcgatga ttctggtgtt gcctaatgcg ttatttgtat
     1441 cagcaactct tagctttctg cggaatggtc gcgttgatct tattgatcgt tggagtttct
     1501 tttacacaac tgacaaaaca aacgattgaa gaaaacaatt accagcaact gtttggatat
     1561 gctgaatcag ttgaaaagac aacgcaaacc tatgccgata cgctcccgca gtttagtcaa
     1621 gatcaggcgt ttcaaaatgc attgctgctg acagagcaag tactgacaga acaaaatgtg
     1681 aactttatat ttatcgataa aaacgaacgt gtcatttatc cgactaatac agctttactg
     1741 aattttacct taacagctga tcaatggaaa aatctgagga atggacgtca agaaaaattc
     1801 acatcgaata aaaatatttt aggacaagca caggcgacct cttacgcact tgtgccattt
     1861 aatttgaatc atgaattcta tggagcactt gtagttagtc aacctgcgcg aaatatcgat
     1921 aacagcgttc gtccaattac actgaacctg ttcaaaggat ttattttttc cagtatcgtt
     1981 gcagttattg ccagctatgc ttttgcagca ttccaagtta agcgtatcaa tcggttaagg
     2041 aatgcgacga aagaagtgac gaatggtaat tttgatgtac agctccccgt acatgacaaa
     2101 gatgaatttg atgagttagc agatgatttc aataaaatga cgaattcatt gaaagaatct
     2161 caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat ttatggcaga tgcttctcac
     2221 gagatgagaa cacctcttac gacaatcaac ggacttctgg aagggttaga atacaatgca
     2281 atccctgaaa atcagcgaga aaacgcgatt aaattgatga aaaatgaaac agagcgtctg
     2341 atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc gtacgaatca aatcagtatg
     2401 gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata ttgttgcaca gttagaagct
     2461 aaagcagaag cagcaggaga tacattgaca ttgaaagccg ttgatgatat tgatgtttac
     2521 gctgattatg accgatttgt ccaaatcatg gtcaatatta ttcaaaacgc gatacagttt
     2581 actgaaaacg gccaaatcac gattacttta gaaaaaggct atcttgaaac gatcatcacg
     2641 attgaagata cgggaattgg aatgtctgaa caacaaatga aaagtatctg ggatcgttat
     2701 tataaagtag atccttcaag aaaaaataca aaatatggtg aatcaggatt aggtttacca
     2761 atcgtgcagc aacttgtacg cttgcataaa ggaaaattag aagtagaaag cgaactagga
     2821 aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa tcaaaaaaga gtaaaaaaca
     2881 agcggtataa aagatcatga atcgatcttc tataccgctt gtttttacta taaaatgatt
     2941 gatttagccc gcgggctcta aacgatcaat ataaggactt tgagcaatag tagcatcatg
     3001 agtaaagagt aagatacctt gattttcttc atgagctaaa taagaaagca agtggagtag
     3061 ctcttggatc tccaatacac taagttcatc aaatgtatct tcaataacaa tattgtttcg
     3121 cttggaaagc aaggcatgaa gtaattgcag ctttactttt tcaaaagacg tcaactcagc
     3181 tgccgcacaa ttaagcaagg aaggagagat atcaagtttt tcttgatagg ccaaacgatc
     3241 ttgtttgatt tctttttccg gtacatccaa caggacatgg tctttaagat tcagataagg
     3301 aaacatcggc caattttttt ggatgatcgc aaaattatcc agttttgcaa caagaacctc
     3361 ttttttcaag tgctccaata ttttatgctg tttttcgaga ggacacaaaa taacggtcaa
     3421 gtgttcagca tgaatcaaat attcaggttt cattacgtcc aggcctccta ctttgcaaat
     3481 gatggataaa aaaagtgaaa gggaatagac taagaatgag acagcatcca ctgagcccaa
     3541 taagagtttg ggcaaatccc ttaagtatat ctgtgaaaaa aagatcattt tggaaatcga
     3601 tggtaaaaaa agtctgttga tccatcggaa ttgtaagtcc gcttaccgaa gtcaagatat
     3661 cacggatatc tggcagttca gtagtcttta agacaagaaa attgatcctt tgaagaagcg
     3721 cttcataaac tgactggaag cttacgatcc acaataaaac caagatacaa caaagaaaga
     3781 gaggaaagaa cagctgccag aaaataaaaa ggaataattt ggttttagaa agaccagtca
     3841 ggtgccaatt gatcaattcc tgcttgtttt gcctgactga atagtaaaag aaaaagataa
     3901 aaaggaaggc aaatagaaaa aataaactga atatgccttt tatataaaat cggctagcta
     3961 attctaaagc cggaagaagc tctgtagatg cagaagcgcc aataaatgaa agccgatctt
     4021 ggacttgatt ggctaaagcc gattgcaaat ggtaaagagt caggcatgct atcaataaaa
     4081 aagcagaaaa gcttgaaaat aaacaataga gtattgtctg ctttttgtaa taaattaatc
     4141 gagcagtagc atagcggatt attttcatac ttttcacctc tataaaaagt atagaaaatg
     4201 aatatgaata aagtatgaaa ttcctttagt gaaatcacga attaaaacca gaaaatattt
     4261 tgtaaaaagc aagaggttgt gaagaagctg ccctattcta ttaccgtaga tgcaagcttt
     4321 tgaacactgt ttattcgaaa agaggagcag cgacaataat ttt
//
LOCUS       DACENJ010000189         4352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.195, whole genome shotgun sequence.
ACCESSION   DACENJ010000189 DACENJ010000000
VERSION     DACENJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.195"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            382..1755
                     /gene="glmU"
                     /locus_tag="IW748_002271"
     CDS             382..1755
                     /gene="glmU"
                     /locus_tag="IW748_002271"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1105170.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            1849..2820
                     /locus_tag="IW748_002272"
     CDS             1849..2820
                     /locus_tag="IW748_002272"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1105171.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      2826..2928
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2994..3563
                     /gene="thiT"
                     /locus_tag="IW748_002273"
     CDS             2994..3563
                     /gene="thiT"
                     /locus_tag="IW748_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1105172.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(3644..>4352)
                     /locus_tag="IW748_002274"
     CDS             complement(3644..>4352)
                     /locus_tag="IW748_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105173.1"
                     /translation="ATDFANSIVHFPQNLLLLDPSASAGEYESHTGLKVIRGTENIQR
                     FFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISELLYFGHMKTHLHSPFFYKLQNNF
                     VYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKINVKKSFFKKETPVEKISVELLK
                     EMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIATLIYSVTD
                     RHWQLVEDADLSYLPLK"
BASE COUNT         1437 a          773 c          932 g         1210 t
ORIGIN      
        1 agaggttgtg aaatcagcgt cctgtcctga gtattagctc aataaacgga aaaattgctc
       61 ctcatatttt gccgtttatt gagctaatac cgaaggtcag ctgattgaaa aacgtttaat
      121 gaagaggttg taagcctacc gtttaactct aagtcacaag aaacgatttc aaaaaacagc
      181 ttttcatgtt ttatgagtag tgtgacttgt gaccgaggag ttggcaggag agcaccggtt
      241 accaaataga taggcccgtt atggtctatc tattttttta tccagaaaac gctgggataa
      301 aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg gataaactga aatcagcaaa
      361 ctatatttag gagtgacgca gttggacgca cgttatgcaa ttattttggc ggcagggaaa
      421 ggtacccgca tgaagtcaaa attatataaa gttttacatc ccgtttctgg taaacctatg
      481 gtagaacata tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat tacgatcgtt
      541 ggacacggag cagaacaagt taaagcacaa ctaggagaac gcagtaaata tgcgttacaa
      601 gcagaacaat tgggaacagg acatgctgtt ttgcaagctg cttctttctt ggaaggaaaa
      661 aaaggaacaa cgttagttat cagtggcgat acaccattat tgactactga aacgctgaac
      721 aatctttttg aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac ggcacaagca
      781 gaaaacccaa caggatatgg acgaatcatc cgtgaccata ttgggatcgt agaaaaaatc
      841 gtggaacaaa aagatgcaac accagaagaa gctcttgtac aagaaatcaa tacaggaact
      901 tactgttttg acaacgaagc tttgttcgat gcgttaagca aagttggaac aaacaatgct
      961 caaggtgaat actatctaac agatatcatc gaaatcttaa aagaagaagg ccatacagtt
     1021 gctgcttatc aaacggatga ttttgaagaa tcaatgggag tcaatgatcg gatcgcgtta
     1081 gctaaagcaa atgagatcat gagaaagcga attaaccaga tgcatatggt caatggcgta
     1141 agctttgtcg attcagcaac gacatacatc gatgcaggcg tagaaatcgg tccagacaca
     1201 ttgatcgaag caggtgtaca aatccaagga aatacagtca ttggttctga ttgtgtcatc
     1261 gggtcacatt caaaaatcgt agacagtagg attgaggatc acgtcgtcat tgaaaactca
     1321 gtcattgaaa gcagccatgt caagaaacat gcagatgtgg gaccttatgc acatttacga
     1381 ccaaaagctg aaattggcga aaacgtccat atcggaaact ttgtggaagt gaaaaatgct
     1441 caaatcggca aaggaactaa agtcggtcac ttgacttatg ttggtgatgc tacgctggga
     1501 gaagaaatca atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa gaacaaacat
     1561 catacgactg tcggcgacca cagctttatt ggttctagca cgaatatcat cggacctgtc
     1621 gaggtagcta aaaacagttc gattgctgcc gggtcaacaa ttacagacaa tattccagaa
     1681 tatgcgctgg caattgcgag agcaagacaa gtcaataaag aaggatatgc aaaaaaactc
     1741 ccttatctaa actaagactt ttgcaaaatc acttgattta tttaaaaaat atgactacta
     1801 tttattaggt agaattctag agttgtgaaa aagaaagtgg aggtccccat gtcaaaacat
     1861 tactttgacc caagattaaa aatttttgcg ttaaattcca atcgcccatt agcagagaaa
     1921 attgcagcag cagtcggcgt cgagttagga aaatcatctg ttactcaatt tagtgatgga
     1981 gaaattcaag taaacattga agaaagtatt cgtggatcgc atgtctatgt tattcaatca
     2041 acgagcagcc cagtcaatga taacttgatg gagttgctga tcatgattga cgcactgaaa
     2101 cgagcaagtg caaaaacaat caacgtagtc atgccttatt atggctatgc gcgtcaagac
     2161 cgtaaagcac gtgcacggga accaatcaca gcgaaattag tagcaaacat gattcaaaaa
     2221 gctggtgcaa caagaatgct gactttggac cttcatgcag tccaaatcca aggtttcttt
     2281 gatattccag tcgatcattt gatgggtgcg ccacttattg ccaattattt cttagaaaaa
     2341 ggaatcaaag gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt cacacgggca
     2401 cgtaaattag cggaattttt gaaatctccg attgcgatca tagacaaacg ccgtccaaaa
     2461 gcaaatgttg cagaagtaat gaatatcatt ggacaagtca aaggcaaaac ttgtgtactg
     2521 atcgatgata tgatcgatac agcaggtacg atcacattgg ctgccaatgc tttgaaagaa
     2581 gctggtgcaa ctagtgtgta tgcttcatgt acacatccag tattatccgg ccctgcttta
     2641 caaagaatag aagattcagc tatcgagcgt ctagttgtta cagactcgat ctatcttcca
     2701 gaagatcgta aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat gggagatgca
     2761 atcaaacgta tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa aaataaataa
     2821 taaataaact cacaaagggg agtccaattg ggctgagatt gaatctattt ctaaaccctt
     2881 cgtacctgta tcggttatgc gagcgtagga attgtgaata aacagctgtt tgactatttt
     2941 cgaaagctgc ttcctccttt gtgacttttt tattcaaagg aggaagtttt tttatgggaa
     3001 aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg ttgtcgttga
     3061 ttccgatcca gatcggcagc agtttttcta tttcattagg gcagattcca ttgactcttt
     3121 ttgcgttaag aagagggaca aaggcaggga tgctggcagg tttcatttgg ggcatcctac
     3181 attttccgtt gggacaagtt tattatttaa gtgtcgttca agtgttgatt gagtacccga
     3241 ttgcctatac ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa aaaggctatg
     3301 tagacgataa tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg attggcgcac
     3361 tttccagata tttttggcat tttattgcag gagtggtttt ctggggagcg tatgcactat
     3421 gggggatgaa ttcttggctc ttttctttcg taatgaatgg tgccagcggg ttgagtacag
     3481 ctattgtaac gatcatcgtt ttattaggga tccaacaaac gactccttcg atttttgtgc
     3541 cagataaacg acaggagctg taaataaaaa agaagcaaga aacttgagac agaatcatgt
     3601 cctagtttct tgtttccttt ttattttttg ataagaaata gatctacttc aatggcagat
     3661 aagaaagatc ggcatcttct accaattgcc agtgtcgatc ggtgacagag tagattaaag
     3721 tagcgatgat ttcctcttct cgataccggg tgccttttaa tttccaaata taatcttcaa
     3781 tcacatagat tgggatacga tatttaccat cgatcaaacc aacttgatca aaagaaaaaa
     3841 tgatcccttc ctgcatcaaa ttacgcattt ctttcaataa ttccacactg attttttcaa
     3901 caggtgtttc ttttttgaaa aaagattttt ttacattgat cttatctgac aagatcgaac
     3961 ttaatttact agcaaaaatc ctataaaatt catctaaata gcgataataa atccgattca
     4021 gctgatcatt aagatcgaag tatacaaaat tattctgtag tttataaaaa aacggcgaat
     4081 gaagatgtgt tttcatatga ccaaaatata ataattctga aatttctaaa ggtgtcaatt
     4141 ctttcagcat cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat ccacgcctgt
     4201 tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc acggattact ttcagccctg
     4261 tatgggattc atattctcca gcagaagcag atggatcaag caagagcaag ttttgaggaa
     4321 aatgcacgat actgtttgca aagtcagtag ca
//
LOCUS       DACENJ010000190         4345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.196, whole genome shotgun sequence.
ACCESSION   DACENJ010000190 DACENJ010000000
VERSION     DACENJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.196"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(151..1881)
                     /gene="spxB"
                     /locus_tag="IW748_002275"
     CDS             complement(151..1881)
                     /gene="spxB"
                     /locus_tag="IW748_002275"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1105174.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            2046..2864
                     /locus_tag="IW748_002276"
     CDS             2046..2864
                     /locus_tag="IW748_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1105175.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(2967..3164)
                     /locus_tag="IW748_002277"
     CDS             complement(2967..3164)
                     /locus_tag="IW748_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105176.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            3333..3911
                     /locus_tag="IW748_002278"
     CDS             3333..3911
                     /locus_tag="IW748_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1105177.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         1286 a          782 c          882 g         1395 t
ORIGIN      
        1 tacagtttta ctgttgtaat tggaagccgg cttttttatc atggacaaat atgcacgagg
       61 tgatttttag gtgtcgagac aaatttatgc aaaaatgtga gaagctatat acacaaattt
      121 tatcttaata ctcgaagcat agcgcagttg tcacaacctc ttttcatata attcattcaa
      181 tactggcaaa tcatggactt catatttttc tttgaatgct tggatttctt cttcagaata
      241 ttttcttgga tctaaatgca gatcttctac tggcaatggg cgcttgttat tgatcttcac
      301 atcgattact acaggacgat ctgattttgc agctgcatca aaggcaaatc gaagttgcga
      361 gtattctgtg actgtaaagc tatttgcccc gagcgcttct ccaactttac cgaaatctgc
      421 atcttctaaa aatacaccaa atttcttttg ttccgtatct tcttgttctg cttcgatgaa
      481 accaaaagaa tcattcgaaa agacaacatt aataattgga agctgatatt tgacttgtgt
      541 aatgatatct tgcatgacca tagcaaaccc gccatcacca cttaaggtaa atacttgttt
      601 ttctgggtag cttagttgtg cggcaattcc tcctggcaca ccattaccca tagttgcgaa
      661 ccatccagat gtcgtatgtt tttgtttacc gttcatattc aataaacgaa tagaatggat
      721 agtcgtgtta ccgacatctg tgacgaaaat cgcatcgtct tcagcaatac gattgatttc
      781 tttatagaca gcctctggac gaatcggttc ttcctctcga tcttcaaaag atttcaacca
      841 atttacccat ttttctttat tttcttgatt tgctaggtac cagctatcag cagggcgagc
      901 atcccctaac tcgacaagtc tttgcaaagc tgtgtttgca tcaccgagga tcgacaaaga
      961 tgtttcatgg cgtcgtccaa attttgacgc atcgatatcg atttggataa attcagcagt
     1021 tggattaaaa aatgcacgtc caaatgggaa gtcgctaccg acaaataaga tcagatcggc
     1081 ttctgccaat gcttcatttc cgggtttagt agcgacacgt ccagcaaaac caagaaagtt
     1141 ttcataatca tcaggaataa ttccttttgc tagaacggaa gcgacaacag gaactgaaaa
     1201 atgttctgag aattctttga tttgcggaaa tccgtttcgc gttccttgac caacataaag
     1261 aactggtttt tttgcttttt caagataagg caaagcagct agcaaatctt tttcttctgg
     1321 taatatcaca cttgttttgt gagtgtgagc ggtggagaaa ggttgttcgt caatttcttc
     1381 aaaaccaaga tcaacaggga tagtcacgat agccacgcct ttatgctcat aggcggcttt
     1441 aatggcttca tctactacat gcggaagact ttgtggtgtc atgactgtac gattgtatac
     1501 gctaacatct gcgaaaatag gattttcatt tagttcctgg aaagagttat agttcatgga
     1561 tgtagaagca acttgtccta agaacgctac tagaggcaca tggtccatct gtgcatcata
     1621 caaaccattg atcaaatgtg tcgcaccagg tcctgcagag ccgaagacag cgccgacttt
     1681 ccccgttaat tttgcatctg cagcggctgc gagtgcgccg acttcttcat gacgaacttg
     1741 aatgtagttg atttcggttt tttcttgata tagcgcatcc ataattgaat tgaaagatcc
     1801 tccgggaatg ccgtaaatat ggtcgatacc ccagctttca agaactttta ccattgcgat
     1861 tcctgcattg atttttgaca tggtgtttca ctcctttagc aatgatgata cataaatttt
     1921 ttgtatctag agtatagaac tttttatcga aaatcccaac taattcgtct tttcacaaaa
     1981 atgtagtcat gtttttggga atatgctaga gtagtacata gatagattca agagagggga
     2041 atggcatggt tgaatggcta ttgagacttg aaccttggca gcaagcactt gctgggacag
     2101 cttttactta ttttatgaca gctcttggtg ccgggttggt ttttttcttt aaagaaatca
     2161 aaaaagaagt gctaaacttg atgttaggat ttgcttctgg tgtcatgatt gctgcaagtt
     2221 tttggtcttt gttagatccg gctattacaa aagcagaaga aaatggagat attgcttggc
     2281 tagttgtaag tattgggttt ggtttaggcg gtgtgttttt atatatggca gataaaacac
     2341 tgccccatat gcattttgga ccacaacatg aagcagaagg attgcctacc catttaaaac
     2401 ggacgatctt gcttgttttt tctattacat tgcataacat tcctgaaggt ttagccgtgg
     2461 gggtagcatt cggtgcagca gcaactgcgg ataatccgac agccgctata ttagccgctg
     2521 tttcggtggc gctaggtatt ggtatccaaa acttcccaga aggagcagct gtatcaatcc
     2581 cgttgcgaca agaaggtctg agtcgtaaaa aggcctttgt ttacggacaa gcttctggaa
     2641 tcgttgaacc aattgctgga gtgattggag cgctactagt aacgaaagtg gagctgctgc
     2701 ttccttatgc acttgctttt gccgcaggag caatgattta tgtcgtggtg gaagaattga
     2761 tccctgaagc acagcagaca ttgtccagca aacgtcattt tgcagttttt ggcgtcatga
     2821 gtggatttat cattatgatg attttggatg tggcattagg ataaataaaa aaaatatata
     2881 tgatatatca aatagggccg cgaacttata tcgcgaccct atttttttag taagttatat
     2941 cataatgaag tgaatggtat catacattaa taatagtttt ttctatgtgt atttcctttg
     3001 atccagcgaa caagccagat gataagtgcg attgggatta gaactttgac cgcaaaccat
     3061 aaaagcccgc ttaggacact gacaaaaaga ctgatgacga agctagctgc aattagcact
     3121 aaaagaacca tgaagatatt taagaacata tttttttgac gcatacaaaa ccctcctctt
     3181 gttcattctt tactatagaa aaagtataac agggattgtt taaaaacacc tccaactgaa
     3241 gactgatttt tacttcatct acttaaatga tcaaacaatt gctttttctc tggtttatca
     3301 tctaaaatag agcggaaaag gaggagaata tcatgcctaa aaatcgtctt gaagcattta
     3361 cagatgctgt aatcgcgatt atcatgacta ttttagtttt agaacttcat gaacctaacg
     3421 gagacacgtg ggaggcgctc gctgcattag gttaccgatt ttttatctat attgtcagtt
     3481 ttatcatttt agcgatttat tggaacaatc atcatcattt atttcaagtt gtacataaga
     3541 taaatggacg ggtcttatgg gcgaacagtt tctttgtttt tgcgctttcg ctttttccgt
     3601 ttgcaacttc atggatcagt gagtacatca atagcctggc tccagaaatg acttatggtt
     3661 ttgttacctt gaccgctaat attgcgtatt atatcttggc aaaagaactt gtcaaagcaa
     3721 ataaaggcca cgagaaagca ttgaaggcga tgttgaataa ttacaagaaa tcgtatctat
     3781 cgattggttt gaatgtgtta ggaattgtct tagggtattt agtagcgcca gtagccgtgc
     3841 tgatcgcaaa catcgtgatt ctagctgctt ggatcatccc aagtcgtacc atcgaatcac
     3901 aatatcggta aaatgatccg tgtttaaaat ttaagagaaa aatatagaaa aaataaataa
     3961 acgatcaaat atccgagtga tactagtatt caaagaggga tgcagatgga tcaaaaaaga
     4021 attcatgtag gactgattac tagttttgtc acgatgctta tttatttgga tcaaggtcat
     4081 ttgacttgga cagtagttgg tgtcgtagga tttttgacat tattcacagc aataaaaaaa
     4141 taaacatctt tatgtaatct gatgttctat ctatttaaga aaagcatgtt tacagcagta
     4201 tgattctgtt gtaaacgtgt ttttatttgt ggaaaattta atttttagaa aagtgtatcg
     4261 aaaaatagat agttagtttt tccacagtgg ataacttcgt aagagataaa aataaaaaat
     4321 tagtcaaaga agggcgtata gattt
//
LOCUS       DACENJ010000191         4305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.197, whole genome shotgun sequence.
ACCESSION   DACENJ010000191 DACENJ010000000
VERSION     DACENJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.197"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            148..1137
                     /locus_tag="IW748_002279"
     CDS             148..1137
                     /locus_tag="IW748_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1105178.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="IW748_002280"
     CDS             1134..1553
                     /locus_tag="IW748_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105179.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="IW748_002281"
     CDS             1562..1870
                     /locus_tag="IW748_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105180.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="IW748_002282"
     CDS             1874..2236
                     /locus_tag="IW748_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105181.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="IW748_002283"
     CDS             2458..3864
                     /locus_tag="IW748_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1105182.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1497 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttta
     4021 aagggtgcgg ttttatgttt gcatgactaa gaatgagtgg gaaaatttaa cagccctatc
     4081 taacaggcgg ttaaatgtac aaaatatgta caccccctta aaaatgcagt ggtcgattga
     4141 tcgaatttta tgtctttggg ttttggggtt ttaaaacgaa aaaaaggggg cgattttcgc
     4201 cctctttttt gatgtttttt cttatcttga tactataggc aactatttct gcgaagaaat
     4261 cggggctata gtatgggtct atcccttgtg gctctaagga agaac
//
LOCUS       DACENJ010000192         4261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.198, whole genome shotgun sequence.
ACCESSION   DACENJ010000192 DACENJ010000000
VERSION     DACENJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.198"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1222)
                     /locus_tag="IW748_002284"
     CDS             complement(<1..1222)
                     /locus_tag="IW748_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1105183.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(1253..2092)
                     /locus_tag="IW748_002285"
     CDS             complement(1253..2092)
                     /locus_tag="IW748_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1105184.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(2109..2789)
                     /locus_tag="IW748_002286"
     CDS             complement(2109..2789)
                     /locus_tag="IW748_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1105185.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(2791..3864)
                     /locus_tag="IW748_002287"
     CDS             complement(2791..3864)
                     /locus_tag="IW748_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1105186.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
BASE COUNT         1166 a          947 c          739 g         1409 t
ORIGIN      
        1 agaaagtagc gccctcttgt ttttttcttg ctactatttc ttgatgttct tgcttgattc
       61 tttctaatag ctttggctct ttcaacagat caagagctgt attggcgagt agttctgctg
      121 caatcgcaat agaagcaagg cctttgtcac ttttagcagc agctttgaat tcttctgtgt
      181 gtccggcaat atatgtctca gagattgcta ccgtaggctg gattgttggt atgacttggc
      241 tgacatttcc tacatcagaa gaaccgagac ttgttctttg ttccgtttct atttcttcat
      301 ctggaacacc tgcttctttt gcatgctgga agaataagtc atcaaaagaa ggagtaacga
      361 tgacatcatc aactgcattt tggaacaatc caaactcaaa agtcgtgccc gtacttatcg
      421 cagcacctcg tacgatattt tccacttttt catatacttt atctaatgtt tgccgatttc
      481 ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat gacgttggca gctactcctc
      541 cgtctgtaat gatcccatgg atattgacat cttttggcaa ttgcaagcgt agagcgttga
      601 ttccattaaa cacttggatc aatgcttcca aagcatttac tcctttttca ggtgctgctg
      661 ctgcatggga agcgacaccg taaaatttga tgtcgactgg atcattcgct aaagattctg
      721 tcgttttccc ataacgaaaa gctggatgga cgcataacgc tacatcgaca tcctcaaaaa
      781 aaccttctcg aacaaagctg cctttagctg atccgttctc gcccccttct tcccctgggg
      841 tgccatatac acggatttct ccaccagttt catcaatgaa ttgcttcact atgctggcgg
      901 ctaatacgga gtaagtacca aacaaattat gcccgcatgc atgaccaata cctggtaaag
      961 catcgaattc tgccaaaaag gcaattgtgg gaccgggttt attggaaata taacgtgcat
     1021 caaaggctgt tcggtgtcca gctacttctt tttctaccca aaacctttct ttttctaatt
     1081 gtttaatcaa cgctgctgag gaataaaact catagttact tacttcagga tgttcgtgga
     1141 tatctagagc aagtgcttga tattctggta aacgatattg gattagatcc ttcaattggt
     1201 ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc tagtcatttt tcttattttc
     1261 tcccgaatgt ttcccatgca ggcacgcttg atccttttga tgttttctcg attactttga
     1321 ctgtgtcttc tgtttgataa gcgtctacta ttttttgata cgttttattg ttttcatctt
     1381 cttttcttgc aacgatgata ttcacataag gacgtgcatg gtcatccact ggttctaaga
     1441 agatggcatc agaagcagga ttaaatccag catctacagc tacaccgcta ttgatcactg
     1501 aaatgtctac atctgacaaa gcacgtgctg tttgggaagc atctaattca gaaatgtcta
     1561 gattttttct attctcggta atatcagaaa cagtgggcgc ttgtcctttt gccttatcca
     1621 ctttgatcaa tccagcagtt tgcaataaca gaagagctct gccgccattt gtaacatcat
     1681 tagggattgc tacttttgct ccgtctttga tatcagctac atccttcaaa gtgttagaat
     1741 aaattcccag cggtgcattg acagtatttc cgatcgatac taaatcagtt ccgtgttctt
     1801 tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt caaatcaatc gatccatctg
     1861 ctaaagccac atttggttga ttgtaatctg taaatttcac gtattccaaa ttaatgttct
     1921 cctttttcaa acggtctttc acgttgtccc atacatctgt atcttctcca ataatcccta
     1981 gttttacgat agtttctttt ttgtcagttg tatctgcttt tccttggcta caccctgcca
     2041 accaaataat tgctacaaat gtaatgacta caaaacgaat aatttttttc attttttctc
     2101 ctccactttc aatgtgtaat ttttttaatc agttgattac tgataaattg acttaaaaaa
     2161 actaagatta gaatcaataa tgtagccaca atcgttacat ctgtttggaa tcgattatac
     2221 cctctcgata tagcaagatt ccctaatccg cctgcaccta ctgctccagc catggtagtc
     2281 agtccgatca cgttgatgat agtcaacgcc gagacccgaa tgataccagg caaaccttct
     2341 aacaaataca cacgtaaaat gataccgatt ggactggttc ccatcgcttc tgccgcttcg
     2401 atcaccccag gatcaacttc tagcagtgcg ttctcgattt gacgagcaaa aaacggaaca
     2461 actccaaaaa tcaatggcac taaagctgct ttcgcaccaa tcgtcgttcc aactaagaaa
     2521 cgagtagtca atgctaaaag agaaagcaag ataatgaaag ggatggaacg aatgatattg
     2581 atcagcttat caagtatttc aaatagcaga cggttagcta gtaatccatt agggcgtgtc
     2641 actactaacg tcacacctaa gatgattcca atcatcccgc caatgaatgc tgtccaaaaa
     2701 gacatatata gtgtttgctt cgtcgcttcg acaaattcat ccggaatcaa ataaacattg
     2761 ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct ccttcgatca ctttgatacg
     2821 actatctaaa tcgttttttt gtaaaatatt tatttttaca ccagatttga tcaaataatc
     2881 caatgctttt ttccgctgtt tggtttctcc agctaaagta acgatcaaac tccctaacgg
     2941 cgtttcttgt aataactcta cattgccata taaaatattc gctgttactt gatatttgct
     3001 gtacagatga gcgattagag gttcgttcgt ctgatttcca atataactta attccaccag
     3061 ccatttattg gcgatctgat cggcaaaagc agaatgggca atgatcgttt ctaatgcttg
     3121 gtcaagatga gtagctgttc gaataaaatc tttggtcaat tcttcttcag gattgctgaa
     3181 gatctggatg ctgctacctt tttcaacgat tcttccatct tccataaccg ctaccttgtt
     3241 gcagatttct ttgacaactt gcatctcatg agtgatcagg acgattgtta aacctaactg
     3301 gcgattcaat ttttttaaca atgcaaggat ctgaagggtc gttttcggat caagtgcact
     3361 ggttgcttca tcacataata ataccttagg ttcattcgcc aaagctcgtg cgattgctac
     3421 tcgttgtttt tgtccccccg acagttgttt aggataagca gatgcttttt cttccaaacc
     3481 gacaagttcc agtagttcat tcactttttg acgacgttcc tgtttcgatt ttccagaata
     3541 ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt tccattagat tgaaatgctg
     3601 aaagatcata ccgatggcct ttctttcttt tcgcaattct ttgggagaga gctgactcag
     3661 attcgtctga ttgacaatga cttctcctgc tgttggtttc tgcagcaaat tcatcacacg
     3721 aactaacgta cttttccctg cacctgagta accgacgatt ccataaacat cgcctttttc
     3781 aatcgttaaa tctacagaat ctactgcctg tatctctttt tctttttgtt tgaaacatac
     3841 agaaacatct cgcaattcaa tcattaaaat tcctccttat ctaaaaaaag cgtcctcaag
     3901 aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt gttaccacct catgttcaag
     3961 ctgtgttcac acacaactcc tcttcaagta cgatcatact ctagcaagat atcggtcgca
     4021 gccgtcatcg ccttacatct atgctcggga tgaattgctc gatggccatt ttccaatagt
     4081 cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa aggttcttct atcgtactta
     4141 ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt actaacataa actatgtcag
     4201 taaaaggacg acgtatcgcg gtaccacctt ttttcggaag tttccttccc tcaagcccaa
     4261 c
//
LOCUS       DACENJ010000193         4242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.199, whole genome shotgun sequence.
ACCESSION   DACENJ010000193 DACENJ010000000
VERSION     DACENJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.199"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(67..597)
                     /locus_tag="IW748_002288"
     CDS             complement(67..597)
                     /locus_tag="IW748_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1105187.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            702..1253
                     /locus_tag="IW748_002289"
     CDS             702..1253
                     /locus_tag="IW748_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1105188.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1406..1879)
                     /locus_tag="IW748_002290"
     CDS             complement(1406..1879)
                     /locus_tag="IW748_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1105189.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2017..3489
                     /locus_tag="IW748_002291"
     CDS             2017..3489
                     /locus_tag="IW748_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1105190.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3486..4196
                     /locus_tag="IW748_002292"
     CDS             3486..4196
                     /locus_tag="IW748_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1105191.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1326 a          762 c          825 g         1329 t
ORIGIN      
        1 atttttccgt ttctttctct aatactcagg tcagaacgct gatttcacaa cctctctttc
       61 acaaccttat tttactcgtt tataattttc atcgaagtat tttccgctac ggatatcatc
      121 tggcattcca tcgtatggtt cttcgctaat aacatcttca ttgtctatac ctgcatcacc
      181 catataagtg atcgtagcaa attcaggaac aagtaatttt ggataagttt catatttttc
      241 acgagctgct tccatttctg cgatatgttc gttttggaaa gtaacagtga tttctggatg
      301 ttcttcaacc catttaggga aaaagatcat tccgtgaagt ttcttttcat ttggcataaa
      361 gtccaatgcc acgtctgtcc ctgttggctc tgtccaagtg attttgaata cgccgtccgt
      421 caatttaacg atatcagctt cttggtcttt tacccaacgg cctttgacca ttccgccatg
      481 gatacggtaa tcaacagtat ggtcattttt ggcataccat tcatattccc aaccattgtc
      541 atacgtatag ataaagtgag ttccaataaa atcttccaat tctttaaatt ctttcatgtt
      601 taagcatctc cttataaatt aaaacatgta ataaactaca tgttttcgat tactcattta
      661 tgatagaatg gatttaaaga tttgtcaagg agggaaaact gatggcaaga aaaaatacac
      721 ttcgatatat tttgttaggg ttattaagta agaagaagat gacaggatat gaattgaatc
      781 aatcgttcaa aaatgaaata ggagaatttt ggcaagcaaa acatagtcag atttatcctg
      841 aacttgcaaa aatggaagag caaggaatca ttcagcatca agtagagatc acaggtgaaa
      901 aattggaaaa gaaagtttat gaattaacag aagaagggaa ggaacaattg gcagagtgga
      961 ttcatacacc caccaatgaa ctaccggtta atcgagacga atttgttctg aaattgtatt
     1021 tcgtcaaaga cgtgaatgat ccggcattag tagaaattat tcagcagcag cgtgaccttc
     1081 atgaagaaaa acgacttcat ctattagagc gaaaaaaaac gatctttcct actgaaaaag
     1141 aaaaacatga aaattacgga cactacctga ttttagcgca tgcgatcaat cgggaaacag
     1201 aatatgcaaa ttggctaaca gatgtattag aagaaagtaa ccgaaaaaaa taaaaaaata
     1261 tccgaacagt gatggagaat tcctttctat atgaaagaaa cgcagatcac cgttcggata
     1321 ttcttatgtt caaacaggcc ttttttcaag tatttatgta gcatctactt attaaaaagc
     1381 ttggtactta ggagattagt ggcgtttaga gttcttttaa caactgttcg tagtcagctt
     1441 tttcatcgac aggaaggagc ttgtaagtta attcgtctgt ctcgttgtca tgaatgatgt
     1501 acatcttagc attctcttct ttgaagttac cgatttgtaa aatcgtatag cgttcgtcga
     1561 tcaattcgat atctttgatt gcttttgaaa aaactttacg gacatggtgc attttctttt
     1621 ggcggatttc tgccttttct gcagcttttg acaaagtaaa tccgtcatct aaatattttt
     1681 tgatcagttc tacttttatg actgtaggta aacgatattt tctaggacaa ttcgcatcag
     1741 cttcgatcga acgaatgaat cccttttgtt cccaatacct taattgacgt ggagacacac
     1801 ctacgatatc acttaattca ctgatgccaa caaccaattg atcattttcc aacaaacgtt
     1861 taagggaagc cccaaccatt tttgtcactc cttttgtcct cttgcttccc ttaattatat
     1921 ccgtattaac ttatgtgtca agtaggttga caaatagcga atttaggact aaagtattct
     1981 agtagtttca gaaaaaattc agaaagaagg agaaatatga gtaacaacca acccgtagat
     2041 atttacggaa aaccatataa tcggacactt ttagttgttg tcttattaat tggtacattt
     2101 tgtaccgttt tgaatcagac cttattgacg acagcttttc ctacattgat gaaagctttc
     2161 gatatttcag cgtctgatgt gcaatggtta acgactggtt tcttgctagt caacgggatc
     2221 atgatcccaa taacagcatg gctgatcaat aaattcagtt caagaaattt atatctttct
     2281 gccatgacga tctttttgat cggaacgatt acttgcttta cagcacctaa ttttagtaca
     2341 ttactaatcg gtcgtttgat ccaagcttgc ggtgtgggag tatcgatgcc attattgcaa
     2401 aatatcatgc tatctatttt cccgccagaa aaaagaggat cagcaatggg gatgtcaggg
     2461 atcgttattg gacttgctcc tgctttaggc cctacattat ctggttatat cattgataac
     2521 taccattggc gtgacttgtt tggtatggta ctgccaatcg ttgttcttgt cttggtttta
     2581 gcattcttcc taatgaaaag tgttattcca ttgtcaaatc ctagcattga tattttatcc
     2641 gctattttat caacgattgg attcggtagt ttactttacg gattttctag tgtaggagat
     2701 gacggctggg gaagcttgaa agtaattggt ttcttagttg ttggtgtgat tttcatcgcc
     2761 ttgttctcat ggcgtcaatt acatttggaa catccattct tggaacttcg tgtattcaaa
     2821 tcatctacat tcacgattgc agctgtttta gctggtgtaa caaatatggc gatggtaggg
     2881 gcagaaatgg tcgtaccatt gtatattcaa aatattcgtg gagaatcagc attccattct
     2941 ggcttgatgt tgttgccggg tgcattgatc atgggggcta tgatgcctgt gacgggtgcg
     3001 attttcgata aatacggcgc gaaacgttta gccatcacag ggatgttcat tttgacagcg
     3061 gcaactgcac catttgcttt cttgacaaaa acgactccag ttttatatat cgtgatcctt
     3121 tatgcgatcc gtatgtttgg gatctccatg gtcatgatgc cggtaacgac ttctggtatg
     3181 aatgcattgc caaataactt gatcagtcat gggacagcgg taaataatac attccgtcaa
     3241 atcgcaagct cgattggtac agctattttg atcagtgtgt tgacgaatgt gacaaaagca
     3301 aatatcccaa gcaaatctgt attggataat acgccattag cttataaaga tcaggcaatc
     3361 aatgccacac tgtcaggtta ccatgccgcg tttatcgtag cgatcatctt tgggattctt
     3421 ggctttatta ttgccttttt attgaagaaa aaagaacctg ttctaatgaa agcgggggat
     3481 aatgcatgat tattggagtt ttgattgtca gcttgcttgc ttttgcttta acgaacgtgt
     3541 tcgctaaaaa aacatggcaa acctttttat ctttgatttt cggactaatc ttcgtcgctt
     3601 cactaagttt gatcgtcgct aatttgtcca atcactttgg aatggaaaaa gtaacggaaa
     3661 caaaaacaga aaaaattgtt tccagtgctg attcccaagg agcagatatg cttttgtata
     3721 aagctttagg gaatggaaaa gaaaaagtgt atctttaccg aacaaatgaa aaacaagaaa
     3781 aaccaaaagc aactggtacg gataacgaaa caaataaagt agaaaagaca gacggcgatg
     3841 ctgaaaaagt gacgaaaact acttattggg aatacaaaaa cgacatgtat aaattctggt
     3901 tcaatattgc tgataacaat cacgaatatg acagccgagt gaatacattc aaaatcccag
     3961 agacatgggt agaattaagt acggaccaag cagccaagtt agctgaatta gtgaagaaac
     4021 agcaaagtac gatggaaagt gaagcaaaag cttatgttca agacggtatg gtaaaagcaa
     4081 tgactgaaaa tccaaaaatg agcaaagcag agcaagaaca acgtacgaaa gccttagctg
     4141 ctgaattcca acaacaagca ttcgcaaaat tagtgaaaga agcaaaaggc gaataatatt
     4201 ttgaacacca tgtaggaggt tgtgaaaaag ctgctctgca tc
//
LOCUS       DACENJ010000194         4182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.200, whole genome shotgun sequence.
ACCESSION   DACENJ010000194 DACENJ010000000
VERSION     DACENJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.200"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1358
                     /locus_tag="IW748_002293"
     CDS             <1..1358
                     /locus_tag="IW748_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1105192.1"
                     /translation="DSYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPL
                     KGKVVLGFDPAYRTGCKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKY
                     QVDMVAIGNGTASRESELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQ
                     VEERSAVSIARRLQDPLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVG
                     VDVNTASPQLLQHISGLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGF
                     LRVPGGKNILDNTGIHPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESL
                     SVGEETLADILAGLTQPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGA
                     FVDIGVKQDGLVHISKLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYE
                     E"
     gene            1378..1860
                     /locus_tag="IW748_002294"
     CDS             1378..1860
                     /locus_tag="IW748_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1105193.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            1876..2637
                     /locus_tag="IW748_002295"
     CDS             1876..2637
                     /locus_tag="IW748_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1105194.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            2768..4102
                     /locus_tag="IW748_002296"
     CDS             2768..4102
                     /locus_tag="IW748_002296"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1105195.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT         1403 a          752 c          911 g         1116 t
ORIGIN      
        1 aggatagtta taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga
       61 ctgaaaaggc agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt
      121 tgcaaccgcc actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat
      181 gtaaactagc agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc
      241 ataaacctgc agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta
      301 ataaatatca agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac
      361 tttttgtggc tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg
      421 aagcaggcgc ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac
      481 aagttgaaga aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat
      541 tagtcaaaat cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa
      601 aacgtttggc tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg
      661 atgtgaatac agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg
      721 cccaaaacat tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga
      781 aaaaagtgcc acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc
      841 ctggtgggaa aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa
      901 aagaaatatt aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag
      961 aaaaattgac acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa
     1021 cgttagccga tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc
     1081 cagcgccctt attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat
     1141 tgactggaac ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc
     1201 aagacggact tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg
     1261 tcgtgtcagt aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggca
     1321 gaatcagttt gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg
     1381 acacaagaag aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct
     1441 ttcaagcacc aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta
     1501 aacgatcatc atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt
     1561 gcaggaatca tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat
     1621 cagcataaag atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca
     1681 ccagccttga cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc
     1741 acgatctgcc gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga
     1801 gaaatcaaat ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga
     1861 aaggatagaa ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta
     1921 ttaaattttt tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt
     1981 attttagctg gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat
     2041 ggccaagtag atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat
     2101 gagaattttg aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt
     2161 cgtcagtatc taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag
     2221 tctacaaatt ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct
     2281 gtaccagaaa aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact
     2341 tttgagaaag tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg
     2401 gaaatccttc acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg
     2461 cctaaggaat atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag
     2521 aacggctatg gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt
     2581 tggtcttctt acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca
     2641 ttttagcaag cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa
     2701 gataatgagg agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag
     2761 gattcaaatg gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat
     2821 gagctccttg gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga
     2881 aaaatatttc tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa
     2941 cgcagataat gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca
     3001 tgaagaaatc cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat
     3061 tggtcatttt atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag
     3121 tacgactgga ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg
     3181 agatggaaca ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata
     3241 tcgccgtcat ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga
     3301 tcatccggat tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg
     3361 acaagtgaaa aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc
     3421 aaatgtgccg atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat
     3481 cgaacgcaca acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca
     3541 cttcactgta ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt
     3601 agcttatttc gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg
     3661 agtaaaacgt cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc
     3721 gcatcaccct gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa
     3781 agaaataatt gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga
     3841 atttgcagaa gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc
     3901 tcgggaagaa caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg
     3961 agaagtcatc aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat
     4021 cttcatgggt gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag
     4081 cacgacgaaa aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg
     4141 gggagtggca aaaatcttgc caagccccct tttttgcaac tt
//
LOCUS       DACENJ010000195         4074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.201, whole genome shotgun sequence.
ACCESSION   DACENJ010000195 DACENJ010000000
VERSION     DACENJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.201"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..1051)
                     /locus_tag="IW748_002297"
     CDS             complement(77..1051)
                     /locus_tag="IW748_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1105196.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2915)
                     /locus_tag="IW748_002298"
     CDS             complement(1500..2915)
                     /locus_tag="IW748_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1105197.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESISAEMRYISKQREK
                     NEREKALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLNSFEKLSDGSYRLSSADF
                     VDLYQKASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDVSA
                     VVAENKKLKEENRSLRRRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKT
                     LASQQNAPERNIGRSENEMEW"
     gene            complement(3137..3499)
                     /locus_tag="IW748_002299"
     CDS             complement(3137..3499)
                     /locus_tag="IW748_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105198.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVSLR"
     gene            complement(3503..3820)
                     /locus_tag="IW748_002300"
     CDS             complement(3503..3820)
                     /locus_tag="IW748_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105199.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQIGVIYRNKSKG
                     KEL"
     gene            complement(3921..4061)
                     /locus_tag="IW748_002301"
     CDS             complement(3921..4061)
                     /locus_tag="IW748_002301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105200.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYIYAVAESSGEA
                     ND"
BASE COUNT         1153 a          896 c          576 g         1449 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attctgcaaa ggtttctcgt aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca gcaaatttcg atatttatat tttgtctttc
      241 gattaatttg cccccaataa tcatgttctg ttgttaaagc agtaatcatc attttgtcag
      301 ctacacttgt atcagcaggc aagtaatgag gactagcaaa actatctaag ctttcataaa
      361 ccatagcatg ccaatcagtc gctttccctc gtcttaattg taattctaac cgccaccaac
      421 tcacgatttc ttttggcaca atttttcttt tcttctcttg ttccagtttt ttattataca
      481 tccgaatttg acgttcagaa gagcgagatc cccaatatgc cgtctctaat tttccattcc
      541 gtccataaat aggcttaaaa gaaactggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ctgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaaatttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccgttacctt
      841 catttaggcg cataagctgt tcaaaatcta aaatcaacac atcatgtgta gtgtgataat
      901 agatattctc tttaagattt ccaacaatgg taattcggtc gattgaccac tgaattttta
      961 ggggggtgtc cataaaaggg acacttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aatttagttc ctcctcaaag aaaaaagaga
     1081 ttggtggaaa gaattttcca gagttcacgt aactctaaaa aattcataac cagcaatctc
     1141 tagtcttgaa gaaacctgaa attattcgta taatgaattt gagttacgga gctagtgtga
     1201 ttagtttccg taaaggtcga gaacgtgtcc agcgttgctc gactttattt ttttgctctt
     1261 ttttcagttt atttatttga taactcaata atagcacttc tctttagcta tgcaaatatc
     1321 caaaaacata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaagtt
     1381 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacagaccg cctccggtac cgttgactgc accgtataac
     1501 taccactcca tctcattctc agaacgccct atattacgtt ctggggcatt ctgctgacta
     1561 gccaacgttt tctcttgatc taatattttt agctcttcag atattcccat atctttttct
     1621 acaagcccaa aacgaattga taaacgagtg attgattttt gaaatgcgtt tttcagttga
     1681 gttaaccgcc gacgcaaaga acgattttct tccttcagct ttttgttttc agcaacaacc
     1741 gctgacacat caaccttttg ttgatttttc aaaaaagaat tttcttgttc taacgccatg
     1801 tttttatcaa ataaagcgat atttttatct aatgtatttc caagcgattc attcgccata
     1861 tcagcatatt ttttgctgtt cgtagcatta gacgcttttt gatacaaatc tacaaaatca
     1921 gcactagata agcgataaga accatctgat aatttttcaa aactattgag tggcacagga
     1981 tctttttgaa atacaacagc cagctctttt tccaaatcga taatcgattt tttctcttct
     2041 tcaattttcg ccttctcaga agccaccaga gccttctcac gctcgttttt ttctctttgc
     2101 ttagaaatat acctcatttc cgcagaaatg ctctcacgct cttttacagc cgtttggcgc
     2161 aattcagaaa tttcactcat gatttctttg tattcgtgaa tatctttaat tttgttggta
     2221 ccgacaatat cccttttcca accgtaatca ttcattaatc gttctataga gtcaacttct
     2281 tgatttctaa aattattgaa aagcactcga ccattatctt tatcagcgtg tatcccttgt
     2341 tgaaccagtg ctttattaaa gctcggctgc ttttgaacac cccgtttata accttcagca
     2401 accggcacaa tattcaagtg caggtgcgga cttatttcgt cattatgaat aacagcatta
     2461 taaacagcca aattagggtt tctctcttga aactccttgt aatactgaac caaaatttta
     2521 ttggcaatca ctcgattttg ttcagcttgt tgtttcgata tgccagtaga agaaccatct
     2581 gctcgatttg tctggaaatc atctaaaatg cctatttgca caataaattc tcgttgaggt
     2641 tccaactttt tatccttgcg cactttcgct aaatagtcct cgatttttct atcggctctt
     2701 ttttgtttcg cattatattt ttctaccact tcgccaaaca attcctcata tttatctgct
     2761 agattcgtct gctctaaata aatattttca tccgatttcg tgaagtcaat atgcttatgc
     2821 caatcattgg ttttctggct atcagttaac tcacgattat tatgagaaat acttgtattc
     2881 ttcgttcctt ttttaaatga aatactaaac cccaagttaa aaccctcctt cgcgctgtcg
     2941 ctaataaaaa gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat
     3001 tgtgacatgg ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca
     3061 acgaaaaaag gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt
     3121 cacgcaaaat aatcgcttat cgtaaactca cgtcattaaa cattgacgta aatttactct
     3181 cactcatctg acttttttta ctcggtatca ctccgagtaa gccgtcagat tcattgggat
     3241 aatctcgttc gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt
     3301 cgtgttgctg atcgtcagaa attgacgata accaccgctc aaaatcatca aaagattcaa
     3361 atccaaaatg cttccgaagt gccttaccca tttcagcatt ttccttctca atcaatcgtt
     3421 ttttctgttg acgaagtttt gcctgttctt ttttcaattt ttcttcttgt gctttaatat
     3481 ctgctaaagt cattcccaag atttacagct ccttgccttt tgatttattt cgataaataa
     3541 caccaatttg atttaattgc ataacataag ttaaaatatt cagccaaaat tccttttcag
     3601 attcatcttc tgtttcggct attttaccgt aaacaaattt atcagtaatc aaagaagaat
     3661 ttgctaggcc tacattgatt gtagaattga gaacgtccat cattctctca agcatttcac
     3721 tataattatc ttcatccggc aaattcaaat aatcagctaa tcgttcatac atcaattcaa
     3781 ttctatcaac tgtctcttct cttaaatcaa ttttatccat gttacacctc atttccaaaa
     3841 ctgccaccat ttccgattct ttaattttcg atacacctaa ttcatcagca agatttttta
     3901 tggtcatctt ttctgacaaa tcagtcatta gcttctccac tactttcagc taccgcataa
     3961 atatatttaa cggcgtgctt agggataata atcggagagt caattttatt atttttcaaa
     4021 atcaattcat attgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACENJ010000196         4045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.202, whole genome shotgun sequence.
ACCESSION   DACENJ010000196 DACENJ010000000
VERSION     DACENJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.202"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..709
                     /locus_tag="IW748_002302"
     CDS             <1..709
                     /locus_tag="IW748_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1105201.1"
                     /translation="DNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGI
                     IPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVL
                     KVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLR
                     ESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGV
                     SPKQYQLGLVDNFNNIS"
     gene            726..1160
                     /locus_tag="IW748_002303"
     CDS             726..1160
                     /locus_tag="IW748_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105202.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1777..>4045
                     /gene="esp"
                     /locus_tag="IW748_002304"
     CDS             1777..>4045
                     /gene="esp"
                     /locus_tag="IW748_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1105203.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1483 a          555 c          768 g         1239 t
ORIGIN      
        1 agataatctg ttatcacaaa attacgatcc agaaatatat ctgtttcttt atgaaaataa
       61 aatactggaa tatgttaaaa atggagatat tcgaaattta gaaaacatgg tttttaatct
      121 gagtaatgga ataattccaa gtgttagtgg agatacgatt cggtctgaaa aaaattattc
      181 tatcattgtt tttgaaaagt tagcacaaac gtctataaca ttaggaatgg atataataga
      241 agcgtatcaa agtagagatg ctttgataca agaaaacgaa ttagctgtat cgttacctga
      301 ggtcttaaaa gtgagggatt ctgggattgt ttattatact aaggaaatcg gaaaaactaa
      361 gatagagcat ctttctccct taatttcttc tgtcgttcaa ttcatcggct taaatatcta
      421 taaaagaatt actgtaaagg aaatagctaa ttatttttct gtaagtgaaa ctaaattaag
      481 agaaagcttt aagaatgaaa tgcatattac gatttataat tatatctcta agcgaaaaat
      541 ttctacggct aaaataatgt tgaaatcaaa ccatactatc agtgaagttt ctttaggttt
      601 aggattttca gattcttcac acttttcgag agtattcaaa aagtacgcag gtgtatctcc
      661 taagcaatat caattaggtt tagtggacaa tttcaacaac attagctgag aggttttatt
      721 ttattatgag actatatcat ttacgtagtt tggaggagtt gaggaaattg gattttaaca
      781 aaaaattttt ttgggaaatt tctgatttga gttgtttcgt agaatctgag cgtctaagta
      841 atggtaataa tagtgtgtta aatgaatatg acaaagtagt aattaataaa gtaaaaagtt
      901 tatgtgaaga agctagagaa tctatcagtc atattaatgg tagcaatttt aaacaaacat
      961 tatttaatct gattaagcta gatgctaaaa tatcaagtta tttattcttt ttgatacatg
     1021 atgaatttat ggattatcaa aaattggatc agttaattga aagagaaagc tgggaagatt
     1081 attacgtggg tctaactttt tcagaaaagt taaacaacta caagttaata gattataagt
     1141 atttgagtga aagcaattaa ttattcatgt agagtactag acactttaaa tatggacaat
     1201 tcaattataa agaaaaagaa tcaataattt aatgaggctg tgacaaatgt cattttttgt
     1261 ttcgctaaaa acgagtgaaa aaatgttgat aactccaatc attcgtttaa gattctagga
     1321 atcattcaga aatgctgtga aaccgatact ttataattat aaagaaggtg atttcaaatt
     1381 ccgaagaagg aatttgaagt caccttttta cttatgtcac agtctcattc agtaatgcgg
     1441 gataaggctc cttattccgt tatttgaata ctctgaagtt taatctaaga aagggtaata
     1501 ttttgaaaac atgcttcatc gcatagaaat gaacttaatt tcagaaagaa tacatacata
     1561 tctttttata tgttttcagc tgctgaatgg ttaaaaagca caaatatccg aaaaaaacat
     1621 gaagaaatgt actttttttg ttgtaatgtt catgaaattc tctaagtaaa ttagttaaca
     1681 taaggttagt aagattccta ttctgtgaat aatacgtatc agatttatct tttattctta
     1741 ctaggaaagt aattattaag ggagttgatt tgaataatgg ttagcaagaa taataagaga
     1801 gtatttctag aaaaaacaaa gaaacgagta ttgaagtaca gtatcaaaaa attaagtgtt
     1861 ggggttgcat ctgttttgat tggtgttggt cttgtcttgg gaacaactga actagttaaa
     1921 gcacaagatg aaatttctcc aagcagccca cttgaaacag cgataagctc cgtacaagta
     1981 ggtgacaaag tagcttcggg gaatactttt caggagaatc ctggttatac gaaaaattat
     2041 aacttttcgg atttacaatt tagtcctcaa gaactaactg gagacacact aaaaggcaat
     2101 acaattggat ttgaagttta cggaaaacat aatattgcag catcaactaa aaactgggaa
     2161 atccgtcttc aattagatga acgattagct aaatatgtag aaaaaattca ggtcgatcca
     2221 aagaaaggaa ttggatcttc tagacggacc tttgtaagaa ttaatgattc gctgggtaga
     2281 cctacaaata tttggaaggt caattatatt cgtgcaagtg atggattatt tgctggggca
     2341 gaaacaaccg atacgcaaac cgctcctaat ggggtaatta cgtttgaaaa gagcctagat
     2401 gaaattttta aagagatagg catagacaat ctcaagaccg atcgtttaat gtatcgaatc
     2461 tacttggtaa gtcatcaaga cgatgataaa attgtaccgg gtattgatag tacgggctac
     2521 tttttaacag attcagatga tttctataat agcttagatg tatctgaaaa taatcctgac
     2581 cagtttaaac atggatcagt aagtgcaaaa tatgagaaac ctaatactca aacaaaagat
     2641 ggttcaggat ccacaggtgc taatggagct ataatcttag atcataagtt aacgaaaaat
     2701 tataatttct catattcatc ttccgcaaag ggcacgcctt ggtatgctaa ttacaaaatt
     2761 gatgaacgat tagttccata tgtagcgggt atacagatgc acatggttca ggctgacaaa
     2821 gtaacatatg atgtttcgtt tgaatccgga acaaaagtag ctgatttagc aatcgagcga
     2881 cgggaaggtc atgaaaatta tggcataggt tcaataacta ataatgattt aactaaactt
     2941 atcgattttg ctaatgcaag tccacgtcca gtcgttattc gatatgtttt acaattgact
     3001 aagccattag atgaaatctt agaagatatg aaagcaacag cacaagttga agaaaataaa
     3061 ccatttggtg aagatttcat ctttgattct tggttgtcgg atacgaataa aaaattaatc
     3121 cagaacactt atggaacagg ttattattat ttgcaagata ttgatggtga tggaaaccct
     3181 gacgataaag aagagagcgg agacacgaat ccatatatcg ggaaacctga attagaagaa
     3241 gtatatgatg ttgacacaac agttaagggg aaagtattca tccacgagtt agcgggaaca
     3301 ggtcacaaag cccaacttgt tgataaagaa ggtactgtat tagcagaaaa aactatcgct
     3361 ccaaatgaaa aagatggggc tccaatttca gatactgtag aatttgaatt tacgggtgta
     3421 gattcaagta aattaatcgc gaaagatgaa ttaaaaatcc aaatcgtttc tccaggtttt
     3481 gataaaccag aagaaggttc aaccgttatt aaggaatcac caaaagcggt tgataaacaa
     3541 accgtggtag ttggatttaa accagatgct aaagaatcaa ttcggaataa taaaaactta
     3601 cctgaagatg cagagtattc atggaaaaca gagcctgata cttctaacgt tactgatagt
     3661 acgaaaggta ttgtaactgt taagatcgga aatcgaactt tcgacgtgga tgtagagttt
     3721 gctgtaaaag cttctcaagc tatggaaaat gatgcaacat acgtacctat aacaacaact
     3781 ccagaaacga cagttcaaag tggaaaacct acatttgata aaccagatgt tcctttagct
     3841 aaagatgcct tttcaatttc agatgtctat aataaggact tcggtaatgc aagtgttgat
     3901 gcaaacactg gtattgttac attcactcca gctaaaggtg taggagaatc ggagccgatt
     3961 actggaacaa ttcctattaa aattgtttac caagatggtt ctgtaggcac gaccgattta
     4021 gcagtaactg taagtaaaga tattt
//
LOCUS       DACENJ010000197         4018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.203, whole genome shotgun sequence.
ACCESSION   DACENJ010000197 DACENJ010000000
VERSION     DACENJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.203"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            118..1005
                     /gene="xerD"
                     /locus_tag="IW748_002305"
     CDS             118..1005
                     /gene="xerD"
                     /locus_tag="IW748_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1105204.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            1024..1395
                     /locus_tag="IW748_002306"
     CDS             1024..1395
                     /locus_tag="IW748_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1105205.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            1365..2162
                     /locus_tag="IW748_002307"
     CDS             1365..2162
                     /locus_tag="IW748_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1105206.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMVIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            2146..2715
                     /gene="scpB"
                     /locus_tag="IW748_002308"
     CDS             2146..2715
                     /gene="scpB"
                     /locus_tag="IW748_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1105207.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            2721..3437
                     /locus_tag="IW748_002309"
     CDS             2721..3437
                     /locus_tag="IW748_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1105208.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      3519..3644
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3767..>4018
                     /locus_tag="IW748_002310"
     CDS             3767..>4018
                     /locus_tag="IW748_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1105209.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTA"
BASE COUNT         1355 a          700 c          902 g         1061 t
ORIGIN      
        1 aaaaagaaac cagtccgtct gagtgtctag acggcttttt ctttgctcag attatggaaa
       61 aagactatca gaatgaagcg gtatctgata tgatagagag gcatgagggg gattattatg
      121 aaagagcaaa tcaacgatta tctccactac ttgaatattg aacggggatt gtccgccaat
      181 acacgacaaa gctatgaacg tgatttggaa cagtacttgc attttctaca ggagcagaaa
      241 attgcttcat ggcaggatgt cgatcgcttt ttagtcatcc gttttctgga gaagttgcat
      301 gaagaaaaaa aagcgacagc aactgttact cggatgatct caagtctcag aagattccat
      361 cagtttttac ggcaagagcg tatcactgat catgatccga tgcaacatat cgatacgccg
      421 aagaaagcac agaaattacc aagtacattg actctgacag aagtagaaaa gctgattgaa
      481 gcacctgata cgacaaaaac gttagggata agagatcgtg caatcttgga agtcatgtat
      541 gctactggga tgcgcgtcag tgaattgatt ggattgaaat tgggagatct tcatctttct
      601 cttggactgg tacagacagt tggaaaagga gataaagagc ggatcattcc tttaggtgat
      661 tatgcgatcc aatggctgga acgctactta gacgaagcac gtccattact tgtaaaggac
      721 gcatctgaaa tgcatgtatt tgtcaataat catgggaaag gtctttctcg gcaaggaatc
      781 tggaaaaatc tgaagcagtt agtacgagag gctgggattt acaaagaagt aacaccacat
      841 acgctgcggc atagctttgc cacacatctg ctagaaaatg gagctgattt gcggacagta
      901 caggaattgt taggtcacgc ggatatctct acgactcaga tttatacaca tatcacgaag
      961 aaaagaatga cggatgttta taagcagcat tttccaaggg cttagtcata ggaaggtgag
     1021 aagatgttaa cacattaccg gaaagaaaac cgtaaaattg ctatgggact attgagtttc
     1081 cataagaaac tgacaggtaa aacttctttg ttgaaggaga ttgatacgta cgaatctgaa
     1141 gacgattatg aacttttttt ctttacgcca gaaggttcaa ccaacattca agggattatt
     1201 ggtatttact gggcaacgaa cgatcagctg atcatccatg acatttcttt gaatccgtct
     1261 tatcgcggtg agaatatggg ctttcaaatg ttagatgaat tacaggaaaa gtacccggat
     1321 gtctccatcc gtgcaactga aatcacagag ccttacattg gaaaatggct agcaaagaaa
     1381 aagaagaaag agtgaatgag ttgacggaga tcaatattaa attagatgta ttcgagggtc
     1441 ctctcgatct gctgcttcat ctgattcagc aaatggaaat cgatatatat gatataccga
     1501 tcgctacgat cacggagcaa tatatgaatt atatccatac aatgaaaaca ctcgagctag
     1561 ctgttgcagg agaatactta gtgatggctg ctacattgat ggtaatcaaa agcaagacat
     1621 tgcttcctgt ccaagaattc ggcacagacg atgaagaatt atgggaagaa gatcctcgag
     1681 aagaattggt cactcagctt ttagaatacc gtaaatataa atatgcagct gaaagactga
     1741 cagaaaaagc agaggaacga agtctttatt acacaaaaga acctatgaac atcgatgggt
     1801 tagcggatgc tgaaaagccg ttgaagcgag gacaagtcaa aaaaatcgat ttgatccgtg
     1861 ccatgcagaa aatcttagaa cgtaagaaat tttctcaaaa agtggaaaaa acgatcacgc
     1921 ccgatgacac aacgatcgaa gaacgaatgg tatttatcga agagcgtata aaaagaaatg
     1981 cgcatggctg tactttcgat tctttttttg agtcaccttc taaaagtgaa gttgtcacta
     2041 cttttatggc tttgctagaa ttgatgaaaa aagggcagat cattgcgcaa caaaaagaaa
     2101 actatgaatc aattatgctt tatccagcag taggagatac cgaacatgac gaaattaagt
     2161 gaacttgaag cccttctttt tattgttggc gaggaaggga tcagtaatga agaattaagc
     2221 ggactgttaa atctcagcga agcagaagtc acaagtttag tgaaagaatt aaaagaatct
     2281 tacactcaaa actcgcactc agctttgcat atttttgaaa cggaaagcca ttatgtatta
     2341 acgactaaaa aagagctggc tcccttactt aagcgatatg cgcaagggac atcaaacaca
     2401 ttatctcaag ccgccgtcga aaccttagca atcatcgctt ataaacagcc aatcacaaga
     2461 gctgaaatcg agcagattcg aggagttcag gtttcaggag cggtacagcg tctagctgcc
     2521 cataaactga ttgaagaaaa aggtagagta gaaggaccag gtcggccgat attatatgga
     2581 acaactggct acttcttaga ttattttggt ttgaaatcat tagaagaact gcctgataca
     2641 caagaaatgg aaatttcact tgaagaagac gtgtcaccag atctattctt tgatggaatg
     2701 aataaaggag aataattgta atggagagat tgcagaaagt aattgcccac gctggcatca
     2761 cttctcgtcg aaaagcagaa gaattcatcc ttgcaggacg tgtaaaagta aacggacaag
     2821 ttgtaaggga attagggata aaagttggga aacatgacac tgtggaagtg gatgaagtcc
     2881 caatttatca agaagaatac ggttactatt tattgtacaa acctaaagga gttatttctg
     2941 ctgttgcaga tgacaaagga agaaaagttg ttacggatct tttgccgatg gtaaaagaac
     3001 gtatctatcc tgtaggtcga ctggattacg atacttcggg gatcttgcta ctgacaaatg
     3061 atggagattt tgctcagcgc ctgacacatc caaaacatga agtggataaa gtttacgttg
     3121 caaaggtaaa agggatcgct acaaaatcga tgcttgcacc tttgacaaaa ggaataaaga
     3181 tcgaaggacg aaaaacatct ccagctaaat tccaaatttt atcagtggat cctaagacaa
     3241 accacagtat cgtggaattg acgatccacg aaggacgtaa tcatcaagtg aaaaatatgc
     3301 ttcaagcagt tggtcttcct gtgcaaaaac taaaacgtga aaaatacgga gatctgactt
     3361 tacaaggcct acgtcctggg gattaccgtg aactgaacaa aaaagaagtc agcagtttgc
     3421 tgaaccactc aaaatgaaca cttacttttt ataagtgtta gatgttgcgt tttttgaata
     3481 gatatgtata atgaatatgt aaacaaaaat tatataaggt tcgtcttcag gggcagggtg
     3541 aaattcccga ccggtggtta tagtccacga cctattttgc aaaaaaatag ctgaatcggt
     3601 gaaattccga taccgacagt atagtctgga taaagaagat agagcttatt tgacgacgct
     3661 tgatcaaata actctaactc tattttccct ggaaaataga gttttttcat ttggagagaa
     3721 acgtcaaaaa gttcgctgaa gaggagtcct aacaggagga tttcaaatga agaacacccg
     3781 tgtacagaaa atggttgcag tagcgatgtt tgctgcgatc gcattagttt tacagtattt
     3841 tgcttttccg atcatgccgg cttttagttt tttgaagatc gattttagtg atatctcggt
     3901 attgatcagc atgttcttat tcggtccagc ggccggagtt gcaacagcgt tcttgcgttc
     3961 agtattacat ctgctgacta caggtttttc tccggataat ttggtaggag acactgct
//
LOCUS       DACENJ010000198         4013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.204, whole genome shotgun sequence.
ACCESSION   DACENJ010000198 DACENJ010000000
VERSION     DACENJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.204"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            278..1246
                     /locus_tag="IW748_002311"
     CDS             278..1246
                     /locus_tag="IW748_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1105210.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1257..2072
                     /locus_tag="IW748_002312"
     CDS             1257..2072
                     /locus_tag="IW748_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ1105211.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2140..2850
                     /locus_tag="IW748_002313"
     CDS             2140..2850
                     /locus_tag="IW748_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1105212.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2886..>4013
                     /locus_tag="IW748_002314"
     CDS             2886..>4013
                     /locus_tag="IW748_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ1105213.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINYFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1342 a          592 c          870 g         1209 t
ORIGIN      
        1 aacaaatcaa gacaaactta taaaccttca tcccagcacc ataattattt aaatcatagg
       61 catttttcga aattagtata tgtactcaag tttcactgca gattatttat attttttaga
      121 gaaataaatt tttcctattg acaaacacaa agaaagcgga tacaatacaa ttatgtcaca
      181 tgacgttata tgacgttatg taatatcttt acatttaatc agttactttt attttggata
      241 aaattaacat ttttaaaatt ttaaggagga aatgttgatg gatgtagcaa aaacggttaa
      301 cgcaattgta gaaaaaaatg atggagagat ttctggaatt tatttggtgg cttgtggcgg
      361 ttcgctggta gatatgtatg tctcagatta ttttcttaaa gccgaagcat cgaagtgtat
      421 tacaggctta tatacagcga acgaatttaa ccatgtaacc ccaaagcaat tagatgataa
      481 atctgttgtc attctatgtt ctcatggagg aaatacagca gaaacggtaa aagcaggaga
      541 agtcgctcaa gctagaggag ctgtgactgt tggtctgact cataataaaa ctgcggattt
      601 attaaaggtt tctgatcata gtttccttta cgaatgggga gatgatgcaa aaatagtcaa
      661 taatcctatg gccatcattc ttgatttaac tgtatctttg gtcaatgtta ccgaaacatt
      721 tgaacattac ggtaaattca gagaaggcat gaaggttatc gataccgtaa ttgaaaatgg
      781 cattaagcag gtgcaggatc gtattaaagt gtttgctgat aagtacaaag atgaaacaat
      841 gttttatatc ttaggtagtg gaccatcata tggacatgct tatggttttt caatttgctc
      901 tttgatggaa atgcaatggc ttgattcaac ttctgttcat tctggagaat tcttccatgg
      961 accttttgaa gtaacagaca aagatacgaa ttttattctc ttgatgagca ttggacgcac
     1021 tcgtgcttta gatgaacggg caaaacgatt ccttgataaa tatgcagaaa gagtagaaat
     1081 cgttgatgca aaggaactag gattggatat gatatcagat gaagtttctg aattcttcaa
     1141 tcccatttta ttctacagta ttctttcaga atatcgttca gctttagcag atgcacgcaa
     1201 tcatgattta gatgttcgtc gctatatggg gaaagtggag tactaggagg attaaaatga
     1261 aggtattagg cttaggtgat aacgtcgtcg ataaatatga aaatctggga attatgtatc
     1321 ctggaggaaa ttcattgaat tttgcagttt ttgcaaaaaa actgggacat gaaagtagct
     1381 ttatgggagt ttttggctca gatgaggagg cagctcatgt tttatcagca ataaaaaaaa
     1441 taggactgga caacagtcac tctcgctttg aaaaaggcga gaatggctgt gccagagtga
     1501 agattgatga gggcgaccgg attttccttg gcagcaattc gggaggtgtt actcgtgaat
     1561 acccgattca gcttactgaa gaagacagag aatatttaaa ccagtttgaa ttgattcata
     1621 tgggattata tagccatgtc aatcatctat tagaagagtt acaaaatgtt tctggaaaga
     1681 tttcttatga tttttctgac gactttacgg aagaagaggt tcagcgggca attgataaag
     1741 ttgattttgg cttcttttct atagaagact tttccgatga agaagtaaag cagtttttga
     1801 tgaagcattt ttgtgagcga aatgatgtat tgattgcaac ccgaggagaa aaaacggcta
     1861 ttggttatga tggagcaaag ttttatgaag tgaaccccgt gctagaagaa ccagttgata
     1921 caatggcagc gggagattct ttcttaacag ctttcctact ttattttctc gaaggggacg
     1981 atatagtcac cgcgatgaaa aaggggaatg aatttgctgg gaagtcttgt ctggttgacg
     2041 gttcgttcgg ctttggtatt cattacgatt gagcttgttt taaattcctg catttgataa
     2101 cctaaagaaa gaaatcccgg taaagtggag gttaaaaaaa tgctggatac aaatgcacaa
     2161 ttaccattgt acttacaatt aaaagaaata ttaaaaaata aaatcaatac aggggagtat
     2221 cccgagaata cgaaaattcc cgctgaatca gagctgatag aaagttatga ggtcagccga
     2281 atcacagtga gaaaagcagt agaagaactg gtagaagaag ggtacctact taaaaaacaa
     2341 gggaaaggaa cttttgtcaa ccggcataaa gtttttcgta aaattgaata tgttatgggt
     2401 ttcagtgaga gttgtaaatc taatggtttt acaccgacta gcattgtttt ggaacgcaag
     2461 atcattccag caactgaagg gatggcaaaa aaactcgata tcgctcaagg tgatgatgtt
     2521 ttatatatcc agagaaagcg aatggcggat aacatcccca ttatgttaga aaacaattat
     2581 ttttcttatg aaagatatag tttcttaatg gaggaagagc tgactgattc tttgtatgaa
     2641 gtacttaaag tccaaggtac cttagccatc aatcctgggg aaactgcact tgaaattgtc
     2701 acagctgatg accaaattgc caaaataatg gaagtagctg ttgggacgcc ctttttcttt
     2761 atggatactt taatcaacga tcaaaacaac ataccaattc atgtaggtaa tcaatattac
     2821 ctgggagaag tatatcgttt ttacttatga tttcctgttt tgatttcaag gagtagattt
     2881 tatttatgaa gcaaatattg cggtcaaaaa tgatttttga tggagagcag ccacctttca
     2941 aaggttatat tgtaatcaat gaagggatca ttgaaaaggt ggaaaacaac tggaattaca
     3001 gtgatctatt aaatgaaaac acggaattaa ttgcttatga tgatcaattc gttatgccag
     3061 caattcatga taatcatgta tttttcagtg gttatttggc tatgaatgct ggcatagatt
     3121 taagcgatac atcttcagct aacgaggctg tagagcgtat taaagctgta atgaaccata
     3181 aaaaagcaga tgaaccaatt tatgctcatg gctgggatgc tgccctgtgg aaaactgacc
     3241 catcagaaga gctattgaat gaaataaact actttggtcc aatcacagcg atcgacaaaa
     3301 atcgcagcca ttgttggatg aatgctattg ctaagcagac ttatggtttt acagaaaaag
     3361 aaaccagtgc tgaaagtcga gtattattaa ttaaggaaat gctagcaaac aaaaagcttt
     3421 tagcagaggt ttacgctgaa tttgaagcat tactgctttc aaaaggtgtt atatcaatga
     3481 aagaaatcgt ttttgatgac gcagaatttc tttctaatat acctcagagg aaaatactta
     3541 gtaacctata tgtgcaggca gtccaagaac ctctacagca tgaacttcta tatgagtata
     3601 aggaaaaagt tttccctgaa aaggtccgct ttggtggagt gaagatcatg gtagatggtg
     3661 ttgttgcgga tagaagtgga gatatttacg gtgtttatca gtcaggagta gcttcacccg
     3721 agattgacta tgaggcaatt aaagcagaag ttgctgcatt caatgaagta gagattcctt
     3781 gctgtttgac aacggaagga gataaagcgg gttataaagc tgcgtcaatt cttgtcgaat
     3841 ttggaaagcg gttacctgaa ggcgttttta attcgattag tgatttggaa atggttactc
     3901 aaaagacagc agaaaaaatg aacgaaggac aagtagtagc agaggtatat ccacaaattc
     3961 tcggcttgaa tcaaacgtta gaagaggctt acatgtcaac tgttataaaa aat
//
LOCUS       DACENJ010000199         3969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.205, whole genome shotgun sequence.
ACCESSION   DACENJ010000199 DACENJ010000000
VERSION     DACENJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.205"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            77..691
                     /locus_tag="IW748_002315"
     CDS             77..691
                     /locus_tag="IW748_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1105214.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            684..2408
                     /locus_tag="IW748_002316"
     CDS             684..2408
                     /locus_tag="IW748_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1105215.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            2405..3856
                     /locus_tag="IW748_002317"
     CDS             2405..3856
                     /locus_tag="IW748_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1105216.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         1423 a          625 c          788 g         1133 t
ORIGIN      
        1 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
       61 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
      121 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
      181 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
      241 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
      301 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
      361 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
      421 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
      481 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
      541 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
      601 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
      661 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
      721 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
      781 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
      841 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
      901 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
      961 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
     1021 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
     1081 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
     1141 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
     1201 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
     1261 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
     1321 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
     1381 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
     1441 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
     1501 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
     1561 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
     1621 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
     1681 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
     1741 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
     1801 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
     1861 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
     1921 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
     1981 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
     2041 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
     2101 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
     2161 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
     2221 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
     2281 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
     2341 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
     2401 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
     2461 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
     2521 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
     2581 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
     2641 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
     2701 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
     2761 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
     2821 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
     2881 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
     2941 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
     3001 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
     3061 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
     3121 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
     3181 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
     3241 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
     3301 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
     3361 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
     3421 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
     3481 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
     3541 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
     3601 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
     3661 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
     3721 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
     3781 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
     3841 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
     3901 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
     3961 gataaacgg
//
LOCUS       DACENJ010000200         3937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.206, whole genome shotgun sequence.
ACCESSION   DACENJ010000200 DACENJ010000000
VERSION     DACENJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.206"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..295)
                     /locus_tag="IW748_002318"
     CDS             complement(<1..295)
                     /locus_tag="IW748_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1105217.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEK"
     gene            complement(282..644)
                     /locus_tag="IW748_002319"
     CDS             complement(282..644)
                     /locus_tag="IW748_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105218.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
     gene            842..2083
                     /gene="sstT"
                     /locus_tag="IW748_002320"
     CDS             842..2083
                     /gene="sstT"
                     /locus_tag="IW748_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ1105219.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            2230..2865
                     /locus_tag="IW748_002321"
     CDS             2230..2865
                     /locus_tag="IW748_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1105220.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            3056..3796
                     /gene="truA"
                     /locus_tag="IW748_002322"
     CDS             3056..3796
                     /gene="truA"
                     /locus_tag="IW748_002322"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1105221.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
BASE COUNT         1234 a          608 c          794 g         1301 t
ORIGIN      
        1 ccttttcttt ccagttccat tctttcaaac cataacccat taaaaataaa taaaaaatcc
       61 ataaaatact ctgtccattc tgtaatgcaa acgggtcttt attaaataga gtagagcagc
      121 caactaatcc aattgttaaa aataatagta gatatttaat tgttgttttc gataatttat
      181 ttaaaaatcc aactgtataa ggaatactac aaaaaagcaa agtacaagaa acagcataag
      241 taaagtaatt ctgactgata ggtgttagga taagccaata atcacgaata ttcaagctgc
      301 cagcttgtac gtaaaaaaaa gatattgcaa actagtgtgg ctattaggaa tataaaggca
      361 aaacgactta aataatgtaa aacttttaaa cgggcgattt tttgatattg gtaacccatg
      421 taaagtggga tcaaatttgc accaataaca acggaagttt gtaaaaatgt atagatgtag
      481 aatcgaccag ggtttgttgc atatgtagga atagtgactg ctgaaatcaa tatacgctgt
      541 aaaaccatca ttatacaagc gacaaagatt gtcaggaagc cataactctc cttctttatt
      601 tttttcaaga ataaacacct cacaaaaaaa ttggacaaaa acataagaaa acgtttcctg
      661 ataagttgcc cattaataac atcctaactt agaatatctc aaaaaacaac gtgtagacag
      721 aaaaagtaca aaattagcaa gaaatggttg tttgtattgt tagaattttt agaaaattat
      781 acctttagtt tctttgcata aaatgataca atgattgtta aaattgtgaa agggtggtca
      841 tttgttagag aagtttttgg atatgtcgtt agttcaacgt attgttgttg gtattttagt
      901 cggaatattg tttggtatct ttttccccac atggacattt atttcaatat tgggaacgtt
      961 gtttgtcagt tgcttgaagg caatcgcacc tattctggtt tttttcttga cgatggcgtc
     1021 aattgccaag cataaaattg ggaataagac attcgtgaag cctattttga tcttatattt
     1081 agtgggaaca ttgctttctg cacttgtagc agttattgta agttcaattt ttactgttcc
     1141 tattacttta caagaaacag tatctgaaaa agcaccgcaa agtttggaaa gtgttttatc
     1201 tactatgttg acgaatgtca cacaaaatcc tgttcaatct cttattgatg cgaattacct
     1261 tggcgtctta ttctgggctg tattgttggg actagctttt cgagcacgtt cagaatcgac
     1321 aaaagagttg gtggatcaga tatctatagc gttgtcacaa gtcgtacaag tgattattgc
     1381 atttgctccg atagggattt taggattggt ttatcaatca attgctacga ctggaatcgc
     1441 tggattggca gagtatctgc agcttttact tgttttgatc ggtacgatgc tgtttgtcgc
     1501 attagttgtg tacccattcc tcacgttttt gtttattaaa gaaaatccct atccattgat
     1561 tttcttttgt ttgaaagaaa gcgcacttcc cgctttcttt acccgaagtt ctgccgcaaa
     1621 tatcccgata aacatgatgt tggctgaaag attgaaattg acaaaagagt cttattccat
     1681 atcgatcccg ttaggtgcta cgatcaatat gggcggtgcc gcgattacca tttccttgat
     1741 gacgttgact gctgtacatt ctttagggat tgaagcacct tttgttttga aagtcctttt
     1801 atgtattttg tctgctttag ctgcttgtgg agcttcaggt gttgcgggcg gttcactgtt
     1861 attgattcca ttagcatgta gtttgtttgg tatctctaat gatgtcgcga tgcaaatcgt
     1921 cggtattgga tttattatcg gtgttatcca ggattcaatg gaaactgcct taaattcgtc
     1981 aagtgatctg ttgtttactg caataggaga gctaggcgcg cgtaaaagaa gtggggaaaa
     2041 aatcaaattg aaagattgtt tagatggatt aagtgaacgt tgaacgttag atatttagta
     2101 tttttaaaat gattcgagtt tttaatgagt tatatagatg ttcgtgtaaa tagaggtttc
     2161 tgttaaatca agataaagtc gtttataata acaattaaaa gcataggtaa ttgttattgg
     2221 aggcgtatca tggatttttt acgtaaattt ggttttttct ttttattatt aggcgtgctg
     2281 agtgattttt tgactccgta cgtattagga ttgtattatc ctgaattaga tcaaatgaag
     2341 agcgtgatca gtgtatttgg agaggtttcc agtcctgtaa gaaaagcgtt tcttatttgg
     2401 tctgtggttt cagggatctt tttcatcctg tcattgcctg cattgtatgc tgcatttgta
     2461 agtacatctc gtgtgttagc taccctagtt acagcagcca tcgcgttcta tggtataggt
     2521 gattgtatct ttacaggttt gttcagtatc aatacccatg aatcttcgtg gaatgtctcg
     2581 acatgggtcc ataatatcgg atcaggttta ggttacagtg gttttttatt gtttccagtt
     2641 cttcttttct tgatctatcg aaaaaatggg caaatggacg taagccactt ataccttgga
     2701 ctgtctgtga tcagtttgct ttttgcggcg atttatggac tggcaagaat tccttctata
     2761 aattcttggc cattactgaa taaaataggt ttttgccaaa gaatcagttt tttctttaat
     2821 tatgtgccca ttgcctggtt gagtatcttg catctcaaac agtaaaactt gagccgtcaa
     2881 attgaacagt cagcggatac ttcatccggt ggctgttttt tgtttttctg aaatatatat
     2941 ttccacagtt ttcttgtata gcttctgttt tagagaagaa aggagctata ttttgtgcta
     3001 caatcttaaa aaggtagaga gaagaccaaa tggaagacag aggagccact aaaatatgag
     3061 aaacatcaag ttgacaatcg aatatgacgg aaaaagatat tcaggatggc aaagacttgg
     3121 agatgacgat aaaaccattc aaggaaaaat cgaaaaagta ttgcatcaga tgacaaatga
     3181 agagatagaa attattggat caggaagaac agatgctggc acccatgcgc gaggacaagt
     3241 agcaaatttt aaaacaaaca cagaaattgg actggcagaa atgatcgact ttatgaatcg
     3301 ttaccttccg cgggatattg tcatcaaaag aatcgaagaa atgcctgagc gcttccattc
     3361 aagatataat gcggtgggca aaaaatacag ctattatgtt tggaacaacg tgattccttc
     3421 agcatttgag cgaaaccaca gcttttattt ccctcaagag ttggatatgg acaagctgaa
     3481 tgcagcttgc gaaaaattag tgggtacaca tgatttcatt ggtttttctg cattgaaaaa
     3541 atcaaaaaaa tccacagtgc gtacgattga tgaattatct atcgaacgaa agggagaaat
     3601 gcttcatttt acttttgtcg gagacggatt cttatataaa atggtgcgga ttatcatggg
     3661 gacattacta gaaatcggca caggtgccat gccgatagaa acaatcgatg aaatatttga
     3721 aagcaaagtg cggcaacatg caggagaaac cgtccctgca caaggtctgt ttttggatga
     3781 agtttattat cgataaaaat actttgataa tggatgaaac gcatgtttca cagtataaat
     3841 atcgatgtta aataatgtat gaagcaaatt aaagaaatat aaaaaaagac tttttagcct
     3901 gtttatgtgt acagctaaaa agtctttctt aaataaa
//
LOCUS       DACENJ010000201         3933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.207, whole genome shotgun sequence.
ACCESSION   DACENJ010000201 DACENJ010000000
VERSION     DACENJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.207"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..1188
                     /locus_tag="IW748_002323"
     CDS             4..1188
                     /locus_tag="IW748_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1105222.1"
                     /translation="MLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKV
                     ILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALS
                     PVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLI
                     AMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREI
                     FDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSA
                     FGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAG
                     VLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKN
                     HR"
     gene            1154..1504
                     /locus_tag="IW748_002324"
     CDS             1154..1504
                     /locus_tag="IW748_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105223.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1596..2015)
                     /locus_tag="IW748_002325"
     CDS             complement(1596..2015)
                     /locus_tag="IW748_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1105224.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2016..2360)
                     /locus_tag="IW748_002326"
     CDS             complement(2016..2360)
                     /locus_tag="IW748_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1105225.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2626..3585
                     /locus_tag="IW748_002327"
     CDS             2626..3585
                     /locus_tag="IW748_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1105226.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            3718..3924
                     /locus_tag="IW748_002328"
     CDS             3718..3924
                     /locus_tag="IW748_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1105227.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT         1125 a          725 c          738 g         1345 t
ORIGIN      
        1 ggattgctaa ctattcttac ctcgccaagc aatcttttga cagattattt tgctttaggc
       61 ggtttcggaa gcgcattcat aaatgtaggg attttgacct tactttctgt gcttttagct
      121 tatcggcaca aggtgatttt aaacggtccc ttatttgctt cgattctaac agttaccggt
      181 ttctcttttt ttggcaaaaa cctttataat tctatctcaa tcattttagg tgtgtatcta
      241 tacgctgtat ttgtcaataa acctttttct cagtacatca tgatcggact gtttggtagc
      301 gcattatcac cggtcgttag ttatatcact tttggtatgc gttttccttt actgataggt
      361 attttattgg ggaatttagc tggcatagca attggtctac ttcttccgcc cttagctgct
      421 caaactttag ttttccatcg tggattcaca ctttataata ttggattcac ctctgggttg
      481 attgcgatga cttttactgc tgttttgagg ttattcagct attcaatcgt cgaaaataca
      541 ttggttttca atgaatatca ctttcctctc atttggatta tttttggctt tttttcgctg
      601 acagtcggaa ttggtttcta ttataattcc tttcggcttt tggggattcg agaaattttc
      661 gattcatctg gaaaattgac aactgatttc attgctaatt ctggtattgg tgctactttg
      721 atcaacatag gcttagttgg tctgatgctg tcttcttatg ttttgctggt tggcggtcag
      781 ttaaatggac ctgtcatcgg tgctatttta tctgcggtgg gtttcagtgc ttttggttgt
      841 catttaaaaa acagttttcc gatactagtc ggaatattta ttgcttcttt gtttggaact
      901 tttcatgaga tcaccagtac tggcatgctt gttgcagctg tgtttgggac aggtctcgcg
      961 cccataagcg gtttttacgg cagtttttac ggtgtaatag ctggtgtact acatattgct
     1021 cttgtccata acgtcagcac tttgcacgga ggactaaatc tatataacag tggtttttca
     1081 actggtttcg ttgcaggtat ccttgtaccg atactagata actttaccgc tgtcagaaag
     1141 gaaaaaaaga cacttggaaa aagaattatc aaaaagaatc atcgatgagc ttcttactta
     1201 tttatattcc catgaaataa ctgaaatacg tattggagta gaatttgctg aggaagggct
     1261 tttcatagag atacagggta cggcagaaac tcagccagaa gatttagatg tacttgctga
     1321 tttgctgaac aatcctcggg accctagtat ggaaatctat tatcaagaac tattaggtaa
     1381 tccccgacat gatcgggaag attatcatct gttaggtagt ttgattgatg acgtggaaat
     1441 catgtatgcc cagccgatta tcaatattaa gatctttcga cgtgcttctc attcctatta
     1501 ttaaccctat tttctattta taaaagactg aatcaaaaaa gcagctcctg aaagttcgat
     1561 taaaatctac tttcaggagc tgttcaagct tgacattatt gattttccaa tccttcatac
     1621 acttcatctg ttacaggttc taaaaactct gaattaccag cagtgatcgc caaatgttca
     1681 aaccaactgt cttttgtcgc accatgccaa tgtttaatcc ctttgtgcgt tacgacgaca
     1741 tctccagcca ttaatcgtct tgctggcttt ccttcttcct gataccagcc ttctcctccg
     1801 gtcacaagta agatttgata gccgtcttta tggacatgcc agtgattgcg acaacctggt
     1861 tcaaaagtta cattacctac accaacagaa acttcgggat cattcactaa catattcaga
     1921 tagctttgcc cggtaaatac ttgagcgtaa gcttcgtttt tttcacctgc tggaaaaatc
     1981 acgccattgt gtatttcttc gtgttttgtc gtcatttacg cctctttctt atcttcgcta
     2041 ttttcttcta aatagatttc ttttgctaaa ctaaaagctg accaagcttt tggccatcct
     2101 acataaaaag ctaaatgggt aatgatttct gctatttctt cactagtaat gccattttgc
     2161 ttgccaatct tcagatgagc tggcaattgt tcaaaatttc ctcctgaaat taatgcagat
     2221 atggtgatga tactacgctg atgagcagat aattcttctc tggaccatac ttgtccaaat
     2281 aaaacatcat cattcaattc tgcaaatttt ggtgcaaaat ctcctaattg atctcgtcct
     2341 gcggtttgtt tttctgccat ttctcttatt ctcccttcac gactgttagg tgattcaact
     2401 atggttctaa ggcaacttta ttttctattg tttcttcgca tttcatttta aatgtagtct
     2461 ttcagcctgt ccaatgctta gtttattcag tcgatatgct ttttaagcat agttgctaac
     2521 gcttttttta ttcgccgatt gtaaaattaa gctagacaac taaaaaagtc atttgtgcta
     2581 cactcaaaat agttccgacc aaagaattat aaggagtgag tacaaatgac ctatacccat
     2641 cttacaacgg atgaacttgt aataatagag tcttatttca aaatgaatca atctgttgct
     2701 aaaactgccc attgcttgaa tcgttcaaga caaacgatcc ataaagtata tctgttcttc
     2761 aagcaaggaa aatcagcctt agaatattat caacagtata agaaaaacaa atcaaactgt
     2821 ggtagacgtc cgcttgtttt acccgaggaa caatcagaat atattcaaag aaaggttgtt
     2881 caaggatgga cacccgatgt gattgttggt cgtgcagcgt ttcctattag ttgttctgct
     2941 cgtaccattt atcgtatgtt caaaaagggg ctatttgatt cttctgactt accgatgaaa
     3001 ggcaagcgta aaccgaatgg acatcaagaa agacgtggaa aacaaacttt ccgccgctct
     3061 attcatgaac gtgaaaagga ttatagccaa ttctcaaatg agtttggtca ccttgaaggt
     3121 gacactatcg taggtctgaa acataaaagt gctgtaatta ccttagttga acgattatca
     3181 aaagttatca tcacattgaa accgtgtggt agacaagcga ttgatattga aaaaaaatta
     3241 aatcaatggt ttgaatctgt accgaaaaac ctattcaaat ccatcacttt tgattgtgga
     3301 aaggaatttt caaattggaa acagatcagt aatgtcaatg atattgccat ttatttcgct
     3361 gatccaggaa cgccgtctca aagaggccta aacgagaatt ctaacggatt gttacgtaga
     3421 gatggtttat tgaaatctat ggatttcaat tcagtagatg aattttttat tcaatctgtc
     3481 gcatctaaac gaaataatat tcctagaaaa tcactggatt atcgaacacc tttggaagta
     3541 tttttgagtt acgtaagtat tgatgatctg tctaacttaa tttgacaatt aagaaagata
     3601 aaattgaaca actggacggg caaattgatg ccttgctttc agaaagagaa atattgatag
     3661 aagaaaacaa gcgattgaaa aagaaagtag cccaaccaat gaagggaggt ggatagtatg
     3721 aacaatagaa cctttgatat tttgaaatgg atcgctatag tagttatccc cgctttagcg
     3781 acttttgtcg gtcttgtcgg aaaaggtgtt aactggcggt acacagatat aacggtgatg
     3841 attattacag gttttggagc atttctaggg agtattttag gtgtatcaaa tcgaacgtat
     3901 aagaaaattt catcagataa ataggaggat aga
//
LOCUS       DACENJ010000202         3850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.208, whole genome shotgun sequence.
ACCESSION   DACENJ010000202 DACENJ010000000
VERSION     DACENJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.208"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            29..3721
                     /gene="nifJ"
                     /locus_tag="IW748_002329"
     CDS             29..3721
                     /gene="nifJ"
                     /locus_tag="IW748_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1105228.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
BASE COUNT         1264 a          717 c          876 g          993 t
ORIGIN      
        1 gagagaatat gaacaaggat gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac
       61 ggcagccgca tatatttctt atgcatttac cgagattgct gcaatatatc caataacacc
      121 cagttcgacc atggctgaat tagtagatca atggtcagca gcaggaaaag aaaacgtatt
      181 cggtcaacca gtaaaagtag tagaaatgca atccgaagca ggagcagctg gtgtggtcca
      241 tggatcattg aaaactggtg cactgacaac tacatatacc gcttcacaag ggctgctttt
      301 gatgataccg aatatgtaca agattgctgg ggaactactg ccaagtgttt tccacgttgc
      361 tagtcgggca gtgacgacaa atgcgttgaa tattttcgga gatcacggag acgtgatggc
      421 tgcaagacaa acaggatttg ctatgttagc tgaaagcagt gtacaagaag tgatggattt
      481 agcacctgtt gcccatcttg cttctattga aggagaagtc ccttttttga atttctttga
      541 tggattccgt acgagtcacg agattcaaaa aatcgaagtt ttggaatatg aagaattagc
      601 tcctctgatc aatcaagaga aattagcttc atttcgtaaa cgagggatga atcccaatca
      661 tccgactgtt agtggtacga accagaatcc agacatccat tttcaacaac gggaaacgat
      721 caacccctat tatgataaat tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg
      781 attacgagga acttcttatg acttggtgac ttattacgga gcagacgatg cggaagaagt
      841 cattgtttct atgggatcag ttgcacagac gattgaacaa acgattgatt acttgaatgc
      901 aaacggacgg aaaacaggtt ttttgaatat ccatctttat cgtccttttc cagtggaaac
      961 attcgtgcaa aagttgcctc atacagtaaa aacaattgct gttcttgatc gaagtaaaga
     1021 accaggggca ggtggagaac ctctcttatt agacgttcaa agtgcgttat atgattctga
     1081 acttcgcccg gctgttattg gtggtcgcta cggattgggg tctaaagatg tgactcccga
     1141 ccaaatctca gcagtttttg atgaattgaa aaaagaccca agcatacgca aaaaacggtt
     1201 taccataggg atcgtggatg atgtgactta tcaatctttg gaaccaaagg aaagtctgga
     1261 tttgacagaa ccgcaaacat tccaggccaa gttttgggga tttggatctg acggaacagt
     1321 tggagcaaat aaatcagcta tcaagattat tggagatcac accgataaat atgcgcaagg
     1381 ctatttttat tatgattcga aaaaatctgg cggtctgact gtctctcatc ttcgttttgg
     1441 agatacgccg atacgttcag cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac
     1501 accagcttac ctgcatagct atgacttggt caaaggattg aagccaggtg gcattttttt
     1561 attgaatact ctttggagcg acgaacaatt agaaacgcat ctgcccttaa aattaaaacg
     1621 ttacttggct gaaaataaca ttagattcta tacgatcaat gccatgagat tggcccaaga
     1681 agtcggtttg ggacgccgga tcaatacggc aatggaaacg gcttttttta aattagctga
     1741 catcattcct tttgatgagg tgcttccgct gctaaaagaa gaagccctga aaagctatgg
     1801 acataaatca atggctatcg tagaaaaaaa catacaagca atcgatcgga cgaccgaact
     1861 gcttcatcaa gtcgcgatcc cagaccattg gagaacgttg gagatgcctg aaaaaatcaa
     1921 taaaaggaac actggttata tacaagaaat acttgaacca gtgaatgcac aggaaggaaa
     1981 tcaactctct gttggtacac ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg
     2041 aacggcttct atggaaaagc ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg
     2101 ctgtacgatg tgtaatgaat gtgcctttgt ttgtcctcat gctgccatcc gtccattttt
     2161 agcagatgaa gaggaaatgg aagaagcgcc agaaggattt atcgtgagag aaatgcgagg
     2221 cgcagacggc gtaaaatacc gtatccaagt ttcagtggaa gactgtacag gttgtggtct
     2281 gtgtgtagag gcttgtcccg caaaagaaaa agctttagtg atgaagcctt atgaagaaca
     2341 gaaagaacaa gctgtgaatt gggcttttgc aatgacatta agacaaaaag caaatccagt
     2401 caaaggaaag aatactgtct tgtctacaca attcaatcaa ccgcttcttg aattctcggg
     2461 cgcttgctca ggctgtggcg aaacacctta tgtaaaatta ttgacacaaa tgttcggtga
     2521 tcgaatgatg atcgcgaatg cgacaggctg ttcttctatt tggggcggtg cttctcctgc
     2581 ttctccttat acgaccaatg aatgtggtca agggcctgca tggtcaaatt ctttattgga
     2641 agataatgct gaatttggtt atggtatgct tctagcggca cagactcgtc gcgaggcttt
     2701 ggcaagttca atcaaagaag tattgccaaa agtatcagaa gaactacgag aattgatgga
     2761 agactggtta gtacacatgt ttgagagtga aggaacgcag caaagagcag caaaactgca
     2821 agctgcgatc gaaagcgaac gatcaatttt ccctgaactg gaggatatat acaagagaaa
     2881 ggatctattc gtaaaaaaca gccaatggat gattggtgga gacggatggg cttacgacat
     2941 tggttttggc gggatcgatc atgtacttgc cagcggagca gacgtgaata tccttgtttt
     3001 ggataacgaa gtctattcca atactggcgg tcaaacctca aaagcaacac cagcttcggc
     3061 aatcgctaaa ttttcggcta gtggaaagta tgcttcaaag aaagatctag gaatgatggc
     3121 gatgacatat ggaaatgttt atgtggcgca aattgcttca ggtgccaatc aaatgcaaac
     3181 gatcaaggca tttgaagaag cagaaaagta tcctggtcct tctctgatta ttgcctatac
     3241 gccttgtatc acacatggct tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga
     3301 agctgtttct tctggttatt ggtcattata tcgatataac cctacaatgg tcgaaaaaaa
     3361 caagaaccca atgactttgg actacaaaaa acctgatttc tctgctatgc ctgatttcat
     3421 gaggaaacaa gttcgctttt cctcgctaga taacgcaaat ccaaatcttg caaaagcttt
     3481 atttgaaaag actgtccacg acgctaaaac acgtttttac aactatgctg cactgacggg
     3541 agaattagaa aaaatcaaga aacgacttga accagaggaa tctatcgatg tacccgttaa
     3601 gactagaaaa gaaaaaacga cggaccttga agcagaagaa agaagagctg ctagaagagc
     3661 tgccagagca gaacgacgta aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata
     3721 acagaaaaaa tataatgaat aattctctga agtttaaaaa tatttattgc agagaaaaat
     3781 aaaacgcata cttgcaattt tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca
     3841 tatcagcctc
//
LOCUS       DACENJ010000203         3817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.209, whole genome shotgun sequence.
ACCESSION   DACENJ010000203 DACENJ010000000
VERSION     DACENJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.209"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            352..1041
                     /locus_tag="IW748_002330"
     CDS             352..1041
                     /locus_tag="IW748_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1105229.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IW748_002331"
     CDS             1028..2236
                     /locus_tag="IW748_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1105230.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IW748_002332"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IW748_002332"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1105231.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1258 a          658 c          771 g         1130 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaag
//
LOCUS       DACENJ010000204         3803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.210, whole genome shotgun sequence.
ACCESSION   DACENJ010000204 DACENJ010000000
VERSION     DACENJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.210"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..654
                     /locus_tag="IW748_002333"
     CDS             <1..654
                     /locus_tag="IW748_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1105232.1"
                     /translation="IVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLELLGTSFVN
                     NRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIFAAFNVS
                     FGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQVLFIGGA
                     GALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYYPGKGQ"
     gene            656..1600
                     /locus_tag="IW748_002334"
     CDS             656..1600
                     /locus_tag="IW748_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1105233.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1799..2443
                     /gene="pcp"
                     /locus_tag="IW748_002335"
     CDS             1799..2443
                     /gene="pcp"
                     /locus_tag="IW748_002335"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1105234.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2522..3799
                     /locus_tag="IW748_002336"
     CDS             2522..3799
                     /locus_tag="IW748_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105235.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1215 a          668 c          845 g         1075 t
ORIGIN      
        1 attgttattc tcgggtttgc atttaaatta gactcgatat tgatcgtaat ggttgcttta
       61 gttgttacag ctttgactgg cggtcttggc attcaaggaa tgttagaact tttaggtaca
      121 agttttgtaa acaatcgcgg gatggcgatt tttatcatca ttatgttggc aacaggaacg
      181 cttgaaagaa atggtctgaa agaatcagcg gcttccttga tcaagcgatt caagaaagta
      241 tctgctggta tgattgtaga tatttatggg atctttcgaa tgatctttgc ggcttttaac
      301 gtgagttttg gtggagttgc tggatttgtt cgtccaatca ttttaccgat ggcattgggg
      361 accatcgaat caaaagggtt agaagtccat cctaagcatg aagaagaact aaaaggtatg
      421 gcttcagcta tggaaaatgt ctgctggttc tttggccaag tactattcat cggaggagca
      481 ggggcgttgc tagtccaatc aacactgaaa gatctaggat atgacgtaac attgggacag
      541 cttgctttag tggaagttcc agtggctatc acggctttag ttgtggcaag tgtctacttc
      601 tatcttaaag aaaagaaatt agcgaagaaa tattatccag gaaaggggca ataagatgga
      661 cttttttaca aatcctgata ttttattagg cgataaatta ctggagatcc tttatatcgt
      721 gatgggactc gtattgattt acactggagt aagaaattta acagataaaa ccaatcctca
      781 tcgttatggg tcagcctttt tctggacggt cttaggggta gttattgcag gaggaagatg
      841 gcttccggca atcgtgaacg gtggcttgat ctttgcgatg accataccag caattgctaa
      901 gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga
      961 caaactagga atgaaaattt ttattccagc tttgagtatc ggtgtctttg cgatcttgtt
     1021 tgctctattt accaatttag gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat
     1081 gctaatcttg atgttctttt ctcgagataa tcgaccaagt acatttttag atgatgcagc
     1141 agatatgctg ggcacagtag ggccgttaag catgttgcca atgcttttag cttctctagg
     1201 tgcggtattt acaagcgctg gagttgggac agtgatttcc aatgcagtag gtacgatcgt
     1261 tccagaagga aatgtgaaca tcggcatcat catttacgct cttggcatgg tgatatttac
     1321 ggtaatcatg gggaatgcat ttgcggccat caccgtgatg acggttggga tcggtgcacc
     1381 attcgtattt agtcatggag cgaatccagc gttaatagga atggttgcat tgacttgcgg
     1441 tttttgtggg accctgctga caccgatggc tgctaacttc aatatcgtgc ctgtagcgat
     1501 gctggagatg aaagacaaat acggagtgat caaaaaccag ttgtttattg cgttatttat
     1561 gctagttttc caaattgctt atatgattct atttaaataa tagaagagag gggttgtgac
     1621 agaataattg tcgcaacctc ttctaacatt gtagtggaac actatttttt tataagaata
     1681 tattaagttt gatctgttat gttatttaaa ctattgtttc ctttatttac gctgttcaag
     1741 gaaaatggta tgataaaaga taaccatatg ttaaatcaat cggacggagg aacaacagat
     1801 gaaagtacta gtaacaggtt ttgatccatt tggtggggac aaagtgaatc cagcctatga
     1861 agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa atcatcaaag tggaagtacc
     1921 gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc atccaacagc accaaccaga
     1981 tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc
     2041 aatcaatcta gcagaagcaa gaatccctga taatgaagga aatcagccat tcgatacggc
     2101 tttagaagaa aacggtccag ccgcttactt tacctcattg ccaatcaaag caatgacaaa
     2161 aaatgtacaa gatcatggtc taccagcgta catctcttat acagcaggaa catttgtctg
     2221 caatgatatc atgtatcgtt tgcttcattt gatcgaaaca caattcccgg caattcgagg
     2281 tgggttcatc cacgtgccat tctcacccga tcaagtgatc gatcgtccag tgggtacgcc
     2341 ttctatgtct ttagaagaca ttgcttcttc tttgacttat gcagttgaag cagcagtaga
     2401 aaataaagaa gatatttccg gaaatgccgg aacaactcac taatttagca agaaacactg
     2461 gctggttgct aacgttatgt tagtagccag ctttttgtaa ttagaaagga aagaaacagc
     2521 cgtggaagaa tggcaatccg tctttgaaga atggtttcca aaagaaatca gcaaaagcta
     2581 tcccatcaaa atttctaaac aatacacaag cagtcaacgt tgggagatct atgcaaaatt
     2641 aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga tatctaatca gctctcgttt
     2701 tatggaagaa cattatttag ctgcaacaga ttgggtgttt tctgacttca agatcaatcc
     2761 gttttttcga acaaaacgaa gccagcaaaa attgtattgt gagtgtggta gagaattaaa
     2821 agtgcaatac atcgttaaat cgccaaaaac cggaaagatc ctaaaactag gcatcaacca
     2881 ctttgccgat cacctgcatg tctctccgac agtggcagct tcgatccatc agggaatgac
     2941 aaaagttgat ctggccttag acgaattatt atggcttaag cagaagaata tcgattttcc
     3001 tgaaggacta tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc
     3061 atatttaccg gatataaaat tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc
     3121 gatttacata gcggattatc aagcattgga aaatgaaatc aagaaaataa gtgaacacat
     3181 caatgggcag tcaaaaaagc gacagataaa aaaagaacta tttgacgact ttgcagaaga
     3241 attagtaaag gatgtagaag aattcttgat caactatcgc gcatttttac gtaaagattg
     3301 gcaatccatc gtatatgaag aagtgccagt acatcctaat gcttattttg aaacatttat
     3361 ttccgtgttg agaaagacaa aacgtcaacg aacaccagaa gtcactgctc agatggaata
     3421 ttttgcgaaa aaccaacgat tcatccaacc aaaaatctat ctatttattt ggaagcaata
     3481 ttgtcactat ggttttacag aagggttctt cgacagcatt cctcggatag tcagaaatgg
     3541 ttttttaaaa gtattgcgta aagaacgaga agctatacaa tctgctgata agaaagaccg
     3601 tacagtctca aaagaaaaat ggcaactagt agtgaaagat atccaatctg gaaatgtgca
     3661 agagacaatc gacaaatgga aaggaaaaca ttatcgtttt actgaagctc agaaacaagc
     3721 gttggaatat tatcagaagt tagaagaatc actgcgtttc aatgatgagg caagaaagta
     3781 tctgaaggaa ttactataaa aaa
//
LOCUS       DACENJ010000205         3803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.211, whole genome shotgun sequence.
ACCESSION   DACENJ010000205 DACENJ010000000
VERSION     DACENJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.211"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..775
                     /locus_tag="IW748_002337"
     CDS             179..775
                     /locus_tag="IW748_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1105236.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IW748_002338"
     CDS             899..2698
                     /locus_tag="IW748_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1105237.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IW748_002339"
     CDS             complement(2976..3188)
                     /locus_tag="IW748_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105238.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1110 a          647 c          680 g         1366 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gag
//
LOCUS       DACENJ010000206         3789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.212, whole genome shotgun sequence.
ACCESSION   DACENJ010000206 DACENJ010000000
VERSION     DACENJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.212"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            203..982
                     /locus_tag="IW748_002340"
     CDS             203..982
                     /locus_tag="IW748_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105239.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            1028..1972
                     /locus_tag="IW748_002341"
     CDS             1028..1972
                     /locus_tag="IW748_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1105240.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1988..2767
                     /locus_tag="IW748_002342"
     CDS             1988..2767
                     /locus_tag="IW748_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1105241.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            2779..3477
                     /locus_tag="IW748_002343"
     CDS             2779..3477
                     /locus_tag="IW748_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1105242.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3505..>3789
                     /locus_tag="IW748_002344"
     CDS             3505..>3789
                     /locus_tag="IW748_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1105243.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDE"
BASE COUNT         1360 a          701 c          784 g          944 t
ORIGIN      
        1 attaatgttt tatttacttt taaatcataa attgccctag tcaagaaaac agtttgtggt
       61 atgattatat agggaaaact tgtttacaaa tatttctgtg aatatattag tttaattatg
      121 ttttttaaaa taaaaagctg tcatttttat gtaagtgaat tatatcttaa tttatctatc
      181 atcaatactt aggagtgaat ctatggaaaa gccattcgtc aagtcattga ctgctgtggc
      241 ggtaggtgca ggtgctattg cgatctgttt gtttggttac catatcaata atcagcggca
      301 acatcagcaa cggatcaatt atgcagaatc tgccatcacc aatcaaaaag acaccttgac
      361 ttctttatca aaagaagtag acaagctgta tagtacaaaa gaaaaaattt ttctaaaccc
      421 tgaaataaca gaagaaacaa tcacaagttt gtcaaataag ctatcctatg tcaaactttc
      481 agcagatgat tacgacatca aagaatcaga attgcctaaa gaagctgttg caatccaaga
      541 tgagaagaaa gcaatcgtga cacagttaga tagtgcagaa tcaaaactga agatccagac
      601 agctgtcaac gatttgttta ctaaaaatgt atcaaactgg cagcaagctg tagacgatgt
      661 catcatcaaa gaaaaggtga caagtgcaga tgtcgctcgt gtacgtgaaa atatgagctt
      721 ttttaaagat agtccttgga aaacggttgt tatgcaatat ttaggattcg ctgatactca
      781 gatcgcgcaa gtcacgcaac tagatcagtt attcgatacg atgttgaagg atggtcaagt
      841 gactgccacc gctacttacg atcaatattt gacggcgctc agtcaaatag agcagatccg
      901 gaatgaaaaa ataagcgcag catacgcaac aaaagcacaa actgttgctc agcaaatggg
      961 ctatagcaat ataagttatt gacagttaaa acgttgattc aagcaaggtt agcaaacgga
     1021 ggaaactatg gcaagacgaa gaagaaaatc aagagggaaa aaaatatttc tgattatttt
     1081 agcagttctt atcgtattag tattagcaat tgtaggagta ggagcaaaat tatatctgga
     1141 tctctcacat tctattgaga aaacttacga atcagtggaa cgaacccaag gagaacgaac
     1201 gaaaccagtt gatctggaca aacaagaatc tttctctgtt ctattgatgg ggattgatac
     1261 tggagattta ggtcgggtcg atcaaggaag atcagatacg atgatggttg ccatagtcag
     1321 tcctgaagac aaacagacaa cgatcgtcag cattcctcgt gatacgtatg tcgacatcgt
     1381 aggtcgtgac acaaaagaca agatcaacca cgcttacgca ttcggcggag ctgccatggc
     1441 gatggatacc gtacaaaaat atgtagacat cccaatcgat cactatgtct ctatcaatat
     1501 gaaagggcta aaggaacttg ttgatgcagt tggtggtatc gaagtcaata atgacctcac
     1561 tttctcacaa gatggctatg actttacaat cggtaaaatc acattgaacg gggatcaggc
     1621 attatcttat tctcgtatgc gttatgaaga tccaaatggt gactatggta gacaagagcg
     1681 tcaaagaaag atcgtagaag gtgtagcaaa aaaagtattg agtttaaacg gtgtcagcaa
     1741 ctatcaatcc gttttgaatg ctctagagtc gaatatgaaa acagacatga gctttgatga
     1801 aatgaaaaaa atcgctttag attatcggga ggcatttggt acgatcaagc aagatcagct
     1861 gcaaggagaa ggctttatgc aagataatat ctcctatcaa aaagtcagtg atcaagaact
     1921 tgatcgtgta aaaaaagaat taaaagatca aatgaatcta gagaataaat aggaaagtgg
     1981 agaaaaaatg gaagagacaa tcaacttgga agaaattttt gaaacattac gcaaacactt
     2041 ggtaacaatc atcatctcaa tgtttgctgg cttggccatc tcaggaatcg taacattttt
     2101 catcttgaca ccaaaataca gttcacaagc acaattgatc gtgacactgc cacaatcaga
     2161 cacagcaaat gtcaatgatg tcaatacgaa cctgcaaatg atcaatacgt ataaagatat
     2221 gatcgtcagt gatttagtac taaatgaagt caaagatcgt ttagaaacag aagacaatgt
     2281 gaaaatgact gctggacaaa tcaaagatgc gatctccgtc aatcagtcac aaaattcaca
     2341 aatgttctcg atccaggcaa taagcacgaa tgcagtgact gctcaacaga tcgctaacac
     2401 aacttcacaa gttttccaag agaacgcaaa agacgtgatg aatgtcgata aaatctcgat
     2461 tatttcaggt gcagttgcca atacgacacc tgtttcgcca aataataaat tgaatctagt
     2521 gattggctta gtattaggaa tgatggtagg cgtaggtctg gcattcttat tcgaattact
     2581 tgatcggaca gtgaaagatg aaaaatatgt gacggatgca ttaggattcc caatcttagg
     2641 aacagttcca gaaatgtcag cgaaagaatt gaacgctggt atccgtaaaa aaggaacagc
     2701 tcctgcacga ccaaaagcta caaacgataa aacagcccca tcacgcagaa gccgctcaag
     2761 agtctaggag gaagaaaaat ggcacgaaca cagaaacaaa aagcagcaca aactcatgca
     2821 gtcagtttga tcacattaac aaacccatct tcaccaatcg ccgaacaata tcgtacgatc
     2881 cggacgaata tccagtttgc ctccgcagct ggtcagcaaa tcaaaacgat tgtcgtgact
     2941 tcatcaggtc ctggagaagg aaaatccacc actgcagcaa acattgcagt cgtttttgct
     3001 aaatccggtc aacgagttct actagtcgat gcagatttac gtaaacccgt cgtctataag
     3061 acattccagc tgaacaatgc caggggatta agtacagcac ttagttcatc aggaagtgta
     3121 gcagatgtaa tccaacgaac gccagtcgag aacttgtcga ttttgcctag tggacctaag
     3181 ccacccaacc catcagaatt gttaagttcg ccaagaatgg atcagatcct agacgaagca
     3241 agacagttat ttgacgtggt gatattcgat atgccaccag tagtagccgt aacagatgca
     3301 cagatcatgt cttcaaaaac ggatggcact ttattggtcg tacgtgaaaa tacatcacgt
     3361 aaagaatcat tgaataaagc aaaagaactg ctagagatgg tacaagcacg agttttaggt
     3421 gttgtataca acggagcaga acatagcaaa gatgctggtt attactacgg aaattaagga
     3481 ataagaagag aggagacaag agaaatgatc gatctacatt gccatatcct accaggaatc
     3541 gatgacgggg cagaaaactt agaagcaagt attgcaatgg ctgaaaaagc gattcagcaa
     3601 ggcatcacgc atattttatg cacaccacac cacaataatg gaaaatacag caatgaaaaa
     3661 tcacaagtca tttctctagt cgcttcgtta caagccgaat tagagaaaag acagttgcct
     3721 ttgacgttat tggaaggaca agaagtacgt attaccggaa cgctcatcga agacatccac
     3781 cgagatgaa
//
LOCUS       DACENJ010000207         3761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.213, whole genome shotgun sequence.
ACCESSION   DACENJ010000207 DACENJ010000000
VERSION     DACENJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.213"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..446
                     /locus_tag="IW748_002345"
                     /pseudo
     CDS             1..446
                     /locus_tag="IW748_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(974..1315)
                     /locus_tag="IW748_002346"
     CDS             complement(974..1315)
                     /locus_tag="IW748_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1105244.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1401..2108
                     /locus_tag="IW748_002347"
     CDS             1401..2108
                     /locus_tag="IW748_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1105245.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2095..2418
                     /locus_tag="IW748_002348"
     CDS             2095..2418
                     /locus_tag="IW748_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1105246.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2507..3541)
                     /locus_tag="IW748_002349"
     CDS             complement(2507..3541)
                     /locus_tag="IW748_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1105247.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTRAGS
                     NQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKILV
                     INGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELGI
                     DQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMFA
                     KTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHMLG
                     KVVVSGPFNG"
BASE COUNT         1087 a          791 c          672 g         1211 t
ORIGIN      
        1 gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc aagtacttgt
       61 ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat gaaaagacca
      121 acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac atggctgctg
      181 tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt tccgaactct
      241 cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca gagattctta
      301 tagaacatcc gcactttatc gaacgcttca aagttaagat gccatttcat gcaataaatt
      361 attcagataa attaacaact atcatccata aatacgatca tattatcaag aaaaacaaaa
      421 acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa gcagttgaat
      481 cgcttattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa gtgattgcta
      541 ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg atagaggact
      601 gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa aggcaaatgt
      661 tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa cgctactcta
      721 taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag aaaaaaatag
      781 ataaaaaact aaagaaacgg taaagaaaga cgatttcaat gaaaaatagc aagtaaacga
      841 taacaaccat cctaaatcca caagaaatac agcagacctt gctttaaaaa cacgaatcat
      901 gcaaaggatg aagtaaagaa gacttaacgc gtacccaata ttagtcattt gactattagg
      961 tacgcgtttt gttttataag tcatgacaaa tttccttcgt tgctggatag attttccggc
     1021 attgttgata gattttatga tacttttcta cattttgtaa gagaggttcc actgcatctg
     1081 tatattggat aaaattctga caacaacttt caggtgtgtc gaaccaccct aaccctagtg
     1141 cagcaatcat tgttgcgcct aatgcaggtc cttcctccac acttaaacaa tagaccgttg
     1201 catcgaaaat atcagcttgg atttgtagcc atgcaagatt tttcgcgcct cctcccaccg
     1261 aaacaatacg tgtaaatctg cgacccgcta gttctttacg tttcaatccc accattgaca
     1321 aatgctcatt aaaaatttag aatattcaca agtttaacga caattatgaa aatttatgtc
     1381 gtgctaaagg agttttgtta ttggatgaaa agttagatat aaaaactgcg atcaaagaca
     1441 cgttacctac cgttttcggt tatatcggta ttggacttgc atttggtatc gttgggaaag
     1501 ctgccggatt tcatccacta gtcgtcacgt tgatgtccct gctggtctat gctggttctg
     1561 cccaatttat cacagtcagc atgcttgcta gtcacagccc attgctttcc atcgttttct
     1621 cgacctttct agtcaattcc cgaatgattc tgatgagtat gacgattgct ccttatttca
     1681 agaaaaatcg gctgcttcaa aatctgctga ttggcacatt gctgacagat gaaagtttcg
     1741 cactgggaat gaataaattg aactatactg gacaaaagtt aaactttcgt tggatgaata
     1801 cagctaattg gatttcttat ttgacgtggg tgggttcttc tcttgttggc gcacttttag
     1861 ggaattttat tactgatccg aaaaagtttg gtttggattt cgccatcgtc gctatgttta
     1921 tcgggttact ttatcttcaa gtcatttctg atagaaacac ctctaaacga ttacagttga
     1981 tcctgattgg tctcactttg attctggtat atgtcggact gatttttatc ccaagcaact
     2041 tagtgattgt agttgtaaca ctgattgggt gcggattggg ggtgtggatc aaacatgcct
     2101 tcttttgagt ttacattgct gactatttta ggttgtactt tagcaacttg gctttctagg
     2161 gtcctaccat ttgtcttact gaaaaaattc gatttaccgc aaccgttgct tgagtattta
     2221 agttttgtcc cgatcgtcat tatgtctgct ttatggttca gcagtctatt tacccaaaat
     2281 atcggacatc tgccgcagat caatctcgaa aatgctttgg cttcagttcc tacactttta
     2341 gctgctattc tttcaaaaag cttattggtg atcgtcttgg caggaattct ttccctttcg
     2401 ttgattcgct tattataaat aaaaaaacaa gagcttatgg caaattactg tcatgcgctc
     2461 ttgtttttat caaagtcaaa taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg
     2521 gaccactaac aacgactttt cctaacatat gtcctgtttc tacgtctttg gttgcttgtt
     2581 tcaagttctt cgcattcatc ccatctgaat agaccttgct caatgtactg cggataatcc
     2641 cttcatttgc taaagcagca atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt
     2701 tatagtctgt tttagcgaac atatactccc agtcgaagct gatagattgg tttttccaaa
     2761 cacctatatc taacggctct ttgatcccta cgatcgttcc cacatgtcca aatggtcgga
     2821 caaggtgctt gatttgatca aagtagcggg taatatcgaa taatacagcg acatactcta
     2881 cttgatcgat tcctaattca cgaaaacttt tacttaagtc gtgatgataa tcgattggat
     2941 agtctacacc tgtttttttc agccattcaa aattcttcgg acttgcagtc gcgtagactt
     3001 ctaggccagc ccaatgcgct agttgattca aaatcgaacc gacgcccccg gatccgttga
     3061 tcactaagat ttttttcccg cgattggcat tttcttctgg agttagacca aatttttcaa
     3121 ataacaattc ataagcggtc aatgaggtca acggcaaggc tgcagcttct tcatctgaaa
     3181 gattttttgg tgcaagagcg acaatacgtt cgtcaactag ttggtattct tgattgcttc
     3241 ctgctcttgt tgtacctgcg taaaaaacgc gatcgccgac agaaaatttt tgcacttcgt
     3301 cacctacagc aacaactttc ccgactccat caaaacctaa gatacgtaaa gcatttctta
     3361 tcccgttatc ttggcgaagt ttcgtatcga caggattaac agatactgcg ttgactttta
     3421 ccagaagatc ccgtgaactg ggtacaggta tctgttcttt ttcatcaata aagctttttg
     3481 gatcatcgat cggcaaccct tcataaaaac cgactacctt cattaattcc ttttctgaca
     3541 tacgaattcc tccttagctt catcaagttg gagcatccat acagtgttca tttcaatata
     3601 tatagtatgc tatttgttcc acctttttta ttcatcataa ttattctctt acttaaagta
     3661 aactatttgc acttttttgg ttaaaaaaga ggttgcgaca agacttttgt cacaacctct
     3721 tataaacggt gttctcctac caactcttcg gtcacaagtc a
//
LOCUS       DACENJ010000208         3695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.215, whole genome shotgun sequence.
ACCESSION   DACENJ010000208 DACENJ010000000
VERSION     DACENJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.215"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(189..1337)
                     /gene="nagA"
                     /locus_tag="IW748_002350"
     CDS             complement(189..1337)
                     /gene="nagA"
                     /locus_tag="IW748_002350"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1105248.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1413..2249)
                     /locus_tag="IW748_002351"
     CDS             complement(1413..2249)
                     /locus_tag="IW748_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1105249.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2433..3650)
                     /locus_tag="IW748_002352"
     CDS             complement(2433..3650)
                     /locus_tag="IW748_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1105250.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
BASE COUNT         1070 a          780 c          607 g         1238 t
ORIGIN      
        1 caataaaaaa agcttttatt ttgctaatac gtattaacat ttttaatttt atctttacat
       61 tgttacttat tgaacaatag gaaattaaag aaacttttga aacttatgta aagtgatttt
      121 tactagaaca caaaaaaacc ttaaaaccaa acttaagaag tttagtttta aggttaactt
      181 aaattatatt atgcgtggaa acgttccacg ccatctagat aagttgcttc caatgtcata
      241 tcagggttca atacgataaa gtctgcatcg cgtccttgag cgatttttcc acatgtatca
      301 tcgattttac agctgattgc tggaatcaga ctagccatca tgactgcttg ttctggtgtt
      361 gctaatcccc agtctacaac atttttaatt gcttctttca atttcaatat acttccagct
      421 aagttgccag attctagacg tgctgttcct tcttttacta ctactggaaa ttcgcctaaa
      481 ttgtaatttc catcaggcat cccaccggcc atcatacagt ctgtaattaa tgcgacatga
      541 tcatgtcctg ctttttccat taggattccc gcagcatttg gatgtacatg atggccatca
      601 caaatcaatt cagaaaagac atgttctaaa gataataacg cacccaccat acctggttcg
      661 cgatgattca atccacgcat accattgaaa gcatggacaa atacacttgc gcctgcttca
      721 acagcttttt gcgcttcttc tagtgtagca ttactgtgtc ctagagaaac aactactcct
      781 tcttcagtca cttgtttgac aaactcttct acaccttgac gttccggtgc taaagcaatt
      841 tttttgatta aaccacctga agcttcttgc cattcatgga aagtatccaa atcaggatcc
      901 ccgaaataac ttgggttttg tgcccctttg tgttcttctg tgaagaaagg accttcaaaa
      961 tagattcctt tgattttagc tcctggtgct tcctgataca ttttgccgat tgtctcagct
     1021 acatctttta aacgttcttt gcttgacgtc aatgttgttg gtaaaaatga agtcactcca
     1081 caagataaca gaccttcaga catcactttg atgccttctg catcattatc cattacatca
     1141 tgattcatat agccgtgaat atgtgtatct accaaaccag gagcaatcca tttgccgctt
     1201 tggtcaatga tgttgacttc tccctcaggt agttctttgg tgtattcacc aaaaagtcca
     1261 tcggtaattt caagatagcc tgcatgtttg acacctgacg ctaaaaagaa tttgtcagca
     1321 tagataaaag ttctcattct attctctccc tttgtttttc tacctctaaa gtactcttat
     1381 ttagagaaga agtcaagaat ggtctacacc atttacaaat tttttgaaat ttgtttcaca
     1441 gcttgtgcta agccttcata gaatgctgct ctttctggtt cttctttacg cttgtatatt
     1501 aagcttttaa gcacaaataa atcattgagg taatagtagc tattcttctc ttgagcccaa
     1561 tagattcctt gattcacctg ttctaacgct tcgtcgattt cattttgatg gaataagaaa
     1621 tttccataat tataaaaaat ttgcgaaaat tcactttttg atcgttcatc agctgattga
     1681 tgcagcagtt ggatgcttcg actcaaatag tagcgtgctt cagccgtttt tcctgccaca
     1741 ccatatattt ttccgataca actaatcagt tgtatttctg tgtcagaaac atggattctt
     1801 tctttttggc tagagtagct taatgcttct ttcaaataat aaagggactg agagatattt
     1861 tgacgcaacg tgaattcgca aacaccgaga taataataat agcgttgaaa gtcagttgct
     1921 tgatacagat tttcagaaat atcagaagat tttagtactt gttctaactt ccgataatca
     1981 cgtttttgga aacaactatc caacagttca aataatttat cgtttgtccg agtagttttt
     2041 cgagagagat tcatcacatg atcaatcgtt acccctagcc gttgacaaat ctgcatcatc
     2101 accaatacat tcggcagttc ctcattattt tcgatccgac ttaatacact ttgtgaacaa
     2161 atatcatgcg aaagcatttt ttgcgtcatt ttttgttctt ttcgaatctc tttaatgatc
     2221 gttccaaagg actcgattaa cgtttccata agtatcctcc ccaaaatatg cgtattcgca
     2281 ttctattcgt attttacttt aggaagtcgc aaacttcaag aaaagacttg caactaaata
     2341 atatacttta gttatttaag aaaactccac tagaaagaga gcctcagtgt gtttgtctgt
     2401 ggccctccta ctgaaaaatt tggtttttaa aatcattttt ggcgataggt tgctaggaat
     2461 tctctcattt tcttagctgt ttgtttcaaa tcatccattt taggaagata aacgattcga
     2521 aaatgatctg gctggttcca attaaatcca ccaccatgta caagtagaat atgatgttca
     2581 tgtaaaaagt ccaaaacgaa tttttcatca tccaaaatat tgaatcgctt tgtatcaatt
     2641 tttgggaata tataaaaagc agcttttggt tttacggctg aaagaccagg aatatcattg
     2701 attgcattat agatatattc tctttgttca tagatccttc cgcctggcag gagcagctca
     2761 tcgacacttt gataaccgcc tagcgcggtt tggataattt gctgagacaa tacattggaa
     2821 cacaaacgca tggaggacaa catattcagc ccttctatat agcctttgac gtgtgactta
     2881 tctccactta ggaccatcca gcctactcga aatcctgcta cacgatgaga tttggataga
     2941 ccgttcagcg taacaacgaa caaatcaggc gctaatgtag caatcggaat atgttctaga
     3001 ccatccatca ctaagcgatc atagatttcg tcggaataaa taatcaggtt attttgtcga
     3061 gctacttcaa cgatttcttc taataattct tttggataaa gtgccccagt aggattatta
     3121 ggattaatga taacaatagc ttttgtccga ctcgttattt ttgatttgat atcatcaata
     3181 tcaggatacc attctgcctc ttcatcgcaa atataatgaa caggtgtgcc tccagctaat
     3241 gcaatagaag ccgtccacaa cggatagtcc ggcattggaa ccagtacttc atctccattg
     3301 tccagcaacc cctgcataca catcgtgatc aattcgctca ctccgttgcc ggtgtagata
     3361 tcattgatcg tcacattcgg aaaatgtttc agttggcaat attgctcaat tgcttttcta
     3421 gcagaaaaga tccccttgga atctgagtag ccttctgagt cacgggcatt catgatcaaa
     3481 tcacgaatga cttcgtttgg tgcttcaaat ccaaaaggtg cgggatttcc cgtgttcaat
     3541 tttaaaatac taatgccctc ttcttgcatc cgctccgctt cttctagaac gggaccgcga
     3601 acatcgtaac tgacaccttc taatttgttg gatttttcaa attctctcat cacttagcca
     3661 atccctcatt tcaaaaagtt agaactttcc aaatt
//
LOCUS       DACENJ010000209         3604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.216, whole genome shotgun sequence.
ACCESSION   DACENJ010000209 DACENJ010000000
VERSION     DACENJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.216"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            438..1043
                     /locus_tag="IW748_002353"
     CDS             438..1043
                     /locus_tag="IW748_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HAQ1105251.1"
                     /translation="MILENKLGLTNQVELAKAEEKLSKQKAKELYDSGKINEIEVGTF
                     KGLSEIHDFLFSEIYDFAGKIRTVNIAKGNFRFAPVMYLEQSLNHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1059..1634
                     /locus_tag="IW748_002354"
     CDS             1059..1634
                     /locus_tag="IW748_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1105252.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKLFAKKNNPNFKEGRPKATITPKKRHAYELI
                     TSGKSYKEVEAITGFSRSTLFRIKKQIEASE"
     gene            1794..1997
                     /locus_tag="IW748_002355"
     CDS             1794..1997
                     /locus_tag="IW748_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1105253.1"
                     /translation="MKEQQLGYTLWGRPLYGLERRTAVSIDCACGELIQLNIQTKQYN
                     CKNCGNCYQFIDQDFQLLNEKNK"
     gene            2106..3431
                     /locus_tag="IW748_002356"
     CDS             2106..3431
                     /locus_tag="IW748_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1105254.1"
                     /translation="MLDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKNLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIAPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKI
                     SITDNTRELQDYCLFLFNKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            3424..>3604
                     /locus_tag="IW748_002357"
     CDS             3424..>3604
                     /locus_tag="IW748_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105255.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANQNLAKEQMRTE
                     EIAEILNASVIKNRSV"
BASE COUNT         1304 a          560 c          737 g         1003 t
ORIGIN      
        1 atcacaaaca caaacagaaa aataaaaaaa tcaaaaatct aataatcata aattgctacc
       61 gaagcacctt ttattataat taatcaaata taaaattaca agtggttttt ttaattatta
      121 ctaatactca aaataaaaac agggtatgct actgaaaaat aaagaaacac ctgattttat
      181 gaacactttg ttttaaaata agaactgaaa aaatcactca aaataattga gtgattttct
      241 tagcgattaa atgtttatag tttgtgccga ataaaataaa aaatttttgc catttaaaaa
      301 gaaaaggaca gatggcttct attgaaaact aactacgtgt caaaaactta atagtttttg
      361 acacatggtt aaattcgcga tacaatagga tttgtagtgt ttcataaact tatgtcaaaa
      421 agttaagggg attttccatg attttagaaa ataaattggg tttaacgaat caggttgagt
      481 tggccaaggc tgaagaaaaa ctcagtaagc aaaaagcaaa agaattatat gattctggaa
      541 aaataaatga aatcgaagtc ggcacgttta aaggcttgtc tgaaattcat gattttctct
      601 tttcagaaat ttatgatttt gcaggaaaaa ttcggacagt taatattgca aaaggaaatt
      661 ttcgttttgc gccggtcatg tacctagaac aatctttaaa tcatattgat cagatgccgc
      721 aaacttcttt cgaagagatt attaagaaat acgtggaaat gaatatcgct cacccttttc
      781 gcgaaggaaa cgggcgcagt actagaattt ggctggatct cattttaaag gaagaattgc
      841 aaaaagtggt cgattggaac ttgatcgata aagcagacta tctttctgca atggagcgca
      901 gtccaataaa tgacttggaa atacgctatc tcatttccaa tgccctgact gataaaattg
      961 acgatcgtga gttatatatg aaaggaatcg atgtcagcta cttctatgaa ggatactctg
     1021 agtatacgat agatgatttg taaaagcagg tgagttaaat gacaaaatat ggctatgcac
     1081 gtgtctcaac cgcttctcaa agtacaaagg aacagataca acagctactt gagaacggtg
     1141 ttgagaagaa gaatattttt tctgaaaaat ttacaggtac gaaacgagat cggccaaagt
     1201 ttaatcagct gttggctggt ctgaagcctg gagacgaaat cgttgttacg aaacttgatc
     1261 gattcgcccg taatacaaga gaagcactag agatcattga acctttactc gatgataacg
     1321 tcacgattaa ggtattgaat ctagggacga tcgaaaatac atcgatgggt cgaatggtta
     1381 cacgtacgtt gttgagtgta gctgaaatgg aacgtgatat gattgtagaa cgtactcagg
     1441 aagggaaatt atttgcgaaa aagaataatc ctaattttaa agagggacgt ccgaaagcaa
     1501 caatcacacc aaagaagagg catgcatatg aattgattac ttccggcaaa tcgtataaag
     1561 aagtggaagc cataacgggt ttcagtcgta gtacactttt tagaattaag aaacaaatag
     1621 aagcgagtga gtagaagatt gataaaatta atataaaaat ctcttttaaa tcaaataaat
     1681 cgttgtttat acgagcattc taaggtcagg ttcactcatt aagatgtatc attggtcgaa
     1741 cgaagcgtca taccagctac gaatagacat atttttaggg aggaacgaag taaatgaaag
     1801 aacaacaatt aggctacact ctttggggac gtccattata cggattagaa cgaagaacgg
     1861 ctgtatcgat tgactgtgct tgtggtgagt tgattcaact caatattcaa acgaaacaat
     1921 acaattgtaa aaattgtggg aattgttacc aattcattga tcaagacttc caattgctaa
     1981 acgaaaaaaa caaataggtt tattatagtc agacaattgt ctggctattt ttctatttag
     2041 agaagatcct cgttgcaaac acgaacgtat gtttgtataa tatgagagaa gagggggaga
     2101 actttatgct tgattactca aaagagccag tcaatgacta ctttctaatc gatatgaaaa
     2161 gtttttacgc aagtgtcgaa tgtatagaaa gaaatttaga tccattaacg actgaattag
     2221 ttgtcatgag tcgagcggat aatacaggat cagggttgat tttagcttct tcaccagctg
     2281 caaagaagaa atatggtatt tctaatgtca gcagaccacg ggatctacca cagcctttcc
     2341 ctaaaacatt acatgttgtt ccaccacgta tgaaccttta tattaagcgt aatatgcagg
     2401 taaataatat tttcagaaga tatgtggctg atgaagatct actgatttac tcgatcgatg
     2461 aatcaatctt aaaagtgacc cgatcactga atctttttac gactgaagga acacgaagtc
     2521 agcgaaggaa gaatctcgct caaatgatcc aagaacgtat taaagaagag ctaggattga
     2581 ttgctacagt aggtgttggc gataatcctc ttttggctaa actagcctta gacaatgaag
     2641 cgaagcataa tgaaggattt attgccgaat ggacgtatca aaatgttcct gataaagtat
     2701 ggaatattcc tgaaatgaca gacttttggg gtattggttc tcgaatgaaa aaaaggctca
     2761 accaaatggg gatcatgagt atcaaagatt tagctaattg gaatccatac accattaaga
     2821 gccgtttagg tgtgattggg ttacagctgt acttccatgc aaatggtatt gatcgaactg
     2881 acatagcaat cgcaccagaa ccgacaaagg aaaaatcata tggcaattca caagtgctac
     2941 cacgagatta tactagaaga aatgaaatcg aactggtcgt aaaagaaatg gctgagcaag
     3001 tagcaatccg tattaggcaa caccactgta aaacaggttg tgttcattta cacattggaa
     3061 cgtcgatttt agaaacgaga ccagggtttt cacaccagat gaagatatct atcacagata
     3121 atacaagaga gctacaagac tattgtctgt tcttatttaa taaatactat gagggacaag
     3181 aagtccgcca tgtcggaatc acttacagca agctagttta tactgattcg cttcagctgg
     3241 atttgtttag tgatccacaa aaacagattg atgacgaaaa actagacaaa ataatagata
     3301 aaattaggca gaagtatggt tttacgtcaa ttgtacatgc ttcttcgatg ttagaaggcg
     3361 ctcgtagtat tactagaagc acgttggttg gtggccatgc aggcggcgca ggggggctta
     3421 aaaatgattg actataacga tcgaggacga ttgaaatggg caggtttcta tttatcggat
     3481 catactgaag agatcgatgc agataatgag caacgtgcaa atcaaaattt agcaaaggaa
     3541 caaatgagga ctgaagagat tgcggaaata ttgaacgctt cagtcataaa aaatagatca
     3601 gtat
//
LOCUS       DACENJ010000210         3584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.217, whole genome shotgun sequence.
ACCESSION   DACENJ010000210 DACENJ010000000
VERSION     DACENJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.217"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            189..2333
                     /locus_tag="IW748_002358"
     CDS             189..2333
                     /locus_tag="IW748_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1105256.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2385..3425)
                     /locus_tag="IW748_002359"
     CDS             complement(2385..3425)
                     /locus_tag="IW748_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1105257.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         1155 a          761 c          663 g         1005 t
ORIGIN      
        1 tgcaataaaa tgaacgtcaa agaaaaacga atgaatgctc gttgcttatt tacaaattgt
       61 tttatttaga atacacagac agatttatga aaagaaaaag cttttcgaca cgattatctg
      121 ttccaaaaaa tgtctaatga atgctgaata agacagaaaa tcaatgaaag gaaaaggact
      181 agtttattat gaaaaataaa aaaacgatac gtttgctagc tgctacttgg ctgattgctc
      241 cgtctgtttt gtcgaccaat accgtatttg ccgtagataa tcaaatagct gatcaaacag
      301 tcacaacaga tacgactaca tccagctcag ctactagtga aacaacggat tcttttacag
      361 aagacccaac tggaacaact aactcaacaa caggatcgac cgagccaact gaaattgatc
      421 cggctgatcc atcagaaaat gaaaagccag ccgagacata ttcccttaca attgctcctt
      481 ctttgattgg taagatccaa ctgactgctt cgacagcgga ttcccctgaa cagcaacctt
      541 tggatattac ggaaagtgga acggtagaag gtttattagc ggaaacgaag gtaacttaca
      601 ctattacgcc agctgaaggt gaacaaattg cttcagtcgc tgttaatggt gccaaagaag
      661 taaattatga tgcaaagacc tttacagtag gtacgaacaa tttatctatt actgcagaat
      721 ttaaagctgg accggatact tcaacagaga aaccaactga tcctagtgaa gaagtcccta
      781 gtacaccaga tccggaaacg ccaaacaaac cagatgtggg taataacaac aataataatg
      841 gttcaaataa ttcaaacaat aataacaata attcgaacag cgggaattct tcgaatcaaa
      901 caaacccatc tggatcgaat aacaatggaa atactcagag agaaccaaac catccaaaca
      961 atggttcatc gaatggcgga acgaacaatg cttcatctcg tccaaataca gcaaatcaaa
     1021 gcggtataga gaatccatct tctgcttctt ctgattttgt tgtaaaatca ccaattgatg
     1081 ctgtattgcc tacaaataca acgaatgtcc aacaagcaat cgttcgagaa gcattcaagc
     1141 atcttggaaa accatatgta tggggagcaa aaggtccaaa tacttttgac tgttctggtt
     1201 tgacttatta cgtatatatg aaagcaaccg gtcattatat cggtggctgg acaggcgaac
     1261 agcaatatgc aggtacgcag attccagtaa gtcaggccca acctggcgac ctcgtcttct
     1321 ggggaccttc ttccggtgtt actcaccatg taggtatcta tattgggaac ggacaattta
     1381 tccatgcgcc tcaaccagga gataaagttc gagtaacttc tatttcagat ttcacgcctg
     1441 attttgctgt ccgagtaaac ttagcaggtt tacctgctgc ttcaggttcg ctgacaggcg
     1501 gctcatctat tttagatgga ttagatggct cgttccattt cagtcaaaac caaacaactg
     1561 atcagttctt gaaaaagatt gcagatgatg cacaggaaat cgggcaaaaa gaaggaatct
     1621 acgcatcagt catgatggct caagctatcc ttgaaagtgg atctggaaac agcttattat
     1681 caagcgaacc aaatcataac ctttttggta tcaaaggatc atataaagga agcagcgtga
     1741 ccttcaatac acttgaacaa gatagttccg gacaaagcta ccaaatccgc gcacaattcc
     1801 gtaaatatcc ttcttacaag gaaagtttgg aagactatgc cgacttgatc aaaaatggac
     1861 tgactggcaa tcctgatttc tacaaaccaa cttggaagtc ggaaacaaaa gactacaaag
     1921 aagcaaccaa atatttggaa ggacgttatg ctacagatcg ccaatattca caaaaattga
     1981 acgcgatcat cgaagcgtac gacctgacaa aatatgatga accgaaaaaa gaagcagcaa
     2041 caactgaaga agcaaacgct gaaatcctca atatgtctaa gcgattcgat gtgccaatca
     2101 aatggacaac agatggcgtg ccaatcacag gattagaatt ttcaacgata aaacgcgtca
     2161 acaacgcttc ttccatcttc cgtgtcgctt caatctggga tttgtggaac aattttacgg
     2221 caagacagat accagaaaca actactcgta cagttaaagc tgcaactgtt cctgtactaa
     2281 ccttgttaag tttagacaaa tcattctggc ttgatctgcc tttaaagaaa tagaataaat
     2341 taaaaagggg atgtgacact gtcacactcc ctttttaatt tattctattt ctttatattt
     2401 attttgtatt ccgttacaaa ctcgcgtact tggttaggtt ccagagtgac atctccaaaa
     2461 ctttcaagtc gctccgctcc tggcagttct tgagtctcaa atgtgatacc gccatgattt
     2521 actaatttat tcccgcgcat ttccggtcca tcttctccaa aatttgctgt aaaaatcacg
     2581 attcctgaag catctgtttt tacttggatc gatacttttt catctgggct tgtcaattcc
     2641 gcagcattta gtaaaccaga ttgttttttc aacaaaaatg gatgatcgat gccgcccact
     2701 atcattgatt ggcgaaaatc actggcaaag acctcaccta attttttagg acgttggaag
     2761 tcaaatgctg tgccacttac tgaaactact tctcctgtag gcgttgtatc tttattgagc
     2821 gtagcaaata atcggctatt caaccaaagt atatgttcgt cgattgactg tgctggatca
     2881 ccggttaaat tgaaatagac atgatttgta ggattgaata ctgttttgcg gtcactgatc
     2941 ccttggaccg tcactcgcca tgtatcatcg ttcgtcagcg tataccgtac ttctacatct
     3001 aaagttccag gaaatccgtt ttccatatct gggctagtct gatagaaaat gaccgatgct
     3061 tcgttttcac cattgatgac tgtataatgc cactgcttgg cttcaaagct aggtgcacca
     3121 ccatgtaaac aatgaccagt gtccgggtct gtttttactt cgaacgtttc atcatctaat
     3181 ttgaatgtgc cattcgtaat ccgtccagcc atacgtccga ttgaagcacc gatataaggg
     3241 tctttttcta gatattcttt tgcttcatca aaacctaaga tcaactctcg tttttcatta
     3301 tcgattattg tttgaaaatg aacaacccgt gccccaagat cactgacaga caaagatgtc
     3361 ccattttgat tcgtgatcgt aatcaagtga tagttttcgc caaaattttg ttcattgatt
     3421 tccatgttcc ttgcaccctc ttttctttca tttgcactga ttccagcata catggaaacc
     3481 gttttcttaa cagcttattc cgtgagtaac gagaaaaaat ttactatatt ctaataaaag
     3541 accgtgaaaa aaaggttgtg acaaaaatat tgtcacaacc tctt
//
LOCUS       DACENJ010000211         3580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.218, whole genome shotgun sequence.
ACCESSION   DACENJ010000211 DACENJ010000000
VERSION     DACENJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.218"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..894
                     /locus_tag="IW748_002360"
     CDS             4..894
                     /locus_tag="IW748_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1105258.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(967..1791)
                     /gene="nadE"
                     /locus_tag="IW748_002361"
     CDS             complement(967..1791)
                     /gene="nadE"
                     /locus_tag="IW748_002361"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1105259.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1803..3275)
                     /locus_tag="IW748_002362"
     CDS             complement(1803..3275)
                     /locus_tag="IW748_002362"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1105260.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         1021 a          748 c          637 g         1174 t
ORIGIN      
        1 tatatgcaac tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa
       61 acaaggatca agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca
      121 aaagaacttg gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca
      181 aacatcatca aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa
      241 atcgatatca gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata
      301 cctattggtc actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat
      361 gattttatcg ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc
      421 cgcattctct ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca
      481 gaggatacat tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca
      541 aatgacaatt ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg
      601 acgagccctg gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat
      661 aatcttaatc aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg
      721 aatggagaac ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg
      781 catttgcgaa tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt
      841 aaaggattcg gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt
      901 tcttttctat tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct
      961 ttattgttat ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt
     1021 cttgttccac catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag
     1081 ataatcatca atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt
     1141 cccatcttca agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc
     1201 ttgaagcaat tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc
     1261 accatcacca aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa
     1321 tacggctcct gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt
     1381 gatatttcct ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg
     1441 tgcatcaaca gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag
     1501 agcagctttt gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa
     1561 ttgatagttt tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg
     1621 gcctgctaaa gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg
     1681 atattttttc aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc
     1741 aattgttggt ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc
     1801 tcctatgctt ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt
     1861 ttatggttcc agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt
     1921 ttatactctt cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt
     1981 gcaggtaatt cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct
     2041 gtaaagtctc gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct
     2101 tcttcttctc gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca
     2161 aaatttcgag tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg
     2221 cttgataact tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg
     2281 aatactgcac caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat
     2341 acatcaattt tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc
     2401 gaagcataga tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta
     2461 gatatatagg ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct
     2521 aattctttcg ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg
     2581 actaagaaca cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt
     2641 ccataagatt ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc
     2701 ccagcacgta cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag
     2761 actgctgcat ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg
     2821 atgacagatt tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc
     2881 aacagtgctg tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt
     2941 tcatttgcaa aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa
     3001 ttttgtaaat actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata
     3061 tcggaatcat tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa
     3121 accgcgtatc cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa
     3181 tcagctcgac ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc
     3241 agcgtcaaac tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt
     3301 ttcatttcct acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc
     3361 tgaaaaagca catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact
     3421 atcctttctt attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc
     3481 cgagacacga agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc
     3541 ctactacaat cgtagccgtt aagataccga tgattgaaca
//
LOCUS       DACENJ010000212         3557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.219, whole genome shotgun sequence.
ACCESSION   DACENJ010000212 DACENJ010000000
VERSION     DACENJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.219"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            159..3437
                     /locus_tag="IW748_002363"
     CDS             159..3437
                     /locus_tag="IW748_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1105261.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         1243 a          636 c          772 g          906 t
ORIGIN      
        1 tttttacgtt aataaatctc tttattatca acaacttaag ttatattttt tctttttata
       61 aaatacactt attataattt tgttttttat tgacagtaaa aataaattag gtaatacttt
      121 aagtaaacgc tttctaattt gttacgggag gaatacactt gttaaaaaaa cgtataaaat
      181 acagtgcgct aatgatgaat ctccttatgc tcagtacccc ggttttaatt tctgtcgttg
      241 aagttgctgc taacgaacaa cagtctgtga acgaagaaaa tgcacacgtg aacattgaag
      301 ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga tatggatacg gaagaaggaa
      361 atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa aaagcaatct gaagaagcaa
      421 atgaagagtg gaaaggggtt gttttcggag aaagcacaag tgctagtagg tatgccattg
      481 aaccattaga agacggcgtg cggctctctt ccacgaataa cggcggaaaa ttccagtcaa
      541 gcggatcaga tggaatgacc tattattatc aagcaatccc caaagacatc aactttagga
      601 tgcgggcgac gatcgaaata gaatcttgga cctatacgaa tgcccaagaa ggatttgctt
      661 taatggtaag agacgcagta ccaaaaaatc atttgtttgg gcaggcattt tattcaaatt
      721 catttgccat actaggcagt cggatcgaat atgtctggga ctcggataga caagaagtgg
      781 tcaacacgag cggcagcaaa tatacgatgc gacttggatt ggggacacgg gcaatcacag
      841 gaatatcatc tgaagatccg caagctgcgc cagctcctgg gagtgtctca gtggaaacaa
      901 cacctttaga gacttcagca gggttcttgg aaaaagaaac aggttcttat aatatattcg
      961 gaaacggtca tgggaatctg tttccggcca cgaactcgat tactaaactg gacgtagaga
     1021 tgaaaaaaac gaatactggt ttagaggcat attattacga tcctgaaaca ggagaagaaa
     1081 tggcttctga catcatgtat gactgggaaa aattatacgc ttcagatgaa tcggttattt
     1141 atgcaggatt tgctgcagca aggaatatga cgatcaaggt agaaggaatc gaagttgctt
     1201 actcagatcc tgcgacagat cctcctgggc aagaaagacc tactcgcttg attgatccga
     1261 tttacactgt gacctctagt aatcattcag gaaaccaaga tcatactatg tcctttagag
     1321 caaatgctga cggattattg acaattaaaa ataaagcaac aggagaggtg cttaagaacg
     1381 caaaaaactt ggctatcact gcgaatcggg aatttgatta tcagacaaaa ctgatggaag
     1441 gaacgaacga atttactttc agttttacgc cagataaaaa ctatccgcca gaagaatacg
     1501 tagagatgac tagttatgaa acaaaagaga ttcttcatac agtggactac cgtaaaccta
     1561 aatattcgac agtttatgta acaccagaag ggtcagatgc aggaaacgga agcagacaaa
     1621 atccattatc tctgacacaa gcattgagat acgcatatgc tgggcagaca atcgtcatgg
     1681 cgcctggaac ttattccttc gaaagaggat tgacgattcc aaaaggcgtt aacgggacaa
     1741 gagaaaaccc gattcagttg attgcagaga aaaatccaga aaacaagcgt cctgtacttg
     1801 attttaaagg aactggaaat gggatgacac tgttcagtca ctactggggt ttacgagggt
     1861 ttgacattac aaatagtgca gctatgcaaa aaggattaca gatttctgga aatcataatt
     1921 tgatcgaaga gattcatgct tatcggaatg gaaatacagg aatccaaatc agcggtagct
     1981 caaatgatcc tttcgaagca tggcccgcgc ataacttagt caagaactgt acatcttttg
     2041 aaaatgccga tccaggattt gaagatgccg atggatttgc ggccaaactg acaataggag
     2101 aaggaaatgt atttgacggt tgtatcgcgt atcataatgc agatgacggt tgggaccttt
     2161 ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat caaaaactca gttgcttatc
     2221 gaaacggctg ggtaccagga attgagggag aaggtaacgg taatggattc aaaatgggcg
     2281 gttcttcttt aacgggccct catgaattga tcaatagttt gtctttcgaa aatcttgcga
     2341 aaggaatcga ttccaatagc ggaacagata ttatcgtcaa tagcagtaca agttttaata
     2401 acggttcata taatgtggct ctttatacct cttcagcagg aaacacagac taccatgctt
     2461 ctggtatctt atctttccga acagagaatc ttgagatgag agagcagatc cgcccattga
     2521 atcaaaacga agatcaaatc taccactcgt tgaactacta ttggaatgaa cgagagaaac
     2581 aatcagaaaa tactcttgga cataccatat caaaagattg gatcgagtct ctaaatacat
     2641 gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa tggaagtatc catgttcatg
     2701 gactgatgca gattaaaccg gggaatcgtc aaacagaaaa ggaaagagtg gatggtcttc
     2761 cattatctgt aggtgctatt tttgacggag aaacctcacc gtcttcggaa tatccagagt
     2821 atgcaatcgt acaagaacca aatcagacaa aaccaaatga acaaaataat ccgattatta
     2881 tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa tatcattgaa ctgaagcctt
     2941 ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac gcctgtcttt gaagatatgc
     3001 gttatttccc agaaaagatc caaatcgttg aaacacagtc aagtgctcaa ttttctaatc
     3061 ataccgctat tttttcagca ccttcaaaag gagaatatac gctagaagtc acttatcgtt
     3121 cggaaatatt tgatggggaa aaatggtctg ctcatacaga atctgttacc cagcaaaaag
     3181 cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa tgacaaacca gaaagagaaa
     3241 atccaggaaa tgaatcacca aatggaaaac caaatggaaa accttctcat ccggatagga
     3301 cagcaaaaga aagtgaaaaa aatggcactc aaggactccc aaaaacggga gaatttaata
     3361 acccgttgct agcattagct ggaggaattt tgttaatcgg tgtagttgtt tatgtcatga
     3421 aacagcgaaa aaaataagtg gaatttctcc ttttatgatg taaaacaatt gactgaggaa
     3481 agtaaaaata gaaggaaatg agaccaatag atgtctcact tccttctatc tttttataga
     3541 tgaggttctc cttgctg
//
LOCUS       DACENJ010000213         3524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.220, whole genome shotgun sequence.
ACCESSION   DACENJ010000213 DACENJ010000000
VERSION     DACENJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.220"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            214..999
                     /locus_tag="IW748_002364"
     CDS             214..999
                     /locus_tag="IW748_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1105262.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1012..1863
                     /locus_tag="IW748_002365"
     CDS             1012..1863
                     /locus_tag="IW748_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1105263.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(1987..2439)
                     /locus_tag="IW748_002366"
     CDS             complement(1987..2439)
                     /locus_tag="IW748_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1105264.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(2904..3272)
                     /locus_tag="IW748_002367"
     CDS             complement(2904..3272)
                     /locus_tag="IW748_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1105265.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3422..>3524
                     /locus_tag="IW748_002368"
     CDS             3422..>3524
                     /locus_tag="IW748_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1105266.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKK"
BASE COUNT         1144 a          567 c          689 g         1124 t
ORIGIN      
        1 aatttttgta aattacttct tatttcttga agctggccac ttctgtcaca acctctttct
       61 ttatagaaat aaaaggacac tagcaattca tgaaaacgat tgctagagcg tatcatttgc
      121 tttagactat atatcttggt gtacactcac tttaagacgg aaataaaagt tatccgtaat
      181 aacaggttta ggtatgaaaa aggagtgggt actatgtata aggaattaga aaacaaagtc
      241 gcagtagtaa ctggtggctc aaaggggatt ggaacagcga ttgctcaacg attcgggaaa
      301 gaaaaaatga atgtagttgt caactataac tcggataaag aaggagcaga aaaagcagtc
      361 aaagcaatcg aagaggcagg cggaaaagca gtctctgtac aagcagatgt cagttctgaa
      421 gaaggaatac agcgtttact tgatacggct ttagaaaatt ttgggacact ggacgtttgg
      481 attaataatg ctgggatgga aaatcagcat ccaacccatg aacttagcct tgaagattgg
      541 gaaagagtat taaaagtcaa tttgactggc gtttttatcg gaacgaaagc ggctttaaat
      601 tactttacag agaatgataa aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa
      661 atcccatggc caacgtttgc tcattacgct gcttcaaaag gcggagtaaa actattcacc
      721 gaaacggtag caatggagta cgcaccaaag ggaatcagaa tcaatagtat aggacctggt
      781 gctatccgta cacctattaa tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg
      841 gaaagtatga tcccactagg acgcgtaggt gaaccaaaag aaatagcggc gacagctgca
      901 tggttggctt cagacgaatc cagttatgtt acaggtatta cactatttgc agatggtggg
      961 atgactttat atccttcttt ccaaggtgga aaaggataag ggtgaataaa aatgggttta
     1021 ttgattgcat tgatcccggc agttgggtgg gggatccagc cactgatttt gaaaaagatt
     1081 ggtggcagac caacaaatga gatattagga actggtattg gagccgtgct cgttggctta
     1141 cttgttcaat tattcgcttc tccagctggt atttcagtga ctacattttt gattagttta
     1201 ctttctggtg cattctgggt catcggacaa acaggacagt atacatcttt caacttgatt
     1261 ggtgtttcta aaacgatgcc aatttcgact gcattacagt tagtcggaac ctcattgatt
     1321 ggtgtcttcg cttttggaga atggtctgga agtatgggaa aaatcattgg tgcagcagca
     1381 atcattcttc ttgtatttgg ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa
     1441 ggcggtatca caaaaggaat cagtctatta gcatcaacaa gtattggtta ttgggtttac
     1501 agtgcattgc ccaaactagt tgatgcaagc ggactttcca ttttctttcc gcaaatgatc
     1561 ggtgtctttt taggggcagt gatttatgtg attttccgaa aaccaaccgc attttctgaa
     1621 gacaagagtt ggaaagcaac aatagttgga gccatcttca gtgtgtcagc attggcttat
     1681 attttttcag caaatgaaaa tggggttgct actgcatata tcattacaca gctaaacgta
     1741 gttatctcta cattaggcgg tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa
     1801 aaattaacga ttatcggatt attgctgatc gtagtaggaa gcatggtcac ggtctttatt
     1861 tgaggataaa gatggtaaaa tctcatttat taaaaaaata tttgtttgaa ccaaacagta
     1921 agtacttata aaaaaatagc gctagaattt ccaacatctc tggaatttct agcgcttttt
     1981 aattgtttaa gatacaagtg aaatatctgt ctgcatcaat aatttttcat tgatccattg
     2041 actaaagttt acgacataat tgaagttacc accgattagt atctccgcat gattgtcttt
     2101 ataaagatcg tattgtaaaa gacggtcttt tttatttgcg taaacatcgt tatggtactg
     2161 gactaattgt tctcggaatt gattgattgc tgtaaaatca cgatactgaa ggttgaaacg
     2221 catcgtgatt ggtgcattta gcatgaatcg ctgcatttct tttgctgctt cctctgcttg
     2281 atctccgtat aggaactcaa aaagattacg ttcagaaaat ttccattgcc cgccaacctt
     2341 tgttcctttt agttttcctt catgaagata atttctgata gaacgtgtag taacacctaa
     2401 ttgttcggct aattcattta ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat
     2461 ggttctactg acttagttga tatttgctta atcacgaaat gtagaaagaa cgattttaat
     2521 taaggtcatt gtaaaaatca agtcagaaaa ttctaacttt tctttctcta aagaaattcg
     2581 ttctctccta aagtgatatt atatttaaat atacctgaaa ataatgggca gtattatgaa
     2641 agatcactga tattttctcg tattttttcc ttattcaaaa gttttgcagg aaaattgctg
     2701 aaaaaataat tttactaagt ggaaaattca aagccattca atcagtgatt tttttcagca
     2761 caagaaagac tccttataaa aatagcagat actttgtcaa ttaagtgttt aaccagggat
     2821 gataaaaagg ataggattga acaaaacctg tataagctga tgaatagttt cctcgccagt
     2881 aaaaggttta ttcgtataaa gctttatcgc caaaatttat catgaccttt tttatcttca
     2941 atattaaatg aaatcaattg ttcaatcaat tcccagttga ttacactttt ccaaggaact
     3001 ttgatgacgt tatctgtata tacgtagcct gcttcttgga tatttttttc aagggcacga
     3061 atcgctgctt tttccggtgc gatagaaaaa tgatttttag caaaactaaa tgcaataata
     3121 aaggtaccat ggtcagtaaa cataggttgg ttccatttta cagttgtgtc tagctgggga
     3181 tacgtttctg ttatcttagc aaaaaggtct ttgatctttt gttgatgttc tttttctttg
     3241 atctgattga tgatcggctg aaatttttcc attctaaaac gcctccatta agtggtttga
     3301 tttattttac cagaaaaaca atagttatgt ataaaaaacg aacaaaacac aagtaatgtt
     3361 gtaaccgcta tatttatatg ataagatgtc tcaaataata aaatagaaaa gagacataaa
     3421 aatgattgac ttggcaaaga aattcttaca cgaaaatata tattttgagt tgtatcatca
     3481 tcgagccatt tatacaaatg aagatgcttt ggtaataaag aaag
//
LOCUS       DACENJ010000214         3504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.221, whole genome shotgun sequence.
ACCESSION   DACENJ010000214 DACENJ010000000
VERSION     DACENJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.221"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(14..535)
                     /locus_tag="IW748_002369"
     CDS             complement(14..535)
                     /locus_tag="IW748_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105267.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            784..2406
                     /locus_tag="IW748_002370"
     CDS             784..2406
                     /locus_tag="IW748_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1105268.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            complement(2535..3398)
                     /locus_tag="IW748_002371"
     CDS             complement(2535..3398)
                     /locus_tag="IW748_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1105269.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
BASE COUNT         1147 a          679 c          607 g         1071 t
ORIGIN      
        1 tgtttgattt tacttatcta aatgcataga cgatatgtgt gataccaaat aacataaaat
       61 agaatccaac taaaaagctt aatgtcaacg cagaagaaat tggattaaac aataagaaaa
      121 ttcctaataa aattcctaaa atattgacga tgatcgtaaa ccaataatat ccttcactta
      181 ctcctcttgc caagtcagca gtaaataacg ctaaaactga atcagcaata aaccatactg
      241 caaaaacaaa aggcaatgct gcgactcctg caccaatatt gaataagaaa aagacgccaa
      301 ctagaatatc gatgatcccg attattaagg gcatctttcc tttatatcct gttagctctt
      361 ttaatctgtt ccgtacaaac agttcaaata tccctttaat aaaggcaaaa atcgcaaaga
      421 ctactacaat tgcaactaaa tttccgacag ggtcttgaaa tgataacaat gagacaagta
      481 caaataaaat tcccaaaatc aatgagcccc agtcgatggt tcttttttca tccattttca
      541 atccttcttt catttctttt acagttttat agtactccct cttataaata acgtcaaaaa
      601 ttatgctgtt gcaaactaat aaatcctata gtagctattt tcgctccgct tttattttta
      661 tttttttcat tctgtttcac gtgaaacaca aagaatgttc gattgccttg aatcttttac
      721 ctttccccta tataattcta taagacttgt caaaactaat aatgataaaa gtgaggaaaa
      781 aaattgatta ctgtaaatga tgtgagtttg cacttttcag accgcaaact atttgatgat
      841 gtgaatatta aatttacccc cggaaactgc tatggcttga ttggtgcaaa tggtgcaggt
      901 aaatcaacgt tccttaaagt gttatcaggg gatctgcagc cttccacagg ttccgttact
      961 atgggaccag atgaacggat ggccgtattg aaacagaacc attacgacta tgaagactat
     1021 actgtacttg aaacagtcat catgggacac aagcgtctgt atgaagtgct gaaagaaaaa
     1081 gatgcgattt acatgaaaga ggatttcact gacgaagatg gtattcgagc agctgaatta
     1141 gaaggagaat ttgctgagct taatggatgg gaagctgaac cagaagcagc tgttttactg
     1201 caaggtctga acatccctga agaattgcac catcagcaaa tgagtgaatt aactggtgga
     1261 caaaaggtaa aagtgttatt agcacaaaca ctatttggta aacctgatgt tcttttgcta
     1321 gacgaaccaa caaacggtct ggatatccaa tcgatcaatt ggctggaaga atttttgatc
     1381 aattttgaaa atactgtgat cgttgtatcc catgaccgtc actttttgaa caaagtatgt
     1441 acacatatgg cggatctaga ttttggtaaa atcaaacttt atgtaggaaa ttatgatttt
     1501 tggttggaat ctagtcaact agcggctaaa cttcaagcaa acgcaaatgc caaaaaagaa
     1561 gaacaaatca aagaactcca agaatttatt gcccgcttta gtgcgaatgc atcaaaatca
     1621 aaacaagcaa cttcacgtaa aaaaatgctt gaaaaaatta ctttagacga tatccaacct
     1681 tcttctagac gttatccatt cgttggattc aaacctgaac gtgagattgg aaacgactta
     1741 ttgcaggtag aaaatgtaag tgtaacgatt gatggaaaga aaattttaga tgatatatct
     1801 tttaatttga gcaaagaaga caaggtcgct ttcgtcgcag aaaatgatat aacaacaaca
     1861 acgctattca aagttatcat gggtgaaatt acgccagata ctggaacagt acgttggggt
     1921 gtgacaacaa gtaattctta tttaccaaag gatacaacga aagaattcga tactgatcta
     1981 acgattttag actggttgcg tcaatatgca agtaaagaag aagatgataa caccttctta
     2041 agaagcttct taggaagaat gttgttttct ggtgaagaag tattgaaacc agtgaatgtt
     2101 ttatctggtg gagagaaagt ccgttgcatg ctttcaaaaa tgatgctttc gaaagctaac
     2161 gtacttgtat tagatgatcc aacgaaccat ttagacttag aatcaatcac agcattgaat
     2221 gacggcttga tggcatttac tggctcaatc ttatttgcgt cacatgacca tcaatttatc
     2281 caaacattgg caaaccgcat cattgccgtt tcggataaag gtgtgatcga tcgagctgat
     2341 acgacatatg atgaattttt ggaaaatgca gatattcaaa aacaattgaa cgagttatgg
     2401 gcataatggc ctatttagct taaaaaataa aaaaacagcg acctctaaga gataggatgc
     2461 tcatctatct tttaagagtc gctgtttttg ttacaatcaa tttctgggct tttttacaga
     2521 atgagttact gcttttatcg actgattccc atcgaaaaaa tcgcgataca gccatagcct
     2581 gtgtgactgg cgatcgttgg tcctaaggga taaagcttca catctttcat ctttatctgc
     2641 tcttccaaaa gccgttttgc cttctccgcg ctctctagat ccccagcgta agccacataa
     2701 acgatttgat caagtggtgc aacgattgtt tttaccgtct cattgacgat tgtctggatc
     2761 gacttgtttc ttcctcgaac tttgtcaaca ttcgtcagcc ttccttcttt atccaccgta
     2821 ataataggct ttacactcaa tagactgcca agagtagcac ttgctttgga aatcctaccg
     2881 cctcgttcta gatatttcag atcgtctact gttacccatg aatggacttt caaataatta
     2941 ctttccagcc actctagggt ttcttcgatg cttttacctt cttttttcaa tcttacagct
     3001 tccaatacta aaagcccttc cccaatactt gcagcttttg tatcaaaaac atagatctca
     3061 gggtcttcat attcttcctt cagcaattca accgcttgaa gcgcactact gtaagagccg
     3121 cttaatccac tggaaaaagc cacatataaa acaggaatct gtttttccgc ataagaacgg
     3181 aaaaattcta aatatcggcc aacattgatt tgtgatgtcg taggcatcgc accatttttc
     3241 agctgattca tgtagcggtc ataattgaat gtctttccga ggtcatctac taactcttga
     3301 tcattcaatt ggagattcat tgataaaaaa tcaacttttt cttttgctaa tacatggtat
     3361 ggcagatcac aacaagaatc tgttacaatt tgatacacat ttttcaaccc cttctcgtta
     3421 cattttaccc ttatcgtcta ttatagcaaa tctcttgcgc tagacacact ttaaccggtt
     3481 tattttcgca agttctaatc aaaa
//
LOCUS       DACENJ010000215         3463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.222, whole genome shotgun sequence.
ACCESSION   DACENJ010000215 DACENJ010000000
VERSION     DACENJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.222"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2707
                     /locus_tag="IW748_002372"
     CDS             <1..2707
                     /locus_tag="IW748_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1105270.1"
                     /translation="SSKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYK
                     ETPVSEEKNTLQIDTKDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSG
                     KTYFAHAMYHFAKAHHVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEK
                     MGIIDQADGGMLFLDEIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATT
                     EDPGSSLLETFVRRIPINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVA
                     KALIGSVTYGNIGQLKSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNR
                     SAMAELSKILEPKITVAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAIN
                     HFISTDINIHLKSFYKDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQN
                     FIYAMSLHISSFLKKINLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIP
                     ESEDYYLTVLLVSLRANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPL
                     DMDPKTALDRIERNVQEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSL
                     VLETVRKVSVLGTDLDMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEG
                     TAQRIKELIERAVNKRQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAP
                     FIPLERFIDQNIEVILDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKL
                     IDPMWQFSTQISQTTGIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTND
                     PLYSQLAASVVLLEDQIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            2898..3389
                     /locus_tag="IW748_002373"
     CDS             2898..3389
                     /locus_tag="IW748_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1105271.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
BASE COUNT         1159 a          585 c          757 g          962 t
ORIGIN      
        1 tagcagtaaa gatctgaatc agctagtgcg cgaaggaaaa ctgataaaga cagacggacg
       61 accagtacgt tacatatatc aaacactcgt tacgcacgta aagccagtta caaaacatgt
      121 agtcagttac aaagaaacgc cggtctctga agaaaaaaat acactccaaa tagatactaa
      181 ggatatcttt gcaaaaatca ttggagctaa cggcagcatg aaaaattcag tggaacaggc
      241 aaaagctgcg attttgtatc cgccaagagg attaaattgt ttgattacag gacctaccgg
      301 atcaggaaag acttattttg cacacgcgat gtatcatttt gcaaaagcgc atcatgtaat
      361 agatgaggaa acggaactga ttgttttcaa ttgtgctgat tatgccaata accctgagct
      421 tttgatgagt catttatttg ggtacgcaaa aggtgctttt acaggcgcag atgaagaaaa
      481 aatggggatt atcgatcaag cggatggcgg tatgctgttt ttggatgaga ttcaccggtt
      541 gccgcctgaa ggtcaagaaa tgatctttta ctttatggat catggaactt acagtcgtct
      601 tggggaaaca acgaaatccc atgaagcaaa tgtacggatc gtaggtgcga ccacagaaga
      661 cccaggttct tctctattag aaacttttgt tcggcgtatc ccaatcaata tcaaacttcc
      721 ggcttttgaa aaacgtcctg caaatgagaa agtcgattta gtaaaaatca tgattgccca
      781 cgaagcaaac cgcacgcagc gaaagatttc tttgacagaa gatgtagcaa aagcattgat
      841 cggcagtgtg acctatggaa atataggaca attgaagtca aatatccagt tagtatgtgc
      901 acgaggattt ctaaatcata tgcaatcacc cgaaatctcg atcacaatcg atgatttgac
      961 agaagggatt cgtagcggat tgattcaatt agcaagcaac cgcagtgcaa tggcagagct
     1021 ttctaaaata ttggagccaa agataactgt agcaccaaat gatacgatca tgaaaatcca
     1081 atcggactcc tatgagttac cttataatct atatgatatc atcggagata aagcagcttt
     1141 actgaagtcg gatggtttgg atcaagaagc aatcaatcat ttcatttcta cagatatcaa
     1201 catccatctg aagtctttct ataaagacca tggtttttct tttaatgcag acagcaaatt
     1261 agcagaattt gtagatccga aaatcataga agtgaccaat cagatttatg taatggtcaa
     1321 aagatcttta ccatatgagt ttcagcaaaa ctttatttat gctatgagct tgcatatcag
     1381 ttcttttttg aaaaaaatca acttaggaga agaacggcat acgaatgata acatccgtga
     1441 gatggctttt gattttccag atgaatatgc tgttgcgaaa gaagtgcgta gttatatcga
     1501 aacgtatttt caagtggaga ttccagaaag tgaagactat tacttgaccg ttctactcgt
     1561 ctcgttacgg gcaaatcaag caagcggcag aatcggtgta gtcgttgcag ctcacggaaa
     1621 cagcacagca agcagtatgg tccaagttgt ccaacagttg ttagatgcag agaacgtacg
     1681 agcagtagac atgcctttgg atatggatcc gaaaacagca ttggaccgaa ttgaacgcaa
     1741 tgtacaagaa gtagatgaag gaagcggggt catactctta gtagatatgg ggtcccttgc
     1801 gtcgtttaat tcgcaaattc aaagagacac tggaattcca gtacgtactg ttgatatggt
     1861 aacaacttct ctagttttgg aaactgtacg aaaggtctca gtattaggga cagatctaga
     1921 tatgttgcat gaatcattga aaaattttcg aggttacgca gaaatatctt ctgtagaatc
     1981 tgttcaggaa gcaaaaccta ttcataagaa aaaagctgtt ttggctatct gcgcatctgg
     2041 cgaaggtaca gcacaaagaa tcaaagaatt gatcgaacga gcggtaaaca agcgtcaaga
     2101 aacagaatta gaagttctag ctttgtcgat tgtggaagtg aaagaagaac tgccagttat
     2161 ccaaaaagaa tatcagatca tagctactac tgggattact gatttgaata tccaagcccc
     2221 atttattcct ttagaacgtt ttattgatca aaatatcgaa gtcattttgg atcagttgct
     2281 gatggaatca gaattggagg aaactgaatt catctcccta gatgaagagt cagctaaaaa
     2341 tacgtgtgtt gagtttatct ctgataactt catctttatt aatggtagta agttaatcga
     2401 tccaatgtgg caattttcta cacaaatctc tcagacgacg ggaatcggcg atgaagaata
     2461 tggattcaaa atcaacctag tgatgcacac agcaggaatg atcgaacgta ttattcggaa
     2521 tgaaccattg actgtggaag aaaacgaatt gacaaataca acgaatgatc ctctttatag
     2581 ccaattggca gcctcagttg ttctgttgga agatcagata aaagtaaaag tgccgataga
     2641 agaaatgtat tatctgctac gtctagtcca taatcagtta gataaaaaag aatacactgt
     2701 gccatgaata cacaaaaacg atacactttt atagtgtatc gtttttgtgt attggttaag
     2761 aatcctttaa attcaattag tgtatcgtaa aaaagttggc acgctttttg cttatagtag
     2821 tgtgtgctag gaggtggttg ttcacacaga aatgataggg gtttctgtga agtagtgagg
     2881 aagaaggagt tttgcagatg gatattcgat tggcacgtat agatgatcgt ttaatccacg
     2941 ggcaagtcgc aacagtttgg tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg
     3001 atgtagtcgc tcaagataaa ttgagaaagt ttctactaaa agaagctgca ccgccgggga
     3061 tcacagcaaa tgtaataacc gtgcagaaaa tgattcaaat ctatcatagt ggattattat
     3121 cacgagcaaa agtgatgctt ttattcacca atccccaaga tgttgaagcg gttgtccgct
     3181 gcggtgtaca gttgaagtcg ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa
     3241 tgattaccaa ttttgtttca gtaaatcaac aagatattga ttcctttcgt tacttgaaca
     3301 gtcaagaagt ggagttagag attcgaaaag tacctgctga caggaaaatc cttttgatgg
     3361 aactactagc gaaaatcaaa aaggtgtagc aagaatggtt gttggaaaag gtcaaataat
     3421 tctgttgtaa tggttagcaa ttattaatat ttttgaattt gtt
//
LOCUS       DACENJ010000216         3443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.223, whole genome shotgun sequence.
ACCESSION   DACENJ010000216 DACENJ010000000
VERSION     DACENJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.223"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            195..1043
                     /locus_tag="IW748_002374"
     CDS             195..1043
                     /locus_tag="IW748_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1105272.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1188..2129
                     /locus_tag="IW748_002375"
     CDS             1188..2129
                     /locus_tag="IW748_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1105273.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2119..2985
                     /locus_tag="IW748_002376"
     CDS             2119..2985
                     /locus_tag="IW748_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105274.1"
                     /translation="MTIKREKWLRGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSILWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYWYAPFLSLMAYFMMLTVFRKKDLLYEMIAV
                     VTVLFVYLAARQFFSIRLLSRNTRFQSLTTVLLICFLTFSAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSG"
     gene            3082..3417
                     /locus_tag="IW748_002377"
     CDS             3082..3417
                     /locus_tag="IW748_002377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1105275.1"
                     /translation="MTLKKQAFLPYPYGNTTLTDFSLASGQTLYYLPESQRFIPVPNE
                     STRSATLQTERLVFTREELKSSQLKADDLENQQTNLQLPKSLPKRIEDLAVELTKNEA
                     TLIRKVEAV"
BASE COUNT         1063 a          658 c          647 g         1075 t
ORIGIN      
        1 tactttaaaa aaacataata agatcacttt gcttgtagga tgtattccct tccattacaa
       61 tgaaataaat agcgatttta tgagaaggtg atgtgaaaat ggaagaaaaa atagaagaat
      121 ccaaacaaaa aatcgatcag ttgatataag aattagaaaa agcggtcatc ggcaaacgtt
      181 caactatcca attgatgatc gtggccctat tagcaggtgg tcatgtctta ttcgaagata
      241 ttcccggtgt aggtaaaacg ttactggtca aagcactagc aaaagctgta cagggaacct
      301 tcagcagagt ccaatgtact cctgatctgc tgcctagtga tattcttggt ttttccgtat
      361 acaattcaca atcaaaagag tttgaatttc gtccgggacc gatctttacc acgatcttgt
      421 tagcagatga gatcaatcgt acgactcctc gtactcagtc agcactttta gaagcaatgg
      481 ccgagaatca tgcaacgatc gacaacaata cataccctct agacaatcat ttttttgtct
      541 tagctacgca aaatccaatc gagtacgaag gaacatatcc tttgccggaa gcacagctcg
      601 atcgtttttt attccgacta caaatcggtt atccttcttt tgatgacgaa cttttgctac
      661 ttttggataa attcgaaaaa gtgttggata acttaaatga agtattgaac agttatgaaa
      721 tagaagaaat aaaaacaaat gtggcgaatg tctttgtcag tcccgaagtg gcatcttatg
      781 ccttgcagtt agtgtcagca acaagaagtc atgctgccat ccagcttggc atcagtccac
      841 gaggaagcct ttcctttatt caggcagcaa aagcttatgc tttaatccat gggagaaatt
      901 acgtcacgcc caaagatttg caggatttag ttccttacgt gttttcccat cgcctgattt
      961 tttacgatcg ttcattgaag gaagacgaaa agaaacaaat cattcatact cttgttgcac
     1021 aaattcctat tcctgtgagg tagattgagg tagacaatgg aaaaaatcaa agattatttc
     1081 attaattttg tgttatttct ggtatatttc atcattgtgc tttttgctct tgtattcaac
     1141 aattctttag gctggttctt gttggccttt tttactcttt atgtatcatg gagcttgatt
     1201 tcattaagca gctttctaaa gaaactttcg gttacttgta cattgcagcc actcgtttat
     1261 cgcaaaaaaa gtatgcaggt tattttttcc atagcaaaaa agagaacttt ttctttacct
     1321 ctccctcgtt tacaaatcgg cttcccagca atctttgcag aaaaaaacaa gagattttga
     1381 ttcttacaaa taaaccgcaa gaagtccaat tgctttggca gccaaaagaa agaggatgcc
     1441 atgaaacatt aactgtcatt tttacagcct atgatagtct aggacttttc agaaaaagtg
     1501 caaaaagaga gatctctttt cctgtgactg ttctgcctgc tttttgtaag attcaagcag
     1561 aaaaattaca gagagttctg gaaaaaaaac agcagctgaa cgcgtaccaa agaggtcttg
     1621 attttcggga acatcgcgct tatcgtccag gtgattcttt caaccgaatc gattggaaat
     1681 tgtctgctca tactcaagag tggttatacc gtgaatacga ttatcaagaa gaagaatgtt
     1741 ctcctctttt ttgcttttgg ggttcgccca gtccaaaatt tgaacctctg ctagacttgt
     1801 atttttcttt atggcatttg agaaaagaaa agcagccgga acttctgata ttaggcaacc
     1861 gcgcctttca tggcaaagac cccggtcata cctattttgc ttctttggaa gctggcgatg
     1921 agaaaacttt tttcgcacac ttgaaaaaat atgcaaaaaa acaaatcgtc ctctttgttc
     1981 ctgagaattc tcctgaagtc tcagaagcta ttgaaacatt gtcaaaagat cgaacggttt
     2041 atcttgttta tttcgtagaa aaacaactca tggtccaaga aaaagacaag atatgttcat
     2101 tgccaggagg tgaatggatt gacgattaaa cgggaaaaat ggctgcgtgg cgtttactcc
     2161 tttgtgatac taagtgtagg tgctccgata tttgtagtag ctaatcaatt gcaaaacagc
     2221 taccttctta tcctaactat tggtgtccta tgccttattg tctgctgtat tcctaaaaaa
     2281 tgggtacgtt tgctgctttc tttgccagta atgataggtt gtttgttttt atattttcct
     2341 tcgaagacat tttccatatt atggatctta cagttttttt cgtcagttac agaggaattc
     2401 ttccatctat cccaaggtaa actttattat ttgccagaaa agacagcttt ttttctcata
     2461 atgttgctga tctatttatt tattacattg accgtggatt acgactattg gtacgcaccc
     2521 tttctttctt tgatggctta cttcatgatg cttaccgttt ttcggaaaaa agatttactg
     2581 tacgaaatga ttgcggtagt aactgttttg ttcgtgtatc tggcagcaag gcaatttttc
     2641 tccatccggt tgttgtcgag aaacactcga ttccagtcat tgactacagt tctgcttatt
     2701 tgtttcttaa cttttagcgc tatcttgcct ttatatcttc cgaaagtaca ccaatctctc
     2761 gaggaaacga gtgaaccgat ccgagaatat cttaatgatg aagggcttta tgacacactc
     2821 catgagtatc aattaaccgg aagtgcaaga acaggcttca gtgagaatga tgaacagctt
     2881 ggtggacctt tatatgataa tggcgaagtt gtttttacag cgaaccaaaa aggaaatggc
     2941 tattggaaga tcgaaaataa aaatcattac accggttctg gatgaataga agatcaggac
     3001 gcacccatcc aaccaatcca agaattccct gttgacatca cgagctacca aaccgttata
     3061 gaagacaaag aaaacattac attgacccta aaaaaacaag ctttcttacc ttatccttat
     3121 ggaaatacta ccttgactga tttttcatta gctagtggac aaacgcttta ctatttgcct
     3181 gaaagccagc gattcattcc tgtcccgaat gaatcgacac gttctgcaac tttacaaaca
     3241 gaaaggttag tttttacgag agaagaatta aaaagcagtc agttaaaagc tgatgattta
     3301 gaaaatcagc agacaaatct tcaattgcca aaaagtttgc ctaaacgtat tgaggacttg
     3361 gcggtcgaat tgaccaaaaa tgaagcaact ttaattcgta aagtcgaagc tgtctaacag
     3421 tatttaaaaa caagcggaaa ttt
//
LOCUS       DACENJ010000217         3389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.224, whole genome shotgun sequence.
ACCESSION   DACENJ010000217 DACENJ010000000
VERSION     DACENJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.224"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..717
                     /locus_tag="IW748_002378"
     CDS             <1..717
                     /locus_tag="IW748_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1105276.1"
                     /translation="EATKKAREFGAMTICLSNEEGSPLWEAAEYPVHYDWGKDVDASD
                     LNKGILYGLLFNILLVLDGSKKWETCLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRD
                     KIIYTIGSGINYGEIYSTAACWFMEMQWINSNAIHSGEFFHGPFEITDYDVPFILVKS
                     IGDTRFLDQRVEDFTKKFSNKLMVLDQADFDLESIDSVAKEYIAAVLSGVVIRQMVEA
                     IAFERGHSLDVRRYMWQMKY"
     gene            699..1160
                     /locus_tag="IW748_002379"
     CDS             699..1160
                     /locus_tag="IW748_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1105277.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1210..1674
                     /locus_tag="IW748_002380"
     CDS             1210..1674
                     /locus_tag="IW748_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1105278.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1688..2437
                     /locus_tag="IW748_002381"
     CDS             1688..2437
                     /locus_tag="IW748_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1105279.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2437..3267
                     /locus_tag="IW748_002382"
     CDS             2437..3267
                     /locus_tag="IW748_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1105280.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT         1125 a          444 c          660 g         1160 t
ORIGIN      
        1 gaagcaacaa aaaaagcaag agaatttgga gcaatgacta tttgtttatc taatgaagaa
       61 ggatctcctc tatgggaagc agctgaatat cctgttcact atgattgggg taaagatgtt
      121 gatgctagtg atttaaataa aggaattctt tatggtttat tatttaatat tttacttgtt
      181 ttagatggct ctaaaaaatg ggaaacttgc ttgaatgaat ttgataagtt aaatgatctt
      241 gttattgctg aaaagagcaa atttgaaata actgctaaag aatggggtaa aaaccaaaaa
      301 cgagataaaa ttatttacac aattggtagc ggtattaatt acggagaaat ttactccaca
      361 gcagcttgct ggttcatgga aatgcaatgg attaattcta atgctattca ttctggggaa
      421 tttttccatg gtccttttga aatcactgat tatgatgtac cattcatctt agtgaaaagt
      481 attggagata caagattttt agatcaacgg gtagaagact tcactaaaaa attcagtaat
      541 aaattaatgg ttttagacca agctgatttt gacttagaat ctatagatag tgtagcgaaa
      601 gaatatattg cagctgtact tagtggcgtt gttattcgcc aaatggtaga agcgattgct
      661 tttgaaagag gccattcctt agatgttaga cgctatatgt ggcaaatgaa atattaatta
      721 ataggaggtt gcaaaagatg gcaaatcaat tatttcacat tgtgattaca acacattcta
      781 ccctttgtga gggttattta aaggcaacaa aattaatttt aatgaatgac gccacaggta
      841 ttagtgctgt tccatttaaa gaaaatacta gtccagatga ttttgaggag agtatgaaag
      901 aaataattag tcagcatcat ttgcaacctc taattatttt aacggattta attggaggaa
      961 cacctaataa tgtttctatt aaacagttga acaacaatag aattcaaatt attgctggga
     1021 ttaatttacc tcttttactg gagttattaa tggcccagtc agctggtaaa tctatagaag
     1081 aaatagattt taaggaatta ctgaataact ctcaaaaaag tatggttcat attaattcgc
     1141 ttttaaaaga tgtcagttaa ggtggcaatt tctattctat aaattttaaa aattgaagga
     1201 ggaaatagaa tgattaaatt agtgcgtgta gaccacagat tacttcatgg acaagtaata
     1261 ttttcttgga ctaaacagta tgatattaac catattattg tggctgataa caaagttcca
     1321 ggagatccaa tgtctgtgat ggctttgaca attgctaaac ctgctgattg caaattagac
     1381 attgttaaaa ttagtgatgt taaagggatt atggaaacat ccccaaatga taattacatg
     1441 attttaatta aaggacctaa ggaagctttt gaattagttc aaaatttacc agaaattaaa
     1501 gaaattaatt ttggcggtgt gtctaagaaa agcgagtcca aacaatatgg aaaggcagtc
     1561 tttttaactc ctgaagaatt agaagccaca aaaaaaattc ttgatcaaga agtgaaaata
     1621 tttgtgcaac aagtcccatc ttcaagtatt gaaaatgtag attttagtaa gtaagggggg
     1681 aaaataaatg ttattaaaag ccattttatt aggtttagtt ggtgtggtag ctgttattga
     1741 ctccaggtta attggtcgac aaaatattgg tagaccacta attttgagta cacttgccgg
     1801 acttgtgcta ggtgacttgc atacagggat tgttcttggg gcatctttag aattaatttc
     1861 tatgggattc gtctcaattg gggcggctgg acctcctaat atgcaacttg gtagtattat
     1921 tgccactgct tttgcaattt taactaatag tagtacagaa acagccttaa caatagctat
     1981 cccagtggct gtagctggtg aatttatgag tattatcatg cgtatgataa ttgctcaatt
     2041 tggacatgct gcagataaag ccattgaaaa taataaattt agaaaagctc aaattattca
     2101 tatttattgg tcatttattt ttaatgcttt tgtttacttt attccaatct ttttaacagt
     2161 atattttggt gctgacgttg tggcagattt agtagataaa attccagaaa tgattacaga
     2221 cgccttgact gtctctggaa atatgctttc cgcattaggg tttgcaatgc tattaagcac
     2281 aatgttgagt aaaaaattat atccttactt tatttttgga ttctttattg ttgcctattc
     2341 aggtctttca ctaattggcg tgacaatttt tgcagctctg attgcctttg taatggatca
     2401 agttaaatat ggaaaaagag aagaggcgca tggttaatgg aaaatagtac agaaaaaaaa
     2461 cgtttaatca caaaaaaaga tcttcataag atgtgtcttc gttcatggac aatcatgtgt
     2521 tcgtggaatt atgaaagaca aatgcatatg ggatttatgt atgggatggc gccaatttta
     2581 aataaagttt ataaaaatga tgaagagaaa aaaagagaag cttatactcg tcatatggca
     2641 ttttttaact gtacgcctca aatgactcca tttattatgg gactttgtgc ttctatggaa
     2701 gaacaaaatg ctcaagcaga agaaggaaac tttaatactg aatctatctc catgattaaa
     2761 accagtttaa tgggtccttt tgctggaatt ggtgattcat ttttccaagg tactttaaga
     2821 attattacat ttgggattgg attgtctttt gctaagcaag gtagtatttt aggaccgatt
     2881 ttagctgttg gcctttttgc tataccttct attttagctg ttttttacgg cggtaaatta
     2941 ggatatgagt ctggcaataa ataccttact aaattgtatc aagaaggtct tatggatcga
     3001 gtaatgcaat ttgcttctat cattggtttg gctgtagtag gtggaatggt ggctagcatg
     3061 gtgccaattg tcaccccttt aaaattcact acagggggaa cagaacttgt tttacaagac
     3121 ttacttgatt ccattattcc tggattatta ccacttgtat gtactttggg aatatataaa
     3181 ttgattaaga aaaatgttag tacaaatatg ctcttattag gttgtatttt ggcagggttt
     3241 gttttaagta taataggtat cttataatga ttaaggctct aatttttgat atggacggag
     3301 ttttaattga tagcgagatt atgtatcaaa agtgggtatt taatttttta aaacaagaaa
     3361 atttaagcta tcctttgtat tcttatcaa
//
LOCUS       DACENJ010000218         3381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.225, whole genome shotgun sequence.
ACCESSION   DACENJ010000218 DACENJ010000000
VERSION     DACENJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.225"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(246..749)
                     /locus_tag="IW748_002383"
     CDS             complement(246..749)
                     /locus_tag="IW748_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1105281.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1108..2133
                     /locus_tag="IW748_002384"
     CDS             1108..2133
                     /locus_tag="IW748_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1105282.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2480..3358
                     /locus_tag="IW748_002385"
     CDS             2480..3358
                     /locus_tag="IW748_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105283.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         1082 a          534 c          636 g         1129 t
ORIGIN      
        1 tcctttctag attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa
       61 aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg
      121 tcctgctgca ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg
      181 aataaaaaga ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa
      241 cgattttatt gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag
      301 aataaaatgt atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg
      361 tttagaagtt gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca
      421 gtgatgtccg ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca
      481 aggaaaataa tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc
      541 accccaaaag gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt
      601 tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat
      661 tgtggaagat taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc
      721 cctaataaaa attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat
      781 cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta
      841 cgaaaattag aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg
      901 agttgcttgg aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt
      961 gttctaattt ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata
     1021 gttgttatac taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt
     1081 ttccaattac taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata
     1141 caagataatc cttttcgtat catgaatctg atgctactta tctttattgt attatggtta
     1201 ggttacataa atcatgattt tttgatttca agtttttcat cattagggat ctttactttt
     1261 ttctattatc aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc
     1321 ggactgttgg ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca
     1381 tttgccgtag ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct
     1441 aaaccaggtg gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca
     1501 atcgctcaat taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt
     1561 gcggcagtga ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact
     1621 ttaaaagaaa gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct
     1681 gccttatttc tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg
     1741 accttgtcat gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa
     1801 gtccaatatc tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg
     1861 ccgtttacgc agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt
     1921 ctagttgcta gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta
     1981 tctacactta ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc
     2041 gtcataggaa gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat
     2101 tatttaacgg ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg
     2161 ttatgacaag atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc
     2221 attaagtcaa attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt
     2281 ctatcaaaaa attttatatt tattctatca aaaaattttg tttatgatgg taactgaata
     2341 aactttcctg agatggtcaa tgctttcctt gaaagtctta cagatattct ttcttcatag
     2401 aaagtttaag ttttatagtt agtgtacaat aaaagtatgt ttataccata tggtgtccgg
     2461 ataatcagtc atttaaacaa tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa
     2521 aataagcaat gaaatccaaa ttctggcaga aactttaact cttcccaaag tcgttataga
     2581 gcaaatgatt gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat atagtcatga
     2641 actaatggat cctaataaag caaaggcgac tgcaaagaaa atagacaaag tctatgaaga
     2701 ggaacctttt gcagcattag ctatttatct atatgcagct tctcaaagtt ggaaaaaaat
     2761 ttatcaacca ttgaagattc caagaactat ctatttggca acaatgaatg cttttactag
     2821 atttattcat gaacatttcc aagtaacagg aagctatcgc tttgaccgtg gtttttggat
     2881 ttggcattac actagtggct tgatctttcg gattggtgaa ttggagtacg aaatgacata
     2941 ttttccgaaa gggtacacgg atctaagatt agaaggaaag cagtgccttt ctcttcatat
     3001 tccttccgat gcagatttat ctcagaataa gataagagat agttacgaaa gtgccattcg
     3061 tttttttgaa ctttattttc ctgattacca ttaccaagtg atgtacacag atacttggtt
     3121 actatcaccc aatcttacta aatggttgaa aaaagagtca aaaatctgtc tttttgcagc
     3181 cgattatcga ttattatcag ttgatgaaca ggacgatagt ggagtccctt ggatctttgg
     3241 acgagtagat gcccaaatac atgattatcc agaaagtacg agtttacagc gacaagcaaa
     3301 agagcaattg cttgcaggcg agcacattgg ctctggctta ggtattctta cttcataagt
     3361 ttagtacttc gaaatagttt a
//
LOCUS       DACENJ010000219         3333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.226, whole genome shotgun sequence.
ACCESSION   DACENJ010000219 DACENJ010000000
VERSION     DACENJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.226"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(350..1390)
                     /locus_tag="IW748_002386"
     CDS             complement(350..1390)
                     /locus_tag="IW748_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1105284.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1739..2065)
                     /locus_tag="IW748_002387"
     CDS             complement(1739..2065)
                     /locus_tag="IW748_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105285.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2052..2840)
                     /locus_tag="IW748_002388"
     CDS             complement(2052..2840)
                     /locus_tag="IW748_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1105286.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1019 a          537 c          450 g         1327 t
ORIGIN      
        1 actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa aagaagtaat
       61 atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat tattataact
      121 caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa taactaaaat
      181 atctaacgat tatgacttta aaacctacgt ttgtgattgt tttcataaac ttttaatcta
      241 aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa aatcataaaa
      301 aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac tactctagcc
      361 aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg tttgcaagct
      421 ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt agattctgca
      481 aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc atatataatc
      541 ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact gtttgctcta
      601 attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga gcgtcttcgt
      661 aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat gtatttatac
      721 tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt aactgatcag
      781 tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat agattgtctg
      841 cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta tttgcgattt
      901 tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg attttcgcaa
      961 ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct tgattcgtta
     1021 cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg tcacgtttat
     1081 ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt ttgattttag
     1141 atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg aatatgaaaa
     1201 atatattatc atcagaatct acccagttat ttttgactga ataatcaaat cgatctttaa
     1261 gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg ttagtaaaaa
     1321 ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg taataattaa
     1381 aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac tatgttacaa
     1441 tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa gtcgccaaac
     1501 tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa ataaattaaa
     1561 gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata ttacaataat
     1621 attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca gctttgatac
     1681 tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg aatgtaaatt
     1741 aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct tcaggtatgg
     1801 actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca gcaatagtcg
     1861 tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct gctgttctta
     1921 cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat tcgatttctt
     1981 ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta cttactgcag
     2041 cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata ttcttctgta
     2101 aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag atctgatcca
     2161 gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa aatttttgca
     2221 ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat gttataacgc
     2281 ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt atattctttt
     2341 accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata cgcagcttca
     2401 actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat tcctaaactt
     2461 ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag tactctttta
     2521 agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag ggctatgtcg
     2581 gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc agcctcccct
     2641 tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc tacattatct
     2701 gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta aacaaatttc
     2761 aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt ttgattggct
     2821 actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata atataattat
     2881 atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat tgtgtgatta
     2941 ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt tattcgaaat
     3001 taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa tatgttttgc
     3061 gagcagcaac tagaataatt taaacattac taaactgtct atattgttac taataattcc
     3121 cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat attaagatat
     3181 cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt tatcatgttt
     3241 aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa catcagacat
     3301 gtttaaacta gaggccaggc ttaaatcata aac
//
LOCUS       DACENJ010000220         3257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.227, whole genome shotgun sequence.
ACCESSION   DACENJ010000220 DACENJ010000000
VERSION     DACENJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.227"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            55..762
                     /locus_tag="IW748_002389"
     CDS             55..762
                     /locus_tag="IW748_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1105287.1"
                     /translation="MFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            759..1526
                     /locus_tag="IW748_002390"
     CDS             759..1526
                     /locus_tag="IW748_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1105288.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            1626..3194
                     /gene="liaX"
                     /locus_tag="IW748_002391"
     CDS             1626..3194
                     /gene="liaX"
                     /locus_tag="IW748_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1105289.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSVIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
BASE COUNT         1083 a          530 c          685 g          959 t
ORIGIN      
        1 gaatggcagc tagtagcaaa aaaaaatgat ggctggcttt tcttcttcac aagtatcttc
       61 attggcgcca tgttgcttat tggcggaaaa catggtctat ttgctttagg aagtctcatc
      121 atcaatatcg ttttactttt acttttgttg ctcatcttta ctaaaataga agggatttct
      181 ctattaaata tatctatcct gttcgtcgtt atagggatga ttattagcat attggcttta
      241 gatggatgga atagaagcag tataatcaaa atttcagctg cagttacgag tgttttttta
      301 gcatttttta tttgtctttt gacaatgaat ctttggaaag accaaggact gcgctatgag
      361 gaactgaatt atctgacacg accgtatcgt tctgtttttc tttctagcgt actgattgga
      421 acagctggag gaacactcga taccgttatc acagtcattg cgacattaga agaattgact
      481 aagcgacaac cagaaataac agcgaaacag ttatggcttt cgggaaggca agtgggtcga
      541 gatgtgattg gctcgatgac caatatcctt ttgttcgtct atatcagtgg gtcagttcca
      601 tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag aaacgataga acagcattta
      661 tcattggaat ttttacgcgt cattgctggc agcttaggaa tagtattatc gatccctatc
      721 tcggtcctct tttttctgac tgtaaggagg ctgaaacgat gaatgtactt attatacttg
      781 ttgggatttt agcaatcagc gtgctactta ttggcggtac gcaaggtatg tatattcttt
      841 taggcttatt catcaattta gggatctttt tcttgttgtt attcggttat catcagaagt
      901 ggccgatact ggcattgtcg gtcataggat ttttattgat tgctgtggtt atcttatttt
      961 ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt gagtatactg atctttttat
     1021 tttgtttcct actattgctt cctatcactg attttttagc aatccaaggt tttacaacga
     1081 tagaactaga agaattatct ggcttagata agactttagc gattgatttt agattactta
     1141 cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt agatgcgagt gtagcaattt
     1201 ctagtgggac attcgaagta tatcaggcaa atccgcaatt atctttttac caattgcgcc
     1261 attctagttt cgctattgct aaaaaaatat tggcgtcaac agtaatgaca cttctttttg
     1321 cattcatggg aagttcttta gcgttgatcc tctggtttat tgatttagac attccttttc
     1381 agcaagtgat caatgaaaaa tcatttgttc tagaatatac gatggctatt cttaccacat
     1441 tgtccgcact acttgttctt cctttaacct gcgtgactac agcatatctc tttacaaaaa
     1501 agaaagaaca actcaagatg gagtaaaagt tacaacttaa gtcctatgac atttttactg
     1561 aatcagtgca taatatggat gtaaggaaaa gcttgcaaac aattttgctt aaggaggcaa
     1621 tttccatgaa agaaagagaa cgtattttag atttagtgaa aaaaggaatc ctttcgactg
     1681 aagaaggctt agatttatta gaaagcatgg caactgaaaa agacgaaaag caaatcaaaa
     1741 aagaagcaga caaggtaact gctagtcata aagaaaaaga tcaggcaagt caattgatcg
     1801 ataaacttga aaacggcgaa gaagaaattt ctgagccagt tgatccaaag gaaagagaaa
     1861 gacaagatca agaaaactta gaaaagatct tagatgaact agcaactgaa gcgaataaaa
     1921 cttcggcgcg tctagatgaa gtaaatgctc aattcgctga caataaggca gcacgcaatg
     1981 agaaacagga agcattgatg cagctgaata caaaagaaga attaggcgag ttgacagaag
     2041 aagaattagc acagcgtcag acgttagaag cagaaatcaa agagttagaa gctagtggcg
     2101 atacactgtt agaagaacaa ttgaaattag aagcagaatt aaaagatatc cgtaagaatc
     2161 aatggtctga gaaaaaagaa acgtttactg ataagtttga attgccagat gattggaaag
     2221 atcaagcaac agatacactg aatcaagtag gcgaaaaaat gtcagaagct ggatcacagt
     2281 taggaaaatt cttgaagaaa acattccaaa ctgtttcaga aacagtgaat gacaacatgg
     2341 aatggaaaga tgtcagcttg cgtgtccctg ggattgcgac aacgaaattt gagcatgaat
     2401 tttactatga agctcctgca gcaagtatcc ttgatatcaa ggcagcaaac ggaaacgtga
     2461 cgttaaaaac gtgggacagc aacgatgtga aagttgaagc taagatcaaa ctttacggaa
     2521 aaatgggtgc tgaaccattt gaagcattct ctgaacggag ccagattgag gttaatgaag
     2581 atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc agaccttgtt ttctacttac
     2641 ctaaacgagt atacgaccac gcagctatta aacttctaaa tggcaacatc atgattgaaa
     2701 cattggaagc taaagatatt tatacaaaat ctacgaatgg taacatcatc gtggaccaat
     2761 taactgcaac gatgctggaa gtagaaggtg taaacggcaa tatcgatatt cgaaacggga
     2821 acatcttaga ttctgttatc gaaacagtaa atggtacagt gacatttggt gcgacagcag
     2881 aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct tacgattaaa gaagataatt
     2941 tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa agtcgcttta ccagatggca
     3001 tcggtttgga aggacatgct aaaacaagtc ttggaagtat caacagccgt ctgtccaatt
     3061 acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat gttgcaattc cgtcgtgttt
     3121 cagatggtga aatcgcgcaa gtgcaacttt caacgacaac aggaagtatc tatttaaaag
     3181 atacagataa ataaagaggt tgtgaaagag atgttcagcc ttgagcaata agaagcgatt
     3241 ttgtaaaata gcttccc
//
LOCUS       DACENJ010000221         3219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.228, whole genome shotgun sequence.
ACCESSION   DACENJ010000221 DACENJ010000000
VERSION     DACENJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.228"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..1438
                     /locus_tag="IW748_002392"
     CDS             50..1438
                     /locus_tag="IW748_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1105290.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3219
                     /locus_tag="IW748_002393"
     CDS             1776..>3219
                     /locus_tag="IW748_002393"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1105291.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1183 a          505 c          583 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaag
//
LOCUS       DACENJ010000222         3197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.229, whole genome shotgun sequence.
ACCESSION   DACENJ010000222 DACENJ010000000
VERSION     DACENJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.229"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(144..2201)
                     /locus_tag="IW748_002394"
     CDS             complement(144..2201)
                     /locus_tag="IW748_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1105292.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(2345..2536)
                     /locus_tag="IW748_002395"
     CDS             complement(2345..2536)
                     /locus_tag="IW748_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105293.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            2724..3179
                     /locus_tag="IW748_002396"
     CDS             2724..3179
                     /locus_tag="IW748_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105294.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
BASE COUNT          947 a          526 c          629 g         1095 t
ORIGIN      
        1 gttattgttc ctaaaacagg ttctacacat ttagtaacga ttttagcggt cagcttacta
       61 ttacttgtgt tagcaacttt tggctacgca gttctgaagt tcaattaaac aaaaaaacaa
      121 ggctgaaaat cagccttgtt tttctatttg aaaatgggat tggcactaat aaatacgttg
      181 tctgaagaaa gtgatccgtc tgcagaaact tcataagcat gggataaacc aacgatactt
      241 tgattgttgt aaagcaagtt tgcgttatga gcagcgctat ctttccacat aaccatagca
      301 tttactgctc ctgtatctga aaggatctcg tcaacgtaaa ggtagaaact attgatattt
      361 tctcctttag caccgtagca gcctaagctt tctaagagat cttggaaagt accgccgcca
      421 ggtcggtaat gactgaagct tacttgtaat tcttttgcac gaatatctgc agcttgttga
      481 acgatatcat tgaattgcat tggggcaacg ccattttgtt gacggtagct atttaaccaa
      541 gcaagttctt tttgacgcac atagttgttt aaatcactta gtgagatatt tttgtatttg
      601 aaagtgatcg ttcgattttg gtcaacatta ttgattacac gttgtccatt tttatcaacg
      661 gtaacagatg aatcgtcatt tctacacaag aagtagtttt gatcggtcat atttttccaa
      721 gaagtagaga aactatctcc ctcgtaaacc gtttgtgttt cagtagataa aacagcgttt
      781 gtgtcaacgt ttatatggtt cacagtgacg ttgtattgat gagtataggc aaaattgatt
      841 ccttgattac ttgtaacgtt gtttaaagtc acagtgttgc tagagctttc tctaggatca
      901 agaacaaaat tagtgaacat aggtgcttca attgtgatgc tttgtccata aacgacggga
      961 taactcttac tgtaattata gagttcttgg atatttggat cagtatccga gactttattt
     1021 ggacgagctt tcacttcaac tagaaagact tcatcatagt aaaaagtaaa tgtggtattt
     1081 gtgttaatcg tttgtgtttg tgtttcattc ccatttactt gatactgtgg tgcttcaaag
     1141 ttagtcaagt cggttctagc ttttgcagtg taacttgtac cagcttttac tgagacatgt
     1201 tcttctttta gcagcgttcc agtttgtcga ttcatatgac ggacagttac cgcatattct
     1261 gaaggtttag gtttgtgttg taacccttta aatgcagaat caagactatt tttagcatta
     1321 tttacttggg tttgagaagc tttagaatcg tctagtactt ttttagcatt ggataaagca
     1381 gtctgaaaat tattccaaga tccttctgta aaatctcctt tagcagttga ttttacttgg
     1441 ttgtacaagg cttgtaattc cgttttgttc acagtaggag ctggtttttt ctttaatccc
     1501 ttgtaagcag aatctaatgt gtttttggca ctatccactt gtttttgagt cgcttttgag
     1561 tcatttaaca cttttttagc attgtttaaa gcggtttgga atgcgttcca ggtattgtcg
     1621 gtatagtcac cttttttagt tcccttaacg ctcgtataaa gagcttctaa agctgattta
     1681 ttcactttgg gtttagaggt gtcagcacca taccaaccaa taccttcgta tttccagcca
     1741 tagtctttgg ttaaccattg tacttctttc caacttgcag tgtagtgatg cgaaccagag
     1801 gtaacacctg gaaggaaaag gcggtagatc ggtttattat ttttttcggc agaacgccat
     1861 gcaggccctt cataagtcca accgtaattt ttggtcaacc atttcacttc atcccaacta
     1921 gctgtgtaat ggtgatcacg aaggccttta ttgtataaac gatagacaag gtggccaccg
     1981 ctagtcggtg cttcccaagc ggggccttca taattacccc acttaatttt cactaggtga
     2041 tcacgttctt ttgtgctaga ggtgtaaaaa tgttcatggt tatttggatt ataaaggcga
     2101 tacatgacgt tagttgaagc agcgtcagca gatccagcaa gagcaactgt ggcagcaaat
     2161 gttccaatag ctataacagt ttttgggttt aataatttca tgttttcaac tcctattaaa
     2221 tgattaaata ttttgtttac attcataata taccacggta taccgaggta gtcaacggta
     2281 taccgtggaa attttgaaat aaaaaagccg ccctatgaag agtgtaagga tggctgttta
     2341 tatgctatga agaaatggat tcggcgtatt tggttagttt ggcagcatta tcaaatcgca
     2401 ttttttcaat cgcacttttt ttctttatta aatccgcaat tgttgattct gcgactgaag
     2461 aatgtttgct aatttcatat gctgtcttgc cagtttcaaa tagtaaccat tcgattttct
     2521 ctttatcagc gtacattgca atgctccctc ttgatttttt ctttattata gctctttttt
     2581 gtaataaatc acaatatcaa gaggaaaagt ttctatagaa aggtgggaaa ttatgagtat
     2641 ccgttccccg tgtatcaaaa tgaggtttgt tgctactttc gttatttgat atactaaaat
     2701 aaaacaacga aagaaggaat agaatgaaaa agttaataat ggtatttact tcgttgattg
     2761 ccatgattgt tattttaggt ggatgtggta gtgaaaaaaa taagcaagat agtcaagata
     2821 atggcgtgtt aaaaccagga acagtgtgga aagaagaagt aggcggactt gttcataatt
     2881 taaaaattat agatgataca acatgggaat attccgaaag tgtttggcat cctgaacaag
     2941 tacaaataac agttgagaag ctaaaagact ataaaggatt agaaagatat aaggtaatcg
     3001 attcaggtgg agtagattta ttcataggta aaacagcctt aatagttccc tataacaaag
     3061 acggtttaga aacgtttaaa tttattccaa caaacgaaaa agaacatcga gataaaagtg
     3121 aaatattaaa agaatacagt actaaatctg gatacaagtt acaaaagact agcgagtgat
     3181 tgctagtctt tttattt
//
LOCUS       DACENJ010000223         3192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.230, whole genome shotgun sequence.
ACCESSION   DACENJ010000223 DACENJ010000000
VERSION     DACENJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.230"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..320)
                     /locus_tag="IW748_002397"
     CDS             complement(<1..320)
                     /locus_tag="IW748_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HAQ1105295.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHAS"
     gene            complement(364..1194)
                     /locus_tag="IW748_002398"
     CDS             complement(364..1194)
                     /locus_tag="IW748_002398"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1105296.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(1209..2147)
                     /locus_tag="IW748_002399"
     CDS             complement(1209..2147)
                     /locus_tag="IW748_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1105297.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            complement(2314..2664)
                     /locus_tag="IW748_002400"
     CDS             complement(2314..2664)
                     /locus_tag="IW748_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1105298.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            complement(2664..2981)
                     /locus_tag="IW748_002401"
     CDS             complement(2664..2981)
                     /locus_tag="IW748_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1105299.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
BASE COUNT          974 a          686 c          555 g          977 t
ORIGIN      
        1 ttacttgcat ggatgatcac tggctttgta ttcaatttag gcactagttc ttttgcaaca
       61 gaacgtattg ctttcgttgg aatgacgaat agcaccgcat ctgcgtcttt tactgcatct
      121 tccatattgg tgaatgcttg gatggaatcg ggtagacaat gatctggtaa ataacgttga
      181 ttcgtgtgtt gggtgttgat ttcttctact tgtgaagcac gatgtcccca aatccgaacg
      241 tcatgtccgt tttcggcgag gacttgtgcc aaagcagtac cccaagagcc tggtcctaaa
      301 accgctactc tataattcat tttaccactc ctgaaatttt tttcattttt aacgctctaa
      361 aaatcagata atttgttgat tttttcctaa tgagcggtca taatccggta aatttccttt
      421 ttttctgcgc caaataaata gaatcaatgt accgacaaat aacacagcag ataataattg
      481 agaaatacgg atcacaccta aaaagtaaag actgtctgta cgtaatcctt caataaagaa
      541 acgcccgaaa ctataccaca ttagataaca taaaaggact tcaccttttt tcaaaaggtg
      601 tggtttcttt cgtaaaaaca aaagcaaaat aaatccaatc aaactccaaa gtgattcata
      661 aagaaatgtt ggttgacgat agatcccttc gatatacata ttatcaatga tgaattgcgg
      721 taaatgcaga ctttctaaga agcttcgcgt cgtgtctggt ccatatgctt cgtggttcat
      781 gaaatttccc caacgaccga ttgcttgtgc taataataca cttggtgcag cgatatctaa
      841 aaaagtccac aaagaaataa aatgatattt agtaaaaaaa tacagcgtga tcgcgcctgc
      901 aatcagtccg ccataaatag ctaagcctcc tgaacggata ttgaatattt ggattggatc
      961 agctatatac tggggaagat caaataaaac ataatataag cgagcgccaa taatggagat
     1021 aggcaatccc cacaacataa aatctgtcac atcatctgct tttaatccta cccgaacagc
     1081 ctctctgctg ctaagccaca tggcgataat gatacctgat acgatgatca atgcatacca
     1141 gtagatagga atccctacaa gctcaaaagc aactcgattg atttgtgcta acatattgtc
     1201 actccttgct agtcgttgcc tgagttttct tcaatcaagc gacttaatct ttcttcaaat
     1261 gttttcgttg cgtcaaagcc cattgtacgt gcacggaaat tcatcgcggc tacttcaatg
     1321 atgattgcaa cgtttcgtcc tgttttgaca gggattttta tttgtggaat gcctacttcc
     1381 gcgatttcaa cggtcgtgtc ttcagagccc agacggtcgt atttcttatc ttttgcccat
     1441 gcttccagat aaacagccaa ctggacttgc atagagccac gtaccgcact cgcaccaaaa
     1501 agattcatta catcaatgat cccgattcct cggatttcaa tcagatgttc caggatttta
     1561 ggcggttctc cgatcaccgt taattcatct tgctgataaa catctactcg atcgtctgcg
     1621 atcaatcgat gaccgcgttt gatcaactcc aacgctgttt cactttttcc tatcccactg
     1681 tcaccttgaa tcagtacacc taatccgtaa acatccacta aaacaccgtg gacacttgtt
     1741 cgaggggcaa ggcgaccatc taaataactg gataattctc ctaataagcg agaagttgag
     1801 atgggagaac gcagtacagc tatcccattt tcatttgctg cctggatcat ttcttctgga
     1861 gctggtaatc ctcttgaaat aataaatgcg ggcatatctt cactacataa tcttctcatg
     1921 atgatcaaac gctcttctgg gatcatccgt tccgcaaaac tgatttcttt actgccaaat
     1981 agctgtaaac gattatggga ataataatta aaataaccag tcaactctag tcctgggcgt
     2041 gagatgtccc cagttacgat gacacggtct aagcttttct cgtcgcctgt cacgatttcc
     2101 aaagataatt ttttcactaa ttggctaact tttacaactt ctgacattca tgctacctca
     2161 tttctattag agtgttctct atcattttat cattgtctag ccaaaagtac taccaattaa
     2221 aaaaaacgtt tttatttaat taccaatgta atagaaaacg cattcctgat tctaaataaa
     2281 aaaactgcgc cgatagttcg gcacagcttt cttttatttt ctactgttat gttcgctgac
     2341 aattacattg acaagcgaca tcaagatagc tactaacaac gctgcaccga atgaagaaaa
     2401 actaaaatgt tctgctccca caaaaaagga ggtcatttta agaatcgctg cattgataac
     2461 aaacgtaaac aatcccaatg tgactaatgt aaagggcagg gacagtatcg tcaaaatcgg
     2521 tttgacaaac atattcaaca cagacaatac gaagctggca agaacggccg tccaaatact
     2581 gctcacatgg accatcgcag gaaaaagaac agccaatgat atgaatgtta acgtattaac
     2641 taaaatacgt tgaaagtacg acattaaaag tcactccatt cgtcatcatc gattttttcc
     2701 gcttctttgc gtttttttgt ttgttcacga gtttgttcac gataattgtt tttttcataa
     2761 tatgcattgt tatgtccata gtttgttcgt gcagaacgcg cagatgggat aatcaaagct
     2821 aataaaatgt ataaaatgat tggacttcca aaagcaacta agctcaggaa tacataaagc
     2881 acacggataa cagtcggatc aatcccgatg tattctccta ttcctgctaa agtccctgtc
     2941 aaaacaacat ttttattgga ttttgtcaac ttttttttca tcatgtctca cctctgttct
     3001 actattttaa atgaagaaaa tcgttgtgac aaccttcttc ttaatcggtg tctttttgca
     3061 aaattctcgg tcacaaatta ccatattcat aaaacgtgac gagctttttt tgaaattatt
     3121 ccttgtgact cggcgataat tggcaagttc ataatctctt ggtaaacggt gttcaagaga
     3181 tagcctatcg gc
//
LOCUS       DACENJ010000224         3152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.231, whole genome shotgun sequence.
ACCESSION   DACENJ010000224 DACENJ010000000
VERSION     DACENJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.231"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..82)
                     /locus_tag="IW748_002402"
     CDS             complement(<1..82)
                     /locus_tag="IW748_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1105300.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVD"
     gene            complement(325..1635)
                     /locus_tag="IW748_002403"
     CDS             complement(325..1635)
                     /locus_tag="IW748_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1105301.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(1817..3046)
                     /gene="rpsA"
                     /locus_tag="IW748_002404"
     CDS             complement(1817..3046)
                     /gene="rpsA"
                     /locus_tag="IW748_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1105302.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT          880 a          713 c          508 g         1051 t
ORIGIN      
        1 catctactgc tgcagttgca tcttttttag ttaaatcagt agctgaagca actttttcga
       61 ttaattctgc tttgtttgcc atggatagat tcacctcctc cacaagagat atgatagaaa
      121 tatcatttag ttaaaacttt ctgagtggtc cagaagttta aaaagaaata ttgattaact
      181 tcaatattct gttatcacga tatcatatga accgcttaat agcaaggttt ttaccaattt
      241 tcacatgctt tttttagtca tagaaacgta acaaacgaaa ctgtcttttt cttttgaaaa
      301 aaaaacgtcg atatggtaaa atgcctattt tctcctacga gggataattc ggatggacgt
      361 cccttcaaaa gtaaatgctt ttctgatttg attttctaag aaacgttcat aagaaaaatg
      421 catcaattcc tcttcattga caaaaacgac aaatgtaggt ggtttgacag cgacttgtgt
      481 agcataaaaa atcttcaggc gttttccttt atctgttggt gttggattga ttgctacagc
      541 atccataatg atatcattca acacagccga agagatacga agattctggt tcatacttac
      601 ttgttcgatc atttcaggca gacgttccaa ccgttgttta gtcactgcag aaacaaaaac
      661 gattggagcg taatcaagat accggaattc atcacgaatc tcttgttcga aatcacgcat
      721 tgtattggtt tctttcttta caagatccca tttattcacg acgataataa ttccacgacc
      781 agcttcatgg gcgtaacccg ctactcgctt gtcttgttcc cgaatccctt cttcagcatt
      841 cagtaccatc aaaacgatat cagaacggtc aatggcacgc attgcacgca tcacgctgta
      901 tttttcagta ttttcgtata cttttccgcg tttacgcata ccagctgtat ctatcattaa
      961 gaatttctga ccactttcag aaacgaagtg ggtgtcgatt gcgtctcggg tcgttccttc
     1021 aatatccgaa acaattacac gttcttctcc taaaatcgca ttgatcaaag aggatttacc
     1081 aacatttggg cgaccaatca aactaaactt aatcgtgtcg tcgtcctctt cttcagtctc
     1141 ttcagagaaa tacttcactg cttcatctaa aatatcacca atccctagtc catggcttcc
     1201 tgaaacagga taaggatcgc ctagccctaa ggaataaaat tcatagatat cgtttctcat
     1261 ttctggatta tctactttat tgactgctaa aatcactggt ttattacttc tgtataaaat
     1321 tttcgcaacc atctcatctg cgtctgtcac gccttcgcgt ccgctgacta cacaaatgat
     1381 tacatctgct tcttcaatcg cgatttctgc ttgatgcttg atctgatcca taaaaggttc
     1441 atcgccaaga tcaattccgc ctgtgtcgat cacgctgaat tctcgaccta accattcacc
     1501 ttttgcataa atacgatcac gagttactcc aggagtatct tcgacaatcg agatacgctc
     1561 accagcgatg cggttaaaaa tcgtagactt accgacgttc gggcggccaa cgattgcaat
     1621 tgttggattt gccatattat ttcctcctca caagtttcca atctcactta gtttaacgaa
     1681 tgcattgcca actttcaagc atagattgtc aattaaacga agtattttta ttccttattg
     1741 aaatactaat cgcttttcaa aagaaaaact gagacaaaag cagctgcttt catctcagtc
     1801 acagatacaa aatcaattat ttttcagaat catcggttgc tttttcttct acttcttctt
     1861 tcaacgcatc acctaaaatg tctcccatag taaatccagt attttcttct ggtaattcat
     1921 aagactcaac attttttggt tcttcttctg aagctggttt ttcttctaaa gctttgatac
     1981 tcaatgcaat acgatgttct tctggatgga tctctaatac tttcactttc acttcgtcac
     2041 cttcatgaag cacttcatgt ggtgtcgcaa tatgtttatg tgaaatttga gagatatgga
     2101 ctagcccttc tactccaggg aacacttcaa caaaggcacc gaagcttgtc aaacgtttaa
     2161 ctgttccgtc taatactgtg ccaactggag cttttgtatc gatttctgac caaggtccag
     2221 gctgtgtgtc tttgattgac aaggaaacac gttcttgttc aggattgata gacaacactt
     2281 tgacatttac atcatcattg acagccaata catcacttgg ttttgctaca tgactatgtg
     2341 aaatctctga gacatggact agaccgtcaa ttccgcctag gtcgacaaat gcgccaaaat
     2401 cagtaagacg tgcaacacgt ccactgagaa catctccctc atggatagtt gaaagaattt
     2461 cttttttctt ttcttctctt tcgttttcca ctaccgcttt atgcgataag atcaaacgat
     2521 tttctgatgg ttcgatttcg atgattttaa agctcaatgt tttgccttta tagtctgaaa
     2581 agtctgcaac aaaatgatct tccaccattg atgcagggac aaaaccacgt acaccgacgt
     2641 caacgactaa accgcctttg acaacatttg taacaggtgc ttcgatgacc ttgccctctt
     2701 ggaaatctct ttcgatatct tcccaaactt ttttagcatc taaacggcgt tttgaaagta
     2761 ggtagctgcc gttttcttta tctttgccga tcgtactgat aacgactaaa tctaatacat
     2821 caccgacttt tactacttcg ctgatatctt ctaccggcaa agttgataat tcttttgccg
     2881 gaacgactcc ttcaactcca gccccttcga ttccgacaat cacttgtttg tcttcaatga
     2941 ctaaaacttc gcccttaaca acatcgccaa cttttacttc ttgtacgctt ttcattgcgt
     3001 cttccattga ctggttgttc tcttgatttt gatcaaattc tgtcataaca cttgtcctcc
     3061 taaccaacat tctgccgtaa gtacggtact gtttttagtt taattgatta ttcaaaaaaa
     3121 ataaagcgat ttcgttcgtt ttccttgaaa aa
//
LOCUS       DACENJ010000225         3143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.232, whole genome shotgun sequence.
ACCESSION   DACENJ010000225 DACENJ010000000
VERSION     DACENJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.232"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            218..1051
                     /locus_tag="IW748_002405"
     CDS             218..1051
                     /locus_tag="IW748_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1105303.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1153..2853)
                     /locus_tag="IW748_002406"
     CDS             complement(1153..2853)
                     /locus_tag="IW748_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105304.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
BASE COUNT         1113 a          522 c          624 g          884 t
ORIGIN      
        1 ttattaccga gaagcagcag tttgaatact gtttagtgga gaggtcgtga aaaagctgcc
       61 ctgcaccaag tgataaaaac agccaaacaa aaaatagctc ctggtccatt gagcttatta
      121 ctgaggagtt agcaggagaa cactatttgc tggagaggtt gtgacaaaaa ttttgtcaca
      181 acccttttgt ttcgaaatga agacaaggag aaaaccaatg aaattcatga cactcaacac
      241 gcatagctgg cttgagccag aaccagagaa aaaattgcaa gaattagcag ataagatcct
      301 gttagaagat tatgaagtga ttgctttaca agaaatcaac caattactgg aatctgaaga
      361 agtagaacct gcgaagctaa tgaagttctg tccggtaaag aatcaagtac ctatccgtaa
      421 agataatttt gcctaccgtt tagttcagct tttaaaggaa cacggaaaag agtatttttg
      481 gagttgggaa atgagccaca tcggttatga taaatatgaa gaaggaaatg cactgcttac
      541 gaaaaaaaca ttggaaagtc aggtccttac cgtctcccaa tttcaaaaga aagaagatta
      601 tcagacacgc aagatcttga ttgggaaaac aaagatagat gatcaggaca tttttgtctc
      661 gagctgccac ttttcttggt ggacagataa aaaaagtggc ttttattttg agtggaaaaa
      721 cttggaaaac tactttttgg aaactcgcgt accacttttt ttcctaggtg atttcaataa
      781 tccagtagac tctcaaggtt attatactgt aagagaaagt tgccttcttc ttcaagattc
      841 gtatgttgtc gcgaatgaaa aaggaaaagc agctactgtg gaaaagaaaa tagatggttg
      901 ggaacaaaat acagaaaaat tgcgtataga ttttattttc gtccctgaag gaatgcaggt
      961 taaaaaatat caacggattt ttgatgggat cgatagtcca atcatcagtg atcactacgg
     1021 ggtggaaatc gaattagatg tgaacgaata aaaaatataa catataacat aaaaaaactg
     1081 attaaaataa aactctccaa acgattatac cgtttggaga gttttttgat cggatgtatt
     1141 tatccctttt gcctaagcac gtaaaagctt gtatctatga taaataaata aacatagcaa
     1201 ggtaaaccag ccaatcaaag taagagaaag aatgaaatag aaatagattg gcgtttgata
     1261 atgaagcgtg agctcgttga ttcctttttg ctgcttcacg attggggtcc cgatatctgt
     1321 tacttcataa tcagtcaatg tttttccatt ttgctgtagc acagtccgat cgtagacgat
     1381 aacagggata tgaacagttt tgtccccagt gtttttccac ttgattgtca aaagaccgcc
     1441 tggatgtgtt tttataaaag ttgtttcgtt ttctagtatt tcttcgttat accggtcgta
     1501 tttattgtct tttgtctcct tatagattgg cagatagtca ggtgtggttt tttggaaaac
     1561 gaacaagctg gtagacatat ctttgctgaa gaatgttttc cttgcttctt ccggcgtatc
     1621 gaatagataa gtatgtcgac tgacaaatgt ttcattttcc cacttttcca gatgctggta
     1681 taagttctgg atcgtctgtg ctgaaaaaat aagaatgcca aatatactta aaagacggaa
     1741 ccacttttgt tcagaccact ggttcagtga aagggcaagg taaagtagaa gcaatagcgt
     1801 aaatggcacg aaaaatcgaa atggaaactg gatcaaacta ataaaaggca catttttttc
     1861 gatcaatgtc gtccatggaa tcaagctagt agaaagaaaa aagaaaacaa gacttgtgta
     1921 caacacgatt ttttggaata aggtgttctt ttgttgaaag aaaataaaaa gcaaaaaacc
     1981 tgataatatc aatataggaa aggccttagg gtaatggagc caataactgc catccagatc
     2041 tatcgccttt tgactcattt ctctgttaac aaagggagga agaatcttat tcccactgta
     2101 aacaatagct aatcctgccc aaatatttgc tgtcagaaca aaataaagca agaccgactt
     2161 aatcagttga agcagtgctt ttcctttttc agtctggtta aaaaagtaat aaagataaaa
     2221 agggatatac atcaaagcta acatgatcga agacagtaaa tggatctgtg tcataagtcc
     2281 cactgagaca gcgactttga taacagggaa ctttttatga aggacgaaat cagcaatagg
     2341 gatcatacat aaaggataaa aacaagtccc ccagctagtg aatccttggt tgatagacca
     2401 gtattgaata gaatacgtac tcatgaataa aacggctact ggtacactta cccaatttcg
     2461 aacattgaca tatctcagta aaatcagcat agaaaatcct gaaataagat aaagaatgaa
     2521 atttgaaaga atctgataat gaaaccagct acctgcaagc aacacgatca atccttgtaa
     2581 ataagcgaaa aatgggccgt aaacggcatt cacgatcctg ccactttgct ggaatccata
     2641 aagggaagga aaataatgga aatccccata tttaatttgt tgcgcggcat catagaaccg
     2701 attgtaatgg aatggggcat cagcacccaa aattagttta tgtgtgaata aatgaggcat
     2761 gagaaatgcc aaagcaatga aaaaaattgc taaaaatggg ccatattttt tcaattgtcg
     2821 aactcctttg caaactttag ataaattcat catatatcct atgcgagagg taggcaagaa
     2881 attagaaaat tcatgaaaat atttatgaat tttacaagaa aaacataagt gaaagttcta
     2941 aggagagagg atgcgacata agttttgttg catctccttg ttttgaaaca gaatttttct
     3001 taatactcga aacaaagtag ctttttcatg acctctcggt aaacggtgtt caatcagctg
     3061 cttctcggta ataagctcac tagcctagaa aatatgagaa gctattttct gactagcgct
     3121 cttattactc aaagcataac gca
//
LOCUS       DACENJ010000226         3132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.233, whole genome shotgun sequence.
ACCESSION   DACENJ010000226 DACENJ010000000
VERSION     DACENJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.233"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..658
                     /locus_tag="IW748_002407"
     CDS             <1..658
                     /locus_tag="IW748_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1105305.1"
                     /translation="NLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVY
                     AFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGI
                     DPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSG
                     ALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF
                     "
     gene            670..1590
                     /locus_tag="IW748_002408"
     CDS             670..1590
                     /locus_tag="IW748_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1105306.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1631..3088
                     /locus_tag="IW748_002409"
     CDS             1631..3088
                     /locus_tag="IW748_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1105307.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
BASE COUNT         1006 a          548 c          644 g          934 t
ORIGIN      
        1 gaatctgctg atctcagtcg tatttgcaat cattatgagc gagttgagaa ataaacgact
       61 cgttaaagtt tatcaaacga tgtccttact accttatttt ctatcttggg tcattatcag
      121 ttactttgtt tacgcttttt taagtcctga caaaggaata ttcaatcagt ggatcactgg
      181 acatggagga gaagctgtca attggtataa tgaaccgaaa tattggccgt ttatcttggt
      241 gttcattggt acatggaaag ggattggtta taacagcatt atttactttg catctgttat
      301 gggaatcgat ccgacttatt atgaagctgc tatggttgat ggagctagca aatggcagca
      361 gatcaaacat gtgacgatac cacaacttat tccactaatg acgattttaa cgattttagc
      421 cgtaggaaac attttccgag ctgattttgg cctgttttat aacattccta ggaattccgg
      481 tgcattatat gatgtgactc aagttttgga tacgtatatc tacaatggcc tgacatccac
      541 aggagatttt ggtatgacgg ctgctgcagg actttaccaa tcagttgtcg gctttgtctt
      601 gttgatgatc acaaatacga tcgcccgacg ttttgatagc gattctgcat tattctaagg
      661 aggaaaaaaa tgaaaaaaaa gaaagtggag agagtccaag tccgctcttt caataagacg
      721 gctaattttt tctctaatat aatgattgca ctctttgcta tttcctgtat tttgccattt
      781 atatttgtta tcgccatctc ttttaccgat gagtccatat tgtcgactca cgggtacagt
      841 ttttggccag aaaagttcag tatatttggt tatacgtact tgttcgacca gatgcaggat
      901 aaaattttcc aagcgctttt cgtcactgta cttgttactg ttttaggtac actgatcaat
      961 agcacggcta cttctttata tgcttatgcg atttcacggt caaactttcc attccgtcgt
     1021 ttttttaccg ttttttgttt gattacgatg ttgttctcac cagggatggt ggccaattat
     1081 cttgttatga caaatcttct tcagttaaaa gatacgattt gggcattgat cttgccaatg
     1141 gctgtcagtc ccttcaatat tattgtgatg cgcacattct ttaaacgttc ggtctctgat
     1201 tcgattattg aatcggcacg gatcgatgga gctagcgaac tgcgaatatt tgttcagatc
     1261 gttttaccgt tagctgttcc aggtattgcg acaatcagtt tatttgccgc attgggttac
     1321 tggaatgact ggttcaatgc tttactatac atccaagatg ataagctagt tccactacag
     1381 tatcttttga tgaaaatcca aagtaatatt caatatctga cacaaaattc aggagcagga
     1441 agccaattga caggcggatt agcctcagtt ccaggagaat ctgccagaat ggcgatcgta
     1501 gtcatctcga cgttgcctat tgcgatcagc tatccatttt tccagaagca ttttgtcaaa
     1561 ggtctaacaa tcggcggagt aaaagaataa agaaacaaaa atataaatca taaaattagg
     1621 aggaagaaaa atgaagttat ggaagaaact agcctttaca ggtgtttcag cgttgttgtt
     1681 gggaacatta gcagcatgcg gaggttctgg ttctaaagac caggcagaag ctagcaattc
     1741 ggacactttg cagatgtatc aaatcggaga taaaccagat aatttcgatc aattgatgga
     1801 agtagcaaac aaacgaatcg aaaaagagat cggtgtaaaa gtcaatatca attacattgg
     1861 ctggggcgat tacgaaaaga aaatgaacgt catcatttct tccggggaaa attatgatat
     1921 cgcctttgcg aacaactatg tttccaatgc ccaaaaagga gcctttgctg atttgactga
     1981 attagcgcct aagtacgctg aaaaagctta taatgattta gatgaagcat atattaaagg
     2041 aaatctggtc aatggaaaat tatatgcttt cccagtcaat ggaaatgtct ttgctcaaca
     2101 agtacttact tttaacaaag ctttactaga taaatacgat ctttcaattg atggaatcca
     2161 atcttacgct gatgcagaaa aagtcttgaa ggaattccat gaaaaagaac caaacacggc
     2221 agcttttgcc attggacaag gcttcaaagt ccaagggaac tttgattatc ctttagggaa
     2281 tacgttacca tttgctattg atctggatgg agatgattcg aaaatcatca accaatacga
     2341 taatgaaacg tatatcggac tgttacgtac aatgcatagc tggtacaaac aaggactcat
     2401 tccaagtgac gctgcaacga gcaataagga ttatccacta gaaggaaata cttggctaat
     2461 gcgtggagaa acacaaggac cgtatgatta tggcgataca attttgacaa atgcagcaaa
     2521 acaagaactt gtctcaaaag caattactgt tccactaaaa tctacagctc aagcacaaat
     2581 ggcaaatttt gtcgtatcaa atacttcaaa aaataaagaa aaatcagtag aacttctagg
     2641 gctgcttaac agtgatccag aattattgaa tgggcttgtt tgggggatcg agggagaagc
     2701 atgggaaaaa gtcgatggtt cagatgataa aatcaaattg ctagatggtt atcagccaaa
     2761 tacacatatg tctgcttgga atacaggaaa caacaagatt ctatatacac aagagtctat
     2821 tacggatgac atgattgcca aacgtgatca atcgattgct gatgcagaaa cttcaccgat
     2881 tttaggattc agcttcaaca ctgacagtgt caaaacagaa ttaagtaata tttctaatgt
     2941 catgaatcaa tatttagatg gattgaatac agggactgtt gatccagatg aaacattacc
     3001 gaaattgaaa gacgctttgg acaaggctgg ctatgataag gtattgaaag aaatgcaaaa
     3061 acaatatgat gagttccgac aagaataata atgaataaag acaaaggggt tgtgacaaaa
     3121 gacgtgtcac aa
//
LOCUS       DACENJ010000227         3131 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.234, whole genome shotgun sequence.
ACCESSION   DACENJ010000227 DACENJ010000000
VERSION     DACENJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.234"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            110..445
                     /locus_tag="IW748_002410"
     CDS             110..445
                     /locus_tag="IW748_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105308.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            442..1059
                     /locus_tag="IW748_002411"
     CDS             442..1059
                     /locus_tag="IW748_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105309.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
     gene            1075..1542
                     /locus_tag="IW748_002412"
     CDS             1075..1542
                     /locus_tag="IW748_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105310.1"
                     /translation="MLKHCRKGFCKVIKGKNAADLIFEWKESNLINVRGKNGSGKTER
                     VMASIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYEEVLFVWITEEDSNSKSAFFSEKNAFLHSI"
     gene            1558..>3131
                     /locus_tag="IW748_002413"
     CDS             1558..>3131
                     /locus_tag="IW748_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAQ1105311.1"
                     /translation="MIVSKKLARNLVSEGYDLRLVEKYQPSGGIKFDERYISTGDGYV
                     TSIAVIEFAEDVNSRWLTDIMAIPNTIATFDVCTADKEDLLHKINTSIGELDYRSTNE
                     NRTTDKTDALLDKYNLEDFAQEITKRGEVVKTVHARIFVYSDSLEELEKRISEIRKDL
                     ASVNHKAIVPQFRMKDEWMSLFQDGMEQESKLFAKKGLTVPAKNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFYSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVKKAGGVYVDLGGGDMVVNPLQIYATVMKKDQFGNDVVDESKSFQ
                     NHITKCSNQIRFLEPEIDTATVREYTLMLQDFYIHYGILPKEWREARDDNERNQLNTN
                     ICNLPNDSYPIYGEFLKFIENQQFKIKNATPERVRSIELIKLIVTDMCTTYPQIFNGV
                     TTMPNFDTTKIVYYNIDSISKMSAPVFHCQLFTALTTIWNSALQNGKIQKENFKKYGS
                     DYYKYAQDFMVLIDEC"
BASE COUNT         1163 a          399 c          603 g          966 t
ORIGIN      
        1 caagacgaag attcacttat gaagacaacg aattattcag agatacactt aatgatccta
       61 atccactttt cgataattta aattaaatta aaagccggca tgagtgatta tgccggcttt
      121 ttatgaggtg aaaatgatgt atagaaatcc caaaaatata aaacaagaga taaagctatt
      181 tttcttctat ttagtcgatt taggaatagt agccggaagc gtgatggcaa gtgtttactt
      241 tgcaaaatta atgccaattt caccaatgat gaaatttgca ctgtacttat tagcatttgt
      301 agtgggtgtt tggatgtgcg ttagaacgcc taatcaccct aaagagcgaa atattttaat
      361 gctgtattat ctctttaaaa tggatagaaa cagataccac gaagtagagt ttaaaagaaa
      421 tagtaggggg aaaattgaat tatgattgtt gctgaaagta atgaaaataa attaagtggt
      481 gtttttgaca aattcttaga actagttatt ccaaaagata aagatatgtg tgaactttta
      541 ccatataaaa aaatattgaa agacggaacg ttagtgacta atgacaataa gtatcaagcc
      601 ttttttaaag ttaaaacaac tgacttaact tctatttcag aagatggatt tatgcgtatg
      661 gtaaacaaac tcacttattt aaatcgagta tatttagaac catataaaat ttattcatta
      721 acttatccta ctgaaacagt aaatcaagcg atttattgga agcatagaat acaggaggca
      781 aagagaagtt tattgagtaa taatgatccc aatttagaaa cgtatctgaa gtggaatcta
      841 aagctagctt acgataatta tgaaagagtt gatctagtat cgactttacc agagttgatg
      901 ttcgttattt gtgtttatgg aaaaacacaa aaagaagtga aaaatcatgc cgatgtaatg
      961 aaacaggccg gtggtagaga attagattta caaagaatta ctgaagggga tttaatcaat
     1021 gtgataacta aattgatgaa tcccaatact tctttataag gcggtgctta ttatttgtta
     1081 aagcactgta gaaaaggatt ttgtaaagta attaaaggta aaaatgctgc tgatttaatc
     1141 tttgaatgga aagaatctaa tttaattaat gtacgtggga aaaatggaag cggaaagaca
     1201 gaaagagtga tggctagtat tgataattca aggcctagat caagagcatt agtaataact
     1261 gatgaaaaaa atcactataa aggcatacga acgatagaaa aaaaatattt aactaacaat
     1321 gatttaaaag tagtagatga tattttaaat atttatcctg attttaattt agttgttttt
     1381 gatgaaacaa ttcctgtgac agaaataaaa ggattctatc aaagcttttc caatctagca
     1441 aataaatatg aggaagttct gtttgtttgg attacagaag aagatagtaa tagcaagtca
     1501 gcgttcttta gtgaaaagaa cgctttttta cattctattt aaaaaggaga attgaacatg
     1561 attgtaagta aaaaactagc aagaaatcta gtaagtgaag ggtatgactt aagattagta
     1621 gaaaagtatc aaccgagcgg tggaattaaa tttgatgagc gttatatttc tactggtgat
     1681 ggatatgtaa cttcgattgc tgttattgag tttgcagaag atgtaaatag tcgatggtta
     1741 acggatataa tggcaattcc aaatactatt gcaactttcg atgtatgtac ggcggataaa
     1801 gaagacttat tacacaaaat caatacgtct attggagaat tagattaccg atcaacaaat
     1861 gagaatagaa caactgacaa aacagacgct ttactagata aatacaattt agaagatttt
     1921 gcacaagaaa ttacgaaacg tggtgaagtt gtcaaaactg ttcatgctcg tatttttgtt
     1981 tatagcgatt ctttagaaga attagagaaa agaattagtg agattcgtaa agatttggct
     2041 agtgtcaatc acaaggcaat tgttccgcaa tttagaatga aggatgaatg gatgtcttta
     2101 tttcaagatg gcatggaaca agaatcaaaa ttatttgcca agaaaggctt aactgttccg
     2161 gcaaaaaata ttggtggcgg tattccattt caccacaaga gtttgaaaga tccacgtggg
     2221 ttttatttag gaactacgtc aactggtggt gcttttatat ttgatccatt ttattctacg
     2281 aagcaaagac ggtcgtttaa cggctttatt ttaggaaaga tgggttttgg taagtcaact
     2341 ttaattaaac agctacagga aggcctacaa gcgaaaaatt gttttattcg aggatttgac
     2401 aagtcaggtg aatttgaagg catggtgaag aaagcaggcg gtgtatacgt cgatttaggc
     2461 ggtggtgata tggtagtaaa tccattgcaa atttatgcaa cagtcatgaa aaaagatcaa
     2521 tttggaaatg atgttgtaga tgaatcaaag agttttcaaa atcatatcac taagtgtagt
     2581 aatcaaattc gctttttaga acctgaaatt gatacggcaa ctgtacgtga atatactttg
     2641 atgttacaag acttttatat tcattatgga attttgccaa aagaatggag agaagcgaga
     2701 gacgacaacg aacgaaatca attaaataca aatatttgta acttgcctaa tgattcatat
     2761 ccaatctatg gagagtttct taaatttatt gaaaatcaac aattcaaaat taaaaatgct
     2821 actcctgaac gtgtccgttc tattgagcta attaagctta tcgttacgga tatgtgtaca
     2881 acttatccgc aaattttcaa cggagtaaca acaatgccaa actttgatac aacaaaaatt
     2941 gtttactata acattgattc tattagtaaa atgagtgccc cagtattcca ttgccaatta
     3001 tttacagcgt taacgactat ttggaacagt gccttgcaaa acggaaagat tcaaaaagaa
     3061 aactttaaaa aatatggcag tgattattat aagtatgcac aagatttcat ggttttgatc
     3121 gatgagtgtc a
//
LOCUS       DACENJ010000228         3109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.235, whole genome shotgun sequence.
ACCESSION   DACENJ010000228 DACENJ010000000
VERSION     DACENJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.235"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2177)
                     /locus_tag="IW748_002414"
     CDS             complement(<1..2177)
                     /locus_tag="IW748_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105312.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3109)
                     /locus_tag="IW748_002415"
                     /pseudo
     CDS             complement(2849..3109)
                     /locus_tag="IW748_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          859 a          696 c          508 g         1046 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttt
//
LOCUS       DACENJ010000229         3100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.236, whole genome shotgun sequence.
ACCESSION   DACENJ010000229 DACENJ010000000
VERSION     DACENJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.236"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..282)
                     /locus_tag="IW748_002416"
     CDS             complement(<1..282)
                     /locus_tag="IW748_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAQ1105313.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIF"
     gene            complement(626..2278)
                     /locus_tag="IW748_002417"
     CDS             complement(626..2278)
                     /locus_tag="IW748_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1105314.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            complement(2338..2917)
                     /locus_tag="IW748_002418"
                     /pseudo
     CDS             complement(2338..2917)
                     /locus_tag="IW748_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
BASE COUNT          976 a          731 c          523 g          870 t
ORIGIN      
        1 aaaaatttta aataacaaaa catgagacaa atatactttt gcagcattta aatcttgtgt
       61 catataaggt agtaacactg ataccgtaat tacgataata tacggaatat aaatcttgat
      121 aaatcgtttc tgtaaaaatt cacccaatgt aataggcttt tttaaatatg acagatatag
      181 cccaaaacct gagcaaaaga agaatatatg cactcctgta tcaccaatag atattgcttt
      241 gttcagtatt gctggcaaat acgattgctg aaaatagtac atcaatacaa tagcgaatat
      301 tgaaaacgcc ttctcaaaac taataatatc acgtttattt ctttccccct cattttatta
      361 tttcaatagt tttattcata ttctactcct ttcatctcta ttgctgaata cattattaaa
      421 actctaatat ctcttatttc tcccttactg ctttgatcgg tgcttcatca ggctgctgtg
      481 tctctgttaa cacttctttg ccttttcgat ccacacagtc ggccccttta cctcaattat
      541 gtaaacagaa ccgtaaagca ggaaattttt attaaacata gatgttgcta cttcggacat
      601 tgacactcag atctttttca tagtattact taaacatttc agccagttca gcagccataa
      661 cagcttccat tacttcggca gcttccttgc tttctgcgct aacgctgata taggtcttca
      721 gcttcggctc agtgccggaa ggccgaacca ctacagagca gttgccctcc agcagatatt
      781 tgagaacatc agacttcggc aggccattca gtcccgtgct gtaatccaag gtttcaatga
      841 ccttcttacc tccaatcata tcaagacctt tatggaattc aaccataatt ttctgcatct
      901 tggtaaaacc tgccgcaccc tcaaactcaa aggaatgcag ggtgttcaaa cagtagccat
      961 acttagcata aatctcgttc agcttatcaa acagactgat accctgagtc ttgtagtaag
     1021 agaacatctc gcagatcaga taagcggcgt tgacaccatc cttgtcacgg acatagctgc
     1081 cggatagata gccgtaggat tcctcaaagc cgaaaatata gctgtccacc ttgcctgcct
     1141 tttccagcat accaatctgt tcgccgatga acttgaagcc ggtcaacaca tcgacagtac
     1201 gcacgccata gttggaagcg atccgctcgc ccagatccat agtcacgatg gtcttgacca
     1261 tcacagggtc agtgggcatc ttgccgtgct taattctctg agagcatatg tagtccagaa
     1321 gcaacagacc agtttcgtta ccggacagca ggacataatc cccttctgca ttcttcacgg
     1381 cgatacccac acggtcgcag tcagggtcgg tagccagcag cagatcagca ttatacttct
     1441 tggcatactc catgcccaga gacatagctt cattaatttc cggattcgga taaggacagg
     1501 tcgggaaatt accgtctggc agacgctgct cttcgacaac ggtcacattg ctgtagccca
     1561 tttctgccag agtacgggta acaggcacca gaccggtacc attcaaagga gaatacacga
     1621 tagcaacatt cttgtccact tcttcgccaa acagaaccga ctggcccttt acttcttcaa
     1681 tgaaagcagt cagaacctct ggctggatag aagcgattct ggaagcgtcg ccgcccattt
     1741 tcacatctgc aaaaatatcc agcttttcaa tttcagccag aatctcagcg gcagcctcgg
     1801 tggtgatctg gcagccgcct gcgccataga ccttatatcc gttatacttg gaaggattat
     1861 gggatgcggt gatcataaca ccggcagaag ccttcagata acggatagca aaggatacgg
     1921 taggcacagg cagcagctcc ggccacagat gcacctgaat accgtttgca gcaaagatgt
     1981 ccgcggcaac ctttgcaaat acatcggact tgattcggga atcatagccc acgacgacag
     2041 aaggggtttc ataggtctta ttcagatagt ctgacagtcc ctggcttgcc ttacctacgg
     2101 tgtgtacgtt catacgatta gtgcctgcac caatggtgcc acgcagacca ccggtaccaa
     2161 aggacagatt tttatagaag gagtcctcaa tgtccttctc ggaatatgac cgcagttcct
     2221 ctacaatttc tgcattggca tttgtaagcc aatgataata atttgcatta tgatccatag
     2281 aatctctcca atcttttaaa attctgctca tttattttta aggttaggat gaatatatca
     2341 gctcaaataa tatataaccc cactaatgcc gccacccaaa gtattataaa ataaatctga
     2401 caattggaat gtctctatgt gtaaaaataa ctgcaaaaac tctataagac acgaacataa
     2461 aaaaacaaat tttataatct ctgataaatt tttccaaaat ctttttctga agccttatac
     2521 cacattacta gaattataaa tggaataaac agaataacat tttcaaaaca ttctgtagtt
     2581 aatctaatct cccctgtcgt ttcattcaca gagtaaatcc accaactacc aaacacatta
     2641 gacaaaggat ttagccaaac atatctatcc atcagtgttc taaaaagaat catatttgta
     2701 aaaaacacaa gcaaaaacat ttttctaaat gagcttgatt ttctgaaata ccgaatccag
     2761 acaagaaaag attttttcaa acctaatcct gatttctcct cagccatcgc aaacaagtat
     2821 aaaaacatag ccaaaactgc aattaaaatc gaaacccaaa tatttgatac aatgcagata
     2881 acacattaac caaaatagaa gatatgattt tcttcataaa ctctcctttc atcatgcgct
     2941 aaatgagtac catcaagaag gtttcgtaaa aggaggtacc cactctaaca tgaataacct
     3001 cggccagcga aaataaaact caacagaatt cctatctcta tgctgctata atttcaacaa
     3061 atttacatgt accagttact aatctaatat aattttttca
//
LOCUS       DACENJ010000230         3021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.237, whole genome shotgun sequence.
ACCESSION   DACENJ010000230 DACENJ010000000
VERSION     DACENJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.237"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..807
                     /locus_tag="IW748_002419"
     CDS             <1..807
                     /locus_tag="IW748_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1105315.1"
                     /translation="NRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQ
                     PEKIEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILV
                     NDRESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGD
                     YSRKSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKN
                     DLSILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            839..2236
                     /locus_tag="IW748_002420"
     CDS             839..2236
                     /locus_tag="IW748_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1105316.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            2395..3018
                     /locus_tag="IW748_002421"
     CDS             2395..3018
                     /locus_tag="IW748_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105317.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1084 a          487 c          646 g          804 t
ORIGIN      
        1 aatcgaagct tctctattgg acttttgata aacgggattt ccagccgttt tgggtcggaa
       61 gttgtctcag ggatcgaaag agtagctgaa gaaaatgggt atagcttatt attaagcggg
      121 tttgccgatc agccagaaaa aattgaacaa aaaattgaat acctgatgaa gcatgtgatc
      181 gatgggctga ttgtcttctt atccgaagaa gagtggtctg gatttgaaat gctcacaaaa
      241 atgagtattc cagttatcgc attgaattgt ccagaaggac cagcaggtat tgatacaata
      301 ttagtgaatg atcgagaaag tgtagcaaaa gtgatcgctc atcatggtga acaaggacat
      361 aaaaaaatcg gatttattgc tgccactcaa acagactacg tagcaagaga acgtttagca
      421 ggagccaaag aagcggtcca aaatgatcca tcgatccaat tagaggtctt tacaggagac
      481 tattctagaa aaagcggcta ttacggggca aaagcattat tagaaaaagg ggcaactgct
      541 atttttgtca gcaattacaa tatgtcgatt ggtgccatcg aatgtttcaa tcaagaaggt
      601 gtcagaatcg ggaaggatat tgcgttcagt cactatgatt atttagataa aaacgatctg
      661 agtatccttc cgaaaatcac gatcacgcca ccaacagata aaatcgggga acgagcagca
      721 gaacagctgt taaaacgaat caaaaaagaa gaggaagaaa atgaaaaagt gattgtgatg
      781 gaaaatatca tacatggaat caactaaaac gaactaaaaa agaaaaagag gtgctttgat
      841 gaaaaaatat caatttccag caaatttttg gtggggttct gcagcaagtg gcccacaaac
      901 agaaggaagg atttcaggcg atggaaaagg agagaacatt tttgattatt ggtacaaaaa
      961 agaaccgcaa aagttctttg atcaagtagg acctgaaaaa acctcgcaag tttatacgaa
     1021 gtatcaagag gatgtacagt tgatgaaaca gactgggcat aattcatttc gaacatctat
     1081 ccaatggagc cgtttgatac ctgatgaatc tggaacaatc aacgaagaag ccgttcgttt
     1141 ctatcatcgt tattttgatg agttgattga aaacggtatc gaaccctttg tgaatctgta
     1201 tcatttcgat atgccgatgt atctgcaaga aaaaggcgga tggttgaacc gagaaacggt
     1261 tgaagcctat gaaaaatatg ccaaaagttg ttttgaattg tttggttcga aagtaaaaaa
     1321 atggtttact cataatgaac caatcgttcc agtcgaagca ggttatttat atcgatggca
     1381 ttatccagaa gagtcagata tgaaaaaagc gatccaagtt ggctatcatg aagcgttggc
     1441 ttcagcgtta gccatcaaat cttaccatga aatggagcaa ggtggtgtta ttggtatcat
     1501 cttgaatctg acaccaagct atccaagaga cgaatcaaac cctgaagatg ttaaagcagc
     1561 gcaaatagca gatgcgttct tcaatcgttc attccttgat ccagctgtaa aaggcgagtt
     1621 tccaagtgaa ttgattgaga tagtaaaaga acttgatctc atgcctgtga tccatgcaga
     1681 ggatttgacg gttatcaaag aaaatacagt agatctttta ggtatcaatt attaccagcc
     1741 acgccgggta aaagcaaaag aaacaccaat cgatactaaa aacggtccaa tgcctgagga
     1801 ttatttcgac aattatgaaa tgcctggtcg taaaatgaat ccttatcgcg gatgggagat
     1861 ctatgaacaa ggaatctatg atattttgat caatgtacga gatcattatg gaaatatcga
     1921 ctgctttatt tctgaaaacg gtatgggtgt agaaaacgaa gcacgattta tcaaagaaga
     1981 tggcatgatc cacgatgatt atcggatcga atttgtccaa tctcatctga aatatgtcca
     2041 tcaagccatt caagaaggtg ccaattgttt aggctatcat atgtggacat gtatggataa
     2101 ctggtcgtgg accaatgcat ataagaatcg atatggcttt atttcagtag acttagcaca
     2161 agatggaaaa agaacaatca aaaaatcggg tcattggttc aaagacgtat cagataaaaa
     2221 cggatttgag gcttaagaaa gaatagttga gagcaaatgc gtgaaattct ccattcttac
     2281 cattttgtga aaaagaagtt caattattta cgatcttttt caactttttt gtctttctat
     2341 ttcatttttt gtatataata gaatagtcaa aattaaatag gagtggagga aagcatgagg
     2401 aaaaaaagac cgtttcttct cttttggtta ttcggttttt tcttactgct tattttatcc
     2461 gcagcatttt caggaaaaat gacagcagat cttgctcaga aagaaacaga aaaaatcgta
     2521 gcaagcaaag aatcagtaga taatttcaaa aaagaattct caacttattg gtgggcggag
     2581 tataaaataa ctgccaatta cgaaaaaggc gaattggaca tttatttgcc aaaaggaaaa
     2641 gaagaaaatc aatttattaa tatcgatgac gtacttagtg cgatttctct cagacaatat
     2701 caatttaaag acaagcattt gaccgttttg cttttgactc atgatggcga caaagtagcc
     2761 cgtttgaaaa atggaaaagt gatcaccgct tattctgaaa aagtagacaa aaaagtggtc
     2821 gccgatcgtt tagcggaatg gaaaaagaca aatgaacaag tcaagaaaac aactgtctct
     2881 tctcaaaaaa acgagtcgac aggaacaagt ccttcagatt ctggcagtgg acaaacgata
     2941 acgggaagtg aagaaaatca agcctcaatt attacgaatg agtcatcaca aggacaatat
     3001 caatcctatg gacaatgaaa a
//
LOCUS       DACENJ010000231         3015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.238, whole genome shotgun sequence.
ACCESSION   DACENJ010000231 DACENJ010000000
VERSION     DACENJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.238"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(86..718)
                     /locus_tag="IW748_002422"
     CDS             complement(86..718)
                     /locus_tag="IW748_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1105318.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(725..1792)
                     /locus_tag="IW748_002423"
     CDS             complement(725..1792)
                     /locus_tag="IW748_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1105319.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(1789..2520)
                     /locus_tag="IW748_002424"
     CDS             complement(1789..2520)
                     /locus_tag="IW748_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1105320.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(2689..>3015)
                     /locus_tag="IW748_002425"
     CDS             complement(2689..>3015)
                     /locus_tag="IW748_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1105321.1"
                     /translation="SAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTF
                     IELPDGEEEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKI
                     VKVEQA"
BASE COUNT          874 a          644 c          459 g         1038 t
ORIGIN      
        1 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
       61 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
      121 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
      181 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
      241 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
      301 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
      361 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
      421 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
      481 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
      541 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
      601 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
      661 aagaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
      721 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
      781 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
      841 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
      901 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
      961 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
     1021 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
     1081 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
     1141 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
     1201 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
     1261 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
     1321 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
     1381 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
     1441 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
     1501 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
     1561 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
     1621 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
     1681 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
     1741 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
     1801 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
     1861 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
     1921 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
     1981 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
     2041 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
     2101 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
     2161 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
     2221 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
     2281 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
     2341 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
     2401 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
     2461 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
     2521 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
     2581 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
     2641 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
     2701 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
     2761 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
     2821 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
     2881 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
     2941 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
     3001 ttcatctttc gctga
//
LOCUS       DACENJ010000232         2999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.239, whole genome shotgun sequence.
ACCESSION   DACENJ010000232 DACENJ010000000
VERSION     DACENJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.239"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            347..2944
                     /gene="adhE"
                     /locus_tag="IW748_002426"
                     /gene_synonym="adhC"
     CDS             347..2944
                     /gene="adhE"
                     /locus_tag="IW748_002426"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1105322.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1067 a          540 c          634 g          758 t
ORIGIN      
        1 acagaaacgc tggaagagac agaagagaag gaagaaaaat aagtatcaaa gcttatttaa
       61 agacgaggta gggcagaaag atgtcctacc tcatttttta ttcaaaaaag aaagagataa
      121 gagcatggga tatattgctc aatattgaat aatcgggtgt gtcatataaa taataataaa
      181 taactttata tatttttcta tacaaacacc tgtaaataaa gcaattttat atttaaaaat
      241 aactgtgaaa aaaatcacaa aaaagtgttt acatagaaaa ataaatattg tatgatatac
      301 atgtgaatta tttaacaaat aaccatgacc cattaggagg ataatgatgg ctaaagtaat
      361 agaaaaagaa aaaacgaaga caattgacgt acaagcaatg attgatgatt tggcagaaaa
      421 agcaaatgca gcattgaaag aaatggaaag ctttgatcaa gaaaaagtag accatatcgt
      481 gcatgaaatg gcaatggcag ctttggatca acatatgcca ttagcgaaga tggctgtaga
      541 ggaaacaggc cgcggtgttt acgaagataa agcaatcaaa aatatgtatg cgtccgaata
      601 tatttggcat aatatcaaac atgataaaac agttggtgtg atcaatgaag atgtccaaaa
      661 agggttgatt gaaattgctg aaccagtcgg tgtggtttgc ggagtaactc caacaacgaa
      721 tccgacttcc acaacgattt tcaaatcaat gatcgcgttg aaaacaagaa atccaatcgt
      781 ttttgctttc catccaagtg cccaaaaatc ttctgcagaa gcagcacgag tggtacgtga
      841 tgctgctatc gctgccggag ctcctgaaaa ttgtatccaa tggatcgaac atccttcgat
      901 cgaagcgact tctatgctaa tgaaccatcc aggtatcgcg atcgtcttag cgacaggtgg
      961 agctggaatg gtcaagtctg cttactctac aggaaaacca gctttaggtg taggtccagg
     1021 taacgtacca gcttatattg aaaaaacagc aaaaattaaa cgtgcagtga acgatttgat
     1081 cgtttcgaaa acatttgata atggaatgat ttgtgcttct gaacaagctg tgatcgtgga
     1141 taaagaaatc tatgcagcag taaaagcaga attccaagca caccaagttt atatcgtaaa
     1201 accagatgaa ttgcaaaaat tagaagatgc agtgatgaac gaaggaaaat atgcggtcaa
     1261 tccatcaatc gttggacatt ctgcgatgga aatcgcaaaa ttggcaggaa tcagtgtacc
     1321 aaaaggaacg aaaatgctga ttgctgaatt ggaaggcgta ggcccagatt atccgctatc
     1381 aagagaaaaa ctttctccag ttcttgccat gatcaaagct aacaacacgg accatgcgtt
     1441 tgatttgtgt gaaggtatgt tgaatcttgg aggattagga catacagcag ttatccattc
     1501 agaaaacgaa gagttgcatg tcaaattcgg tcttcgaatg aaagcttgtc gtattttagt
     1561 gaatacacca tctgcagaag gcggcatcgg tgatatctat aacgaaatga ttccgtcatt
     1621 gactttggga tgcggttctt atggtaagaa ctctgtttct cgtaacgtct ctgctgtaaa
     1681 cttaatcaat gtcaaaactg tagcgaaacg gagaaataac atgcaatggt tcaaattacc
     1741 gccaaaaatc ttctttgaaa agaactctct attgtactta gagaaaatgg aaaacgttga
     1801 acgtgtcatg atcgtttgtg accctggtat ggtccaattc ggttactgcg atacagttcg
     1861 tgaagtattg gcacgacgta aaaatgatgt gaagatcgaa gtcttctcag atgttgaacc
     1921 gaacccatcg acaaataccg tatacgcagg aacgaaactg atggcagact tcaagccaga
     1981 tacagtaatc gctcttggag gcggttctgc aatggacgca gctaagggaa tgtggatgtt
     2041 ctatgaacac ccagacacat ctttctttgg tgcaaaacaa aaattcttgg atatccgtaa
     2101 acggacatat aagatcgaca aaccagaaaa aacacaattc gtatgtatcc cgacaacatc
     2161 agggactggt tctgaagtaa cgccatttgc ggtcatcact gacagtgaaa cacatgtgaa
     2221 atatccgtta gcagattatg cattgacgcc agatgtcgca atcgtcgatc ctcaattcgt
     2281 catgtcagtt ccagcatctg tcacagcaga taccgggatg gatgtgttaa cacatgcaat
     2341 cgaatcctat gtttcagtca tggcttcaga ttatacacgt ggattgagcc tacaagcaat
     2401 caagttagtc tttgactatt tagaaaaatc agtaaaaaca cctgatatgg aatcacgtga
     2461 aaaaatgcac aacgcttcta caatggctgg tatggcgttt gctaacgcat tcctaggtat
     2521 ttgtcactca gtcgcacata aaatcggcgg ggaatacggg atccctcatg gacggacaaa
     2581 tgcgattctt ttaccgcata ttatccgcta taatgcaaaa gatccacaaa aacatgcgat
     2641 gttccctaaa tatgactatt tccgtgcaga tacagattac gcagatatcg caaaattctt
     2701 aggtctgaaa ggcgaaacaa cagaggaatt agtcgaagct ttagcaacgg ctgtttatga
     2761 attaggaaaa tctgtcggca tcaacatgag tctaaaagct caaggagtca cacaagaaac
     2821 actagataca accgtagacc gtatggctga attagcttac gaagaccaat gtacgacagc
     2881 taatccgaaa gaaccattga tctctgaatt gaaacagatc atcattgatg cttacaacgg
     2941 ataagcaaaa gaaagcgtag aaaaaagata agaaaataga tgaaaaagaa cttggaaaa
//
LOCUS       DACENJ010000233         2968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.240, whole genome shotgun sequence.
ACCESSION   DACENJ010000233 DACENJ010000000
VERSION     DACENJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.240"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(84..1031)
                     /locus_tag="IW748_002427"
     CDS             complement(84..1031)
                     /locus_tag="IW748_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1105323.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1277..1741
                     /locus_tag="IW748_002428"
     CDS             1277..1741
                     /locus_tag="IW748_002428"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1105324.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1803..2846)
                     /locus_tag="IW748_002429"
     CDS             complement(1803..2846)
                     /locus_tag="IW748_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1105325.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          981 a          587 c          567 g          833 t
ORIGIN      
        1 tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa
       61 atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt
      121 tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccaata
      181 tatttcccga tgattccaag acataacaat tggattccac caatgaacag gaagatcgag
      241 accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac
      301 aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac
      361 ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac
      421 caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaat
      481 ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca
      541 tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg
      601 ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg
      661 ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg
      721 atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac ggtcactaaa
      781 tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa atttctggaa
      841 aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta
      901 ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt
      961 tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt
     1021 gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta cattcaaaat
     1081 ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac aggtattctt
     1141 tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca
     1201 atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctattg
     1261 actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga
     1321 tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca
     1381 gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga
     1441 atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc
     1501 tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg ttgctgttga
     1561 tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat tgccaatcgt
     1621 tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc
     1681 aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta
     1741 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
     1801 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
     1861 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
     1921 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
     1981 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
     2041 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
     2101 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
     2161 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
     2221 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
     2281 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
     2341 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
     2401 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
     2461 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
     2521 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
     2581 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
     2641 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
     2701 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     2761 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     2821 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     2881 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     2941 aaagtcttgt acgacctctt gtttcgaa
//
LOCUS       DACENJ010000234         2957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.241, whole genome shotgun sequence.
ACCESSION   DACENJ010000234 DACENJ010000000
VERSION     DACENJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.241"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..263
                     /locus_tag="IW748_002430"
     CDS             <1..263
                     /locus_tag="IW748_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ1105326.1"
                     /translation="VRHESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLF
                     SDNHEVEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            263..880
                     /locus_tag="IW748_002431"
     CDS             263..880
                     /locus_tag="IW748_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105327.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            884..1333
                     /locus_tag="IW748_002432"
     CDS             884..1333
                     /locus_tag="IW748_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105328.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            1333..1827
                     /locus_tag="IW748_002433"
     CDS             1333..1827
                     /locus_tag="IW748_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105329.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     ESVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            1841..2272
                     /locus_tag="IW748_002434"
     CDS             1841..2272
                     /locus_tag="IW748_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105330.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            2274..2411
                     /locus_tag="IW748_002435"
     CDS             2274..2411
                     /locus_tag="IW748_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1105331.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            2448..2741
                     /locus_tag="IW748_002436"
     CDS             2448..2741
                     /locus_tag="IW748_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105332.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            2738..>2957
                     /locus_tag="IW748_002437"
     CDS             2738..>2957
                     /locus_tag="IW748_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1105333.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT         1075 a          434 c          636 g          812 t
ORIGIN      
        1 gtgtacgcca tgaatcgatt tctgaacgtt tcctatccga tctaaattat ttgtatcaaa
       61 aaatgaaagc agcacttttc acgattgaat acggtgaaat tgaagtgact gatattcttc
      121 aagacgatct cttttcagat aatcacgaag tcgaaaaaat tgaaacagta gaatttccaa
      181 ttgaagaagg agctttgatt attgcaactg ttgatgatcc aaaacagaat gttttcactc
      241 ttgagaaagt tggggtgatt tgatggttaa agtaaagaga atgatggaga ctgatgaatc
      301 tggtgttaaa cgtcaattta tgccaattac acatgtttct gcagttttag gcttagaaaa
      361 aatgatatca ggacaatcaa aagttctttc ggttaatggt aaatatggtg cagtcatttt
      421 aacgaaagct gatttaggat tagagaatgc aattactgaa ctaccttatg caagcgagac
      481 aagcgatggt attcttactg ctgaaatgtt tcaaaagatc gtaaatggag aaggtggaac
      541 atatattttg ccgattgcta ctcctgaaca attaggaggt ataaaagttg gggaactctt
      601 agaagttact gaagaaggtg ttttatctgc aactaagcaa acagacttca atttctctga
      661 agaattaaag ggaaaattag aatctctaaa aatattaaaa gcaggtgcaa atatatcaat
      721 tgcagaagat ggaacaatta gttctacagg tggtagtggg actggtggtg ttaatcagtc
      781 atatgtagat caaaaattcc aagaagctgt aaatcaagca gaaaattaca caaacgaaag
      841 aattccaaat tttacttttg aaaaaatcgg ggaggtatag aaaatgacag atatcgtaaa
      901 aataaaacaa agcggtgtac aggtttatcc acaaactcat tggaatgcta tagaaggcaa
      961 accaacaacg gtgaagggag ataaaggcga tcctggccaa gcagcaacaa ttaccatagg
     1021 aaccgtttct agtggttcaa ccgcttctgt tacgaatgta ggaacatcat ctgcagctag
     1081 atttaatttt gttttaccaa agggagataa aggtgatcct ggaataaatg ccacaactac
     1141 agctgtagct acaactacag caaatgggtt gatgtcgtcc acagataaaa ctaagttgga
     1201 tggaattgca gctggagcac agaaaaatcc aggtaatgct acaactacga cagcaggttt
     1261 aatgtcagca accgataaag tgaaacttga tggattagcg aatattacat ttgagaaagt
     1321 ggggacggtt taatggctga tattgttcaa ttaaaagaag acggagttgc taaatactta
     1381 aaaacgcatg tagaagctat tgatggtaaa gaagcattgg tgcagacaga tggagaccaa
     1441 gccattgcag gacataagaa tttttcaggt tctgtaacaa tcaataataa acgtgttttg
     1501 acgactgatg ataacagata tgaagtggta aatctggttg tcactaacgg taatacaggg
     1561 acagcaaagc tttatcgtga aggaaaaaca gtttctatat attttgtggc tttaaacgga
     1621 aaaagtagtg gcgggaatga gtcggttatt ttaactgttc cagaaggcta tcggacacca
     1681 attagttttg aacaactggt tgggtcaata gaccgttcta ctttgaacag tgctcaacta
     1741 tctattggcg cagacggagc cattaaatgg agaagaaatt caagttatgg atcagcttat
     1801 tcatttgtta tcacttattc aatttaaggg aggaaatcta atgaaagtag tttacaaatc
     1861 aatcaagcct tacggattcg agcaaatcat tttgaacaat caagaaaata tccctgaaaa
     1921 ctgtacagag attaaaccac cagttcctaa ctggagacca agatttgatt ttgataaaaa
     1981 acagtgggtt gagttagcta ctgaagaaga gaaaaaaggg aatgctgttg atagcgtaga
     2041 tgaattggct aatattaagg cattatacga aacgctgaag gcagaaaatg atgaattgaa
     2101 acagctaaat tctaaagcaa tgctaaacaa tgtagcaatc aagcaagaaa atgtcttatt
     2161 gaaagaaaag tcagaaagtt tagcgcagtt gaattcaaaa acgatgcttg cttctgtaca
     2221 aaataccaag gaaatcgaag aaatcaaaaa acaattacaa ggtgggaagt aacatgtatt
     2281 catatgaaga tatcaaacta atgtatgact ggggctgttt cactaacgaa caagtaatgg
     2341 ttttcgttcc attatgcatt acagacgaag aagcagataa aatcattaat aaagataaga
     2401 gcgcatctta attgatgcgt ttttatttaa ggtaaaggag ttgtcacatg attaatttag
     2461 gggaatgggg aatgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa
     2521 ttagaccgat tgctgcatct ttctcgaaaa ttactgagac tctttcaaaa gtaagccaca
     2581 atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg
     2641 aagaactaaa gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca
     2701 cgcaacaaat caaaactttg tttagggaga gaagaaaatg aataataaaa cgttcgaagt
     2761 actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg
     2821 taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc
     2881 gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag
     2941 cgaagaagga ggaaaca
//
LOCUS       DACENJ010000235         2925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.242, whole genome shotgun sequence.
ACCESSION   DACENJ010000235 DACENJ010000000
VERSION     DACENJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.242"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            132..2150
                     /locus_tag="IW748_002438"
     CDS             132..2150
                     /locus_tag="IW748_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ1105334.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2210..2902)
                     /locus_tag="IW748_002439"
     CDS             complement(2210..2902)
                     /locus_tag="IW748_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1105335.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          910 a          554 c          515 g          946 t
ORIGIN      
        1 tcactcaagt tatatgtaat ttccctttag tttcttatcg cgtatctaca tttatgaaac
       61 gtgaatttcc aaactcaggg aaggcatggc tgataaatat cgggcagttt tttatttgct
      121 atttattagc agtgtccctc ctagttatct ttttatttta tatacaaaaa atccgtttca
      181 ttcatcgttt catcgaatac ttcgcttttg attcgattga acaattaact cagcgttgga
      241 aaaaaagaat tcattggctt tttgttcata aaagttattt tttagttgca gcattttttt
      301 actgctttac cttcgtgcag attttcgttt tagaaccaaa agaaggttgg aaagaaacta
      361 tccaggttgc cttaaaaata tttactcagc gacaagcacc catgatcctg acaatcatca
      421 ttattatggg atttttcttc ttactattgt tgatttccaa tcgtttttgg tacgccttga
      481 ctgctacctt gatcattaac ttactgctca ctatttcgac agtaatcaaa atggaaatgc
      541 gggaagagcc tgtctttcct tcagatttaa aaatgctgac cggattgtca gaactattat
      601 cgatggttag cccagtctta ttaatcgtgg gcggattgat ccttctccta ttattgatta
      661 ctagtattat tgtacaacgt agactacaaa aacagtattc cttgaagatt cattggaaac
      721 gtcgatttat cagtatcgct gtattattag gatgtttttc gggtgtcttt ttcattaatc
      781 acaaaaattc gccttctttc ttgctgttta atttatttaa agtaaacaag actttcttta
      841 accagcaagg cgccgtaaaa gataacggac cagtcattca attcctaaac aatatcgaca
      901 ttaaaatcat ggaagaacct gctggctatt cgaaagaaaa aattaatgga ataatgaaaa
      961 agtacaacac aaaggcagat gaaatcaacc aaacaagaag tgactggtta aacaatcaga
     1021 cgatcgttct caatttaagt gaaagcttca gtgatcctgc acgtgtccct aatctgacag
     1081 tacctacaga ccctattcct tacatcaaaa gcattataac agaaggaaca ggaggattga
     1141 tgttatccgt cggttatggt ggcggaacag ctaacatgga atgggaagga ctgacaggct
     1201 tgagtatcag taacctgtct gcttcgttag ttacacctta tacccagcta gtggagcgtc
     1261 agaaaataag tccaaatatt accgatctat tcgatgaaaa aatagccatc catcctttta
     1321 cagctgctct ttatcgcaga aaacaagtgt ttgataaatt tggttttgac aggttctatt
     1381 atttagacag tccggataaa ttaacttata cggataaaat agaacaaagc ccacgcatat
     1441 cggatgattc agcatacaaa gaaaccttga aagctttaca aagcaataca gaaacgtcac
     1501 aattcatcca attatccact atgcaaaacc atatgcctta tgatgattac tatagtgaat
     1561 tgaactacac tgctgaagga actgctgtca ttcctaatag aagaaaagaa ttacaaactt
     1621 atatgcaagg ccttcactat accgatacag cagtaaaaaa attcatccaa gaaatcgaca
     1681 aaatcgataa accgattacc tttgtttttt atggtgacca tttacccgca atctatagtg
     1741 ggaatgattc gaaaaaatat ggattagaac agcaccagac agattatttc atttataaca
     1801 acaagtatag tcgtgaacaa gcagctacgt taaataaaga agttgttgct cctaataact
     1861 ttggtgctat ggctcttgag catgcgaata tcaaagtaac tccttactat gcgttgttga
     1921 cagaagtatc taatcatcta cctgcattaa caattgatcc tacagaaagc ttgtctaatc
     1981 gttttaatgg aaaacaggtc tttgttacag aggatagtca aattatccaa gaaaaaaatt
     2041 taactgagaa acaaaaagaa attctagaag attatcgtct gatacaatat gatcttgttg
     2101 caggtaacca gtacagtgca acatgggcga cacaaaaaat aaaagactaa caaataaact
     2161 gagcaagggg ttgtgataaa agacatgtca caaccccttg tttatctcat taatttgctt
     2221 gtccataaac gatttgatta aagccatttt tcaattcatt attcgctgtc tcgttgaagt
     2281 aaagataatt atcgggattc ccctgatttt gttgtgtcac caatactttt ggctgatttt
     2341 gctgaaaccc gaatagatag acgtgctttt tcagatatgg ttctgtctct gcatcggaat
     2401 agacggctgt gatttgatag tctgcttgac ctgtcccact ttctgaccat tctgcagaaa
     2461 caggcactcc accaatagcc atcttccact ccccgttcaa aatagcttga gggactttaa
     2521 gaccatataa gtccacactc atttgattcg tataactttt gtattgttgt cccattgttt
     2581 ggccccattt ggtcataaat gcctctaatt gtgcggattt gtctgcattc cacagtgttt
     2641 gttcaacttg ttcgctgctt tctgaagagg aagatgaagc ctctacagaa gaacttgaag
     2701 tagatgtttg agttgtttca gtcgttttgg aagactctga cgaagtagag gtatctgctg
     2761 tttctgtact tgctgcagtt gacttgctct ctgtgctagt tgttgtagta gttgcaactg
     2821 ttgctttcgc tgtttctttt gttgattctt ttttcgattc attcccgttg gtacaaccag
     2881 ctaacaaaaa agctagacca attgttacta aaatcctttt tttca
//
LOCUS       DACENJ010000236         2886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.243, whole genome shotgun sequence.
ACCESSION   DACENJ010000236 DACENJ010000000
VERSION     DACENJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.243"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(61..2727)
                     /locus_tag="IW748_002440"
     CDS             complement(61..2727)
                     /locus_tag="IW748_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1105336.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
BASE COUNT          796 a          648 c          525 g          917 t
ORIGIN      
        1 ttgaaattgc accttgtgac tcagaattaa acggcaggct cacaacctcg ttgattcgtt
       61 ttatttattt ccttgtactc gtacaatgat caatttagtg atttccatca aaacaattgt
      121 cacaagtgcg attcccatac atgtaaagac ttctgtcaat ccaaatgctg atgggataga
      181 gaagacttct ttcagtcctg ggatcattgt taagcagtaa agcgcgctac atacaactac
      241 agctagtaag acacttctgt tagagaagaa ccctgcttgg atagctgttt gtgtgtttga
      301 acgtgctggg aatgtttgca atgtccgaca ccaaatcaat gttgaaaatg acatggctac
      361 accgaattca ggtgaatgct gcatgccaag ccattgagca aagataacag caatcccaat
      421 cagcacacca cgatacgtta cagaaatcaa tgttttgtcc gtaaagattc ctgaatttgg
      481 atctcttggc gggcggttca tcgtagcagg ttcagctttt tctgtaccta atgcaattgc
      541 tggcagtgaa tcattgacta aattgatgaa caacagttgt aatgctgtaa atggattgga
      601 ccagtcagca actaaggcaa agatgatcgc aatgatcgca cctaaatttc ctgcaaatag
      661 ataagaaata gctttcttga tattatcaaa cacattacgt cctacttcta ctgcgctgac
      721 aatagaagca aagttatcat ccgtcaatac catcgctgct gcatcttttg ccacatctgt
      781 acccgttccc atagcaattc cgatgtctgc ttgtttcaat gctggtgcgt catttacacc
      841 atcacctgtc atagctgaga tatgaccttt attttgccaa gcacgaacga tacgaatttt
      901 attctcaggt gaaacacgtg cataaacagt gactttttct aaaacttcgt ttagttgatc
      961 ttctgtcaat tcatctagtt ccgtcccggt caaagccagg tttccttctt caaaaatccc
     1021 gatttgtttt gcgatagcaa cagctgttgt tttgtggtca ccagtaatca tgattgtttt
     1081 gattcctgca cttcttgctt ctgttaccgc ctgagtcact tcagcacgtg gaggatcgat
     1141 catagcaagc aaaccaacaa ggatcaaatc gtcttcatct tcaaatgata aatcaaaatt
     1201 atcaagtgga cggtaagcaa aagctagtac acgtaatgcg cgtgttgaaa aagcttcatt
     1261 ttgctcttgg aattttttca tcaattcatc cgtcatcgga acgacttcac catttagcaa
     1321 tactttttta ctgcgttgga agacgatatc tggtccgcct ttaacaaaca agtaattttt
     1381 gttatcaatt gtgtgtccag tagacattaa ttttcgatct gagtcaaaag gaagttctgc
     1441 ttgtcttggg tagttgtttc ggatttcctg ataaggctgg tttaattttt cactaaagtc
     1501 gataaacgct acttccgtag ggtcgcccaa tttagtaccg tcttcactga tcgatgcatc
     1561 attcgctaaa acactgattt cgatcaagcg acgttcgtca gctgtccatt ttgaagggtc
     1621 atttgtgaaa tccccagtat cgccattacc taaaaagtag tcaacaactg tcattttgtt
     1681 ttgtgtcaat gtaccagtct tatctgtaca gatgatactt gtcgaaccaa gtgtttcaac
     1741 tgctggaagc ttccgaataa tggcatgacg ttttgccatt ttattcgttc ccaaagataa
     1801 cacgattgtt acgatagact gtaatgcttc tggaatagca gctacggcta cggctacggc
     1861 aaacatcact gcgctgacga tatctgcagt catatctcct gtaccttctc caagataaat
     1921 acgaaacagt tggataccta aaatcaataa agacaatgcc aaaatagcca aacttagttt
     1981 tttactgaat ttatccagac ctcgttgtaa cggagtgact tgctcagttg ttgattccaa
     2041 aaggccagca acttgtccga tctccgtatt attcccagtt gaagtcacta agaatttacc
     2101 gcgtccgtaa gttacgatcg ttccactgaa taccatattt gtacgatctc cgattggtac
     2161 cgtacttgtg atatccgtta cttctttttc tgcagaagtc gactctcctg tcagcatacc
     2221 ttcatctact tttaatgatc ccgcatctag taaacgtcca tcagcaggta cataatcgcc
     2281 tgcgtctaac aacacgatat ctcctaccac aagttcgttt gccggaatcg ttttttcctt
     2341 accatcacga atcactcgag catcgggcgc tgaaaggttt ttcaacgctt ctaaagaacc
     2401 ttctgctttt tttgtttgga caacactgat tatagaattc aacatcaata ctgcaaaaat
     2461 aactaagaat tcgatgtagt cacccatgac catctgaatc agtgcgacaa tcagcaatac
     2521 gataaccatt gcatctttga aagtttctaa gaacaatttc caagttggct tcttcggctt
     2581 ttcttttagc tggttcaagc catctctttc taatttttcc ttcgcttgcg tggtgctcaa
     2641 tccatcggtt gagctctgcg tttgctgcat caacgagtcg atcgtttctt tatagaccgc
     2701 ttgttgtgct tgttccttgt tttccaaatt tcatccccca ttttttgatc ttggccctag
     2761 tcctgtctga aaaaaaagag acctaagaca aatacgcatt tgtcttaggt ctcaccattt
     2821 catacaatac cagaaaacca tttggttaac ttactgttga tattatcgca acaaacgttg
     2881 ccggtt
//
LOCUS       DACENJ010000237         2881 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.244, whole genome shotgun sequence.
ACCESSION   DACENJ010000237 DACENJ010000000
VERSION     DACENJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.244"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1015)
                     /gene="tsaD"
                     /locus_tag="IW748_002441"
     CDS             complement(<1..1015)
                     /gene="tsaD"
                     /locus_tag="IW748_002441"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1105337.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1012..1476)
                     /gene="rimI"
                     /locus_tag="IW748_002442"
     CDS             complement(1012..1476)
                     /gene="rimI"
                     /locus_tag="IW748_002442"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1105338.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(1483..2025)
                     /gene="rimI"
                     /locus_tag="IW748_002443"
     CDS             complement(1483..2025)
                     /gene="rimI"
                     /locus_tag="IW748_002443"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1105339.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2009..2833)
                     /gene="tsaB"
                     /locus_tag="IW748_002444"
     CDS             complement(2009..2833)
                     /gene="tsaB"
                     /locus_tag="IW748_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1105340.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT          801 a          651 c          485 g          944 t
ORIGIN      
        1 attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt
       61 gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag
      121 ggggaatcac tagttctacc tcagggagtt tagttgccaa agcatcctgc aatccttcac
      181 gcaacccttg attggccgct acacctccag ctataatcag ttgcttgact ggataatttt
      241 gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg
      301 ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa
      361 atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg
      421 gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt
      481 aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg
      541 tctccccaat gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc
      601 cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga
      661 tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct
      721 tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga
      781 ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg
      841 tgatttgttc tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat
      901 gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg
      961 ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat
     1021 cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata
     1081 tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc
     1141 attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag
     1201 taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt
     1261 tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc
     1321 ttcagctaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt
     1381 cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa
     1441 gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag
     1501 ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt
     1561 tgcttgaaac cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc
     1621 taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag
     1681 cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc
     1741 taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag
     1801 atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa
     1861 ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa
     1921 ttctcttagt acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt
     1981 atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag
     2041 gtgtgtgcgt ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa
     2101 aagcttgttt ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac
     2161 ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt
     2221 cagagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta
     2281 gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat
     2341 tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca
     2401 caataatatt atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta
     2461 ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc
     2521 ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt
     2581 gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga
     2641 tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt
     2701 ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct atgacattct
     2761 cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag
     2821 tacaataata catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa
     2881 a
//
LOCUS       DACENJ010000238         2811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.245, whole genome shotgun sequence.
ACCESSION   DACENJ010000238 DACENJ010000000
VERSION     DACENJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.245"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            129..2480
                     /locus_tag="IW748_002445"
     CDS             129..2480
                     /locus_tag="IW748_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1105341.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2560..>2811
                     /locus_tag="IW748_002446"
     CDS             2560..>2811
                     /locus_tag="IW748_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105342.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESP"
BASE COUNT          960 a          357 c          571 g          923 t
ORIGIN      
        1 ttaataaaac agcacaaaga gatttctttg tgctgtttta ttatttttag ttgttgacaa
       61 acactaaaat ataaaatata atcaacacat aaacgaacgt atgttcggca agaaagcagg
      121 ggatttttat ggatgagatg gttttaagta ctcagaaatg gctaaacaag aaatacagta
      181 atgttacagg gtttgataag gttccagaaa atgggcgaac agggtggcct acaatctatg
      241 gacttattga aggactacaa gtagaacttg gtattactaa tctagtagct aattttggac
      301 ctaccactga aaaaatgtac gataatcagg ttactcctaa atggggcaaa aatctaccta
      361 aaaatattgt atttcttatt caaggtgcct tttggtgtaa ggggattaat ccaggtggct
      421 tcgatggggt atatactcca tatttagata ctgctgtaaa agagttgcag actgatgcag
      481 ggtttaatgg aagcgctgta actggagttt tggatgcgaa atgggccaaa gcgttatttg
      541 atatgagtgc ttttgttctt gttcccggcg gagatagtaa aattagaaaa atgcagcaat
      601 ggttaaatgt aaattattta gaatacacgg gaataatgcc ttgtgatggt atttatcaaa
      661 gaaattcaaa tcaagcatta atttttgcat tacaagctga actaggatat tctcccagtg
      721 aagcaacagg gtcttatggt aatgggacaa ctagtaaaac ttatcctgtg tctgaaggta
      781 actcgggaaa ctatgtaaga ataattcaat atggattata tgttaatggt tattttgaag
      841 acggtacatt cgatggtatt ttcactaaat atatgggttt agaagtactg gcattccgaa
      901 aatttatggt attgcctgaa tatactgaaa tagcagatgt tgttgtaata aaaggactat
      961 tgtctagcgc tggagatatt agaagatctg cagatggcgc agatacttct acgcaattaa
     1021 ctagatctca aatccaaaca ttggttgata atgatataaa aatagttggt agatatctta
     1081 ctggtactgt tggaattgga aaagatgaac gaaataagta tctaactaca gaggaattga
     1141 ataatatttt ttctaaaaat ttatctgtgt ttcctatcta tcaagatgga ggtgcagcat
     1201 tagcttattt tacttatgat cgtggcttat cagatggtaa gaaggctata gatgcagcaa
     1261 aaaatctaaa tataccatta acaacagtta tttattttgc tgttgatcta gacatgttag
     1321 gggaagaaat tcttgcatat gctggagaat attttaaggg ggtatctgct gtaatgtcat
     1381 atagcgggta tcaaacgggt gtttacggaa caagaaatgt ttgttctcaa ataatcaata
     1441 acgggtatgc ttcgtttgca tttgtttcag atatgtcgac tggttatagt gggaatttag
     1501 gttttcctat gcctagacaa tggtcttttg atcaatttgt tgaatttacc ataggaagtg
     1561 gcaacggagc tgttggtatc gatttaatcg ctacatcggg tagagatagc ggatttaatg
     1621 agttatcaaa tgatactaat aatgataatt acattgccaa atacaatcag aaggttataa
     1681 atcagatggt acgtgcgtat caatatctat cacaagctgg tcttgataat ccttggaacc
     1741 ctcatttgac attttataga tatgtaaatt attctggttt gtcatgggac atcatttctt
     1801 ctcctgtaac agagcatgat agaaaaattt atgatgaata taggaataaa ttaactaata
     1861 gcgagggcct gtataattat tttatagatc ctaatactgg ttcaataata ggtttgcctc
     1921 acttaattgt gacgttacaa tcacagatgt ttctagccga cacaatcaat gatagtgttt
     1981 ctgactttgc aggttggtta ggagatttaa tgacttgctg gggtgaagtt aagaaattag
     2041 gaatacaaat ggagcaaggt gtatttgcat tagtaggtac ggctgcaggt ggaacattta
     2101 gtctagaaga tttataccaa gatgttgacg cggtaaatgt ttatacctta gcaaaaagaa
     2161 attctgattc aacagcttta tcggctttaa aaacgtacta ttgtgataag ggtgctattg
     2221 atagatttaa tcaatttgtt aagaacagat ttggttcttc agataaaatc gcttcagtaa
     2281 caaatagttt attagaaaat gacgaaacag ttgatatctt aggtgtaggg ataatttcat
     2341 ttttcattga taaaatagtt aataattcac ttgggacaga ttttacttat cttgatttta
     2401 ttggagatga ggggaaagtt gcaaatggat tttctgaaaa aattattcaa tttcaagaaa
     2461 atagaagtaa acttaattaa gaatcgttta ctatttaaac agtgtccttt tcctttttaa
     2521 atatatacga taaaataagt atatgaattg taaaaggaca tgatgattat ggaagaaata
     2581 gaatggaata atctgactca aaataataaa aataattctg gatgtggaat tatctttatg
     2641 tttgttatag ttttgggaat agtagtactt ttaataactg tcaagatagt agattacttt
     2701 gcgaatactc ctgaagaaca aatacagaga aaacttcctg agtttgaaag agtaccacct
     2761 tatgatccaa gagataattc atttcaagta aaaaaattaa aagagtctcc a
//
LOCUS       DACENJ010000239         2784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.246, whole genome shotgun sequence.
ACCESSION   DACENJ010000239 DACENJ010000000
VERSION     DACENJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.246"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(15..962)
                     /locus_tag="IW748_002447"
     CDS             complement(15..962)
                     /locus_tag="IW748_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1105343.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="IW748_002448"
     CDS             complement(1140..1943)
                     /locus_tag="IW748_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1105344.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..>2784
                     /locus_tag="IW748_002449"
     CDS             2089..>2784
                     /locus_tag="IW748_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1105345.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDV"
BASE COUNT          897 a          452 c          544 g          891 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgtt
//
LOCUS       DACENJ010000240         2774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.247, whole genome shotgun sequence.
ACCESSION   DACENJ010000240 DACENJ010000000
VERSION     DACENJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.247"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IW748_002450"
     CDS             complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IW748_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1105346.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTE"
     gene            complement(734..1573)
                     /locus_tag="IW748_002451"
     CDS             complement(734..1573)
                     /locus_tag="IW748_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1105347.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1758..2645
                     /locus_tag="IW748_002452"
     CDS             1758..2645
                     /locus_tag="IW748_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1105348.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT          997 a          548 c          418 g          811 t
ORIGIN      
        1 tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
       61 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
      121 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
      181 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
      241 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
      301 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
      361 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
      421 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
      481 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
      541 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
      601 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
      661 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
      721 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
      781 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
      841 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
      901 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc
      961 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     1021 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     1081 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     1141 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     1201 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     1261 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     1321 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     1381 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     1441 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     1501 tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca atcaaccgaa
     1561 taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg
     1621 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
     1681 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
     1741 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
     1801 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
     1861 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
     1921 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
     1981 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
     2041 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
     2101 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
     2161 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
     2221 ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggggaaaat ttctccaagc
     2281 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
     2341 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
     2401 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
     2461 tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc
     2521 tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaacaatcc
     2581 gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa aatatattca
     2641 aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat ttttggaaat
     2701 tccttcttat ttttgaggca aaccactttt gccacaactt catcggggcc tttttttatt
     2761 tgtaccaaaa caaa
//
LOCUS       DACENJ010000241         2735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.248, whole genome shotgun sequence.
ACCESSION   DACENJ010000241 DACENJ010000000
VERSION     DACENJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.248"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            414..1031
                     /locus_tag="IW748_002453"
     CDS             414..1031
                     /locus_tag="IW748_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1105349.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1072..1371
                     /locus_tag="IW748_002454"
     CDS             1072..1371
                     /locus_tag="IW748_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105350.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1405..1572
                     /locus_tag="IW748_002455"
     CDS             1405..1572
                     /locus_tag="IW748_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1105351.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1617..2180)
                     /locus_tag="IW748_002456"
     CDS             complement(1617..2180)
                     /locus_tag="IW748_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1105352.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(2208..2735)
                     /locus_tag="IW748_002457"
                     /pseudo
     CDS             complement(2208..2735)
                     /locus_tag="IW748_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          881 a          458 c          521 g          875 t
ORIGIN      
        1 aagaatacaa ttatattgta aaggcaaaag tatcaaaaag atttattttt aaccctaaat
       61 tagaaatata tagtgttgta atagaataat agtgttaaaa aaattgtaaa agttaaagaa
      121 gtcaaattct gatgtgaaaa ataaaatata ccatggaaga cataaaagaa aaactggata
      181 actagtttat ttaatggcag accgttaagg cctgccattt ttatgtattt tagcggaata
      241 aatataatca tgcgggagtg taaacttact gtttcaaaaa gtatactttt tgacatgcct
      301 tgattttctt tgtttatgta tggcataata accacataat acaatttgaa acgtttctga
      361 tttaatctga ttgttttgaa acaattttag tgaaaaaagg gtagataaaa acgatgagaa
      421 aattttttta tatccgggtc agtagttatg atcaatcgat cgaacgacaa ctgatttccg
      481 cgaaggaatt aggaatccct gaagaatata tatttgtaga aaaagccagc ggcaaagatt
      541 ttaaacgccc tgaatatcaa ttaatgaaac ggatgtttcg aaaaggagat ttactctatc
      601 tacaatcatt agatagacta ggcagaaata aacaaatgat tttagaagaa tggcgagaat
      661 taactaagga aaagcagatt gatatttctg tgttagacat gcctctgtta gatactgcta
      721 gttacaagca actagatggg ttagagacgc ttatctctga cttaattctg caattactca
      781 gctatatggc cgaagacgag cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag
      841 ctgcgaaaca aaagggtgta aaattcggca gaaagaaaat tgctttacct cctgaatttc
      901 ctgcgatcta ttctgactgg aaagcaggaa agataactgc agtctatgct atggatcaat
      961 tgggcatgaa acgaaatacc ttttatcgac gggtgaaaga atatgaacat accttgcacc
     1021 acttttcttg atagatataa cagctattag acgggaaggg agagtttgaa aatggactta
     1081 acgaatcaaa gggtcgtttt tactggaacg ctgcagcaaa tgacacgaac ccaagccatc
     1141 ggtttggttc actctttaaa cggccattgt caatcaagtg ttacttcaaa aacaaatttc
     1201 ctggtttgtg gtcagtattc taagtcgttg tttgatgatt ctctctatac aaaaaaggaa
     1261 caaaccgcgc gggacttgat tgcgttaggt catgagatta ccattttatc agaagtcgaa
     1321 ttttatgcgt taattagtca acagttgaaa gaatggcaac ggtatttata atcgttagta
     1381 aaatttacaa ggaggaaaat ttttatgaag cgaaaatctt atgatcgttg gggcattgaa
     1441 ataactagtt ttaatcgaaa tattgctttt cacgtggagt gtgactgtgg ggaattagca
     1501 aaaagtgttt atcaaaaaaa ttacttttac tgtcctaaat gccatcgccg atacggggta
     1561 catggaacat agaaacgaaa tagtgcggta gaaaagcgaa tgaatgtagt atatttctac
     1621 aaatcattcg ctttctttat tttataaata gttggtcgag agatcccgtt ttcttttgca
     1681 atctctgcaa ctggaatgcc ttgctttaac atgtccacca cggttttaaa aactaagcgt
     1741 ttttgcggat ctgttgagtt aatagaatac tcgattggtc gtcccttata tcggccggct
     1801 ttttgagcca ataatactcc ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt
     1861 gcaatataac ttagcagtga taaaaacata tcgaacatag cggtatccag gagttcattt
     1921 ccagtattaa aatctaaaaa ctgtgcatct aatattttta acgctacttt tttctgtttc
     1981 ataaaagcaa ctgtattttt tatatcttca taatctcttc ctaaacgatc tagtgatgta
     2041 acgatgacgc aatcaccttc acggatataa tttagtaatt tttgaaactc aggacgctca
     2101 gtatctttac cactggcttt ttcttggaat aatttatcaa tgttaagtcc ttcggttctg
     2161 ttgcgaagtt tgaaaatcaa acgcgccctc tctgaactcc aaatatctta ggctgttatt
     2221 cccattaata ccttgatttc agtagacacc gaaaagccga agagcgttcc atttcttcgg
     2281 ttctttttat atattcctcg aatggtctcc atgcccttaa tcgtggaaga ggctgtacgg
     2341 agactttgat aaaatttatt tcgtcgttta ataggtcgat ggtcttgttc tattaaattg
     2401 ttaagatact tcacagttcg gtgctctgtc ttagtatata aacccacact ctgtaacttt
     2461 ctaaaggcgg agccaagaga aggtgcttta tcggtcacaa ttgctttcgg ctcaccaaac
     2521 tatttatgga gtcgttttaa gaaagcatag gctgcttgcg tatcccgttt ctttcgtaac
     2581 cagatatcta aggttaagcc gtccgcttca attgcacgat aaagataatg ccaacgtccc
     2641 ttaattttga tataggtttc gtccattttc catgaataga aggattgtct atttttcttc
     2701 ttccaaagat aatagaggac tttgctgtac tcttg
//
LOCUS       DACENJ010000242         2648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.250, whole genome shotgun sequence.
ACCESSION   DACENJ010000242 DACENJ010000000
VERSION     DACENJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.250"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            240..656
                     /locus_tag="IW748_002458"
     CDS             240..656
                     /locus_tag="IW748_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1105353.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            670..1236
                     /locus_tag="IW748_002459"
     CDS             670..1236
                     /locus_tag="IW748_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1105354.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1259..2245
                     /locus_tag="IW748_002460"
     CDS             1259..2245
                     /locus_tag="IW748_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1105355.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2400..>2648)
                     /locus_tag="IW748_002461"
     CDS             complement(2400..>2648)
                     /locus_tag="IW748_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1105356.1"
                     /translation="STDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSK
                     RALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          810 a          495 c          544 g          799 t
ORIGIN      
        1 ttgaaaagta attttcgtaa attccttctt atttcttgaa gttgcccact tctgtcacaa
       61 cctcttgtta taaacggtgt tccacctgct gcttctcggt aataagccca aagaattaga
      121 aaacatggaa agctgttttc tattcttcgt tcttattact caaagcaaga cacaggtgtc
      181 acaacctctt gtttttatgc taaaattggg tgagttaatc tggaaaaagg agaacaaaaa
      241 tgagccttaa agaatttgct gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt
      301 tgcttttgac tgttctttct tttttaccag tcatgatttt tggtcttcag caaaacaccg
      361 caacagcaga aaaagaagcg gtactgcgtg tggatggcac agaaatcaaa acattccctt
      421 tgatggctgg agaagagagc aacacatata cttacaaaga tgaacacgga gattataact
      481 tgatcgagat agatggcgat cgagtacgga taaaagaagc agattgcgat gaccagatct
      541 gcgtacgtcg aggctgggca acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat
      601 tagtgatcga agtccgttca acagatggag gtgacgaaga cagtctgatt tattagacct
      661 tcacacacca tgagtaaatt acaaaaaatg atttatattt cgctacttgt tgcacaagga
      721 gtgatcattg gtttattgga aaatatgatc ccttatcctt tttcttttgc gcctggtgca
      781 aaactaggct tagccaatct aatcacgatc gttgccattt ttaccatgaa aaaacaagac
      841 agcttcttac tactttggct acgactattc ctgactactt tgctaggcgg aacgatttcc
      901 acattccttt atagtatgag tggtgctttg cttagttatt ttggaatgtt attggtcaag
      961 caattaggac caaaacgtgt cagtatcatc ggaattagtg cggccggcgg tttcatgcat
     1021 aatgtcggac agcttttgat ggcttctttc attgcacaat catggacagt catgttgtac
     1081 ttgccagtcc tttcagtttt agggatttta tcagggattg caatcgggat cgcagcgaat
     1141 tatcttttac gacatgtgca gacacttcaa cgattccaaa aagattatga gcagggaacc
     1201 aaacaaaaat ggagcccaat gtttaccaaa aaataatcgt cacacaagga gtttttttat
     1261 gaagctacat ccaatgtggg aaaactatcc tgctttaagt aaagaattga cagaaacact
     1321 aaaacggatg gaagaaactg tccgcctgaa aaacaaacct gtcgaacaag ccatcaaaga
     1381 aacgatccat gccggcggaa agctgttgcg tcctgcctat cagctattgt tctcacaatt
     1441 tggaccagag caagatcgag acaaagccat cgctttggca gcttctatcg aaatgctcca
     1501 tacggccaca ctgatccatg atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc
     1561 taatatccgt agtcagtttg gtaatgtcgt ttctgtctat gcaggagact atttatttgt
     1621 ctgctgtttt aaactattgt ctgattactc cacttccttg aaaagtctcc aattgaattc
     1681 tcgcagtatg gaaaaagtat tgaatggcga attagggcag atggatgatc gctataatta
     1741 tgaactttct gtaaaagaat atttggaaaa tatttcaggt aagacagctg aactgttcca
     1801 attaagttgt tctgttggcg cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc
     1861 aggagatatc ggactttcta tcgggatggc ctttcagatt atcgatgata ttttagatta
     1921 tacaaaagag agccaggaaa tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta
     1981 ttctttaccg ttgatctact ctttgcaaaa aaacaaacca aagctactac cttatttaga
     2041 gaagaaagcg gcaatgacag aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac
     2101 aggaggcgtg gaagaagcta ggaagttagc ggcctcttac acagaaaagg ctttgaagga
     2161 aataaagaaa ttacctgcca cctctcttcg aacaaaagag aatctctttt cccttaccca
     2221 actgattttg gatcgcaaag actgaatagg gaaaggttcg atacgtaaat aaatttctca
     2281 gaaggaaatc tacgtgtttt ttgacaaatt gaattgtagt tcttgtattt atgataaaaa
     2341 gcaaaaataa agagcaacgt ccgttagcga cgttgctctt ttttatcttg aatatttttt
     2401 taccttttcg atttcttata actcataaaa gtgattctta aaccgattac aagtaatata
     2461 acgcctgcga gtgttatcca attattcgat tgacttccag ttttaggaag tgctcttttt
     2521 gatttgtttc cattgtcatt gcttgtttta ttagttaagt taaagttcgt cctgttatcc
     2581 gaacttgttt ttgagaagga gacacttgca tgacgtgtat cgcttgtggt agacgacgtg
     2641 tctgtact
//
LOCUS       DACENJ010000243         2612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.251, whole genome shotgun sequence.
ACCESSION   DACENJ010000243 DACENJ010000000
VERSION     DACENJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.251"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            42..1049
                     /locus_tag="IW748_002462"
     CDS             42..1049
                     /locus_tag="IW748_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1105357.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1061..2065
                     /gene="neuB"
                     /locus_tag="IW748_002463"
     CDS             1061..2065
                     /gene="neuB"
                     /locus_tag="IW748_002463"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ1105358.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2062..>2612
                     /gene="neuC"
                     /locus_tag="IW748_002464"
     CDS             2062..>2612
                     /gene="neuC"
                     /locus_tag="IW748_002464"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ1105359.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNR"
BASE COUNT          957 a          335 c          521 g          799 t
ORIGIN      
        1 atcgaattat agatgactat aattcatagg aggagatata agtgtttaaa ggaaaaacaa
       61 tattagtaac tggcggaact ggttccattg gaagtgaaat tgttcgtcag cttttaaaat
      121 atgaaccaaa agctatcaga atttatagta gaggtgaaga aaaacaattt tatatgcaac
      181 aagagctctc aaatcacact aatttgagat ttttaattgg tgatatcaga gactctcaaa
      241 ggattgatta tgcatgtcgt gatgtggata ttatttttca tgctgccgca atgaaacacg
      301 ttccagcaag tgaatataat cctatggaag ctgtaaaaac aaatgtgatg ggaactcaaa
      361 atgtaataga tgcagcgatc aaaaataatg taaaacattt tgtaggtatc agtacagata
      421 aagtagtgag tcctactaac accatgggtg cgacaaagtt attgagtgaa aaattaataa
      481 tagctgccaa tcagtataaa ggaaaaacag aaacaatatt ttcttgtgta aggtttggaa
      541 atgtcatggg gtcaagtgga tctgttattc ctctttttag taaacaaata aaaaacaata
      601 aagatattac tttgacagat gttgatatga cacgatttat gatgtcaata ccggatgctg
      661 caagattagt tctgtctgct gctatgaatt caaaatttgg agaaacatat gtcttaaaaa
      721 tgccggcaat caatatttta actttagctc aatcattaat aaaaatatat aatgatacac
      781 atgatgaaaa atatagaggt gaaataaaag ttgtaggtat caggccagga gaaaaattat
      841 atgaagagct aatgactaca gacgaaattg aacgctcata tgaaaatgat aaaatgtata
      901 tcattccacc acactatcat gctactgacg aactgtacaa agattatgaa aaaattaaaa
      961 tcactgcata ctcttcagat gcaacagaat taatgagtgg acaggaattt attagaatcg
     1021 caaaagagaa taatgtcgtg acttggtaag gagttttatg atgaaatcag aagtatatgt
     1081 aattgcagaa attggtgtaa accataatgg aagtctagat ttagctttag aaagtattga
     1141 taaagctaaa gaatgtggag ctaacgcggt taaatttcaa acatttaaaa cgaataagct
     1201 agtttccaaa aaggctccaa tggcaaaata tcagaaggat aatcttaaag aggacagttc
     1261 tcaatttgaa atgctaaaaa aattagaatt atctcaatct gattttatta gaataaaaga
     1321 ttattgtata caaaataaaa ttgatttttt gtctactcct tttgatgaag aaagtgcaga
     1381 ctttctaaaa caaattggtg tacaaggatt taaaattggc tcaggagatt taacaaattt
     1441 accttttctt agaaaaatag atatgtatgg gttgccagtt ttattatcta caggtatggc
     1501 tgatattcat gaggtcagtg aggctgtaga ttgtttcata gatagtccag ttactatttt
     1561 acattgtact tcaaattatc cggctagttc tgaagatatt aatttattgg cgattaacac
     1621 attaaaagaa atgttcggga tacccgttgg gtactccgat cattcattgg gatatgatgt
     1681 tggtatttgt gctgttgcga tgggtgctaa ggtaattgag aaacatttta ccttggataa
     1741 ggaactacct ggtccagatc ataaagcttc tttagatcct caggaatttg cagattttgt
     1801 aaagcatatt agaaacactg aaatattttt aggtgatggt gttaagcgag caatgccttc
     1861 tgaacttagt acaaaagaag tatcaagaaa aagcctagtg gtcactgaga atttaaatgt
     1921 gggagatatt cttacagaga aatctcttac aataaaacgt cctggaaatg gaatagctcc
     1981 taagttttta tatcaatata ttggcaaaaa agttaaaaga ccaataagtg cagatagtgt
     2041 tctgcttgag gaagatgttg aatgaaaaaa atacttatag taagtggaac acgagctgat
     2101 tatggaatat ataaacctgt tgctgagaat ttactacaag ataattggga tgtatcattt
     2161 gttgtttctg gaatgcattt atcccataaa tatggattta ctaaagaatt gatcttagct
     2221 gacggattta gaattagtgg agaagttcaa agtttgtttt tagagaacaa taagggaaac
     2281 atggctcggt ctgtggcatt agagataaat ggtttcacaa atatatttga acaaactagc
     2341 cctgattttg tattattact aggggataga ggagaaatgt tggcagctgc gatagcttgt
     2401 ttgtatcttg gaatcaaaac agcacatatt cacggaggcg aagtgtctgg tactgtagat
     2461 gaatcaataa gacatgccat aacgaaaata gcatctgttc attttgctgc tacggaaaaa
     2521 agcaaagagc gtttaataaa aatgggggaa gattcttgga gagtttttcc cgtcggagct
     2581 cctagaatag atactatttt aaataataga tt
//
LOCUS       DACENJ010000244         2589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.252, whole genome shotgun sequence.
ACCESSION   DACENJ010000244 DACENJ010000000
VERSION     DACENJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.252"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..321
                     /locus_tag="IW748_002465"
     CDS             <1..321
                     /locus_tag="IW748_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1105360.1"
                     /translation="VASVKNNLKDKDLSTLLVLGKCFSDSSRIKIFYALETYKEMCVC
                     DLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEEILSAVQAFLKLSENS
                     SMKA"
     gene            338..949
                     /locus_tag="IW748_002466"
     CDS             338..949
                     /locus_tag="IW748_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAQ1105361.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            977..>2589
                     /locus_tag="IW748_002467"
     CDS             977..>2589
                     /locus_tag="IW748_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1105362.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPGVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLT"
BASE COUNT          814 a          410 c          548 g          817 t
ORIGIN      
        1 gtagctagtg ttaaaaataa tttaaaagat aaagaccttt caactttact tgtattagga
       61 aaatgtttta gcgattcttc taggattaaa attttctatg ctttagaaac gtataaggag
      121 atgtgcgttt gtgatttagc agcgatactt aatgctagta ttgctacaac gtcacaccat
      181 ttacgttttt tgaaaaagaa cggaatggcg aaatcacgtc aatatggaaa ggtagtttat
      241 tactctttag cgaatgaaga gattctttcc gctgtgcagg cttttctaaa gttgtcagaa
      301 aatagttcta tgaaagctta gtttggaggt agttattttg ttacaaaaca ttttatcagc
      361 actcgctgta tatatttcta ccagtattga ttatttattt atcttgctga ttattttttc
      421 tcaaaaccat actaaaaaag gtctccgtca gatttttttt ggtcaatatc ttggaacagg
      481 aatcttggta gccataagtt tgtttgcagc atatgtgcta aattttattc ctcaagattg
      541 gatcattggt cttctgggac taattccgat ttatttaggg attcgtgttg cttttgtggg
      601 cgaagaggag gaagaagaag gggaagtcgt tgagaagcta ggatctagag gaacgaatcg
      661 tttgttttgg acagtttcct taataacgat tgcttctggt ggcgataatt tagggattta
      721 tattcctttc tttacctcgt tatctttttc agaaattgtc acttctttaa tcgtctttgc
      781 tatctctgtt gcggttcttt gctatattag ttataaattt gcgaaaattt cttttgtctc
      841 tgaaacacta gaaaaatatg aacgaatcat tgtacctatc gtatttattg gattagggat
      901 ttttattatg atagaaaatg gaaccatcca aactatatta ggtttgtaga gatcttatta
      961 gtaaaggaag caaacgatga gtgaaaataa aaagtaccat gaatggatat tagaaggaat
     1021 cgactgtgca aattgtgcag acaaagtaga aagaggcgtc gccaaagttg caggtgttgc
     1081 caatagtagt gtgaactata tgacagaaac cttgagtttc gaagtgacag aggaaaaaga
     1141 atatcttgtt ttatccaatg tgcaacaaaa agtaaaaaaa ttggagcctg atatcacttt
     1201 aaaaaataaa gtggacggta ggctgattga aataaatccg gcattaatca agaaaagtaa
     1261 tttaaaagac gataaaaaaa actacgaatg gatattagaa ggaatcgatt gtgcaaactg
     1321 tgctgccaaa gtggaaagag gagttgctac agttcccggc gttacaaaca gcagcgtcaa
     1381 ttttatgaca gagacactga gcttcgaggt agcaggtgaa aataaaactg acgtgttggc
     1441 tagtgtaaaa caaacagtag gaactttaga acctgacgtt gttctgcgaa aaaaatcgga
     1501 cggtacattg attgaacatg gaactgactc taataaaaat tcgacttcta acaaatcgat
     1561 ttctgataaa gatgagacaa accaaaagca gacgaataga gcttatctgg ccagccttct
     1621 cggaaataaa actaaaatta cccttattcg tttaatacta ggattaggaa tattagcgac
     1681 aggaatattt ctccctatgg atggacaagc ctctcttggg ttgttcattc tggcttattt
     1741 aatcaccggt tatgacgttg taggacgtgc cattagaaat attttccgag gtcaagtatt
     1801 cgacgagaac tttctaatga ccattgcgac gttaagtgcc ttctatattc aagagtatcc
     1861 cgaagctgta gcagtcatgc tattttatca agtgggagaa gtattccaag atattgctat
     1921 ttcaaaatct cggcgctcca ttgcggacct aatggatatt cgacctgatt atgccaattt
     1981 agtcacagga aacaacgtag aaaaagttgc tcctgaagca attaagattg gaaatattat
     2041 tctcattcgt ccgggcgaaa aagtgccatt ggatggaaaa gtagtggaag gtgcttctgc
     2101 gatggacact tctgctttaa caggcgaatc cgttccaaga agcgtgaagc cggaagacac
     2161 tattttgagc ggatttatta ataaaaacgg attgctaaaa gtagttgtag aaaagccctt
     2221 ttcggagtct actgtgaaga aaattttaga tttggtccaa aatgctagtg gccggaaagc
     2281 acctactgaa caattcatta ccaaattttc tcgctactat actccaattg tggttatagc
     2341 tgctgcctta ctggcaattg ttccgccatt gctagtacca ggcgctactt ttaatgaatg
     2401 gatatatagg gccagtatct ttttagttat ttcatgccct tgtgctttag tcgtttcgat
     2461 tccagttggt ttctttggtg ggattggttc tgcttcccgc aaggggattt tagtaaaaga
     2521 aagcaatttc ctcgagggtt tgaacgatct taaatacgtg gtaatggaca aaacgggtac
     2581 attgacaaa
//
LOCUS       DACENJ010000245         2566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.253, whole genome shotgun sequence.
ACCESSION   DACENJ010000245 DACENJ010000000
VERSION     DACENJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.253"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            433..660
                     /locus_tag="IW748_002468"
     CDS             433..660
                     /locus_tag="IW748_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1105363.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            783..1109
                     /locus_tag="IW748_002469"
     CDS             783..1109
                     /locus_tag="IW748_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1105364.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1177..1890
                     /locus_tag="IW748_002470"
     CDS             1177..1890
                     /locus_tag="IW748_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1105365.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2200..>2566
                     /locus_tag="IW748_002471"
     CDS             2200..>2566
                     /locus_tag="IW748_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1105366.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFET"
BASE COUNT          966 a          391 c          534 g          675 t
ORIGIN      
        1 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
       61 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
      121 aaaggataag tttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
      181 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
      241 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
      301 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
      361 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagca aatccctacg
      421 atcacaaaaa aaatgctgac ccagcagtta agagagttag aacaagatga aagtatccat
      481 cgaaaagcct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
      541 tcattaagag agattctagt agcgttgtct gtttgggaag aagaacgtat cgaaaaacaa
      601 aaagcctcaa gggaagaaga cacgctactg catacacatg gtggctacct aaattattga
      661 ttttctatga catttatttt aaataaaaga aaacaggagt ttttttaaaa gcatcatcta
      721 ttttttatgt aaaaaaatgg tatgattaat caagtaagaa gtgattggaa gaaggcaagc
      781 aaatgaatag tatggaaaaa gtcaaccagt ttagagatga acgtaattgg cgacaatttc
      841 ataacgaaaa agatttggct atctctattt ctttggaagc atctgaattg ttagaacttt
      901 ttcaatggaa aacgccggaa gaagtaaaag aaacacaatt agaacgaatc aaagaagaac
      961 tggcagatgt attgatctat tcatacatga tggcagacaa ccttcacctg gatctagatg
     1021 agatcattga agaaaaattg atcaaaaata atttgaaata tcctgtagaa aaaagcgcag
     1081 gtgtacgaaa aaaatacaca gagttgtaga aatgtgtcat tcggctgttg gaaagagcaa
     1141 aagaaaaaca gtaaaatgag tctggaggga gagaaaatga aaaaaaaagg aaaagcaaaa
     1201 agatggctag tcaatatcct cttgttcctc ttgttattag taggactagc tttgattttc
     1261 aatgaacaga tcaaggatta ttttgtcaga gagacaggag ataaatatgc tatagcgaat
     1321 gtaacaaaag aagatctgaa aaagaataac gataaagatg tcagttttga ctttgatgcg
     1381 gtagaaccaa tgacgacaga aggggtcatg aggtcgcaaa tgagaggaac agatctacca
     1441 gtgattgcta gtatcgcagt cccttcagtt tcaatcaatt taccggtttt caaaggcttg
     1501 gataatacgt cactacttta cggagcagga acattatctc cagatcaaga aatgggaaaa
     1561 ggaaattatg ccttagcaag ccatcgtgca acgaaccctg aattgctgtt tacaccgcta
     1621 gaaaatttag agatgggtgc caagatctat ttgacagatt tagaaaatgt gtatacatac
     1681 aaaacatttt ttaaagaaaa agttgctcct acagataccc aattactgaa tgaagtcgag
     1741 ggaaaagaaa ttgtcacact aattacttgt ggcgacatgg acgccgtcac gcgactagtt
     1801 gtacaaggag aactggaaag tgttacatcg ataaaagatg cgacagatga tatgagatct
     1861 gctttcaatc tagagacaaa aacattttag gatactggga cataagtcgt tacagattaa
     1921 aaacagccaa aggatatgaa tcgataagga aaaaccaatt tgctgaaaaa aagagaaacg
     1981 caaagaggtg catctctgat ttggttgttt tctagataga cttgtgttcc agttttttta
     2041 ttttttatat gaaaaggctt gcataaataa aaattcgatg gtatgatgat tttgaataag
     2101 aaatggattg ttactgtacg gcaggcaaaa ccagaattca gctaagaaac agtaggcgaa
     2161 ttctaggttt ttttattttt cttgagaggg cgaagcaaaa tgaaaattga taaaattttg
     2221 aataacaatg tcgtcatttc gaaaaatgga ttcggggaag aagtcgtttg tatgggaaaa
     2281 ggactagctt ttcaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     2341 tttgttttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     2401 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     2461 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     2521 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaa
//
LOCUS       DACENJ010000246         2565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.254, whole genome shotgun sequence.
ACCESSION   DACENJ010000246 DACENJ010000000
VERSION     DACENJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.254"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..1615)
                     /locus_tag="IW748_002472"
     CDS             complement(83..1615)
                     /locus_tag="IW748_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1105367.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            1849..2550
                     /gene="nagB"
                     /locus_tag="IW748_002473"
     CDS             1849..2550
                     /gene="nagB"
                     /locus_tag="IW748_002473"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1105368.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
BASE COUNT          772 a          460 c          506 g          827 t
ORIGIN      
        1 attggccacc atttattcag aagaaggagt tgtgacaaaa cttttgtcac agctcctttt
       61 tttcaactat tcaatcaaac gtttattttg gttcaatctc aatgactcgt tgcacaagat
      121 cttgataaaa ttcttgttca tgagagacga tgattactgt tcctggaaat ttctgtatcc
      181 cgtcttgtaa gctttctttc gtttcttgat cgatatggtt cgtcggttca tcgagaatca
      241 gtaaattacc ttggttcatt gtcaactgtg caagtttcac cttagtttgt tccccaccac
      301 ttaatgtagt gagaggttca cttgctaatt gattaactaa ccctgcttta gctagttggc
      361 gacgtaattc tttgatagtc gcttttggaa attgactgct taaatattgt agtggtgttt
      421 ctaatggatt ttcccagtac agatcttgtg aaaagtaatt gatttttgtg ttatctggga
      481 agtggaaatc gccacttatt ttttcaatct caccgatcag tgtttttaat aaagtagatt
      541 ttccaatccc gttgaagcct cgaatggcaa tattttcacc aaagcggatg gtcaaattga
      601 tgggtgcaag tagcggtcga tcatatccga tgactaaatc agttgtttcc actgctaatg
      661 tcgtgacaat aggcgagaat ggaaaatcaa agacagcttt acttgctgaa gctggaggcg
      721 tcagccgttc gattttatct aattgttttt gtcggctttt tgccatcgtt gatcgggaac
      781 cggctttgta ttttcgaata taagcttcgg tcttttcgat ttgtttttgt tgagcatgat
      841 attggcgcaa atagctttcg cgattttgtt ccttttgttt caatgcttgt tttagatggc
      901 cagtatactt agtaatcttt ccaaactcga tgtctgcgat atgggtggtg atctgatcaa
      961 gaaattcttg gtcatgggaa atcacgatat acgcaccgtc aaaatcctga agaaactgcg
     1021 tcagccattg gatatgctga tcatctaagt gattggttgg ttcatctaag atcagaacat
     1081 ctgggttttc taaaagtagt tttgctaaaa tcacctttga acgttgtcca ccacttagat
     1141 ttttcaacag tgaatctaaa cctaagacat cgatccccaa cccgctgctc atctcagcaa
     1201 ctaaggtgtc gatttgataa aacactcctt ggtctaattc cgtttggagt ttcccagctt
     1261 tttccagcaa ggaatcatcg aaggtctcgc tatatgtttg gtaaagaaaa gcaatttttt
     1321 cttccttttc gtattctttt gcgtacgcag tttttagaaa ttcttcgatt gttaatgttt
     1381 catcgacttc cacatattga tcgaggtaac ctttgtgaat atttttttgc caatggatct
     1441 gtccgtcatc tggtaagatc tcaccagtga tgattttaat caatgtactt ttaccggctc
     1501 cgttttgtcc aatcagccca agatgttcgc cagcatttaa ttgcagatcg acaccttcat
     1561 agaggatttt gtcgccaaat tgctgggtaa tgttgtttaa tgtcagtata ctcataataa
     1621 ctcctttttc ataaaaaaac tgcagacata aagcgtccac agtgaatcac ttgcttggtt
     1681 ttcatgcaac atggattgtt tgattgaacc atcttatgaa acctaacacg gattcctatt
     1741 tattgtcaag aaaatgaaaa aaatgcaatt tggtctatac taattattgt aaaaagaaaa
     1801 aaagaatgct ataattgggc tataccaaaa tataaaggag caatcactat ggaaatcatt
     1861 cgtgtaaaaa acgctgaaga aggcggaaaa aaagcatttg aaatcatcaa agaaggtatg
     1921 aacaaaggtg caaaagtatt aggattggca acaggaagca caccagttac tttatataaa
     1981 gaaatgactt caagcgattt ggatttcagt gaaatgactt ctgtaaactt agatgagtac
     2041 gttggactag gcggttcaga tgaacaaagc taccgtcatt tcatggatgt ccaattattt
     2101 gataaaaaac cattcaaaga aacgtttgta ccagatggaa aagcagaaga tctggaagca
     2161 gcatgcaaaa agtatgatca aatcatcgat gcacatccaa tcgatatcca aatcttagga
     2221 atcgggcaaa atggacatat cggtttcaat gaacctggtg cttcatttga cggaacgact
     2281 tcagtagttg acttaacaga atcaacaatc aatgcaaaca aacgattctt cgataaagca
     2341 gaagatgtac caacaaaagc tgtttcaatg ggaatcggtt caattatgaa aagtaaagaa
     2401 atcgtcttga tggcatttgg cgaaagcaaa gctgatgcaa tcaaagggat gattgaagga
     2461 cctgtaacga atcatttgcc agcaagtgta ttgcaaaatc acgacaatgt tgttgtgatc
     2521 atcgatgaag aagcagcaag taagttgtaa ttttcaatag taggc
//
LOCUS       DACENJ010000247         2563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.255, whole genome shotgun sequence.
ACCESSION   DACENJ010000247 DACENJ010000000
VERSION     DACENJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.255"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..400
                     /locus_tag="IW748_002474"
     CDS             <1..400
                     /locus_tag="IW748_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL family lipase"
                     /protein_id="HAQ1105369.1"
                     /translation="QAIFGTEKSAQQFESVYRKLLNELQTVGICNIILMEPFVLPITD
                     DRHGWRKDLDPKIQIVRKLAKEYGALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGG
                     HEIIAQSWIDAIAAVDFFSGDKNERKKSEK"
     gene            397..738
                     /locus_tag="IW748_002475"
     CDS             397..738
                     /locus_tag="IW748_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105370.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            768..1127
                     /locus_tag="IW748_002476"
     CDS             768..1127
                     /locus_tag="IW748_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1105371.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            1330..1509
                     /locus_tag="IW748_002477"
                     /pseudo
     CDS             1330..1509
                     /locus_tag="IW748_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            1651..>2563
                     /locus_tag="IW748_002478"
     CDS             1651..>2563
                     /locus_tag="IW748_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1105372.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAE"
BASE COUNT          870 a          407 c          558 g          728 t
ORIGIN      
        1 tcaggctata ttcggaactg agaaatctgc tcagcaattt gaatccgttt atcgaaaatt
       61 actaaatgaa cttcaaacag tgggaatctg caacatcatc ttgatggagc cgtttgtact
      121 tcctataacc gacgaccgtc atggctggcg taaagactta gaccctaaaa tccaaattgt
      181 tcgtaaactt gctaaggaat atggcgcatt gctggttcct ttagatggta tattcaatgc
      241 tttagggatt gtttatggtt tttcgaaata tacaagagaa gatggggtcc acccaacgat
      301 tggcggacat gagatcattg cacaaagctg gatagatgca attgccgcag tcgatttttt
      361 tagtggagat aaaaatgagc gaaagaaaag tgagaaatga tggaaatacg gtgggcagta
      421 aaagaagatc tacctcaaat caatcagctg attactgatc atccagaatc attcgtaaaa
      481 gcaaaaacgg cagctgacct aaatgataag aataaactat tgctcgttgc tgtaacaaaa
      541 gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac agtcagaaga acgattggat
      601 cagcgaccat caggaaaact aatccaacac acattagtaa caaaagaaaa tacggaagaa
      661 atccaaaaaa aatttgcaga atatttgata ctggaagcta aaaaaagaag aataaaatca
      721 ttttttgccc agttttaatc aaaaacagta atagaaggag aaatacaatg agttgtttag
      781 gaaatgtcat ctggtttata ttcggcggac taatcggtgg tcttagctgg ttagcagcgg
      841 ggatactttg gtgtatcacg atcataggta tcccaatcgg tatgcaatgt tttaagatcg
      901 cctccctttc ttttgcgcct tttggcaaag aagtaattta tcaccagtct ggcatgtcat
      961 tgattgttaa tatcatctgg ctgatcgttt caggcctacc aattgctctc gcacatgtcg
     1021 tcagcggcat ccttctatgt atcacgatca tagggatacc ttttgccaag caatctttca
     1081 aacttgctcg attggcgttg atgccattcg gtgcagaaat tgtataaaag agaataataa
     1141 aggttagata aagcatagag tcaggtggga tacatgtatt tcacctgact ctatgcgttc
     1201 tcctgtataa gttcttccat gtgactttta cgtcttttat ttatatgggg ttactgttta
     1261 tggaaagttg aaataatttt cattaaagaa ctctttctat atttttgaaa gcgtagtgga
     1321 gcttcgaata tctgggaagg acattttgca gggattgaaa aaagatattg tcctatcgaa
     1381 aaattaatga atttagacaa attgccagca acaggcttca aacttagttg ctatcctgtc
     1441 aagatcaaga aggcaagtgc gggttggata agagcagtgg caatgatcga aaagaaaaag
     1501 aaagaataag ccaaaacatc tgtatggaag aaattttccg atgcagatgt ttttcatttt
     1561 ttctttcgga taccttgaca tatgaataac cgttcattta taattagtta tgaagagata
     1621 ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt acagattaga aggtttagat
     1681 tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga agatcaatgg agtcaaggaa
     1741 gcaacggtaa acttcacttc tggaaaacta acgattgacg cagaggaaga tcatttggct
     1801 acgattgaac aagaaataaa aaaagtagtg aaggaattag aaccagatgt caaagtaaca
     1861 gaaatagata aggaaaaagt atctgaacat ggaaatgaaa aagagagaaa tacgctgttc
     1921 cggattttat tttcccttgc aggtatcgct ttattgctat tatttgattt taatgaacca
     1981 attcgcttga tagggtattt attgatttat ctgctgatag gatatgatgt agtgaagaag
     2041 gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga attttcttat gtctgttgct
     2101 acgattggcg ctatgttgat cggtgaatat ccagaagccg tagcagtgat gctattttat
     2161 caaatcggcg agtatttcca aggattggca gtcagtcatt caagaaaatc gattcgcgaa
     2221 ttgatggcga tccgtccaga aacagctcac gtgcagacga cggaaggatt gatgaccgtg
     2281 aaccctgaag acgtgcagat cggtcagttt gtacttgtga agcctggtga acgtgtaccg
     2341 ttagatggaa cgatcatcga aggagaatca ttagtcgata cgtcagcact tacaggagaa
     2401 tcagtcccta aatctgtata tgttggtgaa acagtattaa gtggatttat caataaaaat
     2461 aaaccatttt tagttcaagt agaaaaatct tatgaaaatt ctactatcag caaattatta
     2521 gaattagtag aaaatgccag cagtaaaaaa gcaccagctg aaa
//
LOCUS       DACENJ010000248         2559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.256, whole genome shotgun sequence.
ACCESSION   DACENJ010000248 DACENJ010000000
VERSION     DACENJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.256"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(512..1069)
                     /locus_tag="IW748_002479"
     CDS             complement(512..1069)
                     /locus_tag="IW748_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105373.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1248..1859)
                     /locus_tag="IW748_002480"
     CDS             complement(1248..1859)
                     /locus_tag="IW748_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105374.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(1972..2229)
                     /locus_tag="IW748_002481"
     CDS             complement(1972..2229)
                     /locus_tag="IW748_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105375.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
BASE COUNT          763 a          541 c          333 g          922 t
ORIGIN      
        1 aagctaagca aattatctcc accaaataat ttattcatat ttttgacttg cgcatactaa
       61 ctaattctta aattttcata caaaatttct caatttccct taacgagtaa tatttatgta
      121 cggtgattct cactagtatc ccaatcactt tttaagttat cccgatttca attctacttc
      181 aaaaatagtt gcttttatgt atgctgttag ttgattagat ggtcatttgt tgagctaggt
      241 cgtttatttt aaacaagttg tttctttagc ttatacctat ctcgtctacc aaacgcaaga
      301 tggcaaacac aaatgcatac gcatctgtat ttgccacttt gattcgtata aagctaacaa
      361 ctattaactc ctctcagcta atagttgctc tcttttttat tcagattcat ttttgctgat
      421 cacagcctag tatttcaatg gttttgaaca ctaaaccaaa ttgttccatt acaacattca
      481 ctggctgatg aaatggatta gtagatttcg attattcaaa gcgattttct attggtggat
      541 cgtgttccca actaataaga cggattccaa acctatcgta cagatcttct aaaccactta
      601 taaattttgc acctatttca ggtgttttat tgcgtggata ttctattgtt acgccattaa
      661 atggatacca attaggatta ggctctcttt ttttctgtac atctcccata gaatagttag
      721 cactttctgg gacataatat attcttcgac catcctctaa ttcatctaat aagatatttc
      781 gtgatcgtct atcacttgct aaatgtagac gccacgtgcg ataccatgcc atgctacctg
      841 catctgcaga aaatccatca aataatgcaa caattgtatc ttctatcaca atcctttccc
      901 catctacttc atattctaat ctaaaatcaa actcccctcg ggtaatttct ggtcttggtg
      961 gcttaggacc caatattatt ctacttaaaa ccgcattcgt tcccagatga aacacagcta
     1021 taacaacaat aatgattaat attttgtttc tatttgataa atttctcatt attcgcacat
     1081 cacctctaca ttctcacata ttagttcgta atactccttt aatatcaaaa tatctactgg
     1141 tcgtaataat attcgctatg aattcctact actcgtataa gaaaggccaa atcatcagct
     1201 tttacttgct gatgatttgg cttgtttcta tttatcgtaa ttgttttcta atcaccaaaa
     1261 ttgttaacaa tcggttcaga tatttcccag ttgattaagg taattccgta ttgtgtcaaa
     1321 acttcttgcg cttctccgaa attaagtaca gttcttatgt atccactttc tcctaatata
     1381 aagtctacct ctcttttaaa accttcctcg agatttctaa gggctatatg tggttttaat
     1441 gacattgatt gtacattttc ccccatataa tattgcgcac ttcctcgact aaaatagatt
     1501 cctagatcgt ccccaaaagg tagatcttct ctaccactag ctaatctact tctccatctt
     1561 cgttctcttt tcatagaacc tgcgctgaac tcacacccgt taaactcagc tatgacagta
     1621 tcttcaatta tgttaatctg tccatctagt tcgtattcaa tcctaaatgg aaattctcca
     1681 taggtgattt ccggttcggg tattttaggt gagattaatg caaagaaagg aaatattaag
     1741 cttggcaaca tcaaaaaaac tcctcctatg atcacaattc ccgatgtaat ccaaaatata
     1801 atttgtacaa ttctcattga tccattgtat tccattttat tccccctatt actattcata
     1861 tccgcctatc tatatcgatc ttccaatgca gagataattg cttccatacc ttgatttttt
     1921 actgagttac ctatgcgaga tatcattctc cactaaggtg catcatattg tctatctaac
     1981 atgattaatt ctccaatagg tgtatactcc tccaaaaaca acatttaatg catctccctc
     2041 gacaacataa gctaccatgt ttgctgtata tgtgtttgag tctaattcat aagcagataa
     2101 atttactgtc gctggattaa atataatcgc attcgtattt atagctactg aatttgctgc
     2161 ttcagcacca ccttttgaat gtccgatcat tgtgatttca atagtaggat ttacgtggac
     2221 agccctcacc gtttaccctg accattatag aaatcgctta cttttttgaa ctacgcaaca
     2281 aaggatacaa tgctcctaac ttcactgtct ccatttttgt ccactaagga atataaaaaa
     2341 taggcattaa ttcatttaat tcggtcacaa cacgttacct tttaaatcat tatactgtat
     2401 ctcatacatt attatcaata aactattata aaaatgcaaa aaatgcttta tcaatctatt
     2461 ttgtaattgg tcataatatc attgaaatca taccctgtat cgatttttcc tatgaatctc
     2521 gtctatttgt gattgacgaa aaagaggcaa gacagacac
//
LOCUS       DACENJ010000249         2548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.257, whole genome shotgun sequence.
ACCESSION   DACENJ010000249 DACENJ010000000
VERSION     DACENJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.257"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            52..1698
                     /locus_tag="IW748_002482"
     CDS             52..1698
                     /locus_tag="IW748_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1105376.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            1747..2466
                     /locus_tag="IW748_002483"
     CDS             1747..2466
                     /locus_tag="IW748_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1105377.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
BASE COUNT          774 a          431 c          567 g          776 t
ORIGIN      
        1 tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc gatggataat
       61 caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg aggctctgct
      121 tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat cattccctgg
      181 tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat gggatacaat
      241 atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat tgcaaaacaa
      301 actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt tatccgtgca
      361 ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat cgcctctcct
      421 tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg ttctttgagt
      481 atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca aggcaatcaa
      541 gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat tttttatatg
      601 ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac ggcggtcacg
      661 caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct tttgtatttt
      721 ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa caaaataaat
      781 attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt tattattgtg
      841 ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa gatcatgtct
      901 gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt tagtgccaat
      961 ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc tactgggtta
     1021 ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa attgacaagc
     1081 ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt tatgttactt
     1141 gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa tgtactgatc
     1201 caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa catgctgcaa
     1261 ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct agtgaaactc
     1321 cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt ggcgacaatg
     1381 atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt agcccgcttt
     1441 aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt gattatgctt
     1501 ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga tagcaaattc
     1561 cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta tggttattta
     1621 gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg tttacgacat
     1681 aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt gaaatggggg
     1741 aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg caaagaagtc
     1801 aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa aagcgataaa
     1861 gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat tacgtatcaa
     1921 aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac tcaggataat
     1981 catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga tctttttcca
     2041 gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga cggggtgttt
     2101 gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc tgaggttagc
     2161 ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat tgatggcgat
     2221 gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac agaaacttca
     2281 attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat ggtcaaagca
     2341 gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg gctgcctaaa
     2401 gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca agtgagagaa
     2461 aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca tctgaaaagc
     2521 agacttgtcc gtctgacttt cggatggc
//
LOCUS       DACENJ010000250         2527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.258, whole genome shotgun sequence.
ACCESSION   DACENJ010000250 DACENJ010000000
VERSION     DACENJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.258"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..507
                     /locus_tag="IW748_002484"
     CDS             <1..507
                     /locus_tag="IW748_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105378.1"
                     /translation="GMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFYQLEAEN
                     LSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFFVWLDLL
                     GYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRTMTVFQQ
                     IVSEIRGN"
     gene            509..1768
                     /locus_tag="IW748_002485"
     CDS             509..1768
                     /locus_tag="IW748_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1105379.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            1774..2355
                     /locus_tag="IW748_002486"
     CDS             1774..2355
                     /locus_tag="IW748_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1105380.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
BASE COUNT          895 a          395 c          521 g          716 t
ORIGIN      
        1 ggaatgattt gtgatcatcg ttttttgcaa aatctgtata aaaactatcc aaataaaaag
       61 caattaacga ctcggccgtt tgccgggatg atcgaagaaa tgatctattt ttatcaatta
      121 gaggcagaaa acctgtcaaa ttatggtaaa ctgtttgaac aagcttttta tctgaccgaa
      181 aatggccatc tactttctgc tagtatgaaa aaatttttag gaatgatgtt tcatttcgtg
      241 tggaatctcc aagcatatcc ttttgatttc caactaaaaa acttttttgt gtggctggac
      301 ttgcttggct atccaatcca ctggctggaa cagatttcaa agatggagta tttggaaaaa
      361 gttctttttt tggcagaaaa aagcaaaaaa atacttgttt tagaaaaaat aggtataata
      421 gacggatacc aacatccaaa cgaaaaagaa ttattgagaa caatgacggt atttcaacaa
      481 atcgtcagtg agataagagg gaattgaaat gaatacaaat agcgaaaaaa tgttgcaagc
      541 tttatcagat gaagacttgt cacaagcaca aatcttttta gaaaaagcat taaaagaaga
      601 cccagcagat atattagctg aactaggcga agaattgctg gctattggct ttttgatcga
      661 agcaaaacag atttttgaac aactgatcaa ccaataccca gaaaatgacg gactgaacat
      721 cccgttagca gaaattgcaa tcgaagataa tgagatcgac ttagcttttg aatacctgga
      781 aaaagtggat aagcaaagtg attactatcc gcaaagctta ttggcgatag ctgatttgta
      841 ccaagttatc ggaatccctg aagtgagtga agcaaaattg aaagaagcag ctgggttgct
      901 tcctgatgaa ccattgatcc agtttgcgct agctgaattg tatttttcag ttgatcgttt
      961 caaagaggca gcagtggttt atgaaacatt gttggcaagc gggataaatg aaatttcagg
     1021 aatctcgatg caggaacgtt taggacaatc cttaagtatg caaggagaat tcgaagcagc
     1081 tatcgtacct ttagaagaag cactgaaaga agaaaggaca gatgatcgac tgttccagct
     1141 agcttttacg aatcttcaat tgaaagaaaa tcagcaggca atcaactatc tggaagaact
     1201 tcgagaagtt aatccgcatt atcaatcgtt atatctatat ctaggacaag ctcttcaaga
     1261 agaagaaatg attgaagaag cacaaacagt gttagaagaa ggaatcaaag aaaatcctta
     1321 ccaagtcgaa ctttatcacc tggcctcgga aaatgctttc cgtctgcatg atagacagcg
     1381 agcagaagaa cttttactga aagctttaga agtaggagac aaacaagatg aaacattact
     1441 tactttaagt aatctttact tggatgatga aagatatgaa gatgtgatac aagtgatcaa
     1501 tcagatggaa gaaactgcta atccttatgc agaatggaac ctagcccatg cttataatga
     1561 attagaagac tttaccgttg cagctgtaca ctatgaacag gcttatcatg aactatcaca
     1621 tgaaccagat ttcttgaaag agtatgcctt tttcttaaga gaagaagggc agctgaaaag
     1681 agcgcaagaa ttattgactt attatttaac acttgaacca ggagatatgg aggcactgtc
     1741 attattagat gacctaacag agaggtgaga aaaatgctaa ttgatgtacg ggaaaagaag
     1801 aattttttaa cgtggatggt caatcatgta tcttttagcc gaagagaagt tttttggatc
     1861 ttgaattatc ttgccaatca tgaagcgatt ttaagcaatg tccactttgt tgaaggggcg
     1921 aattttgcgg atcgaggact gcagatcagt gatctatctg ttgaagggga acccatgaaa
     1981 ttgtttttgc agggaaaaga atttacggac acagaccaga tttttcatga aatccgtttg
     2041 aattggaaaa agcctttata tgtggaatgt atattcgaaa atgcttggca gacaagagaa
     2101 tatctatcta tcttagaaga caatccattt tcttcttggg attcttcagt cagtgaagaa
     2161 gatactgaat taattgaacg atattttcaa gaaaaagaag aagaggcaaa actaaaaatg
     2221 ctttatgctc aaatcgatca agcactagaa gatggagata gggatgcttt tttgaaatta
     2281 tctgatgaag ttaaccgaaa gatccttttg cagacacaaa agaaaagtaa ccgggtaagt
     2341 gaacataaca attagttaaa aatagtatag tactaaaaaa gacaaaggtc agctggatgt
     2401 ggaatctttc ctcatagcct atctttgtct tttttcttat agttttttca gtaatcttgt
     2461 ctgtggcttc aaataagtaa agccttgccc ttccacttca tgcacattga tgattgaaag
     2521 gaaagct
//
LOCUS       DACENJ010000251         2514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.259, whole genome shotgun sequence.
ACCESSION   DACENJ010000251 DACENJ010000000
VERSION     DACENJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.259"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            69..2483
                     /locus_tag="IW748_002487"
     CDS             69..2483
                     /locus_tag="IW748_002487"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1105381.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT          861 a          459 c          559 g          635 t
ORIGIN      
        1 attctgccgg ttttttcgtt atatttataa tagaaacaag tgttttcaat agaacaggag
       61 gacagcaaat gagttacaat cataaagaaa ttgagaaaaa atggcaaaaa tactgggcaa
      121 aaacgaatac tttcaacacc catgatgatc ctgaaaagcc aaaattttac gcactggata
      181 tgtttcccta tccatccggt caagggctac atgtagggca tcctgaaggt tatacggcaa
      241 cggatatcct ttcacgtgtg aaaagaagtc aaggatacaa cgtgctgcat ccaatggggt
      301 gggatgcatt cggtctgcct gctgaacaat atgcgctaga tacaggaaac gatccagctg
      361 agtttaccca aaaaaatatc gaaacattcc gacgccaaat caattcttta ggatttagtt
      421 atgactggaa tcgcgaagtg aatacaactg atccggagta ttataaatgg acacaatgga
      481 tctttacgaa gctttatgaa aaaggtctag cttatgaagc agaagtagcg gtaaactggg
      541 tacctgaatt aggaacagtt atttcaaatg aagaagtgat cgatgggaaa agtgaacgtg
      601 gtggctatga tgtagtcaga aaaccaatgc gccaatggat gctgaaaatc actgcttatg
      661 ctgatcgctt attggatgat ctagaattag ttgattggcc agaaagcatt aaagaaatgc
      721 aaagaaattg gatcggtcgt tcagtaggag caaatattga atttaaagtg gctggtacgg
      781 ataaatccta taccgtattt acgacacgac cagatactct attcggtgca acttactcgg
      841 ttcttgcacc tgaactagat cttgttagag aaattacgac ccctgaacaa aaagctgctg
      901 tagaagctta tatcgaagag acagctaaaa aatcagatct aaagagaacg gatcttgcaa
      961 aagaaaaaac aggagtattt actggtgctt atgcaatcaa tccagttaat ggcaaggaaa
     1021 tcccaatctg gatcgccgat tatgtattat cttcatacgg cacaggagcc atcatggctg
     1081 ttccagcaca tgatgaacgt gactttgaat tcgctcaaac tttcggacta gaaattcttc
     1141 cagttatcga aggtggagat gttgaaaaag ctgcttacac agaagatggt acgcacatca
     1201 actctgaatt tctagacggc atgaacaaac aagaagcaat cgataaaatg aatgaatggc
     1261 tagaagaaaa tggcgtaggg aaaaaagaag tcagctatcg cttacgtgac tggctattct
     1321 cccgtcaacg ttactggggt gaaccaatcc caatcatcca ttgggaggac ggaacagtca
     1381 cagctgtccc agaagaagag ctaccattac ggttgcctaa gacaaaaaac atcaagccaa
     1441 gcggtactgg agaatctcca ttagcaaata tcgaggagtg ggtcaatgtc gttgatcctg
     1501 taacaggtaa aaaaggccgt cgcgaaacga atacaatgcc acaatgggca ggaagctcat
     1561 ggtattacct gcgttatatc gaccctcata acaaaaaaga actagcagat tatgaaaagt
     1621 tgaagcgttg gctaccagtt gacatctaca ttggtggagc agaacatgcg gtacttcatt
     1681 tactatatgc acgattctgg cataaattct tatacgatat cggtgttgtt cctacaaaag
     1741 aaccattcca aaaactatac aatcaaggaa tgatcttagg cgaaaacaac gaaaaaatgt
     1801 caaaatctcg tggtaatgta gtcaatcctg atgatgtggt cgaaacttac ggtgcagata
     1861 cactgcgcat gtacgaaatg ttcatggggc cattagatgc ttcgattgct tggagcgaaa
     1921 atggcttaga aggaagccgt aaattcttgg atcgtgtatg gcgtttgatc gttgatgaga
     1981 acaataaaat gcgtgaccgt atcactacat tgaatgatgg caagcttgat aaagtgtatc
     2041 atcaaacagt gaaaaaagtg acagaagact atgaaaatct tcatttcaat acagcaatct
     2101 cccaattgat ggtctttatc aatgaagcct ataaagtgga cgctctgcct tatgaatata
     2161 tcgaaggatt tgttcaacta ctcgcaccga ttgctcctca catgggtgaa gaactatggg
     2221 caatccttgg aaatgatggt ggtatttctt acgcaccatg gccaacatac gatgaagcag
     2281 cacttgttga agacgaagta gaagttgttt tccaagtaaa cggtaaagtt cgtgcgaaaa
     2341 gcaatgttcc ccgagactta ggaaaagatg agctagaaaa agcagcatta gccaatgaaa
     2401 tagtgcaaga atatattgaa ggcaaaacag tcagaaaagt tattgcggta ccaaacaagt
     2461 tagtcaatat cgttgctaat taattggctc tttgtcaata aggactgatg agtt
//
LOCUS       DACENJ010000252         2499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.260, whole genome shotgun sequence.
ACCESSION   DACENJ010000252 DACENJ010000000
VERSION     DACENJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.260"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..598)
                     /locus_tag="IW748_002488"
     CDS             complement(<1..598)
                     /locus_tag="IW748_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105382.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPV"
     gene            complement(755..1414)
                     /locus_tag="IW748_002489"
     CDS             complement(755..1414)
                     /locus_tag="IW748_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1105383.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(1417..2442)
                     /gene="sppA"
                     /locus_tag="IW748_002490"
     CDS             complement(1417..2442)
                     /gene="sppA"
                     /locus_tag="IW748_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1105384.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEKGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
BASE COUNT          694 a          565 c          410 g          830 t
ORIGIN      
        1 aaacaggatg gcattcgttc aattgcctaa gtgtctccag taattttgcc cttgctaaaa
       61 tatggacctc tggcgataca ataagtttac tcgctgtttg gataaactca tgataaggaa
      121 gctgttcgat ctcatcaata gacgcaagct gacgtgcagg ttccaacgaa ggaagttcct
      181 ccgatttatt gtggagtgcc ttgatcttct gcctctccat ttgtccggat aattcttctg
      241 caaattggat tttttcaatg acccgctgct cgaaagcttc ataaaaatct gtacttgcta
      301 tcaattttct agcatataaa aaatcttgtg tactgattaa caagtcaaaa taaaattcag
      361 caaactcctc attttgaaga tactgttttt catgcaagag tgcttgcttc aatgcttctt
      421 ttttctcgtc caactcgtaa agacaaaaaa caagcaggct attagcctca aagctttctt
      481 tcaactcgta agctcgttga aataattctt ttgcctgctg atattgctgg tttgctagtg
      541 cttgttgtgc cactcgcatt aaattctcat atttttctgg aaaatcaatc ggcttcacgc
      601 agtgtcctcc tcttgctcat attcagctca tgttcaatag tagcaaaaca aaaaaagcat
      661 gacaattaaa aataactgaa gcaccctcta aaagctagat tttagatctg acttttagaa
      721 ggtgctttca actgttactg ctttttattg ctcatcagct gttgacttcc tggttgaacg
      781 ctttgatcag attgatcgta acaacacttg tattagtgaa aaaatcgccc agatgttgct
      841 ttttctgatt aaagactgct ggaagatacc ctaacatcag caaaggaaat tttagaatga
      901 accggcaagc accttctcta actaaaacgg ttgaccagct aagttcttct tcatcaaagc
      961 tgatgactcg aataccaaag atcattttcc caatcgtttg gccatgattg aatttcgtca
     1021 ataaaataaa atacgctaga taaataagta aggctgtgaa tccgtagaga ctcagataag
     1081 aatctgaaaa attaagccaa cctagatttt tggcaatacc aagtgttaaa cgcgtaatag
     1141 ctccgataca caaaagatcg accaagaagg caaacattcg gataaaaaaa cctgcataga
     1201 aatacttagg aaaatcattt gcttttggcg gttcatttct tgtgtccgta tactttttcc
     1261 atttacgttg tttctttttg atttcttcgg gcgtcaaagg ctctctttca aaagagctca
     1321 gtaccttttg ggaaaagttg gagtcaggtc ctacagcagg ctgctgttgc ttatcttttt
     1381 cttgttcctt attttcattt gaaagctctg acatgcttac tcacctccgt aataatacat
     1441 tgcttttggt gattccgctg tccctagatt ctcaagaacg gataagatct gactagtctc
     1501 agaaggcttc aatccctgga attctgctag tttacttcct aaccatgtac tagcaaaccc
     1561 agatggcgtg gaagaatatt ctactagttc cgcatcttca agctgttgtt cctttcgtat
     1621 agcttttaaa gcatcttcag gaaatcccaa ttcatcgact aaacctactt cttttgcttg
     1681 gacaccatca taaatccttc catcagcaat ttttttgact tcttcttcag atttattccg
     1741 tccctcgctg acgattgcta caaaacgact atatgcgctg tcgatatatg cttgaagcac
     1801 cgcttcttct tcttttgtct ctggtcgcgt tcctgaaccc atatctttca acgctccgct
     1861 tttaactgtt gtatcttcca cacccagttt ttctagaagt cctgaataat tcaatcctga
     1921 catgatcaca ccaattgagc cggtcactgt ttcttctgtt gcaaagattt tatctgcttg
     1981 agcagaaata tagtagccac cgctagctgc catgtttttc atgctgacat acattgggat
     2041 atcatgttct ttacgaatcg tatccaattt cttagcgatt tccgcacttt cgtagacacc
     2101 gccaccagga gaatttactt ctaaaaagat acctttgact gttggatctt cttcaatcgc
     2161 cttcagttgt tccataaaat ttttatgatt gtaaccttca gaagcaaata aacctgaaga
     2221 ccctcccgca gcaatcgttc catctactga taattttacg attttttgcg tactgtctcc
     2281 cttttccaac acatttggag ctagttcttc atccccatat aaccaactgt tcaatccctc
     2341 aatttgatcg ttttcctgag gcttagtcaa actagctgaa actaacgaaa ccacaaataa
     2401 tcctgcagca atcagaactg ctgcccaacg tcttttattc attctttctc ctttctaata
     2461 caagtttttt atgacgatca cagtttctga ctgctcaga
//
LOCUS       DACENJ010000253         2469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.261, whole genome shotgun sequence.
ACCESSION   DACENJ010000253 DACENJ010000000
VERSION     DACENJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.261"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..502
                     /locus_tag="IW748_002491"
     CDS             <1..502
                     /locus_tag="IW748_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ1105385.1"
                     /translation="QGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPK
                     KNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTV
                     QGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRK
                     YINFDK"
     gene            complement(632..2071)
                     /locus_tag="IW748_002492"
                     /note="aph(2'')-Ia"
     CDS             complement(632..2071)
                     /locus_tag="IW748_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ1105386.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(2072..2464)
                     /locus_tag="IW748_002493"
     CDS             complement(2072..2464)
                     /locus_tag="IW748_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1105387.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          846 a          433 c          231 g          959 t
ORIGIN      
        1 acaaggtacg gaactcgtta tttctgatgc gcacaaagga ttagtctctg ccattagaaa
       61 atccttcacc aacgtaagtt ggcaaagatg ccaagttcac ttcctaagaa atatctttac
      121 caccattcct aaaaaaaatt caaaatcttt cagagaagct gttaaaggaa tttttaagtt
      181 cacagatatt aacttagcgc gtgaggctaa aaatcgattg attcatgatt atatcgatca
      241 accaaaatat tcaaaagctt gcgcatcatt ggatgatgga ttcgaagacg cctttcaata
      301 taccgtacaa ggaaattccc acaatcgact aaagagtacc aatctaattg aacgactgaa
      361 tcaagaagta cgcagaagag aaaagattat tcgcatcttc cccaatcaaa catcagccaa
      421 tcgcttaatt ggagccgttc ttatggacct acatgatgaa tggatttatt cttcaagaaa
      481 atacatcaat tttgataagt agaaatggta aaaacattgt atagcatttt acacaggagt
      541 ctggacttga ctcacttcct ttattatttt tcattttttt aaactattta atactaatgt
      601 cttttataat agcttttcat atattatata atcaatcttt ataagtcctt ttataaattt
      661 cttttctacc attttcgata aattcctgtt taatattttt aattccataa acaatagttt
      721 caataggata atattcttca actatatctt gatattcttt tgctttctca atatctatat
      781 ttccatacat tcttaatata tcttctccaa aatttgttcc tatttcttct tcactatctt
      841 caagtaagta tataaaatca caatattcat ctataattcc agaatctcca aaatcaatta
      901 ttccagttaa tctattattg ccatctaaca atagatgatt acaactaaaa tcattatggc
      961 ataaacactt tttaccctca aaaactgttg ttgcatttag tctttccata aaactttcta
     1021 tataatcttt ttctatatca gttaaatcat tataaatagt ttcacgcaac aatatatact
     1081 cttctaatac attttgttta ttatcaatag tacattcact aatatctgta taatctaaac
     1141 cgtgcatttg tcttaaaaaa ctggcaatat ctcgttttaa caaattttgt tcttcttctg
     1201 acatagtaga ataaatttct ggtgttaaaa aagttccttt aatttcttta taacctagta
     1261 tagataattc atcactaata tacgaatatt caatattagg aatttttaca ttagtttcta
     1321 aatttgtatt taaaaaatta tatattgctt tttcttttgc ataacctttt ttcttattag
     1381 tactaaattt tgttttaaaa atgtattcat tattaactaa atatgccaca ctatcataac
     1441 cactaccgat tatttcaata ctatctactt tgaaattatc aaagtaatgc tcaattaaat
     1501 atttcattgc cttaacattt gtggcattat catcatatct atattccatt aaataacaat
     1561 cttctttttt gccctcgtgt aattcatgtt ctggcaaatc ttcaataatt ctaaaaccag
     1621 atttttggta tgcccttatt gctcttggat tatttttatg agggtctaaa ataactgcat
     1681 tagcatttct ttcttttttc aaaaattcaa aaatcaattt aatatatctt gtaccaattc
     1741 ctttactcca ataatttggc tctcctataa attgatccat accatagact atctcatcag
     1801 tttttggata atgataatca gtatataact catcatacat tttatatatt tgtccatatc
     1861 caataggaac attgttatat tcaataatta ctctaaaaac ttcatcttcc caaggctctg
     1921 tataatgttt ttttaatgat tctaatgtat attttttatc tctaccacca taaaattcta
     1981 atactctttc atcagttaac cattttaaca tcaaaggaaa atcatcatct attaaagttc
     2041 ttatacatat ttcattttca actatattca tttatttatc acctttttca taatcatata
     2101 catatactat ttcatcttta taatcatttt taccacctaa tttttcatat acatggcaag
     2161 ctctaggatt acctttatca gttattaaaa acatttcaga acaaccaatc tctttagaat
     2221 attccttaat aaaagataat aattttgaac cataaccttt gtcttgatag ttaggtaaca
     2281 ttcctattga gtgtaaataa aacattgttt ttccatcagg tcttaaaagt gtatagcaat
     2341 atgcaaatcc tataatttta ttattttctt tagctataaa cccaaatgaa ctggtatcat
     2401 taagaaatcc ttttaaatta tcaatatcaa aaaccatatt atcatcaatt aaaacttctt
     2461 ccataaact
//
LOCUS       DACENJ010000254         2434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.262, whole genome shotgun sequence.
ACCESSION   DACENJ010000254 DACENJ010000000
VERSION     DACENJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.262"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            81..377
                     /gene="yidD"
                     /locus_tag="IW748_002494"
     CDS             81..377
                     /gene="yidD"
                     /locus_tag="IW748_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1105388.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            562..1029
                     /locus_tag="IW748_002495"
     CDS             562..1029
                     /locus_tag="IW748_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1105389.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            1114..1998
                     /locus_tag="IW748_002496"
     CDS             1114..1998
                     /locus_tag="IW748_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1105390.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            2115..2348
                     /locus_tag="IW748_002497"
     CDS             2115..2348
                     /locus_tag="IW748_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1105391.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT          879 a          343 c          545 g          667 t
ORIGIN      
        1 atgataaaag aaaacagaag ggaacgttga cgttagtcgc gatccctttt tttagtaaaa
       61 gaaaagaagg aatctaattg atgaagaaga tattgattta tttagttaga ggatatcagc
      121 gctttatttc gcctgcattt ccacctagct gccgttatta cccaacctgt tccaattata
      181 tgataaaagc aattcaaacc catggcgcgc taaaagggac ggtaatgggg actgcacgga
      241 tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta tgtaccattg acattccgat
      301 taacgcgaaa taagaaagat aaaaaagaac cagattataa aaaggttgtg aaaaatacgg
      361 gtagcactga gcgataaaga aacaatttgg aaaatagctt cttatatttt gcaaaattgt
      421 gagttattgc cgaagtgttg gattttgaac accgtcaaaa aaaaggttgt gaaaaatacg
      481 gctagcactg agcgataaag aaaaagagga aatttattgt caaagtaaca actccgcaac
      541 tacttatatg ggggacaaaa aatgatcgaa gaactaaaca agaaagattt accatggtcc
      601 ttgttattag atgcagatcc tgataaagaa aaagttcaag tatatgtttc cagagggagt
      661 ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa
      721 aaagaatacg aaatcgtcaa tgtagcagta accccaacgt atcaaggaaa ggggattggc
      781 ggaaaattac ttgagacagc ttttcagaag ctttcacaac tcgcttccag tcaaactcag
      841 atcatcatac gaaccggcag tactagcagt gcggcgttac atctctatca aaaaatgggc
      901 tatgtggaga ttggtcgagt caaagactat tttatccatc attactctga gcccattttt
      961 gaaaatggag atcaattaag ggatcaagtg attttagcaa ttcacttacc taaaaatagc
     1021 tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa tgctaaatag atacattaca
     1081 atagctttag caaacaaaaa ggagaatatt tttatgggaa tattagtttt tgattttggt
     1141 ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt
     1201 gcaacgcctg aatcttggga agaaatgaaa gcgcaattat ttcaagttta taaaaaaata
     1261 ctcgtttctt ttgaagtcga aggaattgga atcagctctc ctggagtagt gaataatgag
     1321 aaacaggtga ttgagggaat cagtgcgatt ccttatattc atggatttaa tattttccga
     1381 gatttggaaa ctctgtttca acttccagta acgattgaaa atgacgccaa ctgtgccgga
     1441 atggctgaat tttatgaagg tgctgcccgc gattatcaag atgttgcttt tgttgtggca
     1501 ggaactggga ttggcggggc gttgttcacg aaaggacaaa tcaataaagg ggcacatctt
     1561 tacggtggag aatttgggtt gatgttctta aatggcgata agacattcag taaacttggt
     1621 acagcggtac agatggcttg gcgttattgt gaacgcaaag gtctcgataa aaatgcttat
     1681 accggaaaag atgtctttga attagcagaa actggagatg caatagcaaa agaagaagtt
     1741 gagagttttt atacgtatct tacaaaggga ttattcagta ttcaattttc gtttgatcca
     1801 gaagtgatcg tattaggcgg cggggtttct gcaaaagaag ggctcattga tgagatcaaa
     1861 agccgaatga agaagctgac agaacaattc gatctgcatg atttcgaacc gcagatttta
     1921 ttatgtgaat acagaaacga tgctaatctt gtaggtgcag cagcaaacta tctcgctgta
     1981 cgtcaagggg agaattaaga gctttagaga aggtgcagct tgcataaaag aagtgaatga
     2041 aatgaatagg agatttttac ctaaggaatg ttttatttgc tgcagtatga taaaatatct
     2101 gagaggtgaa aatgatgtca aaaaaaaata aagatattga agtgcgtata gaagaagtga
     2161 aaaaagatat caaaggtaag aattatgaag tcactcagtt atttataggg aaaaaaatga
     2221 ttggtgagat cttagcctat ggaccaaaag aatatgaaat tttctttggt gaagaagatt
     2281 ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt gattcgacac tggaatctgc
     2341 acgaataaaa aaatatcaaa aaaactttca ataaagggtt gcaaaaatct ggaaaattag
     2401 gtattataac taagtcaggt tgatacaaac cttt
//
LOCUS       DACENJ010000255         2422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.263, whole genome shotgun sequence.
ACCESSION   DACENJ010000255 DACENJ010000000
VERSION     DACENJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.263"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            42..683
                     /locus_tag="IW748_002498"
     CDS             42..683
                     /locus_tag="IW748_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1105392.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(745..2376)
                     /locus_tag="IW748_002499"
     CDS             complement(745..2376)
                     /locus_tag="IW748_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1105393.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT          772 a          540 c          443 g          667 t
ORIGIN      
        1 tttcttttat tcttataata aaagaaaaag gaggagcata aatggaatac ccaaaagaac
       61 tgatacaaga agcttcttta agaatgaacg gccgtcctgt agaaggattt atcgctgggc
      121 aaggacctct tcatcctaaa ctgatgctcg tgggagaagc cccagggaaa acagagattg
      181 atacgcatgt tccttttagc ggtcaagctg gaaaagaatt gatgcaggca ctttcttcaa
      241 ctggcttaac acgagaagag gtgtatatca ccagtgctgt aaggagccgc ccgtatcgcg
      301 tgactcatcg gatcaataaa cggacacaac agtcagaaac tgtttatcca aatcggacac
      361 ctactcgttc cgaagtattt gcgcatgcac caatattgga ttacgaactg atgaaggtcc
      421 agccaaaatt aattgcgaca cttggaaaca ttggtcttca acgtttactt ggcaaagaat
      481 atacggttac caaaaatcat ggtcaactct atacagggcc tgtcttacaa ttaacagaac
      541 aaaaggatgc ctacgaatgg tcagaaaaaa actatcgaat tcttccactt tttcatccag
      601 cagctatttt ctacaatcgc tcccttgtac ctgttatcca tcaagactgg caagtactag
      661 cttcactttt acagcaatat taaaaagaac agacaagacc ataaaatctt cagtttacag
      721 tcttgtctgt tctttttcat tgttttaatg gctaattgca gtatatttcg catgtaaagc
      781 tgtcaacgca tgagcaactg tttgatgttg atgcgtgaat gtatgttccc acaagtgaga
      841 aagttgtttt tctacttctg gctgcaagga agtatagata atcgtttgtc ctctttgctc
      901 tgctttttct tgaaattcat caaaaactgc acgtgccgtc aaatcaatac ttggtacgcc
      961 tcgcatggag aaaatgacac catcgtgatg atttagttct tccaatttat ttttgagttt
     1021 ttctgaagac ataaaaaaca gtggaccact aatatacact actgtccagt tattcagctt
     1081 ttccgtttct ggcagcccca ttctttgcca atcgatcgtt tctaatgaaa tcgtgatagc
     1141 agcgcttttg acaacaaaaa atacacagcc gccgatcacc ccgatcacga tagcaatact
     1201 taaatcaaag ataactgtac aaatcatcgt aaggataaac aatagaactg ctgaccaata
     1261 tttattagta aatagctcct tgatcgtttc ccattcattc atacgccaag ccgtcacaat
     1321 caaaacacct gcaagtgctg gcattggaat ctgtgacatc actggcgcaa aaacaagcat
     1381 ggagagcagt aagacaactg catgtatcat accagctaat ctagtttgtg caccagattt
     1441 gatggccacg cttgtacggg cgatggccgc agtagcagga atcccgccaa agaaaggaag
     1501 caacagattt ccaattcctt gcgccattaa ttcttgattg ctatctaagg gcttatttgc
     1561 cattcggcca gccgaagcac cacatagcaa gctttcaatc attcctaata aagcaatgct
     1621 aatagctgga actgccactt gttgcaaggc agaaaagcta aaatctccca tattcaaacg
     1681 attactactg atcaatgttt gtggaatctt acccacagtt gctactggta aatggaacaa
     1741 tagcataact gctgttgcca aaatgatagc cagcagcgaa ctaggcattc gtttcgccca
     1801 tttttttgga tagataaaca tccctaaaat gactagccct cccatgatca aagaggctgt
     1861 gtttatcgga aatcctatcg tttgataact acttagtttt tcaataacgt ttgtaccttc
     1921 cgagtgaaca ccaaataaat tatcgatctg acctaatgca ataatgatag caatacctga
     1981 agtaaacccc gtaattacgg gtgctgggat aaaagaagtc agtgttccta atcggaaaag
     2041 tcctgctgca agtaaaaata gacccgctaa aaaagtagca agcaatacac cttgcattcc
     2101 ctttgttgcc gcaatagaca tcaagatagc tgccatcgca cctgtcggac ctgatatttg
     2161 ataaaaaccg cctgataaac cgccaataac gataccggca atgatagccg taatcaaacc
     2221 tgcggccgca tcagctccgc ttgatacccc aaaagccaag gctaaaggca atgctacagc
     2281 agctactgtg atcccagcca aaagatcttt ctgaaatctt gaaagatcat aacctgaaaa
     2341 ttcttttttt agtgaggaaa cataattttt gaacacctat ctaactcctt tttcatgaaa
     2401 ttttctaaaa taccattcat ta
//
LOCUS       DACENJ010000256         2416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.264, whole genome shotgun sequence.
ACCESSION   DACENJ010000256 DACENJ010000000
VERSION     DACENJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.264"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..354)
                     /locus_tag="IW748_002500"
     CDS             complement(<1..354)
                     /locus_tag="IW748_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1105394.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLN"
     gene            complement(773..991)
                     /locus_tag="IW748_002501"
                     /pseudo
     CDS             complement(773..991)
                     /locus_tag="IW748_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1022..2236)
                     /locus_tag="IW748_002502"
     CDS             complement(1022..2236)
                     /locus_tag="IW748_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1105395.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT          754 a          535 c          386 g          741 t
ORIGIN      
        1 atttaaatct acttctgtga tatacatttt atctactatt tgcaaagctt ccttaaatag
       61 tccatatcca ccagatatgt atacatctcg tcctttagcc aataataatg catcttctaa
      121 tgatttaact gaaactaaat tatctccttg atactctgtt gtggtggaaa caacaatatt
      181 cattctatta ggcaacggat gaccgatttc ttcataagac tttcgcccca taataaccac
      241 attacccgtt gttaactctc taaattgctt ttgttctccc tttattttcc atggtatatt
      301 accattcttg cctataacat tattctttga cctcgcaaca atcaaaccta tcatctattt
      361 catcctccat aatatgatgt cgtacctgtg attccatcaa gtgcaccaca tcttaacaaa
      421 ctcaaaatta tctcgaacta accagctcga caaattcctt ttttgtcttc atctgcatta
      481 tatcatattg ttataagcat tgccatttct tttctgcttt tgaaaagtta cttatcagcc
      541 tatgttttgt aaagggtgat attcagcatt tctcacgcaa caagcccttg acaaaccata
      601 gactaataag ttttaaggta atcaagcaga aatgaaatag tctgctgttt cacggcaaca
      661 aaaaagagat accaacacac ttatgtagta tctcttagtc tagtagcaat attcttttgt
      721 ttgcaagcag gtaaacatgg gctgtttctt aatatctttt tgtttgggaa acaccttttt
      781 cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata aaccatagca
      841 aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat gtgggatatt
      901 catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaac gttaattttt
      961 ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc tcttttctat ttgaatttgt
     1021 ttcaattgag tttgttgcca aagaagaaaa agtccaatcc ttcttcttgt tcttcttttg
     1081 tagtagtgat cgttgtaaag tgcatcgccg attttaaaat cactgttgtc agcacatagg
     1141 catacacggc agtaatgatg acaccaatca cttgaatccc taagaagtac cagctgcttg
     1201 gattcgttag taaatggctg ttagatgcaa agatccctat cagaatcgta cctgtgaatc
     1261 cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc ccattgtttc tttttacgaa
     1321 aatcaacagc agcatgacag acgatacccg caatgatccc aatcagcatg gcactttttg
     1381 cttctacata accggcacaa ggcgtaatgg ctgccaatcc agcaacggat cctgttagta
     1441 cgtcgacaaa atcaaagcca tttcctttta cttttgcaat gatcagccaa gtaatcatcg
     1501 caaatgcaag agcgatgaat gttgaaccaa atgcattgac tgcttgttca tctgctcgaa
     1561 gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag cagtcctgta ccgatagcag
     1621 ctgccataag actgctatgc tgacttcttt ctcctaaagg aatctttctt tgaccaagtg
     1681 tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg gatgactgct ccaccagcga
     1741 aatctctaaa tcccatctga tccaaaaatc cgccacccca tatccagtgg caaacaggga
     1801 aataaatcag cagattccaa aaaataacaa gtataatata gccttttaaa ttcaatctcc
     1861 ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat aaacatcagt tggtaaagaa
     1921 agaacattaa aaatggaatc gttgcaccat gaaacatgtt ggggaagctt gtaacatgtc
     1981 tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc gatatcccgt ccaaaggcca
     2041 atccgaatcc accaaatacc cacataatcg ttgttatccc aacggagaga aaagattgag
     2101 acatgatcgt tagtatatta ttcttccgtg ctaatccgcc gtaaaaaaaa gctagaccag
     2161 gagtcatcag gcaaaccatt gctgtacata aaatcatgaa tgctgtatct cctgtattga
     2221 taggtaatgt attcattttt ttcgcctcct ttctacgatt acgagtataa aagaaggtgt
     2281 agttgttttt ttcttctgtt tttttgaatt aatacttgca tttataaaag acagacaatt
     2341 aataaaagct tgatgtgttg tgctcttttt cgtttttcac acaaataaag acaaaaaata
     2401 aacggtcttc ttttta
//
LOCUS       DACENJ010000257         2416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.265, whole genome shotgun sequence.
ACCESSION   DACENJ010000257 DACENJ010000000
VERSION     DACENJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.265"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1091)
                     /locus_tag="IW748_002503"
     CDS             complement(<1..1091)
                     /locus_tag="IW748_002503"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1105396.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGK"
     gene            complement(1203..1535)
                     /locus_tag="IW748_002504"
     CDS             complement(1203..1535)
                     /locus_tag="IW748_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1105397.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1683..>2416
                     /locus_tag="IW748_002505"
     CDS             1683..>2416
                     /locus_tag="IW748_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1105398.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVV"
BASE COUNT          649 a          509 c          441 g          817 t
ORIGIN      
        1 ttttttccag tttcttctag caagatttta ccatctactg ctaattcgct taatgacttg
       61 ataggtaaac gatcatagga ttctcctacg gcatccatct gaggtgtttc gtcaaaatag
      121 atcatcaatt cttctacttg cagtgcatgt tttttgccta agaagtacaa ttcgaatggt
      181 tccaaagctc cagtttctcg ttgtcgtagt ccttggacaa gtgcttgaac gtctttaatc
      241 atttggttag aacacttcca cgatttcaaa aaggaacgaa tctcattttc ttttagatcc
      301 agttggctga tcaataatgc ccatgcttgg ctctcttctt tcaatgggac ggatggtaat
      361 tcagcaaaac gtaaaagtgc ttcgccttct tctttcaaac cgggacagca aatgtagcac
      421 tctgtttcaa taaatgcatg gattcccgcg gcccgttcat ttcccaagag catcttgatg
      481 aattccacat ttatacgttc tacagatatt ttacctaata gctcgtggtt ttctttgatg
      541 gcgtagaagg tttcttcctc taatttgaac cctagttgac tgacaaaacg caacccgcgc
      601 atcattcgta aggcatcttc atggaaccgc tcatgaggat tgcctacagc tcgcaagatc
      661 tgttcttcta gatcttgcag accatcgaac aaatcgatga tttctccatc ttctttcaaa
      721 gcaaacgcat tgatcgtaaa atctcttctt ttcaaatctt ctgccaacga acgaacgaat
      781 tcgacatgat ctggtctacg gtagtcctga taagttgatt ccgtccgaaa tgtggtgatc
      841 tcatattgtt catcattttc taataccaac acagttccat gttcgatccc aatatctatc
      901 gtttttggaa aaagctgttt gatttcttct ggaaatgcgc ttgttgcgat atccacatca
      961 tggattggct ggttcagcaa gatatcacga acacttccac ctacaaaata agcttcatat
     1021 cctgcattct ctaatttttt cagaactgga attgcctttt ggtattccag aggtaattgt
     1081 tttaatttca tttttccaac ctttcctgcg tgtttcttgt cctgagataa agactcagtt
     1141 gcatggtgac tgtttgacaa gtccgtttca gagtcttcaa ggccaggacc acttcatctt
     1201 gtttactcgt ccttacgctc aaagcgatta tggtctcttt gattgaattt ttccatgttt
     1261 ttttcaaaaa cttcagttaa gtcgatattc aatgaattag ccatgatcat cgtaacgaat
     1321 aaaacatcac caagttcttc tgctaccgag ttaactggtt cagaagcctt tttgcttttt
     1381 tctccataag tgtggttgac ttctcttgcc aattcaccta cttcttcagt taatcgagcc
     1441 atctgagcta atggagagaa ataccccacc ttgaattgct ggatatattc atccacttcc
     1501 tgctgcatac tgtataaaga acgcttttta tccatctttt tccctccaaa actatcttac
     1561 caaaacttta acgcaaatcc tagataagag cacttgagaa aatcgaattt ccatgctaca
     1621 tttaggaagg aatttctaaa tggaaattct gtgaaaatac ataattgaaa gctggaattc
     1681 atatggtcaa acgaaaaaca gtaaaagaag ttctttttat cattatagga acaagtattt
     1741 atgcatttgg tcttgtttac ttgaacatcg ctaatcattt agcagaaggg ggagtctcag
     1801 ggatcacctt gatcttaaga gctttatttg gtattgatcc agcttatacg actttattaa
     1861 tcaacatccc attgatttta ctaggaggaa agatcctcgg taaacgttcc ttggcctata
     1921 ctgttctagg tacactttct ttgtcattct ttttatggat ctggcaaagg gttccactag
     1981 aaatcaattt acagcatgat ctactgatcg tttctctact tgcgggttta gttgcaggtg
     2041 taggaagcgg gatcgtctac cgcatgggtg gaacaacagg cggcagtgat attatcgctc
     2101 ggattttaga acaaaattac ggcatcacta tggggcgttc cctattggca tttgatatca
     2161 tcgtcttact tgcttcatta acctatatcg acttgaaacg tatgatgtat acgttgattg
     2221 tttcgtatgt tttcagtcgt gtgattgatt cgatcttaga tggcggatat tctgcaaaag
     2281 gtctacttgt cgtctccaac aaaagcgaag agattgcacc actactgatg accggtctgc
     2341 aaagaggtgt gactttcctt tctggagaag gcgcattctc tggtaattca aaaaaaatca
     2401 tctatatggt cgttag
//
LOCUS       DACENJ010000258         2393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.267, whole genome shotgun sequence.
ACCESSION   DACENJ010000258 DACENJ010000000
VERSION     DACENJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.267"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..151
                     /locus_tag="IW748_002506"
     CDS             <1..151
                     /locus_tag="IW748_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1105399.1"
                     /translation="EKAKRLVLENELTYTEIADILGFHSIHHFSSAFKKYTGLSPSKY
                     YLSLK"
     gene            292..1905
                     /locus_tag="IW748_002507"
     CDS             292..1905
                     /locus_tag="IW748_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1105400.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          761 a          427 c          461 g          744 t
ORIGIN      
        1 ggaaaaagca aaacgtctcg tgctagaaaa tgaattaacc tatacagaaa ttgcagatat
       61 attaggattt cattcgatcc atcatttttc ttcagctttt aaaaaatata cagggttatc
      121 cccttcaaag tattatctat ccttaaaata gaaaaaaatt tttaggtttt atccacaaat
      181 tatttctcta tattttctgt ctattcaaaa taaacaataa ttttccggca caattatttg
      241 ctgtttttta ccataacata gtacgatcaa cgtagaaaag gagatgtttt tatgaaagtt
      301 gttgtcattg gcggtgtagc tggcggtcct tcatttgcca ctcgtttccg tcgattgaat
      361 gaagcgcacg aaatcattat ctatgaacgc ggagaaaata tttcttatgc aagttgtgct
      421 ttgccttatt atttaggagg tgtgatcact gaccgtgact cgctgatcga acgtacacca
      481 gaaatattga aaacaaaaaa caacatcgat gtctttacta aacacgaagt aacagcaatc
      541 gatccttcta ctaagcgatt aacagttaaa gacctatcca caaatgaaga aataaaaaca
      601 gattacgata agttgatcat ctcttctggt gctagaccag attatccgga tattcccgga
      661 gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga cggatgcgga tcgaatcaaa
      721 tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc ttggtgcagg tgttatgggt
      781 ctggaattag ctgagaattt caagcatcgc ggcttagacg tgactttaat cgatcaattg
      841 ccacaagtcg ctttccctta tgatccagaa attgctaatt tagtttatga caaattgctg
      901 aaagaaggat tagccgttca tttagaaaca agagttactg agatccgtga taaaggtcga
      961 gaaatagtat tatcagatgg ctccgtcctt tctgctgata tgctaatttt tgctgttggt
     1021 gtttctccga agaatgaagt ggtgaaagca accggcatac aattatcaga tacaggtcag
     1081 atcattgttg atgaccagtt acaaaccaat ctgccggaca tctatgcgat tggcgatatt
     1141 atcgaaacga ctagtgtagt gactggtcag ccgatccaaa gtatgctttc cagtgcggcc
     1201 aatcgtcaag gacatatgct ggcagatatt ttaaatggta cgcctatgcg ctatcgcgga
     1261 tatattggtg caggtgtcgc aaaaatcttt gatcatacag caagttatgc gggaatgaca
     1321 gaacatgaat taaaagcatc aggcataaca aattataaaa ctgtttttat cactcccttt
     1381 gaccatgcct atttctatcc agaagctaca agattaaatc taaagctgat ttttgatgca
     1441 gatagcggtc gtattttagg tggacaagca tttggagaaa aaggtgtcga taaacggatg
     1501 ggagaacttt ctgtagcaat caccggaaac ttgacagtct ttgatttgcc tgatttggag
     1561 ttgccttact ctccaccgta ttctactacc cgtgacccgt tgaatatagc tggttatgtc
     1621 gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag caagtgatat acccgaaaac
     1681 gatttgaaag aagcgttctt tttagatata cgtgaaccta ataaagcacc ttctggcagt
     1741 atctcggcaa caaaaaatat tccaatgaat gagttacgag atcgaatcaa tgaaatccca
     1801 aaagataaaa aagtttacat tactttcaga agaggattga atacttatac ttctgcccga
     1861 atcttggcag gtttgggtat caaagcgatt ttgattgaag aataaaaaaa cgaccgcggt
     1921 gggtagccgc ggtctaggag ttatttttac ttgaggagta aaaatgaaaa agtatatagg
     1981 gttgttggtt ggtatgtata tagaatagaa gataaatgtg aagaaattgt gcattatttc
     2041 ttcacattta ttaggttata gtttaagaaa gtttaaacat tgcgcaattt aattatttta
     2101 gctgtttctt ttcataaaaa aacatgttgt gactctgaga caaatcatag tccatagtga
     2161 tgtctctaga gtaactacgc ctaatattac gaggcatcat attaatctat cagcactagt
     2221 acctaaacta gcatcccttt cgaatatttt ttcttatata ataaagtttg ctttcactcc
     2281 tctaatctca ccaaatcgac tggcttgcgt tgtgtgatac taggaactga ttggtttaga
     2341 acgacttttc tattgtaatc atatttttcg ccatcctcgt tgtcttctaa tcc
//
LOCUS       DACENJ010000259         2370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.268, whole genome shotgun sequence.
ACCESSION   DACENJ010000259 DACENJ010000000
VERSION     DACENJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.268"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(121..687)
                     /locus_tag="IW748_002508"
     CDS             complement(121..687)
                     /locus_tag="IW748_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1105401.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(687..1643)
                     /locus_tag="IW748_002509"
     CDS             complement(687..1643)
                     /locus_tag="IW748_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1105402.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1643..2299)
                     /locus_tag="IW748_002510"
     CDS             complement(1643..2299)
                     /locus_tag="IW748_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1105403.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          707 a          502 c          444 g          717 t
ORIGIN      
        1 ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga aatcaacgct
       61 ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg cgttttttat
      121 ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt tgataaaccg
      181 ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat caaccatatc
      241 cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc gtccgcgagg
      301 cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca tagtgatgat
      361 tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga gattttgttg
      421 ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga cacgttcttc
      481 taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct ggatatcaaa
      541 actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat cgtggccatt
      601 gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca gtaatgtatg
      661 gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac atcatatttt
      721 ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac ttcccatttt
      781 ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt caaacgatgg
      841 atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac atagtcagga
      901 cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt tttgcgcatc
      961 agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt gcgtctgaca
     1021 ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg cgtacaaaca
     1081 cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata atcatgtgct
     1141 cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc tacccataaa
     1201 tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag acaatccggt
     1261 cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc aaatcgttct
     1321 ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac gatgtattgt
     1381 tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg gattggaaga
     1441 gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa agtacaaccg
     1501 ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat cggcaccttg
     1561 aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata tcttttattt
     1621 ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta tctatttgtt
     1681 ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg agtaagtagc
     1741 cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc ccgacactta
     1801 ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt tgaacaaaag
     1861 ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca tgtccactag
     1921 ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct cgcatgaaaa
     1981 atagtttgat caccgggttg atgattccag caattacaat aaatccagaa acgaaccaaa
     2041 atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca aataaaatga
     2101 tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc agatcaggat
     2161 aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac gacagatgga
     2221 actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca gccacctgaa
     2281 atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta tagcattttt
     2341 ggtccagcga aaaaaacggt aaagagataa
//
LOCUS       DACENJ010000260         2341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.269, whole genome shotgun sequence.
ACCESSION   DACENJ010000260 DACENJ010000000
VERSION     DACENJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.269"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..617)
                     /locus_tag="IW748_002511"
     CDS             complement(<1..617)
                     /locus_tag="IW748_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ1105404.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KLITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVE"
     gene            complement(640..>2341)
                     /locus_tag="IW748_002512"
     CDS             complement(640..>2341)
                     /locus_tag="IW748_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ1105405.1"
                     /translation="EETIDSMTLEEKIGQLFVNMGSSRDKNYLSNMVNQYHIGAVRYN
                     PGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGTEVGVEVKIGATNDAKYAY
                     ELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQNPDKVLELSLAYMKGIQ
                     ESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWDNSFGKVYSGLIEAGLPSIMAGH
                     IHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGKLGFNGLVVTDASHMLGMTGAMK
                     RSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKGIISDDRLLDALKRILGLKAKLG
                     LHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQAITLVKDKQDIWPIEPEKYKRIL
                     LVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVEKYISPEEELKGLSQEEKIKN
                     IAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIVWPASKGTPDIPFYIHEIPTIFV
                     SVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLSEFKGVSPIDAYCGLKDTFI
                     "
BASE COUNT          734 a          476 c          295 g          836 t
ORIGIN      
        1 aattctacac ttgcatccaa atcagttatt cttgtattaa tagtaatatc acttatatag
       61 tctttcggag ttgtatataa cttaacaggt ctatgaatac ctgcataatt gaagaaatca
      121 aaattaacta agttttcctt ttttatatcc ccattttcct gtactgtttc cttcataatc
      181 cccacaggta aagtactatc gtcaataata ttgtttacag caactgtaat tcgattattc
      241 ccgtctttta caaaccgatt aatttcagac tcaaatggtg taaacccacc tttgtgttga
      301 gtaatcaact ttccattcaa aaaaactttt gcttcatgtg ttgcagatcc aaaacgaaga
      361 actaatcttt cttctaataa tcttttagga agagtcactt ctttttcata ccacacccat
      421 ccaacatgat ttctaatgtt ctctgtttca aataaatcat tataagaaga gggaactgaa
      481 atcggataac tctctttttc atttattggt tctaaagcaa gttcttcctg taagtcatca
      541 tttgtatcta atttaaatct ccagagaccg cttaagtcaa ttacttgcct cgtctctgtc
      601 ataatgggat acaacatata aaccactcct atctaaatat taaataaatg tatcctttaa
      661 accacaataa gcatctattg gacttactcc cttaaattca gaaagaccca ttagtttgtc
      721 caccaatatt tctaaagttt cttgttttcc atcataagta ttaatataag tctttacttg
      781 aggtacatca gccaaatgaa acggacactg tacagataca aaaattgttg gaatttcatg
      841 aatgtaaaac ggtatatccg gagttccttt acttgctggc cagacaattc gttgaactgt
      901 gcctggatta acattagcta tattaatgat caagtcatat tttttagtta aatttttaat
      961 tggtctctta gcagcatata ttccagctat atttttaatt ttttcttctt gcgataatcc
     1021 tttcaactct tcttcaggtg aaatgtattt ttcaacttca aatccttttg ctgacaaaat
     1081 ctgtttcaat aaatcaatgg cttgaggtct atttccagca actacttcac caaacccacc
     1141 ttttacccca tgtacatcta ctagtaaaat ccttttatat ttttctggtt caataggcca
     1201 aatatcttgt ttatctttaa ctaatgtaat tgcttggtca gccacctctt taaaaattga
     1261 tttattttca attaaaccaa ttttttccag agcttcttgc ttaggtaata aaatttgttc
     1321 ttttgctttt ttgtgtaatc ccaatttagc ttttaaccct aaaattcttt ttaaggcatc
     1381 taataaccga tcatcagaga taattccttt acgataacct tccatcatat aattaaagtc
     1441 ttcatctgga tcattaaaaa atagaaatag atcacatcca gctgcaatag aagtgggcaa
     1501 taattctgat cgtttcattg ccccagtcat tcctaacata tgactagcat cagtaactac
     1561 taatccatta aatccgagtt ttcctctaag taagtcagtg attaattctt ttgataaagt
     1621 tgcaggaagt aaatcttcat ctttcagatc aggattaaaa tgttttgaat aagctggcat
     1681 atggatatga cctgccataa tcgaaggtaa tccagcttct attaaacctg agtatacttt
     1741 tccaaaagaa ttatcccatt cttccgtacc taaactatta atactaaaag atagatgctg
     1801 atctcgttcg tcaataccgt caccagggaa atgtttagct gcaggcaaaa ttccactctc
     1861 ctgaatacct ttcatataag ctaaactaag ttctaaaact ttatcagggt tttgtccaaa
     1921 agatcgacta gaaatgatag gatttctcca atttctatta atatctacaa taggagcaaa
     1981 actccagtta catccaatag ccgatgcttc aatcccagaa actctcccta attcataagc
     2041 atatttcgca tcatttgttg caccaatttt tacttctaca ccaacttcag taccatctgt
     2101 acaagctccg ttaccacctg cttccgtatt agcagcaatt aataaaggaa ttttgctttc
     2161 tgtttgtagg atatagtttt gatcatatac ctcatcagca gttcctgggt tatatcgtac
     2221 agctccaata tggtactgat tgaccatatt actcaagtaa tttttatcac gactagatcc
     2281 catattaaca aacaattgac ctattttttc ttccaaagtc atagaatcaa tcgtctcttc
     2341 a
//
LOCUS       DACENJ010000261         2290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.270, whole genome shotgun sequence.
ACCESSION   DACENJ010000261 DACENJ010000000
VERSION     DACENJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.270"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..150)
                     /locus_tag="IW748_002513"
     CDS             complement(<1..150)
                     /locus_tag="IW748_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 domain-containing protein"
                     /protein_id="HAQ1105406.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTY"
     gene            373..1125
                     /locus_tag="IW748_002514"
     CDS             373..1125
                     /locus_tag="IW748_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1105407.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1265..2188)
                     /locus_tag="IW748_002515"
     CDS             complement(1265..2188)
                     /locus_tag="IW748_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1105408.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
BASE COUNT          772 a          441 c          389 g          688 t
ORIGIN      
        1 atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat
       61 agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt
      121 actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta
      181 gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa
      241 cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa tttcgtgaga
      301 aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca
      361 ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg
      421 agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca
      481 ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga
      541 acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac
      601 cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc
      661 ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac
      721 aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga
      781 caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg
      841 aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat
      901 ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct
      961 cataagaagt ggttctctta cagctggcct gaatatcagc caaaagcgca taaaggacta
     1021 ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat
     1081 ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc
     1141 aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt
     1201 ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat
     1261 atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa
     1321 acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa
     1381 gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat
     1441 agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa
     1501 gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa
     1561 caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt
     1621 tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct
     1681 tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct
     1741 gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat
     1801 catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt
     1861 cttcaagata ccttcgctct ttttctttat gatagcccgt cggtaaaagt gtcactttgt
     1921 aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat
     1981 tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat
     2041 ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct
     2101 catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga
     2161 gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc
     2221 ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg
     2281 cgaaaaactg
//
LOCUS       DACENJ010000262         2287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.271, whole genome shotgun sequence.
ACCESSION   DACENJ010000262 DACENJ010000000
VERSION     DACENJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.271"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            20..2038
                     /locus_tag="IW748_002516"
     CDS             20..2038
                     /locus_tag="IW748_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1105409.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
BASE COUNT          775 a          451 c          486 g          575 t
ORIGIN      
        1 tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa gaacggagac gtttagaaca
       61 gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg cgatggtagg
      121 ctgttctgtc gctgccccat tagtacagcc ggttcctgtt caagcggatg aaacacctgt
      181 tcagttcggc gcaagaatca atacagcggc atttatcaca gaaattgcta cttatgctca
      241 accaatcgct caagccaacg atctatatgc atcagtgatg atcgcacaag cagttgtaga
      301 aagcggttgg ggcagtagtg cgttatctca agcaccttat tataatcttt ttgggataaa
      361 aggcagctat caaggacaaa ccgtctacat ggatacgctt gaatttttaa ataatcaatg
      421 ggtaaataaa aaggagcctt tccgacaata tccttctttt gctgaatctt tcaatgacaa
      481 tgcctatgta cttagaaata cttcatttgg caacggttat tactatgccg gtacttggaa
      541 gagcaacacg aaatcctata cagatgcgac agcttgtttg acagggcgtt atgcgactga
      601 tcctggttac gcaggcaaat taaataatat catcgcaact tacggattga cccaatatga
      661 tacaccagct agcggaaacg caggcggtgg agcgactaca ggtggcaata atactggaag
      721 tgcaggtagt actggcggtt caagcaattc tggtaataca aactcaggtt cagcaacttc
      781 gaccacttat actgtccaat ctggtgattc tgtttgggga atctcaaata aattcggtat
      841 cacaatggat caattgatcc aatggaacaa catccaaaac aatttcattt atccaggaca
      901 aaaactgaca atcaaaggta gccaggaaaa tggctcttcg acgaataatt ccggtaacaa
      961 taccaactct tcaggaaatg ctggtacatc aaacggagga caaacaactg gtgcgaaata
     1021 cactgttcaa tctggtgatt ctgtttggaa gatctcaaat gatcacggaa ttacgatggc
     1081 tcaattgatt gaatggaaca atatcaagaa caatttcgtt tatccaggac aacagctaat
     1141 cgtatcaaat ggcggttctt ctaatgctgg agcaaatact ggctcaaata ctgggaatac
     1201 aagtggctct agttcgtcca gctcaagttc ttctaataca gcttcaggaa cgaaatatac
     1261 agttaaagcc ggagactctg tttggagcgt ctcaaataaa tacggaatta cgatggctca
     1321 gctaatccaa tggaacaata tccaaaataa ctttatctac ccaggacaac aactgactgt
     1381 ttcaaacagt ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa ccaatacaaa
     1441 taacagcagt tcagttaata ctggcagcag ttcgtctcaa ggcacttata ccgtgaaagc
     1501 aggtgaatct gtttggagcg tggcaaataa aaacggcatc acgatggatc agttaatcga
     1561 atggaataat atcaaaaaca acttcattta tccaggacag caattgatcg ttaaaggtgg
     1621 aacttcggca aatacaaata caggttcaac tacttctgca gcgaaaccaa atacaccgaa
     1681 tacgtctgct acgacaagta catcatccac tggtaatacg atgtataccg tgaaagcggg
     1741 cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg atggatcagt taatcgaatg
     1801 gaataatatc aaaaataatt tcatctatcc aggacagcaa gtgattgtga agaaaggttc
     1861 atctcaatca acaaaccaac aatcagttac tggttcgaaa acctatactg tgaaatctgg
     1921 agaatctgtt tggagtgttg ctgacagtca tggaattaca atggctcaac taatcgaatg
     1981 gaacaatatt aagaacaact ttatttatcc aggtcaaacg ttgattgtag ctaaataaaa
     2041 tagctgttcg aagaaataac gattgaattc cacaaaaata tttgttacta taaggctact
     2101 taaatatgac aacacttgaa aaggaagtag taaaaagact gaagtttcag agagcgcatg
     2161 gctggtgaga atgtgcacgg atgcttttga actcgccttt gagttgccct tgctgaatac
     2221 ccgagtaagc aagcggcggt gacggccgtt acaacgttcg aggccttttc actaaggtaa
     2281 aattagg
//
LOCUS       DACENJ010000263         2284 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.272, whole genome shotgun sequence.
ACCESSION   DACENJ010000263 DACENJ010000000
VERSION     DACENJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.272"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1348..1866)
                     /locus_tag="IW748_002517"
     CDS             complement(1348..1866)
                     /locus_tag="IW748_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1105410.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          750 a          423 c          312 g          799 t
ORIGIN      
        1 tcagattaac gggtaggctt actgtctttt ttggcaataa ttttgcaatc aagccattta
       61 cagatgtaat gcgcggatac ttctgctaag attgcaaaaa acatatcgaa aaggtaattc
      121 ataatacaac ctcctcccct tgccataata ggccggggta gcagacagca taaatattat
      181 accataactg aaaatttcat tttagtacat ttcaatatat tgatgaacca gcacaataaa
      241 acatgccatg actaacagaa aaaaacttat taaaccagat ataacttgta aacatccaac
      301 tatatagaaa acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc
      361 atatttttac tataccctat acttttactt ggtatattta tgaataatcg caaatttacc
      421 cctatactac tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat
      481 taaatacacc tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa
      541 cagctgatag taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg
      601 aacttgcttc tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcggtact
      661 gggattaaac taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg
      721 atttatttat tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat
      781 ttatttaaga tctcttcagt atcagtaata ggataggcga taggccacat accctttgat
      841 ggttagaata aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc
      901 tatctccaaa ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa
      961 cccaaagtca gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat
     1021 tccatattta aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa
     1081 aaaatctttc ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc
     1141 attttatcga agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg
     1201 atgcaccctc tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag
     1261 ttagcaaaga acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc
     1321 cacaattgct tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt
     1381 tgttctaaaa gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca
     1441 tacagccgtt ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc
     1501 atcactcatt gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc
     1561 taattctttc aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa
     1621 ctgattccat ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt
     1681 ttcccatttt agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt
     1741 tctatccact tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa
     1801 aatatattta gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt
     1861 tctcattata ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat
     1921 ccacgatatt ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat
     1981 atgtgaatga tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa
     2041 ggagctaaaa aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat
     2101 tttcttcgta aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact
     2161 ttaagcgtta agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta
     2221 aacttcacga aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc
     2281 ttcg
//
LOCUS       DACENJ010000264         2261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.273, whole genome shotgun sequence.
ACCESSION   DACENJ010000264 DACENJ010000000
VERSION     DACENJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.273"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            105..380
                     /locus_tag="IW748_002518"
     CDS             105..380
                     /locus_tag="IW748_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105411.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            393..>2261
                     /locus_tag="IW748_002519"
     CDS             393..>2261
                     /locus_tag="IW748_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1105412.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSL"
BASE COUNT          667 a          360 c          503 g          731 t
ORIGIN      
        1 atggaaattt ctaactagca ataaacgatg aaacaatgaa atatttttca tccaaaaaga
       61 actttgctat aatgatggaa gtttgtgtat aggagatctg gaaaatgaga gagagttttg
      121 aacaacaaaa aaaactgttg catgatcgct atggcgccct ttcgatggat gaccgccgtc
      181 aaatactttg taagttgcgt aaaagaaata tattgatgta tcgtcagttg gaacgtctga
      241 aacatgatct gcttcgtttg gagtcaaaac gtgtacagtg tgaattagag gggaaccaaa
      301 cacaggttga agtagtcgaa accaaaattt taaagaaaaa agaacaattt ttaaaaatgc
      361 ttacacaaaa taaaaaataa aggggatgga cgatggaatt tgctgagtta gttattgtat
      421 ttgcagtaac gatcacctta tcgaacatag ctagccgcat tttgccaatg attccagctc
      481 cactgatcca aattttcctt ggagtggtat taggtctgac agagtgggga caatcaatcg
      541 attttgaacc tgaattgttc ttagttatga ttattgcacc attgttattt agagaaggag
      601 aaaaagcaga tatttcttcg attctcaaaa atttcgggac aattttgttc ttggcatttg
      661 gaggagtcat tttgaccctt gtcggggtag gtgcgacgtt atcattcctg ttgccaagtg
      721 tcccacttgc tgcctgcttt gcttttggag cagcgttagg tccgacagat gcggtagcag
      781 tcagttctct ttctggacga gtgaatatcc ctaaaaaggc gatgcatatt ttggaaggag
      841 aaggtctttt gaatgatgca tctggtgtta ctgccttcca gtttgctctt ggtgcgctga
      901 tcaccggatc tttttcagca gtaaacgcgg ggatgagtct agtagtttct agtattgggg
      961 gagctttgat cggctttttg cttgtctggt tcaaacaaaa aataattcat ttgatcgaaa
     1021 aagcatcagc acaagatgtc acagggtatc tgctgatcga acttttgctt ccttttctag
     1081 cctatgtatt ggcagaggca tttgatgtat ctggaatcat tgctgcagtg gcagcaggta
     1141 ttctccaagc ttctggtttt aggaaaatct ctgtgtttga tgcagaattg tcgagcttgt
     1201 cccacagtac atggacaact attgctttta cactgaatgc attagtattt atatttttag
     1261 gaatcgaatt gacgcaagtg ttctcgccag tatggggaga cggtttatat ccaaacggtc
     1321 ttttattagc gattatcgta ttaatctctg tcatgctttt tgtgatcaga ttcatttcta
     1381 tcagtctttt ttatgtattc aaagatggca gtaaaaagtt caaaaaacag ttgaacgaga
     1441 tcttgatctt aacatttggc ggtgtaaagg gaacagtcag tttggctacg atttttattt
     1501 tgccattttc aatcaataat atgatgtttt accagcggtc tttgcttttg tttctgacgg
     1561 cgggtgtcat cttagtgact ttggtcatag ggatcattgt cttgcctatg ctgacggaaa
     1621 cagaggaagc gaaaagcacg gatttgaatg ctttgatgat tttagaagag gtcgttgaag
     1681 ttttgcgaaa agaaataaag gaaattgatc gtaatacaaa agaattttta gccactgaag
     1741 cagtgataga aaattatcaa gaacggatac gtgatctgta cttggaagat ttaaccgatg
     1801 atgaaaagca agaagtacaa gaaatacaag cactgatttt gtcgatcgaa cgggatggat
     1861 tggatgaaag ttatcgttcg ggcaagcttt cttctaatgg ttatcgtttt tactcccgtt
     1921 ttctttctcg attcgaacat tctatcacaa gccagatttt gtcttttatc ggcttttggt
     1981 tcattgttgt ccgtaggtta atgcgaatcg ttctccatcc gaaaatgttt tggcaaagaa
     2041 gatatgctga tcgtcagaca tttattagtg atgaagatat ccaagaaatc agagagacat
     2101 atcaaaaaaa tactcagttg attattgaaa gtttagataa cttgactgat atttatgatg
     2161 aaacgatggt tcaatttttt atccagcaac gcaaacttga aggaataaaa atgaactcgg
     2221 ggaatttgat ttcttcgtgg atgatccagc aagatagtct g
//
LOCUS       DACENJ010000265         2245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.276, whole genome shotgun sequence.
ACCESSION   DACENJ010000265 DACENJ010000000
VERSION     DACENJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.276"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..335)
                     /locus_tag="IW748_002520"
     CDS             complement(<1..335)
                     /locus_tag="IW748_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1105413.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIW"
     gene            417..1592
                     /locus_tag="IW748_002521"
     CDS             417..1592
                     /locus_tag="IW748_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1105414.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(1669..>2245)
                     /locus_tag="IW748_002522"
     CDS             complement(1669..>2245)
                     /locus_tag="IW748_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1105415.1"
                     /translation="PQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELSKK
                     GYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMANSG
                     PNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVLEG
                     MDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          691 a          427 c          414 g          713 t
ORIGIN      
        1 atccaaatag gtaactgctc cgcaatagtg gtaaatccga tcttacgatt tttattcgtg
       61 aaataccttt ttctgcgata tggaacagct ggatttgttt tttctaacgt tcttaaagat
      121 aaactgattt ttttggaata ttcatcaatg tcgatgatct gaactgttac tggctgtccg
      181 acttttaaca atgaatggat atttttcgta taaccggatt gtacttctga aacatgaata
      241 agtccttgcg tctcgttatc aagagaaacg aatgcgccat aaggctgtat ccctgtcact
      301 tttccttcaa ttatttgtcc aattttataa gtcatctact tccctctctt cgcatttatc
      361 attatactgt aaatagctca aaaaaagaag cgacgaaaat gactggaggt cttaagatga
      421 tcgaatttga taaacagatt tcaagaaaag atacagaaag tgtaaaatgg gacgcaattg
      481 ctgagacata tcaaatggat gacttacttc ctttgtgggt agcagatatg gacttcttgt
      541 ctcccggtgg ggtagctaaa gcgtttagtg aatacatcaa acatggaatc tttggttatg
      601 ccactctgtc tgataaacta tatcaggcta tcattcattg ggaacgccaa caccatgctg
      661 tggagttgac gaaagaaaat atcgttttta caagtggtgt gttaaccagt ttagctgcag
      721 cagtacaaac ttttacaaaa ccaggcgatt ctattttgat tcatgaccca gtttatcctc
      781 cattttcctc tattatagaa acaaatcaac gtcacatcgt tcgaagctct ttgctagaaa
      841 aaaataatca tttcatcatg gacttgtctg acatggagaa aaaaatcgaa gaaaacaatg
      901 taaaagcaat gattttatgc aatcctcaca atcctggagg gcgtgtttgg agcaaagaag
      961 aattgaaaca gttaagcgaa ttgtgtttaa aacatcaagt gattctgttt agtgacgaga
     1021 tccatcaaga tttaacttta ttcgatcata catttacttc catgctaacg attgatccta
     1081 atttagatga tctactagtt gcttttactt ccgcaaccaa gactttcaat ctagctgcaa
     1141 tcaaaaattc aatggtattt atcaaaaatc cagagatgaa agctgatttt gaaaaacatt
     1201 tagtgatgaa ccaacagcac gaaatcaaca cttttggact gattggaacc caagcggctt
     1261 atgagacggg tggagaatgg ttaagccaac tgattcctta tctagaaaaa aatgttcaaa
     1321 cagttaatca gttttttgaa cagtatcttc cgaaagtgag aataatgaac cctgaaggaa
     1381 cttacttaat gtggttagat tttaccgcct atatttcaga cgaccaagaa ttggagaaca
     1441 ctcttgttcg gaaaggaaaa gtcgtattaa atcctggcat cacgtttggt ccttccggtc
     1501 atgggcatat gcgcttgaat attgcttgtc cagaagaaac tttactggaa ggacttaatc
     1561 gaatcaagta tgctttagag acaatcgatt aaatcattta ttcaaaataa aaggacgaga
     1621 ccttactagg tttcgtcctt tttcataatt actccgtacc ataccaaact attctgaaat
     1681 ttcaattgtt tcaatcacta catcatgtac aggtcggtct tgtggaccac gttgtacatt
     1741 tgcgatttca tctactgtgt ccatcccttc tagtacatgt ccaaagactg tatgtcggaa
     1801 atctagccaa ggagttccgc cacctttata cgcttcaatg atttctgatg ggaatccagc
     1861 accttctagt tgtccaagca tattagctgg cacatttgtg ttatttacaa taaagaattg
     1921 gcttccgttt gtgtttggac ctgaatttgc catagataaa gctccgcgaa ggttgaacag
     1981 ttcacgactg aattcatctt caaaactttc gccataaata ctttcgccac ccattcctgt
     2041 tccagttgga tcgccgcctt ggatcatgaa atcaggaata acgcggtgaa aaatcacgcc
     2101 atcataataa ccttttttgg aaagttcaat gaaattttga accgtcttcg gtgctagttc
     2161 agggaataat tgaacagtaa tgtcaccacg gtttgttttg ataacagctt ttggtccttt
     2221 ttcacttttt aaatctagtt gtggg
//
LOCUS       DACENJ010000266         2207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.277, whole genome shotgun sequence.
ACCESSION   DACENJ010000266 DACENJ010000000
VERSION     DACENJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.277"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            88..984
                     /locus_tag="IW748_002523"
     CDS             88..984
                     /locus_tag="IW748_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1105416.1"
                     /translation="MEINLFEAGNRIKQIREKHGYTMADLGKLVDANSPSTINNWEKG
                     NNLPNRKRLEKIALLGGTSVEWIKYGDFSDYVYRLTQNIEEKILKEYGETTLNNYREA
                     LLDELTIEKITYEEDTKILAAAEKINTQNIFQEVKYMYSPDEDYRNIEVKPDLGLTDD
                     NKNIKMIGQRIRKIRKKENITLEEFGKLFSPTADKAVISNWENGKNLPNSERIKKIAE
                     IGGVSELYLMTGVDSSVAESMYFLSEVALDALERLSIEEVNRIIESFAGYLNVISKID
                     DPDKREISLNAICQLSLLDKMG"
     gene            1033..>2207
                     /locus_tag="IW748_002524"
     CDS             1033..>2207
                     /locus_tag="IW748_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1105417.1"
                     /translation="MKPNLSLTSERIRKIIESKGLTLERFGDLIGDTPISSVNNWLRG
                     VSLPNKNRLLTIANIGNTSVDWIKWGTLEEYIASYLIDSGYELYIKDFPEIPYKVFKD
                     IQEKYSDTFSLDKDYELLNPIIKFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTT
                     LTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNVELKNLNIGNNIKNIRKSKN
                     MTQTDFAELMGLSRSYIGDLENNRSNPSIKTLEAIARALDVPIVALINDSSTDNIVEP
                     YESLTAENILLKQENEELKYEVESLRKRLYEIQQVVNQGAEKMIPNLKDVSKRIKYIR
                     KKLGMTMEQFGSHLSNSPKSTIATWESGRNIPSQKKLQLIAIVGETTTDWIKWGTLE"
BASE COUNT          857 a          314 c          410 g          626 t
ORIGIN      
        1 cctagttttt atgaatgacc gttttgtaaa tctgattgaa atagatttat atgatttcgg
       61 tatatgatat acattaggag gtgtcgaatg gaaataaatc tttttgaggc tggtaatcga
      121 ataaagcaaa tacgagaaaa acatggatat actatggcag acttaggtaa actggttgat
      181 gccaatagtc ctagcacaat taacaattgg gaaaagggaa ataacttacc taatagaaag
      241 cgtcttgaaa aaattgcact attaggtggt acttctgttg aatggataaa gtatggagat
      301 ttttccgatt atgtttatcg cttgacacaa aatatagagg aaaaaattct taaagagtac
      361 ggagagacta cactgaacaa ctaccgtgaa gctcttctag atgaacttac gattgaaaaa
      421 atcacatacg aagaagatac aaagatatta gcagctgccg aaaaaatcaa tacacaaaat
      481 atttttcagg aggtgaagta catgtattct cctgatgaag attatcgaaa tatagaagtc
      541 aaaccagatt taggactgac tgatgacaac aaaaatatta aaatgattgg acaacgtata
      601 aggaaaataa gaaaaaaaga gaatattaca ttagaagaat tcggcaaact attctctcct
      661 acagctgata aagctgttat aagcaactgg gaaaatggta aaaaccttcc taactcagaa
      721 agaattaaaa agattgcaga aattggcgga gtttctgaac tttatcttat gacgggtgtc
      781 gattcaagcg tagcagaatc aatgtatttt ctatctgaag tagcattgga tgctttggaa
      841 cgtctgtcaa ttgaggaagt caatagaata attgaaagct tcgccggtta tcttaatgtg
      901 atttcaaaaa ttgatgatcc agataagcga gaaatatctc tcaatgctat ttgtcaacta
      961 tcacttctcg ataaaatggg ttgattcaca aaaaatattg aaagattact ttactcacga
     1021 ggaggcttag ctatgaaacc taatctatcg ttaactagtg aacgtattag aaaaattatc
     1081 gaatccaaag gtctgacatt agaacgattc ggtgatttaa ttggcgacac accaataagt
     1141 tctgttaata attggcttag aggcgtgtct ttacccaata aaaataggct tttaacaatt
     1201 gcaaatattg gaaacactag tgtagactgg attaaatggg gaactttgga agaatacata
     1261 gcttcatacc ttatagattc aggttatgag ctatatatta aagactttcc agagatacct
     1321 tacaaagtct ttaaagacat tcaagaaaaa tatagtgata cgttttcact agataaagac
     1381 tatgagttat tgaatcctat aataaaattt actatagggg gcgttacttt gaatagaata
     1441 aaattttata ttgattccag taaaaagacc attaaacaaa ttagtacaga aactggtatt
     1501 aactatacaa ctcttacgaa ctattaccgt ggtgagcgaa atcctagaaa tactgaaaca
     1561 tggaatatcc ttgctgatta tttccatgtt cctgtaagtt atttgatggg attagacgaa
     1621 acaaatgttg aactcaaaaa ccttaatatt ggaaacaaca tcaaaaatat tagaaaaagt
     1681 aaaaatatga cacaaactga ttttgctgag ttaatgggtc tatcacgatc atatataggg
     1741 gatttagaaa acaacaggag taatcccagt ataaaaacac tggaagctat tgcaagagca
     1801 ttggacgttc cgatagtagc attaatcaac gattcttcta ctgataatat agtagagccg
     1861 tatgaaagtt tgacagccga aaatatcctt cttaaacaag aaaatgagga attaaaatat
     1921 gaggtagaaa gcttacgaaa aagattgtat gaaatccaac aagtagtaaa tcaaggagct
     1981 gaaaaaatga ttcccaatct aaaagacgta agtaaaagaa tcaaatatat cagaaaaaaa
     2041 ttaggtatga ctatggagca atttggttct catttaagca actcacctaa aagtacaatt
     2101 gctacttggg aaagtggtag aaatatacct tctcagaaaa agttacagct gattgctata
     2161 gtaggagaaa ccaccactga ctggattaaa tggggaactt tagaaga
//
LOCUS       DACENJ010000267         2204 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.278, whole genome shotgun sequence.
ACCESSION   DACENJ010000267 DACENJ010000000
VERSION     DACENJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.278"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(175..1101)
                     /gene="cysK"
                     /locus_tag="IW748_002525"
     CDS             complement(175..1101)
                     /gene="cysK"
                     /locus_tag="IW748_002525"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1105418.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1241..2128)
                     /locus_tag="IW748_002526"
     CDS             complement(1241..2128)
                     /locus_tag="IW748_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1105419.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          620 a          487 c          366 g          731 t
ORIGIN      
        1 taacatgaaa agaaaattca attttattgt attttaatga aaaaagacgt aaaacagtcg
       61 cgttcactat tctacgtctc tattcaaata atttttctca tcataaattt ttgtgtgttc
      121 ttttcatctt taaaagtttt ttacataaac ctatctggag aaaaaccaag tgccttatat
      181 ctcttggtaa agggctgtag ataaataacg ttcaccattg tcgggaacca cagctaatac
      241 cttcttgcct tttcctaatt cttttgcgac ttttagcgcg gcagcaattg cagcgccaga
      301 tgagatgcct actaaaatcc cttcttttcg cccaacttct ctagctgttt ccatcgcttc
      361 gtctccacta atagaaagga ctttatcata cacagaagta tccagtgttt taggaacaaa
      421 acctgtaccg attccttgga ttttgtgagg tcccgcttct ttcccttcta aaatagcaga
      481 ttctgctggt tctaccccga ttaattcgat tttcggatac acacgtttta attctcggcc
      541 agcgccagtg atcgttcctc ctgtaccgat acctgcaaca aacgcgtcca agccatttac
      601 tccaaatgct tgatgtatct caggaccagt cgtcttctca tgcacaagag gatttgcttc
      661 attttcaaat tgtaatggta aaaagtagcc gttttctttt gccaaacgtt ctgcttctct
      721 gattgatcca gaaataccat ccgcaccagg agtcaaaatc aactctgcgc cgtacccttt
      781 catcaataaa cgtctttcga tactcatcgt ctcaggcatc acgatgatta ctttatatcc
      841 ttttgcaact cctaccatcg acaatccgat tcctgtattt ccagaagtag gttcaatgat
      901 agtatcacct ggtttcagta gaccatcgtg ttctgctttt tctatcatac ttaaagcaat
      961 acgatctttt acacttcctc ctggattaaa aaactcaagc tttacaaaaa catctgctga
     1021 atcttctggt acgattttat ttaatttgac gattggcgtt ttgccaatca attcggtgac
     1081 tgagttatag atagctgtca tcttcaattc ctccttgtct ttgctcttct tacttatcat
     1141 aatacaatct gttcatttat ggcgagtgat tcgtctttga ctagcaaaaa aaagacaaag
     1201 atataccata tatggtaatt tctttgcctt tttatctaaa tcaaacgttt agttcttgac
     1261 caactaataa agaataactg gacatagaca tgccattttt cgccattaga tcatcaacag
     1321 acatgccaaa tttttgtgca atatcccata gcgtatcacc tgacacgact gtatatgtct
     1381 gaccattatt tgtatttaca tctgttgttg aagatgatga cgttgaagtc gtcgtagtag
     1441 tcgttgttgt tgtacttgtg gttgttgtag ccgaacctgt tgatggcgta tcaaaacggg
     1501 ttaaattata agtattaatc aatgagttca atttggcccc ataatttgga tcggtagcat
     1561 aacgacctgt caagtacgct gttgcatcat aaaaacttgt tgtattgctt ttccacacgc
     1621 cagcataatg ataatcgccc gtggaaaaac tggttgaacg aagaacattc gcatggtctt
     1681 ggaaggattc ccaataagaa gaatattttc ggaatggctc attcatcgtt acccattgtc
     1741 cgtttacata ttctttggtt gccatataaa cgacctgtcc gttatagctt cctttcacac
     1801 cgaatagatt ataatttggc gcactagcta atccggaagt gccatatgca ctttccaaca
     1861 acgcttgagc gatcatgact gaagcataca agtcattact tgcagcaact tctgacgcag
     1921 accagccgat ttgatcgata aacgcttgag gatcagcgac tgtttgagat ccttgttcat
     1981 ctgcatgaac atgttgcggt accgctgtaa ccatcatcgg cccaactaaa agactcgttc
     2041 caattaaagg agcaatcgct tttttattga tcaccatttt tcttttaggt ctatggtgcc
     2101 gggcactgcg ggtcttcagc tcttgcattc aaattcctcc tcaaaataaa aaaacttaat
     2161 aaaaagttct cttcttaaaa caaacttaat taattaaatt atac
//
LOCUS       DACENJ010000268         2179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.280, whole genome shotgun sequence.
ACCESSION   DACENJ010000268 DACENJ010000000
VERSION     DACENJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.280"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..228
                     /locus_tag="IW748_002527"
     CDS             <1..228
                     /locus_tag="IW748_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter solute-binding protein"
                     /protein_id="HAQ1105420.1"
                     /translation="QIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTNIPIYSTIF
                     TDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            243..476
                     /locus_tag="IW748_002528"
     CDS             243..476
                     /locus_tag="IW748_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1105421.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            807..1391
                     /locus_tag="IW748_002529"
     CDS             807..1391
                     /locus_tag="IW748_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1105422.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            1522..2179
                     /locus_tag="IW748_002530"
                     /pseudo
     CDS             1522..2179
                     /locus_tag="IW748_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
BASE COUNT          765 a          285 c          374 g          755 t
ORIGIN      
        1 caaataaaac acttagttga aaaattacgc acaacaaaag ttccctcctt attcgtagaa
       61 agcagtgtgg acgatagacc aatgaaaaca gtatcaaaag ataccaatat tcctatctat
      121 tcaacgattt ttactgattc aattgcagaa aaaggacaag atggtgatag ttactatgcg
      181 atgatgaaat ggaacctgga taaaattgct gaaggccttt cgaaataatt tggagggtag
      241 gtatggtaca aattaaaacg actactatac taatttattt actagcaatt caggaattat
      301 caaaaagaaa aaagaaatta aaaaataatg atttagcaaa agttctaagt gttagtccag
      361 catctgtaag tgagatgctg gctaaactta gatatgagga ctatttagat ataggatttc
      421 aattaactga taaagggaaa aaatttattg acgattacaa aaaaggtttt aagtaaaaga
      481 ctttattttt agggaggata agaaaatggc tattggagtg attatgatag gacttaccct
      541 aatagctgga tataattttt ttaaatcttt ctcggattat tttagaaaag gtgaatgatt
      601 tagatgagtc tattaattct tgtaggattg ctttttgtat cagttatttt gctctttctc
      661 attagtttag ctctaaggaa tgagttcagt aatagacgtt aacgattgaa acttcaagtc
      721 ctgtgtaaga taatatagtt aattcatctt attaggagga agtacccata gaaaccagag
      781 attatattac aactcaccaa aaattaatag aaagtggact aagccatttt gaaaaagaag
      841 gatttcagca aactaagata aggcaactgt gcttgaccgc aggcgtaact acgggtgctt
      901 tttataaaca ttttacttct aaggaagcgt tatttgaaga aattattcag ccgcatatta
      961 atgcgcttat taatatgtat aatgaagagt ttaccaaata cctcttaaac ttagaaaata
     1021 tagatataga gaaaagttgg agagagaact tcaatgaatt agatccattt attaattata
     1081 tttttgagaa taaacgtata ttcgatttgg ttttattcca atcagctgga accaaatatc
     1141 aagatattgt tgaaaaaatt acatcatttg tgacaaaaaa gacagtctgt tgtctagata
     1201 agttaaagac tatcggaatg atcaatcaaa atttagtctt taatgaagaa gaaatccact
     1261 tctacattta ctcattctac gctacattct atgatatatt gaagcatagc tattcaaaag
     1321 aaaagactat gctattaacc agaagattgt atcaattttc tgttcctggg tggattgaat
     1381 tactgagtta attaaaaaaa agcttgcatt gagaattgtt ctcagttatg ctttttgata
     1441 agatcgcact gttatctgaa taacggtgca tttttgtcag cttaagataa cagtgcgatc
     1501 ttattttttg gaggtgaaag agtgtcaaaa atcaatccgt taaccttgat tactttaaat
     1561 atttttttcc ggtcattatt tttttaggaa agggaaatat atttcaagtg gggtgtttca
     1621 ttattgctac agttttagta ttgatgtttc gtaaaataaa gacatgtatt tatttcatct
     1681 tgatttattc agttctgttt ttagtaatga aaattattga tgctatcaag atagagtttt
     1741 ttactatatt ttttgggaca tccttatata ttgtatttcg aatgttgccg gtcgtaatga
     1801 tctcatggtt gttggttagt gtctatacta gcaatgaatt attatcctct ttagaaaaaa
     1861 tacatttacc gaaaaaaata atgttaagta ttacagtagt gatcagattc tttccgactt
     1921 atcgttctga aataagaatg attaaagaat ctttaaaaat gagaaacatt cgcttgtctt
     1981 tttttcagcc tctgcaatat ttggaatttc tgttagttcc cgttcttata cgtgcaacta
     2041 tgattgctga agaaatgacc gctaatgcaa taacaaaggg gatagaatct cctgtgaaaa
     2101 gaagttctta ctataaaaca aacatgagct tattagacta tatcatcctg ttttccgtta
     2161 ctattttttt cttaagtat
//
LOCUS       DACENJ010000269         2177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.281, whole genome shotgun sequence.
ACCESSION   DACENJ010000269 DACENJ010000000
VERSION     DACENJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.281"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..950
                     /locus_tag="IW748_002531"
     CDS             <1..950
                     /locus_tag="IW748_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAQ1105423.1"
                     /translation="MNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSKGVI
                     LVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAIVT
                     DLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDVNKE
                     NILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIELGEK
                     IDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTA
                     DLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            947..>2177
                     /locus_tag="IW748_002532"
     CDS             947..>2177
                     /locus_tag="IW748_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1105424.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT          840 a          275 c          372 g          690 t
ORIGIN      
        1 ttatgaatcc agcaaataat ctgaactcat ttgctaaaga aaagttaaaa aagaacaatc
       61 taaatctaat aaacgcagta gagagatatt tagatgttaa aagaaatgga ttgctttttt
      121 ctaaaggtgt tatcttagtt gaaggagatg ctgaagaaat acttattcct caaatagtaa
      181 aaaaattatt tggtataaac ttagacgaac ttggaatagg tttgattaat gttggtagta
      241 cttcgtttga atatatcgct agcttgtttg acgatgatag aatacagaga aagtgtgcta
      301 ttgtaactga tttagatgaa caggcaatcc catcagaaca tcgattgtat aaaagcactg
      361 cacaagagaa aggtcaagca agaaaagaaa aattatcctc cctttttaat gaaaacaatt
      421 gggttaattg tttttatgct aatacaacat ttgaaattga gtttttagat gtgaataaag
      481 aaaatattct aacttatgtc agtccaatta tagatgacgc attcaaagat aaaaaaacaa
      541 taactgctta taaagaagaa atacaaagtg aacaatattt agaaagaaat ttagcgatgt
      601 taaaactagc caatcatgtt ggtaaggggt ggtttgcaat tgagttaggt gaaaaaattg
      661 actttttaat caaaattcct aagtatattc ttaatgctat tgcttttgca tgtcaggaaa
      721 tagtagatct ctcaatttat aaaaaaattc tactccatac aatcgacttg tacgaggaaa
      781 ctacagaatt gtcagcttta tcagctagtt taaaaacaag tgatacagct gatttgaata
      841 tagcaataat ttctttatta gagtactttg gtaaagatga ggatgttgtg tatctaatca
      901 atcagataga aaattacaca aaaatacttt atgctgaggt ttcaaaatga caaatatctt
      961 cgaaaaaatc gtaaatgatt taaatttaga acaaaaagat gttgtggaaa gtgaagataa
     1021 cctttatgta aatgcatgtc ctgggagtgg gaaaacaagg gtacttacaa ggaaaattgc
     1081 ttaccaagca attaaacacg atggatcact aaaaagaata attgctatca catacactaa
     1141 tagagctgct gaagaaatta aggaacgcct aagtttactt agtatagatg atgatattaa
     1201 tatttgggtg gggacaatac atcagttttg tcttgaattt attatttatc cttttaaaat
     1261 gaatctctca agagtatcaa aaggatttac aattattgat gattatactc aaagagctta
     1321 cgtcagccaa attaataata tgctgaattt aaatctcaaa acgtatgaac tgaacaaaat
     1381 tgatttgtca ctttctcatg attttaaaat taatgaagtt cagtatactc aagtttctga
     1441 aatgtatcac actatgttgg aaaaaaataa agaaattgat tttgatatga ttttactagt
     1501 agcgtataaa attttaactg taaacagtaa cgttgcacgt aatattgcta gtaatataag
     1561 ggcgatttat gtggatgaat ttcaagatac tagagaatta caatacaatg taatagctaa
     1621 attacttcaa aacaaccctc aaattcagtc aatgtttgtt ggagatattg accaagcaat
     1681 ttatggatca ttagatggta tagctaaatc agttgaagaa ctagaaatgc taactagtca
     1741 cacttttcaa tcaaagacgc tacatggctg ttacaggtct aatcagagat tagtagactt
     1801 ctactctaat tttcagagtt gtccttacga gattgaaagc agagggaata ataaaaacga
     1861 ccgtggtttt ataagttatg attgcaaagt tactaaggag gatttaccca acataataaa
     1921 aaatataata aaggaaaaga ttgaaagcgg cattcatgaa aaagatattt gtgtacttgc
     1981 tcctcaacac tatcctctgt tttcactggg tgagtattta aagaaagaat taccgtattg
     2041 caattttgat gctataaata tatcaccaat taaggtaaat aatcatagtt tatttttcaa
     2101 gttagcaatt ttgtacttta ctgaatcagg tgaaaaggtt cgaagaagaa agctaattgc
     2161 aaacgatatt ttaatta
//
LOCUS       DACENJ010000270         2175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.282, whole genome shotgun sequence.
ACCESSION   DACENJ010000270 DACENJ010000000
VERSION     DACENJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.282"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(86..1252)
                     /locus_tag="IW748_002533"
     CDS             complement(86..1252)
                     /locus_tag="IW748_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1105425.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTEFTVFDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFVILEILPKKIFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFALSMILLLSFLWLL
                     VRKCELTNGQLTFLWAFLAIMCQSFMEETLATISWVPIVFLMLAMATHQYKTTR"
     gene            complement(1258..1947)
                     /locus_tag="IW748_002534"
     CDS             complement(1258..1947)
                     /locus_tag="IW748_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ1105426.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDDVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
BASE COUNT          733 a          407 c          326 g          709 t
ORIGIN      
        1 tcatccatct gcctcctttc agacaaaaca aaaaacactt tttcacaacg aaaaagtgtt
       61 ttctcttatt actattctat ttcatttatc tggtggtttt atattggtgt gtagccattg
      121 ctaacattaa gaacacaatt ggtacccaac tgattgtagc taatgtttct tccatgaaag
      181 attgacacat gatcgctaaa aaagcccaca agaatgttag ttgtccattt gtcagctcac
      241 attttcttac gagcaaccat aagaaactaa gcaaaagaat catcgataga gcaaaaccaa
      301 tcaatccata gtgattgacg agtgcgtgat acgtattatg gttacctaga atctgatttc
      361 ctctatggaa aaagaaaggt ttcatcccta caaatatttg tcctgttttt tcgcctaatg
      421 tttcatagaa tttgcgccaa atttcttctc gtcctgtaaa taaatgaact ttgtctgaca
      481 ttgcagcaaa ataagaaatc ggtaaagcta gaaccgaaac tgctgcatac atccaaaagg
      541 acaagtgtgt gtttcgaaag atcttcttag gtaaaatttc taggataacg aagataacaa
      601 gcgcgtataa tgctgctttc gattggcaaa cccaaatcgt tgccccagca agaatcaacg
      661 ctggtaatga tagcaatcgc atgtaccatt ttccaaatga actagcaaat cctgtgataa
      721 gaagggctaa tgtcattaaa gaactaccta tcgtatttgt attgatccaa atatctgata
      781 atttgttcga tggattccaa atactttgtc cttctggtac tggtatctcg gtaaatactc
      841 gataaaccgt tacgagtaaa aagacataga atactacaag atacaagcta cgatcaaata
      901 cagtaaattc cgtccatttt acaatcaaaa taatggcaat aattgtcgga acgatcagat
      961 caccatagaa agtatctcct cggtagcttc taaaaaaaga aaagaaaagg taaaaaagaa
     1021 tgcctgctgc aatgaagaat agatcgtttc tctttacctt gtacagattc aaaagaaaca
     1081 aacagcctac aaaaaacgtc actaactgtg cagcattatc ataaataaat aatatcgtag
     1141 gaggatttaa ccccaccgtc catattaaat aaagaatgga taccaacagt aacagaaaaa
     1201 tggcactcat tttattgatc aatttttcta caagttcttg aatgtgactc acttccctta
     1261 tttattgatt ttttctacat tgactaattc gcactggatc acatcgcact ttttcatatt
     1321 atttgcttca aagtctaaca gtgtgagtag tggtttttta cctgcttttg gcaaggataa
     1381 aaaggaattg ttttccttag attgaactac ctggctgtaa tcataaattt ttcctctttt
     1441 ggagacttca tactgatact tgtaatgagc atcgattaga tcaatttctt ctccttcgga
     1501 taaatcaatc atatcggata ataaaacgct tggtgtatct acgttataac cagcaagaat
     1561 ataatttcca tttcccattt cagcatctgg ataataagta gaagctactc ctaaactaag
     1621 cgtgtcgcta ttcgctcctt gaaaaacagg gagaataaat cctgtagaag aaaaaacaag
     1681 ttttccagca atttcacctt tgagatcctt ttctaatgcc tgtttttgat tagtaatcag
     1741 ttgtctctta gaaatatccg ttaaactggt taaattatct tgataggttt tagcctgttc
     1801 ctgaatatgg attgtttttg ttacatcatc tactttttca gcgattttgt ccttttgata
     1861 accttgataa gcaaaataac taaaaaggag aaaaacaatg attacgccca cgtaaggatt
     1921 aaataaagtt ttattgtttt tattcatcta tctaccttct atatattaat taatttacct
     1981 acaactaacc aagataaacc acgttaaatt ttactattct ttttattcaa taacaatgcc
     2041 acatcataat tgatcatttt catatcataa tcgtcaagaa acttctaata atagttggca
     2101 attagaactt gttttcacat aagttcgtct caactgttca aaatttttca ttgatcccaa
     2161 tagacaggct ttctc
//
LOCUS       DACENJ010000271         2167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.283, whole genome shotgun sequence.
ACCESSION   DACENJ010000271 DACENJ010000000
VERSION     DACENJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.283"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..111
                     /locus_tag="IW748_002535"
     CDS             <1..111
                     /locus_tag="IW748_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1105427.1"
                     /translation="YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            325..1020
                     /gene="vanR"
                     /locus_tag="IW748_002536"
     CDS             325..1020
                     /gene="vanR"
                     /locus_tag="IW748_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1105428.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            998..2152
                     /locus_tag="IW748_002537"
     CDS             998..2152
                     /locus_tag="IW748_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1105429.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          731 a          400 c          461 g          575 t
ORIGIN      
        1 tatgcggtaa agctatataa agaaggaaat atgactgtaa atcaaatttg tgaaattact
       61 aatgtatcta gggcttcatt atacaggaaa ttatcagaag tgaataatta gccattctgt
      121 attccgctaa tgggcaatat ttttaaagaa gaaaaggaaa ctataaaata ttaacagcct
      181 cctagcgatg ccgaaaagcc ctttgataaa aaaagaatca tcatcttaag aaattcttag
      241 tcatttatta tgtaaatgct tataaattcg gccctataat ctgataaatt attaagggca
      301 aacttatgtg aaagggtgat aactatgagc gataaaatac ttattgtgga tgatgaacat
      361 gaaattgccg atttggttga attatactta aaaaacgaga attatacggt tttcaaatac
      421 tataccgcca aagaagcatt ggaatgtata gacaagtctg agattgacct tgccatattg
      481 gacatcatgc ttcccggcac aagcggcctt actatctgtc aaaaaataag ggacaagcac
      541 acctatccga ttatcatgct gaccgggaaa gatacagagg tagataaaat tacagggtta
      601 acaatcggcg cggatgatta tataacgaag ccctttcgcc cactggagtt aattgctcgg
      661 gtaaaggccc agttgcgccg atacaaaaaa ttcagtggag taaaggagca gaacgaaaat
      721 gttatcgtcc actccggcct tgtcattaat gttaacaccc atgagtgtta tctgaacgag
      781 aagcagttat cccttactcc caccgagttt tcaatactgc gaatcctctg tgaaaacaag
      841 gggaatgtgg ttagctccga gctgctattt catgagatat ggggcgacga atatttcagc
      901 aagagcaaca acaccatcac cgtgcatatc cggcatttgc gcgaaaaaat gaacgacacc
      961 attgataatc cgaaatatat aaaaacggta tggggggttg gttataaaat tgaaaaataa
     1021 aaaaaacgac tattccaaac tagaacgaaa actttacatg tatatcgttg caattgttgt
     1081 ggtagcaatt gtattcgtgt tgtatattcg ttcaatgatc cgagggaaac ttggggattg
     1141 gatcttaagt attttggaaa acaaatatga cttaaatcac ctggacgcga tgaaattata
     1201 tcaatattcc atacggaaca atatagatat ctttatttat gtggcgattg tcattagtat
     1261 tcttattcta tgtcgcgtca tgctttcaaa attcgcaaaa tactttgacg agataaatac
     1321 cggcattgat gtacttattc agaacgaaga taaacaaatt gagctttctg cggaaatgga
     1381 tgttatggaa caaaagctca acacattaaa acggactctg gaaaagcgag agcaggatgc
     1441 aaagctggcc gaacaaagaa aaaatgacgt tgttatgtac ttggcgcacg atattaaaac
     1501 gccccttaca tccattatcg gttatttgag cctgcttgac gaggctccag acatgccggt
     1561 agatcaaaag gcaaagtatg tgcatatcac gttggacaaa gcgtatcgac tcgaacagct
     1621 aatcgacgag ttttttgaga ttacacggta taacctacaa acgataacgc taacaaaaac
     1681 gcacatagac ctatactata tgctggtgca gatgaccgat gaattttatc ctcagctttc
     1741 cgcacatgga aaacaggcgg ttattcacgc ccccgaggat ctgaccgtgt ccggcgaccc
     1801 tgataaactc gcgagagtct ttaacaacat tttgaaaaac gccgctgcat acagtgagga
     1861 taacagcatc attgacatta ccgcgggcct ctccggggat gtggtgtcaa tcgaattcaa
     1921 gaacactgga agcatcccaa aagataagct agctgccata tttgaaaagt tctataggct
     1981 ggacaatgct cgttcttccg atacgggtgg cgcgggactt ggattggcga ttgcaaaaga
     2041 aattattgtt cagcatggag ggcagattta cgcggaaagc aatgataact atacgacgtt
     2101 tagggtagag cttccagcga tgccagactt ggttgataaa aggaggtcct aagagatgta
     2161 tataatt
//
LOCUS       DACENJ010000272         2157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.284, whole genome shotgun sequence.
ACCESSION   DACENJ010000272 DACENJ010000000
VERSION     DACENJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.284"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            87..1313
                     /locus_tag="IW748_002538"
     CDS             87..1313
                     /locus_tag="IW748_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1105430.1"
                     /translation="MRKDIYEGVLPFIMTHTKPNYAALAKQYNCDYRTVKRYYEAGIS
                     NKLPLLAQKNKNKKTLVSDFEDIIADKLALGCSSSAIYHFISKRGYRGSARTLRRYCR
                     NIKADKIQKATIRVETTPGLSAQVDWKEDLKLVNKFGEVISCNIFLYVLGYSRIKYLE
                     LTFDRSQTTLFRCLTNAFQYTGGVPTEIWFDNMKTVVDRSRSQFSKVIFNERFRQYAK
                     EAGFLPIACRAFRPQTKGKVEALARTIERIRVFNYEFESTHELEKIIQTIMDSLNTEE
                     ISQAIHECPSDRWSYEKEQLSSFDANQLKIYASVEKQIKRKVSNEAMVQWNQNKYSVP
                     VNYIGKILTLRVKEDKLYIYDDSTLIRTHTMTHQLLNYHRNDYIEILRSDVFKHLEDE
                     ELERFVDENLQAYDEL"
     gene            1328..2083
                     /locus_tag="IW748_002539"
     CDS             1328..2083
                     /locus_tag="IW748_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1105431.1"
                     /translation="MSNYYHLLNKLEDLNLKHTREILPEYLDTIVQENLSFVEALNHL
                     MTEEINSRNERQKENRLRKANLPFKKTINDFDFGFQPKINRTEILDLCTLRFMDTNNN
                     ILFIGNSGVGKTHLAIAITLEAITKGYSCHFALSNELVNRLNRANQRGNLDRLLKKYA
                     SYDLLIIDEIGYLPFTPDGAKLFFQLINLRYEKKSTIITTNIPLSQWAETFQDKKLTN
                     ALIDRLVHHAKIIQITGKSYRIKDYKEKKLSYK"
BASE COUNT          782 a          329 c          346 g          700 t
ORIGIN      
        1 gtagaaaaat ggccaaataa aaatgtaggt ttatgcgtca tctggtacgc tttccttgtg
       61 ttattttatt caaggaggaa agtgtattga gaaaagatat ttatgaagga gtgttacctt
      121 tcattatgac tcatacaaaa cctaattatg ctgctctagc taaacaatac aattgcgatt
      181 atcgtacagt aaaacgttat tatgaagctg gaataagcaa taaattacct ttacttgctc
      241 aaaagaataa aaacaaaaaa actttggtta gtgattttga agatattatc gctgacaaat
      301 tagctttagg ttgttcttct tctgctattt atcattttat ttctaaacgt ggctatcgtg
      361 gaagtgcacg aacactgaga agatattgtc gcaatatcaa agcagataaa atacaaaaag
      421 caacgattcg tgttgaaaca actcctggct tatctgctca agttgattgg aaagaagacc
      481 tcaaattagt caataaattc ggtgaagtta tttcttgtaa tatcttcctt tacgttttag
      541 gatattccag aattaagtat ttagaactaa cttttgatcg ttctcaaact actttatttc
      601 gttgcttaac taatgctttt caatacaccg gaggtgttcc tacagaaata tggtttgata
      661 atatgaaaac tgttgttgac cgtagtcgta gtcaattttc taaagttatt tttaatgagc
      721 ggttccgaca atacgcgaaa gaagcaggtt ttcttccaat tgcttgtcgt gcttttcgac
      781 ctcaaacaaa aggaaaagtt gaagctcttg cacgaacaat tgaacgaatt cgtgtattta
      841 attatgaatt tgaatctact cacgaattag aaaaaattat tcaaacaatc atggattcat
      901 taaatactga agaaatttct caagcaattc atgaatgtcc tagtgaccgt tggtcatacg
      961 agaaagaaca actatcttct tttgatgcga atcaacttaa aatttatgct tctgttgaaa
     1021 aacaaattaa acgaaaagta tctaacgaag cgatggttca atggaatcaa aataaatatt
     1081 ctgtgccagt aaattacatc ggtaaaattc ttacacttcg tgtaaaagaa gataaacttt
     1141 atatttacga tgactctact cttattcgca cacatacgat gactcatcaa ttgcttaatt
     1201 atcatcgaaa tgactatata gaaattctac gttctgacgt ttttaaacat ttagaagacg
     1261 aagaattaga acgttttgtt gatgaaaatt tacaagcgta tgacgaatta tagagaggtg
     1321 aatcattatg tctaattatt atcatttact aaacaaatta gaagatttga atttaaaaca
     1381 tacgagagaa attttaccag aatatctcga taccattgtt caagaaaatc ttagttttgt
     1441 agaagcttta aatcatttaa tgacggaaga aataaactca cgaaatgaac gacaaaaaga
     1501 aaatcgttta agaaaggcga atttaccgtt taaaaaaaca atcaatgact ttgattttgg
     1561 atttcaacca aagattaatc gaacagagat tttagatttg tgtaccttac gctttatgga
     1621 tacaaataat aatatattgt ttattggaaa tagcggagtg gggaaaactc acctagctat
     1681 tgccattaca ctagaagcta taaccaaagg ttattcttgc cattttgctt taagcaatga
     1741 attagtcaat cgactaaatc gtgcaaatca acgtggaaat ttagatcgat tgttaaaaaa
     1801 atatgcttct tacgatttac taattattga tgaaattggt tatttgcctt ttacccctga
     1861 cggggcaaag ttattctttc aattaattaa tctacgctat gaaaaaaaat caaccattat
     1921 tacaacgaat attcctttgt ctcaatgggc tgaaactttt caagataaaa aattaacaaa
     1981 tgcactaatc gatcgattag tgcaccacgc taagattatt caaattactg gaaagtctta
     2041 tcgaattaaa gattataaag aaaaaaagtt gtcgtataag taaccttgtt atttatgggc
     2101 caaaaaccta catttttggt ggccaaaaaa cgacatttct aaatggccaa aaaccta
//
LOCUS       DACENJ010000273         2115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.285, whole genome shotgun sequence.
ACCESSION   DACENJ010000273 DACENJ010000000
VERSION     DACENJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.285"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..538
                     /locus_tag="IW748_002540"
     CDS             <1..538
                     /locus_tag="IW748_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1105432.1"
                     /translation="IGYELYIKDFPEIPHKVFKDIQERYSNTFSLNKDYELLNPIIKN
                     IFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRKKLGLTMQEFGYEVSNSPRSTVSTW
                     EHGGNLPNKAKLKKIADIANCSVEDLLFDEGFISEKSQVKLINELKEENSKLKEKIKY
                     YECLFDGIESLLNSRKNS"
     gene            578..1189
                     /locus_tag="IW748_002541"
     CDS             578..1189
                     /locus_tag="IW748_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1105433.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
BASE COUNT          845 a          239 c          411 g          620 t
ORIGIN      
        1 tatagggtat gagctatata ttaaggactt tccagaaata cctcacaaag tctttaaaga
       61 cattcaagaa agatatagta atacgttttc gctaaataaa gactatgagt tattgaatcc
      121 tataataaaa aatatattta caaaatatta ctcaaaagat tttgaagatt atcgagacat
      181 agaagttaaa ccagacccta aaaaggtagg gagaaaaatt cgttctatta gaaaaaaatt
      241 aggacttact atgcaggaat ttgggtacga ggtttctaat tctcctagaa gcactgtgtc
      301 aacatgggaa catggtggta acttaccgaa caaagccaaa ctaaaaaaaa ttgcagacat
      361 tgcgaattgt agtgtagaag atttgctttt tgatgaaggc tttatttctg aaaaatccca
      421 agttaaattg ataaatgaac tcaaagaaga aaattcaaag ctaaaagaaa aaataaaata
      481 ctatgaatgt ttatttgatg gtatagagag tctgctgaac tcacgaaaaa attcctagtt
      541 aattaaacta gagtaaaatt atttgtaggt gttaaatatg ttagttggct atgcacgagt
      601 cagttcagat gatcaaaatt tggaaagaca gcttgaagaa tttagaaaaa ttggtgtcga
      661 aaaagtattt gctgagaaaa aatctggacg taacacagta gaacggccag agttcaataa
      721 aatgttgtca tttgtcagag aaggggatac gcttgttttt gagtcgcttg aacgcttagg
      781 aagaaattct gatgaaatac tagaaacagt atcttttata gataaaaatg gtatgggctt
      841 aatggttttg aatttaccaa ttttagatac aaaatttggt gatccaaact tagaaaaact
      901 tgttagaagc ttagtaatta acattttttc ttggacggct caaaatgaga gggaggaaat
      961 taagagaaaa caaaaacaag gaatagcaat tgcgaagaag aaaggagtct ataaaggaaa
     1021 accctacgaa tattcttcta ctgcaaagaa tccacaaaag aggtttatct ataatgaaat
     1081 agtaagaatg cttggagaag gtgttccgat taagcgaatt gcagaaaaaa caggaacaaa
     1141 tagaaattta gtctatagaa taaagaaaag aataagtgaa tctgattgat tttaagttct
     1201 ttgagaaatc aatgttacga gagaaggatt ctaagcctgt ttcaaacttt taaggtgtac
     1261 aattatacaa agaagcttct aaacaaaaga attaattttg tggcagtcaa gtaacgacag
     1321 aaatagaaaa gaaagaggct atgcgagagt gaatcaacaa gttaattggg ttaatgattt
     1381 tgaaaaagaa tttcgactga aattttttgg aaatcctaca gcagaaagta cagaagaaaa
     1441 aaattatgac tggtcagaat tttaggttgc atattcgatt caataagtgt tgaagataat
     1501 agttctctta cgccacagta tacaacgtta gaatatgacg aaaacagatt caatacattt
     1561 ccttgcaata ccattcaaga atcaattgat aagcatgagt gataattgat tatctacagg
     1621 aaggtgtttt ttatgaaact agagatcaaa gaagttgtct gcgattgggg aatttatgta
     1681 gacggagaaa catatccatt tatgattttt aatagtaaag caaatgcaca agagataatg
     1741 cgtatcatgg agttggataa taagcacgaa agatttgatt aagtaaaaaa atggagaatg
     1801 aatgtgaagc agtggaatac caaaaaacat gcgccaataa taagtatatt ttaggtgcaa
     1861 tgggattagg taaatcagct tctattaaac aggtctacga agaatcacag gagaattgcg
     1921 tatatgaaaa ttcgaattaa agagtgagaa tatgtgggag ctaatttatt tagttttata
     1981 cttaatagga aaatcaactg aatttgtata taacaataag gtaatattta tattgttctt
     2041 aatagtttgt attatcgtat ttgatgtcta tgatttacta attcataaga atgaaaatag
     2101 taatattggt agaat
//
LOCUS       DACENJ010000274         2108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.286, whole genome shotgun sequence.
ACCESSION   DACENJ010000274 DACENJ010000000
VERSION     DACENJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.286"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..425)
                     /locus_tag="IW748_002542"
     CDS             complement(<1..425)
                     /locus_tag="IW748_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1105434.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IW748_002543"
     CDS             complement(534..1565)
                     /locus_tag="IW748_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1105435.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          657 a          338 c          361 g          752 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tgagaattaa tcctttttta tataagttgt ttgcttattg
     2041 atggattgga taaattaaac catcacaacg agtggaaatt atatgaaatt tttcataaaa
     2101 attcatat
//
LOCUS       DACENJ010000275         2101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.287, whole genome shotgun sequence.
ACCESSION   DACENJ010000275 DACENJ010000000
VERSION     DACENJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.287"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..663
                     /locus_tag="IW748_002544"
     CDS             <1..663
                     /locus_tag="IW748_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1105436.1"
                     /translation="LEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDAKAAKY
                     VQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMTRGLAE
                     ISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLANMGMV
                     VEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKENEFSLT
                     IF"
     gene            678..1616
                     /gene="galU"
                     /locus_tag="IW748_002545"
     CDS             678..1616
                     /gene="galU"
                     /locus_tag="IW748_002545"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1105437.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            1741..>2101
                     /locus_tag="IW748_002546"
     CDS             1741..>2101
                     /locus_tag="IW748_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1105438.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKA"
BASE COUNT          750 a          367 c          456 g          528 t
ORIGIN      
        1 ttagaggaaa tcccagtaga aaaaagaaaa gctgttgttg ttctttctgg accaagccat
       61 gcagaagaag tagcgaaaca tgacatcact acgatcacag ctgcaagtac agatgcaaaa
      121 gcagcaaaat atgtacaaga gctgttcatg aataattatt ttcggatcta taccaatcct
      181 gatgtgatcg gtgtagagat gggggcagct ttgaaaaata ttattgctat tggtgctggc
      241 gctattcacg gcttagggtt tggcgataat gcaaaggcag caatcatgac gcgcggatta
      301 gctgagatca gccgtttagg tgtagcaatg ggtgccaacc cgctgacctt tatcggatta
      361 agcggagtag gcgatctagt cgttacttgc acaagtgttc attctaggaa ctggcgcgca
      421 ggcaacttgt taggacaagg gcagccccta gaagaggtgc tagccaatat gggaatggtc
      481 gttgaaggtg tgaatactac ccaagcagcg atggaactag cccaaataat ggaaatcgaa
      541 atgcccatca ctcaaacaat ctatgaagtg ctgtatgaac aaaaagaaat caaaacagcc
      601 gcaaaagaaa tcatgttacg tgatgggaaa aaagaaaatg aattctcatt aacaattttt
      661 tgaaggagcg aatgtatatg aaagtcaaaa aagcagttat tccagcagca ggattaggaa
      721 caagattttt accagcaaca aaagctatgg caaaggaaat gttgccaatt gttgataaac
      781 caacaatcca gttcatcgtt gaagaagcgt tgaaatcagg tatcgaagac atcttgatcg
      841 ttacaggaaa agctaaacga ccaatcgaag atcactttga ttcaaatatt gaattagaaa
      901 caaacctaag tgaaaaagga aagaccgaat tattgaaatt agtagaagaa acgacagatg
      961 tcaacttgca tttcatccgt caatctcatc caaagggttt aggacatgcc gttcttcaag
     1021 caagagcctt tattggaaat gaaccatttg tcgttatgct aggcgatgat ataatggaag
     1081 acgaagtacc attgacacag caattaatcg atgattacga acgtactcat gcttctacga
     1141 tcgccgttat gcaagttcct catgatgaaa cttcaaaata cggaattatc aatccaggag
     1201 aagtattgga agatgggtta tacaatgtca aaaactttgt tgaaaaacca gatccaagca
     1261 aagcaccaag tgatttagct attattggac gttacttatt gactccagaa atttttgatg
     1321 tattgtccga gcaacaacca ggagccggaa atgaaatcca actaactgac gcaatcgata
     1381 cattgaataa aacacaacgc gtatttgccc gtaaattcac aggtaaacgt tacgatgtcg
     1441 gtgacaaatt tggttttatg aaaacaagta ttgaatatgg tctaacacat ccagaagtag
     1501 gtgcaccttt acgtgagtac atcctgtctt taggtaaaac attagaagca gaaggaagca
     1561 agatcgatca taatccaaaa ggaataacag aaatcaaaga agaaaagaaa aaataaccct
     1621 tttttctaac gagcttcagc tgctaattga ataaaaagta gttgaagctc gtttttattt
     1681 gtttaaaaaa cacgaaaatg ctatccttat agaaaagaga gacaagaagg gagtttgatc
     1741 atgtcaggag gagaaattgc aggattgatc gcagcaatcg cctttgcgat acttgtcgtt
     1801 tttatcgttc gtgtgttgtt gcaagtttct aaaactttag aaaaagtaga tcagactgta
     1861 gcagaagcaa atacgacgat cgaaatcgtc acaaaagacg tagatctttt gtctagacaa
     1921 gtagaaggct tactggtcaa aagtaatgaa ctgctcttgg atatcaatgg aaaagtcgca
     1981 acgatcgatc cattgttcac agcagttgct gatttaagtg aaagtgtatc agaacttaat
     2041 cattctagta aaaacatcgc gacgaaagtc ggcggaatcg gtaaaacgac agctaaagcg
     2101 a
//
LOCUS       DACENJ010000276         2093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.288, whole genome shotgun sequence.
ACCESSION   DACENJ010000276 DACENJ010000000
VERSION     DACENJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.288"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..1204)
                     /locus_tag="IW748_002547"
     CDS             complement(26..1204)
                     /locus_tag="IW748_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1105439.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
     gene            complement(1634..2008)
                     /locus_tag="IW748_002548"
     CDS             complement(1634..2008)
                     /locus_tag="IW748_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105440.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT          594 a          388 c          349 g          762 t
ORIGIN      
        1 aattgtcctc ctgcaaaata attagttact cagtaaacat tgaaactaat gtatcggtta
       61 cctgttgaaa acctttatgg cttctgttta gaaatttttg attgtatgta tcaaaaatgc
      121 tgactagaaa gcgttctagt gattcttcat tttgaaactg ctcttttcta cggctgtatc
      181 ttttaatttg cttattgaaa gactcgatta gattggttga gtaaatggtt ctacgaatgc
      241 taggtggaaa atcataaaaa gttaataagt cttggttttc tatgagtgac tgcgtcactt
      301 taggatagtt tttcttccat ttctcaatca tgccggataa gaaggtattc gcttcttctt
      361 ttgagttagc ttgataaaca gccttaaagt catcacagat ttcttttcgg tctttgacac
      421 gtactttatg agcgatgtta cgagatacat ggatacaaca atgctgatat tttgctttag
      481 gataaatttg atggatagta tctttcatgc cttttaagcc gtccgtaata aaaagcaaga
      541 cttcttgaac tcctctggag ttaatatcct gtagcagctc attccaaacg tatgttgatt
      601 cagttggagc aatcgcataa ctcagtactt ctttagtgcc gtcttctcgt ataccaatgg
      661 caatataaat cgcttctttg gatacggttt gacgttttag tggaatgtaa gtagcgtcca
      721 taaaaatagc gacatactta tcatttaagg ctctggattt aaaggcattt acttcttcag
      781 tcagaacttt agtcatgttg gacatggttt gtggagtata gtgatgaccg tacatttttt
      841 cgatcaaatc agcaatttca gacatcgtaa cacctttttc gaataaatgg ataatagtgg
      901 tttccaatgt atcgtttgtt cttttgtagg ctggtaaagt ttgttgttta aactcaccat
      961 tacgatctct aggtatttcc aatgttaatt caccatattc ggttttgatt gatcgaaagt
     1021 aagaaccgtt tctcgaatta cctgaattaa aaccagtgcg atcatatttt tcgtaatcta
     1081 aaaaagccgt taattcagtc cgtaggagtg tgtttatcgc tttttctaag tgcgaacgga
     1141 ataattcatt taaatcgcct ttagtgacta gagtttgcac aatttctgta gtaaaatcat
     1201 tcatagggaa gtcctctttt ctgtgaattg gttgtcgtta actttattct acagaaggga
     1261 cttccttttt tgtatggatt ttttcattta cacaaaatat tttatactct caaaaactta
     1321 acaactttcg aaaactttcg tcacaaaata ttcacaacta atgactatta tataagcata
     1381 ccaacaaaca accctaacaa cactttttca tttttaactc cttatcctgc tagctccccg
     1441 gctagcagga ccttttttta tcaaaaaaac ttgtaccatc caatcgattc aactaatcaa
     1501 ccgaatgctg tcatgctatg atactcatcc taaaacgttc cgttttactg atatcagctt
     1561 aaagcattcg gttatttttg attagtaatg attgatggta caagctcata cgtgcttttc
     1621 tatctggtat aaattatttt gataaccgtt cctgttcccc tgctggtgaa aggattgtta
     1681 ttttttcaat ttgttctaca ggaaccatcc ggatctgctg ctcttcgttt tgtaggcgga
     1741 tcatcactcg gtctttttcg accgtttttg tcgccaccca gcctgagatc gtttcatact
     1801 tgctactcaa acgatttgct cttacttgta agataactag actatgttca gtagcagctt
     1861 tttgcagctc aagaataaac tttccttgca atcgtacatt cttagcatgt tcttcagtgt
     1921 catttacaaa agaatcaaaa agttgatacg aagtatgagt aactaacttc gtaagcttct
     1981 tcattggcga tactgtctct tcgttcatcg aatgcactcc tcctttagct aagaaagtgt
     2041 tacttaatta attttatttt acctaacatt ttacaaaaaa acaaggaaat gct
//
LOCUS       DACENJ010000277         2088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.289, whole genome shotgun sequence.
ACCESSION   DACENJ010000277 DACENJ010000000
VERSION     DACENJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.289"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            42..1742
                     /locus_tag="IW748_002549"
     CDS             42..1742
                     /locus_tag="IW748_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1105441.1"
                     /translation="MFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQ
                     TVKNKEQPTVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRN
                     LRIGLVAVSLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVS
                     RIIGEKEALSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTAL
                     VQIDLLEDSHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEA
                     SKKVMEYCRLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSS
                     IIFYQEIVPAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLS
                     REVVSVRLINELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPIT
                     ECSQAITDHEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKF
                     GDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL
                     "
BASE COUNT          736 a          326 c          426 g          600 t
ORIGIN      
        1 aaaagaaaaa caaaggaatc gaaatggtga tttctttgcc aatgtttgaa aaagaaaaat
       61 atgcattggg aattgctacg atagagaaac atacaataga acaagttgtt ggtgaaagtg
      121 gagaagacat actttctatt gaaaacgaag ttggcaacct ttttactact cagacagtaa
      181 aaaataaaga gcaaccaact gtattaacgg acgtggatct agattacaat cagctgaaag
      241 tgataagaga aaaagataaa acgtatatag tactgaaatc agattataat aattggattt
      301 acagattaga agttgactct agtgcaattg gcgcaatgat ccgcaatctg agaatcgggc
      361 tagttgcagt gtctttaaca ctggtaggac tcattggtct tctttcctac ttattttcgg
      421 acagatttgc ccgtccgatt tctcaaatac aggaacgtct gaatcttaaa tcaaaaggat
      481 tcagtgggaa agaactagat ttggtcgctc aatcagtcag tcggatcata ggagaaaaag
      541 aagcgttaag tgcacactta gttacccaaa agccacagct ggaaacgcta tttgtactta
      601 gtttatttag aaatcgagta gaaaggcgag aactagatca gcggttgcag caatttggtt
      661 atagttctca aaatgactgc tattatacag ctttggttca aatcgatctg ttagaagata
      721 gtcacggtgg ggagcgtgat ttgctacttt tagcgatcaa caacatgatc acggaaattg
      781 tgccaaagga aaatcgaatg ctgccgatcg ttttgaatga agaaatgcaa gccactattt
      841 atcgaatgga taaagatgat gcagaagcaa gcaaaaaagt catggagtat tgccgactca
      901 ttcaaaaacg tgtaaaagaa tacttgaatt taactatcag tataggcatc agtaaccggt
      961 tcgaaacgct gaatgaaagc aagcaatcgg ttgatcgggc aaaagaagcc ctgtattatc
     1021 gtgtaaatac tggtccaagt tcaattattt tttatcagga aatcgttcct gctgctcatg
     1081 aaaaaacact tattagatat ccgatagaag aacaaaatcg attgtttgaa gccattcgtt
     1141 ctggaaatca ggaagtgaag aatttagtac atgaattgat tgatgcattg tttgcacaaa
     1201 ataaaaatcc tttgagtcgg gaagtagttt cggtccgctt gatcaatgaa ctcgttcaat
     1261 taggacagtt attgggagta gattcaaaaa actttgataa catgaaacaa atttatgtaa
     1321 aagcactgaa caattatcat cccaaagaac tagaaaaact tgttttagaa caattagttc
     1381 tgccaatcac agaatgctct caagcaatca ctgatcatga atttaaaaat ttgtcggaga
     1441 atattatgca tatcgtctac acagaatatg atcgtgatct gtctttagat gtgatcgctg
     1501 ataggttgca ttataaccct aactatttaa gtaatgtatt taaaaaggaa acaggagaaa
     1561 aatttggtga ttttgttcaa aatcatcgtt tagaggtagc aaaaaaatgg ttgaaagaga
     1621 ctaacctctc cgtaaaagag attgcagaaa gattgcaata tcgaaatccg caaaatttta
     1681 ttcgtttctt taaaaagaag gaatcgatta cgccgggaga atatcgaaaa aaatatttgt
     1741 gaatcaagag gctgtttttc ttgtggactg attcaagcaa taaaagaagt tagaataacc
     1801 ctagtatttg tatttatgga tagttaaatt tcaaggctaa gcacgcaaaa agaactgtga
     1861 caaaagtctt gtcacagttc ttttttacga ataaatggcg ttcaagagac aggctatcga
     1921 caataaggca taattttgtg aaatatggga agctattttt caaaatcgct tcttattgct
     1981 cctgtgggtt actcgtcgca atgtttgtga gagtttttat catcgctcaa ggctgaacgt
     2041 ctctttcaca acctctgata cacggtgttc caggagtaga ttcttcga
//
LOCUS       DACENJ010000278         2088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.290, whole genome shotgun sequence.
ACCESSION   DACENJ010000278 DACENJ010000000
VERSION     DACENJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.290"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..199
                     /locus_tag="IW748_002550"
     CDS             <1..199
                     /locus_tag="IW748_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HAQ1105442.1"
                     /translation="GAVSPFGYEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDL
                     NKIIQLLEMPIFLMPANEKNN"
     gene            236..1246
                     /locus_tag="IW748_002551"
     CDS             236..1246
                     /locus_tag="IW748_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1105443.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1361..2074)
                     /locus_tag="IW748_002552"
     CDS             complement(1361..2074)
                     /locus_tag="IW748_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105444.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          685 a          350 c          438 g          615 t
ORIGIN      
        1 gggcgctgtt tctccgtttg gttatgaaaa acagattccg attattattg atgaagagct
       61 cttattaaat gaaaaacttg tttttgcacc aggtagacca gatcaaacga tggtcatcca
      121 aacggttgac ttaaacaaaa tcatccagct acttgagatg ccgatatttt tgatgccggc
      181 aaacgaaaaa aacaattaac caagtaaaat agtgcatact tggaggtgaa gcaacatgat
      241 aagttatcca ttattagaaa gaaatacaac gattggggtt actgcaccat cctcaggcgt
      301 atcagaggaa caccattcgt tactcgaatc agctttaaag agaatggaag aaaaatcttt
      361 ccaagtagtt actggaaaga cgctttggac gcaagataag gaaaaatcag gtccagcaag
      421 aaaacgagca gatgaattca atcaaatgat gcaagatgaa aatatccaaa tgatctttcc
      481 gccttgggga ggagaattat tgattgagat tctcgatttt gtcgattttg atgctctaca
      541 accaaaatgg atagtaggtt attcagatat tagtacgttg ctacttgctg tcacattaaa
      601 aacagggata gccactgcac atggaatcaa ccttattgat ttaagaggag agtacttgga
      661 tgaaacgaca gcaatgtggg aaagtgtact gaaaacaaga gaaggagaat cgattctaca
      721 gcaaccttct gaaaagtatc aaaaagagtg gccccatgag cattcatcat cttctgtttt
      781 ccatctcact gaaccaacaa attggcaatc agtcagcgga gatgcagtaa ggatgaaggg
      841 acgattatta ggtggctgta ttgatacgat cagtcattta atcggtaccc cttatggaga
      901 tgtccaatct tttaaaagaa agtataatga tggaaagccg atcttgtggt ttctagaaaa
      961 ttgtgagcta tcgtcagttg atttaagaag atcacttgtt cagatgaaac tggcaggatg
     1021 gtttgatggc agctcgggga tcatgttcgg tagaagtgca gcagaggatt tgttagatta
     1081 taaaatgaag gatgtatacg aagatctgtc aaaggaatta aaactaccga tcttgtatga
     1141 tattgattgt ggtcatgtac cgccacaact gacattgatc aatggtgcct atgcagaagt
     1201 agcggtagaa gatagaaaag cgactgtttt gcaaacgttt atttaaaaaa ttttttcctg
     1261 ccttggagtt tatcaaagac caacaaatct aaatagaaaa gaaaatgaaa gaagctgaca
     1321 gtattttgcc agcttctttt tgtatgatct attagaaatt ttaattatcc ataactatca
     1381 cattgatcag ctgtttcaca tcatttggat tacctgtagg aatagctact acagatgcat
     1441 tatcaataat ttcttgagtt gtttctctaa tgaacgctgg ataagatgtt tgatctgcag
     1501 gcatttgcca atgtgtagga actggataac tagtgattgt cccgtcatgg ttacttgaat
     1561 acactaaaac tccctggtaa ctgtatttcc cagtgagcat aaccacatct ttcggataga
     1621 ctgcactccc tgcatcataa ggggcgatag gtgttcctgc aggataatga ataacatgta
     1681 gttcgaaatc actcgatgta tctctaaaag tattacctct aatagctagc caaacacgtg
     1741 cgtactcaat ttgtagatca gaatatcctg ctaacggatc tattgactga gaagactgtg
     1801 aggaagttgt gtctattgtt gaagaagaaa tatttgtctc aactgtacta ctagatgtag
     1861 aagaagtatt gggctctgag actttactgc ttgttgaaga tgtattcgtt tcagtagaag
     1921 ctgatttctg agtacttgat ttttcagctg aactggtttg tctgattgaa cttttgtctg
     1981 tggtcgcaga tggatcacta ttttctttac taggagacgt acatcctaca aggaataaag
     2041 agaccaacaa taaaataaaa cctaactttt tcatataaaa tacctcat
//
LOCUS       DACENJ010000279         2071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.291, whole genome shotgun sequence.
ACCESSION   DACENJ010000279 DACENJ010000000
VERSION     DACENJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.291"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            249..1199
                     /locus_tag="IW748_002553"
     CDS             249..1199
                     /locus_tag="IW748_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1105445.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(1244..1877)
                     /locus_tag="IW748_002554"
                     /pseudo
     CDS             complement(1244..1877)
                     /locus_tag="IW748_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT          663 a          371 c          329 g          708 t
ORIGIN      
        1 tctgtctttt taagacctag tataaaaaga atgggttcat tttttctttc ttgcaggatg
       61 aggtttttta tataaacttt atttgatata aagaaaaggc attcataatt gtgctatact
      121 actaaaaaat gataatacag tgaattagat tagcgcttta ttaaattaag aggacagcat
      181 atcgatattg cttccttttt tttgctattt atttaaggga tagaagttta gaaaggactg
      241 tttataagat gaaaaatgat atagtgactt atgcaaacac aaaatgtgaa gaaattggca
      301 tcagtaattt agaagagtgc ttttttgaca caaatgacct gtcgcaattt ttaagaacgg
      361 gaagaacaaa tgccagtaaa cttgccaatg acttagttgc aaagaacgtt ctatttaaaa
      421 tcaatactcg tccagtgtta tttgccatca aagagcgctt tgaagagcac tttttgattc
      481 cggtaaaaga tgtatacgaa agtatatctg aattaaaaac agacttggag cccaattcta
      541 cagattccat tctacaaaag atgattgggt atgatatgag cttatccgat gcggtggaac
      601 aaatgaaaac tgctgtcttc tatcctggaa gagggcttcc tatgatgatc actggagata
      661 ctggagttgg aaaaagtttt tttgttcata ctgcttatca gtttatgaaa aaagtgaacg
      721 ttattcatga gaatgctcct tttaaagtac ttaattgtgc gcaataccat aataatcctg
      781 aattattgtc ggggatacta tttggctatg ttaaaggtgc ttttaccggc gcagatgaag
      841 atcgaaatgg attgttgcaa gatgcagacg gtggagtact gtttttagac gaagtacaca
      901 gattgcctcc tgaagggcaa gagaaactat ttgtttatat ggatggcgga acttattcta
      961 gagtcggtga aactatccat cacgcctcca atgtacgttt agtttttgcg actacagaag
     1021 gcaaagagga ttttctggaa acctttatgc gaagaatccc tatacatatc catatcccaa
     1081 atattgagga acgaggtatt ttagaaaaaa aacaaattat cgaaaatact ctctttagcg
     1141 aaagcaaaaa ctttagcaag gctattgatg tttctcctaa agtcactagt gttgcataaa
     1201 tatagtcaga atattctgaa ttattatttt cttgaaaaaa gtaaaaaaaa tcgtatccca
     1261 aactttcatc tacatttcca gaaagatctt catgttgata atcaattcct gaatcaatga
     1321 ccgctacctt cacatcctta gaacccttgg tttcttccca agcggatggc atgctaatct
     1381 ctcttaaatt agcttgttta ctccaattag gatcgttatc gatttcctaa agaatcagtt
     1441 gtatttggct cctcaacatc attcaacggc tctagttcaa ccacatagtt tggctctgca
     1501 taattgacca tatcagaagc attaaatgtt cttactgcct cttccacact aatttcttcg
     1561 ttcaatttca ctaataatac ttgatctcgc ttattataat cgctttcatt tgtcctaaga
     1621 gtcaaatctt tttccaccat aacttttaca cccaataaat tttgcggttt atttttctct
     1681 gttgaaattc tttgtgtagc ctttaaactg acaattagct cattgggtac atactctatt
     1741 tgagagtcat ctaattggct cataatcctt tctttttggt tagtatctgc agttacttgt
     1801 ttagtatgag ccatgaaccc aaacatactc accccaacaa ccaatcctaa atatattttc
     1861 tttttaaact ttttcatcta catctcctcc taatagtttt attttagagt tagctctatt
     1921 acttatttac taactcaaca acagattatc atacacttat taatgaaatc acaatatctc
     1981 attaaaacac accctaaaat cataaccgtt cctatgctca ttttttggat tttttaatca
     2041 cgtaaccaga aaaataaatc ccctctattt c
//
LOCUS       DACENJ010000280         2060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.292, whole genome shotgun sequence.
ACCESSION   DACENJ010000280 DACENJ010000000
VERSION     DACENJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.292"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            69..398
                     /locus_tag="IW748_002555"
     CDS             69..398
                     /locus_tag="IW748_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1105446.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            388..675
                     /locus_tag="IW748_002556"
     CDS             388..675
                     /locus_tag="IW748_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1105447.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(954..1517)
                     /locus_tag="IW748_002557"
     CDS             complement(954..1517)
                     /locus_tag="IW748_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1105448.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT          738 a          312 c          331 g          679 t
ORIGIN      
        1 tctactaaaa atagcgtaca atgtaattac taagtaatta cgaaatgtaa aataatggag
       61 gaaataatat gattacaaaa acaagaaaac aaggaaattc tattatgtta acggtcccta
      121 aagaattcga tgtgcccaat ggtgttgaag tagaagctaa gcttgtagag aatggtatcc
      181 tttatgagtt tgtagagcct aaaaaagaat tttttgattt tagtgaagat attttggctg
      241 atattctttc agaaggttat agcaaacaag agatcttaaa agaatttaga aatagaaaaa
      301 atgcgttaac ttctgcattt aaatctattg ctgaagatac tgttgcaaat tctaaaccga
      361 tgacaaaaga ggagttggca gcagaaattg gcttatgaga ttctaccttc taaacacgta
      421 ataaagtatc taaaaaaatt aaaagagaaa acattaaaag aacaattttt aacgataatt
      481 tatgatgaga tagcagttcg tcctcattcg ggagaacaaa aaacgggtga tttatcagga
      541 atttgggcta ttggattcaa gtatgcaggt acgacatatc gcgttgcata tgaaattaaa
      601 gataatgcag tgatacctat cttactatgt ggaacgcatg aaaattttta tgaacaatta
      661 aaaaagatta ggtaagaacc caatgtttca acttgagagg atagacgaca ctttaaaaga
      721 taatttttaa cagctgagag ttgtaatacc ctataatttt aaaccactaa cctatatatt
      781 tatgaaaaat agaaaaaatt aaaacgcatt ttaagcctct ttcaaattaa aagcttcatc
      841 cttatgcaca aagagggctt aaaatgcgtt attttatatt gttttttagt aataataaaa
      901 aaatcactct aattctccca taaatattat agattcctaa agattaaaat agattattcc
      961 ctattgtctt ttaagtagcg gtagagtgtt gccttgctaa taccagtcat ttccgtaatt
     1021 tcttttacgc tataaacctg acttttatac aattttactg ctcgatcaac atctttctgg
     1081 ttcactcgtg gacggccacc tttacgacct ctcgcacgtg cacttttcaa accttctttg
     1141 gtacgttcaa caattaaatc acgttcgaac tgactaaatg cttggaagac agtcatcatt
     1201 aagcgtccgt gaggcgtgct ggtatcgaag ttttctttca agctcaccag cttaacatgg
     1261 tgttcttcaa aataggtaac aaggtcaatc aaatcctttg tactacgacc caatcgagaa
     1321 aaactttcta caattaccgt atctcccgga cgaattttat cttttaaacg atttagttgg
     1381 ggacgaaccg ctttggttcc cgtcattttc tcagttagga tttcattaca attttgttca
     1441 ttgagtaagt ctaattgacg atttagttct tgtgaacgtg tactcactcg ggcataacca
     1501 taaatgtatc cactcatgct tttcttcctt tcgtacttta attctggctt gattgtatca
     1561 taaaagggag ttatagatac atagttaatg caacaagtta tgatacaaaa tatgacctct
     1621 tttatttaat aaaagaggtc atttcatttt gtctcataat cgattgtttt tgatacgagt
     1681 gtgtatacga aataaacaaa aaaatcttaa gtaaatttct agatacctaa tttaatccta
     1741 actagtgtat cctaaagtta ataacgagtt tatcacaatg aaaattataa aatgctccca
     1801 tattagatat aacatgcaat atttgaatta gaactacaaa atttattcgt tttataaagt
     1861 aaataatgtt gagatactaa ggaatagaat attataagga attgagctga atgtattgga
     1921 taaaaaaaga ataactttat tagagatgga ctataaaaat gctcgtaatt ttcttctgaa
     1981 gtctgaaagc tatttgaatg ctcagctacc agaatatatt agttttgacg aaactttaaa
     2041 atctgctaaa aaagttttaa
//
LOCUS       DACENJ010000281         2051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.293, whole genome shotgun sequence.
ACCESSION   DACENJ010000281 DACENJ010000000
VERSION     DACENJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.293"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..432
                     /locus_tag="IW748_002558"
     CDS             <1..432
                     /locus_tag="IW748_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAQ1105449.1"
                     /translation="KIDLPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEF
                     LIKDYAISKNSEDEDKIKKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHT
                     NKDVHDMDAFIKSAAQFIAADYDADLADQFINGNNFMPTTN"
     gene            complement(578..1054)
                     /locus_tag="IW748_002559"
     CDS             complement(578..1054)
                     /locus_tag="IW748_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAQ1105450.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(1014..1361)
                     /locus_tag="IW748_002560"
     CDS             complement(1014..1361)
                     /locus_tag="IW748_002560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105451.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(1348..1533)
                     /locus_tag="IW748_002561"
     CDS             complement(1348..1533)
                     /locus_tag="IW748_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105452.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1534..1806)
                     /locus_tag="IW748_002562"
     CDS             complement(1534..1806)
                     /locus_tag="IW748_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAQ1105453.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1803..2006)
                     /locus_tag="IW748_002563"
     CDS             complement(1803..2006)
                     /locus_tag="IW748_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105454.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
BASE COUNT          616 a          387 c          293 g          755 t
ORIGIN      
        1 aaaatagatt taccagaaaa tattgaaaaa gtttctccta catttgttga tatttatact
       61 caagccacac aagctaaatc tgagcaattg gaacaaattg ctggagttgg ttatagaaaa
      121 gctctagaat ttctaattaa agattatgca atatctaaaa attcagagga tgaagataaa
      181 ataaaaaaaa tgttattagg taaaacaatt gaaaattatt tgaatgattt tcctaaactc
      241 caaagattag ccaaagctgc tacttggatt ggtaatgatg aaacgcacta tgttcgtaga
      301 catactaata aagatgttca tgatatggat gcatttatta aatcagcagc gcaatttatc
      361 gcggctgatt atgatgctga tttagctgat caatttataa atggaaataa ctttatgcca
      421 actactaatt aaaagaataa aagagccaca gataccaatt aataaaacgg ttgggatatc
      481 ttggctcttt catatttttt tgacactaat agaatatcat gctgctaaca atgtttacat
      541 agctacaatg accgaacatt tagtgaacag tgtaatttta gaataaagag agttgttctt
      601 tgtacttctc gctattgtaa aaatcaattt cgttttcttt tttcaatcgg tcagcttgtc
      661 tgtgttcata ctcatctaaa aagttcaatg tctttctaat ttctgcatgt cgctgtctga
      721 catacgatga gctataccca acagattcag caatatcttc taagctcatt tgatcaacat
      781 atttcatctt aacaatttga ttatcgatac cagaaaagct atctatcagc aataacattt
      841 cttctttttg ctcgatcaac aattccaatt cattttctat cttttggata ttctcttcta
      901 aagaagaagt tctcgaattc ttttcgatgc ggacgtttgc taaatcacca cagacccatc
      961 gattcaattc aagcttactt ttattaagat tccactttaa gtaaagaatc tgctcatcta
     1021 gttcttggta atcttttaac caatgaaatc tcacaaacgc caccccttat ggtagaatag
     1081 tattgcgctg ctatctcgaa aggggtagct tttttatttt ttcttagccg ttccattctg
     1141 gattgccttt tgttccaaac gtcgtttttt cttcttaatc tttgattttg tttttcccat
     1201 acaattctcc tttccgaaaa cacattttgt tcgattttta atactaaata aaagttggcc
     1261 agaatttttc gttcgtcttt actcatcttt ttttgaataa gattactagc ataaaaatag
     1321 ttagtaccac gaaaatgaac aggtaaatca attacgttca ccttcttcat gccagttatc
     1381 agccactaga atctttgcgt tagtgccgcc accgttttct tttttgtatt tttcaaatgc
     1441 tttataagca gctgtttttg atttatagat ttttccggaa ccgactggga acatccatgt
     1501 gccctctgtg atagctaaga tgtatacttt catttaattc acccctttaa tccccaacac
     1561 tacatatccg gacttctgca tgtaatcagt aatataagtg atctctgctt tgtatgagtc
     1621 accagtgtaa ctaaatgttg gtctcgttag tccaggtaat tcatgtttaa tgtatttttt
     1681 atattcacgt agtattaact gatcaccaac tttgaagtca cgatcattct tgcggatctc
     1741 gaattgtttg cgtccgctcg tcactgcttc aaaatattct ggtagtattt ttagttcgtg
     1801 gatcattctg tttcctcctt caaaattcgc tcatcatcaa aaagtgaatc tccacaaaat
     1861 ccttctccca aatatttatc gttcatgtat aagtcaacca aatatttgtg atctgcttct
     1921 cgatattcat taatattaac gatctttatt ttgtaaacat tatcttcgtc gctcactatc
     1981 ttgaattgtt ctctgacttt caccaccgtt cctcctcttt ttgaaaaaat tttagtgttt
     2041 ttgtaagacc c
//
LOCUS       DACENJ010000282         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.294, whole genome shotgun sequence.
ACCESSION   DACENJ010000282 DACENJ010000000
VERSION     DACENJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.294"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW748_002564"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW748_002564"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1105455.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IW748_002565"
     CDS             complement(1431..1823)
                     /locus_tag="IW748_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1105456.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          452 c          342 g          662 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gcgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACENJ010000283         2030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.295, whole genome shotgun sequence.
ACCESSION   DACENJ010000283 DACENJ010000000
VERSION     DACENJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.295"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            16..822
                     /gene="proB"
                     /locus_tag="IW748_002566"
     CDS             16..822
                     /gene="proB"
                     /locus_tag="IW748_002566"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1105457.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            819..>2030
                     /locus_tag="IW748_002567"
     CDS             819..>2030
                     /locus_tag="IW748_002567"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1105458.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTK"
BASE COUNT          654 a          394 c          456 g          526 t
ORIGIN      
        1 tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta
       61 ggaacaagta cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta
      121 gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga
      181 gctgtaggcg ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa
      241 cagcaagctg cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc
      301 agtgtatata atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct
      361 gaaagtcgta aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct
      421 atcgtgaacg aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc
      481 gataatgatc agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc
      541 ctttctgata tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg
      601 tatcgctata tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc
      661 acatttggaa caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca
      721 aacagcgcaa tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca
      781 ggaaaagaag taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag
      841 gacaacaggc aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg
      901 agctacttgt tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa
      961 atcataaaga cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct
     1021 tgcagctgac acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt
     1081 tagacgatcc tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg
     1141 gcaaaaagcg cgtgccacta ggcgtgaccg gcatcattta cgaatctcgt ccaaatgtca
     1201 cgacagatgc agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca
     1261 aagaagcatt cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg
     1321 ctggcgaatc tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac
     1381 agttgatgcg attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa
     1441 tccaacgagt caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc
     1501 acgtatatat cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa
     1561 aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg
     1621 cacctttttt cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg
     1681 cggatgctcg tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg
     1741 atactgaata tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa
     1801 ttgcacatat caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg
     1861 cggctagtca acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca
     1921 ctcgttttac agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa
     1981 agttgcatgc ccgtggtcca atggggttaa atgaattaac ttccacaaaa
//
LOCUS       DACENJ010000284         2020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.296, whole genome shotgun sequence.
ACCESSION   DACENJ010000284 DACENJ010000000
VERSION     DACENJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.296"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            315..881
                     /gene="amaP"
                     /locus_tag="IW748_002568"
     CDS             315..881
                     /gene="amaP"
                     /locus_tag="IW748_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1105459.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            894..1082
                     /locus_tag="IW748_002569"
     CDS             894..1082
                     /locus_tag="IW748_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1105460.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1095..1652
                     /locus_tag="IW748_002570"
     CDS             1095..1652
                     /locus_tag="IW748_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1105461.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1684..1926
                     /locus_tag="IW748_002571"
     CDS             1684..1926
                     /locus_tag="IW748_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1105462.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          737 a          308 c          409 g          566 t
ORIGIN      
        1 agttatttat tgtatttttt aattctccga aaaaatttaa taaataaata gaatcttgta
       61 ataataactg agcataatct ttgcaatttt taatcttgaa taatatcctt taagtatagc
      121 cagcaatcaa aagtaaatag agaataagta agcaattgtt atttcagtat cagactggca
      181 ataaaacgcc gttatgatcc agcaatgttt ggtagcgctt tatttactga tgctatgcta
      241 actgttcgct atctatactg tgattgtcgg tagagttatt tacagggtaa aaataagttt
      301 ataggaggtg gaaaatgaat aaggggttaa aaataatcgg tgttatcatg agtttactgc
      361 tactttcagt atttctagcg attgcgttga tcaatgcgcc gtatattatg cctgaacaat
      421 tggaacgatt tcgcttcttt actttaacga attactacat gcaacaatat attttctggg
      481 cagctgtagt atttgccgtt cttgctatta tcatcttgct agtcgttctt ttctatccaa
      541 agtcaagagg aactttcgtt atgaaacggg aagatggcaa actaacaatc gacaaaaaag
      601 cgatcgaagg gttggtacgt tcccatttac acgaagaaga gttcattcat tcacctaaag
      661 tacggatacg ttcaacgaaa aatcgaatcc atatccatgt caacggagac ttgaaacgaa
      721 cttcttcatt agtagggaaa acaggtgtat tgatgcaaga tatcgaagaa gaagtaacta
      781 aagtactcgg aacaaaagaa acggtcaaag tggcagtcac atacagcggt tatgaagaac
      841 aagaagatac aagagactac aagcactctc gagtggaata ggagggccaa ataatgaaag
      901 aactattttc agcatacaag ctccctatta tctttggggg tcttggacta ctgctagctg
      961 ttttatggtt aacgattgga tttttcaaga cattgttgat cataatcatg acagcaattg
     1021 gcgcaggaat cggtttttat ttgaaacagt caggaatagt tgatcaattt tttaaatcat
     1081 aggaggaaac aaagatggaa aacaaaaaca tgaatacaac aaatggatca gttttggcaa
     1141 atgaaaaagg agaaaaccgc atgatgaatg gtgaattgac ttatgaagac aaagtcatcc
     1201 aaaaaatcat cggttatgca ttgaaagatg tagatggtct attgacatta gatggcggat
     1261 ttttctcaaa tatggctgaa aaattagtca acacagataa cgtaacagca ggtatcaata
     1321 cagaagtagg taagaaacaa gtagctgtgg atctagatgt gatcgtagaa tacggtaaag
     1381 acatcgaaga tatttacaac caaatcaaag aattgatcag tactgaagta aacaatatga
     1441 cacatttaga tgttatcgaa gtgaatgtga atgttgcaga catcaaaaca caagaagaat
     1501 accaaaaaga tcaagaaaca gtacaagaca aagtaacaga agcagcaaaa tccacaggac
     1561 aatttgcttc acgccaaaca gataaagcaa aatctgctgt aggcaaaggt gcacaaaaag
     1621 tcaaagaaaa taacgaacca cgtgtacaat aagaagtaag aagtcagtta ggaggaattt
     1681 aaaatgggat ttatttgggc attaatcgtc ggcggtatca ttggagcaat tgcaggagca
     1741 atcacgaata aaggatcatc aatggggatc atcgcaaaca tcattgctgg tttagtaggt
     1801 tcagcaattg gacaagcttt actaggaact tggggaccaa caattgcagg aatggctatc
     1861 attccatcat tgatcggtgc aatcatttta gtattagtcg tttcattctt tgcacgtaaa
     1921 ttataagcaa attgaagaaa aacttatgtt gaaatgaaga acaaatttga gaaccgtagc
     1981 gacactcgct gcggttcttt tttatatatt ttatatgaag
//
LOCUS       DACENJ010000285         2010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.297, whole genome shotgun sequence.
ACCESSION   DACENJ010000285 DACENJ010000000
VERSION     DACENJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.297"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..914)
                     /locus_tag="IW748_002572"
     CDS             complement(<1..914)
                     /locus_tag="IW748_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1105463.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IW748_002573"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IW748_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1105464.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          664 a          368 c          394 g          584 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa
//
LOCUS       DACENJ010000286         2007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.298, whole genome shotgun sequence.
ACCESSION   DACENJ010000286 DACENJ010000000
VERSION     DACENJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.298"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            34..1368
                     /locus_tag="IW748_002574"
     CDS             34..1368
                     /locus_tag="IW748_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105465.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1355..>2007
                     /locus_tag="IW748_002575"
     CDS             1355..>2007
                     /locus_tag="IW748_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105466.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIA"
BASE COUNT          672 a          297 c          346 g          692 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt attcgtttgg tttctaaagg
     1981 tttcaagaat gcagatttta ttgctat
//
LOCUS       DACENJ010000287         2005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.299, whole genome shotgun sequence.
ACCESSION   DACENJ010000287 DACENJ010000000
VERSION     DACENJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.299"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..134)
                     /locus_tag="IW748_002576"
     CDS             complement(<1..134)
                     /locus_tag="IW748_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1105467.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     A"
     gene            257..724
                     /locus_tag="IW748_002577"
     CDS             257..724
                     /locus_tag="IW748_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1105468.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(987..1469)
                     /locus_tag="IW748_002578"
     CDS             complement(987..1469)
                     /locus_tag="IW748_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1105469.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(1569..>2005)
                     /locus_tag="IW748_002579"
     CDS             complement(1569..>2005)
                     /locus_tag="IW748_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HAQ1105470.1"
                     /translation="PVKFEGVNQEIQKLAQTITETQREEMHKLGDLYPNQVINVSYGF
                     DGDRLEEKGSLMHMIGFATTKENPYADLEMLAIEEHTWAIFPNKGPFPQTLQDTWGKI
                     YAEWLPSSDYGLVEAPEISFTKWGEDFSNVYSEIWIAVKAKK"
BASE COUNT          584 a          446 c          325 g          650 t
ORIGIN      
        1 gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt
       61 ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt
      121 actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta
      181 catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat
      241 gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc
      301 ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat
      361 cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat
      421 ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt
      481 actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac
      541 aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg
      601 gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa
      661 aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt
      721 ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta
      781 ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag
      841 cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc
      901 tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa
      961 gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct
     1021 cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag
     1081 gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt
     1141 aattcttcag gcgattctgc taaagcgctt acaacaagat acgttccttt tgggaatggg
     1201 atgattcgcg tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt
     1261 ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat
     1321 gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa
     1381 ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct
     1441 ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca
     1501 tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta
     1561 ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa
     1621 tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaacccata atctgaagaa
     1681 ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct
     1741 ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac
     1801 ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag
     1861 cggtctccat caaatccata agaaacgttg attacttgat ttggatataa atctcctaac
     1921 ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga
     1981 ttgactcctt caaatttgac aggaa
//
LOCUS       DACENJ010000288         1993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.301, whole genome shotgun sequence.
ACCESSION   DACENJ010000288 DACENJ010000000
VERSION     DACENJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.301"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..378
                     /locus_tag="IW748_002580"
     CDS             <1..378
                     /locus_tag="IW748_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ1105471.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IW748_002581"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IW748_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1105472.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>1993
                     /locus_tag="IW748_002582"
     CDS             1859..>1993
                     /locus_tag="IW748_002582"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1105473.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     I"
BASE COUNT          553 a          338 c          430 g          672 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc ata
//
LOCUS       DACENJ010000289         1975 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.302, whole genome shotgun sequence.
ACCESSION   DACENJ010000289 DACENJ010000000
VERSION     DACENJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.302"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            16..903
                     /locus_tag="IW748_002583"
     CDS             16..903
                     /locus_tag="IW748_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1105474.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            900..>1975
                     /locus_tag="IW748_002584"
     CDS             900..>1975
                     /locus_tag="IW748_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105475.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSS"
BASE COUNT          613 a          383 c          463 g          516 t
ORIGIN      
        1 gaagagaggc tttttatgtc atttcaaatc ggggaacttt ttaatttgga ctattggcag
       61 caaatgtttt ccggtgactt gttctctctg aattttttgg tgaacatact tgatattctg
      121 gttgtctggt atcttgtata caaactgatc caactgctgc gaggcacaaa agcgattcag
      181 cttctgaaag gtgtagctat ttttattgtt atccgaatcg tagcggaaat cattggtctg
      241 cacaccttgt cttggctgat gaaccaagtc attacgtacg gggtgatagc tgctatcgtg
      301 atttttcagc cggaaatccg gagaggactg gaacatcttg gacgcagttc gcttttcaaa
      361 caatcaaaaa gcgaacagca ggaagatgag aagatgatct tgtcttttga caaagccatc
      421 caatatatgt ccaagcgaaa aatcggtgcg ttgatcacta tcgaacgtca tactggatta
      481 gatgagtata ttgagacagg gattgcttta gatgcagata tcactgggga gctgctgatc
      541 aatatcttta ttcctaatac accgctgcat gacggtgcag ttattgtaaa agaaggaaag
      601 atcgcagtag caagcgccta tcttcctcta tcagaaagta tgttgatccc taaagaattc
      661 gggactcgac accgggcagc tgtcggtata agcgaagtca gtgacgcgat taccatcgtt
      721 gtctctgaag aaaccgggga cgtcagcatc acgttagata atgaactgat ggctggctta
      781 tcccagcaag aatatttggc aatcttgcgt agagaactaa ttgctgaaga gaaaacaaaa
      841 aacaagaaaa acgtgattca atctttccta gacggtatga ctaagggagg ggatcgcaaa
      901 tgatctcaaa agagaaacgg acaagcattc tttacagttt tattgcattg ctgttcagta
      961 ttgcgctgta cttcaatgca aatggccagt ccgttccaaa tacgctttct ggaaatgaag
     1021 cgtataatca aacagttacg ggggtgccga tcaagatttc ttatgattca aagcgctact
     1081 atatccatgg atatgaaaat gcagtgactg tcaaactttc cagtgcaaac cgagttcagt
     1141 tgaatgcaga ggcaaacgaa gatacacgga tgttccgcgt cacagcagat atcactaagc
     1201 tgggcgaagg aacacatgaa gttccgctaa aaattcaaaa tttgagcagt gctgtcacag
     1261 caaagatcga accatcgact attacagtaa cggttgagaa aaaggtaata aaagatttcg
     1321 atgtggaaac gttgattcct tcaacgttca caccatctgg atatgaactt gatgatactt
     1381 ccgttagtcc taaaaaagtc tctatcacta caggtgacaa aacattggca gaaatcaaac
     1441 gagtagtagc tgatgtggac gcttcgatgg tcacagatga tgggattaat agtgaggtcc
     1501 caatacaggc gctgaatgca gcaggggaag tattaagtat cgtctctgat ccagtgcaag
     1561 taacagtgag agctgattcg atcaagccaa gcaagtcggt acgactttat gggatacagc
     1621 aaggaacacc agctgccggg gtagaaagct atgatttcag tttttcaaaa ctggaagcag
     1681 aagtaagcgg ctctagcgat ttgcttgcat cgatcggtga cagtattgct gtaccgatca
     1741 atgtctcagg tatcaatcat cgaacaactc gtaaaattga aataccagta gaagaggggt
     1801 taagtatcaa gcccgaatca gttagtgtgg agattacacc cgtgttgcaa acgtcaagca
     1861 gtagctctgc ttcgacgagc agtcggacac aagaaaatac aagtacgagt agaagtattt
     1921 catctgctga ttcaggaacg gaaccatcga caacaaccat ttcttccagt tcgga
//
LOCUS       DACENJ010000290         1968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.303, whole genome shotgun sequence.
ACCESSION   DACENJ010000290 DACENJ010000000
VERSION     DACENJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.303"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..884
                     /locus_tag="IW748_002585"
     CDS             <1..884
                     /locus_tag="IW748_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1105476.1"
                     /translation="FGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIA
                     EILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHL
                     LTLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDT
                     VIQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYR
                     INSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDV
                     VDKTVFEAFQRGLNKCD"
     gene            complement(973..1365)
                     /gene="rpsI"
                     /locus_tag="IW748_002586"
     CDS             complement(973..1365)
                     /gene="rpsI"
                     /locus_tag="IW748_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1105477.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1381..1824)
                     /gene="rplM"
                     /locus_tag="IW748_002587"
     CDS             complement(1381..1824)
                     /gene="rplM"
                     /locus_tag="IW748_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1105478.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT          634 a          359 c          357 g          618 t
ORIGIN      
        1 tgtttggtga gtatgcaatg aactatctaa attcaaatac tgagataata tcagatgaat
       61 taacaaaaaa atcgaaagaa tacgcaaata taaaaattat caaaggatat gtccgttcta
      121 tgtttgatat tgcagaaata ctaaattata ttgaatttaa ccgaactacc aaaattattc
      181 aatgtatcac tgcacctaaa aaaattgctt tagaagaaag aaggatacgg gaaggtaatc
      241 aagcattatc gtcagaagag cttattgctt ggatagaagc agtaaacact gacttgaata
      301 atcacttgct tactcttcat gactatactc ttttcatgct taccctatat cttggtgata
      361 gaaaaagtga gacgtatgct ttgcaatgga aatatatcga ctttgagaaa caaacggttc
      421 gccttaaaca tgctttggat aaatatcaaa aaaagaaatt tacaaaaggt cgaaaggata
      481 ccgtaattca agtacccgaa attgtaatgg cattactaag caaatggaaa tcagtgcaag
      541 ctgagcaatt gttgagatta aaaattaatc aaacaccaga tcagtatctc tttacctata
      601 ctaaaccatc cggagaagta aattgccctg ttcacgctga ttacctaaac tatcgaatca
      661 attcaattaa aagacgacat cctgaattgg ctcatttatc tccacataag cttcgtcata
      721 cctatgcaac tatagctaga caaggcggag caaatatgaa tcaaatatct agtgctttaa
      781 cacactcaga tatttctaca acgagaatat acgtaaatac accagatgta gtagataaaa
      841 ctgtttttga agcctttcag agaggattga ataaatgtga ttagtatgtg ttcttaaatg
      901 tgttcttaaa caagaaaaag acacttttca aacaattgaa aagtgccttg aaacattgat
      961 atataaataa tattaacgtt tagagaattg agatgcttta cgagcctttt tgagacctgg
     1021 ttttttacgt tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa
     1081 acggaagtca ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga
     1141 ttgtccagcg tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc
     1201 agtaacagcg aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc
     1261 aacatctttt ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac
     1321 tgcgttttta cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta
     1381 ttagattaag tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc
     1441 tccaccgtat acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat
     1501 accttttaca gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt
     1561 gattgatttc aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt
     1621 tttacctgtt aatttcactt tgtctgcatt gatgacgatg acgaaatcac cagtatccaa
     1681 atgaggtgtg aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag
     1741 acgtcccaat ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg
     1801 tttggccata tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca
     1861 tacacttcga gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg
     1921 ctaatctagt caatgtcaac gtttttctgc ttgattgaaa aatttata
//
LOCUS       DACENJ010000291         1966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.304, whole genome shotgun sequence.
ACCESSION   DACENJ010000291 DACENJ010000000
VERSION     DACENJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.304"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..204
                     /locus_tag="IW748_002588"
     CDS             <1..204
                     /locus_tag="IW748_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105479.1"
                     /translation="YSNSRLDAHFVCTSNPKLSFDAMTIVGNLNKNSAKKLSDFMSLD
                     PQIRLWDILQTKFKAKALQDPLC"
     gene            262..774
                     /locus_tag="IW748_002589"
     CDS             262..774
                     /locus_tag="IW748_002589"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1105480.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            771..1808
                     /locus_tag="IW748_002590"
     CDS             771..1808
                     /locus_tag="IW748_002590"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1105481.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          699 a          377 c          411 g          479 t
ORIGIN      
        1 tactctaata gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt
       61 gacgctatga cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt
      121 atgagtttag atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa
      181 gcactacaag accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag
      241 actatacgga ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg
      301 aaagcgacag ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt
      361 caaactttgg gcattccaga agcgactctt tgttcttggc gtaaaaaagc tagaattcaa
      421 gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt
      481 ttaattgtga tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag
      541 aagggcttat ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa
      601 caggaagaaa atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg
      661 cgtcaattag agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc
      721 ttattactaa gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat
      781 cttcagatcg caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc
      841 tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg
      901 aaggtacgat agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac
      961 tgacggatca agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt
     1021 tgccacctag ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt
     1081 ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag
     1141 tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt
     1201 gggatattac ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca
     1261 ttgatttatt tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc
     1321 atgccgaaaa acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg
     1381 ttttacactc cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa
     1441 aattaggaat tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg
     1501 aagcggcttt ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa
     1561 tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc
     1621 atagtgggat tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt
     1681 tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa
     1741 aaggtgttcg aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag
     1801 aaagttaaca ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg
     1861 aaaaagttat tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt
     1921 tttgataggt ggaaaataaa aatacgacaa ctatattgac aaacac
//
LOCUS       DACENJ010000292         1950 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.305, whole genome shotgun sequence.
ACCESSION   DACENJ010000292 DACENJ010000000
VERSION     DACENJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.305"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..234
                     /locus_tag="IW748_002591"
     CDS             <1..234
                     /locus_tag="IW748_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1105482.1"
                     /translation="AQIGQLSTEEWRRYRKYQQKIQNERTKTVYFDYLLQGKRKDRLQ
                     HELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS"
     gene            204..422
                     /locus_tag="IW748_002592"
     CDS             204..422
                     /locus_tag="IW748_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105483.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            442..1332
                     /locus_tag="IW748_002593"
     CDS             442..1332
                     /locus_tag="IW748_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1105484.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKTREQVQNIE"
     gene            1350..1634
                     /locus_tag="IW748_002594"
     CDS             1350..1634
                     /locus_tag="IW748_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105485.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT          714 a          327 c          416 g          493 t
ORIGIN      
        1 gcacaaattg gtcaattaag tactgaagaa tggcgaaggt atcgaaaata ccagcaaaaa
       61 atacaaaatg agcgcacgaa aactgtttat tttgattatc tgctacaagg aaaacgcaag
      121 gatcgtttgc agcatgaatt gcttaagcaa ccagttgaca cattactgat cctatattta
      181 tattatctca aggaggaaca accatggcac aagaattttc atatgaaatc gtagaagaaa
      241 tcgctatatt gtcagaaaac aacaaggggt ggcgtaaaga actgaaccta gtcagctgga
      301 atggtcgccc gccaaaattc gatcttcgag attgggcgcc agaccatgaa aaaatgggga
      361 aaggcctgac attgacgaac gaagagtttg aacaactgca aaaagcaata gaaaatatgt
      421 aatgaggaag gaccctgtcg aatgaaaagt atgactggtt ttggcaaagc catccgcgaa
      481 acaaaagcgt atcagttaga tattgaaata aaaagtgtca accagcgttt tctagatgtt
      541 caaatacgaa gtcctaaaat cctgaacttt gttgaaaacg attttcgtca gcttattaaa
      601 cagcagttaa gccgcggtcg aatcgaagta tttgtgaatc tagcttatat tggcaaaaac
      661 gaaaaacaga tcgtcatcaa ctgggaattg attgattcac ttatcactca attgacagac
      721 ggaattcaac aacgatacgg tagtcaaaat caagtgtcaa ttagtcggat ggcggaacgc
      781 ttcgcgctgg acgaaaactt cgtaacggta gaagaaaaac cggaagagag catggaagaa
      841 ttggcacagt tagctctaga tacattgaaa agcgcacttg attctattga ggcgagtcga
      901 gaaaaagaag gtcaagcttt agctttagtg ttgaaaaaga atactgaaga attcaaagaa
      961 gtactaaaaa aactcgatag ttttgtagaa atctatgaaa aggaatttca aataaggtat
     1021 caaaaaaagc tggaggaatt tcttggagcg aaagtcgatg agcaacggtt gttgacggag
     1081 ctagccatct tgttggaacg cggagatatt cacgaggaac tggatcgtct ggcgatccat
     1141 gtacaaaaac tagatgaact tatcgaagca caatgtcctg taggtcggga attagatttc
     1201 ctgatccaag aaatgaatcg ggaaatcaac acgatcggtt ccaaatcaaa tgcaatcgaa
     1261 attaaaaatc aagtcgtcca gtctaagacg atcttagaaa aaaccagaga acaagtacaa
     1321 aatattgaat gacgaaagag gtagagataa tgaaatatta tgacgtaacg ttccacgaat
     1381 taagtggaaa aactgtggtt aaacgggata tcccttctga aaaaaacggc tttgacgtat
     1441 ggaaagatgc ctgtgcagac tataatgaga acgaactgtt tattttgatc aacgacggtg
     1501 cttacgtgac gatgaatcgg aaatttattg ttcgaattga cacagaagag gtagaagatc
     1561 cgacagaaaa agcacgttct cgaaaagatg agatcatggg tgtagtgaat actttgtcca
     1621 atatgggctt ttagcaagga tgctgaacaa agaaatacaa taaataatca gaattggatt
     1681 tcttcgctta gcttctttgc tctaggaata catttaaagg ttgtgacaaa attcgtgtca
     1741 caatcttttt ttattttgaa taagtgatgt tcaaaaacca ttttctcggt attaagccaa
     1801 atttatgcaa aaatgtgaga aactattttc acaaaatttt tcttcatact cgaaactaat
     1861 cggcttcttc acgacctctt atacacggcg ttcaaaagct tgcatctgcg ataataaccc
     1921 caaccaaacg aaaaacatga gaagctgttt
//
LOCUS       DACENJ010000293         1949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.306, whole genome shotgun sequence.
ACCESSION   DACENJ010000293 DACENJ010000000
VERSION     DACENJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.306"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            595..1923
                     /locus_tag="IW748_002595"
     CDS             595..1923
                     /locus_tag="IW748_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1105486.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
BASE COUNT          655 a          320 c          383 g          591 t
ORIGIN      
        1 aataaagctt atgcaaagaa aaagaaatcc aatcacttgg gtttcttttt cttttattat
       61 tatgatgaat caagctttct gtttttgtca cgtatgttgt aatctttacg aaagagtaga
      121 gttggctact cataatcaac catacttccc atcatcgcta aatacgaaat aaatcagcat
      181 tcctacacca atcaaagcac aagctgcacc aagaaaaggg atgacattcc caaagttatc
      241 tgtattcata agtatgccac cacccacaaa caaaaagccg ccagcaatca ggtttttcat
      301 tttattcatc tcctttctta cgttatagta tttccttctt tcaatgagta ttttgaaagc
      361 tatcttattg tatgatgttc ttctaggcct cttctttatt atacaggcca ttttcgttat
      421 ctcatagaaa tattcctttt tatctgaaaa ctattgtata ccgtatgaca tttttcttca
      481 tgaagttgta agtatattct cattcaaatg aaaatttcat catagaagag attgtatttc
      541 taaattctta agagtaaaat acgaagtata acttggattt ggaaggatga ttggatgaaa
      601 gctattacgc ctgatatgac aacaaaattt aacaaacagt ttcaagcaaa tatcaaacag
      661 caggcattgc agcgggcagt tgtgaagaac ggaattactg cttctgctga aaatgtttca
      721 gcacatgtgg agaatacacc ggtattttct atagatttgg ctacggggaa agtggctaat
      781 caaatgcaat cggggcgttg ctggatgttt gctgcactga acacattccg tcataagatg
      841 ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa attacacttt tttctgggat
      901 aaatatgaaa aggcaaatta tttctatgaa aacatcattg caacaggaaa agaggcaatc
      961 gatagccgga aagtagcttt cttactcgct actcctcaac aagatggcgg ccaatgggat
     1021 atgatcgttt cattattcca aaaatatggc gttgtgccta aatcggtgat gccggaaagt
     1081 gcgaatagtt caaattctcg agatttgaat aactacttga acaaattact gcgtaaagat
     1141 gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat tagacgctat tgaagaaaag
     1201 aaagaagaaa tgctagaaag catttataac ttcttagcta tctcattagg aacacctcca
     1261 aaagaattcg attttgagta ccgcgatgaa gaaaagaact atcatcttga ccgaggactg
     1321 acaccacaaa ttttttatga taaatacatc ggtgtaaaat tagatgatta tgttagcgtg
     1381 atcaatgcac caacaaaaga caaaccattc aatcgttctt atacagtaga gatgctagga
     1441 aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg atatggaaac tttcaaaaaa
     1501 ttagcgattg ctcagcttga agaaggagaa tctgtttggt ttggttgtga tgtaggtcaa
     1561 tcttctacta gaactagtgg catcatggcc ttagatgctt attcaatgga tgatctattt
     1621 gatacggatt ttacaatgac taaagctgaa cgtttagatt ttggagaaag tctgatgacc
     1681 catgcgatgg tattgaccgg tgttgactta gtagacgggc aatcaacaaa atggaaagtc
     1741 gaaaatagct ggggcgaaaa agttggaaag aatggcttct ttgttatgag tgatgcctgg
     1801 atggacgaat atacgtacca aatcgttgta agaaaagatt tattgacgaa agagcaatta
     1861 aatgcgttca atgaagaacc aattgttcta tctccgtggg atccaatggg tgctttagca
     1921 tagacgagta tttcaatgaa gagtatgta
//
LOCUS       DACENJ010000294         1911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.307, whole genome shotgun sequence.
ACCESSION   DACENJ010000294 DACENJ010000000
VERSION     DACENJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.307"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            108..1199
                     /locus_tag="IW748_002596"
     CDS             108..1199
                     /locus_tag="IW748_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1105487.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            1229..1906
                     /locus_tag="IW748_002597"
     CDS             1229..1906
                     /locus_tag="IW748_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1105488.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
BASE COUNT          628 a          311 c          446 g          526 t
ORIGIN      
        1 aaaataaaat tttttaagca aattagttgc atgtacaact aatcgagtgg tttaatgata
       61 gatgattctc gataggttaa tcagagcgaa ttatgggagg aatgacaatg tcacaatttg
      121 atcaatcaat tactttacct tcaggagttc aattgaagaa ccgattgatg ctgtcgccta
      181 tgacgacaca actcagcttt ttcaatggag tgatcaccga agatgagatc acttattata
      241 aacaacgttc taaaggtctt ggtgctgtta ttactggtgc tgcaaatgtc caagatatcg
      301 gaaaaggctg gccaggtgaa ctcagcatcg ctcatgatga aatgattcct cgattaagtg
      361 agctagcgaa agcaatccaa ggagaaggcg caaaagcgat tattcagatt ttccacggtg
      421 gacgaatgac aagtcgagca actttatctg gagaacagcc tgtatctgca agtgcagttg
      481 cagcaaaacg tcctgatgcg gaaacaccac gagcaatgag tgaagaagaa atcgttgaaa
      541 caatccaagc atttggcaaa gccacacgta gagcgattga atcaggattt gatggaatcg
      601 agttgcatgg agcaaatacg tatttgatcc aacaattttt ctctccgcat tcgaatagac
      661 gagaggatca atggggcggt agcttggaaa atcgttatcg ttttattgaa gaattattaa
      721 caacggtttt ctcagcggtt gaacaatatg ctaaaaaacc attcatcgta gggtatcgtt
      781 tctcaccaga agaatatgaa acaccaggta tccgttttga agacacgatc tggcttcttg
      841 aacgattacg tgagtcaaaa ctagattatc tgcatgtttc attaaatatg tatgatcgaa
      901 tagctcgttc tgataaatac aacgataaat cgattcttga agtggtccat aacacgattc
      961 aaggaaagat ccctttagtt ggtgtcggtg ggatccgcaa tcgcgaagat gtagagcagg
     1021 tattgaaata tgcagaactt gcagcaattg gacagcaaat gatcgtcgat cctgactggg
     1081 cagagaaatt attggaaaac cgtgaggatg aatttgtaac gaaacctttt gaagaagcat
     1141 ataaagagct ttatttgcca agcccattat ataatttctt gaaagcacgt tatcaataac
     1201 gcaattttta tagaaagagg atgggaaaat gaaagtattg atcgtattaa caaatgtaga
     1261 aaagtacgaa aagatcgagc gtcctacagg tttgtggtta agtgaactga ctcattttta
     1321 tgatgtatta gtaaaaaatg ggatcgatgc agattttgtc agtcctaaag gcggatatgt
     1381 acctttagaa ccgcaaagtg ttttgaatat ggatgaaatc gattgggcct attatgaaga
     1441 tgaagatttc agaaatcggg ccttgggaaa taccttacgt cctgatgatg tggatgcaaa
     1501 tgattatgca gcggtttatt atgctggtgg ccacggaaca atgtgggatt ttccagaagc
     1561 aatagatctt gggaaagtcg cttcacaaat ctatcgaaat gaagggctgg tctcagctgt
     1621 ttgtcacggt gttgtaggat tgttgccagt tacagatgag tctggaaaat ctattttagc
     1681 tggacgtaca gtgactgggt tcagtaatga agaagaggaa gcaaatggta caacgaatga
     1741 agtaccattc ttagcagaag atgctttgaa agaaaaagga gcaaactatc agtctggagc
     1801 agctttttca gaagtggttc aaattgatgg gcggcttctt acagggcaaa atcctcaatc
     1861 tgctcatcaa ttaggcgaag cagttgttga agcattaaag aattaaaaat a
//
LOCUS       DACENJ010000295         1910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.308, whole genome shotgun sequence.
ACCESSION   DACENJ010000295 DACENJ010000000
VERSION     DACENJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.308"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            175..387
                     /locus_tag="IW748_002598"
     CDS             175..387
                     /locus_tag="IW748_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1105489.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            389..>1910
                     /locus_tag="IW748_002599"
     CDS             389..>1910
                     /locus_tag="IW748_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1105490.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQR"
BASE COUNT          603 a          384 c          409 g          514 t
ORIGIN      
        1 cggttgaaaa aatatccgaa cagataagca aaaacgaggc ttgtctcttt gactcgaatc
       61 cagtattcta gtcaaagcat atgaaaaacc aggtggcttg tcccctcgtt ttgtgttgtc
      121 taaaaaaatc aactagctaa atcactgctt tacgcagata aaggagacaa aacaatgatc
      181 tataaagttt attatcaaga aacaaaagta cgcaacccta aacgggaaga aacaaaatct
      241 ctttacattg aggcaaaaag cgatgtagac gctcgccaac gtgtggaaga aaatacccca
      301 tacaatattg aattcgttca acttctagaa ggaagtcatc tagagtacga aaaagaaaat
      361 gctgatttct cattagtgga gttttaatat gaaactttcc atcaaaaata acgagacagg
      421 cgtctttgct attggtggat taggtgaaat cgggaaaaat acgtatgggg tacaattcca
      481 agatgaaatc attttgattg atgccgggat caaattcccg gaagacgatt tacttggaat
      541 tgactatgtc attcccgact acagctacat catacaaaac ttacacaaag taaaagcctt
      601 ggtgattact cacgggcacg aagaccacat cggcggaata ccctacttgc tgaaacaagc
      661 aaatattcct atctatgctg gtcctctcgc attagcgttg atcacaaaca aattagatga
      721 gcatggcttg cttcgagatg cagagctcca tgagatcaat gaagatacga tcatccgttt
      781 tagaaagact tctgtcagct ttttcagaac gacccatagt atcccagatg cattagggat
      841 cgtggtgaaa acaccttctg gaaatatcgt agcgactgga gacttcaaat tcgatttcac
      901 tcctgttggc gaaccagcaa acttgcataa aatggctcgt atcggggaag aaggagtctt
      961 gtgcctttta tctgatagta caaatgctga gattccaaca tttactaaat cagaaaaaac
     1021 gattgggcat tctattttga agatcttttc aaaaatcgaa ggacgtatca tttttgccag
     1081 ctttgcttct aacatcttcc gtttgcagca agcagcagat gccgctgtgg caactggtcg
     1141 taaaatcgct gttttcggac gaagcatgga aaatgccatc gtcaacggtc aacgtttagg
     1201 atatatcaaa gttccagacg gcacatttgt agatgcacac gaaatcaatc gcttgcctgc
     1261 caatgaaacg atgatcctat gtacaggttc tcaaggagaa gcgatggccg cgttgagccg
     1321 catcgcgaat ggtactcacc gtcaaatcca aatccagcca gacgatacag tgatcttttc
     1381 aagttcacct attcctggta atacaacaag tgtcaatcat ttgatcaatt tgctgtctga
     1441 agctggtgca gaagtgattc acggaaaaat caataacatc catacttccg gtcacggtgg
     1501 acaagaagaa caaaaattaa tgcttcgttt gatgaagcca aaatatttta ttcctgtcca
     1561 cggagaattc cgcatgttga aagtacatgc ttctctagca caagatgtag gtgttcctgc
     1621 agaaaactgc ttcatcatgg aaaatggcga tgttttagcg ttaactagcg attctgcgcg
     1681 tcctgctggg cattttgtgg ctggagatgt gtatatcgat ggtagcggga tcggtgatat
     1741 tggtaatgtc gttttacgtg accgtcacct gctttcagaa gaaggtcttg tattagctgt
     1801 cgcgacagtg gatatcaaga aaaaagaaat cttggctggt ccagatattc tctctcgtgg
     1861 atttgtctac atgcgagaat caggagaaat gatcaatgaa gctcagcgaa
//
LOCUS       DACENJ010000296         1905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.309, whole genome shotgun sequence.
ACCESSION   DACENJ010000296 DACENJ010000000
VERSION     DACENJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.309"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            126..722
                     /locus_tag="IW748_002600"
     CDS             126..722
                     /locus_tag="IW748_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1105491.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            735..1634
                     /locus_tag="IW748_002601"
     CDS             735..1634
                     /locus_tag="IW748_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1105492.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1637..1905
                     /gene="ssb"
                     /locus_tag="IW748_002602"
                     /pseudo
     CDS             1637..1905
                     /gene="ssb"
                     /locus_tag="IW748_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734556.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          703 a          300 c          398 g          504 t
ORIGIN      
        1 aagaaccagc agaggattca aaaagaaatc gaattagatc gataaaatct tcctaactat
       61 tgtatacgta taaacttatg ttatactgtt tttataagtt tatacgtata caaaggaggg
      121 agaaaatgac aaaagcaatg gtatacaagc tgctagaaga atttaatgga actttggctt
      181 tgaaggatgc caaaaaagcg ggcatttctc ctgtaacgat caaacgtatg gtagatcgag
      241 gggagctgga tcgagagtat ccaggttttt tcaccttacc tggtcaattt cctgatgaat
      301 tgtttatggc gcaaaaaaaa tatgaacgcg gcattatttc acatattacc gcgttagatt
      361 tgtatgattt aacagatatg atccctagac agatcgatct gactgtacct tacggttatc
      421 acgtttctga aaaaggattg aaggaattcg cagttgagct acactataca aaagcggaat
      481 ggtatgaatt aggtaagatt gaaataaaaa gtcgctatgg gaatcctatt atagcttatg
      541 atccagaaag aactttatgt gacatttgga atccctggta taccgttgag gatgaaatca
      601 aagtaaaagc aatcaaaaac tatatggagt cggacagaaa aaatcttaga aaattgaatg
      661 agtatcgacg aattttacca acagacaaaa cgatgcgttc ttatattatg gctttaaatt
      721 aaaggagaag atacatgaca cctacgcagt taaaagcaaa agtgaagaat atttctagag
      781 aaaaagaagt tgatccgcag cttgttttaa gacattttat gatggaaaaa ttcttggaaa
      841 aaatctcaga gtctccttat cgtgaaaatt ttgtgttaaa aggcggcttc ttgattggct
      901 caaagtatgg cattgaaaat cgcacaacca aagatattga tacgacttta cgggaaatga
      961 aagtaaccaa agaaacatta acaacggttc taaacgaaat tttttcaacg ccgacgaaag
     1021 aagacattca atttgagatt caaggaatga aagaaacgag agaagccgat tactatccag
     1081 gatttagctt acgtgtgctg gcacatttgg aaaacatgcg gccagatttt aaggtagatg
     1141 tgactacagg tgactcgatc tatcctgcaa cgatcactca cagtcataaa ttgatgtttg
     1201 aggatcgaac gattcctttg gaatcctatc ctacagaaca aattattgct gaaaaattaa
     1261 gtgcaacctt tgatttttta acagataatt cgagaggaaa agatttttat gatctctata
     1321 ctattcctaa aatggagaag atcgatgaga agaccttgaa agatagtgtg agaaatacat
     1381 ttacacgtcg aggaaaaact gatccgttaa agatatacta tgaaaaggat atggatgtac
     1441 tgaaaaatag tcacgagcta aaagatacct ggaagaaata tcaaaaaagc aatccttatg
     1501 ctgcgtcgat cacgtacgat gagacaatgg attcgattag taatttgatg gaaaaagtga
     1561 ttcacgtcga agaaaaagag cgaaaactag cttatcaaag acaccaacag atgaatagag
     1621 aaatggagag ataaagatga ttaataatgt gacattagta ggacgcttaa ccaaagatcc
     1681 tgatttacgt tataccgcaa gtggtactgc agtagcgact tttaccctag ccgtcaaccg
     1741 aaatttcacg aatcaaaacg gcaattgaga agccgatttt attaactgcg tgatttggcg
     1801 caaaccagca gaaacaatgg cgacattagc taaaaaaggt agcttgattg gtgtagttgg
     1861 gcggattcaa acgcggactt acgacaacca gcaaggacaa cgtgt
//
LOCUS       DACENJ010000297         1898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.310, whole genome shotgun sequence.
ACCESSION   DACENJ010000297 DACENJ010000000
VERSION     DACENJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.310"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..518
                     /locus_tag="IW748_002603"
     CDS             <1..518
                     /locus_tag="IW748_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1105493.1"
                     /translation="YLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQ
                     YGIAVHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVH
                     LSPGVCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPA
                     KIKKEGINFEF"
     gene            508..1650
                     /locus_tag="IW748_002604"
     CDS             508..1650
                     /locus_tag="IW748_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ1105494.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1663..>1898
                     /locus_tag="IW748_002605"
     CDS             1663..>1898
                     /locus_tag="IW748_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1105495.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFII"
BASE COUNT          671 a          253 c          394 g          580 t
ORIGIN      
        1 aatatcttgg gaaaataaac gaattgaata gctgggcgga tagcaaaaaa aaaatctata
       61 aatttattgt agctattggt gactgtgtag taagaaaaaa gattataaaa aaattgaata
      121 tgccaagtga gcaatatgga atagctgttc atccaagtgc tatagtggca aaagaaattt
      181 caattggtta tggaactgtt atttttgcga atgctgtaat taacccagat gctgtaattg
      241 gagagcatgc tataataaac acaggtagca ttgttgagca tgattgtaga attaataact
      301 atgttcattt atctccaggt gtatgcttag cgggtggagt acatgtaggt gtaggaactc
      361 aaataggaat tggttctcaa tgtatccaga tgaagaaaat tggatcttgg tgtatgatag
      421 gcgcagggag cactattgtt aaggatattc cctcacattc cttagcttat ggcaatccag
      481 ctaaaattaa gaaagaagga attaattttg aattctagaa ttttattatc aagtcctcat
      541 atgggaggaa cagaagaaaa atatgttgct aaggctttta aagataattg gatagcgcca
      601 ttaggtgaaa atgtggataa atttgaagaa agtattacaa aatatactgg ttctaagggc
      661 ggagcttctg cgttaagttc aggtacagct gccatacatc ttgccttaag actgcttaat
      721 gttggaaaaa acgatattgt ctttgctcct tcatttactt ttatagctac agtaaatcct
      781 attttatatc aaggggctgt accagtgttc attgactcag aacctaatac atggaatatg
      841 agccctgttg cactagagag cgcattagaa gaagctgaaa gaaacaacca attacctaag
      901 gcaatcatcg tagtgaattt atatggtcaa atggctgatt atgaacgaat tttagctatt
      961 gcaaaccaat ttaatgttcc agtgatagaa gatgcagcgg aatcgttagg ttctacttat
     1021 aagggaattt cgtcaggctt atttgggaca attgggatct attcatttaa cggtaataag
     1081 atcatcacaa cctcaggtgg aggagctcta gtgtctagtg ataaaaaata tgttgagcat
     1141 tcaagatttt tagcaacaca agcaagagat caagcaccgt attatcaaca tagcgaaata
     1201 ggttataatt accgtctaag caatatttct gccggtatcg gacgtggaca aatggaagta
     1261 ttgaatgata gagtagctaa acgaagagaa attttcaatc gatataagga agctttttct
     1321 ggaattgata gaattgaaat gatgccagaa gtggatggca tggtgccaaa tcgttggttg
     1381 tctactttaa cgatcaatga ttataattct attgatcaag tagttaacgt aataaataaa
     1441 ctaaatgaaa aaaacattga aacaagagct ttgtggaaac cactacattt gcaaccagta
     1501 tttcaagggg taaaaatgta ttaccatgat aataatctta gtgtgtcaga aaagttattt
     1561 aatagcggct tatgcttacc ttctggaagt aatatgacag atagtgaaca agaatttgtt
     1621 attgatgagt ttaaaaaaat attcaagtag gtgtgatatt ttatgaacat aggaattatt
     1681 ggtttaggtt ccatggggaa aagaagactt agactcattc aagaacactt taatgatatt
     1741 ttactatgtg gtgttgatac taatgaggcg agacgaaagg aagttgaaaa ggaatttgga
     1801 ataccaactt ataatagtct aacagaggca aaaaatttgt ttgaaataaa ttcagttttt
     1861 gtttgtacat cccctataac ccatgaattt attataaa
//
LOCUS       DACENJ010000298         1877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.311, whole genome shotgun sequence.
ACCESSION   DACENJ010000298 DACENJ010000000
VERSION     DACENJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.311"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..127
                     /locus_tag="IW748_002606"
     CDS             <1..127
                     /locus_tag="IW748_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ1105496.1"
                     /translation="AKQKKMTKVLINAEGKLSHPLSIHVRNEWQLADSQLLFQEK"
     gene            273..566
                     /locus_tag="IW748_002607"
     CDS             273..566
                     /locus_tag="IW748_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105497.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            559..855
                     /locus_tag="IW748_002608"
     CDS             559..855
                     /locus_tag="IW748_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1105498.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            930..>1877
                     /locus_tag="IW748_002609"
     CDS             930..>1877
                     /locus_tag="IW748_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1105499.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDG"
BASE COUNT          715 a          257 c          366 g          539 t
ORIGIN      
        1 tgcgaaacaa aagaagatga ccaaagtatt gataaatgca gaaggtaagt tatcccaccc
       61 actaagtatt catgttcgta atgaatggca attggcagat agtcaattat tatttcaaga
      121 aaaataacat tttattttct catgttactt ataagtagtt ttacatgctt tttgacttta
      181 aaaatacatg tgatgataca tgttatatga attatcaatg tacccagtaa tatgctattt
      241 tcattatgta aaaaataaca gggggtgtgt agatggagat tgatgaaact tgtttgagta
      301 actcttataa aagtatgaat gacttttttg aggaaatgga actggattca gaattgctct
      361 atcaatttta tcttgctttt cgagggcaac agatttcttt tccaatgaaa atgtacgatc
      421 gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca ggaaaaaact gtagatatcc
      481 gtcaattgac agaagtttat ggtttctctg caagatggat tcaaaaagta attaaacaaa
      541 aagaaaggag acgagtacat ggctaagctg attgtattag gtggtttaat ttacggtgga
      601 tattggttat ataaatatat gcttgcgcat ccgaatatag ataaaggaaa agtaattaag
      661 acagttgggg taatcatagt tggactcata gctctaacta agaaaaataa tactaatagc
      721 ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa gggaaatttc tactagtttt
      781 aatccatctg agagtcctca aggaagagac cgctgccata cgggctatcg acattattgg
      841 gaaagaatcg attaaaagat taccatttag atcaattaat tgtctgatag aataagtacg
      901 tgagggagaa attgaaaggg ggaaatataa tgaaaaaatg gctgttatta ctaataggaa
      961 tgggaatttt attcactggt tgtacatctg agaatacgag gactaaagcg actatagaaa
     1021 ctgatacaac aattgagact aaaaaaagta aaacgcaatc aactgaacaa actacaacag
     1081 aaatgaagca atctgtggaa acaagtgcaa gttcaatcac tgaatcttct tctgaagagg
     1141 ttaagaatac tttgtgggat acgaataaag cttccaaatt agaaactttt gtcacacaat
     1201 ggggaaagac attaggtcaa gaatataaaa gctacaatct tcagaataat gtaagtctgt
     1261 atggcactcc tttaccacaa gccgtgatta atggcgattg gaaaatggca atcaatgaag
     1321 caccagttac cgtacaatgg tcagaagatg gaactggtca tgcagatttc aatttagtag
     1381 cagtttattc tgatgtagaa actggagaat atttagggca gcatgtttac tttttcggtt
     1441 ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa tcaaggtaat gaaaataatt
     1501 atctgtactt taacgaaact caaaatcaac agttaaaaca aggtttcagt gatattgtga
     1561 atggacaaac tcctcaaaca ccagtagtag aagaatctac tcaacaagca acaactacaa
     1621 cgacacaatc tacaagtagt tcaggaattt caagttggga agctgctaaa gaatctgtgc
     1681 taaataataa agctttatgg ttgtatgatt ctcctgataa aaatgtatca aatactaatt
     1741 taattagcac aaatgaatat atgaaagatc taatgcaaga tgaaaatgga agctactatt
     1801 caattaatat tgtaacggat gatgatggaa acacgaccat ggctggtgga aaacaatttc
     1861 gtgtttatac agatgga
//
LOCUS       DACENJ010000299         1870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.312, whole genome shotgun sequence.
ACCESSION   DACENJ010000299 DACENJ010000000
VERSION     DACENJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.312"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            291..1226
                     /locus_tag="IW748_002610"
     CDS             291..1226
                     /locus_tag="IW748_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ1105500.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            1270..1539
                     /locus_tag="IW748_002611"
     CDS             1270..1539
                     /locus_tag="IW748_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105501.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
BASE COUNT          670 a          251 c          372 g          577 t
ORIGIN      
        1 agaagtgaat tatctaacac aacaatatct gagttgaaga atggtagaaa gagtttagat
       61 accttaaaat tcagatcatt tgaagctcta tatatagctg ctaaaaaata ttttacagct
      121 gataataaat aaaaaattaa ggtgggttct atgtgaaaaa aacgtatcaa aagtttttat
      181 ggattagcta tatcgtccta tttatcgttg ggtttctcgt cagtgtgtcc gtgatcgtcg
      241 gaacgctttt tcttttgttc tctgacgaag atctggtact taaggggtta atgagtacgt
      301 tatcgtgtgt catgttactt tatttatgta tacagatcaa cgaaattatg ccccctcatt
      361 ggcaagaaaa gaagaaagaa tttaaggaag aaatcgaaat aaaatccaat gactggattg
      421 aatacacatg gcttcctctg aaaagaaata gcaaacgaaa ggaattttgg gcattgcaat
      481 tatggccaat aggaaaactt tttatttata tggcttcact tatggttact ttaatcggtt
      541 atttggtggt ggtctatggg gtggcctatt acttgttaag tgagccaaaa cagatcgcgg
      601 cgtcaaatat tttcacggca gtaatgacat taatgtgtgt catgtgcttt tctattttta
      661 gttacttact cgtccaattt atcaggcaaa atttaacaat gagtaaactt ttcaaaagtt
      721 tattggactt acttatctta ggcagtatgt taacgactct aacagatcgg ttcgaacaat
      781 ctgaaaaaac ggtagagggg ataaagaact tcttaacgac gattgatcaa tttataaatg
      841 gatatatgta tttgtttgaa gtaggcttta ttctcacttt gtttggttgt gtatttataa
      901 aactcattaa aagatcaatc gaaaaagaaa tggttattga tctaattccg acgctgagcg
      961 gctatgagta ttacgttgat tttggaaagt atgaggagtt gatccatacg gatatcgttc
     1021 acacgagtat ccatgaagta accgttaagg gcatcagatc tactttaaaa gatggacagg
     1081 agacgaaaac aatcgaccga agaaaagagg tctacaattt ttataaagtt gaagcaagtc
     1141 tacgatatgg cattgggaaa ttgaggttag atgggagtaa tattcaacaa aacaaaccat
     1201 atgttttagg aaaagtggtg atttagaact tcctatgaaa gatgaataaa taaggagaat
     1261 cttttgaaaa tgattcttct ttacagaatt gtgcttatag ttggcacgga gttaaaaatc
     1321 ttaaagaagg agcagttatt caaagtgaaa gaacatcata atcagttact atgtattaaa
     1381 acgggtacga tgaacattca ataccgaaca gaatatttag aattaacgaa tcaaactgat
     1441 tttttgtcga ttcgagagca acataagcaa gaaaaattaa tggctactta tagccaattg
     1501 attgatgagt gggaacaatt caaggaatct aaaacataaa attccattta aacaccttat
     1561 tttttaaaga gcataaggaa acgtgacccc acttttaaaa aaagtggttg gataggctgt
     1621 gcctatcacg tttctgaaaa aaataaatga ccccaaaata ccaatatata ggatagtaca
     1681 aggatagcaa aggatagtaa aaggatagag gtaggatagc gaaaaggata ggatcctttt
     1741 cactatccta tattatattg gaggatattt atgaatgtta aaattagaga tttagatcca
     1801 aaattcattt ctgaaattga tcgaagatgc gttgaactaa gtaatcgtac aggaaataaa
     1861 tggagtcgta
//
LOCUS       DACENJ010000300         1866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.313, whole genome shotgun sequence.
ACCESSION   DACENJ010000300 DACENJ010000000
VERSION     DACENJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.313"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(9..599)
                     /locus_tag="IW748_002612"
     CDS             complement(9..599)
                     /locus_tag="IW748_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1105502.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            1122..1793
                     /locus_tag="IW748_002613"
     CDS             1122..1793
                     /locus_tag="IW748_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1105503.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
BASE COUNT          656 a          309 c          321 g          580 t
ORIGIN      
        1 gagaatgatt aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg
       61 tatagacgaa tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc
      121 aagtctgtga attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca
      181 gcttgttgtt ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat
      241 tgcgcatttc cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact
      301 gccccacaaa tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat
      361 aaatgatgat tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt
      421 tcaggtattt gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc
      481 tctgattgga tagaagcagt tttgttttgc catccctctt tttggtttag tgtgctagta
      541 atataagcac cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt
      601 ttcaagctct cctttactat ctatgacaag gacaacagtt ttaacataca gacattcatt
      661 aaatttggat agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagaca
      721 gtcaacttaa ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc
      781 attataacat tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa
      841 tcttaaaaga aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg
      901 cactcagaca tgaaaacaac cctagaaata tataactagt ccaatgaaaa aacagcgaat
      961 gccgtagaaa acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat
     1021 tactgtgact aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt
     1081 ataattagta tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt
     1141 atttagatta cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa
     1201 tccctgatga tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag
     1261 aggttgcact aggtttagct gaaaattatg acaaccatgt tgtgattgat gcggactatt
     1321 ttagagcaca atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt
     1381 cttggttagt tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag
     1441 aggatacttt tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact
     1501 ttcgcgttac gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa
     1561 aacgtgaact agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta
     1621 agtccagaga gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa
     1681 tgattaagga ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat
     1741 tagtactacc caatacaata tacttcaaaa gagttggagg aggaattaca tgactgagaa
     1801 agaacaagta caacaaatcg tgaagaagta taataaaagt atcgctgatt tatcagaaaa
     1861 cgcctc
//
LOCUS       DACENJ010000301         1839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.315, whole genome shotgun sequence.
ACCESSION   DACENJ010000301 DACENJ010000000
VERSION     DACENJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.315"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(197..523)
                     /locus_tag="IW748_002614"
     CDS             complement(197..523)
                     /locus_tag="IW748_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105504.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            548..1714
                     /locus_tag="IW748_002615"
     CDS             548..1714
                     /locus_tag="IW748_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1105505.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT          648 a          324 c          277 g          590 t
ORIGIN      
        1 tagccttgct tttcaactaa gctttcaact tctttttcat tcattgtcac acctgcttct
       61 gattttattt gctataatag attcaaataa agttttctca gaactttatt attgagtgtt
      121 cccccgatct gcaaactgac gggaacactc tttttaattt tttctacttc atcaaattta
      181 aaaaacgagg tgttttttaa atttgatgac tcaatatcca ttttttgcat tcttcttttg
      241 aaaacaactc cattttttct aataaaataa ctggcaatcc cattctttta aatttcaaaa
      301 aagttgtata tgatacattc aaatatttag ccatctcttt tccttttaga aatggtcttt
      361 ctaatccggt atcaattctt gcttgatcaa tagcctcttt tgtcatttca aaaacataat
      421 ttttcaattg attcgtttgt tgtttatcta acattactgt aattcctgca tccattattt
      481 ttctcctctc attttctatt tcttcaatca gccaactcgc caagttatca gattggagag
      541 attagttatg tcaatacaaa agtacacaac aaaaagcggt gcacgatata gtgtccgctt
      601 gtatgttcgt aaagatgaaa atggaaagca agaatactat gtaaaaaaag ggtttaaaac
      661 tgaaaaagaa gccaagttac acgaagcaag aaaaaaggtc gagattgaag ataccggcta
      721 tcataagcct tctaatgact tgtttataac agtgtatgaa gaatggttgc cttcttatag
      781 gaatcgtgta caagaaacga catatcaacg tacaaaagac cttttccgct tgcatatact
      841 accaaaattc gggaataagt ccatttctaa gattactcct gttcactgtc agaatgcagt
      901 caatgaatgg gctttaaaat tcaagaatat caaacagctt aagtcttaca gctctcaaat
      961 atttgagtat gccatttttg cagagctatt acaccgaaat cctatggcaa atacaaatct
     1021 accaaagcgg gaaaagaaag aaaatgaaaa ttacttttca ttagaagaac taaaagcttt
     1081 ttgggatatt ctcaataaag aagagccttt aaaacatatt ttaatcttcc agcttttgat
     1141 tactactggt atacgtaaag gagagctagc tgctcttcat tggtctgaca tttattttga
     1201 agaacagcta atgtatgtta gaaagagcta tgcaactatt cgaaatgaag agaagaatgc
     1261 aagaagaaaa actgtcagga ttcaaaaaaa tacaaaaaat atatcttccg aacgaatatt
     1321 accaattgat gcacaaacta ttgaaatgtt aaaaatctgg aaaaaagaac aagctcgaga
     1381 gcttttgcag tttggcataa atacaaatac aaaaaatcaa cttatcttta cctatgtaaa
     1441 tgcagacggt gacattaatc aacctttgca tgcggactat tcaaataata tattgaaacg
     1501 tttagagaag aaatataaat ttaagcacgt tactattcat ggattccggc acactcacgc
     1561 tactcttcta ctagaaggtg gagcaagtat caaagaaaca caagatcgat tgggtcataa
     1621 aaatgctgaa actaccctta acacttattc tcatgttact gaaaaagctc aacgaaatgc
     1681 gttagaaaaa ttcattgctt acactggttt ataatattgt cattaccttt ctcattacca
     1741 gaacgaaaaa aagacacctt ctcgcaatga gaaagtgtct gaaatcgttg gtataacaac
     1801 gattaacgtt ttgaaaactg tgaagcttta cgtgctttc
//
LOCUS       DACENJ010000302         1801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.316, whole genome shotgun sequence.
ACCESSION   DACENJ010000302 DACENJ010000000
VERSION     DACENJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.316"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            77..1237
                     /locus_tag="IW748_002616"
     CDS             77..1237
                     /locus_tag="IW748_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1105506.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1253..1696
                     /locus_tag="IW748_002617"
     CDS             1253..1696
                     /locus_tag="IW748_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1105507.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT          629 a          286 c          363 g          523 t
ORIGIN      
        1 ggctttcatt ttctattttt ttcctgaacc catataataa aagaaagaac aaaaaaactt
       61 gcaagggagg acacatgtga agcagagtaa gcaaaaacag tctcgtcgac ttttgtttcg
      121 tttgttttta ttaatatgta tcgtcgtagg gttactgatc gctttatatc cattttatgt
      181 ggattcttta aatagtttta ttgatcaaaa acgtatcgaa caagtacaaa aacggaccgc
      241 agctgaaatt gaagtgcagc gaaagaaaat ggaggagaaa aaccaacgat taactgacca
      301 aggattcaat cctggtactg atcctttcaa tgaaaagaac agaaatgaaa gtacaacatc
      361 cagccaatta gaggaatggc tgatcggaag tatcaatata cctaagatcc aattaaatat
      421 ttcactttat gatcggctca atggtaggat ccttgaaaat ggagcaggcg tcttgcaagg
      481 aacctctttc ccgcttggtg gaaattcgac gcattcagtg atctctgctc acagcggttt
      541 acctaatcga cggttattta ccgaattaga tcgtttagaa aacggagaca cgtttatttt
      601 gactgttttg ggggagaaat tggcttatca agttaaaaat atacaagtgg tactgccaga
      661 tgacacgtct gttctaacga tttcagaagg agaagactta gttaccttgc ttacatgtac
      721 gccgtatatg atcaacacgc atcgtttgct agtgacagga catcggattc cttataacga
      781 atcggtaaaa aaagaagaag gaaaaggaaa tcaggaaaga accatgcggc aatggtttat
      841 tcttgcagga acgataatag ctgtagtcat actgttagtg tttatcggac gactaatcta
      901 tcagtataga ttaagtaaaa aagtgcttga tttttcattt attattagtg attcgtcagg
      961 gaaaccggta aatggtagtt cattcatttt gaaacataag aaaaaaacac ttactagaaa
     1021 tggtgtgccg ttttcagtac aaagcgacca tcatgggaaa gtcaagctgg atcaattgcc
     1081 tggtggaact tatcgaatcg tttcagctga tgatcctaaa gttgctgttt cttttggtat
     1141 tagaaaagta aaacaagaga agatatactt ttttgagggg cgaaaacttg taaaagagct
     1201 tcaaaaaaat ggtttttatt ttaaaatcaa tgaataagta aggagagaaa aaatgatccg
     1261 cacaacaaca aaattgtctg ccaaagaact ggaatctgtt ctatcgattt ggctgcatgc
     1321 taacttggat tctcatgaat tcattccatc aacttattgg gagaatcaag tagaatctgt
     1381 taggaaagct ttaccagatg cagtgattta cttagcagaa atagatcaag aaattattgg
     1441 ttttgctggg gtgattgatc agtttatcgc aggaatcttt gtgaaaaggg aataccggca
     1501 acaaagaatc ggtacgcaac taatgaaaaa aataaaagaa accaataaaa cactgacttt
     1561 gacagtctat caaaaaaatc aatcagctat tcatttctat ctttcacaag gttttacaat
     1621 tcaaacacag caaaaagatg aagcagtaaa tgaggaggaa cttgtgatga aatggaatca
     1681 aaacgctgat ttttgatgaa cagaataacg aaataatgct cccagcctaa ttttttgctg
     1741 ggagcattat ttcattttga agtcactaat ttaactgtgt gaatatgcag ttgttacaat
     1801 c
//
LOCUS       DACENJ010000303         1759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.317, whole genome shotgun sequence.
ACCESSION   DACENJ010000303 DACENJ010000000
VERSION     DACENJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.317"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..605
                     /locus_tag="IW748_002618"
     CDS             <1..605
                     /locus_tag="IW748_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1105508.1"
                     /translation="PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            739..>1759
                     /locus_tag="IW748_002619"
     CDS             739..>1759
                     /locus_tag="IW748_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1105509.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAA"
BASE COUNT          687 a          223 c          332 g          517 t
ORIGIN      
        1 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
       61 aaaaaggtac tgtagtagta acatatccag atggatctaa agaagaagta gaagttacta
      121 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
      181 accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca ttaccagacg
      241 gaagtcaaca ccatgggaca gctgataaaa atggtaattt tacagttaaa gttcctaaat
      301 tagaggcagg gacaaaagtg atagtaactg caactgatga atctggtaat actagtgaac
      361 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
      421 gtaagactga caatcaaggt agcaacagca atcaagacaa aaatagaggc aaaagccagt
      481 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
      541 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
      601 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
      661 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt tttatagaaa
      721 ggaaaagaat gtaattaagt ggaaaaaata gtaattattg gttcaaacca ttcagggcta
      781 gccgcagctc gatttctaaa cttaagcgat tcaaattatg aagttattct aatcgataaa
      841 aaatcagaaa ttagttactt ggaatgtggg acatcgttat ttataaaagg agaagtaagt
      901 aatttagaag atctttttta tcaaaataga atgacactaa attctgagat aaaaaagtta
      961 tttctagaga cagaagttga caaaattgat attcaaaata aaaagattag ttgtgtaaaa
     1021 aaagatggta caagattctc tcaagaatat gacaaagtta tattagctac aggttcaaag
     1081 caaattatgc tagatatacc ggggaataat ttagctaata ttttttcgat aaagaatttt
     1141 ccaaatgcaa aagagatttt taaagctttg gatgatagta ctattaaaaa agtaggaatt
     1201 attggagcag gatatattgg agttgaaatt gctgaagcca taagaaaaag agggaaagaa
     1261 gtatatcttt tcgatgttgc agatagagtg ttgtcgactt attacgaccg tagtttttca
     1321 gataaagttg aagaaatact atcaaaaaat ggaatacatc tttgtctatc agaaagtcct
     1381 aaaaaatatt taggaagaaa aaagattgaa aaaatagtca ctacaagtgg tacccaatac
     1441 tctatggata tggtcgtaca agctatagga tttctatcta atagtccaat tggggaaaag
     1501 gaactcttga gagatgttag tggagcatat ctaactaatg aataccagca aacaaatata
     1561 aaagatatat atgctattgg ggattgtgct acaacttttt ttacgtccat taatagaagt
     1621 agtgttgatt tttctatttc taatgctcta agaacagctt atattgcaac tcaacatatt
     1681 aacaatcaag attttacacc ttctggttcc caatttacaa atggcttttc tatatttaat
     1741 tacaattttt acgcagcag
//
LOCUS       DACENJ010000304         1745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.319, whole genome shotgun sequence.
ACCESSION   DACENJ010000304 DACENJ010000000
VERSION     DACENJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.319"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            378..1628
                     /locus_tag="IW748_002620"
     CDS             378..1628
                     /locus_tag="IW748_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1105510.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          578 a          334 c          307 g          526 t
ORIGIN      
        1 taatagtttt tattcaaaat gtttctagat tataaaattt attgaataac acattcctaa
       61 cggaatttgt ttcgatattg tgtgatttct cagagtacaa tggatgtatg gaaaggatcc
      121 aacgggaatt tgctatttcc tcctgtagcg tttgtctacc ctgtttagtc tgttacccga
      181 accactgtta tcactaccaa ctcattagct gtttcgataa tggactagac tttcaaatag
      241 tagtttggaa ggcagcttct agctctttta tcaccgtatt ctattaggcg aggttgtggc
      301 tatacagtga gtttctggga aatcccacag tatcgaagca ttttccaaaa tttatatcga
      361 aaggaggtcc ttcttccatg aaattatttg tcggtattga cgttagctct gaaaaactag
      421 atgtttgttt tttaacagac gacaatcaat tgtctatctt atctgaaatc tctgttgcca
      481 atgacatcga aggtgcttca ttcatacgag aaacaattct tgaattcaat aacagctacc
      541 actttgatca aattgtgatt ggcatggagt caacgtccat gtacagcttc catccttcca
      601 tgttttttca tgaagatgag aaactaaaga agctcaacac acttgtaacg attgaaaatc
      661 cttttcgggt gaaacaattt agtcgcatgt ttgatgagaa taaaactgat cgaaacgatg
      721 cattaagaat cgctgatttc ttgcgtattc aacgattcac aacttcccct atcaaagaag
      781 agaaatacat ggccttacag cgtttaacac gtactcgcta tcaacttatt aagcaattga
      841 ttcgtacaaa gcaacatttt cttgaaaatc taacgtacaa atgtaatacg cttgctcgag
      901 aaatgcgaga cgaatcgacg agtcttttta gtgcaaccct catttcactc atgacggaag
      961 atttcacatt ggatgaactt gctgagcttc ctttagaagc tttctgtgat ttacttcaag
     1021 aaaaaggaaa aggccgtttc aagcaaccag aaaaaatcgc taaagcaatt caacgtgcaa
     1081 ttaccatgag ttaccgccta ggtgctcttg cacaagaatc aattaatgtt gttttaagtg
     1141 ttctcgtacg agaaatacga gcacttgaaa aaaatatcaa agaattagat aaagccatcg
     1201 aacaagtaat tgttgtcatt ccagaatatc aatgcttaac cagtattcca ggcgttggta
     1261 aagtctatgc tgccggttta atcgctgaaa ttggtcaaat cgaaagattt gaagatcaga
     1321 caaaattggc aaaatacgct ggattaagtt ggaaagtgaa ccaatctgga aactatcagt
     1381 cgcaaaatac acctctgaca aaacaaggaa atcgatattt tagatactac ttagttgaag
     1441 ctgccaactc tgtaaaaaat tatcttcctg aatacaaagc tttttatcaa agtaaatata
     1501 aggaggttcc aaagcatcaa cacaaacgtg cactcgtcct aaccgcaaga aaatttacgc
     1561 gtctggtgga tacgctacta cgtaaacacc aactctatac gccaccaagg agtgtgatag
     1621 agaaataaca atccgttatt gacgcaaatc cttgaatcaa cccgaaaaaa atttgatttt
     1681 gatcgggtct agtttcgtgc gcttcaaaac atattactca ataaagaaaa tcttaaattt
     1741 catct
//
LOCUS       DACENJ010000305         1739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.320, whole genome shotgun sequence.
ACCESSION   DACENJ010000305 DACENJ010000000
VERSION     DACENJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.320"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..151)
                     /locus_tag="IW748_002621"
     CDS             complement(<1..151)
                     /locus_tag="IW748_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105511.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTG"
     gene            complement(295..1026)
                     /locus_tag="IW748_002622"
     CDS             complement(295..1026)
                     /locus_tag="IW748_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1105512.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(1095..>1739)
                     /locus_tag="IW748_002623"
     CDS             complement(1095..>1739)
                     /locus_tag="IW748_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1105513.1"
                     /translation="EEDIDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHF
                     RGAVRPDIRSFRELPDWIREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQL
                     HGGIATYGKDPEVQGELWDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCS
                     NPECNRQILASIENEEKHLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
BASE COUNT          482 a          408 c          293 g          556 t
ORIGIN      
        1 ttccagtcat ttcttttcgg taacgcgcga taaaaggaac agtgtttcct tcatttaaca
       61 aattaagtac agtagtcaac tgcttaggac gatagtctga aagttctttt tgtacaagct
      121 gaatgatcgt ctggttcaaa ttttccgtca ttaaaatgcc tcttttctgc taaaaattgc
      181 ttctttattc tatcatactt tttatcctgg gttaaactac tgaaagaaac aatattctct
      241 ataaaaataa aaagagccgc aacaaagtca cagctccttc attctttcca ttagtcagat
      301 tccatgccaa ggtccttcgt tgttctgata ttcaatatct tgaagaatac cttccttgtc
      361 aaaaacaacc ccatgcaatg accctccgaa tacagcccca ccgtccattc cgattttatg
      421 atcctgaatc cagaggtctg ttgtctgcat atctccatgg agcattggtg taatcgtatg
      481 tccaaataca attgtcttac ctgtattgtt tttcccttca tgaaatggtt cccttatcca
      541 aataaagtct cttagacttg tcttacgcca gtctttcttt gtcaaatcca caccggcatg
      601 gacaaaaaga taattttccc attcataata caaggggcgc tggactaaaa atttaatcag
      661 ttctgaatat ctcgattgga tcatcatcga gatttcagtt ggggagtatt cctccgatgc
      721 ccctgggtga atcaggcttt caaacgtttc ttttccacca tttctcatat aaggcgcata
      781 ccagtcctcc ggtgattgca gaaattgcaa aaaatattct tcatggtttc cccggaggta
      841 aatggctcct gtttcttcga ctaattgctt tcctaataaa aaacattctt ttgtacgagg
      901 acctcgatca ttgagatccc ctaataaaac aagctgatgg atctttggat cataatagtg
      961 aataagtttt tcaaaaagct catgtttgcc atggacatcc ccaatagcat aaatcaatga
     1021 tttcatgtgg atcaccatcc tcaacgctag tatagcgcat attggaaatg gtcgacaatc
     1081 aaaactattg ttttctattt aattaaagct gtaaaccgca gttctgcttc ttctggagag
     1141 attccatggt cttgaacata gcgattgcgc ggatgtttcg cacactctat gctacatgct
     1201 cctaaatgtt tttcttcatt ttcaatggaa gcaagaatct gacgattgca ctcaggattg
     1261 ctacaattga tatatcgttc acatggtgtt ccgtcaaacc agtcgcgacc gacgatttgt
     1321 ttatctactt ggttgatatc gacagcgatc cgttcatcaa acacatacat cttgccgtcc
     1381 cataattctc cttggacttc aggatcctta ccataagtag caataccgcc atgcagctgt
     1441 ccgacatctt tgaatccttc acggacaagc caaccagaaa acttctcgca acgaatgcca
     1501 ccagtacagt aggtcactac ccgtttttcc ataaattctt ctttgtgctc gcggatccag
     1561 tctggaagct ctcgaaaact gcgtatatct gggcgtacag cccctcgaaa atgtcccaag
     1621 tcatattcat aatcattccg tgcatcaatc accactgtct gttcatctaa aatagcttct
     1681 ttaaattctt gcggtgaaag ataggcacct gtcacttggt ttggatcaat gtcctcttc
//
LOCUS       DACENJ010000306         1702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.321, whole genome shotgun sequence.
ACCESSION   DACENJ010000306 DACENJ010000000
VERSION     DACENJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.321"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..170
                     /locus_tag="IW748_002624"
     CDS             <1..170
                     /locus_tag="IW748_002624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105514.1"
                     /translation="TGERSACSWAFTGAGLLHSGIYTAAALGLSLTGVGAAVIPFAVG
                     TAYGIAGAAIC"
     gene            671..1078
                     /locus_tag="IW748_002625"
     CDS             671..1078
                     /locus_tag="IW748_002625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105515.1"
                     /translation="MSRFFKFFSLLGVMLIGLSTLVGAGTSVYAAEKVNEVPDIETQR
                     QQAVESLKFYLEEAGYVDTETHQYIVTDLEAIQKKAELDGSEGIAGKFILENFVQPTG
                     KRQKRDLQKYAACVVMNSLPFGVLFGIYLIVKI"
     gene            1262..1617
                     /locus_tag="IW748_002626"
                     /pseudo
     CDS             1262..1617
                     /locus_tag="IW748_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          587 a          209 c          316 g          590 t
ORIGIN      
        1 aaactggtga acgaagtgcc tgcagttggg ctttcactgg ggctggactt ctacattctg
       61 ggatttatac agctgctgct ctaggtttaa gccttacggg agtaggtgca gccgttattc
      121 catttgctgt aggaacagcc tatggaatcg caggagctgc gatctgttaa tatgaaaaaa
      181 aacaatatca aatagttcat tttttaatta ctttattatt tagcccactt ttttatagaa
      241 gtttagataa atcagtagtc tttacgatgc tagttatttt acttttaaat agtacgagtt
      301 tctatttttt gaaaaactat catggtagaa aaaaactttt tcctttattt caatttattt
      361 gggatatcag tgtgatttta gtttttttct ggttatttaa atagtcactt aagaaaaagg
      421 aagaagcaaa tttgctatct tcatttgaat gatgttggta ctgtttcgct atgtaggtaa
      481 caatacaatt ttattatata ccgaaaaaat agactgtttt ttaagaagaa ttatatcgtt
      541 ctttacttga aaaaagctgt tataagacag aaagaataaa atagaaacgg aaggaacgta
      601 aaaacgtctt attgaacagc ttaaaaggat aaaaattgat aatcaacaat acttttcagg
      661 agggataaaa atgagtcgtt tttttaagtt tttttcatta ttaggtgtaa tgttaattgg
      721 attaagtacg ttagtcggtg ccggaaccag tgtttatgct gctgaaaaag tgaatgaagt
      781 tcctgatata gaaacacaaa gacaacaggc agtagagagt ttgaaattct atttagaaga
      841 agctggttat gttgatacag aaacacatca atatatagtt actgatttag aagctataca
      901 gaaaaaagct gaattagatg gctctgaagg tatagcaggg aaatttattt tagaaaattt
      961 tgtgcagcca acaggaaagc gtcaaaaacg tgatcttcaa aagtatgctg catgtgttgt
     1021 tatgaactca ttaccatttg gtgtgttgtt tgggatatac ttaatcgtga aaatctaatg
     1081 caaactttaa ttaatgcgtt gcaagggaaa aattatgatt atgctgttaa aatactttta
     1141 gatgtagcaa aaagaagttt aactcctgca caatttgcaa aattcaatgc tgcaactatt
     1201 gcagctggta tagctattaa tgctatttct tgttggggga agtaattagt ggagtgatta
     1261 tatgagtata gatgattgtt tagaagaaca aataagatta tttttaaagt ttttaaaagt
     1321 taaagttcaa aataactact tcacatgtta tagttatgca caccctgttt taaatagtct
     1381 tccttttaat tctttagtgc aaggattgat tacaaatatt gttttattag tctgtacacc
     1441 aaatgaaata tttgtaaaaa agataggcag tggtatttct tcagtaagat taaaagagaa
     1501 agaatatact caaaagatta ttcatattcc taagcctgaa attaagcgtt ttgagataaa
     1561 agactattct gctttaggac tgttctcaat tggttactgg tgataaaaaa agaatactaa
     1621 aactatacgt agaatataag taatcggcat caatcctaat tatgtgattg aatgccgatt
     1681 tttgttacgc gctattttta at
//
LOCUS       DACENJ010000307         1688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.322, whole genome shotgun sequence.
ACCESSION   DACENJ010000307 DACENJ010000000
VERSION     DACENJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.322"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(62..961)
                     /locus_tag="IW748_002627"
     CDS             complement(62..961)
                     /locus_tag="IW748_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1105516.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKKLYNQEMPQLMKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            complement(977..1570)
                     /locus_tag="IW748_002628"
     CDS             complement(977..1570)
                     /locus_tag="IW748_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1105517.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
BASE COUNT          474 a          318 c          239 g          657 t
ORIGIN      
        1 gtataacgta aatctggatc tttggttaat cgtcctacta aagttacatt attaatcatt
       61 tttatctctc catttcttga ttcatttgtt gtgatctctt aaaattttgt tttcgttcta
      121 ctttctcttg ctctacaatt gattgcatca aagaatcgac ggaagttagt gtagattcga
      181 aagaaatgtc tttggcaaaa taattttctt gttggtatct tttccacttt tccctcaacc
      241 cctcatcctt catcaattga ggcatttcct gattatacaa ttttttaaac acagattgat
      301 tatttctttc cttgaacgtt ccttgaacac tcttatacaa gtttttctca tcgatacgtt
      361 ctagttttgg aatcatatac aggtcataaa agtctttcat gcgcgaatta tcttttccga
      421 aactaaacgt agcatgtaat ttttcagcta gaatttgttc cgtaggataa gctggaatat
      481 gaatcgtttt atcttcaaac attaatttgt gttcatacat caatgccgct ggaataatag
      541 aatcacctga agtaatatct aatttaaatg gtacacgtgc attctcaaga taagcaataa
      601 actttgcttg aaaacctggg taataatcgg cttccctcgt ctctgttaat ccctttaatt
      661 caaatcgaat tccatctttt gtgggatcag ctgttaactc agaaaaaaca gtttctaact
      721 tttcttttgt tagtttcgaa tttcgataag tcgtatcaat atcaacagtt gaacggtttt
      781 ctaacccgta ttttgatccg atcagaaatc ccccttttaa tacaaaatca tcatgataag
      841 gcgattcaac aatacgttct aataattttt ccatcatata atatctttgg acaatttgtg
      901 gatttaaatg tttctctgtt gcgaaatttc tcgtttttgc tttaaattgt gtcggtgtca
      961 ttcaccttta ctcctctcaa taaagtggca acatatacaa agtcatttct tttggtgatt
     1021 ttaaaatatc catgtatctt cttagcttat ttgtatttct actggtactt gtcatatatt
     1081 cttttaatgc ttcttgtttt acctctacgc ttgctttgta tcttggattc cacatatcac
     1141 ataacgttcg ctctaaatca tagactaaaa ttttattgcc gtaaaagctt tctgtttctg
     1201 tgattcccag gtcaaaattt tctttattcg tgtaatgcgg ttgaatagca tactcgtcta
     1261 atttctcctt tgaaacattg tatcctttag gaaacatcat attgatcttt ttagggatat
     1321 acgttccgac ttgaaaaaga tcgagcgcag tctcatgaga aaaaatacct ctggaataga
     1381 tactttgagc gagatacaat tcatccacga acgcttcttt taaggtatag aaacctggtg
     1441 ctaccttttc tattttgtcc ttttcggcta atcgatctaa cgtcataggc gataagcctt
     1501 cacttctagc atccttcaag gatagattcc cattatgttt atcaaaaatg cactggattt
     1561 tgtctttcat tgcttttccc ctaaagttaa tataaaacta acataactat atcacgttag
     1621 ttttatatta acaagaaaga tatttatcga tctaattcaa tttctttttg aatccgttgc
     1681 tggttctt
//
LOCUS       DACENJ010000308         1687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.323, whole genome shotgun sequence.
ACCESSION   DACENJ010000308 DACENJ010000000
VERSION     DACENJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.323"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..450
                     /locus_tag="IW748_002629"
     CDS             <1..450
                     /locus_tag="IW748_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1105518.1"
                     /translation="EAKSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLKGDMSI
                     VGPRPERPFFVDQFNAENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIKK
                     YSLMLDLKILLQTIKILFDKMSSQGLEEDENVISLAEIAFPNEKIFR"
     gene            466..1506
                     /locus_tag="IW748_002630"
     CDS             466..1506
                     /locus_tag="IW748_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1105519.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            1525..>1687
                     /locus_tag="IW748_002631"
     CDS             1525..>1687
                     /locus_tag="IW748_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ1105520.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIER"
BASE COUNT          598 a          259 c          333 g          497 t
ORIGIN      
        1 gaagcaaaat ctggaccagt tttagctaag agcaatgatg cacgagtaac gcctgtaggt
       61 aaatttattc gagctgtccg ctttgatgaa ttaccacaaa tcttcaatgt gctgaaaggc
      121 gatatgtcga tcgtaggacc acgtcctgaa cgtcctttct tcgtcgatca gttcaatgca
      181 gaaaaccctt attactactt acggcataat gttcgtgctg gtatcactgg gtatgcacaa
      241 gtctatggaa aatatgtctc tgattacaac agtaaattac gttttgattt actttatatc
      301 aaaaaatatt cattgatgct tgatttaaag atcttgctgc aaaccatcaa gatcctattt
      361 gataaaatgt cctctcaagg acttgaagaa gacgaaaatg tgatctcatt ggcagaaatt
      421 gcttttccga atgaaaaaat ctttcgctag ttgggaggcg taaaaatgaa caatccgaaa
      481 ttcagcataa tcgtaccagt atacaatgca gaagcaacta ttggtcgaac gattgatatt
      541 ctaaaagaat tggattatga agagtacgaa gtcgttttag taaatgatgg atcaacagat
      601 caaacacagg aaatcattca agaactgatt gaaaatgatg aacgttttga attgattact
      661 acgaccaatc aagggcccgg attagcaaga aacgaaggaa taagacagtc aaaaggagaa
      721 tatctcttat tcttcgatgc agatgacacg ccagtaaaaa cgatcttgtc agattatgat
      781 catttgctag aaaagagtcc aaaagcggat cttgttgtta gttcgtttgt ttttcgtaca
      841 atggacgagg gaagaatcgt ttccgaaaaa gaaaacttag ttacagaatg tcactatcta
      901 actcacgaag agtttataaa agacatgtat cagttaatga atgaacagtt gatgtatgtt
      961 gtatggaaca aatgctatcg tagcgatatt ctgaaaaata accaaattct atttaaaact
     1021 tataatagtt gtgaggatcg aatatttaac ttacattatt atgaatactg tcatcaagtg
     1081 atcatgaacc caaaagtaga atatatttat gaatttgaag gtgggaaggg gatcactaat
     1141 caataccgac ctaacaagtt cgaaacattt aaagaattct attcattagc aaatactgta
     1201 acagacgata ttaataaaga cggtatggcg gcgcttcttt taaaagggac aacttcagtt
     1261 attttttcta tttatagtac ctctgcaaga acttcaaaag agaagaaaga cgaagcaaag
     1321 cagatattga gtgatccagt gatccgagaa gccaagcaaa ttgctaagac ggattcttca
     1381 gcaaaaaaaa taacaaagtt gctttacaat atgccagctc cactattttt atctgctgta
     1441 aaagttggaa gcttcgttga agtgaaaatg ccagggttaa tggctgcttt gaagcgagta
     1501 tattagttaa tggaggaaaa tataatgaaa agaattttgc attttcaggg acgaatgggt
     1561 ttaggtggag cagagtcttt catgatgaat ttatatcgta aaattgatcg tacaaactat
     1621 caatttgatt ttttgattta tgaagattat gcagatgttc aagactatca ttcagagatt
     1681 gaaagat
//
LOCUS       DACENJ010000309         1669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.324, whole genome shotgun sequence.
ACCESSION   DACENJ010000309 DACENJ010000000
VERSION     DACENJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.324"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW748_002632"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW748_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1105521.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT          513 a          304 c          318 g          534 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggtta
//
LOCUS       DACENJ010000310         1633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.325, whole genome shotgun sequence.
ACCESSION   DACENJ010000310 DACENJ010000000
VERSION     DACENJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.325"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            143..1627
                     /gene="guaB"
                     /locus_tag="IW748_002633"
     CDS             143..1627
                     /gene="guaB"
                     /locus_tag="IW748_002633"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1105522.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          561 a          283 c          378 g          411 t
ORIGIN      
        1 atctctttta atatacacag atgtaaacgc tttatattgg tattctatga gaaagctaac
       61 ttgattccaa gtagatcatt gacttaaatg aaaaaatcaa ttattttaat agataaacta
      121 aattagagga gtggtactaa aaatgtccaa ctgggaaaca aagttcacga aaaaaggatt
      181 tacttttgat gatgtcttat tgataccagc agaaagtcac gtgttaccaa acgaagtgga
      241 tatgagcgta caattagcta aaaatattaa gttgaatatt ccaatcatca gtgcaagtat
      301 ggatactgtt accgacagca agatggccat tgctatggca agacaaggcg gactaggtgt
      361 gatccataaa aacatgacga tcagccagca agcggatgaa gtaagaaaag tcaaacgttc
      421 tgaaagtggc gtaatcattg atccgttctt cttaacgcca cagcatcttg tagcagacgc
      481 agaagaattg atgagcaaat atcgtatcag tggtgtgcca atcgttgaaa cacttgaaaa
      541 cagaaaattg gtagggataa ttacaaaccg cgacatgcgt tttgtaacag attatcacat
      601 gccgattgct gatgtgatga caaaagacaa cttagtgact gccccagtag gtacctctct
      661 aaaagatgca gaaaagattt tgcaaaaaca taaaatcgaa aaattaccaa tcgtagataa
      721 cgaaggtcgt ttaagcggat tgatcacgat caaagacatc gagaaagtga tcgaatttcc
      781 aaatgcagca aaagatgaac atggtcgatt gcttgtagca gcagccgttg gtgtaacaag
      841 cgatacattt gagcgtgcga atgctttatt agaagcaggg gcagatgcaa tcatcatcga
      901 tacagctcat ggacatagtg caggtgtgat ccgaaaaatt caagaaatcc gttcaacttt
      961 tgcagatgca acattgattg caggaaacgt tgcgacggct gaagcgacaa aagctttata
     1021 tgatgcaggt gtagatgtcg taaaagttgg tatcggacca ggatcgattt gtacgacacg
     1081 tgtggtagcc ggtgtaggtg tacctcagct cacagctatt tatgatgcag catcagttgc
     1141 tcgtcaatat ggcaaagcga ttattgcaga cggagggatc aaatattctg gtgacatcgt
     1201 caaagcatta gctgccggcg gacatgcagt gatgctagga agtatgttgg cagggacaga
     1261 tgaatcacca ggtgaattcg aaatcttcca aggacgtcga ttcaaaacat accgaggtat
     1321 gggctcatta ggcgcgatgg aaaaaggttc aaaagaccgt tacttccaag gtagtgtcaa
     1381 tgaagcaaat aaattagtac cagaaggaat cgaaggtcgg gttgcttata aaggaagtgt
     1441 tgcagatatt attttccaaa tgatcggcgg tttgcgttca ggaatgggtt atgttggggc
     1501 agccaatcta cagcaattac gtgatgaagc gcaatttatc caaatgagcg gcagtggatt
     1561 aaaagaatca caccctcatg atgtacaaat cacaaaagaa gcaccaaact attcagttga
     1621 atcttaaaaa cat
//
LOCUS       DACENJ010000311         1607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.327, whole genome shotgun sequence.
ACCESSION   DACENJ010000311 DACENJ010000000
VERSION     DACENJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.327"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            31..468
                     /locus_tag="IW748_002634"
     CDS             31..468
                     /locus_tag="IW748_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105523.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            461..1168
                     /locus_tag="IW748_002635"
     CDS             461..1168
                     /locus_tag="IW748_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105524.1"
                     /translation="MNNVVYQLTMEDISRFLTNKEHLNEVIRRIQKDFSCMEEMKYFV
                     QNVEEQLYLESETSQTKVTVSNNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKS
                     ELVENFISYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGIDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKQMPYQRFERKKGQ"
BASE COUNT          598 a          240 c          330 g          439 t
ORIGIN      
        1 ttttgattga agagaaagga agataaaatg atggctcaaa attactatga cgaatttgtc
       61 aaattacctt tggataaaat ggcacaaaaa atggaggata tgacgttcct atacaacgaa
      121 acacgtgttc caaaaaaaca ttataaagaa aaattgtcgg tagctgtgga agaaatgatt
      181 gaatcaggtg tcgaaatgaa tcttattgcg acgtactatc gaacactgga agaactgaaa
      241 aagcaaaatg caaaatggtt tttccaagcc ttactatgct tggaagtagg tgtcaaacca
      301 agcactatta aaccttctga ataccaagct ttagaactaa cgtatgcgaa atttatcgaa
      361 acgaaaaaag ccaagactgt ttcaagtgaa tggttagatt attttgaaaa tatcaataag
      421 tatggcgcct attacaccat gaaaaaggag gacaacgaaa atgaataatg tagtttatca
      481 actaacaatg gaagacattt ctcgtttttt aacaaacaaa gaacatttga atgaagtaat
      541 cagacggatt caaaaagact ttagttgtat ggaagaaatg aagtactttg ttcaaaacgt
      601 tgaagaacag ttatatttgg aaagcgaaac gagtcaaaca aaggtcacag tcagcaataa
      661 tcgtttagat gtgtataaac gtcgtcctga atcattaaaa aaaggagcgg agcttctacg
      721 actagaaaca gaaaaatacg tcatgaatgt ttacctagaa aaaagtgagt tggtcgaaaa
      781 ttttattagc tacacgattt gcaggaaaca ctattactca gaatcagaat attattcaat
      841 ttttgagagc atggaattaa tggaagaaga aatggaagag ttaaacagta aagatgcgac
      901 ggtagacgat tattaccttg tatttactag atttttaacg tgtggaacgg aatctatcgt
      961 gctaatggga atagattacg aactcgctgt acccgaagcg tttttgaggg aatatgtaga
     1021 agaagtagat aacccagggg atgattggta cacgacttta actgattttt tagacaccta
     1081 cacttacgac gatacagaag aaatccaaaa tgagcttgac cgtaaacaaa tgccatatca
     1141 acgatttgaa aggaaaaaag gtcaataatc atttataccg aaaagtgaga atgtgtgaaa
     1201 taagaacaaa aaaccttgaa attaagggaa tggttcttat ttcgcaccaa tcgctccaac
     1261 cgcccccgcc gcttaggtgg tgcgaaataa gcgagcaaag cccgctaacc aaagagaaag
     1321 aaggagattt atatgaaaaa gattattccc ttattattag tactatttgt tggcgaaatg
     1381 cttatgcgtc atgctgcaac tgtgcattta aacttcgtag tatttttggt aatactggtg
     1441 ttttgtttga ccttttggcg gtttgtatta gccctcgtga gtttactatt caagatgatt
     1501 gcagccatat ttttcatagg attatttcga tgagaggaga gagtatcaat gccaattgca
     1561 ataggaaata aacggttacc cgttacgttg gatgaaaaga gacaaaa
//
LOCUS       DACENJ010000312         1600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.328, whole genome shotgun sequence.
ACCESSION   DACENJ010000312 DACENJ010000000
VERSION     DACENJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.328"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            25..414
                     /locus_tag="IW748_002636"
     CDS             25..414
                     /locus_tag="IW748_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HAQ1105525.1"
                     /translation="MSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIA
                     VENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTI
                     AFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            542..1225
                     /locus_tag="IW748_002637"
     CDS             542..1225
                     /locus_tag="IW748_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1105526.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1542..1600
                     /locus_tag="IW748_002638"
     tRNA            1542..1600
                     /locus_tag="IW748_002638"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1568..1570,aa:Arg,seq:tct)
BASE COUNT          533 a          261 c          339 g          467 t
ORIGIN      
        1 ccttatatta ttgaggacaa agaaatgagt ccatattatg cacaaggaaa tctgatatat
       61 gtacgaccga aaaatacaaa tgagatttta gtcggagata ttattacata ttatgaaaat
      121 tcaggaaaaa aaatctcgac gcgtcgcgta atagccgttg aaaacaacca cgaagatttt
      181 tatacaaaag ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt
      241 atcggtagtc ccatctttca gattccatat attggactag tcctttcaaa aacggcattt
      301 gcatggattc aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg
      361 aatacctttg tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt
      421 taaaaacata aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa
      481 cactcatgca gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag
      541 tatgcgcaat gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg
      601 tacccagcaa aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc
      661 agaagtacca gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct
      721 tcagcctgaa cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc
      781 cacgacaatc ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga
      841 gaaagctaaa aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg
      901 tcaagcagca gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag
      961 tgcaaaatca aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg
     1021 cgttttgatg gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat
     1081 tccccgtggc aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga
     1141 tcatcccgat ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa
     1201 ggaatcagaa acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga
     1261 atcgagggct caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga
     1321 tacaggaaat ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg
     1381 ataaccttag aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg
     1441 ccaaaataat tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa
     1501 ttatctataa ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca
     1561 tccgccttct aagcggacgg tcgggggttc gaatccctcc
//
LOCUS       DACENJ010000313         1576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.329, whole genome shotgun sequence.
ACCESSION   DACENJ010000313 DACENJ010000000
VERSION     DACENJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.329"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(172..1464)
                     /locus_tag="IW748_002639"
     CDS             complement(172..1464)
                     /locus_tag="IW748_002639"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1105527.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          440 a          342 c          277 g          517 t
ORIGIN      
        1 gccgtagatg caagcttttg aacaccgttt gttcggaaaa agggactgtg acacggtttt
       61 tgtcacagtc ccttattctg aataaacagt gttcaagtta ttattatcgt atagataaac
      121 acttcgactc gctctggtaa gaacgtcctg attcagtcgt cgtttttatc cttagatttg
      181 cgcccagacg ctttctaaga tgtttgtttg tgttctatca ggtccgacag aaaatgttga
      241 gatacgtaca ccaacaagct ctgagatacg acgtacatag ttacgtgcgt tttctggcag
      301 ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag cctggcaatt cttcatagat
      361 tggtttgcag cggttcaact ctttcaagct tgctggatag tggtagatta gttcgccatc
      421 taattcataa gccgtacaaa tttttactgt ttccaatcca cttaatacat cgatcgagtt
      481 taaagatagg ttggtaatac ctgaaactcg tttagaatgg cgcataacta cagaatcaaa
      541 ccagcctaca cgacgcggac gaccagttgt cgtaccgtat tctcgtccta cttcgcggat
      601 ttgttctcct gtttcatcaa ataattcagt tggaaatggt ccatcaccga cacgtgacgt
      661 ataggctttg cagacgccga caactttatt gatttttgaa ggccctacac cgcttccgat
      721 tgttacccca ccggctactg ggttagaaga agtaacaaaa ggataagttc cctggtcgat
      781 atccagcatt acaccttgtg cgccttcaaa caacacgcgt ttgcctgcat ccaaagcatc
      841 attcaagata acggaagtat ctgtgacgta ttgtttgatt tgttgtccgt attcataata
      901 ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca aacattttta caaattgacg
      961 gtttttgtca tctagattca aacgaagacg ttcttcaaaa atttctttat ctaataagtc
     1021 tgcaatacgg atacctacac gagctgcttt atccatatag gcaggtccga ttcctttaat
     1081 cgttgtacca atcttatttt cgcccttagc atcttcttgt aattggtcca atttgatatg
     1141 ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa ttacttgttt ctacaccttt
     1201 ttcatgtaaa taagctaatt ctttaaccaa tgatttagga ttgactacaa cgccgtttcc
     1261 gatcacactg atcttgtctt tataaaaaat cccagaagga atcaaatgca gtttataagt
     1321 cacaccgtta aacttaattg tgtgccctgc attgtcgcca ccttggtacc gtgcaatcac
     1381 ttcagcattt tcactgagaa agtctgtgat ttttcctttc ccttcatcgc cccattgcgt
     1441 acctactact actacagatg acatgtgaac acctcgttct atattattta atcctgaacc
     1501 attgtatcaa taatagatac atatttcaaa taaaatcgaa tgatttttat ttttatttta
     1561 taaaaacgct taaaaa
//
LOCUS       DACENJ010000314         1549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.330, whole genome shotgun sequence.
ACCESSION   DACENJ010000314 DACENJ010000000
VERSION     DACENJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.330"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..377
                     /locus_tag="IW748_002640"
     CDS             <1..377
                     /locus_tag="IW748_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105528.1"
                     /translation="LGLIQGSGFVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLL
                     YFSCLLVFTSMFSLLPRLIIGPNGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILAT
                     FFYFWTEIRIKSERSVSIKNNY"
     gene            574..1464
                     /gene="pstS"
                     /locus_tag="IW748_002641"
     CDS             574..1464
                     /gene="pstS"
                     /locus_tag="IW748_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1105529.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          522 a          273 c          319 g          435 t
ORIGIN      
        1 tattaggcct gatccaagga agcggatttg tggtttctag gaattttcca gctttgattg
       61 ttttctattt agtgataagt gtaggtctct tattaccaaa atctctgatc aatcactaca
      121 attggcagct gctttacttt tcttgcttat tagtatttac atctatgttt tctttgctgc
      181 ctcgcttgat tatcggccct aatggtttga gcgagagtgg attgaaggat ttagcttatt
      241 cctccatgta tttcttgacg atcaaaccat ggttctattt agtaattgcc tcatttattc
      301 ttgcaacatt tttttacttc tggacagaaa tcaggataaa aagtgaacga tctgtatcta
      361 taaaaaataa ctattgatgg tggatcatca gtaaaaaaac atatgagctg cgtaaaatgc
      421 atgtttctca tatgcttttt ttattattat gtaaagaatg cgaaaaacgt ttacatgatt
      481 tttacataac ttaactatat ttttacagca accatacgag ggatttacag tcgactgata
      541 aactttagtt gtaaataaga aaggagcaat aaaatgaaaa aagtattgtt tactctaggt
      601 atgatgggaa ttcttttagc cggatgcggc tcaggatcag gaaatacaga tggctctgcc
      661 acacaaaata attcaagtga ctcaaatgaa caagtaaaaa tcgttgcggt aggttcgaca
      721 gcattacagc cgttagtcga tgcagcacaa gaaagctttg tacaagaaaa tccaaattat
      781 caaatctctg tacaaggagg aggaagcgga actggtttga gccaagtcga agctggagca
      841 gttactatcg gaaactctga tgtatttgca gaagaaagag acggagttga tgcttcaaaa
      901 ttagtggatc ataaagtagc agtggtcggg atggcaccaa tcgtcaataa agatacagat
      961 gtcaaagata tcaccaaaca ggaattgatt gatatcttta caggaaaaat cactaactgg
     1021 aaggaagttg gcgggaaaga tcaaaaaatc aacgtcgtga accgtgcaaa cggcagcggt
     1081 acccgcgcga cattcgaaaa atggggactg gatggtgcta cccctgtgca gtcccaagaa
     1141 caagattcat caggaacagt tcggcagctg gtaagtcaaa caccaggagc aatcagctat
     1201 ttagctttct catatctcga tgattccaca caagcactaa gtatcgacgg tgtagaacca
     1261 aaagaagaga atgtagcaga taacagctgg ggaatctggt catatgaaca tatgtacacg
     1321 aacggaaaac cttcccctga agtccaaaaa ttcttagact acatgatgac agaagaaatc
     1381 caagaaggac ctgtcaaaga attgggctat ttaccaatca caatgatgga agtagaacgt
     1441 gatcacgaag gaaatatcaa ataactagca gataaacatg ccacggtagt cgttactatt
     1501 aaggaaaaat caactatgtt tccccacata tacatttttc ctttaattg
//
LOCUS       DACENJ010000315         1530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.332, whole genome shotgun sequence.
ACCESSION   DACENJ010000315 DACENJ010000000
VERSION     DACENJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.332"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(31..579)
                     /locus_tag="IW748_002642"
     CDS             complement(31..579)
                     /locus_tag="IW748_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105530.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(580..1437)
                     /locus_tag="IW748_002643"
     CDS             complement(580..1437)
                     /locus_tag="IW748_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1105531.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          407 a          318 c          215 g          590 t
ORIGIN      
        1 aaaggtagtt acttagttct tctttgatta ttatctaact ttattattca ttccccaccc
       61 tttgcgcagt gagtcttcgg tatcttgttt tatctgactg gtagttctta ttatttctcc
      121 acgatcatct aattctggaa caattggata tttttctctg aacaagtcta gttctttcac
      181 cacgtcatta aacaaatctt gtttatcttc ttgcgtaaga ttctgtttaa tatactcatc
      241 taaagctttt ctaagcaatt catctcttgt catattagtc tcatactttt ctactaaaac
      301 tctggaagtc gcatttaaca aagtcgacag ttttaaagga tgcttggtgg aataaacctt
      361 atcaaacttt ctgattggtg cgccaacttt ttttgttctt cctctactct gtctttcaac
      421 attggaggaa gtttttattt cttgaacttg gtgcacttct aattgcttat tttcagatgg
      481 tgtaattgct tgctgctttt tctttgcttc tgcttctttt tttattttgg cgagtccttt
      541 gttgttccca aaaatatcaa gtcctttgat gtcagccaat tactccacct ccgcatacca
      601 attctcgtgt tcttccaatt ctttcaaaat actgataaat aattcgtggc ttttgcgatc
      661 ccattgtttt aagagacctt tctttgtctt ctccatcgta atacctgatc cgtcgtatcg
      721 ttttaagcgc gcatttttga gtacaacatt tttaatcaaa tgttccccat aaatctcttg
      781 ggcttgttta tacatttcct gatctactgc gtcaccgctt tccaacatga aaggaataac
      841 accagcaaca tataaatttg tatcaaattc tttcacaaaa aagtccatat actcaagata
      901 ttgcatcgca tttctcatcg attttacttg ggtctgtaag acgacgattg cccaatccgc
      961 aatgtacatc gcggatttac tatatacaga aatagaaggt gggacatcta aatagatgac
     1021 gtcaaactca tcaaataatg gttctagctt ttctttcata tatttgaatt gggataatga
     1081 atcattatat tggtctttga tccaatcatc atacatttca aaggacatgt ctgctggaag
     1141 tatatagagg ttattgatta ctttatagac agcctcttta ggtggaagat tgtttatcaa
     1201 cacgtcagtc acagtatttt cgtaaacaat cggttcacct tctccgcctc cttcttcgtt
     1261 agtgaagttc atatgcgtta agttcatcac atcagaagta tttccttgca tatccaagtc
     1321 aattatcaag gttctttttc cttcattaac tgctgattca aaccctaaca tttgcgcgca
     1381 cgtactttta cctacgccac ctttaaaatt accgattaca tatttcttcg ctaccacgtt
     1441 cctacctctt ttcttttagc aatattcatt tgatactatt ataacatatt tatttttagt
     1501 tactaccaaa aaaaagatat atatgtacta
//
LOCUS       DACENJ010000316         1525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.333, whole genome shotgun sequence.
ACCESSION   DACENJ010000316 DACENJ010000000
VERSION     DACENJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.333"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            12..1478
                     /locus_tag="IW748_002644"
     CDS             12..1478
                     /locus_tag="IW748_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1105532.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          324 g          495 t
ORIGIN      
        1 gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg aagggattgt
       61 caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg cgtgcattac
      121 ttatctatta tatgtacgat acagttgcca acgggggact tggattacca cgagcaacag
      181 cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc gtcggcggat
      241 gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga gttttcatta
      301 tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt gtttcgatgg
      361 cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg gtcggacatc
      421 tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac atgggtgtca
      481 acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa tataactatc
      541 atttagggtt ctctttagct gctattggga tgttctttgg attattacaa tacatcatcc
      601 aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg acaactgaag
      661 aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct gcagtattcg
      721 gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca atcagtgtat
      781 tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa gatgtaactg
      841 ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca atcattttct
      901 ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa cgtacacaat
      961 ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat ccaattttca
     1021 tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa cgtcaaccat
     1081 ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac ttgctattga
     1141 tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg ttgttgggaa
     1201 gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg tctgtgacaa
     1261 caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc ttagctgatg
     1321 cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga acagaatctg
     1381 cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta tttactgtga
     1441 aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata aaaacaggaa
     1501 ttgcttattt gcagcagttc ctgtt
//
LOCUS       DACENJ010000317         1516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.334, whole genome shotgun sequence.
ACCESSION   DACENJ010000317 DACENJ010000000
VERSION     DACENJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.334"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1360
                     /locus_tag="IW748_002645"
     CDS             <1..1360
                     /locus_tag="IW748_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HAQ1105533.1"
                     /translation="VIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNHDILWIG
                     AYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTPKNRKDL
                     SEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIVLEGQLY
                     ELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGMYLTNNQ
                     HVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLATDLIWY
                     AWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAEFDAKEE
                     GSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNSYGFQIV
                     THYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQRLLDYRK
                     DD"
BASE COUNT          518 a          277 c          313 g          408 t
ORIGIN      
        1 tgtcatcgat catttgcata tcgtgggtga tatttttgat cgaggaactc aagcagccaa
       61 agtaatggat cgaatcatgg atttttcctc tgtagatatc cagtggggga atcatgatat
      121 tttgtggatt ggtgcgtatt gcggctctga ggcatgttta ttgacgcttt tacggattgc
      181 cgcacgctac aactatttat ttgaattaga gaaagaatat ggattaaatt tacgtccatt
      241 attttctttt gcccatcaaa cctatcagaa gaatccagta tttaccccta aaaacaggaa
      301 agatctatct gaaagagagc aagaagaatt agaaaagatc catcaagcac tttctatcct
      361 ccaattcaaa tcagaacatc agttgttgaa tcgacgacct gaatttaaaa tggacgatcg
      421 taaattgttg gaaaaaatca actatgagga atcaacgatc gttttagaag gtcagctcta
      481 tgagttgaaa gggacttgtt ttcaaacgat ccagccagat gatcctaaaa aattcttgga
      541 agaggaacaa aaagtgatcg atagtttgat gtattcattc cagcactcgt taagaatgca
      601 aaaacacatg acgtttttga tcgaaaaagg gggaatgtat ctcacgaaca atcagcatgt
      661 actgttccat ggctgtatac ctgtagatga aaaggggcag ctcttagcat ttgaattaga
      721 ccaatcttat cgcggaaaag aactgctggg attttttgaa aaacagataa ctgaaagcgc
      781 aaaagatttg actgcaaatg aagatttggc gaccgatttg atttggtatg cgtggagcgg
      841 gccattttcg cctttattcg gcaagagtaa aatggcaact tttgagcgct attttctcac
      901 agagcctcat acgcacgtcg aaaaaagcaa tccctattat cacttgcgag atgaagagtg
      961 gttcagtgaa aaaattttgg cagaattcga tgcaaaagaa gaaggttcag caatcatcaa
     1021 tggacacacg ccagtaaaag taaaaaaagg agagtcaccg atcaaagcaa acggaaaagc
     1081 atttgttatt gacggaggtc tatccaaagc ttatcaaaaa acaaccggga ttgctggcta
     1141 ctcattacta tgcaattctt acggtttcca aatcgtgaca cattatccgt tcttgcctat
     1201 tgaacaacaa tttgcaaaaa aatctgatca aacgaatgta aaaaaagtga tcgagcagca
     1261 gctcccacgc aaacttaatc ggaatacgac aaaaggaatg gaattacagc aacagattac
     1321 tcaactccaa aggctgttgg attatcgaaa ggacgattga gaaaaatgct agccaacatt
     1381 gctcaataga ctaaaaagcg actgcaacaa aaggcgtgtc gcagtctcct tcggaattag
     1441 ctcaaaaaac ggtaaaatat tagaagctat ttttctgttt atttctctaa tactcaggtc
     1501 agggcgctga tttcac
//
LOCUS       DACENJ010000318         1497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.335, whole genome shotgun sequence.
ACCESSION   DACENJ010000318 DACENJ010000000
VERSION     DACENJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.335"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..656)
                     /locus_tag="IW748_002646"
     CDS             complement(<1..656)
                     /locus_tag="IW748_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter solute-binding protein"
                     /protein_id="HAQ1105534.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     S"
     gene            complement(653..1495)
                     /locus_tag="IW748_002647"
     CDS             complement(653..1495)
                     /locus_tag="IW748_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1105535.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
BASE COUNT          524 a          281 c          182 g          510 t
ORIGIN      
        1 gagaaatatt taaagcatcc ttcacttgta acaatcattt ttttatcatt cggaattgaa
       61 gcaaatttag atttagcttc tttgtctaga gaatccaatt tttcaatata cttatctaga
      121 ttttctttat agaatttttt attatcagga tctttttccg ctaactgttt ttcaatattt
      181 ttagcgtaaa taataccgtt ttctaaattt aaccaagcat ggggatcttc cttccctttc
      241 tcactctgac cctctaagta aataacatct atgccatcac ttgctgcaaa atagtctttg
      301 ttttcctctt tgttcgcatt ttttactaat tttgtaaacc aagcatttcc tccagtttcc
      361 aagttaacac cgttataaaa aatcaaatct gcttttgaag ttttttgaac atcttcaggc
      421 aaaggttcat attcgtgggg atctttccca ataggtacga tactgtgtaa atcaatttta
      481 tcttttgcta tattttcagt aatatctgct aaaatcgaat tagtaactac tacctttaat
      541 ttttcgttcg aagccactgt atctttgtct ttactatttg ttgaacatgc cccgaatata
      601 aaaagagcaa tcaaaatccc cagacttccc agtactgctt tccattttct catcataact
      661 ttctctcctt ctttcttaaa aatttttgtt ttggtgagat tagaaaacta attacaaata
      721 agaatgccgc tgttaaaaca atacttgatc ctgctgctat gttaaaacta taaccaatga
      781 acaaacctag caatgatgag cacgctccga ctgtcgccga aataaatatc atagttttta
      841 agctgtttgc atataaatat gctgtagcag ctggtgtaat taacattgca acaattaata
      901 tagttcccac actttgcata gctgttactg ctactaaagt taatagaatc attaatagat
      961 aatgataaaa actgacattc atccccattg cttttgccat taatggatca aaagaagtga
     1021 ttaataattc tttaaaaaat agaacaactg taaccaacac taaaacagaa acgccaattg
     1081 taatccactt atcaatatct tgtacagcta aaatatttcc aaataagata tgaaataaat
     1141 ccgttgagct atttgcaact cctataagga taactcctaa tgctaaaaaa gaactgaaag
     1201 taataccaat tgctgtatct ccttttatta cactattgct ttttataaaa gtaataatga
     1261 tagaagctaa tagaccaaat acaatcgcac caataaaaaa attaatacct aaaatataag
     1321 aaattgctac tcctggcaaa acagcatgag aaatagcatc acccataaga gacattcctc
     1381 ttaaaataat aaaacaacct actgttccag aaacaatccc tataactaca gatgtaatta
     1441 gagcattctg taaaaagtga aagtcataca gaccattaat aaaattttga agcatat
//
LOCUS       DACENJ010000319         1475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.336, whole genome shotgun sequence.
ACCESSION   DACENJ010000319 DACENJ010000000
VERSION     DACENJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.336"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            53..>1475
                     /locus_tag="IW748_002648"
     CDS             53..>1475
                     /locus_tag="IW748_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1105536.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQ"
BASE COUNT          502 a          250 c          301 g          422 t
ORIGIN      
        1 ctcatgacct ttttatgacg tactggtata cttgaagaaa aaggagagcg cgatggagat
       61 aaaaaaagtt tacttttcac agaaaatgtc gctgtattcg cctatccagc aagcaaaaca
      121 accccttcca ttttttcaaa gtcatcaaga aagtagagac gctttttggc gttctgcaaa
      181 aaagcaatgt gcttttaaag cagtatcgaa aaaatatacc gtctatttta ctattgctaa
      241 aagtagagga gatgttattt ttaataatct gttgattgaa ggtgaaactt ttgaatggaa
      301 aaaatttttt cggtatatgg aagcttgcgc tcgttttttt attaaagaaa actgttgttt
      361 tttatttcaa tctcctctat cagaagaatg gttgcgtatt tttcataaaa acggctatca
      421 aggaaccaca caattaaata aaaagctggt ctatcataca gcacttgttt taggtggagg
      481 aggagcacac ggtgcttatc agattggtgt gtggcaggcg ctgaaggaac atgatatcaa
      541 ctttgagatt atcacaggta cctcggtagg tgcgttgaac ggcgcattga ttttacaagg
      601 agatatgaaa aaagctgtga atttatggaa aaagctgtcg acaagacaag tgcttgcgtt
      661 accagaaatg gctgcagttg aagaccttcg tgaacgcttc tatcgagaaa gacgccaaat
      721 gacgaaaaca gcactaattg aaggcggggt ttcttctgct ccattggaaa agatggtcaa
      781 agacaatttg gatttgtcct tactcaaaca taatcccaaa atacgtttat atacggtgtc
      841 tactaaactg ccagaactag cggaagtagt gactgatatc caacagacaa aaacagaaga
      901 aataccagat tggatactcg cctctgcttc attttatccc gctatggctt atcgaaagat
      961 cggaaaaaat aaatatgcag acggtgggta tcgcaacaaa atacctattg atattgcgat
     1021 taacaaagga gcaacagagg catttgtcgt agatgtgcaa gggccaggcc cagcgaaaaa
     1081 aattcgtatg ccagacattt ttatccattg gaaatgtcaa acattgtgga cactaggcag
     1141 ctttctgctt tttgatagcc aaaggaacca attaaattta caattaggct atctggaaat
     1201 gaaaaaacga ttaggctatt actatgggaa ctggtataca tttcattctg ttaaacctgc
     1261 aagaacatac tggcgaggat ttttgtctta cttaacaaaa gaaattcagt tagaattatc
     1321 ttttttcaaa agcataaaat tttggcaaaa actgcgtaat ttatataaga acagggtcgt
     1381 accagagaca tgcggattgg cgatgctaga acttttagca aaaaaacatt ttttactgcc
     1441 caatgaaatc tatcaagtag acatgatgat tcaaa
//
LOCUS       DACENJ010000320         1425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.340, whole genome shotgun sequence.
ACCESSION   DACENJ010000320 DACENJ010000000
VERSION     DACENJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.340"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..279)
                     /locus_tag="IW748_002649"
     CDS             complement(<1..279)
                     /locus_tag="IW748_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1105537.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLY"
     gene            complement(391..1389)
                     /locus_tag="IW748_002650"
     CDS             complement(391..1389)
                     /locus_tag="IW748_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1105538.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
BASE COUNT          424 a          284 c          248 g          469 t
ORIGIN      
        1 gtaaaggcct tcgtcatctt cgataatatg ttgcaacgga ctcatcaatt ttttcttttc
       61 tgctaatata tctaattgga ttccagtgat aatggcgtta tgtacttcac gctttgttag
      121 cacgctgttg acactttcag tacaatccgc caaagtcaaa tcagaaatat agtctttttg
      181 taaaaacatg acaagttgtg cgatatcttc tacagttacg ccacgttcct tcaatagttc
      241 tcttgccttt tcttctaaag tctcagtttt gacgaccata ttattgctcc ccctttattt
      301 tttctttctc tatttcatgt taacggaaca tgaaaaaaat gcaagaaact gctttcgggt
      361 ttaacttgaa atgcaaagag atataaaaaa tcaactagga cgaagatttt tatcgtataa
      421 gcttgtgctg tgaccaggct gagttcgttc ctttggatta atatagtgta aagcgttgtt
      481 cactgcagta ggtgcttcac cgagcccagt agcaatcagt ttcacttttc cttcataggt
      541 acagatgtca ccagctgcat aaacgcctgg aatagaagta gacatatctg attttacagt
      601 aatggcattt ctggtactat caagtcccca agaagaaaga tgttctaaat tagaagagaa
      661 tccataattg acgatcagag aatcaatcat caaatcaatc gtctcgtcac ttttcacttt
      721 ttttaaccgg atatctgtca gttccccatt attgcctgat aatccatcga tgagataggg
      781 agtaagcaag ttgacagatg aagatttcaa gcgtgataca ctatgttcgt gcgcacgaaa
      841 ttccgggcga cgatgaatca agtaaacttc gcttgcaata ggctctaaca tcaacgtcca
      901 atcgatagca gaatcaccac ctccagcaat agcgactttt tttccagcat acttcatcaa
      961 atcattcacg aaataatcca atccgtggtt ttcaaatgat tcagcattat ctaaattcaa
     1021 ttttcttggc tggaatgaac catttcctaa agctaaaatg actgctttgg aataatggat
     1081 gcctttattt gtagtaatct cgataacttc gtcttcacga gtaagcgaga gaacttcttc
     1141 ttttaaatgg aatgtatggt taaaagtagt taattgtttt tctaattgat cgattaattc
     1201 acttgctttt attgctggat acccaggaat atcgtaaata tatttttcgg gatacaaagt
     1261 tgctaattga ccaccaagtt gaggtagact gtcaatgatt tttgttttag cttggcgcat
     1321 tcctgcgtaa aaagcggcaa acatccctac aggacctgca ccgatgattg taatgtcata
     1381 gatttccata aaaattcctc ccttttcaca ctatagcaga attta
//
LOCUS       DACENJ010000321         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.341, whole genome shotgun sequence.
ACCESSION   DACENJ010000321 DACENJ010000000
VERSION     DACENJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.341"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(150..1337)
                     /locus_tag="IW748_002651"
     CDS             complement(150..1337)
                     /locus_tag="IW748_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1105539.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACENJ010000322         1416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.342, whole genome shotgun sequence.
ACCESSION   DACENJ010000322 DACENJ010000000
VERSION     DACENJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.342"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..809
                     /locus_tag="IW748_002652"
     CDS             <1..809
                     /locus_tag="IW748_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1105540.1"
                     /translation="YTPQTMSNITKSFTEEVTAFNGRELHDRYAAIYMDATYIPLKRK
                     TVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERGLKNVLLFITDGL
                     KGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKEVALEA
                     RGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIESFNKQIKKYSHR
                     KEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            1304..>1416
                     /locus_tag="IW748_002653"
     CDS             1304..>1416
                     /locus_tag="IW748_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1105541.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGI"
BASE COUNT          473 a          251 c          288 g          404 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaatataa caaaatcatt tacagaagag gtaacggcgt
       61 ttaatgggcg ggagcttcat gaccgttatg ctgctattta tatggacgca acgtatattc
      121 cgttaaagcg gaaaaccgtc gccaaggaag ctattcatat cgcagttggc attcgcccgg
      181 acggatcaaa ggaagtattg agctatgcga ttgcaccgac tgaatccatc acgatttggg
      241 aggaaatttt attggacctt caagagcgcg gtttgaaaaa tgtcctcctg ttcatcacgg
      301 atggcttaaa ggggatggta ggagcgatta gtcggttcta tcccaaagct cgttttcaac
      361 attgttgtgt acacgtttcc cgtaatatca gtcataaagt gcgtgtcgat gatcgtaagg
      421 aagtctgtga tgattttaaa atggtgtatc aagcctcatc taaagaggtg gcgttggaag
      481 cacgtggtac ttttgcggaa aaatggaaaa ccagctatcc aaaagtggtt gaatcgattc
      541 tttcgaacga tcacttgctc actttctatg atttcccctt ggccatacgc aagagtattt
      601 attctacgaa cttgattgaa tcctttaata aacaaatcaa gaaatacagc caccgcaagg
      661 aacagttcca aaatgaagag tcgatggaac gtttcttagt ctcgtctttt gatacttaca
      721 accaaaaatt cctaggtcgc agtcataaag gtttccaaca ggccgaaggc gagctcgaac
      781 aaatgctgag tcaaccgatg gagaattaga aacaatctac agtagggaag aaccattgac
      841 acaaaattat tgacgctccc aactaaaagt gtcaaaataa tgatgaaatc atttagttct
      901 tataaattag aaaagagaaa ataaatattc catatctact ttattggatc cgtaacatga
      961 ctaaattctg aatacttttt aaagcttatc atttggtaaa actgaatata agttaattat
     1021 atagactgaa aattttgaat tttcagaata gaataactta aaagtactga caaaattttt
     1081 taaaggggca atattaatgt tctgagataa tcaaatccta ccgaaaatat caaaaatcca
     1141 atgtgacaca atcagattgt gtctcattcg agggaatcac acaatcccac gtggctctag
     1201 gcagaattga atttttgctt ggggcttttt ccttatattg attttgaatg agacttatta
     1261 tatgttataa tgcaattaag tatcattgga gagggcgaag aaaatgaaga cggtagaacc
     1321 tattcgagac aaaaaaaaga tcgatgcgat gaaggcaatc ttggcttcgg gaaaatatgg
     1381 ccaacgaaat ttggttcttt tttcgattgg gatcaa
//
LOCUS       DACENJ010000323         1412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.343, whole genome shotgun sequence.
ACCESSION   DACENJ010000323 DACENJ010000000
VERSION     DACENJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.343"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            51..644
                     /locus_tag="IW748_002654"
     CDS             51..644
                     /locus_tag="IW748_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1105542.1"
                     /translation="MIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            710..>1412
                     /locus_tag="IW748_002655"
     CDS             710..>1412
                     /locus_tag="IW748_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1105543.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPP"
BASE COUNT          585 a          181 c          254 g          392 t
ORIGIN      
        1 tagttaaagt taacgatgaa aaagaacagc aaaaagcaat aaaagaacaa atgattgaag
       61 aagaaaaggt aagacgtgaa atagaacgtg aaaagaaacg cattgataaa gaagaacgac
      121 agtttaactc agagataact aagttaatgg catacatgca aaaagctagt gctgatgttg
      181 aaaaagaact ctatgctgat aaaattaaag aactggaaga gaaattaaga gaactagaaa
      241 ctgtcaaaga agatgtattg caaagagaac ttaatactag agctggctat gtttatgtca
      301 tcagtaatat cggatctttc ggtgaagatg tctataaaat aggaatgact agacggttag
      361 aacctatgga tagaattaag gaacttagta gcgcttctgt accttttgaa tttgatgttc
      421 atgcaatgat attttcagaa gatgcaccaa cattagaaaa tcaactacat tcgcatttta
      481 gaaaaaacga agtcaataaa gttaatcaac gaaaagaatt ctttaaagta tctcttgatg
      541 aaattgaaaa agttgtttta gaaaattata acggaactgt atcttttact aaactagctc
      601 aagctgaaca atacagaaga tcattagaat taacagatgt gtagtaaaaa gattagcctt
      661 cgggcttttc ttttacaacg ataagaacat acattcgaaa ggagataaca tgaaacgagt
      721 agcattatat atgagagtat caactgaaca acaagccaaa catggagata gtcttagaga
      781 acaaaaagaa accctctatg aatatatcga acaacacaag gatttgaaag tagttaatga
      841 atatgtagac ggtggtattt ctggtcaaaa aataaatcgt gatgaatttc aaaaactatt
      901 acaagatgta aaagaaaata aaatagatct aattttattt acaaaattag atagatggtt
      961 tagaaatcta cgacattatt taaacactca agaaatttta gaaaaacaca atgtttcttg
     1021 gaatgctgtt tctcaacaat attatgatac aactacagcg tatggcagaa ctttcattgc
     1081 acaagtaatg agttttgctg aattggaagc ccaaattgat tcagaacgca tcaaagctgt
     1141 tatggcaaat aaaattgcac aaggtgaagt agtaagcggg aaaactccgt taggctactc
     1201 tatagaaaat aagaaattag taataaatga tgatgctcct atcgtgatag atatattcaa
     1261 ctactttctt tctagtggta gtttaagaaa aactgtttat tatttaggct ctcagtatgg
     1321 aatcgttaga gattatcaaa gtgttaaaaa tatgttgacg aacaaaaaat acattggtga
     1381 attaagaaat aataaaaatt actgtcctcc ta
//
LOCUS       DACENJ010000324         1401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.344, whole genome shotgun sequence.
ACCESSION   DACENJ010000324 DACENJ010000000
VERSION     DACENJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.344"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..129
                     /locus_tag="IW748_002656"
     CDS             <1..129
                     /locus_tag="IW748_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1105544.1"
                     /translation="DLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            205..423
                     /locus_tag="IW748_002657"
     CDS             205..423
                     /locus_tag="IW748_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105545.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            410..>1401
                     /locus_tag="IW748_002658"
     CDS             410..>1401
                     /locus_tag="IW748_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105546.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLG"
BASE COUNT          500 a          193 c          269 g          439 t
ORIGIN      
        1 gacttgatac gaagagcaaa aacagatcat cgtatcaaac aaaaagacga ttatatcttt
       61 gcttctctaa aaaataaagc gttagaacta attgaattgc aaatcagcga aggaatagat
      121 aagcaataag aatagaattg agtcaaatga aatggagggg tatctccact tcatttgaga
      181 actttgaata taggaggggt tattttgatt aaaaatcgtt taatagatca agtttggtgt
      241 ctagatcaaa atacagatag tctcgaatta atcgagacta tttgcttcaa cactatagtt
      301 ctcgatttaa gagctgaaaa tgataattgt gtaactcatg tcataattaa tcaaaaaatg
      361 gctcagaagc tctctgagag cctcagaaaa tgggctaagg gaggtaacga tgaaaataat
      421 tgaaaaaaag attgccaata aaaaaataaa ttggttggtt gttaattttc ttgttctgat
      481 agctatgatt atttttacaa gtaatattgc tcatgcgagt attttcaatt ggtttgatga
      541 cacaaaaagt caaacagagt ttttgaataa ttctctttat agaccttacc taaaaaagta
      601 caatggagat ttccttagtc agttcgggat ttggctcgga tgggctgtgg taaaaggcat
      661 gtttactgtg acagattcga ttcaaaatat gattccagat gtgctggatt tgttcaattt
      721 cattgaaagt acagggctaa ataatgttta tcaatcagcc gtaaatacaa ttgtcattgg
      781 attaatgatc ctttcattga tgtttgttgg ttacaaaatg attactggaa aaggaacagt
      841 ggatttaaaa tcggttggaa tgaacgtagt gatgtctata gcattaatac ttttaatgcc
      901 aaccatgatt tctagtggta ttcaatttgc taaaacattt tataaggact ctactacgat
      961 tacaaattca gatgacggag tcgcttggtc attgataaaa caaggagtaa cagatttagc
     1021 atatattaat aaaacagatc agtactcaag cattgataaa acggaggatc gaaataattt
     1081 aacacgaaaa aattttcaac agacggatct cacacaagtg ttaacggata aagtgattga
     1141 taaattggaa aaagaaaatc caattgcgga caatcttcgt tatgaattag ttgaaaattc
     1201 taataatgaa tttgttgcta caaagttttc tgacaacttc ttatcaacat tttctgatag
     1261 cttgaaatca ggctattacc gataccaagc aaatctatgg gggatttcaa ttgggttgac
     1321 agcgttagca atcgcctatg tgttcagcgc gtttgtgatc attacagcaa tactagaact
     1381 tgcatttaaa cgtgtgttag g
//
LOCUS       DACENJ010000325         1374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.346, whole genome shotgun sequence.
ACCESSION   DACENJ010000325 DACENJ010000000
VERSION     DACENJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.346"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            33..1372
                     /locus_tag="IW748_002659"
     CDS             join(33..474,474..1372)
                     /locus_tag="IW748_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1105547.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DACENJ010000326         1357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.347, whole genome shotgun sequence.
ACCESSION   DACENJ010000326 DACENJ010000000
VERSION     DACENJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.347"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(54..>1357)
                     /gene="brnQ"
                     /locus_tag="IW748_002660"
     CDS             complement(54..>1357)
                     /gene="brnQ"
                     /locus_tag="IW748_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1105548.1"
                     /translation="FGLFFGAGNLIFPVHLGQEAGAATFWANLGFLVTGIGLPFLGVI
                     AIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPRLATTSFEIGLAPFIDPS
                     RQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLVLLGALLLLAFLNPLGSV
                     NQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTIQNMGVTKPAEIAKDTIK
                     SGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIADHYLGTYGSILLAFIVI
                     LACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLAANVGLTNIIQFSLPVLM
                     FIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDGLNACPETIKQTPIVQNL
                     LYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD"
BASE COUNT          485 a          285 c          243 g          344 t
ORIGIN      
        1 caaaattact ttttattgct caaggctgaa cgtctctttc acaacctctt ttatcaatct
       61 gctacttctt ctttcttagc gaaactccaa atcagtccga taacaaaacc gataactgct
      121 ggtacgatcc accccatccc caatttgaaa aacggcaagt agctttccgc aacatataaa
      181 agattttgaa caataggtgt ttgtttgata gtttctgggc aagcgttcaa cccatcaaaa
      241 atcgaagcaa tcaatgtaaa ataagtagtc attcgataaa cagccgtacg ctgcttaaac
      301 aaaggaccaa ctaacactag taaaatcagt gtcattgcta atggatagat gaacatcaaa
      361 acaggcaacg aaaattgaat gatattcgtc aagcctacgt ttgccgctaa acaaggaagg
      421 atactagcaa gtgcaacaaa gaatgcgtaa tttctttgcg gaaacaaatc tgtgaatgtt
      481 tctgcaaaag ctgtaatcaa accaataccg gtcttcaagc aagctaatat aacgataaat
      541 gctaataaga tactgccata agtacctaaa taatgatcag caatctgagc cagcgtgatc
      601 ccgccatttt cacttagagc aaagctacct aagctcatag cacctaaata agcgagcaaa
      661 gtataaatga ttcccatcaa tataatgctg atcgcaccag atttgatcgt gtctttagcg
      721 atttcagcag gttttgttac acccatgttt tggattgttg tgacaatgat gatcccaaat
      781 gctaaagcag caagcgcatc taatgtgtta tatccttgag taaaccctgt gaaaaaagca
      841 tgctcttgat aatttggctg aataggcgct tgatttacag aacctagtgg attcaaaaaa
      901 gcaaggagaa gcaaagcacc taataaaacc aaaaatgcag gattcaggaa ctttccaaca
      961 taatctaata tctttgtcgg ttttcttgaa aaccaccaag ccagcacaaa aaatacaata
     1021 ctgaaaattg ctagatatag cgtctgtctg ctaggatcga taaatggtgc taaaccaatc
     1081 tcgaaagatg tggttgcaag gcgaggtaaa gcaaaaaacg gtccgataac taagtaaagc
     1141 aagatggtaa aaaccatcgc atatgtttga tttatccgtt ttgctaattc atatacacct
     1201 gagcttttcg atacaccaat ggcaatcacc cctaagaagg gaagaccgat accggtaacc
     1261 aagaaaccta gattcgccca aaaggtagca gcgcctgctt cttgacccaa atgaacggga
     1321 aagataagat ttccagcacc aaaaaataga ccgaaaa
//
LOCUS       DACENJ010000327         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.348, whole genome shotgun sequence.
ACCESSION   DACENJ010000327 DACENJ010000000
VERSION     DACENJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.348"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1337
                     /locus_tag="IW748_002661"
     rRNA            <1..>1337
                     /locus_tag="IW748_002661"
                     /product="23S ribosomal RNA"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DACENJ010000328         1322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.352, whole genome shotgun sequence.
ACCESSION   DACENJ010000328 DACENJ010000000
VERSION     DACENJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.352"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            114..293
                     /locus_tag="IW748_002662"
     CDS             114..293
                     /locus_tag="IW748_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105549.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            424..693
                     /locus_tag="IW748_002663"
     CDS             424..693
                     /locus_tag="IW748_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1105550.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            686..943
                     /locus_tag="IW748_002664"
     CDS             686..943
                     /locus_tag="IW748_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1105551.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          494 a          157 c          251 g          420 t
ORIGIN      
        1 tggtattttg tttgatcttt tggcgctttg tattagccct tgtgagtcta ctattcaaga
       61 tgattgcagc catatttttc atagggttat ttcgatgaaa ggagagagta ctaatgccaa
      121 ttgcaatagg aaataaacgg ttacccgtta cgttggatga aaagagacaa aaagaattgc
      181 agcaactaaa gcagaagtac ggcaaaagtg aatccaggat tatgtgtatt gcgttagatt
      241 tattgattgc ccaagaaaaa gcaggatttg aggtaccagc actcaaaaag tgacgtcact
      301 ttttatccta aaaactaaaa gtgatagcac ttttaattat aagaagttag aatattaatc
      361 atttgcttaa ttgtacaata taatgtacaa ttgttttata gaaataaata aggggtgaaa
      421 ggaatggaag cagtagctta ttcaaatttc cgccaaaatt tacgtagtta tatgaaacaa
      481 gttaatgagg atgctgaaac acttattgta acaagtaaag atgtagaaga tacagttgtt
      541 gtattatcaa aaagagatta tgattctatg caagaaacgt tgagaacact ttctaataat
      601 tacgtcatgg aaaaaattcg tcgaggagat gaacaattct ccaaaggtgc atttaaaaca
      661 catgacttaa tcgaggttga atctgatgat taaggcttgg tctgatgatg cttgggatga
      721 ttatctttat tggcatgagc aaggaaacaa aagcaatata aaaaagatta acaagttaat
      781 aaaagatatc gatcgttccc cctttgctgg attaggaaaa cctgagccat taaagcatga
      841 tttatctgga aaatggtcca gaagaattac agatgaacat agactgatat atagagttga
      901 aaatgaaacg atatttattt attctgcaaa agatcactat taaccaatcg gaagtaagga
      961 aagggtcaga aacttaaaag tttttgatcc ttattttatt taccctagtc atttaaaaag
     1021 ctaatatagc ttagtgttga ttgttattaa tgaatgtgtt tgttacgcgt attacggata
     1081 taaggttagt aaaatcattt ctaaagttga ggaaaagtaa atataaatgg cttaaatttc
     1141 aacaatttga agttgaatag atatgttata atactattgt agtgtgggat gttagttact
     1201 aaaggatgac gcttatatat atgactgaat agaataagca ataggtttaa taatctattt
     1261 taaatttttt gtactagttt tagttaatta gcaaaaacaa caaaaataaa cttctcatag
     1321 aa
//
LOCUS       DACENJ010000329         1322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.353, whole genome shotgun sequence.
ACCESSION   DACENJ010000329 DACENJ010000000
VERSION     DACENJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.353"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>1322)
                     /locus_tag="IW748_002665"
     CDS             complement(<1..>1322)
                     /locus_tag="IW748_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1105552.1"
                     /translation="KQMDIVDFARNNGLAVVNKGRDYRLEDHDSFVFDRRKQRFYWNS
                     QGIGGDIIELTQLFFIDDSIQNPKQRFKAALDYIIKDENKVERVENLHFETQEYKHHP
                     IDYRPLTEKGRSYLKEQRKFPEWLIDYGEQQGLILEMKPRNERKNYIVQDDRLDYAIV
                     FPWKDRHTDQILGATYQGTIVDYERFGKRGVDKRIDINPTPNHGFNVLLGSPKNLKFF
                     ESPIDLLSYAALNRETIKDTWLVSMDGLKDAVVGHYVREAVTELAPKEEFPESIEICV
                     DNDRAGHVFWDKVHRLGVSNPFTGKAVYFEPNLPNDWQVPREYQTIYEEVGKEMRVAP
                     EAIMAIHKIENNLTDTNQIVSFGEIQGVFAKKLAPKEKAQSIVVKEKCIEAAQQLKSC
                     EKSDGTYDFDRFYRGKGKINEQIQFSLKAKHYFEGYKNHEHEFVSEVK"
BASE COUNT          355 a          294 c          210 g          463 t
ORIGIN      
        1 tttttcactt cagaaacaaa ttcgtgttca tgatttttat aaccttcaaa atagtgtttg
       61 gccttcaatg aaaattgaat ttgttcatta attttccctt ttccacgata aaaacgatca
      121 aaatcgtatg ttccatcaga tttttcacac gattttagct gctgagctgc ttctatacat
      181 ttttccttta caacaataga ttgggctttc tcttttggcg caagtttttt ggcaaatact
      241 ccctggattt caccgaacga tacgatttgg ttagtatccg ttaggttgtt ttcgatttta
      301 tgaatagcca tgattgcttc aggcgcaact ctcatttctt tccctacttc ttcatagatt
      361 gtttgatatt ctctcggtac ttgccaatcg ttgggaagat tcggttcaaa atacaccgct
      421 tttcctgtaa atggattcga cacaccaagt cggtgtacct tatcccagaa tacgtgtcca
      481 gctcgatcat tatccacaca aatctcaatc gattctggaa attcttcttt cggcgccaat
      541 tctgtcaccg cttctcgaac atagtgcccc actactgcat ccttcagtcc atccatagaa
      601 acaagccacg tgtctttgat cgtctcgcga tttaaagcag cgtaactaag taaatcaatg
      661 ggactctcaa aaaatttcag attcttagga gatccaagaa gaacattaaa cccgtgatta
      721 ggcgtaggat ttatatctat tcgtttgtca acaccccgtt ttccaaaacg ttcatagtct
      781 acgatagtcc cttgataagt tgcccctaat atttggtctg tatgacggtc tttccaggga
      841 aaaacgatcg catagtctaa gcgatcatct tgcacaatat aattttttcg ttcattgcgt
      901 ggcttcattt ccaggatcaa cccttgttgt tctccgtaat caatcaacca ttcagggaat
      961 tttcgttgtt cttttaagta acttcgtcct ttttctgtca atggtcgata atcaattggg
     1021 tggtgcttat attcttgtgt ttcaaagtga agattttcca cccgttccac cttattttcg
     1081 tcctttataa tataatctag cgctgcttta aaacgttgct ttgggttttg aatagaatcg
     1141 tcaataaaaa acaattgagt caattcaata atatccccac caataccttg actgttccaa
     1201 taaaatcgct gttttcttcg atcgaaaaca aaagaatcat ggtcttctaa acgatagtct
     1261 cgtcctttat tgactaccgc taaaccattg tttcgagcaa aatccacaat atccatttgc
     1321 tt
//
LOCUS       DACENJ010000330         1310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.355, whole genome shotgun sequence.
ACCESSION   DACENJ010000330 DACENJ010000000
VERSION     DACENJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.355"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            129..>1310
                     /locus_tag="IW748_002666"
     CDS             129..>1310
                     /locus_tag="IW748_002666"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1105553.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
BASE COUNT          410 a          262 c          236 g          402 t
ORIGIN      
        1 gctctataat ttctaggact gatggaaatt ttctatctgt cctagttttt ttgtaaaaaa
       61 acaaaaagac atacaaaatt tccggtagaa tagagtctcc tacaacatca ttctggggga
      121 attcttgtat gtccatgaat cattttatca aacaactctt tactattaaa gactcaaata
      181 tccaaattca atcctttgaa gaaacttttt ataaaagcag aaaaagtatc gttattgagg
      241 gaactctgat ccgtacctac agacgttgtc cgtgttgtaa agattctaca aagcaaatcg
      301 ttaaaaatgg caagaagatc tctatgattc tcctgaatcg tagcggtaat aaacggacct
      361 atcttcggtt gaagaaacag cgatacctct gccgagcatg taaaaaatat tttaccgctc
      421 ggacgtatct cgttacccca ttctgcttta tttctaagca gattcattac aagatcttag
      481 aagaattgac agagcgtcaa tctataaaag caattggtaa acattgcgat gtttctgtga
      541 caactgttca gcggacattg tccgccttgt catcagatat ccgtcagaat cttgagtatt
      601 tacctcggtg tctattattc gatgagtttc gttctctttc tacatggcac ggtaaatttt
      661 ctttttcttg tatggacggt gagaccggga aactgtttga gatcttacct agccgccgta
      721 aaaaagatct cgttgctttt ttcatgcgtt tttctctcaa agcgaggttg ggtgtacatt
      781 acatcgtaac ggatatgaac gctccttatt tctcactagt taaagagtgt tttccaaatg
      841 cacaagtgat cgtcgatcgg tttcacattg ttcaacatct gaaccgcttc ttcgatgcgg
      901 ttcgaaaacg tgtgatgaaa acacttgatc aaaaagaccc aatacaagct aaacagtatc
      961 gccaattgaa atccttattc aaattacttt taaaatcaga ggatcgttta gattacaata
     1021 cactaacgaa aagacgcaat ttcaattgga gactattgac tgaaacagaa gtagttgacc
     1081 gccttctaaa gatttctcct gaacttaaaa cagcttatcg ttactaccaa gacctgctta
     1141 ctgcgtatca ggaaaaagat ccagatactt ttttccagct gcttcgagca atgcctggag
     1201 aagttccatt ggaactgcac catatcaaaa aagccttttt caaatacgag aaagggattc
     1261 ggctggcatt actgaagaag tattcgaatg cacgactgga aaatctccat
//
LOCUS       DACENJ010000331         1268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.357, whole genome shotgun sequence.
ACCESSION   DACENJ010000331 DACENJ010000000
VERSION     DACENJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.357"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            37..588
                     /locus_tag="IW748_002667"
     CDS             37..588
                     /locus_tag="IW748_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ1105554.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          482 a          171 c          231 g          384 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggc
//
LOCUS       DACENJ010000332         1265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.358, whole genome shotgun sequence.
ACCESSION   DACENJ010000332 DACENJ010000000
VERSION     DACENJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.358"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..986)
                     /locus_tag="IW748_002668"
     CDS             complement(<1..986)
                     /locus_tag="IW748_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1105555.1"
                     /translation="MKEERKLPEWLIDYAEKEGLIAELKPKHERQNFLVGDDRLDHAV
                     AFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKF
                     FESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRRKQTFPQSIEIC
                     VDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVE
                     PEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKV
                     CERADGTYNFDRFYSRKANIKDVNAGILISYKAEQYYKGYKKHEHEFVPEVK"
BASE COUNT          327 a          264 c          200 g          474 t
ORIGIN      
        1 tttttcactt caggtacaaa ttcatgttca tgctttttat agcctttgta gtattgttct
       61 gctttatagg aaataaggat tcctgcatta acgtctttta tgttggcttt tctactataa
      121 aaacgatcaa agttgtatgt tccatctgcg cgttcacata ctttcagttc cttcgcgacc
      181 gtggtacatg cttctttcaa atcaattgtt tctactggtt cgcccttcgc caacatcttt
      241 ccaaacgtag attgaacatc atgagcagac accaattgat ttgtcagctg taaattagtt
      301 tcagttttgt gtatggccat aattgcttca ggttcaacac tcatttcttt tgcgactgct
      361 tcataagtag ctttgtattc tttaggtact tgccaatcat ttggaattcc tcgctcacaa
      421 cggattttct tattggtaaa aggatcaacg attcccttca tctgttcttt ttcataaaaa
      481 atatgtcctg cacgatcatt gtccacacat atttcaatcg attgaggaaa tgtttgtttt
      541 cgtcttaatt cagaaatcga ctcttcaacg taatgactga ttacatgatg ttttaacccg
      601 tccatcgaaa ctaaccaggc atcttgtagt ttctcccgat tcaatgcagc gtaacttaac
      661 agatcaatac tactttcaaa gaattttaaa tgcttcggat cgcctatttt taaattaaat
      721 ccgtgatttg gcgtaggatt cttgtcaatg tgtttatacg ttccccgttt accgaaacga
      781 ttaaaatcga cgatcgttcc ttgataactt gcccctactg tctctcctgt ctgtggatct
      841 ttccataaaa aagcaaccgc atgatctaaa cggtcgtctc caactaaaaa attttgccgt
      901 tcatgtttcg gctttagttc agcaatcaac ccttcttttt ctgcataatc aatcagccac
      961 tctggtagtt ttcgttcttc cttcagataa ttccttcctt tttctgtgag tggttgataa
     1021 tcaacggggt gatctttgta cttttctgtc tcaaaatgaa gattttccac tcgttctgtt
     1081 ttgtcttcat tctttagaat aaaatcagtg gctgctttaa attgttgctt agggtcttgg
     1141 atctctttgt ctatgaaaaa aagtttagct aattcaataa tgtcgccact aatattttga
     1201 ctgttccaat aaaaacgttg ttttcttcga tcaaaaacaa atgaatcatg gtcttctaag
     1261 cgata
//
LOCUS       DACENJ010000333         1257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.361, whole genome shotgun sequence.
ACCESSION   DACENJ010000333 DACENJ010000000
VERSION     DACENJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.361"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..760
                     /gene="mreC"
                     /locus_tag="IW748_002669"
     CDS             <1..760
                     /gene="mreC"
                     /locus_tag="IW748_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1105556.1"
                     /translation="NEGKPNFFQSIVNDSVAMVDKTISAPVRWVRNGVDSVHNLFSTY
                     AENERLKEKIDSYDDVSQQNKNYEREIEALKKELELNQTLTSYEKVTANVITRSPDSW
                     QDMLIVDKGSKDGIEANMAVMSQKGLIGRVIEVNTASSKIELLSSSNESSNHFPVRVS
                     SANGEAFGLLKNYDEKLHALVVTQLTGDTDIKEGDVVQTSGLGGNSPANLPIGTVIKT
                     KPDSYGLDREVYVKPYAEMYDVSVVTIVQRLAED"
     gene            764..>1257
                     /gene="mreD"
                     /locus_tag="IW748_002670"
     CDS             764..>1257
                     /gene="mreD"
                     /locus_tag="IW748_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1105557.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKK"
BASE COUNT          375 a          213 c          283 g          386 t
ORIGIN      
        1 gaatgaagga aagcccaatt tcttccaatc catcgttaat gacagtgttg caatggttga
       61 taaaacaatc tctgccccag tacggtgggt acgaaatgga gtagattctg tccataatct
      121 tttttcaact tatgccgaaa atgagcggct aaaagaaaaa atcgatagct atgatgacgt
      181 ttcacagcaa aacaagaatt acgagagaga aatcgaagcg ttgaaaaaag aacttgagtt
      241 gaatcagacg ctgactagtt acgaaaaagt aacggcaaat gtcattactc gttcgcctga
      301 cagttggcag gacatgctga tcgtagacaa gggttctaaa gacggtatcg aagcaaatat
      361 ggcagtcatg tcgcaaaaag gattgattgg ccgagtgatc gaggtcaata cggcttcgtc
      421 taaaatcgaa ttactgtcat cctctaatga aagctccaat cattttccag tacgggtatc
      481 ttcggctaat ggcgaagcgt ttggtttgct taaaaactat gatgaaaagc tccatgcctt
      541 agtggtgacc caattaactg gtgatacgga tatcaaagaa ggggatgttg tccagacatc
      601 cggtcttgga gggaattctc cagctaactt gccgatcggt acggttatta aaacgaaacc
      661 agatagttat gggctggatc gggaagttta tgtgaaacct tatgcagaaa tgtatgacgt
      721 gtcagttgtg acgattgtcc aacggctagc ggaggattag tccatgttga agaaagaaac
      781 aatgaagtac tatctgccaa tcgttttgtt ctttttgatg ttgatagatg gtcatttaac
      841 aagaatgcta ggggagtggt cgaaaggcac ctatatgtca aatgcccact ttctgatatt
      901 ggcattatta tgttgcagta tggcgtttga aaaacgttat ttactgatta ccacgattgt
      961 tctcggggct atctatgacg cttactatat tggcgttatc ggtatctatg cagtagctct
     1021 ccctttaatt gtatggttga tgtatgtaat gaaagacgtt atccatgtca acatctttac
     1081 tgaatttttc agtatgatca tctttgtcac gggttatgaa ttgtttacga tggtggtcca
     1141 gttgattttt aaattagcag tagtaaataa cacgtatttt attacaaggt ttttaggacc
     1201 tacactgctg ttgaacatga ttatatttgt attattcatt tttcccttta agaaatt
//
LOCUS       DACENJ010000334         1244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.362, whole genome shotgun sequence.
ACCESSION   DACENJ010000334 DACENJ010000000
VERSION     DACENJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.362"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1106
                     /locus_tag="IW748_002671"
     CDS             <1..1106
                     /locus_tag="IW748_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1105558.1"
                     /translation="SLSAFSTGTILGWQFYNNEHSQIVAHAQSAQKKLILTTNEQQLV
                     EKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKALTT
                     LLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTDEEV
                     VQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHTKFY
                     DQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQVLSG
                     KLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAIIME
                     SNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          442 a          237 c          205 g          360 t
ORIGIN      
        1 ttagccttag tgcgttctct acaggaacta ttcttggatg gcaattttac aataatgaac
       61 actctcagat agttgcccat gctcaatcag ctcaaaaaaa gttgatactg actaccaatg
      121 aacaacagct cgttgaaaaa atcgatcaac tattaagaaa aaatgattat attggctcaa
      181 tttacgtaaa gaaaaataat cgcatcatca tggaaaaagg ctatggctat gcgaataaac
      241 tcacgcaaca accaaatgat ccttctcttt attatcagat tggttcggtt caaaaagcat
      301 tgactactct tttgatttta aaacaagtcg agcgtgggca cctttcttta gatactaagc
      361 tggctcaatt ctatccacaa attgctggca gccagcaaat tactatccgt gacttgctgt
      421 atatgcgctc aggcttgcac cgaactgctt ctccaaccat accgatgact gatgaagaag
      481 tcgttcaatt tgcgattcag cacttaaaat ttacagatta tcagacctct cattatgatt
      541 cattgaactt taccatattg actggaattt tgatcaaatt aacaaatcaa ccgtatgaaa
      601 aattattgac cgatgatatc atcgaacctt taggattaca gcatacaaaa ttttatgacc
      661 aagtcaaaaa ttcatccaat catgcactat cctatcaaat gtctaaagag gatgattact
      721 ttaaaccact gacagaatct gaaacagaga tccataacga attagggact ggtaatatca
      781 gtatgtccgt ctatgattta aatacgctgt ttacacaagt tttatctgga aaactagttt
      841 ccaaagactt actcttctca ttatggcgtg aaaatgcgaa tggacatcca tatagtggtg
      901 gggtatatag cggagataat tatatcctgt cgcaaggaaa tatcgactct tttgatacat
      961 ctgctatatt tagcaaagat tcacaggatg ctatcatcat ggaaagcaat ataaaagctg
     1021 acaaaactat caaactgcct actgctgatc taagaaacca aatctatgac ctgatggaag
     1081 gaactactaa gttggcaaat agctgatatg atgaaaagat agcaaatact acttctttga
     1141 atagcatttg cttaaaaaaa gtactcgact aaacgtagga aagagtgtgg gacaaatcta
     1201 aaaatcaatt tttgctacag actttaattc caaataaaca gaac
//
LOCUS       DACENJ010000335         1229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.364, whole genome shotgun sequence.
ACCESSION   DACENJ010000335 DACENJ010000000
VERSION     DACENJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.364"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1039
                     /gene="murA"
                     /locus_tag="IW748_002672"
     CDS             <1..1039
                     /gene="murA"
                     /locus_tag="IW748_002672"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1105559.1"
                     /translation="EYVSQMRASIVVMGPLLARNGHAKVAMPGGCAIGKRPIDLHLKG
                     FQALGAKIIQKNGYIEAIADELIGNTIYLDFPSVGATQNIMMAAVKAKGTTIIENVAR
                     EPEIVDLANILNKMGAQVYGAGTETMRIEGVDHLHAVNHSIVQDRIEAGTFMVAAAMT
                     QGNVLIADAISEHNRPLISKLIEMGAEIIEEEGGVRVIGPKHILPTDVKTMPHPGFPT
                     DMQAQMTAIQLVAEGTSVVTETVFENRFQHLEEMRRMNAHVKIDGNVAIMDGNHELQG
                     AEVYATDLRAAAALVLAGLKANGITRVRNLNYLDRGYYNFHIKLQQLGADVERVDMDQ
                     TSAEKTAQTIA"
     gene            1052..1228
                     /locus_tag="IW748_002673"
     CDS             1052..1228
                     /locus_tag="IW748_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1105560.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
BASE COUNT          393 a          221 c          275 g          340 t
ORIGIN      
        1 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
       61 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
      121 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
      181 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
      241 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
      301 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
      361 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
      421 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
      481 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
      541 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
      601 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
      661 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
      721 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
      781 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
      841 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
      901 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
      961 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
     1021 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
     1081 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
     1141 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
     1201 gattcatatt cgagactttt ttcattgat
//
LOCUS       DACENJ010000336         1200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.366, whole genome shotgun sequence.
ACCESSION   DACENJ010000336 DACENJ010000000
VERSION     DACENJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.366"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(172..786)
                     /locus_tag="IW748_002674"
     CDS             complement(172..786)
                     /locus_tag="IW748_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1105561.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          388 a          239 c          210 g          363 t
ORIGIN      
        1 acaaacttta aacaaaacaa gcttaaaatc aaagatgatc catcttaaaa taactaaaac
       61 aaaaaaagta gtcgttgtta atcctcatta ataatcgttt tctttttttc aaacgctcca
      121 tttttatgtg gaacattatt tttttaagga actttgtcaa catgtggata actattttcc
      181 acttgtgtcc tggactagct gttttgctgt aaaatacaaa acttgctgca gcaaaaagat
      241 cgttgcaaga gaggctgttc cgcagatcgc tggatctaga tttggcaaag tcaagactgc
      301 atcatgtaaa ttggcgtggt tgtccaaaac caaatcagca aattcaaata atcgcaaacc
      361 actgctatgt tgcgatttga tacttcgtga caaatcaaat ccagtaacaa tgattaatgg
      421 atgaccattt tctttgatcc actgagctag ttcgatgatt gccgggtttc ttccttcatt
      481 cgaaagaaga agaaaaatgt cttctgattt gacagaaagc tgatcaataa ttgcctgtcc
      541 tgcaccttct agcgtttcat agacacctcc ggctggattt tcaatcaata tacttggtac
      601 aagatccggg gtttgatccc aaaattcaaa tgctacacca cttagtgtgc ggctagcata
      661 tatctgcaat ctaccacctt gattaatttg tttcgcgatc caagtgccag cttgaagcat
      721 cagcggctct tctatcctta atttctcttc caattggtga taatagtcaa aataacataa
      781 actcatttat ctctcccgcc tagctatact tgacatgaat agaaacagct gcctaatcta
      841 aggaagtaca taaaaaaaca gtagttagac agtaacacat gtggtctttg ctgtttcaaa
      901 cacaaaaaag gcattgaacg aatacgccca ataccttgac atagcaaata aaacaacgtg
      961 cagaccatgg aaaatatcta cataggggta gaattttcac cctagattag gtacaataaa
     1021 ttaggtcgat gcactgcatt gactatggca aaatgatcgg tcagggtgta tgtgtgtgac
     1081 tggtcattcc ctgattctta gcgccaacta agggtcaggg agcctttttt tattcagctg
     1141 tttctatctg aatttattgt aagtaatctc ttatccaagt gcaagcaaga aaatacaaaa
//
LOCUS       DACENJ010000337         1196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.367, whole genome shotgun sequence.
ACCESSION   DACENJ010000337 DACENJ010000000
VERSION     DACENJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.367"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(44..1027)
                     /locus_tag="IW748_002675"
     CDS             complement(44..1027)
                     /locus_tag="IW748_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1105562.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          330 a          264 c          227 g          375 t
ORIGIN      
        1 cagctttttc acgacctcta attcacggtg ttcaagcaag aaatcattgt tcactgaaaa
       61 gcgttggatg ttctttttca aaggtctcta agatctctcg tacttcatca gtagatttcg
      121 tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa tcctttgata tagattttaa
      181 agaaacgatg tagtcctgtg atggaacgcg ggatgatttc tgaataatga tcttgcagat
      241 ctagttgcag acgaagtagt ccgatcagtt ctttttctgt atgttccctc gggtcttttt
      301 caaatgcata aggattcttg aaaatccctc ggccaatcat gatgccatca acgccatatt
      361 tctcggccaa ttcttctcct gtctggcgat cggggatatc tccattgatc gtgattagtg
      421 tttgtggagc gatttggtca cgtaaagcaa tgattttagg aatgacttcc caatgtgcat
      481 ctactttact catctcttta cgcgtacgca aatggacaga aagattggct atatcttgat
      541 gaagtaggtg actgatccat tcttccatct cagacatttc cgtatagccg atacgcgtct
      601 tgacgctgac aggcaaaccg ccagctttag ctgcttcaat caattgtgcc gctacttctg
      661 gacgaaggat caagccactg ccttttccac gttctgctac attagggact gggcagccca
      721 tgttgatatc gattccttgg aagcccattt ctgccatagc aatgctcatt tctctaaaaa
      781 attctggctt gtctccccaa atatgggcaa ccatcggctg ttcatcttct gtaaatgtta
      841 atcttcctcg tacactgtct tttccatctg gatgacagaa actgtctgag tttgtaaact
      901 cagtaaagaa gacatccggt gctgctgcat gctttacgac atggcgaaat acaacatctg
      961 ttacatcttc cattggtgct aaaacaaaaa aaggctttgg cagctctttc caaaaattag
     1021 tagtcattta tttgttactc tccattctat caagcagtga agtcgcaatc tttctcaaaa
     1081 acattgtcat tatactagga aacaaaaaaa gaagcaaagt gaacagctta aaagatgttt
     1141 ctgattgagc aaaaaaaaga ctgtgacaaa aatcttatca cagtccattt tccgga
//
LOCUS       DACENJ010000338         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.368, whole genome shotgun sequence.
ACCESSION   DACENJ010000338 DACENJ010000000
VERSION     DACENJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.368"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            224..1135
                     /locus_tag="IW748_002676"
     CDS             224..1135
                     /locus_tag="IW748_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1105563.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
BASE COUNT          436 a          198 c          237 g          324 t
ORIGIN      
        1 acgaagaaat taattgtttg tagaatgaag gaaactattt attgggtaga tgaatcacca
       61 cgaatagtca aaaggagatt tcaggatatg ccaaaaatga aaaaaatcat tttagcagta
      121 atgttatttt ctacggttat atcttttaca gccccttttt tgcgtactta agctgatttt
      181 aagttttgac ccttagtctg gatctacgag gtgagagtca agaatgaaaa aaataattgg
      241 agcttttgta tttgcaatca ttatcattat tatctgtttt caaatagatg tttttggtca
      301 aaaaaagata gttggacaaa cggtcagcag tacgaacagt acggttacaa agacaaagga
      361 atcatttgat gatacagaac aagagacatt aatcaaaatc ggaaaaaatg accgtacact
      421 atatacagat gactcgctaa ctaccccact tgcaactata aacggcggag agctggcgga
      481 gtttctttct gaaacaaaaa atgcttatca gatcaagaca aatgatggct atactggata
      541 tttagccctc gaagatggaa ccaaagtcac aaagaatata caaacaaaac cggaagagct
      601 atctgatgca gtgattgtat tagatcctgg gcatggcgga aatgatactg gggcattaag
      661 caatgatgaa caaacagaag aaaaagatat aacattaagt acagccataa aagtaaaaaa
      721 ggcgctagag gatgctggag caacggttta tttgacccat actacagatg aattagtaca
      781 gcttggagat atatgcgact acagtgaaga aaaaaaggca gatgtcttta tcagtttaca
      841 cgcagactct acggaatatg cgaatgaagc aactggaatc acgacatatt attactatgg
      901 acaagaggaa acattagcac agacaattgc tgactccttc actgatctgc ctctggattc
      961 aagaggaatc tccactggga actatcaagt tcttcgagaa aatcttcaac cttctatttt
     1021 aatcgagatg ggttatatga ataatgatag tgatttaaag gaattagtaa cagacagtta
     1081 tcagcagcag attgctgcaa gtttgacaca agcattgacc acttattttc agtaataaat
     1141 aagcccagcc aaacaaaaat agctcctcat atttttcgtt tggccgggct tatta
//
LOCUS       DACENJ010000339         1184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.370, whole genome shotgun sequence.
ACCESSION   DACENJ010000339 DACENJ010000000
VERSION     DACENJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.370"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..403
                     /locus_tag="IW748_002677"
     CDS             5..403
                     /locus_tag="IW748_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1105564.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IW748_002678"
     CDS             387..1082
                     /locus_tag="IW748_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1105565.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          453 a          172 c          250 g          309 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctta
//
LOCUS       DACENJ010000340         1165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.371, whole genome shotgun sequence.
ACCESSION   DACENJ010000340 DACENJ010000000
VERSION     DACENJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.371"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1165
                     /locus_tag="IW748_002679"
     CDS             <1..>1165
                     /locus_tag="IW748_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1105566.1"
                     /translation="TLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALRILLD
                     DSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNSESQT
                     ILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDYEFYY
                     TSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVYVDAL
                     RDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIGENIN
                     RQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALK
                     IVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSV"
BASE COUNT          427 a          176 c          186 g          376 t
ORIGIN      
        1 ctactcttca aattcgaaat tttagaaatt atgaatccga aatctttcga ttcgataatg
       61 agacaaatac tattataggt gagaatgatt cagggaaaac caatgctctt actgcattga
      121 gaatcttatt agatgatagt tattactata gtagtaaaac actaaaagaa tcagattttt
      181 ttcatggaat tcaaaatggg tggcaagggc attggattat tatctctgcg acatttgaag
      241 gaatcagcga agaagaattt gacaatgaga tttgtgcttc catttcttta aattctgaaa
      301 gtcaaacaat cttagaagaa ttgatttcaa atgcagataa aggtgtaggt agtatttctc
      361 tatttattag acctaacaaa gctattagaa agcaattatt tgatatttcc actgaattag
      421 actctcatca atttaacgaa tttagaaatt ctattcgtct atccgactat gaattttact
      481 acacatctaa atcaaatcta aatttctgta tcaatgaaaa ttatgataaa ttggtaggaa
      541 gactcaatga caatatagct agtaatcctg aagaagacga tgaagctctg ttgggctcaa
      601 gaattgacat gtcagatatt ttcaagcata tttcggttgt ttatgttgac gctcttcgag
      661 atgttcttag agaaatgaaa aacaatcgaa acccagtcaa aagaatcatg gaaacaattg
      721 aatcaaaaat atcatcagat aatgttgagt cattaaaaac aattatacag caattaaatg
      781 agactattac cagtgttcct gaaattagga aaattggcga aaatattaat cggcaactaa
      841 atagcataat tggttcagtg tattctccta atcttcttct ttcctcaact atgtcagatg
      901 atatgggttc tttagcaaaa tttctttcaa tgaagccaga acaaaatatc gacttagact
      961 tactagggtt aggacatcta aatatgattt atttagcttt gaaaatagtc gaatttgaag
     1021 cgtgtcgttc aagagaacta cttaatatta tgttgattga agaaccagaa gctcatattc
     1081 atcatcatat tcaaaaaact ctgttcgaag gattaaatct tcaaaaaaac tatactcaga
     1141 ttctcatgac cacacattct gtaca
//
LOCUS       DACENJ010000341         1156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.372, whole genome shotgun sequence.
ACCESSION   DACENJ010000341 DACENJ010000000
VERSION     DACENJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.372"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            26..898
                     /locus_tag="IW748_002680"
     CDS             26..898
                     /locus_tag="IW748_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1105567.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          333 a          222 c          279 g          322 t
ORIGIN      
        1 ttctaaaata aagggagaaa tgtctatgct atacggtgca attgaagcag gcggcacaaa
       61 atttgtgtgt gccattggcg atgaagaaat gacgatcaag gaaagagtga gctttccgac
      121 aacgacacct gaggaaacaa tgcctttagt cattgacttc ttcaaacaat accaagcaga
      181 tttagcaggg attgggattg gctcatttgg gcctatcgat atccatcgcg attccgccac
      241 ttatgggtac attacctcga ccccgaaatt ggcatggcaa aattttgatt tcatcggtac
      301 catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg cggctgccta
      361 tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact atacgatcgg
      421 tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt ttagtcatcc
      481 agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg gcagctgtcc
      541 atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa accgcttagg
      601 gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag cctcctatat
      661 tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca tttttggcgg
      721 tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg caaaattggt
      781 caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag cattagaaga
      841 caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac attcataaac
      901 gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa aatttagtca
      961 taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaagatc aagccttgaa
     1021 tgagataaat tcttgctcga aagttatgga aatttcgata gccgtaggcc acttgtattg
     1081 tgcaatagtt ttttacaaaa aaaagggggt gcggatattt ttttggacct ttttaacaaa
     1141 tatatctttg cttctt
//
LOCUS       DACENJ010000342         1152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.373, whole genome shotgun sequence.
ACCESSION   DACENJ010000342 DACENJ010000000
VERSION     DACENJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.373"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1152
                     /locus_tag="IW748_002681"
     CDS             <1..>1152
                     /locus_tag="IW748_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1105568.1"
                     /translation="KGSGEAIGIAGPSVLIAFLIGGLILYIVMKGLGKIVLSGGDTHH
                     GLSGLIRPYLGAHSADFTDWVYWSMWIINIIAEAVAAASFLQLWFPHIPAWVFVFMLA
                     VLTTIINLYSVRLFAETEYWLAFAKISVIILLIIFATYLVGQQMLGTGVFPTLQQLTD
                     HGGFTPHGMKGIISSLLVVIYSYGGSELIAITVSEADDPKRAIPKAIKGVMGRIISFY
                     IIPLFLLLILFPWNTLAGTNVSPFVMVFEKMNIPFAADIVNFVIVLALFSSINSGVYA
                     SSRILFFRLKDRKGSSRKLAVLNKHQVPQRAVLFCTSVLYLGVALSYFVGDKLFGYLA
                     GSLSYTVLLIWIIISAASFVLALRKGTLWDKGISFFALAVLGLIFLGIL"
BASE COUNT          258 a          212 c          251 g          431 t
ORIGIN      
        1 ttaaaggaag tggcgaagcg attgggatcg ccggcccttc tgtattgatt gcttttttga
       61 ttggtgggct gattttatat atcgtgatga aaggtctggg aaaaatcgtt ttaagcggtg
      121 gcgacacgca tcacggatta tctggtttga tacgccctta tctaggtgct cattctgctg
      181 attttactga ttgggtctac tggtctatgt ggattatcaa tatcatcgct gaagctgttg
      241 cagctgcttc ctttttgcag ttgtggttcc cgcacattcc agcctgggta tttgttttta
      301 tgttggctgt tttaactacg atcatcaatc tttattcggt acggttattc gcagaaacag
      361 aatattggct ggcttttgct aaaatcagtg tcattatctt actgatcatt tttgctactt
      421 atcttgtcgg tcagcaaatg ttgggcacag gcgttttccc tacccttcag caattgactg
      481 accatggtgg gtttactcct catggtatga agggaatcat cagttctttg cttgtagtta
      541 tctattctta cggcggttct gaactgattg ctatcacggt aagcgaagca gatgatccta
      601 aacgtgcgat tcctaaagca atcaaaggtg tcatgggaag aatcatttcc ttttacatca
      661 ttcctttgtt tttgcttttg attctttttc cttggaatac tttagcaggc acgaatgtca
      721 gtccttttgt gatggtattt gaaaaaatga atatcccatt tgcagcggat atcgtcaatt
      781 ttgtgattgt ccttgctttg ttctcatcta tcaattcggg tgtgtatgca tcttcaagaa
      841 ttttgttctt ccgtttgaag gatcgaaaag gttcctctag aaaactagct gttttaaaca
      901 agcatcaagt accgcaacgt gctgttttat tttgtaccag tgttctttat cttggtgtag
      961 ctctttctta ttttgtcggt gacaaattat ttggctatct ggcaggttcc ctttcgtata
     1021 cagtattatt gatctggatc attatcagtg cggcatcctt tgtactggca ctaagaaaag
     1081 gaacattatg ggataaagga atcagctttt ttgcattagc tgtcttagga ttgattttcc
     1141 ttggtatcct gt
//
LOCUS       DACENJ010000343         1146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.375, whole genome shotgun sequence.
ACCESSION   DACENJ010000343 DACENJ010000000
VERSION     DACENJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.375"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            405..>1146
                     /gene="msrA"
                     /locus_tag="IW748_002682"
     CDS             405..>1146
                     /gene="msrA"
                     /locus_tag="IW748_002682"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1105569.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRY"
BASE COUNT          416 a          180 c          224 g          326 t
ORIGIN      
        1 aagtaatcac acttcctgta tatgcttatt taggaacgaa tgtagaaata aatcatcaac
       61 ctgtaaagga gcaggtgaat gctaatggat tggtacaaat aagcggagtc aaaggagttg
      121 ctgatattaa agtaagcaca agttatacag taacgactat tatttcttat cttgttacat
      181 taactgcata tttatgttta ttttttaaaa tagttttcca taaaaggtaa attaactttt
      241 catttataaa ataaattgat taaaaaggag aatctgtcga taaggattct ctctttttta
      301 tacgaaaaat ctgtaggggg atgaagtaat cgcttatttc ctcactttat gataaattac
      361 agctaaagtc actagcttgt cttcttcgga aaggaggaga atctatggat gaggaacaaa
      421 tattcattca aacactttat aatctgattt tgaatccgaa tacacgagat tgggaacgaa
      481 aagtattgat tcagacaaag aatgacataa gagaaaatat atccgttaaa gaacagttat
      541 caaaactaga agcaacacta cgtccattag ctatccgaat gaatctgacg cctgatgtaa
      601 tggactttta tttattgatt acggaaggat ttgataagga gcaaaaatat gacttttcca
      661 agcatgccat gcaagatgct gactatcaag aacgtgcagt atttgcaggg ggatgtttct
      721 ggtgtatggt cgagccgttt gaatcgaaaa aagggatcct atctgttttg tcaggataca
      781 cgggcggaca tgttgaaaaa ccaaattatg atcaagtaag tggtggttac acaggtcatg
      841 tggaagctgt ggagatcatt tatgacacaa gagaaataag ttatagcgag ttgctgacaa
      901 tctattggca aattactgat cccacagata catttggaca atttcaagat agagggaagc
      961 aatatcgtcc agtgattttt tatcaaggcg aacgacaaaa agaattagcg gaacaatcaa
     1021 aacagaaact agacagttca ggaactttcc accaaccaat cgtcacaaaa atcgaaccag
     1081 ccggtacgtt ttggccagca gaaaactatc atcagcaatt ttacaaaaaa caaccgaaac
     1141 gttata
//
LOCUS       DACENJ010000344         1137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.378, whole genome shotgun sequence.
ACCESSION   DACENJ010000344 DACENJ010000000
VERSION     DACENJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.378"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1086
                     /locus_tag="IW748_002683"
     CDS             <1..1086
                     /locus_tag="IW748_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1105570.1"
                     /translation="IAAADLQVGEEEKLKEEREKLSNYQKIADGLAAGYGALTNNEQN
                     SVDGVGLAVSELQGIAHLDVEYEAIYENIQSAYYLLQDAIGDMSRQIDLLELDESRLE
                     EVTQRLELIRQLKRKYGESIESILAYYDEITEELASSDFSEGQLDKMKEELEQKELLL
                     QQQAADLHEARKKIAKELEKSILHELKSLYMENTEFEVRFLKEENRQLNHDGFDQIEF
                     YITTNPGEPLKPLVKVASGGELSRMLLALKTIFSSEQGVTSIIFDEVDTGVSGRVAQA
                     IADKISQISKYSQVLCITHLPQVAAVADYQYYIVKAVIDGRTQTSVSELKTKEREEEI
                     ARMLAGSEITKLTLEHAKELLKLAKRT"
BASE COUNT          388 a          190 c          258 g          301 t
ORIGIN      
        1 atagctgctg ctgatttgca agtcggagaa gaagagaaat taaaagaaga aagagaaaaa
       61 cttagcaatt atcaaaaaat tgctgatgga ttagctgcag gttacggagc actgacgaat
      121 aatgaacaaa acagtgtaga tggtgttggc ttggccgttt cagaactgca agggatcgcc
      181 catctagatg tggagtatga agcaatttat gaaaatatcc aaagtgctta ctatttgctg
      241 caagatgcaa tcggcgatat gagtcgtcag attgatttat tggagttaga tgagagtcgt
      301 ttagaagaag ttacgcaacg tttagaatta atccgacagt tgaaacgtaa atacggcgaa
      361 tccattgagt caatattggc ttactatgac gaaatcaccg aagagctagc ttcttctgat
      421 ttttcagaag gtcaattgga caaaatgaaa gaggaactag agcaaaaaga gcttctactt
      481 cagcaacaag ccgcagatct tcatgaagcg cgtaaaaaaa tcgcaaaaga attggaaaaa
      541 tccattctgc atgaattgaa aagtctgtat atggaaaaca ctgagtttga ggtacgtttc
      601 ttaaaagaag aaaatagaca attgaatcat gatggatttg accaaatcga attttacatt
      661 acgaccaatc caggagagcc actgaagcct ttggtgaaag tcgcttctgg tggcgaactg
      721 tctcggatgt tgcttgcttt gaaaactatt ttttcttctg agcaaggtgt gacaagtatt
      781 atttttgatg aagtggatac aggtgtgagc ggaagagttg cacaggccat cgcagataaa
      841 atttctcaaa tatcgaaata ctctcaagtt ttgtgcatca ctcatctgcc tcaagttgct
      901 gcagtcgccg actatcaata ttatattgtg aaagcagtga ttgatggacg tacgcaaact
      961 tctgtttcag agttgaaaac aaaagaaaga gaagaagaaa ttgcacgtat gctggctgga
     1021 agtgagatta caaaactcac actcgaacat gcaaaagaac tattgaaact cgcaaaaagg
     1081 acctgagcca cctcgcagtg gttcaggtcc ttttcagcag ctgatagcta tatttta
//
LOCUS       DACENJ010000345         1135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.379, whole genome shotgun sequence.
ACCESSION   DACENJ010000345 DACENJ010000000
VERSION     DACENJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.379"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1124
                     /gene="cadA"
                     /locus_tag="IW748_002684"
     CDS             <1..1124
                     /gene="cadA"
                     /locus_tag="IW748_002684"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1105571.1"
                     /translation="LAVLLAVLPPLVVSGAAFSEWIYRALTFLVISCPCALVISVPLS
                     FFGGIGGASKIGVLVKGSNYLELLAQTETVVFDKTGTLTKGDFSIQTIDTKIDPKIFM
                     QYVASAEQFSTHPIAQSVLEGYAGPLLPTANIQEFAGEGILAEVDGKQVLAGNHKLME
                     RFEISFPSSQEIGTLLYLAIDQSYSGYLVIADTLKEDAVDALVQLKKAGVKNTVMLTG
                     DSKKIADHIGKQVGVDKIYSELLPEDKVQRLEEILQRNNKKTAFVGDGINDAPVLARA
                     DVGIAMGGLGSDAAIEAADVVIMNDQPSKIAEAIHLAKKTLKIVKQNIVFAIGIKILV
                     LALGAFGFASMGDAVFADVGVTVLAVLNAMRSLHVKNKR"
BASE COUNT          355 a          188 c          270 g          322 t
ORIGIN      
        1 gattggccgt tttattagct gttttacctc cacttgttgt ttctggagcg gcgttttctg
       61 aatggatcta tcgagcattg acatttttag tgatctcatg tccatgtgca ttagtgatct
      121 ccgtacccct tagtttcttt ggcggtatcg gaggagcaag taaaatcggt gtattagtaa
      181 aaggttcaaa ttacttggag ttgcttgcac aaactgagac agtggtattc gataaaaccg
      241 gcacattaac gaaaggtgac ttttcgatcc agacgatcga tacaaaaatc gatccaaaga
      301 tattcatgca atatgttgcg agtgcagaac agttttctac acatccgatt gctcaatctg
      361 ttttagaagg ctacgcaggt ccgcttttac caacagctaa tatccaagaa tttgcaggtg
      421 aagggatctt agctgaagtt gatggcaaac aagtattagc aggtaatcac aaattgatgg
      481 aaagatttga aatctctttt ccttcaagtc aagaaatcgg tacattgctt tatctagcga
      541 tcgatcaaag ttacagtggt tatcttgtta ttgcagatac cttgaaagaa gatgccgtag
      601 acgcattggt tcaattaaaa aaggctggtg tgaagaacac agtcatgttg acgggcgatt
      661 cgaaaaaaat tgctgatcat atcgggaagc aagtaggcgt ggacaagata tatagcgaat
      721 tgcttccaga agataaagtg cagcggttag aggaaatctt acaaagaaac aataaaaaga
      781 ccgcatttgt cggagatgga atcaacgatg caccggtgct agcaagagca gatgtaggaa
      841 tcgcaatggg tggattggga agcgatgctg caattgaagc agcagatgta gtcatcatga
      901 atgatcaacc atcgaagatt gcagaagcaa tccatttagc aaagaaaaca ttgaaaatcg
      961 tcaaacagaa cattgtcttt gctattggga taaaaatttt ggtacttgcc ttaggtgctt
     1021 ttggttttgc atcaatggga gatgcagtat ttgcagatgt tggggtgaca gtattagctg
     1081 ttttgaatgc catgcgaagc ttgcatgtaa aaaataaacg ttgatcaatg aagag
//
LOCUS       DACENJ010000346         1115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.382, whole genome shotgun sequence.
ACCESSION   DACENJ010000346 DACENJ010000000
VERSION     DACENJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.382"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..1076
                     /locus_tag="IW748_002685"
     CDS             6..1076
                     /locus_tag="IW748_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1105572.1"
                     /translation="MAQHQVELAYISDPGHIAYFSGYESDPHERVLALFIPLEQDPFL
                     FTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRTRYGQPGNVGIEKNALTVERF
                     DAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAFEIGFKAVKEGVT
                     EQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISPNELVLFDLGVVW
                     NGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGELDQIARNVINSY
                     GYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYIPEKVGVRIEDCV
                     YVTSDGCVPFTTTPKELLVLEK"
BASE COUNT          338 a          244 c          245 g          288 t
ORIGIN      
        1 aatggatggc acaacaccaa gtcgaattgg cttatatcag cgaccctggc cacatcgctt
       61 acttttccgg atacgaaagt gatcctcatg agcgtgtact agctcttttt atccctcttg
      121 aacaagatcc tttccttttc actcctgctt tggaagttga agatgctcaa aacagtagtt
      181 gggcgtatga tgtccgcggc tatttagaca gcgaaaaccc ttgggagatc atcggaaaag
      241 aacttcgtac tcgctatggt cagcctggca atgtagggat tgaaaagaac gccttgactg
      301 tcgagcgctt cgatgctatc tctgatatct tgacgggaac ggattcttac actgatctaa
      361 cttccatcat ccaacgtcta caactgatca aaactctaga ggaaaaagac aaactgatcg
      421 aagctggtaa atgggccgat gttgcatttg agattggctt taaagcagtg aaagaaggcg
      481 tgactgaaca agcgatcatt gcagaaattg aataccaatt gaaaaaacaa ggtgtttcac
      541 aaatgtcttt cgacacatta gtccttgcag gagcaaatgc tgcaagccct catggtacac
      601 caggaaatac gaagatttca ccgaatgaac ttgtgttatt cgatcttggc gttgtttgga
      661 atggctattg cagtgatgct actcgtacaa ttgcttatca aaaaccaacc gacttccaag
      721 aaaaaatcta caacatcact cttgaagcac agcttgccgc acaagaagcc gtccgtccag
      781 gtgtgacagc aggtgaactt gatcaaatcg cacgaaacgt gatcaacagc tatggctacg
      841 gcgaatattt caatcaccgt ttaggtcacg ggatcggaac aaccgtccac gaattccctt
      901 cccttgtaga aggaaatgac ttagtaatcg aagaagggat gtgcttctct cttgaaccag
      961 gaatctatat tcctgaaaaa gtaggggtac gtatcgaaga ctgtgtatat gtgacaagcg
     1021 atggctgtgt gccattcacg actacaccaa aagaattact tgttttagaa aaataagagt
     1081 aaaaaaggtg agagctagtc cgctcccacc ttttt
//
LOCUS       DACENJ010000347         1115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.383, whole genome shotgun sequence.
ACCESSION   DACENJ010000347 DACENJ010000000
VERSION     DACENJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.383"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(74..1030)
                     /locus_tag="IW748_002686"
     CDS             complement(74..1030)
                     /locus_tag="IW748_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1105573.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
BASE COUNT          337 a          226 c          199 g          353 t
ORIGIN      
        1 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
       61 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
      121 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
      181 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
      241 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
      301 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
      361 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
      421 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
      481 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
      541 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
      601 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
      661 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
      721 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
      781 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
      841 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
      901 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
      961 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
     1021 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
     1081 tatcagacaa aagtttaggt atgatagtaa aaaga
//
LOCUS       DACENJ010000348         1114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.384, whole genome shotgun sequence.
ACCESSION   DACENJ010000348 DACENJ010000000
VERSION     DACENJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.384"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            42..713
                     /locus_tag="IW748_002687"
     CDS             42..713
                     /locus_tag="IW748_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1105574.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            772..>1114
                     /locus_tag="IW748_002688"
     CDS             772..>1114
                     /locus_tag="IW748_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105575.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFE"
BASE COUNT          385 a          171 c          214 g          344 t
ORIGIN      
        1 aaatatgcta ttttaaaaat gatgaaaaaa gggggagaaa aatgaatttt ttagttgttt
       61 taaaagatac tctgattgag cgatctacat ttctatatgt tcaattgcag gaaaataaaa
      121 cattgctgca tttttcacca gagttccatc tagaggggct aacattagga gaaatctatc
      181 aagcagaagg gctgtcggct gttttgcact tttttaaggt agaattggaa ttaccagtaa
      241 aagattatat cgagctttct cttgaatctt gggatcgagc agtggttgaa ctgtttccag
      301 aaggtttaat gatcgataca aacgatggga agatccatct gactgcagca gaactgcaaa
      361 aacaaatgcg ttatcaaata gatgacgaaa attctttttc tatttttaga cgtcagcaaa
      421 agattctaaa aagcttttta aaagtgatca gtaaaaaacg gaatctgatc aaaacgacac
      481 agctgttgaa aaaatatcca ggtctgcttc acacgtctat tcaatttcct caattgatta
      541 cgatgatcca tcgttttata cgtatgcaac agctaccatc taagaagctg tttcttccat
      601 tgacggacac atttcgagta gtcaacagag aagataaaaa gaaagtgatt gtgattgact
      661 ttaccagaaa tcggaatgtc cttcaccaag ttgcaatgga aaaggccaat taaggctttt
      721 tctttttgga tttttttgtc ataataaata ttagatgatg gaggagataa tatgatgcaa
      781 acatggttag aaaggctaat acgatttcag cagaactatc acaaacttat gcagggaaga
      841 tatgcgaaat ttgatcagtt gaatcgcggt ctattgatcg ctgcgcttgt ctttagtttg
      901 ctaagtcgtt ggttgcccta tagaatcgga caattgttgt tcatcgtctt tttcggatgg
      961 atgatatttc gtttcctatc aaaaaaaatc taccccagac taaatgagaa tcaacgttat
     1021 ttgaaatatc ttgatagagt caaagaaaaa tatcatcaga taaaaaacaa aaagcaagtc
     1081 aaaatggatc gaaaaagtta tacttttttt gagt
//
LOCUS       DACENJ010000349         1096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.386, whole genome shotgun sequence.
ACCESSION   DACENJ010000349 DACENJ010000000
VERSION     DACENJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.386"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..630)
                     /locus_tag="IW748_002689"
     CDS             complement(<1..630)
                     /locus_tag="IW748_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1105576.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFI"
     gene            complement(680..889)
                     /locus_tag="IW748_002690"
     CDS             complement(680..889)
                     /locus_tag="IW748_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105577.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
BASE COUNT          315 a          283 c          196 g          302 t
ORIGIN      
        1 gatgaaccgt tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc
       61 agtcgccaca tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg
      121 ttcaggatct tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac
      181 gtaggcaacc agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta
      241 atcgattgct cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca
      301 tgaactatcg taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat
      361 ttgttctaac aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt
      421 atgcccgcta aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg
      481 tgctgcttgg gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg
      541 tttcaagcac aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt
      601 cacaaaggaa tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc
      661 ccgcagttca gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat
      721 gttcagcatc cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg
      781 ctgccgtccc aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca
      841 gtaaataggc ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat
      901 catcgtccca tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa
      961 acaagcaaaa gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa
     1021 agtcaaaggt gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat
     1081 cagtcctttt gagcat
//
LOCUS       DACENJ010000350         1088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.388, whole genome shotgun sequence.
ACCESSION   DACENJ010000350 DACENJ010000000
VERSION     DACENJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.388"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..523
                     /locus_tag="IW748_002691"
     CDS             <1..523
                     /locus_tag="IW748_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1105578.1"
                     /translation="EELTTLIKEAIKESPTAFNAQSTRAVILFGDAHEKLWEITEEAL
                     RPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTDVVKGLQEQFELYADNFPDWSEQSNG
                     IATANTWVALVDKGLGANLQHYNPVIDEAVAKEWNIPSNWKLRSQLVFGSPETPAGEK
                     EYMNDADRFRVFG"
     gene            complement(581..1063)
                     /locus_tag="IW748_002692"
     CDS             complement(581..1063)
                     /locus_tag="IW748_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105579.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
BASE COUNT          383 a          223 c          183 g          299 t
ORIGIN      
        1 tgaagaatta acaactttga tcaaagaagc aattaaagaa agcccaactg ctttcaatgc
       61 tcaatcaaca cgcgcagtta tccttttcgg agatgcacat gaaaaacttt gggaaatcac
      121 tgaagaagca cttcgcccat tgactccagc agaagctttc ccaaatacac aaaacaaatt
      181 agctggtttc aaaaatggtt atggtacagt attattcttc aaagatactg acgtggtaaa
      241 aggtctacaa gaacaatttg aattatatgc agacaacttc ccagattggt cagaacaatc
      301 aaacggaatc gcaacagcta atacttgggt agcattagtt gataaaggat tgggcgcaaa
      361 cttgcaacat tacaacccag ttattgacga agcagttgca aaagaatgga atatcccttc
      421 aaactggaaa ctacgttcgc aattagtatt cggttcacca gaaacaccgg ctggggaaaa
      481 agaatacatg aacgatgccg atcgcttccg cgtatttgga taattttatt tgaaataaaa
      541 aacagaggtt gtgacaaaca tcttgtcgca acctctgttt ttattctgga tcaatcataa
      601 tatcaatctc tgcctcatcg tttaagcgtt gggacaaaaa tggaactaat tcttctaatg
      661 atgcagaaaa tttcagcatc ggtcctcgcc atccgtttcg gaaaaaattc atttttacga
      721 ttttagaacc ttttggctga tataatacaa ttttattgat ctcagaaaaa ggaacacctt
      781 ttttgtcaaa gctattcaaa acaagtcctt cttctgtgat tcctctactg tccaacaaaa
      841 aactaataaa aatcattgct gacaagatcc cttttgccaa ttcatctgct gtaggatgac
      901 tgatgagtgt ataccaaatg actagtgaca ctaagaaggg tatcaccaag tagacccatc
      961 gatttcgcac agtccgataa ataatttctt ttcgaaccag ccaaagaaat aaaaacatca
     1021 caacaacgaa taaaaacaat agaaccgtaa agaatgttac catgacaact cgcctcgctt
     1081 tttcttct
//
LOCUS       DACENJ010000351         1076 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.390, whole genome shotgun sequence.
ACCESSION   DACENJ010000351 DACENJ010000000
VERSION     DACENJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.390"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(53..451)
                     /locus_tag="IW748_002693"
     CDS             complement(53..451)
                     /locus_tag="IW748_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1105580.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(448..684)
                     /locus_tag="IW748_002694"
     CDS             complement(448..684)
                     /locus_tag="IW748_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1105581.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(844..>1076)
                     /locus_tag="IW748_002695"
     CDS             complement(844..>1076)
                     /locus_tag="IW748_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105582.1"
                     /translation="YGTKLLSLTHNQTAYSPTLILSTLTAGAKSATYFFHKTTSMLKR
                     KKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT          333 a          191 c          167 g          385 t
ORIGIN      
        1 atattcctta atgacatgct gatctaattg agaaataaag tttttaattc gttcatttta
       61 ttgcggtttg tcgtaaataa ctggttatct tggactttag tgaatcgaaa tccaatgaac
      121 tagtggtaat cattatgata aaatcaacag cttcttgacg agaaaaagca actggacaat
      181 tattcaattg aagaaacaac aacatggcaa caagagcagt tcgtttattg ccattatgaa
      241 aaggatgttt tttagctaaa ttaattgcta aaatcgccgc tttttcatag atttcaggat
      301 agagttcttg attgaatact gcttgtgaag gctgtttgac ggccatatct aatgcatttg
      361 catcttttac accgatcatt tcgcctggcg agtgacgtaa aataactttc gcgttgattt
      421 tgataatttc tttagtcgaa agatacctca taaatcaacc aaatctttta acgcgtcttc
      481 atattgattc atgatcgaat caactaatga atcaatatca ggttcttctt gtgctttgat
      541 cattcgtaca gaaccatcag attgaaaaac ataactgatt gcgtcacctg tctgcacacc
      601 catgaattct tttacttctg gtgggatagt tgttacatcg ctattgccag attttctaat
      661 tttatacttt ttcatttttt tcatatttcg ccaccctcta taccattctc attctatagt
      721 aagtacatta tatccaagta tatactttta atcaatgtat atacttttat tttgcttttt
      781 cttacgaaaa aatgacaaag aaaaaagttt gtgacatgac ttttgtcaca aactctcgta
      841 ttcttaacca aaatattgat gaaatacttg tgtaataact ggtagaaaat aaataatgat
      901 catagccgct accaccacca acaattgttt tcgctgtttc tttcgcttta gcatgcttgt
      961 cgttttatga aaaaaataag tagcgctttt agctcctgca gtcaaagtcg ataaaatcaa
     1021 cgttggtgaa tatgctgttt gattgtgcgt caatgacaac aacttcgtcc cgtaat
//
LOCUS       DACENJ010000352         1075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.391, whole genome shotgun sequence.
ACCESSION   DACENJ010000352 DACENJ010000000
VERSION     DACENJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.391"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..138
                     /locus_tag="IW748_002696"
     CDS             <1..138
                     /locus_tag="IW748_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1105583.1"
                     /translation="YMDEHMTLSLDPRLGVFTLESPSGSLDSHVQLVDLRPEKFSGIK
                     Q"
     gene            complement(252..881)
                     /locus_tag="IW748_002697"
     CDS             complement(252..881)
                     /locus_tag="IW748_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1105584.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDIA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
BASE COUNT          350 a          183 c          184 g          358 t
ORIGIN      
        1 tacatggatg aacatatgac cctttcttta gatccacgtc taggtgtatt tactcttgaa
       61 agcccaagtg gttctttaga cagtcatgtt caattagtag atctacgacc agaaaaattc
      121 tctggaataa aacaataact ttctctctat tgtccataaa actagtctca atcaagctgt
      181 attgaaaaaa caagacaaca ttcttaccat ggtaaataaa aagacgacac attttgtcgt
      241 ctttttttaa attaactatt ttcgataaaa gatcgcatag cttgtgccat tatttctggc
      301 ttttctctat tcaattcatg ttttccgttt ttaataaaga gtagccggcc attcgttgaa
      361 agtttatata aacgttttgc tgctggttta ttcgcatgat ctttggtacc gcataaaatc
      421 aatttaggaa gtcgtgatgt acgaagctgt tttttgcagt ctaatgtttc aagtgaacga
      481 agtagggtaa gggtttgttt tttagataga cctaattttt caaatatctt agaaggtacg
      541 cagtaaaaat aaatattctg gaaagtaagc aataagcgat taggagactc aaattgtgga
      601 gcgactaaaa caaccttttt taaatatgga gaaggagttg caagatagtt taaagcaata
      661 acagcgccta aagataatcc acataaaata aaaggttcct ttatctgatt caattgtact
      721 tcgatgtatt tggttaagtc gtctaaagta gcaatgtctg tgctgtttaa atgattgaac
      781 aatgaaagac ttttgcaaga ataactattg ggtagataag agattacatc tttccatgat
      841 tgttcgtctt ggcctagacc atgcaaaaaa acaattttca tattgctctc ctttttatag
      901 ttatcatata gactttcttt gtactattaa aatataaaca ttcatttctt acgtcataaa
      961 ataaaaaaac agcgcagaca cattgttaaa ccaacatgtt gcactgttta aggaagccac
     1021 ttgccgggct tgaaccggcg acctcttcct taccatggaa gcgctctacc agctg
//
LOCUS       DACENJ010000353         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.393, whole genome shotgun sequence.
ACCESSION   DACENJ010000353 DACENJ010000000
VERSION     DACENJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.393"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            95..1054
                     /locus_tag="IW748_002698"
     CDS             95..1054
                     /locus_tag="IW748_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HAQ1105585.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
BASE COUNT          373 a          216 c          211 g          262 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aa
//
LOCUS       DACENJ010000354         1046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.395, whole genome shotgun sequence.
ACCESSION   DACENJ010000354 DACENJ010000000
VERSION     DACENJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.395"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..390
                     /locus_tag="IW748_002699"
     CDS             <1..390
                     /locus_tag="IW748_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105586.1"
                     /translation="LNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELYKEFG
                     DLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNAIS
                     IKDLKELLNSKRNELKQIKETNSVELE"
     gene            404..>1046
                     /locus_tag="IW748_002700"
     CDS             404..>1046
                     /locus_tag="IW748_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105587.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYY"
BASE COUNT          371 a          143 c          225 g          307 t
ORIGIN      
        1 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
       61 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      121 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      181 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      241 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      301 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      361 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      421 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      481 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      541 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      601 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      661 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      721 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      781 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      841 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
      901 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
      961 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1021 gacaatgaat ataggtattt attaca
//
LOCUS       DACENJ010000355         1045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.396, whole genome shotgun sequence.
ACCESSION   DACENJ010000355 DACENJ010000000
VERSION     DACENJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.396"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..>1045)
                     /gene="uidA"
                     /locus_tag="IW748_002701"
     CDS             complement(23..>1045)
                     /gene="uidA"
                     /locus_tag="IW748_002701"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ1105588.1"
                     /translation="DNKVIDTYTEEFGIRSVQVTNGKFLINNKPFYFKGFGKHEDSYV
                     NGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYSEEIMRLADREGIVVIDEVPAVGLHL
                     NFMATLLDDNVEKHNTWKEIRTENSHKTVIKELIERDKNHPSVVMWSIANEPDSDSEG
                     AKEYFEPLVELTKELDPQKRPVTIVTYLKSTPDICKVGDIVDVLCLNRYYGWYVSGGD
                     LAHAKFLLKKELDGWKERCPDTPIMFTEYGADTVAGFHDTVPTMFTEEYQVAYYEANH
                     EVIDTCENFVGEQVWNFADFATSQGILRVQGNKKGIFTRDRKPKMIAHYLQKRWSSIP
                     NFSYKK"
BASE COUNT          319 a          220 c          129 g          377 t
ORIGIN      
        1 aacattctta aaaaaatcca aattattttt tataactgaa attgggtatt gatgaccatc
       61 gtttctgtag atagtgagca atcattttag gttttctatc tctagtaaag attcctttct
      121 tatttccttg cactcttaaa atcccttgac tcgttgcaaa atctgcaaaa ttccacactt
      181 gttcaccaac aaaattttca catgtatcta ttacttcgtg atttgcttcg taataagcca
      241 cttgatactc ttctgtaaac attgttggta ctgtatcatg aaatccagct actgtatctg
      301 ctccatattc tgtaaacatt attggcgtat caggacatct ttctttccat ccatctagct
      361 cttttttcaa cagaaatttt gcatgtgcca gatcccctcc cgaaacatac caaccataat
      421 aacgatttaa acataaaaca tcgacaatat ctcctacctt acaaatatct ggcgttgatt
      481 ttagataagt aactattgtt acaggtcttt tctgcggatc aagttcttta gttaattcaa
      541 ctaaaggttc aaaatattct ttagcacctt cggaatccga atctggttca tttgcaatag
      601 accacattac aacacttggg tggttcttgt cgcgttctat caattccttg atcaccgttt
      661 tatgagaatt ttctgttcgt atctctttcc acgtattatg tttttccaca ttatcatcta
      721 ataaagtggc catgaaattt aaatgtaacc ctactgcagg tacttcatca ataactacaa
      781 taccttctcg atctgctaat cgcataattt cttctgagta aggataatgt gatgttctaa
      841 aagaattagc acctatccat ttcattaaat taaaatcttt aatatttaca gcctcattca
      901 aaccacgtcc attaacatac gaatcctcat gcttaccaaa tcctttaaaa taaaaaggtt
      961 tattattaat caaaaatttg ccattggtaa cctgaacact gcgaatacca aattcttctg
     1021 tataggtatc gatcacttta ttatc
//
LOCUS       DACENJ010000356         1044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.397, whole genome shotgun sequence.
ACCESSION   DACENJ010000356 DACENJ010000000
VERSION     DACENJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.397"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..398
                     /locus_tag="IW748_002702"
     CDS             <1..398
                     /locus_tag="IW748_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1105589.1"
                     /translation="TSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAAT
                     EGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNE
                     DEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            412..768
                     /locus_tag="IW748_002703"
     CDS             412..768
                     /locus_tag="IW748_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1105590.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          357 a          161 c          199 g          327 t
ORIGIN      
        1 atacttcgcc tagctgtact tcctgtagaa aagcaagagc atggttgcaa gcgaatcaac
       61 ttgaatttga agagaagaat atttttgccg aaccgttaac agaaagtgag atcaaaaaga
      121 tcttggcagc gactgaagga ggagttcaag atattatttc cactcggtcg aaaatttacg
      181 aaaagctggg catagatttt aacgaattaa ctttaaagca aatgatcgtc ttattcaaag
      241 aatatccttc attattgaga cgacctattt tgattgatga aaaaagaata ctaatcggat
      301 ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt gccagaaatc
      361 tgatctattt acaaaacaat aacatcttaa attcataggg agggagcctt cgtggaagtg
      421 aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg gttagaacac
      481 catcaattgc cttatcaatt tgttgactta gaagaacgcg atctaagtac agaagaagca
      541 gaagaattat gcagttttga agaagtgcaa gcagaacagc tatttgcgac ttggtctgag
      601 gcttttcaca acattgcgat tgacctttcc tgtgtaggaa aaaaacaact gattttttta
      661 tgcacacaac ataccaatct cctcagacgt cctttgatta ttatcgatga agttctattt
      721 attggataca atgagcagtt gatggaacag cttatcttaa ataaatagac tttaactgtt
      781 ttaatcaagt cactgagcat ttcatacagt tgtaacagtc gaacacttag catcaagcgt
      841 gttatgtgtt ctttttttga ctttattttt ctactataga gacatgctca atttatgtgg
      901 tcaaaattca aatctaggtg gggatataga gttaaaaata tattgacatg tgtaaattta
      961 catgatatat tcatttctgt tgttttaagt agcaaagcaa taaaatagta ttttaaaaaa
     1021 catataaaat agttgttgac atgt
//
LOCUS       DACENJ010000357         1031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.398, whole genome shotgun sequence.
ACCESSION   DACENJ010000357 DACENJ010000000
VERSION     DACENJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.398"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>1031)
                     /locus_tag="IW748_002704"
     rRNA            complement(<1..>1031)
                     /locus_tag="IW748_002704"
                     /product="16S ribosomal RNA"
BASE COUNT          219 a          323 c          229 g          260 t
ORIGIN      
        1 ggggcatgat gatttgacgt catccccacc ttcctccggt ttgtcaccgg cagtcttgct
       61 agagtgccca actgaatgat ggcaactaac aataagggtt gcgctcgttg cgggacttaa
      121 cccaacatct cacgacacga gctgacgaca accatgcacc acctgtcact ttgcccccga
      181 aggggaagct ctatctctag agtggtcaaa ggatgtcaag acctggtaag gttcttcgcg
      241 ttgcttcgaa ttaaaccaca tgctccaccg cttgtgcggg cccccgtcaa ttcctttgag
      301 tttcaacctt gcggtcgtac tccccaggcg gagtgcttaa tgcgttagct gcagcactga
      361 agggcggaaa ccctccaaca cttagcactc atcgtttacg gcgtggacta ccagggtatc
      421 taatcctgtt tgctccccac gctttcgagc ctcagcgtca gttacagacc agagagccgc
      481 cttcgccact ggtgttcctc catatatcta cgcatttcac cgctacacat ggaattccac
      541 tctcctcttc tgcactcaag tctcccagtt tccaatgacc ctccccggtt gagccggggg
      601 ctttcacatc agacttaaga aaccgcctgc gctcgcttta cgcccaataa atccggacaa
      661 cgcttgccac ctacgtatta ccgcggctgc tggcacgtag ttagccgtgg ctttctggtt
      721 agataccgtc aagggatgaa cagttactct catccttgtt cttctctaac aacagagttt
      781 tacgatccga aaaccttctt cactcacgcg gcgttgctcg gtcagacttt cgtccattgc
      841 cgaagattcc ctactgctgc ctcccgtagg agtttgggcc gtgtctcagt cccaatgtgg
      901 ccgatcaccc tctcaggtcg gctatgcatc gtggccttgg tgagccgtta cctcaccaac
      961 tagctaatgc accgcggttc catccatcag cgacacccga aagcgccttt caaatcaaaa
     1021 ccatgcggtt t
//
LOCUS       DACENJ010000358         1028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.399, whole genome shotgun sequence.
ACCESSION   DACENJ010000358 DACENJ010000000
VERSION     DACENJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.399"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(53..787)
                     /locus_tag="IW748_002705"
     CDS             complement(53..787)
                     /locus_tag="IW748_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1105591.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          363 a          211 c          183 g          271 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaa
//
LOCUS       DACENJ010000359         1025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.400, whole genome shotgun sequence.
ACCESSION   DACENJ010000359 DACENJ010000000
VERSION     DACENJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.400"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..909
                     /locus_tag="IW748_002706"
     CDS             <1..909
                     /locus_tag="IW748_002706"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1105592.1"
                     /translation="VIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTITAYT
                     SGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSYFGP
                     LTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKYNEI
                     QPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQLQEK
                     LYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKLLEK
                     GGMPMLEKYAKLHFANTEKALKLLKK"
BASE COUNT          364 a          207 c          196 g          258 t
ORIGIN      
        1 gtcattatca aagcaacaaa cgaccaactt agtcaaatga aagcatacta tcgctcttat
       61 ttgttaaata aacagattcc ttatactagc tttgctgcaa aaaaagacgg gacgaccatt
      121 accgcttata catccggaaa agtgatgttt caaggtacga atgcagcaat agaagctgat
      181 aaatgggggg aggcacagga taaacaagcc aagccagcaa aaacagcctc ttctttgcca
      241 aaaaatatca gtcaattatc cgttttaggt agtgatgagg tcggaaatgg cagctatttt
      301 ggtccgctaa cagtatgtgc tgcttatgtg aaacgtgatc agcttgcaga attacgtaaa
      361 cttggtgtta gagattcaaa agagttaaaa gatagccaaa tcatccagct tgctgacatc
      421 ttaaaaaaaa ccatccccta taaactgctt gtcttatctc cagaaaaata caatgagatc
      481 caaccaaaat acaatgccgt tcggatgaaa gtcgctttgc acaatcaagc gatccatttg
      541 ctgctaaaaa aaatcgagcc agaaaaacca gaagcgattt tgatcgatca gtttacgccg
      601 gaagcaaatt ttaaaaaata tgcgcgttta gaaaaaaatc aattacaaga aaaactgtac
      661 ttcgttacta aaggagaaca atatcattta gctgttgctg ctgcttctat catcagtcgt
      721 gccagctttt tggaagagtt acacaaagca tcagctgaag ctggaatcac ccttccatca
      781 ggagctggaa cgaaatctga ccaagtagcg gcaaaactat tggagaaagg cggcatgcct
      841 atgcttgaaa aatatgccaa acttcatttt gccaatacag aaaaagcttt gaagctgcta
      901 aaaaaataat ggaactgtga caaaagtcat gtcacagtcc cttgttccga ataaacggtg
      961 ttctcatgcc aactcctcgg tcacaagtca caatagtcat caaacatgag aagctgtttt
     1021 ttgaa
//
LOCUS       DACENJ010000360         1012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.403, whole genome shotgun sequence.
ACCESSION   DACENJ010000360 DACENJ010000000
VERSION     DACENJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.403"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            20..979
                     /locus_tag="IW748_002707"
     CDS             20..979
                     /locus_tag="IW748_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1105593.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          341 a          184 c          209 g          278 t
ORIGIN      
        1 ggctcttttt ttattgccaa ttaacggatt tgcctatact atcaacaatg aatttaattt
       61 gggcgcaaat gaaggtagct cacaagtagc aaataatcag tatattttac tgcatgaaac
      121 ggctaatgaa acagcaacag gacgcaatga agcgcagtat atgcaacgtt catggactag
      181 cgcttatact gcttatattg tgggagacgg cggaattgtt tatcaagtcg gtcaacctgg
      241 ttatgtacag tacggtgctg gttcgtatgc taatgccaac agtcctgtgc agattgagtt
      301 acaacacaca catgataaag caacgtttga gaaaaactac aaggcatacg ttgaattggc
      361 tagagattca gcaatgaaat atggtattcc attaacgttg gacactcctt ataaccaacc
      421 gggaatcaaa tcgcatttat gggtaacaca aaacatctgg ggcgatcata cagatcctta
      481 cggttatctt tctgaaatgg gcgtaagtaa agaaaaatta gcatatgatt tagctcatgg
      541 atttaccgat gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc
      601 tggtgcagct aatcctacac tgacagatgg aacgaattac gcccacattg atcagtttgg
      661 agaaatcgaa aatgcaaatt tgcatgtagc tggatggcac attgctaact ataaatacga
      721 gtatatcttc attatggatt acaatactgg gaaagaatta gctcgagtaa gagctgatgg
      781 aatttataga tcagatgtaa atcaagctta taatacttct ggaaatgttg gctatcatgt
      841 atcttttaac atgcgtaact tccctaataa gaaagtctat gtcatgatgc gggcaacgaa
      901 tgatccagag ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt
      961 aaatattcct aaacgataaa aatagctcct cgttgaggag cattacataa ct
//
LOCUS       DACENJ010000361          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.409, whole genome shotgun sequence.
ACCESSION   DACENJ010000361 DACENJ010000000
VERSION     DACENJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.409"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>943
                     /locus_tag="IW748_002708"
     rRNA            2..>943
                     /locus_tag="IW748_002708"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACENJ010000362          889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.414, whole genome shotgun sequence.
ACCESSION   DACENJ010000362 DACENJ010000000
VERSION     DACENJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.414"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            18..>889
                     /locus_tag="IW748_002709"
     CDS             18..>889
                     /locus_tag="IW748_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1105594.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLI"
BASE COUNT          281 a          176 c          171 g          261 t
ORIGIN      
        1 tcaaaggagc gttctgtatg cctgatcttc atcatatcaa actactactt ggaatcaaag
       61 ataaaaacat tttcatcaca aatgtggaat cgaagagcat taagaatatc aagagtcttg
      121 ttgtttctgc gacgttaagt aaagaaattc gtcgctgtcc tttgtgtaaa cagatgaatc
      181 atgaaggcat gatcgttaag aacggaaaga aaaaatcgct cattcaactt aataaatgtg
      241 cgaaccaact gacttatctt gctttagcga aacaacgcta ccactgtcgt ggctgccaca
      301 cctattttac ggcgaatacc tacattgtcg atagaaactg ctttatcgct aaacaagtgc
      361 gctataaaat tttagaagag ctaactgaaa aacaagcaat gacgacaatt gctaagcatt
      421 gcggcgtttc ttggtcgacc gtttctcgga cactcgcttc tctgatcccc atgacaaaag
      481 taaaaagaaa ttggttaccg cgttgtttat taatggatga atttcgttca ctgaaaaatc
      541 aagtcggtcc ctactccttt tcttgtatgg atggcgatac tggaaaatta ctggatattc
      601 ttccttcacg gaagaaaaag gctctcgttt cttattttat gcaatttgaa cgacgcgcac
      661 gattgaaggt aaaggtcatc gtgacggaca tgaatgtttc ttatgcctct ctgataaagg
      721 aatgttttcc aaaggcaaaa ctcgttgtgg atcgttttca cattgtcaaa catctcattc
      781 gtaaatttga ggatattcgc gtacgaatca tgaaaagctt tgatcgaaac gatccaacac
      841 aagccaaata ttatcgacaa ttaaaggcgt tgtctcggct tctgatcaa
//
LOCUS       DACENJ010000363          888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.415, whole genome shotgun sequence.
ACCESSION   DACENJ010000363 DACENJ010000000
VERSION     DACENJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.415"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            310..426
                     /locus_tag="IW748_002710"
     CDS             310..426
                     /locus_tag="IW748_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1105595.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT          305 a          149 c          145 g          289 t
ORIGIN      
        1 cttggagtta aacgcttctg tcccagcctc ttttttagtg ttctatcgaa cacacagtac
       61 gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa gagggccttt
      121 tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt caatcagcta
      181 gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc atttaaaaca
      241 caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag aagaaagtgg
      301 gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa aagcccaaat
      361 atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa
      421 aggtaagaag aaggaaactt aattaaaaaa atagccgaaa aacgtttttc ggctattttc
      481 aataatcggt ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc atttttaaca
      541 tatgataaat tggtagaatc tttcttttca aattgatacc attccctaat tccagttata
      601 tagttaacta cagaaccaca aagcacgtac gtttataaaa gtattatctg tagttatttt
      661 tcaattccct ttcaacctct tattttgacc agtagtattg atcactttac cttttaacac
      721 ctttgttcgt ttttgaaaca tttttagtca agaatggaaa cgtaactaca taatcaaagg
      781 ttttgcgaaa tatttcatga aatatttcat gtagaagcga gcaatacggt tgcaaataac
      841 cattatcgtt taataagcag ttacgaaagc tcccaatatc tctaacgt
//
LOCUS       DACENJ010000364          871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.417, whole genome shotgun sequence.
ACCESSION   DACENJ010000364 DACENJ010000000
VERSION     DACENJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.417"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            92..>871
                     /locus_tag="IW748_002711"
     CDS             92..>871
                     /locus_tag="IW748_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ1105596.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALED"
BASE COUNT          321 a          126 c          175 g          249 t
ORIGIN      
        1 ataagaatac ctgtattacg tgaagaatcg aaaaaattga aatcgatttt taatgacatt
       61 atgcttatag aagaaagtga ggtaatatct tatgaataaa caagagttgc atgattttat
      121 tggttttcga caaccaaata tatgtcaaat tgtcgcatat aaggatgggg tagaagttta
      181 tacagatgaa tggaatggtt ataaaaaaga cgatacttgt catatcatgt cagcaacgaa
      241 aagtattgta tctttgctga ttggcattgc tttagagcaa ggattaatca aaagtatcga
      301 tgataaagta ttagattatt ttccagagta taaagtgaaa cgcggggaga aaactattta
      361 cgctattaca atcaagcact tgctaacaat gaaagcaccc tacaaatgta aaggcgatcc
      421 atggacaaaa gtctgttcaa gtgaagattg gacaaaaaca tcactagatt tattgggagg
      481 aagaaaaggt ataaccggcg aattcaacta tcaaactgtc tgtttacaca tattaactgg
      541 cttgttgtat cagacaagta agctaacaac tgttgatttt gccaataagt atctatttga
      601 tcctataggg ataccaaacc atgtcaatta ttatgctgaa acagctgaag aacataagca
      661 gtttacaatg gataagtctc ctaaaaccaa tgtctggttt tgtgatccac taaatatcgg
      721 gacggcagga tatggtttgt gtttatcggc aagtgatctg gcaaaaattg gtgtattatg
      781 cctaaataaa gggatgtata aaggaaaaca aatcgtacca cccatgtgga taaacgagat
      841 gttcaaacct agaaccagtg ctttagaaga c
//
LOCUS       DACENJ010000365          869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.418, whole genome shotgun sequence.
ACCESSION   DACENJ010000365 DACENJ010000000
VERSION     DACENJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.418"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            209..865
                     /locus_tag="IW748_002712"
     CDS             209..865
                     /locus_tag="IW748_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1105597.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
BASE COUNT          313 a          125 c          162 g          269 t
ORIGIN      
        1 tcatccagta attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa
       61 cgaataaaaa aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta
      121 atatcattag taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt
      181 agatgaaagt ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga
      241 taaaaatgat tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat
      301 ggaagattcc catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa
      361 tgatgggaga gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa
      421 agacgaatat tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc
      481 agagttttat cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct
      541 acaacactat gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca
      601 gcaaatgcta ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga
      661 tttattcggt gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga
      721 aatcgccaaa ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact
      781 gaaaaaagat ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt
      841 gttagaagac aatattatct tttgatgaa
//
LOCUS       DACENJ010000366          854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.422, whole genome shotgun sequence.
ACCESSION   DACENJ010000366 DACENJ010000000
VERSION     DACENJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.422"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(102..680)
                     /locus_tag="IW748_002713"
     CDS             complement(102..680)
                     /locus_tag="IW748_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1105598.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT          262 a          147 c          149 g          296 t
ORIGIN      
        1 ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa
       61 aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct
      121 atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta
      181 tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt
      241 gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga
      301 ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa
      361 ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc
      421 tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat
      481 gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt
      541 aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca
      601 tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat
      661 aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct
      721 ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa
      781 aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag
      841 aatcaaatca tatg
//
LOCUS       DACENJ010000367          824 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.425, whole genome shotgun sequence.
ACCESSION   DACENJ010000367 DACENJ010000000
VERSION     DACENJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.425"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..809
                     /gene="purR"
                     /locus_tag="IW748_002714"
     CDS             24..809
                     /gene="purR"
                     /locus_tag="IW748_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1105599.1"
                     /translation="MTQYLLNHPHELISLTSFAERYESAKSSISEDLGIIKKTFKERG
                     YGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGYVYLSDLLGEPE
                     LLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVRRDSKITEGSTV
                     SVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSLIEEFESELVGI
                     TVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
BASE COUNT          262 a          145 c          185 g          232 t
ORIGIN      
        1 gtcgcagtga acgtctagtt gacatgacac agtatttatt gaatcacccg catgaattga
       61 tttccttaac aagctttgcc gaacgttatg aatctgctaa atcttctatc agtgaagatt
      121 tgggaatcat caagaaaaca tttaaagaac gagggtatgg aaccttggaa acaatcccgg
      181 gtgctgcagg tggtgtattg ttcatccctg aaatttcata tgaagaggcg aaaaaatata
      241 ttgaatctct agcagaacgt ctatcagaac aagaccgtct gcttccagga ggatatgttt
      301 atctctctga tttgttagga gaacctgaac tgctgcgtca agttggaaga atcattgctt
      361 ctaaatacct tggtgaaaaa atcgatgctg taatgactgt agccacaaaa ggcgtaccta
      421 ttgcccaagc tgtgtcctat tatctgaatg cgccattcgt tattgtacgc cgggactcaa
      481 aaataacaga aggatcaaca gtaagtgtca actatgtgtc tggatcttct gaaagaattg
      541 aaaagatgga actgtctaag agaagtttga aacgtggttc acgtgtcctt gtagtggatg
      601 atttcatgaa aggcggcgga acagtcaacg gaatgaagag cttgattgaa gaattcgaat
      661 cagaacttgt tggcatcact gtatttgccg aatcaaaatt caacggtcat cgcgccatcg
      721 atgattatac gtcactgctt tatgtcaaag atgtagatac tcaaacgaaa aatattacgg
      781 ttgtgccagg aaattatttt tcagaataag aggttgtgaa aaag
//
LOCUS       DACENJ010000368          812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.428, whole genome shotgun sequence.
ACCESSION   DACENJ010000368 DACENJ010000000
VERSION     DACENJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.428"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(68..745)
                     /locus_tag="IW748_002715"
     CDS             complement(68..745)
                     /locus_tag="IW748_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HAQ1105600.1"
                     /translation="MLNESRLKTLHSLLNTIFHYTLGFFFIYAILSAIGVPIGSLLAG
                     AGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGDYIKLANLTIEGTVVSVGIRTLQL
                     KATDGTVHFIPNRNITTISNLSRANMQVLLDIRIVPEEGYDKIYEIIDRVNRTLAEKY
                     QEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQYALKEEFLSSYVKELTASGFTIPN
                     SPIAVGK"
BASE COUNT          247 a          166 c          155 g          244 t
ORIGIN      
        1 gacaaaagtg ttctttactt ttgtcccacg ctctttttag tgagattcgt tgcatcgttt
       61 gtccaaatta ttttccgaca gcaattggac tgttcggaat cgtgaaacca gaagctgtca
      121 attctttgac ataggagctc aagaactctt cttttaaggc atattgtttt ccgttcaatg
      181 cgtagcagac cgtacgaatc gcaaaattgc tgtttccaag atccactaat ccaaagatag
      241 aaggttcagt ttggagctct tcttggtatt tttcagctaa tgtacgattt actcgatcaa
      301 tgatctcata gattttgtca tatccttctt caggaacaat ccgaatatct aaaagtactt
      361 gcatatttgc acgagagaga ttgctgatcg tggtgatgtt tcgattcggg atgaaatgga
      421 cagtaccatc cgttgctttc agctgaaggg tacggatacc aacagatacg acggttcctt
      481 caatcgttaa gtttgctagc ttgatgtagt ccccaacatc catctgttgt tccatgataa
      541 taaaaaatcc ggtaataaca tcactcatga acccttgggc accaagaccg attgccacac
      601 ctgcaattcc ggcaccagcc aaaagtgatc cgatcggcac accgatagca gataaaattg
      661 catagataaa aaagaaacca agggtataat gaaaaatagt atttaaaaga ctatgtaaag
      721 tctttaagcg gctctcgttt agaatggctt ttttagaata ctgattaaaa gacttatcaa
      781 tcagatattt tcccacacgg tgaagaatcc aa
//
LOCUS       DACENJ010000369          795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.432, whole genome shotgun sequence.
ACCESSION   DACENJ010000369 DACENJ010000000
VERSION     DACENJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.432"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..205
                     /locus_tag="IW748_002716"
     CDS             <1..205
                     /locus_tag="IW748_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1105601.1"
                     /translation="KVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQI
                     RQQRLAKRILDNERGSDTSGEAA"
     gene            complement(235..684)
                     /locus_tag="IW748_002717"
     CDS             complement(235..684)
                     /locus_tag="IW748_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ1105602.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
BASE COUNT          231 a          164 c          131 g          269 t
ORIGIN      
        1 caaagtagaa ttcgtgcatc aataccaatt tgagacactg gcacagctac gtctagaatt
       61 gtttgattat gtgcattggt ggaactatct acgcttacat ggcacgttgg cgtatgaaac
      121 accgatccaa attcgacaac agagattggc gaagcgaatc cttgataatg agcgcggatc
      181 tgatacctct ggagaggcag cgtaattgaa tgattgtgct tctgccggag aaaatcagat
      241 ccgaggatgc tcattgtcaa gggcaatcgg agccataaca ccgcagcatt cacaacacct
      301 tataattttt gtcaaaaaaa gtgttgccat tccatctggc attaagaaag gatatgaagc
      361 ctttgtcttt tcatgtatta actttgactt ttcaattgct tccttgtttt caccgttatc
      421 atcaacagga tctgtaacaa ctccaacgat atttacaccc ttgcttttca tttcattctg
      481 aacctcaacc aaatcaggaa tttcctttac acaagctgta caccaagttg caaatacatt
      541 taccatagta agatcatact tctcaaaatc tttacttgta aaatccttgc catttatgtc
      601 ctttgttggt agtttacgta aatctttaac cgattctttg ttaaatgctt ctgtattttc
      661 taaatcagtt ttttctgata gcacaaaacc atttttagga cgttcttttt tatctataat
      721 ttgaatctca gttcttttta attcatcaag aagattactt tcagctccgc tatttgtgct
      781 taaatagcaa tcgta
//
LOCUS       DACENJ010000370          786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.435, whole genome shotgun sequence.
ACCESSION   DACENJ010000370 DACENJ010000000
VERSION     DACENJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.435"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            51..704
                     /locus_tag="IW748_002718"
     CDS             51..704
                     /locus_tag="IW748_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1105603.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          326 a           67 c          138 g          255 t
ORIGIN      
        1 ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat atgaaaatat
       61 taactgttga agatgataat atgataagag agggaataag tgaatatctt tcagaatttg
      121 gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt aatagcgata
      181 taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa gtgttgaaag
      241 aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt gatgaagaat
      301 ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca ttttcattgc
      361 cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat ttagagaagt
      421 ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata aatggtgaag
      481 aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat aataatggac
      541 aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa gaaactcctt
      601 atgatcgagt aatagatgta tatataaaag aacttagaaa aaaattacaa ttagactgta
      661 taactactat aagaaatgta ggatataaat tggagagaaa atgaaaaaat taaagatatt
      721 tccaaaaatg ttcatacaaa tattttctgt tcttggaata ataattatat tagttcattc
      781 attaat
//
LOCUS       DACENJ010000371          751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.442, whole genome shotgun sequence.
ACCESSION   DACENJ010000371 DACENJ010000000
VERSION     DACENJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.442"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            84..485
                     /locus_tag="IW748_002719"
     CDS             84..485
                     /locus_tag="IW748_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1105604.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLLEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            479..724
                     /locus_tag="IW748_002720"
     CDS             479..724
                     /locus_tag="IW748_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1105605.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
BASE COUNT          277 a          106 c          134 g          234 t
ORIGIN      
        1 tatgtttttt tctagttttt tcgaaaaaaa ggggctctct gattgttgta ttagtggaaa
       61 gggggaaaaa ggagcatatt atcatgcaac aacattacaa tgacatatct atacaacacc
      121 aatttgatgc ctactgtaaa aaagtattac gaaacgaagc taagtctatt cgaaaacgaa
      181 atgctaacat aaaggataat gaggaaccat tggcagattc aaatgaaggc aggtattccc
      241 aaactcattt tgatgaacaa gatgtctatt tcttatttgg aatggaaata ttaatttcag
      301 atcaaaagtt atcagcagca attgatcagt tattggagat aagaagaaag attatcctac
      361 tttattattt tgcaggtttt aatgatgctg aaatcggtaa aattctcaat atgagcacaa
      421 gtggtatctg gtatcaacga aagaaggcag ttgaacaatt gaaaatgggg tatgacctat
      481 ggtagataaa tccaaaaagc ggcagtacct attagttcca ctatccatta tcttatctgc
      541 tctagaagga gatccaattt cgatcaagat gatactcaaa cattatcata gttatatggc
      601 taagttatgt ttgacaaatg gatttaacga ggtaggtcaa ttcgttacct atgtagatga
      661 atatatgata agtcagctag aaattaaatt aatagaagcg atcttaaaat ttaaaattaa
      721 ctaattaaaa aaatctcctt tccacttttt c
//
LOCUS       DACENJ010000372          731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.449, whole genome shotgun sequence.
ACCESSION   DACENJ010000372 DACENJ010000000
VERSION     DACENJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.449"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            55..731
                     /locus_tag="IW748_002721"
                     /pseudo
     CDS             55..731
                     /locus_tag="IW748_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          297 a          125 c          137 g          172 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt c
//
LOCUS       DACENJ010000373          712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.450, whole genome shotgun sequence.
ACCESSION   DACENJ010000373 DACENJ010000000
VERSION     DACENJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.450"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            81..>712
                     /locus_tag="IW748_002722"
     CDS             81..>712
                     /locus_tag="IW748_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ1105606.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEET"
BASE COUNT          270 a          127 c          133 g          182 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa tccgtctcta
      481 agtccttcgt ttctagctta acagaacagc tagaacctat ggttaacgag tggcagaatc
      541 gtttattatc agaaaaaaat tatccttact taatgaccga tgtactctat ataaaagtac
      601 gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc gattggaata accaaagatg
      661 gcgaccgtga aattatcggc ttcatgattc aaagtggcga aagcgaagag ac
//
LOCUS       DACENJ010000374          705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.451, whole genome shotgun sequence.
ACCESSION   DACENJ010000374 DACENJ010000000
VERSION     DACENJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.451"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..705
                     /locus_tag="IW748_002723"
                     /pseudo
     CDS             1..705
                     /locus_tag="IW748_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
BASE COUNT          246 a          134 c          152 g          173 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaaaat tctaaatatt
      241 tctcgtcaga cctactatta tcaagcgaaa ccgatcgaaa atgagtccga cttagaagag
      301 atcgttcagg aagagtttat tcgaaaccga aaggcttacg gtacccgaaa attgaagaag
      361 tgtttagcaa agcgtgggct tcaactcagt cggcgccgaa tcggtcgaat catgaaacgc
      421 cgcggattga catctaccta tacgatcgct cattttaaag ggcaacgaac agcttgtaat
      481 gaagcgaaaa cagcgaatgt attagatcgg acctttacac aagaacagcc attggaagcc
      541 atcgttacgg atcttactta tgttcgcgtg gggaaaaagt ggcattatat ctgcttaata
      601 cttgatttgt ttaatcgaga aattattggt tattcctgtg gtgagaagaa agatgcctca
      661 ttggtaaaag aagcctttgg acggataccg tattctttaa cagac
//
LOCUS       DACENJ010000375          673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.457, whole genome shotgun sequence.
ACCESSION   DACENJ010000375 DACENJ010000000
VERSION     DACENJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.457"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            40..>673
                     /locus_tag="IW748_002724"
     CDS             40..>673
                     /locus_tag="IW748_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1105607.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKILEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKINNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNI"
BASE COUNT          271 a          103 c          111 g          188 t
ORIGIN      
        1 taaaatatca gctaaaataa aagttaagga gcattgagta tgacaattaa aactagtctc
       61 caaaatcaag cttaccaatc tattcgccga caaattatct atgctgactt agaacctgga
      121 aaaaaagtat cagaaaaaat attagaagaa acactttcta ttggtcgtac accaatacgt
      181 gaagcactga ttcaactccg acaacaaggt cttgttgata caattcctca aagcggaaat
      241 tatatctcat taattgattt agattcggcc aaaaatgctc gatttgtcag agaacatttg
      301 gaacgacaaa ttatgttaga gtgttgtgcc aaaattaata accaaaaaaa acggatttta
      361 gaaactataa tttcagaaca agaaaaagct gttagtcaaa aaaatgctcg tagttttttt
      421 cataatgata atttattcca taaagcttgt tttgaaattg cagagcgaga agaagtatgg
      481 caatggttag aaaataacaa tacacactta gaacgatttc gttggctacg agtaaaaacg
      541 gaaggattaa agtgggaaaa aattatggaa caacattatc aactatttga ggcattaaca
      601 aaacaagatc cagaagaagc taactttcta acatcgttgc atcttcattt aatgcttaat
      661 gagcaaaata ttg
//
LOCUS       DACENJ010000376          663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.460, whole genome shotgun sequence.
ACCESSION   DACENJ010000376 DACENJ010000000
VERSION     DACENJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.460"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>663
                     /locus_tag="IW748_002725"
     CDS             <1..>663
                     /locus_tag="IW748_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105608.1"
                     /translation="QEAKYTPEAMNREEMLRKRVEGRTGTNTDEKEALTKERIQEAKN
                     SNTGLEKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGT
                     KTIGVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVR
                     ENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDS
                     EIR"
BASE COUNT          280 a           91 c          150 g          142 t
ORIGIN      
        1 caagaagcta aatacacgcc tgaagcaatg aatcgtgagg aaatgttaag gaaacgtgta
       61 gaagggcgta caggaactaa tacggacgaa aaagaagcgt tgaccaaaga aagaatccaa
      121 gaagctaaaa actcaaatac cggattagaa aaattggtca aagaaaatct acaaaattct
      181 acaagtagtc aaggtggtcg atcagtagat gtccgagaaa atattcaagg ttcattgaat
      241 ggtcatgtcg gacggaatgt tgatgtaaga gagaatttac aaagttcttc aaatggtcaa
      301 ggtactaaga caattggtgt tcgagaaaat ctgcaaagta gcagtacaag tcaaccaaaa
      361 acagtagatg ttcgagaaaa tgtacaaaat tctacaagta gtcaagggtt taaatcagtg
      421 gatgttcgag agaatgtgca aggtagcagt gcaagtcaac caaaaacagt agatgtccga
      481 gaaaatgtac aaagttctac aagtagtcaa ggattcaaaa cagtagatgt tcgggagaac
      541 gttcaaaaag atagtggaga aatgaaagaa aaaacacaaa aaatcaatat cgttgaagac
      601 agaaagtcag cgaaaaaatt tgattcaaat catgaaacag tgattattga tagtgaaatc
      661 cgt
//
LOCUS       DACENJ010000377          644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.464, whole genome shotgun sequence.
ACCESSION   DACENJ010000377 DACENJ010000000
VERSION     DACENJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.464"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            13..>644
                     /locus_tag="IW748_002726"
     CDS             13..>644
                     /locus_tag="IW748_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1105609.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNL"
BASE COUNT          245 a           70 c          128 g          201 t
ORIGIN      
        1 aaggagaaag ttatgttaga agtgaaaaag cttaatgttt gttataacga ttttttagca
       61 ttgaacgaga tttctttaaa aatacaagaa ggtgctttaa caggtattat tggtccaaat
      121 ggtgctggaa aatctacatt gcttaaagca atgttaaata ttatccctca tcagggagag
      181 gtctccatta ataatgaaga tatcaataaa aaactggtga agattgcata tgtagagcaa
      241 aaagctgata ttgattttac ttttccaata aaagtaaaag aatgtgtatc tatgggaaca
      301 tatgccggaa tgaaagtttt tcaaagaatt aaaaatgcag aatggcaaag agtttcaaag
      361 gctttggaaa aggtcgatat gggaaaatat tctaatcatc aaataggaga attatctgga
      421 ggacaatttc aacgtgtact tttagcaaga tgtcttgctc aaaatgcgga tttcatattt
      481 ttagatgaac catttgtagg gatagactta gttagtgaac gaataattat ggatacgtta
      541 aaagaattga aacatcaggg aaaaacagtg ctaattgtgc atcatgattt aagcaaggta
      601 aagaagtatt ttgataatat tattcttttg aatcgaaatc taat
//
LOCUS       DACENJ010000378          632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.470, whole genome shotgun sequence.
ACCESSION   DACENJ010000378 DACENJ010000000
VERSION     DACENJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.470"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..632
                     /locus_tag="IW748_002727"
                     /pseudo
     CDS             1..632
                     /locus_tag="IW748_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          229 a          115 c          133 g          155 t
ORIGIN      
        1 taagtatact tttgaactta aattaaagat cgttcatgat tacttggatg gtaaaggcgg
       61 aagtgattat ctggcaaaga aatattccat taaggctcca tcacaagtga agcgctggat
      121 caatgcctat caagaatttg gtgaagaggg tctagtaaga aagcgacaaa agaaaaaata
      181 ttctgttcaa tttaagctgg atgcgataga gttatatcta acaagtgagc tgtcctatcg
      241 tgaagttgcc aacacactta acatgaacaa ccctaatttg atcgctagtt ggatgcgtca
      301 attccgtgag ggtgggatcg atggactctc aaaaacgaaa ggatgtccac ctatcttgtc
      361 taagaaaaat gaacccaaaa ataagaaaaa atctacaact aaagcaacgt ctaaagaacg
      421 tgaacgcatc gaggagttgg agaaacgagt ccgttccctt caaattgaaa atgccttttt
      481 aaaagaattg aggaagctgc gaaaacagga agcccaacaa cgacgaatga agcaatcgca
      541 cgaatcatcg caagcctccg aggaccgttt aaattagttg agcttctaga aactttaaag
      601 ttccctaagg caatctttat gtactggcaa aa
//
LOCUS       DACENJ010000379          620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.472, whole genome shotgun sequence.
ACCESSION   DACENJ010000379 DACENJ010000000
VERSION     DACENJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.472"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..597
                     /locus_tag="IW748_002728"
                     /pseudo
     CDS             1..597
                     /locus_tag="IW748_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          214 a          112 c          115 g          179 t
ORIGIN      
        1 agttatgcga ttgctccgac tgaatcgacc tacgtttgga atgagttgtt acaggacatt
       61 tactccagag gggttcatga tgtcttactg tttattaccg atggcttaaa aggcatgaaa
      121 gatacgattc accagattta tcctaaagct aaataccaac attgctgtgt tcacgtttcc
      181 cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag aaatctgtga tgattttaag
      241 gctgtttatc aagctagctc aaaggaagag gcaaatacct ttttagggag tatgattgag
      301 aagtagcaaa aaacttatcc taaagtgacg cagtcactga taaaaaatca agatttattg
      361 actttttatg agtttccgcc tggtatccgc cgaagcattt actcaactaa tttaatcgaa
      421 tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag agcagtttca aaatgaagaa
      481 tcactagagc gtttcctagt atccatcttt gatacataca atcaaaaatt cttaaataga
      541 agccataaag gcttccaaca agtgacggat acattagctt caatgtttac tgagtaaccc
      601 attattttgc agaaggacga
//
LOCUS       DACENJ010000380          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.492, whole genome shotgun sequence.
ACCESSION   DACENJ010000380 DACENJ010000000
VERSION     DACENJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.492"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..473
                     /locus_tag="IW748_002729"
     CDS             <1..473
                     /locus_tag="IW748_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105610.1"
                     /translation="KKGTELLRLETEKYIMNVYLERSELVENFVSYTICRKHYYSESE
                     YYSIFESMELMEEEMEELNSKDATIDDYYLVFTRFLTCGTESIVLMGMDYELAVPEAF
                     LREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT          206 a           79 c          111 g          130 t
ORIGIN      
        1 taaaaaaagg aacggagctt ctacgactag aaacagaaaa atacatcatg aatgtttacc
       61 tagaaaggag tgagctggtc gaaaactttg tcagctacac gatttgcagg aaacactatt
      121 actcagaatc agaatattat tcaatttttg agagcatgga attaatggaa gaagaaatgg
      181 aagagctaaa cagcaaagat gcgacgatag acgattatta ccttgtattt actagatttt
      241 tgacgtgtgg aacggaatct atcgtgttaa tgggaatgga ttatgaactc gctgtacccg
      301 aagcattttt gagggaatat gtagaagaag tagataaccc aggcgacgac tggtacacga
      361 ctttaactga ttttttagac acctatactt acgacgatac agaagaaatc caaaatgaac
      421 ttgaccgtaa acgaatgcca tatcaacgat ttgaaaggaa aaaaaggcaa taatcattta
      481 taccgaaaag tgagaatgtg tgaaataagg acaaaaaacc tagtaa
//
LOCUS       DACENJ010000381          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.denovo.505, whole genome shotgun sequence.
ACCESSION   DACENJ010000381 DACENJ010000000
VERSION     DACENJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.denovo.505"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>487)
                     /locus_tag="IW748_002730"
     CDS             complement(<1..>487)
                     /locus_tag="IW748_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ1105611.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACENJ010000382        10309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.guided.086, whole genome shotgun sequence.
ACCESSION   DACENJ010000382 DACENJ010000000
VERSION     DACENJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.guided.086"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..439
                     /locus_tag="IW748_002731"
     CDS             <1..439
                     /locus_tag="IW748_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105612.1"
                     /translation="AVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQFSQTLARFIP
                     TEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKRSPFNDNKTR
                     LYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            511..906
                     /locus_tag="IW748_002732"
     CDS             511..906
                     /locus_tag="IW748_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1105613.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            890..3343
                     /locus_tag="IW748_002733"
     CDS             890..3343
                     /locus_tag="IW748_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1105614.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            3340..5367
                     /locus_tag="IW748_002734"
     CDS             3340..5367
                     /locus_tag="IW748_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105615.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            5364..6386
                     /locus_tag="IW748_002735"
     CDS             5364..6386
                     /locus_tag="IW748_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1105616.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            6403..7332
                     /locus_tag="IW748_002736"
     CDS             6403..7332
                     /locus_tag="IW748_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1105617.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            7577..7693
                     /locus_tag="IW748_002737"
     CDS             7577..7693
                     /locus_tag="IW748_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1105618.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            7709..9628
                     /gene="tet(M)"
                     /locus_tag="IW748_002738"
     CDS             7709..9628
                     /gene="tet(M)"
                     /locus_tag="IW748_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1105619.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            9726..9914
                     /locus_tag="IW748_002739"
     CDS             9726..9914
                     /locus_tag="IW748_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1105620.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(9972..>10309)
                     /locus_tag="IW748_002740"
     CDS             complement(9972..>10309)
                     /locus_tag="IW748_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1105621.1"
                     /translation="KYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTNIENKGQ
                     HPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLMEALAKG
                     INEARNIEG"
BASE COUNT         3455 a         1747 c         2038 g         3069 t
ORIGIN      
        1 agctgtctat gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt
       61 tggtatcgaa tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc
      121 tcgatttatt ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg
      181 tttctcatta agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag
      241 aagaaatcct ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa
      301 aacgagattg tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt
      361 cagatattca atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac
      421 aaaaaataaa attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt
      481 tttctatttt tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt
      541 tggtcagtag aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc
      601 tttacgcaaa tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta
      661 ccaccacttt ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc
      721 actgggttca tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct
      781 atcattacct atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa
      841 aaagaaacaa tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc
      901 gattaaatat atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta
      961 tgaattaatt ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga
     1021 taatttctgt caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc
     1081 aaccgaatca agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt
     1141 aaaagagata gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg
     1201 tgacaatcaa gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga
     1261 agtatccgta aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg
     1321 tgtgaatcac aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt
     1381 ttctaagatg gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt
     1441 ggagaaagat gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt
     1501 tgatgactac gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa
     1561 atacgatatt ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat
     1621 tgaacaggaa gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga
     1681 attggatttc cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt
     1741 tgataccagt atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa
     1801 taaaaagaaa gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag
     1861 tactaatgtg gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac
     1921 caaagaatct atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga
     1981 gctgaaacaa cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt
     2041 acgacctttt ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata
     2101 tatcaatgac tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc
     2161 tacacaaatg attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag
     2221 aaatatttat ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc
     2281 attagcgtcc gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct
     2341 ggtctattat tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg
     2401 tggtgaatgg aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac
     2461 gagtgagaac gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga
     2521 ttcagaaagc cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag
     2581 cgagaagttt cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg
     2641 gttgttattg gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc
     2701 tgaccatatc gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag
     2761 tgtcacccaa tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt
     2821 cttacccgat tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc
     2881 aatgctgatt gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt
     2941 taaaattgtt gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct
     3001 atctatgcga ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca
     3061 aaacgcggac gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt
     3121 tcgttccact gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga
     3181 agatgaaaac aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga
     3241 catctatggt cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc
     3301 ctttgatacc agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa
     3361 tttcttcact atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc
     3421 acgctgggca atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat
     3481 gaatactcca agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg
     3541 ggctggcttc catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat
     3601 gccattacta attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg
     3661 gtgaaagaag cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac
     3721 atgcagacga ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt
     3781 ttcctccttc tttttattct cattgttgga atctatgttg cctatacagg attggttaag
     3841 cgagaaacga cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg
     3901 gcttccttta ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat
     3961 gtcagtaccg ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa
     4021 gggaaagaca gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca
     4081 tggctattgc ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa
     4141 agtttattgt ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt
     4201 gaagaaattg aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta
     4261 ggaacagtct tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta
     4321 acaggaatca tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc
     4381 agtttcctat taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag
     4441 ttatttaatg tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt
     4501 atttccacta tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta
     4561 caaattgtga cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact
     4621 ttacaaagca acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg
     4681 atgaatcgac aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca
     4741 gggtcaacga agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat
     4801 actcgtcaaa atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag
     4861 caacaaggcg gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa
     4921 aatcgtctag tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat
     4981 gtcaaatatg cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa
     5041 caatctatgg cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa
     5101 aaacgaaaga acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga
     5161 aaagaaaacc catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa
     5221 accgtcacgc ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa
     5281 aaagagcaaa aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga
     5341 acggataagc gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct
     5401 tctctctctt cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca
     5461 gtggcgaaaa caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag
     5521 tgttggcaca ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg
     5581 tttcttatat cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc
     5641 aaagttccga atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac
     5701 aaggggttaa atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag
     5761 attctgttat ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg
     5821 ggaaaaaata tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa
     5881 aagcagacta tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg
     5941 gaaatcagtt ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat
     6001 ttgatggtga aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct
     6061 atgtctttgg aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg
     6121 tgtatgacaa agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc
     6181 aagaaatctc tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata
     6241 atgcaggcac atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg
     6301 caggcgaccc aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg
     6361 gggctagacg tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag
     6421 cgaaaagaaa agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt
     6481 ccgatgatta aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt
     6541 ggtagtgtcg gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt
     6601 gaaacagaaa tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa
     6661 tcctttgcga aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat
     6721 gaaaaattga cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt
     6781 aaggatattc ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg
     6841 aatgagaaca cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac
     6901 aaggaaacta tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg
     6961 gttattatca aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa
     7021 cagctagaaa gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta
     7081 gaaacgttct tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt
     7141 aatcatgtgt tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc
     7201 tttaccagaa aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa
     7261 acaaaggcaa cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag
     7321 attgtaaaat aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt
     7381 tgataaaaaa ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact
     7441 atttaaaagg agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata
     7501 gcgggaacaa ataattagat gtcctttttt aggagggctt agttttttgt acccagttta
     7561 agaatacctt tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc
     7621 tatggttatg cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct
     7681 tttgggtttt tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca
     7741 tgttgatgca ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac
     7801 agaattagga agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca
     7861 gagaggaatt acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa
     7921 catcatagac acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt
     7981 tttagatggg gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat
     8041 attatttcat gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga
     8101 ccaaaatgga attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga
     8161 aattgtaatc aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga
     8221 atctgaacaa tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc
     8281 cggtaaatca ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg
     8341 ttctctgttc cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat
     8401 agaagttatt actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg
     8461 aaatgttttc aaaattgaat atacaaaaaa aagacaacgt cttgcatata tacgccttta
     8521 tagtggagta ctacatttac gagattcggt tagagtatca gaaaaagaaa aaataaaagt
     8581 tacagaaatg tatacttcaa taaatggtga attatgtaag attgatagag cttattctgg
     8641 agaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg gagatacaaa
     8701 actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga
     8761 accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag
     8821 tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt
     8881 agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat
     8941 agaactaaaa gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac
     9001 cattcacatc gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc
     9061 gcttccgttg ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca
     9121 atcatttcaa aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg
     9181 ttggaatgtg acggattgta aaatctgttt taagtacggt ttatactata gccctgttag
     9241 tactccagca gattttcgga tgcttactcc tattgtactg gagcaagcct ttagaaaagc
     9301 tggaacagaa ttgttagagc catatcttag ttttaaagtt tatgcaccac aggaatatct
     9361 ttcacgggca tataacgatg ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa
     9421 aaataatgag gtcattatta ttggagaaat tcctgctcga tgtattcaag attatcgcaa
     9481 tgatttaact ttttttacaa atgggcttag tgtttgttta gcagagctaa aaggatatca
     9541 ggttaccact ggcgaacctg tttgccagac ccgtcgtcta aatagtcgga tagataaagt
     9601 aagatatatg ttcaataaaa taacttagtg cgttttatgt tgttatataa atatggtttc
     9661 ttattaaata agatgaaata ttctttaata tagatttgaa ttaaagtgga aaggaggaga
     9721 ttgttattat aaactacaag tggatattgt gtcctatttg tggaaataaa acaagactac
     9781 gaatacgagt ggatactata cttaaaaatt tccctttata ctgccccaaa tgtaagaacg
     9841 aaactttaat taatgttcaa aaaatgaata taataacaat caaagagcca gacgccaaga
     9901 cgcagagccg ataatttgag aaatgaaact ctcatcttat cggctctttt tgtttatctg
     9961 aattttactg actagccttc aatatttctt gcttcattga taccttttgc tagtgcttcc
    10021 attaaggata gttctttgtc tgtaaagcta tccatgtatt tttctacctg taaccttcga
    10081 gtgcttttaa tcaaattatc tgtaggtaag aaaaattcat caatagatac atggagtaat
    10141 gatacaagat catacagcac ttgtgtactt gggtgttgtc ctttgttttc tatatttgtt
    10201 aaataacgtg ggtcaatttc aatcattgtt cccacttgtt cacgagtgag accccgtttc
    10261 attcgagcct ctttaatcgc taaaccaacg gctctaaaat cgtatttat
//
LOCUS       DACENJ010000383         9421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.guided.102, whole genome shotgun sequence.
ACCESSION   DACENJ010000383 DACENJ010000000
VERSION     DACENJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.guided.102"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1822
                     /locus_tag="IW748_002741"
     CDS             <1..1822
                     /locus_tag="IW748_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1105622.1"
                     /translation="ISFLVHEKDRIGLIGTNGTGKTSLLNIIAGIDSSDGDRQTVFYP
                     TDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRAYEEALIELGENGEDPDVQKRY
                     AKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKISTLSGGQKKRVSLAQVLIDEPDL
                     LLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRYFLDRVANRIFELSFGKLYEYK
                     GNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRAGVQARGTKQQARINRFEDLKE
                     NLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQTLLEHLDLLIQSRERLGITGK
                     NGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQENEEMAPDKRMIAYLQEAAEEA
                     KTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKRRLFLLKLLIQQPNVLLLDEPT
                     NDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVADKLLVFQGNGKQELYYGNMSSY
                     LLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEWETIEDEIAELEEKISLLQEEM
                     NHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEWVED"
     gene            1834..2781
                     /locus_tag="IW748_002742"
     CDS             1834..2781
                     /locus_tag="IW748_002742"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1105623.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2801..3328
                     /locus_tag="IW748_002743"
     CDS             2801..3328
                     /locus_tag="IW748_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1105624.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3391..4044)
                     /locus_tag="IW748_002744"
     CDS             complement(3391..4044)
                     /locus_tag="IW748_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1105625.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4176..5018
                     /locus_tag="IW748_002745"
     CDS             4176..5018
                     /locus_tag="IW748_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1105626.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5176..6072
                     /locus_tag="IW748_002746"
     CDS             5176..6072
                     /locus_tag="IW748_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1105627.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6075..6788
                     /locus_tag="IW748_002747"
     CDS             6075..6788
                     /locus_tag="IW748_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1105628.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            6808..7326
                     /gene="msrA"
                     /locus_tag="IW748_002748"
     CDS             6808..7326
                     /gene="msrA"
                     /locus_tag="IW748_002748"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1105629.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7323..7550
                     /locus_tag="IW748_002749"
     CDS             7323..7550
                     /locus_tag="IW748_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1105630.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            7702..8253
                     /gene="lepB"
                     /locus_tag="IW748_002750"
     CDS             7702..8253
                     /gene="lepB"
                     /locus_tag="IW748_002750"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1105631.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8336..8878)
                     /locus_tag="IW748_002751"
     CDS             complement(8336..8878)
                     /locus_tag="IW748_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1105632.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(9115..9369)
                     /locus_tag="IW748_002752"
     CDS             complement(9115..9369)
                     /locus_tag="IW748_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1105633.1"
                     /translation="MLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQI
                     AKENGGTFHEVDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         3371 a         1648 c         1870 g         2532 t
ORIGIN      
        1 gatttctttt ttggtccacg aaaaagaccg tatcgggttg attgggacga acgggacggg
       61 taaaacaagt ctcttaaata ttattgccgg tatcgatagc agcgacggtg atcggcaaac
      121 agtgttttat ccaactgatt accgtattgg ttatctttct caagcagctg aattttccga
      181 tgaattaact gttctccaag cagtctttca aggaaacagc cccttgatcc aaactgttcg
      241 agcatatgag gaagcattga tcgaactagg ggaaaacgga gaagaccctg atgtgcaaaa
      301 acgttatgct aaagcagaag aacaaatgaa taaagaagat gcttggacta cagataccaa
      361 cgcaaagatt attttgcaaa aattaggaat cagtgaatta gataaaaaaa tcagtacttt
      421 atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg ttgattgatg aacccgattt
      481 actattacta gacgaaccaa caaaccattt agattatgaa gcgatcgagt ggttggaaaa
      541 ctatttgaaa caataccgtg gcgcactatt gatggttacc cacgatcgtt attttctaga
      601 tagagtagca aaccgaatct tcgaactttc atttggaaaa ttatacgagt ataaaggaaa
      661 ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt gtagaagtag aacaagaaga
      721 aaagcgcaag cggctttata aacaggaatt agcttggatg agggctggcg tacaggcacg
      781 tggcaccaag cagcaagcac ggatcaaccg tttcgaagat ctaaaggaga acttgtacca
      841 agtaaaccaa gaagatgatt tagagctgaa cctagcgaca caacgtttag ggaaaaaagt
      901 actggaaatc aaagatggca gttaccgtat aaatgaccaa acactgctag aacatttaga
      961 tttattgatt caatctcggg aaagactagg tattactggt aaaaatggtg cgggaaaatc
     1021 gacattgctt aatattctag ctggcagaat tcccctggat agcggtacaa tgtctatcgg
     1081 tgaaacggtt catcttgcgt attatacaca agaaaatgaa gaaatggcac cagataaacg
     1141 gatgattgct tacttacaag aagctgctga agaagcaaaa acagcagatg gcagtcaaat
     1201 cggagtagca gaattacttg aacgattttt atttccaaga tttatgcatg ggacactgat
     1261 aggcaaactt tcaggcggag aaaaacgccg gttatttttg ttgaaactgc tgattcagca
     1321 acctaatgtt ttattattgg atgaaccgac caatgatttg gacatagcaa cattgacgat
     1381 tttggaagat tattttagaa gttttccagg tgctgtgatc actgtttccc atgaccgtta
     1441 tttcttggac aaggtagcag ataagttatt agtattccaa ggaaacggaa aacaagaact
     1501 ttattacgga aacatgtcca gttatttact gaagcaaaaa gagacccagc agcctgctga
     1561 aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa aagaagaaat tatcttacat
     1621 ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca gaacttgaag aaaaaatctc
     1681 attgctccaa gaagagatga atcatcaagg agataatttc actcgattac aagaattgca
     1741 aaacgacgta tctgaaacag aagcacaact tgaagaaaaa atggcacggt gggaatactt
     1801 gagtgaatgg gtagaagatt aggaggaaaa aggatggaac aagcgtatct tgacttagga
     1861 agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa caggcacagg cacattaagc
     1921 ttattcggat accaaatgcg gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa
     1981 cgagtacctt ttggattgat caaaagcgaa ttgctttggt ttttaaaagg agatacgaat
     2041 atccgctatc tattacaaaa taaaaaccat atttgggatg aatgggcatt tgaacgctac
     2101 atcaaaagtc cagactataa tggaccagat atgactgatt ttggccatag atgtttgaca
     2161 gatcctgaat tcaatgaaga atataaaaag cagagcaagc ttttttgcga gaagatccta
     2221 accgatgatt cctttgctga aacatacggc gatcttggac acatctacgg ctatcaatgg
     2281 cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca
     2341 atcaaaaaga ctcctgattc tagaagattg atcgtttctg catggaatcc ggaagatgtt
     2401 cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat tttatgttca agagggacgg
     2461 ctaagctgcc aattgtatca acgcagtggg gatgtgtttc taggcgttcc gttcaacatt
     2521 gcaagttatg cgttgctgac tcacctaatt gcacatgaaa caggtttagc ggttggcgaa
     2581 tttgttcaca cattaggaga tgctcatctt tatatcaatc atatcgaaca aatgaaagaa
     2641 caattgagcc gagaaatacg cgcatttccg actttagttt tgaatcagga aaagcagtca
     2701 gtatttgatt ttgagatgga agatatccaa ttagaaggat atgacccaca tccggcgatc
     2761 aaagctccta tcgctgttta gaatcagaaa ggatgagaaa atgttagctg caatttgggc
     2821 acaggatgaa aacggattga ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga
     2881 tctgaaattc ttcaaacaaa tgacagaagc aaatacacta gtgatgggac gaaaaacatt
     2941 cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg attgttttga cgagagattc
     3001 ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga
     3061 aaaagaaaca gacggtatct ttttcatcgc tggagggtca gctgtttatc aagaatttct
     3121 gccattttgt acgatattat accgtacagt catccatcat gtattcgatg gagatgctta
     3181 ttttccacct gttgactggt ctgattggtc actgatcaat actagtcaag gagaaatgga
     3241 tgaaaaaaat cgttatccgc atcaatttga aacgtatcag cgtaatgaga atcctgtaaa
     3301 aagcgggaca ggcgaattga cgatataaaa aagctatgga tagactagct gttatttaga
     3361 gctgtcccat ccatagcttt atttttttta ttaagtaaag aataaaacag agaagtacat
     3421 aaaacctgca cctaacataa caaacaaatg ccaaacaaca tgcatatagc ggatagaatt
     3481 caagctataa aagaatgcgc ctatcgtgta agaaagcccg cctgctacca tcaaaataac
     3541 accagtcatt ccaagagagt gataaagagg aacagcagcc acgacacaga gccaccccat
     3601 aaaaatatag atgatcgtag atacttttga tactctctcc cgtttatgca acgtcaacgc
     3661 tttatatata atgccaagaa tcgcaaacaa ccagattaat acaaataaag tccaacctag
     3721 ccatcctcgg atactcagca aacaaaaagg agtataactt cctgcaatca aaagaaaaat
     3781 cgaactatga tcgaatactt ggaacacctt ttttgctttt gtaaaaatca atgaatgaaa
     3841 caaagtagac gttaaaaata aaagaatcat catcgatcca tagatagcat aagagacaat
     3901 atggagggag gaatgcaagt gggcaccttt tactaaaaga accactagtc ccgcgatact
     3961 caatccagtc ccaattccat gggtgactgc attaaacact tcatttaata tttcatacgt
     4021 tcgattaaat ttcgtttttt ccatgaaaac ggctccttta ctaggtgtaa ttaatgaaaa
     4081 atcattattt gtctgttata ctaaaaatag aaattgtctt acatgtagtt taacatgaat
     4141 aaggcatctt gttaaagaaa gagagagaga aaataatgac aaatgtaaaa atcgtaacag
     4201 attcttcatg tacgatggaa aaaaatgtac gcgatgcttt aggcattcgt gtcatgccgc
     4261 tatccatcat gatcgacgga acagtatatc ccgatgatga ccaactagac ggcgaaagat
     4321 ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac aagccaacca ccaattggag
     4381 aattcatctc gatgtatgat gagctaggag aagacggcag tgaagtgatt tcgatccata
     4441 tgacaaaagg cttgagtgga acagtagaag cagcaagaca agccagtaat ctaacaaaaa
     4501 cgaaagtaac ggttatcgac agtgatacca ctgaccaagg attatctttt caggtaattc
     4561 gagcagctga attagcaaaa gaaggtaaaa cagtagagaa aatccttgaa gaagtcaaaa
     4621 aagtacaaga caatacaaaa ttatacatcg gaatatccac actagataac ttggtaaaag
     4681 gtggaagaat cagccgaaca actggtttgc tgtctagtgt tttgaacata cgcgtggtga
     4741 tgaatttcgc ccattctgaa ttaatccctg taactaaggg gcgaggaaag aaaacgttcg
     4801 ataaatggtt taataatttg aaaaatgaac tgaaagaatt gccaaatgtg cgacaaatcg
     4861 gtatttcaca tgcggatgca aaagaattgg cgcaagaatt caaagagggt ttgcaagaat
     4921 tattcccgga catgcatatc cctgtactgc atacgaatcc ggttatcgct acgcatactg
     4981 gtaaagacgc gtttgcaatc atgtattatg tagattagtc gaaaagagga taagccctca
     5041 gcctgaaagg cttgcagctt attctctttt tctttataaa attaggaaaa taattgtttt
     5101 cttttttcat aaagactacc tttttgataa aatttttgat aacattagaa ccaatcgaat
     5161 acaagaggtg aaaaaatgaa attaacgaaa cgacatttga tcctcgtttt gttcccatta
     5221 ttaattgcga tcatcatcta tgcaggttta gctgcttcta taccgaaagc tggcccacta
     5281 atgaaaccag taactactgt gagagaagat ccaaataaaa aagaaaaact tcactacata
     5341 gctatcggag attcgctgac tgaaggagta ggagatcaaa ccaagcaagg aggattcgtt
     5401 ccccttgtcg caaatgatct aaaagaccgt tataatttga ccgcggttga aacagaaaac
     5461 tatggtgtaa atggtgaacg aagtgaccag atcttaaagc gagtaaaaac aaaagaagaa
     5521 attcgaaaca atatcaaaac agctgatttt atcacactga ctgttggcgg taatgatttg
     5581 atgaaagtta tccaaaataa tttattcggg ttaacggtca attcctttaa aaagccactg
     5641 aaaaactatc aggaaaatgt cacagaacta ttggaagaat ttcgatccct caataaaacc
     5701 gcacctattt acattttagg catctacaat ccattttatt tgaactttcc tgagatcact
     5761 gacatgcaga caattgttaa caattggaac aaaggaacag aaacggtgac aaaaaaaatg
     5821 ccgaactgct actttatccc aatcaatgat ttgctttatc aaggattgaa tcaggaagta
     5881 ggtgtcacag aagaaggaac agatcaaaca actagcacga cagaaaatct aaatggcatc
     5941 aaaaataatg tgctctatga agaagatcat ttccatccaa ataatttagg ttatcaattg
     6001 atggcaaatg cagtgagaga tcagctgatc gagacacaaa accaatggtt agagaaggag
     6061 gatgatcaat aaacatgaaa acaagatctg acgtatcttc aaacaaaaca aagaaaacaa
     6121 attactggaa attcgccttc cttatccttc ttggggtagt acttggttcg accgtatttt
     6181 taggcagccg tatttttgct aatcgggaac cagaactccc agaaatacca gctcttacgg
     6241 aaagacaagg agatcctgtc ttaacgatca attcaaataa agaaaaagtc aatcaaatca
     6301 tcagcttttt ccttagtgag tatcaaaaag atagtgatat tgaatataaa ttttatttgg
     6361 aaaatgaagc actactcaat ggtactttcg aagtactagg ttttcctatc gatttctatt
     6421 tatattttga tccgtatgtg atggaaaacg gaaatgtcca actaaaagct agaagtcttt
     6481 ctatcggaac attgagtttg ccaatcaaag atgtgatgaa tatgatcaag agaaactata
     6541 aattaccaga ttggatcgaa gtcaatacag atgatctgac tatcatgctt aggttggatc
     6601 aattccgaat gcaaaacggg atgtatatta aagcagataa aatcgactta gtaaatgatg
     6661 acattcggtt tagtctatat ttgccaaaag atacctctga tgcttcaaac aactcaaata
     6721 ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa agaaaataaa aaatcatcgg
     6781 atgagtagta ttagaggggg aatcataatg gaacgagcaa tatttgcagg aggctgcttc
     6841 tggtgtatga tccagccttt cgacacgctt cctggtatcc atacagtgat gtcaggatat
     6901 actggagggc atgtaccaga tccgacttat gaacaagtaa aaagccaaac gactggacat
     6961 acagaagcag tggagattat ctatgatcca acattgattt catatgaaca attagtagaa
     7021 ctttactggc agcaaactga tccaactgac gctttcggac aattcgagga tcgcggtgac
     7081 aattatcgcc cggtgatctt ttatttgaca gaagaacagc ggatcattgc tgaaaaaagt
     7141 aagaaacagt tagctgaaag tggacgtttc gaacagccaa tcgttacaaa aatcgaacca
     7201 gcccaaccat tttatctagc agaagaagac catcaagagt attataaaaa gcatcctgaa
     7261 aattttaaaa gaaaccatgc acgccgggct gcctttatcg aagaaaattg gaggaacgaa
     7321 gggtgaatcg aagctttttt cattatttaa tgacactgcg aggcggtggc acacatgatg
     7381 ctttatctgt tttcgctaca gaagccggta aagacatcca atttcctaag cacagctcta
     7441 gctacgaaga aatttctaat tatctggaac tgaatgtcga ctatttgccg tccatggata
     7501 tctttgacga agcatgggaa aaatatttag aaaataatca tgacatataa gagatagttt
     7561 tgtactttat gatcaaaaaa gttttttgtc caatagccta attgaaagaa ttatttttat
     7621 ttcagctatc tagtcgcatt tttttgatat ttttcttata ataaagaaag aatgaaaaaa
     7681 agagggatgg attgatagag attgacaaaa aaacaacgct atattaatcg cttctggctt
     7741 gtattcaaat acttacttgt ttctgtattc cttgcgttca tgttgcgggg atttttattc
     7801 atcccggtac cagtggaagg caattcaatg gaaaacgtgt tgaagcaagg tgatatggtt
     7861 gtgatggaaa aattttcgga gatcagacga tttgatattg ttgtttttca attagcagat
     7921 ggcacgattt atatcaaacg agtaatcggt cttccaggag aaaatgtcag ctaccaaaat
     7981 gatcaattga agatcaatgg gaaagtagtt aaggaacctt atctaactaa aaacctgaaa
     8041 tcagatcatg caaatgcatc ctatacgacg gactttacac tacaagagct aacgggacaa
     8101 tccaagcttc cggaagataa ttattttgta cttggagata atcgaagagt ttcaaaagat
     8161 agtcgttcgt ttggcacgat caataaaact gatattctag gtaaagcaag atttgtctat
     8221 tatccacttg atgaaataaa atggatccag tgagaaacaa taaagcgtgc agtctgttga
     8281 gactgcacgc tttattgttt aatccaaaat cagtttacta ccttagtacg taaaatcaaa
     8341 cagctcgttt aaacactttt ctagcctcta aagttttgat aaatgaataa acaaccatta
     8401 tttgaatagg gatttggatc atattcctga tgaatctacc tagaaaaatt actttgaaat
     8461 ctgcaccaaa caaaacagac atcagccaag aattgataaa aaggctgact gggatcataa
     8521 tcaataattg ggcagcgatg acggaaggca ggtttatctt tttatgatat aaaaataagc
     8581 cattaataaa tccaccaagg aaagccgtga taagaaaagc agcttgaaac gttcctgaac
     8641 caaatatcgt gaaatttaaa gtgttcgaca acatttcaag caaaccagcc cagatcggtc
     8701 catatagata acccataagc gccatactta aaaatccgaa tgtgatttga aaataagcag
     8761 gaccaatcgc tatcttgtat aagatgacat caagggctaa taaaagagca gaggtcgaca
     8821 acaatcttgc attcatttcg ggcaatctaa aaacgtcttt tgcaatcaaa gtgttcaaaa
     8881 tattctctcc tttgtcagtt aataataaaa ggataccata aaatcaccaa tgtgtcatga
     8941 cacattggtg atttacatac actggcatct aacaattaaa aaaagggtga gaagactgct
     9001 tttataaact gtaaaaaaga gattgtacga ataaaaaaaa tagaggcgga gaatacttat
     9061 cgtatcatcc gcctttgttt ttttgttata aaaaacgtca gattagttat ctgcttacat
     9121 cttcatccat acgggttcta atgtatttcc gtctagatct tggacttcta atgaaaacat
     9181 ttcttcttca ggcattccca tatctacttc gtggaaagta ccaccatttt cttttgcaat
     9241 ttgagcaaat ttttttactt catccgcatt ttcaacacta aacgaaatca aagcaccact
     9301 tgttttttta ttatcagcta tttctttatc ttttataaat tttttataaa aatcatgaga
     9361 gagaagcatt atccaaaaag tgtcatccca taccattgaa ccagcttctt cagtagaaaa
     9421 t
//
LOCUS       DACENJ010000384         9233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.guided.105, whole genome shotgun sequence.
ACCESSION   DACENJ010000384 DACENJ010000000
VERSION     DACENJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.guided.105"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1432
                     /gene="lsa"
                     /locus_tag="IW748_002753"
     CDS             <1..1432
                     /gene="lsa"
                     /locus_tag="IW748_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1105634.1"
                     /translation="NLNFDTQWKLGLIGRNGRGKTTLLNILQNKLPYQGQVIHQQEFA
                     YFPQQTKDKERLTYYVLNDITDFEIWEIERELQLMQTDPEILWREFSTLSGGEKTKVL
                     LALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLKKKKQGFIVVSHDRGFIDEVVDHVL
                     AIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEKLKKEVSRLKKTAAEKAEWSRSREG
                     DKTKKQVGFIDTESRRVNKGAVGADAARTMKRSKAIVNRMETQISEKEKLLKDIEYID
                     SLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHFTIEPHQRVAISGPNGAGKSSIIHY
                     LLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGTLAEFAEKNQVDYQAFLNNLRKLGM
                     ERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYTWDEPLNYLDVFNQEQLEQLILNVK
                     PAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1499..2026
                     /locus_tag="IW748_002754"
     CDS             1499..2026
                     /locus_tag="IW748_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1105635.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2237..3298
                     /locus_tag="IW748_002755"
     CDS             2237..3298
                     /locus_tag="IW748_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1105636.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3301..4197
                     /gene="murQ"
                     /locus_tag="IW748_002756"
     CDS             3301..4197
                     /gene="murQ"
                     /locus_tag="IW748_002756"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1105637.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4211..6193
                     /locus_tag="IW748_002757"
     CDS             4211..6193
                     /locus_tag="IW748_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1105638.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6258..6704)
                     /gene="nrdI"
                     /locus_tag="IW748_002758"
     CDS             complement(6258..6704)
                     /gene="nrdI"
                     /locus_tag="IW748_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1105639.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            6915..8339
                     /locus_tag="IW748_002759"
     CDS             6915..8339
                     /locus_tag="IW748_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1105640.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(8520..>9233)
                     /locus_tag="IW748_002760"
     CDS             complement(8520..>9233)
                     /locus_tag="IW748_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1105641.1"
                     /translation="LSVLLVVLLIGTALLAKKYTTIIDPVEMTAQLFSATSWIVFIMI
                     AAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLVIYSVYLYLLAIIVTVLMKLI
                     LFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSLVLMIACFINSSGASISAGIV
                     FYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGNEEIMKQLTHLSTNQMLFGNL
                     AYIVLFLALGYLVFKRKNV"
BASE COUNT         2984 a         1613 c         2043 g         2593 t
ORIGIN      
        1 aaatctaaat tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa
       61 gacaacttta ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca
      121 gcaagaattt gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt
      181 gttaaatgat attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca
      241 aacagatcct gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa
      301 agtcctactg gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac
      361 gaatcatttg gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca
      421 aggcttcatc gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt
      481 agcaatcgaa aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca
      541 gaaaaaactt cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag
      601 taggctaaag aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa
      661 aacaaagaaa caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt
      721 gggtgctgat gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac
      781 ccagatcagc gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat
      841 gaatagccaa gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta
      901 tgaaaatctg ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat
      961 ttcaggtcct aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa
     1021 cggcaaggtt ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca
     1081 aaattatgaa gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta
     1141 ccaagcattt ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa
     1201 gatcgagcag atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca
     1261 gccagctgaa ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga
     1321 acaattagaa caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca
     1381 aaccttcctg gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta
     1441 atctcaagaa caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat
     1501 gctttatgta acgagacacg gagaaacaac ttggaacgca caaggattag tttgcggacg
     1561 ggcagatgtg ccgctgactg aaaaaggaca gatgcaggca cagaaactag cagaaaaggt
     1621 agtagatttg cctgttccaa tcacgaaaat catccattcc cctcttcaac gagcaagaga
     1681 tacagcacaa gcagttgctg atcgtctcag cttgccgctg accgttgatg aacgattggt
     1741 agaaatggat tttggggatt atgatggact gccaagcaaa gatgagaact ttcaaaaagc
     1801 acgtttagcg ttcgctgtac gattcccaaa tggggagtca gtcttagatg tgtatgcacg
     1861 gatcgttcct ttattgaaag aatgcatcga agacgaagaa aatgtctatc tgttagtttg
     1921 ccataatgcc ttgataagag tgatcaatgc ttattttcat ccgatgccta atgaggggtt
     1981 ctttacattc atggtagata atacagaatt ggtttcttat gagtaattat gtttttgtgt
     2041 tatttaaata aaatatttca aggcactgct tttgcttagc agtgcctttt ttaggttatg
     2101 acaattcttt tgataatata aactcaacct ttagaaatga aatttaatta ttttaataaa
     2161 agtattgaaa ttttgtttct ttagatatat actctagtca tagaaatgaa gacggataca
     2221 gaaaaagagg gataatatgt ttggcttttc aatatttatg aacgattctt tgtcagaaga
     2281 gaagaaacag tatatccgcc aaatggcatc caatggcttt gtcggtattt tcacttccat
     2341 gcatatacca gaagatgatg tttcggcata tcaaaagcga ttgatcgaac taggaagcga
     2401 agcgaaaaag aataaactag aattgatggt cgatatttca ggagaggcat tggaacgagc
     2461 gggtttttca ttagaagatc taacgccttt gaaagcaatt ggtgtaacag gtttaagaat
     2521 ggattatcat atttccaatc agcagattgc cgactggtca catcaattga aaatcagttt
     2581 gaatgctagc acgatcacgc ctaaagatat cgatgaattg aaagaagcag aagctgattt
     2641 ctcacagatt gaagcttggc ataattatta tccacgtccc gaaacagggc tggacaaaga
     2701 atggtatcag aaaaaaaatc aatggctcaa aattcaaggt ctccttgtcc aaggttttgt
     2761 accaggagat acagagttga gaggcccact atatcaagga ttgccaactt tggaagaaca
     2821 tcgagggatc catcctcttg caggcgctct agatctatta gcaagtaaca cggatattgt
     2881 ttacatcgga gatgcaggtc tgtcagaaaa cgttcaggag caatttgctt ctttccaaaa
     2941 agaacagaca gtcttgctcc atacggaacc tgtagatgaa gaattttacg aatatatatt
     3001 agggaagcat acgaatcggc aagacgatgc aagagatgtc atacgaagcg tagatgcacg
     3061 ttttcgagag attccgccta tcccagcaag aaatacagct acgcgtatga aagggtctat
     3121 tactttagat aacgaaaagt atttgcgcta tatgggtgag atccaattaa ccaaatatga
     3181 tttgccagcg gatgaaaaag tgaatgtagt agctaaagtt atcaaagaag agctaccatt
     3241 gatcaaccaa atcaaagctg gtatgaacta tcaatttata agaaaagaag gaagataaaa
     3301 atgagcaaaa tcaatttaga gaacctaaca acggaacgca gaaaccaaga aacttttgga
     3361 ttagatgaga tgagtatttc tcaagccgtt caactaatga acaaagaaga tcagaaagtg
     3421 gccgctgcgg ttgaaaaaga actgacaagt atcgaaccag tgattgctaa cacgattgag
     3481 tcctttaaaa aaggtggacg tctgatttat atgggagccg gaacaagtgg aagactaggt
     3541 gtgttagatg ctgctgaatg cgtaccgaca ttcggtgtag aaccggaaat ggtcataggt
     3601 ctaattgctg gtggagaaga agcaatgact gtagctgtcg aaggggccga agattcttat
     3661 gagctagggc aagaagatct aatcaagctg aatttgacaa aaaatgatat agttattggt
     3721 atcgcagcta gcggtcgtac accatatgtg atcggtggat tagattacgc aaaatcgatc
     3781 ggtgctgtca caggaacgat ttcttgcaat aaacaagccg aaatcagtaa acacgcggat
     3841 ttcccaatcg aagtcgatgc aggtccagaa tttttgacag gctctactcg attgaagtca
     3901 ggaacagcac aaaaacttat cttgaatatg atttctacta tcagtatgat tggtataggt
     3961 aaagtattca ataacttgat ggtcgatgtc aaaccaacaa atgaaaaact aatcgaacgc
     4021 tcaaaacgaa tcatcatgca agcgacagaa gctgattacg aaacagctga aaagtacttt
     4081 gaagaagcag atcaaaatgt taagctggct atcgtcatga ttttgacaaa tagtgataaa
     4141 gaggaagctc aagcaaaatt agctgaagca aacggatttg taaaagggac cttatgaggg
     4201 ggaaaagacc atggcaaaaa acgaactgac tttggaagaa ctggcaaaac aaatttataa
     4261 aggggcaggc ggcatgggca atgtagaaag cattgtccac tgcatgactc gtgtgcgtat
     4321 gaagatcatc aatgatgatc ttgtaaatgt ctctgaatta aaagcgattc caggagtgct
     4381 tggtgtagta gaagatgaac agcttcaagt catcatcgga cctgggaaag tgaacaaagt
     4441 agcacagcaa atggttgatt tggcaggcgt gaaattagga gaaaaacttt cttctgtaag
     4501 tacagctaca gcaaattcat cagcatctgg aaaagaccgt gtaaacgaac gggcacaaga
     4561 aatgaaagcg gcacaaaaat cgaaacaaaa accatctaaa atcaaatcgg tattaaaaga
     4621 catttctaat atctttgtgc caatgattcc tgcatttgtc ggtactggga tcgtggcagg
     4681 gattgccgca gttttagcaa acttagtcac agccggaaca cttgatgcag caacatggca
     4741 acaatatatc gatgtgatga acatcttgaa aaacgggatg tttagctacc tagttatcta
     4801 tacaggtatc aatgctgcac aagtttttgg tgcaacacca actcttggtg gagttatcgg
     4861 ggccgttgtg atgctgacag ggatgaatcc ggaagcacca ctgaaaaact tatttactgg
     4921 tgaagctctt gcagctggac aaggtgggat cctaggtgtc atcttcgctg tttggctatt
     4981 gtcaatcgtt gagaaaaaac tgcataaagt agtacctgat gcggtagata tcatcgttac
     5041 accaactctt tcgttgattg ccatcggtct gattgaaatt ttcttcatca tgcctttagc
     5101 aggtttcatt tctgatggca tggtcggtgg gatcaactgg gtacttggcg ttggtggagc
     5161 atttgccggc ttcgttctag gcacattatt cttgccgatg gttatgttcg gactgcatca
     5221 aattttgact ccaatccatg tgcaaatgat tgatgaaact ggtcgtacgc tattgcttcc
     5281 aattcttgcg atggctggtg caggacaagt gggtgcagca ttggcattat ggattcgctg
     5341 caaaaaagat aaagaattaa cagaaatgat caaaggagct ttaccagtcg ggatcctagg
     5401 aatcggcgaa ccattgattt acggagtaac attacctcta ggacgcccat ttattactgc
     5461 ttgtatcggt ggaggtatcg gcggggcagt gatcggaatg atcggaaatg tcggggctat
     5521 cgctattgga ccttcaggag ccgctttgat cccattgatc tctgacggta aatggttagg
     5581 ctacatcctt ggattattgg cagcttatgc tggtggcttc gttgctacat tcttcttcgg
     5641 tattcctaaa gaccgtttag aaaaagaaga attagcgact gaaacagcca cagtaaatga
     5701 agtgcctgaa aaagtagtac ctcaaacttc agctgaaagt gtgactatcc tctcatcagt
     5761 tgctgacgga acagttgttt ctttagataa agcaagtgat ccagtttttg cacaaaagat
     5821 gatgggtgaa ggctactttg tagaaccaga aaacggacat atctattctc cagttactgg
     5881 ggtagtcagc tctgtcttcc caacgaaaca cgctattggg atcacaacgc caaatggttt
     5941 agaaatcttg cttcatatgg gaatcaacac agttgactta ggcggtacgc catttgatgt
     6001 gaaagtaaca gaagggcagc aagtgacacc tgatacgctt gtagcagaag ctgacttaga
     6061 agcgatcaaa gcagcaggca aagaaacatc catgatcgtc ttggtaacca atatggatcg
     6121 tgtaaaaaac tttgtcttgg aaaaaacagg taaagcaaaa gcaaaagcac ctgtgatgga
     6181 tgtcaaagct taatatgata gaaacaatag aaacgaaaga agaaactgaa atctattttt
     6241 cagcttcttc ttttttatta tttattgttt tctgctaaaa tctgataaat tcgtgtcaca
     6301 tcagatggtg ttccccgtaa ttcataatct gccaagaaag gaaagccaaa tgtttgtgca
     6361 tattgtttgg ctgttagaca atactgatta ttgaagtttt tatttccgct accgactaca
     6421 ccaagacaaa gtttatgatt gtcttcatgt tccagatatt cacgcatcgt ttcggtcaag
     6481 atttctgtat caccgttatc taaaccattt ccgccgtcta aataagtagg aacgaaagta
     6541 aaaaatggtt ctgtttcttt cgtaaaatca ctattttcat ggatttcttt taaagtaact
     6601 tcaggattga caggatcttc tgcatgcatt ttttcagcat attctgctag atgtttggca
     6661 aaggcacgcg tatttcctga aatcgatata tataaaatgt tcatggatca tcctccaatt
     6721 ctttcctttt agtatagcat cttttttaag ttttttggtg gtgtaatttg gaaagggact
     6781 aggcgctttg cttgtataat gagaaaaaag ccggtagaat tatatttcta ttaaagtttt
     6841 tattaattaa gtaaaaataa aaaaggtttg ttatactttt tgataaaagc ggttttattt
     6901 tggaggatgc ttacatggat acagagcaac agaaaaaagg aattggttta attccacttg
     6961 ctgccttagt aattggttca gcgattggag gtggcgtatt tggtattatg acggacatct
     7021 ccggatcggc tggcggccca gctattttca gttgggtact cgtaggaaca gcaatgttga
     7081 tgctctcctt gtcgatcaat aatatcaata aaaaacgtcc agatttaaaa ggcgggatct
     7141 ttagttacgc agaagaaggt tttggccgtt tcgctggttt tatcagcggt tggggttact
     7201 ggctcacatg ctggttaggc aacgtagctt ttgcaacatt gttgatgagc gcattaggat
     7261 atttcttccc agtatttgaa ggtgggcaaa atctggcatc gatcgtgatg agtacagtct
     7321 ttttatgggg atatgcatgg ctagtgaatc ggggagtcga aaatgctagt ttcgtcaatg
     7381 ctatcgtcac attctgtaaa ttagtgccat tatttttatt tattgtagta gctatcagca
     7441 cgttttcgat ccaacaattc attgataact tttctagcga cattatattg aatgcgaatg
     7501 gacagccgat cccgttttcc agtcaagtat tgagttgcat catggtgatg ctttgggtat
     7561 ttgttggtat tgaaggagcc tctgtcgtag gttcacgagc aaaaaaacaa tcagatgttg
     7621 ggaaagcaac gattctaggg atattcggct tgattattat ttacgtcttg gtttccatgc
     7681 ttccatatgg gacaatgacg atgaatcagc tgcaaacgat caaggcgcag cctgctatgg
     7741 gctatgtgtt tgaaatgatg gttggtaaat ggggagcagt cgtgatcaac ggcggtttga
     7801 tcatttcttt agttggtgtt tggctctcat ggacgatctt accgattgaa acaatgggaa
     7861 acatggctga agacggactt ttgcctaaag catggggaaa actcaacaaa aatggggcac
     7921 caacttatgc tatcgtattg acgacgttat gtacgaatgt ctttttactt tcattattat
     7981 tcactgacag tgcttataac tttgcttaca cacttggtac agcagctatt ttcttcacgt
     8041 ggcttttctt aggattgtac caaatgaaac tttcttatca gcggaaagaa tgggtccaat
     8101 ttttagtagg gcttttagca agtgcctttc aagtatgggc tatgctagtc gtagccttta
     8161 atgaagtaat gcttgttctt gttcttttct tgccaggcat ttatttttac ctcaaagcac
     8221 gtaaggagca aaacgagcct gtacaacata tccacttaga taagatttta cttataggtg
     8281 tgacgatggt cggagtgatt gcaatggttt tatttgccac tgggatgctt cacgtataga
     8341 agtataaaag aagtataaag agaggttgtg agcctgtcgt ttattcaaaa acatactagg
     8401 aaaggagctg tgacacaaat gttgtcacag ctcctttttc agaataaacg gtgtttaaga
     8461 gacagccaat cggcaataag gctgaacgtc tctttcacag cctctttttt gatgagtcat
     8521 catacatttt ttcgcttaaa tacgagatat cctaatgcta agaacaaaac gatgtaagct
     8581 aaattaccga atagcatttg attagtagaa agatgagtca attgcttcat gatctcttcg
     8641 tttccaattt ggttttgcag attcaacata ctgatcggat tccatttgat ccactcccat
     8701 ttttggatga gtgcaataag gataccagat atgattgacg aagcaaaata aaagacgatt
     8761 cctgctgaga tggatgcgcc ggaactattg ataaagcaag caatcatcaa cactaaactt
     8821 aaaatcagcc ataaaccgat ataactaccc aaagtataag tgaacaagct ttggatcagt
     8881 gtttgaccag tagaaacctt ctccgtgaag gaaatcgaag ggaaaagaat cagtttcatc
     8941 aacacagtga caataatagc taaaaggtat aaataaacag aataaataac taatgtgatc
     9001 catttgctta ctaatatttc tcctcgagaa tactttcgat aaaggagatt tttcaatgta
     9061 ccatattgtg cttccatcga aatgatcgtg cttgcagctg caatcatgat aaagacaatc
     9121 caagatgtgg cactaaaaag ctgagcagtc atttcaactg gatcaatgat cgtcgtgtat
     9181 tttttggcta gaagtgctgt tcctatgagc aaaacaacta atagaacaga taa
//
LOCUS       DACENJ010000385         7595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.guided.127, whole genome shotgun sequence.
ACCESSION   DACENJ010000385 DACENJ010000000
VERSION     DACENJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.guided.127"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            209..850
                     /locus_tag="IW748_002761"
     CDS             209..850
                     /locus_tag="IW748_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ1105642.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLNFRNALLGSDIGNSSLESIEIEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYELTIV"
     gene            969..2054
                     /locus_tag="IW748_002762"
     CDS             969..2054
                     /locus_tag="IW748_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1105643.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSKEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            2051..3943
                     /locus_tag="IW748_002763"
     CDS             2051..3943
                     /locus_tag="IW748_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1105644.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            3950..4327
                     /locus_tag="IW748_002764"
     CDS             3950..4327
                     /locus_tag="IW748_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1105645.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            4478..5254
                     /gene="ant(9)"
                     /locus_tag="IW748_002765"
     CDS             4478..5254
                     /gene="ant(9)"
                     /locus_tag="IW748_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)"
                     /protein_id="HAQ1105646.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETS"
     gene            complement(5327..6874)
                     /locus_tag="IW748_002766"
     CDS             complement(5327..6874)
                     /locus_tag="IW748_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1105647.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(6976..7329)
                     /gene="tnpB"
                     /locus_tag="IW748_002767"
     CDS             complement(6976..7329)
                     /gene="tnpB"
                     /locus_tag="IW748_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1105648.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(7319..7513)
                     /locus_tag="IW748_002768"
     CDS             complement(7319..7513)
                     /locus_tag="IW748_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105649.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT         2670 a         1186 c         1463 g         2276 t
ORIGIN      
        1 cacttgtatt aatccggcag ataagaacgc attctaatca ctcccgacaa ggcaagacag
       61 gcgaaaaaga caaataaatg aacgaaagga agaaacataa aatgcaaaac caaatagcga
      121 tgaagaaaaa accacctccc tctaaaacaa tccaaacaaa aaagaatttt gaaaaaccaa
      181 aaatcaaaaa cagaaagaag cgttcaacat gagagataga attttagaag ttaatcaaga
      241 aatggtaaac aagttaacaa atatcagtgg gaactatgaa aagtacgatg gtactttact
      301 tgataactat atttttcacg atacacaaaa tatcagaatt ggcagaagta aaccgagagc
      361 gttcattctc atcaaagaga aatatttgaa tgaatggaat agcacctatg aaatggttat
      421 gactgataac tttgaaaagg tattgaattt cagaaatgct ttgttgggtt ctgatattgg
      481 aaatagcagt cttgaatcta ttgaaattga aaataagacc gagaatgaag gtgaaggatt
      541 gattaagaat gtcttgaaaa tcgaacaaaa atatatcagt gaagatagaa gtttttatag
      601 agtcacaagc tcggttggtc tagcaaactt tgctactaac ttaattggaa acaatgctag
      661 tgaatctttg ctagtgatgt gtctgaatac aaagaatgag gtcattgctt atagcgaagt
      721 tttcaaaggt ggaattaatc aatcagtagc gataccaaga gagatttttc aactagcttt
      781 gctaaacaat tcagcaaaaa tcgcagtatc acataatcat ccgagcggat atgaattaac
      841 catagtataa taaataatag tagttaaagt agtttaagag gagatattaa agggatattt
      901 tactttaaat aaaaattaac aaggtttata atttgtagta gttaaagtat ttaaagaggt
      961 gggaacatgt gaaggttcaa aggatagaag tggagaataa gccgtatcca ttgtatttat
     1021 tactagataa agaataccag ctaatagaac cagtaatgaa atttattaaa tacttagata
     1081 atactggtaa gtctcctaat accattaagg catactgcta tcatttaaag ttgctgtacg
     1141 agttcatgga acagagaggt gttattctta atgatattaa ctttgagttg ttagcagact
     1201 tcgtaggttg gttgagatat ccttcagcat caaatgtaat tgatcttcag tcaaaaaaag
     1261 ccataagaga agaaacgaca gtgaatacaa ttttaaatgt agttatgagt tttcttgatt
     1321 atttaagtag attaggagaa tttaaatcaa ttgatgtatt taaacaagca aagggaagaa
     1381 atttcaaagg atttttacat catgttaata agggtagata ccaaaagaat gtcttaaagt
     1441 taagggttaa aaagaaacag ataagaacat tgagatcaaa ggaagttaag caaattattg
     1501 atgcttgtca tacgaaaaga gataaattaa ttttaatgct tatgtatgag ggtggtttaa
     1561 gaatcggtga agtgttatcg cttaggcttg aagatattgt cacttgggac aatcaaatcc
     1621 atttaacacc tagagatgtt aatgttaatg aagcttatat taaattaagg aaggaaagaa
     1681 caatacatgt gagtaaagaa cttatgtcac tttatacaga ttacttgata tatgagtata
     1741 gtgaggaatt ggagcatgat tatgttttta tttccttaaa agaaggctat tttgggaaac
     1801 cactaaagta ccaaagtgtt cttgatctag ttagaagaat agttaaaagg actggaatag
     1861 aatttacatc acatatgctt cgccacactc acgcaacgca gctaattagg gaaggatggg
     1921 atgttgcgtt cgttcaaaag agattaggtc acgcacatgt tcagacaacg ttaaatacct
     1981 atgttcatct ttcagatcag gatatgaaaa atgagtttaa taaatacctc gagagaaagg
     2041 agcataagaa atgaatgctt ctagtaaaag gaaaattatt agtcagagtg agattagcaa
     2101 aaaaatagct gtaatgaatg aagaaatgca ggggttttgg gctaataata gttgggatat
     2161 aagaaaatgt ccacatcctt ctgccataga attaagtaag aatcctgctt taaggaatcg
     2221 ttgggttcgt tttgaacgtg ttaaaaatct gtggttaaga acagaattga aatattttta
     2281 tttttaccat ttaaacaatg gaatatggaa tgcaaaaact gtctggatta gaaaaggaac
     2341 agtaattaat aaaatgttag atttcttaga tttaaagtat cctagcatta cttcaattac
     2401 tgaagttcct attgaaaaag caatgacgga gtatagaact tatttgacaa aacggggtgt
     2461 tagaattacc actactaatt ataagattac tgctaatcaa gaaaaaacac ctgtaaaagc
     2521 taattcctac tatgttacta atctaaaaca atttatggag ttttatgaga acttttattt
     2581 tgatggagag gagtgggata aagacgtttg ggatagacgt aacttacctt tgccagatga
     2641 taaggttaac ccaacacaat atgaatatac aattaacttt aaagggtttc ggaatacata
     2701 ttttaaacaa cttgtaaaaa gatattgtaa gttgagattg aacgtggata gcttttccta
     2761 tgtaagtgat attgcccaaa gacttaaaga gttctttaat tttctggaca tgaaatttaa
     2821 acaagttcag agagtacacc aattaacgag agtggaaatt gaagcatatt taagtgaact
     2881 aaacatgatg ggaataaaac ctagtacaat aactgggagg atctctatat tggaaggact
     2941 atttagtacc cttcttaggc tagaatggga tgatgttcct tccaaaatat taatttattc
     3001 tgaggactat ccgaaaatac caagagcaaa accacgcttt atagatgaat tcgtcctaga
     3061 gcaattgaac agtcatcttg ataaattacc cgaatatata gctacgatga ctatgattgt
     3121 tcaagaatgt ggaatgagga taagtgaatt gtgcaccttg aaaaaaggct gtctattaga
     3181 ggacaaagat ggagatttct ttttaaagta ttatcaatgg aaaatgaaaa aggagcatat
     3241 agttccaata tctaaagagg tagctttact tattaaagtt cgggaagata aagtttcaga
     3301 ggaatttcca gatagtgaat acctctttcc aagaaaagat ggatcgccat taaaacaaga
     3361 aacatttaga ggtgagttaa ataaattagc ttatgagcaa aatatagtgg ataaatcagg
     3421 tgagatttat agattccatg cccatgcctt tcgccataca gtaggaacaa gaatgattaa
     3481 caacgggatg ccccagcata ttgtgcagaa atttttgggg catgaaagcc cagaaatgac
     3541 aagcagatac gctcatatct ttgatgaaac tctaaaaaat gaatttacta aatttcagga
     3601 aaaactggtt accaataatg gagatgtgct tgatctagat gaagataatg aagtcgatga
     3661 tgtagagctt caatggttca agaaaaatat aaatgcacaa gtgcttccaa atggttattg
     3721 tagattgcca gtagtagcag gtggttgtcc acatgcgaat gcatgcttag attgcactca
     3781 cttctgtacc agtaagcaat tcttaccaca gcacgaagaa cagttagagc gtacagaaga
     3841 gttattagcc atagctaagg ataaacaatg gcaaagacaa gtagagacta atagccgtgt
     3901 taaagagcgt ttagaacaaa tcattggaag tttgacgggg taattatcaa tggataaaca
     3961 agttagaaat acaacagaaa ttgtacgttt ggcgaagcag aaatcaaaaa agacaaggga
     4021 aaaagtagac aaagcgattt ctaaattttc gattgaaggt aaagttatta attttaattc
     4081 aatagcaaag gaagctaatg tttctaaatc atggctttat aaggaacacg atattaggca
     4141 aagaatcgaa tcccttcgtg agcgtcaaat aacagcaaat gtagtctcaa aacccaagaa
     4201 aagttctcgt tcggaggaaa tccttattaa aaccttaaaa agaagagtaa tggaattaga
     4261 aaaagaaaat aaaaaattac agaaccaaat tcaaaaatta tatggagatc tgtataataa
     4321 agaataatta ttaatctgta gacaaattgt gaaaggatgt acttaaacgc taacggtcag
     4381 ctttattgaa cagtaattta agtatatgtc caatctaggg taagtaaatt gagtatcaat
     4441 ataaacttta tatgaacata atcaacgagg tgaaatcatg agcaatttga ttaacggaaa
     4501 aataccaaat caagcgattc aaacattaaa aatcgtaaaa gatttatttg gaagttcaat
     4561 agttggagta tatctatttg gttcagcagt aaatggtggt ttacgcatta acagcgatgt
     4621 agatgttcta gtcgtcgtga atcatagttt acctcaatta actcgaaaaa aactaacaga
     4681 aagactaatg actatatcag gaaagattgg aaatacggat tctgttagac cacttgaagt
     4741 tacggttata aataggagtg aagttgtccc ttggcaatat cctccaaaaa gagaatttat
     4801 atacggtgag tggctcaggg gtgaatttga gaatggacaa attcaggaac caagctatga
     4861 tcctgatttg gctattgttt tagcacaagc aagaaagaat agtatttctc tatttggtcc
     4921 tgattcttca agtatacttg tctccgtacc tttgacagat attcgaagag caattaagga
     4981 ttctttgcca gaactaattg aggggataaa aggtgatgag cgtaatgtaa ttttaaccct
     5041 agctcgaatg tggcaaacag tgactactgg tgaaattacc tcgaaagatg tcgctgcaga
     5101 atgggctata cctcttttac ctaaagagca tgtaacttta ctggatatag ctagaaaagg
     5161 ctatcgggga gagtgtgatg ataagtggga aggactatat tcaaaggtga aagcactcgt
     5221 taagtatatg aaaaattcta tagaaacttc gtaagcgccc catagaacag tacctgtacg
     5281 tcaaataacc cgacctatag aatagaatcg ggaatgtttg gcgtttttag ttacgagaaa
     5341 aatgttgttg tacatccttg ttccaaggca acagttgatc taaagtttct gctgaaagta
     5401 cgggaagatt tggtatctta tcaaatagaa actctagata ctttacagga ttcaatccgt
     5461 taagttctgc tgttttatat acactcagaa tgattcctga agttcgtgct ccttcaagac
     5521 ttgtggaatg caaccaattt ttccgtccta tcaccagctc tttgatattt cgctccgcac
     5581 gattatttgt gagttctaaa cgaccatctt caaaaatagg ttcaaaacca cttcgtagtt
     5641 tacacgcata ttgaacggct gcatccaact tggattttgc aacaggatct atcgttgcca
     5701 tccactcata aaaagcaata aataatggac gcagttcttg ttgccgacag cttaatcgct
     5761 tctcaggaga aaagtctttc catttctttt ctaggacaaa ccatttatcc aattgtttga
     5821 ctgcttgagc tgcgggaata tccgtgtttt tctttccttt tggaattgct tcgaaaaatt
     5881 tccgccgaac atgcgcgaaa caatagacaa gtcgtacact ttccacggca ttatacccgc
     5941 tatagccgtc acagtgtaaa taccctgtaa actccttgag aaaagttttc ggaacatctc
     6001 cagcacgact ttcgtcgtgg tgataataaa cgatgggatg ctgactgtct tttcctgtac
     6061 aaaactgcca gtaataggta tctgttttgg cactttcaat cacacgataa cttgtttcat
     6121 ccccgtgtag aatctcttct tgattaagat agactcttaa tcgttctcct aaagggtcta
     6181 acgcgttttg cactgcttta atatgccaat tggtgatatt atctctcgaa atatccaacc
     6241 catgtgtttg tttccaatag ttctcttgcc gataggcagg aactttctga ccaaatttca
     6301 tccggatcgt ttccgccact aaactattgg aacctaagct attagaaatc aaaggtttag
     6361 gaacgatcgc ttttttaaaa gaagaaagtc cttctttttc gcagctttta caacaatagg
     6421 tatgttgata atgggtgaga caatccaaca tagcctgatg gaaacgaatt tcttctcgta
     6481 cttttgtccg tccgatatct ttcatctgtg aaccgcaatg ttcacaaaat tgttcttcca
     6541 aaggaaggag atgttctttc tctgttatag gtaagtgttt gattttgtct gctttcgttc
     6601 ctacaactcg ttgatgtcct tttactggaa taatggtggc agctgcttct ggaacactta
     6661 cagaaatttc ttcatcacta aaaagattca attgcccatt cgtttcctca aagagagtct
     6721 ctttagagcg tccaaataat ttgttggtta aataattgac cttttcattc aatagttgga
     6781 tttcagttgt aagattctga atggtttgtt gttgtgcttg attggtttta tgggcttctt
     6841 gaagcaactg aagcaacgta tctgtagttt ccaaaatttt tcaccacctt gaatcgaact
     6901 gattttgtta cagaatacca caagaaacgc acaagaaata ggggttcaac acattttttg
     6961 tgttaacccc ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc
     7021 gatacttaat ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc
     7081 tttcgtccga ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc
     7141 atcaccgtcc caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac
     7201 ggcatcttcg taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat
     7261 tccttttcgc aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc
     7321 ataaagcatt ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt
     7381 tatagatcaa aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg
     7441 cgggtttacc tttttctggt gcactctttt cgtttcctaa ttggacagga acaatctcat
     7501 ttaatttata catagaaaac ctccagactt cttgatagct caagtatatc ttgaggtgca
     7561 tttgttagta aggtactgtt ctatggggcg cttac
//
LOCUS       DACENJ010000386         5660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.guided.161, whole genome shotgun sequence.
ACCESSION   DACENJ010000386 DACENJ010000000
VERSION     DACENJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.guided.161"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1213
                     /locus_tag="IW748_002769"
     CDS             245..1213
                     /locus_tag="IW748_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1105650.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IW748_002770"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IW748_002770"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1105651.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKKKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IW748_002771"
     CDS             2243..2851
                     /locus_tag="IW748_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1105652.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4190
                     /locus_tag="IW748_002772"
     CDS             3279..4190
                     /locus_tag="IW748_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1105653.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4343..4828
                     /gene="vanZ"
                     /locus_tag="IW748_002773"
     CDS             4343..4828
                     /gene="vanZ"
                     /locus_tag="IW748_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1105654.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5115..5444)
                     /locus_tag="IW748_002774"
     CDS             complement(5115..5444)
                     /locus_tag="IW748_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1105655.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
BASE COUNT         1809 a          979 c         1204 g         1668 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaaaaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc
     3361 aggaaaaagt cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg
     3421 ggacttctga aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc
     3481 tgctattaat caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga
     3541 atttatctaa acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata
     3601 tgtcaaaaga aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg
     3661 ttagtcattt tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc
     3721 aagaaatggg ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat
     3781 cactagatgt aggatcaagc ttgacgaaaa tggaacgagc ccctgaagga aagtggatag
     3841 aagaaaatgc ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa
     3901 caggaattca atatgaacca tggcatattc gctatgttgg tttaccacat agtgcgatta
     3961 tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca
     4021 tttctgttag tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata
     4081 ccaccattca tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg
     4141 taattgtgac agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga
     4201 atgaaaccaa cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag
     4261 ctttttaatt taaaacccac gttaactagg acattgctat actaatgata caacttaaac
     4321 aaaagaatta gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata
     4381 tttagtgaca ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt
     4441 taattatcat caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga
     4501 gatgatagat aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa
     4561 agaaatcgga tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat
     4621 aattcaattt atcttcgcta ttggagcgac agacataaca gatgtaatta caaatactgt
     4681 tggaggcttt cttggactga aattatatgg tttaagcaat aagcatatga atcaaaaaaa
     4741 attagacaga gttattattt ttgtaggtat acttttgctc gtattattgc tcgtttaccg
     4801 tacccattta agaataaatt acgtgtaaga tgtctaaatc aagcaatctg atctttcata
     4861 cacataaaga tattgaatga attggattag atggaaaacg ggatgtgggg aaactcgccc
     4921 gtaggtgtga agtgagggga aaaccggtga taaagtaaaa agcttaccta acactatagt
     4981 aacaaagaaa gcccaattat caattttagt gctgaggaat tggtctcttt aataaatttc
     5041 cttaacgttg taaatccgca ttttcctgac ggtaccccca aactaagatt tcatagatag
     5101 attttctgat aactttaaaa aacacgaata gcggaaagga tgtcttcatt cgctaaagag
     5161 taataaacta cttttccatc ttgacgtgat tttgccattc catgtttttt taaaaaacgt
     5221 aaatggtgtg acgttgtagc gacactagca gtaagtattt ctgctaaatc acagacgcac
     5281 atttccttat aggtttctaa agcatagaaa attttaattc tagaaaaatc gctgaaacat
     5341 ttccctaaca caagtaagtt tgaaaaatct ttttgatcca atttattttt tattccattt
     5401 actttttctt tatgtacgac agtcacttgg caaacttttt tcattcatct cacctcacat
     5461 tcaaacgatt atttgaatga agtataccta tttgatcact cttttgtcaa tctaccttaa
     5521 ttccaataca ctattataag caatttcatg tattgaagta aacttacttg caagtgcgtt
     5581 tttgattcca agttaaaaac atattattat tctacataat ttcctaatca aataaaactc
     5641 ctttgaaaat tgttctaaag
//
LOCUS       DACENJ010000387         5660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.guided.162, whole genome shotgun sequence.
ACCESSION   DACENJ010000387 DACENJ010000000
VERSION     DACENJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.guided.162"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1213
                     /locus_tag="IW748_002775"
     CDS             245..1213
                     /locus_tag="IW748_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1105656.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IW748_002776"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IW748_002776"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1105657.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IW748_002777"
     CDS             2243..2851
                     /locus_tag="IW748_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1105658.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4190
                     /locus_tag="IW748_002778"
     CDS             3279..4190
                     /locus_tag="IW748_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1105659.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4343..4828
                     /gene="vanZ"
                     /locus_tag="IW748_002779"
     CDS             4343..4828
                     /gene="vanZ"
                     /locus_tag="IW748_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1105660.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5115..5444)
                     /locus_tag="IW748_002780"
     CDS             complement(5115..5444)
                     /locus_tag="IW748_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1105661.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
BASE COUNT         1808 a          979 c         1205 g         1668 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc
     3361 aggaaaaagt cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg
     3421 ggacttctga aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc
     3481 tgctattaat caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga
     3541 atttatctaa acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata
     3601 tgtcaaaaga aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg
     3661 ttagtcattt tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc
     3721 aagaaatggg ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat
     3781 cactagatgt aggatcaagc ttgacgaaaa tggaacgagc ccctgaagga aagtggatag
     3841 aagaaaatgc ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa
     3901 caggaattca atatgaacca tggcatattc gctatgttgg tttaccacat agtgcgatta
     3961 tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca
     4021 tttctgttag tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata
     4081 ccaccattca tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg
     4141 taattgtgac agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga
     4201 atgaaaccaa cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag
     4261 ctttttaatt taaaacccac gttaactagg acattgctat actaatgata caacttaaac
     4321 aaaagaatta gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata
     4381 tttagtgaca ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt
     4441 taattatcat caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga
     4501 gatgatagat aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa
     4561 agaaatcgga tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat
     4621 aattcaattt atcttcgcta ttggagcgac agacataaca gatgtaatta caaatactgt
     4681 tggaggcttt cttggactga aattatatgg tttaagcaat aagcatatga atcaaaaaaa
     4741 attagacaga gttattattt ttgtaggtat acttttgctc gtattattgc tcgtttaccg
     4801 tacccattta agaataaatt acgtgtaaga tgtctaaatc aagcaatctg atctttcata
     4861 cacataaaga tattgaatga attggattag atggaaaacg ggatgtgggg aaactcgccc
     4921 gtaggtgtga agtgagggga aaaccggtga taaagtaaaa agcttaccta acactatagt
     4981 aacaaagaaa gcccaattat caattttagt gctgaggaat tggtctcttt aataaatttc
     5041 cttaacgttg taaatccgca ttttcctgac ggtaccccca aactaagatt tcatagatag
     5101 attttctgat aactttaaaa aacacgaata gcggaaagga tgtcttcatt cgctaaagag
     5161 taataaacta cttttccatc ttgacgtgat tttgccattc catgtttttt taaaaaacgt
     5221 aaatggtgtg acgttgtagc gacactagca gtaagtattt ctgctaaatc acagacgcac
     5281 atttccttat aggtttctaa agcatagaaa attttaattc tagaaaaatc gctgaaacat
     5341 ttccctaaca caagtaagtt tgaaaaatct ttttgatcca atttattttt tattccattt
     5401 actttttctt tatgtacgac agtcacttgg caaacttttt tcattcatct cacctcacat
     5461 tcaaacgatt atttgaatga agtataccta tttgatcact cttttgtcaa tctaccttaa
     5521 ttccaataca ctattataag caatttcatg tattgaagta aacttacttg caagtgcgtt
     5581 tttgattcca agttaaaaac atattattat tctacataat ttcctaatca aataaaactc
     5641 ctttgaaaat tgttctaaag
//
LOCUS       DACENJ010000388         3698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.guided.214, whole genome shotgun sequence.
ACCESSION   DACENJ010000388 DACENJ010000000
VERSION     DACENJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.guided.214"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..293
                     /locus_tag="IW748_002781"
     CDS             <1..293
                     /locus_tag="IW748_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAQ1105662.1"
                     /translation="IQKESYCLKTPYGNLKNVELFLFKMDSKWDKYIYRSKEVFLTGS
                     YNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVWNPKQLSEQGAENEFAEVH"
     gene            687..1184
                     /gene="dfrG"
                     /locus_tag="IW748_002782"
     CDS             687..1184
                     /gene="dfrG"
                     /locus_tag="IW748_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1105663.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1256..3208)
                     /locus_tag="IW748_002783"
     CDS             complement(1256..3208)
                     /locus_tag="IW748_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1105664.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3215..3511)
                     /locus_tag="IW748_002784"
     CDS             complement(3215..3511)
                     /locus_tag="IW748_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1105665.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1194 a          614 c          622 g         1268 t
ORIGIN      
        1 atattcaaaa ggaaagctat tgtttaaaaa caccttatgg aaatttaaaa aatgttgagc
       61 tgtttttatt taaaatggat agtaagtggg ataaatatat ctatagaagt aaagaagtat
      121 ttttaactgg tagttataac aaaactcttc aggagagcct gtacgacaca ggagcaatat
      181 ttaataatga acctatggtt ttgaaagatt tctttggaga ttatgaacta gtttggaatc
      241 caaaacagct tagtgaacag ggggcagaaa atgaatttgc tgaagttcac taacatagtg
      301 tacagaaaaa cattatctga aagaagaacg ccttcaaaag aaagtacaag aacgattggg
      361 ccgtacagaa gactaacgaa agatcgtaaa aaaaaggaaa gacaatgaaa attaagtagt
      421 tcagttttgg agtacaaaat gacaaaatgt acttcaaaat ggactacttt tttcatttta
      481 acttacatac caaattacat acaaaatgat ttataaaatg caattgaaaa tgataattga
      541 atgaaaattt gtgattttgt ggtattatga taatataact tatagaaaaa attgcttatt
      601 caactaacgg gcaggataat gagaaaaggt tttttgatag aaataagaaa ttagatggta
      661 aattagttaa aaaggctgtg ttataaatga aagtttcttt gattgctgcg atggataaga
      721 atagagtgat tggcaaagag aatgacattc cttggaggat tcccaaggac tgggaatatg
      781 ttaaaaatac tacaaaggga catccgataa tattaggtag gaagaacctt gaatcaatcg
      841 gaagagcctt acctgacaga agaaatatta ttctgacgag agataagggg tttaccttta
      901 atggttgtga aattgttcat tcaatagaag atgtttttga gttatgtaaa aacgaagaag
      961 aaatttttat tttcggagga gaacagattt ataatttgtt tttcccttat gttgagaaaa
     1021 tgtacatcac aaaaatacat catgaattcg aaggagatac tttttttcca gaagtgaatt
     1081 atgaggaatg gaatgaggta tttgcccaaa aagggataaa gaatgataaa aatccgtata
     1141 actactattt tcatgtatat gaaagaaaaa acttattgag ttaacgggtg cgttaattga
     1201 agaagaacag taaagataaa gcacaagagc ttgggattac tccaagctct ttttgtcatt
     1261 tgaaataaat tatatctttc caatcaaatt gtttatctgt aatccctaat ctttgagcag
     1321 gtgtcaaaac ctttttatcc gctgatttaa aagggcgaca aaagttgtag taagttctta
     1381 gaattgtaat cgcaaattga gcatacttcg gattgaagtt agcgtaaata tagctcttct
     1441 tatcaccacg agctgttgtt aatggtcttt caagagatga tatataacgt cttatctgtt
     1501 gaataaagct attggtcgaa tggtcattaa catttaatag catctttgcg atttcgtttg
     1561 gttccagagc agataaatcc gttctacaat caactgaata aaatccttta tccttagtgg
     1621 ctaatggatg tttgataggg ttatcggcat atttgcgata tctttctcca tctttactaa
     1681 tagcctcttc gtggaattga tgacttgtta gtaattcagt tagataatga gatgccaaag
     1741 tccgtagaaa ctttgttttg aatcctctga tatctcccca atcgagcagt tctgcttttg
     1801 cttgtccgaa ttcttttaag cattgttttc tgcttttcgt cttgttaact tggcaaataa
     1861 aatgatgtgc atcgctcaac cttaattctt tcgtgaatac tctataaaag gcgtttctaa
     1921 tagagaagtc atcatcacta atcattcgcc attcgtcggc attaaccatt tgtttaatta
     1981 accaatagtg agcagttgta gtataaggag catcaacatt taaaccatcg acaaatctac
     2041 tcctattagt aattttccct aattcgtgaa gataagcatt cctattttcg ctatcgttag
     2101 cggatggttc ttgtggataa taagaccaat ctaacctatc atttttacga ctaaaagtat
     2161 tgagatggtc ttctttgaat tttcttgtat cttcatttaa ttcatccatt gaaatattcc
     2221 aatcataggc aacatcagaa cggaacacat atcttgataa gacctcagca gatacaacta
     2281 tatacgtttg catattaagg tcttcaaacc ccgtatattt ctttgaacct tgtcctttct
     2341 tacggacgtt atttaggtag tagtgcatct tgtccgtatt aagccacatc tcgttaaatt
     2401 tctttgtttg aaggggttgt gtctcatatc gttctaaaaa ctccaaacaa cgcctataca
     2461 gccactccag cttatgatag taggttccaa ctccaattcc taaaatatca caagtacgac
     2521 tgactgaaac ccttccaaca accatcttag cgaacattgg aagaatggtg ttcttttgtt
     2581 ggtgataagt agtggtttct tctctttttg gtagaacatt agtgaacttc ttacacgcct
     2641 tacattgata gcgttgagat ttgcccctac ttttccccct tttgtaaaat tgcttcggct
     2701 cattgaatgg ggttgattct tcttccgagc agccttcttt atgaaattga taatctggct
     2761 caacatcttg gatgctattg atttctataa ggcgtttaat ttcttcaaca acagaccaat
     2821 tagataaagg ggttactgta cagttttgcg atacacctct gccaatagga ttgtcattgc
     2881 agtacatacc tttatgacct ttatctttgg aactaccagt cattttatat cgacttggtt
     2941 tcccttttac atctttgaat ttgtgctgtt ctttcccaaa attacaacaa aacggattga
     3001 tgcagtaatt atattgaatg ggatattcag aaccgttcca tttaaaagac gttggcaaga
     3061 acaacatttt gttatacctt gttgcaaatc gttttggagt taataaatca tattgtttag
     3121 ccctatcttt taattcttcg tttgaaatcg gaattttaac atctacaatt tcatcttcct
     3181 tcgtggctaa tcttttaagc ttggtcattg tctatcaacc cactctatct ttgataccca
     3241 ttttttcaac aatccgctta ttacttcgct tgttggcaat ccattcaata aacttcttat
     3301 cgtctaaaat gttcaataag aattcatcca ctgcttcgct ttcagtacgc tctgcatatt
     3361 cagcgtattg cttaactatc acccttactt gctctgatat ttcccaatcc acagacttag
     3421 cgttcttatt ttttggctca agaaattgca taggaaaatg cacccctttt tgcttagtaa
     3481 attgtaacca ttttaacaca ccaatttgca ttcctacaat aattatttca cgttcgcatt
     3541 tttatataca ttttaactta cattttgtgg tgtaaattaa caacaaacgt tgatttaaca
     3601 ataaaaaacg agtatcaaat tcagttgcaa tttgatactc atttaggttt acattttcac
     3661 tttttgtact ccaaaactga actacttaaa tgaaaatg
//
LOCUS       DACENJ010000389         2697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.guided.249, whole genome shotgun sequence.
ACCESSION   DACENJ010000389 DACENJ010000000
VERSION     DACENJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.guided.249"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..282
                     /locus_tag="IW748_002785"
     CDS             <1..282
                     /locus_tag="IW748_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1105666.1"
                     /translation="AVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEHCEM
                     DHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            266..895
                     /locus_tag="IW748_002786"
     CDS             266..895
                     /locus_tag="IW748_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1105667.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1249..>2697
                     /gene="msr"
                     /locus_tag="IW748_002787"
     CDS             1249..>2697
                     /gene="msr"
                     /locus_tag="IW748_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1105668.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQK"
BASE COUNT          941 a          457 c          575 g          724 t
ORIGIN      
        1 gcagttactc gtcatctgaa ggtattggaa gcaaatggct atgtttcgcg tcagagaaat
       61 cctcagaaca accgggaagt gtatgtagcg ctaacagaaa aagcccagaa gaaaatcgag
      121 cattgtgaaa tggaccataa agatacaggt tccgtgctat ccccggcttt cagcgatgta
      181 gaaatgaaac aatttctaac acttttagat aaattgaata cccaactttg caatataaca
      241 acagaaacga aagaggttaa taataatgga acaaaaaaat gatttttcaa caattatgac
      301 tggtagaaga tccgttcgcg tatatgatga aacatacaaa atcccacacg aagaaatgct
      361 ggaaatgatc aaagaagcga cgactgcacc ttcttctgtc aatatgcaac catggcgctt
      421 tgtggtcgct gaaagcgagg aagcaaaaga aatcttgcgt cctttgatcc gtttcaatac
      481 gagacaaaat gatacttctt ctgcaatggt catgattttt ggcgatatgc aatgttacga
      541 atatggcgaa gaaatctata accaagctta cgaatcaggg aagatgccaa aagaagtcag
      601 agatcaacaa ttagctgcga tcattcctta ttacaagagt ttttcaagag aagaaatgaa
      661 cgatgtagtc aaagtcgatt ctagcttagc ggctatgcaa ttcatgttag ttgcgcgtga
      721 tcatggctat gaaacaaatc caattggtgg attcgaagca gatcagttgg cagaggcatt
      781 tggtttggat aaagaccgtt atgttccagt tattatttta tcaataggaa aagcggtaga
      841 agaaggttac gagtctgttc gtttagcacc agaaaagatc actacgttcg aataaaaggt
      901 tttcagttac tattcagtag ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa
      961 aaggaggtcg gaaaaatgac tgcatcgatg aaattacgtt tcgaactttt gaataacaac
     1021 taaataaatt caaaagacat gtataaacaa caatttcatg gatggagtat gtccattttt
     1081 tcgaacgaca gtagaatagg tgaaagctat gacagatttg gtgatttagt catagttatt
     1141 tcgttcagag taggaaaaga tgtgatgcag tttttttgca aacagatttt tctgctcttt
     1201 tttagtatcg ataattgttg ttttacatga aataggagtg taaaacatat ggaaaattta
     1261 gcagtaaata taacaaatct acaagtcagc tttggcaacc agctagaact atctattgat
     1321 tctcttcgtg tctatcagca agatcgtata gggatcatag gagaaaatgg tgtcggtaaa
     1381 tcgactttgc tcaaactaat agccggtgaa ctttttcccg atcatgggaa aatccaaaca
     1441 gagatcacct tcaactacct gcctcaatta acctatctcg ctgaagcaaa ggacctaaat
     1501 ttggaattag ccagtcattt ccagttaaga ctggaagaaa cttcagagcg gaaatggagc
     1561 ggaggggaag aacgaaagat cgagttgata cgtcttcttt cttcttatga acaagggatg
     1621 cttctagacg agccaacaac ccatctagat agaaaaagta ttgatcgact gattgaagag
     1681 cttcgttatt attatggtac gctggttttt gttagtcatg atcgctattt tctagatgag
     1741 ctagcatcga aaatctggga agtaaaagac ggagaaatcc gagagttttc ggggaattat
     1801 agtgcctatc tcactcaaaa ggaattggag aaaaagactc agctacgaga agcagagtcg
     1861 atcatgaaag agaaaaaacg attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa
     1921 aagttggaaa aagtgtccag taaaaagaaa aagcaacaaa tcagaccgga tcggttgtct
     1981 tcctctaaac aaaaagacag tgtacaaaaa gccatccaaa agaatgcgaa aacattagag
     2041 agaagactcc aaaaaatagg agaaacaacc aagccgcagc agatgaaaca aatccgtttt
     2101 ccagtaccaa aatctcttga actccacagc cgctatccca tcatgggaca aaatgtccaa
     2161 ttggaaagaa gtgggagaac attactggta aatggtgatt ttcagttttc tttaggtaaa
     2221 aaaatcgcga ttgtcggtga aaatggttca ggtaagacaa ccttattaga acatatccgc
     2281 aaacaaggag aaggaatcct tctctctccg aaagtaagct ttcaagtata tcagcaaaag
     2341 ggttatcaaa tgacatctga agaatccatc attcgttttg tcatgagaca aacagagttt
     2401 tcagaatcgc ttgtccgtag tttgctgaat cacttagggt ttgctcagga aactttgacg
     2461 aaaccgttat gtacattaag tgggggagaa gcgacccgtc tgacgattgc tttgcttttt
     2521 actaagccaa gtaatgtgtt gctgttagat gaaccgacta attttattga tatggcaacg
     2581 atcgaagctt tagagaagct aatgcaaata tatccgggaa cgattttgtt tacttctcat
     2641 gattcttact ttgtcgagcg tacggctgat gaagtttatg aaataaaagg gcagaaa
//
LOCUS       DACENJ010000390         2253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.guided.274, whole genome shotgun sequence.
ACCESSION   DACENJ010000390 DACENJ010000000
VERSION     DACENJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.guided.274"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            26..1201
                     /locus_tag="IW748_002788"
     CDS             26..1201
                     /locus_tag="IW748_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1105669.1"
                     /translation="MILCTAMVCLMTPGLAFFYGGLARKNNILTIMSQSFLSVGITTI
                     MWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPFLMFFLYQLMFIVI
                     TVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQMGFRDFAGGAVIH
                     TTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWFGFNSGGALRADEQ
                     AVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAAITPCAGYVEAKSA
                     MLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIGIFASNSHLLTNPS
                     SWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEEGLDFFFFGNKLN"
     gene            1232..1450
                     /locus_tag="IW748_002789"
                     /pseudo
     CDS             1232..1450
                     /locus_tag="IW748_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1869..>2253
                     /locus_tag="IW748_002790"
     CDS             1869..>2253
                     /locus_tag="IW748_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1105670.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPE"
BASE COUNT          670 a          362 c          515 g          706 t
ORIGIN      
        1 tatcaataca ggagatacag cattcatgat tttatgtaca gcaatggttt gcctgatgac
       61 tcctggtcta gctttttttt acggcggatt agcacggaag aataatatac taacgatcat
      121 gtctcaatct tttctctccg ttgggataac aacgattatg tgggtatttg gtggattcgg
      181 attggccttt ggacgggata tcggcggtgt tattggaaac ccggcagatt tctttttgat
      241 gagacatgtt acaagcttcc ccaacatgtt tcatggtgca acgattccat ttttaatgtt
      301 ctttctttac caactgatgt ttatcgtgat taccgttcct ttgatgacag gagcttttgc
      361 agggagattg aatttaaaag gctatattat acttgttatt ttttggaatc tgctgattta
      421 tttccctgtt tgccactgga tatggggtgg cggatttttg gatcagatgg gatttagaga
      481 tttcgctggt ggagcagtca tccacacgac agctggattc ggctctttag catgtatcct
      541 cacacttggt caaagaaaga ttcctttagg agaaagaagt cagcatagca gtcttatggc
      601 agctgctatc ggtacaggac tgctttggtt tggctggttc ggttttaatt ccggtggtgc
      661 acttcgagca gatgaacaag cagtcaatgc atttggttca acattcatcg ctcttgcatt
      721 tgcgatgatt acttggctga tcattgcaaa agtaaaagga aatggctttg attttgtcga
      781 cgtactaaca ggatccgttg ctggattggc agccattacg ccttgtgccg gttatgtaga
      841 agcaaaaagt gccatgctga ttgggatcat tgcgggtatc gtctgtcatg ctgctgttga
      901 ttttcgtaaa aagaaacaat gggatgatgc acttgatgtg tggggtgttc atggcatggg
      961 cggattcaca ggtacgattc tgatagggat ctttgcatct aacagccatt tactaacgaa
     1021 tccaagcagc tggtacttct tagggattca agtgattggt gtcatcatta ctgccgtgta
     1081 tgcctatgtg ctgacaacag tgattttaaa atcggcgatg cactttacaa cgatcactac
     1141 tacaaaagaa gaacaagaag aaggattgga ctttttcttc tttggcaaca aactcaattg
     1201 aaacaaattc aaatagaaaa gaggaagatt catgcaacaa agcaaaacga aaattcctgc
     1261 aaaaaaatta acgttctttg gctttttggc tatgaccatc tcaatggtcg tttcattata
     1321 tgaatatccc acattcgcga cctcaggctt cagcttagta tttttcttgt tattaggcgg
     1381 tttgctatgg tttatccctg tggcattgtg cgcagcagag atggcaacgg tgaaaggatg
     1441 ggaaaaaggt gtttcccaaa caaaaagata ttaagaaaca gcccatgttt acctgcttgc
     1501 aaacaaaaga atattgctac tagactaaga gatactacat aagtgtgttg gtatctcttt
     1561 tttgttgccg tgaaacagca gactatttca tttctgcttg attaccttaa aacttattag
     1621 tctatggttt gtcaagggct tgttgcgtga gaaatgctga atatcaccct ttacaaaaca
     1681 taggctgata agtaactttt caaaagcaga aaagaaatgg caatgcttat aacaatatga
     1741 tataatgcag atgaagacaa aaaaggaatt tgtcgagctg gttagttcga gataattttg
     1801 agtttgttaa gatgtggtgc acttgatgga atcacaggta cgacatcata ttatggagga
     1861 tgaaatagat gataggtttg attgttgcga ggtcaaagaa taatgttata ggcaagaatg
     1921 gtaatatacc atggaaaata aagggagaac aaaagcaatt tagagagtta acaacgggta
     1981 atgtggttat tatggggcga aagtcttatg aagaaatcgg tcatccgttg cctaatagaa
     2041 tgaatattgt tgtttccacc acaacagagt atcaaggaga taatttagtt tcagttaaat
     2101 cattagaaga tgcattatta ttggctaaag gacgagatgt atacatatct ggtggatatg
     2161 gactatttaa ggaagctttg caaatagtag ataaaatgta tatcacagaa gtagatttaa
     2221 atattgaaga tggagataca ttctttccag aat
//
LOCUS       DACENJ010000391         2246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.guided.275, whole genome shotgun sequence.
ACCESSION   DACENJ010000391 DACENJ010000000
VERSION     DACENJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.guided.275"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..152
                     /locus_tag="IW748_002791"
     CDS             <1..152
                     /locus_tag="IW748_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1105671.1"
                     /translation="YLIVDLKLCNRTNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
     gene            366..1061
                     /gene="vanR"
                     /locus_tag="IW748_002792"
     CDS             366..1061
                     /gene="vanR"
                     /locus_tag="IW748_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1105672.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1039..2193
                     /locus_tag="IW748_002793"
     CDS             1039..2193
                     /locus_tag="IW748_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1105673.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          758 a          410 c          475 g          603 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa ttatgcggta aagctatata
       61 aagaaggaaa tatgactgta aatcaaattt gtgaaattac taatgtatct agggcttcat
      121 tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta atgggcaata
      181 tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat gccgaaaagc
      241 cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt atgtaaatgc
      301 ttataaattc ggccctataa tctgataaat tattaagggc aaacttatgt gaaagggtga
      361 taactatgag cgataaaata cttattgtgg atgatgaaca tgaaattgcc gatttggttg
      421 aattatactt aaaaaacgag aattatacgg ttttcaaata ctataccgcc aaagaagcat
      481 tggaatgtat agacaagtct gagattgacc ttgccatatt ggacatcatg cttcccggca
      541 caagcggcct tactatctgt caaaaaataa gggacaagca cacctatccg attatcatgc
      601 tgaccgggaa agatacagag gtagataaaa ttacagggtt aacaatcggc gcggatgatt
      661 atataacgaa gccctttcgc ccactggagt taattgctcg ggtaaaggcc cagttgcgcc
      721 gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc
      781 ttgtcattaa tgttaacacc catgagtgtt atctgaacga gaagcagtta tcccttactc
      841 ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg
      901 agctgctatt tcatgagata tggggcgacg aatatttcag caagagcaac aacaccatca
      961 ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac cattgataat ccgaaatata
     1021 taaaaacggt atggggggtt ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa
     1081 ctagaacgaa aactttacat gtatatcgtt gcaattgttg tggtagcaat tgtattcgtg
     1141 ttgtatattc gttcaatgat ccgagggaaa cttggggatt ggatcttaag tattttggaa
     1201 aacaaatatg acttaaatca cctggacgcg atgaaattat atcaatattc catacggaac
     1261 aatatagata tctttattta tgtggcgatt gtcattagta ttcttattct atgtcgcgtc
     1321 atgctttcaa aattcgcaaa atactttgac gagataaata ccggcattga tgtacttatt
     1381 cagaacgaag ataaacaaat tgagctttct gcggaaatgg atgttatgga acaaaagctc
     1441 aacacattaa aacggactct ggaaaagcga gagcaggatg caaagctggc cgaacaaaga
     1501 aaaaatgacg ttgttatgta cttggcgcac gatattaaaa cgccccttac atccattatc
     1561 ggttatttga gcctgcttga cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat
     1621 gtgcatatca cgttggacaa agcgtatcga ctcgaacagc taatcgacga gttttttgag
     1681 attacacggt ataacctaca aacgataacg ctaacaaaaa cgcacataga cctatactat
     1741 atgctggtgc agatgaccga tgaattttat cctcagcttt ccgcacatgg aaaacaggcg
     1801 gttattcacg cccccgagga tctgaccgtg tccggcgacc ctgataaact cgcgagagtc
     1861 tttaacaaca ttttgaaaaa cgccgctgca tacagtgagg ataacagcat cattgacatt
     1921 accgcgggcc tctccgggga tgtggtgtca atcgaattca agaacactgg aagcatccca
     1981 aaagataagc tagctgccat atttgaaaag ttctataggc tggacaatgc tcgttcttcc
     2041 gatacgggtg gcgcgggact tggattggcg attgcaaaag aaattattgt tcagcatgga
     2101 gggcagattt acgcggaaag caatgataac tatacgacgt ttagggtaga gcttccagcg
     2161 atgccagact tggttgataa aaggaggtcc taagagatgt atataatttt ggctctttgt
     2221 caaatgatgt tggtaaagag aaatct
//
LOCUS       DACENJ010000392         1998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.guided.300, whole genome shotgun sequence.
ACCESSION   DACENJ010000392 DACENJ010000000
VERSION     DACENJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.guided.300"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            47..439
                     /locus_tag="IW748_002794"
     CDS             47..439
                     /locus_tag="IW748_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1105674.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="IW748_002795"
                     /note="aph(2'')-Ia"
     CDS             440..1879
                     /locus_tag="IW748_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ1105675.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          831 a          153 c          343 g          671 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaatga aaaataataa aggaagtgag tcaagtccag actcctgtgt
     1981 aaaatgctat acaatgtt
//
LOCUS       DACENJ010000393          979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E345
            SAMN07141434-rid9870233.guided.406, whole genome shotgun sequence.
ACCESSION   DACENJ010000393 DACENJ010000000
VERSION     DACENJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141434
            Sequence Read Archive: SRR5811429
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740759.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740759.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:19:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 12
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 6
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 14 of 48
            Pseudo Genes (internal stop)      :: 15 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141434-rid9870233.guided.406"
                     /isolate="E345"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            96..827
                     /gene="erm(A)"
                     /locus_tag="IW748_002796"
     CDS             96..827
                     /gene="erm(A)"
                     /locus_tag="IW748_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAQ1105676.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT          376 a          158 c          154 g          291 t
ORIGIN      
        1 ttattaataa agttcgttat caaccaaatc aaaattaatt ggttataatg aacgcttaat
       61 gtcagttcat tataaccagt aaggagaagg ttataatgaa ccagaaaaac cctaaagaca
      121 cgcaaaattt tattacttct aaaaagcatg taaaagaaat attgaatcac acgaatatca
      181 gtaaacaaga caacgtaata gaaatcggat caggaaaagg acattttacc aaagagctag
      241 tcaaaatgag tcgatcagtt actgctatag aaattgatgg aggcttatgt caagtgacta
      301 aagaagcggt aaacccctct gagaatataa aagtgattca aacggatatt ctaaaatttt
      361 ccttcccaaa acatataaac tataagatat atggtaatat tccttataac atcagtacgg
      421 atattgtcaa aagaattacc tttgaaagtc aggctaaata tagctatctt atcgttgaga
      481 agggatttgc gaaaagattg caaaatctgc aacgagcttt gggtttacta ttaatggtgg
      541 agatggatat aaaaatgctc aaaaaagtac caccactata ttttcatcct aagccaagtg
      601 tagactctgt attgattgtt cttgaacgac atcaaccatt gatttcaaag aaggactaca
      661 aaaagtatcg atcttttgtt tataagtggg taaaccgtga atatcgtgtt cttttcacta
      721 aaaaccaatt ccgacaggct ttgaagcatg caaatgtcac taatattaat aaactatcga
      781 aggaacaatt tctttctatt ttcaatagtt acaaattgtt tcactaaatt aaagtaataa
      841 agcgttctct aatttcacaa gaggacgctt tattcttccc aaaaattgtt caatatttat
      901 caataaatca gtagttttaa aagtaagcac ctgttattgg taagcgcccc atagaacagt
      961 accttactaa caaatgcac
//
